title: Plant microbiota
design: Seeds (50 seeds from each genera) and seedlings (one of each treatment) were washed with hypochlorite 2% (v/v) to remove epiphytes microorganisms as we mentioned above. We used plant DNEASY Kit (Qiagen, Hilden, Germany) and for the seeds, DNA Isolation MO BIO (Carlsbad, California, EEUU). Quant-IT PicoGreen reagent (Thermo Fisher) was used to measure DNA concentration of each sample. DNA samples were used to amplified V5-V6 region of 16S rRNA ITS. Also, blocking primers were used in order not to amplified DNA from mitochondria and chloroplast (Arenz et al. 2015). PCR products had a length of 421 pb approximately. PCR products with the extension tails (PE1-CS1, PE2-BC-CS2) allowed the addition of 10 nucleotides barcodes and a specific Illumina sequences in the second low-cycle number PCR, using a Fluidigm protocol: