16S rRNA gene amplicon of bovine: rumen liquid
Rumen samples were collected 3-4h after morning feeding using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For microbial amplicon sequencing, universal primers targeting 16S rRNA gene V4 region were used (Caporaso et al., 2011). Libraries were prepared and sequenced on Illumina MiSeq platform using 2 x 300 bp chemistry.