GSM5657416: ChIPseq noAB WT Rep D; Cereibacter sphaeroides 2.4.1; ChIP-Seq
Cells lysed and DNA sheared with sonication, DNA-protein fragments trimmed with micrococal nuclease and Rnase treated, no antibody was added before proceeding as before ChIP-seq library preparation and sequencing was performed at GeneWiz. Libraries for sequencing were created using the Illumina TruSeq ChIP library preparation kit (Illumina) following the standard protocol. ChIP-seq libraries were sequenced on an Illumina HiSeq 3000 2x150bp reads using the standard protocol.