title: RNA-seq of human post-mortem lungs: Group: COVID-19 Patient 9, Tech. Rep. 2
design: RNA was extracted using RNeasy Mini Kit (Qiagen) according to the manufacturers protocol followed by a DNAse treatment with Turbo DNAse Kit (Thermo Fisher Scientific) at 37C for 30 min. The concentration was determined using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) and the quality was analyzed using the Agilent 2200 TapeStation System with the RNA ScreenTape assay. For RNA-sequencing (RNA-Seq), libraries were prepared using Illumina RNA Prep with Enrichment kit, nt, IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation (Illumina) and Illumina Exome Panel  Enrichment Oligos Only (Illumina).  Libraries were validated by quantification using Qubit dsDNA HS Assay kit (Invitrogen) and size measurement using Agilent 2200 TapeStation System with the High Sensitivity DNA ScreenTape assay. Sequencing was performed on Illumina NovaSeq 6000 system to generate 150-bp paired-end reads.