title: GSM5258529: B6585; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated following the protocol of Chomczynski and Sacci (Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.). Poly-A capture was performed on the samples to purify the mRNA, and directional mRNA libraries were made using the PrepX mRNA Library Kit.