title: GSM6542056: OC2_R1_oxWGBS; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Tissue blocks of about 30 mg were further dissected out and used for extraction of DNA and total RNA following the manufacturer's instruction of the AllPrep DNA/RNA/miRNA Universal Kit (80224, Qiagen, USA). 1 μg of DNA were used for WGBS-seq and oxWGBS-seq library preparation. Lambda DNA spike-in with artificial methylation and hydroxymethylation modification were using as bisulfite and oxidation control. Individual RNA libraries were pooled and sequenced using paired‑end sequencing with 150 bp reads on an Illumina HiSeq3000 instrument.