title: GSM5026616: HSF V30M TTR mice A1; Mus musculus; RNA-Seq
library_construction_protocol: RNA from sciatic nerves was isolated with RNeasy Mini columns, following the manufacturer′s instructions (Qiagen, Hilden, Germany, catalog #74804). First-strand complementary DNA (cDNA) was synthesized using the SuperScript double-stranded cDNA Kit (Invitrogen). Subsequently, the cDNA samples were delivered to Bioinf2Bio Company for RNA sequence analysis. RNA libraries were prepared for sequencing using standard Ion Torrent protocols