GSM6294025: 5/6Nx_Ctrl. [R2021-001594]; Rattus; RNA-Seq
RNA extraction was performed on cryogenically pulvarized tissue using the standard Trizol/chloroform extraction method. Automated library construction was performed by the Genomic Sciences and Precision Medicine Center (GSPMC) at the Medical College of Wisconsin using Illumina TruSeq stranded mRNA library prep kit Individual libraries were prepared for each tissue with unique dual 8 base pair indexes for samples on each sequencing run