GSM5830858: Intestine_Control rep_5; Sparus aurata; RNA-Seq
Total RNA from each tissue was extracted from five randomly chosen specimens using the TRIzol reagent (Invitrogen, San Diego, CA, USA) following the manufacturer's instructions RNA-Seq libraries were prepared using the NEBNext ultra II directional RNA library preparation kit with a poly(A) mRNA magnetic isolation module (NEB #E7490) in accordance with the manufacturer's protocol (New England BioLabs Inc., Ipswich, United Kingdom)