GSM6339332: RNA-seq of WT-FA rep 2; Mus musculus; RNA-Seq
RNAs from two independent biological replicates of ESCs and EpiSCs were extracted with QIAGEN RNeasy Mini Kit. Harvest around of 1 x 106 cells, as a cell pellet or by direct lysis in the vessel.Add 350μl of Buffer RLT. Add 1 volume of 70% ethanol to the lysate and mix well by pipetting. Transfer the sample to a spincolumn placed in a 2 ml collection tube and centrifuge for 1min at 10000 x g. Discard the flow-through. Add 700 μl Buffer RW1 to the RNeasy spin column. Close the lid and centrifuge for 15 s at ≥8000 x g. Discard the flow-through. Add 500 μl Buffer RPE to the spin column. Centrifuge for 1min at 10000x g and discard the flow-through. Then wash samples with 500 μl Buffer RPE in the spin column and elute the RNA with 30–50 μl RNase-free water.