title: RNA-seq of gut microbiota: adult female feces
design: The Ion 16S  Metagenomics Kit was used for rapid analyses of polybacterial samples using Ion Torrent  sequencing technology. The kit includes two primer sets that selectively amplify the corresponding hypervariable regions of the 16S region in bacteria: Primer set V-2-4-8 and set V3-6,7-9.