GSM6209155: HSJ019, K27M-KO, replicate 3; Homo sapiens; RNA-Seq
The concentration of the single-cell suspension was assessed with a Trypan blue count. Approximately 5000 cells per sample were loaded on the Chromium Single Cell 3′ system (10X Genomics). GEM-RT, DynaBeads cleanup, PCR amplification and SPRIselect beads cleanup were performed using Chromium Single Cell 3′ Gel The concentration of the single-cell suspension was assessed with a Trypan blue count. Approximately 5000 cells per sample were loaded on the Chromium Single Cell 3′ system (10X Genomics). GEM-RT, DynaBeads cleanup, PCR amplification and SPRIselect beads cleanup were performed using Chromium Single Cell 3′ Gel Bead kit Indexed single-cell libraries were generated using the Chromium Single Cell 3′ Library kit and the Chromium i7 Multiplex kit