title: GSM5026863: CAST/EiJ resting B cell WGS; Mus musculus; OTHER
library_construction_protocol: Whole genome sequencing: B cells were isolated from spleen of respective mouse strain using EasySep™ Mouse B Cell Isolation Kit (#19854, Stemcell Technologies). Genomic DNA was extracted using DNeasy Blood and tissue kit according to the manufacturer's instructions (#69506, Qiagen). Whole genome sequencing: PCR-free whole genome sequencing library was prepared using TruSeq DNA PCR-Free High Throughput Library Prep Kit (#20015963, Illumina) using 1 ug as input genomic DNA, according to manufacturer's instructions. 100 cycles of sequencing data were acquired on the Illumina NovaSeq 6000 (Illumina).