title: GSM6045699: ATAC-seq in Lola-I mutant ORC4 17-20hrs embryo Rep1; Drosophila melanogaster; ATAC-seq
library_construction_protocol: Nuclei were isolated from Oregon-R embryos 2-4h AED by resuspending and douncing embryos in HBS buffer (0.125 M Sucrose, 15 mM Tris (pH 7.5), 15 mM NaCl, 40 mM KCl, 2 mM EDTA, 0.5 mM EGTA). Isolated nuclei were treated with Tn5 transposase((Illumina, #FC-121-1030). Nextera index kit (Illumina, #FC-121-1011) was used to create libraries.