intermittent
The metagenomic sequencing approach entails the creation of libraries, the sequencing of those libraries, and the analysis of the resulting data. To begin, 500 ng genomic DNA of each sample was randomly fragmented to 500 bp for paired-end library creation. The Next Ultra DNA Library Prep Kit was used to build the paired-end library (Illumina Inc., San Diego CA). We determined the concentration and purity of DNA using a Qubit 4.0 fluorometer (Thermo Fisher Scientific, Waltham, MA, USA)