title: 27723_1#5
design: mRNA selection from total RNA using polyT beads. Following second strand synthesis, the double-stranded cDNA was broken down using a Covaris AFA sonicator. After fragmentation, dA-tailing, end repair and adapter addition were performed following the established DAFT-seq protocol (Chappel et al., 2020). cDNA libraries were then eluted in EB buffer and the second strand cDNA was broken down using USER enzyme mix (NEB) to produce directional libraries which were finally quantified and subjected to sequencing on the Illumina HiSeq2000