title: GSM6047810: TGF_4_hours [scRNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen RNeasy kit. Single-cell suspensions of MCF10A were washed and resuspended in ice-cold PBS containing 0.1% BSA at a concentration of ~2500 cells/µL. Single cells were captured in the ddSEQ microfluidic system (Bio-Rad), mRNA libraries were built using the SureCell WTA 3′ Library Prep Kit (Illumina), libraries sequenced on NextSeq 500 (Illumina) using 150 cycle high output kit (Illumina). Protocols recommended by the manufacturers were followed at each stage.