title: GSM5027023: A2780+si34a-1; 1nM; 24h; Homo sapiens; ncRNA-Seq
library_construction_protocol: Cellls were lyzed and proteins were subjected to pull down with a GW182 peptide bound to anti-FLAG Ab beads RNA at a size of 19-35 nt was gel extracted and used to generate library The quality and quantity of the RNA samples was checked using an Agilent bio-analyzer. Small RNA-SEQ libraries were generated using Illumina small RNA SEQ kits using the Illumina provided protocol. Two small RNA-SEQ sub-libraries were generated: one containing library fragments 140-150 bp in size and one containing library fragments 150-200 bp in size (both including the sequencing adator of about 130bp). RNA-Seq; sequenced on an Illumina HiSEQ4000 using Illumina provided reagents and protocols