RNA-Seq of Dromaius novaehollandiae: adult fat
Each fat sample (a pool of six biopsies from different spots of the back and abdominal fat pads) was homogenized and prepared for RNA-sequencing. Illumina Mi-Seq system was used to sequence the emu fat transcriptome library. Raw reads were filtered with Q20 quality trimming to remove low quality reads with average quality score <20 and trimming of low-quality bases from the end of reads. Sequence pre-processing software Trimmomatic (v0.30), was used to obtain clean paired-end and single-end Mi-Seq data.