library_construction_protocol: The cell pellet was resuspended and homogenized in polysome lysis buffer (30 mM Tris-HCl, pH 7.5, 100 mM NaCl, 30 mM MgCl2, 1% sodium deoxycolate, 1% Triton X-100, 100 mg ml−1 cycloheximide, 1 mM DTT and 30 U ml−1 RNasin).  After centrifugation at 12,000 rpm for 15 min at 4 °C, cytoplasmic extracts with equal amounts of RNA were loaded on a 15–50% sucrose gradient and centrifuged at 4 °C for 3 h 30 min at 39,000 r.p.m in a SW41Ti Beckman rotor by using a Beckman Coulter Ultracentrifuge Optima L-90K. Gradients were read at 254 nm by the BioLogic LP system (BioRad) and the fractions (1.0 ml each) were collected for subsequent RNA extraction to isolate the polysomal RNA fractions. Total and polysomal RNA fractions were incubated with 200 ug/ml proteinase K and SDS 1% for 1 h at 37 °C. RNA was extracted by phenol/chloroform 5:1 (Sigma-Aldrich; P1944) method, followed by overnight isopropanol precipitation at -80 °C, centrifugation at 14000 r.p.m. for 45 minutes and resuspension in 10 l of DEPC water. At the end of the procedure, DNAse digestion was carried out with Turbo DNase (Thermo Fisher Scientific) for 30 minutes at 37 °C and RNA purification was performed with RNA Clean and Concentrator kit (Zymo Research). The purified RNA was measured by using Nanodrop and the quality was assessed using the Agilent 2100 Bioanalyzer. After random primer annealing, whole library preparation was performed using the Illumina TruSeq stranded total RNA with Ribozero Plus. Completed libraries were then submitted for sequencing. RNA-seq was constructed with barcodes to allow multiplexing of 12 samples per lane. Sequencing was carried out on NextSeq 500 Illumina platform.