GSM5768882: H2B5_1-MB-MD; Apis mellifera; RNA-Seq
RNA extractions of brain regions were performed with the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems) according to the manufacturer's specifications, including a DNase treatment (Qiagen) to remove genomic DNA. RNA-sequencing libraries were prepared with the KAPA stranded mRNA kit (Roche) following the manufacturer's protocol, except that we appended TruSeq unique dual indexes (UDIs, Illumina) instead of the adapters provided by the kit to better control for index hopping during sequencing.