GSM4759318: MDA-MB-453 DMSO; Homo sapiens; ChIP-Seq
Cell lines were washed with PBS and crosslinked with 1% paraformaldehyde (Thermo Scientific #28906) for 10 minutes. Cross-linked material was resuspended in 0.1% SDS (50 mM Tris-HCl pH8, 10 mM EDTA) and sonicated for 5 minutes with a Covaris E220 instrument (5% duty cycle, 140 Peak Incident Power, 200 Cycles per burst, 1 mL AFA Fiber milliTUBEs) or a Covaris S2. Soluble chromatin (5 µg) was immunoprecipitated with Protein A/G Dynabeads (Thermo Fisher 10002D, 10004D) and 10 μg of H3K27ac (Diagenode C15410196), ChIP-seq libraries were constructed using Accel-NGS 2S DNA library kit from Swift Biosciences or NEBNext® UltraTM II kit from New England Biolabs.
GEO Accession
GSM4759318