title: GSM4801947: E20180629_hypertrophic cardiomyocyte-derived exosomes; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Total exosomal RNA was extracted with Trizol. The Illumina TrueSeq small RNA library prep kit was then used to construct a small RNA library from exosomal RNA obtained. Adapters were directly, and specifically, ligated to the 3′ and 5′ end of the RNA. The ligated RNA molecule was reverse transcribed into single-stranded cDNA for PCR amplification. After that, sequencing was then performed on an Illumina HiSeq 2000 sequencer.