title: GSM5236517: Whole fruit 68 DAA rep2; Capsicum annuum; RNA-Seq
library_construction_protocol: 6 fruits at 20 and 68 DAA stages were randomly selected. Whole fruits were ground using a TissueLyser system (QIAGEN) with beads at intervals of 1 min at 30 freq/s. A NucleoSpin RNA Plant kit (Macherey-Nagel) was used for total RNA extraction (following the manufacturer's recommendations) from each fruit separately and 100mg aliquots were measured from each RNA extraction and then pooled. The process was repeated with a different collection of fruits in order to obtain an independent biological replicate. RNA concentration was quantified using a Nanodrop ND-1000 spectrophotometer (Thermo Scientific Nanodrop), RNA integrity was evaluated by electrophoresis on a 1.0\% agarose gel and aliquots of RNA were run an Agilent 2100 Bioanalyzer. Sequencing of the RNA samples was performed in the Servicios Genómicos (CINVESTAV-LANGEBIO, Irapuato, México) using a Illumina NextSeq 500 to obtain at least 37 million raw paired-end reads of 150 pb per sample. RNA libraries were prepared for sequencing using standard Illumina protocols