title: GSM6504874: Org26 H9 0hr rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were homogenized in Trizol (Invitrogen), and total RNA was purified using the RNeasy Micro Kit (Qiagen) with DNase I digestion. Ribosomal RNA was depleted with the NEBNext rRNA Depletion Kit. Libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina and verified using the Bioanalyzer RNA Nano Assay (Agilent). ﻿After DNase treatment and rRNA depletion, strand-specific and single-end cDNA libraries were generated using the PE RNA-seq library kit (Illumina)