library_construction_protocol: Fed and unfed tadpoles at 10 and 16 dpf were anesthetized, and the GI tubes were isolated using a thin razor and forceps. A total of 20–26 intestinal tubes from each stage were collected and homogenized in TRIzol LS reagent (#10296010; Thermo Fisher Scientific). RNA was purified using RNA Clean & Concentrator-5 (R1014; Zymo Research, Irvine, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols