GSM4977210: ChIPmentation HEP in vitro induced definitive endoderm 48h rep1; Homo sapiens; ChIP-Seq
The cells collected were fixed with 1% formaldehyde for 8 minutes at RT. The fixed cells were lysed in the lysis buffer. Chromatin was sheared by sonication by using Picoruptor® (Diagenode) with the condition of 10 cycles. The shared chromatin from one million cells was subjected to following magnetic immunoprecipitation and tagmentation using SX-8G IP-STAR® Compact Automated System (Diagenode).The immunoprecipitated samples were stripped from the magnetic beads and subjected to the end repair and reverse cross-linking. The illumine sequence compatible sequencing libraries were amplified by nine cycles of PCR. The sequencing libraries were clean-up by AMPure XP beads (1:1.8 sample vol. to beads vol.; Beckman Coulter). The library size distribution was determined by Bioanalyzer (Agilent Technologies) library concentration. The libraries' concentration was quantified by GenNext NGS Library Quantification Kit (Toyobo Co., Ltd). The ChIPmeantaion libraries were sequenced using 150 bp paired-end reads on the HiSeq X (Illumina).