title: NextSeq 500 sequencing; smallRNA-seq of Solanum Lycopersicum and Solanum Pennellii parents and crosses up to the F4 generation.
design: smallRNA-seq of Solanum Lycopersicum and Solanum Pennellii parents and crosses up to the F4 generation.
library_construction_protocol: 1-4cm young leaves were collected in less than 2h for all plants at the same time of the day using liquid N2 greenhouse grown plants, Approx 24C day, 18 night. 16/8h day/night Trizol isolation. Trizol reagent (Invitrogen) according to the manufacturer's instructions. Total RNA isolation was performed with the Direct-zol RNA Miniprep (Zymo Research)  NEBNext® Small RNA Library Prep Set for Illumina® (Multiplex Compatible)