title: GSM6234697: DRG_CD8--DRG_rep3; Mus musculus; RNA-Seq
library_construction_protocol: 1×104 naive TRPV1Cre::QuASR2fl/wt DRG neurons were co-cultured with 1 × 105 B16F10-mCherry-OVA overnight in T-cell media (supplemented with 0.05 ng/μL NGF (Life Technologies, #13257-019), 0.002 ng/μL GDNF (PeproTech, #450-51-10). One day after, 4 ×105 stimulated OVA-specific CD8+ T-cells under Tc1 condition were added to the co-culture. After 48h, the cells were detached and TRPV1 neurons (CD45- eGFP+ mCherry-), B16F10-mCherry-OVA (CD45- eGFP- mCherry+), and OVA-specific CD8+ T-cells (eGFP- mCherry- CD45+ CD3+ CD8+) were FACS-purified using a FACSAria IIu cell sorter (Becton Dickinson). RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina, San Diego, CA) following the manufacturer's instructions.