title: 48S rDNA of marine sediments
design: The V4 region of bacterial 16S rRNA genes was amplified using universal primers 515F and 806R. All PCR reactions were carried out with 15 L of Phusion High-Fidelity PCR Master Mix (New England Biolabs). Mix same volume of 1X loading buffer (contained SYB green) with PCR products and operate electrophoresis on 34% agarose gel for detection. Then, mixture PCR products was purified with Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated using TruSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) following manufacturer's recommendations and index codes were added.