title: GSM6249241: 7768E4, 28, Exposed, Exposed [scRNA-Seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen PBMCs were thawed, and count and cell viability were measured by Countess II. The cell viability exceeded 80% for all samples except PBMC samples from CC subjects, which had viability between 70-80%. For single cell (sc) RNA-seq, 200K cells were aliquoted, spun down, resuspended in 30 uL PBS+0.04%BSA+0.2U/uL RNase inhibitor, and counted using Countess II. GEM generation, post GEMRT cleanup, cDNA amplification, and library construction were performed following 10X Genomics Single Cell 5' v1 chemistry and quality was assessed using Agilent DNA tape screen assay.