title: GSM5981731: Input [0A5K_Pooled_Input]; Homo sapiens; ChIP-Seq
library_construction_protocol: B-EBV cells (10x106) from P3 and a healthy control C1 were fixed with freshly prepared 1% formaldehyde (#F-8775, Sigma) for 15 min and quenched with 0.125M glycine (Sigma #G-7403) for 5 min, washed twice with 1x PBS containing 0.5% Igepal CA-630 (Sigma #I-8896), the second time in the presence of 1M Phenylmethanesulfonyl fluoride. Cell pellets were snap frozen and subjected to ChIP-seq. Chromatin extraction, IP using IRF4 antibody (Cell Signalling, #4948), library preparation, next generation sequencing and a Model-based Analysis of ChIP-Seq (41) was performed by Active Motif (Carlsbad, CA)