title: GSM4805463: WT.T5 scRNA; Mus musculus; RNA-Seq
library_construction_protocol: Cells were captured using the microfluidic devices described for Dropseq following the Online Drop-seq Laboratory Protocol version 3.1 (www.mccarrolllab.com/dropseq). The tagmented and multiplexed cDNA libraries were sequenced in Nextseq 500 using Nextseq500 High Output v.2 kit (75 cycles, Illumina Cat# FC-404-2005) following Macosko et al. recommendations with the following modifications. Drop-seq denatured libraries were loaded at 1.3pM final concentration and were spiked in with 10% of 1.8pM PhiX Control v3 (Illumina Cat# FC-110-3001).