title: GSM5369065: media-24-ND4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA sample integrity was assessed using automated capillary electrophoresis on a Fragment Analyzer (Agilent) using the HS RNA Kit (15NT). For all samples RQN > 8.0, a total RNA amount of > 75 ng was used as input for library preparation using the TruSeq Stranded mRNA Library Preparation Kit (Illumina). Sequencing libraries were quantified by real-time PCR using the KAPA Library Quantification Complete kit (Roche) and assessed for size distribution and absence of free adapters and adapter dimers on a Fragment Analyzer. Sequencing libraries were pooled and sequenced on a HiSeq 3000 System (Illumina) using a HiSeq 3000/4000 PE Cluster Kit and SBS Kit (150 cycles) with run conditions of paired-end reads at 75 bp length.