WGS of MRSA: male pus (from bone)
The library preparation protocol includes tagmentation of genomic DNA, library amplification, library clean up, library normalization and library pooling. Library denaturation and dilution were performed, and then diluted PhiX control V3 was spiked into the libraries to obtain 1% PhiX as an external control. The prepared library pool was loaded into the reagent cartridge and then sequenced by 150 bp paired-end NextSeq 500 sequencing (Illumina, USA).