title: GSM6203358: MBECsCCM3KO_03; Mus musculus; RNA-Seq
library_construction_protocol: Brain tissue was harvested from animals and either homogenized in TRIzol solution for RNAseq analysis, Brain microvascular endothelial cells were isolated, or Astrocyte tagged ribosomes were isolated as previously described (PMID: 24810037). RNA from brain tissue, brain microvascular endothelial cells, or ribisome purified RNA from Astrocytes was then purified using TRIzol according to the manufacturer's instructions. Following isolation, RNA quality was assessed using an Agilent Tapestation 4200. Only samples with with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the Illumina Stranded mRNA Prep according to the manufacturer's insturctions.