title: chicken gut metagenome
design: Extracted DNA (1ng) was used as a template in amplicon PCR to target the hypervariable V3 and V4 regions of the bacterial 16S rRNA gene. The 16S metagenomics library was prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol, using the primers Fwd: CCTACGGGNGGCWGCAG and Rev: GACTACHVGGGTATCTAATCC