title: GSM5508656: Hela cells transduced with empty vector at time 0h [SA101]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified with TRIZOL (ThermoFisher, #155960026). Total RNA was poly9A)-selected and sequencing libraries were constructed using the TruSeq Stranded mRNA Library Prep (lllumina, # 20020594) and Seq RNA CD Index Plate (Illumina, 20019792). Libraries were sequenced on a NovaSeq 6000 System (Illumina). We multiplex samples in each lane, which yields targeted number of paired-end 100bp reads for each sample.