title: GSM6234408: EG; Ideonella sakaiensis; RNA-Seq
library_construction_protocol: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) with on-column DNA digestion using RNase-Free DNase Set (Qiagen). Bacterial 16S and 23S ribosomal RNAs were removed from total RNA with the Ribo-Zero rRNA removal kit for gram-negative bacteria (Illumina, San Diego, CA). The enriched mRNA fraction was converted to an RNA-Seq library with multiplexing adaptors.