GSM5702438: SP-C C121G Day 7; Mus musculus; RNA-Seq
To enrich for EPCAM+ cells, a single cell suspension was prepared by physical and enzymatic dissociation (Collagenase, DNase, Dispase) followed by MACS sorting by LS columns with CD45+ cell removal using CD45 micobeads followed by EPCAM+ cell enrichment using EPCAM microbeads. libraries were prepared for sequencing using standard Illumina protocols