title: GSM6436802: db12_gWAT_GSK-LSD1i_RNAseq_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated in Trizol from homogenized tissue and purified using RNeasy columns and Rnase free Dnase digestion according to the manufacturer's instructions (QIAGEN). For mRNA-seq library preparation, total RNA was assessed for quality using an Agilent Tapestation 4200, and samples with an RNA Integrity Number (RIN) greater than 8.0 were used to generate RNA sequencing libraries using the Illumina® Stranded mRNA Prep (Illumina, San Diego, CA). For each sample, 500 ng of RNA was processed following manufacturer's instructions.