title: Shotgun seq-Phone-HBA-1
design: Libraries were prepared according to the manufacturers protocol using Nextera DNA Flex Library Preparation Kit (Illumina San Diego, CA USA). Preparation and bead clean-up were run on the Mantis Liquid Handler (Formulatrix) and Epmotion (Eppendorf) automated platform. On completion of the library prep protocol, each library was quantified, and quality control (QC) was performed using the Quant-iT dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA USA) and Agilent D1000 HS tapes on the TapeStation 4200 (Agilent Technologies, Santa Clara, CA USA) as per manufacturers protocol. Library Pooling, QC and Loading Nextera DNA Flex libraries were pooled at equimolar amounts of 2nM per library to create a sequencing pool. The library pool was quantified in triplicates using the Qubit dsDNA HS Assay Kit (Invitrogen). Sequencing was carried out on the NextSeq500 (Illumina) using NextSeq 500/550 High Output v2 2 x 150bp paired end chemistry according to manufacturers protocol. 12   The post-sequencing derived raw data were retained and transferred into Illumina base space platform (https://basespace.illumina.com).