GSM5212161: AGO2_Mock_bioreplicate_1; Arabidopsis thaliana; RIP-Seq
Total RNA was extracted using TRIzol reagent (Life Technologies) following the manufacturer instructions. Total RNA was directly used for sRNA library preparation. PARE sequencing was obtained by purification with the NEB mRNA isolation kit (New England Biolabs). sRNA libraries were produced using the TruSeq Small RNA Sample Preparation Kit (Illumina). PARE libraries were constructed according to Zhai et al (2014).