title: GBS of Lactuca sativa: Korean commercial lettuce varieties
design: Genotyping-by-sequencing (GBS) libraries were constructed for the 90 lettuce cultivars following the protocols described by Elshire et al. In brief, genomic DNA of each cultivar was digested with ApeKl (New England Biolabs, Ipswitch, MA) and ligated to a specific barcode adaptor. Digested DNAs were pooled and purified with the QIAquick PCR Purification Kit (Qiagen, Valencia, CA).