title: GSM5919760: lung single cell 215; Mus musculus; RNA-Seq
library_construction_protocol: A 12 week-old female Prox1-GFP mouse was perfused with PBS to clear the blood. Subsequently, the lung was filled with 10 mg mL-1 dispase II in PBS via the trachea and incubated for 10 min at RT. Next, 1.0 mg mL-1 collagenase II in PBS supplemented with 0.2 mg mL-1 DNase I was injected into the lung lobes and lung tissue was minced with scissors in 1.5 mL of 1.0 mg mL-1 collagenase II in PBS supplemented with 0.2 mg mL-1 DNase I. Tissue dissociation was performed for 15 min at 37°