title: mtDNAmut patient-PLC005-dose-8.8 (fig2d)
design: Total DNA was processed with REPLI-g mitochondrial DNA kit (QIAGEN, CAT#151023) according to manufacturer instructions from roughly 10ng DNA. Resulting material was cleaned with 0.5X ratio SPRI Beads (AMPure XP, Beckman Coulter) analyzed by Agilent Tapestation on genomic DNA tape. 100 ng input DNA was sheared using Covaris E220 Focused-ultrasonicator (Covaris) with the following 350 bp insert setting: duty factor 10%, peak power 175W, cycles/burst 200, duration: 50 sec, temperature 5-10 degrees Celsius. Sequencing libraries were prepared using TruSeq DNA Nano Library Prep Kit (Illumina) according to manufacturer's protocol. Libraries were sequenced on Illumina Miseq sequencer with 150 bp paired ends read length, with a minimal coverage of 2500X for mitochondrial sequence.