title: MADS27_ox_N_up_rep3
design: RNA from roots was extracted as described above and analyzed with RNA 6000 Nano Chips and Agilent Bioanalyzer System (Agilent Technologies, USA, No.5067-1511). Samples with RIN numbers above 8 were further submitted to rRNA depletion with a use of RiboMinus Plant Kit for RNA-seq (Invitrogen, USA, No.2157461) and RNA concentration was further assessed with Qubit 3.0 Fluorometer (Invitrogen, USA). 100 ng RNA per library was used. NEB Ultra II Directional RNA Library Prep kit for Illumina (New England BioLabs, USA, No. E7760S) was used according to the manufacturers protocol and the quality of prepared libraries was assessed with Agilent Bioanalyzer DNA Chip (Agilent Technologies, No. 6067-1504, USA).