library_construction_protocol: Whole zebrafish larvae at 3 dpf were genotyped before collection in RNA-later. Total RNA was isolated from whole zebrafish larvae at 3 dpf from atp6v1e1bcmg78zebrafish and atp6v1e1bhi577aTg zebrafish together with their respective controls using TRIzol® Reagent followed by a purification with the RNeasy mini kit (Qiagen) according to manufacturer's instructions. RNA integrity was checked by 2100 Bioanalyzer (Agilent). 10 zebrafish larvae of each genotype were pooled in 1 sample to obtain sufficient heterogeneity amongst samples Sequencing library was prepared according to the manufacturer's instructions of the TruSeq® Stranded mRNA Library Prep (Illumina, San Diego, California, United States) supplemented with TruSeq® RNA Single Indexes Set A (Illumina).