design: The elite hybrid  variety Jingke968 performs better seed germination ability compared with its  parents, Jing724 and Jing92. To investigate the molecular basis of seed  germination ability heterosis of Jingke968, we carried out the RNA-seq assays  with the germinated embryo RNAs of Jing968, Jing724 and Jing92 under low  temperature (10 degrees Celsius) and room temperature (25 degrees Celsius as  control) treatments. The genes involved in seed germination ability heterosis  will be identified.