title: GSM6204174: scRNAseq_Lineage_Negative_Bone_Marrow_Rit1-M90I_Mx1Cre+; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were stained with Trypan blue and Countess II Automated Cell Counter (ThermoFisher) was used to assess both cell number and viability (90-91%). Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 3-6,700 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 240-370ng of the material was used to prepare sequencing libraries with 10 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 S1 Reagent Kit (100 cycles) (Illumina).  An average of 30 thousand reads was generated per cell.