title: GSM5252786: PV-ADSCs WT; Mus musculus; RNA-Seq
library_construction_protocol: Adipose tissue was digested by 2 mg/ml collagenase type I (Gibico,17100017) and 1mg/ml dispase II (Sigma, D4693) in Hanks' balanced salt solution (HBSS) at 37°C for 30 to 45 minutes with occasional vortex. The digestion was stopped by DMEM/F12 with 10% FBS. The digestion was subsequently passed through 100-μm filter and then another 70-μm filter, followed by a centrifugation at 300g for 5 minutes. The suspension or so-called SVF was mixed with red blood cell lysis buffer (Biolegend, 420301) for 5 minutes on ice. The cells were then centrifuged and resuspended. RNA libraries were prepared for sequencing using standard Illumina protocols
attributes.tag: GEO Accession
attributes.value: GSM5252786