Hotspot screening in K562 replicate 2 Reference
1. infected with pgRNA library lentiviruses at an MOI of <0.3 (1000-1500x coverage of the library).2. 72 hours after infection, EGFP+ cells were selected by FACS (Day-0).3. genomic DNA extraction, PCR amplification of sgRNA-coding sequence and high-throughput sequencing.