title: GSM6722213: Neutrophils from AT3-gcsf tumor-bearing mice, hashtag library [GR20024-HTO]; Mus musculus; RNA-Seq
library_construction_protocol: Neutrophils were sorted from BM, PB and lung, and then labeled with cell hashing antibodies. Around 24,000 cells (8,000 cells from each hashtagged sample) were loaded into one lane of a 10X Chromium microfluidic chip. Cells capture, lysis and RNA extraction were performed using the 10X V3 Chromium technology following the manufacturer's protocol. Single cell capture, barcoding and library preparation were performed with the 10x Chromium system using version 3 chemistry, following the manufacturer's protocol. cDNA was synthesized and amplified as per manufacturer's protocol and amplified to construct an Illumina sequencing library. cDNA and libraries were checked for quality on an Agilent 4200 TapeStation, quantified by KAPA qPCR.