library_construction_protocol: Total RNA was extracted from the same number of cells among each group using QIAzol lysis reagent (Qiagen), and RNA was then subjected to DNase I digestion and purified using a miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA integrity was checked with a NanoDrop, and only samples with a ratio of 260/280 between 2.0 - 2.1 were subsequently used for ribosome depletion. The library preparation and RNA-sequencing were done by Novogene Corporation Inc (Sacramento, CA). The RNA-seq libraries were constructed using NEBNext UltraTM II RNA Library Prep Kit for Illumina and were sequenced by Novaseq 6000 PE150 system. Raw reads of FASTQ format were firstly processed through fastp, and clean data (clean reads) were obtained by removing reads containing adapter and poly-N sequences and reads with low quality from raw data. At the same time, Q20, Q30 and GC content of the clean data were calculated. All the downstream analyses were based on the clean data with high quality