title: 18s RNA-Seq of Mangrove Sediment Eukaryote from Wallacea
design: Total genomic DNA was extracted from 0.7g of surface and depth sediment using PowerSoil DNA Kits (Qiagen Inc). The V9 region of18S gene was amplified using the universal primers Euk1391f and EukBr was carried out using conventional PCR as per the 18S amplification protocol available from the Earth Microbiome Project (http://www.earthmicrobiome.org). All primers used contained an adapter for the sequencing platform and an 8-nucleotide barcode. Libraries were sequenced and pair-end reads were analysed form the operational taxonomic units (OTUs) of 97% sequence similarity.