title: 16S metagenome of mus musculus: caecum microbiome
design: Caecum sample was treated with Proteinase K at 56C for 2 hours prior to DNA extraction with a commercial DNA extraction kit (bead beating/column binding). NanoDrop values were determined and 16S PCR was performed for the extracted DNA present in the caecum microbiome. 16S metagenomic sequence library was generated with Illumina MiSeq platform.