title: GSM4830517: OCI-AML3_single-cell_12; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA for cell bulk controls was extracted from Kasumi-1 and OCI-AML3 cell bulk samples using the AllPrep DNA/RNA Kit (Qiagen) NGS libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 20 ng of partially fragmented epi-gSCAR amplicons (Kasumi-1 single cell 1-7), 50ng ng of fragmented epi-gSCAR amplicons (Kasumi-1 single cell 8-27 and OCI-AML3 single cell 1-20) or 50ng ng of fragmented Kasumi-1 and OCI-AML3 bulk gDNA according to the manufacturer's recommendations with 50% reduction of reaction volumes.