16S rRNA amplicon sequencing of mixed methanotrophic community with dAMS medium
1.5 mL culture centrifuged, pellet frozen, DNA extracted using FastDNATM spin kit (MP Biomedicals, USA). Duplicate amplification of the 16S rRNA gene, primers Bacteria Bakt341F (5- CCTAYGGGRBGCASCAG-3) and Bakt805R (5-GGACTACNNGGGTATCTAAT-3) to amplify the V3-V4 hypervariable region