title: GSM4801343: untreated sample_2; Candida albicans; RNA-Seq
library_construction_protocol: The total RNA were extracted according to TRIzol™ reagent protocol All samples were sent to Novogene Corporation Inc. (Sacramento, CA. US) for library preparation and RNA sequencing. Breifly, mRNA were enriched using oligo(dT) beads; Then, fragmented randomly by adding fragmentation buffer, then the cDNA were synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library were completed through size selection and PCR enrichment.
attributes.tag: GEO Accession
attributes.value: GSM4801343