title: GSM4838833: PRDM16 ChIP-Seq replicate 2; Mus musculus; ChIP-Seq
library_construction_protocol: PRDM16 ChIP-seq was carried out using dual chromatin crosslinking with PFA and EGS, followed by sonication and antibody precipitation. For library preparation, genomic DNA was purified, end repaired, ligated with barcoded adaptors, amplified for 14 PCR cycles and purified using the Ovation Ultralow System V2 (NuGEN). Library fragments in the range of 100-800 bp were size-selected using agarose gel electrophoresis followed by DNA gel extraction and purification.