RNA-Seq of Siphonotidae
Total RNA was isolated using a Qiagen RNA Extraction kit according to the manufacturer's instructions (Qiagen, Hilden, Ger- many). Quality of mRNA was assessed using a NanoDrop ND2000c UV spectrophotometer (Thermo Fisher Scien- tific, Wilmington, MA, USA). cDNA library preparation and transcriptome sequencing were conducted by Allwe- gene Technology Co., Ltd., Beijing, China. Briefly, cDNA libraries were constructed with the TruSeq RNA Library Prep Kit v2 (Illumina, CA, USA) following the manufac- turers instructions.