library_construction_protocol: 50,000 to 100,000 cells were precipitated, washed with PBS and resuspended in die-cold ATAC Lysis Buffer. Cell pellet was transfered to a PCR tube on ice, span down and, supernatant removed. Cell pellet was further resuspended in a Tagmentation buffer. After tagmentation reaction, performed for 1 hour at 37 degrees celsius, the tagmented DNA was isolated and quantified by Qubit. ATAC-Sequencing was performed with help of Active Motif ATAC-Seq kit as per manufacturer instructions.