Havinthus rufovarius venom gland transcriptome
A venom gland transcriptome was generated from two adult (one male and one female) H. rufovarius. The entire venom gland complex (including anterior and posterior main glands and accessory gland) was removed by dissection and stored in RNAlater (Ambion). Total RNA was extracted using a DNeasy kit, and the mRNA isolated using Dynabeads mRNA Direct kit. The mRNA was sequenced with the Illumina NextSeq instrument at the IMB Sequencing Facility. The transcripts were then assembled using Trinity using default trimming parameters