title: GSM5238633: 27HC_2_5; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. ITruSeq Stranded mRNA Sample Prep Kit was used for the construction of sequencing libraries The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand and Read 2 aligns to the SENSE strand. * The libraries were quantitated by qPCR and sequenced on two lanes for 101 cycles from each end of the fragments on a HiSeq 4000 using a HiSeq 4000 sequencing kit version 1.