GSM5242413: N711; Homo sapiens; RNA-Seq
Single-cell suspensions were loaded in the BD Rhapsody™ Cartridge, which could effectively capture and separate single cells. Then, we loaded magnetic beads carrying a cell-specific sequence and unique molecular index (UMI) into the cartridge. In each well, a single cell was lysed, and the released mRNA was captured by a barcoded bead. All beads carrying mRNA were collected in an Eppendorf tube.Then we conducted cDNA synthesis, transcriptome amplification and library amplification via PCR with a cDNA kit. The libraries were analyzed on the Illumina HiSeq PE150 platform.
GEO Accession
GSM5242413