title: GSM5653670: Cell from adult ovary follicle, S_L5; Homo sapiens; RNA-Seq
library_construction_protocol: For single-cell RNA-sequencing, the small antral follicles (1-8 mm) were collected in saline solution (0.9% NaCl, Fresenius Kabi, Bad Homburg, Germany). The follicles were mechanically ruptured with tungsten needles and the region with the cumulus GCs and oocyte was trimmed and transferred into a clean dish. Individual follicular cells were manually picked up using a pulled glass-capillary under a stereo microscope (Zeiss). Oocytes were denuded by pipetting up and down. None of the collected oocytes contained a polar body. Each cell was transferred into a cold PCR tube contain 1.9 ml 0.2% TritonX-100 in DEPC water and 0.1 ml Recombinant RNase inhibitor (2313A, 40U/ml, TaKaRa), snap-frozen on dry ice and stored at -80°C. Sequencing libraries were prepared according to the user guide of the Nextera XT (illumina)