library_construction_protocol: RNA was extracted at the specified times and conditions. For sensitive and osimertinib resistant cells, RNA was extracted from cells in culture. All extractions were performed with the RNeasy extraction kit (Qiagen, #74004) and concentrations were measured by nanodrop technology. RNA libraries for RNA-seq were prepared using TruSeq V3 SBS-Kit -HS Library Prep Kit following manufacturer's protocols.