title: DL-sncRNA-MO-17.5-4
design: NEBnext multiplex Small RNA Library Preparation Set 1 for Illumina sequencing (New England Biolabs, Ipswich, MA, USA). Size fraction was carried out as recommended by the manufacturer by running a (6%) polyacrylamide gel (Lonza, Basel, Switzerland) at 4 ?C for 1 h. Each sample was tagged with different multiplex identifier tags provided by NEB. The generated miRNA libraries were evaluated by DNA high sensitivity Chip (BioAnalyzer, Agilent) prior to sequencing. Quantification of the libraries was carried out with Qubit (Life Technologies, Carlsbad, CA, USA) and 4 nM was single strand sequenced on the Illumina NextSeq sequencing platform at the Microchemistry laboratory of Forth, Crete, Greece.