fungal community from lung tissue of Peromyscus crinitus
ExTaq polymerase PCR with ITS1-F and ITS4 primers followed by a second PCR with linkers, barcode, and ITS2 5.8S-FUN and ITS4-FUN primers. Purified with Ampure XP beads. DNA libraries following the Illumina MiSeq DNA library preparation protocol for paired-end reads. Sequencing on MiSeq Illumina PE300.