title: GSM5213114: BMDM_6h_LPS_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were directly lysed in 300mL Trizol (Invitrogen) and total RNA isolated with Direct-zol RNA Miniprep kit (Zymo). mRNA was isolated from total RNA and converted into sequencing libraries using mRNA HyperPrep kit (KAPA/Roche) and custom Illumina-compatible unique dual index (UDI) adaptors (IDT) according to manufacture's instructions. Libraries were PCR amplified, quantified with Illumina Library qPCR quantification kit (KAPA/Roche), and pooled for sequencing on HiSeq4000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM5213114