title: Metagenomics and metatranscriptomics suggest pathways of 3-chloroaniline degradation in wastewater reactors
design: Total RNA extraction methods have been described in Law et al (2016). RNA extractions were performed with Soil/ Fecal RNA Isolation Kit (Zymo Research, Irvine, CA, USA; Catalog# R2040) with some protocol modifications (Law et al, 2016). RNA purity was determined by Thermo Scientific Nanodrop 2000 by calculating an A260/A280 and A260/A230 to screen for contaminants in purified RNA. Extracted RNA concentration was determined by a Qubit 2.0 fluorometer, and RNA integrity (RIN) was measured using the Agilent 2200 Tapestation system, prior to library preparation and sequencing on an Illumina 2500 using a 101 bp paired-end run (Law et al, 2016).