title: GSM5260274: A20_Veh; Mus musculus; RNA-Seq
library_construction_protocol: Single cell RNA libraries were generated from A20 tumors derived from tumor-bearing Balb/c mice 8 h after treatment with vehicle or 7.5 mg/kg TAK-981 using the Chromium Single Cell 5' kit (10X Genomics) following Dead Cell Removal (Miltenyi). Cells were loaded onto the Chromium Controller (10 X Genomics) to target capture of ~3,000 GEMs (Gel Bead-in Emulsions) per sample for downstream analysis, following the manufacturer's specifications. The sequencing libraries were evaluated for quality on the Agilent TapeStation (Agilent Technologies), and quantified by using Qubit 2.0 Fluorometer (Invitrogen) and pooled libraries were quantified using qPCR (Applied Biosystems) prior to loading onto an Illumina (Illumina) sequencing platform.