miRNA-Seq of Cervus elaphus kansuensis: antler cartilage at rapid growth stage (60d)
The cartilage tissues in the antler tips of Cervus elaphus kansuensis rapid growth stage (60d) were collected from three individuals for RNA extraction. Total RNA was extracted using TRIzol Reagent according to the manufacturers protocol, then used for sequencing on Illumina HiSeq 2500 platform. The sequencing data was analyzed to identified known and novel miRNAs, differentially expressed miRNAs, and function annotation.