title: GSM5273201: minus 2 hr rep 6; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol Reagent (Thermo-Fisher Scientific) using the manufacturer's protocol with some exceptions. 100 mL of sand (Sigma-Aldrich) was included to aid homogenization. Sand was first prepared by washing two times in 1 M HCl, washed eight times in RNAse-free water (to a neutral pH), and baked to dry. Libraries were prepared for sequencing using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, E7530) with 100 ng of starting RNA per library and 14 cycles of PCR. Libraries were sequenced using Illumina HiSeq 4000.