title: Halls Bayou Summer
design: 5 g of pooled soil samples from Halls Bayou were shipped to Mr DNA (Shallowater TX). DNA was extracted from 250 mg using DNeasy. 16S rRNA gene V4 variable region PCR primers 515/806 for a 30 cycle PCR. Pooled amplicons were used to prepare Illumina DNA librraies fro MiSeq.