title: GSM5383040: Control 2; Homo sapiens; OTHER
library_construction_protocol: Total  gDNA was isolated from the buffycoat DNA and 250-500 ng was amplified through multiplex PCR of IGHV-IGHD-IGHJ gene rearrangements following the BIOMED-2 protocol. After amplification, PCR products were purified on Agencourt AMPURE beads (Beckman Coulter, Brea, CA, USA) and eluted using H2O. The purified product was checked for primer dimers using the Agilent Tapestation (Agilent,Santa Clara, CA, USA). The intitial multiplex PCR already attached the indexes to the PCR products, so library construction was simply pooling the purified PCR product in equilmolar fashion.