GSM5589277: HCC38-siCT-N2; Homo sapiens; RNA-Seq
Cells were grown in P150 plates and treated with AR antagonists. At harvest time, cells were washed twice with cold PBS1x (Invitrogen), scraped and collected in eppendorfs. Cells were spun down, washed once more with cold PBS1x, removing the supernatant, followed by snap freezing in liquid nitrogen. Frozen cell pellets (1 x 10^6 cells) were used for RNA extraction using the commercial RNeasy Kit (Qiagen). The quality of RNA was assessed using a bioanalyzer  Eukaryote Total RNA Nano chip. Samples that scored above RIN:9 were used to generate the mRNA library. Samples were submitted for pair-end sequencing at Macrogen Oceania PL. The next-generation sequencing data were processed on the Illumina System.