title: GSM5702209: hind muscle unloaded treated with vehicle 1; Mus musculus; RNA-Seq
library_construction_protocol: Total mRNAs were extracted from gastrocnemius muscles of the three groups of mice Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional.