title: PacBio transcript analysis of Drosophila simulans Sz12 male head tissue under ethanol treatment.
design: Flies were flash frozen and heads isolated on dry ice. Approximately 100 fly heads underwent total RNA isolation followed by full-length cDNA synthesis using the SMARTer PCR cDNA Synthesis kit. Single molecule SMRTbell libraries were constructed from each double-stranded cDNA sample using the IsoSeq protocol followed by PCR optimization and ELF size selection. Each library was sequenced at UF ICBR (Gainesville, FL).