design: Genomic DNA samples were normalized to 0.2ng/uL in preparation for whole genome shotgun library preparation using the Illumina Nextera XT library Preparation kit following the manufacturers protocol. Libraries were validated for length (450bp average library length; average insert size of 315bp) and adapter dimer removal using the Agilent TapeStation 4200 D5000 High Sensitivity assay then quantified and normalized using the dsDNA High Sensitivity Assay for Qubit 3.0. Sample libraries were prepared for Illumina sequencing by denaturing and diluting the libraries per manufacturers protocol. All samples were combined into one sequencing pool, proportioned according to expected number of reads, and run as one sample pool on the Illumina NovaSeq 6000 SP 300 cycle sequencing kit, version 1.5 chemistry.  Target read depth was achieved per sample with paired end 150bp reads.