GSM6293725: BYL-non-Rel-01_499213-1R; Mus musculus; RNA-Seq
Tumors of WAP-Cre/PIK3CAH1047R transgenic mice (vehicle or BYL719 treated animals) were collected, snap frozen in liquid N2, and then grinded into powder with a mortar. Total  RNA was extracted with a RNeasy Plus Mini kit (Qiagen #74136). RNA quality was analyzed on an Agilent 2100 Bioanalyzer (Agilent Technologies). The library was prepared with the Illumina TruSeq stranded mRNA-seq preparation kit (Illumina #20020595) and the TruSeq RNA CD Index Plate (Illumina #20019792) according to the manufacturer's protocol. Size and quality of the library was evaluated on the Fragment Analyzer (Advanced Analytical) with the Standard Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, #DNF-473). Then samples were pooled in equimolar ratios. Single-end libraries were sequenced with an Illumina HiSeq 2500 (50-nt read length).