GSM5009424: Adult Control Lung Epithelium; Mus musculus; RNA-Seq
Cellular suspensions were loaded on a GemCode Single Cell Instrument (10x Genomics, Pleasanton, CA) to generate single cell GEMs. Approximately ~1200 to 2800 cells were loaded per channel. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3' Gel Bead and Library Kit (now sold as P/N 120230, 120231, 120232, 10x Genomics) and scATAC scATAC sequencing library was prepared on the 10X Genomics Chromium platform following the manufacturer's protocol (10X Genomics 1000110). The isolated nuclei suspension was diluted and then incubated with transposition mix for a targeted nuclei recovery of 10,000 cells. NextSeq500 with 2 × 75 paired-end kits using the following read length: 98bp Read1, 14bp I7 Index, 8bp I5 Index and 5bp Read2.