GSM6206677: RNA-seq for WT skeletal muscle stem cells 1; Mus musculus; RNA-Seq
Total RNAs from cells or tissues were extracted using TRIzol reagent (Invitrogen, 15596018) according to the manufacturer's instructions. cDNAs were synthesized under the manufacturer's instructions of HiScript® II Reverse Transcriptase Kit with gDNA wiper (Vazyme, R223-01). Real-time PCR was performed to quantify the expression level of mRNAs by Luna Universal qPCR Master Mix (NEB, M3003E) and LightCycler 480 Real-Time PCR System (Roche). GAPDH (glyceraldehydes 3-phosphate dehydrogenase) or 18s RNA were used as internal controls for normalization.