title: GSM5011739: SKOV3_PDE10Ako_5H5; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extracted with TRIzol and treated with TURBO DNA-free kit as per manufacturer's instructions. RNA quality was assessed using the Agilent 2100 Bioanalyzer.  RNA with a RNA Integrity Number (RIN) of ≥7.0 was used for sequencing library preparation. RNA passing quality control was converted to a sequencing ready library using the NEBNext Ultra II Directional RNA library kit as per the manufacturer's instructions (NEB, Ipswich, MA). The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems, Woburn, MA) prior to cluster generation.  Cluster generation was performed according to the manufacturers recommendations for onboard clustering (Illumina, San Diego, CA).  We generated between 30-35 million paired end 75bp sequencing reads per sample for transcript level abundance.