title: GSM5538617: P21 auditory nerve WT-3; Mus musculus; RNA-Seq
library_construction_protocol: Microdissections were performed to isolate the auditory nerves from mouse cochleas. Two cochleas from each mouse were pooled for individual samples. Total RNA was purified from each sample using the RNeasy Micro Kit (Qiagen) per manufacturer's instructions. Quality of each total RNA isolation was assessed by TapeStation and samples with low-quality (RIN<7)  were excluded. Libraries were prepared from polyA preps made using 50ng input total RNA and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBNext Poly(A) mRNA Magnetic Isolation Module.