PacBio sequencing of Mucor plumbeus; fresh mycelium
Total cellular DNA was sheared using a Covaris E220 sonicator to achieve fragments with average size of 500 bp. Then libraries were prepared using the Kapa Hyper kit. Libraries were size selected for insert fragments around 500 base pairs using Pippin Prep automatic DNA size selection system (Sage Science). Libraries were analyzed and quantified using a LabChip GX automated electrophoresis system (Caliper) and pooled.