title: RNA-Seq of Laminaria digitata: sporophytes at 10 and 20.5C
design: RNA was enriched for polyA+ species and then converted to a cDNA sequencing library using a TruSeq Stranded mRNA kit (Illumina, San Diego, USA) according to the manufacturer's protocol. The cDNA was sequenced as 75 bp paired end libraries on an Illumina HiSeq 4000 at Cologne Center for Genomics, Cologne, Germany. Raw reads were quality trimmed with Trimmomatic (Bolger et al., 2014).