title: GSM5111777: Lung - 649d - ind_13; Mus musculus; RNA-Seq
library_construction_protocol: RNA sequencing was performed as previously described (Liu et al. 2016)  with slight modifications. Total RNA was extracted using Trizol reagent (Invitrogen). For sequencing library construction, all samples were randomized first and prepared with TruSeq RNA Sample Preparation Kit (Illumina) according to the manufacturer's instruction. Libraries were sequenced on the Illumina Hi-seq 4000 system in three lanes within one flow-cell, using the 150-bp paired-end module