title: Bulk RNA-Seq of homo sapiens; adult; female left atrium; atrial fibrillation
design: Total RNA was isolated from all samples using the Direct-zol RNA Microprep Kit (Zymo Research), followed by a DNase digestion step. The integrity of the isolated RNA was assessed using the Bioanalyzer RNA 6000 Nano Kit (Agilent). After ribosomal (r)RNA depletion, a directional library preparation was carried out with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB). The quality of the resulting libraries was determined using the Bioanalyzer High Sensitivity DNA Kit (Agilent). Library quantification was performed using the NEBNext Library Quant Kit for Illumina (NEB). Next, equimolar library pools were sequenced in paired-end mode using 75 cycles on a NextSeq 500 system (Illumina) with v2 and v2.5 chemistry. Since the sequencing of human SR and AF samples was performed in separate batches, the SR samples were re-sequenced in lower concentration as spike-ins together with the AF samples. This allowed using the spike-in samples as technical replicates to address potential batch effects.