GSM5277009: Fig4-de_ATACSeq_DddIA-ND6_rep2; Homo sapiens; ATAC-seq
10,000 viable cells were pelleted, lysed and the genomic DNA was tagmented with 2.5μl self-assembled Tn5 transposase. The tagmented DNA was purified using the DNA Clean and Concentrator-5 Kit (Vistech) and eluted in 20μl ultrapure water. All 20μl eluate was amplified by 12 cycles in a total volume of 50μl and the final library was purified and size-selected using Agencourt AMPure XP beads (Beckman Coulter).