title: RNA-Seq of mus musculus: old male Hippocampus
design: For RNA-seq, total RNA was isolated using TRIzol reagent (Life Technologies) according to the manufacturers instructions and libraries were constructed with the TruSeq Stranded Total RNA LT Sample Prep Kit. The Illumina NovaSeq 6000 generated raw images utilizing sequencing control software for system control and base calling through an integrated primary analysis software called Real Time Analysis.