title: Total RNA-seq of Drosophila simulans testes
design: Testes from 20 males per replicate, aged 3-5 days were dissected into cold Ringer's solution, transferred to 350l of cold lysis buffer, and then stored at -80C. RNA was extracted using the Nucleospin RNA XS isolation kit (Macherey-Nagel). Sequencing libraries were prepared using the TruSeq Stranded Total RNA kit with 150bp inserts.