RNA-Seq of Musa acuminata AAA Group:Tai-Chiao No. 7 plantlet Fusarium oxysporum f. sp. cubense tropical race 4 inoculation for 3 days replicate 3
Tai-Chiao No. 7 plantlets were grown at 28 degrees Celsius under 60 micromol m-2 s-1 irradiance (16 h light and 8 h dark). 8~10 week old plantles were inoculated with Fusarium oxysporum f. sp. cubense tropcial race 4 for 3 days. Total RNA was extracted from roots with PureLink Plant RNA Reagent (Thermo Fisher Scientific), was used in library construction for RNA-seq profiling. The RNA-seq libraries were prepared using the Illumina NEBNext Ultra RNA Library Prep Kit. (http://www.illumina.com/).