N_L_3
RNA isolation and cDNA library preparationTotal RNA was isolated from collected tissues using RNAprep Pure Plant Kit (Beijing, China) following manufacturers protocol (Zafar et al. 2021). RNA integrity and concentration was determined on agarose gel and Nanodrop, respectively. High quality messenger RNA was treated with Oligo (dT) and subjected to fragmentation to create short fragments. After purification of cDNA, polyA tails were added to both ends, ligated with adapters and used for library preparation. A total of 12 cDNA libraries were sequenced using Illumina HiSeq 2000 to obtain short sequences from both ends.