title: RNA-Seq of 2-day-old adult female abdomen
design: Samples were fast-frozen in liquid nitrogen and preserved at -70C. With the exception of 1st instar larvae and adult heads, which were mechanically homogenized in TRIzol using Teflon homogenizers, the rest of sample types were weighed after pestle homogenization in a ceramic mortar using liquid nitrogen, adjusting for sample quantity prior to be stored in TRIzol. Total RNA was subsequently extracted using Direct-zol RNA MiniPrep extraction kit (Zymo Research) according to manufacturer recommendations, except for all pupa samples and adult abdomens which were first extracted with TRIzol also following manufacturer recommendations and then purified with Direct-zol columns. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina).