title: GSM5639767: AT2aKO 02; Mus musculus; RNA-Seq
library_construction_protocol: AT2 cells were harvested from deeply anesthetized AT2aKO and 2afl/fl control mice as described by Sinha et. al Bio Protoc 6 (2016) with modifications. Lungs were digested in 30U/ml Dispase II in calcium and magnesium-free Hank's balanced salt solution. CD45 –ve cells were first enriched from whole lung cell suspension using magnetic separation on LS Columns using CD45 MicroBeads. AT2 cells were purified using Biotinylated anti-mouse CD326/EpCAM antibody and anti-biotin MicroBeads. 1ug RNA per sample was used for library prepration using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) according to manufacturer's instructions