BARBEKO library Gene fitness screening in HeLa at MOI3 replicate 2 Day 0
BBK, HeLa, fitness, MOI3, Day 0; The genomic DNA of each replicate was extracted from cells using the DNeasy Blood and Tissue kit (Qiagen) and the sgRNA regions were PCR amplified by Q5 Hot Start High-Fidelity DNA Polymerase (NEB) with 26-28 cycles of reaction using several pairs of primers. The PCR products were pooled together and purified with DNA Clean & Concentranter-5 (Zymo Research Corporation), followed by NGS analysis