title: Metatranscriptome of ibuprofen enrichment
design: For metatranscriptomic study, total RNA from the pellet (40 ml of enrichment culture centrifuged at 2360 g) of the three months old enrichment cultures was extracted using PureLinkTM RNA Mini Kit (Invitrogen, Thermo Fisher Scientific). Metatranscriptome sequencing was performed as follows: paired-end libraries were prepared using the Zymo-Seq RiboFree Total RNA Library Kit (the protocol includes the universal rRNA depletion step as well). Sequencing was done on an Illumina NextSeq sequencer using the TG NextSeq 500/550 High Output Kit v2 (300 cycles). Primary data analysis (base-calling) was carried out with bcl2fastq software (v2.17.1.14, Illumina).