title: GSM5272783: control Huh7 cells_3; Homo sapiens; RNA-Seq
library_construction_protocol: Ectosomes were isolated by differential centrifugation processes. LX-2 cells were cultured in DMEM medium containing 10% FBS for 36 hour. Culture medium was first collected and centrifuged (300 g, 10 min, 4℃) to remove cells. The supernatant was then centrifuged (2,000 g, 20 min, 4℃) to remove cell debris. The collected medium was centrifuged again (16,500 g, 30 min, 4℃) to obtain pellets containing the ectosomes.Huh7 cells were preincubated with different ectosomes or not for 12 hours and then  fresh medium for another 12 hours. Lastly, RNA in different groups of Huh7 cells were extracted by Trizol for RNA-seq. RNA libraries were prepared for sequencing using standard Illumina protocols