design: We extracted genomic DNA from Cas9 genome-edited HAP1-derived CML cells. We then used PCR to amplify the loci of interest (either EMX1 or FANCF) and loci that constitute possible off-targets for the genome editing. The forward primers for the PCRs contained barcodes to distinguish the PCR products in each pool. The PCRs were then pooled and PCR-purified. Finally the samples were sent to Baseclear B.V. for sequencing on their NovaSeq6000.