title: SO_8668_PM_32
design: Libraries are prepared with Illumina-compatible NEXTflex rapid DNA sequencing Bundle. 300 ng of Qubit quantified DNA was sheared using Covaris S220 sonicator to generate specific fragments with size range of 300-400 bp. The fragment size distribution was verified on Agilent 2200 TapeStation and subsequently purified High prep Beads. Purified fragments were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTflex Rapid DNA sequencing bundle kit protocol. Adapter-ligated DNA was purified using Highprep Beads. Resultant fragments were amplified for 5 cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid DNA sequencing Bundle. Final PCR product was purified with Highprep Beads, followed by QC.