title: Mouse gut microbiota in response to flaxseed and its oil and lignan components
design: DNA from cecum content was extracted using a commercial DNA Isolation Kit. The DNA library preparation was performed according to Illumina 16S sequencing protocol using primers targeting the 16S rRNA hypervariable region V3-V4. Paired-end 2??300 bp sequencing was performed on Illumina MiSeq platform using MiSeq 600-cycle v3 kit