title: GSM5288067: RNA-seq_AB-I-LL-2; Oenothera elata subsp. hookeri x Oenothera grandiflora; RNA-Seq
library_construction_protocol: Total RNA isolations from evening primroses were performed employing protocols specially developed for their mucilage and phenolic compound rich tissue, described in Massouh et al. 2016, Plant Cell 28: 911-929. For RNA-seq analyses, total RNA was purified from residual DNA contamination by digestion employing the Ambion® Turbo DNA-freeTM Kit. monosome, polysome, and ribosome footprint isolation: The procedure for monosome, polysome, and ribosome footprint isolation are precisely described in Zupok et al. (2021) A photosynthesis operon in the chloroplast genome drives speciation in evening primroses. Plant Cell, in press. Ribo-seq libraries were prepared using the NEXTflex Small RNA-Seq Kit v3. RNA-seq libraries were prepared using the Zymo Research Zymo-Seq RiboFree Total RNA Library Kit.