title: GSM6132403: LNCaP, CSS castration + enzalutamide3; Homo sapiens; RNA-Seq
library_construction_protocol: The RNA was isolated by RNeasy Mini Kit from Qiagen. The integrity of total RNA was examined by the Agilent 4200 TapeStation System. Libraries for RNA-Seq were constructed with Nugen Universal plus mRNA-Seq Kit to generate strand-specific RNA-seq libraries. The workflow consists of poly(A) RNA selection, RNA fragmentation and double-stranded cDNA generation using a mixture of random and oligo(dT) priming, followed by end repair to generate blunt ends, adaptor ligation, strand selection, and PCR amplification to produce the final libraries. Amplified libraries were quantified by Qubit dsDNA HS (High Sensitivity) Assay Kit, and quality-checked by the Agilent 4200 TapeStation System. Different index adaptors were used for multiplexing samples in one sequencing lane. Sequencing was performed with HiSeq3000 sequencer to produce 50 base-pair single-end reads (1 x 50 bp).