title: 16S rRNA amplicon sequencing of plastic immerged in marine aquarium
design: DNA extraction was performed with DNeasy PowerSoil Pro Kit ; the first PCR was used with the primers : 806R (5-GGACTACNNGG GTATCTAAT-3) and 341F (5-CCTAYGGGRBGCASCAG-3); an additional PCR was carried out with the same primers that contained adaptor barcode tags and spacers.