library_construction_protocol: Dead cells were removed using kit and cells were resuspended in PBS Cells encapsulated into droplets, and libraries were prepared using Chromium Single Cell 3′ Reagent Kits v3 (1000075) according to manufacturer's protocol (10x Genomics). The generated scRNA-seq libraries were sequenced on a HiSeq using a read structure of 28 bp for Read 1, 8 bp for Index 1, and 101 bp for Read 2. Library preparation was performed using 10x Genomics Chromium Single Cell 3' Solution with v3 chemistry following manufacturer protocols (10x Genomics).