16SRNA-Seq of Eisenia fetica after feeding
The jagged ends of DNA fragment would be converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. Then add an 'A' base to each 3' end to make it easier to add adapters. After all that, fragments too short would be removed by Ampure beads.