title: GSM6365585: iNSC1.Input; Mus musculus; MeDIP-Seq
library_construction_protocol: Total RNA was extracted from SSCs and iNSCs with TRIzol reagent (Invitrogen, 15596026), and then mRNA was purified using a Dynabeads mRNA Purification Kit (Invitrogen, 61006) according to the manufacturer's instructions and fragmented into ~200 nt fragments using the RNA Fragmentation Reagents (Invitrogen, AM8740). Subsequently, the fragments were incubated with an m6A antibody-Pierce protein A/G beads mixture (Abcam, ab151230; Thermo Fisher Scientific, 88802), and then the eluted m6A mRNA was further purified using an RNA cleanup kit (Zymo Research, R1013). The purified m6A mRNA was used for library construction with the NEBNext Ultra II Directional RNA Library Prep Kit. The mRNA was sequenced with Illumina NovaSeq 6000 following the manufacturer's protocols.