title: GSM4890151: Phc1-KO 4C rep3; Mus musculus; OTHER
library_construction_protocol: Washed the cells with PBS and removed supernatant. Then the pellet was resuspended with lysis buffer. The chromatin was digested with 200 units DpnII after pre-treatment with SDS and triton X-100. The reaction was heat-inactivated and then incubated with ligation buffer. Chromatin was reverse crosslinked after Proteinase K and SDS treatment. The DNA was then purified by DNA clean beads followed by the second digestion with NlaIII and ligation in ligation buffer. DNA was precipitated with acetate and ethanol. The precipitation was purified again by DNA clean beads. Amplified 4C products by PCR and paired-end libraries were prepared according to Illumina's instructions.