title: GSM5538479: P5A2_25; Pseudomonas aeruginosa; RNA-Seq
library_construction_protocol: Two 200 µl cultures were pooled together and the total RNA was isolated from three samples using an RNeasy Mini Kit (Qiagen) following manufacturer's guidelines. The rRNA was removed using a Ribo-ZeroTM rRNA Removal kit for Bacteria (Epicentre). The first strand cDNA was constructed using the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific), while the second strand was constructed using random hexamers (Thermo Fisher Scientific) and HiFi HotStart Readymix (Kapa Biosystems). The sequencing library was prepared using the Nextera XT (Illumina) tagmentation reaction with 1 ng of RNA serving as an input, following manufacturer's instructions.