GSM6215238: TIL, patient P1, post, TCR; Homo sapiens; RNA-Seq
PBMCs were isolated from whole blood by Ficoll centrifugation. Tumor biopsies were mechanically and enzymatically digested. CD45+ CD3+ T cells were sorted from all samples by flow cytometry then washed with 0.05% BSA in PBS for sequencing. Libraries were constructed according to the standard protocol provided by 10X Genomics for 5' v2 immunoprofiling with feature barcoding.