title: GSM5066757: T3; Bos taurus; RNA-Seq
library_construction_protocol: four whole blood samples included either no LPS treatment - control sample CO, or three treated samples with LPS (2 μg/ml, Product Number: L2880, Sigma Aldrich, Saint Louis, MO, USA) for 2 hours - T1, 4hours - T2, and 8 hours - T3 at 37°C. PBMCs were isolated by centrifugation of whole blood on Hanks' Balanced Salt Solution (Solarbio; Beijing, China) at 500 g for 20 min at roomtemperature. scRNA-seq Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3′ Reagent Kits v3 User Guide: CG000183 Rev C.