title: GSM5655253: Scrambled_siRNA_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the scrambled siRNA knockdown and the CUX1 siRNA knockdown ECs at passage 10 using an RNeasy Mini kit (Qiagen Cat#74104). All samples were quantified and assayed to confirm minimum RINs of at least 9.3 using an Agilent Bioanalyzer (High Sensitivity DNA Kit, Cat#5067-4626). 1 µg total RNA per sample underwent mRNA capture and was then fragmented at 94 ºC for 6 min. Sequencing library was prepared according to the manufacturer's protocol using 10 cycles of final amplification (KAPA mRNA HyperPrep Kit, Cat#KK8580 and KAPA UDI Adapter Kit, Cat#KK8727). Next generation sequencing was performed on Illumina NextSeq500 (75 bp paired end) to a targeted depth of ~20M reads/sample.