library_construction_protocol: Cells were collected from culture using standard procedures. Mouse epiblast stem cells were cultured in conditioned medium (CF-1 feeders) with 5 ng/ml FGF2 and 10 ng/ml Activin A. Cells were stably transfected with a piggybac-TRE-Lima1-IRES-Venus plasmid and expression was induced with doxycycline for 48h. Total RNA was isolated using Macherey-Nagel RNA isolation kit. 1ug of this was used for library preparation and sequencing. Enrichment of mRNA was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module and cDNA library was constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit.