title: GSM5824331: Human tumor 1; Homo sapiens; RNA-Seq
library_construction_protocol: Tissue was washed in an RBC lysis buffer, passed through a 70 μm filter, and washed in PBS. Cells were dissociated with cold DNAse and proteases and titrated every 5-10 minutes to increase dissociation. Tumor 1: . Library preparation for scRNA-seq was performed according to previous protocols (Banerjee et al., 2020), and cells were sequenced using BGI MGI-seq.   Tumor 2: Tumor was immediately placed in cold RPMI on ice for dissociation. Library preparation for scRNA-seq was performed as described previously (Banerjee et al., 2020). Cells were prepared for sequencing using TruDrop (Southard-Smith et al., 2020) and sequenced on Nova-seq.