title: GSM5655102: YCC10 H3K27ac; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq, sheared chromatin were subjected to immunoprecipitation with 2 μg antibody against H3K27ac (ab4729, Abcam), H3K4me1 (ab8895, Abcam) or 5 μg antibody against TEAD1 (610923, BD Biosciences). For RNA-seq, total RNA was extracted following manufacturer's protocols (Qiagen) For Nano histone ChIP-seq, ChIP and input DNA were subjected to whole-genome amplification using the WGA4 kit (Sigma-Aldrich) and a universal primer linked to BpmI restriction site (Qiagen). 30ng of BpmI-digested DNA was used for ChIP-seq library preparation according to manufacturer's protocols (New England Biolabs). For TF ChIP-seq, 30ng of ChIP and input DNA was used for library preparation. For RNA-seq, libraries were prepared accordingly using Tru-Seq Stranded Total RNA with Ribo-Zero Gold kit (Illumina), with an input of 1 μg total RNA.