title: Year-1_soil_18S_rDNA_Salvia.sclarea_non-inoc_replicate = biological_replicate_5
design: 250 mg of rhizospheric soil samples were used for total genomic DNA extraction using the Nucleospin@ soil kit (Macherey-Nagel). The small subunit of 18S rDNA gene was then amplified using a Nested-PCR, with two successive PCR reactions using respectively the AML1_F/AML2_R and nu-SSU-0595-5_F/nu-SSU-0948-3_R primer pairs, containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.