title: GSM4830721: Doe_Red_R1; Daphnia pulicaria; RNA-Seq
library_construction_protocol: Thirty animals from each of the four lakes were added directly to 8 mL of RNALater solution. Total RNA was extracted with Trizol Reagent (Thermo Fisher) following the instruction. The RNA samples were then purified with NEBNext rRNA Depletion kit (NEB E6310). The libraries were constructed using the NEBNext UltraII Directional RNA Library Prep kit for Illumina (NEB E7760) and the NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335).  