TCRA SEQUENCING OF NEIL3 TREATED T CELL
TCR-  deep sequencing was performed on CD3+ T cells sorted from splenocytes cocultured with mut-Neil3 peptide for 6 days. The DNA libraries were built with NEB Next Ultra DNA Library Prep kit (New England Biolabs) and sequenced on Illumina MiSeq platform to generate 150 bp paired-end reads. TCR- / V, D, J gene and clonotype defined according to IMGT59 37 and Igblast.