low-yield-penalty(LPB)_vegetative-stage_field-dehydration-condition
Total RNA was extracted from frozen flag leaves using the miRVana miRNA isolation kit according to manufacturers protocol (Invitrogen, Carlsbad, CA). RNA from three individual plants in each genotype were pooled to create a composite sample representing each replicate. RNA-seq libraries were constructed from the pooled RNA across genotypes, developmental stages and and treatments and sequenced with two technical replicates (A and B) in the Illumina HiSeq3000 (Oklahoma Medical Research Foundation, Norman, OK).