title: GSM5470801: End1/E6E7 cells, L2-infected rep1; Homo sapiens; RNA-Seq
library_construction_protocol: End1s were harvested for Total RNA at 24 hpi using TRIzol (Thermo Fisher Scientific 15596026) and the DNA-free DNA removal kit (Thermo Fisher Scientific AM1906), according to manufacturers' protocols. Extracted RNA was subsequently enriched for polyadenylated mRNA transcripts using the NucleoTrap mRNA enrichment kit (Macherey-Nagel), again according to the manufacturer's protocol. For the comparison of Mock- and Ct serovar L2-infected transcriptomes, RNA samples were subsequently assayed for fragmentation using an Agilent 5200 Fragment Analyzer; intact samples were enriched for polyadenylated transcripts via the NucleoTrap kit a second time due to the high incidence of ribosomal RNA in the fragment analysis results. cDNA library preparation was performed using the Ion Xpress Plus Fragment Library kit (Thermo Fisher Scientific 4471269), according to the manufacturer's protocol.