GSM6124201: hESC-derived xEM cells by BMP4/FGF2 differentiation on soft substrates; Homo sapiens; RNA-Seq
The samples were dissociated with Accutase for 15-30 min at 37 °C until they completely dissociated into single cells assessed by light microscopy. The dissociation solution was thoroughly washed, then samples were loaded for capture onthe 10x Chromium platform using the Single Cell 3′ v3 reagents. cDNA was synthesized and amplified according to the manufacturer's instructions (10X Genomics). The resulting libraries were sequenced on the Nova-seq platform. 10x genomics v3 10x genomics v3