GSM5531314: H2O_H3K4me3_input1; Mus musculus; ChIP-Seq
The embryos were washed 3 times in 0.5% BSA after removal of zona pellucida. After cell lysis, the chromatin was digested with micrococcal nuclease and histone-DNA complexes were isolated with corresponding antibodies. Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645).