title: metatranscriptomic of periphytic biofilm
design: Total RNA was extracted using RNA Purification Kit (Thermo Fisher Scientific, MA, USA) according to the manufacturers instructions.  RNA degradation and contamination were monitored on 1% agarose gels.  RNA quantity was measured using Qubit 2.0 (Thermo Fisher Scientific, MA, USA) and Nanodrop One (Thermo Fisher Scientific, MA, USA) together.  RNA integrity was accurately detected using the Agilent 2106 system (Agilent Technologies, Waldbron, Germany).