title: RNA-seq of Acanthurus triostegus: larval retina D6 24D
design: Retinal transcriptome libraries were prepared from total RNA using the NEBNext Ultra RNA library preparation kit for Illumina at Novogenes sequencing facilities in Hong Kong and Singapore. The concentration of each library was checked using a Qubit dsDNA BR Assay kit (ThermoFisher) prior to barcoding and pooling at equimolar ratios. Libraries were sequenced as 150 bp paired-end reads on a HiSeq 2500 using Illuminas SBS chemistry version 4.