title: GSM6204117: P09; Homo sapiens; RNA-Seq
library_construction_protocol: Within 4-6 h of tissue acquisition, the specimen was placed in ice-cold RPMI 1640 medium (Gibco, Cat. No. 11875101). Tissue samples were washed in ice-cold phosphate-buffered saline (PBS; Corning, Cat. No. 21- 040-CM) and grossly assessed for adequacy (solid tissue component, free of large hemorrhagic or necrotic areas). Next, the tissue was manually minced with a scalpel blade and filtered through a 40 μm mesh filter (Falcon; Cat. No. 352340) to remove any excess debris or blood. The tissue was then transferred to a sterile gentleMACS C tube (Miltenyi Biotec, Cat. No. 130096334), resuspended in 3-5 mL of Miltenyi Tumor Dissociation Kit buffer (Cat. No. 130-095-929), and mechanically minced to submillimeter particles. The suspension was then digested utilizing the gentleMACS Octo Dissociator (Miltenyi Biotec, Cat. No. 130-095-937) for 25-30 min using the “