title: GSM5273374: ATAC-seq_MCF7 cells – TBOOH 8 hrs – rep 2; Homo sapiens; ATAC-seq
library_construction_protocol: After treatment, cells were harvested, maintained on wet ice, and counted.  50000 cells / ml was used: first washed in ice cold PBS and resuspended in 50 μL ice cold lysis buffer.  Cells were centrifuged at 500 x G for 10 min at 4°C to generate crude nuclei pellets. The crude nuclei pellet was resuspended in 50 μL Nextera Tn5 reaction buffer and transposase (Nextera cat no 15027865-6).  They were then incubated at 37°C for 30 min at 250 RPM, purified with Qiagen MinElute PCR Purification Kit, eluted in 50 μL buffer EB and stored at -20°C until library preparation and sequencing. DNA libraries were prepared using standard Illumina protocols for Nextera-tagged DNA