title: Illumina NovaSeq 6000 paired end sequencing; RNA-Seq of FGF8 treated neural progenitor cells differentiated from human pluripotent stem cells
design: RNA-Seq of FGF8 treated neural progenitor cells differentiated from human pluripotent stem cells
library_construction_protocol: Cells from culture collected using a cell scrapper and pelleted  GnRH differentiation protocol published in 2016. The first twenty days of the protocol  During the stem cell differentiation, first ten days consisted of  dual-SMAD inhibition (Dorsomporphin and SB431542), followed by another ten days of FGF8 treatment.  Using Nucleospin RNA kit from Machery-Nagel. DNase treatment performed seperately using DNase from Promega NEBNext Ultra Directional polyA capture method as paired-end sequencing for read length 150 bp