UCE sequences of  Cladius_difformis_USNMENT01322223
Samples were extracted destructively or non-destructively using Qiagen DNeasy extraction kits. DNA was sheared to 400-600 bp using a QSonica Q800R sonicator. Genomic libraries were prepared using Kapa Hyper Prep Kits (1/4 volume reactions) and iTru, 8bp dual-indexing adapters (Glenn et al. 2019). Samples were pooled into pools of 8-10 samples and enriched for ultraconserved elements (UCEs) using custom-designed probes (MYcroarray, Inc., now ArborBiosciences, Ann Arbor, MI) targeting 2590 UCE loci in Hymenoptera (myBaits UCE Hymenoptera 2.5Kv2P, Branstetter et al. (2017)). Enriched pools were pooled together (up to 100 individual samples) and shipped to the University of Utah genomics core facility for sequencing on an Illumina HiSeq 2500 instrument (2x125 bp v4 chemistry). Submitted here are raw, untrimmed sequencing reads. For trimming raw reads, use i7:GATCGGAAGAGCACACGTCTGAACTCCAGTCAC*ATCTCGTATGCCGTCTTCTGCTTG and i5:AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT*GTGTAGATCTCGGTGGTCGCCGTATCATT, where * indicates the index sequence. Adapter (index) sequences are specified as "i7-iTru-i7_i5-iTru-i5 in the sequence file names.