GSM5768304: manR mutant grown on M9+Fru; Vibrio cholerae O1 biovar El Tor str. N16961; RNA-Seq
After fixing the cells with the same volume of 100% methanol for 1 h at -20°C, total RNA was isolated using the TaKaRa MiniBEST Universal RNA Extraction Kit (#9767A, Takara Bio) according to the manufacturer's instructions. Library was constructed using NEBNext® rRNA Depletion Kit (Bacteria) (#E7850) and NEBNext Ultra II RNA library Prep kit for Illumina (#E7770) following manufacturer's instructions.