design: The following kits were used according to the manufacturer's instructions: RNAm TruSeq Stranded LT-Set A kit (RS-122-2101) (Illumina, San Diego, CA, EUA): for messenger RNA purification and cDNA library construction; HiSeq Rapid PE Cluster Kit v2 (PE-402-4002); SBS Kit v2 (200 Cycle; FC-402-4021); and TruSeq Stranded mRNA LT - Set A (RS-122-2101): to obtain paired-end reads, with 100 pb in length.