title: GSM6131939: spASCs-ECs coculture-DSCM, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Spinal cord slice culture system: After 12 days of cultivation, the organotypic was removed from  the hydrogel, and hydrogel with cells was digested by collagenaseand  trypsin. Digestion was neutralized with DMEM supplemented with  10% FBS. After concentration (300g, 5min), The cell pellet was washed and resuspension with  PBS supplemented with 0.04% BSA. The cell numbers and viability (>75%) were confirmed  using TC20 Automated Cell Counter. In hspASC-HUVEC co-culture system: After  five days of cultivation, the approaches used for cell digestion and suspension preparation were  identical to the Spinal cord slice culture system The cell suspension was loaded into Chromium microfluidic chips with 30  chemistry and barcoded with a 10× Chromium Controller  (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed  and sequencing libraries constructed with reagents from a Chromium Single Cell 30   reagent kit (10X Genomics) according to the  manufacturer's instructions. Sequencing was performed with Illumina  NovaSeq 6000.