library_construction_protocol: RNA was isolated from PTs or renal endothelial cells from 3 male mice in each experimental group, using the RNeasy Plus Micro Kit (Qiagen Inc., Toronto, Ontario, Canada). RNA quality was assessed using the Fragment Analyzer (Advanced Analytical Technologies, Inc., Ames, LA, USA). Only RNA with RQN scores of >7 was selected for further analysis Library construction was performed with the NEB Next Ultra II RNA Library Prep Kit for Illumina using the polyA mRNA workflow (New England Biolabs Ltd., Ipswich, MA, USA). For each sample, 20 ng of total RNA was used as input for the library preparation. Following library construction, the samples were pooled to minimize potential sequencing bias and run on NextSeq 500 (Illumina, San Diego, CA, USA). A total of six 75 cycle high output flow cells were run to achieve approximately 40 million reads per sample.