title: GSM6283636: AGH118_11; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Sonicated chromatin extracts were immunoprecipitated with anti-histone H3, anti-Gcn4 (home-made), anti-TBP (from Joseph Reese's lab), anti-myc (Sigma, Cat# 11667203001), anti-Rpb1 (Biolegend, Cat#664906) and anti-Rpb3 (NeoClone, Cat# W0012). Immunoprecipitated chromatin were treated with proteinase K and DNA were extracted with chloroform and precipitated with iso-propanol. Libraries were prepared using DNA Library Prep Kit for llumina (Cat# E7370) from New England Biolabs.