title: Illumina HiSeq 2500 paired end sequencing; Gene expression profiling of core glioma stem cells: a novel glioblastoma cell population with diminished antigen presentation
design: Gene expression profiling of core glioma stem cells: a novel glioblastoma cell population with diminished antigen presentation
library_construction_protocol: Accutase was used to split GBM-DCs. GBM-DCs were obtained from the Human Glioblastoma Cell Culture biobank (HG3035). They are cultured on dishes coated with polyornithine  10 l/ml and laminin 10 l/ml in Neurobasal and DMEM/F12 media with N2 and B27 supplements and human recombinant FGF2 and EGF 10 ng/ml RNA was obtained using the EZNA Total RNA Kit I Samples were processed using the TruSeq RNA Sample Prep v3 kit (Illumina) according to the manufacturers instructions.