GSM5903185: H1_Glu_0hr_rep3 [s7]; Homo sapiens; RNA-Seq
DIV35 neurons were washed once with D-PBS, lifted using Accutase and RNA was extracted with TRIzol following the provider's instructions. Following isolation, the RNA was treated with TURBO DNA-free kit. Total RNA (1μg) was used per sample for library preparation and sequencing. Standard RNA-seq with Poly(A) mRNA selection was performed by Genewiz using Illumina NovaSeq platform and yielding 20-30 million paired-end 150 bp reads per sample.