GSM4977273: DG-input 2; Mus musculus; ChIP-Seq
Lysates were clarified from sonicated PFA-fixed nuclei, and Twin-strep-tagged RORa-DNA complexes were isolated with Strep-Tactin. ChIP-Seq libraries were prepared with the SMARTer® ThruPLEX® DNA-Seq Kit (Takara, R400674) following the manufacturer's protocols.