title: GSM5272764: ∆A1S_1874 Mutant2; Acinetobacter baumannii; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNAeasy Mini kit (QIAGEN) following the manufacturer instructions. RNA integrity was assessed using the Agilent Technologies 2100 Bioanalyzer (or 2200 TapeStation) (Agilent Technologies, CA, USA).Sequencing libraries were generated using TruSeq Stranded Total RNA (NEB Microbe) library kit and NovaSeq 6000 S4 Reagent Kit following manufacturer's recommendations. mRNA was purified from total RNA using ribo-zero rRNA removal kit. Fragmentation was carried out using divalent cations under elevated temperature.Reverse transcribe  onto cDNA. Nat Rev Genet. 2011 Sep 7;12(10):671-82 RNA libraries were prepared for sequencing using standard Illumina protocols