title: Amplification of V1-V3 region of microbial 16S rRNA from D. simulans: female ovary
design: Primers covering the V1-V3 regions of bacterial 16S rRNA gene, 27F (5- AGAGTTTGATCCTGGCTCAG) and 534R (5-ATTACCGCGGCTGCTGG), were used to amplify and sequence on an Illumina MiSeq with V3 chemistry. Target totals of 50,000 reads per sample were attained from 25-35cycles of PCR.