RADseq of Somniosus Somniosus
Extracted DNA of sufficient quality was digested with SbfI. Barcoded adapters were ligated to fragments before pooling individuals into libraries, which were sonicated (12-14 cycles of 30s each). A streptavidin bead wash was used to remove nontarget fragments. Illumina indices were ligated to the remaining fragments using the NEBNext Ultra DNA Library Prep Kit for Illumina End Prep. Fragments in the indexed libraries were size selected (250bp insert) prior to enrichment with PCR (12 cycles).