title: 16srDNA of soil
design: Paired-end reads were assigned to samples based on their unique bar codes and truncated by cutting off the bar code and primer sequence. To obtain high-quality clean tags, QIIME (http://qiime.org/index.html) was used to perform quality filtering on the raw tags under specific filtering conditions. The effective tags were obtained after removal of chimera sequences. Sequences with 97% similarity were assigned to the same operational taxonomic unit abundances