GSM6209741: RNA_LckKmt2d_WT_CD8SP_Biol rep2; Mus musculus; RNA-Seq
RNA was isolated following the manufacturer's instructions of the Monarch RNA Isolation Kit (New England Biolabs; #T2010S). RIN scores and concentration were performed using High Sensitivity RNA ScreenTape (Agilent Technologies; #5067-5579/#5190-6507) and run using the Agilent Technology software with an electronic ladder. Quality RIN scores above 7 were used for RNA-Seq library preparation. Library creation was perfromed using KAPA Library Quantification Kit for Illumina (KAPA; #KK4824). Quality checks on size and concentration of libraries was performd using the High Sensitivity D1000 DNA Kit on BioAnalyzer (Agilent; #5067-4626/#5190-6502). Paired-end 50-bp reads were obtained for pooled libraries using Illumina Novaseq 6000.