title: amplicon (16S V4V5) of Ambystoma maculatum: egg capsules
design: We sampled fluid from egg capsules of Ambystoma maculatum in a pond in Nova Scotia in 2019 and that with two other ponds in 2020, and subjected extracted DNA to 16S amplicon sequencing (V4-V5) on an Illumina MiSeq at the IMR at Dalhousie University (https://imr.bio/protocols.html).