library_construction_protocol: The first-passage NSCs were harvested, each group contained samples of 2 mice.RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Illumina, USA) was used  for the construction of sequencing libraries.The libraries were size-selected by agarose gel electrophoresis, PCR-amplified, and sequenced by HiSeq x Ten. RNA libraries were prepared for sequencing using standard Illumina protocols