title: GSM5918325: leaf-pooled RNA-seq; Medicago sativa; RNA-Seq
library_construction_protocol: The RNA was extracted from these plants using a Qiagen RNeasy Plant Mini kit according to the manufacturer's instructions, pooled and used to construct libraries For the RNA-seq, the libraries were validated using a High Sensitivity Chip on the Agilent 2100 Bioanalyzer. The samples were barcoded, multiplexed and sequenced in an Illumina HiSeq 2000 machine using paired-end reads with 150 cycles. The cDNA library preparation and sequencing reactions were conducted in the Biomedical Genomics Center, University of Minnesota.