GSM5328705: ZI254,stress,non-deletion,replicate 2 [ZI254s_J2]; Drosophila melanogaster; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) following the manufacturer's protocol. Poly-A mRNA purification, reverse transcription, and high-throughput sequencing were performed by Novogene (Cambridge, UK) using a Novaseq 6000 sequencer (Illumina, San Diego, CA, USA) to generate 150-bp paired-end reads.