NGS RNAseq of S401 pRT802-rbpA
RNA-seq library preparation was carried out by Oxford genomics centre, Wellcome trust for human genetics, UK. The Truseq Stranded mRNA library prep kit from Illumina was used to prepare the library. Total RNA was fragmented, and the first strand of cDNA was synthesised following random oligo priming. The second strand synthesis of cDNA was incorporated with dUTP. The ribosomal RNA was selectively removed using the Ribozero universal bacterial kit (NEB). Indexing adapters were added to the ends of the cDNA, and the cDNA was end repaired and uridine depleted. The samples were multiplexed, and PCR cycles were started.