title: GSM6133857: EYE treated with 75% oxygen Postnatal day13(P13), VLDLR deletion; Mus; RNA-Seq
library_construction_protocol: new birth mice (postnatal day 7,P7 treated with 75% oxygen for five days P12,then placed into air feed, Ocular tissues were then taken at P12(immediately after put back into the air) and P13.Total RNAs were extracted from the tissues and send to company for high-throughput circular RNA sequencing Total RNA was removed the rRNA,and linear RNA ,the resultant was then fragmented and used to sequencing The modified cDNAs fragments with length ranging from 150 bp to 200 bp were used for library construction.  Following cluster generation, the paired-end sequencing was conducted on an Illumina Hiseq 4000 platform.