title: ITS metabarcoding of Microdochium isolate strain 2
design: The ITS2 region of the fungal rRNA locus was amplified using ITS3_KYO2 (5-GAT GAA GAA CGY AGY RAA-3) and ITS4 (5-TCC TCC GCT TAT TGA TAT GC-3) primers (Toju et al., 2012). The libraries containing fungal ITS2 region were sequenced on the MiSeq platform using MiSeq Reagent Kit v2 (500-cycles) (Illumina).