title: WGS of SARS-CoV-2: Mumbai
design: cDNA from viral RNA of SARS-CoV-2 was subjected to targeted amplification by PCR. Sequencing was performed on MinION MK1B using SpotON flow cell (FLO-MIN106) of Oxford Nanopore Technology. Amplified DNA were subjected to barcoding and adapter ligation using either the rapid barcoding MIDNIGHT protocol or native barcoding ARTIC protocol. Base-calling and de-multiplexing of Nanopore raw data were performed by Guppy (v.5.0.17) tool.