single cell RNA seq of wild type mus musculus: lung tumor
Tumors and normal adjacent tissue of two WT and two KO mice were macrodissected and disassociated using the Miltenyi Biotec gentleMacs dissociator and the mouse tumor dissociation kit (Miltenyi Biotec  #130-096-730). Tissue pellets were strained through 100 mm then 70 mm filters (Stemcell Technologies #27217 and 27216) and RBC lysis performed per manufacturers protocol (RBC lysis buffer, Gibco #A10492-01ACK). Cells were stained with propidium iodide (Sigma #P4864). 10,000 viable cells were captured for each tissue. scRNA-seq libraries were prepared using the 10X Chromium Single Cell Platform (10X Genomics #1000006, 1000080, and 1000020) following the manufacturer's protocol. The libraries were sequenced using the NovaSeq 6000 with 150 bp paired end reads. RTA (version 2.4.11; Illumina) was used for base calling and analysis was completed using 10X Genomics Cell Ranger software v2.1.1.