title: GSM5739519: 9-mo OPNKOAD-CD11c+ rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from FACS-sorted cell pellets using RNeasy Plus Universal Mini Kit, according to manufacturer's instructions (QIAGEN). Microglial samples of 9-mo old 5XFAD and OPN-KO.5XFAD mice were processed with SMART-Seq v4 Ultra Low Input Kit for Sequencing for full-length cDNA synthesis and amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed by limited-cycle PCR to enrich and add index to the cDNA fragments. The final library was assessed with Agilent TapeStation.