title: GSM5473869: muscle stem cells, wild-type, rep 2 [S2]; Mus musculus; RNA-Seq
library_construction_protocol: Skeletal muscle was dissected and digested with Collagenase II and Dispse at 37C for 30 minutes. Mononucleated cells were collected through a 70micron cell strainer. MuSCs were FACS purified as CD34+, CD31/CD45/DAPI/Sca1- populations. Total RNA was purified using the RNeasy Micro Kit (Qiagen #74004). Libraries were constructed using the SMARTer stranded pico input total RNA-seq kit (Takara, #63411) following manufacturer instructions.