title: GSM6542460: A009_27-7-2P; Mus musculus; RNA-Seq
library_construction_protocol: Epithelium and mesenchyme of dorsal skins were enzymatically separated using Dispase II (Roche, Tokyo, Japan). Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and dissociated to single cell suspension by TrypLE Select Enzyme (Life Technologies/Gibco). Cell were stained with rat anti-MAdCAM-1 (BD Pharmingen), followed by anti-rat Alexa Fluor 488 - conjugated antibody (Life Technologies). Live placode-enriched (MAdCAM-1 positive) and IFE (MAdCAM-1 negative) cells were sorted on BD Influx Cell Sorter (BD Biosciences) with 100 µm nozzle directly to TRI Reagent® RT – Liquid Samples (Molecular Research Center, Inc. Cincinnati, OH). Total RNA was extracted using Direct-zol RNA Microprep kit (Zymo Research, Irvine, CA), including DNase treatment, according manufacturer's instructions. cDNA libraries were prepared with Ovation SoLo RNA-seq System (NuGen/Tecan Genomics), according to manufacturer's instructions