title: Caulobacter crescentus NA1000; wild-type; mid-log phase; incubated at 10C for 2h; biological replicate C
design: Caulobacter crescentus strain NA1000 was grown aerobically at 30C in minimal medium (M2) until reach mid-log phase (OD 0.5), and then was transferred to an incubator at 10C for 2 hours under continuous agitation. Total RNA was extracted from cultures (2 ml) using Qiagen RNeasy Plus Mini Kit (Qiagen) in accordance with the manufacturer's instruction. The quality of the isolated RNA was checked by running RNA 6000 Pico Kit using Agilent 2100 Bioanalyzer (Agilent). RNA samples were then submitted to the removal of ribosomal RNA and library preparation using Illumina Stranded Total RNA Ligation with RiboZero Plus kit (Illumina). The sizes of cDNA libraries were verified using DNA 1000 Kit (Agilent) and their concentration was measured using Qubit 2.0 (Thermo Fisher), then they were further normalized to 4 uM to assemble the library pool. The samples were sequenced using NextSeq 500/550 Mid Output Kit v2.0 (150 cycles) (Illumina) in the Illumina NextSeq 500 (Illumina) in accordance with the manufacturer's instructions. This is the biological replicate C. One may use the following sequences in order to trim the adapters from FASTQ files: CTGTCTCTTATACACATCT.