title: GSM4800682: Ank_1231_HUPO_Ib; Mus musculus; RNA-Seq
library_construction_protocol: Circulating classsic and non-classic monocytes,  and the 5 subsets of ankle synovial tissue macrophages were freshly harvested. All cell subsets were defined and sorted  by Flow-cytometric procedures. Total RNA was extracted from each sorted cell population (> 400 cell count) using Arcturus PicoPure RNA Isolation Kit. Total RNA was employed for library construction.  Full-length cDNA synthesis and amplification were carried out with the Clontech SMART-Seq v4 Ultra Low Input RNA Kit.  Illumina sequencing libraries were prepared from the amplified full-length cDNA with the Nextera XT DNA Library Preparation Kit.The sequencing of the libraries employed an Illumina NextSeq 500 NGS System.  Single 75 bp reads were generated with dual indexing and the libraries were sequenced to an average depth of 21.8 million reads
attributes.tag: GEO Accession
attributes.value: GSM4800682