title: the DSS group_rep5
design: To explore the influence of anthocyanins on the immunopathology associated with CTLA-4 blockade, we establish ICI-associated autoimmune mouse model through After a one-week adaptation, 8 weeks old female C57BL/6 mice were randomized by body weight into four experimental groups. Mice in the control group were given normal drinking water, and mice in the other three groups were given 3% DSS in drinking water for 6 d, and the date of starting DSS drinking water was recorded as the first day. For the DSS-CTLA-4 group, mice were intraperitoneally injected with 100 g anti-CTLA-4 mAb on day 0, 2 and 5, respectively. Accordingly, mice in the DSS group were intraperitoneally injected with the isotype control antibody. On the basis of DSS-CTLA-4 group, the BAE-PECTIN group was given 25 mg bilberry anthocyanin extracts/kg body weight and 100 mg low molecular citrus pectin/kg body weight by intragastric administration from day 1. For the DSS-CTLA-4 group mice were given isovolumetric sterile water as the BAE-PECTIN group. All mice were sacrificed by cervical dislocation under anesthesia with Avertin at day 12, then distal colon tissues were collected for transcriptome analysis. The RNA-seq sequencing library was built using the Illumina TruseqTM RNA sample prep Kit