title: GSM5945417: Cochlea macrophage old adult 12 [CO_Mp_Ol_12]; Mus musculus; RNA-Seq
library_construction_protocol: Dissections were performed to isolate whole cochlea or cochlea substructures (auditory nerve or lateral wall). A tissue isolate from one mouse was treated as an independent sample. Samples were dissociated and sorted for GFP-positive cells (macrophages) and GFP-negative cells (nonmacrophages) by FACS using a procedure adapted from Panganiban et at., 2022. Total RNA was purified from each sample using the RNeasy Micro kit (Qiagen). Quality of each total RNA preparation was assessed by 4200 TapeStation, with low-quality samples excluded from further analysis. RNA libraries were prepared using the SMARTer Ultra and Nextera XT library prep. Sequencing was performed on an Illumina HiSeq 2500 using Rapid Run mode, SINGLE end read, 1X50 Cycles. Sequencing was done a second time for a subset of the samples.