title: GSM5273608: TRIP_mapping_DNA_pool4_rep1; Homo sapiens; OTHER
library_construction_protocol: DNA and RNA was harvested simultaneously using the TRIzol reagent (Life Technologies) following the manufacturer's protocol. Genomic DNA was digested overnight with XbaI, SpeI, NheI and AvrII (New England Biolabs). The digested DNA was then ligated overnight with T4 DNA ligase (New England Biolabs). Custom primers were used to perform inverse PCR on the ligated molecules to amplify the barcodes and genomic region where the transposon is integrated. Illumina sequencing adapters were then added on with 2 rounds of PCR with custom primers.