GSM5399084: C133S4_2; Homo sapiens; RNA-Seq
Cells were collected and lysed in RLT buffer containing beta-mercaptoethanol. RNA was isolated using the RNeasy RNA isolation kit (QIAgen) according to manufacturer's extraction. Library preparation was performed using QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen) with an input of 100 ng total RNA according to manufacturer's instructions.