title: GSM5504851: Male_E11.5_ECKO_Rep_1; Mus musculus; RNA-Seq
library_construction_protocol: Male and female E11.5 and male E13.5 gonads were extracted and digested in 0.05% trypsin. GFP positive mPGCs were sorted into 300 µL RLT buffer and RNA extracted using the Rneasy Micro Kit (Qiagen) Following cleanup, the samples were converted to cDNA following the Ovation RNA-seq System V2 protocol (Nugen). cDNA was indexed using the Ovation Rapid DR Multiplex System (Nugen).