title: Biological repetition 1 of p50 injected with AcMNPV
design: Total RNAs were extracted using TRIzol reagent (Invitrogen, USA) and RNA quality was evolveded by Agilent 2100 Bioanalyzer. Genomic DNA was removed from a 3 g total RNA aliquot by using 10 g DNase I (Takara, Japan) for 30 min at 37 C. RNA was purified from the digest using Dynabeads Oligo (dT) 25 (Life Tech, USA). Finally, the cDNA library of each sample was constructed using 100 ng purified mRNA with aNEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA).