title: GSM5970496: patient 4 post-treatment 1; Homo sapiens; RNA-Seq
library_construction_protocol: scRNASeq was carried out on viable frozen ficoll enriched myeloblast samples from matched patient samples before and during gilteritinib treatment.   Cells were thawed, washed with PBS and dead cells were removed using Dead Cell Removal Kit (Miletnyi).  Cells were then washed twice using scRNASeq Buffer (PBS + 0.04% BSA) to remove any Ca2+ and resuspended at a concentration of 1x106/mL.  Approximately 6000 cells per lane were loaded and processed for cDNA according to the 10X Genomics Chromium Single Cell 3' Reagent Kit v.3.1 in the OSUCCC Genomics Shared Resource.  Samples with less than 10% myeloblasts, estimated by diagnostic pathology report, were run in duplicate or triplicate. Libraries were constructed using the 10X Genomics Chromium Single Cell 3' Reagent Kit v.3.1 and according to the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 using 300 cycles with paired 150bp reads.  A minimum of 20,000 read pairs per cell were obtained.