title: GSM6222393: nomal-4; Homo sapiens; RNA-Seq
library_construction_protocol: The normal tissues was 2cm adjacent to the carcinoma. Cancer tissues was confirmed by postoperative pathology as papillary thyroid carcinoma. The patients' cancer tissues and normal paracancer tissues were collected and stored in the refrigerator at -80℃ for long-term storage for high-throughput sequencing. A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly,ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.