title: Genome sequence analysis of Tribolium castaneum-adapted E. coli Nissle 1917 (8x48h midgut persistence)
design: Total genomic DNA was isolated using the MagAttract HMW DNA kit (Qiagen, Hilden, Germany). To prepare 500 bp paired-end libraries of all isolates we used the Nextera XT DNA Library Preparation kit (Illumina, San Diego, CA, USA).