title: GSM5027342: T1; Homo sapiens; RNA-Seq
library_construction_protocol: We isolated 1×106 PBMCs/mL peripheral blood. Total ribonucleic acid (RNA) was extracted from 3×106 isolated PBMCs using TRIzol reagent (Thermo Fisher Scientific, No. 15596026) per manufacturer's protocol. A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. MRNA was purified from total RNA using poly-T oligo-attached magnetic beads.