title: GSM5640089: T3; Mus musculus; RNA-Seq
library_construction_protocol: Cells of iBAT were isolated from MMI group and T3 group and sorted by FACS to obtain APCs (Lin-CD31-652 Sca1+ cells). Live cells were resuspended in 0.04% BSA and counted using the automated cell counter (Countess). Single cell gel beads in emulsion (GEMs) generation, barcoding, post GEM-RT cleanup, cDNA amplification, and cDNA library construction were performed using Single Cell 3′ v3 chemistry (10X Genomics), according to manufacturer's protocol. The libraries were sequenced on an Illumina HiSeq X Ten platform (Illumina).