title: GSM5701814: eWAT saline rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol (Invitrogen) PolyA mRNA librairies were prepared from 200ng of total RNA using a NEB E6420 kit, according to the manufacturer's recommendations. Quality of the libraries was evaluated on a Bionalyzer DNA HS Chip (Agilent) and quantified using a Qubit fluorimeter (Invitrogen). The libraries were then pooled and sequenced on Illumina Nextseq500, PE40