library_construction_protocol: The single-cell suspension from tumor with the viability over 90% was loaded onto the 10 × Genomics single-cell-A chip for target capture of ~10,000 cells/chip. Libraries were prepared using a Chromium Single Cell 3' Reagent Kit (10X Genomics, v3 kit) following the directions of the manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols. RNA libraries were prepared for sequencing using standard Illumina protocols 10X Single-cell RNA sequencing platform