GSM5965533: MYCN-overexpressing cell line in long-term culture replicate 3 [MYCN.2828]; Gallus gallus; RNA-Seq
The central region of E14 chicken retina was dissected and dissociated with 1X Trypsin EDTA. The total RNA was extracted with Qiagen Rneasy Micro Kit (Qiagen, #74004). The RIN value and RNA concentration was measured by TapeStation and 1µg of total RNA was sent for library construction. MYCN-overexpressing cells were taken and dissociated by vibration and pipetting. The total RNA was extracted with Qiagen Rneasy Micro Kit (Qiagen, #74004). The RIN value and RNA concentration was measured by TapeStation and 1µg of total RNA was sent for library construction. The library was prepared using the TruSeq stranded mRNA library preparation kit with polyA selection (Illumina Inc.).