RNA-Seq of mouse lung DCs
Flow cytometry-sorted cells were collected in RLT buffer (Qiagen; Hilden, Germany), and RNA was extracted and DNAse-treated using RNeasy Mini Kit (Qiagen) according to the manufacturers protocol. cDNA libraries were synthesized using Clontech SMARTer Stranded RNA-Seq kit (Mountain View, CA) with multiplexing primers. Quality of RNA and cDNA libraries were analyzed using 2100 BioAnalyzer (Agilent Technologies; Santa Clara, CA), then samples were sequenced with Illumina HiSeq2500 (San Diego, CA).