title: GSM5471358: CVB5_RD; Homo sapiens; ncRNA-Seq
library_construction_protocol: At 24 h post-infection (hpi) , discard the cell supernatant and wash with PBS, then collect cell pellets, total RNA were extracted from cells using RNAiso Plus according to the manufacturer's instructions. Ribosomal RNA was first eliminated, and first strand cDNA and second strand cDNA were synthesized using random hexamer primers ; followed by end repair, poly-A 3' ends, ligation of adapters, and screening of DNA fragments in order to construct the RNA-seq library.