Small-RNA Sequencing of locusts
The crude virus extract was inoculated with locusts, and then the locusts were collected to extract total RNA.Use the Small RNA Sample Pre Kit to construct the library, take advantage of the special structure of the 3' and 5' ends of Small RNA (complete phosphate group at the 5' end and hydroxyl group at the 3' end), and use total RNA as the starting sample to directly transfer Small RNA. Adapters are added at both ends of the RNA, and then reverse transcribed to synthesize cDNA. After PCR amplification, PAGE gel electrophoresis separates the target DNA fragments, and the recovered cDNA library is obtained by cutting the gel.