library_construction_protocol: Samples were maintained at 4C. S1 cortex was micro-dissected, homogenized and sorted for all nuclei in nuclei solubilization buffer. For scRNA-seq, S1 cortex layers were micro-dissected, homogenized in PBS with DNAse and RNAse-inhibitor, followed by FACS in BSA-containing PBS. Libraries were produced using 10x genomics technology.