title: Nanopore fail reads
design: A total amount of 2 g DNA per sample was used as input material for the ONT library preparation. After the sample was quantified, size-select of long DNA fragments was performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction was conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of library fragments.