title: GSM5455788: Early2Cell_KO_H1K_4; Mus musculus; RNA-Seq
library_construction_protocol: Zygotes were collected from 6-8-week-old female mice after hormone simulation and mating. The zygotes were cultured in vitro to collect embryos at different developmental stages. M2 eggs were collected from hormone simulated females without mating. M2 eggs or embryos were washed in PBS and transferred into acidic Tyrode's solution to remove zonae pellucidae. Single zona-free eggs or embryos were finally lysed in 2.5 ml RLT plus buffer. G&T-Seq protocol was used to construct single embryo RNA-Seq libraries.