Illumina NovaSeq 6000 paired end sequencing; A multi omics analysis of a human stem cell based in vitro model of cardiac hypertrophy
A multi omics analysis of a human stem cell based in vitro model of cardiac hypertrophy
The study was conducted with cardiomyocytes obtained from Takara Bio. The cardiomyocytes are derived from Human induced pluripotent stem cell line ChiPS 22, however, the differentiation protocol is proprietary information owned by Takara Bio. Cardiomyocytes derived from induced pluripotent stem cells were exposed to endothelin-1, 10nM, for up to 72 hours. Total RNA was extracted using MagMAXâ„¢ Total RNA Isolation Kit (AM1830, Invitrogen, www.thermofisher.com) Library construction was performed using Illumina Truseq stranded total RNA with Illumina Ribozero method