title: GSM5702268: MM088; Homo sapiens; RNA-Seq
library_construction_protocol: Bone marrow aspirate samples were collected at baseline prior to the induction treatment of VMP or VTD. BM aspirates were subjected to a Ficolle-Paque PLUS gradient, and isolated mononuclear cells were cryopreserved in freezing medium before the experiments. The single-cell library preparation relied on a commercially available droplet method, the 10x Genomic Chromium System (10x Genomic Inc., San Francisco, CA), with 10x Genomics Single Cell 3' v3 Reagent kit according to the manufacturer's protocol. In brief, dissociated cells were counted by hemocytometer (ThermoFisher) and 16,000 cells per sample were added to each channel. The cells were then partitioned into gel beads in emulsion (GEMs) in the Chromium instrument, where cell lysis and barcoded reverse transcription of RNA occurred. cDNA was synthesized and amplified for 14 cycles. cDNA clean-up was performed using a SPRIselect Reagent Kit (Beckman Coulter, Brea, CA). 50 ng of the amplified cDNA were used for each sample to construct indexed sequencing libraries.