title: Illumina sequencing of Poeciliopsis prolifica genome: adule male body
design: A 10 Genomics linked-read library was performed on 1.1 ng of genomic DNA from the same male sample, using the Chromium Genome Reagent Kit v2 and Chromium Genome Library & Gel Bead Kit version 2 on a 10 Genomics Chromium Controller (10 Genomics, Inc., San Francisco, CA, USA). The final library concentration was measured on a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The library size distribution was determined by LabChip GX Touch HT Nucleic Acid Analyzer (PerkinElmer, Hopkinton, MA, USA). The libraries were measured by qPCR before being sequenced on an Illumina HiSeq X Ten System with 150-bp paired-end reads at the Genomics Service Laboratory at the HudsonAlpha Institute for Biotechnology (HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA).