title: GSM6351356: WT (N2) L4 stage biological replicate 2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Wash worms off the plate and collect into 1.5mL tubes, wash 3x with H2O, after last wash remove as much water as possible and freeze worm pellet at -80 until needed. Thaw pellet in Qiagen RLT buffer with DTT, agitate worms with narrow tip needle and follow Qiagen RNeasy mini kit instructions for extraction NEBNExt Ultra II Directional RNA library prep kit (oligo dT to enrich for poly(A) mRNA during library prep) stranded, paired end