title: WGS of Quercus sp: leaf sample
design: DNA was extracted from dried leaves. Sequencing libraries were generated using NEBNext DNA Library Prep Kit following manufacturer's recommendations and indices were added to each sample. The genomic DNA was randomly fragmented to a size of 350bp by shearing, then DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing. The fragments with adapters were PCR amplified, size selected, and purified (AMPure XP system) and the resulted libraries were analyzed for size distribution by AATI Bioanalyzer and quantified using real-time PCR. The libraries were sequenced on an Illumina NovaSeq 6000 platform with 2 x 150 bp reads. Paired reads were discarded when read contained adapter contamination, when uncertain nucleotides (N) constituted more than 10 percent of either read, and when low quality nucleotides (base quality less than 5) constituted more than 50 percent of either read.