title: GSM4903950: LacZ rep2; Xenopus tropicalis; RNA-Seq
library_construction_protocol: RNA extraction was performed usingTRIzol (Ambion) and phenol/chloroform extraction. RNA clean-up and DNA digestion were performed with RNeasy Mini-Kit (Qiagen).  RNA integrity quality was analysed using an Agilent 2100 Bioanalyzer. Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (Human/Mouse/Rat). Total stranded RNA sequencing libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions