RNAseq of Hordeum vulgare subsp. vulgare Bowman mock-inoculated with oil, 2 dpi, replicate 3
One set of the first-leaf-stage seedlings of cv. Bowman and the BW746 was inoculated with P. hordei pathotype 5453 P+ and one set was used for mock inoculation. The first leaves from the inoculated plants were harvested at 2 dpi, flash-frozen in liquid nitrogen until RNA extraction. Twelve samples (two genotypes, two treatments/genotype, and three biological replications/treatment) were subjected for total RNA extraction using the SpectrumTM Plant Total RNA kit (Sigma-Aldrich) following the instructions from the manufacturer. The RNA samples were initially quantified using a NanoDropTM 1000 Spectrophotometer (Thermo Fisher Scientific Ltd), the degradation and potential contamination were checked on agarose gel 1.5%, and the RNA integrity and quantitation were measured using Agilent 2100. The library was prepared using the NEBNext UltraTM RNA Library Prep Kit.