title: Full-length transcripts of gill, muscle, heart and intestinal: adult femlae Portunus trituberculatus
design: The purified mRNA was incubated and then used in turn to synthesize first- and second-strand cDNA. Subsequently, A-Tailing Control and Ligation Control were applied to A-tailing and adapter ligation of the double stranded cDNA, respectively. All cDNA fragments were enriched to complete the libraries construction. After the libraries were diluted to 10 pM, Agilent 2100 Bioanalyzer was used for quantitative analysis, and the qualified libraries were sequenced on an Illumina HiSeq 2500 across one lane with paired-end 150 bp.