title: GSM5537807: NT+/+ Fast-2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from intestinal crypts using RNeasy Mini Kit according to manufacturer's protocol. RNA was DNAse-treated and quality analyzed on Agilent Bioanalyzer 2100 RNA Nano chip. RNA-Seq libraries were prepared using KAPA RNA Hyper + RiboErase HMR (Roche). The manufacturer's protocols were used to sequence ribosomal RNA-depleted libraries at 1x100 single-end read on an Illumina HiSeq 2500 in rapid mode, to an average depth of 32 million single-end reads per sample.