title: GSM5825240: Lung treated with IFN-lammda2 for 1 day sample.9; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted with the RNeasy Mini Kit (QIAGEN). cDNA libraries were constructed from 10 ng of total RNA from lung tissues of mice. Library construction was performed using 100 ng of cDNA undergoing end repair, A tailing, ligation of universal TruSeq adapters and amplification of 8 cycles to incorporate unique dual index sequences. Libraries were sequenced on the NovaSeq 6000 (Illumina, San Diego, CA) targeting 40 million read pairs and extending 150 cycles with paired end reads.