Nanopore ATCC10970 plasmid
The total amount of PFGE-isolated plasmid DNA was used for library construction following the procedure of Ligation Sequencing Kit (SQK-LSK109, Nanopore Technologies) with washing step using Long Fragment Buffer (LFB) to enrich longer DNA fragments. The constructed library was loaded either on the pre-used (24 hours), washed (Flow Cell Wash Kit, EXP -WSH003) MinIon Flow cell R9 (samples M4018 and R6-500) or Flongle Flow cell (sample ATCC 10970). The cells were run for 20 hours with electrical voltage parameters set to -210 mV (pre-used MinIon flow cells) or with default parameter (Flongle Flow Cell) in MinKnow software.