title: GSM6542470: 24746_polyA-RNA_oligo-dT_25C_4,SU,treatment_spt20D_BY4741; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were labeled with 4-SU for 6 minutes. Nascent RNA was biotin labeled and purified streptavidin beads. PolyA enriched species were obtained using NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490S, Lot:10043249) and were used for library construction (NEBNext, #E7760S, Lot:10046628).. RS RNA libraries were sequenced in a paired-end mode with NextSeq 500 (Read_1 40 cycles, Read_2 36 cycles).