title: 33 Day Rectum1
design: The V6V8 region of the 16S rRNA gene was amplified using PCR primers 799F (acCMGGATTAGATACCCKG) and bac1193R (CRTCCMCACCTTCCTC). Amplification was performed using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94 C for 3 min, followed by 28 cycles of 94 C for 30 s, 53 C for 40 s and 72 C for 1 min, with a final elongation step at 72 C for 5 min. PCR products were confirmed using a 2% agarose gel. PCR products were then used to prepare DNA libraries following Illumina MiSeq DNA library preparation protocol.