GSM5243453: DMSO Input (biological replicate 2); Homo sapiens; ChIP-Seq
Cells were fixed in 1% formaldehyde (final concentration) for 10 minutes; cells were lysed, and chromatin was sonicated to an average 200 bp. Immunoprecipitation was performed using H3K27ac (Cell Signaling Technologies, 8173) and normal rabbit IgG (Bethyl Laboraties, P120-101). Input samples (1%) were also saved for library production. Immunoprecipitated complexes were washed, eluted from beads, treated with Proteinase K and crosslinks were reversed. DNA was purified using a PCR Cleanup Kit (Qiagen, 28104). Two independent experiments (n=2) were used for library production and sequencing. DNA libraries were produced according to manufacturer instructions using the NebNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Multiplex Oligos for Illumina (Index Primers Set 1 and Set 2) from New England Biolabs were used as index primers during library preparation.
GEO Accession
GSM5243453