Illumina HiSeq 2500 sequencing; Whole genome bisulfite sequencing of bovine morula derived from metabolically divergent dairy cows
Whole genome bisulfite sequencing of bovine morula derived from metabolically divergent dairy cows
Morulas were collected at day 7 through uterine flushing. Morulas were washed and pooled in groups of 5. The samples were snap frozen and kept at -80 °C until use. morulas derived from in vitro produced 2-4 cell embryos and transferred into the oviduct of either metabolically profiled lactating cows or nulliparous heifers (as a metabolically unchallenged control) until day 7. Molulas were lysed and bisulfite treated using the EZ-DNA Methylation-Direct Kit (Zymo Research) following manufacturer's instructions.  Sequencing libraries were generated from the bisulfite treated DNA using the Pico Methyl-Seq Library Prep Kit (Zymo) according to manufacturer's recommendations with 20µM PrepAmp primer mix and 10-cycle PCR amplification.
Experimental Factor: growth condition
metabolically profiled lactating mother