title: GSM6234285: ChIP-seq input for H3K27ac in Pcca-/- female 8-week-old mouse heart; Mus musculus; ChIP-Seq
library_construction_protocol: 8-week-old mouse hearts were fixed for 10 min at room temperature with 1 % formaldehyde in modified AMVM isolation solution containing 0.5 mM EGTA (solution pH 7.4 at room temperature) in Langendorff mode. Fixed ventricles were minced and rinsed twice in PBS, then lysed in ChIP lysis buffer (1 % SDS, 10 mM Tris-HCl pH 8.0, 1 mM EDTA) by homogenisation (Qiagen TissueRuptor II). Lysates were pooled with fixed, sonicated Drosophila S2 cells at a ratio of 5:1 mouse:Drosophila cells, followed by IP. DNA libraries were made using the NEBnext Ultra II DNA library preparation kit for Illumina (Cat no. E7645).