negative control (COI)
environmental DNA (eDNA), COI was PCR amplified for each replicate seperately (12S rRNA was also amplified, but not utilized, should not be included here); primers were BF1 (ACWGGWTGRACWGTNTAYCC) and BR2 (TCDGGRTGNCCRAARAAYCA; (Elbrecht and Leese 2017); inline barcodes were removed during demultiplexing, but each read starts with  6 random basepairs to remove PCR duplicates (before primer region)