title: GSM5929648: GABA2; Drosophila melanogaster; ATAC-seq
library_construction_protocol: Adult flies expressing neuron-specific GFP were frozen at -80 degrees C for 5 min and the heads were removed by shaking. The heads were homogenized with a Dounce homogenizer, filtered through a 40 micron cell strainer, and washed several times. GFP-labeled nuclei were collected by flow cytometery. Isolated nuclei were treated with Nextera Tn5 enzyme for 23 min at 37 degrees Celsius. The tagmented DNA was purified, amplified by PCR, and purified again using AMPure XP magnetic beads.