title: GSM6253308: ESCs-derived differentiated cells, NC rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the trizol method ,according to the manufacturer's instructions. The poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Then the selected RNA was fragmented and copied into first strand cDNA, which was followed by second strand cDNA synthesis. These cDNA fragments then had the addition of a single 'A' base and subsequent ligation of the adapter. The products were then purified and enriched with PCR amplification. PCR yield was quantified and samples were pooled together to make a single strand DNA circle (ssDNA circle), which gave the final library.