title: GSM5824340: 102xPR.IND2; Homo sapiens; RNA-Seq
library_construction_protocol: Cell were collected in ice-cold lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 100 μg/ml cycloheximide, 1% v/v Triton X-100 and 25 U/ml Turbo DNase I), triturated ten times through a 26-gauge needle and centrifuged for 10 min at 20,000 g at 4 °C. Total RNA was isolated by Zymo Research Direct-zol RNA MiniPrep Kit. RNA libraries were prepared using TruSeq mRNA Library Prep with polyA selection. Libraries were sequenced on the Illumina NovaSeq 6000 (100bp Single Read, 20M reads) following the manufacturer's protocols.