title: GSM6131736: AMT+Ab BS-seq rep2; Oryza sativa; Bisulfite-Seq
library_construction_protocol: RNA was isolated from leaves of transgenic rice under different treatment groups using RNeasy Plant mini kit (Qiagen) or Trizol for RNA-seq libraries. Genomic DNA was extracted from leaves by using a DNeasy Plant Mini Kit (Qiagen). RNA-seq libraries were prepared following standard Illumina protocols according to manufacturer's instructions. The fragmented DNA was then used for library preparation using Illumina's library preparation kits. Premethylated adapter ligated fragments were bisulfite converted using the EZ DNA methylation kit (Zymo Research). The BS-seq libraries were then subjected to PCR amplification prior to sequencing on the Illumina HiSeq 2500 (Illumina) sequencers.