title: Salpingoeca rosetta ATCC PRA-366 Hi-C MluCI
design: Toward scaffolding the already-published genome assemblies to chromosome-scale, we prepared Hi-C libraries from Salpingoeca rosetta (ATCC PRA-366), Capsaspora owczarzaki (ATCC 30864), and Creolimax fragrantissima (ATCC PRA-284). The American Type Culture Collection (ATCC) samples that we used are the same accessions that were sequenced for the published genome assemblies for those three species (Fairclough et al. 2013; Suga et al. 2013; de Mendoza et al. 2015). We ordered one cell culture for each of the three ATCC accessions listed above. We briefly thawed each sample, aspirated 0.125 mL of cell culture stock per library, and prepared Hi-C libraries using a previously published Hi-C library preparation protocol (Adams et al. 2020). For each species we prepared one library with the enzyme DpnII and one library with MluCI. These libraries were sequenced on a NovaSeq 6000 2x150 cycle run at MedGenome, Inc. in Foster City, California. 97.76 ng into the FS reaction. Directly onto Strep beads right after adapter ligation.