title: protoplasting-free scRNA-seq of arabidopsis: root tips
design: Nuclei were isolated from the root tips. Then nuclei suspension from previous step were loaded onto the 10X Genomics ChIP and  libraries were  made using 10x Chromium Single Cell 3' Solution V2 kit. To obtain full-length cDNA, we extend the elongation time during cDNA amplification to 2 minutes.  Half of cDNA template was used to construct Illumina library according to the manufacturers instructions.