title: 85srRNA of ocular surfaceadult eye
design: The V3V4 region of the 16S rRNA gene was amplified from extracted genomic DNA samples with primers (319 F: 5-ACTCCTACGGGAGGCAGCAG-3 and 806 R: 5-GGACTACHVGGGTWTCTAAT-3). The PCR amplification products were purified with Agencourt AMPure XP magnetic beads, dissolved in elution buffer, and then labeled. The fragment range and the concentration of the library were detected using the Agilent 2169 Bioanalyzer