title: GSM5415672: m30 WT sh-GLS2 liver; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from all samples using MagMAX-96 for Microarrays Total RNA Isolation Kit (Ambion by Life Technologies) according to manufacturer's specifications. Genomic DNA was removed using MagMAX TURBO DNase Buffer and TURBO DNase from the MagMAX kit listed above. Strand-specific RNA-seq libraries were prepared using KAPA Stranded mRNA-Seq Library Preparation Kit (Kapa Biosystems). Twelve-cycle PCR was performed to amplify libraries.