title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of Cryptomonas sp. under phototrophic and glucose supplemented phototrophic conditions, together with 3 dissolved organic carbon concentrations
design: RNA-seq of Cryptomonas sp. under phototrophic and glucose supplemented phototrophic conditions, together with 3 dissolved organic carbon concentrations
library_construction_protocol: After 14 days of cultivation, 50 ml of every culture were centrifugated for 5 min (2500×g, 18 °C), after which the supernatant was discarded. Cell pellets were resuspended in 700 μl of DNA/RNA Shield™ and homogenized in BashingBead™ (0.1 & 0.5 mm) lysis tubes (Zymo Research, Irvine, CA, USA) using a TerraLyzer™ Cell Disruptor. Cryptomonas sp. was grown under phototrophic conditions, glucose suplemented phototrophic conditions and 3 different dissolved organic carbon (DOC) concentratios: 1.5, 30 and 90 mg C l−1 (see materials and methods).  RNA was extracted using a Chemagic™ 360 and the Chemagic™ Viral DNA/RNA 300 Kit H96 following manufacturer's instructions (PerkinElmer, Waltham, USA). Extracted RNA was treated with DNAse I (Thermo Fisher Scientific), purified using Zymo spin-columns and quantified using a NanoDrop (Thermo Fisher Scientific). Samples were stored at −80 °C. RNA library generation and transcriptome sequencing of the selected treatments was conducted by Novogene Co., LTD (Cambridge, UK)