title: Illumina HiSeq 2500 sequencing; RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
design: RNA-seq comparing WT to Dax1, Nr5a2 and Dax1/Nr5a2 knock-out clones in 2iLIF and SL differentiation conditions
library_construction_protocol: total RNA collected Cells grown in 2iLIF on gelatin coated plates G6C18 mouse embryonic stem cells, transfected with gRNAs AGAGCCGACCGCATGCGAGG and AAAAGTTCCAGTATCAGGTG, mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)