title: CTRL002 SAMMY-seq S2
design: "For DNA fractionation, we took an aliquot corresponding to 30% of volume of S2, S3, S4 fractions and added TE buffer to reach a final volume of 300 uL. After incubation with RNAse A (Roche) (90 minutes at 37o) and Proteinase K (Sigma) (150 minutes at 55o), DNA was extracted from beads by standard phenol/chloroform extraction, precipitated and resuspended in 25 ul milliQ H2O. After Nanodrop (260/280 = 1,7-1,9; 260/230 >= 2) and Qubit HS DNA quantification, we added H2O to a final volume of 105 uL. Then samples were transferred to 96 well plates and sonicated 4 times with Bioruptor sonicator (10 minutes 30 seconds ON - 30 seconds OFF, High Power). The DNA profiles were finally checked by capillary electrophoresis (Agilent 2100 Bioanalyzer). Finally, DNA libraries were prepared by using NuGEN Ovation Ultralow Library Prep System kit and then sequenced using an Illumina HiSeq 2500 instrument according to manufacturer's instructions (Illumina)."