bacterial 16S rRNA gene V3V4 amplification of sinus swab samples: adult sinus mucosa bacterial composition
total DNA was extracted from sinus swab samples, bacterial 16S rRNA gene V3-V4 region amplified, and purified using ampure beads. Purified amplicons were submitted to Auckland Genomics for library preparation using a dual-indexing approach with Nextera technology and 2x300 bp paired-end sequencing using Illumina MiSeq