library_construction_protocol: Spinal cord injury lesions (5 mm) were extracted and mechanically homogenized, myelin was removed using a percoll gradient, we enriched for myeloid cells via FACS and isolated single cells via the 10x Genomics Single Cell 3' Droplet-based single cell solution. 10x Genomics Single Cell 3' Droplet-based single cell solution, 10x Genomics Single Cell 3' library construction