RNA-Seq of Eimeria stiedai: Gametophytes
RNA samples from Ou, Os, Mz4, and Gc samples were used for Illumina (San Diego, CA, USA) library construction and sequencing, respectively. The cDNA libraries for Illumina HiSeq X Ten sequencing were constructed as follows: mRNA was enriched from the total RNA using the magneticoligodTbead binding method and sheared into short fragments using fragmentation buffer. Then, the short mRNAs were used as templates to synthesize double-stranded cDNAs using random primers by reverse transcription. The cDNA fragments were purified using a QiaQuick PCR extraction kit (Qiagen, Venlo, Netherlands) and ligated with Illumina sequencing adapters. The ligation products were size selected by agarose gel electrophoresis and enriched by PCR to construct the cDNA libraries, which were sequenced on the Illumina HiSeq X Ten platform