title: GSM5929378: S2-DRSC (0.6U Hia5) - AB1 antibody m6A-IP-seq; Drosophila melanogaster; OTHER
library_construction_protocol: Three million S2 cells were pelleted at 250 x g   for 5 minutes then resuspended in 60 μl of Buffer A (15 mM Tris, pH 8.0; 15 mM   NaCl; 60 mM KCl; 1mM EDTA, pH 8.0; 0.5 mM EGTA, pH 8.0; 0.5 mM Spermidine). 60  μl of cold 2X Lysis buffer (0.1 % IGEPAL CA-630 in Buffer A) was added to each   sample and mixed by gentle flicking then kept on ice for 10 minutes. Samples were   pelleted at 4°C for 5 min at 350 x g and the supernatant was removed.