GSM6122345: RNAseq, polyA, lib6, pollen, Col0, rep2; Arabidopsis thaliana; RNA-Seq
Mature pollen was harvested with vacuum method. RNA was extracted with RNeasy mini kit (Qiagen) with in column DNAse I digestion 300-500 ng of RNA was used to prepare libraries using the Illumina TruSeq Stranded mRNA Library Prep protocol, following manufacturer's instructions.