title: GSM5258192: E2f7/8 DKO tumor 4; Mus musculus; RNA-Seq
library_construction_protocol: Livers and tumors were extracted from the euthanized mouse, cut into small tissue pieces, and immediately snap-frozen in liquid nitrogen. Total RNA was isolated from livers using the RNeasy kit (Qiagen). mRNA was isolated using a poly (A) purist MAG kit (Life Technologies, AM 1922) and purified with mRNA-ONLY™ Eukaryotic mRNA Isolation Kit (Epicentre). Transcriptome libraries were then constructed using SOLiD total RNA-seq kit (Applied Biosystems Life Technologies).