title: RNA-seq of Trypanosoma brucei: RBP42 KD day3
design: RNA was extracted using TRIzol (ThermoFisher) after three days following RBP42 knockdown. Poly(A)+ -containing RNA, captured by two rounds of oligo-d(T)n-bead selection of 10 g total RNA, and libraries were made following Illumina small RNA library preparation