GSM4891573: MS_10; Homo sapiens; RNA-Seq
Peripheral blood mononuclear cells were isolated by centrifugation on a Lympholyte Cell separation medium (Cedarlane, Burlington, Ontario, Canada) gradient and total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Hilden, Germany). One µg of RNA was treated with 3 units of RNase R for 15 minutes at 37°C. After phenol-chloroform purification, the entire elution volume of each RNA was used for the library preparation, using the TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA). Samples were sequenced using the NextSeq 500 platform (Illumina) to obtain 150-nt-long paired-end reads.