title: Flaveria linearis highlight  treated rep1
design: RNA-seq data of Flin were obtained from plant grown under low CO2 (100 ppm) vs normal CO2 (380 ppm) for two weeks and four weeks respectively, and plant grown under high light (with PPFD of 1400 mol m-2 s-1) vs control light condition (500 mol m-2 s-1) were sequenced independently. For RNA extraction, the young fully expanded leaf usually situated on the 2nd or 3rd pair of leaves counting started from the top was used. The chosen leaves were cut and immediately frozen into liquid nitrogen and stored thereafter at -80 oC until further processing. Total RNA was then isolated following the protocol of the PureLInkTM RNA kit (ThermoFisher Scientific, USA). The RNA-sequencing was performed in the Illumina platform in the paired-end mode with a read length of 150 bp.