title: GSM5473811: Rgnavus_Preg_rep2; [Ruminococcus] gnavus; RNA-Seq
library_construction_protocol: Bacterial cultures were then stabilized with RNAprotect Bacteria Reagent, then enzymatic lysis (15mg/ml lysozyme) and proteinaseK digestion were performed following manufacturers' guidelines. RNA was then extracted using RNeasy Mini Kit and on-column DNase treatment was performed following manufacturers' guidelines. RNA integrity was checked by agarose gel electrophoresis. Illumina Stranded Total RNA with Ribo Zero Plus  was employed with IDT® for Illumina® RNA UD Indexes Set A,  for cDNA synthesis and addition of barcode sequences. Sequencing of the libraries was performed using the NextSeq 500 with the NextSeq 500/550 High Output Kit v2 (75 cycles; Illumina).