GSM5243244: T12_R4; Homo sapiens; RNA-Seq
Total RNA was extracted using the PARIS kit supplied by Life Technologies (AM1921). Extracted RNA was treated with DNAse supplied by Life Technologies (AM1906), and checked for quality using RNA ScreenTapes on a 4200 TapeStation system (GS2991AA), both supplied by Agilent Technologies, before proceeding to cDNA synthesis.    cDNA was synthesised using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads, following NEB's   Protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490).    Sequencing libraries were prepared using the aforementioned kit and individual index primers for each sample (supplied by NEB).    Individual libraries were checked for quality using Qubit fluorometry (Life Sciences), as well as on the 4200 TapeStation using the High Sensitivity D1000 ScreenTape assay.    Libraries were sequenced using an Illumina NextSeq 500 sequencing machine.
GEO Accession
GSM5243244