title: GSM5929354: YT-1111-07; Mus musculus; RNA-Seq
library_construction_protocol: For scRNAseq datasets (in vivo), single cell suspension of microglia is obtained by a modified version of mechanical dissociation protocol as described in Bennett et al. Briefly, mice were perfused and brains were harvested and dounced. Microglia were enriched by Percoll density gradient centrifugation. For RNAseq datasets (in vitro), microglia were extracted from neonatal mouse by magnetic activated cell sorting (MACS) with Cd11b-beads and cultured RNA libraries were prepared for sequencing using standard Illumina protocols