title: GSM6152907: Control, rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Mouse SSCs were cultured in the presence or absence of EPZ5676 for 7 days. SSCs were resuspended at 1 million per ml in PBS and crosslinked with formaldehyde (INSERT) at a final concentration of 1% for 10 min at room temperature (RT). The crosslinking reaction was stopped by adding glycine to a final concentration of 0.125 M for 5 min at RT. All following buffers were supplemented with protease inhibitors (Complete Mini, Roche or Halt, Thermo Fisher Scientific). Cells were washed twice in ice cold PBS and lysed in ChIP lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl, pH8.1). NEBNext Ultra II DNA Library Prep kit for Illumina (E7645S, NEB) by following the manufacturer's instructions