title: CanadaRed mat Meta T
design: Total RNA was extracted followed by Agencourt RNA Clean XP kit (Beckman Coulter) clean up the RNA.  Ribosomal RNA was removed using Ribo-Zero Magnetic kits (Illumina). An Agilent 2100 Bioanalyzer was used to evaluate the quality of rRNA removal. rRNA subtracted RNA was used for library construction using a Scriptseq v2 RNA-seq Library Preparation Kit (Illumina). An Agilent 2100 Bioanalyzer was used to verify libraries.