GSM5425916: T47D_LTED_LSD1_4G4_Rep2_ATACseq; Homo sapiens; ATAC-seq
ATAC-seq experiments were performed as previously described (Corces et al., 2017) with minor modifications. 50,000 viable cells were used to perform the transposition reaction.The cleanup of the transposed fragments was performed using Zymo DNA Clean and Concentrator-5 Kit (Zymo catalog number D4014) and DNA was eluted in 21 µL of provided elution buffer The ATAC-seq library construction was performed as previously described (Corces et al, 2017). To determine the average fragment size of each library, samples were run through a high sensitivity DNA screentape (Agilent Technologies #5067–5584) following the manufacturer's instructions on an Agilent Technologies 2200 TapeStation machine. Concentration of each library was measured using Qubit dsDNA high sensitivity reagents (Thermo Fisher Scientific #Q32851) following the manufacturer's instructions on a Qubit 3 fluorometer. .