GSM5530962: Adherent monolayer cells_experiment #1; Homo sapiens; RNA-Seq
For total RNA extraction the RNeasy Kit (Qiagen) was used. Total RNA concentration and purity was measured with NanoDropTM 2000. RNA-sequencing libraries were prepared with the illumina TruSeq RNA v2 kit using 1 μg of total RNA. The libraries were checked with the Agilent bioanalyzer (DNA1000 chip), quantitated with the qubit HS spectrophotometric method and pooled in equimolar amounts for sequencing. Approximately 25 million, 75 bp long, single-end reads were generated for each sample on an Illumina NextSeq500 sequenc-er.