title: GSM5026600: K562_Rep2_SMARCA4; Homo sapiens; ChIP-Seq
library_construction_protocol: K562 cells  were cross-linked using  2 mM disuccinimidyl glutarate for 30min then in 1% formaldehyde for 10 min at room temperature. Chromatin was isolated using a ChIP-qPCR Kit (Chromatrap, cat. no. 500117) und sonicated using a Bioruptor Plus (Diagenode) to give a fragment distribution of 150-400 bp. ChIPed DNA was subjected library preparation using a NEBNext Ultra DNA Library Prep Kit for Illumina (NE Biolabs) following the manufacturer's protocol . Final products were sequenced by Illumina Nextseq 500 platform.  For further details see methods section in the associated manuscript.