title: GSM5750344: Atg16l1 wildtype IFNg treated clone 1 rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Approximately 500,000 AKPS organoid cells were collected per condition and RNA was isolated according to manufacturer's protocol using Qiagen Rneasy Mini Kit 50. Standard input RNA sequencing was performed. Truseq Stranded mRNA kit (Illumina) was used with an input of 100 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on 4200 TapeStation (Agilent Technologies). Libraries were pooled and sequenced on NovaSeq 6000 (Illumina) to generate 30 millions single-end 50-base pair reads for each sample.