title: GSM5639810: Lin28_SP_cK_P; Mus musculus; RNA-Seq
library_construction_protocol: Briefly, heart samples were mechanically minced into 1-mm3 pieces and digested in collagenase II (150 U mg/mL, Worthington, LS004174) followed by brief low-speed (850 rpm for 2 minutes) centrifugation to remove cardiomyocytes and tissue debris.Lin28S+/- cardiac interstitial cells were obtained by FACS sorting  with Lin28 antibody and concurrent cell surface expression of Lin28/cKit The Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (PN- 1000268), Chromium Single Cell 3' Chip G kit v2 (PN-1000120) and Dual Index Kit TT Set A, (PN-1000215) were used according to the manufacturers instructions in the Chromium Single Cell 3' Reagents Kits V3.1 User Guide.