title: GSM6065799: M2 HLF, Control, Polysome mRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs or mRNAs from monosome & polysome fractions of HLF cells were extracted and purified with TRIzol reagents, chloroform and isopropanol. 2 µg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using QuantSeq 3' mRNA-Seq FWD library prep kits for Illumina.