title: Illumina HiSeq 2500 paired end sequencing; Bulk RNA-seq of Fibroblastic Reticular Cells
design: Bulk RNA-seq of Fibroblastic Reticular Cells
library_construction_protocol: Grem1 positive and negative FRCs (between 30,000 to 1,00,000 cells) (CD45- Ter119- MADCAM1- CD21/35- CD31- PDPN+) from skin draining lymph nodes of Grem1-Cre-ERT2 .cki; Rosa26-LSL-EYFP mice were sorted by FACS.  The samples were directly sorted into TRIZOL (Invitrogen) and RNA was isolated at Expression Analysis, Inc. Paired-end RNA-seq libraries were constructed using SMARTer Low Input RNA Kit (Takara).