Feasibility and clinical utility of whole genome profiling in pediatric and young adult cancers: Sample IID_H158186_N01_01_WG01
Whole Genome Sequencing: PicoGreen quantification and quality control by Agilent BioAnalyzer, 500ng of genomic DNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog # 500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with modifications. Libraries were subjected to a 0.5X size select using aMPure XP beads (Beckman Coulter catalog # A63882) after post-ligation cleanup. PCR-free libraries were pooled equivolume for sequencing.
alignment_software
BWA MEM (v 0.7.17-L2)