title: RLL-Y2H sequence data of Capra hircus abomasum and Haemonchus contortus
design: Plate onto SD/-AHLT selective plates and grow for 7 days. All positive colonies from SD/-AHLT to isolate the integrated plasmids using the Yeast Plasmid Kit (OMEGA) according to the user manual. Amplify the recombined bait and prey fragments using the isolated integrated plasmids from the pooled positive colonies. Digest the purified PCR products with MmeI. Add MGI adapters to purified 110bp fragments by T4 ligation at 16  for more than 2 h. Amplify the ligation products using primers to construct a high-throughput sequencing library.