Sacoproteus smaragdinus transcriptome
Total RNA was extracted from tissue using the Omega Bio-Tek E.Z.N.A. Mollusc RNA Kit. RNA concentration was measured with a Qubit 3 fluorometer, and purity with a Nanodrop spectrophotometer; RNA quality was assessed from Bioanalyzer traces (Agilent). cDNA libraries were prepared using the Takara SMART-Seq HT Plus kit and sequenced by Illumina NovaSeq 6000.