title: scRNA-seq of Mus musculus: EPCAM+ lung epithelial cells, TET1 KO, saline treated
design: For 10X Genomics single cell RNA sequencing, cells were brought to a concentration of about 1000 cells/L using the resuspension buffer. 8000 cells were targeted for cDNA preparation and put through the 10X Genomics Chromium Next GEM Single Cell 3 v3.1 pipeline for library preparation. After cDNA preparation and GEM Generation and clean-up, cDNA was quality checked and quantified on an Agilent Bioanalyzer DNA High Sensitivity chip at a 1:10 dilution. Successful traces showed a fragment size range of approximately 1000-2000bp. 12 indexing cycles for library was used. The quality of indexed libraries was checked on an Agilent Bioanalyzer DNA High Sensitivity chip at a 1:10 dilution. Successful libraries had fragment size distributions with peaks around 400bp. Libraries were sequenced using 4 Illumina Hiseq lanes (Paired-end 75bp)