title: DNA-Seq of Patrinia rupestris: leaf
design: Fresh Dioscorea opposita leaves were collected and covered with tin foil, frozen by liquid nitrogen, then kept in a -80 preservation reserve. The CTAB method was used to obtain the whole genomic DNA. The ND-2000 spectrometer (Nanodrop Technologies, Wilmington, DE, USA) was used to test the concentration of DNA. The library of 250 bp shotgun was sequenced by the Illumina X ten platform.