title: GSM6203261: ChIP_Input_r2; Mus musculus; OTHER
library_construction_protocol: After the appropriate treatment, cells were washed with cold PBS, crosslinked for 5min with 1% Formaldehyde at room temperature and subsequently quenched with 0.1M Glycin (final concentration) for 5min before harvesting. Then, nuclei were isolated and sonicated using the Covaris Chromatin Shearing Kit. ChIP-Immunoprecipation of chromatin was done using antibodies according to the manufacturers recommendation. Libraries were prepared from purified fragments  and sequenced.