title: GSM4802191: Mouse1_MC38_tumor_CD8Tcells_VDJ; Mus musculus; RNA-Seq
library_construction_protocol: Sorted CD8+ CD44+ T cells from peripheral blood and sorted CD8+ T cells from tumors were loaded into the 10X Chromium Controller using the Chromium Single Cell 5' Library and Gel Bead Kit (10X Genomics) For samples with gene expression and T cell receptor sequencing only, protocol number CG000086 Rev G from 10X Genomics (User Guide, Chromium Single Cell V(D)J Reagent Kits) was used. For samples with had gene expression, T cell receptor sequencing, and CITE seq to detect Feature Barcodes, protocol number CG000208 RevD from 10X Genomics (User Guide, Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 with Feature Barcoding technology for Cell Surface Protein) was used 10X Chromium Single Cell V(D)J kits (5' gene expression with paired T cell receptor sequencing, and for some samples Feature Barcode detection)