title: GSM6365535: B16F10_APOE3_rep4; Mus musculus; RNA-Seq
library_construction_protocol: Fifteen days after injection, lungs were taken from mice and digested with collagenase. TO-PRO-3 low/CD45-/GFP+ tumor cells from the single cells suspension were sorted on a BD FACSAria II. RNA was isolated with the Norgen Total RNA Purification Kit with on-column DNAse treatment. RNA was then concentrated with a Zymo RNA Clean & Concentrator Kit. Libraries were constructed using QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen)