title: GSM4802448: FAXINO80KO256U; Mus musculus; MNase-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes at room temperature. Chromatin was fragmented by MNase digestion. 4, 16, 64 or 256 U of MNase (NEB) were added to per 2M pre-warmed cells and incubated at 37 °C for 10 min. Digestion was halted by addition of MNase stop buffer (20mM EDTA, 20mM EGTA, 0.4% SDS and 0.5mg/ml Proteinase K). The mixtures were incubated at 65 °C for 60 min to reverse crosslinks. DNA was cleaned up by Gel Purification Column (Qiagen). 100 ng DNA from each digestion were used to prepare DNA libraries as described in the Nextflex Rapid DNA-seq Kit (PerkinEImer).  Size selection was performed after adaptor ligation with Agencourt AMPure XP beads (Beckman Coulter)