library_construction_protocol: The tissue was collected first thing in the morning (9–10:00 am) following birth and was snap-frozen on dry ice and stored at −80 °C until. RNA was extracted using the Direct-zol RNA Miniprep kit. Libraries were prepared with the KAPA mRNA HyperPrep kit (Roche Sequencing solutions) from total RNA of RQN > 8.5 as assessed by the Fragment Analyser system (Agilent Technologies)