title: 16S V3-V4 Metagenome Analysis of arecant rhizosphere
design: Data quality by fastQC and MultiQC, trimming and processing with Trimgalore, reads imported in to Mothur, alligned to form Contig, checked for high quality, flag contigs with UCHIME algorithm. The filtered contigs were processed and  were binned to Operational taxonomic units (OTUs) at 97% sequence similarity with USEARCH, based on the Silva v.132 database