GSM4977699: RNA MSIT235; Homo sapiens; RNA-Seq
Human innate lymphoid cells (ILCs) were sorted from peripheral blood mononuclear cells using BD FACSAria instrument. Once sorted, ILCs were processed for single cell analysis using 10x Genomics kit (3' v3 chemistry). For gene expression libraries (GEX), 50 ng of amplified cDNA was used for library preparation, consisting of fragmentation, end repair, A-tailing, adapter ligation and sample index PCR as per manufacturer's instructions. Libraries were sequenced on a HiSeq 4000 flow cells, targetting 25,000 read pairs/cell. For cell surface protein (CSP) libraries, 2 uL of amplified cDNA was used for library prepartion, consisting of fragmentation, end repair, A-tailing, adapter ligation and sample index PCR as per manufacturer's instructions. Enriched libraries were sequenced on a HiSeq 4000 flow cell, targetting 5,000 read pairs/cell. RNA and protein libraries were generated using 10x genomics protocol (3' v3 chemistry).