title: GSM5588540: BMDMs, WT 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from treated BMDM was isolated using Trizol reagent and RNeasy kits (QIAGEN). Libraries for mRNA sequencing were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara). Full-length cDNAs were processed using a Nextera XT DNA Library Preparation Kit (Illumina). Quality control was performed using a High Sensitivity DNA ScreenTape Assay with a 4200 TapeStation System (Agilent). Libraries were then multiplexed in an equimolar pool and sequenced using a NextSeq 550 Platform (Illumina). mRNA sequecing