title: GSM5239848: condKO_rep1_time0; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with the GeneMatrix universal RNA purification kit (Roboklon). 2 µl of 1:100 diluted ERCC RNA Spike-In Mix (Thermo Scientific) was added per µg total RNA. After depletion of rRNA using the Ribo-Zero Gold Kit (Illumina), sequencing libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and sequenced on a NextSeq550 system (Illumina) with 75 bases single end.