amplicon sequences of 52 wild strains
Eight genomic loci harboring wtf genes were selected for amplicon sequencing according to the protocol at https://www.pacb.com/wp-content/uploads/Procedure-Checklist-Preparing-SMRTbell-Libraries-using-PacBio-Barcoded-M13-Primers-for-Multiplex-SMRT-Sequencing.pdf.