title: GSM6131808: hnRNP R WT, rep 4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel). RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for all samples was >9.6. cDNA libraries were prepared from 300 ng of total RNA with TruSeq mRNA Stranded Library Prep Kit from Illumina according to manufacturer's instructions (1/2 volume). Libraries were quantified by QubitTM Flex Fluorometer (ThermoFisher) and quality was checked using Fragment Analyzer system 5200 with DNF-474 HS NGS Kit (Agilent).