title: GSM5384978: 6_CUTnTag_2cell_Nr5a2_rep2; Mus musculus; OTHER
library_construction_protocol: CUT&Tag was performed as described previously with a few modifications (Kaya-Okur, H.S., Wu, S.J., Codomo, C.A. et al. CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun 10, 1930 (2019)). Cells with intact zona pellucida were pre-extracted and lightly fixed by DPBS with 0.1% formaldehyde for 2 min at room temperature. Tagmentation of chromatin bound by antibodies and pA-Tn5 was done in wash buffer with either low (150 mM NaCl) or high (300 mM NaCl) salt condition. Extracted DNA was amplified for 15 PCR cycles, then, libraries were purified by 1.1xAMPure XP magnetic beads (Beckman Coulter) and were eluted in 20 µl of RNase-free water.