CITE-seq of Homo sapien: adult female mammary tumour (HER2+, ER+)
CITE-seq captures using 10X Genomics 3'v3 kit (157 TotalSeq-A Antibodies). Tumours were dissociated with miltenyi human dissociation kit, single-cell suspension were washed twice, incubated with FC block for 15 minutes then with TotalSeq-A antibodies for 30 minutes. Samples were then washed thrice, viability was checked to be over 70%, pooled in equal proportions then captured with return aim of 8000-10000 cells.