title: WES of human esophageal squamous cell carcinoma
design: Each tumor was divided to five regions and a total amount of 0.6 g genomic DNA per sample was used as input material for the DNA sample preparation. Sequencing libraries were generated using Agilent SureSelect Human All Exon V6 kit. Captured libraries were enriched by PCR to add index tags to prepare for sequencing. Products were purified using the AMPure XP system (Beckman Coulter, Beverly,USA) and quantified using the Agilent high sensitivity DNA assay on the AgilentBioanalyzer 2120 system.