title: GSM5979829: G17_20_Control; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted in TRIzol-Chloroform and purified using the RNeasy micro Kit (QIAGEN) including DNAse treatment. RNA quality and quantity were assessed by capillary electrophoresis using High Sensitivity RNA reagents with the Fragment Analyzer (Agilent Technologies) and RNA concentration was measured both by spectrophometry using Fragment Analyzer capillary electrophoresis. All RINs were higher than 9.0 and RNA concentration was between xx and yy per sample. An equimolar pool of the final indexed RNA-Seq libraries was prepared, using the NuQuant system (Nugen), and sequenced on a NovaSeq6000 from Illumina with paired-end reads of 100 bases and a mean sequencing depth of 50 millions per sample