GSM5831045: Tg_AngII_ATA_PVAT_3; Mus musculus; RNA-Seq
For WT mice, RNA samples were prepared by using the RNeasy® mini kit (Cat# 74106, Qiagen) according to the manufacturer's instruction. Each RNA sample was extracted from pooled PVAT from 2-3 mice. For mitoNEET-Tg mice, RNA samples (each sample represents single mouse) were isolated by using the RNeasy® micro kit (Cat# 74004, Qiagen). The RNA library was prepared with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) with poly A selection. This pool was subjected to 151 bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeq). An average of 40 million paired-reads per samples were generated.