GSM5284297: K27me3 100µM IOX1 THP1; Homo sapiens; ChIP-Seq
Trypan blue cell counts were carried out immediately prior to treatment of cells with IOX1. RNA extraction was carried out using the QIAshredder spin columns and RNeasy Plus Micro Kit or Mini kit according to the manufacturer's instructions. RNA quantities were assessed by Nanodrop and quality assessed using the Agilent RNA integrity 6000 nano kit. For ChIP-seq, DNA was purified using Diagenode's iPure kit v2. RNA-seq libraries were produced using the TruSeq stranded mRNA kit (Illumina). For ChIP-seq, libraries were made using the TruSeq ChIP Library Preparation Kit according to the manufacturer's instructions.