GSM5655672: RNAi3 [ATAC-seq]; Trypanosoma brucei brucei; ATAC-seq
Cells were washed, permeabilized with 40 uM digitonin for 5 min at RT, and incubated with Tn5 transposase for 30 min at 37ºC. DNA was purified using Qiagen MinElute Kit Amplification was carried out with 2× KAPA HiFi mix and 1.25 uM of Nextera primers