title: GSM5708561: human  nevus sebaceous-03; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted by Trizol reagent (Invitrogen, California, USA) from frozen  tissues according to the manufacturer's standard protocol. The NanoDrop ND2000  (Thermo Fisher Scientific Inc, Waltham, MA) instrument was used to quantify total  RNA and standard denaturing agarose gel electrophoresis was used to evaluate the  quality of the RNA. An RNA Sequencing library was conducted by Reagent test kit according to the manufacturer's instructions. The RNA Sequencing library was assessed using Agilent 2100 Bioanalyzer (Agilent Technologies Inc, Santa Rosa, CA) and quantified by quantitative polymerase chain reaction (qPCR). Illumina NovaSeq 6000 was applied for sequencing.