title: Whole genome sequencing with Illumina for Escherichia coli HUM3355
design: To extract genomic DNA, cells were lysed by boiling with detergent, followed by ethanol precipitation of DNA as previously described in [DOI: 10.3389/fmicb.2018.01894]. Short-read library preparation was conducted using the NEBNext Ultra DNA library kit for Illumina. Libraries were sequenced on the MiSeq platform using Reagent Kit v3 (600-cycle, Illumina Inc.), producing 150 bp paired-end reads