title: This study utilized metagenomic sequencing to elucidate the interaction of sugarcane culture with the soil microorganisms involved in the biogeochemical carbon (C) and nitrogen (N) cycles by determining the diversity of genes involved in the metabolism of these elements.
design: Soil DNA was extracted using the FastDNA SPIN Kit for Soil. The libraries were prepared using the Illumina Nextera sample preparation kit (Illumina) and sequenced in the Illuminas HiScanSQ System (Illumina) in a Rapid Run Mode to generate 2  100 bp paired-end reads.