title: RNA-seq of Bothrops jararaca: Lung mRNA from adult female
design: Extracted total RNA from all tissues, ground in Polytron PT3100 homogenizer and total RNA was isolated with TRIZOL reagent (Invitrogen). PolyA+ RNAs were obtained by magnetic bead purification (DYNAbeads, LifeTechnologies). The mRNA concentration was estimated by Quant-iT RiboGreen kit (Invitrogen). The quality of total RNAs and mRNAs was evaluated by electrophoresis on picoRNA chip using the Bioanalyzer system (Agilent Technologies)