design: The single nuclei were extracted from 30 to 50 brains of the same Monomorium pharaonis adult phenotype and the snRNA-seq library was constructed from the DNBelab C Series Single-Cell Library Prep Set (MGI) according to the manufacturer's protocol. Read 1 was 30-bp in length and contained a 10-bp cell barcode 1, a 10-bp cell barcode 2, and 10 bp unique modecular identifier (UMI). Read 2 was 100-bp in length and represented the transcript sequence.