title: GSM5736791: scRNA_E13.5_WT_1; Mus musculus; RNA-Seq
library_construction_protocol: Embryonic hearts of E13.5 and E17.5 were minced and enzymatically dissociated using 2 mg/mL type 2 collagenase, 1 mg/mL dispase, and 20 U/mL DNase I, with 5 cycles of digestion for a total 40 min at 37 °C. After using a 40-μm cell strainer to remove the debris and multiplets, 5,000 cells were prepared to a concentration of 1000 cells/µL and loaded into the Chromium Controller. single-cell cDNA libraries were generated using the Chromium 3'  v3 chemistry kit (10x Genomics) according to the manufacturer's instruction. Libraries were sequenced on a NovaSeq 6000 System (Illumina) using a NovaSeq S4 Reagent Kit (200 cycles, 20027466, Illumina).