title: HTR-8/Svneo -  PCBP2 siRNAs - sample 2
design: Total RNA was isolated from HTR-8/SVneo cell lines transiently transfected with siRNAs directed against PCBP2 and non-targeting control siRNA using the Qiagen RNeasy Mini Kit. The KAPA RNA Hyperprep kit including ribodepletion (Roche) was used for the construction of the sequencing libraries, following manufacturer's instructions. RNA-seq libraries were sequenced (paired end) using the Illumina HiSeq 4000 (2 x 150 bp) with 8 samples per lane. The 8 samples libraries were multiplexed across two sequencing lanes.