title: Illumina HiSeq 4000 sequencing; DNA replication profiling by BrdU Immunoprecipitation of S.cerevisiae wild-type versus H3K37R mutant
library_construction_protocol: standard centrifugation YPAD medium at 30C G1 arrest (2h alfa factor at 30C)->YPAD+ 0.2M HU+0.5mg/ml BrdU (1h 15 minutes at 30C)-> 0.1% NaN3 (10 minutes in ice) Genomic Qiagen prep NEXTFLEX® ChIP-Seq Library Prep Kit for Illumina®Sequencing