title: Microviruses identified in Lake Sarah Chironomus zealandicus whole tissue
design: Samples were grouped and homogenised in SM buffer [0.1MNaCl, 50mMTris/HCl (pH 7.4), 10mMMgSO4] at a ratio of 10 ml SM buffer per 5 g of tissue/sample, centrifuged (10,000 g for 10min) to pellet tissue and cellular debris. The supernatant was sequentially filtered through a 0.45 m filter and a 0.2 m filter and viral DNA was extracted using the High Pure Viral Nucleic Acid kit (Roche, USA). The circular DNA was enriched using TempliPhi (GE Healthcare, USA).