title: GSM5734179: 265_Batch4_50_6832_S108_L008; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from the endocervical cytobrush samples collected at the crossover visit using RNeasy Micro kit (Qiagen, CA) with DNase digest and QIAcube automation stations. The RNA was quantified, and the quality was assessed by Bioanalyzer analysis (Agilent Technologies, Santa Clara, CA) Libraries were prepared using the Illumina (Illumina Inc. San Diego, CA, USA) TruSeq RNA Exome kit, as per manufacturer's instructions, with 20 ng of total RNA as input. The libraries were quantified using Qubit dsDNA HS before normalization and pooling. The amplified libraries were assessed for their quality and sizing information by capillary electrophoresis on the Agilent 4200 TapeStation. The libraries were normalized, pooled and sequenced on the Illumina HiSeq3000 system employing a single-end 101 cycles run at average read depths of 23 million reads/sample.  