title: GSM5827318: Chronic_E1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Plus Micro kit (Qiagen) according to the manufacturer's protocol. Reverse transcription was performed for both qPCR and sequencing libraries using a mixture of random hexamers, dNTPs, M-MLV buffer, RibolockTMRNase Inhibitor, and RevertAidTM M-MuLV Reverse Transcriptase (Fermantas). Sequencing libraries were generated using a Nextera XT Sample Prep Kit according to the manufacturer's protocol. RNAseq. Single-read. Libraries were sequenced using Illumina NextSeq500 using one 75 bp high output flow cell.