title: RT_SC3
design: RNA samples with RIN values greater than 8 were processed for downstream library preparation and sequencing. Briefly, 60 ng total RNA per sample extracted from Tetracapsuloides bryosalmonae sorted cells, were used for library preparation with the Poly(A) RNA Selection Kit V1.5 and the CORALL mRNA-Seq Library Prep Kit (Lexogen, Vienna, Austria) according to the manufacturers protocol. Library quality control was done with the D1000 ScreenTape assay (Agilent, Santa Clara, CA, USA) on the 4200 TapeStation. Sequencing of libraries (paired-end 150-bp reads) were performed on llumina NovaSeq 6000 platform.