title: GSM5653840: WGBS_Parental_wt; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA was extracted from 4M cell pelets using DNeasy Blood and Tissue (Qiagen). 1000 ng of genomic DNA was spiked with 0.1% (w/w) unmethylated λ DNA (Roche Diagnostics) previously fragmented to 300–400 bp peak sizes using the Covaris focused-ultrasonicator E210. Fragment size was controlled on a Bioanalyzer High Sensitivity DNA Chip (Agilent) NxSeq AmpFREE Low DNA Library Kit (Lucigen)and bisulfite converted using the EZ-DNA Methylation Gold Kit (Zymo Research)