title: Nextera Tagmentation of Xenopus borealis: adult female blood cells
design: From a female frog [F2], provided by Jacques Robert at the University of Rochester, DNA was extracted from lysed blood as previously described (10.1038/nature19840) and cleaning with Zymo Genomic DNA Clean & Concentrator kit 2/11/13. This short-insert (325 bp) Nextera tagmentation library weas prepared with Index 1 N712, Index 2 N508 by Jessica Lyons at the University of California Berkeley and sequenced on an Illumina HiSeq 2500 by the Vincent J. Coates Genomics Sequencing Laboratory (VCGSL) at the University of California Berkeley.