title: GSM4829576: shCtrl-1; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq and m6A MeRIP-seq, total RNA from cells in culture was extracted using Trizol reagent, and then treated with DNase I to remove DNA contamination. m6A MeRIP were next performed and the m6A-enriched fragmented RNA was purified using RNeasy Mini Kit as IP. For RIP-seq, HCT116 cells were harvested after 24 h transfection of Flag-tagged YTHDF1 plasmid. The protein was extracted with RIP lysis buffer. YTHDF1-RNA complex was captured by FLAG antibody-immobilized-protein A/G magnetic beads and enriched RNAs were extracted by TRIzol as IP. For RNA-seq, RNA libraries were constructed with TruSeq Stranded mRNA Library Prep kit. For RIP-seq and m6A MeRIP-seq, RNA libraries were prepared using SMARTer Stranded Total RNA-Seq Kit version 2-Pico Input Mammalian.