title: GSM5272769: SP-WT; Acinetobacter baumannii; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNAeasy Mini kit (QIAGEN) following the manufacturer instructions. Sequencing libraries were generated using Ribo-Zero rRNA Removal Kit (Bacteria), TruSeq RNA Sample Prep Kit v2 following manufacturer's recommendations. Ribo-Zero User Guide, TruSeq RNA sample prep v2 Guide, Part # 15026495 Rev. F was used for making library protocol. mRNA was purified from total RNA using ribo-zero rRNA removal kit. Fragmentation was carried out using divalent cations under elevated temperature.Reverse transcribe  onto cDNA. Nat Rev Genet. 2011 Sep 7;12(10):671-82 RNA libraries were prepared for sequencing using standard Illumina protocols