GSM5008991: Upp5_R2; Marchantia polymorpha; RNA-Seq
50 mg 15d thalli of WT, Mpbmi1/1l, Mph2a;H2AK115R/K116R and Mph2a;H2AK119R mutants were used for RNA extraction. RNA was extracted using the MagMAX™ Plant RNA Isolation Kit (Thermo Fisher Scientific) in biological triplicates. Libraries were generated using DNA-free RNA with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina according to manufacturer's instructions. Sequencing was performed on an Illumina HiSeqX in 150-bp pair-end mode at Novogene (Hong Kong).