title: RNA-Seq of Prickleless mutant of Solanum viarum Dunal
design: The epidermis of prickleless mutant from 45 days old in vitro grown plantlets was pealed out in liquid nitrogen. Epidermis from five plants were pooled to isolate the RNA and total RNA was isolated using Spectrum Plant Total RNA Kit and treated with the RNase free DNaseI to remove DNA contamination. The quality and quantity of total RNA were analyzed by agarose gel, spectrophotometric analysis and Agilent 2100 Bioanalyzer RNA chip.