title: GSM5454892: DTC1_siCTRL_96h_ATAC_2; Homo sapiens; ATAC-seq
library_construction_protocol: 75 000 cells were collected and re-suspended in lysis buffer to swell cytoplasm. The nuclei preparation was lysed DNA was tagmented with Tn5 Transposase (Nextera DNA Sample prep kit, Illumina #15028212) for 30 min and purified. Sequencing libraries were generated by 12 cycles of PCR amplification using NEBnext PCR mix (New England Biolabs) and custom Nextera primers.