title: GSM5272839: SjS22-CCR9; Homo sapiens; RNA-Seq
library_construction_protocol: Venapuncture from patient or control. Fresh peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation from Li-heparin blood. PBMCs were stained with fluorochrome-conjugated antibodies against CD3, CD4, CXCR5 and CCR9. CCR9+CXCR5- (CCR9+), CXCR5+CCR9- (CXCR5+) and CCR9-CXCR5- Th cells were sorted with Fluorescence-activated cell sorting (FACS) using BD FACSARIAIII and harvested into tubes with RPMI 1640 containing 10% FCS and 1% penicillin/streptomycin. Cells were lysed in RLTplus buffer (Qiagen) with 1% beta-mercaptoethanol. RNA was extracted using the AllPrep Universal Kit in QIACube (both Qiagen) TruSeq RNA Library Prep Kit (Illumina) according to manufacturer's instructions