GSM5398922: E10.5_Gli3null-ant_RNAseq_rep3; Mus musculus; RNA-Seq
limbs were disscociated in TRIzol (Invitrogen 15596026), incubated at RT for 5 minutes and then stored at -80C prior to RNA isolation Libraries were prepared with the following number of amplification cycles, for each given experiment: CUT&Tag/Run- 14 cycles, ChIP- 15 cycles, ATAC- 11 cycles. Excess primers were removed from PCR reactions using AmpureXP beads (A63880) at a ratio of 0.8x beads. ChIP & CUT&RUN library- NEBNext Ultra II DNA Library Prep Kit; ATAC & CUT&Tag- NEBNext HF 2x PCR Master Mix; RNA-seq- NEBNext Ultra II RNA Library Prep Kit