title: GSM5369442: TRSPZ012105; Homo sapiens; RNA-Seq
library_construction_protocol: The AllPrep DNA/RNA FFPE Kit (Qiagen Cat No./ID: 80234) was used to purify genomic DNA and total RNA from 10 μm FFPET curls, according to the manufacturers instructions. RNA samples were then assessed for quality and quantity using the Qubit instrument and the Agilent Bioanalyzer to determine the degradation of the RNA samples (DV200 value). To generate the sequencing library, the hybridization-based Illumina TruSeq RNA Access method was performed according to the manufacturers instructions, with first preparation of the total RNA library and second library enrichment for coding RNA. Finally, normalized libraries were sequenced using the Illumina sequencing-by-synthesis platform, with a  sequencing protocol of 50 bp paired-end sequencing and total read depth of 25M reads per sample