title: GSM5112214: TR5: MCoV infected+ treated 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from right middle lung lobe using Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) in combination with a ball mill (Retsch) Sequencing libraries were be prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module and the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen).