title: AsCas12a-Ultra RNP targeting ARR3 in HEK293 cells using ARR3-18-S guide RNA
design: Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.