title: 16S rRNA sequencing of sulphate-reducing and sulphide-oxidising bacterial communities in the hybrid LFCR and the effect of temperature
design: 16S rRNA amplicon gene sequences were analysed using the Divisive Amplicon Denoising Algorithm 2 (DADA2) pipeline as described by Callahan et al. (2016). Briefly, the raw sequencing data were filtered; the adapter, barcode and primer sequences were removed; and the ends of amplicon sequences with high numbers of errors were trimmed. The amplicons were denoised based on a model of the sequencing errors and paired-ends were merged. Chimaeras were removed and sequences clustered into Amplicon Sequence Variants (ASVs).  Taxonomy assignment of resulting ASVs were performed using the SILVA reference database (v132) (Quast et al., 2013).