title: GSM5259646: Control male 1 & 2, pooled, input; Mus musculus; ChIP-Seq
library_construction_protocol: Brains were flash frozen in liquid nitrogen, pulverized, crosslinked in PBS with 1 % formaldehyde, and followed by quenching with 125 mM glycine.  Chromatin was sonicated with bioruptor (Diagenode) to 150-300 bp and immunoprecipitated using anti-H3K36me3 antibody (Abcam, ab9050). DNA libraries were prepared with the QIAseq FX DNA Library Kit (Qiagen) and sequenced using NextSeq 500 Illumina generating ~40 million 76 bp single end reads per samples. ChIP-Seq (All-enzymatic whole genome and hybrid capture library preparation (Qiagen))