16S rRNA gene amplicon sequencing of snow from Zugspitze; 2016; 0-96cm [JMF-2006-1-0022A]
Snow samples taken along depth profiles of snowpacks were melted. Cells from 2 L snow meltwater were collected on 0.2 m polycarbonate membrane filers. DNA was extracted from the filters using bead beating and phenol-chloroform extraction as described by Pilloni et al. (PMID: 22808168).