title: GSM6203859: RNA-seq, C42B, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: The human prostate cancer C42B cells were obtained from ViroMed Laboratories (Minneapolis, MN, USA) Cells were grown at 37 °C in 5% CO2. It was grown in RPMI1640 culture medium, and supplemented with 10% fetal bovine serum (Gibco by Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin and streptomycin. All cell stocks were authenticated at the USC Norris Cancer Center cell culture facility by comparison to the ATCC and/or published genomic criteria for that specific cell line; all cells were documented as free of mycoplasma. RNA was extracted with Trizol reagent. RNA-seq libraries were prepared with KAPA Stranded mRNA-Seq Kit with KAPA mRNA Capture Beads (Cat # KK8421; Kapa Biosystems, Woburn, MA, USA) then sequenced on an Illumina NextSeq 500 with 75 bp single end reads.