title: GSM6149752: MDA-MB-231_NT1_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: MN Nucleospin RNA isolation kit was used according to manufacturer's instructions. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep kit (New England Biolabs). RNA quality was assessed using High Sensitivity RNA Screentape and an Agilent 4200 Tapestation. Single-indexed and multiplexed samples were run on an Illumina Next Seq 500 sequencer using a NextSeq 500 v2 kit (FC-404–2005; Illumina, Can Diago, CA) for paired-end sequencing. Sequencing length was 42 bp.