title: GSM5472098: ATAC_HUDEP2_Control_Rep2; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 undifferentiated HUDEP-2 cells were washed with cold PBS and lysed in a modified Omni-ATAC-seq lysis buffer (10mM Tris-HCl, pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Tween-20 and 0.01% Digitonin) for 3 min. The lysates were washed 3 times with buffer (10mM Tris-HCl, pH 7.5, 10mM NaCl, 3mM MgCl2 and 0.1% Tween-20) and spin at 500g for 10min to harvest the nuclei. Transposition reaction was performed using Illumina Tagment DNA Enzyme and Buffer (Cat# 20034197) in the presence of 0.1% Tween-20 and 0.01% Digitonin. DNA was isolated using Qiagen MinElute Reaction Cleanup Kit. Sequencing libraries were generated by using NEB High Fidelity 2x PCR Master Mix and amplified for 9 cycles using the following cycles: (1) 72°C, 5 min (2) 98°C, 30 sec (3) 98°C, 10 sec (4) 63°C, 30 sec (5) 72°C, 1 min (6) Repeat steps 3-5, 4x (7) Hold at 4°C. The amplified DNA fragments were cleaned and size-selected by SPRIselect beads. The libraries were pair-end (2x50bp) sequenced on Illumina Nextseq  platform