GSM5657376: RNAseq D56A Rep D; Cereibacter sphaeroides 2.4.1; RNA-Seq
Hot acid phenol:chloroform extraction, NaOAc/isopropanol precipitation, ethanol wash, Dnase treatment, Qiagen Rneasy kit clean-up and concentration RNA-seq library preparation and sequencing was performed at the Joint Genome Institute. Libraries for sequencing were created using the Illumina TruSeq Stranded Total RNA kit (Illumina) following the standard protocol. RNA-seq libraries were sequenced on an Illumina NextSeq in 2x151 reads using the standard protocol.