title: Iso-seq transcripts from 5 tissues (7-d old leaf, 7-d old root tips, 6-d old hypoxic coleoptile, growth zones from 118-d old plants, developing grain [including husk, rachillae, and pollen] from 218-d old plants) of O. australiensis from Keep River region near Kununurra (Western Australia).
design: Total RNA was extracted from 5 tissues of O. australiensis plants (7-d old leaf, 7-d old root tips, 6-d old hypoxic coleoptile, growth zones from 118-d old plants, developing grain [including husk, rachillae, and pollen] from 218-d old plants) using the Sigma Spectrum Plant Total RNA kit as per manufacturers instructions (except alpha-amylase was added to the developing grain extract to reduce the inhibitory effects of excess polysaccharides on RNA extraction). RNA was aliquotted into RNAStable tubes and sent to DNA Link in South Korea for library preparation and sequencing for an IsoSeq3 workflow, which was managed using SMRT Link 8.0.0 software. The kits used for library preparation were the SMATer PCR cDNA Synthesis kit & the DNA Template prep kit 1.0. Part of the library preparation was multiplexing the different RNA samples. After sequencing, reads were demultiplexed based on barcodes sequences, and Arrow was used with a 99% accuracy setting to define High and Low quality consensus sequences for each detected isoform. Reported here are the high quality consensus sequences.