title: GSM5824072: OS cell lines-143B NC1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of Cells was isolated using the Trizol Reagent (Invitrogen Life Technologies) and quantified in a NanoDrop (ThermoFisher). 3 μg of total RNA were used for the RNA sample preparations. Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA). Products were purified (AMPure XP system) and quantified using the Agilent high sensitivity DNA assay on a Bioanalyzer 2100 system (Agilent). The sequencing library was sequenced on a Hiseq platform (Illumina) by Shanghai Personal Biotechnology Cp. Ltd. Sequencing libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).