title: GSM4801744: ncRNA-HI4-GFP; Homo sapiens; ncRNA-Seq
library_construction_protocol: The human islets were lysed with 350 uL of QIAzol Lysis Reagent (Qiagen) and stored at -80˚C before purifying RNA using Mini RNeasy purification kits per manufacturer's instructions. Total RNA was treated with RiboZero purification and oligo-dT depletion in order to enrich for ncRNAs. RNA Barcoded Libraries were prepared according to Illumina's instructions (2013) accompanying the TruSeq RNA Sample prep kit v2 (Part# RS‐122‐2001).