GSM5589457: ATACseq_Neurons_b2_TKO_CrebKO_2; Mus musculus; ATAC-seq
ATAC-seq was performed according to the previously described protocol for Omni-ATAC (Corces et al. 2017). Briefly, 50,000 cells were washed with cold PBS and resuspended in lysis buffer to extract nuclei. The nuclei were cold-centrifuged at 500 x g for 10 min. The nuclei pellet was incubated with transposition reaction buffer for 30 min. at 37 °C. The DNA was purified using the PCR Purification Kit (Qiagen). The eluted transposed DNA was submitted to the PCR using Q5 High-Fidelity Polymerase (NEB). DNA was amplified with 11-12 cycles of PCR.