megenome of Leontynka pallida enriched culture (bacteria present)
Nanopore sequencing was performed using 4 g of genomic DNA. The DNA was sheared at 20 kbp using Covaris g-TUBE (Covaris) according to the manufacturers protocol. After shearing, two libraries were prepared using Ligation Sequencing Kit from Oxford Nanopore Technologies (SQK-LSK108). The prepared library was loaded onto a R9.4.1 Spot-On Flow cell (FLO-MIN106). Sequencing was performed on a MinION Mk1B machine for 48 hours using the MinKNOW 2.0 software.