title: GSM5261116: DMSO treated GBM22 orthotopic xenograft (mouse 2 & mouse 3); Homo sapiens; RNA-Seq
library_construction_protocol: Tumor-bearing mice were perfused with PBS, and their brains were removed. Tumor was isolated and dissociated using the Worthington Biochem Papain dissociation kit. Cells were washed two times using ice-cold PBS with 0.1% BSA (w/v), checked for viability using AO/PI dye and a Nexcelom K2 Cellometer, and were loaded into a 10X Chromium chip using 10x Genomics NextGEM 3' chemistry targeting the capture of approximately 10,000 cells per sample. 10X Genomics 3' NextGEM