title: RNA sequencing of human: melanoma: Sample 148BR
design: 250-500ng of purified total RNA with DV200 scores >30% was considered acceptable for library preparation and sequencing.  First a stranded cDNA library from isolated RNA was constructed followed by hybridization of the library to a set of DNA oligonucleotide probes to enrich the library for mRNA transcript fragments (capturing 21,415 genes). The normalized, pooled libraries were loaded onto HiSeq2500 for a target of 50 million 2x76bp paired reads per sample.