library_construction_protocol: a percutaneous biopsy of the right vastus lateralis was performed under local anesthesia (2% lidocaine) using a modified Bergstrom needle. Muscle tissue was rapidly blotted with sterile gauze, weighed at the bedside, rapidly frozen in liquid nitrogen, and transferred to -80 freezer for storage RNA was extracted from powdered muscle biopsy samples using the Qiagen RNeasy mRNA extraction kit