title: GSM5116889: Neuron-ENR5; Mus musculus; Bisulfite-Seq
library_construction_protocol: Isolation of cells from the hippocampus by FACS for Nestin-Gfp (NSPCs; nestin::Gfp mice), Dcx-Gfp (lPCs; dcx::Gfp) or Neuro-Fluor. Genomic DNA was isolated using the QIAamp DNA Micro Kit (QIAGEN) following the manufacturer's manuals. RRBS libraries were prepared using the Premium RRBS Kit (Diagenode) and purified twice using Agencourt AMPure XP beads (Beckman Coulter; 1X bead volume). Quality and concentration of RRBS libraries were determined using the High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical) and a fragment analyzer with capillary size of 33 cm (Advanced Analytical).