title: GSM6213396: GMP, Control, replicate 2; Mus musculus; ATAC-seq
library_construction_protocol: Sorted cells were pelleted and then lysed in mild lysis buffer (10mM Nacl, 10mM Tris-HSC, 0.5% NP-40, Rnase inhibitor) for 30 minutes. Cells were vortexed for 1 minute and centrifuged at 2000 g for 5 minutes at 4oC. The lysis supernatant was removed, then the transposase reaction mix (Transposase enzyme, buffer, and digitonin) was added to the pelleted nuclei and incubated for 30 minutes at 37oC. DNA was then purified and cleaned. ATAC-seq libraries were prepared by amplifying the DNA fragments using barcoded primers and the NEBNext HF PCR kit. The samples were amplified on a thermocycler for 10-11 cycles depending on amplification efficiency determined using qRT-PCR. Libraries were validated using the Agilent D1000 ScreenTape system.