Illumina HiSeq 2000 paired end sequencing; Single cell RNA sequencing from extra-thymic Aire-expressing cells (Cd31-gp38-Cd45+EpCam+MHC+GFP+) sorted from mixed/pooled lymph nodes from 2 C57 BL/6 mice  (Homunculus project).
Single cell RNA sequencing from extra-thymic Aire-expressing cells (Cd31-gp38-Cd45+EpCam+MHC+GFP+) sorted from mixed/pooled lymph nodes from 2 C57 BL/6 mice  (Homunculus project).
"Liberase digestion of LN (same concentration as for thymus); depletion of TCRb, CD3, F4/80, CD8, B220, Dx5 and CD19 +ve cells by AutoMACS" "Cells were sorted directly into 96 well plates containing lysis buffer, followed by reverse transcription and cDNA preparation as per Smart-seq protocol" "Nextera XT. Spike-in sequences and their concentrations can be found in the ""Additional files"" attached to this ArrayExpress experiment."