title: GSM5767892: 2014-1008_A1_INPUT_Dsim_Zeus; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Chromatin from whole flies (150 flies per technical replicate) was isolated according to standard modENCODE protocols using formaldehyde as the crosslinking agent and sonication. Immunpreciptation was performed using anti-FLAG antibody (Sigma) and protein G beads (GE Healthcare) Libraries for sequencing were prepared using the Ovation Ultra-Low Library System (NuGEN) and sequenced on the Illumina HiSeq platform by IGSB at The University of Chicago