title: RepSeq of human sorted immune subsets
design: Total RNA was isolated using the RNeasy mini kit (Qiagen) according to the manufacturers instructions. In all experiments described here, cDNA libraries were obtained using 5-RACE with unique molecular identifiers (UMI) (20) using Human TCR profiling kit (MiLaboratory LLC). Libraries were sequenced on Illumina NextSeq 500 using 300 cycle reagent kit, paired-end 150 + 150 nt mode. UMI is encoded in read with barcode tggtatcaacgcagagtNNNNtNNNNtNNNN, where N coresponds to UMI themself