title: GSM5982479: HAEC_0dy_0h_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of the three groups of HAECs was extracted using TRIzol reagent according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructed using TruSeq Stranded Total RNA with Ribo-Zero Gold (Illumina, Cat. No. RS-122-2301) according to the manufacturer's instructions. RNA (1 μg) from each sample was used for library construction. First, ribosomal RNA (rRNA) was removed using Ribo-Zero Gold rRNA Removal Kit (Illumina), and RNA was fragmented using a fragment reagent. Subsequently, sequencing libraries were constructed using rRNA-depleted RNA. Finally, products were puriﬁed (Agencourt AMPure XP, BECKMAN COULTER), and library quality was assessed using the Agilent Bioanalyzer 2100 system.