Illumina HiSeq 4000 sequencing; Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.
Cross-species screening platforms identify EPS-8 as a critical link for mitochondrial stress and actin stabilization.
Cell line H9 NPCs were plated in a medium containing rock inhibitor. The following day, the medium was replaced with medium lacking rock inhibitor. Following an additional day of growth, cells were treated with 10 µM antimycin or equal volume ethanol as vehicle control.  Medium was aspirated and cells were collected in trizol (Thermo Scientific, 15596018) after 8 hours, mixed with equal volume of isopropanol, and RNA was extracted using RNeasy Mini Kit (Qiagen 74106). RNA concentration from 3 biological replicates was measured using an Agilent Bioanalyzer at UC Berkeley Functional Genomics Core Lab (FGL). Total RNA (500 ng) was used for library construction using the mRNA HyperPrep Kit (KAPA Biosystems KR1352). Compatible dual‐indexed “barcode” adaptors (KAPA KR1318) were chosen so that all 10 libraries could be multiplexed on one lane on the Illumina HS4000 sequencer.  RNA concentration from 3 biological replicates was measured using an Agilent Bioanalyzer at UC Berkeley Functional Genomics Core Lab (FGL). Total RNA (500 ng) was used for library construction using the mRNA HyperPrep Kit (KAPA Biosystems KR1352).
Experimental Factor: organism
Homo sapiens
Experimental Factor: genetic modification
none
Experimental Factor: compound
antimycin
Experimental Factor: dose
10
Experimental Factor: time
8