title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of allelic series of mouse Huntingtin knock-in cells
design: RNA-seq of allelic series of mouse Huntingtin knock-in cells
library_construction_protocol: We collected cellular pellets from day 8 and day 28 of neural differentiation. RNA was isolated using TRIzol reagent according to manufacture's instruction (LIfe Technologies), quantified with Nanodrop. DNA contaminants were removed by DNA-free kit (Ambion). Libraries were constructed with the stranded Poly-A method.