RNA-Seq of Sphaerolaimus sp.
Total RNA was extracted using the Ambion RNAqueous Micro Kit following the manufacturer's protocol. Subsequent library preparation and cDNA synthesis was performed using the Takara SMART-Seq HT kit following the manufacturer's instructions. The resulting double-stranded cDNA was purified using Ampure XP magnetic beads. Final sequencing library preparation was performed using the Illumina Nextera XT kit.