title: GSM6133431: RNA-seq, vel3, 24HAI, R1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA of endosperm and seeds were extracted using Qiagen RNeasy Plant Mini Kit Libraries of RNA-seq and H3K27me3 ChIP-seq were construed by Novogene (China) and BGI (China) respectively.  For libraries of VEL3 ChIP-seq, Tn5 tagmentation was performed on 1ng ChIP-ed DNA or input DNA for 5 min at 55°C, with 0.1ul enzyme and 2.5ul buffer in 5ul reaction (Illumina, 20034197). PCR amplification was conducted using the following programs: 72°C  5min, 98°C 30s, N cycles of 98°C 10s, 63°C 30s and 72°C 1min. cycle number (N) was determined by the qpcr of each partially-amplified library. 0.8X Agencourt AMPure XP beads (Beckman Coulter, A63880) was used for the library size selection. Libraries were sequenced at Novogene (Beijing, China) using a Novaseq system, 150-bp paired-end sequences with a minimum of 30 million reads acquired per sample.