Metabarcoding of mangrove: collected from mud sediments
DNA purification from samples were performed with Quick-DNA Fecal/Soil Microbe Miniprep (Zymo), according to manufacturer instructions. The quality and quantity of the extracted DNA was evaluated by spectrophotometry using Nanodrop 2000 (Thermo). Twenty nanograms of DNA was utilized as a template for 18 cycles of amplification of the V4 region of the 16SrRNA gene, using the primers 515F and 806R (Caporaso et al. 2011) and GoTaq Master Mix (Promega). The PCR products were quantified using the Qubit dsDNA HS kit (Invitrogen) and sequenced using the 300V2 Sequencing Kit (Illumina) on an Illumina MiSeq (Illumina).