library_construction_protocol: RNA was isolated using Qiagen RNAEasy Micro kit from 5,000 -10,000 cells and cDNA generated and amplified using NuGEN Ovation RNA-Seq System v2 kit. Libraries were constructed from cDNA with the TruSEQ RNA Library Preparation Kit v2 Illumina and sequenced to 30–40 × 106 1 × 75-bp reads per sample on a HiSEQ 2500