library_construction_protocol: BAT was removed, flash frozen on dry ice, and nuclei were processed using Nextera DNA library kit for ATAC analyses, and the total RNA from BAT was prepared using RNEasy kit (Qiagen) For ATAC-seq, library construction was performed using Nextera DNA library kit. For RNA-seq, strand-specific libraries were generated from 500 ng total RNA using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) Manufacturers instructions were followed for preparation of DNA libraries.