title: GSM5969746: small RNA-seq: A549-AtRDR1 (-)Dox (AGO2-IP); Homo sapiens; RIP-Seq
library_construction_protocol: TRIzol reagent (Invitrogen) was used to isolate total RNA according to the manufacturer's instructions. RNA-seq libraries preparation using the TruSeq Stranded mRNA Sample Prep Kits (Illumina), VAHTS Stranded mRNA-seq Library Prep Kits (Vazyme) and VAHTS Ribo-off Globin & rRNA Depletion Kits(Vazyme). Small RNA-seq libraries preparation using the NEBNext® Small RNA Library Prep Set for Illumina kits. Single-cell RNA-seq libraries preparation using the Chromium Next GEM Single Cell 3' Kit v3.1 (10x Genomics).