RNAseq of C.elegansRNA
N2 worms were transferred to indicated RNAi plates at L4 stage and collected at day 2 of adulthood. RNA were extracted from about 200 adult worms using QIAGEN Rneasy Mini kit. RNA-seq libraries were prepared by the RNA sequencing service provider. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and fragmented for cDNA synthesis. After cDNA synthesis and adaptor ligation, fragments of preferentially 370~420 bp in length were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.