GSM4977489: FC-IBC02_Parental_High_Paclitaxel; Homo sapiens; ChIP-Seq
Plates were double fixed in 1.5mM EGS/1% formadehyde, cells were lysed and nuclear extracts sonicated. An equivalent of 5x10^6 cells were sonicated in each tube and used for each ChIP. Samples were pre-cleared and pSTAT3 (CST 9131) was incubated overnight. Libraries were prepared using ThruPLEX DNA-seq kit (Rubicon Genomics) according to manufacturer's protocol