title: GSM5532506: Sample 46- 104669 GFP- cells, Single Ventral Organoid Day 90; Homo sapiens; RNA-Seq
library_construction_protocol: Organoid Fusions were manually dissected, single organoids were kept whole. Tissue was then dissociated using a gentleMACS dissociator (Miltenyi). GFP+ and GFP- cells were then obatiend via FACS. Libraries were generated with the SmartSeq-2 protocol 2 (Illumina ). Libraries were pooled and sequenced on a HiSeq V4 flow cell (Illumina) with up to 4 billion single-end reads. We sequenced all samples twice to improve the yield and then combined the samples using samtools (htslib.org). Raw files for both sequencing runs are included in this upload.
attributes.tag: GEO Accession
attributes.value: GSM5532506