title: GSM5366291: 7373_3; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were collected at indicated interim timepoints on treatment or at study endpoint for analysis at 1 hr post-dosing for pharmacokinetic (PK) and pharmacodynamic studies (PD), unless indicated otherwise. Tumors were split into two pieces (for PK and PD), snap-frozen in liquid nitrogen, then transferred to -80°C before analysis. Total RNA was extracted with Trizol, in accordance with the manufacturer's protocol. Sequencing libraries were constructed using the Illumina TruSeq RNA Sample Preparation Kit (v2). The resulting libraries were sequenced on an Illumina HiSeq 4000, with 9 samples multiplexed per lane.  2x150 base pair paired-end reads using the Illumina TruSeq strand specific protocol, for an expected 40 million reads per sample.