library_construction_protocol: Total RNA fraction was isolated from cultured NPC samples with Trizol reagent (Thermo Fisher) followed by total RNA extraction using the RNA Clean and Concentrator-25 (Zymo Research). mRNA was isolated from total RNA with Dynabeads mRNA purification kit (Thermo Fisher). Libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina and sequenced in Illumina HiSeq 2500 yielding approximately 30 million 50 bp single-end reads. Illumina short-read RNA-seq