GSM5597055: Bisulfite-seq_SID-2H; Mus musculus; Bisulfite-Seq
For DNA methylation sequencing, genomic DNA from the livers of C57B/6J mice was extracted and purified using the Qiagen DNeasy Kit; For RNA sequencing, mice livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. CpG methylation status across the genome was characterized using methylation-sensitive HpaII and the methylation-insensitive isoschizomer MspI; RNA-seq libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina).