title: GSM5640096: VavCre-Jag1 f/f, 1; Mus musculus; RNA-Seq
library_construction_protocol: 2000 Fetal liver HSCs from E14.5 Jag1f/f, VavCre- and Jag1f/f, VavCre+ were sorted into RNA lysis buffer (Invitrogen) and stored at -80ºC until RNA extraction. Total RNA was extracted using PicoPure™ RNA Isolation Kit (ThemoFisher Scientific, USA) with inclusion of DNase treatment step. RNA sequencing libraries for Illumina sequencing were prepared in one batch using SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) along with negative and positive controls.