GSM5398932: E9_ATAC_rep2; Mus musculus; ATAC-seq
limbs were dissociated using 100ug/mL Liberase (Roche 05401119001) in PBS at 37 degrees for 6 minutes. Libraries were prepared with the following number of amplification cycles, for each given experiment: CUT&Tag/Run- 14 cycles, ChIP- 15 cycles, ATAC- 11 cycles. Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads. ChIP & CUT&RUN library- NEBNext Ultra II DNA Library Prep Kit; ATAC & CUT&Tag- NEBNext HF 2x PCR Master Mix; RNA-seq- NEBNext Ultra II RNA Library Prep Kit