design: In this experiment, the two terminal sequencing mode of Illumina hiseq sequencing platform is used to conduct high-throughput sequencing on multiple samples. According to the distribution characteristics that the low quality fraction of Illumina sequencing data is concentrated at the end, the connector sequence fragments and low-quality fragments are dynamically removed from the 3 'end of the sequencing data by skewer software, and the quality control analysis and statistical Q20 of the preprocessed data are carried out by fastqc software, Base ratio of q30.fast