title: 16S rRNA amplicons of Fayetteville Green Lake bacteria and archaea
design: Water samples were recovered from Fayetteville Green Lake, NY and size-fractionated by in-line filtration into particle-associated (greater than 2.7 micrometers) and free-living (0.2 micrometers - 2.7 micrometers) assemblages. Filters were immersed in lysis buffer, and immediately frozen on dry ice. DNA was recovered from sample lysates by incubated with agitation using Lysozyme and SDS combined with Proteinase K, sequentially extracted using pH 8-buffered 25:24:1 phenol:chloroform:isomyl alcohol and 24:1 chloroform:isomyl alcohol, concentrated using 100 kDa membranes, and then further concentrated and purified using a Zymo Genomic DNA Clean & Concentrator kit. A260/280 ratios and double-stranded DNA concentrations of the concentrated, purified, DNA samples and negative control were measured using a NanoDrop Lite. The V4-V5 hypervariable regions of the 16S rRNA genes were amplified using the primers 515FY (GTGYCAGCMGCCGCGGTAA) and 926R (CCGYCAATTYMTTTRAGTTT). 350 base-pair paired-end read sequencing of the amplicons were sequenced by the Integrated Microbiome Resource (Dalhousie U., Halifax, Nova Scotia, Canada) using an Illumina MiSeq platform.