title: GSM6340184: Mutagenesis MPRA in iPSC-CMs day 24 RNA-Rep-3; Homo sapiens; OTHER
library_construction_protocol: Both DNA and RNA were extracted from transfected iPSC-CMs using Quick-DNA/RNA Miniprep Kit (Zymo #D7001). Purified genomic DNA and RNA were stored at -80C. RNA was transcribed into cDNA with a specific primer using the PrimeScript™ RT reagent Kit with gDNA Eraser (Takara #RR047A). Amplicons containing 15 bp barcodes were amplified from gDNA and cDNA.