GSM5904905: L4 stage animals, u49, OP, 3; Caenorhabditis elegans; RNA-Seq
Animals were washed off the plates with M9 solution and frozen in TRIzol (Transgen, ER501-01-01). Total RNA was obtained using the kit (Transgen, ER501-01) and 1 ug total RNA from each sample was used as input material for the RNA sample preparations. RNA libraries were prepared for sequencing using standard Illumina protocols.