title: GSM5743906: Cas13d, +CUG1, gRNA, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with 300 uL TRIzol and purified using the Direct-zol RNA Miniprep Kit. Ribosomal RNA was depleted from 300 ng total RNA using the NEBNext rRNA Depletion Kit (NEB, #E6310L), and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760L) according to the manufacturer protocol. Libraries were multiplexed with Illumina i5 and i7 barcoding primers, pooled at 4 nM, and sequenced in a 2x76 bp paired-end format on an Illumina NextSeq 500. >14 million paired end reads were sequenced per library and recorded in FASTQ format.