WGS of Toxoplasma gondii RH: BRD7929 pool 2 was continuously passed in HFFs for 152 days
Parasite DNA was extracted from in vitro cultures using QIAamp DNA blood kits (Qiagen). Integrity of genomic DNA was evalutate on agarose gell and an aliquot of 1-3 ug of gDNA was submitted for whole genome squencing using the Illumina NovaSeq6000 housed at the Washington University Genome Technology Access Center according to established, in house protocols.