title: GSM5259634: H3K9me3_+Dox rep 2; Homo sapiens; ChIP-Seq
library_construction_protocol: T47D-MTVL cells were fixed using 1% formaldehyde, chromatin was extracted and sonicated using a Diagenode Bioruptor to generate fragments between 200 and 500 bp. 30 µg of chromatin was immunoprecipitated overnight using the indicated antibody. Rabbit IgG (Milipore 12-370) was used as a nonspecific control. Input was prepared with 10% of the chromatin material used for an immunoprecipitation. Immunocomplexes were recovered using Protein A magnetic beads from Millipore. Beads with bound antibody/protein/DNA complexes were washed, decross-linked at 65ºC overnight and immunoprecipitated DNA was recovered using the IPure Kit from Diagenode. Library construction and sequencing were performed under BGI (Beijing Genomic Institution) ChIP-seq protocols, using combinatorial Probe-Anchor Synthesis (cPAS) and DNA nanoball sequencing.