title: GSM6118867: mESC_Firre_OE_control_5760min_dox_long_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol lysis reagent (Thermo Fisher) and purified using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. RNA concentration was measured with a Qubit fluorometer (Invitrogen), and RNA quality was determined using a Bioanalyzer or TapeStation (Agilent Technologies). RNA-seq libraries were prepared from 1 μg of total RNA using the TruSeq RNA Library Prep Kit v2 (Illumina) with poly(A) selection according to the manufacturer's instructions. Multiples of 12 randomized samples were each multiplexed, pooled, and run in one HiSeq lane.