title: GSM6287804: 924E iExo-Scr#2 treated mouse; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with Trizol (Invitrogen) and cleaned up using a RNeasy extraction kit (Qiagen). RNA concentration was measured by NanoDrop, and RNA quality was assessed using Bioanalyzer RNA 6000 Nano assay (Agilent). RNA quality was assessed using Bioanalyzer RNA 6000 Nano assay. Samples with a RNA integrity number score ≥7.2 were submitted for sequencing to the MD Anderson Cancer Center Sequencing and ncRNA core using Illumnia Next Seq 500 was used for the sequencing. Library construction and Illumina-ready sequencing library generation were prepared by following the manufacturer's guidelines. cDNA concentration was estimated using High Sensitivity dsDNA Qubit kit and quantified using HS DNA Bioanalyzer. Following capture and lysis, cDNA was synthesized and amplified to construct Illumina sequencing libraries and sequenced with Illumina Nextseq 500.