title: Bolinopsis infundibulum pooled larvae PacBio Iso-Seq FLNC
design: Total RNA was isolated from approximately 10 mg of 18-hour-post-fertilization B. infundibulum embryos using a Trizol RNA isolation protocol (Rio et al. 2010). The RNA was converted to a PacBio Iso-Seq library and sequenced on one Sequel II SMRT cell at the Brigham Young University DNA Sequencing center. This yielded 2,691,284 CCS Iso-Seq reads, with a mean read length of 3.0 kb and a read N50 of 3.1 kb. Primers and barcodes were removed with Pacific Biosciences lima v2.2.0 (github.com/PacificBiosciences/barcoding), and 2,623,057 full-length non-chimeric (FLNC) reads were generated with Pacific Biosciences isoseq3 v3.4.0 (github.com/PacificBiosciences/IsoSeq).