GSM5866633: ImPACCT-01-006-II-2-RNA; Homo sapiens; RNA-Seq
In brief, biopsies were homogenized using the tissue homogenizing CKMix ceramic beads (Precellys, P000918-LYSK0-A) and subsequently processed for RNA isolation using the column-based RNA isolation (RNeasy Kit, Qiagen). RNA yield and quality were evaluated using spectrometry (Nanodrop 2000/200c, Thermo Fisher) and the BioAnalyzer (2100 Bioanalyzer, Agilent), respectively. RNA libraries were prepared for sequencing using standard Illumina protocols