title: GSM5639643: olig2+ cells in zebrafish trunks - 28hpf; Danio rerio; RNA-Seq
library_construction_protocol: Tg(olig2:dsred) trunks were dissected and dissociated with 0.25% trypsin. Fluorescent cells were isolated with FACS. RNA was extracted with Trizol. cDNAs were amplified and libraries were generated with the Single Cell 3' Library and Gel Bead kit V3