library_construction_protocol: For mRNA-seq, poly(A) RNA was enriched from total RNA, then randon digested by alkaline buffer. For Ribo-seq, 50U/ug Rnase If was treated, then monosome was enriched before RNA extraction. RNA fragments length between 15 to 50 bases were recycled for library generation. Ribo-seq and mRNA-seq libraries were generated by a published in-house protocol (Chen and Tanaka, Cell reports 2018).