design: For whole-genome sequencing, large-insert SMRTBell libraries (>20kb), selected using a SageElf (Sage Science, Inc., Beverly, MA, USA), were prepared according to standard manufacture protocols and sequenced at the BYU DNA Sequencing Center (Provo, UT, USA) using P6-C4 chemistry on a Sequel II instrument (Pacific BioSciences, Menlo Park, CA, USA)