title: ControlS4_All_microbes
design: Genomic DNA were extracted from soil or washed PBSA. For 16S, Forward primer  name 515F Forward primer sequence (5' to 3') GTGCCAGCMGCCGCGGTAA   Reverse primer name 806R Reverse primer sequence (5' to 3') GGACTACHVGGGTWTCTAAT  were used. For ITS, Forward primer  name fITS7 Forward primer sequence (5' to 3') GTGARTCATCGAATCTTTG   Reverse primer name ITS4 Reverse primer sequence (5' to 3') TCCTCCGCTTATTGATATGC  were used. Illumina adapter overhang nucleotide sequences were added to the primer. The PCR reaction was performed.