title: Metabarcoding of Bacteria: rapeseed soil
design: 2-step amplicon PCR on 16S V4-V5 region with primers 16S_515f (GTGCCAGCMGCCGCGGTAA) and 16S_806r (GGACTACVSGGGTATCTAAT) with incorporation of barcodes on 2nd PCR, followed by purification and quantification to build equimolar pools diluted with PhiX phage mix to 12 pM