title: GSM4809145: Cardiac endothelial cells, het3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from sorted endothelial cells of embryonic hearts were isolated using the RNEasy Micro Kit (Qiagen). Briefly cells were sorted directly to RLT Buffer containing BMeOH and extraction was done following the kit instructions. We also performed the DNAseI on column Kit  (Qiagen) extra steps as recommended. cDNA were amplified with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech-Takara), and libraries then prepared with the Nextera XT DNA library preparation kit (Illumina). Paired end next generation sequencing (Illumina HiSeq 2500)