DNBSEQ-G400 paired end sequencing; Single-cell sequencing of HEK293FT cells with molecular spikes and recorded cell size
Single-cell sequencing of HEK293FT cells with molecular spikes and recorded cell size
Individual HEK293FT were dispensed to 384-well plates while recording cell size using an F.SIGHT2.0 (Cytena) while excluding dead cells (NucGreen 488, Thermo Fisher). Each well contained 0.0321 pg of molecular spikes. no extraction was performed Smart-seq3xpress library preparation was done according to the published protocol (Hagemann-Jensen, Ziegenhain, Sandberg 2021, biorXiv https://www.biorxiv.org/content/10.1101/2021.07.10.451889v1)