title: WGS of homo sapiens: Sample RES03251
design: PCR-free, 150 bp, paired end, whole-genome sequencing was performed on a HiSeqX10 at TAGC. Samples were sheared using the Covaris LE220 (target peak size = 410bp). Sequencing libraries are generated from fragmented DNA using the Illumina TruSeq DNA PCR-Free HT Library Preparation Kit with minor modifications for automation (Hamilton STAR Liquid Handling System). Adapters for ligation used either TruSeq DNA CD Indexes or IDT for Illumina TruSeq DNA UD Indexes (96 Indexes, 96 Samples).  Library size distribution and absence of free adapters or adapter dimers was assessed by automated capillary gel-electrophoresis (Advanced Analytical Fragment Analyzer).  Library yield was determined by qPCR quantitation using the KAPA qPCR Quantification Kit (Roche Light Cycler 480 Instrument II). Normalization of libraries to 2.2 nM was performed into a working 96 well plate by automation (Hamilton STAR Liquid Handling System). Libraries were clustered as 8-plex pools or individually on an Illumina cBot2 using the HiSeq X PE Cluster Kit and a HiSeq X Flow Cell v2.5 before sequencing on an Illumina HiSeq X System with 151+7+151 cycle parameters using HISeq X HD SBS Kit reagents.