title: GSM4785646: Normal 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with Isogen (Nippon gene, Japan) and RNeasy spin column kit (Qiagen, USA). 1 µg of RNA samples with RNA integrity number (RIN) greater than 9 were used for library prepairation. RNA-Seq libraries were prepared using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego CA) according to the manufacturer's instructions and subsequently validated for an average size of about 305-310 bp using a 2100 Bioanalyzer and the Agilent DNA1000 kit for the construction of sequencing libraries.