GSM5370641: 218_D1_control; Mesocricetus auratus; RNA-Seq
Frozen tissues were pulverized in 1 mL of Trizol (Thermofisher Scientific, Waltham, MA), 200 µL of 1-Bromo-3-chloropropane (MilliporeSigma, St. Louis, MO) was added, samples mixed, and centrifuged at 16,000 x g for 15 min at 4°C.  RNA containing aqueous phase of 600 µL was collected from each sample and passed through Qiashredder column (Qiagen, Valencia, CA) at 21,000 x g for 2 minutes to homogenize any remaining genomic DNA in the aqueous phase. Aqueous phase was combined with 600uL of RLT lysis buffer (Qiagen, Valencia, CA) with 1% beta mercaptoethanol (MilliporeSigma, St. Louis, MO) and RNA was extracted using Qiagen AllPrep DNA/RNA 96-well system (Valencia, CA). An additional on-column DNase 1 treatment was performed during RNA extraction. Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus workflow (Illumina, San Diego, CA).
GEO Accession
GSM5370641