title: RNA-seq of  Mtnr1a knockdown BV2 RNA-2
design: Next generation sequencing library preparations were constructed according to the manufacturers protocol (NEBNext Ultra RNA Library Prep Kit for Illumina). Then libraries with different indices were multiplexed and loaded on an Illumina HiSeq instrumentaccording to manufacturers instructions (Illumina, San Diego, CA, USA). Sequencing was carried out using a 2x150bp paired-end (PE) configuration; image analysis and base calling were conducted by the HiSeq Control Software (HCS) + OLB + GAPipeline-1.6 (Illumina) on the HiSeq instrument