title: GSM5260025: DMSO; Homo sapiens; OTHER
library_construction_protocol: Nuclei were isolated in a near-physiological isotonic buffer, followed by primary digestion with NlaIII, in situ ligation of digested chromatin, followed by cell lysis and digestion with DpnII. Finally, DNA was circularized by ligation and re-purified. The library was amplified by inverse PCR with viewpoints-specific primers containing a 6-nucleotide long barcode (DMSO: AGAGCTCCTTGCAGCTCTCCAACA; JQ1: GAGCTACCTTGCAGCTCTCCAACA) and susequently coupled to Illumina P5 and P7 adapters for sequencing.