title: GSM5335344: DNA_4RS3-BS: Articular cartilage right leg rep3 week 4; Mus musculus; OTHER
library_construction_protocol: DNA was isolated using the Gentra Puregene DNA isolation kit (Qiagen) Briefly, 200 ng of genomic DNA were digested with MspI (New England Biolabs, Ipswich, MA), and cleaned up using QIAquick PCR purification columns (Hilden, Germany). Six uniquely indexed libraries were generated using the Ovation Ultralow Methyl-Seq DR Multiplex with TrueMethyl oxBS kit and workflow (Tecan, Redwood, CA). All six libraries were pooled together and divided into two parts, one part was subjected to oxidation and bisulfite conversion (oxBS), the other part was mock oxidized, and subjected to bisulfite conversion (BS). The library pool for BS was amplified for 7 cycles and oxBS for 9 cycles. After amplification library pools were subjected to sequencing.