GSM5763734: Th1_60hr; Mus musculus; RNA-Seq
In vitro cultured T cells were collected at each time point into RLT-plus buffer, and RNA was extracted with RNeasy Plus Mini Kit (Qiagen). Extracted RNA was further used for cDNA amplification with SMARTA-Seq2 for 12 cycles. Sequencing libraries were prepared with fragmented cDNA amplification products using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with 2 x 150 paired-end reads.