GSM5903722: shoot-N_mRNA, Rep2; Solanum lycopersicum; RNA-Seq
Tomato root and shoot tissue was ground in liquid nitrogen and total RNA was extracted using mirVana kit following the manufacturer's protocol for total RNA extraction. Turbo DNase was used to remove contaminating DNA and then the quality of extract total RNA was analyzed using Bioanalyzer in Purdue Genomics Core. Poly(A) RNA was prepared for sequencing using standard Illumina protocols