title: RNA-seq of maize leaf
design: Maize seed Xianyu 335 is a production of DuPont Company (US). The maternal part is PH6WC and the paternal part is PH4CV. Maize seedlings were cultivated in a climate-controlled cultivation chamber, designed and manufactured by the Center of Excellence for Research in Optoelectronic Agriculture at Fujian Agriculture and Forestry University. Uniform blue and red Light-emitting diode (LED) lamps were distributed on the top of each chamber. Maize seeds were cultivated under two light treatments: 660 nm red (R) and 450 nm blue (B). Light density was set at 150 mol m2 s1. 48 seedlings were cultivated in a light chamber for a 24-h photoperiod every day, and each treatment was repeated four times. The temperature and relative humidity of the cultivation environment were set at 25 C and 70 %, respectively. Each fourth leaf bud was collected for subsequent experiments when it began to differentiate.