title: GSM5657686: SW-780-24hr-Untreated-Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Following incubation, media was aspirated fully and 0.6mL RLT Buffer with β-mercaptoethanol (from Qiagen RNeasy Plus kit) was added to wells and plates were frozen at –20°C. Plates were thawed on ice, wells were scraped, and contents transferred to QIAshredder columns. RNA was isolated according to the RNeasy Plus kit instructions (Qiagen), RNA was eluted, quantified, aliquoted for RNAseq submission and/or qPCR, then frozen at –80°C. RNA-seq libraries were prepared Illumina® TruSeq RNA Library Prep Kit v2 and were sequenced using Illumina® NovaSeq to obtain 150-bp paired-end reads