design: Total RNA of rumen papillae was extracted with TRIzol reagent (Life technologies,US, Cat#74106) following manufacturers protocol, and was assessed by Agilent 2100 bioanalyzer (Agilent Technologies, US) with the RNA 6000 Nano Kit (Agilent Technologies, US) for the integrity as well as concentration. Complementary DNA (cDNA) was synthesized by the VAHTS mRNA-seq v2 Library Prep Kit (NR601-01, Vazyme Biotech Co., Ltd, Nanjing, China) following protocol and was used to construct a library for sequencing. The OD260/OD280 values were  1.9, and the RIN (RNA Integrity Number) values were 8.0. The RNA-Seq, libraries were sequenced on an Illumina HiSeq 4000 platform (Illumina, Inc., San Diego, CA 92122, United States) at Berry Genomics Co., Ltd. (Beijing, China).