design: It is imperative to explore the relationship between strigolactone phytohormone and nitrogen deposition of plants. In the present study, the effects of strigolactone on genetic responses of Torreya grandis, under simulated nitrogen deposition were explored using high-throughput sequencing techniques. The concentration of nitrogen used was 160 kg N ha 1 yr 1. The amount of spraying per year was converted into the amount for each month. Strigolactone treatments comprising spraying of 10 M of GR24 solution to the shoots of T. grandis seedlings.