DNA-seq of Homo sapiens: Molm-14 cells
Dual indexed illumina Libraries were generated using two-step PCR procedure. 1st PCR was applied to target the regions of interest. Following dilution of the 1st PCR products with nuclease free water (1:1000), a 2nd PCR was performed using primers composed of Illumina sequencing primers, indexes and adapters. Barcoded 2nd PCR products were pooled together at equal volume. Pooled library sizes were selected (2% gel, BluePippin, Sage Science) and sent for 2  150-bp deep sequencing.