title: 16S rRNA amplicon sequencing of microbiome samples obtained from ICU patients
design: We investigated the impact of two commonly used antibiotics, ceftriaxone (3GC) and piperacillin/tazobactam (TZP) on endotracheal, perineal and faecal microbiomes of intensive care patients in Australia. Patients exposed to either 3GC (n=23), TZP (n=23) or no -lactams (n=21, control group) were sampled over time and 16S rRNA amplicon sequencing was performed to examine microbial community diversity and composition.