GSM5060736: MM.1S_TAF3spikein_JQKD82_ChIPseq; Homo sapiens; ChIP-Seq
MM.1S cells were crosslinked, lysed and sonicated. For ChIP with reference exogenous genome, spike-in chromatin (Active Motif, 53083) was then added. The protein-bound DNA fragments were then immunoprecipitated with specific antibodies. After reverse-crosslinking, DNA was purified using QIAquick PCR Purification Kit (Qiagen). Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) or the Illumina TruSeq ChIP Library Preparation Kit according to the manufacturer's instructions.
GEO Accession
GSM5060736