title: GSM5253205: 48h_1299_4_Scr_1uM_PTC; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with RNA/DNA/Protein Purification Plus kit (Norgen), polyA mRNA was selected and library prepared using the NEBNext Ultra II Directional RNA Library Prep kit. Libraries were size selected, multiplexed and 75bp paired-end sequences were obtained with HiSeq4000 (Illumina)