GSM6297348: Growth Factor-induced hiPSC-derived HLCs Day 4 [GC117477]; Homo sapiens; RNA-Seq
RNA was extracted and quality control was performed as mentioned above.We performed RTqPCR, using progeny from 3 wells of 96 well plates for RNA extraction using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer's instructions with addition of DNAseI from the On-Column DNase I Digestion kit (Sigma-Aldrich). Library preparation was performed with the LexoGen Quant Seq. Kit. Libraries were sequenced on an Illumina HiSeq4000 sequencing system.