title: GSM5657977: #2 iAM-1 D9, P10, untransduced, n=1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was collected using the RNeasy Mini Kit. The quality of the RNA samples designated for RNA-Seq was confirmed using the Advanced Analytical Fragment Analyzer. cDNA was generated from total RNA samples with RNA integrity numbers of 10 using the NEBNext Ultra Directional RNA library preparation method including polyA enrichment (New England Biolabs, cat# E7760S/L). Sequencing was performed on a NovaSeq 6000 system (Illumina, San Diego, CA). For each sample at least 40 million 150-bp paired-end reads were obtained.