title: ITS amplicon sequencing of Supernatant fermented millet Sunyani Sunyani
design: Fungal ITS amplification with primers ITS1F (5-CTTGGTCATTTAGAGGAAGTAA-3) (Jackson et al., 1999) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3) (White et al., 1990) and the KAPA2G Robust HotStart PCR Kit (Sigma). PCR products purified using KAPA Pure Beads (Roche). Library prepared with the KAPA2G Robust HotStart PCR Kit and the Nextera XT Index Kit v2 index primers (Illumina).