title: GSM6047569: prepupal_hemocyte_replicate3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Larval and prepupal were isolated following the protocol published in https://doi.org/10.1007/978-1-4939-7701-7_17. In short, animals of the desired stage were washed and dissected in M3++ medium. Hemocytes were extracted by opening the sample and rinsing out blood cells. GFP positive cells were sorted using the S3e CELL SORTER 488/561-2L4D (Bio-Rad). Total RNA was extracted by Phenol:Chloroform extraction. non stranded mRNA using the TruSeq RS-122-2001 according to manufacturer recommendation