title: GSM6046996: mRNA-BisSeq of spleen_WT_rep1; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted by TRIzol® Reagent (Ambion). mRNA was enriched using VAHTS mRNA Capture Beads (Vazyme, #N401). 200 ng mRNA was converted by EZ RNA methylation kit (Zymo Research) according to the manufacturer's protocol with minor modification. More specifically, mRNA was incubated at 70 °C for 10 min, and 60 °C for 1 h. Converted RNA was then reverse transcribed into cDNA using HiScript II Q Select RT SuperMix (Vazyme, #R233-01). VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme)