title: GSM5259630: Setd2SRI/wt male; Mus musculus; RNA-Seq
library_construction_protocol: Brains were flash frozen in liquid nitrogen, pulverized, and homogenized.  Total RNA was isolated with Trizol (Ambion) and treated with DNase to remove the genomic DNA.  Extracted RNA samples underwent quality control assessment using the RNA tape on Tapestation 4200 (Agilent) and were quantified with Qubit Fluorometer (Thermo Fisher). RNA libraries were prepared with QIAseq Stranded Total RNA library Kit (Qiagen) using 100 ng input RNA.  Ribosomal RNA was depleted with QIAseq FastSelect HMR kit (Qiagen).  RNA was fragmented, reverse transcribed into cDNA, and ligated with Illumina sequencing adaptors at the 5' and 3' ends.  The size selection for libraries was performed using SPRIselect beads (Beckman Coulter) and purity of the libraries was analyzed using the High Sensitivity DNA 1000 tape on the Tapestation 4200 (Agilent) with a size of 300 bps.  The prepared libraries were pooled and sequenced using NextSeq 500 (Illumina); generating ~20 million 2×76 bp paired-end reads per samples.