title: GSM6132148: toyocamycin_10hr_2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from experiments in biological triplicates was isolated using RNeasy Mini Plus Kit (QIAGEN) following the manufacturer's instructions. RNA amount and purity was measured using Qubit 3.0, and (3) Agilent2100 was used to check for RNA Integrating Number (RIN). Samples with RIN values of >8.5 were sent to Novogene Library construction. Briefly, the polyA enrichment library preparation was performed to construct 250-300 bp insert cDNA library. Sequencing was performed using paired end reads (150 bp, average 40 million reads per sample) on the HiSeq2500 platform.