title: Illumina NovaSeq 6000 sequencing; Melanoma-derived extracellular vesicles mediate lymphatic remodeling and impair tumor immunity in draining lymph nodes
design: Melanoma-derived extracellular vesicles mediate lymphatic remodeling and impair tumor immunity in draining lymph nodes
library_construction_protocol: B16F10-luc2 melanoma cells were cultured in DMEM with pyruvate and 10% FBS. Extracellular vesicles were isolated from cell culture supernatant by size exclusion chromatography using iZON qEV columns according to the manufacturer's recommendation. For a complete protocol, please refer to Leary N, Walser S, Dieterich L.C., Methods Mol Biol (in press). Whole RNA from cells and EVs was extracted using the Macherey Nagel Nucleospin kit according to the manufacturer's instructions. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).
attributes.tag: Experimental Factor: fraction
attributes.value: cells