title: Illumina HiSeq 2500 sequencing; RNA-Seq of 60 Neuroendocrine Neoplasm (NEN) samples with different grade and primary site
design: RNA-Seq of 60 Neuroendocrine Neoplasm (NEN) samples with different grade and primary site
library_construction_protocol: Out of 60 RNAs, 19 derived from FFPE sections (Formalin fixed paraffin embedded), 21 from frozen sections and 20 were previously extracted RNAs from MTC patients. RNA isolations were performed from both FFPE and frozen sections using FFPE DNA Purification Kit (Cat. 47400, Norgen Biotek Corp) and RNA/DNA Purification Kit (Cat. 48700, Norgen Biotek Corp.) respectively, according to the manufacturers protocol. Libraries preparation for transcriptome analysis was performed employing the TruSeq RNA Exome kit (Cat. 20020189, Illumina) for FFPE samples and TruSeq Stranded Total RNA kit (Cat. 20020598, Illumina) for frozen samples, starting from 200ng and 400ng of RNA as input materials, respectively, according to manufacturers' guidelines.