library_construction_protocol: FACS-sorted cells were loaded onto a 10x Chromium microfluidics system according to the manufacturer's guidelines. As the guidelines, scRNA-seq 5′ gene expression (GEX) libraries were generated using the 10X Genomics Chromium Single Cell 5′ Kit version3.1 and the 10x Chromium Controller (10x Genomics) according to the 10x Single Cell 5′ version3.1 protocol guidelines (CG000204). RNA libraries were prepared for sequencing using standard Illumina protocols