GSM4891014: Young diploid hepatocytes_rep1; Mus musculus; RNA-Seq
Primary hepatocytes were isolated by a two-step collagenase perfusion method, and sorted based on their reporter expressions and DNA content determined by Hoechst intensities. RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using SMART-Seq v4 Ultra Low Input RNA Kit (Takara, Cat#634894) according to the manufacturer's protocol.