GSM5242402: Whole fetal liver scRNA sequencing; Mus musculus; RNA-Seq
For whole fetal liver single cell RNA sequencing, fetal liver tissues were isolated from E14.5 C57BL/6 pregnant mice and digested by dissociation enzyme (Accutase) for 10 min at room temperature. Red blood cells were removed using 1×RBC lysis buffer at 4 °C. Then, cells were passed through a 40 μm strainer and re-suspended in cold DPBS with 0.04% (wt/vol) BSA. For sorted HSC single cell RNA sequencing, fetal liver tissues were isolated from E14.5 C57BL/6 pregnant mice and in cold 1×PBS dissociated into single cell suspension by pipetting up and down through 15 mL pipette. Red blood cells were removed using 1×RBC lysis buffer at room temperature for 5 min. Cells were incubated for 5 minutes at room temperature and mixed constantly by inverting the tube. After that, cells were centrifuged for 5 minutes then the supernatant was carefully removed without disturbing the pellet cells. Cells were resuspended in cold 1×PBS with 0.5% BSA, passed through a 40 μm strainer. RNA libraries were prepared with Chromium Single Cell 3′ Reagent Kits (v2) Kits according to the manufacturer's protocol.
GEO Accession
GSM5242402