title: GSM4979053: 1uM_MS023_SKaTER_2_days_+DRB_(0_min)_Rep_1; Homo sapiens; OTHER
library_construction_protocol: Poly(A) RNA seq: RNA was extracted using RNeasy® Mini Kit (Qiagen, 74104) following the manufacturer's protocol. SKaTER-seq: RNA was isolated using TRIzol (Thermo, 15596026) at -80 °C. RNA isolation was followed by poly(A)-depletion using the NEBNext Poly(A) mRNA magnetic isolation mod-ule (NEB, E7490L). Poly(A) RNA seq: Stranded RNA seq libraries were constructed by Novogene Genetics US. The barcoded libraries were sequenced by Novogene on an Illumina platform using 150nt paired end libraries generating ~30-40 million reads per replicate. SKaTER-seq: Stranded RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) and KAPA Unique Dual-Indexed Adapters (Roche) according to instructions provided by the manufacturer.
attributes.tag: GEO Accession
attributes.value: GSM4979053