title: GSM5008319: human_NPC_Day8_Rep1_RNA; Homo sapiens; RNA-Seq
library_construction_protocol: Nextera DNA Library Prep Kit (20018704, Illiumina) Between 19 and 38 ng of RNA were converted to cDNA using the Smart-Seq 2 protocol (Picelli, et al., Nat. Protoc., 2014). Libraries were constructed with the Nextera DNA Library Prep Kit (FC-121-1030, Illumina) for control-3, control-4, and FSHD2-2 libraries, and the Nextera DNA Flex Library Prep Kit (20018704, Illumina) for control-1, control-2 and FSHD2-1 libraries. Libraries were sequenced on the Illumina NextSeq500 platform using paired-end 43 bp mode. Cells were lifted using Accutase. Total RNA was extracted by using the RNeasy kit (QIAGEN). DNA was extracted by using omni ATAC protocol previously described