GSM4903794: 7498 MethylC-seq; Spirodela polyrhiza; Bisulfite-Seq
Bisulfite-Seq: 1ug of DNA was used to prepare libraries as previously described (Urich et al. 2015). ChIP Seq:1 g of fresh duckweed plantlets were crosslinked in 1% formaldehyde for 10 min. Nuclei were then isolated and sonicated for 15 min, twice. Histone-DNA complexes were pulled down with anti-H3K9me2 (Cell Signaling Technology antibody #9753s). DNA was isolated and used to prepare ChIP-seq libraries with the TruSeq ChIP Library Preparation Kit (Illumina, IP-202-1012), including a gDNA input control. Sequencing was performed on an Illumina NextSeq500 in Georgia Genomics and Bioinformatics Core (GGBC) in the University of Georgia