title: GSM4830165: DU145_2_rep2; Homo sapiens; OTHER
library_construction_protocol: Polymerase chain reaction (PCR) was applied to identify the successful transfection of lentiCRISPRa vector in DU145 cells. CRISPRa-F: TCTTGTGGAAAGGACGAAACACCG and CRISPRa-R: CTCCTTTCAAGACCTAGGATC were selected for PCR amplification region (209 bp) from lentiCRISPRa vector, followed by electrophoresis. The thermocycling parameters of PCR were 95℃ for 60 s, 35 cycles of (95℃for 10 s, 56℃ for 10 s, 72℃ for 30 s) and 72℃ for 1 min. libraries were prepared for sequencing using standard Illumina protocols