Vehicle_48h_IPSC_Rep4
Total RNA was isolated using the RNEasy Mini Kit (Qiagen). An on-column DNAse digestion was included.  350 ng total RNA per sample was used as input for library preparation. Ribosomal RNA was depleted using the Ribo-Zero Gold rRNA Removal Kit (Illumina). rRNAdepleted RNA was processed into sequencing libraries using the TruSeq Stranded Total RNA kit (Illumina). Paired-end sequencing reads were subsequently generated on a HiSeq4000 instrument.