title: GSM5315575: Placenta, Patient 899; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from all samples was prepared using RiboZol reagent followed by Rneasy Mini Kit purification, following manufacturer protocol. RNA purity was confirmed by NanoDrop Spectrophotometry and integrity confirmed by Agilent BioAnalyzer. Only RNA with a RIN > 8.0 was used for sequencing. Libraries were constructed using the 10X Genomics chromium single cell 3' reagent v2 chemistry kit for all placental tissue samples and using the chromium single cell 3' reagent v3 chemistry kit for all trophoblast organoid samples at a read depth of 50,000 reads/cell for placental villus samples and a read depth of 150,000 reads/cell for trophoblast organoid samples. Single-cell RNA-seq