title: Illumina HiSeq 3000 paired end sequencing; Regulatory function of Pja2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells
design: Regulatory function of Pja2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells
library_construction_protocol: Gene expression profiling analyses by high throughput sequencing were performed on mRNA samples isolated from the sense Pja2 in HT-22 cells (Pja2/+) and the wildtype cells. HT-22 were routinely maintained in the conventional mouseEagle's medium RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2  was used with total RNA for the construction of sequencing libraries. RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2  was used with total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard BGISEQ500 protocols