title: GSM5258184: RSV-dgd; Nicotiana benthamiana; Rice stripe tenuivirus; OTHER
library_construction_protocol: For degradome-seq, mRNA was enriched using oligo-d(T) magnetic beads. The enriched mRNA was mixed with biotinylated random primers and were ligated to 5' adaptors. First-strand cDNA was reverse-transcribed from ligated mRNA products and then amplified with PCR. Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure