title: GSM4829688: mm_ko_rep1 [RNA-Seq]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from sorted BM populations using the RNeasy Micro Kit (QIAGEN) as described by the manufacturer. RNA was subjected to double-stranded cDNA synthesis using the Ovation RNA-Seq System V2 (NuGEN), cDNA libraries were prepared form sheared cDNA fragments (150-550 bp) using the Ovation Ultralow System V2 (NuGEN). The indexed cDNA libraries were pooled in equimolar ratios and subjected to 75-cycles of sequencing at Exiqon (Exiqon now at Quiagen, Aarhus, DK) on a Illumina NextSeq 500 System (Illumina, San Diego, CA, USA.