title: GSM5242020: ETOH_7DPF_1; Danio rerio; RNA-Seq
library_construction_protocol: RNA was extracted in TRIzol (Life Technologies) from whole pooled (30 individuals/sample) larvae and purified using the Qiagen RNeasy Mini Kit. RNA quality was verified on the Agilent Bioanalyzer and DNA contamination was removed with the TURBO DNA-free (Invitrogen) kit. Single-end NextSeq Series High-Output RNA sequencing was performed on poly-A selected coding mRNAs at the Dana Farber Center for Cancer Computational Biology.