title: Whole blood RNAseq of tuberculosis patient infected with MTBC L4
design: Study of whole blood transcriptional profile in TB patients infected with MTBC lineage-specific bacteria (L4 infection Patient #13). Blood samples were collected into Tempus Blood RNA Tubes and stored at -80C until further use. RNA was extracted with Tempus Spin RNA Isolation Kit and globin mRNA depleted using the human GLOBINclear Kit. The sequencing library was prepared using the Nextera kit and sequenced using 75bp single-end Illumina NextSeq500 technology.