seeds 18 days after flowering, replicate 3
In brief, poly(A) mRNAs were enriched and pulled down by the magnetic oligo (dT) beads and fragmented into small pieces (~ 200 bp). Then, random hexamer (N6) primers were used to construct the double strand cDNA libraries. After the end repair by phosphate at 5-end and sticky A at 3-end, the cDNA libraries were ligated with sequencing primers to build the final RNA-Seq libraries.