design: DNA was extracted from individual whole mosquitoes using QIAGEN DNEasy Blood and Tissue Kits following manufacturer's instructions. DNA was eluted and stored at -20oC, before being sent for sequencing at the University of Texas Medical Branch, Next Generation Sequencing Core facility using the NexteraDNA Flex protocol for library construction, and the samples sequenced in paired-ended format on a NextSeq550 machine at a length of 150 base-pairs.