RNA-seq of recombinant Cedar virus with HeV F and G proteins passaged with m102.4 and nAH1.3 mAbs
RNA was extracted from viral culture supernatant using the Zymo Direct-zol RNA kit. RNA was treated with the turbo DNA-free kit. cDNA was generated with random hexamers and Superscript IV followed by second strand synthesis with Sequenase polymerase V2.0. The results cDNA was cleaned and concentrated using the Zymo Clean and Concentrator kit. Library were constructed with Illumina's Nextera DNA kit.