title: GSM5655232: Silenz_II_Exp_R1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using mini RNase kit reagent (Qiagen, Tübingen, Germany). Poly-A enriched library were generated with the TruSeq RNA-Seq Library Preparation Kit v2 (#RS-122-2001, Illumina) according to the manufacturer's instructions. Library quality control was performed using the Agilent 2100 Bioanalyzer (Agilent Technologies). Indexed libraries were sequenced at CRS4 Next Generation Sequencing facility with the HiSeq 3000 instrument to generate ~40M 50bp single-end reads per sample.