title: GSM5027408: mT3-2D-GFP FACS cells from WT tumors; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Micro Kit (Qiagen). RNA concentration and quality were assessed using an Agilent BioAnalyzer (RINs >7). Equal amounts of RNA from each corresponding group were combined as one sample (1 WT sample, 1 SCID sample, and 1 cell line). Indexed, paired-end sequencing libraries were prepared from 1.0 µg total RNA using the Illumina TruSeq Stranded mRNA Library Preparation Kit (polyA cDNA synthesis) in the Genomics and Epigenomics Shared Resource (GESR) in the Lombardi Comprehensive Cancer Center at Georgetown University Medical Center. Libraries were sequenced on an Illumina NextSeq550 using PE 75 bp (150 cycle) chemistry to a mean depth of 40M reads per sample