title: Shotgun metagenomic sequencing of gut homogenate from swine with L. intracellularis infection
design: DNA and RNA extraction was performed using MagMax Core Nucleic Acid Purification Kit (ThermoFisher). cDNA was synthesized from RNA with the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Thermo Fisher). Library preparation was performed with the Nextera XT kit (Illumina) and sequencing performed on MiSeq sequencer with 2x300 bp V3 chemistry (Illumina).