title: GSM5980044: WT-2; Mus musculus; RNA-Seq
library_construction_protocol: In vitro activated CD8 T cells were then washed and resuspended in 1 ml TRIzol and isolated following manusfacturer's recomendations. RNA was DNAse-treated using the TURBO DNA-free Kit (Thermofisher scientific). 30ng RNA from the spinal cord samples was used for downstream library preparation. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following manufacturer's recommendations. Final libraries were quantified and sizing was determined using the High Sensitivity DNA Assay reagents and chip in the Agilent 2100 Bioanalyzer System and the Qubit 1X dsDNA HS Assay Kit respectively. Individual libraries were then pooled and sequenced using 75bp paired end on the NextSeq 500 using the NextSeq 500/550 High Output Kit.