Illumina HiSeq 2500 paired end sequencing; Whole exome sequencing of DNA purified from colorectal tumors induced by 1% DSS and adjacent colon controls in MLH1-KO mice
Whole exome sequencing of DNA purified from colorectal tumors induced by 1% DSS and adjacent colon controls in MLH1-KO mice
Colons were extracted, cut longitudinally and washed with cold PBS. Tumors were excised with scissors and homogenized with a razor blade. Tumorigenesis is induced in MLH1-KO mice by giving drinking water with 1% DSS for 1 week. DNeasy DNA isolation protocol for animal tissue. Illumina paired-end Whole exome capture library preparation using full-length index adapters and KAPA DNA polymerase. DNA is fragmented before library construction and Clusters are generated before sequencing.