title: GSM5915027: C35P_24_Exp3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Homogenized plant material of five replicate samples per condition was pooled to reach 100 mg separately for each of the three independent experiments resulting in 54 samples. Total RNA was isolated using a “single step” Trizol based method (Chomczynski and Sacchi, 2006) with modifications as previously described (Sprenger et al., 2016). Four µg of RNA were treated with DNAse (RapidOut DNA-removal Kit, Thermo Fisher Scientific, Schwerte, Germany) and the absence of genomic DNA in the samples was confirmed using qPCR using intron-specific primers (Zuther et al., 2012). The RNA quality and integrity was verified by the Agilent 2100 Bionalyzer (Agilent Technologies, Santa Clara, CA). NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs)