title: GSM4808851: FL Late MOLM14 Gilteritinib Day 14 R1 [LIB191203BD_S9_Flt3latGil1D14R1_S55_L002]; Homo sapiens; OTHER
library_construction_protocol: 30 million cells were collected from gilteritinib-treated flasks on day 14 (late resistance screens) and 21 (early resistance screens). DNA was extracted by desalting method 100 micrograms of DNA were used to PCR amplify library using Herculase II enzyme following published protocols and instructions supplied by authors on Addgene  (Shalem, et al Science 2014. 343(6166): p. 84-87, Tzelepis et al, Cell Rep, 2016. 17(4): p. 1193-1205) Shalem et al Science 2014. 343(6166): p. 84-87