GSM5657396: ChIPseq IP 1056∆ Rep A; Cereibacter sphaeroides 2.4.1; ChIP-Seq
Cells lysed and DNA sheared with sonication, DNA-protein fragments trimmed with micrococal nuclease and Rnase treated, Antibody coimmunoprecipitation, sepharose bead pull down, 250mM LiCl wash, 600mM NaCl wash, 300mM NaCl wash, TE buffer wash, protein-DNA complexes eluted from beads, crosslinking revered by heating, DNA purified with QIAquick PCR purification Kit ChIP-seq library preparation and sequencing was performed at GeneWiz. Libraries for sequencing were created using the Illumina TruSeq ChIP library preparation kit (Illumina) following the standard protocol. ChIP-seq libraries were sequenced on an Illumina HiSeq 3000 2x150bp reads using the standard protocol.