GSM6123047: colon enterocytes [mouse] - [Epcam+, Cd44-neg, Cd66a-high] - [replicate #3]; Mus musculus; RNA-Seq
Sorted cells were centrifuged to form a pellet and flash-frozen in liquid nitrogen. Total RNA was extracted using the NuceloSpin RNA XS kit (Macherey-Nagel). RNA-seq libraries were generated using the Illumina Stranded mRNA Prep kit, which employes poly-A oligonucleotides in order to "pull-down" mRNAs. RNA-seq libraries were sequenced using either an Illumina HiSeq 4000 machine (30 million, single-end, 100 bp reads) or an Illumina NovaSeq 6000 machine (20 million, paired-end, 100 bp reads). Individual samples are annotated with regard to the platform used to sequence them.