title: Illumina HiSeq 2500 paired end sequencing; Sequencing of RNAs derived from the purified endoplasmic reticulum of human LP1 myeloma cells expressing either the wt FAM46C protein or the D90G mutant allele
design: Sequencing of RNAs derived from the purified endoplasmic reticulum of human LP1 myeloma cells expressing either the wt FAM46C protein or the D90G mutant allele
library_construction_protocol: Cell lines expressing inducible constructs of wt and mutant FAM46C alleles were induced by administration of 2microg/microl Doxycycline hydrocloride (Sigma cat no. D3447) for 5 days. Total RNA from ER fractions of FAM46C- and D90G-expressing LP1 cells was extracted using 1ml of TRIZOL reagent. Random primed cDNA libraries were constructed from 100 ng of total RNA.
attributes.tag: Experimental Factor: genotype
attributes.value: wild type FAM46C expression