title: GSM6284514: MiaPaCa2, injected Part2, Leader, rep3 [s_Part2Lead3]; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Micro Kit (Qiagen #74004). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2 ng total RNA with RNA integrity numbers ranging from 9.5 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a PE50 run, using the HiSeq 3000/4000 SBS Kit (Illumina).  An average of 34 million paired reads was generated per sample and the percent of mRNA bases per sample ranged from 63% to 83%.