title: GSM6339491: CRISPRi_perturb_seq,guide; synthetic construct; OTHER
library_construction_protocol: After 24 hours, infected Calu-3 cells were washed with 1X PBS, dissociated with 10X TrypLE Select Enzyme (Thermofisher Scientific), washed, and resuspended in 1X PBS with 0.04% BSA. Library prep was performed following manufacturer's instructions (10x Genomics Chromium NextGEM Single Cell V(D)J Reagents Kit v1.1); modifications to the manufacturer's single-cell sequencing protocol were made for direct guide capturing and library preparation as previously described (Replogle et al., 2020)