GSM5009055: IND-PALF_CC-14; Homo sapiens; RNA-Seq
Total RNA was extracted from archived frozen liver tissue using a TissueLyser LT and the RNeasy® Mini Kit protocol (QIAGEN, Hilden, Germany). RNA purity and concentration were confirmed using the NanoDrop 2000™ Spectrophotometer (Thermo Scientific, DE, USA). RNA quantity was determined with Qubit fluorometer. Total RNA samples were checked for quality based on fragment size using Agilent Bioanalyzer 2100. RNA libraries were prepared for sequencing using standard Illumina protocols