title: Illumina NovaSeq 6000 paired end sequencing; Analysis of transcriptome in Dicentrarchus labrax
design: Stranded PE150 RNA-SeqÂ experiment using polyA RNA selection and seqencing on a Novaseq 6000
library_construction_protocol: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: project
attributes.value: FAANG
attributes.tag: secondary project
attributes.value: AQUA-FAANG
attributes.tag: assay type
attributes.value: RNA-seq of coding RNA
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation, other
attributes.tag: sampling to preparation interval
attributes.value: 3.0
attributes.tag: extraction protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/UNIPD_SOP_NucleoSpin-miRNA_extraction_20210603.pdf
attributes.tag: library preparation location
attributes.value: Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road
attributes.tag: library preparation location longitude
attributes.value: 0.145393
attributes.tag: library preparation location latitude
attributes.value: 52.231532
attributes.tag: library preparation date
attributes.value: 2021
attributes.tag: sequencing location
attributes.value: Novogene UK Company LTD, Kristien Bateman Unit 25 Science Park Milton Road
attributes.tag: sequencing location longitude
attributes.value: 0.145393
attributes.tag: sequencing location latitude
attributes.value: 52.231532
attributes.tag: sequencing date
attributes.value: 2021
attributes.tag: experiment target
attributes.value: polyA RNA
attributes.tag: rna preparation 3 adapter ligation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: rna preparation 5 adapter ligation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: library generation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/NMBU_SOP_NEBNext-Ultra-directional-RNA-Library-Prep-Kit_for-Illumina-manualE7420_20210702.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna purity 260-280 ratio
attributes.value: 2.01
attributes.tag: rna purity 260-230 ratio
attributes.value: 2.2
attributes.tag: rna integrity number
attributes.value: 7.9