title: ITS2 metabarcoding of Melipona quadrifasciata forager bees
design: ITS2 and 16S amplicons were obtained using universal primers barcoded at their 5' ends and then pooled for building libraries with the TrueSeq Nano Library Preparation kit. Paired-end sequencing (2  300 nt) was performed with the MiSeq Reagent kit v3.