GSM4890494: Biopsy 1, PCM1+ nuclei; Homo sapiens; RNA-Seq
After tissue homogenization, nuclei were isolated by sucrose gradient centrifugation and stained with a PCM1 antibody. PCM1+ and PCM1- nuclei were sorted using FACS. Nuclear suspensions were loaded into the 10x Genomics Chromium Controller for library preparation. Libraries were prepared for approximately 4000 nuclei per sample. Sequencing of libraries was conducted on a NovaSeq 6000 instrument targeting >50,000 reads per nuclei.