design: Total DNA was isolated from 20 pooled adult abdomina (without wings) using QIAGEN Genomic-tip 20/G. Long-read sequencing was performed on a MinION Mk1B Sequencing System. Upon receipt of flowcells, and again immediately prior to sequencing, the number of pores on flowcells was measured using the MinKNOW software. Flowcells were replaced into their packaging, sealed with parafilm and tape, and stored at 4C until use. Library preparation was performed with the Ligation Sequencing Kit (SQK-LSK109) and completed libraries were loaded on a flowcell (FLO-MIN106D) following the manufacturers instructions.