title: GSM5574424: M10-AM-Z3--HC; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from alveolar macrophages was extracted using Qiagen RNeasy Plus Mini kit (Qiagen) and quality and quantity were assessed 4200 TapeStation  Agilent RNA-Seq libraries were prepared using a full-length transcript coverage NEBNext Ultra RNA (New England Biolabs) DNA libraries sequencing was performed using an Illumina NextSeq 500 instrument (Illumina) of the average dept of 5–10 million aligned reads per sample