title: Microbiome of the Amazon River mouth and Atlantic ocean.
design: DNA libraries were prepared using the Nextera XTSample Preparation Kit (Illumina). Library size distributionwas determined using the Agilent 2100 Bioanalyzerand High Sensitivity DNA Kit (Agilent) and quantifiedusing the Applied Biosystems 7500 Real-Time PCR Systemand KAPA Library Quantification Kit (Kapa Biosystems). The sample was sequenced using MiSeq System (Illumina), paired-end 2  250 bp