title: GSM4799652: W1D4I; Mus musculus; RNA-Seq
library_construction_protocol: after perfusion of phosphate buffer solution, hearts were removed from mice, flash frozen on dry ice, and the apex cordis was seperated for RNA extraction with Trizol reagent. At least 1 µg DNase-treated total RNA, with at least 30% of the RNA fragments with a size >200 nt, was used to generate RNA Access libraries (Illumina) following the manufacturer's recommendations. Library quality was assessed using the 2100 Bioanalyzer (Agilent). The final library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecular, DNBs were loaded into the patterned nanoarray and single end 50 bases reads were generated on BGIseq500 platform (BGI, Shenzhen, China).
attributes.tag: GEO Accession
attributes.value: GSM4799652