title: replicate  = biological replicate 2 for MCc 16SrDNA
design: The total DNA were extracted using the E.Z.N.A. Soil DNA kit . DNA quantity and purity were detected using NanoDrop2000 microspectrophotometry , and DNA integrity was assessed with 1% agarose gels. The V3-V4 hypervariable region of bacterial 29S rRNA was  1  L