design: The alive liver cells of three mice in each group (WT and ISXTG) were dissociated according to the above procedure and pooled for further analysis. For a target recovery of 12,000 single cells, 15,000 live cells were loaded into the 10 Genomics Chromium Single Cell chips. Next, libraries were prepared using the Chromium Single Cell 3 GEM Library & Gel Bead Kit v3 (10 Genomics) according to the manufacturer's instructions and sequenced on an Illumina Novaseq 6000 with a sequencing depth of at least 100,000 reads per cell and 150-bp (PE150) paired-end reads.