title: River biome
design: The DNA of sample water was extracted and the 16S rRNA gene were amplified. Purified ampliconswere pooled in equimolarandpaired-endsequenced on an Illumina MiSeq platform. The taxonomy of each 16S rRNA gene sequence was analyzed by RDP Classifier algorithm  against the Silva (SSU123) 16S rRNA database using confidence threshold of 75%.