library_construction_protocol: FF skin was homogenized in Trizol, and chloroform was added and the aqueous phase was separated. 400ul of the aq phase was combined witth 400ul 70% Ethanol and used  in an RNAeasy mini Kit (Qiagen, 74106) in accordance to standard manufacturer's instructions including Dnase treatment treatment procedure. Libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit.