title: GSM4852505: ES FCS_Rep 2_SA1 3p KD; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was first depleted of rRNA (ES FCS) or enriched for PolyA (ES 2i). Two rounds of rRNA depletion were done. RNA-seq libraries were prepared using NEBNext Ultra directional RNAseq kit with 8-10 cycles of PCR.