RNA-seq of Locusta migratoria manilensisadult
Crude virus purification was performed by a discontinuous sucrose gradient.Total viral RNA was extracted using TRIzol LS reagent (Invitrogen, Carlsbad, CA) according to the manufacturers instructions.  Residual Ribosomal RNA (rRNA) was depleted using Ribo-Zero kits (Epicentre, Madison, WI) before library construction. Libraries were constructed using a TruSeq total RNA library preparation kit (Illumina) and paired-end (250-300bp) sequencing was performed on the Hiseq-PE150 platform (Illumina, Sandiego, CA).