title: GSM5586961: siCtrl_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using a standard TRIZOL/Phenol/Chloroform protocol. RNAs resolubilised in 20µL of RNase Free water were then passed through an RNA purification column (from the Macherey Nagel Ref:740955.250 kit) and subjected to rDNAse digestion from the purification kit. Libraries were prepared using the NEB Ultra II protocol (stranded polyA+ RNA-seq) and sequenced with an Illumina NextSeq 500 as 2x75 bp paired-ends (PE)