library_construction_protocol: freshly sorted thymic CD1-tetramer positive NKT cells from PLZFON and WT mice were digested with GenDNA Digestion Buffer and Proteinase K for 12 to 18 h at 50 °C with shaking. Genomic DNA extracted from each sample was fragmented by sonication with a Diagenode Bioruptor to produce a median fragment length of 180-230 bp. iDeal Library Preparation Kit (Diagenode)