title: GSM5145222: WGBS PXGGY; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA). Whole Genome Bisulfite conversions were performed with 200 ng of gDNA using the EZ DNA Methylation-Gold, 50rxn (Fisher-part#50444294) kit. WGBS Libraries were created using the Accel-NGS Methyl-Seq DNA Library Kit - 24 rxns (Swift Biosciences-part#30024) and Accel-NGS Methyl-Seq Dual Indexing Kit - 96rxns (Swift Biosciences-part#38096). The libraries were pooled and sequenced on 0.240 of an NovaSeq S4 flow cell (300 XP; targeting 30x wgbs coverage/120Gb per sample) at the Genome Technology Access Center at Washington University School of Medicine in St. Louis.
attributes.tag: GEO Accession
attributes.value: GSM5145222