title: wild type_70DAS_rep1
design: The RNA-Seq paired end libraries were prepared using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA). Starting with total RNA, mRNA was purified using poly (A) selection, then the RNA was chemically frag-mented and converted into single-stranded cDNA using random hexamer primers. Next, the second strand was generated to create double-stranded cDNA. Library con-struction began with generation of blunt-end cDNA fragments from ds-cDNA. Then, A-bases were added to the blunt ends to prepare them for ligation of sequencing adapters. After size selection, the ligated cDNA fragments containing adapter se-quences were amplified by PCR using adapter-specific primers. The library was quan-tified with the KAPA library quantification kit (#KK4854, Kapa Biosystems, Woburn, MA, USA) following the manufacturer's instructions. Each library was subjected to high-throughput sequencing on the Illumina Hiseq2000 platform.