title: Illumina MiSeq paired end sequencing; Enrichment and characterization of human otic sensory progenitors derived from induced pluripotent stem cells
design: Enrichment and characterization of human otic sensory progenitors derived from induced pluripotent stem cells
library_construction_protocol: The undifferentiated hiPS cell line (ChiPSC-4) was derived from fibroblasts of healthy human donors and reprogrammed by using polycistronic retrovirus, based on the transduction of Oct3/4, Sox2, Klf4, and c-Myc transcription factors. The day 6 and day 13 hiPSCs-OSPCs were produced by stepwise differentiation of hiPSCs in adherent monolayer cultures. Total RNA were extracted from cells using the PureLink® RNA Mini Kit (Life Technologies) according to manufacturer's instructions. The RNAseq libraries are constructed according to the TrueSeq Stranded (Illumina) protocol, using 500ng of total RNA