Whole-genome resequencing of 2AF247
For each rapeseed accession, young leaves were collected from a single plant for genomic DNA extraction.A total amount of 1.5g genomic DNA was used for the DNA sample preparations. Qualified DNA was cut into 350-bp fragments randomly using hydrodynamic shearing system. Ended repair, added A-tail, ligation of paired-end adaptors, purification and PCR amplification with 350-bp inserts were sequentially processed to constructed a paired-end library and then were sequencing on the IlluminaHiSeq 4000 platform.