title: Year-2_roots_ITS_rDNA_Salvia.sclarea_inoc_replicate = biological_replicate_5
design: 500 mg of ground root samples were used for total genomic DNA extraction using an adapted method, using Cetyltrimethylammonium bromide, Polyvinylpyrrolidone and activated charcoal extraction. The fungal ITS rDNA region  was then amplified in a PCR reaction using the  ITS3_KYO2_F/ITS4_KYO3_R primer pair, containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.