title: GSM5815716: ATAC_MM1S_MONO3; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were washed with cold PBS and pelleted by centrifugation (500g, 5min, 4°C). Nuclei were isolated by subjecting cells to 100uL of RSB-lysis buffer (10mM Tris-HCl ph 7.4, 10mM NaCl, 3mM MgCl2, 1uL/mL of Digitonin, 1uL/mL of Tween20, and 1uL/mL of NP40) for 3-5 min on ice.  1mL RSB buffer (10mM Tris-HCl ph 7.4, 10mM NaCl, 3mM MgCl2, and 1uL/mL of Tween20) was added to neutralize RSB-lysis buffer. Cells were pelleted by centrifugation (500g, 10min, 4°C) and supernatant was removed. Cells were resuspended in 50uL Transposase mix (25 uL of 2x TD buffer, 2.5 ul of transposase, 16.5 ul of PBS, 0.5 ul of digitonin, 0.5 ul Tween 20, and 5 uL of nuclease free water). The samples were incubated on a thermomixer at 37°C, 1000rpm for 30min. Samples were cleaned up using Zymo DNA Clean & Concentrator-5 kit (D4014) according to manufacturer's instruction in 21uL water. Libraries were prepared with approximately 5x10^4 sorted cells using a Tagment DNA Enzyme and Buffer Large Kit (Illumina, 20034198) and following the Omni-ATAC protocol (PMID: 28846090).