title: GSM6133851: HEK293T UL1KOKO CN 1 [CN_1_HEKUL1KO]; Homo sapiens; RNA-Seq
library_construction_protocol: Nucleolar and cytoplasmic-nuclear fractionation was carried out according to the protocol. After fractionation, the following fractions are obtained: NO – nucleolar, CN – cytoplasmic-nuclear. RNA was isolated using the Quick-RNA MiniPrep Kit (Zymo Research, R1055) according to the manufacturer's protocol. CORALL Total RNA-Seq library preparation Kit (Lexogen, Vienna, Austria) was used to prepare libraries, without ribosomal RNA depletion, following the manufacturer's instructions.