title: GSM5824285: Azole Resistant Progeny Pool BC6 rep3 [S6BCR3]; Aspergillus fumigatus; OTHER
library_construction_protocol: DNA was extracted using a Qiagen Plant DNA extraction kit following the manufacturer's instructions for adaptation for filamentous fungi. DNA quality and quantity for genome sequencing was then assessed by gel electrophoresis, Nanodrop and Qubit methodologies. Libraries were prepared from 100 ng to 1 ug of input DNA using the NEBNext Ultra kit (NEB, UK) following the manufacturer's standard protocol. Libraries were then pooled and sequenced using the MiSeq instrument with the MiSeq Reagent Kit v3 (600 cycles) (Illumina, USA) to generate 2 x 300bp paired end reads