title: GSM4830977: atacDesLO-2; Homo sapiens; ATAC-seq
library_construction_protocol: Cultures were acquired in single cell suspension by incubation with trypsin for 10 minutes at 37°C, followed by gentle pipetting using a serological pipette to dislodge and dissociate aggregates.  Cells were frozen in mFresR cryopreservation medium. ATAC-Seq library preparation and sequencing reactions were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA). Live cell samples were thawed, washed, and treated with DNAse I (Life Tech, Cat. #EN0521) to remove genomic DNA contamination. Live cell samples were quantified and assessed for viability using a Countess Automated Cell Counter (ThermoFisher Scientific, Waltham, MA, USA). After cell lysis and cytosol removal, nuclei were treated with Tn5 enzyme (Illumina, Cat. #20034197) for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (QIAGEN, Cat. #28004) to produce tagmented DNA samples. Tagmented DNA was barcoded with Nextera Index Kit v2 (Illumina, Cat. #FC-131-2001) and amplified via PCR prior to a SPRI Bead cleanup to yield purified DNA libraries.