title: DNA-Seq of Pseudomonas fluorescens
design: The qualified DNA samples detected by electrophoresis were randomly broken into fragments of about 354bp length by Covaris ultrasonic crusher, and the whole library was prepared by terminal repair, A-tail, sequencing joint, purification and PCR amplification. After the construction of the library is completed, the library is initially quantified by Qubit2.0 and diluted to 2ng/uL, and then the insert size of the library is detected by Agiient2100. After insert size meets the expectations, the effective concentration of the library is accurately quantified by Q-PCR method to ensure the quality of the library.