GSM5425959: RNA-seq T47D T0 Rep2; Homo sapiens; RNA-Seq
RNA was extracted from cells treated or not for 6hs with 50pM or 10nM  R5020 and submitted to massive sequencing using the Solexa Genome Analyzer. RNA was isolated from cells with TRIzol reagent (Ambion), ethanol precipitated, and  dissolved in sterile water. RNA concentration was measured with a Qubit fluorometer  and RNA subjected to Bioanalyzer for quality control. Libraries were prepared using 1 μg of polyA+ RNA by PCR amplification of cDNA with bar-coded primers using the Illumina  TruSeq kit at the CRG Genomic Facility. Libraries were sequenced using Illumina HIseq-  2500 to obtain pair-ended (PE) 100-base-long reads. ChIP assays were performed as described (Strutt and Paro, 1999)