title: S_1_c
design: The V4 hypervariable region of the 16S rRNA gene was amplified using dual indexed Illumina compatible primers 515f/806r as described by Kozich, JJ (2013)[1]. PCR products were batch normalized using Invitrogen SequalPrep DNA Normalization plates and the products recovered from the plates pooled. The pool was cleaned up and concentrated using AmpureXP magnetic beads; it was QCd and quantified using a combination of Qubit dsDNA HS, Agilent 4200 TapeStation HS DNA1000 and Kapa Illumina Library Quantification qPCR assays. The pool was loaded onto an Illumina MiSeq v2 standard flow cell and sequencing was performed in a 2x250bp paired end format using a MiSeq v2 500 cycle reagent cartridge. Custom Sequencing and index primers were added to appropriate wells of the reagent cartridge as described in [1]. Base calling was done by Illumina Real Time Analysis (RTA) v1.18.54 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.