title: Type2_114_rep1
design: Whole blood was collected in PAXgene Blood RNA tubes. After removal of ribosomal RNA and globin transcripts with the Ribo-Zero Globin Removal kit (Illumina) , stranded libraries were prepared with the TruSeq Library prep kit (Illumina) and hybridized to a flow cell for sequencing with the Illumina HiSeq platform. RNA-seq data was processed using the TrimGalore toolkit1 which employs Cutadapt2 to trim low quality bases and Illumina sequencing adapters from the 3 end of the reads.  Only reads that were 20nt or longer after trimming were kept for further analysis.  Reads were mapped to the GRCh37v75 version of the human genome and transcriptome3 using the STAR RNA-seq alignment tool4. Reads were kept for subsequent analysis if they mapped to a single genomic location.