Meta-transcriptome of Pocillopora acuta: HTAC-6weeks-1302
Nubbins were collected from 450 Pocillopora acuta colonies between 4-10 September 2018. They were fixed using hot glue to individual coral fragment mounting plugs and allowed to acclimate for roughtly 14 days before they were randomly allocated to one of four experimental conditions: Ambient Temperature Ambient pCO2 (25.95C and 7.99 pH), Ambient Temperature High pCO2 (25.93C and 7.8 pH), High Temperature Ambient pCO2 (29.5C and 7.97 pH), and High Temperature High pCO2 (29.5C and 7.78 pH) for two months (22 September - 17 November 2018), and then to Ambient Temperature Ambient pCO2 (25.95C and 7.99 pH) condition for a two month recovery period (18 November 2018 - 12 January 2019). Temperature levels at the beguining of the recovery period were decreased over the course of a week to ambient levels. Sampled fragments were placed in sterile whirlpak bags and snap frozen in liquid nitrogen at 13:00 each day (with the exception of 6, 12 and 30 hr time points) and stored at -80C. This Pocillopora acuta sample was from the High Temperature Ambient pCO2 condition and was collected after 6 weeks of treatment on the 03-Nov-2018. Sample Plug ID: 1302