title: GSM5833214: exon 7 of LRRFIP2 variant 3-deleted MKN1 cell line #2 [MKN1_ko_2]; Homo sapiens; RNA-Seq
library_construction_protocol: The cDNA libraries were prepared from Total RNA for 151bp paired-end sequencing using TruSeq stranded mRNA Sample Preparation Kit (Illumina). Total RNA from each cell for RNA sequencing was isolated using TRIzol reagent following the manufacturer's instructions. The mRNA molecules were purified and fragmented from 1ug of Total RNA using oligo (dT) magnetic beads. After sequential process, cDNA libraries were amplified with PCR (Polymerase Chain Reaction) and were subsequently examined for quality using an Agilent 2100 bioanalyzer (Agilent). They were quantified with the KAPA library quantification kit (Kapa Biosystems) according to the manufacturer's protocol.