title: GSM5653512: D105T48 culture 3h; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lyzed and mRNA extracted using Qiagen RNEasy Mini kit including on-column DNAse digestion. Libraries were prepared according to Illumina's instructions accompanying the TruSeq Stranded mRNA Library Prep Kit (Illumina). 500 ng of RNA was used for each sample. Sequencing was performed in 13 sequencing unit of NovaSeq 6000 (Illumina) (100-nt-length reads, paired end) with an average depth of 40 millions of clusters per sample.