GSM4974123: RM01_CP_CTCF; Macaca mulatta; ChIP-Seq
Cells were fixed by final concentration of 1% formaldehyde (Sigma-Aldrich, F1635), mixed for 10 mins at room temperature. Fixation was quenched using 2.5 M glycine at room temperature for 5 mins and immediately centrifuged at 800g for 10 mins. Supernatant was removed and frozen in liquid nitrogen and stored at −80°C until further use. Five million fixed cells were lysed for 10min, and then sonicated for 20 cycles using Bioruptor (Diagenode). After sonication, chromatin was precleared using Protein A dynabeads. CTCF primary antibody-beads added to the precleared chromatin and incubated overnight at 4°C with rotation. Beads were then washed, extract the DNA, end repair, add A, add adaptor reaction and PCR amplification, DNA products size selection were performed step by step.
GEO Accession
GSM4974123