title: Illumina HiSeq 2500 paired end sequencing; Single cell five-prime end sequencing of PBMC - resting and stimulated
design: Single cell five-prime end sequencing of PBMC - resting and stimulated
library_construction_protocol: Human PBMCs were prepared from whole blood of a male healthy donor with Leucosep™ (Greiner®). Isolated 2×106 PBMC cells were incubated with PMA/ionomycin (i.e. stimulated) (Cell Activation Cocktail with Brefeldin A, Biolegend®), or DMSO as control (i.e. resting), for six hours. Freshly prepared resting and stimulated PBMCs were subjected to sc-end5-dT (Single Cell 5′ Library kit v1.1) and library construction on the same day using the Chromium™ platform according to manufacturer's instructions (10x Genomics®). About 5,000 cells/nuclei were targeted per reaction As per 10x Genomics Chromium platform (Single Cell 5′ Library kit v1.1)