title: GSM5273203: tumor endothelial cells - Rat IgG treated -1; Mus musculus; RNA-Seq
library_construction_protocol: Tumor tissues were minced and digested with 25ug/ml Liberase to obtain the single cell suspension. Tumor endothelial cells were isolated using FACS sorting (CD31+CD45- population). Total RNA was extracted and purified using QIAGEN RNeasy Mini Kit (Cat.74104) from tumor endothelial cells. The mRNA libraries were constructed using Takara SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Cat. 634411). 150bp paired-end RNA sequencing was performed on Illumina NovaSEQ 6000 platform and the RNA-seq data were generated by the Genomics and Microarray Shared Resource at UCCC.