title: RNA-Seq of heat stressed trinucleate pollen from brassica napus
design: BGISEQ-500 platform for PE100 strand-specific mRNA sequencing with an expected output of 30 million raw reads per sample. After sequencing, the raw reads were filtered. Data filtering included removing adaptor sequences, contamination, and low-quality reads from raw reads. The trinucleate pollen sample collected from plants grown heat-stressed at 40 degrees Celcius for 30mins was used for RNA isolation.