GSM5398797: single-cell RNA seq data of a patient with HLA-B27neg anterior uveitis , patient#b27neg2; Homo sapiens; RNA-Seq
AqH (100-150µl) was collected from each study subject using a 30G needle under local anesthesia, and immediately shipped at 4°C to the department of neurology at the University Clinic Muenster (Germany) for scRNA-seq and/or flow cytometry analysis. Freshly isolated cells were centrifuged once, counted and up to 5,000 of the input cells were used for scRNA-seq. Freshly isolated single cell suspensions were loaded onto the Chromium Single Cell Controller using the Chromium Single Cell 3' Library & Gel Bead Kit v2 or v3 chemistry (both from 10X Genomics). Sample processing and library preparation was performed according to manufacturer's instructions using AMPure XP beads (Beckman Coulter). Sequencing was either carried out on a local Illumina Nextseq 500 using the High-Out 75 cycle kit with a 26-8-0-57 read setup or commercially (Microanaly, China) on a NovaSeq 6000 using the 300 cycle kit with paired end 150 read setup.