title: Illumina MiSeq paired end sequencing; rec-Y2H matrix in S. cerevisiae screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins
design: rec-Y2H matrix in S. cerevisiae screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins
library_construction_protocol: Yeast cells were harvest by centrifugation Yeast cells were grown in liquid gel media for 60 h at 30oC Yeast plasmid DNA was extracted using Zymoprep Yeast Plasmid Miniprep II kit Plasmid DNA was sheared by covaris, end repaired, recicularised by intramolecular ligation, and amplified using specific primers with R1/R2 linkers.