title: GSM6138595: Citrus unshiu-normal-shoots [Bisulfite-Seq]; Citrus unshiu; Bisulfite-Seq
library_construction_protocol: Total DNA was extracted from shoots using the Hi-DNAsecure Plant Kit (Tiangen Biotech Co., Ltd. Beijing, Cat. No. DP350) following the instructor's manual. The conversion of unmethylated cytosines to thymidines of 1 µg fragmented DNA was carried out using the EZ DNA Methylation-Gold™ Kit (Zymo Research, Cat. No. D5005) following the instructor's manual. The Sequencing libraries were prepared using the Accel-NGS® Methyl-Seq DNA Library kit (Swift BiosciencesTM, Cat. No. 30024), and PCR products corresponding to 300–500 bp were enriched, and quantified, and then finally sequenced on NovaSeq 6000 (Illumina).