title: RNA-Seq of Homo sapiens: Alzheimer's Disease Female Precuneus
design: RNA was extracted from 30 mg frozen tissue from the precuneus (PREC) or         primary visual cortex (VIC) using TRIzol (Life Technologies, Thermo Fisher Scientific,         Carlsbad, CA 92008, USA) including an on-column DNase I treatment. 500 ng of RNase H         ribodepleted total RNA was used for library preparation with the TruSeq Stranded Total RNA         Sample Prep Kit (Illumina, USA). Mixes of External RNA Controls Consortium (ERCC) Spike-Ins         (Thermo Fisher Scientific) with different molar ratios were added to control (Mix 1) and AD         samples (Mix 2). The libraries were sequenced using the Illumina HiSeq2500 at the Kinghorn         Centre for Clinical Genomics, Garvan Institute of Medical Research, Sydney,         Australia.