title: GSM5368925: MLNVenus_pos_4; Homo sapiens; RNA-Seq
library_construction_protocol: hMLN-Venus organoid samples showing Venus expression were dissociated to single cells, and stained with DAPI and DRAQ5. Live cells (DAPI-, and DRAQ5+) were FACS sorted into 2x populations (Venus+, and Venus-). RNA extraction and isolation were performed using RNAeasy Micro Plus kit (Qiagen). RNA concentration and quality (RIN 7-9) were assessed using RNA6000 Pico Kit and Bioanalyser 2000 (Agilent). The library was constructed with using the SMARTer Stranded Total RNA-Seq v2 Pico Input Mammalian kit (Takara Bio).