GSM6171414: Soleus Male rep 1; Mus musculus; RNA-Seq
Total RNA was extracted from Soleus and TA muscles using Qiagen fibrous tissue total RNA extraction mini kit. 10ng of total RNA from the extracted samples were used in the Tag-Seq library preparation. Takara SMART-Seq Stranded protocol for Illumina sequencing platforms was used for cDNA library preparation.  libraries with concentration > 3ng/  ul and fragment size between 300-450 were sequenced using an Illumina 500 sequencer.