title: GSM5994522: THP1 cells stably expressing scrambled siRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNAzol reagent. Eluted RNA was treated with turbo Dnase and purified with RNeasy mini kit from Qiagen. 1 μg of total RNA was used library preparation. Truseq RNA Library Prep Kit protocol was used to prepare the libraries for total RNA sequencing. Briefly mRNAs were fragmented in fragmentation buffer after which cDNA was synthesized using oligo-dT as primers. The complementary strand of the cDNA was synthesized using DNA polymerase I, in dNTPs mixture dTTP was replaced with dUTP. The double stranded DNA was purified using AMPureXP beads. Then the dUTP containing strands were degraded by USER Enzyme, and strands were amplified using PCR and purified using AMPure XP beads. These cDNA libraries used for sequencing.