design: Genomic DNA was isolated and purified from abdominal muscular tissue using a Tissue Ruptor (Qiagen), followed by proteinase K digestion and isopropanol precipitation. The quality of isolated genomic DNA was assessed via agarose gel electrophoresis and/or via 2200 TapeStation (Agilent). PCR genotyping was performed as described before: Gutekunst, 2018.