title: GSM6005025: 4-hydroxytamoxifen resistant T47D cells - treated with 4-OHT - rep_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cell lines with an RNeasy isolation kit (Qiagen) according to the manufacturer's protocol (Life Technologies). A NanoDrop™ 2000 spectrophotometer (Thermo Fisher Scientific), an Agilent TapeStation system (Agilent, Santa Clara, CA) and a Qubit fluorometer (Thermo Fisher Scientific) were used to assess the quality and quantity of extracted RNA. Sequencing and library preparation were performed by the Norwegian Sequencing Centre (University of Oslo, Norway). Libraries were prepared using 100 ng total RNA as input material for the TruSeq Stranded RNA Library Prep Kit (Illumina) and standard Illumina adaptors for multiplexing. Libraries for each individual sample were sequenced using the NovaSeq SP Illumina platform, creating paired-end 150 bp reads with an average library size of 650 million reads.