title: eDNA-cantabric-seawater
design: Environmental DNA was employed to detect species present in water samples, using Next Generation Sequencing (NGS) as explained in Garcia-Vazquez et al. (2021). In summary, DNA was extracted from membranes and ethanol pellets using PowerWater DNA Isolation Kit (Qiagen). A fragment of the cytochrome oxidase I gene (COI thereafter) was amplified on eDNA samples using the primers mlCOIintF43 (Leray et al., 2013) and jgHCO219844 (Geller et al., 2013) modified with a PGM sequencing adaptor, sample barcodes and a GAT spacer. PCR was conducted in a Veriti Thermal Cycler (Applied Biosystems, Foster City, CA) employing the mix and conditions described in detail in Garcia-Vazquez et al. (2021).