GSM5870385: chronic ethanol + gavage 9 hour, rep1; Mus musculus; RNA-Seq
Livers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. mRNA sequencing libraries were constructed with KAPA mRNA HyperPrep Kits (KAPA Biosystems; Cat # KK8580) followed by manufacturer's protocol.