title: GSM6365828: SKK-1 AZA treated 1; Homo sapiens; OTHER
library_construction_protocol: gDNA was extracted from >8 x 10^6 cells using standard techniques Libraries were prepared by PCR amplification of shRNA guide strands with custom primers (p7+loop, CAAGCAGAAGACGGCATACGATAGTGAAGCCACAGATGTA; p5+miR3', AATGATACGGCGACCACCGACTAAAGTAGCCCCTTGAATTC) using AmpliTaq Gold polymerase (Thermofisher). PCR products were gel extracted (340bp band) and sequenced using a custom sequencing primer (miR30EcoRISeq, TAGCCCCTTGAATTCCGAGGCAGTAGGCA)