title: GSM5588682: Enhancer_hybrid_select_BioReplicate3_TechnicalReplicate9.; Homo sapiens; OTHER
library_construction_protocol: We extracted RNA using 3 RNAeasy columns per 50-million cell replicate and isolated polyA+ mRNA using the Qiagen Oligotex mRNA kit. We performed reverse transcription using SuperScript IV, then performed a junction PCR using intron-spanning primers, then finally a sequence-ready PCR.