library_construction_protocol: Lung was removed, flash frozen on dry ice. After extracting total RNA from three randomly selected right lung tissue samples using TRIzol (Invitrogen, CA, USA), the rRNA in the total RNA was removed by a ribosomal RNA removal kit, and the RNA was interrupted to 200-300 bp fragments by ion interruption. The long  RNAs using Illumina, Poly-A enrichment, Illumina TrueSeq paired-end library construction, and short RNAs using Illumina, Poly-A enrichment, Illumina TrueSeq single-end library construction. Subsequently, using RNA as a template, the first and second strands of cDNA. After the library is constructed, PCR amplification is used to enrich the library fragments, and the library size is 300-400bp. Then, the Agilent 2100 Bioanalyzer was used for quality inspection of the library, including total concentration and effective concentration.