title: GSM6122190: db/db 12w; Mus musculus; RNA-Seq
library_construction_protocol: heart tissues were removed, flash frozen on dry ice, and RNA was harvested using TruSeq™RNA Sample Preparation Kits v2. 1)rRNA depleted and RNA fragmentation-Optional;2)Purify the mRNA and Fragmentation-Optional;3)Synthesize First Strand cDNA;4)Synthesize Second Strand cDNA;5)Adenylate 3' Ends;6)Ligate Adapter;7)Amplification;8)QC the Library.