library_construction_protocol: The isolated intestine tissue was digested into  cell suspension with dispase. Cells were loaded on a GemCode Single Cell Instrument(10x Genomics, USA) to generate single cell Gel bead in Emulsion (GEMs). ScRNA-seq libraries were prepared using the GemCode Single-Cell 3' Gel Bead, Chip and Library Kits (10x Genomics, USA) as per the manufacturer's protocol. Libraries were sequenced on a Nova6000.