title: GSM4800090: H3K27me3_CINs_P2_Rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Nuclear extracts were prepared from sorted cells and digested with MNase to obtain mono- and di-nucleotide fragments. Digested chromatin was dialysed overnight. Input control chromatin was set aside, and immunoprecipitation reactions were performed with antibodies against H3K27ac (Millipore Cat# 05-1334, RRID:AB_1977244) or H3K27me3 (Millipore Cat# 07-449, RRID:AB_310624). Precipitated fractions were purified using Dynabeads (Invitrogen) and RNase-treated prior to library construction. Libraries were prepared using an Ovation Ultralow DR Multiplex System (Nugen), size selected in the range of 180-300 bp on a BluePippin (Sage Science), quality control tested on a Bioanalyzer (Agilent) and sequenced on a HiSeq 4000 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM4800090