title: Illumina MiSeq paired end sequencing
library_construction_protocol: The second (of a two-step)PCR amplicons were purified using Agencourt AMPure XP Beads (Beckman Coulter Inc., CA, USA) and quantified using a Qubit 3.0 Fluorometer and by agarose gel electrophoresis. MID-tagged amplicons were then pooled in equimolar amounts for library construction. Libraries were sent to the Norwegian Sequencing Centre (Oslo, Norway) for paired-end (2 x 300bp) HTS on a MiSeq platform (Illumina, CA, USA) using the MiSeq Reagent Kit v3 (Illumina) kit.