title: Illumina HiSeq 2500 sequencing; Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.
design: Transcriptomic analysis of undifferentiated and differentiated NMR OPCs.
library_construction_protocol: OPCs were isolated from NMR pups at postnatal day 2, passed once after 14 days, and the RNAs were collected using Trizol 5 days after the passage. Each sample was collected from a different litter. RNA from the differentiated NMR OPCs was collected 7 days after inducing differentiation. The RNA samples were extracted using Trizol reagent. RNA samples were sent to the Genomics Research Center of the University of Rochester for RNA-Seq. The TruSeq RNA Sample Preparation Kit V2 (Illumina) was used for library construction.