title: RNA-Seq of Mus Musculus: adult male VSMC
design: Total RNA was extracted with the Direct-zol RNA MiniPrep Plus w/ TRI Reagent Kit (Zymo Research) according to the manufacturers instructions. DNase I treated RNA analysed on the Tape Station 4200 (Agilent) showed an RNA integrity number (RIN) of 8.79.8. SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio) were used for cDNA amplification. Indexed sequencing RNA Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian according to manufacturer's instruction (Illumina, San Diego, US).