GSM6215670: HCT116 cells, GMDS-AS1 shRNA2, biol rep 3; Homo sapiens; RNA-Seq
HCT116 Cells were harvested and RNAiso Plus (9109, TaKaRa) was used to extract total RNA according to the manufacturer's protocol. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 15 cycles of PCR. Sequencing was performed using 12pM hybridization to a 2x150 paired end flow cell. RNA libraries for RNA-seq were prepared using Illumina TruseqTM RNA sample prep Kit following manufacturer's protocols.