title: GSM5983603: brl3-2 primary root tips 2h Zeocin Rep1 [brl3_Zeo_1_resequencing]; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA was extracted from root tip tissue with the Maxwell RSC Plant RNA Kit (Promega) using the Maxwell RSC instrument (Promega) accroding to the manufacturer recommendations.  Quality of RNA was checked with Bioanalyzer. All samples had a RNA Integrity Number (RIN) > 7. TruSeq Stranded mRNA Sample Prep kit. Further purification steps with 0.81X Agencourt AMPure XP beads.