GSM5655369: Md-OB_3; Mus musculus; RNA-Seq
Mandible and tibia bones from P9 Col1a1*2,3-GFP mice were dissected. Three independent dissections, each pooling bone tissues from approximately fifty P9 mice, were carried out. Immediately after dissection and collagenase I digestion, GFP-positive cells were FACS-sorted. RNA was extracted using single cell RNA purification kit (Norgen). RNA quantification and integrity were performed with Bioanalyzer RNA (Agilent). Based on RNA quantification and RIN number, 3 experiments with RNA quantity ranging from 2 to 8ng were selected for RNAseq analysis. RNAseq was performed by Oxford Gene Technology (OGT). Generation of cDNA was performed using SMART-Seq v4 Ultra Low Input RNA Kit (Takara) and processed with Illumina TruSeq RNA Sample Preparation kit (Illumina). RNA seq data were generated on Illumina HiSeq 2000 sequencing instrument according to the manufacturer's instructions