title: GSM6203779: 48hpf-Control-2; Danio rerio; RNA-Seq
library_construction_protocol: DNase free total RNA was isolated using BioRad Aurum kit following manufacturer's instructions. Total RNA was quantified using Nanodrop spectrophotometer and quality checked using Agilent Technology Tape Station. All the samples had RIN numbers between 9.2-10. RNAsequencing library preparation and sequencing were done at Tufts University Core Facility. Unstranded RNA seq libraries were prepared using TruSeq kit and sequenced on HiSeq 2000 platform.