title: GSM6204201: IMQ skin Fb, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Fresh skin was placed in saline at 4 °C until further processing. The epidermis was separated from the dermis by Dispase II (Roche, cat: 04942078001) digestion overnight at 4 °C. Single-cell suspensions were generated by enzyme digestion and subjected to fluorescence-activated cell sorting (FACS) to exclude doublets, debris, and DAPI+ dead cells, and dermal CD45-tdTomato+ live cells were sorted from untreated or 7-day-IMQ-induced PdgfraDreER-tdTomato mouse skin for scRNA-seq. Libraries were generated using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.