title: Shotgun metagenomic sequence of a fermented vegetable product (kimchi or sauerkraut)
design: We used the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs) and followed the manufacturers instructions for low input (<100 ng) libraries. For each product sampled, we fragmented ~ 100 ng of DNA (in 26 L volume) for 20 minutes using the NEBNext Ultra II FS Enzyme Mix. This fragmented DNA was then used for Illumina adapter ligation and PCR enrichment using 8-based index primers (NEBNext Multiplex Oligos for Illumina). Libraries were sequenced at the Tufts University Core Facility on a NextSeq 550 using paired-end high-output with 150 cycles.