bacterial 16S rRNA sequencing: stool from Omegaven-TPN-fed mouse OV4
Libraries were prepared using the Metafast protocol, at Fasteris facilities (Genesupport/Fasteris SA, Plan-les-Ouates, Switzerland). This initial step is a single PCR amplification using tagged primers (for the identification of the sample) for 16S with the addition of random bases. The tagged primers allow pooling several PCR products before library preparation step. Details are provided at https://www.fasteris.com/en-us/NGS/DNA-sequencing/Metagenomics-16S-18S-ITS-amplicons