title: GSM5701557: Rep1_Sample_R21055372-Over-IP-Over-IP; Mus musculus; MeDIP-Seq
library_construction_protocol: MeDIP experiments were performed using a MagMEDIP kit (Diagenode, Seraing, Belgium). Briefly, genomic DNA (gDNA) was extracted from FGSCs and sonicated to shear chromatin. Fifty nanograms of fragmented genomic DNA was used for the library construction and purified. Methylated DNA was immunoprecipitated with anti-5-methylcytosine antibody overnight at 4℃. After washing and elution, immunoprecipitated DNA was amplified and subjected to Illumina sequencing. Library was prepared with NEBNext Ultra End-Repair/dA-Tailing Module (E7442, NEB) and NEBNext Ultra Ligation Module (E7445, NEB). High-throughput sequencing of the ChIP fragments was performed using Illumina NextSeq 500 following the manufacturer's protocols.