TCRA SEQUENCING OF CONREOL T CELL
TCR-  deep sequencing was performed on CD3+ T cells sorted from splenocytes. The DNA libraries were built with NEB Next Ultra DNA Library Prep kit (New England Biolabs) and sequenced on Illumina MiSeq platform to generate 150 bp paired-end reads. TCR- / V, D, J gene and clonotype defined according to IMGT59 37 and Igblast.