title: GSM6199003: Hypothalamus, active, animal 4 [4_HT_A]; Ovis aries; RNA-Seq
library_construction_protocol: Samples from HT, PT and PG tissues were isolated and rapidly frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted from tissue samples using RNeasy Lipid Tissue mini kit (QIAGEN, Madrid, Spain) according to the manufacturer's instructions Sequencing was carried out at the CNAG (Centro Nacional de Análisis Genómico, Spain; https://www.cnag.crg.eu/). The RNA-Seq library was prepared with KAPA Stranded mRNA-Seq Illumina Platforms Kit (Roche) following the manufacturer´s recommendations using Illumina platform compatible adaptors with unique dual indexes and unique molecular identifiers (Integrated DNA Technologies). The final library was validated on an Agilent 2100 Bioanalyzer with the DNA 7500 assay. The libraries were sequenced on NovaSeq 6000 (Illumina) with a read length of 2x151bp following the manufacturer's protocol for dual indexing.