GSM6346352: RPE_CH; Homo sapiens; RNA-Seq
Retinas were dissected and digested to generate single cell suspension. Samples were immuno-labelled with BD single-cell multiplexing antibodies. Viable CD45+CD11B+ single cells were sorted using FACS. Tow libraies of RPE/choroid and neuroretinas were constructed using BD Rhapsody™ Single-Cell Analysis System with Whole Transcriptome Analysis (WTA) Amplification Kit.