GSM5966791: Piezo1fl/fl; FAD; biological repetition 01.; Mus musculus; RNA-Seq
RNA from freshly isolated primary microglia in various groups was extracted with TRIzol reagent (Invitrogen) according to the manufacturer's instructions. mRNA was purified using poly-T oligo-attached magnetic beads (Vazyme Biotech Co., Ltd). And RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The library fragments (370 ~ 420 bp in length) were processed with the AMPure XP system (Beckman Coulter, Beverly, USA). After the PCR products were assessed using library quality on the Agilent Bioanalyzer 2100 system, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia). Then, the library preparations were sequenced on an Illumina Novaseq platform, and 150 bp paired-end reads were generated.