title: GSM6588085: SD,48h; Mus musculus; RNA-Seq
library_construction_protocol: For blood sampling, each mouse was anesthetized with pentobarbital (i.p. 80 mg/kg) and taken 500 μL blood retro-orbitally which was collected in an EDTA anticoagulant tube (BD Biosciences). Total RNA was extracted from the blood samples of 3 mice in the same group with QIAamp RNA Blood Mini Kit (52304, Qiagen) following the manufacturer's instructions. The sequencing libraries were prepared with Illumina Truseq Library Prep Kits v2 (Illumina) according to the manufacturer's instructions. All libraries were guaranteed quality with quality check