title: GSM4829873: PPs2 (Bisulfite-Seq); Rattus norvegicus; Bisulfite-Seq
library_construction_protocol: Colonic epithelia (>95%) were obtained by scraping at 4 0C . Genome DNA was extracted with gDNA Extraction Kit (Omega Bio-Tek Research，USA) and sheared to fragments of 150-200 bp (Covaris E-series instrument, Australia). DNA libraries were prepared with Agilent SureSelectXT Methyl-seq Library Pre kit and then hybridized with hybridization reagents, blocking agents and the SureSelect capture library.