design: PCR were done in technical duplicate, using high fidelity Q5 polymerase, and primers BITS (5 ACCTGCGGARGGATCA 3) and B58S3 (5 GAGATCCRTTGYTRAAAGTT 3), targeting a portion of ITS fungal region. Amplicons were purified, and ligated with Illumina adaptators, prior to MiSeq sequencing using 2x150 cycles.