title: GSM5740139: Bone marrow derived macrophages - KO_M2_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from polarized BMDMs was extracted with the RNeasy kit (QIAGEN; cat.74106). Poly-A, strand-specific RNA library construction was performed by the Yale Center for Genomic Analysis utilizing the TruSeq Library Prep Kit 2.0 per the manufacturers specifications. . Following first-strand synthesis with random primers, second strand synthesis was performed with dUTP for generating strand-specific sequencing libraries.