GSM5242713: BM-WT-TIRAP-1; Mus musculus; RNA-Seq
Bone marrow was harvested by flushing the bones from mice that were injected with 5-FU 4 days prior to select for HSPC. The HSPC were transduced with TIRAP or control vectors. RNA was harvested from these transduced cells using Qiagen Rneasy kit. mRNA was prepared from 1ug of total RNA using the MultiMACS mRNA isolation kit and cDNA synthetised using the H Minus First Strand cDNA Synthesis Kit. The paired-end RNA sequencing library was prepared following the BCCA Genome Sciences Center paired-end library preparation plate- based library construction protocol on a Biomek FX robot
GEO Accession
GSM5242713