title: GSM4802006: STIM1 KO_KO1 (Replicate1); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the purkinje neuronal layer alongwith with the molecular layer using TRIzol as per manufacturer's instructions. The isolated RNA was run on a Bio-analyzer chip to ensure integrity. Approximately 500 ng of total isolated RNA with an average RIN of 8.2 was used per sample to prepare libraries using TruSeq mRNA Library Prep Kit following manufacturer's instructions. The prepared libraries were run on a DNA chip in the bio-analyzer to check for their size followed by its quantification using qPCR. The libraries were then run on an Illumina Hiseq 2500 platform using single end and 50 bp read protocol (NGS Facility, NCBS).