title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of blood samples from patients diagnosed with visceral leishmaniasis and asymptomatic individuals (Brazil)
design: RNA-seq of blood samples from patients diagnosed with visceral leishmaniasis and asymptomatic individuals (Brazil)
library_construction_protocol: Peripheral blood samples from healthy donors, asymptomatic individuals and patients were collected using BD Vacutainer and PAXgene Blood RNA tubes. Total RNA samples were purified with the PAXgene Blood RNA Kit and GLOBINclear-Human kit. Libraries were prepared using a TruSeq Stranded RNA Sample Preparation kit (Illumina) with Poly(A)+ selection.