RAD-seq of Primulina danxiaensis
Genomic DNA was normalized to a concentration of 50 ng/l, digested with restriction endonuclease EcoRI and MseI, ligated to sequencing adaptors and individual barcodes, and amplified by polymerase chain reaction (PCR). Then 16-24 individuals were pulled together and processed into multiplexed RAD libraries following established methods, and finally sequenced on an Illumina HiSeq 2000 platform.