title: GSM5259736: CAD neurite 1; Mus musculus; OTHER
library_construction_protocol: Soma and neurite fractions were collected and RNA isolated using the Zymo Direct-ZOL RNA Miniprep kit followed by cDNA synthesis using Invitrogen SuperScriptIV, both according to the manufacturer's protocol. A first PCR reaction was performed (using Kapa Hifi Ready mix) to specifically amplify reporter cDNA (15=20 cycles). In a second PCR step, Illumina adapters were added (12 cycles).