Gwen Pacbio CLR Data
We sampled young leaf materials from the original Gwen tree and isolated high molecular weight genomic DNA (gDNA) using the method of (Chin et al. 2016). To avoid the co-precipitation of polysaccharides and phenolics with DNA, they were reduced in a pre-washing step with sorbitol. The purity, quantity and integrity of DNA were assessed with a Nanodrop 2000 spectrophotometer (Thermo Scientific, IL, USA), a Qubit 2.0 Fluorometer together with the DNA High Sensitivity kit (Life Technologies, CA, USA), and by pulsed-field gel electrophoresis. SMRTbell libraries were prepared as described in (Massonnet et al. 2020) and sequenced on a PacBio RS II (Pacific Biosciences, CA, USA) using P6-C4 chemistry (DNA Technology Core Facility, University of California, Davis).