title: GSM5324755: rat cortical neurons_GRC327-CF-23_S24_5-Fluorouracil_0; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was collected using the Qiagen RNeasy Plus Kit. Two to three wells of identically-treated cultures were pooled, representing one sample. The experiment was repeated on two separate cultures.  A volume of 350 μL of lysis buffer with β-mercaptoethanol (Buffer RLT, Qiagen) was added (as described in RNeasy Plus Kit instructions) and mixed for complete lysis before being transferred to the next well to be pooled together. The lysate sample of pooled wells was transferred to a clean 1.5 mL RNase-free plastic tube and the homogenized lysate sample was then taken through the gDNA Eliminator spin column, centrifuged, and 350 to 600 μL 70% ethanol was added to the supernatant (as described in RNeasy Plus Kit instructions) to elute the RNA. polyA-selected RNA-seq libraries following PrepX PolyA mRNA isolation kit instructions (Wafergen)