title: GSM5587038: Flag.2_C; Mus musculus; RIP-Seq
library_construction_protocol: After IP, dynabeads were incubated with 70 µg Proteinase K in agitation for 30 min at 42 °C and then for 30 min at 60 °C. Inputs and native IP samples were also treated with Proteinase K following the previous guidelines. Total RNA was extracted with RNeasy® Plus Micro kit, following the manufacturer's instructions. RNA from RIP were used to construct libraries for sequencing with the TruSeq® Stranded mRNA Library Prep Kit, using the Dual index Nextera XT v2 Set A Adapters. Since RNA was already enriched due to IP, no purification and fragmentation steps were needed for library preparation, thus only random hexamers and not poly-T oligos were used during the process.