title: GSM5639532: RRBS D14 HOTAIR-OE rep1; Homo sapiens; Bisulfite-Seq
library_construction_protocol: RRBS: Genomic DNA was extracted using the GenEluteTM Mammalian Genomic DNA Miniprep Kit (Sigma) RRBS libraries were constructed using Ovation® RRBS Methyl-Seq with TrueMethyl® oxBS preparation kit. RNA-Seq: Use poly-T oligo-attached beads to purify and fragment mRNA for cDNA synthesis. Then, a single 'A' nucleotide was added to 3' end of ds cDNA, multiple indexing adaptors were ligated to 5' and 3' of the ends of ds cDNA, following with PCR amplification,