GSM5966568: NPA_SMtrt_rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from cultured cells using the EuroGoldTriFast reagent (EuroClone) according to the manufacturer's instructions. RNA concentration was determined using a NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and RNA integrity was assessed on a LabChip GX Touch (PerkinElmer, Waltham, MA, USA). The Illumina TruSeq Stranded mRNA Sample LS Preparation Kit (Illumina, San Diego, CA, USA) was used with 500 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols