GSM6214540: 4T1_thy1.1_dox_rep1; Mus musculus; RNA-Seq
Cultures were lysed in culture well using Qiagen Buffer RLT with β-mercaptoethanol added followed by total RNA isolation using RNeasy mini spin columns (Qiagen). Library preparation was performed using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen; Cat# 015.96) according to manufacturer's protocols.