library_construction_protocol: Total RNA was extracted using TRIzol from cell pellets following the manufacturer's protocol. For native RNA sequencing libraries were prepared by the standard kit (SQK-RNA002, Oxford Nanopore Technologies). The libraries were loaded onto R9.4.1 flowcells (FLO-MIN106D, Oxford Nanopore Technologies) and sequenced using four MinION Mk1b devices separately in parallel (MinKNOW 4.1.2, Oxford Nanopore Technologies). For direct cDNA sequencing, Each 1.5 μg of purified total RNA with 0.1 μl of RCS from direct RNA sequencing kit (SQK-RNA002) and 0.1 ng of the SIRV set 3 (Lexogen) control was prepared as a sequencing library following manufacturers instruction (SQK-DCS109, Oxford Nanopore Technologies). ONT long-read RNA sequencing