GSM5373460: cdMipPs + VPA, rep82; Homo sapiens; RNA-Seq
CdMiPs were sorted single-cell by FACS in 96-well plates in RLT buffer. mRNA was isolated using the genome and transcriptome sequencing protocol CdMiPs were sorted single-cell by FACS in 96-well plates in RLT buffer. mRNA was isolated using the genome and transcriptome sequencing protocol The libraries were prepared with the Nextera XT library prep and index kit (Illumina). 100pg of cDNA was tagmented by transposase Tn5 and amplified with dual-index primers (i7 and i5, 14 cycles). In total 279 single-cell libraries were pooled together and sequenced single 50bp on a single lane of a HiSeq4000 (Illumina). SmartSeq2 single cell RNA-Seq
GEO Accession
GSM5373460