title: bioluminescent Cladorhizidae sp. PacBio Iso-Seq FLNC
design: Total RNA was isolated from 25 mg of frozen sponge tissue using TRIzol reagent (Rio et al. 2010) at Brigham Young University. An Iso-Seq library was then prepared at Brigham Young University with the Iso-Seq Express Library Preparation Using SMRTbell Express Template Prep Kit 2.0 kit. This library was sequenced on a single Sequel II SMRT cell, and yielded 3.36 million CCS reads with a mean read length of 2.5 kb. Like the Bolinopsis sample, the primers and barcodes were removed with lima v2.2.0, and FLNC reads were generated with isoseq3 v3.4.0. There were 2.13 M reads remaining after barcode removal and FLNC processing.