library_construction_protocol: The mouse brain and skull tissue contains ischemic and peri-ischemic regions were harvested for RNA sequencing. Total RNA was extracted using TRIzol Reagent (Thermo Fisher Scientific Inc.) and further treated with DNase to remove genomic DNA contamination. The mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA), and was then used for RNA-sequencing library preparation with the NEBNext Ultra II mRNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA). The library was performed using Illumina sequencing with the paired-end 2 x 150 sequencing mode. RNA libraries were prepared for sequencing using standard Illumina protocols