title: intestine in Oreochromis niloticus infected by S. iniae
design: We used NEB Next Ultra RNA Library Prep Kit(NEB, USA) to create cDNA libraries. First Strand Synthesis Reaction Buffer, random Primers and reverse transcriptase (Invitrogen, China) was used to synthesize first-strand cDNA. The second-strand cDNA was synthesized and purified, then end repair and adaptor ligatio of the adaptor. Qubit 2.0 was used to quantified the chosen products enriched by PCR amplification. Sequencing was performed using the Illumina HiSeqTM 2000, and the sequence data were obtained by Illumina Pipeline Software v1.6.