title: Lung D139-RML-BPS-3_snRNA-Seq: Sample D139-RML-BPS-3
design: "Lung tissue was homogenized using a mortar and pestle in liquid nitrogen. 75,000 nuclei sorted using a SH800 (Sony). 12,000 nuclei were loaded onto a Chromium controller (10x Genomics). Libraries were generated using the Chromium Single Cell 3' Library Construction Kit v3 (10x Genomics, 1000078) according to manufacturer specifications. Libraries were sequenced using a NextSeq500 and HiSeq4000 (Illumina) using these read lengths: Read 1: 28 cycles, Read 2: 91 cycles, Index 1: 8 cycles. Raw sequencing data was demultiplexed and preprocessed using the Cell Ranger software package v3.0.2 (10x Genomics)."