title: GSM4785480: WGBS_col_rep2_for_T2_T3; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: For T2 and T3 WGBS, DNA from inflorescences of adult plants was extracted following a CTAB-based method.  For T4 and T5 WGBS, DNA from inflorescences of adult plants was extracted using the DNeasy Plant Mini Kit (QIAGENE). For T2 and T3 WGBS, 100 ng of DNA was sheared to 200 bp with a Covaris S2 (Covaris) and used for library preparation using the Epitect Bisulfite Conversion kit (QIAGEN) and the Ovation Ultralow Methyl-seq kit (NuGEN) following the manufacturer's instructions. For T4 and T5 WGBS, 250 ng of DNA was sheared to 200 bp with a Covaris S2 (Covaris) and used for library preparation using the Epitect Bisulfite Conversion kit (QIAGEN) and the Kapa DNA hyper kit (Kapa Biosystems) with Illumina TruSeq DNA adapters following the manufacturer's instructions.