title: GSM5384670: SCC60A-Resistant-rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from sensitive and isiPI3K-acquired resistance UM-SCC47 and UT-SCC60A cell lines and sequenced. Three replicates of each of the sensitive and resistant cell lines were cultured in drug-free medium for 48 h, after which RNA was extracted using the RNeasy mini kit (74104, Qiagen, Hilden, Germany) according to the manufacturer's instructions. RNA-seq libraries were prepared using the TruSeq RNA Sample Preparation kit (Illumina, San Diego, CA, USA) according to the manufacturer's protocol. Briefly, 1ug of total RNA was fragmented, followed by reverse transcription and second-strand cDNA synthesis. The DS-cDNA was subjected to end repair, a base addition, adapter ligation, and PCR amplification to create libraries. Qubit and Bioanalyzer were evaluated. Sequencing libraries were constructed with barcodes to allow multiplexing of 6 samples in one lane