FACS sorted oxidized (EMSH-oxidized) mutant pool replicate 2
The redox- altered Tn-mutants (oxidative-EMSH) were detected by quantifying the Mrx1-roGFP2 fluorescence ratio (excitation: 405 nm /488 nm; emission: 510 nm) of the library and sorted by using fluorescence-activated cell sorter to obtain EMSH OXD Tn.