title: GSM4981534: SF_346_TNF: RNA-seq, Primary FLS, TNF stimulated; Homo sapiens; RNA-Seq
library_construction_protocol: Cultured FLS were treated with 10 ng/ml human recombinant TNF (Roche) for 24 h or were left untreated. Total RNA was isolated using the RNeasy Mini kit (Qiagen) including on-column DNAase I digestion. RNA-seq libraries were prepared using the NEB Next Ultra Directional RNAseq Protocol with ribosomal depletion.