title: RNA-Seq of Leptobryum pyriforme: green gametophytes
design: mRNA was enriched from the total RNA by oligo (dT) magnetic beads. The purified mRNA was then broken into short fragments, which was used as template to synthesize the first- and second-strand cDNA. After reverse transcription, cDNA libraries were generated from appropriate size of DNA fragments using NEBNext Ultra RNA Library Prep Kit (NEB, USA).