Single cell transcriptome of human gamma delta T cells in the course of CMV reactivation, timepoints C and E
Gamma delta T cells were sorted from frozen PBMCs sampled at indicated timepoints and then mixed at a ratio of 1:1. For discrimination of cells from different timepoints, TotalSeq-C hashtag antibodies from Biolegend were used to stain the cells together with fluorochrome labelled antibodies. The generation of the single cell libraries was then performed according to the procedure of the 10xGenomics workflow.