title: GSM5239702: RRMS4_CD4; Homo sapiens; RNA-Seq
library_construction_protocol: CD4+ T cells isolated from peripheral blood mononuclear cells (PBMCs) of RRMS patients and healthy controls. Total RNA was extracted using the Trizol isolation kit. Library construction for RNA sequencing was performed with 1 ug of total RNA using Illumina's TruSeq Stranded Total RNA LT Sample Prep Kit with Ribo-Zero Globin (Illumina, San Diego, CA, USA). Qualified samples were first proceeded to random fragmentation, with a median length of 150 bp, and converted to double-stranded cDNA. The adapter-ligated fragments were PCR amplified and gel purified. Then, the library was loaded into a flow cell, and cluster generation was executed. Sequencing was done by Illumina Novaseq6000 following the manufacturer's protocol. Image analysis, per-cycle base calling, and quality score assignment was performed with Illumina primary analysis software, RTA (Real-Time Analysis).