title: DNA sequencing of C. elegans: coevolved w/ Staphylococcus only replicate 4 generation 15
design: 200 ng of DNA was sheared Covaris to ~550bp fragments. 18 libraries were pooled in equimolar amounts, loaded at 200pM concentration and sequenced on three lanes with v1 chemistry. Fastq files were trimmed using Cutadapt v.1.2.1 and Sickle v.1.200. Reads were aligned to Caenorhabditis elegans WBcel235 reference using BWA-MEM v.0.7.5a-r405 and duplicate reads identified and removed with Picard v.1.94.