title: GSM5468036: PRPF39 size-matched input, rep1; Homo sapiens; RIP-Seq
library_construction_protocol: HepG2 replicates were treated with 400mJ of UV using the CL-1000 UV Crosslinker, harvested in ice cold PBS and pelleted by centrifugation. Cell pellets were flash frozen in liquid nitrogen and stored in -80 degrees until ready to IP with PRPF39 bound Dynabeads. Subsequent protocol was followed exactly as detailed in Blue et al 2021. Sequencing was performed on Illumina Novaseq 6000 single-end reads and data processed through Dr. Yeo's seCLIP pipeline. Libraries were constructed as per the (s)eCLIP protocol using Illumina 50x forward and 70x reverse primers. seCLIP