23_3_R4_SynC_Ramp
Tri-Reagent as per manufacturers instructions including a DNAse treatment step. Dual-indexed, strand-specific RNASeq libraries were prepared from submitted total RNA, using RiboZero Plus rRNA depletion and Ultra II Directional RNA library preparation kits. The raw reads were pre-processed including the removal of low-quality bases and sequencing adapters (CGR, Liverpool).