library_construction_protocol: Cells were lysed and the released mRNA was reversed transcribed using barcoded poly-dT primers. Biotin on the poly-dT primer was used to purify cDNA. Priming sites introduced over the poly-dT primers and the template switching oligonucleotide were used to amplify the cDNA. Subsequently, cDNA was fragmented using a hyperactive Tn5 transposase.