GSM5966787: Mouse hypothalamus Glp1r-EYFP, sample 3 [S12_L001]; Mus musculus; RNA-Seq
Fluorescence-activated cell sorting was performed using a BD Influx Cell Sorter to isolate Glp1rEYFP+ cells. Cells were gated according to cell size (FSC), cell granularity (SSC), FSC pulse-width for singlets, fluorescence at 488 nm/532 nm for EYFP and 647/670 nm for nuclear stain with DraQ5. Single cell cDNA libraries from purified Glp1rEYFP+ cells were generated using the 10× Genomics Chromium Instrument and single-cell 3' Reagent kit (V3); R1 = 28 bp, R2 = 91 bp