title: GSM5585897: Mcidas n3; Mus musculus; RNA-Seq
library_construction_protocol: cDNA libraries and RNA sequencing Library preparation and Illumina sequencing were performed at the Ecole normale superieure genomic core facility (Paris, France) 10 ng of total RNA were amplified and converted to cDNA using SMART-Seq v4 Ultra Low Input RNA kit (Clontech) Afterwards an average of 150 pg of amplified cDNA was used to prepare library following Nextera XT DNA kit (Illumina) Libraries were multiplexed by 12 on a high-output flow cells A 75 bp read sequencing was performed on a NextSeq 500 device (Illumina) A mean of 47 ± 9 million passing Illumina quality filter reads was obtained for each of the 12 samples