GSM6294314: OTII_day1_biol_rep_2; Mus musculus; RNA-Seq
Sorted cells were snap frozen and then RNA was extracted with the RNeasy Mini Kit (QIAGEN). RNA was checked by gel electrophoresis and quantified with a Qubit Fluorometer (Invitrogen). Barcoded sequencing libraries were constructed using the TruSeq Stranded mRNA kit (Illumina).