title: GSM5470852: T2375_PS40: Saliva - Never-Smoker (64); human saliva metagenome; RNA-Seq
library_construction_protocol: BAL and Saliva was stored in RNAlater solution at -80°C. Samples were thawed and spun to pellet cells, and supernatants were removed. RNA was extracted using QIAzol lysis reagent and miRNeasy Mini kit (Cat. # 217004). 200 ng total RNA was used to construct sequencing libraries Total RNA was treated with Illumina TruSeq Stranded Total RNA Gold to remove rRNA, and libraries were prepared using Illumina TruSeq kit.