low-pass whole-genome sequencing of a circulating tumor cell: Sample 34142_034142C_341595
Circulating tumor cell (CTC) detection, immunofluorescence analysis, and single-cell copy number analysis was performed using the Epic Sciences platform (Werner et al. J Circ Biomarkers 2015, PMID 28936239). CTCs were isolated from stained glass slides and genomic material was subject to whole-genome amplification and sequenced on an Illumina NextSeq 500. The resultant data was then analyzed for copy number variation across known prostate cancer specific gene alterations (Greene et al. PLOS One 2016, PMID 27851748).