complete cp genome of A. moluccana
1) Building a library with an initial amount of 1g DNA;2) Covaris M220 ultrasound interrupts DNA to 300~500bp;3) Fill in, add A on the 3 end, connect the index adapter (TruSeq Nano DNA Sample PrepKit);4)Library enrichment, PCR amplification 8 cycles;5)2% agarose gel recovery of the target band (Certified Low Range UltraAgarose);6) TBS380 (Picogreen) quantify, mix according to the data ratio and put it on the machine;7) Bridge PCR amplification on the cBot solid-phase carrier to generate clusters; 8) Illunima novaseq 6000 sequencing platform for 2*150bp sequencing.