title: GSM5273248: Warming F10L3 WGBS-seq; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted and purified from each plant using a MiniBEST Plant Genomic DNA Extraction Kit (TaKaRa, Dalian, China). Genomic DNA was fragmented to 200–300 bp with a Covaris sonicator, followed by end repair and adenylation. The purified DNA fragments were bisulfite converted using the EZ DNA Methylation-GoldTM Kit (Zymo Research). Library concentration was quantified by Qubit® 2.0 Flurometer (Life Technologies, CA, USA) and quantitative PCR, and the insert size was assayed on Agilent Bioanalyzer 2100 system.