title: GSM5672422: Rspo3 mutant enteroid RNA-seq sample #1; Mus musculus; RNA-Seq
library_construction_protocol: Libraries were prepared according to the NEBNext Ultra II Directional RNA kit protocol following Ribosomal Depletion Cells were lysed using the lysis buffer described in Chu, T. et al. Nature Genetics (2018). Chromatin was pelleted and supernatant was collected and stored in Trizol LS. RNA was isolated using manufacturer's protocol.