RNA-Seq of mus musculus: adult male dorsal skin
Cleaned V3-V4 DNA was sent to the OUHSC Laboratory for Molecular Biology and Cytometry Research (LMBCR) for multi-plex, 2x300bp paired end sequencing on the Illumina MiSeq using standard protocols. Libraries were generated using the standard Illumnia 16s sequencing workflow and results were analyzed using the CLC Microbial Genomics Module.  Briefly, raw sequence reads were analyzed using CLC Microbial Genomics Module Amplicon based analysis workflow.  First adapter trimming and OTU clustering were performed with total reads from each sample.  Unique OTUs were compared to the GreenGenes 16s rRNA gene database and taxonomic identifications were made.