title: Isolate WGS short reads (forward) of Bacteroides_ovatus : human gut bacteria: Sample 6383DG_0717_019_D12_R
design: "We used the DNeasy UltraClean96 MicrobioalKit (QIAGEN) kit to extract whole genome DNA from isolate colonies, following manufacturers' protocols. Genomic DNA libraries were constructed from 1.2ng of DNA using the Nextera DNA Library Preparation kit (Illumina), following the manufacturer's protocol, with reaction volumes scaled accordingly. Prior to sequencing, we pooled on average 250 samples with equal quantities of DNA. Insert size and concentration of each pooled library were determined using an Agilent Bioanalyzer DNA 1000 kit (Agilent Technologies). Paired- end (2x150bp) reads sequencing was performed using an Illumina NextSeq 500 instrument (Illumina Inc) at the Broad Institute."