library_construction_protocol: Muscles removed, flash frozen in liquid nitrogen. Homogenized with TRIzol and Qiagen ceramic beads to extract RNA (Qiagen, Tissue Lyser II). Library prepped using Poly-A tail enrichment of mRNA using NEBNext® Ultra™ II Directional RNA Library kit. Sequenced on Illumina NextSeq550, 150bp paired end reads.