Illumina mate-pair sequencing of Amoeboaphelidium protococcarum strain X5
Amoeboaphelidium protococcarum was collected from plaques in the inoculated algal culture of Scenedesmus obliquus, grown on a solid mineral medium. The DNA was extracted using Diatom DNA Prep kit (Isogen Laboratory) after lysing the cells in the RLT buffer (RNeasy kit, Qiagen) and incubating the lysate with silica-gel granules in solution with 1/3 volume of ethanol. The mate-pair library was prepared following the Nextera library preparation protocol with an estimated mean fragment length of 6950 bp.