title: Illumina HiSeq 2500 sequencing; Epigenetic regulation during the pathologic process from human primary Sjögren's syndrome to mucosa-associated lymphoid tissue lymphoma
library_construction_protocol: Tissues were captured from two patients, and stored at -80oC immediately. In brief, 10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of H3K4/9/27/36/79me3 (CST, Boston, USA) were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 4 °C for target protein pull down. The DNA pulled down or input DNA were repaired to 3'-dA overhang and added the ligated adapter.