title: B cell immunoglobulin repertoires sequencing in multiple sclerosis patients
design: we synthesized cDNA with heavy or light chain Ig targeted primers and template switch adaptor. cDNA was amplified by first semi-nested and second nested PCR amplification. We used each PCR product to prepare a sequencing library using the NEBNext Ultra II FS DNA library prep kit for Illumina (New England Biolabs). Each sample was barcoded at the library preparation step and libraries were pooled. Sequencing was performed using a paired-end 400bp x 200bp asymmetric format on an Illumina MiSeq platform