GSM5140887: CBAM-68552_RNA_VTP_0.1pctfood_21d_REP5_191008_AGTTCC_S14; Homo sapiens; RNA-Seq
For RNA isolation, cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturers instructions for cell ine experiments. RNA samples from in vivo experiments were obtained by first isolating human cells from mouse marrow using the EasySep mouse-human chimera kit (Stem Cell Technologies) followed by RNA extraction using the PicoPure RNA isolation kit (Thermo Fisher). Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA). Sequencing was done using the Illumina Next Gen Sequencing NextSeq platform (Illumina, San Diego, CA) with 37bp, paired-end reads.