library_construction_protocol: Total RNA was extracted using the Norgen Total RNA isolation kit (Cat #48400) and quantified using a NanoDrop Spectrophotometer ND-1000 Library prep was performed using the NEBNext Ultra Directional RNA Library Prep Kit along with the NEBNext Poly(A) mRNA Magnetic Isolation Module. Libraries were subject to further BioAnalyzer QC and quantified by qPCR.