title: GSM5111565: Human fibroblast; Homo sapiens; RNA-Seq
library_construction_protocol: GEMs were generated by combining barcoded gel beads, a RT master mix containing cells, and partitioning oil onto Chromium Chip G. Following full length cDNA synthesis and barcoding from poly-adenylated mRNA, GEM were broken and pooled before cDNA amplification by PCR using 11 cycles. After enzymatic fragmentation and size selection, sequencing library were constructed by adding Illumina (Paris, France) P5 and P7 primers as well as sample index via end repair, A tailing, adaptor ligation and PCR with 10 cycles. Library quantification and quality control was performed using Bioanalyzer 2100 (Agilent Technologies, Santa Clara CA). Single cell 3 prime mRNA seq library were generated according to 10X Genomics User Guide Chromium NEXT GEM Single Cell 3' Reagent Kits v3.1 (P/N CG000204 Rev D).