title: GSM6149782: SHEDs biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using TRIzol (Invitrogen). 1 ug of total RNA was used for the construction of sequencing libraries. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in First Strand Synthesis Reaction Buffer(5X). The library fragments were purified with AMPure XP system (Beckman Coulter, Beverly, USA).