title: GSM6255133: Resting microglia - pcHi-C_rep1; Homo sapiens; Hi-C
library_construction_protocol: Cells were harvested, fixed in 1% formaldehyde, snap frozen in dry ice, then stored at -80C. Nuclei extraction was performed prior to further processing. In situ Hi-C was performed using the standard protocol, then 500 ng of the resulting libraries were used to prepare pcHi-C using a custom biotinylated RNA probe set. In In situ Hi-C followed by pcHi-C