title: Coral associated fungi
design: The ribosomal Internal Transcribed Spacer 2 (ITS2) region were conducted to amplify the coral-associated fungi by the primer set ITS1F and ITS2R combined with an Illumina adapter (White et al. 1990). Initial PCR amplification was achieved with the following thermal cycles: 94C for 15 min, then 35 cycles of 94C for 30 s, 51C for 30 s, 72C for 30 s, and then a final additional extension step for 10 min at 72C.