title: GSM5471700: batch C, negative control, 1x 50 nM (C11); Mus musculus; RNA-Seq
library_construction_protocol: After restimulation with α-CD3 for 4 h, RNA was collected using the Rneasy Plus RNA extraction kit, including a gDNA elimination step. The libraries were prepared following Illumina TruSeq stranded mRNA protocol. The quality of the RNA and final libraries was determined using an Agilent 4200 TapeStation System. The libraries were pooled equimolecularly and sequenced in an Illumina NovaSeq sequencer (single-end 100 bp) with a depth of around 20 Mio reads per sample. Illumina TruSeq stranded mRNA protocol