title: GSM6436152: 2M, CO, 1; Homo sapiens; RNA-Seq
library_construction_protocol: COs were dissociated into single-cell suspensions using the Papain Dissociation System following manufacturers instructions (Worthington Biochemical, LK003150). 2D cultures were dissociated following standard passaging protocols using Trypsin. Cells were filtered through a 40um strainer and resuspended on ice-cold PBS/2%BSA. For GLICO samples, GFP-positive/DAPInegative spGSCs were sorted by FACS (Becton-Dickinson Aria II) and collected on PBS/2%BSA for Single-cell ATAC-/ RNA-sequencing. For PDX samples, bulk tumor was dissected and dissocated as above. Chromium Single Cell 3′ Library & Gel Bead Kit v.2 (10x Genomics, PN-120237)