GBS of Pisum Sativum (pea) of six RIL populations
Pea leaf green tissues for DNA extraction were collected, flash frozen in liquid nitrogen and stored at -80C before analyses. Genomic DNA was extracted from 6 bulked plants per genotype using the DNeasy Plant Mini Kit (Qiagen) and checked for integrity on 1% agarose gel. DNA quantitation was performed by means of the Quant-iTTM PicoGreen dsDNA assay kit (Life Technologies, P7589). 100 ng of genomic DNA were used, 3.6 ng of total adapters were used, the genomic DNAs were restricted with ApeKI enzyme, and the library was amplified with 14 PCR cycles.