title: GSM5335174: KL_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Lung tumor samples were freshly dissected and preserved in RNAlater, and RNA was extracted using RNeasy Mini Kit (QIAGEN) and treated with DNase. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. mRNA-seq libraries were prepared with the SureSelect Strand Specific RNA library prep kit (Agilent Technologies) after PolyA capture, and then sequenced by single-end 75 base pairs using the Illumina NextSeq 500 platform.