Illumina MiSeq paired end sequencing
16S metagenomic sequence, V1-V2 amplicon
V1-V2 region of 16S ribosomal DNA was amplified using primer paires of 27Fmod and 338R. Amplicon was was purified and further amplified with Nextera XT Index  primers. After purification, 4 pmol of amplicons were pooled.