title: RNA-Seq of FxBN_F1: 34mo control quadriceps
design: RNA isolation from powdered quadriceps using Trizol followed by Qiagen RNAeasy. 200 ng of total RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster, CA). mRNA sequencing was performed using the Illumina HiSeq 2000 system .