title: Sanger Sequencing of Amplicon from Severe acute respiratory syndrome coronavirus 2 heart isolate
design: Sequencing of samples was performed by ACTGene Anlises Moleculares Ltd. (Center for Biotechnology, UFRGS, Porto Alegre, RS, Brazil) using an AB 3500 Genetic Analyzer automatic sequencer equipped with 50 cm capillaries and POP7 polymer (THERMO FISHER SCIENTIFIC, WALTHAM, MA, USA). Sequencing data were collected using Data Collection 3 software (THERMO FISHER SCIENTIFIC, WALTHAM, MA, USA) and the resulting Data Collection files (.ab1; electropherograms) were converted into FASTA files (.seq; text) by Sequence Analysis Software v.6 (THERMO FISHER SCIENTIFIC, WALTHAM, MA, USA) with standard parameters. Using Clustal omega software, the FASTA files were aligned along with the complete Sars-CoV-2 genome sequences that follows: China (MT079844.1), Italy (MT890669.1), United States (MT642254.1), Russia (MT890462.1) and Brazil (MT827074.1). After aligned the sequences was blasted with blast against the genome sequences.