library_construction_protocol: Spleen singlet cell suspension were made, stained with anti-mouse CD4, CD25, CD127 antibodies, then CD4+CD25highCD127low cells were purified by Aria Cell Sorter directly into TRIzol, and RNA was harvested using miRNeasy Mini kit. Total RNA libraries were prepared by RNA Library(T01), and Illumina sequencing PE150, 40M clean reads were generated for bioinformatic analysis.