library_construction_protocol: Colonic mucosal surfaces were cut and isolated from freshly dissected full-thickness colonic tissue, then placed into Trizol. RNA was precipitated with isopropanol and cleaned with multiple washes of 75% ethanol prior to resuspension in water. Libraries were constructed using the Illumina Stranded mRNA library preparation kit to generate single-end reads 1x75bp on NextSeq High Output Runs.