title: GSM4983068: dCAPH2_clone211_Dam-Lamin_rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was isolated using the Bioline Isolate II Genomic DNA Kit. The full protocol is described in Leemans, 2019, with the addition of a dephosphase step to prevent amplification of apoptotic fragments. 500 ng of genomic DNA was first dephosphorylated with rSAP (NEB), before digested at Dam-methylated GATC sequences with DpnI and ligated to adapters. Following DpnII digestion of intact GATC sequences, DNA fragments were amplified with 22-26 PCR cycles. Amplified fragments were further processed with end-repair, 3A-overhang tailing and Y-shaped adapter ligation, before a final round of 9-12 PCR cycles with illumina index adapters.