title: GSM5827661: 1 hour rep 1; Populus tremula x Populus alba; RNA-Seq
library_construction_protocol: RNA extraction was performed as described previously (Chang et al. 1993). Libraries were prepared using 0.5 μg of RNA in the NEBNext® UltraTM Directional RNA Library Prep Kit following the supplier's instructions (New England Biolabs, Ipswich, MA, USA). For each library, reads obtained after sequencing with two Illumina HiSeq3000 (2×100 cycles) and one Miseq were used for the gene expression analyses.