GSM5140595: run_2_reaction_1; Homo sapiens; RNA-Seq
Cells were multiplexed into pools of cells from different patients with the intent of using in silico genetic demultiplexing tools to recover more single cells and reduce batch effect. No samples from the same person were multiplexed into the same pool. Pools were loaded onto the 10x Genomics Chromium Controller using several lanes per pool, and processed using standard protocols for the single-cell 5'v1 kit with TCR and BCR encrichment, which includes cell lysis, reverse transcription, amplification and library generation. Libraries were constructed using standard protocols from 10x Genomics' single-cell gene expression and immune repertoire solution, chemistry 5'v1.