title: Whole-exome sequencing of patient samples: 070b_1_S9_WES
design: "Genomic DNA (gDNA) was isolated from peripheral blood leukocytes (PBL) or bone marrow mononuclear cells (MNC) using QIAGEN Puregene Blood Core Kit or DNeasy Blood & Tissue Kits (QIAGEN Sciences, Germantown, MD, USA).  Integrated DNA Technologies (IDT)  xGen Lockdown Probes were used to the following genes: ASXL1, BCOR, CBL, CEBPA, CEBPB, DNMT3A, ETV6, EZH2, FLT3, GATA2, IDH1, IDH2, IKZF1, JAK2, KIT, KRAS, MECOM, NPM1, NRAS, PTPN11, RUNX1, SETBP1, SF3B1, SRSF2, STAG2, TET2, TP53, U2AF1, WT1, ZRSR2.  Libraries were prepared via themanufacturer's instructions and sequenced on an Illumina HiSeq4000. "