title: Illumina NovaSeq 6000 paired end sequencing; ChIP-seq data of HTR1A antagonist WAY-100635 maleate treated GT1-7 cells against untreated control
design: ChIP-seq data of HTR1A antagonist WAY-100635 maleate treated GT1-7 cells against untreated control
library_construction_protocol: 1x10^6 GT1-7 cells with different conditions were harvested by RIPA buffer on ice for 30 min.  Genomic DNA was sonciated into 200 bp around fragments, and incubated with the antibodies of CBX4 (#30559, CST) and H2AK119ub (ABE569, Sigma-Aldrich) at 4 oC overnight. This was followed by incubating for 2 h with Protein A beads (Thermo Fisher Scientific) at 37 °C to pull down the bound DNA fragments. We then isolated the DNA fragments from the pull-down by ethanol precipitation. For high throughput sequencing, we added 3'-dA overhang to the CBX4 or H2AK119ub enriched or input DNA and ligated them to the adapter to build a DNA library.
attributes.tag: Experimental Factor: compound
attributes.value: none
attributes.tag: Experimental Factor: dose
attributes.value: 0
attributes.tag: Experimental Factor: immunoprecipitate
attributes.value: H2AK119ub