Testes of adult mice infected with ZIKV
The 10xgenomics platform first used microfluidics to separate individual cells, then encapsulated gel beads with barcode and primers and single cells in oil droplets. In the oil droplets, the gel beads dissolve and release the reverse transcription oligo, and the cell cleaved and released mRNA. The cDNA with barcode was sequenced by SMART. After the destruction of the liquid reservoir, the cDNA was constructed and detected by Illumina sequencing platform.