title: GSM5509440: ChAR-Seq_Pol_III_Untreated_rep2; Mus musculus; OTHER
library_construction_protocol: Pol III degron cells were collected and mixed with 10% S2 cells. The chromatin-associated RNA were collected as reported (Dev M. Bhatt et al., 2012) and extracted with Trizol reagents as manufacture instruction. Chromatin-associated RNA were delivered to Novogene Company. The concentration, purity, and integrity of RNA were examined and followed by ribosomal RNA depletion and strand-specific library construction.