MiSeq of water bacterial communities: Microplastics from environment and microcosm
Partial V3-V4 16S rRNA libraries, were conducted by following the protocol described in the metagenomics sequencing Library guide (Illumina, CA, USA). Briefly, the library preparation protocol entailed a first amplicon PCR step, followed by amplicon purification, and then the addition of barcodes. Sequences were demultiplexed and depleted of Illumina indexes and primers.