title: GSM5472249: SS2_20_121:G1; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was added to 500 uL of 0.05% Trypsin/ 0.02% EDTA (Sigma, 59417-C) and incubated at 37° C for 10 min. The tissue was triturated slowly with a P-200 pipette until complete dissociation. Trypsin was deactivated by adding 500 uL of PBS containing 10% FBS.  The cells were pelleted at 500 g +4C for 5 min. The cells were washed 2x with PBS + 10% FBS followed by centrifugation. The non-single cell clusters of cells were removed by filtering through 40 μm-pore size cell strainer. Fluorescent cells were sorted in single cells mode into 384 well plates prefilled with lysis buffer according to previously published protocol (21) using a BD FACSAria III Cell Sorter. One full 384-well plate was sorted and sequenced per organ and per treatment. RNA libraries were prepared according to Smart-seq2 protocol.