title: GSM5701776: LIN37_KO_Strep-LIN9_Arrested_5M_Rep1_INPUT; Homo sapiens; OTHER
library_construction_protocol: Harvested cells were lysed in a buffer containing 50mM TrispH8.0, 150mMNaCl, 1%TritonX-100, 0.1%​sodium deoxycholate, 5mM CaCl2 and subjected to MNase-digestion. Digested input samples were subjected to RNase (37C) and Proteinase-K treatment at (65C o/n) prior to DNA purification by SPRI beads. For the Strep-precipitated samples, digested chromatin was bound to StrepTactin-XT beads (IBA-Lifesciences) , washed in RIPA buffers and the bead suspension was resuspended in a buffer containing 10 mM Tris pH 8.0, 5 mM EDTA, 300 mM NaCl, 0.6% SDS prior to RNase and Proteinase-K treatment as above. NEB Next Ultra II Library Prep Protocol MNase-ChIP seq