title: Chicago library for WGS of Homarus americanus - library 803
design: Chicago libraries were prepared by Dovetial Genomics LLC. 500 ng of HMW DNA from heart tissue was reconstituted into chomatin in vitro, fixed with formaldehyde, digested with MboI, 5' overhangs were filled in with with biotinylated nucleotides and free blunt ends ligated. Crosslinks were then reversed and DNA purified. DNA was then prepped with NEBNext Ultra Illumina library prep reagents and biotin-containing fragments were isolated with streptavidin beads.