GSM5741719: Hypothalamus Anestrous Lib17; Ovis aries; RNA-Seq
Samples from HT and PT tissues were isolated and rapidly frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from approximately 0.5 g of tissue using RNeasy Lipid Tissue mini kit from Qiagen following the manufacturer's instructions. Only RNA samples with a RIN > 7 were used for library construction. Sequencing was carried out at the CNAG through the Illumina Hi-Seq 2000 platform.