title: rumen metagenome of Bos taurus: MASG_003
design: Genomic DNA was extracted from 250 l of each thawed and homogenized ruminal content sample, using the DNeasy Power Soil commercial kit (QIAGEN, Valencia, CA, USA). Oxford Nanopore Technologies (ONT) SQK-LSK109 Ligation Sequencing kit was used for multiplexed sequencing in MinION automatic sequencer. The 1D Native barcoding ONT kit (EXP-NBD104 or EXP-NBD114) was used for multiplexing the samples, pooling barcoded DNA from 12 samples for each run. Pooling was done using a 1.5 ml DNA LoBind tube to perform adapter ligation and sequenced using a R9.4.1 flow cell.