title: GSM5979972: Quadriceps wildtype C57BL10; Mus musculus; RNA-Seq
library_construction_protocol: Quadriceps were isolated and fresh-frozen in LN-cooled isopentane, sectioned on the cryostat in 10µm thick sections that were placed on the Visium slide (n=1 per mouse model), methanol fixation, H&E staining, imaging, after permeabilization, reverse transcription, second strand synthesis and denaturation occurred, hereafter cDNA was amplified and finally, to generate a sequencing ready indexed library, P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 were added via end repair, A-tailing, adaptor ligation and PCR amplification. The spatial gene expression libraries were sequenced on an Illumina NovaSeq6000 with a target of ~125 million Paired-End reads per sample, or 50,000 reads per spot for each sample. Libraries were prepared following the standard processing protocol of 10x Genomics, using a Dual Index Kit TT Set for Illumina sequencing Next generation whole transcriptome sequencing (RNA-seq)