RNA-seq of streptococcus mutans
Total RNA of three replicates from each group were extracted and purified by TRIzol Reagent (Invitrogen). RNA-seq transcriptome libraries were constructed from enriched mRNA samples using the TruSeq RNA sample prep kit (Illumina, USA) using 2g of total RNA. After quantified by TBS380, paired-end RNA-seq sequencing library was sequenced with the Illumina HiSeqTEN (2  150bp read length). The processing of original images to sequences, base-calling, and quality value calculations were performed using the Illumina GA Pipeline (version 1.6), in which 150bp paired-end reads were obtained