title: GSM6133909: SK-N-BE(2)C cells, Control rep1 (ATAC-seq); Homo sapiens; ATAC-seq
library_construction_protocol: Cells were trypsinized using a cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). Following lysis, nuclei were centrifuged at 500 × g for 10 min. To avoid losing cells during the experiment preparation, we used a fixed-angle centrifuge. After nuclei preparation, a Tn5 transposition reaction was performed using a Nextera Kit (Illumina, San Diego, CA, USA), according to the manufacturer's protocol. Directly following transposition, DNA was purified using a MinElute Kit (QIAGEN, Hilden, Germany). Llibrary amplification was performed using a NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, MA, USA) with SYBR Green I dye (Invitrogen, Waltham, MA, USA) and Nextera PCR primers Ad1 and Ad2. The amplified libraries were purified using a PCR purification kit (QIAGEN) and quality control was performed using the 2200 Tapestation (Agilent, Santa Clara, CA, USA).