GSM4975740: mouse WT DRG rep1; Mus musculus; RNA-Seq
Lumbar DRGs were isolated from adult C57Bl6 mice and transferred to a dissociation buffer (Dulbecco's modified Eagle's medium supplemented with 10% heat-inactivated Fetal Calf Serum) (Gibco; cat # A38400-02). To generate a single cell suspension, DRGs were subjected to a 2 step-enzymatic dissociation followed by a mechanical dissociation. RNA-seq libraries were constructed using Chromium Single Cell 3' Library, Gel Beads & Multiplex Kit (10X Genomics).  Sequencing was performed on Illumina NextSeq500. Read 1 starts with a 26-bp UMI and cell barcode, followed by an 8-bp i7 sample index. Read 2 contains a 55-bp transcript read.