title: Streptomyces sp. UKAQ_23 Genome sequencing and assembly
design: DNA Extraction, Library preparation and Sequencing: Bacterial DNA was extracted using Mobio genomic DNA extraction kit using overnight grown culture. The quality and quantity of the extracted DNA were checked and measured on an agarose gel (0.8%) and Qubit dsDNA HS assay kit, respectively. The DNA fragmentation and library construction were done using Nextera DNA Flex Library preparation kit (Illumina), following manufacture protocol. After library construction, dual index adapters were ligated at the blunt of the DNA fragments, followed by purification using manufacture protocol. The quality and quantity of the fragment library was estimated and checked using the Qubit dsDNA HS assay kit and Agilent 2200 Tapestation, respectively. The good quality library was normalized, pooled and subsequently sequenced using 2x250 bp chemistry in Illumina MiSeq platform (Illumina Inc., San Diego, CA, USA). 5% PhiX spikein was done during library loading in the sequencing cartage.