GSM5211800: ARID1B_CTRL_LINE1_rep2; Homo sapiens; ChIP-Seq
RNA-seq Cells were lysed in Tri-reagent (Zymo research) and total RNA was extracted using Quick-RNA Miniprep kit (Zymo research). RNA libraries were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module, NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer's instructions (New England Biolabs). 1 μg of total RNA input was used for each library ATAC-seq: 50000 cells per condition were processed as described in the original ATAC-seq protocol paper (Buenrostro et al 2013) ChIP-seq: Barcoded libraries were made with NEB ULTRA II DNA Library Prep Kit for Illumina, and sequenced on Illumina NextSeq 500, producing 75bp SE reads.