title: GSM4830979: scDesLO D17; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were prepared as described by the 10x Genomics Single Cell 3′ v2 Reagent Kit user guide. Day 17 DesLO were acquired in single cell suspension by incubation with trypsin for 10 minutes at 37°C , followed by gentle pipetting using a serological pipette to dislodge and dissociate aggregates. Two washes in PBS -/- + 0.04% BSA were performed with the cells re-suspended at a final concentration of 1000 cells/μL in PBS -/- + 0.04% BSA. A live cell count was performed with a hemocytometer using Trypan Blue to identify dead cells. Following counting, cells and 10x Genomics reagents were loaded into the single cell cassette, with a target of 6000 single cells for analysis, accounting for predicted cell loss and doublets as laid out in the user guide for the Chromium Single-Cell 3' Reagent Kit (10x Genomics).  After generation of GEMs, the cDNA library was prepared by ASU Genomics Core staff following the appropriate steps determined by the 10x Genomics user guide.