title: RNAseq of Cryptobranchus alleganiensis skin: Topical Vaccinated on experimental day 89
design: Total RNA was extracted from RNAlater-stored sample with TRIzol. Total RNA was Dnase treated with TURBO-DNA free, rRNA depleted with Ribo-Zero Epidemiology following the Clontech protocol, and libraries were prepared using Illumina ScriptSeq v2 kits. Libraries were pooled, treated with Illumina free adapter blocking reagent, and sequenced on an Illumina NovaSeq 6000.