title: Nanopore sequencing of Changxianggeng 1813
design: Approximately 10 g of genomic DNA was size-selected (1050 kb) with the BluePippin System (Sage Science, Beverly, MA, USA), and then the DNA was subjected to a 30 L end-repair/dA-tailing reaction using the NEBNext Ultra End Repair/dA-Tailing module (New England Biolabs, MA, USA). The sequencing adaptors were further ligated using the Ligation Sequencing Kit SQK-LSK109 (Oxford Nanopore Technologies, Oxford, UK), based on the manufacturer's instructions. After purifying using Ampure XP beads and ABB wash buffer (Oxford Nanopore Technologies), the resulting library was sequenced on R9.4 flow cells using the PromethION DNA sequencer (Oxford Nanopore Technologies).