title: Illumina HiSeq 2500 paired end sequencing; Epigenetic regulation during the pathologic process from human primary Sjögren's syndrome to mucosa-associated lymphoid tissue lymphoma
library_construction_protocol: Tissues were captured from two patients, and stored at -80oC immediately. Total RNA of tissues containing 5x10^5 was extracted using Trizol (Thermo Fisher Scientific, USA) according to the manufacturer's protocol. 5 μg of RNA in each group were used for library preparation by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations.