WGS of Seneca strain Lake Trout
The library was prepared using 100ng of input DNA and the NEBNext Ultra Library Preparation Kit for Illumina. The library was sheared to approximately 400 bp using a Covaris M220 Ultrasonicator, amplified for eight cycles, and quantified using Quant-It Picogreen dsDNA assays run in triplicate. Fragment size was assessed using a genomic DNA Tape Station assay before sequencing on a HiSeqX instrument with 2X150 bp paired end reads.