title: sc_RNA seq of iPSC-derived sensorimotor organoids day 16: Sample A2-R1
design: Single-cell RNA-seq was performed by microfluidic inDrop encapsulation, barcoding, and library preparation, as previously described17,107 by the Single Cell Core at Harvard Medical School, Boston, MA. In brief, three independent biological replicates of the 11a iPSC line were differentiated for 16 days. Cells were then dissociated with Trypsin-EDTA and encapsulated into droplets using a microfluidic device. Each of the biological triplicates resulted in three 3,000 cell libraries, totaling 9,000 cells per biological sample. Next generation sequencing of the cDNA libraries was performed in collaboration with the Center for Cancer Computational Biology at the Dana-Farber Cancer Institute using an Illumina NextSeq 500 next generation sequencer.