title: GSM4829136: hESC-HNF1A-HET: ES_797397; Homo sapiens; RNA-Seq
library_construction_protocol: Around 100 cells per genotype at day 25 of differentiation are dissociated and sorted for GFP using BD FACS Aria II cell sorter. Human embryonic stem cells with different HNF1A genotypes (WT, KO1, KO2 and HET) were differentiated into islet-like clusters of endocrine cells for 25 days in vitro. Clusters of islet-like cells were dissociated into single cells and insulin producing cells were purified by FACS sorting for INS-GFP. Cells were analyzed by single cell RNA sequencing. There are three WT and three KO.