title: GSM6046911: 8425N.L_H2O_hippocampus; Mus musculus; RNA-Seq
library_construction_protocol: Microglia and astrocytes were respectively isolated using Anti-CD11b and Anti-ACSA-2 MicroBeads (Miltenyi Biotec). Cells were counted and resuspended in 10-15 µL and 20-30 µL of buffer (0.05% BSA in DPBS) for half hippocampus and half cortex respectively, to reach a minimum of 1,000 cells/µL up to around 7,000 cells/µL. Isolated single cells were loaded into the Bio-Rad microfluidic device. Cells were lysed, barcoded, and reverse transcribed inside nanodroplets. Single-cell RNA-seq: SureCell WTA 3_ Library Prep Kit for the ddSEQ System (20014279, Illumina)