C3_178_NB12_3 CX stands for culture cycle number X, it is followed by the time post innitial innoculation start in hours, then the concentration in ng/ml (0.01, 1, 100, NB12 - for no B12) in minimal salts medium, and the sample replicate number
PCR amplification and product indexing, folllowed by pooling with the rest PCR products, Illumina adapter ligation and library prep with the TruSeq DNA PCR free and demultiplexing to the original product using 0 mismatch index identification