title: Nanopore sequencing of Ferrovum dominated stalactite biofilm from acid mine drainage
design: Total genomic DNA was isolated using DNeasy PowerLyzer PowerSoil kit (Quiagen, Germany). The WGA (Whole Genome Amplification) method was used to amplify the DNA using REPLI-g Mini Kit (Qiagen) followed by T7 Endonuclease I (NEB) treatment. The sequencing libraries were prepared using Rapid Barcodig Kit SQK-RBK004 and sequenced with MinION instrument using FLO-MIN106 flowcell (Oxford Nanopore).