title: 16S gene sequencing Vitis vinifera: leaves
design: The V5-V6 region of the bacterial 16S rDNA gene was amplified using primers 799F-1115R (Redford et al., 2010; Chelius et al., 2001) to exclude chloroplastic DNA. To avoid a two-stage PCR protocol and reduce PCR biases, each primer contained the Illumina adaptor sequence, a tag and a heterogeneity spacer, as described in Lafort-Lapointe et al., 2017 (799F: 5-CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTxxxxxxxxxxxxHS-AACMGGATTAGATACCCKG-3; 1115R: 5- AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTxxxxxxxxxxxxHS-AGGGTTGCGCTCGTTG-3, where HS represents a 07-base-pair heterogeneity spacer and x a 12 nucleotides tag).