title: Illumina HiSeq 1000 paired end sequencing; EVOREPRO: Maize RNA-seq data from pollen grain and pollen tube during the flowering development stage
design: EVOREPRO: Maize RNA-seq data from pollen grain and pollen tube during the flowering development stage
library_construction_protocol: Freshly mature pollen grains were harvested from B73 maize plants cultivated as described in Chen et al. 2017.  Freshly mature pollen grains were harvested from B73 maize plants cultivated as described in Chen et al. 2017.  Total RNA was extracted from three biological replicates of 100 mg pollen grains by using a SpectrumTM Plant Total RNA Kit (Sigma-Aldrich) according to manufacturerâ€™s instructions. 250 ng of total RNA was each used for library construction.  RNA-seq was carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide for the Illumina HiSeq 1000 System (Illumina) and the KAPA Library Quantification Kit (Kapa Biosystems).
attributes.tag: Experimental Factor: organism part
attributes.value: pollen tube