library_construction_protocol: RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The extraction weas perfrom by UCLA TCGB core RNA libraries were prepared for sequencing using standard Illumina protocols. The library construction was perfromed by UCLA TCGB core. single cell T cell receptor profiling