GSM6293762: TgTum_591633.4L; Mus musculus; ATAC-seq
gDNA of 94 untreated tumors and 51 resistant tumors (from a total of 53 mice) was isolated with a DNeasy Blood and Tissue Kit (Qiagen #69504) and fragmented by sonication (Covaris sonicator) to a size of 250 bp. DNA fragments were end repaired, A-tailed, purified and then ligated to the splikerette adaptor (annealed 5'-gttcccatggtactactcatataatacgactcactataggtgacagcgagcgct-3' with 5'-gcgctcgctgtcacctatagtgagtcgtattataatttttttttcaaaaaaa-3'). Transposon containing DNA fragments were enriched by PCR using a primer specific for the 5'-transposon arm (5'