title: GSM4829290: TKO_DR.4 RNAseq rep 4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was TRIzol-extracted (Cat. 10296010, Thermo Fisher Scientific) and further purified using the Direct-Zol RNA kit (Cat. R2052, Zymo Research) with DNaseI in-column treatment according to the manufacturer's instructions and quantified with Nanodrop (Thermo scientific). After RNA quality control with bioanalyzer Agilent 2100 (Agilent Technologies), Paired-end Poly(A)-enriched RNA libraries were prepared with the Ovation RNA-seq System V2 Kit (Cat. 7102-08, NuGEN; strand specific) from the Gladstone Genomic core. The mRNA-seq libraries were analyzed by Agilent Bioanalyzer and quantified using an Illumina Library Quantification Kit (Cat. KK4824, KAPA Biosystems). Libraries were prepared by the Gladstone Genomics Core (http://labs.gladstone.org/genomics/home). High-throughput sequencing was done using an Illumina HiSeq 2500 instrument (http://humangenetics.ucsf.edu/genomics-services/sample-processing/).