title: Illumina HiSeq 2500 sequencing; miRNA differential expression according to host-plant diet in two different Ostrinia species
design: miRNA differential expression according to host-plant diet in two different Ostrinia species
library_construction_protocol: Fertile egg masses of the two moth species EBB and Os were used to infest maize and mugwort plants in outdoor conditions in large cages.These egg masses were obtained after lab rearing of insects collected in the field close to Versailles (48°48′19″N, 2°08′06″E, France) in 2013. Development of the larvae was followed for about one month until the larval stage L4 was reached, based on the size of the head capsule. Larvae were collected and frozen at -80°C before sRNA extraction. sRNA extraction was performed on a pool of 15 L4 On or Os larvae per condition using the mirVana miRNA Isolation Kit (Ambion) according to the manufacturer's instructions. Extracted small RNA was quantified using a NanoDrop 2000 spectrophotometer (Thermo Scientific) and analyzed by PAGE and silver-staining. For construction of the libraries, sRNA were size-selected in the range of 15 to 40 nucleotides.