title: Bolinopsis infundibulum MBA F3 Individual PacBio CLR SMRT Cell 1 of 2
design: High molecular weight (HMW) DNA was isolated from B. infundibulum individual Binf1 by lysing tissue in CTAB buffer (Dawson, Raskoff, and Jacobs 1998), then purifying with a with a chloroform, phenol:chloroform, chloroform, ethanol precipitation protocol (Sambrook and Russell 2006). The HMW DNA was sent to Brigham Young University DNA Sequencing Center, where one PacBio CLR library was constructed and sequenced on two Sequel II 15-hour SMRT cells.