title: 16S rRNA seq of effluent treated soil
design: genomic DNA samples were PCR amplified using a universal primer pair 341F and 785R - targeting the V3 and V4 region of the 16S rRNA gene. Amplicons were then purified by gel, end repaired and Illumina specific adapter sequence were ligated to each amplicon. The samples were quantified and individually indexed followed by another purification step. Amplicons were then sequenced on illuminas MiSeq platform, using a MiSeq v3 (600 cycle) kit. 20Mb of data (2x300bp long paired end reads) were produced for each sample.