title: Raw sequencing data for cell-free DNA from human plasma samples made via double-stranded library preparation method
design: cfDNA were extracted from plasma samples and eluted in molecular water. Libraries were made using KAPA Hyperprep kit following manufacturer's protocol. Each purified adaptor-ligated library was amplified 10-16 cycles using 1x KAPA HiFi HotStart Ready Mix (Roche) and Truseq dual-index primers and subsequently cleaned using 1.5 volumes of AMPure XP beads.