title: GSM5258066: rna_rep04; Chlorocebus aethiops; OTHER
library_construction_protocol: Total RNA was purified with the RNeasy kit at 24 hrs post-transfection 5 ug of total RNA was used to prepare first-strand cDNA using the SuperScript III First-Strand Synthesis kit (Invitrogen) with oligo dT primers.  Spliced transcript was amplified using nested PCR, initially for 6 cycles using the SD6F/SA2R primers, followed by 20 cycles using primers SD2F/jklab0046. Paired-end Illumina sequencing libraries were generated by tailing PCR (6 cycles) with a forward primer within the constant upstream exon and a reverse primer immediately downstream of the N20 barcode. Unique dual i5/i7 indices were added by a second round of tailing PCR (6 cycles), and the resulting products were purified by SPRI bead cleanup.