title: GSM5920256: bladder tissue (7 days after spinal cord injury) rep1 [S1-1]; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted from bladder tissues using TRIzol  and quantified using a spectrophotometer . Ribosomal RNA was depleted from total RNA using a RiboMinus kit. RNA-Seq was conducted using a HiSeq 2500 Sequencing System with a single-end 50 bp.RNA-seq libraries were constructed using the Illumina TruSeq protocol. RNA libraries were prepared for sequencing using standard Illumina protocols