GSM5830975: TPC2-4 TNFα+JNKi; Homo sapiens; ATAC-seq
For ATAC-seq, 50,000 TPC2-4 cells were precipitated and subsequently re-suspended with 50 μl cold lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) igepal CA-630) and incubated on ice for 10 minutes (min). After centrifuge at 500 g for 10 min at 4℃, the nuclei were re-suspended with 100 μl wash buffer (10 mM Tris-HCl pH7.5, 10 mM NaCl, 3 mM MgCl2). Followed by washing twice, the transposable reaction and DNA library preparation was performed accordingly with TruePrep DNA Library Prep Kit V2 (TD501-01, Vazyme). The successfully prepared libraries were sequenced on the Illumina PE150 platform (Novogene). Library preparation was performed accordingly with TruePrep DNA Library Prep Kit V2 (TD501-01, Vazyme). The successfully prepared libraries were sequenced on the Illumina PE150 platform (Novogene).