title: GSM5811709: MDA-MB-231 Ctrl H3K27ac_CutTag; Homo sapiens; OTHER
library_construction_protocol: Total RNA was extracted by TRIzol reagent (Invitrogen). For RNA-Seq, libraries were prepared for sequencing using standard Illumina protocols. For Cut&Tag, 50,000 cells were harvested and the CUT&Tag assay was performed using a commercial kit (Vazyme, China, TD901-TD902) according to the manufacturer's protocol.