title: GSM6339450: A0039, 72h, blood; Monodelphis domestica; RNA-Seq
library_construction_protocol: DNA/RNA extraction were conducted using Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo Research, CA) according to the manufacturer's instructions. DNA/RNA quantity was measured using Qubit Fluorometer 3.0 (Thermo Fisher Scientific, MA) with Qubit RNA BR Assay Kit. Samples with a good RNA quality were selected for RNA sequencing libraries. RNA sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA) and NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA). We checked the final concentrations and size distribution of the libraries with Qubit and LabChip GX Touch HT machine (HT DNA NGS 3K Assay) (Perkin Elmer, MA).