title: WGS of Lepomis macrochirus: male
design: We extracted DNA from the 25 mg subsample of gill tissue using a Qiagen DNeasy extraction kit, and we quantified extracted DNA using a fluorometer. After quantification, we sheared 650 ng DNA to a modal size of 500 - 600bp using a sonicator, and we input 250 ng of sheared DNA to a commercial library preparation kit (Kapa HyperPrep; F. Hoffmann-La Roche AG) following the PCR-free protocol to incorporate unique dual index adapters.