Pacific Biosciences RS II sequencing of CHEN125-Quinoa1
High-molecular weight DNA for sequencing of accession CHEN125 on the SMRT Pacific Biosciences sequencing platform was obtained from grinding freshly harvested leaves under liquid nitrogen into powder, followed by extraction of the genomic DNA using a Nucleospin XL kit. DNA from leaf material of further accessions was isolated using a streamlined CTAB protocol, followed by one step of chloroform extraction. After precipitation, DNA was dissolved in 1 x TE pH 8.0.