GSM5870302: HUVEC_PIK3CA-wt_rep4; Homo sapiens; RNA-Seq
Cells were harvested with Buffer RLT (Qiagen, Hilden, Germany) and total RNA of was isolated using RNeasy Mini Kit (Qiagen) according to manufacturer's instructions. RNA-Seq libraries were prepared from total RNA using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, Vienna, Austria) according to the manufacturer's instructions.