title: GSM6133667: APRIL KO LPS #2; Mus musculus; RNA-Seq
library_construction_protocol: RNA from bone marrow derived macrophages was isolated using the mirVANA RNA isolation kit.  To measure the library concentration, NEBNext Ultra II Directional RNA Library Prep kit  (New England BioLabs) was used. Library construction was performed using the TruSeq RNA Library Prep Kit (Illumina) according to the manufacturer's protocols. RNA-seq was performed using the Illumina NextSeq 550 with the sequencing setting of single read 85 bp to generate ∼20 M reads per sample.