title: 16S amplicon sequence of mycorrhiza CMCC ROC8
design: All qualified DNA was used to construct libraries. For 16S PCR products, the jagged ends of DNA fragments were converted into blunt ends by using T4 DNA polymerase, Klenow Fragment and T4 Polynucleotide Kinase. Then an 'A' base was added to each 3' end to facilitate addition of adapters. After all that, the fragments too short were removed by Ampure beads. The primer used was a fusion primer with dual index and adapters for PCR. Only the qualified library was used for sequencing followed by bioinformatics analysis.