3 instar-F colony-16:8-R2
For transcriptome sequencing, total RNA from third and fifth instar larvae of  F colony were collected and extracted using TRIzol reagent (Invitrogen, CA, USA). Sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), and sequencing was also performed on the Illumina NovaSeq 6000 platform.