title: GSM4106057: Middle_aged_STD_rep2 [STD2]; Mus musculus; Bisulfite-Seq
library_construction_protocol: Micro-dissection of the hippocampal dentate gyrus Genomic DNA was isolated using the QIAamp DNA Micro Kit or the Allprep DNA/RNA Micro Kit (both QIAGEN) following the manufacturer's manuals. RRBS libraries were prepared using the Premium RRBS Kit (Diagenode) and purified twice using Agencourt AMPure XP beads (Beckman Coulter; 1X bead volume). Quality and concentration of RRBS libraries were determined using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical) and a fragment analyzer with capillary size of 33 cm (Advanced Analytical).