title: metagenomic sequencing library of estuarine sediment from Jiulong River
design: Note: please contact Dr. Kao (sjkao@xmu.edu.cn) before the using this dataset.  The surface sediment was collected in December 2018 during a cruise on the Jiulong River estuary using a grabber and immediately sealed in 50-ml tubes (Falcon). The sediments were quickly frozen in liquid nitrogen and stored at 80C until DNA extraction was performed. DNA was extracted using a PowerMax soil kit (Qiagen), following the manufacturers instructions. Shotgun sequencing was performed using the Illumina (San Diego, CA, USA) HiSeq 2000 paired-end 150-bp platform with a HiSeq cluster kit v4.