title: GSM5907391: circRNA-seq of  wilms tumor tissue 3; Homo sapiens; ncRNA-Seq
library_construction_protocol: The total RNA samples of 4 pairs of WT samples were treated with the RiboZero   rRNA Removal Kit (Epicentre, WI, USA) for deleting rRNA, according to the   manufacturer's instructions. Next, the rRNA depleted and RNase R digested RNA   samples were fragmented and synthesized cDNA with random primer. Purified the  PCR amplification products of cDNA, then the libraries were quality controlled and   sequenced by NovaSeq6000 (Illumina, San Diego, CA, USA).