title: GSM5728741: Animal 13669, entorhinal cortex, index  TTAGGC, 1st run [B4_EC]; Papio hamadryas; miRNA-Seq
library_construction_protocol: Baboons were sedated with ketamine (10mg/kg) prior to collection of blood in Cell Preparation Tubes (Becton-Dickinson, Franklin Lakes, NJ, USA), anesthetized (1-2% isoflurane), and euthanized via either intravenous injection of pentobarbital (>100mg/kg) or exsanguination while under general anesthesia. Necropsy was performed to obtain brain tissue. PBMCs were isolated according to the manufacturers' protocol. Brain regions were dissected from fresh tissue, placed in a cryovial, and flash frozen in liquid nitrogen. All samples were stored at -80°C. Total RNA was isolated from PBMCs and brain regions using the miRNeasy Mini Kit (Qiagen, Germantown, MD). Small RNA sequencing libraries were prepared using the Illumina Truseq Small RNA Sample Prep Kit.