title: GSM5747758: RNA-seq FL bud E10-E10.25 Hand2_WT replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Samples were defrosted and total RNA extracted using QIAGEN RNeasy kit according to manufactures instructions. The RNA quality was evaluated using the Fragment Analyzer in combination with the High Sensitivity RNA Analysis kit (Advanced Analytical). Only samples with a RIN ≥8.0 were used for library construction. RNA-seq libraries were constructed using the using the Clontech SMARTer kit and 85bp single end reads were sequenced on the Illumina NextSeq 500. 