title: GSM5233253: H4K5bu-A1; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The libraries were constructed following NEB® UltraTM II DNA Library Prep Kit for Illumina (E7645s). Briefly, ChIP DNA was end-blunted and added with an 'A' base so adaptors from the NEBNext Adaptor for Illumina with a 'T' can ligate on the ends. Then 200–400 bp fragments are beads-isolated and purified. The library was amplified by 15 cycles of PCR.