title: Cecal shotgun metagenomics of AGP treated broilers
design: DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (ZymoBIOMICS, Irvine, CA) according to the manufacturers protocol. The isolated DNA was quantified by Qbit (ThermoFisher, Coon Rapids, MN). DNA libraries were prepared using the Illumina Nextera XT library preparation kit (Illumina, San Diego, CA), with a modified protocol.