title: GSM5859088: RNA_552_r2_POLE4-KO_exp; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from 5*10^6 cells using RNeasy Plus Mini Kit (Qiagen) and DNA was eliminated by treatment with the RNase-Free DNase Set (Qiagen). RNA was treated with the NEBNext rRNA Depletion kit (NEB #E6310) and strand-specific RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (NEB #E7420). NEBNext Ultra Directional RNA Library Prep kit (NEB #E7420) following the manufacturer's protocol.