title: Haliclona xena metagenome sequence reads
design: For DNA extraction bead beating was performed with the DNAeasy PowerLyser Powersoil Kit at 4000 rpm for 45 s, followed by an overnight incubation at 56 C  with 0.2 M EDTA, 10 % CTAB, 0.5 M NaCl,  0.1 M EDTA (1:4 ratio), _-Mercaptoethanol, and Proteinase  K. RNAse A (0.3 ng/_l) was then added and the sample incubated for 30 min at 37 C. Chloroform/Isoamylalcohol (24:1) addition, incubation (2 min), and washing (centrifugation at 10000 x g for 10 min) was performed twice. DNA was precipitated in isopropanol for 6 hours, pelleted, and washed twice in ethanol before DNA elution in water. The Nextera DNA Library Prep Kit (Illumina) was used to prepare the sequence library with 25 ng of extracted DNA.