GSM5399454: mice ID: EP92 [unstimulated]; Mus musculus; ChIP-Seq
ChIP was performed as described previously: Carey MF, Peterson CL, and Smale ST. Chromatin immunoprecipitation (ChIP). Cold Spring Harbor protocols. 2009;2009(9):pdb.prot5279. Libraries are prepared using the Illumina TruSeq ChIP Sample Preparation Kit. After quantification and profiling ChIP DNA >5 ng ChIP-enriched fragmented input DNA is used to repair the ends of fragments. After adenylation of the 3' ends indexed paired-end adapters are ligated. After purification of ligated products and PCR amplification a QC is performed in order to normalize and pool the indexed libraries. The indexed libraries are the sequenced as single read 1x100 bp fragments on the HiSeq-2500.