title: GSM4802387: FAXINO80KOH2azInput; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes at room temperature. Chromatin was fragmented by MNase digestion followed by ChIP. Immunoprecipitated DNA-protein complex was eluted with the elution buffer (50 mM Tris–Cl (pH 7.5), 10 mM EDTA, 1% sodium dodecyl sulphate) at 65°C for 15 min. The elution was collected and reverse-crosslinked at 65 °C overnight, diluted with 1 volume of TE buffer and treated with RNase A at 37 °C for 30 min followed by 0.2 μg/ml Proteinase K at 55 °C for 60 min. The immunoprecipitated DNA was purified from the elution by the Zymo DNA Clean & Concentrator Kit. ChIP-seq libraries were prepared as instructions of Nextflex Rapid DNA-seq Kit (PerkinEImer). Libraries of H2az1 CKO/H2az2 KO cell lines were prepared as instructions of Nextera DNA Flex prep kit.