RNAseq of mouse skin neutrophils
Total 5day wounded  skin were digested with liberase into single cell suspension.  Live and single neutrophils were sorted based on markers CD31-Itga6-CD140a-CD45+CD11b+MHCII-Ly6G+ directly into TRIZOL.  Total RNA (10-30ng) was isolated and submitted for RNAseq.  Pooled 10 mice.