GSM5903089: AV_3; Mus musculus; RNA-Seq
Amygdalae were punched and manual and enzymatic dissociations were performed using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany). Pyramidal neurons were then sorted by using the MoFlo Astrios EQ (Beckman Coulter, California, USA) into ice-cold lysis buffer (Reliaprep RNA Cell Miniprep System, Promega, Wisconsin, USA) and then stored at -80°C until RNA extraction. RNA libraries were prepared for sequencing using standard Illumina PE protocols.