library_construction_protocol: 5000 from 3 mice each of BAL AM, exAM, texAM or Host AM were lyzed in lysis buffer. After centrifugation, nuclei were resuspend in reaction buffer containing Tn5 transposase. Tagmented DNA was purified using SPRI beads. Library amplification using nextera kit has been done as described in Lara-Astiaso et al., 2014.