title: GSM5315689: 134_GS1513_C_DHF_pos_400; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed in 8 μL of lysis buffer composed by 0.2% Triton X-100, 2 U/ul of recombinant RNase inhibitor (Clontech/Takara), 5 mM dNTP mix (Life Technologies) in 0.2 ml PCR tube (Maxymum recovery, Axygen). RNA extraction, reverse transcription, cDNA amplification, fragmentation, additional PCR amplification, final library purification, and quality control were performed as described (methodology section). Sequencing was performed according to a paired-end strategy using the HiSeq2500 sequencer (Illumina). Samples that failed quality control standards were eliminated from further analysis21. Smart-seq2