title: Illumina MiSeq paired end sequencing of SAMD00138666
library_construction_protocol: "DNA was subjected agarose gel and OD ratio tests to confirm the purity and concentration prior to Bioruptor NGS (Diagenode Inc., Denville, USA) or Covaris (Covaris, Inc., Woburn, MA USA) fragmentation. Fragmented gDNAs were tested for size distribution and concentration using a Bioanalyzer 2100 or Tapestation 2200 (Agilent Technologies, Santa Clara, CA USA) and Nanodrop 2000 (Thermo Scientific, Waltham, MA USA). The fragmented gDNA was performed for ~400-700bp size selection. Illumina libraries were made from qualified size selected gDNA using SPRIworks HT-High Throughput Sample Preparation for Illumina NGS Platforms (Beckman Coulter, Inc. Indianapolis IN). The resulting libraries were tested for size distribution and concentration by an Agilent Bioanalyzer 2100. The samples were then sequenced on an Illumina Miseq (Illumina, San Diego, CA USA) with V2 PE250 reagents kit which generated paired-end reads of 250 nucleotides (nt)."