GSM5966770: nlp2-3_nlp7-1_Root_rep_1; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from root and rosette using the RNeasy plant minikit (QIAGEN) including a DNase treatment according to manufacturer's instructions. Preparation of RNA library and transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions.