title: RNA-Seq of ECs treated with Vitexin and subjected to PS exposure.
design: HUVECs pre-incubated with vitexin for 24 hours were subjected to PS for 6 hours. Total RNA from the cells was extracted using the RNeasy kit with DNase treatment (Qiagen) and RNA integrity (RIN > 9) was checked by a Bioanalyzer 2100 system (Agilent Technologies). cDNA libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit according to the manufacturers instructions (Illumina). Cluster was generated using cBot Cluster Generation System with TruSeq PE Cluster Kit v3-cBot-HS (Illumia). After cluster generation, sequencing was performed on an Illumina Novaseq platform and 150 bp paired-end reads were generated. Index of the reference genome was built using Hisat2 v2.0.5 and paired-end clean reads were aligned to the human genome using Hisat2 v2.0.5. FeatureCounts v1.5.0-p3 was used to count the reads numbers mapped to each gene. FPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene.