title: GSM6133409: anterior segments, mutant, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Anterior segments were dissected in PBS by cutting along the limbus with scissors. After removing the lens, the remaining anterior segment (cornea, iris, ciliary body and ocular drainage structures) was collected. Samples were stabilized in RNAlater (ThermoFisher Scientific, USA) and stored at −80 °C until use. Each sample were pooled from 4 eyes from 2 mice. Total RNA was extracted using the RNeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols