GSM5425940: T47D_LTED_LSD1_KO_2E8_K4me1_CUT_and_RUN; Homo sapiens; OTHER
CUT&RUN was performed with CUTANATM ChIC/CUT&RUN kit according to the manufacturer's protocol (EpiCypher, 14-1048). Briefly, 500,000 cells were gently harvested with a cell scraper (Corning, 3008) and bound to activated Concanavalin A beads. The cells bound to the beads were incubated with antibodies at 4℃ overnight. After extensive washing, cells were incubated with pAG-MNase (EpiCypher, 15-1016) for 10 min at room temperature and then chromatin digestion was performed with the chromatin digestion buffer for 2h at 4°C. After chromatin digestion, the stop buffer and E. coli spike-in DNA (EpiCypher, 18-1401) were added and chromatin fragments were purified with DNA cleanup columns. Libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7370L) and sequenced on Illumina NovaSeq 6000 (Illumina).