title: GSM5369980: TestesDKO512; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from frozen tissues following standard Trizol chloroform protocol (Invitrogen, cat. number 15596026) and aqueous fractions were then taken directly into Qiagen RNA plus extraction kit (Qiagen, cat. number 74136) for maximum purity. Total RNA quality and quantity were tested on an Agilent Bioanalyzer 2100 System (Agilent cat. number G2939BA) using RNA Nano Chip On-Chip Electrophoresis trays for samples with RIN scores greater than 8 prior to processing for library prep/construction and sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols