GSM5994483: Sma8P; Homo sapiens; RNA-Seq
IPF patients were diagnosed based on                     multidisciplinary review and published criteria. Non-IPF donor lungs not                     suitable for transplantation were obtained from the Regional Organ Bank of                     Illinois. IPF lungs were grossly examined within 6-12 hours from the time of                     explant, and multiple 1cm3 biopsies were obtained from visibly non-fibrotic                     central (IPF.C) and fibrotic peripheral (IPF.P) areas. Non-IPF donor lung                     biopsies of central (CTR.C) and peripheral (CTR.P) were similarly obtained.                     H&E-stained biopsies were reviewed and confirmed by two clinical                     pathologists Total RNA was isolated using TRIzol (Invitrogen) following                     manufacturer's protocol. All RNA samples displayed RNA Integrity Number (RIN)                     >7. RNAseq including cDNA library preparation was processed at the Genomics                     Core Facility of University of Chicago (https://fgf.uchicago.edu/). Total RNA in                     the amount of 100-500µg per sample was depleted of ribosomal RNA using the                     Ribo-Zero kit (Epicentre, Madison, WI). The directional (first strand) cDNA                     libraries were prepared following the guide of TruSeq Stranded Total RNA Sample                     Preparation kit.