MPS5
Metagenomic DNA was extracted from mouse fecal samples, checked for quality  evaluation.  Purified DNA was  amplified for 16S rRNA hyper variable region V3-V4using KAPA HiFiHotStart Ready Mix and modified341F and 785R primers  and sequence libraries were prepared. Raw sequence data was filtered using Trimgalore, checked for chimeric sequences and sequenced on Illumina platform