ddRAD-seq of Foxtail millet (Setaria italica) genotype GS-458 (Si165)
DNA was isolated from leaves of 4 weeks old plants from 192 accessions using CTAB method (Rogers and Bendich 1985). Post isolation, RNase treatment was performed and the quality and quantity of the DNA was verified in 1.2 % agarose gel followed by quantitation using NanoDrop 1000 (Thermo Scientific). The QC of the samples were further performed using Agilent Bioanalyzer before proceeding for genotyping.  Double digest restriction associated DNA (ddRAD) sequencing approach (Peterson et al. 2012) was used for genotyping the samples.