title: GSM6069087: i3_U_NT2_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using MN Nucleospin RNA isolation kit according to the manufacturer's instructions. Genomic DNA was isolated from cell pellets using MN Nucleospin Tissue kit according to the manufacturer's instructions. Library was prepared from the isolated genomic DNA with Illumina compatible primers using nested PCR. Initial PCR was carried out using 250x coverage input gDNA. Initial PCR products were used for second PCR with reverse index primers and forward stagger primers. Final amplicons were loaded on agarose gel, and isolated using MN Nucleospin Gel and PCR Cleanup kit according to the manufacturer's instructions.