design: Samples were prepared according to the TruSeq Stranded mRNA protocol (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/samplepreps_truseq/truseq-stranded-mrna-workflow/truseq-stranded-mrna-workflow-reference-1000000040498-00.pdf). Library prepraration involved adapter ligation, fragmentation, end repair and Poly(A) capture.