title: GSM5272917: Salmonella within Acanthamoeba replicate 2; Salmonella enterica subsp. enterica serovar Typhimurium str. ATCC 14028; RNA-Seq
library_construction_protocol: Total RNA was extracted with ExtractRNA reagent (Evrogen, Moscow, Russia). The RNA samples were treated with a TURBO DNA-free™ kit (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer's instructions. The RNA content was determined in a Qubit fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity, purity, and concentration were assessed with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). The Cappable-Seq procedure was performed as previously described [Ettwiller et al., 2016]. Cappable-Seq