16S amplicon sequncing of reactive barriers developed for managed aquifer recharge: 50%comp barrier, location C, day 78, replicate 1
Reactive barriers were created by packing Plexiglas columns with either pure sand, or a mixture of sand with 10% or 50% compost. Samples were taken from drained columns and DNA was extracted using the DNeasy PowerSoil kit. Amplicons of the 16S rRNA gene were amplified with the primers pro341F/pro805R and sequenced on the Illumina MiSeq platform (2___250 bp).