title: GSM6203864: Cerebellum, LPS male; Macaca mulatta; RNA-Seq
library_construction_protocol: Flash frozen cerebellar hemispheres from 2 rhesus macaque fetuses per group were homogenized and lysed with Triton X-100in RNase-free water for nuclei isolation. Isolated nuclei were purified, centrifuged, and resuspended in PBS with RNase inhibitor, and diluted to 700 nuclei/µl for standardized 10x capture and library preparation protocol using 10x Genomics Chromium Next GEM 3' Single Cell Reagent kits v3.1 (10x Genomics, Pleasanton, CA). Library was performed according to the manufacter's instructions. The libraries were sequenced with a Illumina NovaSeq 6000 (Illumina, San Diego, CA). Raw sequencing files were processed with CellRanger 5.0 (10x Genomics) and mapped to the rhesus macaque genome (Mmul_8.0.1) with the option include introns and generated count matrices. snRNA-seq