title: RNA seq of Plasmodium
design: Parasites were harvested for RNA isolation after 6 hours of stress induction. Total RNA was isolated using TRIzol reagent according to the protocol. DNAse treated RNA was used for cDNA synthesis. Quality of the RNA was verified using Agilent Bioanalyzer 2100. Three biological replicates were pooled together for performing RNA sequencing. The cDNA libraries were prepared for samples using Illumina TruSeq RNA library preparation kit. Transcriptome sequencing was performed using Illumina NextSeq 500 system (1x150 bp read length) at BioServe Biotechnologies (India) Pvt Ltd. Hyderabad in replicate.