GSM5470546: H-CN-A; Homo sapiens; RNA-Seq
RNA was extracted. Briefly, cells were lysed on ice with RNeasy lysis buffer (Qiagen GmbH) after washing with ice-cold PBS. RNA was then extracted using a spin column (Qiagen GmbH) and purified with a high-purity RNA isolation kit (Roche Diagnostics GmbH). RNA-seq library was constructed using Hieff NGSR MaxUP II DNA Library Prep Kit for Illumina (Yeasen, Shanghai, China) following manufacturer's instruction. Sequencing was performed by Novogene Biotech (Beijing, China) using HiSeq X Ten system (Illumina, San Diego, CA).PE150