title: 18S rRNA marine plankton SW Pacific
design: Seawater filtration onto 0.2 um filter - DNA extraction and PCR amplification of rRNA 18S V4 region using  eukaryotic primers V4F_illum (5 CCAGCASCYGCGGTAATTCC-3) and V4R illum (5-ACTTTCGTTCTTGATYRATGA-3)