title: GSM5455538: RNA-seq from Control Testis at P1 replicate 4 [p1_testis_noCre_rep4]; Mus musculus; RNA-Seq
library_construction_protocol: P1/P3 gonads were harvested into Trizol reagent and stored at -80o C.  Total RNA was prepared according to Trizol reagent manufacturer's protocols and further cleaned up using RNAeasy kit protocols and reagents (Qiagen). RNA was quantified via Qubit RNA assay, and 400-500ng total RNA (see sample details) were used in stranded mRNA-seq library preparation (KAPA Biosystems, KK8481) for Illumina sequencing.