title: GSM6222345: 22siControl-MDA-MB-231; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using RNeasy Mini Kit (Catalog Nos; 74104, 74106 Qiagen) according to the manufacturer's instructions. Libraries were prepared using the INCPM-mRNA-seq protocol. Briefly, the polyA fraction was purified from the 500ng of total RNA, followed by fragmentation and generation of double-stranded cDNA, Agencourt Ampure XP beads cleanup (Beckman Coulter, USA), end repair, A base addition, adapter ligation, and PCR amplification.