title: Microviruses identified in yellow-bellied marmot
design: 5 g of each fecal sample was homogenized by vortexing in 10 ml SM buffer (0.1 M NaCl, 50 mM Tris/HCl-pH 7.4, and 10 mM MgSO4). The homogenate was centrifuged at 10,000 g for 10 min and filtered sequentially through 0.45 m and 0.2 m syringe filters (Sartorius Stedim Biotech, Germany). The viral particles were precipitated using 15% polyethylene glycol (PEG). Following this, the solution was centrifuged at 10,000 g for 10 min. The pellet was resuspended in 500 l of SM buffer and viral DNA was extracted from 200 l of resuspension using High Pure viral nucleic acid kit (Roche Diagnostics, USA) according to manufacturers instructions and circular DNA was amplified using rolling circle amplification (RCA) with TempliPhi 2000 kit (GE Healthcare, USA). The RCA products were used to prepare Illumina sequencing libraries which were sequenced on the Illumina 4000 platform at Macrogen Inc. (Korea).