Whole genome sequencing of laboratory strain Clostridium botulinum Beluga WT
Genomic DNA extracted from 2-ml overnight TPGY liquid cultures using Wizard Genomic DNA Purification Kit (Promega, WI, USA). DNA concentration was measured using NanoDrop ND1000 spectrophotometer (Thermo Fischer Scientific, MA, USA). Genomic DNA was sequenced using the NGS platform HiSeq2000 (Illumina, CA, USA) at the sequencing unit of the Institute for Molecular Medicine (University of Helsinki, Finland),