GSM5709955: DM-P; Rattus norvegicus; RNA-Seq
Buccal and lingual gingiva tissue around the maxillary left second molars were removed minced on ice to less than 1 mm cubic pieces. They were digested by collagenase I (2mg/mL, GIBCO), collagenase II (2 mg/mL, GIBCO) and trypsin-EDTA (0.25%, GIBCO) at 37℃ for 60 min. Cell suspension was then filtered through a 40-μm strainer (ThermoFisher Scientific), and the supernatant was removed after centrifugation. RNA libraries were prepared with Chromium Single Cell 3' Reagent v3 Kits according to to the manufacture's protocol.
GEO Accession
GSM5709955