title: GSM5970912: ChIP_seq_PHF10_2; Mus musculus; ChIP-Seq
library_construction_protocol: DNA was eluted by Elution Buffer (50mM Tris-HCl (pH-8,0), SDS 1% and 1mM EDTA) and treated with RNase A for 30 min and protease K (Thermo Fisher Scientific) overnight, incubated at 65 degrees for 6 hours and extracted by phenol-chloroform method For NGS library preparation, 30 ng of DNA and NEBNext Ultra II DNA library prep kit for Illumina was used according to the manufacturer's protocol. Adaptor ligation was performed using NEBNext multiplex oligos for Illumina index primer set #1 (NEB). Quality of DNA was analyzed with Bioanalyzer DNA 1000 Chip kits (Agilent). DNA yields were assessed using Qubit assay kits (Thermo Fisher Scientific)