title: GSM5815972: Zac1_S4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from frozen pellets using the NucleoSpin kit (Macherey Nagel) and quantified using a Nanodrop 2000. Dnase I treatment was performed as part of the protocol. RNA-seq libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit and NEBNext Mulitplex Oligos for Illumina (NEB).