title: GSM6339855: GRCh37ERCC-ensembl75-genes-T_20171128SC_F04P1_Run1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from 11 formalin-fixed (4%), paraffin-embedded tissue sections (4μm) per biopsy. After scraping the tissue from the slides and deparaffinization, tissue was digested in buffer PKD (Qiagen, USA). RNA was extracted using the RNAeasy FFPE kit, according to manufacturer's protocol (Qiagen, USA). For RNA extraction from PBMCs, 3x10^6 cells were thawed and rested for 1h in RPMI medium supplemented with 10%FBS and Antibiotic-Antimycotic (all from Gibco, UK) at 37°C. After resting, cell lysis and RNA isolation were performed using the RNAeasy Mini kit, according to manufacturer's protocol (Qiagen, USA). RNA was immediately stored at -80°C until further use. Complementary DNA library preparation was performed using TruSeq Stranded Total RNA Library Prep Gold (Illumina, USA, Cat#20020598), following manufacturer's recommendations. Prepared libraries were sequenced on a HiSeq 2500 System (Illumina, USA)
attributes.tag: GEO Accession
attributes.value: GSM6339855