title: GSM5538777: MF17_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: Skin biopsies were minced and digested enzymatically (Whole Dissociation Skin Kit, Miltenyi Biotec) for 2 hours at 37°C and further dispersed using the Miltenyi gentleMACS Octo Dissociator. Normal skin biopsies were hashed together 2 at a time per protocol: https://www.biolegend.com/en-us/protocols/totalseq-b-or-c-with-10x-feature-barcoding-technology Normal Sample 1 and 2 are labeled with HTO1 and HTO2 respectively. Normal sample 3 and 4 are labeled with HTO9 and HTO10 respectively. Experimental procedures followed established techniques using the Chromium Single Cell 5' Library V1 kit (10x Genomics). Experimental procedures followed established techniques using the Chromium Single Cell 5' Library V1 kit +V(D)J Enrichment Kit, Human T Cell (10x Genomics).