GSM5768737: RKO cells, control-1; Homo sapiens; ATAC-seq
100,000 cell nuclei were extracted using NPB buffer (10 mM Tris, pH 7.5, 10 mM NaCl, 3 mM MgCl2, and 0.1% Triton X-100) at 4 °C for 10 minutes. Tagmentation was performed in a reaction buffer (Vazyme) containing Tn5 transposase (#TD501, Vazyme, Nanjing, China) at 37 °C for 30 minutes. DNA was then extracted using a PCR purification kit (#28106, Qiagen, Hilden, Germany) DNA libraries were generated by PCR for sequencing using standard Illumina protocols.