title: GSM6012506: CIS_KO.24h_TGFB1.R2; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions from indicated organs were prepared; 106 NK cells per replicate or culture condition were prepared for RNA sequencing. RNA was extracted from sorted ex vivo NK cells using the RNeasy Plus Mini Kit (#74134, QIAGEN, Hilden, Germany), according to the manufacturer's instructions. Purified RNA was measured using an Agilent 2200 TapeStation System (Agilent) with High Sensitivity (HS) RNA ScreenTapes (#5067-5579, Agilent). Next-generation sequencing libraries were generated from 100 ng of high-quality RNA samples with clear 18S and 28S peaks and RNA Integrity Number values ³ 9, using the NEBNext Ultra II Directional RNA Library Prep Kit (#E7760L, New England Biolabs) for Illumina, according to the manufacturer's instructions.