GSM6290630: TL_MOLM14_UT_unlab; Homo sapiens; RNA-Seq
RNA was harvested with Trizol reagent, followed by chloroform extraction and isopropanol precipitation. For TimeLapse-seq (TL-seq) genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. RNA for TimeLapse-seq RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistry using mCPBA as the oxidant as described in Schofield et al. Nature Methods 2018. TL-seq libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413).