title: GSM5918081: PA-1 DGCR8 knockout replicate 2; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA from either wildtype, DGCR8 heterozygous or DGCR8 knockout PA-1 cells was extracted from a 60% confluent T75 cell flask (Gibco) with the miRNeasy Mini Kit (Qiagen), following the protocol for Purification of Total RNA, Including Small RNAs, from Animal Cells was followed, including the additional on-column DNAse digestion. After extraction, ≥ 200 ng of total RNA was used for library sequencing with DNBseq SE50 by BGI Genomics.