title: Ilumina Sequence Data: Effect of fertilizer and municipal sludge waste on soil microbiome of agro-ecosystems
design: DNA was isolated from soil samples using a commercial kit , followed by PCR amplification using 16S primer targeting V4 hypervariable region [515F (5  GTGCCAGCMGCCGCGGTAA  3) and 806R (5  GGACTACVSGGGTATCTAAT  3)] , later on library preparation and sequencing using Illumina Miseq .