library_construction_protocol: CD4 and CD8 T cells from each donor were sorted by FACS and then frozen in RNAlater (QIAGEN) for bulk RNA sequencing. Total RNA from frozen samples was extracted using Rneasy Mini Kit (Qiagen) based on manufactuter protocols. RNA libraries were contructed with Illumina TruSeq RNA Sample Prep Kit v2 (cat. no. RS-122-2001) according to the manufacturer's protocol. Bulk RNA Sequencing