title: illuminaSeq: plasmide B1839
design: For DNA extraction bacteria were centrifuged at 3000g for 10 min. The bacterial pellet was suspended in a 1 ml of lysis buffer containing 20  Tris-HCL (pH 8.0), 2  EDTA, 1.2% Triton X-100, and 20 mg/ml lysozyme, and incubated for 30 min at 37C. Genomic DNA of the strain was extracted using a GeneJET Genomic DNA Purification Kit #K0721 (Thermo Fisher Scientific, Waltham, Massachusetts, USA) according to the manufacturers instructions. The DNA quality was evaluated using 1% agarose gel electrophoresis, an UV5Nano spectrophotometer (Mettler Toledo, USA), and Qubit 2.0 Fluorometer (Thermo Scientific, Waltham, MA, USA). The optical density ratio 260/280 nm and 260/230 nm was >1.8 and >2.0, respectively. DNA was stored at -20C until further processing.