title: Virome analysis reveals the wide viral spectrum of Growth Retardation Disease and normal Macrobrachium rosenbergii in China
design: The gill, hepatopancreases, and muscle tissues were homogenized, frozen, and thawed three times. The suspension of each pool was subjected to centrifugation, and the supernatant was filtered through a 0.45 m filter. RNA was extracted using the QiaAmp Mini Viral RNA kit and quantified using Qubit 4.0. The ribosomal RNA was removed by the NEBNext rRNA Depletion Kit . Sequencing libraries were generated by using RNA Library Prep Kit for Illumina(NEB, USA). Then, the 150 bp paired-end sequencing of the RNA libraries was performed using the Illumina NovaSeq platform.