title: GSM5537329: OL_FP_repl2; Mus musculus; OTHER
library_construction_protocol: RNA (total and ribosome footprints) was isolated by phenol chloroform extraction and treated with DNAse I before ribosomal RNA depletion. Total RNA samples were fragmented by alkaline treatment. After PAGE purification, RNA was dephosphorylated and ligated to a preadenylylated linker (Universal miRNA Cloning Linker) before performing reverse transcription with Superscript III (Thermo Fisher Scientific). The cDNAs were precipitated, PAGE purified and circularized with CircLigase. Libraries were obtained by 12 cycles of PCR using primers adapted to Illumina sequencing, and sequenced on an Illumina HiSeq 2500 using single read 50 cycles runs.