title: GSM5261140: Activation screen, Library A+B, Bin 7; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Sorted cells were grown overnight and then pelleted. Pellets were treated with lyticase and then plasmid DNA was extracted using a Qiagen Miniprep kit. The region of the plasmid encoding the variable protein fragment was PCR-amplified for 11-20 cycles (based on the OD600 of the culture) using Phusion polymerase with PCR primers that added flanking sequences corresponding to the standard Illumina sequencing primers. After a column cleanup, a second PCR was performed with primers that added sample-specific barcodes and the P5 and P7 sequences for Illumina sequencing. OTHER:sequncing of designed plasmid library