title: Illumina HiScanSQ paired end sequencing; RNAseq of Huh7 and Huh7.5 cells treated with interferon alpha, beta or lambda.
design: RNAseq of Huh7 and Huh7.5 cells treated with interferon alpha, beta or lambda.
library_construction_protocol: Huh7 and Huh7.5 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin-streptomycin and 2mM L-glutamine, then treated for 8h with IFNs (or mock treated) before collection.  Cells were treated for 8h with 10ng/ml of Interferon-α2A (cod.H6041, SIGMA), 5000 U/ml of Interferon-β1A (cod.I4151, SIGMA) or 100ng/ml of interferon-λ3 (cod.E92028Hu, Uscn Life Science Inc) prior RNA extraction and library preparation. Total RNA was extracted with mirVana miRNA isolation kit and DNAse digested (Ambion) according to manufacturer's instruction. Libraries for Illumina sequencing were constructed by polyA selection with the Illumina Kit.