title: GSM5963888: SRC_3KO_GFP_P2_S68; Mus musculus; RNA-Seq
library_construction_protocol: Tregs were isolated from the spleens of SRC-3KO and WT animals and sorted for GFP. Total RNA was isolated from GFP+ Tregs using Qiagen micro RNAesy plus kit. Quality control of isolated RNA samples was checked using Agilent Bioanalyzer. Takera SMART-Seq v4 kit was used to generate the low throughput sequencing libraries. RNA libraries were prepared using Takara Bio SMART-seq v4 plus by manufacturer recommended protocol.