GSM5904910: E14 Day 6 mPGCLC rep2; Mus musculus; ncRNA-Seq
mPGCLCs were isolated from Day 6 trypsin-dossiciated Embryoid Bodies using reporter gating (Stella-GFP positive and Esg1-tdTomato negative) via FACS and sorted directly into Qiazol. Small RNA fractions was isolated using the Qiagen miRNAeasy-micro kit. Small RNA libraries were made using the Perkin Elmer NextFlex small RNA Kit v3, following the instructions for low input.These contain 4 random Ns at the adapter ends. small RNA seq (Single Read 50)