title: PE250 metagenome of soil
design: V4 of 16S rRNA gene. Primers 515F-806R Dual indexed Illumina compatible libraries produced in a single step. Primary PCR performed using fusion primers Fluidigm CS oligos at their 5' ends. PCR products were normalized using Invitrogen SequalPrep DNA normalization plates. The pooled samples were sequenced using a Illumina MiSeq flow cell (v2) and a 500 cycle reagent kit (2 x 250 bp paired end reads).