GSM6210022: Drug_rIL7, scTCR-seq [D15_IL7_TCR]; Mus; RNA-Seq
Single cell suspension was generated from the tissue following the manufacturer's recommentations (Tissue dissociation kit, mouse, Miltenyi). Total RNA capture and cDNA amplification were performed according to the manufacter's instructions (Single Cell 3' Gene Expression v3 or Single Cell 5' Immune Profiling v1.1 protocol, 10x Genomics). Briefly, the dissociated cells were loaded onto the 10x Chromium system targeting 5,000 cells (for NAÏVE, D7_PBS, D7_CT26, and D14_CT26) or 10,000 cells (for D15_PBS and D15_IL7). For each single cell, total RNA from the cell lysate contained in gel bead-in-emulsion (GEM) was captured with UMI and 10x barcoded gel bead and then reverse transcribed to cDNA. The barcoded cDNAs were pooled and amplified. Library was generated according to the manufacter's instructions (Single Cell 3' Gene Expression v3 or Single Cell 5' Immune Profiling v1.1 protocol, 10x Genomics).