RNA-Seq of Gallus gallus: adult male Liver
We used liver samples from two chicken breeds, including Ross 708 as a commercial breed with low RFI and Isfahan chicken as a local breed with high RFI. Then 12 g of their liver tissues were excised from similar parts of each bird. The collected samples were immediately put in liquid nitrogen and then stored at 80 C for further use (RNA  sequencing). Three RNA samples from the same breed were pooled (by mixing together equal quantities of RNA) to generate a total of 4 pooled RNA samples (two samples in each breed). All the samples were subjected to a paired-end sequencing of 2 * 150 bp on the Illumina HiSeq 2000 platform (Illumina, San Diego, California, USA).