title: GSM5258249: ARPE19 cancer cell progession line - ARPE19 rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from ~2 million cells using an RNeasy mini kit (Qiagen 74104). Each replicate was isolated from a freshly thawed cryovial that had been passaged twice prior to extraction. mRNA was prepared for sequencing using the NEB Next Ultra II Directional Library Prep Kit for Illumina (NEB E7760S) and Poly(A) Magnetic Isolation Module (NEB E7490S). Libraries of a median 200 - 400 bp length were generated and sequenced at 2 x 150 bp on a NovaSeq 6000 to a read depth of roughly 50 – 100 million reads.