title: 16S-DNA-Seq:Spillar_Ranch+Substrate+Wild+March+NONE
design: samples were collected and genomic DNA extracted in triplets using DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) following the manufacturers instructions. The ITS region was PCR amplified using Illumina adaptor ligated universal primers 5ITS region ITS7(5-GTGAATCATCGAATCTTTG-3) and ITS4(5-TCCTCCGCTTATTGATATGC-3) from each triplet. The thermocycler condition was maintained at 94oC for 3 min, followed by 25 cycles of 94oC for 30 s, 55oC for 30 s, 72oC for 30 s and a final extension of 72oC for 3 min.