GSM5139857: RB03_mRNA_rep2; Homo sapiens; RNA-Seq
Single cells of RB tumor tissues were isolated by using Neuronal Isolation Enzyme (with papain)(Thermo Fisher,#88285) The libraries were constructed using the Chromium platform and Chromium Single Cell 3′ v2 chemistry. Briefly, cellular suspensions were loaded on a Chromium Single Cell Instrument (10x Genomics, Pleasanton, CA) for GEMs Generation and Barcoding. Single cell RNA-Seq libraries were prepared using the Chromium Single Cell  Library Kit (10x Genomics, Pleasanton, CA).