GSM5141056: sqk1-1_dCas9; Arabidopsis thaliana; OTHER
Genomic DNA was treated with RNAse A, recovered with phenol chloroform isolation and then treated with sodium bisulfite using the EZ DNA methylation kit (Zymo Research). PCR-amplified amplicons were gel-purified and pooled in equimolar ratios. The Illumina Nextera DNA Flex kit (https://doi.org/10.1101/779215) was used to prepare the library. Single-end Illumina sequencing of 300 bp with an Illumina MiSeq.