title: WGS of Betula pendula: leaf
design: Sequencing libraries were generated using DNBseq technology. Genomic DNA was randomly sheared and fragments were size selected. Ends were polished and ligated with adapters for PCR amplification. DNBseq technology consists of DNA NanoBalls (DNBs) which are small, high-density sequencing templates. Rolling-circle replication (RCR) linear amplification makes copies of the original DNA template, instead of copies-of-a-copy. Combinatorial Probe-Anchor Synthesis (cPAS) sequencing chemistry incorporates a fluorescent probe to a DNA anchor on the DNB, followed by high-resolution digital imaging. Libraries were sequenced on a DNBseq-T7 platform with 2  150 bp reads, to a minimum read-depth of 50X for Betula and 35X for Alnus.