GSM5966561: Mouse chondrocytes stimulated with M-LPS EVs 2; Mus musculus; RNA-Seq
The chondrocytes were lysed with TRIzol Reagent (Invitrogen carlsbad, CA, USA) for RNA purification perfumed according to the manufacturer's instructions (NucleoSpin® RNA, TAKARA, Tokyo, Japan). The high-quality DNA-free RNA assayed by determining 28S/18S ribosomal RNA bands with an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) were used for libraries generation (TruSeq Stranded mRNA Sample Preparation Kit; Illumina, Inc., San Diego, CA, USA). Quality of libraries was examined by Bioanalyzer High sensitivity DNA kit (Agilent)