RNA-Seq of Serratia sp. strain EWG9: {Minimal medium grown (sole nitrogen source: potassium nitrate)}
RNAseq analysis was performed to evaluate the differences in gene expression patterns between bacterial strain EWG9 cells grown in minimal salt media using two different sole nitrogen sources. In this study, Serratia sp. strain EWG9 cells were grown in minimal salt medium (using potassium nitrate as sole nitrogen source), for 5h before harvesting. Total RNA was isolated from bacterial samples using conventional TRIzol method with minor modifications, quality and quantity checked on 1% denaturing RNA agarose gel and NanoDrop respectively. The RNA-Seq paired end sequencing libraries were prepared from QC passed RNA samples using MICROBExpress kit and Illumina TruSeq Stranded mRNA sample Prep kit. After obtaining Qubit concentration for the libraries (mean fragment distribution length of 350 bp), the PE Illumina sequencing libraries were loaded onto Illumina platform for cluster generation and sequencing.