title: GSM5014034: GRO_TTX_KCl_10M_REP5; Mus musculus; OTHER
library_construction_protocol: RNA was extracted using acid phenol for GRO-seq. Protein was UV-crosslinked with RNA, cross-linked samples incubated with antibodies, and RNA was purified for eCLIP-seq. Protein was crosslinked with DNA, cross-linked samples incubated with antibodies, and genomic DNA was purified for ChIP-seq. GRO-seq libraries was prepared as previously described (C. G. Danko, Mol Cell 50, 212-222, 2013). eCLIP-seq libraries was prepared as previously described (E. L. Van Nostrand, Nat Methods 13(6):508-14, 2016). ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set (NEB).