title: GSM6176026: Dissolved oxygen limited iron 21% rep1; Escherichia coli; RNA-Seq
library_construction_protocol: Total RNA was isolated from the frozen cells in Trizol samples using the hot phenol method. The ribosomal RNA was depleted from the purified total RNA by using the RiboMinus Transcriptome Isolation Kit (Invitrogen, Carlsbad, CA, USA). The RNA-seq libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, Ipswich, MA, USA).