human saliva metagenome
gDNA extrated using commercial kit (PowerSoil DNA Isolation Kit, MO BIO laboratories, Inc. Carlsbad, CA) and stored in -80C. Our library preparation protocol consisted of two main amplification steps, one to amplify and tag 16S rRNA variable region V3-V4 (primers 341F and 805R), and a second to add final Illumina adapters.