title: GSM4830640: Mid 2-cell rep2; Mus musculus; RNA-Seq
library_construction_protocol: The RNA extraction was performed using the Smart-seq2 protocol as published with minor modification. Cells were lysed in 0.1% (vol/vol) Triton X-100 lysis containing 2.5µM of oligo-dT30VN primer, 2.5mM of dNTP mix and 1U/µl of RNase inhibitor. After 3 mins lysis at 72℃, the Smart-seq2 reverse transcription mixture was added for reverse transcription. cDNA was pre-amplified and AMPure XP beads were used for purification. Smart-seq2 protocol