title: GSM5970445: TY4986_Rep_3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Animals were picked as L4's then on Day 1 of adulhood, the animals were collected and washed with M9 three times. The TRIzol was added to the rinsed pellet and stored at -80°C. The samples were then freeze thawed 6 times and the RNA was harvested using the TRIzol method. RNA was checked for quality and concentration using the Agilent Tapestation and Qubit Fluorometry. The library was prepared using the NEBNext Poly(A) mRNA Magnetic Isolation module and NEBNext Multiplex Oligos for Illumina. The DNA libraries were checked for quality and quantified using Tapestation D1000 and Qubit High Sensitivity.