title: RNA-seq from inflorescence meristems (Phaseolus vulgaris PHA1037; replicate 3)
design: Total RNA was extracted using TriFast Reagent (Peqlab, Erlangen, Germany) according to the manufacturers instructions. The RNA sample quality was assessed by NanoDrop 2000C (Thermo Scientific, Wilmington, USA), agarose gel electrophoresis and Agilent 2100 BioAnalyzer (Agilent Technologies, Santa Clara, CA, USA). Only the RNA samples with 1.8/2.1 (260/280) and 2.0/2.5 (260/230) ratios and a RIN (RNA integrity number) value >7.0 were sequenced. Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The cDNA library insert sizes ranged from 250 to 300 bp. The complete libraries were purified with AMPure XP system (Beckman Coulter, Beverly, MA, USA) and qualified by Agilent Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Finally, libraries were sequenced on an Illumina Hiseq 2500 platform (Illumina Inc., San Diego, CA, USA), and 150 bp paired-end reads were generated.