GSM5140551: paracarcinoma(papillary thyroid cancer) tissues [N5]; Homo sapiens; ncRNA-Seq
RNA extraction method: Trizol The first step is to build a circular RNA library and remove ribosomes, using RiboMinus Human/Mouse Transcriptome Isolation Kit (invitrogen, thermofisher); the second step is to remove linear RNA, using RNase R enzyme (epicentre, illumina), and the third step is to construct Library, using KAPA Stranded mRNA-Seq Kit (roche)