short-read of phage resistant Vibrio fischeri ES114 #3
Colonies were struck out on LBS plates from frozen glycerol stocks. Overnight cultures were grown in LBS medium at 28C, then genomic DNA was extracted using Qiagen Blood and Tissue Kit. DNA was sent to SEA-PHAGES at University of Pittsburgh, where it was quantified by Quibit, prepped with NEBs Ultra II FS Kit, run on Bioanalyzer, and run on Illumina Miseq qith v3 kit.