GSM6209445: WT-III-3; Danio rerio; RNA-Seq
Oocytes from adult's ovary were collected using physical method and RNA was extracted using Trizol ( Invitrogen™). For each sample, 200ng of total RNA was used for synthesis and amplification of cDNA, and 1ng of amplified cDNA was used for construction of sequencing library using VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina.