title: GSM5484671: evoA1-seBE4max-T2A-NoRapamycin_Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using Direct-zolTM RNA Miniprep Plus kit (Zymo Research #R2072) following the manufacturer's protocol. Total RNA was analyzed for quality using the RNA 6000 Nano Bioanalyzer kit (Agilent). Only RNA with an RNA integrity number (RIN) ≥ 8 was used for subsequent library construction. RNA-seq was performed on 0.5-1.0 μg of total RNA according to the Genewiz Illumina Hi-seq protocol for poly(A)-selected samples (2 × 150 bp pair-end sequencing, 350M raw reads per lane).