title: GSM5701519: Ptch1 positive subpopulation rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNA extraction kit (Qiagen, Valencia, CA, USA) according to manufacturer's instructions. Truseq Stranded Total RNA Libraries were generated from 500ng of total RNAs using Truseq Stranded Total RNA kit (Illumina). Libraries were then quantified with Qubit and pooled. 4nM of this pool were loaded on a High Output Flowcell and sequenced on a NextSeq500 platform (Illumina) with 2x75bp paired-end chemistry.