title: GSM5508402: Untagged_EtOH_Rep2 [MNase-seq]; Mus musculus; MNase-Seq
library_construction_protocol: Medium was aspirated and cells were washed with DPBS. DPBS was aspirated and cells weretrypsinized. Cells were pelleted, washed with DPBS, crosslinked with 1% formaldehyde for 15 minutes at room temperature, and quenched with 0.5M glycine for five minutes. Crosslinked cells were pelleted, lysed in hypotonic buffer and pre-incubated with calcium, then digested with 30U MNase for five minutes and chelated with EDTA.