title: GSM5008028: ATACSeq_ARID1BKD_iPSCErythroid_Hypoxia-rep3; Homo sapiens; ATAC-seq
library_construction_protocol: For ATAC-Seq, transposition reactions were performed on 50,000 non-CMS and non-CMS with ARID1B KD (replicates, n = 3) hiPSC-derived erythroid cells grown under hypoxic and normoxic conditions for two weeks as previously described Buenrostro et al., 2013 (52). Briefly Cells were washed with PBS followed by lysis with cold lysis buffer. Cells were then suspended in 50 μl 1X reaction buffer. Transposase reactions were carried out at 37°C for 30 minutes and DNA was purified using ChIP DNA Clean & Concentrator kits. DNA was amplified and library was generated using the dual barcoded primers (Illumina) using NEBNext High-Fidelity 2XPCR Master Mix for 14 cycles.