title: Sequel sequencing; Raw reads: DE01087_REF_IsoSeq_q20_flnc_reads_pollen
design: RNA was extracted from five different tissues (leaf, flower, anther, pollen, root). To ensure a high RNA quality for long-read sequencing, we performed the protocol of Acosta-Maspons et al. (2019), which is a CTAB based method for high-quality total RNA applications from different plant tissues. The remaining DNA was removed with the TURBO DNA-free Kit (Invitrogen), designed for optimal preservation of RNA during the DNase treatment. The quality check on the Agilent Bioanalyzer 2100 (Agilent Technologies) with a RNA Nano 6000 Chip resulted in RIN Scores higher than 7.6 for all tissues. The IsoSeq libraries were prepared following the PacBio protocol for Iso-Seq Express Template Preparation for Sequel and Sequel II Systems (P/N 101-763-800 Version 02; October 2019, Pacific Biosciences, California, USA). The cDNA was amplified in 12 cycles and purified using the standard workflow for samples primarily composed of transcripts centered around 2 kb.