title: de novo sequencing seq of Vibrio parahaemolyticus: envorionmental strain Vp. 1474 from seafood
design: Total 1g DNA  was used as input material for the DNA sample preparations. Sequencing libraries were generated using DNA Library Prep Kit for Illumina. Briefly, the genomic DNA was fragmented by sonication to sizes of 350 bp and 6 kb. Large DNA fragments were amplified with circularization amplification. The products were randomly disrupted into fragments of about 350bp, and the sequences on both sides of the circularized primers were captured with probes for subsequent DNA library construction. DNA fragments were end-polished, added poly-A tail, and ligated with the full-length adaptor for further PCR amplification. At last, PCR products were purified and the insert size of libraries was analyzed for size distribution, and the libraries were quantified using real-time PCR.