title: RNA-Seq of musculus: young surgery hippocampi 3
design: The sequencing RNA libraries were constructed by performing a 3'-end linker, a 5'-end linker, reverse transcription, amplification, cDNA library size selection, and purification steps on isolated RNA. The purified libraries were quantified by a Qubit 2.0 Fluorometer (Life Technologies, USA) and validated by an Agilent 2100 Bioanalyzer (Agilent Technologies, USA) to confirm the insert size and calculate the mole concentration. Clusters were generated by cBot with the library and then sequenced with a Paired-End module on the Illumina HiSeq 2500 (Illumina, San Diego, CA, USA).