title: Illumina HiSeq 4000 paired end sequencing; Effect of miR-106b~25 in mouse spermatogenesis and male fertility
design: Effect of miR-106b~25 in mouse spermatogenesis and male fertility
library_construction_protocol: miR-106b~25 null mutants and miR-17~92flox/flox control mice on a C57BL/6 x 129S4/SvJae genetic background, were obtained from the Jackson Laboratory (Bar Harbor, ME, USA; Stock No: 008460 and 008458, respectively).   To generate miR-106b~25-/-;miR-17~92+/- mutant mice, miR-17~92flox/flox mice were crossed to CAG-Cre mice and the resulting miR-17~92+/- offspring were crossed to miR-106b~25-/- mice to generate compound double heterozygotes. These latter mice were back-crossed to miR-106b~25-/- mice to produce miR-106b~25-/-;miR-17~92+/- mice. Mice were bred in our animal house facility at the Center of Biomedical Reseach of the University of Granada. Total RNA was isolated from three samples of every type of mouse testes: miR-17~92flox/flox control mice, miR-17~92+/-, miR-106b~25-/- and double mutant miR-17~92+/-; miR-106b~25-/-, using the Quiagen RNeasy Midi kit following the manufacturers instructions.  After successfully passing Macrogen Inc. quality check, libraries were prepared using TrueSeq RNA Sample Prep Kit V2 the RNAs were paired-end sequenced