title: GSM5702367: cuttag_control_ siRNA_BRD7Ab; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA after immunoprecipitation was extracted by NovoNGS CUT&Tag High-Sensitivity Kit (Novoprotein, N259-YH01, Shanghai, China) following manufacturer's protocol. 100,000 cells were used for CUT&Tag assay. After binding with Concanavalin A beads, cells were incubated with primary antibody targeting BRD7 (abeam, ab56036). Samples were then incubated with Goat anti-Rabbit antibody, CUT&Tag Assembled pA-Tn5 Transposomes. After tagmentation, released DNA was extracted by TIANSeq Size Selection DNA Beads (TIANGEN, NG316, China). DNA library was perpared by illumina primers provided with NovoNGS CUT&Tag High-Sensitivity Kit.