title: Illumina HiSeq 2000 paired end sequencing; RNA-seq of Oryza sativa L. Dongjin and Iksan 526 seeds at 20 days after heading
library_construction_protocol: Developing rice (Oryza sativa subsp. japonica) seeds, Dongjin and Iksan 526, respectively, were harvested from one panicle per plant (total four plants per replication) at 6, 13, 20, 31 and 41 days after heading (DAH). Seedlings of wild-type rice (Oryza sativa subsp. japonica cv. Dongjin) and an inbred line of its transgenic counterpart, Iksan 526 (I.526), were used in this study. The seedlings were transplanted in a genetically modified organism (GMO) paddy field (30 cm × 15 cm) of the National Institute of Crop Science (NICS), Republic of Korea, using the standard rice cultivation method of the NICS. A completely randomized design (CRD) was used, with three replications. Total RNA was extracted from Dongjin and Iksan 526 seeds at 20 days after heading using the RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer's instructions.  TruSeq RNA Sample Prep Kit v2 was used for library construction.