title: RNA-Seq of Homo sapiens: adult Male AML, M5
design: RNA was isolated from frozen cells using the Qiagen AllPrep DNA/RNA Mini Kit (QIAGEN). Residual genomic DNA was removed using the RNase-free DNase set (QIAGEN). RNA was quantified using the Nanodrop 2000 spectrophotometer and quality was assessed on a Bioanalyzer using RNA 6000 Nano chips (Agilent Technologies). RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA kit.  Libraries were sequenced on a NovaSeq 6000 instrument (Illumina Inc.) at a target read depth of 25M paired-end reads per sample.