title: PromethION sequencing of B. napus: leaves G3D001
design: High-molecular-weight (HMW)-DNA was extracted following the protocol describe by Chawla et al. 2021. DNA quality and length were evaluated with a Nanodrop spectrophotometer (Thermo Fisher), a Qubit 2.0 fluorometer (Thermo Fisher) and gel electrophoresis. Libraries were prepared using SQK-LSK109 ligation sequencing kits (Oxford Nanopore Technology) and were afterwards loaded on Oxford Nanopore R9.4.1 flow cells