GSM5966678: MM6_shRNA-3_rep3; Homo sapiens; RNA-Seq
Total RNA from MonoMac6 cells with or without IGF2BP2 knockdown was isolated using the TRIzol reagent (Thermo fisher Scientific). Library construction of 1 µg RNA per sample was made using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.