title: GSM6012716: A172 d9 CD45+HLA-DR+ rep1; Homo sapiens; RNA-Seq
library_construction_protocol: To profile reprogramming-induced transcriptomic changes in human cancer cell lines and human embryonic fibroblasts (HEF), total RNA from 3,000–5,000 FACS-sorted cells was extracted using TRIzol Reagent (ThermoFischer Scientific) following the manufacturer's protocol. cDNA was transcribed with SMARTSeq v4 Ultra low input RNA kit (TakaraBio), amplified with 8 PCR cycles and purified with AMPure XP beads. The cDNA was quantified using High sensitivity DNA kit (Agilent) on Bioanalyzer 2100 (Agilent). Sequencing library was prepared using Nextera XT DNA library preparation kit (Illumina) according to the manufacturer´s instructions. Briefly, cDNA was enzymatically tagmented, followed by indexing via 12 cycles of PCR. The library was normalized with beads, pooled and sequenced on Illumina NextSeq 500 platform (75bp paired-end).