title: scRNAseq of human cornea
design: Single cells suspended in PBS with 0.04% BSA were loaded on a Chromium Single Cell Instrument (10X Genomics). RNAseq libraries were prepared using Chromium Single Cell 3' Library, Gel Beads & Multiplex Kit (10X Genomics). To minimize the presence of doublets in our population, approximately 6000 cells were loaded per lane and 2000-3000 cells per lane were recovered. Single-end sequencing was performed on Illumina NextSeq505