title: GSM5369304: Liver-control3; Mus musculus; RNA-Seq
library_construction_protocol: After the total RNA extracted from the samples was treated with DNase I, the eukaryotic mRNA was enriched with Oligo(dT) magnetic beads. Then adding a breaking reagent to break the mRNA into short segments, using the broken mRNA as a template and synthesizing a strand of cDNA by using a random hexamers, then adding a buffer solution, dNTPs, and DNA polymerase I to synthesize the second strand of cDNA, and carrying out PCR amplification enrichment after size selection on the obtained segments after purification and recovery by a kit, repair of a sticky end, the addition of a base' A' at the 3' end and connecting a sequencing joint. The constructed library was qualified by Agilent 2100 Bioanalyzer and ABI Step One Plus Real-time PCR system and sequenced using Illumina sequencing platform. RNA libraries were prepared for sequencing using standard Illumina protocols