Metabarcoding of environmental samples: beer
DNA was extracted from beer samples using the Qiagen Powerfoods kit, and the ITS region of fungal rRNA amplified using BITS and B583S fusion primers incorporating Illumina Nextera v2 indices and P5/P7 adapters. DNA concentrations normalized using Sequelprep plates. Pooled libraries sequenced on Miseq with 2x300 bp chemistry.