title: Metagenomic analysis of the bacterial soil communities of BC+ soils by amplifying the V3-V4 regions of 16S rRNA gene
design: Illumina technology sequencing (MiSeq run PE 2x250) of the V3-V4 regions of the 16S rRNA gene using Illumina 16S primers 341F and 805R. DNA template was extracted from the rhizospheric soil by the bead-beating method, following the manufacturers instructions for the MoBio UltraClean Soil DNA Isolation kit (MoBio laboratories, Solana Beach, CA, USA).