title: GSM4981570: 415B_TNF: CAGE-seq, Primary FLS, TNF stimulated; Homo sapiens; RNA-Seq
library_construction_protocol: Cultured human FLS from RA patients were treated with 10 ng/ml TNF, 24h or were left untreated (Supplementary table 2). RNA was isolated using the Quick RNA MicroPrep Kit (Zymo Research). CAGE libraries were prepared and sequenced as previously described in Kodzius et al.