RNA-seq of P. patens control 24h-3-fw
8- and 24-h-after treatmen colonies were recovered, frozen using liquid nitrogen, and pulverized. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Germany). RNA quality control, library preparation and sequencing were performed by Macrogen Inc. (Seoul, Korea). Briefly, RNA integrity number (RIN > 9) was checked using an Agilent Technologies 2100 Bioanalyzer (Agilent Technologies) and cDNA libraries were prepared for paired-end sequencing using 1 g of RNA and the TruSeq Stranded Total RNA Plant LT Sample Prep. Finally, sequencing was performed on Illumina platform (Illumina, California, USA) to generate paired-end 150 bp reads, obtaining ~50 M reads per sample with Q20 > 97.5 %.