title: RNA-Seq of Theodoxus fluviatils ecotypes exposed to different osmotic conditoions
design: pool of foot muscle preparation of 24 individuals that were collected at freshwater and brackish water locations and exposed to different osmotic conditions; isolation of total RNA by the Trizol method; generation of a normalized random primed cDNA library; sequencing mode HiSeq Rapid Rund 300 bp paired end on an Illumina HiSeq2500