title: GSM6133144: Mitral valves - FhetCko_1; Mus musculus; RNA-Seq
library_construction_protocol: The mitral valve from each mouse was dissected under the microsope in RNAlaterTM stabilization solution (Thermo Fisher Scientific; AM7021) and quickly frozen in liquid nitrogen. The mitral valve was subsequently processed individually and RNA extracted using a RNeasy Micro Kit (Qiagen; 74004). The RNA that passed quality control by the Agilent 2100 Bioanalyzer was amplified using Ovation RNA-Seq System v2 and DNA libraries were then generated using the Nextera XT DNA Library Preparation Kit. Subsequently, the samples were subjected to paired-end sequencing on an Illumina HiSeq 2500 system to a depth of 35-40 million mapped reads.