library_construction_protocol: Illumina MiSeq HybITS-1F_rRNA, HybITS2_rRNA, In Candida albicans SC5314 these primers create a 256 bp amplicon in ITS1 with the total amplicon with hyb regions being exactly 323 bp. The ITS-1F primer is rooted in the 18S locus while ITS2 is in 5.8S.