title: 16s rRNA amplicon sequencing of mus musculus: feces
design: For DNA extraction, Quick-DNA  Fecal/Soil Microbe kit column-based, bead beating step included. In  triplicate, the V4 region of the 16S rRNA gene was amplified with barcoded  PCR primers F515 and R806 modified to contain an adapter region for  sequencing on the Illumina MiSeq platform. Amplicons were verified by gel  electrophoresis, combined, purified, and sent to the UC Davis Genome Center  for library preparation and high throughput 250-bp paired-end sequencing  using the Illumina MiSeq platform.