library_construction_protocol: Protonemal filaments maintained on ABCD medium prior to harvest of genomic DNA. 3D defective growth mutants were generated by UV mutagenesis of protoplasts derived from the Vx::mCherry line (Villersexel strain that accumulates mCherry). Protonemal filaments maintained on ABCD medium Genomic DNA was extracted using CTAB protocol Genomic DNA fragmented, repaired, A-tailed and adaptor ligated before size selection and amplification