title: GSM5273494: GE1_oviducts of Yun7Ge; Bombyx mori; RNA-Seq
library_construction_protocol: Total RNA was prepared using TRIzol (Invitrogen) from the mixture of three whole oviduct of Yun7 and Yun7Ge before mating, and two replicates were set for each sample. RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using Qubit® RNA Assay Kit in Qubit®2.0 Flurometer (Life Technologies, CA, USA). RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations. RNA libraries were prepared for sequencing using standard illumina protocols