RNA-Seq of Mammalian orthoreovirus 5
RNA was extracted using a QIAamp Viral RNA Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturers protocol. A cDNA library was generated using a NEBNext Ultra RNA Library Prep Kit (Illumina, San Diego, CA, USA) and sequenced on an Illumina MiSeq sequencer. De novo assembly was performed using SPAdes 3.5.0.