title: Exome seq of T47D-WT cell line
design: Genomic DNA was isolated using the EasyPureGenomic DNA Kit (TransGen Biotech), following the manufacturer's instructions. Quality control of DNA samples included agarose gel electrophoresis and DNA quantification using the QuantiFluor dsDNA System and Quantus equipment (Promega). 1.0 g of genomic DNA per sample was used as input material for library preparation. For WES, libraries were generated using the Agilent SureSelect Human All ExonV6 kit (Agilent Technologies), following the manufacturer's recommendations. The synthesized sequencing libraries were purified using the AMPure XP System (Beckman Coulter) and quantified using the Agilent High Sensitivity DNA Kit (Agilent Technologies) and the Agilent Bioanalyzer 2100 System (Agilent Technologies). NGS sequencing runs were made in Illumina Novaseq systems as an outsourced service and 150bp pairedend reads were generated.