title: GSM5210297: MDA-MD-231 cells, IRE1-alpha KO, Thapsigargin, F4 (RNA-seq); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy kit (Qiagen #74104) performing on-column DNA digestion for 15 min. The concentration of RNA samples was determined using NanoDrop 8000 (Thermo Scientific) and the integrity of RNA was analyzed by Fragment Analyzer (Advanced Analytical Technologies). Approximately 500 ng of total RNA was used as input for library preparation using TruSeq RNA Sample Preparation Kit v2 (Illumina). The size of the libraries was confirmed using 4200 TapeStation and High Sensitivity D1K screen tape (Agilent Technologies) and their concentration was determined by qPCR based method using Library quantification kit (KAPA). The libraries were multiplexed and then sequenced on Illumina HiSeq2500 (Illumina) to generate 30M of single end 50 base pair reads.