title: GSM5825388: AC16 cell line-C2_IgG; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. After the DNA sample passes the test, it is randomly interrupted by covaris ultrasonic crusher, and then repaired at the end, added with a tail and added with a tail，The whole library was prepared by sequencing, purification and PCR amplification. After the library is built, qubit2.0 is used first perform preliminary quantification, dilute the library to 1.5ng/ul, and then use Agilent 2100 to detect the insert size of the library. After the size meets the expectation, the effective concentration of the library is accurately quantified by Q-PCR (the effective concentration of the library is > 2nm), to ensure the quality of the library.