title: 16S_biome of sea  hair
design: Tufts of seal hair,  trimmed adjacent to the skin, and the gut content of brittle stars were  homogenized in bead-beating tubes at maximum speed for five minutes in  25:24:1 phenol-chloroform-isoamyl (Sigma-Aldrich). DNA was subsequently purified with the  DNeasy PowerMax Soil Extraction kit (Qiagen), according to manufacturers  instruction. DNA from surface sediment  was extracted using the DNeasy PowerMax Soil Exraction kit, according to  manufacturers protocols. Extracted DNA was amplified with the iTru_A_515f and iTru_1_806RB primer set.