title: Illumina NovaSeq 6000 sequencing; 3' UTR RNA-sequencing of humanized EGFR-mutant PC9 cell line xenografts treated with osimertinib/vehicle of RIG-I agonist IVT4/unspecific control IVT-GAC
design: 3' UTR RNA-sequencing of humanized EGFR-mutant PC9 cell line xenografts treated with osimertinib/vehicle of RIG-I agonist IVT4/unspecific control IVT-GAC
library_construction_protocol: Mice were inoculated with xenotransplanted PC9 cell line and with PBMCs of healthy donors. At tumorvolumes between 150 and 200mm3 treatment with osimertinib or vehicle p.o. was started. After a run-in period osimertinib/vehicle were stopped and IVT4 or IVT-GAC were injected i.t.. RNA was extracted with RNeasy kits. Sequencing libraries were prepared using the QuantSeq 3' mRNA-Seq library kit (Lexogen, Austria)