Amplicon sequencing of the Arbuscular Mycrrhizal Fungi SSU 18S rRNA Rhizosphere
The intraradical AMF communities were analysed via Illumina MiSeq 2x300 bp pair-end sequencing, using a semi-nested approach targeting the SSU region of the 18S rRNA gene. The Glomeromycota-specific AML1-AML2 primer pair (Lee et al., 2008) was used initially, followed by WANDA-AML2 (Dumbrell et al., 2011).