title: GSM5970808: COPD-3, monocyte; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted with RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. After quality control on an Agilent TapeStation, total RNA with RNA integrity number of 7.0-9.3 was subjected to amplification with the SMART-seq v4 Ultra Low Input RNA Kit for Sequencing (CloneTech). cDNA library was prepared with the Nextera XT DNA Library Prep Kit (illumina). Samples were analyzed on NovaSeq 6000 (illumina) in 2 × 150-bp paired-end mode, which generated 40 million paired reads per sample.