title: GSM4802533: FAXINO80KO-1; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq with spike-in controls, 10 ul of 1:100 dilution of the ERCC Spike-In Mix #1 was added to the lysate of 1 million cells before RNA extraction. Total RNA was then extracted as described above. For RNA-seq library preparation, 1 mg total RNA was used to prepare the RNA-seq library with the Truseq RNA Library Prep Kit V2 with Ribo-Zero (Illumina). Truseq RNA Library Prep Kit V2 with Ribo-Zero (Illumina)