title: GSM5929358: Fibroblasts - Mock+Vehicle treated rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were harvested in TriZol and RNA was extracted using the RNeasy Mini Kit (Qiagen) Libraries for RNA-Seq that were prepared with KAPA Stranded mRNA-Seq Kit. The workflow consists of mRNA enrichment and fragmentation, first strand cDNA synthesis using random priming followed by second strand synthesis converting cDNA:RNA hybrid to double-stranded cDNA (dscDNA), and incorporates dUTP into the second cDNA strand.  cDNA generation is followed by end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane.