GSM4903768: Scramble_1; Homo sapiens; OTHER
Ψ-seq was performed on total RNA as described (Schwartz et al., 2014). Briefly, RNA was chemically fragmented, subjected to FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific), followed by 3′ ligation of an RNA adaptor using T4 ligase (New England Biolabs). Ligated RNA was reverse transcribed, and cDNA was subjected to 3′ ligation with a second adaptor using T4 ligase. The single-stranded cDNA product was amplified for 9-12 cycles in a PCR reaction. Libraries were sequenced on Illumina NextSeq500 with paired end reads (38 bp for read1 and 28 bp for read2).