title: GSM5747748: Input Tbx3 FL bud E9.75-E10.25 replicate 1; Mus musculus; ChIP-Seq
library_construction_protocol: The protein/DNA complexes were eluted using an SDS buffer (1% SDS, 50 mM Tris pH 8.0, 10 mM EDTA) at 65oC overnight. Samples were treated for 15 min RT with RNase A and then with Proteinase K for one hour at 56oC. Finally, the DNA was purified using MicroChip Diapure columns (Diagenode). Libraries were prepared using MicroPlex Library preparation kit V2 (Diagenode) according to the manufactures instructions and 80 bp paired-end reads were sequenced using the Illumina Nextseq 500.