title: GSM5368952: DMSO_HUVEC_ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: 50.000 HUVEC were used for ATAC library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). The cell pellet was resuspended in 50 µL PBS and mixed with 25 µL TD-Buffer, 2.5 µL Tn5, 0.5 µL 10% NP-40 and 22 µL H2O and incubated at 37°C for 30 min followed by 30 min at 50°C together with 500 mM EDTA pH8.0. 100 µL of 50 mM MgCl2 was added for neutralization. Purification of DNA fragments was done with the MinElute PCR Purification Kit (Qiagen). 50.000 HUVEC were used for ATAC library preparation using Tn5 Transposase from Nextera DNA Sample Preparation Kit (Illumina). The cell pellet was resuspended in 50 µL PBS and mixed with 25 µL TD-Buffer, 2.5 µL Tn5, 0.5 µL 10% NP-40 and 22 µL H2O and incub The cell pellet was resuspended in 50 µL PBS and mixed with 25 µL TD-Buffer, 2.5 µL Tn5, 0.5 µL 10% NP-40 and 22 µL H2O and incubated at 37°C for 30 min followed by 30 min at 50°C together with 500 mM EDTA pH8.0. 100 µL of 50 mM MgCl2 was added for neutralization. Purification of DNA fragments was done with the MinElute PCR Purification Kit (Qiagen). Amplification of Library and Indexing was performed as descried elsewhere (Buenrostro JD, Wu B, Chang HY, Greenleaf WJ. ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol. 2015).