title: 16S of preterm infant stool
design: Bioline ISOLATE Fecal DNA Kit for extracion and Illumina metagenomics library preparation protocol was used for library preparation with the Index Kit V2 C and Platinum SuperFi PCR Master Mix . MiSeq Reagent Kit V3 500 cycle for sequencing, targeting the V3 and V4 regions with 785F/800R primers.