title: difenoconazole_T0
design: Metataxomic analysis of the soil fungal microbiota was performed by analysing the Internal Transcribed Spacer (ITS2). The ITS libraries were obtained with the use of the following primers: 5'GCATCGATGAAGAACGCAGC (ITS3 forward, without overhangs) and 5' TCCTCCGCTTATTGATATGC (ITS4 reverse, without overhangs) according to White et al.22 (1990). The methodology for library preparation followed the protocol available from Illumina Support Center (ISC) with a minor modification (2Phanta Max Master Mix, Vazyme Biotech, Nanjing City, China was applied instead of Kapa Hifi Hot Start Ready mix).