library_construction_protocol: HUVECs were stimulated for 3hours and total RNA was manually extracted using Trizol reagent and purified by ethanol precipitation. TruSeq Stranded Total RNA LT Sample Prep Kit (Gold) was used with total RNA for the construction of sequencing libraries. TruSeq Stranded Total RNA LT Sample Prep Kit (Gold) was used for the construction of sequencing libraries and total RNA libraries were processed following standard Illumina protocols.