title: Nanopore sequencing of Lime Blue lake sediment
design: Lime Blue sediment were collected and frozen. In the laboratory, sediment was subsampled for DNA extraction with a Qiagen Power Soil kit (Qiagen, USA). Metagenomic DNA sequencing library was prepared using ONT Sequencing Ligation Kit (SQK-LSK110) as described by manufacturer. Libraries were sequenced on a r9.4.1 MinION Flowcell