title: circRNA-seq of homo sapiens : adult female endometrium
design: Total RNA was treated with DNase I. Ribosomal RNA and linear RNA was removed using RNase R. Purification was performed using Agencourt RNAClean XP magnetic beads. The circular RNA molecules were fragmented and purified. First-strand and  second-strand cDNA synthesis was performed. End repaired and Y-Adapter ligation to A-tailed cDNA was followed by PCR.