RNA-seq of human ESC-derived cortical neuron: 30 DIV control
The mRNA fraction was selected from the total RNA before conversion to cDNA. Second strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and QCed before paired end sequencing over seven lanes of a flow cell.