RNA-Seq of X. topical: embryo CRISPR/Cas9 ift80
One-cell stage embryos were injected with ift80 sgRNA and Cas9 protein. Embryos were lysed at stage 22 and RNA isolated. Genomic DNA was extracted from the used columns. HRMA and ICE analysis were performed to confirm successful indel formation. RNA was further purified and Illumina sequenced.