title: RNA-Seq of Rattus norvegicus: pineal gland
design: Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit following manufacturers recommendations and index codes were added to attribute sequences to each sample. Total mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEBNext First Strand Synthesis Reaction Buffer(5X).