title: GSM5368908: 5-8F-si-NC; Homo sapiens; RNA-Seq
library_construction_protocol: For whole-transcriptome sequencing, total RNA was extracted from tumor specimens by using RNeasy Mini Kit (Qiagen), and 1 μg total RNA was subjected for the depletion of ribosomal RNAs by using Ribo-Zero Magnetic Kit (Illumina), followed by library preparation according to the manufacturer's instructions as in the TruSeq RNA Library  Prep Kit (Illumina). Library was processed with high-throughput sequencing for pair-end of 150 bp, using Hiseq X  Ten sequencer (Illumina, USA). High-quality pair-end reads were mapped using Bowtie 2. After filtering out ribosomal RNAs, reads counts of each gene were then quantitated using HTseq.