title: Genome sequence of C. hepaticus
design: DNA sample was quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and sample purity and integrity was checked using Nanodrop and pulsed field gel analysis respectively.  DNA library preparations, sequencing reactions, and initial bioinformatics analysis were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA). ~10kb library prep for PacBio Sequel was constructed using SMRTbell Template Prep Kit 1.0 (PacBio, Menlo Park, CA, USA). The library was bound to polymerase using the Sequel Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel using the MagBead Kit V2 (PacBio). Sequencing was performed on a single PacBio Sequel SMRT cell, using Instrument Control Software Version 5.0.0.6235, Primary analysis software Version 5.0.0.6236 and SMRT Link Version 5.0.0.6792. Reads assembled using HGAP 4 (within the SMRT Link suite) and Canu. Assembly was run using default parameters