title: WGS: Urine sample 4462
design: Urine samples were removed from the -80C freezer and thawed to room temperature. DNA was extracted using the Norgen Urine DNA Isolation Kit following the manufacturers protocol with one exception. We started with 500uL of urine (rather than 1.75 mL) and adjusted the volume of the Binding Solution used accordingly. DNA was then sent to MiGS for library preparation and sequencing. Sample libraries wereprepared using theIllumina DNA Prep kit and IDT 10bp UDI indices, and sequenced on an Illumina NextSeq 2000,producing 2x151bp reads. Demultiplexing, quality control and adapter trimming was performed with bcl-convert (v3.9.3).