GSM6215448: Jurkat cells, DMSO and PHA; Homo sapiens; RNA-Seq
After treating with DMSO and JQ1 in the absence or presence of PHA for 24 hours, the Jurkat cells were harvested and RNA was extracted with TRIzol reagent. Amplified cDNA was successively converted into short read sequencing libraries using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina®. Hisat2 (version:2.0.4). Libraries were sequenced on an Illumina HiSeq2500.