RNAseq of North American bufonid parotoid glands
RNA quality and size distribution on an Agilent 2100 Bioanlayzer (Agilent Technologies, Santa Clara, CA) using samples that met a RIN 7.0 for subsequent analysis. We then prepared complimentary DNA (cDNA) libraries using a KAPA mRNA Hyper Prep kit (Roche, Indianapolis, IN), indexed each sample, and multiplexed twelve samples to a pool. Pools were then sent to NovoGene for sequencing on a HiSeq2500 for 150bp paired-end reads or to the North Texas Genome Center  for sequencing on a NovaSeq6000  for 100bp paired-end reads.