title: GSM5945218: radiation-8w rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from the rectal tissues of mice in both irradiated and control group at 4 hours, 1 day, 3 days, 2 weeks and 8 weeks post-irradiation was extracted using Trizol reagent (Invitrogen, Carlsbad, CA). The mRNA was isolated by NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB, E7490). The cDNA library was constructed following the manufacturer's instructions of NEBNext Ultra RNA Library Prep Kit for Illumina（NEB, E7530）and NEBNext Multiplex Oligos for Illumina（NEB, E7500).