title: GSM5469097: Basidia_replicate_3; Melampsora larici-populina; RNA-Seq
library_construction_protocol: Total RNA from BSD samples were isolated with the QIAgen RNeasy Plant Minikit from several rounds of RNA isolation using low inputs of material (10 to 20mg). Total RNA from PYC and AEC samples were isolated from 100mg infected larch needles using a protocol adapted to RNA isolation from conifers (Chang et al 1993 Plant Mol Biol doi: 10.1007/BF02670468). cDNA synthesis and RNA-Seq library construction were performed by Beckman Coulter Genomics using the Illumina TruSEQ RNA seq kit.