title: RNA-Seq; Toxoplasma gondii; PRP4_EtOH_R1
design: Parasite RNA was extracted using TRI Reagent (Sigma) followed by the RNeasy MinElute Cleanup Kit (Qiagen) according to the manufacturers protocol. DNase 1 (Qiagen) was used to remove contaminating DNA followed by a second cleanup. Library preparation and sequencing was performed by Victorian Clinical Genetics Services using TruSeq stranded mRNA kit (Illumina) and NovaSeq 6000 to a depth of 30M reads