GSM6210238: W308R ABCA3 mutant iAEC2s containing the ABCA3:GFP fusion reporter, rep1; Homo sapiens; RNA-Seq
FACS sorting for single, live, ABCA3:GFP+ cells RNA extraction was done using QIAzol (Qiagen) RNeasy Kit Sequencing libraries were prepared from total RNA extracted from each indicated sample using Illumina TruSeq RNA Sample Preparation Kit v2. mRNA was isolated using magnetic bead-based poly(A) selection, fragmented, and randomly fragmented for reverse transcription, followed by synthesis of cDNA fragments. cDNA fragments were then end-paired, and ligated to Illumina Paired-End sequencing adapters. The products were end-paired and PCR-amplified to create the final cDNA library.