library_construction_protocol: Nuclei was extracted by lyse cells in lysis buffer containing NP40 and protease inhibitor. DNA library was generated by biotin fill-in followed blunt end ligation. Then removal of biotin from un-ligated ends, sonication, end repair, biotin pull-down, Illumina adaptor ligation and paired end PCR. Hi-C library generated Hi-C 2.0 protocol. Belaghzal et at.2017. 