title: Nematostella vectensis associated bacteria transmission
design: the hypervariable regions V1 and V2 of bacterial 16S rRNA genes were amplified. 100 ng of template DNA were added to 25 l PCR reactions, (98 C30 s, 30  [98 C9s, 55 C60s, 72 C90s], 72 C10 min)