title: TCR of human: gastrointestinal cancers
design: Total RNA was reverse transcribed using SuperScript IV VILO Master Mix. For each sample, cDNA was amplified using the Oncomine TCR Beta-LR Assay. Libraries were purified with Agencourt AMPure XP beads, washed with 70% ethanol, and eluted in Low TE buffer. Resulting library samples were diluted and quantified using the Ion Library Quantitation Kit, then diluted to 25 pM with Low TE buffer.