ddRAD-seq of Salvelinus alpinus from Ireland (Muscle tissue)
DNA was extracted from fin clips, gillrakers and muscle tissue using the Wizard SV Genomic DNA Purification System (Promega, UK), following the manufacturers instructions. DNA concentration of samples was estimated using agarose gel electrophoresis and the AccuBlue Broad Range dsDNA Quantitation Kit (Biotum) on a CLARIOSTAR plate reader. Libraries were prepared using a modified version of the ddRAD protocol of Peterson et al (2012), as described in Maroso et al (2018). Libraries were sequenced on the Illumina NextSeq550 platform at the Genomics Core Technology Unit (Queens University, Belfast) using paired-end 151-bp sequencing.