library_construction_protocol: Total human brain RNA was derived from post mortem cortex and provided by Agilent (Agilent Technologies, cat: 540143). For the preparation of mouse brain RNA, a male mouse (strain 129S2/SV) was sacrificed, and the entire brain was harvested and grained in Trizol (Thermo Fisher Scientific). Total RNA was obtained according to the manufacture's protocol. ONT cDNA sequencing libraries were prepared according to the protocol, SQK-PCS108, version PCS_9035_v108_revF_26Jun2017