title: scATAC-seq of peripheral blood mononuclear cells from a patient with MELAS: Sample P21
design: "Peripherial blood mononuclear cells were recovered from a patient with MELAS. We stained cells for viability and applied antihuman CD45 antibodies before fixation and performed fluorescence-activated cell sorting (FACS) to exclude dead and nonleukocyte cells (CD45-). The mtDNA single-cell ATAC sequencing libraries were generated by a 10x Chromium Controller and a modified Chromium Single Cell ATAC Library and Gel Bead Kit protocol, which was followed by paired-end sequencing."