GSM5234202: lib38408: Ribo Stim IP_5; Mus musculus; OTHER
Samples were subject to either hemagluttanin IP (for RiboTag samples) or sucros fractionation (for SPEED) before RNA was isolated. Samples were either kept in -80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol. Sequencing libraries were constructed from total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara) and NexteraXT DNA sample preparation kit (Illumina) to generate Illumina- compatible barcoded libraries. cDNA libraries construction used the SMARTseq v4 Ultra-Low Input kit (Clontech)