library_construction_protocol: Samples were flash frozen on dry ice, and RNA was harvested using the GeneJET RNA purification kit (Cat#K0732) was used to isolate total RNA for the construction of sequencing libraries cDNA libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Kapa Biosystems, Wilmington, MA) according to the manufacturer's protocol