title: GSM5287870: 4hr Glucan 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated under an RNase-free environment, using the RNeasy Mini Kit (Qiagen, Hilden, Germany), and treated with DNase (Qiagen). Total RNA quality and concentration were verified with a Thermo Scientific NanoDrop 2000 spectrophotometer. mRNA Libraries were prepared using NEBNext Poly(A) selection (New England Biolabs). Sequencing was performed at paired-end 150 bp on an Illumina NovaSeq 6000 with at least 50 million reads per sample by Vanderbilt Technologies for Advanced Genomics