title: Chaerephon pumilus fecal metagenome
design: RNA libraries were produced from each individual fecal sample using ScriptSeq v2 RNA-seq library preparation kit (Epicentre, Illumina) according to the manufacturers guidelines. Samples were DNase-treated with Promega DNase for 30 min at 37C and purified on RNeasy MinElute columns (Qiagen). Seven or eight individually and uniquely single-indexed sequencing libraries were pooled together in equimolar ratios for sequencing with paired-end reads of 100bp (PE100) on an Illumina Hiseq 2000 platform.