title: RNA-Seq of Dorcoceras hygrometricum: leaves of F4 seedlings
design: Total RNA was extracted using a Plant Total RNA Extraction kit (Sigma, St. Louis, MO, USA) and purified with DNase I (Promega, Madison, WI, USA). The cDNA synthesized from enriched poly(A) mRNA was connected with adaptors, PCR-amplified and then sequenced using an Illumina Hi-Seq 4000 platform (Illumina Inc., San Diego, CA, USA), with 150-bp paired-end reads (PE150).