title: GSM6133606: WT100_-6hAPF_Wing_30mCnR_aIgG_Sup_Rep1; Drosophila melanogaster; OTHER
library_construction_protocol: A minimum of 50 SG and 90 wings or legs were individually dissected in 1xPBS The Takara Thruplex DNAseq kit with unique dual-indexes was used following manufacturer's instructions until the amplification step. For amplification, a combined, 10s annealing / extension step at 68C was used.