design: viral nucleic acid was extracted using QIAamp viral RNA mini kit (QIAGEN, Germany). First strand cDNA synthesis was done using Promega GoScript Reverse transcriptase system (Promega corporation, USA).  Enrichment was done using COVIDSeq Primer Pool 1 & 2 (CPP1 & CPP2). Library was prepared using Illumina COVIDSeq Assay kit according to guideline (document # 1000000126053 v07). Prepared libraries were purified and pooled up and then quantified with Qubit ds DNA HS assay kit. Libraries were normalized to 4 nM concentration. Pooled normalized libraries were denatured and finally diluted to 1.2 nM concentration according to Illumina Miniseq system denature and dilute libraries guide (document # 1000000002697 v04).