title: GSM6204224: Mouse Olfactory Bulb P0; Mus musculus; OTHER
library_construction_protocol: Total RNA preparation was performed usingQIAZOL (Qiagen) and chloroform according tothe protocol from the manufacturer RiboMeth-seq library construction and sequencing were performed as previously described. Triplicate libraries were produced for each cell line or  condition analyzed, and grown to ∼70–80% confluence before collection. A portion of 5 μg of total RNA wasused for input. RNA was partially degraded in alkali at denaturing temperatures. The 20–40-nucleotide fragments were purified by PAGE and linkers addedusing a system relying on a modified Arabidopsis tRNA ligase joining 2′,3′-cyclic phosphateand 5′-phosphate ends. The libraries were sequenced on the Ion Proton platform using Ion PI Chip Kit v.3 (Life Technologies). RiboMeth-seq