title: GSM6283964: MLN iILC2, unbound fraction of meRIP, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested from the IL-25-induced iILC2 in MLN using miRNeasy Micro Kit (Qiagen). Then m6A immunoprecipitation (meRIP) was performed to obtain the input, unbound and pellet fractions. 30 ng of RNA of input, unbound or pellet fractions was used for the construction of sequencing libraries. cDNA libraries for RNA-seq were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina® following manufacturer's protocols.