GSM6294410: KOD1-KO-sample-experiment-2; Thermococcus kodakarensis; ncRNA-Seq
Total RNA was isolated from T. kodakarensisand and from tk1545 knock-out strain using TRIZOL reagent (Invitrogen). Small RNA libraries NEBNext® Small RNA Library Prep Set for SOLiD™ Collected RNAs were subject to adaptor ligation, revere transcribed, and PCR amplified. The amplified cDNA library was size selected (100 - 150 nts) by gel purification.