GSM6257331: TSLP-elicited mature BMBA_02; Mus musculus; RNA-Seq
FcεRIαhiCD49blo immature and FcεRIαloCD49bhi mature basophils isolated from either IL-3- or TSLP-elicited BMBAs by FACS sorting. Total RNA was extracted from isolated cells by using Nucleospin RNA Plus XS Kit (Macherey-Nagel) Library construction for bulk-RNA sequencing was conducted as described the previous study (Shichino et al. JCI Insight 2019). Sequencing was performed by using an Ion Hi-Q Chef kit, an Ion PI v3 Chip kit, and an Ion Proton Sequencer (Thermo Fisher Scientific) according to the manufacturer's instructions.