title: 16s rRNA amplicon sequencing of mus  musculus: feces
design: For  DNA extraction, Quick-DNA Fecal/Soil Microbe kit column-based, bead beating  step included. In triplicate, the V4 region of the 16S rRNA gene was  amplified with barcoded PCR primers F515 and R806 modified to contain an  adapter region for sequencing on the Illumina MiSeq platform. Amplicons were  verified by gel electrophoresis, combined, purified, and sent to the UC Davis  Genome Center for library preparation and high throughput 250-bp paired-end  sequencing using the Illumina MiSeq platform.