Poxvirus in house finches (Haemorhous mexicanus): genome sequence analysis and patterns of infection in wild birds
A pox lesion of ~5mm x 5mm was homogenized in 300ul of SM buffer and this homogenate was used to extract viral DNA using the High Pure Viral nucleic acid kit (Roche Diagnostics, USA). This resulting DNA extract was used to construct a 2x150 nt library that was sequenced on an Illumina NovaSeq 6000