title: RNA-Seq of Humo sapiens: 8 year-old B-ALL male
design: Total RNA quantification, preparation of libraries from 100ng of total RNA, quality assessment and sequencing of libraries. Illumina NovaSeq base call (BCL) files were converted in fastq file through bcl2fastq. Sequence reads were trimmed using bbduk software. Differential expression analyses were performed using edgeR on genes having more than 1 CPM in more than the minimum number of samples belonging to one condition minus 1 and less than 20% of multi-mapping reads, simultaneously and genes having FDR below 0.05 were selected as differential expressed.