title: GSM5508885: Bovine mammary epithelial cells induced with LPS at 6 hpi [L_6h_1]; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was extracted from bovine mammary epithelial cells induced with LPS at 0, 6, and 12 hpi using TRizol reagent (Takara Biomedical Technology Co., Ltd, Beijing, China) according to the manufacturer's instructions. Ribosomal RNA was depleted with a Ribo-Zero rRNA removal kit (Epicenter, Madison, WI, USA) before constructing RNA-seq libraries. Preparation of the RNA sequencing library construction was prepared according to the product instruction, and then the library concentration was preliminarily tested using Qubit 2.0, and was adjusted to 1.0 ng/μL.