title: GSM5538293: GFP-CTL rep3; Homo sapiens; RNA-Seq
library_construction_protocol: HEK293T were transfected with GFP, GFP-FOXR1 or GFP-M280L mutant using FuGENE6.  Forty-eight hours after transfection, total RNA was purified using the QIAshredder and RNeasy Mini Kit (QIAGEN) and samples were processed with Trizol (Invitrogen). Three biological replicates were processed independently. RNA samples were suspended in DEPC-treated water and concentrations were determined using the Nanodrop ND-1000 (Thermo Scientific) where all samples showed A260/A280 ratios higher than 2.0. RNA integrity was also checked in a bioanalyzer (Agilent 2100). Library preparations and sequencing were performed by The Broad Institute, Cambridge, MA using Illumina HiSeq 2000 technology.