Transcriptome of Wolfiporia cocos
beads coated with oligo (dT) were used to isolate poly(A) mRNA from total RNA. Fragmentation buffer was added to the mRNA to generate short fragments of 200 701 bp. A random hexamer primer was used to synthesise the first-strand cDNA, using the fragmented RNA as templates. The second-strand cDNA was synthesised using buffer, dNTPs, RNase H, and DNA polymerase I. Short fragments were purified using the QiaQuick PCR extraction kit and resolved with EB buffer for end reparation and poly(A) addition (Qiagen Inc., Beijing, China). The short fragments were connected with sequencing adapters, and following agarose gel electrophoresis, suitable fragments were selected as templates for PCR amplification. Finally, the library was sequenced using the Illumina HiSeq GA system (Illumina Inc.).