GSM6257589: IL6 KO scRNAseq; Mus musculus; RNA-Seq
Red blood cells from freshly collected total bone marrow cells using RBA lysis buffer. The remaining bone marrow cells were resuspended in PBS with 0.5% BSA. An estimated 10,000 cells were loaded into the 10X Chromium system per sample. RNA was converted to cDNA and libraries were generated using the Chromium Single Cell 3' v3 Kit. Bioanalyzer confirmed the main peak size of cDNA were generally between 450-490 bp.