Illumina NovaSeq 6000 sequencing; Clonal Hematopoiesis–Driver DNMT3A Mutations and Immune Cells in Heart Failure
Clonal Hematopoiesis–Driver DNMT3A Mutations and Immune Cells in Heart Failure
Blood was obtained from patients with chronic HF at approximately the same time (between 10:00 am and 12:00 pm) on study days. Informed consent was obtained from all patients. The study was approved by the local ethics review board and complies with the Declaration of Helsinki.  Blood was collected in sodium citratecontaining cell preparation tubes (CPT Vacutainer; Becton Dickinson) which were centrifuged at 1500g for 20 minutes at room temperature. The upper layer containing mononuclear cells and plasma was collected and cells were washed and then resuspended in PBS and used for droplet scRNA-seq. Standard scRNA-seq 10x Genomics protocol as described below. https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v31-chemistry-dual-index Standard scRNA-seq 10x Genomics protocol as described below. https://support.10xgenomics.com/single-cell-gene-expression/library-prep/doc/user-guide-chromium-single-cell-3-reagent-kits-user-guide-v31-chemistry-dual-index
Experimental Factor: genetic modification
none