GSM4891333: Calvaria Tmem53+/- rep3; Mus musculus; RNA-Seq
The dissected calvaria were frozen in liquid nitrogen and crashed by a cryopress (Microtec). The homogenized tissue powders were dissolved in ISOGEN solution (Nippon Gene) followed by total RNA extraction using Qiagen RNeasy kit (Qiagen). rRNA was removed by Ribo-ZeroTM Magnetic Gold Kit (Illumina). The cDNA was synthesized by NEBNext Ultra Directional RNA Library Prep Kit for Illumina R (New England biolabs).