title: GSM6133279: H9 cells, ATAC-seq, rep 3; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were harvested using Accutase to obtain a single cell suspension and counted, followed by a wash using cold DPBS. Cells (50,000) were lysed in cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% NP-40) to obtain nuclei extracts. Nuclei were immediately used in the transposase reaction (25 μl 2× TD buffer, 2.5 μl transposase (Illumina) and 22.5 μl nuclease-free water) for 30 min at 37 °C, followed by DNA purification. Library fragments were amplified using the KAPA HiFi HotStart ReadyMix and 1.25 M of custom Nextera PCR primers. Libraries were purified using dual purification (0.5–0.7×) with SPRI AMPure XP beads and its quality assessed using a Bioanalyzer High-Sensitivity DNA Analysis kit.