title: GSM4800825: 2i-GFPminus-combined-A; Mus musculus; OTHER
library_construction_protocol: DNA was extracted using the DNeasy blood and tissue kit (Qiagen) using the standard procedure described in the handbook For PCR amplification of the library 2 μg of DNA for each sample was used, with the DNA split into four 500 ng reactions. A one-step PCR approach was used with P5 and P7 primers. The P5 and P7 primer were comprised of the P5/P7 flow-cell attachment sequence, the Illumina sequencing primer and the vector binding sequence (primer sequence in shown in Supplementary Table 1.). In addition, to avoid the issue of reduced sequence diversity faced by amplicon libraries, PCR was performed with a mix of P5 primers containing a stagger region of different length.
attributes.tag: GEO Accession
attributes.value: GSM4800825