title: RNA-seq of Setaria italica:leaves 0% treatment severe stress replicate 1
design: Total RNA was extracted leaves using TRIZOL reagent (Invitrogen) and genomic DNA was removed using the Turbo DNA-free kit (Thermo Fisher Scientific). RIN was  6. RNA from two biological replicates were pooled for sequencing. All processing procedures of RNA-Seq were performed by Beijing Genomics Institute (BGI, China).