title: BisPCR2 of karma-EgDEF1 region in Elaeis guineensis: leaf
design: BisPCR2 (Bernstein et al. 2015, Kamstra et al. 2017) method using starting amount of 2ug genomic DNA and specific primers targeted to NW_011551018.1: 919569-927386 region in E.guineensis genome.