title: GSM5907073: yeast cells, H10355 rep3; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: 1.5 ml of meiotic culture was collected for each sample. RNeasy Mini Kit (Qiagen) was used for total RNA extraction following the kit`s instructions for mechanical extraction.  We assesed the quality of RNAs with Tapestation 2200. We started with 1.2 µg of total RNA for library preparation. polyA enrichment was perfomed using Sera-Mag oligo(dt) Magnetic particles (Sigma-Aldrich). cDNA synthesis was achieved in similar manner to as in (Parkhomchuk et al., 2009).  Libraries were prepared using Illumuna TruSeq adapters, and quantified by qPCR. Libraries were sequenced at NYU Genome Center with NextSeq 500 to produce pair-ended 75 bp reads.