title: GSM4832633: SD48hr2_S17: w303 Spores incubated for 48 hours in SC with 0.002% glucose, replicate no.2; Saccharomyces cerevisiae W303; RNA-Seq
library_construction_protocol: We then extracted the RNA from the leftover, un-germinated spores RiboPure Yeast Kit (Ambion, Life Technologies) as described by its protocol. Next, we prepared the cDNA library with the 3' mRNA-Seq library preparation kit (Quant-Seq, Lexogen) as described by its protocol. Next, we prepared the cDNA library with the 3' mRNA-Seq library preparation kit (Quant-Seq, Lexogen) as described by its protocol. Afterwards, we loaded the cDNA library on an Illumina MiSeq with the MiSeq Reagent Kit c2 (Illumina) as described by its protocol.