GSM5009533: liver_slice_L30C8; Homo sapiens; Mus musculus; RNA-Seq
Cell lysis and amplification. In-situ reverse transcription on spatially barcoded microwell array. Pooling cells off array and FACS sorting for secondary indexing. Cell lysis, reverse transcription template switching and barcoded cDNA amplification. cDNA tagmentation amplification with primers containing short-read sequencing adapters (Illumina). Combinatorial indexing for single cell barcoding. Initial barcoding step provides 2-dimensional spatial index.