Aludens_larvae in  guava
All samples were deep frozen in liquid nitrogen and pulverized for five min with a plastic pistil.  gDNA extraction was performed with the QIAamp DNA Mini Kit from QIAGEN (Hilden, Germany).  The gDNA obtained was used for the amplification of the 16S rRNA gene.  PCR amplification was performed using an enrichment strategy.  Briefly, the V3-V4 region of the 16S rRNA gene was amplified using previously described primers (Klindworth et al. 2013).  The enrichment consisted of an initial amplification of 15 cycles, starting from 300 ng of gDNA.  Then, two L of the previous amplification were taken as a template to carry out a second amplification of 25 cycles.  Reactions were performed as suggested by the Ilumina 16S Metagenomic Sequencing Library Preparation manual, but reactions were performed in a total reaction volume of 50 L.  Amplicons were purified with the Promega Wizard SV Gel and PCR Clean-Up System kit and their concentration was determined by a Qubit 2.0 Fluorometer (Thermo, USA).  Purified PCR products were indexed with Ilumina sequencing adapters using the Nextera XT Index Kit of Illumina and were purified and quantified as previously described (Klindworth et al. 2013).  The quality of the library was determined using the Agilent Bioanalyzer 2100 system.  The indexed amplicons were sequenced in a paired-end format (2 x 300 bp) using a MiSeq Reagent Kit V3 (600 cycles), on a MiSeq platform from Illumina.  Sequencing was carried out by the Sequencing Unit of the Biomimic Scientific and Technological Cluster at the Instituto de Ecologia, A.C. - INECOL.