title: GSM5662214: MOCK; Mus musculus; RNA-Seq
library_construction_protocol: From single-cell suspensions of mock and IAV-infected lungs at 7 and 14 days after infection, CD8 T cells were FACS sorted by positive staining with anti-mouse CD8-APC and CD3-FITC using BD Aria II flow cytometer sorter. The purity of CD8+ T cells was confirmed by flow cytometry.2x106 cells were resuspended in media containing 10% DMSO and 20% FBS and allowed to slow freeze in Mr Frosty RNA libraries were prepared with the Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.