title: 16SrRNA sequencing of oral microbiome in psychologically stressed rat
design: DNA extracted from oral swab were subjected for two step PCR: Amplicon PCR with V3-V4 region primers  and Index PCR. Library quantification was done, subsequently, the library was normalized and pooled as a 4 nM library. The pooled library was denatured and mixed with PhiX Control v3 (Illumina, USA). The samples were loaded onto the Illumina MiSeq System for sequencing.