title: GSM5537507: mixed tissue sample of Eriocheir sinensis; Eriocheir sinensis; RNA-Seq
library_construction_protocol: The total RNA was extracted using the TRIzol method (Invitrogen) for transcriptome analysis. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina Hiseq 2500 platform.