title: 16S rRNA gene V3-V4 hypervariable regions: fishery pond sediments
design: Total genomic DNA was isolated from sediment samples using the ZymoBIOMICS  DNA Miniprep Kit (Zymo Research, USA) according to the manufacturer's recommendations. The concentration of extracted DNA was evaluated using a biophotometer (Eppendorf, Germany). The DNA samples were sent to Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) to amplify V3-V4 16S rRNA regions and sequence on Illumina paired-end platform to generate 250 base pairs (bp) length paired-end raw reads. Note: the uploaded sequences are with adapter and barcode sequences removed.