title: GSM5658229: RNA-Seq-shCTL-MCF7; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Mini Kit following the manufacturer's protocol. DNase I in column digestion was included to ensure the RNA quality. RNA library preparation was performed by using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (E7420L). Paired-end sequencing was performed with Illumina HiSeq platform.