GSM5398576: F1 SMG rep3; Homo sapiens; RNA-Seq
Total RNA was isolated from the cell using RNeasy kit (Cat#74106, QIAGEN, Hilden, Germany) and purified according to the manufacturer's instructions. RNA samples were quantified using an ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and the quality was confirmed using the Experion System (Cat#7007106JA, Bio-Rad, Hercules, CA, USA). The Ion AmpliSeq Transcriptome Human Gene Expression Kit (Life Technologies) was used to construct a library. An Ion PI IC 200 Kit (Life Technologies) and an Ion PI Chip Kit v2 BC were used for sequencing, using an Ion Proton next-generation sequencer.