GSM6293402: Medium 24 h, LPS 1 h, biol rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted using a kit from Jena Bioscience (Jena, Germany, cat# PP-210) and treated with DNase (NEB, Ipswich, MA, cat# M0303). mRNA was purified using oligo-dT-coated superparamagnetic beads (NEB, cat# E7490). Barcoded cDNA libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (NEB, cat# E7765) and NEBNext® Multiplex Oligos for Illumina® (NEB, cat# E7335 and E7500) according to manufacturer's instructions.