GSM5284787: PA16 PDX tumors- vehicle control; Homo sapiens; RNA-Seq
Total RNA was isolated from snap frozen tumor tissue specimens using TRIzol-chloroform extraction. RNA was processed for sequencing using the RNeasy Mini Kit (Qiagen, Germantown, MA, USA). Purified RNA concentration and quality were determined by ND-1000 spectrophotometer using NanoDrop 3.0.1 software. RNA libraries were prepared for sequencing using standard Illumina protocol.