GSM5589068: RRBS Dnmt3a KO rep5; Mus musculus; Bisulfite-Seq
RRBS libraries were made from 300 ng of purified DNA using Zymo-Seq RRBS Kit according to manufacturer instructions. ATAC sequencing libraries were made according to manufacturer instructions (Active Motif). ChIP-sequencing libraries were made according to manufacturer instructions (SimpleChIP, Cell Signaling) but cells were also sheared to 100-300 bp using Covaris sonicator. Libraries were constructed per protocol for Nextseq 550 or Novaseq SP.