title: GSM6149721: Wapl delta, rep7 [CT12]; Mus; RNA-Seq
library_construction_protocol: Total RNA was extracted from snap-frozen, whole brain tissue of female, E17.5 embryos using Tri-Pure isolation reagent (Sigma-Aldrich 11667165001) and Qiagen RNeasy Micro kit (Qiagen 74004)with on-column DNase I treatment (Qiagen 74004). Thermo Fisher NANODROP 2000c was used to evaluate purity and yield, and RNAs were stored at -70°C. Samples with RNA integrity numbers > 9.0 as determined using an Agilent BioAnalyzer were purified by oligo-d(T). Libraries were prepared using RNA Sample Prep V2 kit (Illumina) and sequenced on an Illumina HiSeq2500 platform to generate 101 bp paired-end reads.