title: GSM5980039: WT_A1; Mus musculus; RNA-Seq
library_construction_protocol: To sort CD45-TER119-CD31-CD140a+CD51+MSCs, bones (femora and tibiae) were crushed, digested with 2 mg/mL collagenase (Wako) in Dulbecco's Modified Eagle Medium for 60 min at 37℃ and sorted using the FACS AriaⅡ. The libraries were constructed using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 according to the manufacturer's protocol. Reverse transcription and library preparation were performed on a C1000 Touch Thermal cycler with 96-Deep Well Reaction Module (Bio-Rad). Amplified cDNA and final libraries were evaluated on an Agilent BioAnalyzer using a High Sensitivity DNA Kit (Agilent Technologies). The libraries were sequenced on a NextSeq Illumina 500 platform using NextSeq 500/550 High Output v2.5 Kit (150 cycles). scRNA-seq