title: RNA-seq of oncorhynchus kisutch liver (19 months) non-transgenic
design: Pools of two individuals were combined.  RNA was extracted using an RNeasy Mini Kit (Qiagen) from whole liver tissue.  Stranded mRNA libraries were produced at the Centre d'expertise et de services Gnome Qubec.  RNA quality (RIN > 8) was verified on a NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies) and a 2100 Bioanalyzer (Agilent Technologies).  A NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs - NEB) was used for mRNA enrichment from 250 ng of total RNA and cDNA synthesis was performed using the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (NEB).  A NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) was used for the remaining library steps with adapters and primers from NEB.  Library concentrations were quantified using the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems).