title: GSM6008990: H3K4me3 PLAC-seq on embryonic 12.5 day mouse forebrain, biological replicate 2; Mus musculus; OTHER
library_construction_protocol: In brief, 30-50mg tissues were dissociated, lysed and then digested 2 hours at 37oC using 100 U of MboI followed by biotin fill-in and proximity ligation at room temperature for 4 hours. Then the nuclei were further sonicated and immunoprecipitated against the antibodies. After immunoprecipitation, reverse crosslink was performed overnight at 65 oC after adding proteinase K to extract DNA. DNA fragments containing ligation junctions were enriched with streptavidin beads followed by on-beads end repair, A-tail adding, adapter ligation and PCR amplification.