title: GSM5415390: Con_Hypoxia small RNA #1; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA-seq : Total RNA was isolated from cells using Trizol reagent and the quality of the RNA was confirmed by agarose gel electrophoresis. RNA-seq : TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. The sequencing library was prepared using the IlluminaTruSeq Stranded mRNA LT Sample Prep Kit (Illumina). RNA-seq : The mRNA derived from total RNA using poly-T oligo-attached magnetic beads was fragmented and converted into cDNA. Adapters were ligated to the cDNA and the fragments were amplified by PCR.