Ion Torrent sequencing; MDA-MB-468 aspirin 1 hour 0.5 mM (biol repl 2)
Total RNA was extracted from cell lines using the PureLink RNA Mini Kit (Invitrogen) according to manufacturers instructions. RNA (10 ng) was reverse transcribed using SuperScriptTM IV VILOTM Master Mix with ezDNaseTM Enzyme (Invitrogen), as per manufacturers recommendations. Construction of libraries was carried out using Ion AmpliseqTM Library Kit Plus (Ion Torrent) with ERCC RNA Spike-in Mix 1 (Invitrogen). A custom Ion AmpliSeq RNA Panel (Ion Torrent) was used to generate 66 amplicons representing unique targeted genes.