title: GSM5742672: RNA-Seq: keloid fibroblasts with blank plasmid knock-down; Homo sapiens; RNA-Seq
library_construction_protocol: Three anonymous patients affected with chronic keloids underwent keloid excision surgery. Keloid explants were collected from each patient under aseptic conditions. It was flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols