title: GSM5366570: DGM-13478 (mRNA-seq); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from oral biopsies of nonsmokers and electroic cigarette vapers was extracted with TRIzol with subsequent RNA clean-up using the RNeasy MinElute RNA pu-rification kit to remove residual DNA The TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) was utilized as per protocol to generate cDNA that was then purified with QIA-quick PCR purification kit (Qiagen, Valencia, CA) Samples were loaded onto an Illumina flowcell for paired-end 101 x 2 sequencing reactions using the Illumina HiSeq 2000, using a single flow cell with 4 samples multiplexed per lane of flow cell. HiSeq Control Software (Illumina) was used to perform image analysis, base calling and sequence analysis on HiSeq generated sequence images.