title: GSM4802472: XBN3; Danio rerio; RNA-Seq
library_construction_protocol: Cells were FACS sorted into TRIZOL LS and snap-frozen. Total RNA was extracted using TRIZOL LS protocol. Total RNA varied from 1.2ng to 150ng, all having RIN scores > 7. Reverse transcription and cDNA amplification were performed with SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were constructed using Illumina Nextera XT kit and analyzed for concentration (Qubit), size distribribution (Agilent Bioanalyzer).