title: GSM5252387: patient4; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA seq, total RNA was extracted using Trizol. For m6A seq, total RNA in macrophage was extracted using Trizol and polyadenylated RNA was purified using the Dynabeads mRNA Purification Kit (Invitrogen). Fragment the RNAs into ~100-nucleotide-long fragments by 10X Fragmentation Reagent(Invitrogen) at 94℃ for 45s and stop it using the 10X Stop Solution(Invitrogen). EpiMark N6-methyladenosine enrichment kit (NEB E1610S) was used for m6A immunoprecipitation (m6A-IP) to enrich the m6A marked RNAs followed by RNA extraction by Trizol. For RNA seq and m6A seq, the library construction was performed with SMARTer Stranded Total RNA-Seq Kit (Takara). For single cell RNA seq, the library was performed using Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1(1000128) purchased from 10X Genomics according to the protocols provided by manufactures.
attributes.tag: GEO Accession
attributes.value: GSM5252387