design: 50ng DNA was sheared into 300 base pair. The fragmented DNA was then uniquely indexing tagged, amplified by Kapa LTP Library Preparation Kit (KAPA Biosystems, Roche7961880001) and NEBNext Multiplex Oligos for Illumina (New England Bio-Labs, E6444S).  The library was pooled with other libraries and sequenced on the Illumina MiSeq platform with paired-ends for 2 x 150 cycles.  A MiSeq Reagent Kit v3 was used.  (MS-102-3003).