title: Enhancement of PHA production by a mixed microbial culture using VFA obtained from the fermentation of wastewater from yeast industry
design: pyrosequencing (Keskin et al., 2017) utilizing Roche 454 FLX titanium instruments and reagents following manufacturers guidelines (MR DNA, Shallowater, TX, USA). The eubacteria 16S primers 27F (5GAGTTTGATCNTGGCTCAG-3) and 519R (5GTNTTACNGCGGCKGCTG-3) used amplified a fragment of approximately 500 pb.