Sequencing of transposable element enriched library of Saccharomyces hybrids: control hybrid
We started with a sheared genomic library of 800bp fragments, large enough to span the LTR region of Ty elements and capture flanking genomic sequence. We created a biased library by using custom primers that amplify DNA fragments which contain a full length Ty1 or Ty2 element. We then used a custom sequencing primer that sequences off the LTR, capturing the flanking genomic region. These reads can be mapped back to a reference genome, thus identifying locations of new, non-reference, and reference TE insertions.