title: AA0779E_HNRNPF_kd_3
design: Total RNA was isolated using Trizol (Invitrogen) and the RNeasy mini kit with on-column DNase digestion (Qiagen). Sequencing libraries were prepared from 100500ng total RNA using the TruSeq RNA Sample Preparation Kit v2 (Illumina) according to the manufacturers protocol. RNA quality was confirmed using the 2100 BioAnalyzer (Agilent) and samples were normalized and pooled for sequencing on an Illumina HiSeq2500 using barcoded multiplexing and a paired-end 75 bp length.