title: GSM4832605: Y1612231; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from frozen salivary glands samples were isolated using TRIzol® Reagent(Invitrogen) and RNA purity was checked using the Nano Photometer® spectrophotometer (IMPLEN, CA, USA). The high-quality of RNA (RNA integrity numbers (RIN) >9) for cDNA library preparations was assessed by Bioanalyzer 2100 system using the Agilent RNA 6000 Nano kit (Agilent Technologies, CA, USA). Sequencing libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) with the input material of 3μg of RNA per sample.