GSM4891280: MAC_TEDseq_LPS 1h_rep2; Homo sapiens; OTHER
After washing the cells with DPBS three times, total RNA was extracted by directly adding TRIzol  to the plate with the adherent cells on it. 5 million cells were used for the construction of PRO-seq libraries. For 3′-seq and TED-seq, 10 ug of total RNAs were used. RNA libraries were prepared to be compatible with Illumina small RNA sequencing. We prepared TED-seq libraries as described in Woo et. al. (2018) Cell Reports.