GSM5493635: Primary liver sinusoidal endothelial cells ( x3 ); Rattus norvegicus; RNA-Seq
Primary LSECs transcriptome profile under physiological or pathological hydrodynamic pressures was examined by mRNA sequencing. Briefly, mRNA was isolated using the RNeasy® Micro Kit (Qiagen, Hilden, Germany) following manufacturer's instructions. The sequencing library was prepared using 25 ng of total RNA by a Universal Plus mRNA-Seq NuGEN (0508, 9133, 9134, Tekan, Leek, The Netherlands). Single-end mRNA-seq was performed in the Illumina platform HiSeq2500