library_construction_protocol: Endosperm from maize 80-100 maize kernels from 6 to 8 DAP. Endosperm were incubated in a enzymatic solution to digest cell walls and produce protoplasts. Protoplasts were filtered using a cell strainer and used as input for the 10x Chromium Single Cell 3' V3 pipeline. Libraries were prepared using Chromium Single Cell 3' Reagent Kit V3.