title: GSM4829776: littermate_control_input [chipseq_tg_wt_3m]; Mus musculus; ChIP-Seq
library_construction_protocol: CA1 region of hippocapus was surgically dissected under microscope, snap frozen in liquid nitrogen and stored at -80c until further use. NeuN+ neuronal nuclei were FACS sorted and used to perform chromatin immunoprecipitation (ChIP) for H3K4me3 Chromatin immunoprecipitation was performed according to (Halder et al., 2016) with 0.2 µg chromatin and 1µg H3K4me3 (ab8580) antibody. ChIPseq library preparation was performed using NEBNext Ultra II DNA library preparation according to manufacturer's protocol. 2nM libraries were pooled and sequenced in Illumina Hiseq 2000 with 50-bp single end reads.