RNA-seq of PXO99A
Xanthomonas oryzae pv. oryzae PXO99A was grown in liquid peptone sucrose medium to reach OD600 0.6. Then the bacterial cells were collected and total RNA was extracted using RiboPure-Bacteria Kit (Thermo Fisher Scientific) following the instruction of manufacturer. The rRNA-depleted RNA was fragmented and reverse transcribed to cDNA for library construction. The cDNA library was sequenced using Illumina platform with PE150 mode.