title: GSM6047028: KOPTK1_I-BET151_control_DMSO_72h_RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from live cells with TRIzol reagent followed by purification over PureLink RNA mini kit columns (Invitrogen). RNA-seq was performed using a poly A library construction protocol with strand specific cDNA synthesis and 8 cycles of PCR. Purification of polyA+ mRNA and mRNA(-) was done by Flow-through total RNA using MultiMACS 96 Separation Unit. For library Construction, 96-well Plate-based Strand-specific cDNA Synthesis using Maxima H Minus Strand Specific 96-well was used for Illumina MiSeq Sequencing.