GSM5990056: circ-45; Zea mays; RNA-Seq
Three replicates were prepared per tissue. RNA was extracted from 5–10 individual plants per replicate. The three replicates were extracted separately and then mixed in equal amounts for sequencing. Total RNA was isolated from 1cm ear using a Direct-zol RNA Microprep kit (Zymo Research) following the manufacturer's guidelines. RNA libraries were prepared for sequencing using corresponding standard protocols.