title: Illumina HiSeq 3000 paired end sequencing; Raw reads: DE01087_REF_RNAseq_flowers
design: RNA was extracted from five different tissues (leaf, flower, anther, pollen, root) following the RNA extraction protocol of Yaffe et al. (2012). The remaining DNA was removed with DnaseI (EN0521, Thermo Scientific) following manufacturerâ€™s recommendations. The quality was checked with an RNA 6000 Nano Chip on an Agilent Bioanalyzer 2100 (Kit 5067-1511, Agilent Technologies). All RIN-scores were higher than 5.4. For the library preparation, the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina in combination with the Poly(A) mRNA Magnetic Isolation Module (E7760, E7490, NEB) was used. The heat fragmentation was performed for a duration of 9 min resulting in final library sizes of around 545 bp. All 5 libraries were equally pooled and sequenced on one lane of an Illumina HiSeq 3000 system in paired-end mode and 150 bp read-length.