GSM5399104: TRIM28 ChIP-Seq, TNF treated for 4hr; Homo sapiens; ChIP-Seq
Lysates were prepared from ~5 millon single cell dissociated of organoid cultures or monolayer cultures. Sonicated nuclei with Bioruptor were further proceeded to ChIP assay with apporiate antibodies. Libraries were prepared using the NEXTflex ChIP-seq kit (Cat# NOVA-5143-02;  PerkinElmer) according to the manufacturer's instructions.