title: GSM6255161: H9_Control_Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the Qiagen RNeasy kit (Qiagen, 74106) with on column Dnase digestion (Qiagen, 79256). RNA-sequencing was performed by the Molecular Biology Core Facilities at Dana-Farber Cancer Institute. Libraries were prepared using KAPA mRNA HyperPrep (Roche, KK8581) strand specific sample preparation kits from 200ng of purified total RNA according to the manufacturer's protocol on a Beckman Coulter Biomek i7. The finished dsDNA libraries were quantified by Qubit Fluorometer (Thermo Fisher) and 4200 TapeStation system (Agilent, G2991BA). Uniquely dual indexed libraries were pooled in an equimolar ratio and shallowly sequenced on an Illumina MiSeq to further evaluate library quality and pool balance. The final pool was sequenced on an Illumina NovaSeq 6000 targeting 40 million 150 bp read pairs per library at the Dana-Farber Cancer Institute Molecular Biology Core Facilities.