title: RNA-SEQ THP-1 Cells
design: Total RNA was isolated from THP-1 cells. 200ng of RNA was used for RNA-seq library preparation using the Illumina Stranded mRNA prep. Purified mRNA was fragmented using oligo (dT) magnetic beads to capture messenger RNAs with polyA tails.  A pool of libraries ( 1.2pM)mix with Phix 2% were sequenced .