title: GSM5981806: HiC_UT242; Homo sapiens; Hi-C
library_construction_protocol: Hi-C libraries were prepared based on the in situ Hi-C protocol 16 with minor modifications. Briefly, 5 x 106 cells were cross-linked with 1% formaldehyde (Sigma #252549) for 10 min with gentle rotation and quenched with 125 mM glycine. Cells were washed twice with ice-cold PBS and cells were snap-frozen on dry ice for 30 min. Cell pellets were resuspended in 1 mL ice-cold cell lysis buffer and rotated for 30 min at 4°C.