title: GSM4829240: SC-His+ATc Rep 2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: To extract the plasmids, 50 OD660 of cells were pooled and resuspended in 2 ml SE buffer (0.9M sorbitol, 0.1M EDTA pH 8.0), 200 µl Zymolyase 100T (2 mg/ml) and 2 µl b-mercaptoethanol and incubated at 37°C for one hour, followed by standard mini prep extraction per manufacturer's instructions (Promega). The library spacers were amplified and extended as described by Smith et al. 2016. To generate the library, amPL43 was maxiprepped (Qiagen, Hilden, Germany) and cut with NotI. Gibson Assembly was used to clone the oligos in the NotI site of amPL43. The library was transformed into DH5a E. coli (NEB C2987H). Other: DNA amplicon sequencing