title: 7661 distal colon
design: RNA was extracted (RNeasy Plus, Qiagen) and quantity was verified with the Quant-iT RiboGreen RNA Assay Kit (Fisher Scientific) and samples were normalized to a concentration of 25 ng/L. RNA quality (RNA integrity number > 9 for all samples) was confirmed by Agilent TapeStation capillary electrophoresis. RNA sequencing (RNA-seq) was performed in the UCLA Neuroscience Genomics Core Laboratory using Lexogen QuantSeq 3 FWD cDNA library synthesis and multiplex DNA sequencing on an Illumina HiSeq 4000 instrument with single-strand 65nt sequence reads following standard manufacturer protocols