title: GSM5252632: Coch-P2; Mus musculus; RNA-Seq
library_construction_protocol: Cochlear ducts and the utricle were harvested and pooled into Thermolysin (Sigma-Aldrich) for 20 min at 37oC. The Thermolysin was then replaced with Accutase (Sigma-Aldrich) and the tissue incubated for 3 min at 37oC followed by mechanical dissociation, repeating this step 3 times. After inactivation of the Accutase with 5% fetal bovine serum, the cell suspension was filter through a 35µm nylon mesh to remove cell clumps. Library were prepared using the Chromium Single Cell Gene Expression Solution from 10x Genomics
attributes.tag: GEO Accession
attributes.value: GSM5252632