RNA-seq of mus musculus:only pEGFP group
The eukaryotic mRNA was enriched with magnetic beads with Oligo (dT). Next,add fragmentation buffer to break mRNA into short fragments, use mRNA as template, synthesize the first cDNA strand with six-base random primers (random hexamers); then, add buffer, dNTPs, RNase H and DNA polymerase I to synthesize the second strand cDNA chain. After purification by QiaQuick PCR kit and elution with EB buffer, the end repair is done and the sequencing adapter is connected, then fragment size selection is performed by agarose gel electrophoresis, and finally the library is prepared by PCR amplification and enrichment of cDNA.