title: GSM6046957: Immune-enriched cells from 8 mouse tumors, generated by shSCR or shcircCsnk1g3 sarcoma cells [pooled; HTO-lib]; Mus musculus; RNA-Seq
library_construction_protocol: Mouse tumors were digested into single-cell suspensions using the Mouse Tumor Dissociation Kit and gentleMACS dissociator (Miltenyi). CD45+ cells were enriched from each sample with magnetic beads. Enriched samples were individually hashtagged with a unique oligonucleotide barcode (TotalSeq-A, Biolegend) and then pooled together in equal proportions at a concentration of 1000 cells/uL. Single cells were partitioned for RNA capture using 10X Chromium protocols. 10X libraries were prepared from two reactions of the 10X Single Cell 3' Gene Expression kit v3, and sequenced on the NovaSeq 6000 as 800M paired-end reads.