title: GSM5815323: ascr50-1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was extracted with Qiagen RNeasy Mini Kit (Cat. No. 74104) and on-column DNase digestion done with Qiagen RNase-Free DNase (Cat. No. 79254) according to the manufacturer's protocols. Three independent samples were prepared and analyzed for each condition each time the experiment was performed. 1μg of total RNA was used in library preparation with NEBNext® UltraTM II Directional RNA Library Prep with Sample Purification Beads Kit for Illumina (NEB#E7765). NEBNext® UltraTM II Directional RNA Library Prep with Sample Purification Beads Kit for Illumina (NEB#E7765)