GSM6215657: C646 + 4hr WO Rep 2; Drosophila melanogaster; RNA-Seq
Total RNA corresponding to 0.5-1 million control or drug treated S2 cells were homogenized in TRIzol LS (Ambion) and purified and concentrated using the RNeasy MinElute Cleanup kit (Qiagen) according to the manufacturer's instructions. RNA extractions were performed on three independent biological replicates. RNA libraries were prepared from total RNA using the Illumina Stranded mRNA Prep kit and sequenced single-end (1 x 75 bp) on the Illumina NextSeq 2000 platform.