title: Bisulfite-Seq of Dorcoceras hygrometricum: leaves of A seedlings
design: The DNA library was constructed from a pool comprising equal quantities of DNA samples from six biological replicates. Genomic DNA was subjected to sonication, 3A addition, adaptor ligation, followed by bisulfite modified with a ZYMO EZ DNA methylation-Gold kit (Zymo Research, Orange, CA, USA), gel purification and PCR amplification. Sequencing was performed on an Illumina HiSeq X Ten platform (Illumina Inc., San Diego, CA, USA) with PE150 mode.