HAE-B2-20 MOI0.2-1
RNA with RIN 8.0 were fragmented in a fragmentation buffer for thermal fragmentation to 130-160nt. First-strand and secod-strand cDNA was generated. The cDNA product was purified by magnetic beads and end repaired by addition of adaptors, followed by several rounds of PCR amplification to enrich the cDNA fragments. Then the PCR products are purified and subjected to library quality control on the Agilent Technologies 2100 bioanalyzer. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library.