title: Illumina HiSeq 2500 paired end sequencing; Functional analyses of human LUC7-like proteins involved in splicing regulation and myeloid neoplasms
library_construction_protocol: RNA was isolated from LUC7-like knockdown and control cell lines as follows. Cells (6-7 million) were collected, pelleted, and kept on ice. High Pure RNA Isolation Kit (Roche) protocol was used following manufactures instructions to purify the RNA.  K562 cells were grown in RPMI, 10%  FBS, and 100 U/mL penicillin-streptomycin. shRNAs were delivered to K562 cells via lentivirus to deplete the LUC7-like protein of interest RNA was isolated from LUC7-like knockdown and control cell lines as follows. Cells (6-7 million) were collected, pelleted, and kept on ice. High Pure RNA Isolation Kit (Roche, 11828665001) protocol was used following manufacturerâ€™s instructions to purify the RNA.  Ribosomal RNA was depleted using the Illumina RiboZero Plus kit and libraries were prepared for high throughput sequencing using the Illumina TruSeq kit according to manufacturer's protocols.