GSM4891109: BMSC Donor 3, 7-days TGF-B1 exposure, analyzed on Day 7_rep1; Homo sapiens; RNA-Seq
Samples were crushed in RLT buffer (Qiagen) containing β-mercaptoethanol (Sigma) and RNA was isolated using the RNeasy Mini Kit (Qiagen) with on-column DNase I (Qiagen) digestion, as per manufacturer's instructions. Illumina's TruSeq stranded RNA sample preparation protocol was used for processing samples. This protocol includes the following steps:  cDNA Synthesis: Purification of mRNA (via oligo(dT) beads), Fragmentation of mRNA with divalent cations and heat 1st strand cDNA synthesis (randomly primed), 2nd strand cDNA synthesis; and   cDNA library preparation: DNA fragment end repair (blunt ending of DNA fragments), 3' Adenylation of DNA fragments, Sequencing adapter ligation (utilizing T-A pairing of adapter and DNA fragments), Amplification of library via PCR.