RNA seq of  hypothalamus tissue :6 weeks old malehypertensive mouse induced by Ang II
The mice were randomized into the following groups: control, Ang , Ang  + QDG. Saline was infused in the control group, and Ang  was infused in the others using a mini-osmotic pump (Alzet 2002D, Durect Corporation, CA, USA) for 4 weeks at a rate of 500 ng/kg/min, implanted in a skin pocket between the scapulae based upon provided directions. Mice in the Ang  + QDG groups received intragastric QDG (1.145 g/kg/day) while equivalent amounts of saline were given to animals in control and Ang  groups. Treatment proceeded for four weeks. Animals were killed and the brain tissues were separated carefully and quickly split into -91 liquid nitrogen used for further research.