design: Sequencing librairies construction with the Universal Plus mRNA-Seq Library Preparation with NuQuant kit from Nugen Tecan Genomics, from 1 g of total RNA: poly(A) RNA Selection, RNA Fragmentation, First Strand Synthesis, Second Strand Synthesis, End Repair, Adaptor Ligation, Strand Selection, Lybrary Amplification (8 PCR cycles)