Illumina HiSeq 2000 sequencing; RNA-seq of sexually reproducing Pseudo-nitzschia multistriata diatom cells
RNA-seq of sexually reproducing Pseudo-nitzschia multistriata diatom cells
Cultures were collected by filtration onto 1.2 µm pore-size membrane filters (RAWP04700 Millipore) RNA was extracted with Quick-RNA™ Fungal/Bacterial Miniprep Kit (Zymo research) according to manufacturer's instructions with the exception of the beads for the cell lysis that were replaced with acid washed glass beads.  Single-end (SE) libraries were prepared using a Beckman Biomek FX and the Illumina® TruSeq® Stranded Total RNA Sample Preparation kit, with poly-A selection and starting with 500 ng total RNA.