title: NextSeq 500 sequencing; RNA-seq experiment of sv2a-/-, sv2a+/- and sv2a+/+ zebrafish larvae
design: RNA-seq experiment of sv2a-/-, sv2a+/- and sv2a+/+ zebrafish larvae
library_construction_protocol: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas).  TruSeq RNA-Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.