title: Illumina NovaSeq 6000 paired end sequencing; Selection of RNA-seq library preparation protocol for a comprehensive transcriptome analysis in T acute lymphoblastic leukemia (T-ALL)
design: Selection of RNA-seq library preparation protocol for a comprehensive transcriptome analysis in T acute lymphoblastic leukemia (T-ALL)
library_construction_protocol: T-cells (T-ALL and normal T lymphocytes) were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson). Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research).  Libraries were generated with Illumina TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat. Modified fragmentation conditions were used (90'C for 2 minutes; referred to as P2).