GSM5469941: Monkey1_media_24hr; Macaca mulatta; ChIP-Seq
Cells were harvested and cross-linked in 10% Formaldehyde for 30 minutes. Cells were pelleted and stored dry at -20C for future work. Thawed pellets were sonicated using Bioruptor (Diagenode) to a fragment size of 300-500bp and then incubated O/N with CREB1 Ab. Antibody was pulled down, DNA:protein de-crosslinked and DNA purified/eluted using the commerical True MicroChIP kit (Diagenode) Libraries were prepared and indexed using the MicroPlex kit (Diagenode). 100 bp single end read sequencing was performed on the NovaSeq platform