RNA-Seq of donkey: adult male visceral adipose
Rotal RNA was extracted from the samples using Trizol reagent. RNA-seq libraries were sequenced in a single lane using an Illumina Novaseq 6000 sequencer (Illumina, San Diego, CA) after being quantified by TBS380. Before de novo transcriptome assembly, quality control was conducted on the sequencing data using SeqPrep software (https://github.com/jstjohn/SeqPrep). After assembly, the results were re-evaluated. Contigs with chimera, incomplete assembly, and base errors were removed using TransRate (http://hibberdlab.com/transrate) and CD-HIT (http://weizhong-lab.org/cd-hit) software. The longest transcript from potential alternative splicing transcripts was defined as a unigene.