title: Illumina HiSeq 4000 sequencing; RNA-seq of mouse primary somatosensory cortex (S1) in a neuropathic pain model after electroacupuncture stimulus
design: RNA-seq of mouse primary somatosensory cortex (S1) in a neuropathic pain model after electroacupuncture stimulus
library_construction_protocol: Mouse somatosensory cortex was dissected after anesthetization The cleaved RNA fragments were reverse-transcribed to create the final cDNA library in accordance with the protocol for the mRNASeq sample preparation kit (Illumina, San Diego, USA), the average insert size for the paired-end libraries was 300 bp (Â±50 bp).