title: GSM4798874: N20_Normal_Normal_N20; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from skin biopsy samples using Qiagen miRNeasy Mini kit. The libraries were generated using TruSeq Stranded mRNA Library Prep kit following manufactory protocol. Briefly, mRNA was first extracted from 240ng of total RNA using oligo-dT magnetic beads and fragmented at high temperature. A cDNA library was then prepared from the fragemented mRNA by revervse transcription, second strand synthesis and ligation of specific adapters. Next generation sequencing was performed on Illumina Hiseq 4000 with single-ended 100 red cycles. Image analysis and bases calling was conducted in real-time by the Illumina analysis pipeline.
attributes.tag: GEO Accession
attributes.value: GSM4798874