title: GSM6149735: KF-11mo; Mus musculus; RNA-Seq
library_construction_protocol: Spontaneous pancreatic tumor digested to single cell suspension and submitted for single cell RNA sequencing Samples were mechanically and enzymatically digested (Collagenase V; 1 mg/mL in RPMI) under constant agitation. Single cell suspensions were obtained by filtering consecutively through 500 µm, 100 µm, and 40 µm cell strainers. Dead cells were removed using MACS Dead Cell Removal Kit (Miltenyi Biotec). Single cell cDNA libraries were prepared and sequenced using the 10x Genomics platform at the University of Michigan Advanced Genomics Core.