title: GSM5719952: Single cell RNA-seq control 1; Homo sapiens; RNA-Seq
library_construction_protocol: The specimens were centrifuged at 400g for 5 min at 4℃, and the supernatant was discarded. Red blood cell lysate (SolarBio, R1010, China) was added and placed on ice for 15 min, followed by centrifugation at 400g at 4℃ for 10min, to precipitate PBMCs. The supernatant was discarded and the PBMCs were resuspended by phosphate buffered saline to obtain a single-cell suspension. Single cell suspension was loaded onto a microfluidic chip and single cell RNA-seq libraries were constructed according to the manufacturer's instructions (Singleron GEXSCOPE Single Cell RNAseq Library Kit, Singleron Biotechnologies)
attributes.tag: GEO Accession
attributes.value: GSM5719952