title: NextSeq 500 paired end sequencing; Lymphoma-induced regression of high endothelial venules impairs homeostatic lymphocyte trafficking
design: Lymphoma-induced regression of high endothelial venules impairs homeostatic lymphocyte trafficking
library_construction_protocol: The cell suspensions containing sorted BECs (7AAD-Ter119-CD45-PDPN-CD31+) were processed for single-cell RNA sequencing (scRNAseq) using the Chromium Single Cell 3' library and Gel Bead Kit v2 (10X Genomics) according to the manufacturers guidelines. Single-cell RNA sequencing The cell suspensions containing sorted BECs (7AAD-Ter119-CD45-PDPN-CD31+) were processed for single-cell RNA sequencing (scRNAseq) using the Chromium Single Cell 3' library and Gel Bead Kit v2 (10X Genomics) according to the manufacturers guidelines. The cell suspensions containing sorted BECs (7AAD-Ter119-CD45-PDPN-CD31+) were processed for single-cell RNA sequencing (scRNAseq) using the Chromium Single Cell 3' library and Gel Bead Kit v2 (10X Genomics) according to the manufacturers guidelines.