library_construction_protocol: Total RNA of male and female adult Anopheles sinensis was extracted separately using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. The Poly (A) RNA was purified from 200 μg total RNA using one round Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) purification. RNA libraries were prepared for sequencing using standard Illumina protocols