title: RNA-seq  of HVA1 overexpression doubled haploid transgenic wheat mannitol stress plants: vegetative leaf
design: 1g of the RNA per sample was used to purify polyA-tailed mRNA using polyT oligo-attached magnetic beads. The mRNA was fragmented (100140 bp) and the first and second strand of cDNA synthesis was performed. The cDNA fragments were end-repaired followed by adaptor ligation for PCR purification and enrichment to create the final cDNA library.