title: GSM6133283: H9 cells, TOP2A CUT&RUN, rep 4; Homo sapiens; OTHER
library_construction_protocol: TOP2A and TOP2B CUT&RUNs were performed using the CUT&RUN Assay Kit (Cell Signaling) and following the manufacturer's instructions starting with ~ 100,000 cells and performing the antibody incubation overnight at 4C. CUT&RUN libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB) with minor modifications to avoid denaturation and loss of small DNA fragments.