title: WGS of BARI PIAZ -1 from BARI, Bogura of Bangladesh
design: Samples from onion bulb, plant leaves and roots were used for total genomic DNA extraction. Two grams of tissue from young and rapid-growing onion plants (collected from Shibganj Bogura Spices Research Center, Bangladesh Agricultural Research Institute (BARI) Bangladesh (Longitude / Latitude- 251N 8919E) and pooled for DNA isolation according to the protocol described by commercial kit. Total genomic DNA from plant leaves/stem/ roots/flower/ fruit were isolated using commercial DNA extraction kit [AddPrep Genomic DNA extraction kit (AddBio, Korea)] and the quality, quantity of the DNA were evaluated. The quality and quantity of the DNA will be evaluated by the Qubit fluorometer (Invitrogen, Carlsbad, CA, USA). Purified DNA was sent for library preparation and been sequenced through commercial suppliers. DNA was sequenced using BGISEQ DNBSeq platform from BGI, China.