title: GSM6204015: PCSK9 KO OvxP 2; Mus musculus; RNA-Seq
library_construction_protocol: Liver pieces (~30 mg; 3 per genotype/condition) were homogenized (Polytron) in 1 mL of TriZol on ice. After addition of 0.2 mL of chloroform, and centrifugation, the upper phase was collected. Contaminating DNA was digested with DNAse I and RNA was purified on Zymo-Spin IIICG columns (ZymoResearch, Irvin, CA). RNA libraries were prepared for sequencing using standard Illumina protocols