Raw UCE sequences of pow528a_Coculatus
We conducted DNA extraction using the Qiagen DNEasy Blood and Tissue Kit (Qiagen Inc., Valencia, CA, USA) following the protocol of Moreau (2014). After this step, library preparation, sample pooling, UCE enrichment, enrichment verification, final pooling, and Illumina sequencing followed the protocols described in Faircloth et al. (2015). All steps except for Illumina sequencing were conducted in the Field Museum Pritzker Laboratory, after which sample pools were sent to the University of Utah DNA Sequencing Core Facility for Illumina sequencing on an Illumina HiSeq 2500 (150 bp paired-end reads; Illumina Inc., San Diego, CA, USA). The DNA Sequence Core Facility also demultiplexed and converted the raw data into FASTQ format.