title: GSM6122148: 16hpi, ptetEuo, rep2; Chlamydia trachomatis; RNA-Seq
library_construction_protocol: C. trachomatis infected HeLa were disolved in TRIzol (300 ul/ well in 24 well plate). After centrifugation, the aqueous layer containing the total RNA was loaded on the Qiagen column and supplier instructions were followed hereafter. RNA was DNase treated on the column and eluted in MQ water. After RNA extraction, the samples were analyzed by Qubit to check for any degradation of the RNA. The yield of the RNA samples was measured on a Qubit. RNASeq library prepared using Zymo-seq RiboFree Total RNA Library Kit by Zymo Research, with 10ng of total RNA