title: RNA-seq of Oculina arbuscula: Colony B aposymbiotic branch
design: RNA was extracted using the Invitrogen RNAqueous Total RNA Isolation Kit, with the addition of a DNase step to remove genomic DNA. Extracted RNA was normalized across samples using a NanoDrop spectrophometer. Library prep and sequencing was performed by NovoGene on two lanes of an Illumina Novaseq 6000 sequencer as 150bp paired-end reads. Sequencing depth ranged from 9.8 to 13.3 million reads per sample (11.5 million average), with average quality ranging from 40-42 across the full read length.