GSM5965396: rep3_16h_PBS [DRN-21439]; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Plus Micro Kit (Qiagen, #74034) and resuspended in 14 µl nuclease-free water. RNA quantity and quality were assessed with a RNA 6000 Pico chip (Agilent, #5067-1513) on a 2100 Bioanalyzer (Agilent). Libraries were generated using the QuantSeq 3' mRNA-seq Library Prep Kit FWD for Illumina (Lexogen A01173) with the UMI second strand synthesis module in order to identify and remove PCR duplicates, following the protocol defined by the manufacturer. Library quantity and quality were assessed with Qubit 1X dsDNA HS Assay Kits (Invitrogen Q33231) and Bioanalyzer High Sense DNA chip (Agilent 5067-4626). Libraries were pooled in equimolar ratios for sequencing.