title: RNA-seq of rat sciatic nerve crush experiment.
design: The RNA libraries were prepared using the Illumina TruSeq Stranded mRNA Sample Preparation protocol and sequenced using the Illumina HiSeq2500 platform following the manufacturer protocol. Samples were sequenced in paired-end mode to a length of 2x76bp base-pairs. Images from the instrument were processed using the manufacturer software to generate FASTQ sequence files. Read quality was assessed by running FastQC on the FASTQ files. Sequencing reads showed excellent quality, with a mean Phred score higher than 30 for all base positions.