title: GSM5011992: Tspo mutant MA-10 cells rep2; Mus musculus; RNA-Seq
library_construction_protocol: The cells used for total RNA extraction were cultured in 6-well plates to 80% confluence. Total RNA samples from Wt and Tspo mutant cells were extracted using TRIzol  reagent (Invitrogen; Thermo Fisher Scientific, Waltham, MA, USA) The quantified RNA samples were used for NEBNext® (New  England Biolabs, Ipswich, MA, USA) rRNA-depleted (Human/Mouse/Rat) stranded  library preparation To remove rRNA from the total RNAs