reactor2day020
2 mL of sludge were sampled at different times across the 161 days of digesters operation (days 8, 20, 30, 34, 69, 83, 114, 125, 128, 135, 140, 161) and stored at -70 C. Metagenomic DNA was extracted using FastDNA Spin kit for soil (MP Biomedicals, USA)