title: WGS of Pseudomonas aeruginosa:36-1
design: Clinical isolates of P. aeruginosa were sourced from patients with cystic fibrosis, chronic obstructive pulmonary disease and from other forms of critical infections. For genome sequences generated for this study, the DNeasy UltraClean Microbial Kit (Qiagen) was used to extract gDNA from P. aeruginosa following the manufacturers protocol. Library preparation and sequencing of isolates was carried out using an Illumina MiSeq (Otago Genomics and Bioinformatics Facilities, University of Otago, New Zealand).