title: GSM6436064: WT bio rep 1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA was extracted from the main inflorescence stems of 2-month-old wild-type Col-0, ccr1-3, fer4, and ccr1-3/fer4 double mutant plants. Total RNA was isolated with Trizol reagent (Invitrogen, USA) and treated with TURBO DNA-free Kit (Ambion) to remove genomic DNA contaminants. RNA was cleaned using an RNA cleanup kit (Qiagen). Purified RNA was quantified using a Nanodrop ND-100 spectrophotometer (NanoDrop Technologies) and evaluated for purity with an Agilent 4200 TapeStation system (Agilent Technologies). Libraries were constructed using a TruSeq stranded mRNAseq prep kit following the standard protocols and run on an Illumina Nextseq 500 platform (2 × 75 bp).