title: GSM6118770: Galdos_Seq_Run3; Homo sapiens; RNA-Seq
library_construction_protocol: Droplet based extraction: HiPSC-derived cardiac differentiation cells were dissociated into single cell suspensions. Individual samples per timepoint and cell line were labeled using chemically modified lipid oligos using the 10X Genomics CellPlex kit. Single cells  were subsequently captured and lysed using 10X Genomics Chromium Next GEM Single Cell 3' v3.1 Kit with CellPlex. mRNA was reverse transcribed to cDNA, and cDNA pre-amplification was conducted following manufacturer's protocol. Droplet based library construction: Libraries for samples processed using 10X Genomics Single Cell 3' v3.1 with CellPlex kit were prepared from pre-amplified cDNA and CellPlex libraries according to manufactrer's protocol. Gene expression libraries were generated for all 10X samples, and CellPlex multiplexing libraries were generated for each individual single cell run. Individual library index primers were used for multiplexing libraries on flow cell during sequencing.