title: GSM5537631: M1AL3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated and sent to Quickbiology, Pasadena, CA, USA where RNA-seq and data analysis were carried out. RNA integrity was checked using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and samples with clean rRNA peaks (RIN > 7) were used for further experiments. Library for RNA-seq was prepared according to KAPA stranded mRNA-Seq poly-A selected kit with a 201–300 bp insert size (KAPA Biosystems, Wilmington, MA, USA) using 250 ng total RNA as input. Final library quality and quantity were analyzed using an Agilent Technologies Bioanalyzer 2100 and Life Technologies Qubit 3.0 Fluorometer (Carlsbad, CA, USA). 150 bp paired-end reads were sequenced on an HiSeq 4000 (Illumina, San Diego, CA, USA).