title: RAT3_Ipsi_incision_12d
design: Sequencing libraries were constructed from 1 g total RNA using TruSeq Stranded Total RNA Library Prep Globin (Cat. No. 20020612, Illumina, Inc) used according to the manufacturer's instructions. Adapters were IDT for Illumina - TruSeq RNA UD Indexes. Amplification was performed using 10 cycles which was optimized for the input amount and to minimize the chance of over-amplification. Libraries were pooled in equimolar amounts for sequencing. The pooled libraries were sequenced on a NovaSeq 6000 to achieve a minimum of 46 million 150 base read pairs. The data was processed using RTA version 3.3.3. (PMID:34499774)