title: RNA-Seq of Pseudotephritis vau
design: Specimens were extracted with TRIzol reagents (Invitrogen, Grand Island, NY, USA)at the Beijing Genomic Institute (BGI, China). mRNA was selected with Dynabeads mRNA Purification Kit (Invitrogen, Grand Island, NY, USA) and sheared with RNA fragmentationreagents (Invitrogen, Grand Island, NY, USA). cDNA libraries were built with Illumina TruSeq kits and equimolarly pooled with eleven distantly related species for 150bp paired end sequencing. These pools included other flies, beetles and moths that are not part of this study.