title: GSM5059333: H3K4me3_NCI-H322M_shKdm5b_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked with formaldehyde and subsequently sonicated. Sonicated cell extracts were used for ChIP assays. ChIP-enriched DNA was end-repaired using the End-It DNA End-Repair kit (Epicentre), followed by addition of a single A nucleotide, and ligation of adapters. PCR was performed using Phusion High Fidelity PCR master mix