RIP-seq of Physcomitrium patens: PTB2 RIP biological replicate 2
Immunoprecipitated RNA is subjected to library construction. Libraries are prepared by using the Illumina TruSeq stranded mRNA kit similar to the manufacturers protocol but modified by ligating molecular identifiers (MIDs) on i5 adapter. The products were purified and enriched with 10 cycles of PCR to create the final double-stranded cDNA library.