library_construction_protocol: Freshly isolated DCs are immediately processed for extraction. An aliquot of each cell suspension was stained with trypan blue.  Concentration and viability were determined using the BioRad TC20 automated cell counter. Final library concentration was determined using the Qubit High Sensitivity dsDNA Assay .  Libraries were normalized to a common concentration prior to being pooled and sequenced on the Illumina HiSeq-4000. Single cell libraries were prepared using the 10X Genomics Chromium V2' Gene Expression Solution