WGS of paddy soil metagenome
DNA library was constructed by TruSeq Nano DNA LT Library Preparation Kit (FC-121-4001) .DNA was fragmented by dsDNA Fragmentase(NEB, M0348S) by incubate at 37C for 30min.Library construction begins with fragmented cDNA. Blunt-end DNA fragments are generatedusing a combination of fill-in reactions and exonuclease activity, and size selection is performedwith provided sample purification beads. An A-base is then added to the blunt ends of each strand,preparing them for ligation to the indexed adapters.Each adapter contains a T-base overhang forligating the adapter to the A-tailed fragmented DNA. These adapters contain the full complementof sequencing primer hybridization sites for single, paired-end, and indexed reads. Single- or dualindexadapters are ligated to the fragments and the ligated products are amplified with PCR by thefollowing conditions: initial denaturation at 95oCfor 3 min; 8cycles of denaturation at 98oCfor 15sec, annealing at 60oCfor 15 sec, and extension at 72oC for 30 sec; and then final extension at72oCfor 5 min