AS96hT2
Six experimental SPF Beagle dogs from the same litter, 6-7 weeks old, were purchased from and housed at the National Canine Laboratory Animal Resource Center (Guangzhou, China), and were divided into experimental and control groups. After a week of breeding to adapt to the laboratory environment, the puppies in the experimental group were infected with 300 infectious eggs and puppies in the control groups were injected with equal amounts of normal saline. At 96 hpi, blood was collected from the jugular vein of six puppies and was placed into tubes with heparin. Then PBMCs were separated from blood using Dogs PBMCs Isolate kit (TBDscience, Tianjin, China). Subsequently, PBMCs were sent to Beijing Novogene Technology Co., Ltd. for RNA sequencing utilizing the Illumina Novaseq 6001 Platform.