RNAseq of K18-hACE2-mouse lung
Lungs from mice were homogenized using gentle MACS dissociator and TRI reagent was added to the homogenate. RNA was purified from TRI reagent using Direct-zol RNA miniprep. RNA was quantified by Qubit and TapeStation. RNAseq libraries were prepared with KAPA RNA HyperPrep Kit with RiboErase for RNAseq analysis.