library_construction_protocol: Total RNA was extracted from rat liver tissue samples preserved with an RNA stabilization solution (Thermo scientific, USA). RNA purification was performed with RNeasy Mini Kit® (Qiagen, USA) according to the instructions provided by the manufacturer. Quality assessment for the RNA Integrity Number (RIN) was performed using Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). The stranded mRNA library was prepared with 1 μg total RNA (RIN>7). The normalized and pooled libraries were sequenced on DNBseqTM platform (MGI).