GSM6215815: Δnac cytidine 1 (RNA-seq); Escherichia coli K-12; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a Qubit 4.0 (Thermo Scientific Inc., Wilmington, DE, USA). RNA libraries were prepared for sequencing using standard Illumina protocols