title: GSM5212954: U2OS_TERT-WT_dsRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol Reagent (Invitrogen). For dsRNA enrichment, total RNA was immunoprecipitated using the J2 dsRNA-specific antibody (English and Scientific Consulting Kft., 10010200). In brief, the product of 9 μg RNA was incubated in binding buffer (150 mM NaCl, 50 mM Tris pH 8.0, 1 mM EDTA, 1% NP-40) with 5 μg J2 antibody and 20 μl Magna ChIP™ Protein A+G Magnetic Beads (Millipore), rotating overnight at 4°C, followed by 5 washes in cold binding buffer. RNA was then extracted with TRIzol Reagent. RNA libraries were prepared for sequencing using strand-specific Illumina protocols Strand-specific RNA-Seq
attributes.tag: GEO Accession
attributes.value: GSM5212954