design: Strains streaked on BHI agar from -80C glycerol stocks and incubated at 37C overnight. A single colony was inoculated into 1 ml BHI, incubated at 37C overnight, pelleted, and stored at -20C until extraction for genomic DNA with the Qiagen DNeasy Blood and Tissue kit. gDNA was eluted in Qiagen Buffer AE. Nextera XT library creation and Illumina HiSeq 2500 high-output mode paired end 125bp were performed at the University of Minnesota Genomics Center.