title: GSM6131709: hiPSC-CF, control, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions, and quantified using a NanoDrop 2000c (Thermo Scientific). RNA quality was assessed using the Fragment Analyzer (Agilent). All samples passed quality control standards of minimum concentration and RNA Quality Indicator (RQN) > 9, with discrete 18S and 28S bands. cDNA libraries were generated from 500ng of total RNA using the QuantSeq 3′ mRNA-Seq Library Prep FWD kit for Illumina (Lexogen) following the manufacturer's instructions.