GSM4890923: L1 line without expression of Mn-superoxide dismutase and deficient production of H2O2. - L1_rep1; Nicotiana tabacum; Bisulfite-Seq
Genomic DNA was extracted from 200 mg of leaf tissue of each sample, which was pulverised with liquid nitrogen. Extractions were carried out by the cetyltrimethylammonium bromide (CTAB) method described by Clarke 2009 The library preparation was done by BGI according to their standard directional WGBS pipeline. The WGBS consisted of a 150 pb paired-end sequencing with coverage of 12X on a NovaSeq 6000 system (Illumina, Inc.).