title: Genome sequencing of bacterial plant pathogens: WGS of Pseudomonas syringae pv. aptata
design: DNA libraries were prepared using the Thermo Fisher IonXpress Plus Fragment Library Kit, according to the manufacturer's protocol. The kit uses an enzyme-based random fragmentation method followed by ligation of adapters and amplification. Library quantity was assessed with Qubit ThermoFisher, USA). Template preparation involves clonally amplified Libraries on the Ion Chef System by emulsion PCR of library molecules captured on beads. The Ion Chef System performs all template preparation steps, including creating the emulsion mixture, performing the PCR, carrying out the post-PCR purifications, and finally loading the purified templated beads onto the Ion S5 chips (Ion 540 Chip). Libraries on prepared chips were then sequenced on a Thermo Ion S5 XL sequencer (ThermoFisher, USA). Read lengths of 200 bp were produced with Ion 540 Chip which generates around 60-80 million reads.