GSM5370920: doxorubicin-sensitive human ovarian cancer cell line A2780_rep3; Homo sapiens; RNA-Seq
Total RNA, including miRNA, will be isolated from fresh frozen tissue or cell pellet using the Qiagen miRNeasy Mini Kit.  The cell samples are homogenized in QIAzol Lysis Reagent and after addition of chloroform, the homogenates are separated into aqueous and organic phases by centrifugation. RNA partitions to the upper, aqueous phase, while DNA partitions to the interphase and proteins to the lower, organic phase or the interphase.  The upper, aqueous phase is extracted, and ethanol is added to provide appropriate binding conditions for all RNA molecules greater than 18 nucleotides. The sample mixture is applied to an RNeasy Mini spin column, where the total RNA binds to the membrane and phenol and other contaminants are efficiently washed away.  High quality RNA (including miRNA) is eluted with nuclease-free water. The purified RNA is stored at -80°C.
GEO Accession
GSM5370920