library_construction_protocol: IMR90 cells were harvested, and the RNA was extracted using RNeasy mini Kit (Qiagen, cat. no. 74106) and digested with DNase I (Qiagen, cat. no. 79254) The libraries were prepared using a KAPA RNA HyperPrep kit (Roche, cat. no: 07962312001) and sequenced in a 75-bp paired-end run at the Wistar Genomics Facility using an Illumina NextSeq 500 system