GSM6338710: Ascl1 ESC Rep2 RNAseq; Mus musculus; RNA-Seq
Cells were washed with PBS, trypsinized and FACS sorted where indicated. Cells then were processed using the Qiagen RNeasy mini kit. RNA was additionally treated with TURBO DNA-free™ kit (Invitrogen) according to the manufacturer's protocols. Integrity of RNA was measured using Fragment Analyzer™. RNAseq-Libraries were prepared using QuantSeq 3` mRNA-Seq Library Prep Kit (Lexogen GmbH).