GSM6290607: NO3_WT rep2; Actinobacillus pleuropneumoniae serovar 1 str. 4074; RNA-Seq
Total RNA was isolated from strains WT, ΔnarQ, and ΔnarP cultured to mid-log phase supplemented with or without NaNO3 (5 mM) in anaerobic conditions. Total RNA was extracted using Trizol lysis according to the protocol recommended by the manufacturer (Total RNA Extraction Kit, Tianmo Biotech, Beijing, China), with three replicates per condition group. RNA libraries for RNA-seq were prepared using IlluminaTruSeq Stranded mRNA LT Sample Prep Kit following manufacturer's protocols.