GSM5703125: VEH1; Mus musculus; RNA-Seq
Liver tissues were flash frozen on dry ice. RNA was extracted using the Zymo Quick-RNA Miniprep Kit (Cat# D7001, Zymo Research, Irvine, CA), followed by quantification with Nanodrop. The RNA quality control was determined using Agilent RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA) and the Bioanalyzer (Agilent Technologies, Santa Clara, CA UAS). A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.