title: 16SrRNa gut microbiota: adult dog
design: Metabarcoding analyses using sequencing of amplicon were investigated throughout the hypervariable genomic region (V3-V4 region 16SrRNA gene amplification), using an NGS approach on Illumina Platform. From 180-220 mg of each fecal sample the total amount of DNA was extracted. PCR was firstly used to amplify template from a DNA sample using specific denatured primers targeting the 16S V3 and V4 region. Then a second PCR was perform to attach dual index and Illumina sequencing adapters using the Nextera XT Index kit. The final library was diluted 1:50 and then pooled. Finally, the pooled library was sequenced using the PhiX library as control.