title: GSM5027034: S4 baseline; Homo sapiens; RNA-Seq
library_construction_protocol: Primary PBMCs were isolated from human blood by density centrifugation using Lymphocyte Separation Medium (MP Biomedicals). RNA was extracted from PBMCs by miRNeasy Micro Kit (Qiagen). RNA was quantified using a Qubit 3.0 fluorometer (Thermo Fisher Scientific) and its integrity confirmed using an Agilent Technologies 2100 Bioanalyzer (Agilent). Dual index libraries were constructed with at least one unique index per each library using TruSeq Stranded Total RNA HT Kit (Illumina), to enable subsequent pooling of equal quantities of individual libraries. The integrity and ratio of pooled libraries was validated using Miseq system (Illumina) and then paired-end sequencing (2×75 bp) was performed on an HiSeq3000 sequencer (Illumina), with the Illumina HiSeq3000 SBS kit.