title: GSM5508423: SPT16_AID_V5_Clone1_EtOH_Rep3 [TT-seq]; Mus musculus; OTHER
library_construction_protocol: Medium was aspirated and cells were washed with DPBS. DPBS was aspirated and replaced with fresh medium containing 500 mM 4-thiouridine (4sU). Cells were incubated at 37 degrees Celsius with 5% CO2 for five minutes, then washed with DPBS and trypsinized. Cells were pelleted, washed with DPBS, and RNA was extracted with TRIzol per manufacturer's instructions.