GSM5608023: Untreated Control HBE Donor 1328 H3K27ac ChIP [D24_UT_K27ac_repHBE1328]; Homo sapiens; ChIP-Seq
After removal of accumulated mucus with 10 mM DTT in PBS, cells were trypsinized with 0.25% trypsin-EDTA for 10 min at 37 °C and neutralized. . Cells were manually counted using a hemocytometer, and equal numbers of cells from each donor were pooled. Each pool included four samples (one from each donor and each representing a different cytokine stimulation). Manufactures instructions where follow to carry out a ChIP using Diagenoid Low Cell ChIP kit, NGS libraries where created from the purified DNA using Rubicon DNA-seq kit.