title: ONT-based WGS of Peperomia dahlstedtii: leaf tissue
design: HMW gDNA libraries were prepared using the Oxford Nanopore SQK-LSK109 Ligation Sequencing Kit. The library was loaded onto a PromethION FLO-PRO002 flow cell and sequencing performed following manufacturer's recommendations. Approximately 20 hours after the start of the run it was paused, the flow cell was nuclease flushed using Nanopore's recommended procedure, a second aliquot of the library was loaded and the run was continued. Total flow cell run time was 72 hours. Bases were called using guppy v4.0.11.