title: 16S rRNA gene v3-v4-V5 amplicon of activated sludge bioreactor
design: Bacterial 16S rRNA amplicon sequencing was done in two steps. Primer set 341f/785r targeted the V3-V4 variable regions of the 16S rRNA gene. The libraries were sequenced on an Illumina MiSeq platform (v.3) with 20% PhiX spike-in and at a read-length of 300 bp paired-end.