title: GSM5653455: 5_3; Takifugu alboplumbeus; RNA-Seq
library_construction_protocol: Total RNA of ventral part of telenchephalon, hypothalamus and pituitary were isolated with elimination of DNA contamination using DNase. mRNA was first purified from the total RNA using poly-T oligo-attached magnetic beads and then fragmented, after which cDNA synthesis was performed. Then the synthesized cDNA was used for the preparation of PE100 strand specific sequencing libraries. Strand-specific RNA-seq