title: 16S rRNA sequencing of control marine sediments 6
design: Primers 341F (5 CCT AYG GGR BGC ASC AG 3) and 806R (5 GGA CTA CNN GGG TAT CTA AT 3) were used to amplify the V3-V4 region of the 16S rRNA gene. All PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix (New England Biolabs). PCR products were mixed at equal density ratios. The pool was then purified with Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated with NEBNext UltraTM DNA Library Prep Kit for Illumina and quantified via Qubit and q-PCR. Finally, the NovaSeq 6000 Sequencing System (2 x 250 bp) was employed for sequencing the samples.