title: GSM6133747: RCC-BM1-Involve; Homo sapiens; RNA-Seq
library_construction_protocol: 10X Genomics Single cell 3' gene expression V2 We collected unique sets of tissue fractions from each patient: solid metastatic tissue (Tumor fraction), liquid bone marrow at the site of the metastasis (Involved), liquid bone marrow from a vertebral body distant from the tumor site (Distal/Noninvolved), as well as bone marrow from hip-replacement surgery (Benign). Cells were washed once with 2% FBS-PBS (v/v) followed by DAPI staining (1 ug/ml). Flow sorting for live and non-erythroid cells (DAPI-neg/CD235-neg) was performed on a BD FACS Aria III equipped with a 100um nozzle (BD Biosciences, San Jose, CA) instrument.