RNA-Seq of Temnothorax unifasciatus Worker
RNA extraction of all samples was conducted in September 2017 using the Nucleo-Spin Mini kit (Macherey-Nagel). For each species, we analyzed a pool of 6 workers and another pool of three queens. We extracted RNA from whole bodies, as we had no prior assumptions as to which tissue differed most strongly in gene expression between our groups.  Samples were shipped to StarSEQ (Mainz) for library preparation and 100bp paired-end sequencing on an Illumina HiSeq. In total, we obtained 15 million reads on average per sample.