title: GSM6674816: 114T; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using AllPrep DNA/RNA FFPE kit® (Qiagen, Hilden, Germany) following the manufacturer's recommendations. RNA quantity and quality were determined with Nanodrop 2000 Spectrophotometer® (ThermoFisher Scientific, Wilmington, USA) and the Agilent RNA 6000 Nano kit® (Agilent Technologies, Santa Clara, CA), respectively. All samples were suitable for library preparation. For library preparation, 1 g of total RNA and the TruSeq Stranded Total RNA Library Prep kit® with Ribo-Zero Human/Mouse/Rat (Illumina, Inc., San Diego, CA, USA) were used. Fragmentation step was omitted in most of the samples due to sample quality, while in five samples it was done according to recommendations from Illumina®. Validation of libraries was performed in the Agilent 2100 Bioanalyzer® system and then normalization to 10 nM was done with the Qubit dsDNA HS Assay kit® (ThermoFisher Scientific, Wilmington, USA), before cluster generation. Sequencing was performed in 12-pooled samples at 1 x 75 bp with single-end strategy, with no sequencing results in six tumor samples.