title: GSM6285433: Glu 2; Streptococcus mutans; RNA-Seq
library_construction_protocol: Pellets were frozen in liquid nitrogen and mechanically crushed, RNA was harvested using Trizol reagent. Illumina ScriptSeq Complete Kit (Bacteria) was used  for the construction of sequencing libraries with input 2,5 ug of mRNA. Illumina ScriptSeq Complete Kit (Bacteria) (BB1224) - firstly total RNA was depleted with Ribo-Zero rRNA Removal Reagents, following with the steps cDNA synthesis, 3'Terminal tagging and PCR and library purification within Script-Seq v2 RNA-Seq Library Preparation Kit