title: GSM5939661: 1047_C1D1; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh-frozen biopsies from metastatic lesions were obtained in the dose-expansion cohort on day 1 and day 15 of cycle 1. RNA was isolated using the RNeasy Mini Kit (QIAgen AllPrep DNA/RNA/miRNA) following the manufacturer's protocol. RNA expression analyses were performed by RNA sequencing on an Illumina HiSeq 4000, with a single read consisting of 50 bp and 100 million reads per sample. Data were quality-controlled using FastQC. Reads were aligned to the human reference genome (NCBI37/hg19) using STAR v2.7.1 and annotated with Gencode v32