library_construction_protocol: DropSeq using a dolomite single-cell RNA-seq chip 2 (Dolomite, cat: 3200583) was used with 311 units/ul for the beads and cells. KAPA HiFi HotStart ReadyMix (KAPA Biosytems, cat: KK2602) and tagmentation by the Nextera XT DNA Library Preparation Kit (Illumina, cat: FC-131-1096), DASH depletion of mitochondrial DNA.