title: GSM5945672: C3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Thirty seedlings (entire organisms) from each replicate were harvested and flash frozen using liquid nitrogen, then pulvarized using a mortar and a pestle. RNA extraction was performed using the standard Trizol method. RNA was purified using RNeasy RNA-Extraction Kit (Qiagen) following the manufacturer's protocols. 1 µg of RNA was used to construct libraries using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina). RNA libraries were constructed with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) following the protocol provided by Illumina. RNA-seq libraries were purified, amplified and paired-end sequenced (100-nt length) on two flow cells of Illumina NovaSeq-6000 Sequencer using  S1 100PE v1.5 chemistry at the Genome Sequencing Facility of the University of Kansas Medical Center.