design: Allogeneic direct limbal stem cell transplantation was conducted on the right eye of the patient with gelatinous drop-like corneal dystrophy (GDLD). Ten months later GDLD patient undergone keratoplasty on the right eye and cornea sample was obtained. RNA was isolated from this cornea sample and used for transcriptome construction. This cornea sample contains donor and host cells, so the results of transcriptome analysis should be treated with care. RNA was extracted using RNeasy Micro Plus Kit (Qiagen) according to the manufacturer's protocol for tissues. Traces of DNA were removed with TURBO DNA-free Kit (ThermoFisher Scientific). The entire RNA sample was used for transcriptome library construction with NEBNext Ultra II Directional Library Prep Kit for Illumina (New England Biolabs) and Multiplex Oligos for Illumina (96 Indexes) (New England Biolabs). Ribosomal RNA was depleted using NebNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs). The library was sequenced on the Illumina HiSeq 2500 instrument with 2 125 cycles using HiSeq SBS Kit v4 (Illumina).