Nanopore long read cDNA-PCR sequencing of recombinant 3' end
Library prepared using total RNA extracted from U. maydis cells expressing a recombinant chimeric UmaTR construct. Total RNA was G/I tailed using the polyA length kit (USB). This was followed by reverse transcription using an oligo-dC reverse primer containing a 5' nanopore compatible adapter. cDNA was amplified using 1 round of nested PCR to enrich for recombinant UmaTR sequences. SQK-PCS109 PCR-cDNA library preparation kit instructions largely followed with above modifications. Subsequent steps followed according to kit. Raw reads prior to processing deposited in this entry