title: Shotgun sequencing of Physaria filiformis sample AR_1-16
design: DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol (Doyle & Doyle, 1987). DNA was fragmented using a Qsonica q800R, and library preparation was conducted using NEBNext Ultra DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina. The indexed samples were sequenced using 2150 bp reads on an Illumina Miseq.