title: EdU-seq of UV-treated hela-s3 in late S phase, replicate 1
design: HeLa cells were irradiated with UV at 1.5 or 3.5 hours after                 releasing to the S phase, then incubated with a medium containing EdU for 2 hours                 before harvesting. Genomic DNA were fragmented by sonication and biotin-labeled by                 click reaction, then end-repaired and ligated to the adaptor with NEBNext Ultra II                 DNA library preparation kit according to the manufacturer's instructions. One                 percent of each sample was reserved for input sequencing. Then, rest samples were                 purified by streptavidin beads and PCR amplified. Resulting libraries were sequenced                 by the Illumina NovaSeq system.