title: PacBio Sequencing of HSV-2 strain G genomic DNA
design: 1,000 ng of viral genomic DNA was sheared to 10-12 kb using the Megaruptor 3 (Dia-genode, Inc.). 482 ng of sheared DNA was used to prepare a SMRTbell template library with the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA) following the microbial library protocol (Procedure & Checklist  Preparing Multiplexed Microbial Li-braries Using SMRTbell Express Template Prep Kit 2.0). Fragments shorter than 3 kb were removed from the library using a bead size selection method. Sequencing primer v4 was annealed and Sequel II polymerase 2.0 was bound to the library prior to loading on one SMRT Cell 8M on the Sequel II System using diffusion loading. Sequencing was per-formed with 2h pre-extension and a 30h movie.