title: RNA-Seq of Neocalanus Flemingeri: 2016 Adult Female Week 5 S35
design: Total RNA was extracted from individual females using QIAGEN RNeasy Plus Mini Kit (catalog # 74134) in combination with a Qiashredder column (catalog # 79654) and shipped on dry ice to the Georgia Genomics Bioinformatics Core (https://dna.uga.edu). Double-stranded cDNA libraries (NGS Stranded) were multiplexed and sequenced using Illumina Next-Seq 500 instrument (High Output Flow Cell, 75 bp, paired end).