title: WGS of United States invasive Streptococcus pyogenes isolates
design: Shearing protocol - 500 bp protocol using sonication (Covaris) Library preparation kit - SparQ DNA Library (QuantaBio)   KAPA qPCR quantification (KAPA Biosystems) Sequencing instrument - Miseq Sequencing kit - Illumina MiSeq Sequencing kit v2 (500 cycles)