title: GSM5215330: SP mRNA-Seq DbhVLM-mCherry replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of CD45+ cells was extracted using a Takara MiniBest Universal RNA Extraction kit (code no. 9767) according to the manufacturer's instructions. Poly(A) mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB,E7490L). poly(A) mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB,E7490L).First-strand cDNA was synthesized with the SuperScript II Reverse Transcriptase (Invitrogen,18064014) and Second Strand cDNA was synthesized using Second Strand cDNA Synthesis Kit (Invitrogen,A48570). Libraries were subsequently constructed with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB,E7645L).