title: Illumina NovaSeq 6000 paired end sequencing; Single cell RNA-seq of healthy mouse skin fibroblasts
design: Single cell RNA-seq of healthy mouse skin fibroblasts
library_construction_protocol: Skin was minced and digested in enzymatic cocktail for 60 minutes.  Cells were counted and checked for viability using Vi-CELL XR cell counter, and then injected into microfluidic chips to form Gel Beads-in-Emulsion (GEMs) in the 10X Chromium instrument. Reverse transcription was performed on the GEMs, and RT products were purified and amplified. DNA from antibody-derived tags (ADT) was separated from cDNA based on size selection using SPRIselect beads. Cells were processed using Chromium Single Cell Gene Expression 3' v3 Library and Gel Bead Kit following the manufacturer's instructions (10X Genomics, Cat., PN-1000075).