library_construction_protocol: Cells were directly sorted into Trizol using Fluorescence-activated cell sorting. Total RNA was extracted with the RNeasy Micro Kit and integrity was confirmed with an Agilent 2100 Bioanalyzer. Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit. Libraries were barcoded for multiplexing. Before sequencing, library quality and concentration were measured using Qubit Fluorometric Quantitation