GSM5917207: Seq03_Tfh2_1e4_5; Mus musculus; RNA-Seq
Cells were stained for flow cytometry and and sorted directly into Trizol LS. RNA was isolated using Chloroform-Extraction followed by a RNA-purification via spin-columns. Libraries were generated according to manufacturer´s guidelines with the 3' mRNA-Seq Library Prep Kit FWD with Unique Dual Indices (Lexogen) using 50 ng total RNA as input.