title: GSM4798820: H3K4me1_naive_rep1; Homo sapiens; OTHER
library_construction_protocol: WA09/H9 NK2 naive and primed PSCs were kindly provided by Austin Smith (Takashima et al., 2014) with permission from WiCell. All PSCs were cultured in 5% O2, 5% CO2 at 37°C. Naive PSCs were maintained as previously described (Takashima et al., 2014) in a 1:1 mixture of DMEM/F12 and Neurobasal, 0.5x N2 supplement, 0.5x B27 supplement, 1x nonessential amino acids, 2mM L-Glutamine, 1x Penicillin/Streptomycin (all from ThermoFisher Scientific), 0.1mM β-mercaptoethanol (Sigma-Aldrich), 1 μM PD0325901, 1 μM CHIR99021, 20ng/ml human LIF (all from WT-MRC Cambridge Stem Cell Institute) and 2 μM Go6983 (PKCi; Tocris) on Matrigel–coated plates (Corning). Primed PSCs were maintained on Vitronectin-coated plates (0.5 μg/cm2; ThermoFisher Scientific) in TeSR-E8 medium (StemCell Technologies). CUT&RUN samples were generated essentially as described (Skene et al., 2018). Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina. Libraries were paired-end sequenced on an Illumina NextSeq machine at the Babraham Institute Sequencing Facility.
attributes.tag: GEO Accession
attributes.value: GSM4798820