title: GSM6722339: Single granulosa cell (SMART-Seq v4 3' DE Kit) DmGC51_60_S6_IL07; Mus musculus; RNA-Seq
library_construction_protocol: The cDNA amplification using SMART-Seq v4 3'DE Kit was performed according manufacturer's instruction (Takara Bio), except for the step of cell capture.  20 μL of 10×Reaction buffer was prepared (19 μL of 10×Lysis Buffer, 1 μL of RNase Inhibitor), of which 1 μL was mixed with 10.5 μL DDW, resulting in 11.5 μL of cell lysis buffer.  Then, 6.4 μL of cell lysis buffer was added the flat caps of PCR tube, and cells were isolated with LCM into the reaction buffer, followed by the brief centrifugation.  Then, another 5.1 μL of cell lysis buffer was added to the tubes (11.5 μL in total).  The following steps were performed exactly according to the manufacturer's instruction.  Briefly, 1 μ