GSM5114442: C2C12_shPerk_D3; Mus musculus; miRNA-Seq
Total RNA was extracted from C2C12 cells or P19 cells with TRIzol reagent according to the manufacturer's instruction. The total RNA or purified SRNA fragments of the samples were extracted and connected to the 3-terminal and 5-terminal joints first and then reverse-transcribed into cDNA. PCR amplification was carried out and then the gel was cut to recover the target fragment library. Computer sequencing was performed on the library that passed the quality inspection.