metatranscriptomic of rhizosphere sample: RNA-Seq of metaorganism
2 grams of rhizosphere medium were taken at the end of the experiment, and the Rneasy PowerSoil (Qiagen) extraction kit was used. Extract quantity and quality were verified with the BioAnalyser 2100. Libraries were prepared using a TruSeq Stranded Total RNA and ribodeplete using the Ribo-Zero Plant Removal Kit. Samples were sequenced using the Novaseq 6000 Illumina platform with paired-end (PE) readings of 150 bp.