title: ITS amplicon sequencing of organic forest soils (Quebec, Canada)
design: The ITS gene region was amplified by PCR using the primer pair ITS1F/ITS2R  (White et al., 1990; Gardes and Bruns, 1993). Amplicons were pooled into a single library which was sequenced on a 2 x 250 bps Illumina MiSeq run. Sequences (forward and reverse) were then demultiplexed into fastq files corresponding to each sample.