GSM5284679: mHB1.fastq.gz; Homo sapiens; RNA-Seq
Total RNAs were isolated from HBs (Day 10) and HEPs (Day 17) using TRIzol™ Reagent (Thermo Fisher Scientific) and cleaned up with RNA Clean & Concentrator-5 kit (Zymo Research). Isolated RNAs were submitted to Genewiz sequencing facility for RNA-sequencing. All submitted samples had at least 9.5 of RNA integrity number (RIN). Libraries were prepared by Poly A selection from total RNA and sequenced using HiSeq 2 x 150 bp sequencing (Illumina).