Secondary WW filtered effluent treated after ozonation
To determine the taxonomic information for each remaining sequence, the sequences must be run through USEARCH Global Search along with a python script to determine the taxonomic assignment that is assigned to each read. The search will find the top six matches , then a confidence value is assigned to each taxonomic level by taking the number of taxonomic matches that agree with the top match and then divide by the number of total matches. Then, the data goes into the diversity analysis program. This program takes the OTU table output from sequence clustering along with the output generated during taxonomic identification and begins the process of generating a new OTU table with the taxa information tied to each cluster.