title: ITS amplicon seq of bacteria: caly soil
design: Total metagenomic DNA was extracted by using the PowerSoil DNA Isolation Kit, and examined by using 1% agarose gel electrophoresis before the DNA samples were stored at 20C in a freezer. Then an improved dual-index high-throughput sequencing approach with paired-end 250 nt was used to create the sequencing library of ITS gene (ITS1 region).