title: GMNL-855  sequencing by NGS
design: For the GMNL-855 and BCRC-16100 strains, 1 g DNA per sample was used as the input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following the manufacturers recommendations, and index codes were added to attribute sequences to each sample. In brief, the DNA sample was fragmented through sonication to the size of 350 bp, and DNA fragments were then end-polished, A-tailed, and ligated using the full-length adaptor for Illumina sequencing with further PCR amplification. The PCR products were purified (AMPure XP); the libraries were then analyzed for size distribution by using an Agilent 2100 Bioanalyzer and quantified using real-time PCR. The clustering of the index-coded samples was performed on a cBot Cluster Generation System according to the manufacturers instructions. After cluster generation, the library preparations were sequenced on an Illumina NovaSeq 6000 platform, and paired-end reads were generated.