GSM5905355: RNAseq_B6_Chow_6 [C_B6_61_4]; Mus musculus; RNA-Seq
Total RNA from mouse liver tissue was extracted using the miRNeasy Mini Kit (QIAGEN) and QIAzol Lysis Reagent (QIAGEN) according to the manufacturer's protocol. Extracted RNA was treated with DNase using RNase-Free DNase (QIAGEN). Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit (New England BioLabs). Libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer's instructions.