Partial 26S rRNA amplified from soil from Dung only and natural beetle infestation treatment on reclaimed post-coal mining site
The hypervariable V4 region of 16S rRNA library was constructed by following Illumina MiSeq protocol. Briefly, the protocol included a first stage amplicon PCR step involving 0.2 M concentrations of primers 515F (forward) and 806R (reverse). Sequences were demultiplexed and depleted of Illumina barcodes and primers by using MiSeq reporter