title: sRNA-Seq of Fusarium graminearum axenic
design: RNA enriched for the sRNA fraction was purified from fungal samples using the mirVana miRNA Isolation Kit (Life Technologies). Indexed sRNA libraries were constructed from these enriched sRNA fractions with the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturers instructions. Indexed sRNA libraries were pooled and sequenced on the NextSeq 500 platform and the sequences sorted into individual datasets based on the unique indices of each sRNA library.