GSM5903216: ATAC-seq ICSI 2-cell stage embryos rep2; Mus musculus; ATAC-seq
Omni-ATAC seq was performed by following the methods as reported in the previous study (Corres et al., Nature Methods 2017). For Omni ATAC-seq, DNA was isolated by MinElute PCR purification (Qiagen) after ATAC-reaction for 30 min at 37˚C . Twenty-two ul of eluted sample were stored  at -20˚C until subsequent PCR amplification. After 5 cycles pre-amplification, additional 9 cycles of  PCR were performed. Amplified DNA was purified by MinElute PCR purification.