GSM4759258: MCF7_2 DMSO_Fra2; Homo sapiens; ChIP-Seq
For ChIP, cells crosslinked with two agents starting with 2 mM DSG (Pierce) for 45 minutes at room temperature followed by 1 mL of 1% formaldehyde for 10 minutes. Cross-linked cell lines or tissues were quenched with 0.125 M glycine for 5 minutes at room temperature. Cross-linked material was resuspended in 0.1% SDS (50 mM Tris-HCl pH8, 10 mM EDTA) and sonicated for 20 minutes with a Covaris S2.Thirty micrograms of soluble chromatin were immuoprecipiated with Protein A Dynabeads and 1 ?g of Fra2 (CST 19967) antibody. ChIP-seq libraries were constructed using NEBNextｮ UltraTM II kit from New England Biolabs.
GEO Accession
GSM4759258