title: GSM5469135: Endo_B_cell_sample_3; Homo sapiens; RNA-Seq
library_construction_protocol: Immediately after sorting, cells were pelleted by centrifugation and re-suspended in RLT  buffer (Qiagen, USA) and RNA was extracted using QIAGEN RNeasy Micro Kits (Qiagen,  USA) according to the manufacturer's protocol 'Purification of Total RNA from Animal  and Human Cells'. The procedure enriches for mRNA; all RNA molecules longer than  200 nucleotides are purified. Samples were treated as single cells using Smart-Seq2  protocol for cDNA conversion. The library was prepared using Standard  Nextera Illumina Library Prep kit according to manufacturer's protocol.