title: GSM5848427: LM2 pool of 10 WBC at 10h from mouse 1000_2; Homo sapiens; RNA-Seq
library_construction_protocol: Using the CellCelector, an automated single-cell picking system (ALS), single CTCs, CTC clusters and CTC-WBC clusters were collected and immediately transferred into individual tubes (Axygen, 321-032-501) containing 2.5 μl RLT Plus lysis buffer and 1U SUPERase IN RNAse inhibitor (Invitrogen, AM2694). Samples were immediately frozen and kept at 800C until further processing. cDNA was amplifiead and libraries prepared according to the Smart-seq2 protocol.