title: Whole exome sequencing of  the gross tumors of CT26-POLE-KO from Balb/c mouse with anti-PD-1 treatment
design: Whole-exome sequencing libraries were prepared using the SureSelectXT Library Preparation Kit (Agilent). DNA was sheared using an LE220 Focus-ultrasonicator (Covaris), and the fragments were end-repaired, adenylated, ligated to Illumina sequencing adapters, and amplified by PCR. Exome capture was performed using the SureSelect XT Mouse All Exon Kit 49.6 Mb capture probe set (Agilent), and captured exome libraries were enriched by PCR. Final libraries were quantified using the KAPA Library Quantification Kit (KAPA Biosystems), Qubit Fluorometer (Life Technologies), and 2100 BioAnalyzer (Agilent) and were sequenced on a Novaseq6000 sequencer (Illumina) using 2X150 bp cycles.