title: GSM6234289: ChIP-seq input for H3K27ac in control NRVMs; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Cells were washed with PBS and fixed in 1% FA for 10 minutes. Cells were sonicated to give fragments of 200-300bp, then pooled with fixed, sonicated Drosophila S2 cells at a ratio of 4:1 rat:Drosophila, followed by IP. DNA libraries were made using the NEBnext Ultra II DNA library preparation kit for Illumina (Cat no. E7645).