title: GSM5252322: KO1_D3_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction and purification were performed using HiPure Total RNA Mini Kit (Magen, R4111-03). RNA concentration was measured using NanoDrop (Thermo Fisher Scientific). RNA was reverse-transcribed into cDNA using the All-in-One cDNA Synthesis SuperMix (Bimake, B24408). Real-time qPCR was performed with the 2x SYBR Green qPCR Master Mix (Biomake, B21203) on the CFX384 Touch Real-Time PCR Detection System. All the mRNA reaction data were analyzed by 2^-dd(Ct) method. All experiments were repeated at least in triplicate. Illumina Hiseq X ten
attributes.tag: GEO Accession
attributes.value: GSM5252322