GSM5283810: RNAseq Luc KD Veh rep2; Homo sapiens; RNA-Seq
MCF-7 cells with shRNA-mediated knockdown of luciferase (Luc; as a control) or PARP-1 were isolated and subjected to RNA-seq as described previously (PMID: 21807852, PMID: 26236012). Estrogen-withdrawn MCF-7 cells were treated with ethanol or 100 nM E2 for 3 hours. Total RNA was isolated MCF-7 cells using the RNeasy kit (Qiagen, 74136) according to the manufacturer's instructions.  The RNA collected was processed for whole transcriptome polyadenylated RNA sequencing (polyA+ RNA-seq).