title: GSM5929272: Total 293T4A DOX-2 [total_DOX_2]; Homo sapiens; RNA-Seq
library_construction_protocol: 293T4A cells were treated with DMSO or DOX (2mg/ml - 12hr) followed by cycloheximide treatment for 10 minutes. Total RNA and ribosome-protected fragments were isolated following published protocol (Ingolia et al 2012). Deep sequencing libraries were generated from these fragments and sequenced on the HiSeq 2000 platform. Genome annotation was from the Ensembl database release 75 (http:// www.ensembl.org).