RNA-seq of Furipterus horrens: adult male tongue
The tissue samples were washed from the RNAlater solution, placed in Safe-Lock tubes (Eppendorf) containing one 5mm carbide tungsten bead, and ground in the TissueLyzer II (QIAGEN). The total RNA was extracted using the RNeasy Mini kit (QIAGEN) following the manufacturer's instructions. RNA Integrity Number of all samples was above 9. Libraries were prepared using messenger RNA molecules after capturing with oligo-dT, using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA), following the manufacturer's instructions.