title: WGS of E. coli ESM1.4
design: In this project we mutated E. coli Nissle 1917 derived strains using a chemical mutagen, and selected strains that had improved secretion properties from pools of several hundred mutants. This directed evolution process was carried out consecutively for several rounds. The parent strains, several related lab strains, and the selected winners from each evolution round were then selected for whole genome sequencing. DNA extraction was done using PureLink Genomic DNA Mini Kit (Invitrogen). Library preparation and sequecing was carried out by SNPsaurus (Eugene, OR, USA).