title: 16S rRNA-based fecal bacterial composition of neonatal dairy calves - Fecal microbiota transplantation
design: Fecal sample collected from neonatal dairy calves subjected to a fecal microbial transplantation (1x/day) from 8 to 12 days of age (Sample #21). Sample kept at -80C until microbial genomic DNA extractions. Microbial genomic DNA was extracted using repeated beating followed by Qiagen stool kit. PCR amplification of bacterial V1-V3 regions of 16S rRNA genes. Sequencing using Illumina MiSeq2X300.