metagenome sequencing of oral samples:  S103
Saliva was processed according to OmniGene instructions post collection. DNA was isolated using the Qiagen QIAAmp DNA isolation kit with some modifications. A bead beating step was incorporated. SK38 microbiome beads (Precellys) were added to 400uL of saliva and homoogenised at 4500RPM in 3 x 30 second cycles (Precellys homogeniser) . 200 uL of the homogenised saliva was then used in the DNA isolation step. A mock isolation (no saliva) was completed in parallel. Tissue DNA was isolated from tissue using the QIagen Fast Prep DNA isolation kit with some modifications. Tissue was homogenised using the bead provided with the kit using a vortexer (Vortex Genie). The bead was then removed from the homogenised tissue and SK38 microbiome beads (Precellys) were added and homoogenised at 4500RPM in 3 x 30 second cycles (Precellys homogeniser). The tissue / bead mixture was centrifuged briefly to collect beads and the supernatant was used to continue with the DNA isolation in accordance with the manufacturers instructions. A mock isolation (no tissue) was completed in parallel. Sequencing was done at ACE laboratory with primer pair 341-806.