RNA-Seq of Homo sapiens cumulus cells: Infertile women
Starting with total RNA, messenger RNA was first purified using poly-A selection, then chemically fragmented and converted into single-stranded cDNA using random hexamer priming. Next, the second strand was generated to create a double-stranded cDNA that was ready for library construction workflow. Library construction was carried out in accordance with the TruSeq RNA Sample Preparation v2 (Illumina Inc, USA) protocol. The TruSeq Cluster Generation Kit v5 (Illumina Inc, USA) was used for RNA-Seq, as instructed by the manufacturer.