GSM4891978: Kr-h1 ChIP - worker - rep 2; Harpegnathos saltator; ChIP-Seq
samples were crosslinked for 5 minutes followed by 12 minutes of sonication in a covaris S220.  Lysate was then clarified followed by overnight IP for Kr-h1.  10% of lysate was saved and prepared as sonication control libraries. Libraries for sequencing were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB E7645), as described by the manufacturer but using half volumes of all reagents and starting material. For PCR amplification optimal number of PCR cycles was determined using a qPCR side-reaction using 10% of adapter-ligated, size-selected DNA. 13 cycles of PCR were used for Kr-h1 libraries and 5 cycles were used for Input controls.