title: Interkingdom plant-microbial ecological network analysis under different soil disturbance types in a tropical rainforest
design: Soil bacterial DNA extraction was obtained and purified. 16S rRNA gene was amplified. The polymerase chain reaction (PCR) mixture (total reaction volume, 20 L) comprised 10 ng of DNA template, 2 L dNTPs, 0.8 L of each primer, 4 L 5  FastPfu Buffer, 0.2 L BSA and 0.4 L FastPfu Polymerase (TransGen, China). The cycling conditions were 95 C for 3 min; 27 cycles of 95 C for 30 s, 55 C for 30 s, and 72 C for 45 s; and followed by 72 C for 36 min. The PCR products were purified and run using a Miseq (Illumina, USA).