title: Could we engineer synthetic and functionally programmable plant microbiome that can through lifeline to rice plants against pathogenic infection?
design: Fungal ITS of rRNA were PCR-amplified using ITS1f/ITS2 primer pair (ITS1F: 5- GTGAATCATCGAATCTTTGAA-3; ITS2R: 5-TCCTCCGCTTATTGATATGC-3). Sequencing was performed on the Illumina MiSeq platform using the Illumina MiSeq reagent kit v3 2x 300bps paired-end chemistry as per manufacturers instructions.