title: Cold-Treatment-12hours
design: Methods: After the five five week-old seedlings were separated into control and treatment groups, it they  were treated  with low temperatures stress  at a constant temperature and light in an incubator (MODEL: MLR-352H-PC) of the Cell Genetics Laboratory of Maize Research Institute of Sichuan Agricultural University (Chengdu, China) utilizing a complete block design . The low temperature and control groups were constantly  maintained at 0 and 25, C, respectively,  at an illumination of 3,000 Lux. Subsequently, we obtained leaves after 12 h, 36 h and 60 h oflow temperature treatment for RNA-seq and related indices.