title: GSM5272903: Acetabular cartilage, DDH; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted with Isogen (Nippon gene, Japan) and RNeasy spin column kit (Qiagen, USA). 1 µg of RNA samples with RNA integrity number (RIN) greater than 7 were used for library preparation. RNA-Seq libraries were prepared using SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian (Takara Bio Inc.) according to the manufacturer's instructions and subsequently validated for an average size of about 410-430 bp using a 2100 Bioanalyzer and the Agilent DNA1000 kit for the construction of sequencing libraries.