title: GSM5008415: OS6_3 Bent stage Seed coat RNA-seq replicate 3; Brassica oleracea; RNA-Seq
library_construction_protocol: Embryo isolations were performed using a dissecting microscope, fine forceps and needles in a 4.8% Sucrose solution that contained 0.1% RNALater solution.For seed coat isolation, the embryos were manually removed, and the remaining seed coat was kept. Total RNA was extracted from each stage embryo sample following the protocol of RNAqueous-micro kit. RNA-seq libraries were prepared using the NEBNext Single Cell/Low Input RNA library Prep Kit for Illumina.