16Sv4 of homo sapiens: adult colorectal tissue
Total genomic DNA was amplified by PCR using primers that target the bacterial 16S rDNA v4 region (515f: GTGCCAGCMGCCGCGGTAA; 806r: GGACTACHVGGGTWTCTAAT).  Primers contained adapters for MiSeq sequencing and single-index barcodes (reverse PCR primer) so PCR products could be pooled and sequenced directly.