title: CoA tagSeq
design: Bioreplicate 2 for PM+. TagSeq uses thiol-containing tag RNA to label thiol-containing RNAs, especially CoA-capped RNAs, followed by polyadenylation and isolation of the tagged RNAs and library preparation for Oxford nanopore direct RNA sequencing strategy. NudC- uses reaction buffer to pre-treat RNA sample but NudC was not included while NudC uses NudC to pretreat RNA sample before performing the above tagging reactions and library preparation before sequencing.