title: ITS of soil fungi
design: We amplified the ITS1 region using the newly developed primer ITS1FI2, 50-GAACCWGCGGARGGATCA-30 and ITS2 (White et al., 1990). ITS1FI2 overlaps in six positions with ITS1F but is located closer to the N-terminal of the 18S. We used combinatorial primer labeling to identify samples after multiplexed sequencing.