GSM5399434: ER-Hoxa9-Lys-GFP (-) E2 72 hours Rep 2; Mus musculus; ATAC-seq
Nuclei isolated from 100,000 cells using 100 ul hypotonic lysis buffer to generate two independent transposition reactions. Nuclei were split in half and treated with 2.5 ul Tn5 Transposase (Illumina) for 30 mins at 37 degrees celsius. DNA from transposed nuclei was isolated and PCR-amplified using barcoded Nexteral primers (Illumina). Library QC was carried out using a high sensitivity DNA bioanalyzer assay and quibit measurement.