GSM5114197: K562_PCGF1KO_R1; Homo sapiens; RNA-Seq
Total RNA was extracted according to the manufacturer's recommendations using RNeasy Plus Mini Kit (Qiagen, Cat#74134) or High Pure RNA Isolation Kit (Roche, Cat#11828665001). Libraries were prepared using Roche Kapa mRNA HyperPrep strand specific sample preparation kits from 200ng of purified total RNA according to the manufacturer's protocol on a Beckman Coulter Biomek i7. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer's protocols. Uniquely dual indexed libraries were pooled in equimolar ratios and sequenced on an Illumina NextSeq 550 with single-end 75bp reads or a NovaSeq 6000 with 50bp read pairs.