GSM5426092: HEK293T-EBNA2-full-HiC; Homo sapiens; Hi-C
For HEK293T Hi-C, cells were crosslinked,digested and ligated. Then protein were degraded by protein K, precipitated by isopropanol. Hi-C libraries were prepared using in-situ Hi-C protocol. Dynabeads MyOne Streptavidin C1 beads was washed once with 1×Tween Washing Buffer. The beads were added to Hi-C DNA and incubated at RT for 15 min with rotation. The beads were washed by adding 200μl of 1×TWB. Heat the tubes on a Thermomixer at 55°C for 2 min with mixing. The beads were reclaimed using a magnet, supernatant was discarded. Repeat washing. End-repair and dA tailing following the in situ Hi-C protocol, adapters were added to DNA fragments. PCR amplification was performed with thirteen to nineteen cycles using Illumina primers.