title: RNA-Seq of discus fish: posterior skin
design: Total RNA was extracted from skin tissue samples using TRIzol Reagent, quality-assesed by Nanodrop2000. Only high-quality total RNA samples ( 5g; 200ng/l; OD260/280=1.8~2.2) were used. mRNA fragments were isolated, fragmented and transcribed into first-strand cDNA using reverse transcriptase and random primers, followed by second strand cDNA synthesis. cDNA fragments were pair-end sequencing using Illumina sequencing system.