title: GSM6234167: p2_G; Homo sapiens; Hi-C
library_construction_protocol: NK cells were separated from NG-BM and G-BM using FACS 2 million cells per sample were fixed with 1% formaldehyde and suspended in Hi-C lysis buffer, then 100U MobI restriction enzyme (NEB, R0147) was added. After overnight chromatin digestion, biotin was used to fill ends before DNA proximity ligation and crosslink. Then DNA was sheared to 300-500 bp using sonicator. Finally, biotin-labeled DNA was pulled down and libraries were prepared using the NEBNext primer set and sequenced on Illumina HiSeq X Ten.