title: Illumina HiSeq 2500 paired end sequencing; Raw reads: Bstacei
design: Library preparation was carried out using TruSeq Stranded mRNA Library Prep Kit (Illumina, Inc.), generating Paired-End (PE-125 bp) libraries with insert size of 300 to 600 bp. Adapters and low quality reads were removed and filtered with Trimmomatic-0.32 (ILLUMINACLIP:$TRIMMOMATIC/adapters/TruSeq3-PE-2.fa:2:30:10 CROP:120 HEADCROP:13 SLIDINGWINDOW:15:28 MINLEN:25)