title: GSM5963767: mAktKO 2; Mus musculus; RNA-Seq
library_construction_protocol: Extensor digitorum longus was removed and frozen in liquid nitrogen, and RNA was harvested using RNeasy Mini Kit after incubation with Proteinase K with 1 ug of total RNA for the construction of sequencing libraries. Libraries were prepared by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760) according to manufacturer's instruction