title: GSM6217217: T3 10nM 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen, Carlsbad, CA, USA). RNA samples were quantified in Qubit and the integrity of the RNA was analyzed using a  Bioanalyzer 2100® (Agilent, Santa Clara, CA, USA). Three RNA samples from each group were used to build each of the libraries. The libraries were constructed using the TruSeq1 RNA Sample Prep kit v2 (Illumina, San Diego, CA, USA) following the protocol suggested by the manufacturer. Runs were performed on the Illumina HiSeq2500 platform with 100 bp paired end reads.