title: GSM5273001: D1 scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh HNSCC tissues were dissociated with Gentle MACS Dissociator (Miltenyi Biotec) according to the manufacturer's instructions. Next, single cells were counted using an automated cell counter and adjusted to a concentration of 1000 cells/μL, and we retained single-cell transcriptomes for 13,903 cells after initial quality controls. Cellular suspensions were loaded on a 10X Genomics GemCode Single-cell instrument that generates single-cell Gel Bead-In-EMlusion (GEMs). The cells were lysed, and RNA was reverse-transcribed into cDNA. Libraries were generated from the cDNA with Chromium Next GEM Single Cell 3' Reagent Kits v3.1. the cDNA libraries were sequenced using the Nova 6000 Sequencing System (Illumina).