title: GSM5259606: CL6: Rat liver - normal; Rattus norvegicus; ncRNA-Seq
library_construction_protocol: TRIzol (Invitrogen, Carlsbad, California, USA) was used to extracted total RNA from rat liver tissues, and purified by the manufacturer's instructions.  Utilize NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific, Waltham, Ma, USA) to quantify total RNA and according Agilent 2100 system and RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA) to determine RNA integrity. To achieve accurate lncRNA analysis, the Ribosomal RNA was removed using the Thermo Fisher Scientific Central Ribonucleic Acid Removal Kit. Using next-generation sequencing technology to perform RNA-seq analysis on an Illumina genome analyzer IIx.