title: RNASeq of Pinus pinaster grafted plants: Oria 6/ R1S
design: Frozen needles from each grafted pine were grinded using a MM400 Mixer Mill (Retsch GmbH and Co.) Total RNA was extracted from needle powder using Plant/Fungi Total RNA Purification Kit (Norgen Biotek Corp., Thorold. ON, Canada), following the manufacturers instructions. Integrity of the extracted RNA was evaluated by 1% (w/v) agarose gel analysis; concentration and quality were measured with a NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE). cDNA libraries were prepared using the Illumina kit TruSeq RNA library Prep. The 12 cDNA libraries were sequenced using an Illumina NextSeq 500 platform at ADM S.L. (Paterna, Valencia, Spain).