title: GSM6138443: Cochlea, postnatal 14, replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Dissection of 14 cochlear tissues from 7 mice was completed in less than 15 minutes from the time point of euthanizing the mice. The tissues were then incubated with 1 mg/ml Collagenase IV for 10 minutes at room temperature (RT). At the end of incubation, collagenase was inactivated by adding an equal volume of DMEM/F-12 media with 10% fetal bovine serum (FBS). Tissues were then gently triturated with a trimmed 200ul pipette to dissociate cells; after transferring half of the media to a new tube, tissues were then triturated with a new 200ul pipette for further dissociating the tightly connected cells. All dissociated cells were passed through multiple 40 µm strainers. Cells were lysed within GEMs generated by the 10x Genomics Chromium System. Libraries were constructed in accordance with the protocol of Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1.