title: Targeted sequencing of Homo sapiens: OCT4 CRISPR-Cas9-targeted
design: We designed fifteen PCR primer pairs with the Primer3 webtool (http://bioinfo.ut.ee/primer3/) across the POU5F1 locus (chr6:31,157,800-31,178,600), as well as a control primer pair in exon 4 of the gene ARGFX (chr3:121,583,621-121,586,438). These fragments were PCR amplified and then sequenced by Illumina Miseq v3.