title: TCR of human esophageal squamous cell carcinoma (beta chain)
design: gDNA of each sample were extracted, and subjected to amplify TCR alpha and beta repertoire using multiple PCR. TheV gene segments, J gene segments, and the whole complementarity determiningregion 3 (CDR3) were captured by PCR reactions. Amplification products weresequenced by xTen with 150-bp paired-end reads. Raw sequencing data wereprocessed and filtered using IMonitor. Clean sequences were aligned to V and Jgermline alleles (IMGT database, www.imgt.org), and V and J gene segments wereassigned for each clone. Meanwhile, the sequencing errors were corrected in termsof sequencing qualities and low-frequent sequences (101 in one million) were filteredout.