title: RNA-Seq of Prokoenenia wheeleri
design: We isolated total RNA using TRIzol reagent (Invitrogen) and purified mRNA with Dynabeads (Invitrogen). We assessed the quantity and quality of mRNA with the 2100 BioAnalyzer (Agilent). For cDNA library construction, we used the Illumina TruSeq kit following the manufacturer's protocols.