RNA-Seq of Odocoileus virginianus: adult brain
Total RNA was isolated from powdered brain tissue using a commercial kit, following the manufacturers instructions (RNeasy Mini, Qiagen). RNA was precipitated with ethanol to remove salts. RNA quality was assessed via spectrophotometry (NanoDrop Technologies, Inc.) and gel electrophoresis (Bioanalyzer 2100 , Agilent Technologies). Library construction and sequencing were performed by the Centre d'Expertise et de Services of Gnome Qubec. Libraries were generated from 250 ng of total RNA. Messenger RNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were completed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Libraries then underwent 2x100bp sequencing using an Illumina HiSeq2500 platform.