title: GSM6203858: NOMe-seq, C42B; Homo sapiens; OTHER
library_construction_protocol: The human prostate cancer C42B cells were obtained from ViroMed Laboratories (Minneapolis, MN, USA) Cells were grown at 37 °C in 5% CO2. It was grown in RPMI1640 culture medium, and supplemented with 10% fetal bovine serum (Gibco by Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin and streptomycin. All cell stocks were authenticated at the USC Norris Cancer Center cell culture facility by comparison to the ATCC and/or published genomic criteria for that specific cell line; all cells were documented as free of mycoplasma. First chromatin was treated with M.CviPI methyltransferase to methylate GpC dinucleotides. After purifying M.CviPI-treated DNA, sonication was performed. Bisulfite treatment was then performed to convert all unmethylated Cs to Ts. Libraries were generated using the Accel-NGS Methyl-Seq DNA Library Kit and then sequenced with Illumina HiSeq in paired-end mode