Feasibility and clinical utility of whole genome profiling in pediatric and young adult cancers: Sample IID_H158916_T01_01_WT01
Whole Transcriptome Sequencing: After RiboGreen quantification and quality control by Agilent BioAnalyzer, 18ng-1µg of total RNA with an RNA integrity number varying from 1 to 9.9 underwent Ribosomal depletion and library preparation using the TruSeq Stranded Total RNA LT Kit (Illumina catalog # RS-122-1202) according to instructions provided by the manufacturer with 8 cycles of PCR.
alignment_software
STAR (v2.5.4b_ensemble_75)