title: GSM5208242: ATAC-seq 48hours BM rep2; Mus musculus; ATAC-seq
library_construction_protocol: 48 hours after doxycycline addition, 50000 MEF nuclei were isolated as previously reported (Buenrostro et al., 2015), with modifications described in (Mayran et al., 2018). Isolated nuclei underwent transposase reaction: 2.5μl of 10x TD buffer, 10μl of water, and 12.5μl of enzyme (Illumina Nextera kit; FC-121–1031). DNA was purified with GeneRead Purification columns (Qiagen), enriched by PCR, and purified again with GeneRead Purification columns before being sequenced.