GSM6257142: SW1463-GFP2P; Homo sapiens; OTHER
For RNA sequencing, total RNA was isolated using Trizol-Chloroform extraction (see section RNA isolation). RNA integrity was assessed with the Tapestation RNA ScreenTape Assay (Agilent Technologies). libraries were prepared using the KAPA mRNA Hyperprep with RiboErase (Roche) according to manufacturer's recommendation from 1 ug total RNA. Barcoded samples were multiplexed sequenced using single-end 50 bp reads on the Illumina HiSeq4000 platform.