title: HiSeq X Ten paired end sequencing; RNA-seq data of rs2280381-containing region knock out clone in U-937 cell line
design: RNA-seq data of rs2280381-containing region knock out clone in U-937 cell line
library_construction_protocol: we deleted a 120-bp region at rs2280381 in the U-937 cell line using CRISPR/Cas9 with two sgRNAs targeted around rs2280381 loci. Plasmids expressing Cas9 and sgRNAs were nucleofected into cells, and cell clones were screened to obtain bi-allelic 120-bp deletion clones. Clones exposed to CRISPR/Cas9 complexes with wildtype genotype were selected as controls.  Total RNA was extracted using TRIzol reagent (15596018, Thermo Fisher)  1 g total RNA was purified to remove rRNA and was used to generate the cDNA libraries using Illumina NEBNext Ultra Directional RNA Library Prep Kit