RNAseq of FLP-in CHO-K1 cells: MTIV Construct 4h on Val-free media rep 2
RNA was extracted from cells using the Qiagen RNeasy Kit (Qiagen, 74104) according to the manufacturers protocol. QIAshredder homogenizer columns were used to disrupt the cell lysates (Qiagen, 79654). mRNA was purified using the NEBNext poly(A) mRNA Magnetic Isolation module (New England Biolabs, E7490) in accordance with the manufacturers protocol. Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, E7770), and sequenced on a NextSeq 550 single-end 75 cycles high output 342 with v2.5 chemistry. _