RNA-seq of populus tomentosa leaves from overexpression transgenic plant treated with methyl viologen
Total RNA was isolated from WT, OX and anti untreated or treated with MV using TRIzol reagent according to the manufacturers protocol (Invitrogen). The samples were sequenced using a Illumina Genome Analyzer (HiSeq 2000; Illumina, San Diego, CA). The raw reads were data-filtered to obtain high-quality clean reads by SOAPnoke, and mapped to the P. trichocarpa reference genome and reference genes using HISAT. Gene expression levels were calculated as expected number of Fragments Per Kilobase of transcript sequence per Millions base pairs sequenced (FPKM)