title: GSM6046946: BMDC, Fucoidan, 24h, rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Trizol reagent (Thermo) and RNeasy kit (Qiagen) and the purified mRNAs were used to synthesize double-stranded cDNAs using SuperScript Double-Stranded cDNA Synthesis Kit (Thermo Fisher Scientific). Sequencing libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina HiSeq 2500.