GSM4891272: MAC_PROseq_LPS 1h_rep2; Homo sapiens; OTHER
After washing the cells with DPBS three times, total RNA was extracted by directly adding TRIzol  to the plate with the adherent cells on it. 5 million cells were used for the construction of PRO-seq libraries. For 3′-seq and TED-seq, 10 ug of total RNAs were used. RNA libraries were prepared to be compatible with Illumina small RNA sequencing. We prepared PRO-seq libraries as described in Kwak et. al. (2013) Science.