GSM5242690: WT1_Input; Homo sapiens; RNA-Seq
RNA was extracted according to TRIzol protocol. Small RNAs (<200nt) were first purified using the mirVana miRNA Isolation Kit (Thermo Fisher Scientific).  HNSCC samples were harvested from the tongue of mice and single cell suspensions were prepared for 10× genomics processing. Libraries were prepared according to Illumina's instructions accompanying the TrueSeq Stranded mRNA library preparation kit, TruSeq Ribo Profile (Mammalian) Library Prep Kit and the NEBNext® Small RNA Library Prep Set. scRNA-seq libraries were prepared using Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (10X Genomics) according to manufacturer's protocol.
GEO Accession
GSM5242690