title: GSM4830531: pPIN1::TRH1-YFP root tips rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: ~1 mm sections of root tips from trh1, pPIN1::TRH1-YFP and pPIN2::TRH1-YFP transgenic lines were collected for RNA-Seq analysis. Total RNA was extracted using the Direct-zol RNA Miniprep (Zymo research, Irvine CA, USA) kit with an on-column DNase treatment according to the manufacturer's instructions. The quantity and integrity of RNA were assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Fischer Scientific, Waltham, MA, USA) and agarose gel electrophoresis. RNA-seq libraries were generated using the TruSeq Low Input kit according to the manufacturer's instructions (Illumina). Sequencing was performed on BGISEQ-500 platform instrument at BGI (Beijing Genomics Institute) in three biological replicates for each sample.