title: RNA-Seq of Neocalanus flemingeri pre-adult CV Seward experiment 2019 with No Food E1
design: Total RNA was extracted from individual pre-adults (n=3/treatment) using QIAGEN RNeasy Plus Mini Kit (catalog # 74134) in combination with a Qiashredder column (catalog # 79654) and shipped on dry ice to the Georgia Genomics Bioinformatics Core (https://dna.uga.edu). There double-stranded cDNA libraries were multiplexed and sequenced using Illumina Next-Seq 500 instrument (High Output Flow Cell, 75 bp, paired end).