title: GSM6234666: LN_CD8_Ramp1 WT..B16_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total CD8+ T-cells were isolated from the spleen of wild-type (CD45.1+) or RAMP1-/- (CD45.2+) mice, expanded and amplified in vitro using a mouse T-cell Activation/Expansion Kit (Miltenyi cat #130-093-627). CD8+ cells from RAMP1-/- and RAMP1wt were injected separately or 1:1 mix through tail vein of Rag1-/- mice. One week after, the mice were inoculated with B16F10-OVA-mCherry cancer cells (5 × 105), and tumor growth was measured daily using a handheld digital caliper. On day 14, tumor and draining lymph node were harvested, and RAMP1-/- (CD45.2+) and RAMP1wt (CD45.1+) CD8+ T-cells were FACS-purified using a FACSAria IIu cell sorter (Becton Dickinson). For FACS-purified cells, RAMP1-/- and RAMP1wt CD8+ T-cells RNA-seq libraries were constructed using KAPA Hyperprep RNA (1x75bp) following the manufacturer's instructions.