title: Year-1_soil_ITS_rDNA_Salvia.sclarea_non-inoc_replicate = biological_replicate_2
design: 250 mg of rhizospheric soil samples were used for total genomic DNA extraction using the Nucleospin@ soil kit (Macherey-Nagel). The fungal ITS rDNA region was then amplified in a PCR reaction using the ITS3_KYO2_F/ITS4_KYO3_R primer pair, containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.