GSM5398443: J30-1; Homo sapiens; RNA-Seq
Cells were centrifuged at 300 xg for 1 min and the supernatant was replaced by 1 mL Trizol. Gently mixed cell lysate was then flash-frozen in liquid nitrogen and MagZol Reagent Kit was used for RNA extraction. VAHTS mRNA-seq V3 Library Prep Kit for Illumina was used for library construction. Sequencing was performed on Illumina NovaSeq 6000 instrument in PE150 mode.