title: GSM5260007: UF_mouse_3; Ixodes pacificus; RNA-Seq
library_construction_protocol: Prior to extraction, ticks were thoroughly surface sterilized with successive 1 mL washes of 3% hydrogen peroxide, 70% ethanol, and de-ionized H2O to remove surface contaminants. The tick was then lysed and homogenized using the Qiagen TissueLyser II (QIAGEN, Valencia, CA, USA). Tick samples were extracted simultaneously for total DNA and RNA using the Qiagen AllPrep DNA/RNA Micro Kit (QIAGEN, Valencia, CA, USA). We pooled three individual ticks for each experimental replicate.  RNA-seq libraries were prepared from total RNA extracted from ticks followed by rRNA depletion using Depletion of Abundant Sequences by Hybridization (DASH). The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760S) was used to make RNA-seq libraries following the standard manual protocol. The NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760S) was used to make RNA-seq libraries following the standard manual protocol.