title: GSM4830395: Ctrl_3rd (EIF5A2 control); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIZOL (Ambion). The RNA was further purified with two phenol-chloroform treatments and then treated with RQ1 DNase (Promega, Madison, WI, USA) to remove DNA. The quality and quantity of the purified RNA were redetermined by measuring the absorbance at 260 nm/280 nm (A260/A280) using Smartspec Plus (BioRad, USA). The integrity of RNA was further verified by 1.5% agarose gel electrophoresis. RNA libraries were prepared for sequencing using standard Illumina protocols