RNA seq of Nicotiana benthamiana: grafted roots
Total RNA quantity and integrity was measured with Qubit 2.0 and 2100 Bioanalyzer (Agilent Technologies) respectively. Small RNA library was prepared using Next Ultra small RNA Sample Library Prep Kit for Illumina (NEB). The sequencing procedure was performed with illumina HiSeq Xten system at BioMarker Technologies, Beijing, China. The total sRNA reads including miRNA and unannotated RNAs were then obtained by removing sequences from other non-coding RNAs, such as rRNA, tRNA, snRNA, snoRNA, repetitive sequence using Bowtie tool to align against the Silva, GtRNAdb, Rfam and Repbase databases.