title: GSM5272780: Col_H3K27me3_rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: For H3K27me3 antibody (ab6002, Abcam), the resin was washed with low salt wash buffer (150 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA pH 8.0, and 20 mM Tris-HCl pH 8.0), high salt wash buffer (500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA pH 8.0, and 20 mM Tris-HCl pH 8.0), LiCl wash buffer (0.25 M LiCl, 1% NP-40, 1% sodium doxycholate,1 mM EDTA pH 8.0, and 10 mM Trish-HCl pH 8.0), TE wash buffer (10 mM Tris-HCl pH 8.0 and 1 mM EDTA). Libraries were built for ChIP-seq (Illumina HiSeq X Ten; 150-bp pair-end sequencing)