RNA-seq of Datura stramonium
Total RNA was extracted from two leaves. Collected leaves were flash-frozen and stored at -80C. RNA was extracted using TRIZOL extraction method. RNA quality and quantity were determined using a Nanodrop 2000 instrument (Thermo Scientific) and Bioanalyzer Chip RNA 7500 series II (Aglient). Libraries were prepared using total RNA (fragment size of 500 pb). Samples were sequenced using Illumina NextSeq 500 under a paired-end 2 x 75 mode.