Complete genome sequence of Acinetobacter sp. Y-23
For Pacific Biosciences sequencing, 20k insert whole genome shotgun libraries were generated and sequenced on a Pacific Biosciences RS instrument using standard methods. An aliquot of 8g DNA was spun in a Covaris g-TUBE (Covaris, MA) at 6,000 RPM for 60 seconds using an Eppendorf 5424 centrifuge (Eppendorf, NY). DNA fragments were then purified, end-repaired and ligated with SMRTbell sequencing adapters following manufacturers recommendations (Pacific Biosciences, CA). Resulting sequencing libraries were purified three times using 0.45 x volumes of Agencourt AMPure XPbeads (Beckman Coulter Genomics, MA) following the manufactures recommendations