Biofilm metagenome
The quantity of DNA samples was estimated using a NanoDrop ND-1000. DNA purity was tested by agarose gel electrophoresis and on an Agilent 2200 TapeStation instrument. DNA samples were used to sequence preparation applied by the NEBNext Ultra II Library Prep Kit.The metagenome sequencing was performed by Illumina with MiSeq/NextSeq chemistry (MiSeq Reagent kit v2/ NextSeq 500/550 High Output Kit v2.5). Galaxy Europe server was employed to pre-process the raw sequences (i.e., sequence filtering, mapping, quality checking). Low-quality reads were filtered by Prinseq (min. length: 100; min. score: 15; quality score threshold to trim positions: 20; sliding window used to calculated quality score:1). Filtered sequences were checked with FastQC. The filtered sequences produced by Prinseq were co-assembled with Megahit