title: GSM6214842: Sample_5_NRF2_CDDO-Me 2; Homo sapiens; RNA-Seq
library_construction_protocol: IMR90 iPSCs were induced for cardiac differentiation using a growth factor-guided protocol. Cardiac spheres were generated from cells at differentiation day 5 and subjected to NRF2 activator CDDO-Me or DMSO treatment from day 14 to day 24. RNA samples from cells at differentiation day 24 or LV samples were prepared using Bio-Rad RNA extraction kit and 1 µg of total RNA/sample was used for the construction of sequencing libraries using Illumina TruSeq RNA Sample Prep Kit. Libraries were generated using the Illumina standard TruSeq protocol and next-generation sequencing was done through an Illumina HiSeq 2500, 75bp paired end sequencing.