title: GSM5115671: Huh7.5 fentanyl-treated rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: The NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA) was utilized for polyA RNA purification with a total of 300 ng total RNA as input. A PrepX mRNA Library kit (WaferGen) combined with Apollo 324 NGS automated library prep system was used for library preparation.