title: GSM6172301: Whole Genome CRISPR Screen; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was isolated using the NucleoSpin Blood XL-Maxi kit (Clontech Cat. # 740950) with RNase A (Clontech Cat. # 740505) treatment. DNA concentration was measured with the Quant-iT™ PicoGreen™ dsDNA Assay Kit (ThermoFisher Scientific, #P11496). Whole genome CRISPR sgRNA pBrie library designed by Broad Genetic Perturbation Platform (GPP). OTHER: CRISPR sequencing.