GSM5494114: AD3_BCR; Homo sapiens; OTHER
We collected the Homo sapiens of all the participants at 7 a.m., and PBMCs were isolated by density-gradient centrifugation. After the PBMC suspension was resuspended in phosphate-buffered saline, followed by incubation at 4°C with CD45 antibodies for 20 minutes and 7-aminoactinomycin D for 10 minutes. MoFlo XDP (Beckman Coulter) was used for cell sorting. CD45+ cells were selected by gating on the basis of CD45 high, side-scatter low populations for subsequent single-cell RNA sequencing. RNA libraries were prepared with Chromium Single Cell 5' Library & Gel Bead Kit, Chromium Single Cell V(D)J Enrichment Kit, Human T Cell, and Chromium Single Cell V(D)J Enrichment Kit, Human B Cell according to the manufacturer's protocol.