library_construction_protocol: Single-cell suspension was prepared following 10X Genomics Single cell protocols: Cell preparation guide (CG00053, Rev C). Single Cell Labelling with the BD™ Single-Cell Multiplexing Kits was performed following protocol Doc ID: 210970 Rev. 2.0 23-21340-00 Droplet-based single-cell partitioning and single-cell RNA-Seq libraries were generated using the Chromium Single-Cell 3′ Reagent v2 Kit (10X Genomics, Pleasanton, CA) as per the manufacturer's protocol based on the 10X GemCode proprietary technology