title: RNA-Seq WB2 A.  aegypti 2 days post emergence
design: Small RNAs were  extracted from mosquitoes using miRNeasy Mini Kit (Qiagen). Genomic DNA was  removed using Turbo DNA-free kit (Invitrogen) as per the manufacturers  instructions. Total RNA of each sample was quantified and qualified by  Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), NanoDrop  (Thermo Fisher Scientific Inc.) and 1% agrose gel.