title: GSM5111239: WT DMSO, 3xTg DMSO, WT SCDi, 3xTg SCDi; Mus musculus; RNA-Seq
library_construction_protocol: Mice received a lethal dose of ketamine (Bimeda-MTC)/xylazine (Bayer Healthcare) and were then perfused transcardially with phosphate-buffered saline (PBS) followed by rapid dissected of the ipsilateral hippocampi from each mouse into ice cold HBSS. Hippocampi were dissociated into single cells and each sample was pooled to obtain 4 mice per group, then the cells were labeled with CD45 mouse immune multiplexing kit (BD Biosciences, USA), counted and 10 000 cells per sample were multiplexed, ready for single-cell capture. The viability of cells in all samples was 80-95%. Single-cell capture was performed on the BD Rhapsody Single-Cell Analysis System (BD, USA), the DNA from each cell attached to a barcoded bead was subsequently retrieved and libraries were prepared using the whole transcriptome analysis (WTA) amplification kit (BD Biosciences) according to the manufacturer's recommendations. Single cell RNA libraries were generated using the BD Rhapsody 3' WTA kit with sample multiplexing using the BD Mouse Immune Single-Cell Multiplexing Kit Single cell RNAseq