title: SoctosporusAzygotic_19hours_RNA_seq
design: Total RNA was extracted from S. octosporus cells undergoing azygotic meiosis. The integrity and concentration of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). A total amount of 2 g RNA was used as input material for RNA-seq library preparation. Barcoded sequencing library was generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, #E7530L) following the manufacturer's recommendations. The library was sequenced on the Illumina Hiseq Xten platform and 150 bp paired-end reads were generated.