title: GSM5253079: Total RNA MDA CRISPRI Ile GAT OE Ile TAT R1; Homo sapiens; OTHER
library_construction_protocol: Cells flash frozen in Liquid Nitrogen, Lysis buffer (20mM Tris pH 7.4, 150mM NaCl, 5mM MgCl2, 1mM DTT, 100ug/ml Cycloheximide, 1% Triton X-100, 25U/ml Turbo DNaseI); RNA extracted in Trizol Standard Illumina RNA library prep