title: RNA-Seq of Homo sapiens: resected PDAC tumor tissue: Sample PDAC228_R
design: "To obtain high quality data and overcome RNA degradation problem in PDAC tissue, exome capture RNA sequencing was performed. After quantifying total RNA using Quant-IT RiboGreen, samples were run on the TapeStating RNA ScreenTape to calculate DV200, a percentage of RNA fragments with over 200 nt. mRNA encoding exome was isolated from more than 100 ng of total RNA using Illumina TruSeq RNA Exome according to the manufacturer's instructions."