title: Illumina HiSeq 2500 paired end sequencing; Genetic and physiological mechanisms underlying flower induction in apple
design: Genetic and physiological mechanisms underlying flower induction in apple
library_construction_protocol: Apple buds from 2-year-old spurs were collected from the trees with (ON) and without (OFF) crop load. Field-grown apple trees at Lake Constance near Ravensburg, Germany. Two treatments: Flowers removed (OFF-trees)and natural crop load (ON-trees). RNA extraction was performed using the InviTrap Spin Plant RNA Mini Kit (Invitek Molecular GmbH, Berlin, Germany) according to the standard protocol. Library of mRNA was prepared using polyA enrichment as commercial solutions for ribosomal depletion.