title: RNA-seq of mus musculus embryo : Sephs1-/- at E7.5
design: Embryos were dissected from uterus at E6.5, E7.5 and E8.5 with the aid of super-fine forceps and a 29-gauge needle. RNA was extracted from whole embryos using TrizolTM reagentwith glycogen carrier.  DNase I was treated to remove genomic DNA (gDNA) following the manufacturers instruction. mRNA quality was assessed using the RNA 6000 Nano-Assay on a BioAnalyser 2100.