gRNA amplicon sequencing of K562 cells infected with the Brunello gRNA library
K562 cells were transduced with the lenti-guide-puro vector. The gRNA site in lenti-guide-puro was amplified using titanium taq polymerase with primers containing P5 and P7 illumina adapters as well as a sample barcode. Products were purified using spri beads (beckman coulter) and sequenced on an illumina Next Seq