RNA-seq of human naive CD4+ Tcells: antagonist
In brief, the library was constructed using EASY-RNAseq method (Zhou et al., 2019) and then sequenced on Illumina HiSeq platform using the 150-bp pair-end configuration. After parsing through Trimmomatic for quality filtration, raw reads were mapped to the human genome reference GRCh38.p7. Counts were converted to FPKM values for normalization.