title: GSM6046965: THP-1, H3K27ac, CST; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-Sequencing library preparation was performed according to Illumina's protocol Preparing Samples for ChIP Sequencing of DNA. 10 ng DNA of each sample was converted to phosphorylated blunt-ended; An 'A' base was added to the 3' end of the blunt phosphorylated DNA fragments; Illumina's genomic adapters were ligated to the A tailed DNA fragments; PCR amplification was performed to enrich ligated fragments. The enriched product of ~200-1500 bp was size selected using AMPure XP beads. The library was denatured with 0.1M NaOH to generate single-stranded DNA molecules, and loaded onto channels of the flow cell at 8pM concentration, amplified in situ using Cluster Kit. Sequencing was carried out by running 2×150 cycles on Illumina NovaSeq 6000 according to the manufacturer's instructions.