title: GSM6047343: Qiagen_S2; Clostridium autoethanogenum; RNA-Seq
library_construction_protocol: Frozen pellets were thawed and total RNA was extracted using cellular lysis in RNase-free acid-washed glass beads (Merck) and RNeasy mini kit (Qiagen) with off-column DNase treatment followed by enrichment and purification using RNA Clean & ConcentratorTM (Zymo Research). Ribosomal RNA were removed as follows: samples referred to as “NuGEN” were processed with Prokaryotic AnyDeplete™ (0363; NuGEN), “Qiagen” samples with QIAseq® FastSelect™ Kit (335925; Qiagen), and “Zymo” samples with Zymo-Seq RiboFree™ Total RNA Library Kit (R3000; Zymo Research) Stranded mRNA libraries were prepared for NuGEN samples with Universal Prokaryotic RNA-Seq, Prokaryotic AnyDeplete™ (0363; NuGEN); for Qiagen samples with QIAseq® Stranded RNA Lib Kit (180743; Qiagen); for Zymo with Zymo-Seq RiboFree™ Total RNA Library Kit (R3000; Zymo Research). Constructed mRNA libraries were prepared for sequencing using the MiSeq v3 150 cycles sequencing kit (MS-102-3001; Illumina).