title: GSM4808358: Fetal stromal and epithelium sample pool 6 - Hashing Antibody Library; Homo sapiens; RNA-Seq
library_construction_protocol: fetal intestinal samples were digested using either EDTA chelation protocol (EpCAM+/ epithelial pools) or milteyni umbilical cord digestion kit (EpCAM- / stromal pools) and then stained with either a oligonucleotide conjugated antibody to B2M/Cd298 (biolegend) or in EpCAM+ cells an additional EpCAM+ oligo conjugated antibody. In the case of the first pool 3 samples were digested fully without separation. All these libraries are 3 prime, scRNA seq processed using 10x v3 single cell RNA seq protocol and co-ordinate with a HTO library (in the case of single stain) or a HTO and ADT library (in EpCAM+ cells case) for demultiplexing RNA libraries were processed as per 10x v3 single cell RNA seq protocol library preparation for illumina i7 sample indexes (CG000183, Rev C), HTO and ADT libraries were extracted from the protocol during SPRI clean up and amplified with custom tru-seq primers as per description in published protocol: https://doi.org/10.1186/s13059-018-1603-1