title: Illumina HiSeq 2500 paired end sequencing
design: RNA-Seq of Arabidopsis thaliana wox135 triple mutant (Col-0 background).
library_construction_protocol: Total RNA was isolated RNeasy Plant Mini Kit. 700 ng total RNA has initially been used for polyA enrichment with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Subsequent library preparation has been performed with NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) according to the manufacturer's instructions. Quality and quantity were assessed at all steps via capillary electrophoresis (TapeStation, Agilent Technologies) and fluorometry (Qubit, Thermo Fisher Scientific). Libraries were immobilized and processed onto a flow cell with cBot (Illumina) and subsequently sequenced on HiSeq2500 system (Illumina) with 2 x 100 bp paired end reads.