16s rRNA sequencing of mus musculus: adult feces
Wild-type (WT) C57BL/6NHsd mice were obtained from ENVIGO (Indianapolis, IN). Cgastm1a(EUCOMM)Hmgu mice (Cgas-/- or cGAS KO mice) were kindly provided by Dr. Charlie Rice (Rockefeller University, NY). The age- and gender-matched mice (8-12 weeks old) were randomly allocated to different experimental groups based on their genotypes.The experimental model of acute colitis induced by DSS (4% wt/vol, 36,000-50.000 kD; MP Biomedicals, LLC, Aurora, OH) was administered in drinking water ad libitum for seven days, following four days of normal distilled water. Control mice were fed with distilled water for 11 days. Fecal samples were collected at 9 days.  DNA sequencing of the V3 and V4 region of the bacterial 16S rRNA gene was performed on a MiSeq instrument (Illumina, San Diego, CA) in paired-end, ~460 bp format at the Genomics Core of the University of North Dakota.