title: GSM5945500: ALG-1 S642A IP rep2; Caenorhabditis elegans; miRNA-Seq
library_construction_protocol: RNAs were extracted with TRIzol. RNAs were linked with Truseq LT/V1/V2 linkers using the standard 5' and 3' ligation methods. Amplicons with ~20 nt inserts were selected for sequencing. The samples were multiplexed with 8 nt indexes in the 3' linker. 5' RNA ligation and 3' RNA ligation.