title: GSM6131998: Iso CD4+ T cells GEX seq 2; Mus musculus; RNA-Seq
library_construction_protocol: For single cell RNA sequencing, Lungs from TC-1 tumor bearing Pdcd1fl/fl × Foxp3eGFP-Cre-ERT2(+/-) mice were digested with type IV collagenase and DNaseI mixture. Digested lung samples were stained with CD4 antibody and the LIVE/DEAD fixable dead cell stain kit for sorting of live CD4+ T cells. Tumor infiltrating CD4+ T cells were sorted from isotype control or anti-PD-1 antibody treated Pdcd1fl/fl × Foxp3eGFP-Cre-ERT2(+/-) mice. Single-cell sequencing libraries were prepared by using Chromium 10X Genomics platform with a VDJ Enrichment Kit and 5' Library & Gel Bead Kit according to the manufacturer's instructions. Libraries were constructed using Chromium™ Single Cell 5' v1 Reagent Kits following the manufacturer's protocol