title: Illumina HiSeq 2500 paired end sequencing; RNA-seq from human patient derived xenografts from pancreatic adenocarcinoma patients: RS10
design: RNA-seq from human patient derived xenografts from pancreatic adenocarcinoma patients: RS10
library_construction_protocol: Heterotopic pancreatic cancer tumors were implanted in immunodeficient mice. Tissues from hPDX samples were collected and RNA was isolated. RNA was isolated using the Qiagen RNA Micro Kit. RNA sequencing was performed using PolyA selection and the Illumina TruSeq Stranded mRNA library preparation kit.