title: Bisulfite sequencing of adult male whole bone marrow
design: Whole-genome bisulfite sequencing was performed using the EpiGnome/TruSeq DNA Methylation Kit following manufacturer protocols. Briefly, the DNA was denatured at 98C for 10 minutes followed by bisulfite conversion at 64C for 2.5 hours and DNA clean-up with the EZ DNA Methylation Gold Kit (Zymo Research). Library molecules are then generated via DNA synthesis of the converted DNA using random-primed synthesis, digestion of random primers, 3p tagging, purification using AMPure beads, and limited cycle amplification (10 cycles) to incorporate sample-specific index sequences.