GSM5703185: ob 0.5 mg/kg rep3; Mus musculus; RNA-Seq
Total RNA was isolated from the snap frozen kidney samples using the TRizol kit. The samples were then quantified and qualified by Agilent 2100 Bioanalyzer, NanoDrop  and 1% agrose gel. 1 μg total RNA with RIN value above 6.5 was used for following library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols