RT-PCR of CPER recovered SARS-CoV-2 isolate 3, transfected in ACE2-HEK293 and passaged in Vero E6 cells
PCR products were amplified using the ARCTIC v2 primer set and purified using AMPureXP beads (Beckman Coulter, USA) and subjected to End Repair/dA-Tailing using the NEBNext Ultra II Module (New England Biolabs, USA) and multiplexed using the Native Barcoding Expansion kit (EXP-NBD104, Oxford Nanopore, UK) and Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore, UK).