title: GSM4903943: xPhf8 rep2; Xenopus tropicalis; RNA-Seq
library_construction_protocol: RNA extraction was performed usingTRIzol (Ambion) and phenol/chloroform extraction. RNA clean-up and DNA digestion were performed with RNeasy Mini-Kit (Qiagen).  RNA quality was checked with an Agilent Bioanalyzer 2100 using Agilent RNA 6000 Pico Kit. rRNA was depleted using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Illumina). rRNA depleted RNa was cleaned up using RNA Clean & ConcentratorTM-5 (Zymo Research). Library preparation was performed using NEBNext UltraTM Directional RNA Library Prep Kit for Illumina (New England Biolabs) with NEBNext Multiplex Oligos for Illumina primer sets 1 and 2. DNA purification and size selection was performed using Agencourt AMPure XP (Beckman Coulter). Quality and size of the libraries were verified on the Agilent Bioanalyzer using Agilent DNA 1000 Kit.