title: GSM6203786: foxh1MO_AC_RNAseq_rep1; Xenopus tropicalis; RNA-Seq
library_construction_protocol: For RNA-seq, total RNA from dissected animal cap explants was extracted using Trizol as described (Amin et al., 2014). mRNA was then isolated using NEBNext PolyA mRNA Magnetic Isolation Module (NEB E7490S). Sequencing libraries were prepared using NEBNext Ultra II RNA library prep kit (NEB E7770S)