Anammox-CSTR enrich
The bacterial 16S rRNA gene of the extracted DNA was amplified using the following primers: 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) in the V3-V4 region and was further sequenced through the Illumina Hiseq2500 platform by BGI Co., Ltd. (Shenzhen, China).