5 instar-F colony-13:11-R3
For transcriptome sequencing, total RNA from third and fifth instar F colony reared in short daylenth (L:D=13:11) were collected and extracted using TRIzol reagent (Invitrogen, CA, USA). Sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), and sequencing was also performed on the Illumina NovaSeq 6000 platform.