title: rbm46_2
design: Testes mRNA profiles of 30dpf post-fertilized wild-type (WT) and RNA binding protein 46 knockout (Rbm46/) zebrafish were generated by deep sequencing, mixed sample sequencing using Illumina NextSeq500. The reference genome index was firstly established through Bowtie2, then paired-end clean reads were mapped to the zebrafish reference genome using Tophat2 v2.1.0 software according to the standard protocols. Differential expression analysis of control and rbm49 mutant samples was performed using CuffDiff . qRTPCR validation was performed using TaqMan and SYBR Green assays.