title: 160-gene panel of homo sapiens:tumor of retinoblastoma  patient 64
design: Enrichment of target regions was carried outwith Human Comprehensive Cancer Panel - NGHS-501X (Qiagen) kit according to theinstructions of GeneRead DNAseq Targeted Panels V2 (Qiagen) protocol. Enrichmentreactions amplified coding regions of 160 genes by four multiplex PCRs. Librarieswere prepared in a single step with the QIAseq 1-Step Amplicon Library (Qiagen) kitand subsequently PCR-enriched using reagents from this kit.