GSM5234573: WT1-Retroperitoneal fat; Sus scrofa; RNA-Seq
Three adult individuals for each group  were used. Animals were humanely killed to ameliorate suffering. Upon excised from the corpse, a piece of tissue fragments from five organs including liver,longissimus dorsi,psoas major,subcutaneous fat and retroperitoneal fat were placed immediately frozen in liquid nitrogen, and then stored at −80 °C until use. Total RNA were extracted using TRIZOL reagent (Life Technologies, Beijing, China), For RNA sequencing, approximately 3 ug total RNA of each sample was used to construct transcriptome libraries following the manufacture's standard procedures.