title: GSM5213039: DLD-1-NC2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy mini kit (Qiagen, Germany). Paired-end libraries were synthesized by using the TruSeq™ RNA Sample Preparation Kit   (Illumina, USA) following TruSeq™ RNA Sample Preparation Guide. Briefly,   The poly-A containing mRNA molecules were purified using poly-T oligo-attached   magnetic beads.     Following purification, the mRNA is fragmented into small pieces   using divalent cations under 94℃ for 8 min. The cleaved RNA fragments are   copied into first strand cDNA using reverse transcriptase and random primers. This is   followed by second strand cDNA synthesis using DNA Polymerase I and RNase H.   These cDNA fragments then go through an end repair process, the addition of a single   'A' base, and then ligation of the adapters. The products are then purified and enriched   with PCR to create the final cDNA library. Purified libraries were quantified by Qubit®   2.0 Fluorometer (Life Technologies, USA) and validated by Agilent 2100 bioanalyzer   (Agilent Technologies, USA) to confirm the insert size and calculate the   mole concentration.
attributes.tag: GEO Accession
attributes.value: GSM5213039