GSM6171741: C2C12, Control, Input; Mus musculus; RIP-Seq
Cells were fixed with 0.1% formaldehyde for 10 min at room temperature. Cells added 1.25M glycine solution for 5min. RIP-seq libraries were prepared using VAHTS Small RNA Library Prep Kit for Illumina. 1 μg of total RNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 300-400bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.