title: Illumina HiSeq 2500 sequencing; sRNA sequencing of TRV-infected Nicotiana benthamiana 16c
design: sRNA sequencing of TRV-infected Nicotiana benthamiana 16c
library_construction_protocol: Samples were collected from upper systemic leaves of TRV infected plants at one week post infection and three weeks post infection. The collected samples were flash frozen in liquid nitrogen. Plants were grown in Levington F2+S professional growth compost in a controlled growth chamber (SANYO/Panasonic) at 22°C with 16-h light and 8-h dark periods. Total nucleic acid (TNA) extracts were prepared from leaf tissue by phenol-chloroform extraction as described in White and Kaper, 1989.  Small RNA libraries were prepared from 1 μg of TNA using the Illumina TruSeq Small RNA Library Prep Kit and sequenced on the Illumina HiSeq 2500 platform with 50-base single end reads at Edinburgh Genomics (Edinburgh, UK).