title: GSM6250322: MPTP mouse SNpc, 14 days treatment, treated [SO-4248-T3]; Mus musculus; RNA-Seq
library_construction_protocol: All the reagents used for RNA isolation and cDNA synthesis were prepared in RNase free conditions using DEPC water. RNA from mouse brain tissue was isolated using TRIzol reagent (Invitrogen Cat# 15596018) and bromo-chlorophenol (BCP) (Molecular Research Centre, Inc. Cat# BP151). RNA was isolated from human autopsy brain tissue using the RNeasy Plus Universal Mini Kit (Qiagen Cat# 73404). RNA libraries for RNA-seq for mouse RNA were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB #E7420G, 30061409) following manufacturer's protocols. RNA-seq library preparation for human tissue samples was performed using the TruSeq RNA Library Prep Kit v2 (Catalog IDs: RS-122-2001, RS-122-2002).