title: GSM5468137: D75 RB1-Mut hESC (C1)-hRBOs rep2 RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA for each sample was isolated with TRIzol reagent and purified using RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions, RNA quality and quantity were assessed using NanoDrop 2000, Agilent 2100 Bioanalyzer and Agilent RNA 6000 Nano Kit. RNA library construction and RNA sequencing were performed by the annoroad Gene Technology. Sequencing libraries were generated using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB) and library clustering was performed using HiSeq PE Cluster Kit v4-cBot-HS (Illumina) following the manufacturer's recommendations. After cluster generation, the libraries were sequenced on an Illumina platform and 150 bp paired-end reads were generated.