title: RNA-seq of Crocodylus palustris : cornea and lens
design: Total RNA was extracted from the pooled cornea and lens tissues, using an AllPrep DNA/RNA/Protein mini kit (Qiagen, Germany) following the manufacturers instructions. The RNA was quantified by measuring the absorbance at 260 nm using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Inc.) and the purity of the RNA sample was assessed at an absorbance ratio of 260/280. RNA Integrity Number (RIN) was measure using BioAnalyzer 2100 (Agilent) and Qubit RNA assay kit (Life Technologies, Carlsbad, CA, USA) was also used to assess concentration, DNA contamination, and overall quality. High quality RNA (RIN >8) was then used for paired-end cDNA library preparation with Illumina TruSeq RNA sample preparation v2 kit (Illumina, San Diego, CA, USA), following the manufacturers protocols. Then 2x100 bp paired-end sequencing was performed on an Illumina HiSeq 2500 (Illumina, US).