title: GSM5915422: Mouse granulocytic and monocytic progenitors for H3K27me3 ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: Briefly, one million cells were fixed with 1% formaldehyde and sonicated with Covaris E220 or Bioruptor Plus. Sonicated chromatin was then incubated with antibody against H3K27me3(Cell Signaling, #9733), H3K27ac (Diagenode, #C15410196) overnight. Protein A/G beads (ThermoFisher) were added to the sonicated chromatin for 2 hours. After washes with low-salt, high-salt and LiCl wash buffer. Following washes, beads were de-crosslinked with Proteinase K (Zymo Research) at 55 °C overnight. Pull-downed DNA was extracted by DNA clean and concentration kit (Zymo Research). Accel-NGS® 1S Plus  DNA Library Swiftbio Science (IDT XGEN DNA ssDNA low input since 2022 )