title: GSM6149749: MDA-MB-231_week4_tumor3; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA (gDNA) from tumor samples was isolated by NucleoSpin Tissue kit (Macherey-Nagel, Germany) according to manufacturer's instructions. Library was constructed with Illumina-compatible adapters. sgRNA cassettes were amplified from genomic DNA using pLentiGuide specific forward and reverse primers in the first PCR. For second PCR, %1-2.5 of the first PCR product was amplified with forward stagger and reverse index primers (specific to sample). Final library fragments of ~350 bp were agarose gel purified. Fragments were quality checked with fragment analyzer and sequenced with Illumina Hiseq (Genewiz, USA)