Illumina MiSeq paired end sequencing
V5-V7 16S rRNA using primers 799F and 1193R to exclude chloroplast reads
DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V5-V7 hypervariable regions of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences.