title: GSM5915187: ATAC-Seq OCI-AML3 NPM1c degron2 cell line treated with 500nM dTag-13 rep1; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 cells were harvested and permeabilized in 50 μL of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). The transposition reaction was carried out at 37°C for 30 min with agitation in 50 μL volume containing 25 μL of 2× TD buffer and 2.5 μL of Nextera Tn5 transposase. DNA was purified with Qiagen MinElute PCR purification kit. Library amplification was done with KAPA HiFi HotStart ReadyMix PCR kit, and the resulting libraries were purified with Qiagen MinElute PCR Purification Kit. Libraries were sequenced in the Illumina HiSeq 4000 platform to generate paired-end reads of 2 × 150 bp. Library construction protocol is described in Himadewi. et al. [PMID: 34585664]