title: GSM5258386: SYSMH2; Homo sapiens; RNA-Seq
library_construction_protocol: According to the manufacturer's protocol, the Chromium Single Cell 3'Reagent v3 Kit (10X Genomics, USA) was used to prepare a barcoded scRNA-seq library. The single-cell suspension was loaded onto a chromium single-cell controller instrument (10×genomics, USA) to produce single-cell gel beads in the emulsion. After generating Gel Beads-in-emulsion (GEM), reverse transcription was performed to generate full-length barcode genes. The purified cDNA was amplified by PCR using a Qubit to detect the concentration, and an Agilent 2100 was used to detect the fragment size. Each library was sequenced on the HiSeqXTen platform (Illumina).