title: RNA sequencing of Hordeum vulgare genotype Conrad 4 days of germination seed biological replicate 1
design: Total RNA was isolated from seeds according to Walling et al. (2018).  RNA concentration was determined using a Nanodrop Spectrophotometer (ThermoScientific, Wilmington, DE) and the integrity was determined using the Agilent Bioanalyzer 2100 following the manufacturers protocol.  Sequencing libraries were created using the Illumina TruSeq Stranded mRNA kit with poly-A enrichment by the University of Wisconsin Biotechnology Center Gene Expression Center.  Libraries were sequenced on the Illumina HiSeq2500 sequencer using the HiSeq SBS Kit V4 by the University of Wisconsin DNA Sequencing Center.