GSM5379590: FI3; Mus musculus; RNA-Seq
FACS-isolated MuSCs or MuSCs grown ex vivo for 24h in the presence of either DMSO or TubA were snap-frozen. Total RNA was extracted using the RNeasy Micro Plus Kit (Qiagen) according to manufacturer's instructions. Reverse transcription was performed with 5 ng RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara). The cDNA was then sheared with a Covaris S2 ultrasonicator. Library constructions were performed using the Ovation Ultralow Multiplex system (NuGEN). Libraries underwent paired-end 150-bp sequencing at Novogene with an Illumina HiSeq 2000 to a depth of 20-40 million reads.