title: M9-R1-2
design: Bacteria were grown in M9 media and collected at five stages of growth to evaluate the span of gene expression. RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo, USA) following the manufacturers bead beating and DNase I treatment protocol. RNA was prepared for sequencing with the NEBNext rRNA depletion kit (E7850, New England Biolabs, USA), followed by the Stranded Total RNA Prep (Illumina, USA) following the manufacturers protocol