C6-biological repetition-3
To characterize the transcriptional profile of EcN and EcN C6, triplicate cultures of each strain were incubated overnight in LB broth at 37 . Overnight cultures were then diluted 100-fold in 4 mL LB broth and incubated for 2.5 hours. RNA was extracted using TRIzol reagent as described by manufacturers protocol (Invitrogen, USA). The rRNA in extracted RNA was removed by Ribo-off rRNA Depletion Kit (Vazyme, China). RNA library was constructed using a NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (NEB, USA) and was sequenced by Illumina HiSeq X Ten platform.