title: ancestral_2_RNAseq
design: Overnight cultures grown in LB of the ancestral strain and amp D8 cultures were prepared in biological triplicate, then pelleted. Cell pellets were frozen at -80C and sent to Quintara Biosciences (Cambridge, MA) for RNA extraction and sequencing. RNA was extracted using the RNeasy Mini Kit (Qiagen), then sequenced on a NovaSeq S4 flow cell, with 2x150 paired-end reads at 100X coverage.