RNA-Seq quadriceps
Quadriceps muscles were isolated from overnight fasted or freely fed mice. Total RNA was prepared from frozen tissues. The RNA quality of all samples was analyzed on the Agilent 2100 bioanalyzer using the RNA 6000 Nano Chip. cDNA libraries were generated using New England Biolabs Ultra II kit following the manufacturers instructions. Indexed libraries of good quality (RIN > 8) were pooled and used for sequencing on a HiSeq 2500 Illumina Sequencing System. Single end 50 bp reads were mapped to mouse genome mm10.