title: GSM5945010: Ins10 S96; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was performed using TRIzol reagent according to the manufacturer's instructions. DNA digestion was perfomed using the RNEasy mini kit from Qiagen according to manufacturers' instruction The library preparation was performed according to the manufacturer's protocol using the 'VAHTS™ Stranded mRNA-Seq Library Prep Kit for Illumina®'. Briefly, 300 ng total RNA were used for mRNA capturing, fragmentation, the synthesis of cDNA, adapter ligation and library amplification.