16s RNA - human surface skin - 2
To profile skin microbiota, sequencing was conducted by RTLGenomics (Lubbock, TX, USA). Briefly, DNA was extracted using Qiagens MagAttract PowerSoil DNA Isolation on the Thermo Kingfisher 96-well extraction robot following manufacturers instructions. Sample amplification for sequencing was conducted using primers encompassing variable regions 1 through 3 of the 16s rRNA gene as previously described (Phillips et al. 2017) including negative control. Sequencing was conducted on the Illumina MiSeq platform (Illumina, San Diego, CA) using manufacture protocol and targeting a minimum depth of 10,000 taxonomically classified reads per sample