library_construction_protocol: RNA was extracted using QIAGEN AllPrep RNA mini kit as per manufacturer's protocol. RNA-sequencing libraries were prepared using the NuGen FFPE RNA-Seq Multiplex System (Tecan US, Inc., Morrisville, NC). Briefly, 100 ng of DNase treated RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol.Library molecules containing ribosomal RNA sequences were depleted using the NuGen AnyDeplete probe-based enzymatic process