library_construction_protocol: Total RNA was isolated from the tissues using RNA Mini Spin Column of Enzy Max LLC (Lexington, KY) according to the manufacturer's instructions. DNase (Thermo Scientific, Rockford, IL, Cat.# EN0521) was added to the first wash solution at 10µg/70µl and incubated for 15 min at room temperature to remove genomic DNA contamination RNA-seq library preparation by following the Illumina TruSeq stranded mRNA sample preparation guide