RNAseq of mouse skin monocytes/macrophages/DCs MHCII+
Total 5day wounded  skin were digested with liberase into single cell suspension.  Live and single innate immune cells were sorted based on markers CD31-Itga6-CD140a-CD45+CD11b+Ly6G-MHCII+  directly into TRIZOL.  Total RNA (10-30ng)was isolated and submitted for RNAseq. Pooled 5 female mice.