title: RNA-Seq of Cryptotermes secundus, queen, Protein+ colony (colony ID FD45 T2)
design: Examination of gene expression patterns in Cryptotermes secundus queen (Protein+ colony, head tissue). Tissue was preserved in RNAlater (Qiagen) and kept at 4C for 24 h, then at -20C for 24h and at -80C until further processing. Total RNA was isolated from thawed tissue samples with the peqGOLD Tri Fast Kit using chloroform, nuclease-free glycogen (5 mg/ml), Ambion isopropanol, and 75% ethanol. DNA was digested by DNase I Amplification Grade (Sigma Aldrich, Cat. No. AMPD1). cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina) and the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific).