title: GSM5945640: Gonadal_HFD_HET_7; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of GnW and liver was extracted using the miRNeasy mini kit (Qiagen) Total RNA was quality checked (RIN >7) via the Agilent Bioanalyser 2100 system, using the Agilent RNA 6000 Nano Kit.  1ug of RNA was used to construct barcoded sequencing libraries with the TruSeq Stranded mRNA HT Sample Prep Kit (Illumina) following the supplier's instruction. All the libraries were validated using the Agilent Bioanalyser DNA 12000 and then multiplexed and sequenced on two lanes of Illumina HiSeq 4000 at SE50 at CR-UK Cambridge Institute Genomics Core Facility.