title: PA gnotobiotic midgut 2
design: Triplicates of RNA per tissue were obtained. Poly(A) mRNA were filtered using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufactures instruction (NEB). cDNA libraries were generated with the Ultra II directional RNA library kit (NEB). All samples were sequenced using an Illumina HiSeq 4000 sequencer (Illumina) with an average of 20 million pair-end reads (2x150 bp) at The James Cancer Center sequencing facilities (The Ohio State University, Columbus OH USA).