title: Whole exome sequencing of homo sapiens: bone marrow or peripheral blood from children with acute lymphoblastic leukemia
design: Firstly, genomic DNA was extracted from bone marrow and fragmented, then the end was repaired and  added with "A". Secondly, the DNA fragment was linked with adapter, following with magnetic bead purification and Pre-PCR  was carried out. After another magnetic bead purification, library was hybridized with probe for 16 ~ 24h. Nextly, the probe is bonded to the magnetic bead and nonspecific binding library was rinsed. Finally, post capture PCR amplification was carried out and DNA library quantification was determined by Qubit 3.4 Fluorometer.