title: GSM5795896: whisker pad e12.5 4C-Seq Lef1; Mus musculus; OTHER
library_construction_protocol: Whisker pads or adult kidney were removed, digested to single cells and processed with the 4C-Seq protocol. For each sample, we harvested at least 1×107 cells and obtained 7-10 μg of output double-digested, double-ligated DNA. NlaIII and DpnII were used as primary and secondary cutter respectively; ligation was performed by using the Concentrated T4 DNA ligase from Promega. The DNA libraries were prepared for sequencing using standard Illumina protocols