title: Acquired Resistance Mechanisms to Osimertinib in EGFR Mutant Lung Cancer: Sample Sample_CL0038_T3R_T_HGGLYBGX2
design: RNA-Total RNA was extracted using Qiagen AllPrep DNA/RNA mini kits. RNA libraries were prepared using TruSeq stranded mRNA library kits (Illumina) and sequenced on Illumina HiSeq2000, 2500, and NextSeq500 according to the manufactures protocol (Illumina, San Diego, CA). Sequencer-generated bcl files were converted to fastq files using the bcl2fastq tool in CASAVA (Illumina, San Diego, CA) suite.