title: Complete Genome of the Probiotic Streptococcus salivarius SALI-10
design: High-quality DNA (1.0 g) isolated from S. salivarius SALI-10 strain was sheared and the continuous long reads (CLR) DNA library was prepared following the Pacific Biosciences 20-kb template preparation using the BluePippin size selection system protocol. DNA was fragmented using the Covaris g-TUBE device and then end-repaired to prepare SMRTbell DNA template libraries (with a fragment size of 15kb to 50kb) selected using a BluePippin system. SMRT 8M sequencing was performed on PacBio Sequel II system.SMRT Link v10.1.0.119588 was used for fully automated demultiplexing, assembly, circularization, and polishing of both chromosomes and plasmids to produce gold standard references.Achieve high-quality consensus accuracies >99.99%. Output data in standard file formats (BAM and FASTA/Q) for seamless integration with downstream analysis tools.