title: GSM6290490: LPS 100ng/mL, donor A; Homo sapiens; ATAC-seq
library_construction_protocol: Immediately after lysis, nuclei were spun at 500g for 10 minutes at 4°C Resuspended the nuclei with Tn5 transposase reaction mix (25 µl 2X TD buffer, 2.5 µl Tn5 transposase, and 22.5 µl nuclease-free water) (Illumina Inc). The transposition reaction was performed at 37°C for 30 minutes. Immediately after the transposition reaction, DNA was purified using ZYMO DNA Clean & Concentrator kit. Indexed libraries were generated using NEBNext® High-Fidelity DNA library prep PCR mix (New England Biolabs) and sequenced on an Illumina NovaSeq