title: GSM5365928: WT-1; Mus musculus; RNA-Seq
library_construction_protocol: Lysates were clarified from FACS-sorted  hematopoietic stem cells First, 30 HSCs ( Lineage- Sca-1+ c-kit+ CD34- CD150+) from Ythdf3-/- and WT mice were sorted into lysis buffer directly. The transcriptome libraries were prepared using Smart-seq2 method. Then, Agilent Bioanalyzer 2100 system (Agilent Technologies) was used to assess the insertion size, and HiSeq PE Cluster Kit v4-cBot-HS (Illumina) was used to perform the clustering of the index-coded samples according the manufacturer's instructions. Finally, the libraries were sequenced using an Illumina Hiseq platform with 150-bp paired-end.