title: GSM5946819: SK_ATAC_ASA_REP3; Homo sapiens; ATAC-seq
library_construction_protocol: RNA-seq: The organoids were harvested using cold Advanced DMEM, pipetted up and down 10 times, moved to centrifuge tube, and then spun at 400 g for 5 min at 4°C. Upper media and Biomedical engineering (BME) were carefully aspirated and discarded. mRNA was then isolated from cells using the RNeasy® Plus Mini Kit (Qiagen) according to the manufacturer's protocol. ATAC-seq: Cells were  treated using the Omni-ATAC protocol17 with some modifications. Briefly, the cells were treated with lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and then suspended in the transposition reaction mix (50% TD Tagment DNA Buffer, 5% TDE1 Tagment DNA Enzyme, 33% PBS) for 30 min RNA-seq: RNA-seq libraries were prepared using Illumina mRNA TruSeq Kits as protocolled by Illumina. ATAC-seq: Libraries were prepared using the Nextera DNA CD Idx (Illumina 20018708).