title: ChIP-Seq of human: adult female peripheral blood mononuclear cells: Sample NS0015_H3K27ac
design: "PBMC samples had nuclei prepared and were crosslinked and frozen. ChIP was then performed in parallel using antibodies to each of three histone modifications, H3K4me3, H3K27me3, and H3K27ac. Libraries were generated using the NEBNext ChIP-Seq Library Prep Reagent Set for Illumina according the manufacturer's protocol. Fifty-cycle single-end sequencings were performed using Illumina HiSeq 2000."
attributes.tag: alignment_software
attributes.value: bowtie 2