title: GSM4802686: ChIP_sgBRD8_H2AZ; Homo sapiens; ChIP-Seq
library_construction_protocol: Cell lysates were clarified from sonicated nulcei; BRD8, p53, and histone-DNA complexes were isolated with Flag or histone antibody. Libraries were constructed using Illumina TruSeq ChIP Sample Prep kit following manufacture's protocol. Briefly, 50 µl of ChIP DNA was end-repaired, 3'-adenyl tailed, and then ligated with different index adaptors. The adaptor-ligated DNA was size selected (250-300 bp) using agarose gel electrophoresis, followed by PCR amplification. The amplified DNA was purified with AMPure XP beads (Beckman Coulter).