library_construction_protocol: Single cells were captured in the 10X Genomics Chromium Single Cell 3' Solution, and RNA-seq libraries were generated following the manufacturer's instruction (10X Genomics) and subjected to high-throughput sequencing on an Illumina Novaseq PE150 platform. single cells were captured using the 10X Genomics Chromium Single Cell 3' Solution according to the manufacturers protocol. (10XGenomics, Pleasanton) high-throughput sequencing were on Illumina Novaseq PE150 platform.