title: GSM6065742: C. crescentus empty vector - replicate 2; Caulobacter vibrioides CB15; RNA-Seq
library_construction_protocol: Cell pellets were resuspended in 1ml TRIzol, then stored at -80°C. Samples were heated at 65°C for 10 min. 0.2 ml chloroform was mixed into samples and incubated for 5 min. Samples pelleted at 17,000xg for 15 min at 4°C. Aqueous layer transferred to fresh tube. 450 µl isopropanol was added and stored at -80°C overnight. Thawed samples were pelleted at 4°C for 30 min, then supernatant was removed. Pellets were washed in 1 ml 70% ethanol, dried, and resuspended in 100 µl RNase-free H2O. Samples were cleaned up with RNeasy Mini Kit (QIAGEN). RNA was sent to Microbial Genome Sequencing Center for library preparation and sequencing. Libraries were prepared with Illumina Stranded mRNA Prep and RiboZero Plus rRNA depletion.