title: GSM6234810: RNA-Seq ERY537SClone2_DMSO; Homo sapiens; RNA-Seq
library_construction_protocol: Following inductions cells were washed with PBS and harvested with buffer RLT plus (Qiagen) containing 1% beta-mercaptoethanol (Sigma-Aldrich). Cells were passed through a 21-gauge needle and syringe (Sigma-Aldrich) to lyse genomic DNA before RNA was extracted and purified using a Quick RNA Mini Prep kit (Zymo Research). Using the KAPA Stranded mRNA-Seq kit (KAPA Biosystems), poly(A) selected libraries were created with 500 ng of input RNA per sample.