title: GSM5369605: RNAseq_Sub12_GF_pear; Homo sapiens; RNA-Seq
library_construction_protocol: Fat biospsies were collected, Stromal-vascular fractions were isolated from scWAT A- and GF-FAT depot, plated and cultured in aMEM suplemented with hEGF and hFGF. The preadipocyte population was enriched by depletion of the stromal-vascular fraction of endothelial cells (CD31 positive cells) using the magnetic beads technology from Stemcell technologies (Cambridge, MA). At confluence, cells were harvested and a fraction of the cells was frozen for RNA extraction. RNA was isolated using RNeasy Mini Kit (Qiagen, Valencia, CA)  Illumina TruSeq RNA Sample Prep Kit  was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using Illumina TruSeq Stranded Total RNA Library preparation kit (Illumina Inc., San Diego, CA) as per the instructions