title: GSM4832829: Serum_8hr_371880; Homo sapiens; RNA-Seq
library_construction_protocol: Cells cultured in the kidney MPS were isolated by injecting 100 uL buffer RLT (Qiagen, 79216) and the lysate was collected at the outflow port into an additional 900 uL of buffer RLT then frozen at -80°C until extraction. Total RNA was isolated using the Qiagen RNeasy Micro Kit. Total RNA was normalized to 2ng in a total volume of 9ul and then transcribed to cDNA in a dedicated PCR clean workstation using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Mountain View, CA).  Sequencing libraries were constructed from cDNA using the SMARTer ThruPLEX DNA-Seq kit (Takara Bio).  Final libraries were quantified and library insert size distribution was checked using the Bioanalyzer (Agilent Technologies, Santa Clara, CA).  Base calls generated in real-time on the NovaSeq 6000 instrument (RTA 3.1.5);demultiplexed, unaligned BAM files produced by Picard ExtractIlluminaBarcodes and IlluminaBasecallsToSam are converted to FASTQ format using SamTools bam2fq (v1.4).