title: 16S rRNA gene amplicon sequencing of wheat associated soil metagenome
design: For Illumina 16S rRNA gene amplicon sequencing, the amplicon library construction was done as described previously in Yergeau et al. (2015) based on a dual-indexing strategy that followed the 16S Metagenomic Sequencing Library preparation Illumina guide (Part #15 044 223 Rev. B). The V4 hypervariable region was amplified using the universal primers 520F (5 - AGCAGCCGCGGTAAT- 3) and 799R2 (5 - CAGGGTATC TAATCCTGTT- 3) (Edwards et al. 2007), that exclude plant plastids 16S rRNA genes. PCR products were purified, pooled and submitted for 2  250 bp Illumina MiSeq sequencing at the CESGQ.