title: GSM6365625: BK-Bulge-1; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was incubated overnight at 4 °C in 0.25% trypsin/EDTA and the next day at 37 °C for 30 minutes. Single-cell suspensions were then filtered, stained with antibodies and sorted by flow cytometry directly into Trizol (Ambion, 10296028) for RNA extraction. RNA quality was measured by Agilent Bioanalyzer RNA 6000 pico kit (5067-1513) and quantity using nanodrop. Library was synthesized using low input protocol (≤50ng RNA) by SMARTer Stranded Total RNA-Seq Kit v2 – Pico Input Mammalian (Takara) and the library quality was checked by Agilent Bioanalyzer DNA high-sensitivity kit (5067-4626).