title: GSM5369657: FSL-R8-5963_rep3_planktonic; Listeria monocytogenes; RNA-Seq
library_construction_protocol: Following incubation, coupons were washed three times with sterile PBS to remove non-adherent cells, then immediately mixed with 1:10 volume ice-cold 10% acidified phenol:chloroform (Life Technologies, Grand Island, NY) in ethanol. Biofilm cells were detached from the coupons in stop solution using a spatula and subsequent vortexing. Cells were pelleted (15,300 x g, 20 min, 4°C), coupons removed, and pellets re-suspended in 1 mL TRIzol® (Life Technologies). RNA extraction proceeded as previously described in biological triplicate (Pleitner and others 2014; Raengpradub and others 2008). Sample quality was assessed via the 2100 Bioanalyzer system (Agilent, Foster City, CA). DNA removal was evaluated  via qRT-PCR as previously described (Pleitner and others 2014) RNA libraries were prepared using the Epicentre ScriptSeqTM complete kit (Illumina) and ScriptSeqTM Index PCR primers (Illumina)