Metabarcondig of endolithic communities: eukaryotes
The 18S region was amplified with the Euk_1391f/EukBr primers as recommended by the Earth Microbiome Project (http://www.earthmicrobiome.org). The 18S region was amplified following initial denaturation at 94 C for 3 min, 35 cycles of denaturation at 94 C for 45 s, annealing at 57 C for 60 s, extension at 72 C for 90 s, followed by a final extension at 72 C for 10 min. Amplicons, quantified by a Qubit dsDNA HS Assay Kit (Life Technologies, Carlsbad, CA, USA), were pooled and then purified with Qiagen PCR CleanUp kit (Macherey-Nagel, Hoerdt, France). Paired-end sequencing (2  300 bp) was carried out on an Illumina MiSeq platform.