title: GSM4798907: negative lymph node-scATAC; Homo sapiens; ATAC-seq
library_construction_protocol: The concentration of single-cell suspension was adjusted to 1×105 cells/mL in PBS.  Cell suspension was transferred to 2-ml tube and centrifuged (300rcf,5 min) to remove supernatant. Then added lysis buffer (Tris-HCl (pH 7.4): 10 mM, NaCl: 10mM, MgCl2: 3mM, Tween-20: 0.1%, Nonidet P40 Substitute: 0.1%, Digitonin: 0.01%, BSA: 1%, right amount nuclease-free waster) to pellet and mixed. Incubated the mixture on ice (3~5min). After that, added wash buffer (Tris-HCl (pH 7.4): 10mM, NaCl: 10mM, MgCl2: 3mM, Tween-20: 0.1%, BSA: 1%, right amount nuclease-free waster) to the mixture, mixed and centrifuged (500 rcf, 5min). The supernatant was removed, and resuspended the pellet by diluted nuclei buffer (10x Genomics, 2000153/2000207). After nuclei concentration was determined by Countess II FL Automated Cell Counter, the resulting nuclei solution was used for library construction immediately. The scATAC libraries were constructed according to the user guide of Chromium Next GEM Single Cell ATAC Reagent Kits v1.1. scATAC-seq
attributes.tag: GEO Accession
attributes.value: GSM4798907