title: Whole Exome Sequencing of Homo sapiens; adult male: Sample Left_Axillary_Lymph_Node_#1_DNA
design: "Following pathologic review of all samples to confirm presence of tumor or normal tissues (in the case of nonadjacent matched normal, defined as >1 cm from the tumor margin), samples were submitted for nucleic acid isolation. Deidentified snap-frozen (SF) and formalin-fixed, paraffin-embedded (FFPE) tissue samples were sent to the UNC Lineberger Comprehensive Cancer Center Translational Genomics Lab for nucleic acid isolation using the Maxwell 16 MDx Instrument (Promega, AS3000). DNA was extracted from approximately 10 mg of SF tissue using the Maxwell 16 Tissue DNA Purification kit (Promega, AS1030) and from FFPE slides using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, AS1135) following the manufacturer's protocols (Promega, TM284 and TM349, respectively). DNA quality was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, ND-2000C) and a TapeStation 4200 (Agilent, G2991AA). DNA concentrations were quantified using a Qubit 3.0 fluorometer (Life Technologies, Q33216). WES was run on DNA samples following library preparation with an Agilent SureSelectXT2 All Exon V6 kit and performed at 200x coverage. WES were performed on an Illumina NovaSeq 6000."