title: HiSeq X Ten paired end sequencing; RNA-seq data of HTR1A antagonist WAY-100635 maleate treated GT1-7 cells against untreated control
design: RNA-seq data of HTR1A antagonist WAY-100635 maleate treated GT1-7 cells against untreated control
library_construction_protocol: 1x10^6 GT1-7 cells with different conditions were stored in 1 ml TRIZOL (Thermo Fisher Scientific) and grinded in liquid nitrogen 100 μl chloroform and fully mixed, then centrifuged with highest speed at 4 oC for 10 min. The supernatant was moved into a new tube, and added the isopropanol with same volume, and centrifuged with highest speed at 4 oC for 10 min. The precipitate was washed by 75% cold ethanol, and dissolved by appropriate DEPC water. The concentration and quality of RNA was measured by Nanodrop 2000 (Thermo Fisher Scientific) and Agilent bioanalyzer 2100 (Agilent, Santa Clara, CA, USA). 4 μg of RNA in each group were used for library preparation by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ipswich, MA, USA) following manufacturer's instructions
attributes.tag: Experimental Factor: compound
attributes.value: none