title: GSM5386734: Endo_H3N2_rep_1; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA extraction, chips were removed from pods. An empty 200 µL filtered tip was inserted to top channel outlet and washed with 100 µL DPBS (+/+) into the top channel inlet to wash the apical channel. After collecting top channel washes, a new empty 200 µL filtered tip was inserted to top channel outlet and 100 ul RNase easy lysis buffer (Qiagen, #74034) was used to lyse epithelial cells by quickly pressing and releasing the micropipette plunger 3 times. Lysates were collected in a clean labelled 1.5 ml tube and stored at -80˚C for RNA-seq analysis. RNA libraries were prepared for sequencing using standard Illumina HiSeq with 150-bp pair-ended reads