GSM5242376: SPC+_DMDO_3; Homo sapiens; RNA-Seq
Total RNA of SPC-GFP+ cells isolated by FACS was extracted using RNeasy micro (Qiagen). 10 ng of total RNA was reverse-transcribed and amplified with 8 cycles using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech). The amplified cDNAs were used to prepare sequence libraries using Nextera XT DNA Library Preparation Kit (illumina)
GEO Accession
GSM5242376