title: GSM5532009: S26; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from plasma samples using the Qiagen miRNeasy Serum/Plasma Kit. Briefly, frozen plasma samples were thawed, vortexed and centrifuged for 16000 x g in 10 minutes and 50 µl was used for RNA extraction following the manufacturer's protocol. Purified RNA was eluted in 50µl H2O and saved at -80C. 4 µl of purified RNA was used in a Small-seq protocol to construct the libraries (Hagemann-Jensen et al., 2018). Small RNA libraries were pooled and sequenced using an Illumina HiSeq 3000 platform for 100 bp single read.
attributes.tag: GEO Accession
attributes.value: GSM5532009