title: GSM6234543: ATAC Isl.12889245 untreat 6h; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were collected from culture and homogenized in permeabilization buffer consisting of 5% BSA, 0.2% IGEPAL-CA630, 1mM DTT, and 1X cOmplete EDTA-free protease inhibitor (Sigma) in 1X PBS. Isolated nuclei were resuspended in 1X TDE1 buffer (Illumina) and quantified using a Countess II Automated Cell Counter (Thermo).  Approximately 50,000 nuclei were tagmented in a 25uL reaction volume using 2.5uL TDE1 (Illumina). Transposition reactions were carried out for 30 minutes at 37C in a thermal cycler. Tagmentation reactions were cleaned up using a 2X reaction volume of Ampure XP beads (Beckman Coulter) and used to prepare libraries using the Nextera XT Dual-Indexed primer system (Nextera) and NEBNext High-Fidelity PCR Master Mix (New England Biolabs). Libraries were sequenced on an Illumina HiSeq 4000 using paired end reads of 100bp.