title: GSM5747966: 3 dpa; Danio rerio; RNA-Seq
library_construction_protocol: Fin tissues were collected and immediately frozen at -80C. Nuclei were extracted, fixed in 4% paraformaldehyde, and prepared for sci-RNA-seq3 (please see details at http://atlas.gs.washington.edu/mouse-rna). The library preparation scheme for sci-RNA-seq3 on paraformaldehyde-fixed nuclei was followed, with the following modifications: 2 uL of oligo-dT primers were added to each well with 80,000 nuclei for reverse transcription, the Quick Ligation kit (New England Biolabs) was used in place of T4 ligase, and tagmentation was performed using 1/40th uL per well of i7-loaded TDE1 enzyme (prepared at the University of Washington following published protocols).