title: GSM5733118: large artery atherosclerotic stroke 6; Homo sapiens; RNA-Seq
library_construction_protocol: Plasma  were collected, extracted exosomes, and RNA was obtained using miRNeasy Serum/Plasma Advanced kit. RNA quantification and qualification. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA), and RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols. The sequencing libraries were generated by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.