title: HiC library for WGS of Homarus amerianus - library 327
design: Hi-C libraries were prepared by Dovetail Genomics LLC using heart tissue: chromatin was fixed in place with formaldehyde in the nucleus. Extracted fixed chromatin was digested with DpnII, 5 overhangs filled with biotinylated nucleotides, and  free blunt ends were ligated. Crosslinks were reversed and DNA purified from protein. Biotin that was not internal to ligated fragments was removed. DNA was sheared to ~350 bp, and illumina libraries were generated using NEB Next Ultra enzymes. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment. The libraries were sequenced on an Illumina NextSeq using PE 150 reads.