title: unenriched RNAseq bosRmut rpoSmut irpoS with IPTG EcAG646 DMC # 1
design: Total RNA was isolated from B. burgdorferi B31 bosR/rpoS double mutant with inducible rpoS cultivated within Dialysis Membrane Chambers implanted into the peritoneal cavities of rats receiving IPTG-treated water. Libraries were prepared using Stranded Total RNA Ligation kit (Illumina), which includes ribodepletion, according to manufacturers instructions. Libraries were run on an Illumina NovaSeq6000.