title: GSM5380589: ACE2PB_5DGE; Homo sapiens; RNA-Seq
library_construction_protocol: B-cells isolated from 7 COVID-19 patient's peripheral blood were seperated into ACE2 reactive and non-reactive population using ACE2 peptides. Each of these patient's B-cells were labelled with Hashtag oligo linked to antibody, which allowed to barcode each patient with unique DNA oligo sequence. Then ACE2 reactive B cells from 7 patients were pooled together and loaded on 10x Genomics Chip G using 5'DGE kit to interogate single cell 5DGE (Digital gene expression) and VDJ sequence in B-cells. Similarly, ACE2 non-reactive cells were pooled together. 10x Genomics
attributes.tag: GEO Accession
attributes.value: GSM5380589