title: GSM6152840: Control_soft palate1; Mus musculus; RNA-Seq
library_construction_protocol: Soft palate tissue (dissected from the posterior third of the palatal region) from E14.5 control and Osr2-Cre;Alk5fl/fl embryos was digested and dissociated into single-cell suspension with TrypLE Express enzyme (12605010, Thermo Fisher Scientific) at 37°C with shaking at 600 rpm for 15 min using a Thermomixer (2231000269, Thermo Fisher Scientific). Single-cell suspension was loaded into the 10X Chromium system and prepared for single-cell library construction using the 10X Genomics Chromium single-cell 3' v2 reagent kit (PN-120267, 10X Genomics) according to the manufacturer's protocol. Sequencing was performed on Novaseq 6000 (Illumina). Library quality control, sequence alignment, and read counts were analyzed using Cell Ranger 4.0.0. Two control samples were combined using the function cellranger aggr so that the combined control sample had comparable cell numbers to the sample from Osr2-Cre;Alk5fl/fl. cDNA libraries were prepared for sequencing using standard 10X Genomics protocols