GSM6215032: dyrk1a_HOM3; Danio rerio; RNA-Seq
Total RNA was extracted from isolated larval heads with the eyes removed (1 head per sample) using the RNeasy Micro Kit (Qiagen) or the Invitrogen RecoverAll  RNA Isolation Kit (Invitrogen). To control for background variation, RNA was extracted from sibling- or cousin-matched larvae from heterozygous incrosses of scn1labΔ44/+, chd8Δ5/+ and dyrk1aaΔ77/+:dyrk1abΔ8/+ fish RNA library preparation was performed using NEBNext Single Cell Low Input RNA Library Prep Kit (Cat# E6420L) Sequencing was done at 25 million reads/sample using NovaSeq 6000 (Illumina) (Yale Center for Genome Analysis)