GSM5243261: PBMCs from Healthy2_Vehicle non-hashed – Post discovery cohort (scRNA-seq); Homo sapiens; RNA-Seq
As described in the v3.0 single-cell 3' mRNA kit (10x Genomics) protocol (CGO00183 Rev C), after GEMs generation and reverse transcription, the cDNA was extracted by Dynabeads MyOne SILANE clean up following manufacturer's instructions. The obtained cDNA was then used for amplification. The amplified cDNA was subjected to enzymatic fragmentation, end repair and A-tailing. Size selected fragments subsequently underwent R2 adaptor ligation and Index PCR for the insertion of sample index for library demultiplexing, P5 and P7 Illumina adapters. Libraries were purified by SPRI selected beads clean up. Fragments size distribution and concentration were verified on an Agilent Bioanalyzer High Sensitivity Chip (Agilent Technologies). Libraries were sequenced on a NovaSeq6000 (Illumina) to obtain approximately 25000 reads per cell.
GEO Accession
GSM5243261