GSM5242391: Non_target_0H_2; Homo sapiens; RNA-Seq
For RNA-seq, total RNA was extracted using Maxwell® RSC simplyRNA Tissue Kit (Promega) and quantified using RNA 6000 Nano Kit (Agilent Technologies) in Bioanalyzer. 1µg of total RNA was subjected to oligo-dT selection (TruSeq Stranded mRNA Preparation Kit ,Illumina). PolyA-selected RNA-seq libraries were prepared using TruSeq Stranded mRNA kit (Illumina, #20020594) following manufacturer's instructions. Both TruSeq kits add Illumina-compatible adapter and indexes for sequencing.  Libraries were checked for quality (Bioanalyzer) and quantity (Qubit). Equimolar concentrations of libraries were pooled, requantified and sequenced as 50bp single reads on the Illumina HiSeq 2500 instrument.
GEO Accession
GSM5242391