title: NextSeq 500 paired end sequencing; RNA-seq of human breast cancer cell line SUM190 treated with Nectin4 ADC
design: RNA-seq of human breast cancer cell line SUM190 treated with Nectin4 ADC
library_construction_protocol: The SUM190 BC cell line was kindly provided by Dr Ethier (https://sumlineknowledgebase.com/) Tumor RNAs were extracted from sensitive and resistant pre-clinical models and controlled on Agilent Bioanalyzer (Agilent Technologies, Massy, France) cDNA was synthetized by random hexamer primers and reverse transcriptase (Superscript II, Life Technologies). Next, end repair and 3â€² ends adenylation of the fragments were performed. Bar-coded adapters were ligated to the cDNA fragments and a PCR reaction was performed to produce the sequencing libraries. The quality check of libraries and quantification were performed using Tape station High sensitivity D1000