title: small RNA-Seq rainbow trout blood plasma
design: 50 ng of sncRNA with SMARTer smRNA-Seq Kit (Takara) for Illumina following manufacturer protocol, and libraries size, purity, and concentration were evaluated on a 2100 Bioanalyzer (Agilent technologies). Normalized libraries were pooled and sequenced (single-end, 51 cycles) on a HiSeq2500 (Illumina, San Diego, CA) platform using a HiSeq2500 Rapid Run Sequencing (50SE, 1 lane).