title: ITS region amplicon sequencing of wheat associated soil metagenome
design: For Illumina ITS region amplicon sequencing, the amplicon library construction was done as described previously in Yergeau et al. (2015) based on a dual-indexing strategy that followed the 16S Metagenomic Sequencing Library preparation Illumina guide (Part #15 044 223 Rev. B). The V4 hypervariable region was amplified using the primers ITS1F and 58A2R (Martin & Rygiewicz, 2005). PCR products were purified, pooled and submitted for 2  250 bp Illumina MiSeq sequencing at the CESGQ.