title: GSM5968918: co_int_1; Trichophyton interdigitale; RNA-Seq
library_construction_protocol: ﻿Total RNA was extracted from the tissue using TRIzol® Reagent (Plant RNA Purification Reagent  for plant tissue) according the manufacturer's instructions (Invitrogen) and genomic DNA was  removed using DNase I (TaKara). Then RNA quality was determined by 2100 Bioanalyser  (Agilent) and quantified using the ND-2000 (NanoDrop Technologies). Only high-quality RNA  sample(OD260/280=1.8~2.2, OD260/230≥2.0, RIN≥6.5, 28S:18S≥1.0, >1μg) was used to  construct sequencing library ﻿RNA-seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kit  from Illumina (San Diego, CA) using 1μg of total RNA. Shortly, messenger RNA was isolated  according to polyA selection method by oligo(dT) beads and then fragmented by fragmentation  buffer firstly. Secondly double-stranded cDNA was synthesized using a SuperScript  double-stranded cDNA synthesis kit (Invitrogen, CA) with random hexamer primers (Illumina).﻿Then the synthesized cDNA was subjected to end-repair, phosphorylation and 'A' base addition  according to Illumina's library construction protocol. Libraries were size selected for cDNA target  fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion  DNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, paired-end RNA-seq  sequencing library was sequenced with the Illumina HiSeq xten/NovaSeq 6000 sequencer (2 ×  150bp read length).