title: GSM6283986: Brca2KO1, DMSO; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Cells were lysed with 1 mL of TRIzol (Thermo Fisher Scientific, 15596026) and 200 µL of Chloroform following the manufacturer's instructions. The collected RNA was purified with PureLink RNA Mini Kit (Invitrogen, 12183018A) following the manufacturer's protocol. mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. mRNA was enriched using oligo(dT) beads, and rRNA was removed using the Ribo-Zero kit. The mRNA was fragmented, and cDNA was synthesized by using an mRNA template and random hexamers primer, after which a second-strand synthesis buffer (Illumina), dNTPs, RNase H, and DNA polymerase I were added for the second-strand synthesis, followed by adaptor ligation and size selection. The library was sequenced by the Illumina Novaseq platform.