GSM6257914: CONTROL, RECOVERY, REP2; Escherichia coli; RNA-Seq
RNA was stablized using RNAprotect bacteria reagent (Qiagen, Germantown, MD). Total RNA was extracted using RNeasy Mini Kit with on-column DNA digestion with DNase I (Qiagen, Germantown, MD) following manufacturer's instruction. rRNA depletion was performed using RiboCop rRNA Depletion Kit (Lexogen, Greenland, NH). RNA libraryies was prepared using PrepX RNA-seq library preparation kit (Takara Bio, San Jose, CA).