title: 16S rRNA gene samples collected in treatment sample 4 in February
design: Microbial genomic DNA was extracted from the gut contents samples using the PowerFecal DNA Isolation Kit (Mo Bio, CA, USA) following the manufacturers instructions. The V4-V5 hypervariable region of the bacterial 16S rRNA gene was amplied with a forward primer 515F and a reverse primer 909R containing a unique 15-base barcode.