GSM5962467: NPp53T; Mus musculus; RNA-Seq
Prostate tissue was dissected in PBS, minced with scissors, enzymatically digested with papain and filtered through a 20-mm cell strainer (PluriSelect). Cell suspensions of approximately 6000 cells with >90% viability were loaded onto a 10X Genomics Chromium Controller (10x Genomics) using a Chromium Next GEM Single Cell 3ʹ Kit v3.1 (PN-1000269) and prepared for next-generation sequencing following the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide. Libraries were sequenced using a NextSeq High-Output 150 cycle kit (Illumina Inc.) following the 10x Genomics User Guide recommendations.  Each library produced approximately 200 million paired-end reads.