title: RNAseq of Mus musculus male liver MA-CCHFV 3dpi
design: 8 week old  wild-type C57BL/6J mice (stock #000664) were purchased directly from Jackson Laboratories. Mice were randomly assigned to study groups and either mock-infected or infected with 100,000 TCID50 of mouse-adapted CCHFV via the IP route. On day 1 and 3 post-infection mice were euthanized via exsanguination and pieces of livers collected into RNALater (Sigma). Samples were stored in RNALater overnight at 4C, RNALater removed and tissue stored at -80C until RNA extraction. RNA was extracted using TRIzol reagent. Each sample lysate was combined with 1-Bromo-3-chloropropane, centrifuged, and aqueous phase was extracted using Qiagen AllPrep DNA/RNA 96-well system (Valencia, CA) with the additional on-column Dnase 1 treatment.  One microgram of RNA was prepared for sequencing using the Illumina Stranded mRNA Prep Ligation kit.  Libraries were sequenced on a Illumina NextSeq 550 generating paired end 74 bp reads.