RNA-Seq of mus musculus:E14.5 Tooth germ mesenchymal cells
In brief, the total RNA was extracted with Trizol(Invitrogen) according to the manufacturers instructions, mRNA was then purified and further reverse transcribed, labeled and am-plified to generate sequencing-ready cDNA library with TruSeq RNA Sample Prep Kit (Illumina, USA). The cDNA library concentration was determined with Qubit dsDNA HS Assay kit (Vazyme). The cluster generation and sequencing were performed on Illumina NovaSeq 6000  platform.