GSM5965477: iko-c-t4; Mus musculus; RNA-Seq
The colonic muscularis were isolated by manually, and flash frozen on liquid nitrogen. Total RNA was harvested using TRIZOL reagent. Single nuclei were extracted following the protocol provided by 10X Genomics (CG000375). For bulk RNA-seq, RNA libraries were prepared for sequencing using standard Illumina protocols. For snRNA-seq, nuclei RNA libraries were processed according to the 10X Genomic single cell 3' protocol. Completed 10X libraries were sequenced using standard Illumina protocols.