16S:Illumina MiSeq
PCR products were visualized using electrophoresis on 1.5% agarose gels and purified using VAHTSTM DNA Clean Beads (Vazyme, Nanjing, China), then quantified using a Nanodrop 2000 spectrophotometer. Finally, 10 ng of DNA from each sequencing sample was sequenced with the Illumina MiSeq platform