title: 18S rRNA amplicon sequencing of freshwater metagenome from a dimictic lake
design: Between 75-100 ml of water filtered onto 0.22 _m filters. DNA was extracted with DNEasy Powersoil DNA Isolation kit. DNA was quantified with a Nanodrop. 18S rRNA sequencing for eukaryotic algae was performed on an Illumina MiSeq using 2x250 bp chemistry. The V9 region 18S rRNA was amplified using primers 1391F (5-GTACACACCGCCCGTC-3) and EukBr (5-TGATCCTTCTGCAGGTTCACCTAC-3).