title: BCR-Seq of Orictolagus cuniculus: Blood, spleen and bone marrow samples
design: Total RNA was isolated from spleen, bone marrow and peripheral blood mononuclear cell (PBMC) using TRIzol Reagent (15596018; Invitrogen). Then, cDNA was synthesized using 1 g of total RNA and SuperScript  first-strand cDNA synthesis kit with oligo dT priming (18091050; Invitrogen). From the cDNA, VH and VK amplicons were prepared in singleplex and multiplex PCR.