title: Whole-Exome of human Triple-Negative Primary Myelofibrosis Sample 016PMF_Lymph
design: gDNA from patients was fragmented (200-500bp) by sonication and used to prepare NGS libraries with the SureSelectXT HS Target Enrichment System (Agilent Technologies), following manufacturer instructions. The exome enrichment was performed using OneSeq 300kb CNV BackBone+Human All ExonV7 (Agilent Technologies) probes, following the standard protocol. Genomic libraries were purified with AMPure XP beads (Beckman) and sequenced according to the Illumina protocol paired-end 2x150bp.