library_construction_protocol: RNAs were isolated using the Qiagen RNeasy Mini Kit per manufacturer's instructions. the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was used and mRNA enriched using oligo(dT) beads. Constructed cDNA libraries that pass a library quality control test (consists of Qubit, LabChip fragment analysis, and qPCR steps) are sequenced on an Illumina NovaSeq 6000 PE150 platform.