GSM6209358: Primary Rat Hepatocytes Fig4_D16_50nM_rep2; Rattus norvegicus; RNA-Seq
Rat livers collected at the end of the toxicity studies were snap-frozen, powdered, and RNA was extracted with the miRNeasy kit (Qiagen). HepG2.2.15 RNA was isolated using the ThermoFisher RNAqueous-96 Total RNA Isolation Kit, as per the protocol. cDNA libraries were prepared with the TruSeq Stranded Total RNA Library Prep Kit (Illumina) and sequenced on a NextSeq500 sequencer (Illumina), all according to manufacturers' instructions.