title: GSM5825101: S9: A549_GFP_None_3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted and on-column treated with DNase I with Quick-RNA Mini Prep kit (Zymo Research). Total RNA concentration and purity were measured by Nanodrop. RNA integrity was verified on 1% agarose gel and by RNA Screen Tape assay (Agilent) Messenger RNA (mRNA) was enriched from 1 μg of total RNA by Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer's instructions. cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer's protocol. Library concentration was measured by DNA High Sensitivity Kit (Invitrogen) on a Qubit fluorometer (Invitrogen). The High Sensitivity D1000 ScreenTape assay combined with the 2200 TapeStation system (Agilent) was used to assess the quality of the libraries.