GSM5656138: Liver Biopsy (P05SN); Homo sapiens; RNA-Seq
Liver Biopsy: RNA samples from cryopreserved liver biopsies were prepared by TRIzol extraction (Thermo Fisher), and the quantity, purity, and integrity of the samples were measured with Nanodrop (Thermo Scientific) and Bioanalyzer (Agilent).PBMC: Whole blood from patient was collected in Tempus Blood RNA tubes (Applied Biosystems) and stored in -80oC. RNA was extracted using the Tempusä Spin RNA Isolation Kit (Invitrogen). In short, stabilised blood was transferred to a 50 ml tube, and diluted with PBS. After vortexing, the tube was then centrifuged and the pellet resuspended. Resuspended RNA was transferred into purification filter, then purified and eluted using a microcentrifuge. RNA libraries were prepared and amplified using TruSeq Stranded mRNA sample preparation with the corresponding kit (Illumina Inc, USA). Paired-end 101 bp sequencing were done reaching approximately a total of 50 milions of reads by sample.