design: DNA was extracted using the MoBio Powersoil DNA Isolation kit (MO BIO, Carlsbad, CA, USA). A clean filter was used as negative control for the extractions. DNA extracts were first used for 16S rRNA gene amplification (30 cycles) and sequencing on an Ion Personal Genome Machine (Ion Torrent, Life Technologies, Paisley, UK), using primers F515 and R926 (Quince et al., 2011) and procedures as described previously (de Rezende et al., 2020)