library_construction_protocol: Total RNA was extracted from the aforementioned rice roots using TRIzol reagent (ThermoFisher Scientific, Cat # 15596026). 30 µg total RNA was used for DNase I treatment followed by mRNA enrichment using oligo d(T) beads. The enriched mRNA was subsequently used for RNA-seq library preparation following the  standard protocol for preparing Illumina RNA-seq libraries.