GSM5866830: Fbl+/- Raw264.7-0h-input; Mus musculus; OTHER
Total RNA was harvested using Trizol reagent. Then total RNA was used for removing the rRNAs with NEBNext rRNA Depletion Kit (NEB) following the manufacturer's instructions. RNA libraries were constructed by using a protocol from Dai Q et al., Nat Methods, 2017. with slight modification. Nm-Seq