EisaPacbioSequel
Individuals of E. isabella were collected from partially inbred commercial stocks (Costa Rica Entomological Supplies, Alajuela, Costa Rica). High-quality, high-molecular-weight genomic DNA was extracted from late stages of pupae, dissecting up 100mg of tissue, mainly thorax and wing imaginal disk, frozen in liquid nitrogen and homogenized in 9.2ml buffer G2 (Qiagen Midi Prep Kit) adding 19 l of RNAseA. The samples were then transferred in a 15ml tube adding 0.2 l of Protease K and incubated at ~50 C for two hours. Samples were transferred to Genomic tip and processed with Qiagen Midi Prep Kit (Qiagen, Valencia, CA) following the manufacturer's instructions. DNA was then precipitated using 2ml 70% EtOH and dissolved in water.