title: GSM5988831: TAGGCATG_CTCTCTAT; Mus musculus; RNA-Seq
library_construction_protocol: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on a negative selection strategy for astrocytes. Libraries were prepared during microfluidic capture and Nextera XT.