Metagenome of contaminated soil
The genomic DNA was amplified at the V3V4 region of the 16S rRNA using the primers 341F and 805R for bacteria (ChunLab Inc., Seoul, South Korea). The purified amplicons of the PCR were tagged with Illumina indices and adapters from a Nextera XT Index Kit (Illumina, San Diego, CA, USA). Libraries were constructed at ChunLab Inc. using the Illumina MiSeq platform, quality of the constructed libraries were checked with Agilent 2100 Bioanalyzer System (Agilent Technologies, Palo Alto, CA, USA) using a DNA 7500 chip at ChunLab Inc. (Seoul, South Korea) and thereafter quantified using Quanti-iT PicoGreen dsDNA Assay kit (Invitrogen) according to the manufacturers instructions. Short DNA fragments were removed using Clean PCR (Clean NA, Netherlands).