title: GSM5273341: MCF7 cells – control 0 hr – rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: After treatment, cells were harvested, maintained on wet ice, pelleted and flash frozen in liquid nitrogen.  Samples were kept on dry ice until Qiagen Rneasy kit (Qiagen, cat no. 74104) was utilized to extract total RNA for RNA-seq.  Samples were stored at -20°C until library preparation and sequencing. RNA libraries were prepared using standard Illumina protocols, including oligo-dT purification