library_construction_protocol: Leaf disc (8 mm in diameter) was cut out from each infiltrated leaf (4th, 5th and 6th) and combined into a single biological sample 5 days post infiltration (dpi). Three samples were collected from each plant, frozen in liquid nitrogen and stored at  80 °C until further use.  SANYO/Panasonic growth chambers with 16 h light 8 h dark photoperiods at 21 °C under 150 μmol·m−2·s−1 light Agrobacterium suspension (OD600=0.15 or 1.5) was infiltrated with a 1 ml syringe into the abaxial side of 3 leaves (4th 6th youngest leaves) of 5 week old N. benthamiana. For agro infiltrating virus infected plants, the first and the second leaf of 3 week old N. benthamiana were rub inoculated with the corresponding virus sap and the plants were kept under standard conditions for another two weeks prior to agro infiltration. DNA was isolated by the DNeasy Plant Mini Kit (QIAGEN).  The target loci were amplified with the corresponding Illumina compatible primers, which were subsequently barcoded using the Nextera XT Index Kit V2 (Illumina).