title: GSM5963656: TA+/- PD-1-CD8+ T; Mus musculus; RNA-Seq
library_construction_protocol: For bulk RNA sequencing, total RNA was extracted utilizing RNA iso Plus (TaKaRa, 9109) from liver tissues or 5×10^5 CD8+ T cells that were isolated using a FACS Aria II cell sorter For single cell RNA-seq and BCR-seq, CD45+ cells from dnTGFβRII, Aire-/-, dnTGFβRII Aire+/- and dnTGFβRII Aire -/- mice (each sample mixed with hepatic cells from 2-3 mice) were sorted using the FACS Aria II cell sorter and dispensed into individual microwell plates following the manufacturer's instruction of BD Rhapsody system,The transcriptome  was split to construct the TCR library and the targeted mRNA library; For bulk RNA-seq, RNA libraries were prepared for sequencing using standard Illumina protocols or BGI protocols.