title: GSM4802728: sgRUVBL1_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol following the manufacture's protocol RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's protocol. Briefly, polyA RNA was selected by RNA Purification Beads and fragmented enzymatically. The first strands of cDNAs were synthesized using Super Script II reverse transcriptase, and the second strands were synthesized using Master Mix. Double-stranded cDNA was then 3'-adenylated, ligated with index adaptors, and PCR-amplified. The final RNA-seq library was quantified using Bioanalyzer Agilent DNA 1000 Chips