title: RNA-Seq of pooled fourth instar Entylia carinata; metathoracic leg
design: Total RNA extracted from pooled tissue. PolyA enriched with oligo(dT) mRNA capture magnetic beads. RNA chemically fragmented. Library prepared with Stranded mRNA Kit (KAPA), hairpin adaptors (NEB), and unique-dual indices (Berkeley Genomics Laboratory QB3)