title: GSM4977919: SNT_TE4.5_rep2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq, we performed reverse transcription directly on the cytoplasmic lysate and used terminal deoxynucleotidyl transferase to add a poly(A) tail to the 3' end of the first-strand cDNAs. We amplified the total cDNA library by 18-20 cycles. Afterward, we fragmented the amplified cDNA by Covaris sonicator (Covaris S220, USA) and used the TruSeq Library Prep Pooling kit (Illumina 15042173, USA) to generate the RNA sequence libraries. Illumina HiSeq 2500
attributes.tag: GEO Accession
attributes.value: GSM4977919