title: Illumina NovaSeq 6000 sequencing; Mapping immune development across tissues in the human fetus - 10X spatial transcriptomics
library_construction_protocol: OCT embedded fresh frozen samples were used for 10x Genomics Visium, and samples were processed following manufacturer's instructions. All tissues were sectioned with a thickness of 15 µm. Tissue optimisation was then performed with 18-min permeabilisation time for fetal spleen and liver, and 24-min for fetal thymus. The spatial gene expression library was then generated following the manufacturer's protocol (Visium 10X genomics). [image acquisition for Visium 10X] All tissues were sectioned with a thickness of 15 µm. Tissue optimisation was then performed with 18-min permeabilisation time for fetal spleen and liver, and 24-min for fetal thymus. The spatial gene expression library was then generated following the manufacturer's protocol. All images for this process were scanned using a Zeiss AxioImager system.   The spatial gene expression library was then generated following the manufacturer's protocol (Visium 10X genomics).