title: Illumina HiSeq 4000 sequencing; Single-cell RNA-seq of human kidney tumors
design: Single-cell RNA-seq of human kidney tumors
library_construction_protocol: Fresh nephrectomy samples were collected from a total of 5 untreated ccRCC patients. Up to 9 punch biopsies were collected in each specimen in order to capture its heterogeneity. Out of these 5 patients, 7 samples were obtained. Two samples consisted of fresh versus frozen single cells from the primary tumor site of one patient. Two other samples consisted of matched primary and distant thrombus sites (the vena cava) of a second patient. The three remaining samples came from the primary tumor sites of three distinct ccRCC patients.  All steps for the nucleic acid extraction were carried out according to the manufacturers protocol (Chromium Single Cell 3 Library & Gel Bead Kit v2 ; 10x Genomics) All steps for the construction of the single-cell libraries were carried out according to the manufacturers protocol (Chromium Single Cell 3 Library & Gel Bead Kit v2 ; 10x Genomics)