title: Soil metagenomes
design: The genomic DNA was randomly sheared into short fragments about 350 bp using Covaris. The obtained fragments were end repaired, A-tailed and ligated with Illumina adapter, then PCR amplified, size selected, and purified. The library was checked with Nanodrop 2000 and real-time PCR for quantification and Agilent 2100 for insert size.