title: 16S amplicons reads of microbiomes from thrips affecting avocado crops in neotropical Northwestern Range of the Colombian Andes
design: Total metagenomic DNA was extracted from pools of thrips specimens of the same morphotypes (n = 10) obtained from the same avocado crop culturing farm using Total DNA was extracted using chemical lysis with  50mM Tris-HC1 buffer (pH 8.5), 1mM EDTA, 0.5% SDS , 200 g/ml and proteinase K followed by purification with  FastDNA SPIN KIT (MP Biomedicals). DNA was quantified using Nanodrop 2000 (Thermo Fisher Scientific, USA). PCR amplification was performed using primers 515F (5'-GTGYCAGCMGCCGCGGTAA-3') [47] and 806R (5'-GGACTACNVGGGTWTCTAAT-3') [48] targeting the hypervariable region V4-V5 of 16S rRNA genes. The PCR products were used for Nextera TX library construction and were sequenced using Illumina MiSeq platform 2  250 bp.