GSM5917159: RRBS_Patient Derived Xenografts UPN01 at diagnosis, rep 3; Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted using the Wizard SV Genomic DNA purification system, following manufacturer's instruction and including a step of protein lysis using Proteinase K. Genomic DNA was digested using methylation-insensitive restriction enzymes to generate short fragments enriched for CpG dinucleotides at the extremities. After end-repair, A-tailing and ligation to methylated adapters, the CpG-rich DNA fragments were subjected to size selection, bisulfite conversion, and PCR amplification.