title: GSM5989989: HUVEC LINC00607 CRISPR/Cas9 knock-out (KO) 3; Homo sapiens; ATAC-seq
library_construction_protocol: 50.000 HUVEC were used for ATAC library preparation using llumina Tagment DNA Enzyme and Buffer Kit (Illumina). The cell pellet was resuspended in 50 µL of the lysis/transposition reaction mix (25 µL TD-Buffer, 2.5 µL Nextera Tn5 Transposase, 0.5 µL 10% NP-40 and 32 µL H2O) and incubated at 37°C for 30 min followed by immediate purification of DNA fragments with the MinElute PCR Purification Kit (Qiagen). Purification of DNA fragments was done with the MinElute PCR Purification Kit (Qiagen). Amplification of Library and Indexing was performed as descried elsewhere (Buenrostro JD, Wu B, Chang HY, Greenleaf WJ. ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol. 2015).