title: GSM6436485: Muscle 1 day post NTX [Inflammatory_WT: 1d-4]; Mus musculus; RNA-Seq
library_construction_protocol: Muscle were digested, single cell suspension stained and the different cell populations were sorted as following: FAP as CD45-/CD31-/EGFP+, inflammatory cells as CD45+/CD31-, myogenic progenitors as CD45-/CD31-/a7+/VCAM+, and endothelial cells as CD45-/CD31+ using PDGFRa EGFP wild type B6 background and CCR2KO mouse. Pericytes were sorted as CD45-/CD31-/Sca1-/NG2+ using the NG2 DsRed wild type B6 background mouse. Total tissue was digested from wild type B6 background mouse. RNA extraction was performed with RNAzol based protocol. Sample quality control was performed using the Agilent 2100 Bioanalyzer. Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs). Sequencing was performed on the Illumina NextSeq 500 with Paired End 43bp × 43bp reads.