title: GSM4830210: Negative_female_4; Oreochromis niloticus; RNA-Seq
library_construction_protocol: pituitaries were hravested and digested by trypsin into a single cell suspension, The cell suspensions were sorted in a FACSAriaIII sorter using a 488nm and 561nm lasers to excite the GFP and RFP labelled cells respectively, Total RNA was prepered using Trizol® Reagent (Invitrogen) , RNA libraries were prepared using the SMARTer® Stranded Total RNA-Seq Kit v2- Pico Input Mammalian (Takara Bio, USA)