GSM4891304: ChIP-seq LAPC4 AR EtOH rep2; Homo sapiens; ChIP-Seq
Cells were crosslinked for 10 min with 1% formaldehyde and quenched with 125 mM glycine for 5 min. Cells were then washed with PBS, pelleted, flash frozen and stored at -80°C. Lysates were clarified from sonicated nuclei and AR- or KLF4-DNA complexes were isolated with antibody. Libraries were prepared according to NEB's instructions accompanying the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®. After adapter ligation, DNA was PCR amplified for 12 cycles. The purified DNA was captured on an S4 Flowcell (PE100) for cluster generation. Libraries were sequenced on the Illumina NovaSeq 6000 following the manufacturer's protocols at the next generation sequencing platform of Centre de Recherche du CHU de Québec (CRCHUQ).