title: GSM5414262: CMi_EV_3; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from EV samples (N=3 biological replicates corresponding to EV preparations from three independent cultures) using Norgen Biotek Exosomal RNA Isolation Kit (Cat.58000). microRNA library preparation was performed by PCR amplification using Norgen Biotek Small RNA Library Prep Kit (Cat. 63600) and library quality control was achieved using Bioanalyzer to estimate library size and concentration. Libraries were denatured and diluted to the required concentration and then applied onto the suitable flowcell and sequenced using Illumina NextSeq 500 sequencing platforms Small RNA-seq