title: GSM5640250: ncSSc_46 [X46]; Homo sapiens; RNA-Seq
library_construction_protocol: Human CD1c+ dendritic cells were purified from heparinised whole blood of SSc patients and matched healthy donors after centrifugation over Ficoll-Paque gradient. Briefly,  CD1c+ dendritic cells were purified from PBMCs using the anti-BDCA1 microbeads (Miltenyi Biotec), on the autoMACs Pro Separator (Miltenyi Biotec) according to manufacturer's protocol. Cells were lysed in RLTplus buffer (Qiagen) containing 1% (v/v) beta-mercaptoethanol (Sigma). Total RNA was purified with the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions. DNAse treatment (RNAse Free DNase I set, Qiagen) on column was performed. RNA-Seq libraries were prepared using the TruSeq Stranded kit (Illumina, San Diego, CA, USA) after poly (A) capture, according to the manufacturer's instructions. Libraries were sequenced on the HiSeq 2000 system (Illumina), using 100bp paired-end reads.