title: Metatranscriptomic sequencing of Mus musculus cecal microbiome
design: Total RNA was first depleted of bacterial ribosomal and host reads using the MICROBExpress kit (Invitrogen) and the NEBNext rRNA depletion kit for mouse/rat/human (New England BioLabs). Enriched RNA was then prepared into sequencing libraries with the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturers instructions