title: GSM4800217: SATC_132; Ovis aries; RNA-Seq
library_construction_protocol: The four adipose depots were collected with VAT obtained from the mesenteric fat surrounding the ventral sac of the rumen, SAT from under the skin in the sternal region, PRAT from around the kidney and ECAT from between the myocardium and the serous/visceral layer. Each adipose depot was immediately flash-frozen and stored at -80°C until processing. To isolate total RNA, adipose tissue was homogenized with liquid nitrogen and dissolved in Trizol reagent (Life Technologies, Carlsbad, CA) and RNA isolated as per manufacturer's recommendations. RNA was then purified and DNAse-treated on a column using the RNAeasy kit (Qiagen, Germantown, MD) according to manufacturer's instructions. RNA purity and integrity were measured using an Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA). RNA with RNA integrity values ≥ 7.0 were converted into cDNA by using QuantSeq 3′ mRNA-Seq Library Prep Kit (Lexogen, Greenland, NH) according to manufacturer's instructions and libraries compatible for Illumina sequencing were generated.
attributes.tag: GEO Accession
attributes.value: GSM4800217