GSM5870335: HS27a from MCF7+HS27a Co-Culture Sample 3; Homo sapiens; RNA-Seq
To separate cancer cells from stromal cells in co-culture conditions, we used human CD326 (EpCAM) Microbeads (#130-061-101, Miltenyi, Auburn, California, USA) and LS columns (#130-042-401, Miltenyi) and followed the manufacturer's recommendations for separation. We extracted RNA using the RNeasy Mini Kit following manufacturer's instructions (#74104, Qiagen, Hilden, Germany) We prepared RNA libraries for sequencing using standard Illumina protocols