title: GSM5252418: infected A549 cells rep1 (miRNA); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent. After removing the rRNAs, half of the total enriched RNA was used for circRNA and mRNA library construction, and the other half was size-selected by polyacrylamide gel electrophoresis to enrich for 18–30 nt RNAs for small RNA library construction. To construct circRNA and mRNA libraries, fragmented RNAs were reverse-transcribed into cDNA by using random primers, and the cDNA fragments were purified with a QiaQuick PCR extraction kit, end repaired, poly(A) added, and ligated to illumina sequencing adapters. Then, Uracil-N-Glycosylase was used to digest the second-strand cDNA. The digested products were amplified by PCR.To construct the small RNA library, the 3' and 5' illumina sequencing adaptors were ligated to the RNAs, and the ligation products were reverse-transcribed, amplified.
attributes.tag: GEO Accession
attributes.value: GSM5252418