title: GSM5471770: KPC-Vps4bKO_rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the Qiagen RNeasy Kit, DNA was extracted using the Qiagen Maxi Cell and Tissue Kit. RNA libraries were prepared for sequencing using standard Illumina protocols, for CRISPR screen standard PCR of the sgRNA cassette with Illumina adapters was done