title: GSM4802674: xrn1_B; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was isolated by a standard hot phenol method and contaminant DNA was removed by DNase I treatment (as in PMID: 23295673). Starting from total RNA, poly(A) RNA is reverse transcribed using a biotinylated adapter containing an anchored oligo-dT primer. After second strand synthesis, fragments are captured by streptavidin beads, end-repaired, adenylated, and ligated to a second adapter.  The library was prepared using  oligos that allows the sequencing into the poly(A) tail (from the body of the cDNA) (Internal protocol in  PMID: 23295673). Stringent library size selection (200bp was performed).