RNAseq_A549_Ctrl
Total RNA was isolated using Trizol (Invitrogen). RNA was treated with DNase (DNase-Free DNase Set, Qiagen) and repurified using the RNeasy micro plus Kit (Qiagen). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry with 1x75bp single end setup.