VAA_B3
The TruSeq library preparations were constructed using the cDNA Synthesis kit (Illumina Inc., San Diego, CA, USA). Single-end (150 bp) sequencing of the cDNA libraries were performed on the Illumina HiSeq 2000 (Illumina Inc., San Diego, CA, USA). Library preparation and sequencing were performed by AgriLife Genomics and Bioinformatics Services (Texas A&M University, College Station, TX, USA). Sequence cluster identification, quality prefiltering, base calling and uncertainty assessment were done in real-time using Illumina's HCS 2.2.58 and RTA 1.18.64 software with default parameter settings. All the reactions followed the respective manufacturers instructions.