GSM4975801: CD14+ monos/macs from blood of ICH patient number 2046 on day 3 of study - replicate 1; Homo sapiens; RNA-Seq
CD14 monos/macs and neutrophils were sorted from purified leukocytes preparations directly into RA1 cell lysis buffer and RNA was isolated using NucleoSpin RNA XS kit (Macherey-Nagel, #740902). Smart-Seq2 cDNA synthesis was performed with the following modifications: 1) input RNA was normalized prior to cDNA generation by diluting to ~1,000 cells per reaction, 2) reverse transcription was performed with Superscript III (ThermoFisher, #18080-085) in place of Superscript II according to the manufacturer instructions. Paired-end sequencing libraries were prepared using the Nextera XT DNA sample Prep Kit (Illumina, #FC-131) according to the manufacturer's instructions.