title: RNA-seq of Triticum aestivum inflorescence
design: Inflorescence sections (apical, central basal) were dissected into 96-well plates containing 10 uL of RLT plus (Qiagen, Germany) and cDNA was prepared using the G&T-seq method as previously described (Macaulay et al. 2016).  After quality control and quantification, cDNA was normalised to 0.2 ng/ul before Nextera (Illumina, San Diego, USA) libraryrary preparation using a Mosquito HV liquid handler (STP, Royston, UK) in a total reaction volume of 4 ul as described in Mora-Castilla et al, 2016. libraryraries were pooled by volume and sequenced on a single lane of a NovaSeq 6000.