title: SHAPE-MaP of Homo sapiens: Replicate 1 of amplicon 2 miR-17-92 Wild type + Srsf3 siRNA (Srsf3 knockdown) plus-reagent
design: HEK293 cells were transfected with Silencer Select pre-designed siRNA for SRSF3 or negative control (Thermo Fisher Scientific) according to the manufacturer's instructions. The cells were also transfected with miR-17-92 constructs. In-Cell SHAPE-MaP was performed in control and SRSF3 knockdown cells. 2-methylnicotinic acid imidazolide (NAI, 100µM) was used as the electrophile (+ reagent) and DMSO as a control (- reagent). NAI was quenched with DDT to achieve accurate RNA structure probing in the optimal reaction time frame. Sequencing was performed using Illumina MiSeq.