title: GSM6143169: 8_1D_UcNK_A256_F4_S256 : SS2 sample; Mus musculus; RNA-Seq
library_construction_protocol: Isolation of cells from the implantation sites were done with enzymatic digestion using Liberase. For SMART-Seq2 processing, single cells were FACS sorted into 96 U-well plates into either trNK (NCR1+CD3ε-TCRb-CD19-CD45+CD122+CD49a+) or cNK (NCR1+CD3ε-TCRb-CD19-CD45+CD122+CD49b+) cells. For droplet-based scRNA-seq, total uNK cells (NCR1+CD3ε-TCRb-CD19-CD45+CD122+) were sorted into Eppendorf tubes. RNA-Seq: SMART-Seq2 scRNA-seq libraries were prepared from trNK and cNK cells from gd9.5 maternal-fetal interface and sorted in 96 U-well plates. Libraries were prepared using Nextera XT DNA library preparation kit and sequenced using a NextSeq 500/550 High Output v2 kit 75 cycles (Illumina) on a Nextseq 500 (Illumina). scRNA-Seq: For droplet-based scRNA-seq, 7,000 cells (sorted uNK or total unsorted cells) were loaded onto the Chromium Single Cell 3' Chip A (10x Genomics) and processed through the chromium controller to generate Gel beads in Emulsion. RNA-seq libraries were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v2 (10x Genomics) as per the manufacturer's instructions. Libraries were sequenced on an Illumina Hi-Seq.