title: saurogobio punctatus muscle
design: (1) The DNA molecule to be sequenced is broken into 200-500bp long sequence fragments by ultrasonic wave; (2) The broken genome is patched-out; (3) The patched-out fragment is phosphorylated by adding A and 5 'ends to the 3' end; (4) The repaired fragment is connectively linked. (5) PCR amplification was performed on the DNA fragments after connection. (6) Magnetic bead purification was performed on the amplified library.