title: RNAseq of P. astreoides: SCTLD disease bin #7
design: RNA extracted using RNAqueous-4PCR Total RNA Isolation Kit from Invitrogen (Life Technologies AM1914). Contaminating DNA and chromatin were removed using the Ambion DNase I (DNase-free) kit from Invitrogen (Life Technologies AM2222). Samples were preprocessed for mRNA enrichment using polyA tail capture. Libraries prepared using the NEBNext Ultra II RNA Library Prep Kit from Illumina. Resulting cDNA libraries were sequenced for 150 bp, paired-end sequencing.