title: Full RNA Seq of MCF7
design: NEBNext Ultra II Directional RNA Library Prep Kit for Illumina-E7760 and NEBNext Poly (A) mRNA Magnetic Isolation Module-E7490 were used for libraries for complete RNA sequencing of MCF7 cells. Sequencing was performed on the NextSeq 500 Illumina with FlowCell High 1x75. HISAT2 version 2.1