Embryonic day 8 female germ cells
DAZL::GFP germ cells were collected at various stages of chicken embryogenesis. Living DAZL+ cells (GFP+/PI-) were sorted through FACSAria III (BD Biosciences). Libraries for scRNA-seq were prepared using the Chromium Single Cell 3 GEM, Library & Gel Bead Kit v3 (10X Genomics), Chromium Single Cell B Chip kit (10X Genomics), and Chromium i7 Multiplex Kit (10X Genomics). We aimed to capture 800~5,000 single-cell transcriptomes. Gel bead-in-emulsions (GEMs) was generated using Chromium Controller (10X Genomics). Reverse transcription was conducted using thermal cycler (C1000 Touch thermal cycler, Bio-Rad).