title: GSM5026367: P5-IL1B-6; Mus musculus; RNA-Seq
library_construction_protocol: Tissue dissociation:  NTDK miltenyi Cell enrichment:  MACS Enrichment markers:  CD11B microbeads (Miltenyi 130-049-601) Single cell isolation:  droplet-based cell isolation Single cell entity:  whole cell Single cell quality metric:  Cell encapsulation follows Poisson distribution (lambda 0.1) => flow rate control with syringe pump and Droplet size and bead loading control with droplet dye and laser detection + high speed camera (Phantom) Single cell identifier:  bc*_1 Single cell isolation protocol:  droplet-based cell isolation Nucleic acid library construction protocol:  inDrops like, adapted from Zilionis et al (Nature Protocols 2017), https://doi.org/10.1038/nprot.2016.154 Nucleic acid sequencing protocol:  Illumina PE sequencing Library construction:  inDrops like, adapted from Zilionis et al (Nature Protocols 2017), https://doi.org/10.1038/nprot.2016.154 Input molecule:  polyadenylated RNA Primer:  oligodT Amplification method:  IVT (In vitro Transcription) Amplification cycles:  12 to 17 cycles (Finale PCR to incorporate Illimuna index) End bias:  3' Library strand:  both (PE sequencing) Spike in:  NO