title: Illumina MiSeq paired end sequencing
library_construction_protocol: RNA was transcribed into cDNA using random hexamers (Thermo Fisher) and SuperScript IV (Thermo Fisher) and dsDNA was generated using Klenow (NEB). For Illumina sequencing the KAPA HyperPlus Kit (Roche) was used with the following modifications. The adapters were 1:10 diluted and an extra AMPure beads (Beckman Coulter) wash step was performed after adapter ligation.