title: Illumina HiSeq 2000 sequencing; RNA-seq of human umbilical vein endothelial cells (HUVECs) stimulated with recombinant Vascular Endothelial Growth Factor A (VEGF-A) against untreated control
design: RNA-seq of human umbilical vein endothelial cells (HUVECs) stimulated with recombinant Vascular Endothelial Growth Factor A (VEGF-A) against untreated control
library_construction_protocol: HUVECs were grown 24 hours in complete medium except for VEGF-A and then induced with VEGF-A or vehicle for six hours Total RNA from HUVECs was extracted with Trizol. Nuclei acid library for polyadenilated transcripts were constructed following standard protocol and they are not strand specific.