GSM5325198: SEV S2_IP; Macaca mulatta; OTHER
Total RNA was extracted using TRIzol reagent (Invitrogen, USA). Approximately 20 μg of total RNA from each sample was fragmented,and RNA fragments incubated with the m6A antibody-  Protein A/G magnetic beads. The MeRIP libraries using eluted RNA were constructed using SMARTer Stranded Total RNA-Seq Kit version 2 (Takara/Clontech) according to the manufacturer's protocol. Briefly, the eluted m6A RNA and input RNA were directly conducted first-strand cDNA synthesis without fragmentation. After that, all following steps were based on the manufacture of the SMARTer Stranded Total RNA-Seq Kit version 2. Libraries for IP RNA were PCR amplified for less than 16 cycles and input libraries were less than 12 cycles. All libraries were analyzed by an Agilent 2100 Bioanalyzer (Agilent Technologies) and quantified by real time PCR before sequencing.
GEO Accession
GSM5325198