title: metagenome of PIIBS
design: DNA is purified from human stool samples using MO BIO Laboratories PowerSoil DNA Isolation Kit, MP Biomedicals FastPrep-24 5G Homogenizer and an automated high throughput nucleic acid isolation system (Perkin Elmer Chemagic MSM I) based on proprietary M-PVA bead technology. The concentration measured using Qubit dsDNA HS Kit (PN Q32854 Thermo Fisher Scientific Inc., Waltham, MA).