title: GSM6294268: Patient 3 PRE-treatment FNA PDAC tissue biopsy 2; Homo sapiens; RNA-Seq
library_construction_protocol: PDAC tissue was then isolated, minced with scissors, and digested in ~15-25mL Dulbecco's Modified Eagle Medium (DMEM) with 2mg/mL Collagenase A and 1x DNase I for 25 minutes at 37°C with constant stirring. Digestion buffer was quenched with 3mL fetal bovine serum (FBS) and filtered through 100uM Nylon mesh, pelleted by centrifugation (2000rmp for 5 min) and resuspended in FACS buffer (1% BSA in PBS). Following tissue digestion, processed cells from each sample were encapsulated into droplets and libraries using BD Rhapsody Single-Cell Capture and cDNA Synthesis Analysis System (BD Biosciences) according to the manufacturer's protocol.