library_construction_protocol: RNA was extracted using Qiagen Rneasy kits. mRNA quality was assessed using the Agilent Bioanalyzer. Libraries for mRNA-seq made using total RNA and the Illumina TruSeq mRNA sample preparation kit Paired end (2x50bp) sequencing was performed on the Illumina HiSeq 2000/2500 sequencer at the UNC High Throughput Sequencing Facility