title: 16s rRNA  amplicon  sequence of permafrost soil
design: gDNA was extracted from soil sample using MP Bio kit then cleaning and concentration of gDNA was done followed by PCR amplification by universal primer for 16s v3-v4 region. Quality and quantity assessment by Qubit Flurometer and Agarose Gel Electrophoresis. Multiplex amplicon lib were prepared on Illumina MiSeq and raw reads were obtained.