title: GSM5415634: WT2_S2_L008; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from sciatic nerves using TRIzol (Invitrogen) and the RNeasy Mini Kit (Qiagen, 74106) with on-column DNase treatment. RNA purity was assessed by measuring the A260/A280 ratio using a NanoDrop, and RNA quality checked using an Agilent 2100 Bioanalyzer (Agilent Technologies).  For RNA-sequencing, approximately 150 ng of total RNA per sample was used for library construction and sequenced using the Illumina HiSeq 4000 instrument.