title: GSM5519453: AS3MT_WT_D4_2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lifted with Accutase and split into two 1.5ml tubes, one for DNA and other for RNA. Tubes were spun at max speed for 1m to pellet cells before removing media. Pellets for RNA were resuspended in TRIZol reagent before extraction using TRIzol and Direct-zol miniprep kit (Invitrogen, Carlsbad, CA, USA). cDNA libraries were generated using the TruSeq DNA HT Library Preparation Kit (Illumina Inc., San Diego, CA, USA)