GSM5212082: tub_rep1; Drosophila melanogaster; ATAC-seq
Samples were lysed in Lysis Buffer and Lysates were centrifuged at 500g for 5 minutes at 4ºC to isolate the nuclei. Assay for Transposase-Accessible Chromatin with high throughput sequencing (ATAC-Seq) experiments were performed as described in Buenrostro et al., 2015. Curr. Protoc. Mol. Biol. 109, 21.29.1-9. doi:10.1002/0471142727.mb2129s109