GSM5866861: H9 naïve untreated; Homo sapiens; RNA-Seq
Samples EZH2i d2, d4 and untreated control naïve as well as EZH2i d7 and untreated control primed cells were multiplexed following the manufacturer's protocol. In brief, cells were dissociated, treated with mouse feeder removal beads (naïve cells), counted and resuspended in Cell Multiplexing Oligo. After oligo incubation, cells were washed for a total of three times using PBS-/- containing 1% BSA (Sigma Aldrich; A7284) and counted using the NucleoCounter® NC-200™ automated cell counter. At this point, cells were pooled accordingly: EZH2i d2, d4 and WT naïve cells, and EZH2i d7 and WT primed cells, respectively, and each pool was counted once more.The EZH2i d7 naïve cell sample was collected following the dissociation protocol outlined above. Again, mouse feeder removal beads were used to reduce the amount of MEFs going through into the final cell product. naïve cells were counted and diluted to 1 x 10^6 cells/ml.