GSM4977584: 3_P_putida_WT_1hr_PYR_3_25_20_3; Pseudomonas putida; RNA-Seq
Cells were collected via centrifugation and immediately resuspended in 600 µl of ~60 C RNAzol. Total RNA was then extracted from these samples using Qiagen's RNeasy Miniprep Kit. Residual genomic DNA was removed from each sample through DNaseI treatment (NEB), and the RNA was re-purified using Qiagen's RNeasy kit. Following RNA extraction and purification using the RNeasy miniprep kit, RNA was percipitated overnight and enriched for mRNA using the Microbexpress protocol. Libraries of the mRNA enriched samples were constructed using the BIOO Scientific NEXTFlex mRNA-Seq protocol. Library concentrations were measured via Qubit and Bioanalyzer and pooled libraries were submitted for RNA-Seq analysis.