title: GSM6340177: WTC-Cas9 iPSC-CMs day17 Rep-6; Homo sapiens; RNA-Seq
library_construction_protocol: iPSC-CMs were lysed in TRIzol reagent (Invitrogen) and total RNA were extracted according to the instructions. Purified RNA was kept at -80C prior to sequencing. RNA samples were analyzed by TapeStation (Agilent) and libraries were generated and sequenced by Genewiz. RNA was processed with poly(A) selection for full length transcripts. Libraries were sequenced on an Illumina HiSeq platform using the 2x150bp configuration with single index.