Pool-sequencing of genomic DNA from 31 Acipenser ruthenus males
DNA pool-seq libraries were prepared according to manufacturers protocols using the Illumina TruSeq Nano DNA HT Library Prep Kit (Illumina, California, USA). Briefly, from 200 ng of each sample, DNA was fragmented (550 bp) by sonication on a M220 Focused-ultrasonicator (COVARIS). Size selection was performed using SPB beads (kit beads) and 3-ends of the blunt fragments were mono-adenylated. Then, adaptors and indexes were ligated and the construction amplified with Illumina-specific primers (8 cycles). Library-quality was assessed using Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification Kit (Roche).