all_protozoa_d2
The PCR reaction was purified using AmpureXP beads, and then a second PCR was performed using the Fluidigm Access Array primers for Illumina to add the adaptor and index sequences. For this reaction 2 ul of the first PCR were amplified in a 10 ul reaction for 10 cycles. The PCR reaction was purified using AmpureXP beads and the concentrations were measured by Qubit. The samples were pooled, run on a DNA D1000 screentape (Agilent) to check for correct size and for the absence of primer-dimers product. The pool was then sequenced on the Illumina Miseq, using the Miseq V2-500 cycles sequencing kit