GSM6171485: Veh1_A375; Homo sapiens; ATAC-seq
Harvested cells from each condition were lysed in 50 µl ATAC lysis buffer (Tris 10 mM pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40) and incubated with Illumina Tn5 transposase at 37⁰C for 30 mins. Samples were purified using Agencourt AMPure XP beads following which barcoding and library generation were performed using the NEBNext Q5 Hot Start HiFi PCR Master Mix with PCR amplification for 12 cycles. The size selected libraries were run on a HiSeq2500 to get an average of 40-50 million paired end reads per sample.