title: Illumina HiSeq 2500 paired end sequencing; RNA sequencing of the hepatic stellate cell line LX-2 treated with either DMSO or Aramchol after 24 or 48 hours.
design: RNA sequencing of the hepatic stellate cell line LX-2 treated with either DMSO or Aramchol after 24 or 48 hours.
library_construction_protocol: LX-2 cells (150,000/well) were seeded in a 6-well plate. After overnight culture in starvation media cells were incubated with either 10 uM Aramchol or DMSO vehicle in starvation media for either 24 or 48 hours. Cells were harvested by scraping.  LX-2 cells, an immortalized human HSC line was serum-starved overnight to quiesce and synchronize metabolic activity in serum-free Dulbecco's Modified Eagle Medium (DMEM - Thermo Fisher Scientific, MA) supplemented with 0.1% BSA and no antibiotics ('starvation media') at 37° C in 5% CO 2 before Aramchol incubation. LX-2 cells (150,000/well) were seeded in a 6-well plate. After overnight culture in starvation media cells were incubated with either 10 uM Aramchol or DMSO vehicle in starvation media for either 24 or 48 hours. RNA was extracted using RNeasy Mini Kit Sequencing libraries were made with the TruSeq Stranded mRNA Library Prep Kit by manufacturer's suggestions.