title: GSM6365510: IMR90, dl1500, replicate 1, RNA-seq mutants; Homo sapiens; RNA-Seq
library_construction_protocol: 24 hours post-infection, cells were lysed with Trizol and total RNA was extracted using the Direct-zol RNA MiniPrep Plus (Zymo research). Eluted RNA was treated with DNase (Invitrogen). Total RNA (1 μg) from dl1500, dl312 and mock infections was processed using a Ribo-Zero rRNA Removal Kit (Epicentre) to remove rRNA. Total RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA library Preparation kit. For the Adenovirus5 mutants experiment, total RNA (1 μg) from wt e1a (dl1500), e1a_RB-b-, e1a_p300-b-, e1a_p400-b- and mock infections was processed using the RiboCop rRNA Depletion Kit (Lexogen), and total RNA-seq libraries were prepared using the Illumina TruSeq stranded mRNA library Preparation kit.