title: Transcriptomic defining unique regions of long-term callus in kiwifruit: Organogenic callus with ball-shaped regions (protuberances) with visible shoot buds (PT-SH) obtained from mature endosperm
design: mRNA was obtained using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). High quantity RNA samples (OD260/280 = 2.0 - 2.2, OD260/230  2.0, RIN  8.0, 28S:18S  1.5) were employed to construct the sequencing library using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (Illumina).