title: RNA-seq of wt Arabidopsis rosette
design: Total RNA was isolated from 6-week-old rosette leaves and bolting flower buds of Col-0  with PureZol reagent (BioRad). 250 ng of Turbo DNase (Ambion) treated RNA was reverse transcribed using an Illumina TruSeq Stranded library preparation kit. The sequencing run (150bp, paired ends) was performed on an Illumina HiSeq4000 sequencer by Novogene.