title: Illumina HiSeq 4000 paired end sequencing; RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation
design: RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation
library_construction_protocol: The MIN6-SAM cell line was generated by successive transduction of MIN6 mouse  cells with lentivirus dCAS-VP64_Blast (#61425, Addgene) followed by blasticidin selection (1 µg/mL) and lentivirus MS2-P65-HSF1_Hygro (#61426, Addgene) followed by hygromycin selection (100 µg/mL). MIN6-SAM cells were subsequently transduced with the sgRNA expressing vector lenti sgRNA-(MS2)-zeo (#61427, Addgene). RNA from technical triplicates of 2 independent Hnf1a activating sgRNAs and 2 independent control sgRNAs was extracted. RNA was prepared using RNeasy mini kit (Qiagen) including DNAse I (Qiagen) treatment. RNA-seq libraries were prepared using the Truseq Stranded mRNA Sample Prep Kit (Illumina) following manufacturer's instructions.
attributes.tag: Experimental Factor: genotype
attributes.value: CrISPR/Cas9 knockout of Hnf1a with g2 RNA