GSM6346491: ATAC-seq on MDA-MB-231 cells  transfected with a control siRNA rep1; Homo sapiens; ATAC-seq
Cells harvested and washed in PBS were resuspended in 100 µl of cold Cell Lysis Buffer (Tris-HCl pH 7.5 10 mM, NaCl 10 mM, MgCl2 3 mM and Igepal CA-630 0.1%). Lysed cells were centrifuged at 500 g at 4°C for 10 min and the pellets were then resuspended in 100 µl of the Transposase reaction mix (50 µl of 2X TD Buffer from Illumina, cat. n° 15027866) to which 5 µl Tn5 transposase (Illumina, cat. n° 15027865) and 45 µl of Nuclease-free water were added before an incubation of 30 minutes at 37°C. DNA was then purified using MinElute Qiagen columns (Qiagen cat. n° 28004) according to the manufacturer's instructions libraries were indexed using the Phusion® High-Fidelity DNA Polymerase (NEB cat. n° M0530) Sequencing was performed using NovaSeq 6000 (Illumina) in paired-end (50 nt)