title: GSM5929280: Rat2 CRL-1764; Rattus norvegicus; Hi-C
library_construction_protocol: Hi-C was performed as described previously (PMID 28435001). Briefly, ~1,000,000 cells were cross-linked with 1% formaldehyde, permeabilized and digested with DpnII. Next, sticky ends were filled with nucleotides including biotinylated dATP, followed by blunt-end ligation, cross-link reversal and DNA purification. This was followed by biotin removal from unligated ends, sonication, and pulldown of biotinylated fragments with streptavidin beads. Finally, the library was amplified, size selected and sequenced using 75bp paired-end sequencing on a NextSeq500.