title: GSM6542226: DC2 Tumor Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA from sorted cells suspended in Trizol was extracted with chloroform. Isopropanol and linear acrylamide were added, and the RNA was precipitated with 75% ethanol. Sample were resuspended in RNase-free water. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 0.387-1ng total RNA with RNA integrity numbers ranging from 3.6 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 0.4-10ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR