title: GSM5455280: SUCCS1_siCTRL_96h_HiChIP; Homo sapiens; OTHER
library_construction_protocol: A detailed protocol is outlined in Methods. Briefly, cells were cross-linked with formaldehyde and nuclei washed and collected. Chromatin was digested using the MboI restriction enzyme, end-filled with incorporation of biotin-labelled dATP, subjected to proximity ligation, and proceeded with H3K27ac ChIP. ChIP DNA was purified and concentrated, and biotin pulldown performed with Dynabeads Streptavidin beads followed by magnetic separation. Hi-ChIP sequencing libraries were prepared using the Next Gen DNA Library kit (Cat. no. #53216 and #53264) according to the manufacturer´s instructions, carrying out the reactions on bead-bound DNA followed by magnetic separation. Hi-ChIP library DNA was PCR amplified (8 cycles) and purified using AMPure XP beads.