GSM5243409: WFMD132A - Case; Homo sapiens; OTHER
Genomic DNA was extracted from the endometrial biopsies using the RNeasy Mini Kit (Qiagen, Hilden, Germany), omitting the DNase treatment, and following the manufacturer's protocol. The quantity and purity of nucleic acids was assessed using the Nanodrop 2000. In order to retain bacterial DNA and eliminate the RNA that might inhibit the PCR reaction, RNase A (Qiagen Hilden, Germany) was added to the first PCR mastermix. The hypervariable V3 region of the 16S rRNA gene was amplified by using a two-stage (nested) PCR approach. sl1p pipeline (Whenlan and Surette, 2017, BMC Microbiome) 16S rRNA gene sequencing
GEO Accession
GSM5243409