title: GSM5747738: SP020L; Homo sapiens; RNA-Seq
library_construction_protocol: Resected lung tissues were processed in the BSL2+ laboratory and digested with Collagenase IV (100 U/ml) and Benzonase (50 U/ml) in RPMI 1640 medium without serum for 45 min at room temperature with rocking. Debris was removed using Dead Cell Removal Kit (Miltenyi Biotec). Cells were collected by centrifugation, resuspended in 1x DPBS on ice, and passed through a 40mm strainer. Cell viability measured by trypan blue staining was above 75%. Single cell samples were processed using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3 and, after cDNA amplification, removed from BSL2+ laboratory for subsequent library construction and sequencing.