GSM6215767: TET2 TT Donor ATAC processed genomic DNA; Homo sapiens; ATAC-seq
On Day 5 40,000 cells were harvested, and bulk Omni-ATAC was performed Cells were lysed with ATAC-Resuspension Buffer containing 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin for 3 minutes, and then the transposition was performed for 30 minutes at 37 C using 100 nM of Illumina Tagment DNA TDE1 Enzyme and Buffer Kit per 50,000 cells. The fragmented DNA was then cleaned up using a Zymo DNA Clean and Concentrator-5 Kit (cat# D4014). The transposed fragments were amplified and indexed using NEBNext 2x Master Mix. The final PCR product was purified using the Zymo DNA Clean and Concentrator-5 Kit.