title: Amplicon bacterial metagenome of Kadokale mangrove sediment sample NEG2_B2
design: Library construction was done with Herculase II Fusion DNA Polymerase Nextera XT Index Kit V2. The template size distribution was verified by running on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip. Prepared libraries were quantified using qPCR according to the Illumina qPCR Quantification Protocol Guide. The libraries were sequenced in paired-end mode with 301 bp read length for both ends, on an Illumina MiSeq platform (Illumina, USA) according to manufacturers protocols.