title: DMS of NEB 5-alpha F'Iq: Single-codon substitutions in AadB, sequencing run 6, generation 0
design: A library of single-codon substitutions in AadB was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We plasmid prepped uninduced, exponentially-growing cultures of the library mixed with wildtype AadB and determined the allele frequencies at the start of the growth experiment as our generation 0, replica 2 sample. Data for codons 1-178 was used from these files. This was used as Replica 2 for regions 1 (codons 1-89) and 2 (codons 90-178).