scRNA-seq of B16 tumor: CD45+ sorted cells
From each animal, 20,000 CD45+ live cells were sorted using a 6-laser BD FACSAria II cytometer equipped with a 70-mm nozzle, and samples from the same condition were pooled. Next, 5,000 cells per genotype were targeted for droplet formation using a Chromium Single Cell System (10x Genomics). Samples were processed according to the manufacturers protocol (10x Genomics, Chromium Next GEM Single Cell 5 Library, PN-1000167).