GSM5905813: RPE1 Dam-PolR2F_full (set3.samplenr_021) DamID; Homo sapiens; OTHER
Cells were harvested by trypsinization, and genomic DNA was extracted by column purification. DamID sequencing was performed as previously described (doi 10.1038/s41596-020-0314-8). Briefly, genomic DNA was digested with DpnI, followed by indexed adapter ligations, bead cleanup and amplification by T7 RNA polymerase. RNA sequencing libraries were prepared by reverse transcription, PCR-based amplification of cDNA, and single-ended size selection. Libraries were sequenced on the Illumina HiSeq 2500 or NextSeq 500.