GSM5242677: PROSER1KO1D3_WGBS_rep2; Homo sapiens; Bisulfite-Seq
Genomic DNA of cells was extracted by using the QIAamp DSP DNA Mini Kit (Qiagen, 61304). Genomic DNA was bisulfite converted using the EZ-96 DNA Methylation-Gold MagPrep (Zymo Research). Libraries were prepared from converted DNA using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences). Libraries were analyzed for insert size distribution on a 2100 BioAnalyzer High Sensitivity kit (Agilent Technologies), 4200 TapeStation D1000 ScreenTape assay, or Caliper LabChip GX DNA High Sensitivity Reagent Kit (PerkinElmer). Libraries were quantified using the Quant-iT PicoGreen ds DNA assay (Life Technologies) or low pass sequencing with a MiSeq nano kit (Illumina).
GEO Accession
GSM5242677