title: GSM4799577: RNA-seq from UC Davis FAANG chicken skeletal muscle rep B; Gallus gallus; RNA-Seq
library_construction_protocol: Total RNA was isolated using Trizol (Invitrogen, Carlsbad, CA) according to the manufacturer's protocol. DNase I (Ambion, Austin, TX) digestion was carried out after RNA isolation and the RNA concentration and purity were determined by measuring absorbance at 260 nm and A260/A280 ratio using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). RNA samples were stored at − 80 °C until further use. Total RNA (1 μg) was subjected to two rounds of hybridization to oligo (dT) beads (Invitrogen, Carlsbad, CA) to enrich poly-adenylated transcripts. Stranded RNA-seq libraries were prepared using the TruSeq RNA Illumina protocol, and libraries were sequenced on an Illumina HiSeq-3000 using 100 bp PE
attributes.tag: GEO Accession
attributes.value: GSM4799577