title: GSM6250344: SG511-02REV-02 [QOBWO029A3]; Staphylococcus aureus; RNA-Seq
library_construction_protocol: RNA was extracted using the Monarch™ Total RNA Miniprep Kit (New England Biolabs) following the manufacturer's instructions and stored at −80 °C. Quality and quantity of total RNA were determined by agarose gel electrophoresis. Per replicate, a total amount of 400 ng RNA was subjected to rRNA depletion. For rRNA depletion and cDNA library construction the IlluminaTM Stranded Total RNA Prep Ligation with Ribo Zero Plus Kit was used according to the manufacturer's instructions. Libraries were sequenced as single-read (101 bp read length) on a NovaSeq 6000 platform (Illumina)