title: GSM5233363: RNAseq_WT_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNAs were extracted with Trizol following the manufacturer's recommendations 1µg RNA was used for library preparation. RNA-seq library preparation was performed as described in TruSeq Stranded Total RNA Sample Preparation kit from Illumina with few adaptations. RNA Clean XP beads and AMPureXP beads are air-dry for 7min. 1uL of libraries were used to determine concentration by Qubit DNA HS assay kit and to run Agilent High Sensitivity DNA Electrophoresis to check DNA fragment size. Before sequencing, RNA-seq libraries were quantified by qPCR. Libraries were sequenced 100bp single-reads with 30 million reads of depth sequencing on NovaSeq 6000 Illumina machine at the MGX platforms.