title: ITS1 of ctl rep.2
design: The protocol for preparing the libraries was developed on the basis of the following protocol '16S Metagenomic Sequencing Library Preparation. Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System using sequences of the specific primers: ITS1 FI2 and 5.8S with an additional adapter sequence. Indexing PCR was performed with primers containing indexing tags (Nexter XT Index Kit v2). Both PCR reactions were performed using Q5 Hot Start High-Fidelity 2X Master Mix with reaction conditions according to the manufacturer's recommendations. Then NGS sequencing using the MiSeq instrument and paired-end technology (2250 bases) and the MiSeq Reagent Kit v2 was performed according to the manufacturer's recommendations.