title: Amplicon seq of Anopheles gambiae: Wt-Dsx_in_silico_sites_rep2
design: PCRs were performed using Phusion Hot Start Flex DNA polymerase (New England Biolabs). PCR products were then purified using homebrew magnetic beads, quantified using a QuantiFlor dsDNA System kit (Promega), normalized to 10 ng/l per amplicon and pooled. Pooled samples were end-repaired and A-tailed using an End Prep Enzyme Mix and reaction buffer from NEBNext Ultra II DNA Library Prep Kit for Illumina, and ligated to Illumina TruSeq adapters using a ligation master mix and ligation enhancer from the same kit.  Library samples were purified with homebrew magnetic beads, size-selected using PEG/NaCl SPRI solution (KAPA Biosystems), quantified using droplet digital PCR (BioRad) and loaded onto an Illumina MiSeq for deep sequencing