title: GSM5471063: WT iPSC-derived neurons A6 differentiation1 rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was collected from 2 million purified neurons for each sample. Purification of total RNA was completed using the PureLink RNA Mini Kit (Thermo Fisher 12183018A). Assessment of purified RNA was completed using a NanoDrop. Final RNA quantification was completed using the Quant-iT RNA assay (Invitrogen) and RNA integrity analysis was completed using a fragment analyzer (Advanced Analytical). Library preparation was completed using the TruSeq Stranded mRNA kit (Illumina RS-122-2103) per manufacturer instructions.