GSM6171117: 52303US, Matched Group 4, M.tb-uninfected, CMV-uninfected; Homo sapiens; RNA-Seq
Qiagen RNeasy kit following the manufacturer's instructions (including optional DNase digestion) and RNA was eluted twice, 30 µl per elution. Purification of mRNA, generation of double stranded cDNA and library construction were performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L) with adapters and barcode tags (dual indexing). The concentrations used to generate the multiplex pool were determined by Picogreen.