pahE-seq of bacteria: benzopyrene amended mangrove sediment at day 0
pahE genes of PAHs-degrading bacteria were amplified by four pahE primers sets from the metagenomic DNA of samples, which were taken from each PAH amended or un-amended mangrove sediment microcosms at different cultivation time. The barcodes were ligated to the reverse end of the amplicons.  PCRs were run in quadruplicate independently and purified PCR products were then mixed together for each sample. Four pahE primers sets: pahE1F&pahE1R: pahE1F 5-TGCGGCGGGTGTNAAYGGNAT-3, pahE1R 5-CCTGAGGAATCTCGGACATYTSTGCCCARAA-3; pahE2F&pahE2R: pahE2F 5- AGCATGGGAACKYTKGGNGA-3, pahE2R 5- TTTGGCGGTVACVACYTG-3; pahE3F&pahE3R: pahE3F 5- GACGGCGTSGACGGVATCAT-3, pahE3R 5- TCAGGGTTGTCRTARAKSA-3; pahE4F&pahE4R: pahE4F 5- TGGTGCGYGAYGGBGYCGA-3, pahE4R 5- GGCGTGCGGGTTSTSRTARAYCA-18;