RNAseq of Danio rerio Bdnf mutant
Indexed libraries were prepared from 1 g/ea purified RNA with TruSeq SmallRNA Sample Prep Kit (Illumina) and TruSeq Stranded Total RNA Library Prep Kit (Illumina). Libraries were quantified using the Agilent 2100 Bioanalyzer and pooled such that each index-tagged sample was present in equimolar amounts, with final concentration of the pooled samples of 2 nM. The pooled samples were subject to cluster generation and sequencing using an Illumina HiSeq 2500 System (Illumina) in a 1x50 single read (SmallRNA) and 2x100 paired-end (RNA-Seq) format at a final concentration of 10 pmol.