RNA-Seq of Oryza sativa: Mixture of 96 individually barcoded plant samples from the leaf blades and crown root tips of 4 biological replicate plants each of 6 accessions that were paired in wet and dry field environments
Total RNA was processed for each individual sample according to a barcoded plate-based 3 mRNA-seq protocol, as described previously (Groen et al., 2020). This modified version of the SMART-seq2 and SCRB-seq protocols allowed multiplexed pooling of 96 samples before library preparation with the Nextera XT DNA sample prep kit (Illumina). The protocol returned 3-biased cDNA fragments similar to the Drop-Seq protocol (Macosko et al., 2015).