RNA-seq of mytilus californianus: adult gill
RNA was extracted from 0.04 g of gill tissue by grinding it in a mortar/pestle in liquid nitrogen with TRI reagent (Sigma), quantified by UV-Vis spectroscopy (Implen Nanophotometer), and cleaned with the Qiagen RNeasy Mini Plus Kit following the manufacturers protocol (Qiagen, MD, USA). Small fragment cDNA library construction, barcoding, and 150 bp paired-end (PE150; 12 samples used to generate the de novo transcriptome) or 50 bp paired-end (PE50; 49 samples from 5 treatments used to quantify expression levels) Illumina HiSeq 2500 rapid-run sequencing was performed by the Genome and Cytometry Core at the University of Southern California. The average fragment length and insert sizes were 350bp (PE150) and 380bp (PE50).