ATAC-seq of mandarin fish (Siniperca chuatsi): adult liver
Liver from adult mandarin fish was used for construction of ATAC-seq library. Tissue was washed with 0.09 % NaCl solution and grinded in liquid nitrogen, then followed by incubation with lysis buffer for 10 min. The cell suspension was filtered through a 41-m cell strainer and then washed three times with cold PBS buffer. After confirming the purity and intactness of cell nuclei, library construction was carried out. The library was sequenced on the Illumina HiSeq X platform. Adapter sequences and low-quality reads were removed by Cutadapt.