title: GSM5815305: Lnc152_Overexpression_21_S9_R1_001.fastq.gz; Homo sapiens; RNA-Seq
library_construction_protocol: The frozen xenograft tumor tissues were pulverized using a tissue mill and lysed in Whole Cell Lysis Buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, supplemented with protease and phosphatase inhibitors) for extraction of protein Total RNA from seven xenograft tumor tissue of each group using the RNeasy Plus Kit (Qiagen, 74134) according to the manufacturer's instructions were used to generate strand-specific RNA-seq libraries.  The RNA was then enriched for polyA+ RNA using Dynabeads Oligo(dT)25 (Invitrogen, 61002).  The polyA+ RNA was then used to generate strand-specific RNA-seq libraries as described previously (Zhong et al., 2011).  The RNA-seq libraries were subjected to QC analyses (i.e., number of PCR cycles required to amplify each library, the final library yield, and the size distribution of the final library DNA fragments) and sequenced using an Illumina HiSeq 2500 and NextSeq 500