library_construction_protocol: Cells were harvested by trypsinization Neuronal progenitor cell (NPC) differentiationThe differentiation of pluripotent ESCs into NPCs was based on a protocol described before (Bibel et al., 2007). column purification SCRB-seq adapted for bulk samples (Ziegenhain, C., Vieth, B., Parekh, S., Reinius, B., Guillaumet-Adkins, A., Smets, M., Leonhardt, H., Heyn, H., Hellmann, I., Enard, W., 2017. Comparative Analysis of Single-Cell RNA Sequencing Methods. Mol. Cell 65, 631–643.e4.)