title: GSM5946896: IDlib_bubble_0min_2rep; synthetic construct; OTHER
library_construction_protocol: phenol chloroform extraction of DNA single-stranded 3' end DNA fragments were converted into double-stranded DNA by Bst polymerase (NEB Bst 2.0 WarmStart). And NGS library was prepared with NEBUltraII kit (NEBNext Ultra II DNA Library Prep Kit) for Illumina sequecing.