title: GSM6381691: HTR5-GFP_G24h_2_input; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Materials were ground with liquid nitrogen into fine powder and immediately fixed with 1% formaldehyde. Nuclei were extracted and mononucleosomes were generated with micrococcal nuclease (MNase) (Sigma, N5386) digestion as previously described. Immunoprecipitation was conducted with anti-GFP (Thermo Fisher Scientific, A-11122) or anti-H2A.Z. After antibody incubation, Protein A Dynabeads (Thermo Fisher Scientific, 10002D) were added to collect the immunocomplexes. After washing, elution, reverse cross-linking and DNA purification, recovered DNA was subjected to library preparation with VAHTS universal DNA library prep kit for Illumina (Vazyme, ND607) according to the manufacture's instruction