title: RNA-seq of Pinus sibirica: seedling 2
design: From needle sample was isolated RNA by Spectrum Plant Total RNA Kit (Sigma-Aldrich) with On-Column DNase I Digestion Set (Sigma-Aldrich). rRNA depletion was done by Ribo-Zero rRNA Removal Kit (Plant Leaf) (Illumina). DNA library were constructed by SENSE Total RNA-Seq Library Prep Kit (Lexogen).