title: Illumina HiSeq 2500 paired end sequencing; Sorted tumor RNA-seq of syngeneic murine mutant IDH1 ICC tumor model treated with IDH1 inhibitor against vehicle
design: Sorted tumor RNA-seq of syngeneic murine mutant IDH1 ICC tumor model treated with IDH1 inhibitor against vehicle
library_construction_protocol: Samples were from vehicle- or inhibitor-treated mice and sorted with Non-Tumor Cell Depletion Cocktail from Miltenyi RNA was extracted from homogenized samples using the Direct-zol RNA Miniprep Plus kit (ZYMO) in accordance with the manufacturer-prescribed protocol.  RNA sequencing (RNA-Seq) library preparation used the NEBNext Ultra Directional RNA Library Prep Kit for Illumina by following the manufacturers recommendations (NEB).