Illumina RNAseq of Hydractinia symbiolongicarpus 24h larvae
RNA-Seq libraries were constructed from 1 g RNA using the Illumina TruSeq RNA Sample Prep Kit, version 2.  The resulting cDNA was fragmented using a Covaris E210 focused ultrasonicator. Library amplification was performed using 10 cycles to minimize the risk of over-amplification. Unique barcode adapters were applied to each library.