title: GSM5111196: Thoracic_M_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Spinal cords were rapidly extruded from reporter mice and subdissected by spinal cord level. Tissue was homogenized and nuclei were isolated (see protocol) prior to FACS sorting for GFP+ nuclei. Samples were processed on a Sony SH800 Cell Sorter with a 100 mm sorting chip and GFP+/DRAQ5+ nuclei were collected. Libraries were constructed according to the manufacturer's instructions (10X Genomics).