RIP-seq of Arabidopsis: RBP45d RIP biological replicate 1
RNA were extracted by using the TRIzol reagent from immunoprecipitated fractions of RBP45dOE (IP) or WT(mock) plants. RNA fragments ranged from 200-1000 nt in size were collected for library preparation by using TruSeq Stranded mRNA mRNA kit according to the manufacturers protocol but modified by ligating molecular identifiers (MIDs) on i5 adapter. The products were purified and enriched with 10 cycles of PCR to create the final double-stranded cDNA library.