Amplicon of bacteria: Rhizophere soil of Cynodon dactylon
The CTAB (Cetyltrimethylammonium Bromide) method was used extracting total genome DNA from bulk and rhizosphere soil samples. The distinct region 16S V4 of 16S rRNA gene was amplified using specific primer pairs 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3') with the barcode. Illumina NovaSeq sequencing was conducted in Novogene company (Beijing, China) and generated 250 bp paired-end reads.