RNASEQ OF MB49-3
RNA extraction from MB49 cells was done in triplicate using the Rneasy mini kit (Qiagen). Quality-assessed RNA samples were used to generate RNA-seq libraries using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). All the libraries were sequenced on the HiSeq X Ten (Illumina) to generate 150 bp paired-end reads. Clean data were aligned to the mm10 reference genome via software Hisat2 6. Then, HTSeq 7 was used to estimate gene and isoform expression levels. Gene expression values were calculated by the RPKM (reads per kilobase per million mapped reads) method.