title: GSM6133241: ATAC-seq_S1_25k; Mus musculus; ATAC-seq
library_construction_protocol: MinElute (before PCR, Qiagen) and AMPure XP (after PCR, Beckman Coulter) were used for DNA purification. Cells were lysed with CSK buffer (10 mM PIPES pH 6.8, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 0.1% Triton X-100). Nuclei were treated with Tn5 Transposase (Illumina). The total of 8 PCR cycles were performed to recover and amplify the DNA fragments at open chromatin.