Influence of heavy metals on wild Peromyscus gossypinus
All files have already been de-multiplexed. PCR libraries for bacterial 16S were generated using the S-D-Bact-041-b-S-17 (5-CCTACGGGNGGCWGCAG-3) forward and S-D-Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC-3) reverse primer pair, which amplify an approximately 444 bp fragment (Klindworth et al., 2013). Modifications to the primer sets (fusions: 8 forward + 12 reverse) were done according to TaggiMatrix protocol which included fusions with universal 5 iTru sequences containing up to 96 dual combinations of unique internal tags (Glenn, Pierson, et al., 2019). DNA from each sample was PCR amplified using the 16S-iTru fusions in 25 L reactions using the KAPA HiFi Hotstart PCR kit (KAPA Biosystems, Wilmington, MA, 01887, USA).