title: Donggang rhizosphere soil wild
design: To generate bacterial libraries, the first round of PCR was performed to amplify the V3-V4 region of the 16S rRNA gene using universal primers 341F (5- CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3)which contains Nextera consensus and adaptor sequences at the forward (5- TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-target sequence-3) and reverse (5- GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-target sequence-3) (Fadrosh, et al., 2014). The PCR products were cleaned-up and amplified with primers containing Illumina dual indices and sequencing adapters using the forward index i5 (5-AATGATACGGCGACCACCGAGATCTACAC-55555555-TCGTCGGCAGCGTC-3) and reverse i7 (5-CAAGCAGAAGACGGCATACGAGAT-7777777-GTCTCGTGGGCTCGG-3). Purified amplicon libraries were pooled and sequenced at ChunLab Inc. (South Korea) with an Illumina MiSeq system using the MiSeq Reagent Kit v2 (Illumina Inc., USA).