GSM5607963: Untreated Control HBE Donor 1243 [UT_1]; Homo sapiens; RNA-Seq
After removal of accumulated mucus with 10 mM DTT in PBS, cells were trypsinized with 0.25% trypsin-EDTA for 10 min at 37 °C and neutralized. . Cells were manually counted using a hemocytometer, and equal numbers of cells from each donor were pooled. Each pool included four samples (one from each donor and each representing a different cytokine stimulation). Manufacturer's instructions for Illumina TruSeq stranded mRNA