GSM4903810: Rh36 cell xenograft_R42_S9_RITA treated; Homo sapiens; RNA-Seq
4 tumor samples were selected from each treatment group (16 samples in total), and total RNA extracted from each sample was isolated with E.Z.N.A. total RNA kit I (#R6834-02, Omega Bio-tek, USA) according to the manufacturer's instructions. For RNA isolation from tumor samples, small pieces of tumors (20–40 mg) were homogenized by Qiagen TissueRuptor (Germany) and proceeded further according to the manufacturer's instructions. ≈100 ng of total RNA was used to generate stranded mRNA libraries with the TruSeq®Stranded mRNA Low Throughput Sample Preparation kit according to manufacturer's instructions (#15031047, Illumina, USA). The concentration and quality of the cDNA libraries were assessed by Qubit (Life Technologies, USA) and TapeStation, respectively.