title: GSM4806158: 1aK8_10min_BL_3; Phaeodactylum tricornutum; RNA-Seq
library_construction_protocol: Cells were harvested and immediately frozen in liquid nitrogen. RNA was isolated from filtered cells using PeqGOLD RNApure and PeqGOLD RNA extraction kits (VWR). Residues of DNA were removed by an on-column digest with the PeqGOLD DNase I digest kit (VWR). RNA integrity and fragment size were analyzed on a 2100 Bioanalyzer (Agilent). Libraries were prepared from 1 µg RNA using the TruSeq RNA Sample Prep Kit v2 (Illumina), and quantified with a Qubit 2.0 (Invitrogen).  Samples were multiplexed  and sequenced in paired-end mode (150 bp read length) on an Illumina HiSeq 2000 and HiSeq 3000 platform, respectively.