title: Bulked Segregant Analysis of Apost-1 suppressor of bps1-2
design: lIlumina TruSeq Nano DNA Sample Prep (550 bp mean insert size): DNA samples (200 ng) were sheared using a Covaris S2 Focused-ultrasonicator and libraries were prepared using the Illumina TruSeq DNA PCR-Free Sample Preparation Kit (cat# FC-121-4001 and FC-121-4002) with an average insert size of 550 bp.  PCR-amplified libraries were qualified on an Agilent Technologies 2200 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  The molarity of individual libraries were normalized to 10 nM and equal volumes were pooled in preparation for Illumina sequence analysis.