library_construction_protocol: Cells were collected, washed once with PBS. Then the cells were stained with Cell Hashing antibodies and resuspended in PBS containing 0.05% BSA to the desired concentration and immediately load on the 10X Chromium controller for the single-cell RNA-seq preparation. For single-cell library preparation, we follow the standard protocol of 10X Chromium 3' V3 kit. After the cDNA amplification, 50ng of samples were separated for the sgRNA enrichment library preparation.