title: GSM5929583: dorsal_EC_21hpf_14; Danio rerio; RNA-Seq
library_construction_protocol: Single Red Eos+ cell was manually picked with micromanipulator system (NT-88-V3; Nikon) under fluorescence microscope, then washed with 2% BSA/PBS, and finally transferred into 4.4ul lysis buffer. The smart-seq2 protocol was used for whole transcriptome amplification. Smart-seq2 oligodT captured cDNA libraries were prepared for sequencing using standard Illumina protocols 150bp paired end RNA-seq