library_construction_protocol: The cells were collected into TRIzol LS (Invitrogen, #10296010) for RNA-sequencing The total RNA underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102) with 8 cycles of PCR, barcoded, and run on a NovaSeq 6000 in a PE50 run using the NovaSeq 6000 SP Reagent Kit (Illumina).