GSM6210116: dual; Homo sapiens; RNA-Seq
On day 7 post-transduction, cells were prepared for single-cell RNA-sequencing as detailed in the 10x Genomics Single Cell Protocols Cell Preparation Guide (10x Genomics, CG00053 Rev C) and separated into droplet emulsions using the Chromium Controller (10x Genomics) with Chromium Single-Cell 3′ Gel Beads v3.1 (10x Genomics, PN-1000121 and PN-1000120) across 12 lanes/gemgroups with the goal of recovering ~15,000 cells per GEM group before filtering. Sequencing libraries were prepared following the 10x Genomics Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) with Feature Barcoding technology for CRISPR Screening (CG000205; Rev C). 10x Genomics 3' v3.1