title: GSM5425707: HuDko1; Mus musculus; miRNA-Seq
library_construction_protocol: Brains were removed and the straita were dissected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Total RNA was pretreated using NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, NEB, Ipswich, MA, USA) and RiboZero Magnetic Gold Kit (Human/Mouse/Rat) (Epicentre) b The treated RNA was used to prepare miRNA sequencing libraries with NEB Multiplex Small RNA Library Prep Set for Illumina. The DNA fragments in well mixed libraries were denatured with 0.1 M NaOH to generate single-stranded DNA molecules. These were then captured on Illumina flow cells, amplified in situ and sequenced for 51 cycles on an Illumina NextSeq 500 platorm (Illumina, Inc. San Diego, CA, USA).