GSM5702770: 3054A PDGFB_neg; Mus musculus; RNA-Seq
Brains were explanted and tumors microdissected under epifluorescence microscope. Cells were and sorted by FACS to separate glioma fluorescent cells from surrounding, non-fluorescent cells. RNA was harvested using MinElute RNA micro kit (Qiagen). Sequencing libraries were generated using CORALL Total RNA-Seq Library Prep Kit (Lexogen) on PolyA+ RNA.