title: GSM6588026: FCD IIb sample 13 (gray matter); Homo sapiens; RNA-Seq
library_construction_protocol: Frozen samples were serially cut in cryostat, mounted in PEN membrane covered slides, stained with Cresyl Violet and dehydrated with an ethanol series. The gray (cortical layer) and white matters were mechanically dissected from 3 – 4 sections per sample. Total RNA from each dissected sample was extracted and purified with Trizol. cDNA libraries were produced from 200ng of total RNA using the TruSeq Stranded total RNA kit according to the manufacturer protocol. Sequencing was performed in a HiSeq 2500 platform, in High-Output mode, producing 100-bp paired-end sequences.