GSM5764497: hs_Sub2_H3K4me3_glut; Homo sapiens; ChIP-Seq
Fat biospsies were collected and SVF were isolated by centrifugation. For ATAC-seq, nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina).DNA was purified with MinElute PCR Purification Kit (Qiagen). For ChIP-seq, Lysates were clarified from sonicated nuclei and histone/CTCF or RNAPII-DNA complexes were isolated with antibody. ATAC-seq, ChIP-seq and ChIRP-seq libraries were prepared and sequenced using standard Illumina protocols for HiSeq 2500 instrument. ATAC-seq, ChIP-seq, ChIRP-seq