GSM5962449: 2: gastrocnemius, knockout; Mus musculus; RNA-Seq
Muscle were harvested and immediately snap frozen in liquid nitrogen. Total RNA was isolated using RNA STAT-60 as per manufacterer's instructions. RNA  was DNase I treated as per manufacturer's instructions (RNase-Free DNase Set, Qiagen) then cleaned up and eluted with RNAse and DNAse free molecular grade water (RNeasy MinElute Cleanup Kit, Qiagen), followed by quantification. KAPA RiboErase