title: human cell line: HULEC-5A
design: Fragmented RNAs  were subjected to first strand and second strand cDNA synthesis, followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext UltraTM RNA Library Prep Kit for Illumina. The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit2.0 . The libraries were paired-end sequenced.