title: GSM6133303: H9 cells, RNA-seq, rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from all samples in parallel using the RNeasy Kit (Qiagen) following the manufacturer's instructions and quantified before running on an Agilent RNA Pico 6000 chip using an Agilent 2100 Bioanalyzer. RNA with a RIN score above 7 was used to generate RNA libraries Libraries were prepared using the NEBNext UltraII RNA library preparation kit and NEBNext rRNA depletion kit (New England Biolabs). cDNA libraries were generated from 750 ng of RNA, amplified for 8 PCR cycles, and barcoded with NEBNext Multiplex Oligos for Illumina (New England Biolabs).