GSM5470619: JVF_LSK; Mus musculus; RNA-Seq
Sorted cells were stained with Trypan blue and Countess II Automated Cell Counter (ThermoFisher) was used to assess both cell number and viability. The single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of up to 10,000 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. scRNA-seq