title: RNA-Seq of Nicotiana benthamiana leaf tissue
design: For high-throughput sequencing, RNA samples treated with RNase-free DNAse I (ThermoFisher Scientific) and devoid of contaminating genomic DNA were used. Depletion of ribosomal RNA was carried out with Ribozero Plant kit (Illumina), cDNA synthesis was carried out with Illumina TruSeq Stranded Total RNA kit using random primers. The quality of cDNA libraries was verified on Fragment Analyzer, cDNA quantity was estimated by qPCR. cDNA libraries were sequenced on Illumina NovaSeq 6000 producing 100 nucleotide-long single reads.