title: GSM5259636: IR_NestinCFP_Replicate1; Mus musculus; RNA-Seq
library_construction_protocol: mRNA molecules were captured using bead-bound oligo dT(30). Captured mRNA molecules were reverse transcribed with template switching to obtain cDNA. cDNA was amplified by PCR reactions (14 cycles). Aliquots of amplified cDNA from each sample were pooled, and purified with AMPure XP beads. The purified PCR products were used as input for the Nextera XT reactions to generate the sequencing libraries.