GSM4976913: ATAC-seq 24h 9/26/17; Drosophila melanogaster; ATAC-seq
Upon dissociation, GFP-labeled L3 neurons were FACS sorted directly into 50 μL of lysis buffer (10 mM Tris-HCl ph7.4, 10 mM NaCl, 3 mM MgCl2 and 0.1% IGEPAL CA-630). Immediately after lysis, the nuclei were spun down at 800 g for 10 min at 4°C and the supernatant was carefully removed. The pellet was resuspended in the transposase reaction mix (25 μL of 2×TD buffer [Illumina Nextera DNA Library Prep kit], 2.5 μL of Transposase [Illumina Nextera DNA Library Prep kit] and 22.5 μL of nuclease-free water) and incubated at 37°C for 30 min. Following the transposition, the DNA sample was purified with Qiagen MinElute kit. DNA libraries were constructed using Illumina Nextera protocols