Illumina HiSeq 2000 sequencing; Whole transcriptome profiling of beta cell development in the mouse
Whole transcriptome profiling of beta cell development in the mouse
Total RNA was isolated using TRIzol LS (Invitrogen) and column purification (Zymo Research), according to manufacturers instructions. RNA was DNase-treated (Ambion) and integrity was assessed on the Bioanalyzer (Agilent). See file RNA_extraction_for_FACS.doc. As per manufacturer instructions. The library was constructed using the Illumina TruSeq DNA sample prep kits A&B, FC-121-1001 and FC-121-1002 with the standard Illumina methods (Bentley 2008).
Experimental Factor: cell type
neurogenin-3-expressing endocrine progenitor cell
Experimental Factor: developmental stage
embryonic day 15.5