title: GSM4808881: Retina P0 Zfp36l1/2 F/F rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Neural retinas from different devlopmental stages were dissected out in cold PBS, soaked in RNAlater, and preserved at -80 degree. Total RNA was isolated/purified using the miRNeasy Mini Kit (Qiagen, 217004) along with on-column digestion of DNA with RNase-Free DNase Set (Qiagen, 79254) following the manufacturers' instructions. RNA quality and quantity were assessed by a BioAnalyzer (Agilent, G2940CA). The samples were then used to generate sequencing libraries with TruSeq RNA Sample Prep Kit v2 kit (Illumina, RS-122-2001) and paired-end sequenced on an Illumina HiSeq 2500 sequencer.