title: GSM6204185: Syn3A cells, colony A2, endpoint evolution; synthetic bacterium JCVI-Syn3A; RNA-Seq
library_construction_protocol: Genomic DNA was isolated using bead agitation in 96-well plates Total RNA isolation, rRNA removal, and sequencing library preparation was performed as previously described  (Chen et al. 2021)  removal, and sequencing library preparation was performed as previously described  (Chen et al. 2021) Libraries were run on an Illumina NextSeq, raw sequencing reads were mapped using Bowtie