GSM5711838: DscamKO-5, RNASeq; Mus musculus; RNA-Seq
Total RNA was extracted from cerebellum tissues of 5 control and 5 KO mice at P21 using RNeasy Plus Universal Kit (Qiagen). Quality and quantity measurements of extracted RNA were performed using NanoDrop (Thermo Fisher Scientific) and Qubit Fluorometer (Thermo Fisher Scientific), respectively. Sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for directional libraries (New England BioLabs) and the KAPA HTP Library Preparation Kits (KAPA Biosystems) according to the manufacturer's instructions. The RNA-seq libraries were each sequenced (151 cycles) using the Illumina NovaSeq platforms.