GSM6124244: liver_PtenE2f78TKO_2 [TKO_b2]; Mus musculus; RNA-Seq
Frozen pieces of ~100 mg liver tissue were pulverized on dry ice and RNA was extracted using Quagen's Rneasy kit. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the TruSeq PolyA kit (illumina).