title: Illumina HiSeq 2500 paired end sequencing; RNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control
design: RNA-seq of Delta47 human myeloma cells expressing either the FNDC3A protein or a Mock control
library_construction_protocol: Delta47 cell lines were grown in RPMI 1640 medium containing 100 U/ml penicillin and 100 microg/ml streptomycin supplemented with 20% FBS and 1% glutamine.  Total RNA from control delta47 cells and delta47 cells expressing FNDC3A were isolated using the RNeasy kit (Qiagen) Random primed cDNA libraries were constructed from 100 ng of total RNA.
attributes.tag: Experimental Factor: genotype
attributes.value: FNDC3A-HA expression