GSM5444459: Breast tumor 2, stiff gel, rep3 [H12805]; Homo sapiens; RNA-Seq
RNA was isolated using TRIzol (Invitrogen, Cat. #: 15596018) followed by chloroform extraction. RNAseq library preparation and sequencing was performed by the Vincent J. Coates Genomics Sequencing Laboratory, University of California, Berkeley. Samples were sequenced in NovaSeq 6000 S4 lanes with 150bp paired-end reads with approximately 25 million read pairs per sample.