title: GSM6219595: 414T; Canis lupus familiaris; OTHER
library_construction_protocol: Tissues were simultaneously flash frozen and OCT embedded according to 10x Genomics demonstrated protocol CG000240 Libraries were prepared according to manufacturer's protocol (10x Genomics demonstrated protocol CG000239). Briefly, 10um tissue sections were placed onto capture areas of visium slides and stained with hematoxylin and eosin, imaged, then permeabilized for 18 minutes (murine) or 24 minutes (canine). cDNAs were synthesized and amplified, followed by library construction.