title: MinION of synthetic poliovirus recombinant PV3-105B at passages 10-13
design: Viral RNA was extracted from pooled HeLa cell supernatants at passages 10-13, reverse transcribed and region of interest amplified by PCR. PCR amplicons were individually barcoded via additional rounds of PCR with Oxford Nanopore barcoding kit. Library was prepared and run using 2D sequencing kit.