library_construction_protocol: Total RNA was isolated using a commercial kit (Norgen) with DNAse treatment to remove genomic contaminations. Library generation was based on Quant Seq 3' mRNA-seq Library Prep kit (Lexogen) with oligo-dT priming. Sequencing was performed on a NextSeq500 ILLUMINA instrument and based on at least 5 million reads (75bp SE single end sequencing) for each sample.