title: GSM5273589: episomal_MPRA_DNA_rep2; Escherichia coli DH5[alpha]; OTHER
library_construction_protocol: The plasmid library was extracted from electroporated E. coli using the the ZymoPure II Plasmid Maxiprep Kit. RNA samples was first converted to cDNA by reverse transcription with the SuperScript IV First Strand Synthesis Systems (Invitrogen) using oligo dT primers. Barcodes were amplified from the respective plasmid/genomic DNA/cDNA samples using custom primers. Illumina sequencing adapters were then further added on 2 rounds of PCR with custom primers.