GSM5702705: u87_3009a_rep1; Homo sapiens; RNA-Seq
RNA was extracted using Trizol following manufacturer's protocol 250 or 500 ng total RNA was poly-A selected by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and then converted to libraries by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #7760) according to the manufacturer's protocol. The resulting libraries were quality checked for concentration and size distribution before pooling for paired-end sequencing on Illumina HiSeq by Novogene.