title: GSM5929320: RvΔsigA - Atc [Day3-ATC1_S5]; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: RNA was isolated from exponentially growing cultures equivalent to 10 O.D.600 units. The collected cells were resuspended in TRIzol reagent (Invitrogen), lysed using a bead beater (MP FastPrep system, MP Biomedicals) as per manufacturer's instructions, and the TRIzol-lysed cell mix was extracted with chloroform, followed by precipitation of RNA using isopropanol. The RNA pellet was dissolved in RNase-free water (30 µl with 2 µl of RiboLock (Thermo Scientific). DNA contamination was removed by DNase I (Invitrogen) treatment, and the RNA was further purified using RNeasy mini columns (Qiagen).