title: bioluminescent Cladorhizidae sp. HiC - MluCI Library DS349 1 of 2
design: We generated one DpnII-based Hi-C library and one MluCI-based Hi-C library of the cladorhizid sponge using the same protocol used for Bolinopsis and for the unicellular outgroup-to-animal species (Adams et al. 2020). 388 ng of DNA of cell debris/chromatin into the digestion reaction. Concentration after ProtK was 14.4ng/uL in 30uL. Used 374ng into FS reaction. 25 minute FS reaction. Bound to 25 uL C1 beads. 11 cycles Kapa PCR.