title: Illumina sequencing of Ferrovum dominated stalactite biofilm from acid mine drainage
design: Total genomic DNA was isolated from stalactite biofilm using DNeasy PowerLyzer PowerSoil kit (Quiagen, Germany). The Illumina sequencing libraries were prepared using TruSeq Nano DNA high-throughput kit. The fragmentation of DNA was performed on Bioruptor Plus sonication device. The libraries were sequenced using the Illumina HiSeq 2500 RR 2x250 bp technology.