title: Nasal swab
design: The V3-V4 hypervariable regions of the 16S rRNA gene were amplified using the 341-F (5-CCTAYGGGRBGCASCAG-3) and 806-R (5-GGACTACNNGGGTATCTAAT-3) primers. All PCR steps were carried out using the Phusion High-Fidelity PCR Master Mix.