Fucus vesiculosus male 3 vegetative tissue
Sequencing libraries were prepared from 1g RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs). The RNA was incubated for fragmentation for 6 minutes . No size selection was performed. The libraries were sequenced twice on the Illumina NextSeq 500 (150-bp pair-end reads), using the NextSeq 500/550 High Output Kit v2.5 (300 Cycles).