title: GSM5272987: FB-16-B1: GBM Tumor - Control - Rep3; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were isolated and dissociated with Tumor dissociation Kit (Miltenyi Biotec). After debris removal procedure (Debris Removal Solution, Miltenyi Biotec) donor-derived CD45+ cells were sorted. Single-cell RNA-Seq libraries were generated using a microfluidics-based approach on Chromium Controller (10X Genomics) using the Chromium Single Cell 3' Reagent Kit v2.0 according to the manufacturers' instructions. Briefly, sorted single cells were suspended in 0.4% BSA-PBS, at a concentration ranging from 1000 to 2000 cells/µL. 8,700 cells were added to each channel in order to achieve a recovery rate of 5,000 cells per sample. Cells were partitioned in Gel Beads in Emulsion (GEMs) and lysed, followed by RNA barcoding, reverse transcription and PCR amplification (13 cycles).