GSM6297257: NanogKO:Esrrb (44J) rep1 S2; Mus musculus; RNA-Seq
Samples were flow sorted using AriaIII (BD Biosciences, BD Diva Software) cell sorter, for PECAM+PDGFRA- cells. Total RNA was collected using the RNeasy Mini Kit (Qiagen). Genomic DNA was eliminated by Dnase treatment (Qiagen). Ribosomal RNA was removed using the NEBNext rRNA depletion kit. Sequencing libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB)