library_construction_protocol: high pure RNA Isolation Kit from Roche Life Science (11828665001) For library preparation RNA was converted to cDNA and amplified (10 PCR cycles) with the  SMART Seq v4 ultra-low RNA kit from Takara following the manufacturer´s instructions. For library preparation 10ng or the respective maximum available amount of RNA were used as input for the NEB ChIP Seq protocol following the manufacturer´s recommendations but skipping the size selection and using the high fidelity 2x PCR master mix from NEB.