GSM5141326: Hepa.ChIPseq_GR.H11.Dex24h.2; Homo sapiens; ChIP-Seq
Total RNA was extracted by using Qiagen RNeasey Mini kit. DNA was isolated using phenol/chloroform extraction and NaCl/EtOH precipitated with 20 μg glycogen carrier. The H3K4me3 in-situ ChIA-PET was performed according to the previously described protocol (Li et al., 2017) RNA libraries were prepared for sequencing by Novogene (CA). ChIP-seq library was prepared for sequencing according to the amplification protocol from Illumina.