title: GSM6365793: Group 3 innate lymphoid cell, aged gut ILC3s rep1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Small intestinal ILC3s were sorted from siLP of aged mice. All samples were uniformly resuscitated and washed twice by PBS (with 0.1% BSA), then the cells were 1 ml stain buffer (with 0.1% BSA) and counted. The viability of cells in all samples was assessed >80%. Cells for all samples were labeled with sample tags (BD Mouse Single-Cell Multiplexing Kit, USA), counted, and multiplexed, ready for single-cell capture. Single-cell capture and cDNA synthesis were performed by the BD Rhapsody Single-Cell Analysis System (BD, USA), according to the manufacturer's instructions. scRNA-seq (BD Rhapsody)