GSM5962720: Astrocytes, APP-3; Mus musculus; miRNA-Seq
Trizol total RNA isolation Kit was used to extract total RNA following the manufacturer's instructions. The concentration and integrity of RNA were determined by Nanodrop  and Agilent 2100 Bioanalyzer. RNAs were first ligated with a 3′ adapter followed by size fraction and ligation to 5′ adapter. Then, the small RNA libraries were constructed and sequenced using Illumina Novaseq6000 by Gene Denovo Biotechnology Co. (Guangzhou, China).