title: GridION sequencing
library_construction_protocol: RNA was transcribed into cDNA using random hexamers (Thermo Fisher) and SuperScript IV (Thermo Fisher) and dsDNA was generated using Klenow (NEB). For Nanopore sequencing the �Low input genomic DNA� with PCR kit was used following the manufacturer�s instructions, (SQK-PBK004, Nanopore) apart from an additional wash step that was performed after adapter ligation.