title: RAD-seq of Speckled Dace (Rhinichthys osculus)
design: Genomic DNA was extracted with a magnetic beadbased protocol (Ali et al. 2016) quantified using Quant-iT PicoGreen dsDNA Reagent (Thermo Fisher Scientific) with an FLx800 Fluorescence Reader. RADseq libraries were created with the SbfI enzyme and Best Rad (Ali et al. 2016). Libraries were sequenced with a paired-end 100bp layout on an Illumina HiSeq 2500.