title: DNA-Seq of 2-day-old pupa
design: A two-day-old pupa was fast frozen in liquid nitrogen and preserved at -70C. Genomic DNA was extracted with the Blood and Cell Culture DNA Kit (QIAGEN). Powdered pupal tissue was incubated with RNAse-A and Protease for 2h while gently rocking. DNA was purified by gravity flow with QIAGEN Genomic-tips, precipitated with isopropanol, and washed twice with cold 70% ethanol. One g of unsheared genomic DNA was used.