title: GSM5231618: AF16 rep2 control; Caenorhabditis briggsae; RNA-Seq
library_construction_protocol: Collected worms were homogenized in 1 ml Tri-reagent for 30 min at room temperature. 0.1 mL of BCP was added to the sample and mixed well. The sample was then spun at 12,000 g 15 min at 4°C and the aqueous phase was transferred to a new tube. 0.5 ml Isopropanol was added to the sample, incubated at room temperature for 10 min and spun at 12,000 g 10 min. The RNA pellet was washed twice with 75% EtOH and dissolved in water. The RNA sample was then was then purified to remove DNA using RNeasy Mini Kit (Qiagen) The protocol for library preparation was using KAPA RNA hyper prep kit (Roche)