title: GSM6203243: 1008MM [RNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from approximately 30 mg of frozen LM and matched MM tissues using the RNeasy Fibrous Tissue kit (Cat no: 74704, Qiagen) following the manufacturer's instructions. RNA quality was assessed using the Agilent Bioanalyzer 2100 (Santa Clara, CA). RNA-seq libraries were prepared using the Stranded RNA-seq kit with RiboErase (Cat no: KK8483, KAPA Biosystems, Wilmington, MA). The RNA library concentrations were measured by Qubit TM 4 Fluorometer (Cat no: Q33238, Thermo Fisher Scientific) and library quality was determined using the Agilent Bioanalyzer 2100.