title: GSM4835420: Male Fru Plus R1; Drosophila melanogaster; ATAC-seq
library_construction_protocol: Nuclear isolation and Tn5 transposition were performed as in Buenrostro J et al., 2015 with modifications made for small numbers of cells as described in Monahan K et al. 2017. Transposed DNA was amplified with barcoded primers in NEBNext High Fidelity 2X PCR Master Mix (NEB) and purified with Ampure XP beads (Beckman Coulter) at a ratio of 1.6uL beads per 1uL library. Purified library was eluted in 30uL of 10mM Tris-HCl pH8, 0.1mM EDTA. The quality of prepared libraries was verified with a Bioanalyzer 2100 using a high sensitivity DNA kit (Agilent). Libraries were quantified using KAPA qPCR assay (KAPA Biosystems), and multiplexed and sequenced on a NextSeq Illumina machine with 75 base pair, paired-end reads.