title: GSM5366301: HT1376_CPI-0209_H3K27me3; Homo sapiens; ChIP-Seq
library_construction_protocol: 1x107 HT1376 cells treated with or without CPI-0209 (250 nM) for 4 and 8 days were fixed by adding formaldehyde to final concentration of 1%. Fixation was done for 10 min at room temperature. Crosslinking was stopped by adding glycine to a final concentration of 125 mM. The fixed cells were snap-frozen and shipped by Active Motif for ChIP-seq processing (Carlsbad, CA). Briefly, ChIP reactions were performed with 30 ug of extracted chromatin with antibodies to EZH2 (Active Motif #39901), H3K27Ac (Active Motif #39133) and H3K27me3 (Active Motif #39155). 