title: GSM5366640: Rice Near Isogenic Line GRH; Oryza sativa; RNA-Seq
library_construction_protocol: Total RNA was isolated from leaf samples, and DNA contamination were eliminated using DNase. mRNA purification kit was used to prepare the library. RNA fragments were randomly purified for short read sequencing, followed by reverse transcription of fragmented RNA into cDNA. The adapters were ligated onto both ends of the cDNA fragments. After amplifying fragments using PCR, fragments with insert sizes between 200–400 bp were selected, and both ends of the cDNA were sequenced by the read length (paired-end sequencing). RNA libraries were prepared for sequencing using standard Illumina protocols.