title: Metatranscriptomic of Rhizosphere sample, block2, 100 precipitation treatment
design: Total RNA was extracted from 2 g of soil using the RNeasy PowerSoil Total RNA Kit (QIAGEN, Canada) and 0.5 g roots using RNeasy Plant Mini Kit (QIAGEN, Canada). Extracted RNA was treated with DNAse (ThermoFisher, Canada) to remove the DNA prior to sequencing. The absence of DNA was confirmed by the lack of PCR amplification using 16S rRNA gene specific primers. Total RNA was sent for Illumina HiSeq4000 2 x 100 bp pair end sequencing at the Centre dExpertise et de Services Gnome Qubec (Montral, Qubec). Libraries for rhizosphere samples were created using a microbial ribosome subtraction approach to capture all microbial transcripts, whereas libraries for root samples were created using a poly-dT reverse transcription approach to focus on the plant transcripts.