GSM5870283: s, ESC, WT+MosIR, RS7.2; Mus musculus; ncRNA-Seq
Cells were washed with PBS, homogenized in Qiazol lysis reagent (Qiagen) and total RNA was isolated by Qiazol-chloroform extraction and ethanol precipitation method. RNA quality was verified by Agilent 2100 Bioanalyzer. Small RNA libraries were constructed using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer's protocol. Small RNA libraries were size selected on 6% PAAGE gel, a band of 140 - 150 bp was cut from the gel and RNA was extracted using Monarch® Genomic DNA Purification Kit. Quality of the libraries was assessed by Agilent 2100 bioanalyzer.