GSM4891145: anti-PD1_1 YUMM 2.1; Mus musculus; RNA-Seq
Total RNA was extracted and amplified from CD45+ sorted samples using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara). The RNA Integrity Number of each sample was determined using the 2100 Bioanalyzer instrument (Agilent Technologies). Samples were selected with stringent criteria based on RNA Integrity Number of 7.0 or greater, and a minimum of 500 ng of total RNA. Total RNA was subjected to ribosomal RNA depletion and further used as input for library construction by the Nextera XT DNA Library Preparation Kit (Illumina). RNA libraries were prepared for sequencing using standard Illumina protocols.