title: GSM4799936: active_ALKBH5_dox_minus_ip2; Homo sapiens; OTHER
library_construction_protocol: Cells were washed with PBS and lysed with TRIzol (Thermo Fisher Scientific). Then trichloromethane (Guangzhou Chemical Reagent Factory) was used to extract the total RNA from the TRIzol lysate. Isopropanol (Sangon Biotech) was used to precipitates RNA from the mixture. mRNA was separated from the total RNA by using the Dynabeads™ mRNA Purification Kit (Thermo Fisher Scientific) according to the manufacturer's protocol. The m6A immunoprecipitation method was the same as above. Input samples and IP samples were applied for NGS library construction by using the NEBNext® Ultra II Directional RNA Library Prep (NEB) according to the manufacturer's guide. The adaptor ligated DNAs amplified by PCR for 15 cycles were sequenced on the HiSeqX sequencing platform.
attributes.tag: GEO Accession
attributes.value: GSM4799936