16S survey freshwater lake
Primers for paired-end 16s community sequencing on the Illumina HiSeq platform using bacteria/archaeal primers 515F/806R. Sequencing requires use of 16S and Index sequencing primers, constructs below. Read 1 sequencing primer: Field description(space-delimited): 1, Forward primer pad 2, Forward primer linker 3, Forward primer  TATGGTAATT GT GTGYCAGCMGCCGCGGTAA Read 2 sequencing primer: Field description (space-delimited): 1, Reverse primer pad 2, Reverse primer linker 3, Reverse primer AGTCAGTCAG CC GGACTACNVGGGTWTCTAAT Index sequencing primer: AATGATACGGCGACCACCGAGATCTACACGC