16S rRNA Amplicon Sequencing of bacteria:BS-No Rpf3
PCR amplificationwas performed using general bacterial primers 341F (50-CCTACGGGNGGCWGCAG-30) and 805R (50-GACTACHVGGGTATCTAATCC-30). High-throughput sequencing analysis was conducted using an Illumina Miseq 2 _x0004_ 300 platform (Illumina, USA).