title: GSM5708863: RNA-Seq DBA55 mEpiSCs BioRep698; Mus musculus; RNA-Seq
library_construction_protocol: mEpiSCs were lifted with collagenase (to avoid feeder contamination) and resuspended to single cells with accutase. RNA was extracted using trizol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 129-500ng of total RNA with RIN values of 9.5-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on a NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S4 Reagent Kit (200 Cycles) (Illumina). An average of 36 million paired reads were generated per sample and the percent of mRNA bases per sample ranged from 84% to 89%.