library_construction_protocol: Total RNA was purified using GeneJET RNA purification kit (Thermo Scientific). 5μg DNase-treated total RNA was subjected to mRNA was enrichment using oligo(dT) beads (NEB) and subsequently, double-stranded cDNA libraries were generated by using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's instructions.