title: input DNA for H3K9me3 ChIP-seq from T98G GBM cell line treated with siRNA targeting HJURP
design: Chromatin-immunoprecipitation (ChIP) assay was performed using SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, 9003). In brief, 4 x 10^6 T98G cells silenced for HJURP (siHJURP) and control cells (siControl) were used for immunoprecipitation. Chromatin was immunoprecipitated using 5ug of anti-H3K9me3 (ABCAM, ab8898) with overnight incubation at 4C with rotation. Immunoprecipitated DNA samples were quantified using a Qubit dsDNA HS Assay Kit. DNA quality and appropriate distribution of fragment sizes were verified using Agilent Technologies 2100 Bioanalyzer. ChIP DNA libraries were performed using IlluminaTruSeq ChIP Sample Preparation Kit, and subsequent DNA sequencing was obtained using NextSeq 500/550 High Output Kit v2 (300 cycles) in NovaSeq 6000 equipment (Illumina).