Berberine2
Metagenomic sequencing analysis was performed. Briefly, 12 metagenomic DNA libraries were constructed using NEBNext LtraTM DNA Library Prep Kit (NEB, USA) for Illumina. PCR was used to amplify 300400-bp-long DNA fragments. Metagenomic sequencing was carried out on an Illumina Novaseq 6000 platform at Gene Denovo Biotechnology Co., Ltd. (Guangzhou, China). Clean data was obtained from raw data using FASTP 18.0 software (Chen et al., 2018), which was used for further genome assembly.