title: RNA-seq analysis of winter rye grains (cultivar Tatarskaya-1): dough (wax) stage
design: Rye kernels from cultivar Marusenka from the middle part of the spikes (1015 homogeneous spikes were used for each biological replicate)were fixed with liquid nitrogen and stored at -80C until RNA extraction. The collected samples were ground in a homogenizer (MM400). The total RNA was isolated with ExtractRNA reagent (Evrogen) combined with the RNeasy Mini Kit (Qiagen) and Plant RNA Isolation Aid (Ambion). The llibrary construction and sequencing were performed by the Novogene Corporation (China; https://en.novogene.com/). Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads with the BBDuk utility [BBTools, https://jgi.doe.gov/data-and-tools/bbtools/].