Kpn 132_trimethoprim_R1, Kpn 132_trimethoprim_R2
Two-step PCR was used to construct the sequencing library, with two sub-libraries mixed. Kpn 132_trimethoprim_R1: Barcode_TCGCCTTA; Kpn 132_trimethoprim_R2: Barcode_CTAGTACG.