The complete chloroplast genome of Miliusa glochidioides
After passing the sample genome DNA test, the DNA was interrupted by physical method (ultrasonic), and then the broken DNA was purified to construct the sequencing library. The steps are as follows: DNA terminal repair, add A to the 3 'end, connect the sequencing connector, recover the target fragment by agarose gel electrophoresis, and perform PCR amplification on the target fragment, and finally build the sequencing library. Quality inspection was carried out before sequencing, and sequencing was performed on Illumina NovaSeq6000 platform after passing the quality inspection