title: GSM5949285: NLP6-1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: For-RNA-seq, whole seedlings from liquid medium or protoplasts were harvested and frozen in liquid nitrogen.  Total RNA was isolated from liquid nitrogen ground samples with the Trizol reagent (Invitrogen), The resulting RNA (0.5 μg) was used for preparing the library For-RNA-seq, NEBNext® Ultra II™ RNA Library Prep Kit for Illumina® using 500 ng of total RNA per library.