RNA-seq of Pinus radiata: needle heat stress
RNA of the samples was extracted as described by Valledor et al. (2014) and then quantified in a Nabi UV/Vis Nano Spectrophotomer. RNA integrity was checked by agarose gel electrophoresis, and potential DNA contamination by PCR. cDNA was obtained from 500 ng of RNA using the RevertAid kit (ThermoFisher Scientific) and random hexamers as primers following the manufacturers instructions.