GSM6256946: ChIP DE H3K27me3, replicate B; Homo sapiens; ChIP-Seq
ChIP experiments were performed on chromatin extracts according to the manufacturer's protocol (MAGnify ChIP, Life Technologies Cat. n. 492024). For each immunoprecipitation reaction, 10ug of sheared chromatin from DE and PE  differentiated cells was used (two biological replicates per ChIP experiment). Sheared chromatin was incubated O.N. with 5μg of anti- H3K27me3 (Abcam Cat. n. ab6002), H3K4me3 (Active Motif Cat. n. 39159), H3K27ac (Active Motif Cat. n. 39133), or H3K4me1 (Abcam ab8895) antibodies. Immunoprecipitated (IP) and input DNA samples were quantified by Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and the DNA integrity was checked with 4200 TapeStation (Agilent Technologies, Palo Alto, CA, USA). NEBNext Ultra DNA Library Preparation kit was used following the manufacturer's recommendations (Illumina, San Diego, CA, USA). Briefly, the ChIP DNA was end-repaired and adapters were ligated after adenylation of the 3' ends. Adapter-ligated DNA was size selected, followed by clean up, and limited cycle PCR enrichment. The ChIP library was validated using Agilent TapeStation and quantified using Qubit 2.0 Fluorometer as well as real time PCR (Applied Biosystems, Carlsbad, CA, USA).