title: GSM5860849: Pituitary_Ctrl P1; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was isolated using Kit from Qiagen and stored at -80 ℃. The initial reverse transcription and template switching were done using 4 µL total RNA with 2.5 ng/µL. 50 ng of ds cDNA was used to build the sequencing libraries by tagmentation with the Illumina Nextera XT Kit. Library was sequenced using an Illumina NovaSeq 6000 sequencing system. 3'Bulk RNA Barcoding sequencing