library_construction_protocol: Total RNA was isolated from 20 mg frozen brain tissue using the RNAeasy Mini kit (Qiagen) coupled to an RNase-free DNase step (Qiagen). Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and the resulting RNA was used to construct sequencing libraries using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB). Libraries were multiplexed using NEBNext Multiplex Oligos for Illumina (dual index primers) and single-ended sequenced (75 bp) on the NextSeq 500 platform (Illumina) in accordance with the manufacturer's protocol.