title: Illumina MiSeq of mud snail Tritia obsoleta bacteria: ingesta, gut, feces
design: DNA extracted using Qiagen DNeasy PowerSoil Kit with some modifications to increase yield; amplicon libraries prepared following a modified version of the the Illumina 16S metagenomic library prep protocol using primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 (Klindworth et al. 2013), amplifying V3 and V4 regions of the SSU rRNA gene; sequencing performed on an Illumina MiSeq (2 x 300 bp) twice.