GSM5769554: ChIP_H3K9me2_nrpd1-kd_Rep4; Oryza sativa; ChIP-Seq
Sheared chromatin from the equally developed pre emerged panicle of WT and kd plants were incubated with appropriate antibodies bound to Protein G Dynabeads (Thermo Fisher, catalog number 10003D).  Washes, elution, de-crosslinking and DNA purification was done as described. The purified DNA was taken for library preparation. The ChIP library was constructed using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (Catalog no-E7103L) as per manufacturer's instructions. The adapter sequence used is AGATCGGAAGAGCACACGTCTGAACTCCAGTCA. The sequencing was done in single end mode in a Illumina HiSeq2500 platform.