title: GSM6149761: epithelial ciliated cells, Cal09-sfGFP infected mice, 3 dpi, bystander; Mus musculus; RNA-Seq
library_construction_protocol: Mice were anesthetized with ketamine-xylazine prior to cervical dislocation. The lungs were perfused with PBS then inflated with 1.5 mL of 1:1 mixture of dispase and 1% low-melt agarose. Lungs were removed then incubated in dispase for 45 minutes at room temp. Lungs were finely minced with a razor blade then pipetted vigorously using a 5 mL serological pipette before being filtered (70 um filter). Red blood cells were lysed with 1x RBC lysis buffer and then remaining cells were washed with 2%BSA in PBS. Single cell suspensions from 2 mice (of the same infection status and timepoint) were pooled prior to sorting on a BD DiVA. Sorted cells were prepared for library generation (10x Genomics) according to manufacturers protocols.