title: RNA-Seq of Rattus norvegicus: adult male liver
design: Single-end reads of 50 bp length were produced with a minimum of 1M reads per sample. Quality control of raw reads was performed with FastQC 0.11.7. Adapters were filtered with ea-utils fastq-mcf v1.05. Splice-aware alignment was performed with HiSAT2 against the Rattus norvegicus reference genome (genome build=Rnor6.0). Reads mapping to multiple loci in the reference genome were discarded. Resulting BAM files were handled with Samtools v1.5. Quantification of reads per gene was performed with HT-seq Count v2.7.14.