title: Illumina NovaSeq 6000 paired end sequencing; Horse transcriptome annotation and gene expression atlas
design: RNA-Seq transcriptomic paired end experiment run on an Illumina NovaSeq 6000
library_construction_protocol: TruSeq Stranded mRNA Sample Preparation
attributes.tag: project
attributes.value: FAANG
attributes.tag: assay type
attributes.value: transcription profiling by high throughput sequencing
attributes.tag: sample storage
attributes.value: frozen, liquid nitrogen
attributes.tag: sample storage processing
attributes.value: fresh
attributes.tag: sampling to preparation interval
attributes.value: 10.0
attributes.tag: experimental protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: extraction protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: library preparation location
attributes.value: 126 Corporate Blvd, South Plainfield, NJ 07080, USA
attributes.tag: library preparation location longitude
attributes.value: -74.42
attributes.tag: library preparation location latitude
attributes.value: 40.55
attributes.tag: library preparation date
attributes.value: 2021-02
attributes.tag: sequencing location
attributes.value: Admera Health, 126 Corporate Blvd, South Plainfield, NJ 07080, USA
attributes.tag: sequencing location longitude
attributes.value: -74.42
attributes.tag: sequencing location latitude
attributes.value: 40.55
attributes.tag: sequencing date
attributes.value: 2021-03
attributes.tag: experiment target
attributes.value: rna
attributes.tag: rna preparation 3 adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: rna preparation 5 adapter ligation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: library generation pcr product isolation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: preparation reverse transcription protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: library generation protocol
attributes.value: https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseqexome/truseq-exome-library-prep-checklist-15059914-01.pdf
attributes.tag: read strand
attributes.value: mate 2 sense
attributes.tag: rna purity 260-280 ratio
attributes.value: 1.994
attributes.tag: rna purity 260-230 ratio
attributes.value: -1.0
attributes.tag: rna integrity number
attributes.value: 9.0