title: GSM6287790: P7 cerebellum Eed-deleted rep3; Mus musculus; RNA-Seq
library_construction_protocol: Brains were cut in half sagittally and drop-fixed in 4% paraformaldehyde for IHC, and half cerebellum or tumor collected for Drop-Seq.Cells were dissociated using the Papain Dissociation System (Worthington Biochemical) according to the manufacturer's protocol, as in our prior studies. https://www.nature.com/articles/s41467-019-13657-6#Sec18. Drop-Seq 3' biased cell-indexed cDNA libraries were prepared according to the Drop-seq protocol V3.1 (ref. 32), with full details available online (http://mccarrolllab.com/dropseq/). Cell and bead concentrations were both set to between 95 and 110/μL.  to high-throughput sequencing facility that does their own QC before running samples.  cDNA libraries were prepared for sequencing using standard Illumina protocols (Multiplexed samples using nextera indexes so that libraries could be pooled)