title: GSM6339943: a, liver; Mus musculus; RNA-Seq
library_construction_protocol: A certain number of liver/spleen lymphocytes were taken from mouse tagged with Foxp3GFP (C57BL/6), and CD4+ T cells were obtained by positive screening of CD4 (L3T4) Microbeads (Miltenyi Biotec), then CD4+ Foxp3GFP+ Treg cells were obtained by flow sorting after cell surface staining. According to the manufacturer's introduction, single-cell RNA-seq libraries were constructed using Single Cell 5' Library and Gel Bead Kit, Single Cell V(D)J Enrichment Kit.The libraries were finally sequenced using an Illumina Novaseq6000 sequencer with a sequencing depth of at least 100,000 reads per cell with pair-end 150 bp (PE150) reading strategy.