title: Sanger sequencing of Toxoplasma gondii: PCR products of sCas9 targeted act1 gene
design: Induction of sCas9 in Toxoplasma gondii RHsCas9_act1gRNA strain. Extraction of genomic DNA. PCR of the targeted gene and ligation into pGEM plasmids. Isolation of colonies and sent for sequencing with primer T7