LSC1
Total RNA of cellular  samples were isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions. The concentrations of RNA were quantied using Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA). RNA purity and integrity were checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA) and the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively. Ribosomal RNA (rRNA) was removed by Epicentre Ribo-zero rRNA Removal Kit (Epicentre, USA). rRNA free residue was cleaned up by ethanol precipitation. Then, RNA-seq libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA). The libraries were sequenced on the Illumina Hiseq 4000 platform with 150 bp paired-end reads.