title: UCE target enrichment of colinus_virginianus_godmani_AMNH_768798
design: We used 250 ng of sheared DNA in 25 uL volume to prepare dual-indexed genomic libraries of each sample at _ volume reactions using the KAPA Hyper Prep library preparation kit and custom indexes (Glenn et al., 2019). __We combined the libraries into pools containing between six and eight samples for enrichment, and we kept tissues and toepads in separate pools. We enriched each library pool for 5,060 UCE loci using a MYbaits_Tetrapods-UCE-5K kit. After enrichment, we performed 16 cycles of PCR recovery, removed adapter-dimers using a Qiagen GeneRead Size Selection Kit, and collected paired-end 150 bp sequence data from an Illumina HiSeq 3000.