EDF_T2_16
Total soil DNA was extracted from 0.25 g lyophilized soil using the Qiagen DNeasy PowerSoil kit and checked for quality (260:280 > 1.75; 260:230  > 1.70) on a spectrophotometer (DS-11 FX+, DeNovix, Wilmington, DE, USA). Quantity of DNA per sample was determined using Invitrogen PicoGreen Quanti-IT dsDNA assay kits (ThermoFisher Scientific, Waltham, MA, USA). Equimolar equivalents of DNA were used as templates for preparation of 16S rRNA amplicon libraries. The prokaryotic V4 region of 16S rRNA genes were targeted using dual barcoded primers (515F/806R) (Kozich et al., 2013)  Reactions utilized Platinum Hot Start high fidelity PCR master mix (ThermoFisher Scientific, Waltham, MA, USA), and were pooled in equimolar concentrations prior to running 2 x 250 bp paired-end sequencing on the Illumina MiSeq platform. All 16S sequencing was carried out by Microbiome Insights (Vancouver, BC, Canada).