title: NextSeq 500 paired end sequencing; Epigenetic control of Lgr5 + intestinal cancer stem cells by the histone methyltransferase Mll1
design: Epigenetic control of Lgr5 + intestinal cancer stem cells by the histone methyltransferase Mll1
library_construction_protocol: Ls174T cells were trypsinized for 3min 37Â°C and collected by centrifugation, cells were resuspended in RNA lysis buffer (NucleoSpin RNA isolation kit (Macherey-Nagel). three shMLL1 Ls174T cell clones were cultured in DMEM 10% FBS 1% PenStrep with/without 300ng/ml doxycycline for 3 days Total RNA was isolated with the NucleoSpin RNA isolation kit (Macherey-Nagel). Samples were subjected to library preparation using the TruSeq stranded mRNA library preparation kit.