title: GSM5969933: astrocytes, LA3; Mus musculus; RNA-Seq
library_construction_protocol: hippocampus was extracted and dissolved by collogenase and DNAse I for 30 mins, debries and mylins were removed by percoll, single cell suspension was stained with GLAST- APC antibody fro 20min and DAPI for 10 min, GLAST+/DAPI- cells were collected by BD fusion as live astrocytes. total RNA was extrated by Qiagen RNeasy Micro kit. RNA libraries were prepared for sequencing using standard Illumina protocols