title: Sample 51 Tumor Oxford Nanopore WGS: Sample 51T_ONT
design: "Genomic DNA was sheared to ~30-50kb using a Diagnode Megarupter. DNA was further enriched for long fragments via the Circulomics small read eliminator XL kit. DNA was prepared for Nanopore sequencing using the ONT 1D sequencing by ligation kit (SQK-LSK109). After an Ampure clean up step, prepared fragments were ligated to ONT specific adapters via the NEB blunt/TA master mix kit. The library was loaded onto a PromethION PRO0002 flowcell and was sequenced with standard parameters for 3 days."