title: GSM4801023: SE4_PMAiono; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy kit (Qiagen) according to the manufacturer's protocol. rRNA removed using the Ribo-Zero kit. Libraries were prepared according to Illumina's instructions. Briefly, the RNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using RNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Then, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment. RNA-Seq (lncRNA library)
attributes.tag: GEO Accession
attributes.value: GSM4801023