WGS OF NORMAL
DNA was extracted from skin tissue samples of C57BL/6 mice using the DNeasy Blood and Tissue kit (Qiagen). Extracted DNA was used to generate whole-genome sequencing (WGS) libraries using Truseq Nano DNA HT Sample Prep Kit (Illumina).  The libraries were sequenced on the HiSeq X Ten (Illumina) to generate 150 bp paired-end reads. Sequencing data were mapped to the reference mouse genome (UCSC mm10) by Burrows-Wheeler Aligner (BWA) software. Then, SAMtools and Picard were used to sort BAM files and do the duplicate marking. Local realignment and base quality recalibration were performed using GATK 3. Samtools mpileup and BCFtools were used to do the variant calling and identify SNP, InDels. The somatic SNV was detected by muTect. These obtained variants were then annotated by Annovar.