title: GSM5662157: Follicle_13_post_hCG_1hr; Mus musculus; RNA-Seq
library_construction_protocol: Purify RNA from snap-frozen samples using RNA-SPRI beads (doi: 10.1002/0471142727.mb0422s107) Smart-Seq2 cDNA synthesis was performed on isolated follicle RNA with Maxima H Minus reverse transcriptase (ThermoFisher). Whole transcriptome amplified (WTA) product was purified with AMPure XP beads (Beckman Coulter), quantified with Quanit-iT PicoGreen dsDNA Assay (ThermoFisher), normalized and used for preparing paired-end sequencing libraries with Nextera XT (Illumina, #FC-131) according to the manufacturer's instructions.