title: Ergosterol with Anti-Tumor activity from the Sponge-Derived Fungus Pestalotiopsis sp. XWS03F09
design: B16F10 cells treated with LH-1 for 24 h were sequenced by Shenzhen Huada Ge-nome Co., Ltd. The concentrations of LH-1 was 20 M. Total RNA was extracted from the cells using Trizol (Invitrogen, Carlsbad, CA, USA) according to manual instruction, qualified and quantified using a Nano Drop and Agilent 2100 bioanalyzer (Thermo Fisher Scientific, MA, USA). The sequencing data was filtered with SOAPnuke (v1.5.2). The heatmap was drawn by pheatmap (v1.0.8) according to the gene expression in different samples. Differential expressed genes (DEGs) was identified using DESeq2 package (v1.4.5) under the criterion of the adjusted P < 0.05 and |log2FC| > 1. The cluster-Profiler Bioconductor software was used to enrich gene ontologies (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) (v4.2.2).