title: RNA-Seq of Arabidopsis plants over expressing AtAHL20
design: Total RNA was extracted from rosette leaves harvested from 20-day old growth-chamber-grown plants using MagJET Plant RNA Purification Kit (Thermo Scientific). The Dynabeads mRNA DIRECT Kit (Thermo Scientific) was used for purification of intact polyadenylated (polyA) mRNA. RNA-Seq libraries used were prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies) following the manufacturers protocol.