16S amplicon : water samples from Qinghai Lake
Water microbes was filted by 0.22 um microporous membrance (millpore, USA) , and the membrane was extracted  using the FastDNA Spin Kit for Soil (MP BIO, Solon, OH, USA) according to the manufacturers protocol. The DNA concentration was measured using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific, Schwerte, Germany). The barcoded polymerase chain reaction (PCR) primers 515F/806R targeting the V4 region was used for the 22S rRNA amplification