title: GSM4851878: mESC_Chr_rep 1_Small; Mus musculus; miRNA-Seq
library_construction_protocol: Total RNA was collected with the Trizol LE regent for cytoplasmic RNAs, and Trizol reagent for soluble nucleoplasmic and chromatin-associated RNAs. Short RNA below 200 nt was selected using RNeasy MinElute Cleanup Kit (Qiagen). RNA was processed with Ribo-minus kit to remove ribosomal RNA. Libraries were constructed with the Illumina TruSeq Small RNA sample Prep Kit. A final library of 120-160 bp was size selection with an TBE gel.