Paired-end Illumina sequencing of C. botulinum FE9508BRB fecal isolate from botulism case
Single colony grown O/N at 35 degrees in 5mL TSB-YE and pellet resuspended in Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with Lysozyme and RNAseA treatment. NexteraXT library prep; paired-end (2x300) sequencing using MiSeq and v3 chemistry