GSM6257341: IL-3-simulation_03; Mus musculus; RNA-Seq
Bone marrow-derived basophils were stimulated with 10 ng/mL IL-3 or control PBS for 6 hours and subjected to RNA extraction by using Nucleospin RNA Plus XS (Macherey Nagel) Library construction for bulk-RNA sequencing was conducted according to the previous study (Ogawa et al. Biochem Biophys Res Commun. 2022). Briefly, 100ng of amplified cDNA libraries were subjected to Illumina library construction by using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs). Fragmentation, end-repair, dA-tailing, adaptor ligation, and barcoding PCR were sequentially conducted according to the manufacturer's instruction.