title: GSM5736085: Day_0_KO2; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were purified by using Trizol (Invitrogen) and Direct-zol RNA kit (Zymo Research) according to the manufacturer's protocols. Library construction was conducted by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's protocol. Libraries were sequenced to 50 bp read length on a HiSeq2500 (Illumina).