title: GSM5979851: Angel1 IP, rep 2; Homo sapiens; RIP-Seq
library_construction_protocol: Cell pellets were flash frozen in liquid nitrogen and stored in -80 degrees until ready to IP M2 FLAG antibody. Subsequent protocol was followed exactly as detailed in Blue et al 2021. Sequencing was performed on an Illumina MiSeq platform using single-end reads and data processed through Dr. Yeo's eCLIP pipeline. Libraries were constructed as per the eCLIP protocol using Illumina 50x forward and 70x reverse primers.