title: total RNA: total RNA
design: pBADHisA plasmid with the cloned protein GsSir2/Ago from Geobacter sulfurreducens PCA (NP_952414.1) expressed in E.coli DH10B cells together with pCDF-Kn plasmid. A total RNA from induced cells was extracted using SPLIT RNA Extraction Kit (Lexogen cat#008). Then rRNA was removed using RiboCop for Gram negative Bacteria (Lexogen cat#126). DNA library of a total RNA was prepared using CORALL Total RNA-Seq Library Prep Kit (Lexogen cat#095).