GSM5597162: TNF 2; Mus musculus; RNA-Seq
Primary hepatocytes were harvested in Trizol using a Tissuelyzer II (Qiagen) and RNA purification was performed using EconoSpin columns (Epoch). A total amount of 500 ng of total RNA was used and sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. Prepared PCR products were purified and size-selected on AMPure XP beads (Beckman A63881), and library quality was assessed on the Agilent Bioanalyzer 2100 system. The prepared libraries were sequenced (paired-end) on a Illumina platform.