Metagenomic analysis of mice gut microbiota: response to the prebiotic based on alpha-1,3-oligosaccharides
NGS and bioinformatic analysis were performed by Genomed S.A. (Warsaw, Poland)The amplification of V3-V4 region of the 16S rRNA gene for the library preparation were done with 341F and 785R primers. Adaptor sequences, low quality NGS reads, and chimeric sequences were removed. OTUs were constructed in QIME (97%). Finally sequences were trimmed. Taxonomy assigment was performed using Silva dtabase v 138.