title: GSM5111206: Chromatin-associated total RNA Trp 3h; Mus musculus; RNA-Seq
library_construction_protocol: Trizol extraction protocol. For WCE RNA, 5x106 cells were resuspended in Trizol. For chromatin-associated RNAs, 15x106 cells were fractionated using the method published in Werner & Ruthenburg, 2015, and the chromatin fraction resuspended in Trizol. For chromatin-associated total RNA sample, rRNA was depleted and libraries were prepared for sequencing of intronic and exonic RNA. For PolyA+ samples, libraries were prepared by selecting polyA+ RNAs.