title: GSM6261175: Thymus_CD3neg_donor1_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Pediatric thymus from children undergoing cardiac surgery were obtained and used according to and with the approval of the Medical Ethical Commission of Ghent University Hospital (Belgium). Thymus tissue was cut into small pieces and digested with 1.6mg/ml collagenase (Gibco, 17104-019) in IMDM medium for 30min at 37°C to generate a single cell suspension. The reaction was quenched with 10% FBS and the thymocyte suspension was passed through a 70μm filter to remove undigested tissue. Cells were frozen in FBS containing 10% DMSO and stored in liquid nitrogen until needed. Upon thawing thymocytes were FACS sorted for CD3 expression. library construction according to protocol for 10X Genomics Chromium Single Cell 3' Kit v2