title: RNA-seq of rice
design: RNA-sequencing (RNA-seq) was performed by Novogene (Beijing, China). After adapter clipping and quality filter, clean data were remapped to the rice genome sequence (Nipponbare) using Hisat2 software and analyzed with StringTie. DESeq2 software was used to analyze the differentially expressed genes (DEGs), which were defined as those genes with significant expression changes (log2 fold change > 0.5 and padj < 0.05) between ZH11 and OsSPL10 knockout plants.