title: GSM5939614: TAM-A33; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from descending thoracic aortaes through the phenol-chloroform method using TRIzol (Ambion) reagent. The resulting RNA quality was determined using a Fragment Analyzer Instrument and all samples had RNA integrity numbers above 7.4. RNA sequencing libraries were prepared according to Illumina's protocols using the Illumina Illumina TruSeq Stranded mRNA kit (ref #20020595). Eleven cycles of PCR were applied to amplify the libraries. Library quality was assessed using a Fragment Analyzer Instrument and libraries were quantified by QPCR using the kit KAPA KK4824, on the QuantStudio 6 of Thermo Fisher Scientific. Sequencing was performed in paired-end (2x150 pb) on an Illumina NovaSeq sequencer at the Integragen company platform