title: RNA-seq of biofilm on PS without SMX: P site
design: The V3-V4 hypervariable regions of 16S rRNA were amplified using the primer set 338F (5'-ACTCCTACGGGAGGCAGCA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3')3 combined with barcode and adapter sequences. High throughput 16S rRNA sequencing was performed by the Illumina HiSeq2500 platform for the generation of 2250 bp paired-end reads. Barcode: GGGACGTGTAGACCTA