Whole genome sequencing of coral reef: seawater
The libraries were normalized and pooled and then denatured in 0.05N NaOH and neutralized using HT1 buffer. The pool was loaded at 225pM on a Illumina NovaSeq S2 lane using Xp protocol as per the manufacturers recommendations. The run was performed for 2x150 cycles (paired-end mode). A phiX library was used as a control and mixed with libraries at 1% level. Base calling was performed with RTA v3. Program bcl2fastq2 v2.20 was then used to demultiplex samples and generate fastq reads.