title: Year-1_roots_18S_rDNA_Salvia.sclarea_non-inoc_replicate = biological_replicate_4
design: 500 mg of ground root samples were used for total genomic DNA extraction using an adapted method, using Cetyltrimethylammonium bromide, Polyvinylpyrrolidone and activated charcoal extraction. The small subunit of 18S rDNA gene was then amplified using a Nested-PCR, with two successive PCR reactions using respectively the AML1_F/AML2_R and nu-SSU-0595-5_F/nu-SSU-0948-3_R primer pairs, containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.