RNA-seq of Caenorhabditis elegans: adult hermaphrodite
Total RNA was extracted from whole animals of ~100,000 synchronized young adults. PolyA-tai RNAs were enriched using Promega PolyATtract mRNA Isolation Kit (Promega). mRNA libraries were prepared according to the manufacturers protocol of the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs). Libraries were sequenced using an Illumina HiSeq4000 at the University of Chicago Genomic Facility.