design: Cells were grown in a glutamine depleted RPMI [RPMI 1640 1X from Corning (15040 CM)], completed with 15% FBS containing RPMI at 37 and 5% CO2. The lines were passaged 3 times, maintained at 8105 cells, and grown to a concentration of 1106 cells per mL before RNA extraction. Cells from each line were spun down and pelleted at 200g at 500 RPM at 4C for 2 min, washed with cold phosphate-buffered saline (PBS), and spun down again in the same way before aspirating the PBS. RNA was extracted using the miRNeasy kit (Qiagen) according to manufacturer instructions, including the DNase step to remove potentially contaminated genomic DNA. cDNA synthesis was performed in triplicate, with each reaction starting with 800-1000ng of total RNA. The samples were sequenced on a Pacific Biosciences (PacBio) Sequel II sequencer using 4 SMRTcells. Circular consensus reads (CCS) were generated using pbccs (v 6.0.0). Primers were removed, samples were demultiplexed, and fastqs were generated using lima (v2.0.0) and bamtools (v2.5.1).