title: Biochar-amended 30 Mg ha-1 Haplic Luvisol - long term response
design: Microbial genomic DNA was extracted from soil samples in duplicate, using a PowerLyzer PowerSoil DNA Isolation Kit (Qiagen, Hilden, Germany). The date of sampling was also determined as the end of incubation time. The V4 region of the 16S rRNA gene amplicons were sequenced (in pooled samples) according to 16S Illumina Amplicon Protocol EMP policy with MiSeq Illumina technology. The universal primers used for 16S rRNA (II) amplification were 515F (5-GTGYCAGCMGCCGCGGTAA) and 806R (5-GGACTACNVGGGTWTCTAAT).