title: GSM5253158: H295R_AngII_control_0_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: H295R cells were washed and treated with TRIzol and RNA was extracted using ZYMO DirectZol RNA Miniprep kit. rRNA was depleted using ribozero gold; RNA from human adrenocortical cells was polyA-selected. For samples treated with 4-thiouridine, labeled RNA was biotinylated and purified using streptavidin as described previously (Methods Mol Biol. 2013;1064:91-100. doi: 10.1007/978-1-62703-601-6_6). All RNA was made into cDNA libraries using Kappa HyperPrep kit an sequenced on Nova-seq.