title: GSM6122144: wce, rep2; Chlamydia trachomatis; OTHER
library_construction_protocol: C. trachomatis infected HeLa cells collected washed and collected in sonication buffer (1mMEDTA, 10mMTris-HCl (pH7.5), 0.1%SDS) and sonicated in Covaris M220 sonicatior Library constructed with NextFlexChIP-seq Kit with NextFlex barcodes from Bioo Scientific. binding reaction was carried in 30 µl with recombinant Euo (50ng), sheared DNA (1µg), 1 µg salmon sperm DNA, 9 µg bovine serum albumin in buffer B [20 mM Tris, pH 7.5, 100 mM NaCl, 10%(v/v) glycerol] for 30 min at 37˚C OTHER (DNA-Immunoprecipitation-seq, DIP-seq)