title: Microviruses identified in Lake Sarah Potamopyrgus antipodarum and  Potamopyrgus acuta whole tissue
design: Samples were grouped and homogenised in SM buffer [0.1MNaCl, 50mMTris/HCl (pH 7.4), 10mMMgSO4] at a ratio of 10 ml SM buffer per 5 g of tissue/sample, centrifuged (10,000 g for 10min) to pellet tissue and cellular debris. The supernatant was sequentially filtered through a 0.45 m filter and a 0.2 m filter and viral DNA was extracted using the High Pure Viral Nucleic Acid kit (Roche, USA). The circular DNA was enriched using TempliPhi (GE Healthcare, USA). Prior to sequencing, the enriched circular DNA products of P. antipodarum and P. acuta combined to form a Gastropoda group.