title: PacBio Genome sequencing of Bothrops jararaca: blood and liver from adult female
design: DNA samples from blood and liver of the same individual were extracted by proteinase K digestion, followed by separation on pulsed-field gel electrophoresis and electroelution using Electroeluter 422 (Bio-Rad). DNA was quantified by fluorimetry using Quant-iT PicoGreen dsDNA Assay Kit (Thermo) and aliquots of 4 ug of DNA were sent (IBAMA export license No 15BR017067/DF) to PacBio sequencing