title: GSM5397927: PBS [Spatial transcriptomics]; Mus musculus; RNA-Seq
library_construction_protocol: WT mice were injected with either PBS or LCWE and one week later, heart tissues collected, embedded in OCT and sectioned on a cryostat at a thickness of 10µm. Tissue was enzymatically permeabelized accroding to the Visium protocol. RNA libraries were prepared for sequencing using the Visium Spatial 3' V1, 10x Genomics protocol. Sequencing was done with NovaSeq 6000 using a custom paired-end sequencing protocol, consisting of: read 1, 28 cycles; index 1, 10 cycles; index 2, 10 cycles; read 2, 120 cycles.