title: GSM4981628: PA3027 overproducer (PA3027), biological rep2; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: Total RNA was isolated from three independent replicates of each strain. 2 ml of cultures which reached optical density 0.5 at 600 nm were mixed with 4 ml of RNAprotect Bacteria Reagent (Qiagen). RNA was isolated with an RNeasy mini-kit (Qiagen) according to the manufacturer's protocol. Total RNA was digested with DNase (TURBO DNA-free Kit, Ambion) to eliminate genomic DNA. Ribosomal RNA was depleted using RiboZero Bacteria kit (Illumina). Obtained mRNA was used for cDNA library construction using KAPA Stranded RNASeq kit. Library was further quality checked on 1% agarose gel and concentration was measured using qPCR KAPA Library Quantification Kit.