library_construction_protocol: Total RNA was extracted using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) and the RNeasy Plus Micro Kit (QIAGEN, Code: 74034, Germany). The total RNA and isolated mRNA quality and quantity were checked using the Agilent 2100 Bioanalyzer and NanoDrop (Thermo Fisher Scientific, MA, USA). Libraries for RNAseq were created from 1µg of mRNA from each culture.