title: GSM5026921: Control 6dpi rep3; Haemaphysalis longicornis; RNA-Seq
library_construction_protocol: Total RNA was extracted from ticks using TRIzol reagent. Enrichment of poly (A)-RNA preparation for RNA Sequencing was performed using NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB) kit. The cDNA libraries were constructed for each pooled RNA sample using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions. The products were purified and enriched by PCR to create the final cDNA libraries and quantified by Agilent2200. The tagged cDNA libraries were pooled in equal ratio and used for 150 bp paired-end sequencing in a single lane of the Illumina HiSeqXTen.