RNAseq of mouse skin epidermal stem cells wound edge
K14Cre; STat3fl/fl mice or control littermates were wounded with 6mm punch biopsy tools, 1 day or 6 days later, wounds or skin from unwounded region are excised and treated with trypsin to isolate epidermal keratinocytes.  Live, single epidermal stem cells were sorted based on markers (CD140a-CD31-CD117-CD45-Itga6HiCD34-Sca1+DAPI-) directly into TRIZOL.  Total RNA (10-30ng)was isolated and submitted for RNAseq.