title: GSM5367000: RNA0288_APC73_Plate_4_41; Homo sapiens; RNA-Seq
library_construction_protocol: We index-sorted cutaneous DC and monocyte-macrophages using flow cytometry based on expression of characteristic patterns of surface markers/proteins into 96 well plates containing Lysis buffer. Single-cell cDNA libraries were prepared using the Smart-seq v2 protocol with the following modifications: (i) 1 mg/mL BSA Lysis buffer (Ambion Thermo Fisher Scientific, Waltham, MA, USA); and (ii) 200 pg cDNA with 1/5 reaction of Illumina Nextera XT kit. The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip. All samples were subjected to an indexed paired-end sequencing run of 2x151 cycles on an Illumina HiSeq 4000 system for about 1 million reads per sample.