title: NextSeq 550 paired end sequencing; Biosynthetic Gene Cluster regulatory sequence library profiled in S. albidoflavus
design: Biosynthetic Gene Cluster regulatory sequence library profiled in S. albidoflavus
library_construction_protocol: Samples were harvested during mid-log growth phase Nucleic acid samples were prepped using beadbeating followed by phenol-chloroform extraction and ethanol precipitation DNA samples were prepped through a two step PCR method to enrich for amplicons of interest. RNA samples were prepped by gene-specific RT and adapter ligation. Subsequently, two step qPCR was performed to add select for sequences of interest and append Illumina adapter sequences
attributes.tag: Experimental Factor: genetic modification
attributes.value: frr