title: GSM5213095: vWAT_mac_E2_rep2; Mus musculus; RNA-Seq
library_construction_protocol: vWAT macrophages were sorted directly into Trizol-LS (Invitrogen) and total RNA isolated according to manufacturer's instructions using chloroform extraction and glycogen-assisted RNA precipitation with GlycoBlue (Ambion). mRNA was isolated from total RNA and converted into sequencing libraries using mRNA HyperPrep kit (KAPA/Roche) and Illumina-compatible NEXTFlex sequencing adaptors (Bioo) according to manufacturer's instructions. Libraries were PCR amplified, quantified with Illumina Library qPCR quantification kit (KAPA/Roche), and pooled for sequencing on HiSeq2500 (Illumina).
attributes.tag: GEO Accession
attributes.value: GSM5213095