title: Modelling Multi-Dimensional ClinOmics for Precision Therapy of Children and Adolescent Young Adults with Relapsed and Refractory Cancer: Sample EWS5202tumor
design: Total RNA was extracted using Qiagen AllPrep DNA/RNA mini kits. Ribozero stranded RNA libraries were prepared for RNA sequencing on Illumina HiSeq2000, 2500, and NextSeq500 according to the manufactures protocol (Illumina, San Diego, CA). Sequencer-generated bcl files were converted to fastq files using the bcl2fastq tool in CASAVA (Illumina, San Diego, CA) suite.