title: GSM5989666: AMO_morpholino_U1_TEX_2.fastq.gz; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from U2OS and HEK293 cells using QIAzol Reagent (Qiagen; 79306) and Trizol reagent for HeLa cells (15596-018, Ambion life technologies) according to the manufactures protocol followed by DNase I treatment.  PolyA selected RNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB; E7490) for U2OS and HEK293 and Oligotex kit (QIAGEN) for HeLa cells and further processed with the NEBNext Ultra Directional RNA Library Prep Kit (NEB; E7420S) for U2OS and HEK293 or SMARTer® Stranded RNA-Seq Kit (Takara; 634839) and illumina Truseq Stranded mRNA Library Prep kit.  for HeLa cells or 3′–3′-mRNA-Seq Library Prep Kit (lexogen; 015UG009V0211).