title: GSM6300740: R362N_ctrl_S4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using Trizol and Qiagen RNeasy kit. 500 ng of total RNA was used for library construction. Illumina TruSeq Stranded mRNA Library Prep was used for library construction following the manufacturer's protocol with a modification of 15 to 13 PCR cycles in the "Enrich DNA Fragment" step.