design: Library construction and total RNA sequencing was performed by LC Science, USA. For Total RNAsequencing, ribosomal RNA was removed, followed by fragmentation with divalent cation buffers inelevated temperature. The sequencing library was prepared following Illuminas TruSeq-stranded-total-RNA-sample preparation protocol. Paired-ended sequencing was performed on IlluminasNovaSeq 6000 sequencing system.