title: GSM5260597: BRSST+sgEzh2 1% input; Mus musculus; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody and protein A magnetic beads. DNA was eluted from the beads using 0.75% SDS, protease treatment and the Qia MinElute PCR extraction Kit. For library construction the TruSeq ChIP Library Preparation Kit from Illumina was used. Size selection was done via agarose gels.