GSM4972186: ExoSeq_KCl_Rep2; Mus musculus; RNA-Seq
Total RNA was extracted as described previously and depleted from rRNA and SRP-RNA The 5' ExoSeq library construction was performed as described (Afik et al., 2017), with the following alterations. Instead of poly(A)-selected RNA we started with a rRNA and signal recognition particle (SRP)-RNA depleted RNA sample. rRNA & SRP-RNA was depleted using a commercially available kit (RiboCop rRNA depletion kit V1.2 (human/mouse/rat), Lexogen) according to the manufacturer's instructions with some modifications. For one 5' ExoSeq library sample, RNA from three depletion reactions (3x 500 ng total RNA) was pooled before library construction.
GEO Accession
GSM4972186