title: GSM5386738: 8505C-Par-UT-2; Homo sapiens; RNA-Seq
library_construction_protocol: 1 μg of total RNA was subjected to library synthesis using the TruSeq RNA-Seq kit. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR. Sequencing was performed using 12pM hybridization to a 1x51 end flow cell. RNA libraries were prepared for sequencing using standard Illumina protocols. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.