GSM5965215: puchi_12h_R1_11_03_2020; Arabidopsis thaliana; RNA-Seq
For each replicates, root bends of more than 400 seedlings were microdissected under a binocular microscope and frozen in liquid nitrogen immediately on harvesting. For reference, approximately 400 mature root segments located between the bend and the shoot were harvested in 4d-old seedlings at 9 h after an inductive gravitropic stimulation to be used as a reference of non-gravitropic- stimulated root tissues devoid of developing LRP (termed “time point 0” in the data set).Total RNA was extracted using Qiagen RNeasy plant mini kit with an on-column DNAse treatment following the manufacturer's recommendation (RNAse-free DNAse Set, Qiagen, Crawley, UK). RNA samples were quantified using a Nanodrop ND100 spectrophotometer (Nanodrop, Wilimington, USA) and RNA purity and integrity were evaluated using High-Resolution Automated Electrophoresis 2100 Bioanalyzer system from Agilent Technologies (https://www.agilent.com/en/product/automated-electrophoresis). cDNA Libraries were constructed using Stranded mRNA Prep Ligation kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions and sequenced on a complete S1 flow cell (2 lanes) of an Illumina NovaSeq 6000 in paired-end 100 nucleotides mode.