title: 16s rRNA sequencing of microbiota of WT mice: feces
design: DNA extraction and amplification Genomic DNA were extracted from fecal material using the QIAamp DNA Stool Mini Kit. PCR amplification were performed using 515F/806R Illumina primers targeting the V4 region of the 16S rRNA gene. Amplicons were sequenced using Illumina NovaSeq 6000 platform.