title: GSM5587075: Acan-Cre+/Cre;Stat3fl/fl; Mus musculus; RNA-Seq
library_construction_protocol: Single cell samples were prepared using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 and chip Kit (10X Genomics) according to the manufacturer's protocol. Briefly samples were FACS sorted using DAPI to select live cells followed by resuspension in 0.04% BSA-PBS. Nearly 1200 cells/µl were added to each well of the chip with a target cell recovery estimate of 10,000 cells. Thereafter Gel bead-in Emulsions (GEMs) were generated using GemCode Single-Cell Instrument. GEMs were reverse transcribed, droplets were broken and single stranded cDNA was isolated. cDNAs were cleaned up with DynaBeads and amplified. Finally, cDNAs were ligated with adapters, post-ligation products were amplified, cleaned-up with SPRIselect and purified libraries were sequenced on Novaseq at the UCLA Technology Center for Genomics and Bioinformatics. Single Cell 3' Library & Gel Bead Kit v3.1