library_construction_protocol: Abdominal carcasses from 100 females of each genotype were dissected in Grace's medium supplemented with 10% fetal bovine serum (FBS; Sigma). Fat body cells were dissociated from abdominal carcasses with 500 µl dissociation buffer [0.5% Trypsin (Sigma) plus 1 mg/ml collagenase (Sigma) in 1x PBS] per 50 carcasses for 30 minutes at room temperature. Samples were gently agitated every 5 minutes to facilitate separation of cells from the cuticle. 500 µl of Grace's media plus 10% FBS was added to stop the enzymatic reaction and supernatants were placed in new tubes. Carcasses were rinsed with Grace's medium plus 10% FBS and the two supernatants per genotype were combined. Dissociated cells were centrifuged at 3.3 rpm for 5 min at room temperature. Supernatants were removed and cells were immediately lysed in 250 µl lysis buffer from the RNAqueous-4PCR DNA-free RNA isolation for RT-PCR kit (Ambion). RNA was extracted from all samples following the manufacturer's instructions cDNA library construction, Illumina sequencing, and differential expression analysis was performed by Novogene Bioinformatics Technology Co., Ltd (Beijing, China). The cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. The cDNA library for each sample was quality assessed using an Agilent Bioanalyzer 2100, and library preparations were sequenced on a NovaSeq6000 platform with PE150 read lengths.