title: Illumina HiSeq 2000 paired end sequencing; Chromatin structure changes upon oxidative stress or UV irradiation in the yeast Saccharomyces cerevisiae
design: Chromatin structure changes upon oxidative stress or UV irradiation in the yeast Saccharomyces cerevisiae
library_construction_protocol: Yeast from glycerol stocks were streaked out onto agar plates with complete YPD media and incubated at 30°C. One colony was picked and grown over night in a 10ml of YPD at 30°C while shaking at 220 RPM. The culture was then diluted in 100ml of YPD and grown 30°C with shaking until mid-log phase.Cells were synchronized in G1 with alpha-factor and then incubated 30 min until reaching S phase. Oxidative stress was performed by incubating the cells 10min with 10mM H2O2 at 30ºC and UV irradiation was done 5min under 254nm UV. Hi-C protocol from Belton et al, 2012, with minor modifications Sample prep Hi-C protocol