title: GSM5365665: HUVEC in the absence of neutralizing antibody against PDGFB_3; Homo sapiens; RNA-Seq
library_construction_protocol: Extraction of total RNA was performed using TRIzol reagent (Thermo Fisher, MA, USA) according to the manufacturer's protocols. Briefly, RNA samples were quantified using fluorimetric RiboGreen assay and quality was assessed using an Agilent 2100 Bioanalyzer. Total RNA (500 ng and RIN of 8) was Illumina sequenced using Illumina TruSeq mRNA Library Preparation kit according to the manufacturer's instructions. RNA libraries were prepared for sequencing using Illumina TruSeq mRNA Library Preparation kit according to the manufacturer's instructions.