GSM6210024: Drug_rIL7, Visium [D15_IL7_Visium]; Mus; OTHER
Total RNA capture from the tissue and cDNA amplification were performed according to the manufacter's instructions (Spatial 3' v1 protocol, 10x Genomics). Briefly, the OCT embedded tissues were cryosectioned to a thickness of 10um. The cryosectioned tissues were H&E stained on a Visium Spatial Gene Expression Slide, then followed by permeablilization, reverse transcription, second strand synthesis, denaturation, and cDNA amplification. Library was generated according to the manufacter's instructions (Spatial 3' v1 protocol, 10x Genomics).