title: Amplification of 16SrRNA gene of bacterial community from chemostat experiment to investigate degradation of permafrost
design: Bacterial DNA was extracted from filters using the ZymoBIOMICS DNA Miniprep Kit. Amplification of the 16S V4-V5 regions was performed using the sequence specific regions (515F-Y/926R) described in (Parada et al, 2016) using a two-step dual-index PCR approach specifically designed for Illumina instruments (Sequences provided are with already trimmed primers).