title: GSM4970053: USDA110_AA_WT_3; Bradyrhizobium diazoefficiens USDA 110; RNA-Seq
library_construction_protocol: Nodules were harvested at 14 days post inoculation (dpi), frozen in liquid N2 and kept at -80°C until RNA extraction. For transcriptomic analysis, total nodule RNA was extracted as previously described in Mergaert et al., 20036, and submitted to plant and bacterial rRNA and eukaryotic mRNA depletion using RiboZero kits for plants and for Gram-negative bacteria (Epicentre, Madison, USA). Total RNA of free-living cells were extracted from bacterial pellets obtained from cultures grown in YM medium at OD600= 0.5 using hot phenol and hot SDS method according to Chapelle et al. (2015). Oriented libraries of bacterial mRNA were produced using the SOLiD total RNA-seq kit and sequenced on a SOLiD 5500XL (50bp per read).