title: GSM4809120: CF1903_Rep_1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Animals were pelleted using M9 buffer, subjected to 7 freeze thaw cycles, and RNA was harvested using Trizol method. RNA was then checked for quality and quantity using the Agilent Tapestation and Qubit Fluorometry. RNA libraries were prepared for sequencing using the TruSeq stranded mRNA (PolyA+) kit following standard Illumina protocols