GSM5469875: D3 BRA CHIR FGF RNA-seq biological replicate 3 (EXP5); Mus musculus; RNA-Seq
Total RNA was extracted from cells using a QIAGEN RNeasy kit in RLT buffer, following the manufacturer's instructions. Extracts were digested with DNase I to eliminate genomic DNA. The total RNA samples were quality controlled to determine RNA integrity and concentration using Agilent Tapestation prior to library preparation. Libraries were prepared using the KAPA mRNA HyperPrep kit (Roche) and sequenced as 76bp single-end, stand-specific reads on the Illumina HiSeq 4000 platform (Francis Crick Institute).