title: GSM4829601: MM126_S23_L006_R1_001; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA extraction, cells were resuspended in TRI Reagent (MRC) and then Direct-zol RNA MiniPrep (Zymo Research) was used for the RNA extraction. RNA was quantified using Nanodrop (Thermo Fisher Scientific), ethanol-precipitated, and stored in liquid nitrogen until sequencing. For library preparation, RNA Integrity Number (RIN) was measured using Agilent 2100 bioanalyzer. Agilent RNA 6000 Nano or Qubit RNA Assay Kits were used to measure RNA concentration. For depletion of ribosomal RNA, we used KAPA RNA Hyper with RiboErase Kit (KAPA Biosystems). Different adaptors were used for multiplexing samples in one sequencing run. Library concentrations and quality were measured using Qubit ds DNA HS Assay kit (Life Technologies) and Agilent Tapestation (Agilent).