title: GSM5311884: pSRKa; Agrobacterium fabrum str. C58; RNA-Seq
library_construction_protocol: Total RNA was prepared using Qiagen RNEasy kit, and contaminating DNA was removed with the Turbo RNAse-free DNAse kit for 1 hr at 37 ⁰C Integrity of the RNA was checked with Agilent Tapestation, samples with RIN >7 were processed using the following steps. 1ug of total RNA was used for ribosomal RNA depletion with the Illumina Ribo-Zero kit bacteria.  After rRNA depletion, the libraries were prepared following the Illumina TruSeq Stranded mRNA library preparation kit protocol. The cleaned adapter ligated libraries were pooled and loaded on the NextSeq 500 with a 75 cycle sequencing module to generate paired-end reads. Finally, the demultiplexing of the reads was performed with bcl2fastq version 2.20.0 pipeline.