title: Tumor Mutation Burden, Expressed Neoantigens and the Immune Microenvironment in Diffuse Gliomas: Sample CL0232_T1
design: Tumor RNA was extracted from Formalin Fixed Paraffin-Embedded (FFPE) tumor sections by the Rneasy FFPE kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared by using Illumina TruSeq RNA Access Library Preparation Kit according to the manufacturer's protocol (TruSeq RNA Exome kits; Illumina, San Diego, CA, USA). The sequencing was performed on Illumina NextSeq500 (Illumina) according to the manufacturer's protocols.