title: Anthocerobacter long DNA Reads
design: Genomic DNA was sequenced on both Oxford Nanopore MinION and Illumina platforms. Nanopore libraries were prepared using the Ligation Sequencing kit (SQK-LSK109) and sequenced on two MinION R9 flowcells (FLO-MIN106D) for 60 hours. In one MinION run, the focal sample was multiplexed with another cyanobacteria sample and barcoded by Native Barcoding Expansion Kit (1-12), while the other run contains only the focal sample. Basecalling was carried out using Guppy v3.1.5 with the high accuracy flip-flop mode. The Illumina  library was prepared and sequenced on NextSeq500 by MiGS (Microbial Genome Sequencing Center).