title: NextSeq 500 paired end sequencing; Multigene panel test in 44 Homo sapiens patients with either  digestive system neuroendocrine Neoplasm (NEN) or medullary thryoid gland carcinoma samples
design: Multigene panel test in 44 Homo sapiens patients with either  digestive system neuroendocrine Neoplasm (NEN) or medullary thryoid gland carcinoma samples
library_construction_protocol: Out of 44 tumor biopsies, 18 were FFPE sections (Formalin fixed paraffin embedded) while 26 were frozen sections. DNA isolations were performed from both FFPE and frozen sections using FFPE DNA Purification Kit (Cat. 47400, Norgen Biotek Corp) and RNA/DNA Purification Kit (Cat. 48700, Norgen Biotek Corp.) respectively, according to the manufacturers protocol. Libraries for mutational profiling were prepared, starting from 40ng of DNA as input material, with TruSightTM Oncology500 kit (Cat. 20028213, Illumina) according to manufacturer's protocol.