Tnseq of Pveronii
Customized NEBNext UltraTM II FS CBA Library Prep Kit for Illumina, including DNA fragmentation, end-repair, ligation to NEBNext loop adaptors  and USER enzyme treatment; Tn-end fragment enrichment by two PCR (21 cycles), amplifying the left-end transposon end (TnL) and one 24 indexed P7 primers  for Illumina (NEBNext Multiplex Oligos for Illumina; index primers Set 1 and Set 2 ; SPRI-bead purification; then final nested PCR with 6 cycles, and one of four TnL-anchored primers containing the P5-adapter sequence for Illumina and a phasing box, in combination with the TruSeqP7_Fw primer. Sequencing using the MiniSeq System Mid-Output Kit 150 cycle with custom primers (TruSeq Read1 and TruSeq_IndexRead1).