title: GSM5662314: SP CD8 Prmt5-CKO Chip H3R8me2s; Mus musculus; ChIP-Seq
library_construction_protocol: Single-cell suspensions were harvested from fresh tissues according to standard procedures and surface-stained in FACS buffer with monoclonal antibodies. Related T cell subsets from mice spleen or thymus were sorted with FACS Aria II Cell Sorter.  For Chip-seq, after the cells were crosslinked and sonicated, the fragmented chromatin fragments were precleared and then immunoprecipitated with Protein A + G magnetic beads coupled with antibodies. For Chip-seq, after reverse crosslinking, ChIP and input DNA fragments were end-repaired and a-tailed using the NEBNext End Repair/dA-Tailing Module (E7442, NEB) followed by adaptor ligation with the NEBNext Ultra Ligation Module (E7445, NEB). The DNA libraries were amplified for 15 cycles and sequenced using Illumina NextSeq 500 in single-end 1x75 sequencing mode.