title: RNA-Seq of Pseudomonas aeruginosa: PA14 control Rep1
design: P. aeruginosa UCBPP-PA14 treated at mid-exponential phase with pine honey (PH) for 45 min at 0.5x MIC. Total RNA (3 replicates for pine honey treated and control samples) was isolated using a NucleoSpin RNA isolation kit and DNA removed with DNase I, according to the manufacturers protocol. Ribosomal RNA was depleted using the NEBNext Bacterial rRNA removal kit and the library was prepared using the TruSeq stranded total RNA kit. Samples were run on the Illumina NovaSeq6000 platform (1X150 paired-end reads).