GSM5444555: Male_2; Drosophila pseudoobscura; miRNA-Seq
We extracted total RNA form samples using TRIzol (Life Technologies) as recommended by the manufacturer. RNA was dissolved in RNase-free wate For small RNA sequencing we used the TruSeq Small RNA Sample Preparation Kit (Illumina) to generate the cDNA library and we selected constructs of sizes 145 to 160 bp in a 6% PAGE gel, and precipitated in ethanol