title: GSM5590457: single_cell_Queen_rep1; Apis mellifera; RNA-Seq
library_construction_protocol: For single cell libraries, cell suspension was collected after the whole brain was dissected. For bulk  libraries,  Five adult brains were pooled and homogenized in Trizol reagent to extract the total RNA. For single cell libraries, single-cell 3' gene expression libraries were generated following the manufacturer's instructions (10X Genomics#1000121, Chromium Next GEM Single Cell 3' Reagent Kits v3.1). For bulk  libraries, the sequencing libraries was  were generated following the manufacturer's instructions  VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme#NR605).