title: Parus major personality RRBS F2C: blood
design: The Control and bisulfite-treated libraries were prepared with the Hyper Library Construction kit from Kapa Biosystems, according to the "Reduced Representation Bisulfite Sequencing For Methylation Analysis" protocol from Illumina. Samples were digested with MspI, which generates CCGG overhangs. The digested DNAs were size selected for fragments 20-200bp in length. The libraries were quantitated by qPCR and each pool was sequenced on one lane for 101 cycles from one end of the fragments on a HiSeq2500 using a HiSeq SBS sequencing kit version 4. Fastq files were generated and demultiplexed with with the bcl2fastq v2.17.1.14 Conversion Software (Illumina). The lanes were spiked with PhiX to provide nucleotide diversity. The PhiX reads have been removed from the dataset. Adaptors have been trimmed from the reads. Sequence of adaptors used to make the libraries (used blue bold portion for adaptor trimming). Adaptor sequence in read1:AGATCGGAAGAGCACACGTCTGAACTCCAGTCACNNNNNNATCTCGTATGCCGTCTTCTGCTTG (NNNNNN= 6 nt index)