title: GSM6234567: HiChIP EndoC 3cyt-hi 72h; Homo sapiens; OTHER
library_construction_protocol: Cells were cross-linked with 1% formaldehyde for 15 minutes with shaking at room temperature, followed by a 5-minute quenching step with 1.25 M glycine/PBS. Cross-linked EndoC-βH1 cells in both control and cytokine-treated conditions were washed three times with ice-cold PBS and collected from the dish with a cell scraper. Cells were then pelleted, and flash frozen with liquid nitrogen. HiChIP assays were performed by Arima Genomics using the HiC+ protocol with a H3K27ac antibody, and libraries were sequenced on an Illumina NovoSeq with 150 bp paired end reads.