RNA-Seq of Gynandra leaves
The total RNA sample of G. gynandra leaves was used to construct a cDNA library by the High Throughput Genomics Core, Academia Sinica, Taiwan. Full-length cDNAs were synthesized and amplified using the Clontech SMARTer PCR cDNA Synthesis Kit with PrimeSTAR GXL DNA Polymerase (Takara Bio, USA). The PCR products were purified with AMPure PB magnetic beads (PacBio), end-repaired, and constructed into an Iso-Seq library by ligating to the blunt-end adaptor of the SMRTBell Template Prep Kit 1.0 (Pacific Biosciences, USA).