title: RNA-seq of Poecilia reticulata: adult testes
design: Total RNA was isolated using the Direct-zol RNA MicroPrep kit (Zymo Research), following the manufacturers instructions; total RNA concentration was then determined using a Qubit RNA BR (Broad-Range) kit in a Qubit 4.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). RNA quality was assessed using a 4150 TapeStation System (Agilent Technologies, Waldbronn, Germany). Equal amounts of RNA from four testes collected from fish of the same experimental group were pooled; a total of 9 RNA pools were obtained (i.e., three pools/replicates per experimental group). Nine tagged RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA kit.