GSM5830965: RNA-P1-cKO-5; Mus musculus; RNA-Seq
Flash-frozen cardiac samples were homogenized in 1 ml of TRIzol (Thermo Fisher Scientific, 15596026) in Precellys Evolution (3 cycles x 20 s at 6800 rpm). RNA as isolated using RNeasy Micro Kit (Qiagen, 74004) as per the provider's instructions. RNA-seq libraries (n=3 mice per genotype) were prepared using KAPA mRNA HyperPrep (Kapa Biosystems, kk8580) kit following manufacturer's specifications.