title: GSM6047831: SiOGT replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was isolated with the PureLink RNA Mini Kit (#12183018A, Thermo Fisher Scientific) following the kit instruction. RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR.