High Molecular Weight DNA Extracted from MSU-160 for ONT Sequencing
Tissue samples for genome sequencing were isolated from a single an adult female (MSU-160) Paramormyrops kingsleyae captured from Apassa Creek, Gabon.  Sex was verified post-mortem by inspection of gonads. The electric organ discharge was verified as triphasic. High molecular weight genomic DNA was isolated from fin clip tissue using a Circulomics NanoBind Tissue Big DNA Kit (NB-900-701-01) following the TissueRuptor Homogenizer Protocol (v0.17). Extracted gDNA was verified for concentration and quality using a Nanodrop and Qubit, and high molecular weight DNA was confirmed on a 0.5% agarose gel.   Sequencing libraries were constructed using a Voltrax V2 device with the Voltrax Sequencing Kit (VSK-002).   Based on previous assembly size of 885mb of the Paramormyrops kingsleyae genome we targeted 20x coverage using two Oxford Nanopore R9.4.1 flowcells, we obtained 2,250,969 reads with an effective coverage of 16.89 Gb.