GSM5242793: Antibody-derived tags from malignant peritoneal mesothelioma patient; Homo sapiens; OTHER
Pleural and peritoneal mesothelioma tumor tissues were treated with antibiotic rich media followed by mechanical digestion with blades followed by 5 minute incubation with 100uL of liberase at 80ug/uL at 37 degree celsius. Tissue is the passed over a 70 micron filter into RPMI media. Cells can be sorted by flow cytometry for CD45+ and CD45-EPCAM+ cells. Treatment of cells with antibody tagged transcripts from biolegend's CITE-seq panel for 30 minutes. Chromium 5 prime single-cell RNA seq kit by 10x genomics is used for creation of samples and libraries. CITE-seq with scRNAseq
GEO Accession
GSM5242793