title: GSM5748016: Apoe -/- phagoctyic pooled sample 2; Mus musculus; RNA-Seq
library_construction_protocol: Microglia were isolated as CD11b+/Ly6c–/Fcrls+ cells, and phagocytic versus non-phagocytic microglia were further sorted from the Fcrls+CD11b+ population by detection of Alexa488. 5-6 retinas were pooled per sample according to sex and genotype to increase yield. 100-500 phagocytic cells were collected per sample and normalized to 100 to 300 cells per 5 μl of TCL buffer for RNAseq. RNA libraries were prepared for sequencing using standard Illumina protocols