title: Amplicon of Chondrocladia robertballardi microbiome:  Cantabrian Sea BT6-602-E3-Stem
design: Amplification of V4 hyper-variable  region of the 16S rRNA gene with primers 515F-Y (Parada et al. 2016d) and  806R (Apprill et al.2015). PCR products were purified with AgencourtAMPure XP  Beads. And libraries prepared with Nextera XT DNA Library Preparation Kit.  Sequening was performed at the Natural History Museum of London.