title: GSM5111159: Ischemic Stroke (IS); Mus musculus; RNA-Seq
library_construction_protocol: Single-cell gel beads in emulsions (GEMs) were generated by loading single-cell suspensions onto a Chromium Single-Cell Controller Instrument (10 × Genomics). Approximately 12,000 cells were added to each channel. After that, reverse transcription reactions were engaged to generate barcoded full-length cDNA, and cDNA clean-up was performed with DynaBeads Myone Silane Beads (Thermo Fisher Scientific). Next, cDNA was amplified by PCR and the amplified cDNA was fragmented, end-repaired, A-tailed, and ligated to an index adaptor, and then the library was amplified. Every library was sequenced on a HiSeq X Ten platform (Illumina), and 150 bp paired-end reads were generated. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.