scRNA-Seq of mus musculus:E14.5 Tooth germ mesenchymal cells were cultured 7 days at passage 1 in N2B27 media
Cell concentration was determined using a hemocytometer and adjusted to obtain the target concentration for the 10X Chromium chip loading. The cells were loaded into the 10X Chromium Controller, and the Single Cell 3 Reagent Kit v5 was applied according to the manufacturers protocol. Following library preparation and quantitation, the libraries were sequenced on the Illumina HiSeq X Ten platform.