title: GSM5590310: R691_20Weeks_Rectal_Biopsy; Macaca mulatta; RNA-Seq
library_construction_protocol: 10mL of whole peripheral blood (collected in PAXgene tubes) and Rectal biopsies were collected at weeks 20 and 29, and cryopreserved at -80C. The PreAnalytix PAXgene Blood RNA or Qiagen's RNeasy kits were used to eextract RNA and an RNA-integrity number of 8 or above (measured using a bioanalyzer) was ensured prior to library preparation. Library preparation was performed using the TruSeq Stranded Total RNA Library Prep Kit after rRNA and globin mRNA removal using the Globin-Zero Gold rRNA removal kit. ~30 million paired-end reads obtained per sample