title: Nanopore MinION sequencing of Clostridium botulinum strain 1344-1-77 isolated from fecal sample from 1977 infant botulism case
design: Strain grown anaerobically for 24h at 35C in 25 mL TPGY broth. Whole culture was pelleted and resuspended in 0.5 mL of GPB with 0.5 mL of 2x Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment. DNA was processed (SPRI selection >1500bp), prepared with rapid barcoding sequencing kit (SQK-RBK-004) and 1D MinION sequenced using FLO-MIN106 R9.4 flowcell.