title: GSM5702363: rnaseq_control_ siRNA_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Life Technologies, Carlsbad, CA, USA). Total RNA (1 μg) was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, San Diego, CA, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA,  USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using  the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). These libraries were sequenced on an Illumina HiSeq 4000 sequencer (Illumina, USA) and 150 bp paired-end reads were generated according to the instructions of manufacturer.