title: NGN2_RNA_seq_line_07
design: Total RNA was isolated using RNeasy plus kit (QIAGEN) following the vendors instructions and quantified by a NanoDrop-8000 spectrometer. The isolated RNA was subsequently sent to the sequencing provider (GENEWIZ) for further processing and sequencing analysis. RNA-seq files were provided in the format of 2150 bp paired-end fastq files. Briefly, libraries were prepared using the NEB Nextera kit with customized adapters. Since the sequencing facility had performed pre-cleaning on raw reads, no Trimmomatic adapter-trimming was performed. 20~30 M reads were recovered from each sample.