title: Illumina HiSeq 2500 paired end sequencing; RNA-seq for gene expression changes upon silencing MEF2D-fusion in a MEF2D-HNRNPUL1 fusion-expressing Kasumi-7 cell line
design: RNA-seq for gene expression changes upon silencing MEF2D-fusion in a MEF2D-HNRNPUL1 fusion-expressing Kasumi-7 cell line
library_construction_protocol: A MEF2D-HNRNPUL1-fusion-expressing cell line Kasumi-7 was genome-edited so that the fusion is tagged with HAtag-2A-GFP. The resulting cells were infected with shRNA lentivirus for GFP to knock-down the fusion. shRNA for luciferase was used as a control. Cells were collected after the infection. Total RNA was extracted using RNA easy micro plus kit (Qiagen) The cDNA library was made using the NEBNext Ultra Directional RNA Library PrepKit (New England BioLabs)