library_construction_protocol: Total RNA was isolated using Trizol reagent following the protocols provided by the manufacturer. RNA libraries were prepared for sequencing according to TruSeq Stranded Total RNA Sample Prep Guide, Part # 15031048 Rev. E using TruSeq Stranded Total RNA LT Sample Prep Kit (Gold). The reagent was NovaSeq 6000 S4 Reagent Kit. Sequencing protocol was NovaSeq 6000 System User Guide Document # 1000000019358 v02