title: Jejunum transcriptome of Treatment group, biological replicate 2
design: Total RNA was extracted from jejunum tissue using the Trizol reagent kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturers instructions. Ribosomal RNA (rRNA) was removed using a Ribo-Zero Magnetic Kit (Epicentre, Madison, WI, USA). Second-strand cDNA was synthesised using DNA polymerase I, RNase H, dNTP and buffer. cDNA fragments were purified using a QiaQuick PCR extraction kit (Qiagen, Venlo, The Netherlands) and ligated to Illumina sequencing adapters. Ligation products were subjected to size selection by agarose gel electrophoresis. Products were then PCR amplified and sequenced on an Illumina HiSeq 2500 platform. Three biological replicates were performed for each treatment.