RNA-Seq of mus musculus: adult male liver
Male C57BL/KS-db/db and db/+ mice (5-6 weeks) were adapted for 1 week, and randomly divided into groups. The db/+ mice in Control and db/db mice in Model were treated with water, and db/db mice in CTF and MET with CTF and MET by gavage for 15 consecutive weeks, respectively. Mice were anesthetized and sacrificed. Liver was flash frozen. Total RNA was extracted, and sequencing. Raw reads were processed for clean reads, aligned to the reference genome, and mapped reads were counted.