title: RNA-Seq  Pharynx primary
design: 1) Isolate the Total RNA from Sample of interest (Cell or Tissue).2) Eliminate DNA contamination using DNase.3) Choose an appropriate kit for library prep process depending on the types of RNA. For mRNA withpoly-A tail, use mRNA purification kit; for non-coding RNAs, such as lincRNA, use ribo-zero RNAremoval Kit to purify RNA of interest.4) Randomly fragment purified RNA for short read sequencing.5) Reverse transcribe fragmented RNA into cDNA.6) Ligate adapters onto both ends of the cDNA fragments.7) After amplifying fragments using PCR, select fragments with insert sizes between 200-400 bp. Forpaired-end sequencing, both ends of the cDNA is sequenced by the read length