title: GSM5253168: DNase-seq_Vitro_Th17-2; Mus musculus; DNase-Hypersensitivity
library_construction_protocol: Detailed procedures for DNase-Seq (in duplicates) were reported previously (Jin et al., 2015). Dnase and ChIP DNA were end-repaired using an End-It DNA End-Repair kit (Epicentre), universal adaptors were then ligated. The libraries were amplified using indexed primers with the PCR condition: 98°C, 10”; 67°C, 30”; 72°C, 30” for 16 cycles. The fragments between 180bp to 350bp were isolated on E-gel and subjected to sequencing on Illumina instruments.