title: Illumina HiSeq 1500 sequencing; Transcriptional changes in IMR32 cells upon ISX treatment
design: Transcriptional changes in IMR32 cells upon ISX treatment
library_construction_protocol: cells from ATCC. For sequencing: Triplicates were pooled in equal amounts into one sample. IMR32 grown in DMEM (high Glucose) +PenStrep +10%FBS Day0.5: Change to 0.5%FBS +DMSO or ISX (20ÂµM) Day2.5 Harvest RNA-prep with Machery-Nagel spin columns and on-column DNAse digest Illumina TruSeq total RNA v2