title: Illumina-based WGS of Peperomia dahlstedtii: leaf tissue
design: Libraries were prepared using the Illumina TruSeq Stranded Library Preparation Kit with IDT for Illumina Unique Dual Index adapters following manufacturer's recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts and the pool quantified using the Kapa Biosystems Illumina Library Quantification qPCR kit. The pool was loaded onto one (1) lane of an Illumina HiSeq 4000 flow cell and sequencing was performed in a 2x150bp single read format using HiSeq 4000 SBS reagents. Base calling was done by Illumina Real Time Analysis (RTA) v2.7.7 and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.19.1.