PacBio WGS sequencing of TDa95/00328 (Dioscorea alata L.)
HMW gDNA was extracted from fresh leaves using a CTAB protocol from TDa95/00328 plants sourced from IITA Ibadan, Nigeria and grown at ICRAF in Nairobi, Kenya. Pacific Biosciences (PacBio) library preparation and sequencing were performed at the UC Davis Genome and Biomedical Sciences Facility. Fragments larger than 15kb were sequenced on a PacBio Sequel machines with v2 chemistry as continuous long reads.