title: Sequel sequencing; Pig transcriptome and gene expression atlas
design: Iso-seq cDNA transcriptomic experiment on a PacBio Sequel II (8M, v2)
library_construction_protocol: https://data.faang.org/api/fire_api/samples/ROSLIN_SOP_GENESWITCH_WP1_Iso-seq_20210809.pdf
attributes.tag: project
attributes.value: FAANG
attributes.tag: secondary project
attributes.value: GENE-SWitCH
attributes.tag: assay type
attributes.value: ISO-Seq
attributes.tag: sample storage
attributes.value: frozen, -70 freezer
attributes.tag: sample storage processing
attributes.value: cryopreservation in dry ice (dead tissue)
attributes.tag: sampling to preparation interval
attributes.value: 468.0
attributes.tag: experimental protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: extraction protocol
attributes.value: https://data.faang.org/api/fire_api/assays/ROSLIN_SOP_GENESWITCH_WP1_CHICK_EXTRACTION_DNA_RNA_20201111.pdf
attributes.tag: library preparation location
attributes.value: The University of Edinburgh, Easter Bush Campus, Midlothian EH25 9RG
attributes.tag: library preparation location longitude
attributes.value: 55.866
attributes.tag: library preparation location latitude
attributes.value: 3.2006
attributes.tag: library preparation date
attributes.value: 2021-05-11
attributes.tag: sequencing location
attributes.value: Earlham Institute, Norwich Research Park, Norwich, Norfolk, NR4 7UZ
attributes.tag: sequencing location longitude
attributes.value: 52.6217
attributes.tag: sequencing location latitude
attributes.value: 1.219
attributes.tag: sequencing date
attributes.value: 2021-11-21
attributes.tag: experiment target
attributes.value: rna
attributes.tag: rna preparation 3 adapter ligation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: rna preparation 5 adapter ligation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: library generation protocol
attributes.value: https://data.faang.org/api/fire_api/experiments/ROSLIN_SOP_GENESWITCH_WP1_TeloPrime_cDNA_amplification_20210225.pdf
attributes.tag: read strand
attributes.value: non-stranded
attributes.tag: rna purity 260-280 ratio
attributes.value: not applicable
attributes.tag: rna purity 260-230 ratio
attributes.value: not applicable
attributes.tag: rna integrity number
attributes.value: not applicable