Plastic bottle site 1 1 year
PCR amplifications and Miseq Illumina sequencing of the regions V3-V4 of the 16S rRNA and the region V4-V5 of the 18S rRNA of each of the three replicates of each sample (192 sequenced samples) were carried out by the Genomics Service of the Parque Cientifico de Madrid (Madrid, Spain). The used primers are shown in Table S2.  DNA libraries and amplicon sequencing were performed as previously described in Martinez-Campos, et al., (2018)