title: GSM5729296: amos mutant larval antennal disc 1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For mRNA extraction, both males and females were used. Eye-antennal discs were dissected from third instar larvae into ice-cold Schneider's Drosophila medium (Life Technologies). 50 antennal discs were manually separated from the eye disc and immediately placed into 300 μl Trizol (Invitrogen). Adult antennae were collected from 0-4 h old flies by quickly freezing the animals in dry ice and separating the antennae from other tissues by shaking through a 20 μm sieve. Approximately 300 antennae were transferred into a 1.5 ml Eppendorf tube containing 20 μl Trizol. The antennae were homogenized with an RNase-free pestle until no intact cuticle could be detected, and the lysate was transferred into 300 μl Trizol. RNA purification was performed following a standard protocol (PMID:20516177) followed by column purification (RNeasy, QIAGEN). RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA protocol