title: GSM5537824: HCT116 shNC+Dox rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using RNAzol. Three microgram total RNA was used for polyadenylated RNA enrichment by using VAHTS mRNA Capture Beads (Vayzme) following the manufacture's protocol. Standard protocol of NEBNext Ultra ™ II Directional RNA Library Prep Kit was followed for mRNA enrichment and library preparation.