GSM5494073: scRNA.CTRL; Mus musculus; RNA-Seq
Pancreata were harvested from C57BL/6J wild-type mice at different time points before and after acute inflammation and digested to obtain single cell suspension. After depletion of CD45-positive cells, primary cells derived from 3 pancreata were mixed for each time point, and 10,000 cells were captured followed by library preparation using 10X Chromium single cell 3' V3 chemistry according to manufacturer's protocol. Libraries were then multiplexed and sequenced on a single lane of NovaSeq6000 S2‐100 flow cell (Illumina).