GSM6001738: WT fibroblast; Mus musculus; RNA-Seq
Mouse pithelial cell suspensions were generated accroding to protocol in (Vega et al., 2022). Cells were encapsulated using the inDrop platform (1CellBio), and RNA was extracted according to the protocol of Klein et al., 2015. Chen et al., 2021. CEL-Seq linear amplification of using standard structure (single index) (Klein et al., 2015) or TruDrop library structure (dual index) (Southard-Smith et al., 2020) RNA-Seq was performed on Novaseq6000 with a 2 x 150 paired-end kit standard run (for dual index) RNA-Seq was performed on BGI MGI-Seq (for single index) 100 read 1, 50 read2