GSM5242741: TBPE-3; Homo sapiens; miRNA-Seq
TBPE or TPE specimens were centrifuged at 300 g for 10 min at 4°C. The supernatant was taken and centrifuged at 10,000 g for 30 min at 4°C. Then the supernatant was ultracentrifuged at 100,000 g for 70 min at 4°C. The pellet was resuspended in PBS buffer and ultracentrifuged again at 100,000 g for 70 min. After ultracentrifugation, the pellet was suspended with 50 μl PBS buffer and saved at -80°C. Libraries were prepared according to Small RNA Sample Pre Kit. Briefly, The special structure of the 3 'and 5' ends of Small RNA (the 5 'end has complete phosphate group and the 3' end has hydroxyl group) was used, and total RNA was used as the starting sample cDNA was synthesized by reverse transcription of Small RNA by adding connectors to both ends of the RNA.
GEO Accession
GSM5242741