title: GSM6133390: vWAT; cort injection; day 7; rep. 4; Mus musculus; RNA-Seq
library_construction_protocol: Fat depots (BAT and vWAT) were removed, flash frozen in N2, and RNA was extracted using the RNeasy Lipid Tissue Mini Kit (Qiagen, Hilden, Germany). mRNA samples were concentrated to ≤ 5 µl by MinElute column (QIAGEN, Cat. 74204). For generation of RNA-seq libraries, polyadenylated mRNA was isolated from 300 ng of total RNA by incubation with oligo-DT attached magnetic beads and followed by strand-specific library preparation using the TruSeq Stranded mRNA Library Preparation kit (Ilumina, Cat. 20020595).  Samples were pooled and sequenced on the Illumina NextSeq 500/550 High Output platform (Illumina, FC-404-2002) up to 18 samples per lane with 1% PhiX spike as a control.