Amelas_ONT4
DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. Additional purification steps were performed using AMPure XP beads (Beckman Coulter). Library preparation and sequencing was performed using Oxford Nanopore Ligation Sequencing Kit SQK-LSK109 according to manufacturer's instructions. At each step, DNA was quantified using the Qubit dsDNA HS Assay Kit (Life Technologies). GDNA was purified then sheared at 20 kb using the megaruptor system (diagenode). A DNA damage repair step was then performed on 2 g of sheared gDNA and an END-repair and dA tail was performed on double stranded DNA fragments. Adapters were ligated and the library was loaded at a concentration of 0.05 pmol and sequenced for 64 h on a GridION R9.41RevD flowcell.