library_construction_protocol: Single cell suspensions of control and Chd4 knockdown spermatogonia were created, and an equal number of cells from each of the 3 biological replicates was combined in single 'control' or 'Chd4 knockdown' tube. Live cells were loaded into a Chromium Controller (10X Genomics, Inc.). Single-cell cDNA libraries were generated using v2 chemistry according to the manufacturer's protocol (10X Genomics, Inc.).