title: miRNA-Seq of WT seedling
design: The constructed library used Agilent 2100 Bioanalyzer (Palo Alto, CA, USA) and ABI StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) for quality and yield detection, and sequencing using the Illumina NovaSeq6000 by Gene Denovo Biotechnology Co. (Guangzhou, China). And the single-end sequencing was used in our experiment. Each sample produces 10M reads.