GSM5602502: S3: NoGC2; Mus musculus; RNA-Seq
Total RNA was extracted from each sorted T cells using a RNeasy micro kit (Qiagen), and each sample was evaluated for the RNA integrity by TapeStation system (Agilent) cDNA synthesis and enrichment were performed using the SMART v4 protocol (Takara/Clontech), and library preparation was conducted using an Illumina Nextera XT library preparation kit