title: RNA-Seq of mus musculus: fasted wild-type adult female colon
design: Colonic mucosal scrapings were collected. Total RNA was extracted using TRIzol reagent (Invitrogen Life Technologies, NY, USA) according to the manufacturers instructions. RNA samples were subjected to RNA-seq on an Illumina Novaseq 6000 platform. Clean reads from each sample were aligned to Mus musculus reference genome GRCm38 (mm10) using STAR (Spliced Transcripts Alignment to a Reference) software.