title: Partial 18S rRNA amplified from rhizosphere soil of Rhynchosia venulosa
design: PCR amplicons were purified using the Agent Court AMPure XP beads (Beckman Coulter, Brea, CA, United States). The libraries were amplified with a limited-cycle PCR program (12 cycles) to add the index 1 (i7) and index 2 (i5) adapters, containing sequences required for cluster generation of the Illumina flow cell. The quality and sizes of the resulting DNA fragments were evaluated on a 1.5% (w/v) agarose gel. The libraries were quantified with a fluorometric method (Qubit, Life Technologies) and normalized to 4 nM using a standard dilution method. Subsequently, the amplicon libraries were pooled, and paired-end sequencing was done on an Illumina MiSeq platform using a MiSeq Reagent Kit V3 600 cycles (Illumina, San Diego, CA, United States).