title: GSM5939505: OUD blood rRNA removed RNA-seq H3; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted from the blood using the PAXgene® Blood RNA Kit (QIAGEN, Hombrechtikon, Switzerland) according to the handbook's instructions For the rRNA removed library, a total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Ribosomal RNA was removed by Epicentre Ribo-zeroTM rRNA Removal Kit (Epicentre, USA). For the small RNA library, a total of 2 μg total RNA isolated from each sample using NEBNext® Multiplex Small RNA Library Prep Set For Illumina® (NEB, USA) according to the manufacturer's instruction.