title: GSM5596120: UK5099-treated replicate 2; Mus musculus; ATAC-seq
library_construction_protocol: 50 000 T cells were washed 1x with PBS and prepared for transposase treatment T cell pellet was resuspended in 50 microliter of ice-cold lysis buffer (10mM Tris-Cl (pH 7.4), 10mM NaCl, 3mM MgCl2 and 0.1% (v/v) of NP-40. Cells were centrifuged immediately and the resulting pellet (nuclei) was resuspended in 50 microliter of transposase reaction mix  (Illumina), followed by incubation at 37°C for 30min. Tagmented DNA was cleaned using QIAGEN MinElute PCR Purification kit as described in the kit's protocol. Library preparation was performed using Indexes (R#20027213) and NEBNext High-Fidelity 2X PCR Master Mix(M0541), using the following program: 5 min 72°C, 30 s 98°C; 10 cycles: 10 s 98°C, 30 s 63°C, 1 min 72C°. The libraries were then cleaned using Agencourt AMPure XP magnetic beads (A63880, Beckman).