title: GSM5765305: ChIPseq_SKNO1_shDpf2_Input; Homo sapiens; ChIP-Seq
library_construction_protocol: 10 million SKNO1 cells carrying shLuciferase or shDpf2#2487 were grown in the presence of doxycycline for 7 days to induce Dpf2 depletion. At day 7 of culture, cells were crosslinked with 1% formaldehyde for 15 minutes, quenched with 125mM Glycine for 5 minutes, washed in PBS and flash frozen at -80C. Crosslinked cell pellets were processed for ChIPseq following a previously described protocol (Boulay G et al., Cell 2017). ChIPseq libraries were generated using NEBNExt Ultra II DNA library prep kit for Illumina (NEB, E7370L).