title: GSM5233276: fetal Leydig cells S1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were enzymatically dissociated with Trysine-EDTA and then filtered (70µm). The single cell solution is then sorted by fluorescent-active cell sorting to obtain a solution of alive and single Nr5a1-GFP+ cells. RNA was extracted from these cells with RNeasy Mini kit (Qiagen). Sequencing libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold (Ribo-Zero), with 3 replicates by condition. Sequencing with Illumina HiSeq 2500 (50 bp, paired-end)