Genome re-sequencing of E3-64
Sample testing included concentration, sample integrity and purity. Concentration was detected byfluorometer or Microplate Reader (e.g. Qubit Fluorometer, Invitrogen). Sample integrity and purity were detected by Agarose Gel Electrophoresis (Concentration of Agarose Gel:1% Voltage:150V,Electrophoresis Time:40 min).1g genomic DNA was randomly fragmented by Covaris.The fragmentedgenomic DNA were selected by Agencourt AMPure XP-Medium kit to anaverage size of 200-400bp.Fragments were end repaired and then 3 adenylated. Adaptors were ligated to the ends of these 3adenylated fragments.This process was to amplify fragments with adaptors from previous step. PCRproducts were purified by the Agencourt AMPure XP-Medium kit.The double stranded PCR products wereheat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA)were formatted as the final library.Library was qualified by QC.The qualified libraries were sequenced byBGISEQ-500: ssCir DNA molecule formed a DNA nanoball (DNB) containing more than 300 copiesthrough rolling-cycle replication. The DNBs were loaded into the patterned nanoarray by using high densityDNA nanochip technology. Finally, pair-end 100 bp reads were obtained by combinatorial Probe-AnchorSynthesis (cPAS).