title: Illumina HiSeq 2500 paired end sequencing; RNAseq evaluation of nicotinamide seed treatment analysed in Norway spruce seedlings
design: RNAseq evaluation of nicotinamide seed treatment analysed in Norway spruce seedlings
library_construction_protocol: Seedlings were harvested after three months and snap frozen (-80 C nitrogen). Roots were grounded into a powder for RNA extraction.  Seeds were planted in wet peat and kept at 23 C in the dark until germination after one week. Growth conditions for the plants until harvest at three months:  23 C, 18/6 h light/dark, fluorescent lighting tubes (150 µmol m-2 s-1). Control samples: Norway spruce seeds, collected in the orchard of Öhn (origin 57° 00´ N, alt. 55 m) were treated in a water solution of Tween 80 for 4 h with gentle shaking at 23 C in the dark.  Total RNA was extracted using Qiagen RNeasy Plant Mini Kit  Illumina TruSeq Stranded mRNA Library Preparation Kit