title: Oxford Nanopore WGS of Vagococcus fluvialis 25B2
design: Long-read sequencing libraries were constructed using the Ligation Sequencing Kit SQK-LSK109 and the Native Barcoding Expansion 1-12 EXP-NBD104 (Oxford Nanopore Technologies, Oxford, United Kingdom) according to the manufacturers instructions. The library was subsequently loaded onto a primed R9.4.1 SpotON Flow Cell on a MinION device (Oxford Nanopore Technologies, Oxford, United Kingdom). Sequencing was run for 36h using the MinKNOW software 19.06.8 for data acquisition and Guppy v4 for basecalling. Adapter trimming and barcode demultiplexing (75% barcode sequence identity threshold) of generated reads was performed using Porechop v0.2.4 (https://github.com/rrwick/Porechop).