title: GSM5259721: HCT116 - shTGM2-1 -d; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation was performed using RNAeasy isolation kit (Quiagen) according to the manufacturer's instructions. The quality and concentration of libraries were determined by an Agilent 2100 Bioanalyzer and RiboGreen fluorescence on Qubit (Invitrogen). Library preparation was performed at the MPI for Heart and Lung Research (Bad Nauheim) according to protocol The library was prepared using ClonTech's SMARTer Stranded Total RNA Sample Prep Kit and sequenced on an Illumina HiSeq 2000 (San Diego, CA, USA) system using a 100-bp paired-end approach.