GSM6209238: Mature antheridia pinC#29 (biol rep 3); Physcomitrium patens; RNA-Seq
Samples were collected using thin forcettes and stored directly in TRIzol ® (Fisher Scientific GmbH, Schwerte, Germany) on wet ice. Tissues were homogenized with a small pistill and mixed with chloroform. The aqueous phase was further processed according to Direct-zol RNA Microprep Kit (Zymp Research Europe GmbH, Freiburg, Germany). Extracted RNA was treated with Ribolock RNase Inhibitor (Fisher Scientific GmbH, Schwerte, Germany). cDNA library was created at Genomics Unit, Instituto Gulbenkian de Ciencia, Portugal according to (Picelli et al 2014:Full-length RNA-seq from single cells using Smart-seq2. Nature Protocols 9: 171–181.)