title: GSM6069372: 2D-TEC; Homo sapiens; RNA-Seq
library_construction_protocol: Single cells were suspended in PBS supplemented with 20% FBS and sent to the Genomics Research Core at the University of Pittsburgh for QC, library preparation, and droplet sequencing on the 10X Genomics Chromium controller. Single cells (>70% viability) were washed, strained, and subjected to 10X Genomics single cell library construction using the Chromium single-cell 3' reagent v3 kit and sequencing protocol according to the manufacturer's recommendations. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)