title: GSM5470876: Hippocampus, Gulf War toxicants, 4; Mus musculus; RNA-Seq
library_construction_protocol: Hippocampi were immediately dissected, flash frozen, stored at -70°, RNA was extracted with TRIzol followed by Rneasy cleanup. Poly(A) selection was performed with oligo d(T) magnetic beads. NEBNext Ultra RNA Library Prepr Kit for Illumina followed by purification with AMPure XP beads. Equimolar amounts of barcoded libraries were pooled and sequenced with an Illumina NextSeq 500 Sequencing System with a 1x75 configuration.