title: GSM5824557: MDMs uninfected Donor 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from uninfected or CHIKV-infected MDMs was obtained using Direct-zol ™ RNA Miniprep Plus (Zymo Research, Irvine, California) following the manufacturer's protocol. RNA samples were treated with DNase I column (Zymo Research, Irvine, California) to remove contaminating genomic DNA. RNA was quantifed, and the quality of each RNA sample was checked by spectrophotometry (Thermo Scientifc, Wilmington, DE, USA). Additionally, RNA quality was determined by analysis of ribosomal RNAs, using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Libary construction was made using the KAPA Stranded mRNA-Seq Kit (cat #KK8421, KAPA Biosystems, Wilmington, MA) RNA-seq poly A selected