library_construction_protocol: HCT116 and DKO1 DNA (1 μg) was sheared using the Covaris g-TUBE spun at 3400 x g in 2 x 60 sec spins. Sheared DNA was prepared for Nanopore sequencing using the Ligation 1D kit (SQK-LSK109) according to manufacturer's instructions. Each cell line was sequenced on one PromethION flow cell.