title: scRNAseq of human vitiligo lesion
design: This study was approved by the Ethics Committee of Huashan Hospital, Fudan University (KY2020-1137). Four different skin samples were obtained from the same patient, which were: 1) Donor skin: skin sample from the donor site which were used for the generation of skin sheets; 2) Skin sheets: autologous cultured epithelium derived from the donor skin; 3) Lesion skin: skin sample from skin lesions before transplantation; 4) Repigmentation Skin: skin sample from ACEG treated site 1 year after surgery.The single cell suspension was prepared and loaded onto the 10X Chromium Single Cell Platform (10X Genomics) at a concentration of 1000 cells/ L (Single Cell 3 library and Gel Bead Kit v.3) as described in the manufacturers protocol. For each sample, 5000 cells were captured. Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, complementary DNA amplification and library construction were all performed according to the manufacturers protocol. Qubit was used for library quantification before pooling. The final library pool was sequenced on the Illumina Novaseq 6000 instrument using 150-base-paired-end reads. Scrublet (version Python 3.7.3) and Cellranger (4.0.0) were used to generate clean data.