title: GSM6199158: sv 129 cells, with folate-deficiency-treated, input; Mus musculus; ChIP-Seq
library_construction_protocol: Total DNA was extracted by Simple ChIP Enzymatic chromatin IP kit (9005, CST) based on the manufacturer's protocol. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and IP samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 200-500 bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.