rumen metagenome: adult male cattle
Rumen content was obtained from a healthy ruminally fistulated beef cattle before morning feeding. The rumen contents were divided into liquid phase and solid phase after filtering through four layers of cheesecloth to distinguish the liquid phase microorganisms existing in the liquid and the solid phase microorganisms attached to the feed surface.  The samples including liquid phase and solid phase were sent to a commercial sequencing company for combined metagenomic sequencing using BGI MGISEQ-2000 and Oxford Nanopore PromethION platform.