title: GSM6009025: CTCF ChIP-seq on embryonic 16.5 day mouse forebrain, biological replicate 1; Mus musculus; ChIP-Seq
library_construction_protocol: ~30 mg fixed tissues were thawed on ice and the nuclei preparation was performed using truChIP® Chromatin Shearing Kit (Covaris) following the manufacturer's instruction. The lysed nuclei were sheared using Covaris M220 with following setting: Setting: power, 75 W; duty factor, 8%; cycle per burst, 200; time, 12 min; temp, 7 °