library_construction_protocol: Total RNA was extracted using TRIzol reagent.  RNA integrity was determined using the RNA6000 Nano kit on the Agilent 2100 Bioanalyzer . RNA-seq libraries were prepared using Illumina® TruSeq RNA Library Prep Kit v2 and were sequenced using Illumina® Hiseq2500 to obtain 150-bp paired-end reads.