title: Effluent treated microcosm soil
design: Uncultured and community DNA from wastewater effluent treated soil samples were fragmented using an enzyme-based approach following part of the protocol from New England BioLabs Next Ultra II kitTM. Resulting fragments were purified (size selected), end-repaired and an Illumina specific adapter sequence was ligated to all fragments. The samples were quantified, individually indexed followed by a second size selection step using AMPure XP Beads. The libraries were quality controlled on a DNA chip (Agilent 2100 Bioanalyzer) and then sequenced on Illuminas MiSeq platform, using a MiSeq v3 (600 cycle) kit according to the manufacturers protocol.