title: AsCas12a-Ultra RNP targeting AP1S3 in Jurkat cells using AP1S3-9-S guide RNA
design: Targeted loci were amplified with a single primer pair; subsequently, universal amplification was used to install unique index sequences. Illumina sequencing was carried out on a MiSeq using V2 chemistry and 2x150 paired-end reads. Primer sequences and protocols are described in the manuscript.