title: GSM6047153: Neonatal B cells_7h culture_unstimulated_rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with QIAGEN RNeasy Plus mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser. Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Illumina Sequencing (first-strand cDNA synthesis; full-length double strand cDNA amplification by LD-PCR; amplified cDNA purification and validation). The cDNAs were then further prepared using Illumina (San Diego, CA) Nextera DNA Library Preparation Kit (tagmentation; PCR amplification; clean-up and validation).