title: RNA-seq of mus musculus: thymic medullary fibroblasts, IKKA(Y580C/Y580C), replicate 1
design: mRNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen), followed by cDNA synthesis using the SuperScript VILO cDNA Synthesis Kit (ThermoFisher Scientific). The Ion AmpliSeq Transcriptome Human Gene Expression Kit was used to prepare barcoded libraries and sequenced using an Ion S5TM next-generation sequencer. The AmpliSeqRNA plugin (ThermoFisher) was used to calculate differential gene expression analysis.