title: XT_genome_illumina:TNAq_warm_M
design: MO-BIO Power Soil DNA isolation kit following the manufacturers guidelines. DNA was prepared for metagenomic analyses using the Nextera XT genome kit  following manufacturers protocol with the minor modification of increasing the PCR cycles from 12 to 15 because of low DNA yield in some of the samples. The samples were loaded at 8 pM with 2% PhiX spike-in on an Illumina MiSeq at the University of Tennessee Genomics Core on a v3, 600 cycle flow cell, reading 275 bases paired-end