title: GSM5425745: WT saline 3 control; Mus musculus; RNA-Seq
library_construction_protocol: Ventral lobes were thawed and the RNA was stabilized in RNAlater (Thermo Fisher Scientific) for 1 hour. Total RNA was isolated from ventral lobes using the Qiagen RNeasy Micro kit with on-column DNAse digestion (Germantown, MD). RNA quantity was measured with NanoDrop and assayed on an Agilent 2100 Bioanalyzer. The cDNA library was prepared by the TruSeq Stranded Total RNA (Gold) Library Prep with after polyA enrichment and rRNA reduction. Libraries were quantified using Qubit DNA HS kit and assayed on Agilent Tapestation DNA 1000 system.