title: Bacterial community in 500 mg kg-1 Cu-spiked rhizospheric soil of Elsholtzia eriocalyx
design: Microbial DNA was extracted using the HiPure Soil DNA Kits (or HiPure Stool DNA Kits ) (Magen, Guangzhou, China) according to manufacturers protocols. The full length 16S rDNA were amplified by PCR 95 C for 2 min, followed by 35 cycles of 95 C for 30 s, 60 C for 45 s, and 72C for 90s, with a final extension 72C for 10 min  using primers 27F: AGRGTTYGATYMTGGCTCAG 1492 R: RGYTACCTTGTTACGACTT [44] Listed in table 1 . The PCR reaction was carried out in a 50 L reaction volume with TransGen High-Fidelity PCR SuperMix (TransGen Biotech, Beijing, China), 0.2 M forward and reverse primers, and 5 ng template DNA. Amplicons were evaluted with 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturers instructions. Sequencing libraries were generated using SMRTbell TM Template Prep Kit (PacBio, Menlo Park, CA, USA) following manufacturers recommendation. The library quality was assessed with Qubit 3.0 Fluorometer (ThermoFischer Scientific,USA) and FEMTO Pulse system (Agilent Technologies, Santa Clara, CA, USA). The libraries were sequenced on the PacBio Sequel platform.