title: Whole genome sequencing ofSetosphaeria turcica f. sp. sorghi
design: 1.Genomic DNA was processed into 15-20K segments using the G-Tubes method; 2.The two ends of the single chain are connected to the positive and negative double chains respectively, resulting in a dumbbell-like structure called SMRT Bell ; 3.The library was annealed in single-chain rings and bonded to a polymerase at the base of a fixed ZMW (zero-mode waveguides);4.Computer sequencing.