title: GSM6217097: Seedlings, c3c4c9, rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated using the E.Z.N.A. Plant RNA Kit from Omega Biotek. To ensure low levels of contaminating rRNA, two rounds of poly(A) mRNA purification were performed using the µMACS mRNA Isolation Kit (Miltenyi Biotech). Indexed RNA-seq libraries were prepared from 100 ng of poly(A) RNA starting material using the NEXTflex Illumina qRNA-Seq Library Prep Kit (Bioo Scientific; catalog no. 5130). Sequencing of 150-bp paired-end reads was performed on an Illumina HiSeq 2500 in rapid output mode at the Texas A&M Agrilife Research Facility (College Station, TX). Sample demultiplexing was performed using CASAVA software v1.8.2, and bcl2fastq was performed using conversion software v1.8.4.