title: GSM5968832: YZ3_ESR1SUZ12E3Y; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and Protein-DNA complexes were isolated with antibody. Samples are sequenced using 100bp paired-end sequencing on an Illumina HiSeq 4000 according to Illumina protocols.Briefly, approximately 5-10ng of DNA is cleaned up using Ampure XP SPRI beads (Beckman Coulter Genomics).  The DNA is then end-repaired, A-tailed, adapter ligated and PCR enriched (8 -10 cycles). Indexed libraries that meet appropriate cut-offs are quantified by both RT-qPCR using a commercially available kit (KAPA Biosystems) and insert size distribution determined with the LabChip GX. Samples with a yield of ≥0.5 ng/µl are used for sequencing.  Flow Cell Preparation and Sequencing: Sample concentrations are normalized to 1 nM and loaded onto an Illumina HiSeq4000 flow cell at a concentration that yields 25 - 50 million passing filter clusters per sample. Samples are sequenced using 100bp paired-end sequencing on an Illumina HiSeq 4000 according to Illumina protocols.