design: CTAB genomic DNA extraction was performed on leaf tissue, and quantified by Nanodrop and Qubit 2.0. NEBNext Ultra II DNA Library Prep Kit for Illumina, clustering, and sequencing reagents were used per manufacturer's recommendations. Libraries were validated with DNA 1000 Chip on Agilent 2100 Bioanalyzer and quantified by Qubit 2.0 and RT-PCR.