title: GSM4781985: P0y110 5; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Snap frozen plant rosettes were ground, DNA extraction was done using Zymo ZR Plant/Seed DNA microprep kit according to the manufactorer's protocol Three different treatments (γ0 = control, γ30 = 30 mGy/h, γ110 = 110 mGy/h) per generation (P0, S1, S2) were selected for sequencing. This resulted in a total of nine different conditions with 5 biological replicates for each condition. Concentration of the extracted DNA was measured using the 'Quant-it Picogreen dsDNA assay kit' (Life Technologies, Grand Island, NY, USA). Subsequently, 400-600 ng of gDNA was fragmented to 300bp using the Covaris S2 focused-ultrasonicator (Covaris, Woburn, Massachusetts, USA). The size of the fragmented DNA was checked on a High sensitivity DNA chip (Agilent Technologies, Santa Clara, CA, USA). Library preparation with NEBNext Ultra II DNA library prep kit (New England Biolabs, Ipswich, MA, USA) was performed using methylated adapters, according to the manufacturer's protocol.