RRBS of human CLL sample: Sample GCLL-0325-T-01
RRBS libraries were generated from 50 ng input DNA using the Tecan Ovation RRBS Methyl-Seq System following the manufacturer's recommendation except that we performed scaled-down half reactions from restriction digest through final repair after adapter ligation. Libraries were PCR amplified for 12 cycles. Paired-end 100-base reads were generated on Illumina HiSeq2500 using custom primers (Tecan) without adding a PhiX spike-in. Adapter sequences were removed using a custom script available from Tecan