design: CD4+ T cells were isolated using the EasySep CD4+ T cell isolation kit (STEMCELL, 17952). DNA was extracted from cultured CD4+ T cells using the Qiamp micro-DNA kit according to the manufacturers instructions. TCRseq of DNA extracted from cultured CD4+ T cells was performed by the Johns Hopkins FEST and TCR Immunogenomics Core Facility (FTIC) using the Oncomine TCR Beta short-read assay (Illumina, Inc). Samples were pooled and sequenced on an Illumina iSeq 100 using unique dual indexes.