NIBGE triple gene (NIBGE E20-01) genome
The qualified genomic DNA sample was randomly fragmented by Covaris technologyand the fragment of 350bp was obtained after fragment selection. The end repair ofDNA fragments was performed and an "A" base was added at the 3'-end of each strand.Adapters were then ligated to both ends of the end repaired/dA tailed DNA fragments,then amplification by ligation-mediated PCR (LM- PCR ), then single strand separationand cyclization. The rolling circle amplification (RCA) was performed to produce DNANanoballs (DNBs). The qualified DNBs were loaded into the patterned nanoarrays and