title: GSM5471277: WT CD8 T cell Pre-transplant Rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with the Qiagen miRNeasy Mini kit. RNA quality and quantity were assessed with the Agilent 2100 Bioanalyzer. cDNA was prepared with the SMARTseq HT Kit (Takara Bio), using 1ng total RNA. Dual indexed sequencing libraries were prepared using the Nextera XT DNA Library Prep kit (Illumina), using 250pg cDNA. Illumina TruSeq