title: Metagenomics and metatranscriptomics suggest pathways of 3-chloroaniline degradation in wastewater reactors
design: Genomic DNA was extracted as described and sequenced at SCELSE on an Illumina HiSeq platform (Illumina, Inc., San Diego, California, USA). Prior to library preparation, the quality of the DNA samples was assessed on a Bioanalyzer 2100 using a DNA 12000 Chip (Agilent Technologies, Santa Clara, California, USA). Library preparation was performed following Illuminas TruSeq DNA Nano Sample Preparation protocol, starting with 100 ng of genomic DNA and following sizing instructions for 350bp inserts. During adapter ligation, each library was tagged with one of Illuminas TruSeq LT DNA barcodes. Library quantification was performed using Invitrogens Picogreen assay and average library size was determined by running libraries on an Agilent Bioanalyzer DNA 7500 chip. Library concentrations were normalized to 4 nM and validated by qPCR on an Applied Biosystems ViiA-7 real-time thermocycler using qPCR primers recommended in Illuminas qPCR protocol, and Illuminas PhiX control library as standard. Libraries were then pooled and sequenced across two lanes of an Illumina HiSeq 2500 rapid sequencing run at a read-length of 150-bp paired-end.