title: RNA-Seq of mus musculus: adult male liver
design: Mouse liver tissue samples were lysed using QIAzol Lysis Reagent and the TissueLyser, the aqueous phase was recovered, ethanol was added and processed following the RNeasy 96 Universal Tissue protocol. An on-column DNase treatment step was included. RNA was eluted in nuclease-free water. Total RNA samples were prepared according the TruSeq Stranded mRNA Sample Preparation Guide (Rev. E). The mRNA fragments were converted to double-stranded cDNA (ds cDNA) using SuperScript II and purified with AMPure XP beads. The adapter-ligated DNA products were purified with AMPure XP beads. Adapter ligated DNA was amplified in a Linker Mediated PCR reaction. Paired-end 2x150bp sequencing was performed, using standard SBS chemistry on an Illumina NovaSeq6000 sequencer.