title: EH2 seed transcriptome from 15 days after pollination pods
design: RNA was isolated from the dissected seeds from 15 days after pollination pods using Spectrum Total RNA isolation kit (Sigma). Library was developed from high quality RNA samples using NEBNext UltraTM II RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturers protocol and were sequenced on Illumina platform (HiSeq 10X) (Illumina) to generate 150nucleotidelong pairedend (150bp x2) sequence reads.