RAD-seq of Pimelodus grosskopfii: Adult from Cauca river
Genomic DNA was extracted from ethanol-preserved muscle tissues by using the Qiagen DNeasy Blood & Tissue kit. At least 0.5 g of high-molecular-weight DNA was employed to prepare RAD libraries using a 6-bp restriction enzyme (PstI) for digestion, followed by sonication and adapters attaching. Finally, a single-end sequencing step was conducted by Floragenex Inc. (http://floragenex.com) on one lane of an Illumina HiSeq 2000 machine