title: GSM5508160: SLE4CD4TRM; Homo sapiens; RNA-Seq
library_construction_protocol: CD4+ Trm cells were sorted into lysis buffer using a BD FACSAria III (BD Biosciences) as much as possible cDNA was generated by using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara),Library construction was performed using the NexteraXT DNA sample preparation kit (Illumina) using half the recommended volumes and reagents. Dual-index, single-read sequencing of pooled libraries was run on a HiSeq2500 sequencer (Illumina) with 58-base reads and a target depth of 5 million reads per sample. Base-calling and demultiplexing were performed automatically on BaseSpace (Illumina) to generate FASTQ files.