title: GSM5918020: Mic60 KO cells, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol following manufacturer's instructions. RNA was treated with Turbo DNA-free kit (life technologies) and quality checked with TapeStation (Agilent). Sequencing library preparation was then completed using the QuantSeq 3'-mRNA kit (Lexogen) to generate Illumina-compatible sequencing libraries according to the manufacturer's instructions.