title: GSM4802323: miRNA-seq_HIF_3a_2; Homo sapiens; miRNA-Seq
library_construction_protocol: A total of 3μg of total RNA was used for small RNA cDNA library preparation with Balancer NGS Library Preparation Kit for small/microRNA (GnomeGen) following manufacture's instruction. Briefly, RNAs were ligated to 3' and 5' adaptor sequentially and reverse transcribed to cDNA and then PCR amplified. Whole library was applied to 10% native PAGE gel electrophoresis and ba nds corresponding to microRNA insertion were cut and eluted. After ethanol precipitation and washing, the purified small RNA libraries were quantified and stored at -80 ℃ before sequencing For high-throughput sequencing, the libraries were prepared following the manufacture's instructions and applied to illumina NextSeq 500 system with 150x2 paired-end type (ABLife Inc., Wuhan, China).