title: replicate  = biological replicate 1 for MCs 16S rDNA
design: The total DNA were extracted using the E.Z.N.A. Soil DNA kit . DNA quantity and purity were detected using NanoDrop2000 microspectrophotometry , and DNA integrity was assessed with 1% agarose gels. The V3-V4 hypervariable region of bacterial 16S rRNA was amplified using the 338F and 806R primers. High-throughput sequencing was performed in paired-end model using an Illumina Miseq PE300 platform