title: GSM6284424: D3_Control_donor2; Homo sapiens; RNA-Seq
library_construction_protocol: 300 CD4+ EGFRt+ CAR-T cells were FACS-sorted into SMART-Seq HT lysis buffer (Takara). cDNA was generated using 13 amplification cycles and purified using AMPure XP beads (Agencourt). Libraries were prepared using the Nextera XT kit (Illumina) and sequenced in an SP flow cell on a NovaSeq 6000 (Illumina) to read depths >10x106 per sample.