title: Genomic sequence of Homo sapiens: adult female patients tissues generated patient-derived xenograph
design: Massively parallel sequencing was performed on DNA from cell lines and PDX models using a custom designed panel of 157 genes known to be implicated in HGSOC and EMCA tumors56. 500ng of genomic DNA was sheared randomly into 200 base pair fragments with the CovarisTM LE220 Focused-UltraSonicator (Covaris, Woburn, MA). Sheared DNA was A-tailed and ligated with adaptor-embedded indexes using the NEBNext UltraTM DNA Library Prep Kit for Illumina (New England BioLabs, Inc., Ipswich, MA). DNA quality, fragment size, and concentration of library preps were measured using Agilents DNA 1000 chips in conjunction with the 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). High quality samples were pooled and hybridized to a custom capture library using Agilent SureSelect kits. Hybridization pools were assessed for fragment length and concentration using the Qubit 2.0 fluorometer (Life Technologies, Carlsbad, CA) and the Bioanalyzer.  Sequencing was performed on the HiSeq 4000 (Illumina) at the University of Pennsylvania Next Generation Sequencing Core.