title: Effect of asy-1 mutation on recombination in A. thaliana
design: The generation of the sequencing libraries was performed as previously described (B. A. Rowan, et al., An Ultra High-Density Arabidopsis thaliana Crossover Map That Refines the Influences of Structural Variation and Epigenetic Features. Genetics 213, 771787 (2019)). Pools underwent size selection with magnetic beads, before measuring DNA concentration using a Qubit (Invitrogen) and verifying size distribution with the Agilent Bioanalyzer. Pools were sequenced on an Illumina HiSeq 4000 sequencer, to obtain PE150 reads.