title: RNA-Seq of L-Soil sample (Lake Hazen)
design: Triplicate ~0.5 g (wet weight) subsamples were taken from sediment and soil, capturing differential runoff regimes. The samples were first washed once with a saline buffer (10 mM EDTA, 50 mM Tris-HCl, 50 mM Na2HPO47H2O at pH 8.0) to remove inhibitors (Poulain et al., 2015; Zhou et al., 1996), and then DNA was extracted from the samples (and a negative reagent control) with the DNeasy PowerSoil Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. The quality of the DNA was checked with a NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE, USA), amplification of the glnA gene from the extracts (and not from negative control) by PCR and gel electrophoresis, and through fluorometric-based methods conducted at Genome Quebec. Library preparation and sequencing was completed as a shotgun metagenome by Genome Quebec (Montreal, QC, Canada) with Illumina HiSeq 2500 PE125 (paired end 2 x 125 bp, insert size approximately 360-420 bp). Libraries were split and run in two lanes for each sample.