design:  Whole genome sequence consists of 2 x 150 bp paired end reads with a minimum of 30 x average coverage or 90 Gb of unique, aligned reads from Illumina NovaSeq 6000 instruments running the Illumina Xp workflow.  PCR-free sequencing libraries start with 500 ng input DNA, sheared with Covaris and doubly size selected using different ratios of AMPure XP beads to select a narrow range of fragment lengths.  Each library uses Kapa Hyper PCR-free library reagents (KK8505, Kapa Biosystems) and Illumina TRUseq UD dual index adapters (cat # 20022370).  Libraries are multiplexed before sequencing. 