GSM5114467: Stage 7 in vitro (exp22); Homo sapiens; RNA-Seq
SC-islets were incubated with 1:1 mix of TrypLe and 10x Trypsin – EDTA for 10 min in +37°C. Disassociation media was neutralized by adding 3x volume 0.5% FBS in PBS and strained through a 30-um filter to remove cell clusters. Finally, the cells were resuspended in PBS+0.04% BSA, washed, counted and diluted to a 1x10^6 cells/ml concentration for encapsulation. 10x Genomics Chromium Single Cell 3' RNAseq platform using the Chromium Next GEM Single Cell 3' Gene Expression (version 3.1 chemistry).