title: Shotgun metagenomic sequencing of sediment sample
design: 5 g of sediment was frozen until DNA was extracted using a QIAGEN PowerSoil DNA kit from 0.5 g of sediment. DNA was enzymatically sheared and tagged with adapters using the Nextera Flex kit. Libraries were pooled and seqenced on a NextSeq 550 with 4X coverage of each sample.