title: PacBio SMRT whole genome sequencing Niphates digitalis
design: This experiment was conducted for de novo assembly of the Niphates digitalis genome. The whole genomic DNA was extracted using the Nonobind Tissue Big DNA kit. One PacBio library were prepared with the SMRTbell Template Prep 1.0 from DNA cut it at 25 kb. The library was size-fractionated with SageELF and the 25 kb fraction was sequenced on the Sequel II system using the Sequel II Binding version 1 and Internal Control Kit 1.0.