title: GSM5533327: Col-0 Air treatment replicate 2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Conducted as described in O'Malley et al. 2016 (doi: 10.1016/j.cell.2016.04.038) and Chang et al. 2013 (10.7554/eLife.00675). Harvested seedlings were crosslinked with 1% formaldehyde under vacuum for 20 minutes then snap-frozen in liquid nitrogen. Nuclei were extracted from frozen, ground seedlings according to Qiao et al. 2016 (10.1126/science.1225974) then sonicated according to Chang et al .2016 using a Diagenode bioruptor to yield an average DNA fragment size of approximately 150-300 bases. Transcription-factor DNA complexes were isolated by immunoprecipitation with polyclonal anti-GFP raised in goat from David Dreschel (Max Planck Institute for Molecular Cell Biology and Genetics, Dresden, Germany) or, for non-transgenic controls, IgG from goat (supplied by Covance). These complexes were then retrieved using Protein G Dynabeads (Life Technologies). Conducted as described in (Zander,M et al., 2020) DOI: 10.1038/Nat Plants 6: 290-302
attributes.tag: GEO Accession
attributes.value: GSM5533327