title: GSM6436742: eNPCs, BCL7A KO, 48h differentiation [Sample15_7803_ko_Dif2]; Mus musculus; RNA-Seq
library_construction_protocol: At the indicated time points, cells were scraped, collected and pelleted in PBS. Cell pellets of approximately 500,000 cells were then lysed in Trizol, snap-frozen and stored at -80 °C until use RNA was quantified via HS RNA assay on a Tapestation4200 instrument (Agilent). Approximately 100 ng of extracted ribosomally bound RNA was used as an input for RNA-Seq library preparation according to the TruSeq Stranded mRNA kit (Illumina) following manufacturer's instructions. NGS libraries were quantified via Qubit HS dsDNA assay (Invitrogen) and library size distribution was determined using a HS D1000 assay on a Tapestation4200 instrument (Agilent). Libraries were equimolarly pooled, clustered at 1.8 pM concentration and sequenced SR 75 cycles using High Output v2 chemistry on a NextSeq500 instrument (Illumina).