title: GSM6012592: Anti-PD-1 Non-responder; Mus musculus; RNA-Seq
library_construction_protocol: Tumor from each group were dissected and processed for single cell dissociation. Duplicates from same group were mixed and sequenced as one sample. Single cell libraries are generated using the 10X Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1.   The resulting libraries are evaluated on D1000 screentape using a TapeStation 4200 (Agilent Technologies), and quantitated using Kapa Biosystems qPCR quantitation kit for Illumina. The resulting pool is then loaded into the appropriate NovaSeq Reagent cartridge and sequenced on a NovaSeq6000 following the manufacturer's recommended protocol (Illumina Inc.)