title: HiSeq X Ten paired end sequencing; 4C-Seq identify the interaction between IRF8 promoter and rs2280381-containing region in human primary monocyte
design: 4C-Seq identify the interaction between IRF8 promoter and rs2280381-containing region in human primary monocyte
library_construction_protocol: human PBMCs are collected and sorted CD14+ monocyte,after neon mediated electro-transfection,total 1x10^7 monocyte cells are collected and fixed by 37% formaldehyde1  DNA was purified using phenol-chloroform and further purified with QIAquick PCR purification kit (28106, Qiagen). The DNA concentration was detected by Qubit (ThermoFisher)