title: GSM6133812: RNAseq_THY1+_uSPG_Zbtb16_WT_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Purified cells were lysed in Trizol and then were purified using Direct-zol RNA mini-prep kit, including DNase I treatment to remove genomic DNA (Zymo research). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Illumina TruSeq Stranded RNA Kit with Ribo-Zero Gold Kit following manufacturer's protocols.