GSM5655267: P18 PG1337 1ug/ml 48h; Homo sapiens; RNA-Seq
Organoids were lyzed with Cell Lysis buffer and RNA was purified using PureLink® RNA Mini Kit RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 12 cycles of amplification.