title: GSM4801423: Osx-Cre;NT3 #18; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were excised to remove any gross overlaying tissue, and either stored at -20C in RNAlater (R0901; Sigma, USA) or flash frozen in liquid nitrogen, and stored at -80C.  Frozen tumors were pulverized in liquid nitrogen using a Mikro Dismembrator (Mikro‐Dismembrator S; B. Braun Biotech International, Melsungen, Germany), resuspended in TRIzol (15596026; Invitrogen, Carlsbad, CA, USA), and stored at –80°C. Total RNA was isolated using a Direct‐zol kit per the manufacturer's instructions (R2072; Zymo Research, Irvine, CA, USA). mRNA version 2 library prep kit (Illumina)