title: GSM6234564: snATAC Isl.15314807 untreat 24h; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were collected from culture and homogenized in permeabilization buffer consisting of 5% BSA, 0.2% IGEPAL-CA630, 1mM DTT, and 1X cOmplete EDTA-free protease inhibitor (Sigma) in 1X PBS. Isolated nuclei were resuspended in 1X TDE1 buffer (Illumina) and quantified using a Countess II Automated Cell Counter (Thermo).  Islet nuclei were adjusted to a concentration of approximately 3000 nuclei/uL. We targeted 5000 nuclei per assay for use in the 10X Genomics Chromium Single Cell ATAC assay, v1 chemistry. Sequencing was performed at the UCSD Institute for Genomic Medicine on an Illumina NovaSeq using a specific paired-end 10X ATAC run configuration with reads of 50bp. snATAC-seq