RNA-seq of CSF of fast-progressing ALS patients pool 3
Total RNA was extracted from CSF using the miRNeasy Micro kit (Qiagen). 7 L of extracted RNA per sample were combined into age- and sex-matched pools of four to five samples each and concentrated using a SpeedVac at ambient temperature for 40 min from 50 L to 8 L. Each sample pool was then converted into RNA-Seq libraries using the QIAseq miRNA library kit (Qiagen) following the recommended parameters for serum RNA. These libraries then underwent 75 bp paired end sequencing on an Illumina NextSeq 500 machine at the University of Leeds, United Kingdom.