title: GSM5111130: SOX9_siRNA_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: PANC-1 cells were transfected in 24-well plates with either 25 nM of control siRNA (Dharmacon catalog # D-001210-03-05) or 25 nM of SOX9 siRNA (Dharmacon catalog # M-021507-00-0005) using Lipofectamine 3000 (Thermo). After 48 hours, transfected PANC-1 cells were pooled and harvested, two replicates from each siRNA condition, and total RNA was isolated using a Qiagen RNeasy kit. RNA-seq libraries were created using the Illumina TruSeq Stranded Total RNA Sample Prep Kit. RNA-seq libraries were pooled and sequenced on the Illumina HiSeq 2000 to a minimum depth of 60 million 2x100 bp reads per library. Illumina TruSeq Stranded Total RNA Sample Prep Kit