GSM5702375: 2102EP PRT4165; Homo sapiens; RNA-Seq
RNA was isolated (including DNase I digestion) using the RNAeasy Mini Kit (Qiagen, Hilden, Germany) according to the manual RNA samples used for transcriptome analyses were quantified (Qubit RNA HS Assay, Thermo Fisher Scientific, Meerbusch, Germany) and quality was measured by capillary electrophoresis using the Fragment Analyzer and the â€˜Total RNA Standard Sensitivity Assayâ€™ (Agilent Technologies Inc., Santa Clara, USA). The library preparation was performed according to the manufacturerâ€™s protocol (â€˜VAHTS Stranded mRNA-Seq Library Prep Kitâ€™). 300Â ng totalÂ RNA were used for mRNA capturing, fragmentation, synthesis of cDNA, adapter ligation, and library amplification. Bead purified libraries were normalized. RNA sequencing performed on the HiSeq 3000 system (Illumina Inc., San Diego, USA) with a read setup of 1x150Â basepairs (bp).