title: GSM5653435: A3: A2058 [RSL3]; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA were extracted with Trizol (Tiangen, Beijing) and assessed with Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA) and Qubit Fluorometer (Invitrogen). A total amount of 3ug total RNA per sample was used as input material for the small RNA library. Sequencing libraries were generated using NEB Next®Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.)