title: WGS of Cryptoccous
design: Sequencing library is generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, England) and following manufactures recommendations. The genomic DNA is randomly fragmented to a size of 350bp by Bioruptor, then DNA fragments are narrowly size selected with sample purification beads. The selected fragments are then end polished, A-tailed, and ligated with the full-length adapter. After these treatments, these fragments are filtered with beads again. At last, the library is analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.