GSM5211679: Total RNA from ENKTL50; Homo sapiens; RNA-Seq
For fresh-frozen samples, total RNA was isolated using the Qiagen RNeasy Mini Kit (Qiagen). Total RNA was extracted from FFPE samples using the RNeasy FFPE kit (Qiagen). For fresh-frozen samples, directional poly-A RNA sequencing libraries were prepared and sequenced as 42-bp paired-end reads on an Illumina NextSeq 500 instrument (Illumina) to a depth of 33.2-48.0 million read pairs. For FFPE samples, directional RNA sequencing libraries were prepared and sequenced as 150-bp paired-end reads to a depth of ~50 million read pairs per sample.