title: GSM5145651: 4C_PromSix2VP_inv110; Mus musculus; OTHER
library_construction_protocol: Circular Chromatin Conformation Capture (4C) followed by sequencingwere performed as previously described (Laugsch et al., 2019). 4C samples were amplified by inverse PCR using primer for the PE Sox1, PE Six3 or Six2 promoter. The primer included illumina adaptor sequences and short barcodes. The inverse PCRs were performed with the expand long template PCR system and 30 amplification cycles (94°C 2 min, 30x [94°C 10 sec, 60°C 1 min, 68°C 3 min], 68°C 5 min). The inverse PCR primers were designed as previously described (Stadhouders et al., 2013). Once amplified, 4C-seq libraries were sequenced on the HiSeq2500.