title: GSM5825686: skeletal muscle satellite cells RNAseq WT rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions For library construction, we used a protocol as described before.(Zhou et al., 2015) The purified library products were evaluated using a Bioanalyzer (Agilent) and SYBR qPCR and sequenced on an Illumina Hi-seq2000 sequencer