title: WGRS of A. m. jemenitica: Saudi Arabia:A43.
design: Images generated by sequencers are converted by base calling into nucleotidesequences, which are called raw data or raw reads and are stored in FASTQ format.FASTQ files are text files that store both reads sequences and their correspondingquality scores. Each read is described in four lines as follows:@FCB068CABXX:6:1101:1403:2159#TAGGTTAT/1GTAGAAGACTTATAGATTAAAATTCTCCAACATATAGATGTCCTTACACCGTTTTCCTTTGCTCAGCAGGCTCCGTGTTTGCTTGTCCTT+c`bcc_c^ccde_df\c_aeff`ffcfffdfedadca^`b_eed`fe\fed\babdba^Yeebeccfdeae_eec^dbXbda`]bcbebcThe line 1 and 3 are sequence name generated by the sequencer; line 2 is sequence; line4 is sequencing quality scores, in which every letter corresponds to a base in line 2; thebase's sequencing quality is the ASCII value that the letter in line 4 refers to minus 64(Specification). For example, the ASCII value of c is 99, so the correspondingsequencing quality value is 35. Solexa quality value of sequencing bases ranged from2 to 35.