title: GSM5111105: d8-1C RNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq : RNA was harvested from ES cells using Trizol reagent. MedSeq : Genomic DNA was digested with LpnPI. RNA-Seq samples were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit. The resulting DNA libraries were sequenced according to the Illumina TruSeq Rapid  v2 protocol on an Illumina HiSeq2500 sequencer, generating single-end reads of 50bp. MedSeq : LpnPI-digested genomic DNA was processed using the ThruPlex DNA–seq 96D kit (Rubicon Genomics cat#R400407). Stem‐loop adapters were blunt end ligated to repaired input DNA and amplified (4 +10 cycles) to include dual indexed barcodes to yield an indexed Illumina NGS library. Multiplexed samples were sequenced on Illumina HiSeq2500 systems for single read of 50 base pairs.