RNA-Seq of EC1201
Total RNA was extracted using the TRIzol reagents from fresh samples. RNA-seq libraries was constructed according to manufacturers instructions using Stranded Total RNA Sample Pre Kit (Illumina, San Diego, CA), and 500 ng total RNA was subjected to library construction using a TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA) according to the manufacturer's protocol. Sequencing was performed using the 150 bp pair-end read platform (BerryGenomics, Beijing, China).