title: GSM5963914: SHEP MYCN-ER shSEC DMSO rep2; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq, total RNA was extracted using Trizol. For RNA-seq, Total RNA was extracted from 50 million SHEP MYCN-ER cells spiked-in with 15% Drosophila S2 cells using Trizol reagent. mRNA was purified from 1 ug total RNA with the VAHTS mRNA capture beads, and fragmented to 200-500 bp size long in 2× ProtoScript II buffer. First- and second-strand DNA synthesis and adaptor ligation were conducteded using NEB Next Ultra RNA library prep kit for Illumina. The resulting strand-specific RNA-Seq libraries were subjected to 150 bp paired-end sequencing on a NovaSeq 6000.