GSM5702577: 1899_4; Mus musculus; RNA-Seq
Lungs were removed,Single cell suspension was sorted as DARQ7-CD31-CD45- lung cells . The sorted cells were loaded onto a Chromium Controller instrument (10× Genomics, Pleasanton, CA, USA) to generate single-cell barcoded droplets (GEMs) according to the manufacture's protocol using the 10× Single Cell 3' v1 chemistry Resulting libraries were uniquely indexed using the Chromium i7 Sample Index Kit, pooled, and sequenced were sequenced on the Illumina NovaSeq 6000 sequencer using an SP 100 cycles flow cell in a paired-end, single indexing run