title: ONT-based cDNA sequencing of Peperomia dahlstedtii: mid-stage expanding leaf tissue
design: RNA library preparation was done using the Oxford Nanopore SQK-PCS109 PCR-cDNA Sequencing Kit and PCR Barcoding Kit. Libraries were pooled and loaded onto a GridION FLO-MIN106 flow cell and sequencing performed following manufacturer's recommendations. Total sequencing time was 72 hours. Bases were called using guppy v4.0.11.