title: GSM5946479: KO_treated_PD1_2; Mus musculus; ATAC-seq
library_construction_protocol: Chromatin profiling was performed by ATAC-seq as described in Buenrostro et al. In brief,  50,000 cells per sample were washed in cold PBS and lysed. Transposition reaction was  performed at 37°C for 30 minutes and DNA was purified with MinElute PCR purification kit  (Qiagen). Transposed DNA was amplified for 5 cycles and additional PCR cycles were  evaluated by real time PCR.