library_construction_protocol: total RNA was harvested using TRIzol reagent and isolated using the chloroform-isopropanol method. cDNA was transcribed using the QuantiTect Reverse Transcription Kit (Qiagen, #205311). RNA-seq was performed according to the instructions by the manufacturer (TruSeq2, Illumina) using 500ng of total RNA for preparation of cDNA libraries.