title: RNA-seq of S cerevisiae tup1 with ScTUP1 added back replicate 2
design: RNA was purified using the Zymo Research Corp Direct-zol RNA Mini-Prep Plus kit and the concentration of RNA was determined using the Qubit 3.0 Fluorometer and the Qubit RNA HS Assay kit. The RNA library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina protocol from New England BioLabs, Inc. Samples were diluted in 0.1X TE to 4 nM and the Illumina NextGen MiSeq sequencer was used to sequence the samples.