title: Earthen pit manure storage - location 3 (EP3); surface; replicate 1
design: DNA was extracted using Qiagen Fast DNA Stool Mini Kit. Amplification of 16S rRNA hypervariable region 4 (V4) using 515F and 806R primer set (Parada et al, 2016). Library was prepared according to Illumina Metagenome MiSeq sequencing protocol and sequenced using Illumina MiSeq.