title: GSM5237148: Mutant mice for NEHI model; Mus musculus; RNA-Seq
library_construction_protocol: Cellular suspensions were loaded on a 10X Genomics Chromium Controller (10x Genomics, Pleasanton, CA) to generate single cell GEMs. And ~1200 cells were loaded per well. cDNA extraction and amilification were excecuted using Chromium Single Cell 3' Reagent Kits (v3 Chemistry, PN-1000075, 10X Genomics) folloing User Guide provided by 10X Genomics. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3' Gel Bead and Library Kit v3 (PN-1000075, 10x Genomics) following User Guide provided by 10X Genomics. scRNA-seq library for 10X Genomics sequencing