title: GSM6046971: oe-YY1 M2 macrophages CM treated LNCaP; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted by the RNA extraction kit (Takara Kusatsu, Shiga, Japan) according to the manufacturer's protocol. 2 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), RiboZero Magnetic Gold Kit (Human/Mouse/Rat) (Epicentre, an Illumina Company) and KAPA Stranded RNA-Seq Library Prep Kit (Illumina) following manufacturer's protocols.