title: GSM5538790: CD8 NELFB ChIP-seq rep2; Mus musculus; ChIP-Seq
library_construction_protocol: For RNA-seq, RNA was harvested using Trizol reagent. For ChIP-seq, lysates were clarified from sonicated nuclei and Protein-DNA complexes were isolated with antibody. For ATAC-seq, cell nuclei were isolated to perform Tn5 tagmentation to make libraries. For scRNA-seq, cells were loaded onto a Chromium Next GEM Chip G (10x Genomics, PN-1000127) and processed with the Chromium Controller (10x Genomics). RNA libraries were prepared following the Illumina TruSeq stranded mRNA-seq sample preparation guide. ChIP-seq lirabries were prepared using SwiftBiosciences Accel-NGS 2S Plus DNA Library Kit (SwiftBiosciences, 21024). ATAC-seq libraries were prepared by PCR amplification and DNA purification. scRNA-seq libraries were prepared by Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics, PN-1000128).