GSM6293376: H1-TKO mESCs, RNH MapR, replicate 2; Mus musculus; OTHER
GST-MNase and GST-RH-MNase protein expression and purification were carried out as described in Yan et al. (2019). MapR experiments were performed exactly as described in Yan et al. (2019) by exposing 5 millions of cells immobilized on Concavalin A-coated beads to 1uM of purified GST-MNase or GST-RH-MNase proteins. MNase-released DNA fragments ranging between 200-1000 bp were size- purified from agarose gels using QIAquick gel extraction kit (Qiagen). Libraries were generated with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer's recommendations and sequenced by 2x50 pair-end reads on a NovaSeq platform at Fundación Parque Científico de Madrid.
GEO Accession
GSM6293376