title: GSM5916407: Math1-Cre_SmoM2_Piezo2KO_MB_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Math1-Cre; SmoM2 medulloblastoma and Math1-Cre; SmoM2; Piezo2fl/fl medulloblastoma from P21 mice were harvested followed by mechanical and enzymatic dissociation using the Papain Dissociation System (Worthington Biochemical Corporation). Single cell suspension was assessed by Trypan blue. Approximately 5,000 cells at a concentration of ~1,000 cells/μl were loaded onto the Chromium Controller. Library generation for 10X Genomics v3 chemistry was performed using the Chromium Single Cell 3ʹ Reagents Kit. Quantifications of cDNA libraries were performed using the Kapa Library Quantification Illumina/ABI Prism Kit (KAPA Biosystems) and the high-sensitivity DNA chips. 10X libraries were sequenced using the Illumina 2500 sequencing platform to achieve ~25,000 reads per cell. Two biological replicates of Math1-Cre; SmoM2 medulloblastoma. Two biological replicates of Math1-Cre; SmoM2; Piezo2fl/fl medulloblastoma. Four libraries in total.