title: GSM6204322: Genome_Wide_CRISPR_Screen_K562_Unselected; Homo sapiens; OTHER
library_construction_protocol: Cell pellets were lysed and genomic DNA was extracted using the QIAamp DNA Mini Kit (Qiagen) and quantified by Qubit (ThermoScientific). gRNAs amplicons were amplified by PCR using TaKaRa ExTaq® DNA Polymerase (Takara) to add Illumina sequencing adapters and multiplexing barcodes. Amplicons were quantified by Qubit and Bioanalyzer (Agilent), multiplexed, and sequenced on an Illumina NextSeq 500 to obtain 75b.p. single-end reads.