title: Whole-genome sequencing of Staphylococcus aureus isolates from Cystic Fibrosis lungs
design: DNA was extracted from overnight cultures of S. aureus isolates grown overnight in LB broth using the DNeasy blood and tissue kit. The Nextera Flex library prep kit was used to generate paired-end libraries which were sequenced using the Illumina MiSeq v3 kit.