title: GSM4800524: Total mRNAs ZT69 control rep 2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: To extract mRNAs associated with ribosomes, Translating Ribosome Affinity Purification (TRAP) was performed as previously described (Reynoso et al., 2015). Total and TRAP mRNAs were isolated using biotinylated oligo(dT) and streptavidin magnetic beads (New England Biolabs) as previously described (Townsley et al., 2015) Libraries were prepared as previously described (Townsley et al., 2015) with the modifications previously detailed (Blair et al., 2019). In the final enrichment step, indexed adapter enrichment primers were used (Townsley et al., 2015) and 12 cycles were performed to amplify the libraries.
attributes.tag: GEO Accession
attributes.value: GSM4800524