title: GSM5369116: NOD-AI4-2; Mus musculus; RNA-Seq
library_construction_protocol: Thymi were dissected, mashed and passed through nytex to create single cell suspensions.  Live gated CD4+CD8+ cells were sorted by flow cytometry and collected into FBS.  Cell pellets were resuspended in TRIzol and frozen.  Total RNA was extracted according to Thermo Fisher Scientific's TRIzol  protocol and  Qiagen RNeasy MiniElute Cleanup to RIN >9. KAPA stranded RNA libraries