library_construction_protocol: hESC lines were detached from their substrate (on feeder layer or Matrigel) with an enzymatic treatment of Acutase for 3 minutes. hAEC were detached with 0.05% trypsin/EDTA for 5 minutes.   Total RNA was extracted and DNAse I treated using Quick-RNA Miniprep Plus Kit (Zymo Research, R1058) according to the manufacturer's protocol. RNA was quantified using Qubit 2.0 (Invitrogen, USA) and quality was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Libraries were constructed using the Truseq Stranded mRNA library prep kit  according to the manufacturer's instruction (Illumina).