DNA sequence of human, HAE patient
The collection added A base at 3 overhangs after repairing ending that made sure the fragments could pare T base with special adapter, and built single individual DNA library after LM-PCR and purification. The library was enriched 16-24h(47) by array hybridization(Roche NimbleGenMadisonUSA), followed by elution and post-capture amplification. The products were then subjected to Agilent 2100 Bioanalyzer and BMG to estimate the magnitude of enrichment. The qualified products were pooled and quantified according different library quantities, then the singlestrand of library products were prepared for circularization and made DNB, finally, sequenced with PE100+100 on MGISEQ-2000.