title: GSM5737197: WT E14 MN (Sample 11); Mus musculus; OTHER
library_construction_protocol: CUT&RUN was performed as described previously by Skene and Henikoff, 2017 and Meers et al., 2019. 100K to 200K cells were sorted for double-positive (Hoxa5-P2A-mCherry and Hoxa7-P2A-EGFP) populations. Wild-type MNs were treated similarly and collected through FACS. The cells were re-counted after sorting and the published protocol by Skene et al., 2018 detailed in “https://www.protocols.io” by Janssens, D. and Henikoff, S. was followed. Libraries were prepared according to manufacturer's instructions (Illumina) as published in the protocol by Skene et al., 2018, detailed in “https://www.protocols.io” by Janssens, D. and Henikoff, S.