title: Time 1,  mutant deleted BSD2
design: The RNA was extracted using MasterPure Yeast RNA Purification Kit (Lucigen). The RNA quality was checked by Agilent RNA 6000 Nano kit on Agilent 2100 Bioanalyzer (Agilent) and RNA quantity was determined by Qubit RNA BR Assay Kit (Thermo Fischer Scientific. The RNA extracted was used to perform the Illumina TruSeq Stranded mRNA Sample Prep (Low-Throughput Protocol). Library was validated by quality control using Agilent DNA 1000 kit on the 2100 Bioanalyzer (Agilent) and DNA quantity was determined by qPCR using KAPA qPCR MasterMix (Roche) on the Viia7 Real-Time PCR System (Thermo Fischer Scientific). DNA template for cluster generation was prepared with the indexed DNA library normalized to 10nM and then pooled. All libraries were performed manually. And all the kits employed following the manufacturers protocols.