GSM5284306: 4_KO_NT; Mus musculus; RNA-Seq
Cells with/without 1uM H2O2 treatement were lysed with RLT lysis buffer containing 1% beta mercaptoethanol after 24 hours and RNA was harvased using QIAcube as per Rneasy kit from Qiagen. RNA libraries were prepared for sequencing using llumina TruSeq Stranded mRNA Library Prep. The mRNA-Seq samples were sequenced on NextSeq using paired-end sequencing.