Horn_3
Total RNA was extracted from 80mg of liver tissue using TRI Reagent (Ambion), according to the manufacturers recommendations. An additional DNAse treatment using DNASE RQ1 (Promega) was included to eliminate traces of genomic DNA. The concentration of extracted RNA was determined with a ND-1000 spectrophotometrer (Nanodrop) and total RNA quality was analyzed by capillary electrophoresis with the Agilent 2100 bioanalyser (Agilent technologies) with an Agilent RNA 6000 Nano Assay Ki