title: RNA-seq of homo sapien: adult monocytes: Sample PDBJ105NWQ_19_S46_L006
design: RNA sequencing library preparation was conducted using the NEBNext Ultra RNA Library Prep Kit for Illumina by following manufacturer's recommendations. Briefly, mRNAs were first enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 15 minutes at 94°C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapter was ligated to cDNA fragments, followed by index addition and library enrichment with limited cycle PCR.