GSM5009219: ES WT Hi-C; Mus musculus; Hi-C
10^6 cells were cross-linked for 10 min with 1% final concentration fresh formaldehyde and quenched by 0.2M glycine for 5 min. The cross-linked cells were subsequently lysed in lysis buffer. Extracted nuclei were re-suspended with 150 μl 0.1% SDS and incubated at 65°C for 10 min, then SDS were quenched by 120 μl water and 30 μl 10% Triton X-100, and incubated at 37 °C for 15 min. Purified DNA was sheared to a length of ~400 bp. Point ligation junctions were pulled down by Dynabeads MyOne Streptavidin C1(Thermofisher) according to manufacturers` instructions. The Hi-C library for Illumina sequencing was prepared by NEBNext Ultra II DNA library Prep Kit for Illumina (NEB) according to manufacturers` instructions. The final library was sequenced on the Illumina HiSeq X Ten platform (San Diego, CA, United States) with 150PEmode. Two replicates were generated for one group material.