title: GSM5702025: Quiescent Replicate 2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cultures were pelleted and resuspended in QIAzol (Qiagen). Cells were lysed using a 0.5 mm Zirconia/Silica beads (RPI #9834) in a Mini-Beadbeater high energy cell disruptor (BioSpec) at 4°C, for 4 times 60 seconds. Samples were cooled on ice for 2 min in-between beatings. RNA was subsequently purified using RNeasy Mini kit (Qiagen). 1 ug of RNA was used to construct sequencing libraries using the NEBNext Ultra II RNA library preparation kit for Illumina (New England Biolabs; NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers) and single-end sequenced (75 bp) on the NextSeq 550 platform (Illumina) in accordance with the manufacturer's protocol.