title: GSM5027309: vitro_-GSK3i_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit (QIAGEN) following the manufacturer's instructions. RNA sequencing libraries were constructed using the NEBNext® Ultra RNA Library Prep Kit for Illumina® (NEB England BioLabs). Fragmented and randomly primed 2 × 150 bp paired-end libraries were sequenced using an Illumina HiSeq X Ten system.