GSM5373927: CD8TcmRest_ctrl_TCF1_r1; Mus musculus; ChIP-Seq
WT Tcm cells were sort-purified from the spleen, crosslinked and immunoprecipitated with anti-Tcf1 antiserum anti-Tcf1 is a house-made rabbit anti-Tcf1 polyclonal antibody, which is generated by immunization of rabbits with purified full length mouse Tcf1 protein. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.
GEO Accession
GSM5373927