title: Illumina HiSeq 4000 paired end sequencing; Comparative analysis of RNA-seq data from human ALT+ cell lines treated with NR2F2 siRNA
design: Comparative analysis of RNA-seq data from human ALT+ cell lines treated with NR2F2 siRNA
library_construction_protocol: RCB0252 cell refer to W-V samples (CLO:0050644). All cell pellets stored at -80C RNA was extracted from fresh frozen cell pellets using a Maxwell semi-automated method with MaxwellÂ® RSC simplyRNA Cells Kit (Promega). RNA purity was assessed using a Nanodrop (OD260/OD280) and an Agilent 2100 Bioanalyser with a Nano-RNA 6000 Chip was used to assess the integrity of the RNA. Illumina truseq non-stranded mRNA protocol, done by Novogene.