16S metagenome of the Laptev sea water column, methane seep, depth 25m
32 PCR cycles with primers for V4 region 515F (5 '-GTGBCAGCMGCCGCGGTAA - 3 ) and Pro-mod-805R (5  -GACTACNVGGGTMTCTAATCC - 3 '), 14 PCR cycles with Illumina index primers (TruSeq)