title: Amplicon of Chondrocladia  robertballardi microbiome: Cantabrian Sea BT5-551-A3-Axis
design: Amplification  of V4 hyper-variable region of the 16S rRNA gene with primers 515F-Y (Parada  et al. 2016d) and 806R (Apprill et al.2015). PCR products were purified with  AgencourtAMPure XP Beads. And libraries prepared with Nextera XT DNA Library  Preparation Kit. Sequening was performed at the Natural History Museum of  London.