GSM5008735: repo_Rpl10_TBI_D6_ZT00-3; Drosophila melanogaster; RNA-Seq
Adult fly heads were separated at certain ages and time points. Affinity Purification of Ribosomes and Isolation of Ribosome-Bound mRNAs were carried out as previously described (Huang, et al., 2013; Ng, et al., 2016). mRNAs were then amplified and convereted to cDNAs as previously described (Kula-Eversole, et al., 2010; Nagoshi, et al., 2010; Xu, et al., 2019). The cDNA was submitted to HGAC at the University of Chicago for library preparation and sequencing. Library construction and sequencing floowed the protocol at HGAC at University of Chicago