GSM5444706: NL-1; Mus musculus; RNA-Seq
LSK cells from the BM of LIP-subjected mice or unligated control mice were sorted by flow cytometry. Three mice were pooled for each replicate.  Total RNA was extracted by Trizol (Invitrogen) and DNA was removed by using TURBO DNA-free Kit (Invitrogen). PolyA-selected mRNA libraries were generated following the manufacturer's protocols (BGI). Samples were sequenced on BGISEQ platform to generate 100 bp paired-end reads with an average depth of 45.0 M reads per sample.