title: NextSeq 500 sequencing; Genome-wide analyses of XRN1-sensitive targets in osteosarcoma cells identifies disease-relevant transcripts containing G-rich motifs
design: Genome-wide analyses of XRN1-sensitive targets in osteosarcoma cells identifies disease-relevant transcripts containing G-rich motifs
library_construction_protocol: SAOS-2 cells treated with an siRNA to XRN1 or a scrambled control siRNA were harvested and immediately snap frozen in liquid nitrogen 3x10^5 SAOS-2 cells were treated with 20pmol siRNA to either XRN1 or a scrambled control for 48 hours prior to harvesting RNA extraction was performed using a Qiagen miRNasy micro kit including an on column DNase digestion Library preparation was performed with the Illumina TruSeq kit following rRNA depletion