title: Haliclona oculata metagenome sequence reads
design: The DNAeasy PowerLyser Powersoil Kit was used to extract DNA from 0.2 g of the sponge specimen, with the final DNA elution in water and bead beating at 4000 rpm for 45 s. The Nextera DNA Library Prep Kit (Illumina) was used to prepare the sequence library with 25 ng of extracted DNA.