library_construction_protocol: IELs were extracted from mice colons and sorted leukocytes were then loaded onto to the 10X ChromiumTM Single Cell 3' Chip to be encapsulated forming Gel Beads in Emulsion (GEM) and cDNA was generated as per 10X genomics protocol. Single cell RNA sequencing libraries were prepared according to 10X Genomics protocol and using the ChromiumTM Single Cell 3' Library and Gel Bead Kit (v2)