title: Illumina HiSeq 2000 paired end sequencing; Ethylene perception modulates tomato pollen tube growth by altering calcium signaling and cell wall remodeling
library_construction_protocol: pollen tube after 4 h of germination The germination medium contained 300 mM sucrose, 2 mM boric acid, 2 mM calcium nitrate, 2 mM magnesium sulfate, 1 mM potassium nitrate and 0,5 % (w/v) agarose, as described Twenty mg of the pollen powder were taken for total RNA extraction. The total RNA was isolated from the sample using the ReliaPrep™ RNA Tissue Miniprep System kit (Promega, Charbonnières-les-Bains, France) and treated with DNase I (Invitrogen, Illkirch, France) to remove DNA. cDNA