GSM6256906: Hippocampus, 7-month-old 5×FAD mice, rep1 [5H_1]; Mus musculus; ncRNA-Seq
Total RNA was isolated from the cerebral cortex and hippocampus using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. To construct the lncRNA library, sequencing of paired ends mode was used. rRNA was removed from the total RNA of all tissue samples using the Ribo-off rRNA Depletion kit (Vazyme Biotech Co., Ltd) based on the enzymatic reaction between the DNA probe and RNaseH. The VAHTSTM Stranded mRNA-seq Library Prep Kit by Illumina® (Vazyme Biotech Co., Ltd) was employed for fragmentation, cDNA synthesis, cDNA fragment modification, magnetic bead purification and fragmentation sorting, and library amplification according to the manufacturer's recommendations. After quality control and purification, the lncRNA library was obtained to meet the requirements of Illumina platform sequencing.