title: GSM5272646: wild type E. coli - P1vir infection time 10 min - rep. 1; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
library_construction_protocol: RNA isolation was performed using RNeasy Mini Kit (Qiagen) according the manufac-turer's protocol. The elution step was performed twice using 50 µl of RNase-free water on the same column to increase RNA yields. The RNA preparation was digested with DNase using TURBO DNA-free™ Kit (Ambion), according the manufacturer's protocol. Samples were stored at −80°C. The purity and integrity of RNA was assessed using the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and Agilent RNA 6000 Nano Kit, according the manufacturer's protocol. RNA depleted of ribosomal RNA with Ribo-Zero rRNA removal Kit Bacteria (Illumina). Library prepared with TruSeq Stranded Total RNA Sample Prep Kit (Illumina).