title: GSM6381682: Col_MS_1 Bisulfite-seq; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted with Quick-DNA Plant/Seed Miniprep Kit (ZYMO, D6020). 200ng fragmented genomic DNA (200bp-500bp) was used for library preparation with VAHTS universal Pro DNA library prep kit for Illumina (Vazyme, ND608). NEBNext Multiplex Oligos for Illumina (NEB, E7535) were used for adapter ligation. After purification with VAHTS DNA Clean Beads (Vazyme N411), bisulfite conversion was performed with EZ DNA Methylation-Gold Kit (ZYMO, D5005), followed by DNA purification and PCR amplification.