GSM5904915: Retina Mll5-KO rep1; Mus musculus; RNA-Seq
For the preparation of total RNA for RNA-seq, 8 eyeballs of two pairs of female Mll5-WT and Mll5-KO mice at the age of P18 were dissected. Total RNA was extracted with RNeasy mini kit (Qiagen, 74104).  A minimum of 2 μg of total RNA obtained from mixture of 2 eyeballs were used for library construction RNA libraries were prepared for sequencing using standard Illumina protocols