Gastrointestinal nematode metabarcoding from Australian cattle faeces: FECPAKG2 eggs stored in lysis buffer for 0 days (EF047)
PCR amplicons (25) were submitted for Illumina NGS at the Ramaciotti Centre for Genomics, University of New South Wales, Sydney, Australia. Here, Stage-2 indexing PCRs (involving the attachment of Illumina sequencing adaptors and dual index barcodes using the Nextera XT Index Kit V2), library quantification, normalisation, pooling, and denaturing were performed according to Illumina 16S Metagenomic Sequencing Library Preparation (Illumina Inc., USA). Sequencing runs were undertaken on an Illumina MiSeq platform with 2x250bp paired end reads.