Control Female 2
Sequin RNA Mix (Hardwick et al. 2016) sequencing control was added at a fraction of 1% of total purified mRNA. Purified mRNA with Sequin was used to prepare libraries using the KAPA Stranded RNA-Seq Library Preparation Kit (Roche, CA, USA) with SeqCap Adapters (Roche CA, USA) as per manufacturers instructions. The libraries were sequenced on an Illumina NextSeq 500 System.