RNA-Seq of Soybean seeds.
The messenger RNA was enriched by Oligo(dT)-attached magnetic beads, fragmented into short pieces fragments, and converted into complementary DNA (cDNA) by reverse transcription. The cDNA fragments were further amplified by PCR, and purified by Ampure XP Beads. The 150-bp pair-end (PE150) sequencing was conducted on a MGISEQ-2000 platform (BGI-Shenzhen, China).