title: GSM5334795: Wistar_SON_dehydrated_rep5; Rattus norvegicus; RNA-Seq
library_construction_protocol: All rats were humanely euthanised by striking of the cranium (stunning), and then immediately decapitated with a small animal guillotine. Brains were rapidly removed from the cranium and placed into a chilled rat brain matrix for separation of the forebrain from the hindbrain. The forebrain was placed cut edge down onto aluminium foil resting on pellets of dry ice and covered with powdered dry ice (within 3 minutes of stunning). By using a cryostat, bilateral punches of the SON were collected from 12 coronal slices with a 1 mm sample corer.  Animal experiments were performed between 10 am-12 pm. Illumina TruSeq Stranded mRNA Library prep (Cat. no. 20020594) following manufacturer's protocol.