title: Amplicon seq of Anopheles gambiae: Mut_progeny_A_dsx
design: Individual PCR reactions were performed per amplicon under non-saturating conditions, before purification using AMPure XP beads and validated using the fragment Analyzer (High Sensitivity Small Fragment Analysis Kit). The amplicons were then normalised individually at 0.54nM concentration and pooled into equal volumes. A second PCR step attached the dual indices and Illumina sequencing adapters using the Nextera XT Index kit, with a final AMPure XP beads purification step, with validation and quantification of the final libraries. Each library was sequenced using an Illumina MiSeq 2 with 2 x 250 bp v2 paired end run