title: GSM5671183: G1s-C1-26_CUTRUN_H3K4me1; Homo sapiens; OTHER
library_construction_protocol: Chromatin samples were extracted using SimpleChIP Enzymatic Chromatin IP Kit (CST) according to the protocol. For ChIP reactions, Dynabeads anti-rabbit IgG (Thermo Fisher Scientific) was used to reduce noise instead of the Protein G magnetic beads included in the kit. CUT&RUN assay was performed using CUT&RUN Assay Kit (CST), and samples were purified using Monarch PCR & DNA Cleanup Kit (NEB). total RNA was extracted using the RNeasy plus mini kit (Qiagen). ChIP-seq and CUT&RUN libraries were prepared using KAPA HyperPrep Kit (KAPA Biosystems). Total RNA was purified by NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), and RNA-seq library was prepared by KAPA RNA Hyper prep kit (KAPA Biosystems).