GSM5917154: RRBS_AML patient UPN01 at relapse (post transpantation); Homo sapiens; Bisulfite-Seq
Genomic DNA was extracted using the Wizard SV Genomic DNA purification system, following manufacturer's instruction and including a step of protein lysis using Proteinase K. Genomic DNA was digested using methylation-insensitive restriction enzymes to generate short fragments enriched for CpG dinucleotides at the extremities. After end-repair, A-tailing and ligation to methylated adapters, the CpG-rich DNA fragments were subjected to size selection, bisulfite conversion, and PCR amplification.