GSM5284448: Imida-R2; Aedes aegypti; RNA-Seq
Three pools of thirty 4th stage larvae from each line (Imida-R, Imida-S and Bora-Bora) not exposed to insecticide were used for RNA extractions using the RNAqueous-4PCR kit (Life technologies) according to manufacturer's instructions. Three RNA-seq libraries were prepared per line using the TruSeq Stranded mRNA sample Prep kit (Illumina) following manufacturer's instructions. After quality check, libraries were sequenced (paired reads) in multiplex on an Illumina GAIIx sequencer by Hybrigenics-Helixio (Clermont-Ferrand, France).