title: Paired-end Illumina sequencing of Clostridium botulinum strain 426-HONEY isolated from contaminated honey in 1977
design: Strain grown anaerobically for 24h at 35C in 25 mL TPGY broth. Whole culture was pelleted and resuspended in 0.5 mL of GPB with 0.5 mL of 2x Zymo DNA/RNA Shield. DNA extracted using Zymo Quick-HMW kit and RNAseA treatment, and prepared with NexteraXT library prep; paired-end (2x300bp) sequencing using MiSeq and v3 chemistry.