title: GSM6283898: D10 M1; Homo sapiens; ncRNA-Seq
library_construction_protocol: Extracellular vesicles (EVs) were isolated from the conditioned media of M1 and M2 macrophages using size exclusion chromatography. Total RNA was extracted from EVs using a Total RNA Purification Kit (Cat No. P4-0058 – 17200, Norgen), following the supplementary protocol for exosomal RNA. RNA samples were prepared for small RNA sequencing using Qiagen's QIAseq small RNA Library Prep kit.