library_construction_protocol: (1000 cells) were lysed in guanidine thiocyanate buffer (TCL buffer, Qiagen) supplemented with 1% b-mercaptoethanol. RNA was isolated by solid-phase reversible immobilization bead cleanup using RNAClean XP beads (Agentcourt), reverse-transcribed and amplified Uniquely barcoded libraries were prepared using Nextera XT kit (Illumina) following manufacturer's instructions