title: GSM5111127: SOX9_ChIP_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Approximately 2.0 X 10^8 cells were crosslinked in 11% formaldehyde and stopped with 2.5 M glycine before being washed in 1X PBS, lysed, and sonicated for 35 minutes in a Bioruptor at 4C to achieve a fragment size of approximately 200 bp. An input fraction was set aside and the rest of the lysate was then incubated with 10 ug anti-SOX9 (AB5535, Millipore) overnight at 4C. Antibody-bound chromatin was then purified using Protein G Dynabeads (Thermo) and crosslinking was reversed overnight at 65C. ChIP-seq libraries were created using the Illumina TruSeq DNA Sample Prep Kit and quantified using Quant-iT PicoGreen dsDNA assay (Invitrogen). ChIP-seq libraries were pooled and sequenced on the Illumina HiSeq to a minimum depth of 59 million, 1x50bp reads per library. Illumina TruSeq DNA Sample Prep Kit