GSM5426212: OS17_culture_Rep1; Homo sapiens; RNA-Seq
Tumor harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). Single cell suspensions in 0.04% BSA-PBS of cell lines, and dissociated tumor tissues were generated and run on Chromium Single Cell 3′RNA-sequencing system (10x Genomics) with the Reagent Kit v3 according to the manufacturer's instructions. Libraries were constructed according to Chromium Single Cell 3′RNA-sequencing system (10x Genomics) with the Reagent Kit v3 according to the manufacturer's instructions. Briefly, cells were loaded into Chromium Single Cell Chip v3 (10x Genomics) with a targeted cell recovery of 5,000 cells per sample. After performing cDNA purification, amplification, and library construction (sample index PCR 14 cycles) as instructed, we sequenced sample libraries on a half lane of HS4000 (Illumina) to yield (after QA) about 65,000 paired-end reads per cell.