library_construction_protocol: Tumor-bearing hemisphere were harvested, percoll gradient isolation of immune cells were conducted, CD45hi/loCD11b+ cells were sorted by FACS analysis, and RNA was harvested. Chromium Single Cell 3'V3.1 Reagent Kits (10X Genomics, Pleasanton, CA) was used for the construction of sequencing libraries. RNA libraries were generated by 10xGenomics Single Cell 3' Library Gel Bead Kit V3 and prepared for sequencing using standard Illumina protocols