title: GSM5538697: 25_CoH_72_Naive_S77 [PBMC vaccine transcriptome]; Mus musculus; RNA-Seq
library_construction_protocol: SAMPLE GROUP 1: Mouse PBMCs were isolated using FicolÒ-Paque PREMIUM (Millipore Sigma) and RNA was extracted using RNeasy Micro Plus Kit (Qiagen). SAMPLE GROUP 1: The cDNA library was prepared using strand-specific RNA-sequencing protocols. SAMPLE GROUP 1: Samples were run on an Illumina NovaSeq (150 bp paired-end). We obtained an average of 35 million read pairs per sample. SAMPLE GROUP 2: Whole lungs were harvested and RNA was extracted using AllPrep DNA/RNA kit (Qiagen). SAMPLE GROUP 2: The cDNA library was prepared using strand-specific RNA-sequencing protocols. SAMPLE GROUP 2: Samples were run on an Illumina NovaSeq (50 bp paired-end). We obtained an average of 27 million read pairs per sample.