title: GSM5740017: hFOB_WT5_HA; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed in 1% formaldehyde for 10 minutes prior to quenching with excess glycine. Cells were resuspended on ice in a cell lysis buffer (10 mM Tris-HCL, 10 mM Nacl, 0.5 % NP-40, all reagents were from Sigma-Aldrich) for 15 minutes, centrifuged, and resuspended in nuclear lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1 % SDS, all from Sigma-Aldrich) for 10 minutes.  Samples were diluted with 2 volumes of dilution buffer (16.7 mM Tris-HCL, 167 mM NaCl, 1.2 mM EDTA, 0.01 % SDS, all from Sigma-Aldrich) and sonicated on a Bioruptor Pico (Diagenode, Belgium) for 10 cycles of 30 seconds on/30 seconds off. Sonication efficiency was checked by running a sample of de-crosslinked material on a 2 % agarose gel. Libraries were prepared using the NEBNext Ultra 2 DNA Library Preparation Kit (New England Biolabs, MA, USA)