title: GSM6262394: Kidney_WT_H3K9ac_Veh_rep6; Mus musculus; ChIP-Seq
library_construction_protocol: The kidneys were isolated, rinsed with cold 1× phosphate-buffered saline, and subjected to a cross-linking reaction for 10 min with 1.5% formaldehyde. After the fixative was neutralized for 5 min with glycine (0.125 m final concentration), the kidney tissue was homogenized briefly in phosphate-buffered saline using a loose fitting Dounce pestle, and the cells were collected via 70-μm nylon cell strainers (BD Falcon). Cells were then subjected to ChIP analysis as described previously (Yamamoto H, 2003, Journal Biol Chem). ChIP-seq libraries were prepared using the NEBNext Ultra II DNA kit (NEB, #E7645S) with the NEBNext Multiplex Oligos for Illumina (NEB, #E6440S) according to the manufacturer's protocols. Libraries were submitted to the University of Wisconsin – Madison Biotechnology Center's DNA Sequencing Facility (Research Resource Identifier – RRID:SCR_017759). Libraries were sequenced on a NovaSeq 6000. Paired end, 250 bp sequencing with a target of 20+ million reads was performed.