title: GSM6339630: SP, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Cells (CD4+ CD25+ YFP+ and YFP- cells from Mycfl/flFoxp3Cre/WT mice) were sorted by flow cytometry into a well of a chilled 96-well plate (filled with 14 μl PBS with 0.06% BSA). Cells were encapsulated in one lane of a 10x Chromium single cell controller. Single-cell transcriptome library is prepared with a Single Cell 3′ Reagent Kit (V2 chemistry) following standard protocol.