GSM4759332: BIBB-515 sample 9; Mus musculus; RNA-Seq
Flash frozen hepatic samples were homogenized in Qiazol (QIAGEN) and RNA isolated according to the manufacturer's protocol with RNeasy columns (QIAGEN). Isolated RNA was converted to cDNA using a High Capacity cDNA Synthesis Kit (ThermoFisher) according to the manufacturer's instructions. Primers targeted at genes of interest were designed and reactions were run in triplicate using KAPA SYBR FAST qPCR Master Mix (Roche) with 8 ng of cDNA per reaction.
GEO Accession
GSM4759332