Illumina HiSeq 2000 sequencing; Whole transcriptome profiling of beta cell development in the mouse
Whole transcriptome profiling of beta cell development in the mouse
See file RNA_extraction_protocol_for_FACS-isolated_cells.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
Experimental Factor: cell type
Pdx1-expressing foregut endodermal cell
Experimental Factor: developmental stage
embryonic day 9.5