title: GSM6133945: 3rd instar whole larvae, CrolKO, RNA-seq, rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For the extraction of RNA, the L3 larvae of wild-type (oregon) and mutant were collected in a PBS buffer (40 larvae per sample), in three biological repeats. Total RNA was extracted with the TRI reagent (Ambion). PolyA comprising RNA fraction was isolated and prepared for sequencing with the NEBNext Ultra™ II Directional RNA Library Prep Kit. New generation sequencing was performed by Evrogen (evrogen.ru) with the Illumina NovaSeq6000 sequencer.