title: GSM6365606: A549-miR-19a2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from NSCLC cells or EVs using QIAzol and the miRNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA quantity and quality were determined using Agilent Bioanalyzer (Agilent Technologies). RNA library preparation with polyA selection and Illumina HiSeq 2 × 150 bp sequencing was performed by GeneWiz Inc. Image analysis and base calling were conducted by the HiSeq Control Software (HCS).