GSM5769493: NCCIT_SVA_minusDoxy_Rep3; Homo sapiens; RNA-Seq
Cells were lysed in Tri-reagent (Zymo research) and total RNA was extracted using Quick-RNA Miniprep kit (Zymo research). RNA libraries were prepared using NEBNext® Poly(A) mRNA Magnetic Isolation Module, NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® according to the manufacturer's instructions (New England Biolabs). 1 μg of total RNA input was used for each library