RNA-Seq of mus musculus: adult male ACC
mRNA was enriched and treated into short fragments using a fragmentation buffer, and reverse transcripted into cDNA with random primers. The cDNA fragments were purified, end repaired, added a poly(A) tail, and ligated Illumina sequencing adapters. The ligation products were then size-selected by agarose gel electrophoresis, amplified by PCR, and sequenced using Illumina NovaSeq 6000