title: Illumina HiSeq 2000 paired end sequencing of SAMD00327169
library_construction_protocol: 100 ng of genomic DNA extracted using commercial extraction kits was digested with EcolI and HaeIII in 96-well plates. Sample-unique barcode adaptors were designed and ligated onto the cut ends of the restriction sites, and the samples were then pooled into libraries of 24 individuals each. The DNA fragments (400 600 bp) were purified on a 1% agarose gel and columncleaned employing a PCR purification kit (NEB, USA). The final products were amplified for 12 cycles using Phusion DNA polymerase (NEB, USA). PCR products were purified using a PCR purification kit (NEB, USA) and quantified on a Bioanalyzer 2100 (Agilent, USA). Finally, pooled libraries were adjusted to 10 nmol and sequenced on a lane using the Illumina HiSeq platform (Illumina, USA) with 150-bp pairedend reads.