title: GSM6672383: 100nM TBT Day 4 Replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in TriPure (Roche, Basel, Switzerland) and total RNA isolated with MiniPrep columns (Zymo Research, Irvine, CA) Integrity of total RNA was evaluated using Tapestation high-sensitivity RNA screen tapes (Agilent Technologies, Santa Clara, CA). Range of the RNA integrity number (RIN) was 8.0 - 8.6. Strand-specific, barcode-indexed RNA-seq deep sequencing libraries were synthesized from total RNA with ERCC spike-in controls (ThermoFisher Scientific, Waltham, MA) using Ovation RNA-Seq Systems 1-16 for Mouse (NuGen Technologies, San Carlos, CA). Size distribution of the libraries were determined by Tapestation to be 200 – 800 bp, peaking at 300 bp. RNA-seq libraries were quantified using KAPA Illumina library quantification kit (KAPA Biosystems, Wilmington, MA), and up to 12 libraries were pooled in each run of the Illumina NextSeq500 deep sequencer (75 nt + 75 nt, paired-end) to generate fastq raw read sequence files.