GSM5966781: Mouse hypothalamus Gipr-EYFP, sample 2 [S2_L001]; Mus musculus; RNA-Seq
Fluorescence-activated cell sorting was performed using a BD Influx Cell Sorter to isolate GiprEYFP+ cells. Cells were gated according to cell size (FSC), cell granularity (SSC), FSC pulse-width for singlets, fluorescence at 488 nm/532 nm for EYFP and 647/670 nm for nuclear stain with DraQ5. Single cell cDNA libraries from purified GiprEYFP+ cells were generated using the 10× Genomics Chromium Instrument and single-cell 3' Reagent kit (V3); R1 = 28 bp, R2 = 91 bp