title: GSM5917923: 9_VBTM3_Day3Cas; Plasmodium vivax; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol® (Invitrogen), DNAse treated and purified using the RNeasy MinElute Cleanup Kit (Qiagen). Prior Trizol, samples with iCas9-GFP 3T3-J2 cells were treated with a ﻿chemical inducer of dimerization (B/B Homodimerizer) to partially deplete the fibroblast cells via apoptosis. RNA was quality controlled using an AATI Fragment Analyzer and 100ng was prepared following the SureSelectXT RNA Direct protocol version A0. Illumina libraries were quantitated using the Fragment Analyzer and by qPCR and sequenced as a single end 40nt read using a HiSeq2000.