RNAseq of S. lycopersicum StrippedGerman: Flooding3
Samples were taken from 40-day old tomato plants that were subjected to flooding for 5 days and/or herbivory for 2 days. Plants that were subjected to both treatments were exposed to herbivory on the last 2 days of the 5-day flooding period. Control samples consisted on 40-day old tomato plants with no flooding or herbivory. Samples were obtained by cutting the tip of the leaflet closest to the stem from the second true leaf, using sanitized micro-dissecting scissors (re-sanitized for each sampling). Samples were individually collected in sealable plastic sleeves, sleeves were heat sealed, and immediately flash-frozen by submerging in liquid nitrogen