GSM5398850: MII_A3; Mus musculus; RNA-Seq
After washed three times with 0.1% BSA/PBS, each oocyte was then transferred into 0.2 mL PCR tubes containing 2 μl lysis buffer. Transcriptome libraries were prepared following the Smart-seq2 protocol. Sequencing libraries were constructed by using KAPA HyperPlus Kit (Kapa Biosystems) according to the manufacturer's instructions.