Single-cell analysis of somatic mutation burden in mammary cells of pathogenic BRCA1/2 mutation carriers: Sample M21-Bulk
"Bulk genomic DNA for respective single cell collections was isolated from small 100-500 ug pieces of mammary gland tissues using DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's protocol. The libraries were sequenced with 2 x 150 bp paired-end reads on Illumina HiSeq X Ten sequencing platforms by Novogene, Inc."