design: There were four nitrogen levels in the soil:16 kg667m-2 pure nitrogen (reducing nitrogen by 55.6%, treatment A), 26 kg667m-2 pure nitrogen (reducing nitrogen by 27.8%,treatment B), 36 kg667m-2 pure nitrogen (conventional fertilization,treatment C), no nitrogen application(CK). The rhizosphere soil and samples of 0-20cm, 20-40cm no rhizosphere soil were sequenced by 16S rRNA amplification, and the community structure of soil bacteria under different nitrogen reduction treatments was analyzed by IonS5TMXL high-throughput sequencing platform.