GSM6123685: SH-SY5Y WT PROLI 3 (smallRNA-seq); Homo sapiens; ncRNA-Seq
Total RNA was isolated using the Quick-RNA MiniPrep Kit (Zymo Research, R1055) according to the manufacturer's protocol. 500 ng of total RNA were used to prepare small RNA sequencing libraries using Small RNA-Seq Library Prep Kit (Lexogen, Vienna, Austria) following the manufacturer's protocol. Library quality was analyzed with High Sensitivity DNA Kit on Agilent Bioanalyzer 2100, and quantification was done using Qubit dsDNA HS (High Sensitivity) Assay kit (InvitrogenTM) with Qubit fluorometer. Quantified libraries were sent to the Lexogen sequencing facility (Vienna, Austria). Single-end read sequencing was carried out with 75 bp sequencing length on the Illumina NextSeq500 platform.