GSM5370916: Whole Nerve 7d post-crush 1; Rattus norvegicus; RNA-Seq
A minimum of 10 sciatic nerves was used for the generation of each sample of purified cells. Schwann cells were acutely purified using immunopanning from the injured or uninjured Rat sciatic nerve according to (Lutz, 2014). For injured nerves, only the nerve segment distal to the site of crush injury was included. For uninjured nerves the anatomically equivalent nerve segment was isolated. RNA from whole nerves was purified according to the RNeasy Fibrous Tissue kit. For injured nerves, only the nerve segment distal to the site of crush injury was included. For uninjured nerves the anatomically equivalent nerve segment was isolated. Total RNA that passed QC standards proceeded to library synthesis using the Ovation® RNA-seq system V2 (Nugen 7102).
GEO Accession
GSM5370916