title: 16S amplicon sequencing of 12h fermented millet Kalariga
design: Amplification V4 region of 16S rRNA gene with specific primers 515F/806R (Caporaso, Lauber et al. 2011) and Phusion High-Fidelity PCR master mix (New England Biolabs, USA). Libraries using Illumina NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, England)