title: GSM5653642: HCT116 200k cells Lb1 cTSA-seq input Rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were permeabilized with PBS +0.25% Triton X-100 and stained for Lamin B1, and then a secondary HRP antibody. THe TSA reaction was performed according the kit [Biotin-XX-Tyramide Superboost kit (Invitrogen, #2005939)]. The cells were then further solubilized in RIPA buffer and sonicated with a Bioruptor Pico prior to Streptavidin pulldown. Libraries were produced with the Illumina TruSeq RNA library kit v2 (for RNA) or the Rubicon Genomics (Takara) ThruPlex 48 kit.