title: GSM5538731: TFD1_S66; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from Bone Marrow Cells harvested from bones of respective genotyped mice, and washed twice with PBS. RNA was collected using Trizol reagent (Catlog#15596026, Ambion), then treated with DNAse using DNAse supplied in TURBO DNA-free™ Kit (Catlog#AM1907, Ambion).The obtained RNA was re-purified using RNeasy kit (Catalog# 74106, Qiagen). RNA was first treated with GlobinClear (Mouse/Rat) to remove any possible globin mRNA, then Illumina TruSeq Stranded TotalRNA Lib Prep Kit. SingleIndex.