title: Amplification of16SrRNA gene of marine bacteria from chemostat experiment to investigate degradation of permafrost
design: Bacterial DNA was extracted from filters using the Qiagen Power Soil kit. Amplification of the 16S V3-V4 regions was performed using the sequence specific regions (341F-805R) described in (Herlemann et al., 2011) using a two-step dual-index PCR approach specifically designed for Illumina instruments.