design: Pacific Biosciences protocol 20 kb Template Preparation Using BluePippin Size-Selection was used to create a sequencing library from seven micrograms of genomic DNA. Reagents were supplied in the SMRT bell Template Kit (Pacific Biosciences). The DNA was sheared using a G-tube (Diagenode).  Size selection of 15-50kb was determined by the molecular weight of the input DNA as determined by pulse filed gel analysis.