Genome sequencing of soil microbes
In total, 21 DNA samples with high quality (A260/280 ranging 1.8 to 2.0) were employed for sequencing. Amplification of bacterial 16S ribosomal DNA was performed in an Eppendorf DNA thermocycler with the broadly conserved primer pair 515F/806R, which was designed for application on an Illumina platform aiming at the V4 hypervariable region of the 16S rRNA gene.