title: ITS-Seq  of Rhizophora mucronata root-associated mycobiomes in Gazi Bay, Kenya
design: Total genomic DNA was extracted directly from 0.25 g of soil using Power Soil DNA isolation kit. Amplification of the ITS regions was performed using a two-step dual-indexed PCR approach specifically designed for Illumina instruments. The PCR reaction was purified using the Axygen PCR cleanup kit. Barcoded Amplicons were pooled in equimolar concentration for sequencing on the illumina Miseq.