RT-PCR of CPER recovered zsGreen-SARS-CoV-2 isolate 2, transfected in HEK293 and passaged in Vero E6-TMPRSS2 cells
PCR products were amplified using the ARCTIC v2 primer set and spiked in zsGreen CPER primers and purified using AMPureXP beads (Beckman Coulter, USA) and subjected to End Repair/dA-Tailing using the NEBNext Ultra II Module (New England Biolabs, USA) and multiplexed using the Native Barcoding Expansion kit (EXP-NBD104, Oxford Nanopore, UK) and Ligation Sequencing Kit (SQK-LSK109, Oxford Nanopore, UK).