title: GSM6283870: spontaneously hypertensive rats, treated with angiotensin receptor-neprilysin inhibitor (ARNI) [ARNI_A_2]; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the rat tissues using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.1.3 ug of total RNA was used for the construction of sequencing libraries. The final library was generated by heated denaturing and circularizing the above double stranded PCR products using the splint oligo sequence, then followed by formatting the single strand circle DNA (ssCir DNA). DNA nanoballs (DNBs) were generated by library amplification and loaded into the patterned nanoarray and single end 50 bases reads on BGIseq500 platform (BGI-Shenzhen, China)