GSM5492202: M2c Macrophages, donor 1; Homo sapiens; RNA-Seq
Total RNA was then extracted with the RNeasy Plus Micro Kit (Qiagen) and stored at -80°C until use Full length cDNA were generated from 5 ng of total RNA  using Clontech SMART-Seq v4 Ultra Low Input RNA kit for Sequencing (Takara Bio Europe, Saint Germain en Laye, France) according to manufacturer's instructions with 9 cycles of PCR for cDNA amplification by Seq-Amp polymerase. Six hundreds pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, CA) followed by 12 cycles of library amplification. Following purification with Agencourt AMPure XP beads (Beckman-Coulter, Villepinte, France), the size and concentration of libraries were assessed by capillary electrophoreris.
GEO Accession
GSM5492202