RNA-Seq of Tetranychus kanzawai adult whole body
Sample preservation, RNA extraction, sequencing and assembly have been conducted for T. kanzawai, and P. citri based on methods previously described for spiders [18], with some modification. Briefly, a single specimen of each of the spider mite was preserved in RNAlater stored at -80 C, and RNA was extracted using Direct-zol RNA Microprep Kit (Zymo Research). Illumina library was prepared using KAPA HyperPlus Kit (KAPA Biosystems) targeting 300bp fragments. Sequence library was then sequenced on NextSeq 500 (Illumina) with High Output Mode 300 cycles as paired-end reads. Sequences were basecalled, demultiplexed, and adaptors sequences were removed with bcls2fastq v.2 software (Illumina).