library_construction_protocol: RNA was isolated using TRIzol and total RNA was amplified using SMART-seq V4 Low Input RNA Kit Amplified cDNA was used to prepare Illumina HiSeq libraries with the Kapa DNA library preparation chemistry. Samples were barcoded and run on Hiseq 2500 1T, in a 50bp/50bp paired-end run using the TruSeq SBS Kit v3