title: WT2 RNA
design: RNA sequencing (RNAseq) of total extracts from hippocampal cell culture was performed by Single End (SE) sequencing using NextSeq2000 (Illumina) sequencer with V2 chemistry. RiboGeneTM-Mammalian (Clontech) was used to deplete ribosomal RNA from the samples. SMARTer Universal Low Input RNA Kit for Sequencing (Clontech) was used for pre-amplification before library preparation. Library preparation was performed using the Low Input Library Prep Kit (Clontech) following the manufacturer's instruction.