title: GSM5026377: E13_MGE_multi_rna; Mus musculus; RNA-Seq
library_construction_protocol: E13.5 embryos were harvested from timed pregnant CD-1 mice  and the MGE was dissected in ice-cold Leibovitz's L-15 medium. Pooled tissue from several embryos were dissociated using the Neural Tissue Dissociation Kit (T) (Miltyeni Biotec, PN-130-093-231) according to the manufacturer's instructions. Dissociated cells were then filtered through a 70 um filter, and centrifuged at 300xg for 5 min at 4C. Nuclei then were isolated as described in the 10x Genomics demonstrated protocols 'Nuclei Isolation from Embryonic Mouse Brain for Single Cell Multiome ATAC + Gene Expression Sequencing ' for fresh tissue for input to single-cell Multiome library preparation. Single-cell multiomic (ATAC + Gene Expression) libraries were prepared on the 10x Genomics platform using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle (PN-1000285), Chromium Next GEM Chip J Single Cell Kit (PN-1000230), Single Index Kit N Set A (Pn- PN-1000212) and Dual Index Kit TT Set A (PN-1000215) as instructed by the manufacturer. Libraries were sequenced using the Nova-Seq 100 cycle kit (ATAC library) and Next-Seq 150 cycle kit (Gene Expression library) (Illumina) by Broad Institute Genomic Services. Dual scRNA/scATAC-seq