title: GSM5469113: Oligodendrocyte-enriched cells from RalAB cKO mouse 2 at P10; Mus musculus; RNA-Seq
library_construction_protocol: Oligodendrocyte-enriched cells were isolated from P10 spinal cords of individual mice per genotype. Spinal cords were minced with a scalpel and the cells were enzymatically disaggregated with an enzymatic solution. The suspension of disaggregated cells was loaded into a FACS machine and the populations enriched in oligodendrocytes were selected by conditional expression of the tdTomato reporter gated for large forward scatter (FSC-A). Cells from each mouse were directly collected into individual tubes containing 0.5 ml of Trizol (Invitrogen). Tubes were stored at -80C until needed. RNA extraction was performed with Trizol as per manufacturer's instructions. RNA libraries were prepared for sequencing using Illumina's TruSeq Stranded mRNA.