title: GSM5239768: W_ctrl_Mid_3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Whole ovaries were crushed in Trizol, Somatic and germ line tissue-FACS samples were sorted directly into 100-200µl of Trizol. Samples in Trizol were processed through Zymo RNA Micro-Prep kit (Zymo Research R2060) for RNA extraction. Poly A selection was done using Takara PrepX mRNA-8 (Takara 640098) protocol. cDNA libraries were prepared with Takara PrepX mRNA-8 (Takara 640096) protocol. Libraries were amplified for 15 cycles in PCR with sample specific PrepX Index primers and sequenced in 50bp single end runs on a Illumina HiSeq 2500.