title: 16S rRNA-seq of freshwater lake microbial communities in Manasbal Lake
design: We performed PCR amplification of the V6-V8 region of microbial 16S rRNA genes. Pyrosequencing was subsequently performed at IBIS/Universit Laval Plate-forme dAnalyses Gnomiques (Qubec, Canada) using the Roche 454 GS-FLX Titanium chemistry