library_construction_protocol: For each sample, 1 well of a Chromium Chip B (10x Genomics Cat No. 1000153) was loaded with 16,000 freshly dissociated cells. scRNAseq was performed using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics Cat No. 1000075) according to the manufacturer's protocol.