title: GSM5366390: PT12-MT Cycle 1 Day 1; Homo sapiens; RNA-Seq
library_construction_protocol: Whole blood cells were collected using PAXgene blood RNA tubes. Proccessing and sequencing of patient RNA samples were performed by ALMAC. The RNA extractions were performed using PAXgene Blood RNA Kit following manufacturer's instructions. Library preparation proceeded with rRNA and hemoglobin depletion using KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin. Paired-end 75 base pair sequencing was performed on an Illumina platform. Prior to sequencing, RNA integrity was assessed by 2100 Bioanalyser (Agilent) and concentration was assayed by Nanodrop. 