title: NGS of SARS-CoV-2 Virus Stocks
design: Trizol-extracted RNA converted to double-stranded cDNA, ligated, and isothermally amplified using a QIAseq FX Single Cell RNA Library Kit. Fragmentation and dual-index library preparation was conducted using an Illumina DNA Prep Tagmentation Library Preparation Kit. Denatured libraries were sequenced on an Illumina MiniSeq using a 300-cycle Mid-Output Reagent Kit per the manufacturer's protocol.