title: GSM5273580: patchMPRA_no_enhancer_DNA_rep1; Homo sapiens; OTHER
library_construction_protocol: DNA and RNA was harvested simultaneously using the TRIzol reagent (Life Technologies) following the manufacturer's protocol. RNA samples was first converted to cDNA by reverse transcription with the SuperScript IV First Strand Synthesis Systems (Invitrogen) using oligo dT primers. Barcodes were amplified from the respective plasmid/genomic DNA/cDNA samples using custom primers. Illumina sequencing adapters were then further added on 2 rounds of PCR with custom primers.