GSM6210175: C2C12 cells , ATF3; Mus musculus; ChIP-Seq
10 μg of antibodies against Atf3 (Santa Cruz Biotechnology), or normal mouse IgG (Santa Cruz Biotechnology) was used for immunoprecipitation. Immunoprecipitated genomic DNA was resuspended in 20 μl of water. For DNA library construction，a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer's instructions. Bioanalyzer analysis and qPCR were used to measure the quality of DNA libraries including the DNA size and purity. Finally, DNA libraries were sequenced on the Illumina Genome Analyzer II platform.