title: GSM5008288: rat_NPC_Day8_Rep1_ATAC; Rattus norvegicus; ATAC-seq
library_construction_protocol: Approximately 50,000 embryonic stem cells and differentiated cells were collected for ATAC-seq. ATAC-seq was performed as previously described (Corces, M., Trevino, A., Hamilton, E. et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14, 959–962 (2017). https://doi.org/10.1038/nmeth.4396) with one amendment. DNA size exclusion was utilized Cells were lifted using Accutase. Total RNA was extracted by using the RNeasy kit (QIAGEN). DNA was extracted by using omni ATAC protocol previously described