title: GSM6133835: ChIPSeq_P7_THY1+_uSPG_H3K27me3_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Tissues/THY1+ uSPGs were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Branson sonifier (0.9 s on/0.1 s off x 7 cycles, average size 300-700 bp). ChIP-seq libraries were prepared using the NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina. 10 ng of DNA was used as starting material for input and ip samples. Libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were amplified using 7 cycles on the thermocycler. Libraries were validated using the Agilent High Sensitivity DNA Kit