design: We used parallel PCR amplification of 48 different DNA barcodes to combine taxon-general (e.g., orders, families, genera) and taxon-specific (e.g., genera, species) screening methods into a single assay. Target amplification was performed by the University of Illinois at Urbana-Champaign Roy J. Carver Biotechnology Center using a Fluidigm 48.48 Access Array.