GSM4891022: Mediastinal Lymph Node -  CD4+ T cells; Mus musculus; RNA-Seq
Cell suspensions were derived from lymphoid tissues by mechanical dissociation and from whole LV by Collagenase II enzyme digestion. CD4+CD3+CD4+ cells were FACS sorted  from lymphoid tissues and CD4+CD3+CD4+GFP+ cells were FACS sorted  from the  LV. 5000 purified cells from each site were transfered to isolate RNA for the construction of sequencing libraries.. All sequencing libraries were generated using protocols and reagents included in the SMARTer Mouse TCR a/b Profiling Kit (Takara Bio, Cat. 634403).