title: Coral associated bacteria
design: To amplify the bacterial 16S ribosomal RNA gene from purified metagenomic DNA, hypervariable regions V5 and V6 of ribosomal DNA was generated by the 338F and 806R  universal bacterial primers, containing the complementary adaptor sequences for the Illumina MiSeq platform. The PCR amplification profile consisted of an initial denaturation of 15 min at 94C, followed by 27 cycles of denaturation at 95C for 40 s, annealing at 55C for 40 s, and amplification at 72C for 40 s , followed by a final elongation step of 10 min at 72C.