title: GSM5384528: HEKs, RPRD1B_KD; Saccharomyces cerevisiae; Homo sapiens; OTHER
library_construction_protocol: The total RNA extractions were performed with TRIzol  according to the manufacturers' instructions. 4-thiouracil-labelled S.Cerevisiae RNA was used as spike-in. biotinylation was done for 4Su/4TU labeled residues of RNAs. Then biotylated RNAs were eluted  with Dynabeads™ M-270 Streptavidin RNA libraries were prepared for sequencing using standard Illumina protocols