PacBio HiFi sequencing data of Laterallus jamaicensis: blood sample from an after-hatch-year female
The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-938-900) according to the manufacturer's instructions.  HMW gDNA was sheared to a target DNA size distribution between 15 kb  20 kb.  The sheared gDNA was concentrated using 0.45X of AMPure PB beads (PacBio, Cat. #100-265-900) for the removal of single-strand overhangs at 37C for 15 minutes, followed by further enzymatic steps of DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 10 minutes and 65C for 30 minutes, ligation of overhang adapter v3 at 20C for 60 minutes and 65C for 10 minutes to inactivate the ligase, then nuclease treated at 37C for 1 hour.  The SMRTbell library was purified and concentrated with 0.45X Ampure PB beads (PacBio, Cat. #100-265-900) for size selection using the BluePippin system  (Sage Science, Beverly, MA; Cat #BLF7510) to collect fragments greater than 9 kb.  The 15  20 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using two 8M SMRT cells, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.