GSM4759762: BAPRNAi_GAFChIP_Rep1; Drosophila melanogaster; ChIP-Seq
Cells were washed with ice cold PBS, then fixed in 1% Formaldehyde in PBS at 25 °C for 10 min. Formaldehyde was quenched with 0.125 M glycine. Anti-GAF (affinity purified, lab made) was coupled to Sheep-Anti-Rabbit M280 dynabeads (1:200 concentration). IPs were performed overnight at 4 °C, and after washing beads, crosslinks were reversed using SDS and Proteinase K overnight at 65 °C. Libraries were constructed using the NEB Next Ultra II DNA library prep kit.
GEO Accession
GSM4759762