GSM5531040: 1832_D21_DB_pos; Homo sapiens; RNA-Seq
FACS-sorted LMP2 multimer-positive and multimer-negative T cells were harvested. RNA was isolated using Trizol reagent in combination with the PureLink RNA mini and microcolumn system. Oncomine™ TCR Beta-SR Assay for RNA was used for the construction of sequencing libraries. Sequencing was completed on the Ion S5 semiconductor platform using an Ion 540 chip.