GSM5616047: Total fraction control replicate 1; Homo sapiens; OTHER
Cell lysis (QIAgen, #79306) followed by RNA precipitation and fragmentation (Covaris S220). NuGEN Ovation Library kit (Ovation Universal RNA-seq System): DNase (QIAgen) treatment was performed on column before the library preparation. cDNA fragmentation was performed using Covaris S220 with the following settings: 145 W, 10% duty cycle, 200 cycle/burst, 50 sec, water level 12. Strand selection purification was performed in a cDNA to beads ratio of 1:0.9.