title: GSM6119014: nSyb, ZT14, R2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: We used the Gal4-UAS system to label with GFP either clock neurons (CLK856-Gal4) or all neurons (nsyb-Gal4) and then purified them using fluorescence activated cell sorting (FACS). Neurons of both types were collected at two circadian time points - ZT02 and ZT14 in duplicates. cDNA was generated using SMARTseq2 protocol, which was then used to make sequencing libraries using the Illumina Nextera XT Library Prep Kit