title: RNA-Seq WB2.tet A. aegypti 2 days post emergence
design: Small RNAs were extracted from mosquitoes using  miRNeasy Mini Kit (Qiagen). Genomic DNA was removed using Turbo DNA-free kit  (Invitrogen) as per the manufacturers instructions. Total RNA of each sample  was quantified and qualified by Agilent 2100 Bioanalyzer (Agilent  Technologies, Palo Alto, CA, USA), NanoDrop (Thermo Fisher Scientific Inc.)  and 1% agrose gel.