design: 50 mg of V. amurensis tissue was taken 800 microlitres buffer with an 2*content of salt (100 mM Tris pH 7.5, 1.4 M NaCl, 40 mM EDTA pH 7.5, 1% CTAB) was added, incubated for 1 hour 60C in a water bath WB-4MS (BioSan, Latvia). Then 300 microlitres of chloroform was added, gently mixed and centrifuged for 10 min 13,200 rpm (Ependorf, USA). Further, a supernatant was selected in separate test tubes with 950 microlitres of 96% ethanol and frozen at -20C overnight. Next, the DNA in ethanol was centrifuged 10 min 13,200 rpm, after which the supernatant was removed and the precipitate was dried until the ethanol completely evaporated at room temperature. The precipitate was dissolved in 100 microlitres of distilled water and then purified on Plasmid DNA purification columns Plasmid MiniPrep kit per manufacturers protocol (Evrogen, Moscow, Russia). As a result, the DNA was dissolved in 50 microlitres of water. DNA was assessed for quality and quantity using the NanoPhotometer P300 (IMPLEN, USA). DNA samples were sent to Evrogene (Moscow, Russia) for high-throughput sequencing using Illumina technology. Libraries were prepared for sequencing according to the protocol described in the manual "16S Meta-genomic Sequencing Library Preparation" (Part # 15044223 Rev. B; Illumina). Bacterial 16S rRNA regions were amplified from all samples using the modified for Vitis sp. plants primers 515F (GGTAATACGKAGGKKGCDAGC) and 806R (RTGGACTACCAGGGTATCTAA). After obtaining the amplicons, the libraries were purified and mixed equimolarly using the SequalPrep Normalization Plate Kit (ThermoFisher, Cat # A10510-01). Quality control of the obtained library pools was performed using Fragment Analyzer and quantitative analysis was performed using qPCR.The library pool was sequenced on Illumina MiSeq (2 x 250 paired end) using MiSeq Reagent Kit v2 (500 cycles). The FASTQ files were obtained using bcl2fastq v2.17.1.14 Conversion Software (Illumina). The phage PhiX library was used to control sequencing parameters. Most of the reads pertaining to phage DNA were removed during demultiplexing.