title: RNA-Seq Aa23-R1
design: Aa23 and Aag2.wAlbB cells were grown in 25cm2 flasks until  confluent. Total RNA was extracted from three pellets from each flask using  RNeasy Mini Kit (QIAGEN), treated with DNase I (Turbo DNase, Ambion),  quantified using an Epoch spectrophotometer (BioTek), and checked for  integrity using gel electrophoresis. RNA sequencing was performed by GENEWIZ