title: WGS of Ctena orbiculata gill
design: Nucleic acids from gill and foot tissue were extracted by using Qiagen DNeasy Blood & Tissue kits, following manufacturer instructions. Metagenome libraries were prepared by using 50 ng of DNA with an Illumina Nextera DNA sample preparation kit, and adapter sequence additions (using 50 cycle PCR) and fragmentation were done simultaneously. Initial and final DNA concentrations were determined with Qubit dsDNA HS Assay kit (Life Technologies). Library sizes were measured using an Agilent 2100 Bioanalyzer, and libraries were clustered with Illumina cBot prior to paired-end sequencing for 300 cycles on an Illumina Hi Seq 2500 system