RNA-seq of LCM Medicago truncatula inner cortex at xylem poles 48 hpi
RNA was purified from laser microdissected fixed tissues using the InVitrogen RNAqueous-Micro Total RNA Isolation Kit. A total of 1 ng was used with the Takara SMART-Seq v4 Ultra Low Input RNA Kit to produce amplified cDNA. A library was created from the cDNA using the Illumina Nextera XT DNA Library Preparation Kit.