WGS of SARS-CoV-2 variant from clinical sample
SARS-CoV-2 extraction was performed using the MagMAXTM Viral/Pathogen Nucleic Acid Isolation Kit (Cat# A42352) from Applied Biosystems (ThermoFisher Scientific). Viral RNA extraction was performed following manufacturer's protocol, using 400 ul of sample transport media. RNA was eluted in 55 ul of which 25 ul was loaded onto the sequencing instrument. Sequencing was performed using the Ion AmpliSeq SARS CoV 2 Research Panel for Genexus 6.2 from ThermoFisher Scientific following manufacturer's instructions.