title: GSM5252995: Total RNA MDA CRISPRI Ile GAT Ctrl B; Homo sapiens; OTHER
library_construction_protocol: 425uL Lysis Buffer (5mM Tris HCl pH 7.5, 2.5mM MgCl2, 1.5mM KCL, 100ug/mL cycloheximide, 2mM DTT, 0.5% Triton X-100, 0.5% sodium deoxycholate, 100units SUPERase*In Rnase Inhibitor, 1x Protease Inhibitors EDTA-free); RNA extracted with 3:1 Trizol LS Reagent after polysome fractioning Lexogen QuantSeq 3' mRNA-Seq Library Prep kit, 500ng RNA used as Input