title: GSM5519694: S. enterica-MacT interactions-CerroA_rep1 [Cerro_A_1]; Salmonella enterica; RNA-Seq
library_construction_protocol: MAC-T cells were infected with S. enterica strains for 2 h. The infected MAC-T cells were lysed in ice cold RNA stabilization solution, and bacterial pellets were harvested using centrifugation. Total RNA was extracted from bacterial pellets Quick-RNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA). Total RNA was treated with the MICROBEnrich™ Kit (Invitrogen) to eliminate/reduce MAC-T cell RNA contaminants. RNA samples were treated with DNAse. RNA libraries were prepared for sequencing using standard Illumina protocols