GSM5903396: 6 month 5xFAD (replicate 2); Mus musculus; RNA-Seq
Total RNA was extracted by RNAeasy Mini kit (Qiagen). For mouse hippocampus or cortex, total RNA was extracted by RNA-easy Lipid Tissue Kit (Qiagen) as described in the manual, including with DNAse I digestion to remove contaminated DNA. RNA sequencing libraries were prepared with the TruSeq Stranded mRNA Library Prep Kit (Illumina), including poly(A) RNA purification. Libraries were then quantified using the Quant-iT PicoGreen dsDNA assay (Thermo Fisher) and MiSeq Reagent Kit Nano v2 (Illumina). One hundred cycle paired-end sequencing (100 bp) was performed on an Illumina HiSeq 2500.