title: GSM6722079: HuH-7 cells, si-SLC37A3, #3; Homo sapiens; RNA-Seq
library_construction_protocol: HuH-7 cells were plated in 6-well plates and transfected with si-SLC37A3 and si-NC as previously described. At 48 h after transfection, high quality total RNA was extracted from these cells by TRIzol Reagent. RNA-seq was performed with the assistance of the Applied Protein Technology Co., Ltd. (Shanghai, China). RNA-seq libraries were performed by NEBNext® UltraTM RNA Library Prep Kit for Illumina® (MA, United States) following the manufacturer's instructions.