title: MinION of synthetic poliovirus recombinant 105B at passages 8-11
design: Viral RNA was extracted from pooled HeLa cell supernatants of passages 8-11, reverse transcribed and region of interest amplified by PCR. PCR amplicons were individually barcoded via additional rounds of PCR with Oxford Nanopore barcoding kit. Library was prepared and run using 2D sequencing kit.