title: GSM5336263: Root-non_inoculated-control-2 [R-NI-C-2]; Populus trichocarpa; RNA-Seq
library_construction_protocol: Total RNA of each sample was extracted separately. The RNA extraction was performed using the RNeasy Plant mini kit (Qiagen, Courtaboeuf, France) per the manufacturer's instructions, with the addition of 25 mg of polyethylene glycol 8000 per milliliter of RLC buffer (RNeasy, Qiagen). A DNA digestion step with DNAse I (Ambion, Invitrogen) was also included to remove DNA contamination. RNA quality was verified by Nanodrop (Thermo Fisher Scientific) and Fragment Analyzer (Agilent, Santa Clara, USA). Stranded cDNA sequencing libraries were generated from 1-3 µg of total pooled RNA following the manufacturer's instructions (Illumina Inc., USA) followed by Illumina HiSeq 3000 mRNA sequencing   (RNA-Seq) at the Genotoul sequencing facilities (Toulouse, France).