GSM5608449: hot1-4 Heat rep2; Arabidopsis thaliana; RNA-Seq
whole plant seedlings were harvested and flash-frozen on liquid nitrogen. Total RNA was extracted using a RNeasy Plant Mini kit (Qiagen, 74904). All samples were treated with DNase I using a RNase-Free DNase Set (Qiagen 79254) and quantified. 7 µg total RNA was used for rRNA depletion with RiboMinus (Invitrogen, A10838-08) according to the manufacturer's instructions. 100 ng of rRNA-depleted RNA was used for library preparation using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7420S) according to the manufacturer's protocol.