RNA-seq of Homo sapiens;Fresh frozen: Sample A20-BRAIN-MET_ROi
Tumor tissue was disrupted using roto-stator homogenization. RNA was isolated using the RNeasy Mini Kit (QUIAGEN) according to manufacturer protocol. RNA was quantified using a NanoDrop® Spectrophotometer,  and the quality of RNA was  then  analyzed  via  2100 Bioanalyzer Instrumentwhich  using the  RNA 6000 Nano  assay (Agilent)  for determination  of  an  RNA  Integrity  Number  (RIN).   Briefly, 1 µg of total RNA was converted to RNAseq libraries using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina) and sequenced on an Illumina HiSeq 2000 or HiSeq2500 using a 2x50bp configuration