GSM4890908: Mixl1 induced D1Dox rep 2 ATAC-seq; Mus musculus; ATAC-seq
50,000 cells per replicate were lysed in 10 mM Tris·Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630. The chromatin within intact nuclei were digested using Tn5 transposase. Digested chromatin was retrieved using MinElute PCR Purification Kit (Qiagen), as per manufacturer specifications. Eluted chromatin was bacoded and adapter tagged using Nextera compatible sequences. 11 cycles of PCR were used to amplify the library, after which, it was purified with 1.3× and 0.5× SPRI bead selection.