RNA-Seq of mus musculus: PD-fed female colon fibroblasts 2
1ug of total RNA was used to construct cDNA libraries with the TruSeq RNA library kit. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 101nt paired-end sequencing by Illumina NovaSeq 6000. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2100 Bioanalyzer.