GSM5113929: eluturation-arrest-release-exp191107_medium-00min_bio-rep2; Saccharomyces cerevisiae; RNA-Seq
cell pellets were lysed in 300 µL TRI Reagent (Zymo Research) by bead beating using a Fastprep 24 (4°C, settings: 5.0 m/s, 1 x 30 seconds). Cell debris was pelleted (13 krpm, 5 minutes) and the supernatant recovered. RNA was then extracted using the direct-zol RNA microprep kit (Zymo Research). mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and NEBNext Ultra II RNA Library Prep Kit for Illumina® (NEB, #E7775) was then used to prepare libraries for paired-end (2x150 bp) Illumina sequencing