GSM6215219: Ldlr-/-_WD_2; Mus musculus; RNA-Seq
Mice were euthanized by carbon dioxide (CO2) inhalation, and aortic valves were collected into the cold Dulbecco's Phosphate Buffered Saline (DPBS). For each sample, isolated mouse aortic valves from 10 mice were pooled into RNAlater solution. After stabilization, RNA was extracted from aortic valves using the TRIzol Reagent. Sequencing libraries were constructed using amplified cDNA according to TruSeq Stranded mRNA Sample Preparation Guide (Part #15031047 Rev. E, Illumina) for the HiSeq 4000 system (Illumina) and sequenced using the Illumina HiSeq 4000 system with 101-bp read length and paired-end reads, according to HiSeq 4000 System Guide (Document #15066496, Illumina).