GSM5243058: ZFP541-KO_ZFP541_rep1; Mus musculus; ChIP-Seq
The enriched pachytene and diplotene spermatocytes were cross-linked with 1% formaldehyde for 10 min and then stopped by adding 10× glycine solution. Chromatin samples were prepared with Chromatin IP Kit (CST, 9005S) according to the manufacturer's guidelines. Specific antibodies were incubated with chromatin samples overnight at 4 °C. Antibodies used are: Control rabbit IgG (from ChIP kit) and rabbit-ZFP541 (Epitope: 1-300 aa). The protein-DNA complexes were immobilized on 30μL protein A/G beads at 4℃ for 4 hrs, and then washed sequentially in low salt buffer for three times, high salt buffer for one time, and then eluted with Elution buffer at 65℃ for 30 mins. De-crosslinking was carried out in elution buffer (with proteinase K) at 65 °C overnight. DNA samples were purified with DNA Purification Buffers and Spin Columns (CST, 14209S). Immunoprecipitated and input DNAs were analyzed by deep sequencing. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit
GEO Accession
GSM5243058