RNA-Seq of E. coli in C. elegans
C. elegans were grown and fed only E. coli OP50. They were harvested at three different timepoints (Day 5, 7.5, and 10). RNA was extracted from the harvested C. elegans using Zymo's Quick RNA Miniprep Plus kit, and libraries were created with NuGEN's Trio RNA-Seq kit. The resulting libraries were then sequenced on an Illumina NextSeq 550.