Illumina NovaSeq 6000 paired end sequencing; Chicken transcriptome and gene expression atlas
mRNA-Seq transcriptomic experiment on an Illumina Novseq 6000
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
project
FAANG
secondary project
FAANG
assay type
RNA-seq of total RNA
sample storage
frozen, -70 freezer
sample storage processing
cryopreservation in dry ice (dead tissue)
sampling to preparation interval
24.0
experimental protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
extraction protocol
https://data.faang.org/api/fire_api/assays/ROSLIN_SOP_GENESWITCH_WP1_CHICK_EXTRACTION_DNA_RNA_20201111.pdf
library preparation location
GENEWIZ Global Headquarters, 115 Corporate Boulevard, South Plainfield, NJ 07080, USA
library preparation location longitude
-74.41963
library preparation location latitude
40.5683
library preparation date
2020-10-23
sequencing location
GENEWIZ Global Headquarters, 115 Corporate Boulevard, South Plainfield, NJ 07080, USA
sequencing location longitude
-74.41963
sequencing location latitude
40.5683
sequencing date
2020-11-04
experiment target
RNA
rna preparation 3 adapter ligation protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
rna preparation 5 adapter ligation protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
library generation pcr product isolation protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
preparation reverse transcription protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
library generation protocol
https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_mRNAseq_library_preparation_20201123.pdf
read strand
mate 2 sense
rna purity 260-280 ratio
2.13
rna purity 260-230 ratio
1.4
rna integrity number
10.0