title: GSM5366694: RNA_K562 cells-AAVS1-pg1-rep2; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted using RNeasy Mini Kit (QIAGEN 79254), and three replicates were arranged. 2 μg of total RNA was further used for the construction of sequencing libraries. The RNA-seq libraries were prepared using the NEBNext PolyA mRNA Magnetic Isolation Module (NEB E7490S), NEBNext RNA First Strand Synthesis Module (NEB E7525S), NEBNext mRNA Second Strand Synthesis Module (NEB E6111S) and NEBNext Ultra DNA Library Prep Kit for Illumina (NEB E7370L).