library_construction_protocol: Gonads were dissected from 1–3 day old flies raised at 25°C. Ovaries were dissected from virgin females. 3 biological replicates were dissected for each genotype. Total RNA was isolated from all genotypes using RNA-bee (Tel-Test). Contaminating DNA was removed from the RNA using Turbo-DNA-free (Ambion). 200ng of RNA was used to prepare each library using the illumina TruSeq RNA Library Prep kit v2