library_construction_protocol: Yolksacs were placed into Tail Digestion Buffer with Proteinase K at 55ºC overnight with no shaking. The following day the samples were incubated at 95ºC for 10 minutes to inactivate Proteinase K. The DNA was precipitated with ethanol and sodium acetate, washed two times with 70% ethanol and the pellet was dissolved in 200ul 10mM Tris pH7.5. The gDNA was then processed through the nCATS protocol followed by the Oxford Nanopore minION library construction kit (SQK-LSK109).