library_construction_protocol: The experiment was conducted in three time-separated batches. We (mock) infected 14 days old plants and let the infection develop for either 3 or 6 days (which surround the time of the immune response activation). Plants were grown in B5 medium and the infected plants were infected with 250 nematodes. Plates were incubated in the dark at 18 degrees Celsius. Infected root segments were collected in RNAse free tubes kept in liquid nitrogen. The Maxwell® 16 Tissue LEV plant RNA kit was used for mRNA isolation with a Maxwell® AS2000 instrument (Promega), following the manufacturer's protocol. After the total RNA extraction poly(A) enrichment was performed. After fragmentation cDNA was created by reverse transcription and the library was prepared.