title: GSM5258430: Week 1 Replicate 5 (1-5); Gallus gallus; RNA-Seq
library_construction_protocol: Myotubes were lysed with TRIzol reagent and chloroform phase separation. RNA (≥ 0.4 μg) was precipitated with isopropyl alcohol, washed with 75% ethanol and resuspended in 10 mM Trizma-HCl (pH = 8.0). RNA libraries were prepared for sequencing using standard Illumina protocols by Novogene Co., Ltd.