title: GSM5727266: P8_Testes_Srsf10 KO_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from whole testes or enriched cells using RNAzol® RT (Molecular Research Center. Inc, RN 190) following the manufacturer's instructions. The residual genomic DNA was removed with the DNase I Kit (Promega, M6101) For Bulk RNA-seq,  a total amount of 1 ug of RNA per sample was used to prepare cDNA libraries generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer's recommendations. For PacBio Isoform Sequencing (Iso-seq), 5 ug of total RNA per sample was used to prepare the Iso-Seq library using the Clontech SMARTer cDNA synthesis kit and the BluePippin Size Selection System protocol as described by Pacific Biosciences (PN 100-092-800-03). For Single-cell RNA-seq, Single cells were suspended in PBS containing 0.04% BSA and loaded onto the Chromium 3' v3 platform (10x Genomics) to generate single cell libraries according to the manufacturer's protocol.