title: Illumina HiSeq 2000 paired end sequencing; RNA-seq of iron-rich pelagic aggregates (iron snow) in 2017 and 2018
design: RNA-seq of iron-rich pelagic aggregates (iron snow) in 2017 and 2018
library_construction_protocol: We conducted sampling twice (2017, 2018). Iron-rich pelagic aggregates (iron snow) were collected directly onto silicate glass filters using an electronic water pump installed below the redoxcline.   Glass filters with iron snow were cut into smaller pieces and oxalate extraction buffer was added to remove iroN(III). Following that, NaPO4 buffer and TNS solution was used to lyse cells. RNA was extract using phenol-chloroform-isoamyl alcohol and chloroform-isoamyl alcohol. Total RNA was subjected to library preparation using the NEBNext Ultra II directional RNA according to the manufacturerâ€™s instructions. Size selection was adapted to select for fragments ranging between 150-200 bp in length.