title: GSM5740158: 350Veh; Mus musculus; RNA-Seq
library_construction_protocol: RNA from the sorted cells was isolated using the RNeasy Plus Micro kit (QIAGEN). Quality control was performed by bioanalyzer (Agilent), and RNA samples with an RNA integrity number (RIN) > 8 were processed for library preparation using SMARTer Ultra Low Input reagent (Takara) and Nextera XT DNA (Illumina) library preparation kits Libraries were sequenced with paired-end reads of 126bp on a HiSeq2500 sequencer (Illumina) to reach 50 million read pairs per sample. For each group, IFN or vehicle, and genotype, data are derived from three distinct mice, with separate processing from cell sorting to sequencing.