title: Amplicon sequencing of piglet tonsil microbiome
design: DNA was isolated from tonsil HydraFlock swabs with using PowerSoil kit. DNA was amplified with 16S rRNA gene V3-V4 region primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3).