title: Transcriptomics of B. melanopygus embryo
design: RNA extraction was performed on a pooled embryo sample extracted from brood from a single black-form B. melanopygus colony. The embryos were homogenized for 35 seconds on low in the Trizol buffer in the Omni Bead ruptor, with 4 metal beads, followed by extraction using standard protocols in the Directzol RNA Miniprep Plus kit (Zymo Research). Sufficient RNA quality and quantity were confirmed using an Agilent Bioanalyzer, followed by preparation for Illumina sequencing using a TruSeq Stranded mRNA Kit (Illumina). The sample was sequenced 75-bp paired-end at the Pennsylvania State Genomics Core Facility (University Park, PA) using the Illumina NextSeq 550.