title: GSM6338374: N1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from 293T cells using TRIzol™ (Tiangen Biotech Co., Ltd.) reagent, and RNA purity was checked using an Implen NanoPhotometer® spectrophotometer (Implen USA, Inc.). RNA integrity was assessed using the RNA Nano6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Inc.). A total of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep kit for Illumina® (New England BioLabs, Inc.) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample. The library preparations were sequenced on a NovaSeq 6000 platform (Illumina, Inc.), and 125 bp/150 bp paired-end reads were generated.