title: GSM5702283: pLKO_6_19; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in RLT Plus lysis buffer and total RNA was extracted using the RNeasy Plus Mini kit (Qiagen) according to manufacturer's protocol. High RNA quality was verified using the Bioanalyzer RNA 6000 Nano assay (Agilent). Libraries were prepared from 500 nanograms of total RNA per sample using standard Illumina protocols