amplicons of 16S fragments
Amplifications were performed and prepared for sequencing at RTL Genomics (Lubbock, TX). Amplification products were pooled equimolar, and each pool was size selected in two rounds using SPRIselect (BeckmanCoulter, Indianapolis, Indiana) in a 0.7 ratio for both rounds. Size selected pools were then quantified using the Qubit 2.0 fluorometer (Life Technologies) and loaded into an Illumina MiSeq (Illumina, Inc. San Diego, California) 2300 flow cell at 10pM and sequenced at RTLGenomics.