title: amplicon sequencing of Blastocystis SSU rDNA gene: horse feces
design: SQK-LSK110 Ligation Sequencing Kit and EXP-PBC001 PCR Barcoding Kit was used with MinION-adapted PCR primers for amplifying the SSU of the Eukaryotic rRNA gene directly from genomic DNA. "Ligation sequencing amplicons - PCR barcoding" protocol was referenced (version: PBAC12_9112_v110_revB_10Nov2020)