V9-18S rDNA-seq of Eukaryotic: seawater-12m eDNA
Successful amplicons were puried using paramagnetic KAPA pure beads (beads-to-sample volumetric ratio in 1.6:1). A Qubit fluorometer with Qubit dsDNA HS Assay reagent (Invitrogen, California, US) was used to quantify all libraries. The prepared samples were combined in equal concentrations and then pooled with a 20% denatured and diluted PhiX Illumina control library. The final pooled library was sequenced on an Illumina MiSeq with the MiSeq v2 500-cycle kit (Illumina, San Diego, CA, United States).