title: H1395 cell line RNA sequencing
design: For RNA sequencing libraries, methods have been developed based on cDNA synthesis using random primers, oligo-dT primers, or by attaching adapters to RNA fragments followed by some form of amplification. RNA can be primed by random oligomers or by an anchored oligo-dT to generate first strand cDNA. If random priming is used, the rRNA must first be removed or reduced. rRNA can be removed using oligonucleotide probe-based reagents, such as Ribo-Zero (Epicenter, Madison, WI) and RiboMinus (Life Technologies, Carlsbad, CA). Alternatively, poly-adenylated RNA can be positively selected using oligo-dT beads.