title: GSM5471896: OB_WT2; Mus musculus; RNA-Seq
library_construction_protocol: The olfactory bulb was collected from AAV-GFAP-iCre-P2a-TurboRFP injected Sox9fl/fl mice or C57BL/6J mice 4 weeks after viral injection. Bulbs were dissected and dissociated, and astrocytes were isolated using FACS directly into QIAGEN RLT lysis buffer (Cat# 74004) with 1% ß-Mercaptoethanol. Bulk RNA was isolated using the RNeasy Micro Kit (74004, QIAGEN). Illumina sequencing libraries were constructed from 10ng of total RNA using the Trio RNA-Seq System (0507-96, NuGEN).