library_construction_protocol: Peyers patches were collected, CD4+ T cells bead purified (Miltenyi Biotec) and CD45.2+CD45.1-CD4+CD8-TCRβ+ OTII T cells were sorted on FACS ARIA scRNA-seq libraries were made using a Chromium Single Cell 3′Library & Gel Bead Kit v2 (P/N 120737, 10x Genomics) and sequenced