title: NGN2_ATAC_seq_line_08
design: ATAC-seq samples were prepared as previously described 13,54. Briefly, 75,000 viable cells were lysed in ATAC-Resuspension Buffer (RSB) containing 0.1% NP-40, 0.1% Tween-20, and 0.01% Digitonin for 3 min on ice. Nuclei were washed and resuspended in transposition mixture. Reactions were incubated at 37C for 30 min on a thermomixer at 1,000 rpm. Transposed DNA was purified by DNA Clean and Concentrator-5 Kit (Zymo). Eluted DNA was shipped to the University of Minnesota Genomic Center for library preparation and ATAC-seq. All raw sequence reads generated by Illumina HiSeq 2000 had been demultiplexed at the University of Minnesota Genomics Center and provided as 275 bp paired-end fastq files (targeting 60 M reads per sample).