title: GSM4829253: BJ48hS1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using TRIzol™ Reagent (Invitrogen, Cat. 15596026) followed by DNA digestion with DNAseI (Thermo Fisher Scientific, Cat. AM2222) and a cleanup step using Quick-RNA Miniprep (Zymo Research, Cat. R1054). RNA concentration was determined using Nanodrop spectrophotometer (Thermo Fisher Scientific). Sequencing library was constructed by BGI using its protocol (Document #, SOP-SS-027; Version #, A0, Effective date: 2018-06-11)PolyA mRNA was purified from total RNA using the oligo(T) attached to magentic beads. The mRNA was then fragmented.  cDNA was then generated using random hexamer-primerd reverse transcription followed by the second-strand cDNA synthesis. The synthesized cDNA was then subject to end-repair, and 3' adenylated. Adapters were then ligated to the ends of these 3' adenylated cDNA fragments. The cDNA was further PCR amplified. The amplified cDNA was purified with Ampure XP Beads. The library was validated on the Agilent 2100 bioanalyzer. The double strand PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circular DNA were formated as the final library. RNA-seq using nanoball technology, sequening was conducted by BGI