title: Y. ruckeri long read sequencing
design: Bacteria grew in the final volume of  2L Luria Broth until OD 600 0.3 was reached. DNA was extracted with Qiagen Genomic Tip 100/G (Cat No./ID: 10243) and Genomic DNA Buffer Set (Qiagen, Cat No./ID: 19060), by following suggested instructions. DNA concentration and quality were tested with Nanopore, Qubit and TapeStation. Prior sequencing samples were prepared with Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion 1-12 (EXP-NBD104) and finally sequenced by Oxford Nanopore MinION.