title: GSM5261175: Pulldown Med15 LibAB replicate2; synthetic construct; OTHER
library_construction_protocol: N/A The cDNA region encoding the variable protein fragment was PCR-amplified for 11-20 cycles  using Phusion polymerase with PCR primers that added flanking sequences corresponding to the standard Illumina sequencing primers. After a column cleanup, a second PCR was performed with primers that added sample-specific barcodes and the P5 and P7 sequences for Illumina sequencing. OTHER:sequncing of designed oligo library