design: Genomic DNA was fragmented to 12 kb fragments with Covaris g-tubes. The library was prepared using Pacific Biosciences protocol for preparing multiplexed microbial SMRTbell libraries; barcoded hairpin adapters and PacBio Barcoded Adapter. The library was sequenced using Sequel Polymerase v3.0, SMRT cells v3 LR and Sequencing chemistry v3.0.