GSM5444416: REH_HOB_pb_r1; Homo sapiens; RNA-Seq
RNA from the REH cells was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). RNA-Seq libraries were built using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Wilmington MA, USA) with 300 ng of total RNA and 11 cycles of PCR according to manufacturer's recommendation. The quality of the libraries was determined by Bioanalyzer High Sensitivity DNA Analysis (Agilent, Santa Clara, CA, USA).