title: RNA-Seq of Aspergillus loretoensis under two salinities
design: Libraries were constructed with the TruSeq RNASeq v2 Set A Kit (Illumina, San Diego, CA, USA), and the TruSeq RNA Seq Sample Prep v2 Guide (Illumina, Part 15026495 Rev F). The libraries concentration was quantified with a Qubit 3.0 using the Qubit dsDNA Broad Range (Life Technologies, USA) assay and cDNA fragments population were checked in a 4% agarose gel. The final average size of each library was determined on an Agilent 2100 Bioanalyzer using the DNA 1000 kit (Agilent Technologies, Germany). The final concentration of libraries and sequencing control (PhiX) was 10 pM and 1%, respectively. The Illumina MiSeq platform and the MiSeq System Reagent V3 kit were used for sequencing, generating pair-end reads of 76 bp.