title: GSM5747875: DMSO_Rep1 [EK1]; Mus musculus; RNA-Seq
library_construction_protocol: 16 embryos cells were lysed by freeze-thaw and reverse-transcription reactions were performed using Minus Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA). All samples were pooled together and the cDNA was purified using a purification kit (Zymo Research, Irvine, CA, USA). Full-length cDNA was amplified using the Terra PCR Direct Polymerase Mix buffer (Clontech, Mountain View, CA, USA). Sequencing libraries were constructed using TruePrep DNA Library Prep Kit (Vazyme, Nanjin, China), in accordance with the manufacturer's instructions.