library_construction_protocol: Cells were synchronised by medium change. Samples were collected starting 9h after synchronisation in 3h intervals for 45h. All cells were cultured in Dulbecco's Modified Eagle Medium DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% FBS (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% Penicillin−Streptomycin (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) in a humidified atmosphere containing 5% CO2 at 37 °C Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. This extraction protocol includes the removal of genomic DNA.  mRNA libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the low sample protocol guidelines.