title: The chloroplast genome sequence of Acer mandshuricumtree
design: After extracting genomic DNA by CTAB method, the DNA was fragmented by mechanical interruption (ultrasonic), and then the fragment was purified, repaired at the end, A was added to the 3 'end, and the sequencing connector was connected. Then the fragment size was selected by agarose gel electrophoresis, and the sequencing library was amplified by PCR.