title: GSM6170878: heads_FLAMseq_1; Drosophila melanogaster; OTHER
library_construction_protocol: For head transcriptomes, 3-day-old w1118 flies were collected and flash-frozen in liquid nitrogen and heads were used for RNA extraction. FLAM-seq libraries were prepared as described in (Legnini, I, et al., 2019) (extended protocol available at 10.21203/rs.2.10045/v1) using 4µg total RNA from 3-day-old w1118 fly heads and 14-16h AEL embryos. After SMRTbell adapter addition, libraries were sequenced on 3 SMRTcells on a Sequel I PacBio sequencer. Three replicates were performed for each tissue. Reads were processed using the FLAMAnalysis pipeline described in (Legnini, I, et al., 2019, https://github.com/rajewsky-lab/FLAMAnalysis)