library_construction_protocol: After tissue collection, all specimens were preserved with RNA Fixer sample preservation solution and stored at -80℃ The amount and quality of total RNA were evaluated using Agilent 2200 instrument after total RNA were extracted using the Magen kit. According to the manufacturer's operating guide, the rRNA were extracted from the total RNA using the Vazyme Ribosome Removal Kit (Vazyme Biotech Co., Ltd). The Preparation Kit of The TruSeq Strand Total RNA Library was used to create RNA libraries utilising rRNA-depleted RNA (Illumina)