title: Transcriptome Sequencing of Indian Acute Leukaemia
design: For transcriptome sequencing, total RNA libraries were prepared using Illumina TruSeq RNA Library Prep Kit v2. Briefly, the mRNA was isolated from total RNA using oligo-dT beads (NEB; New England Biolabs, Ipswich, Massachusetts, United States). The isolated mRNA was then fragmented to 200-250bp, followed by double-stranded cDNA synthesis. Adapter ligation was performed after end-repair, and PCR amplification was performed. After constructing the libraries, their concentrations and insert sizes were confirmed using Qubit and Agilent Tapestation, respectively.