title: GSM4980428: Male aged Rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Bone endothelial cells were purified using endomucin expression  and sorted directly into lysis buffer of the RNeasy Plus Micro Kit (QIAGEN). Total RNA was isolated as per the manufacturer's guidelines. RNA quality was checked using a 2100 BioAnalyzer (Agilent), and libraries were prepared with TruSeq Total RNA Library Preparation Kit (Illumina) following the manufacturer's guidelines. Sequencing of libraries was carried out at the LMS Genomics Facility on an Illumina HiSeq 2500 platform with 100-nucleotide-length paired-end reads.