title: UCE of Andrena proxima complex
design: DNA was extracted non-invasively from full body specimens using Nucleospin Tissue Kits. DNA was quantified and normalized to 50ng by Qubit fluorometer before beeing sheared using a Bioruptor ultrasonicator into approximately 400-600bp fragments. Library preparation was performed using KAPA HyperPrep Kit following the manufacturer's protocols at  volume for all steps excluding PCR. Libraries were indexed using Illumina adapters and amplified using 18X PCR cycles. Enrichments were performed on 8 equimolarly pooled libraries at 147 ng/uL concentrations using myBaits UCE Hymenoptera 2.5Kv2P hybridization capture kit following the manufacturers protocol with a hybridization step of 24 hours at 65.  Finally, pooled PCR products were sequenced with an Illumina Miseq using the Illumina v3 kit (2X 300bp).