title: GSM5013437: Muscle stem cells - GFP; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and DNA complexes were isolated with antibody. Library was prepared according to NEBNext Ultra DNA Library Prep Kit (E7370) by the steps of end-polishing, dA-addition and the DNA fragments with A tail were ligated to sequencing adaptors. After 100bp-500bp size selection and PCR amplification, the DNA libraries were quantified and sequenced on HiSeq2500 platform using single-end 50bp sequencing based on manufacturer's instructions.