title: RNA-Seq of T24 cell
design: cDNA library was prepared with 10 g RNA per sample, and poly-(A)-containing mRNA was purified by using oligo (dT) magnetic beads (Thermo Fisher Scientific, MA, USA) and Oligotex mRNA Kits (Qiagen, Germany) following the manufacturers procedure. The purified products were fragmented into small pieces, which were used as the template to synthesize first-strand cDNA with random hexamer-primers and reverse transcriptase. Then dNTPs, buffer, RNase H, and DNA polymerase were used to synthesize the second-strand cDNA. After end repair process and ligation of adapters, the cDNA fragments were amplified using the PCR Master Mix. The library products were sequenced using an Illumina HiSeqTM 2000. The RNA used in sequencing was the same with the samples for q-PCR analysis.