RNA-Seq of Anabrus simplex: Adult female thorax
Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. In order to select cDNA fragments of preferentially 250~300 bp in length, the library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Subsequently, the library preparations were sequenced on an Illumina HiSeq4000 platform and paired-end reads were generated. After quality control and data filtering, these raw reads were pooled together to assemble a de novo transcriptome using Trinity with min_kmer_cov set to 2 by default and all other parameters set default. The reads were then filtered and clustered by Corset (v1.05) to remove redundancies and the longest transcripts per cluster were selected as unigenes.