title: GSM5747949: HH20; Cavia porcellus; RNA-Seq
library_construction_protocol: Liver sections from the left lateral lobe were snap frozen in liquid nitrogen. 10 mg. liver tissue was purified using the RNeasy Lipid Tissue Mini Kit in accordance with manufacturers instructions. 25 ul of the purified RNA (200-500 ng/ml) was sent to Novogene for sequencing. Total RNA was enriched for mRNA by using oligodT bead system. The mRNA was then fragmented by adding fragmentation buffer, after which the cDNA was synthesized by using mRNA template and random hexamer primers and a custom second strand synthesis buffer (illumina) together with dNATPs, RNase H and DNA polymerase I to initiante second strand synthesis. Secondly after a series of terminal repair, and ligation and sequencing adaptor ligation the double-stranded cDNA library was completed through size selection and PCR enrichment (Novogene).