title: GSM4852653: Exp2_shHSPB3_Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: For gene expression analysis on LHCN-M2 cells, total RNA was isolated using Trizol (R2050; Zymo Research, USA), as per manufacturer's instructions. Followed by purification using RNA Clean & Concentrator (R1017; Zymo Research, USA) and DNase I Set (E1010; Zymo Research, USA), as per manufacturer's instructions. Library Preparation was done using TruSeq Stranded mRNA, according to manufactures protocol.