library_construction_protocol: RNA was collected with RLT lysis buffer 72 h after seeding in microfluidics. RNA extraction from microfluidic channels was performed in triplicates from each condition, each replicate made by pulling together the lysates obtained from 3 channels. Libraries were prepared from 100ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina, following manufacturer protocol