title: GSM5929371: HCEC shFOXO3 rep2; Homo sapiens; RNA-Seq
library_construction_protocol: FOXO3 knowndown HCEC and scrambled control were harvested. Total RNA was obtained using TRIzol Reagent (Thermo Fisher Scientific) and RNA quality was confirmed by the Bioanalyzer 2100 system (Agilent Technologies). Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit (NEB) and sequenced on Illumina HiSeq X Ten. We set 2 biological replicates for each group to eliminate technical variance.