Illumina NovaSeq 6000 paired end sequencing; Integrated longitudinal immunophenotyping, transcriptional and repertoire analyses depict principles of protective immune responses and memory formation in COVID-19 patients
Integrated longitudinal immunophenotyping, transcriptional and repertoire analyses depict principles of protective immune responses and memory formation in COVID-19 patients
Peripheral Blood Mononuclear Cells (PBMCs) were isolated by density-gradient centrifugation using Ficoll-Plaque protocol and cryopreserved for subsequent scRNA-Seq analyses Single cell suspension was used according to Chromium Next GEM Single cell V(D)J Reagent kits v1.1  Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005 Chromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns PN-1000016 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213  Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1, 16 rxns PN-1000165 Chromium Single Cell 5' Library Construction Kit, 16 rxns PN-1000020 Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Single Index Kit T Set A, 96 rxns PN-1000213