design: A minimum of 500 ng of DNA were generated for each sample. The reactions were processed using a Tetrad thermal cycler (Bio-Rad) at the following conditions: 94C for 20 s; 63C for 30 s; and 68C for 8 min for 24 cycles. Following PCR amplification, sample reactions were pooled together and concentrated using an ISS110 Speedvac system (Thermo Fisher Scientific) to evaporate excess water using a vacuum. All samples were then purified using AMPure XP beads (Beckman Coulter) according to the manufacturers manual.