title: GSM6068125: CB3; Mus musculus; RNA-Seq
library_construction_protocol: mRNA from mouse liver samples was extracted and enriched using oligo(dT) beads followed by rRNA removal using the Ribo-Zero kit. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer (5X) (New England BioLabs).  First strand cDNA was synthesized using random hexamer primer and M-MuL V Reverse Transcriptase (RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Final libraries quantity was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples were performed on a cBot Cluster Generation System using PEE Cluster Kit cBot-HS (Illumina, California, USA) according to the manufacturer's instructions.