GSM5770018: LDH KO3; Mus musculus; RNA-Seq
Cells were lysed in Trizol.  RNA was extracted by BGI, and quality was checked before sequencing Libraries were made BGI's platform. BGI-seq library construction method: mRNA molecules were purified from total RNA using oligo(dT) attached magnetic beads. mRNA molecules were fragmented into small pieces using fragmentation reagent. First and Second Strand cDNA synthesis. Prepare the end repair & add "A", adaptor ligation and PCR.