title: GSM6284405: N1_Mouse3; Homo sapiens; RNA-Seq
library_construction_protocol: 8 days post-transfer, 300 CD4+ EGFRt+ CAR-T cells were FACS-sorted from peripheral blood of tumor-bearing mice into SMART-Seq HT lysis buffer (Takara). cDNA was generated using 13 amplification cycles and purified using AMPure XP beads (Agencourt). Libraries were prepared using the Nextera XT kit (Illumina) and sequenced on a HiSeq (Illumina) to read depths >10x106 per sample.