title: GSM6288602: RNA_8C_WNC_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Smart-seq2 libraries were prepared as previously described (Picelli et al., 2014). Embryos or cells were lysed in hypotonic lysis buffer (Amresco, M334). After pre-amplification step, cDNAs were purified using AMPure XP beads and then subjected to library preparation using TruePrep DNA library Prep Kit (Vazyme, TD502). All libraries were sequenced by Illumina Hi-Seq Xten or Novaseq 6000 according to the manufacturer's instructions. For ERCC spike-in Smart-seq2, mESCs and embryos are quantified by mouth pipette and 1 μL 1:2000 diluted ERCC RNA Spike-In Mix (Invitrogen, 4456740) were added before cell lysis.