title: GSM5416018: S5 NMR; Heterocephalus glaber; RNA-Seq
library_construction_protocol: Spleens were dissected and fast frozen on dry-ice. Total RNA was extracted from 3 biological replicates per group using RNeasy extraction kit from Qiagen. RNA was sequenced on the Illumina HiSeq2500 platform according to the manufacturer's instruction at Macrogen (Korea) Illumina TruSeq stranded mRNA LT Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols; library type: Illumina-transcriptome, library: TruSeq stranded mRNA, read length: Illumina 100bp PE