RNA-Seq of Glycine max flower: stage 2
For Gene Expression Profiling (GEx) library construction, QuantSeq 3mRNA-Seq Library PrepKit FWD for Illumina (Lexogen GmbH, Wien, 510 Austria) was applied according to themanufacturer's protocol. Libraries were diluted to 1.8 pM for 1x87 bp single-end sequencing with75-cycle High Output v2 Kit on the NextSeq 550 Sequencing System (Illumina, San Diego, CA,USA) according to the manufacturers protocol. QuantSeq FWD allows to exactly pinpoint the 3end of poly(A) RNA and therefore obtain accurate information about the 3 UTR. Using thissequencing fragments of coding sequences are 260-300 bp long on average.