title: GSM5311848: OVCAR3_siNMNAT-2_mNMNAT2 Polysome-rep2.fastq.gz; Homo sapiens; RNA-Seq
library_construction_protocol: Input RNA and polysome associated RNA was isolated using the RNeasy kit (Qiagen) according to the manufacturer's instructions from 2 individual 15 cm plates per replicate per treatment. The total RNA was then enriched for polyA+ RNA using Dynabeads Oligo(dT)25 (Invitrogen).  The polyA+ RNA was then used to generate RNA-seq libraries. The RNA-seq libraries were subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of final library DNA fragments) and sequenced using an Illumina Nextseq 500.