RNA-Seq of Hordeum hexastichum infected with BYDV PAS and PAV (final virus populations)
A ribo-depletion procedure was first carried out using the RiboMinus Plant Kit for RNA-Seq (Thermo Fisher Scientific). Then, a total RNA library was constructed with the TruSeq Stranded mRNA kit (Illumina). Finally, samples were sequenced using the Illumina NextSeq 500 system (2150 nucleotides).