title: GSM6149739: SW403_IRS2_control_bio_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the High Pure RNA Isolation Kit (Roche, Mannheim, Germany). 1000ng of total RNA was used for the construction of sequencing libraries. The libraries were prepared using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs® inc., USA). The double strand cDNA was subjected to end repair, A-base addition, adapter ligation and PCR amplification to create barcoded libraries.