GSM5141062: R7938 (1X-TETD #4); Mycobacterium tuberculosis H37Rv; RNA-Seq
The TETD tretaed M. tuberculosis cultures were processed for total RNA isolation by TRIzol reagent as per manufacturer's protocol. Mtb total RNAs were analyzed using an Agilent Bioanalyser for quality assessment with a RNA Integrity Number (RIN) range of 6.2 to 9.7 and a median of 8.1. Ribosomal RNA (rRNA) were depleted from 500ng of bacterial RNA using RiboMinus™ Bacteria Transcriptome Isolation Kit (Invitrogen Thermo Fisher Scientific), according to manufacturer's protocol. cDNA libraries were prepared from rRNA depleted mRNA using Lexogen SENSE Total RNA-Seq Library Prep Kit according to manufacturer's protocol. The length distribution of the cDNA libraries was monitored using a DNA High Sensitivity Reagent Kit on the Perkin Elmer Labchip. All samples were subjected to an indexed paired-end sequencing run of 2 x 151 cycles on an Illumina HiSeq 4000 system.