title: GSM5915509: HSC on AAVS1 with AAV Mouse 54; Homo sapiens; OTHER
library_construction_protocol: gDNA was isolated with QIAamp DNA Micro Kit (QIAGEN) according to the manufacturer's instructions. PCR amplicons for individual samples were generated by nested PCR using primers listed in the related manuscript and starting from >50-100 ng of purified gDNA. The first PCR step was performed with GoTaq G2 DNA Polymerase (Promega) according to manufacturer instruction using the following amplification protocol: 95°C x 5'min, (95°