title: Illumina MiSeq Amplicon Sequencing of general fungi in German soils
design: Sampling took place in May 2011, 2014 and 2017 in all EPs of the Germany Biodiversity Exploratories. Along a transect, 14 soil cores of the upper 10 cm of soil were taken, compiled, sieved and stored at -20C. Amplification of ITS2 with fITS7 (GTGARTCATCGAATCTTTG) and ITS4 (TCCTCCGCTTATTGATATGC).