title: GSM5737983: 30w; Equus caballus; Bisulfite-Seq
library_construction_protocol: DNA was isolated from tissue using a Sherlock AX (A&A Biotechnology) kit To quantify DNA methylation levels across the genome RRBS methodology was used following the Ovation RRBS Methyl-Seq System (NuGEN) protocol. 12 DNA libraries were pooled in one pool and sequenced on the NexSeq 500 platform (Illumina) using a 75-bp single-end read and High-Output Flow Cell. The sequencing was performed along with a balanced control library obtained from the viral DNA (PhiX, Illumina).