title: GSM5111092: batch39; Mus musculus; RNA-Seq
library_construction_protocol: Sequencing libraries were prepared following a modified single-cell tagged reverse  transcription (STRT) protocol as previously reported (Li et al., 2017; Picelli et al.,  2013). The cells were first judged by morphology to assess their state, with cells in  good condition being picked and directly placed into lysis buffer. The reverse  transcription reaction was performed using a sample-specific 25-nucleotide (nt) oligo  dT primer containing an 8-nt barcode (TCAGACGTGTGCTCTTCCGATCTXXXXXXXX-  DDDDDDDD-T25, where X represents the sample-specific barcode,  and D stands for the unique molecular identifier (UMI)). After reverse transcription and  second-strand cDNA synthesis, the cDNAs were amplified by 16 cycles of PCR. The barcoded DNAs were then pooled together and purified using AMPure XP beads.  Biotinylated pre-indexed primers were used to further amplify the PCR product by an  additional four cycles of PCR to introduce biotin tags to the 3′ ends of the amplified  cDNAs. Approximately 300 ng cDNA was sonicated into 300-bp fragments using the  Covaris S2 system and enriched with Dynabeads MyOneTM Streptavidin C1 beads.  Libraries were constructed using a Kapa Hyper Prep Kit (Kapa Biosystems) and were  then submitted to150-bp paired-end sequencing on an Illumina Hiseq X Ten  platform(Novogene).