title: RNA seq of goose pectoral muscle
design: The RNA libraries were constructed and deep sequencing was performed by Biomarker Technologies (BMK, commercial service). We extracted total RNA from frozen pectoral muscle utilizing TRIzol reagent (Invitrogen) according to the manufacturers protocol. We then evaluated RNA integrity and concentration by Agilent 2100 Bioanalyzer (Agilent Technologies). For each stage and breed, equal quantities of RNA isolated from three individual muscle tissues were used. Illumina HiSeqTM 2000 were used for sequence tag preparing. Clean tags were obtained with the criteria of removing adaptor tags, low quality tags and tags of copy number = 1. The clean tags were classified according to their copy numbers in the library. The proportion of each category in relation to total clean tags was determined. Similar analysis was carried out for clean distinct tags. The saturation analysis of the sequencing library was completed by BMK. For reads mapping, sequences were obtained from Anser cygnoides domesticus (assembly AnsCyg_PRJNA183603_v1.0).