RNAseq of mus musculus ventricular mononuclear remote cardiomyocytes 911
RNA from CMs or nuclei was extracted using the RNeasy Micro Kit including on-column DNAse digestion. RNA from CMs was reverse transcribed and amplified with the Nugen Ovation RNA-Seq System V2 (Nugen). The resulting cDNA was fragmented (Bioruptor, Diagenode) and 100 ng fragmented cDNA was used to construct sequencing libraries using the NEBNext Ultra DNA Library Prep Kit (NEB).