GSM5655657: SCAF505_11-28_KO_PBS; Mus musculus; RNA-Seq
The mouse brain was dissociated using the Adult Brain Dissociation kit (Miltenyi Biotech, Cat#130–107- 677), following by demyelination with Debris Removal Solution according to manufacturer's recommendation. For microglia isolation, the cell suspensions were labelled with CD11b microbeads (Miltenyi Biotech, Cat#130–093-634) and loaded onto MACS columns (cat#130–042-201). Single cell suspension generated from the half-brain sample were subjected to scRNA-Seq using a 10× genomics Chromium analyzer with Chromium Next GEM Single Cell 3' regent kit v3.1 at the NCI single cell analysis facility, Bethesda MD. Sequencing was performed with NCI single cell facility, using 50,000 reads per cell.