GSM5830885: YTHDF3_RIP_rep3; Mus musculus; RIP-Seq
For each replicate, approximately 1.5x106 cultured neurons growing in a 6 well plates were used. Cells lysates (25mM Tris-Cl pH7.4; 150mM NaCl; 2.5mM EDTA, 0.5mM DTT; 0.5% NP-50) were used for RIP using antibodies targeting the endogenous YTHDF1, YTHDF2 or YTHDF3 antibody. Total RNA was extracted from a fraction of the cell lysate (Input samples) or eluted from antibody coupled beads using 1mL of TRIZOL reagent (Thermo) and the RNA was extracts according to the manufacturer's protocol. RNA was resuspended in Nuclease-Free water. 9ng of total RNA was used for library preparation with the SMARTer Stranded Total RNA-Seq Kit V3 (Takara).