title: GSM5238716: i35_SF_P368_F2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using a Direct-zol MicroPrep kit with DNase treatment (Zymo Research, Irvine, CA, USA) following the manufacturer's instructions. RNA was extracted using a Direct-zol MicroPrep kit with DNase treatment (Zymo Research, Irvine, CA, USA) following the manufacturer's instructions. RNA concentration was measured using a NanoDrop spectrophotometer. RNA quality of eight randomly chosen samples were assessed using High Sensitivity RNA ScreenTapes (Agilent) on an Agilent 2200 TapeStation. Library preparation was done using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with poly-A selection (Illumina, San Diego, CA, USA) following the manufacturer's instructions. Every library was quantified for DNA content with High Sensitivity DNA ScreenTapes (Agilent). Libraries from 24 samples with unique identifiers were pooled and run on an Illumina NextSeq 500 using the 75 cycles NextSeq High Output kit (Illumina). bulk RNA-Seq