title: GSM5111700: S5: Heart, DAC; Sus scrofa; RNA-Seq
library_construction_protocol: Total RNAs from the ventricular tissues of Sham (n=3) and DAC (n=3) minipigs were isolated using TRIzol® Reagent(Thermo Fisher Scientific, USA). Each group contains three independent samples. NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) was used with 3 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols