title: GSM6012534: K562, untransfected; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells from studied experimental conditions were cross-linked with 1% formaldehyde. After quencing and cell lysis, chromatin was sonicated with a LABSONIC M sonicator (Sartorius) to generate 150-300bp DNA fragments. Library preparation from ChIP-isolated DNA was performed using the Ion Xpress™ Plus Fragment Kit and the Ion Xpress™ Barcode Adapters 1-16 Kit (both from ThermoFischer). The protocol for 200bp read-length libraries and for 50-100ng input DNA was employed with some modifications. Due to the small amount of input DNA (10ng) and to avoid adapter concatamerization 1μl of P1 adapter and IonXpress™ Barcodes was used instead of 2μl. Adapter ligation and dual bead size selection for 200bp read length fragments was performed using AMPure XP beads. The size selected libraries were then amplified for 16 cycles.