title: clone A without copper day 3
design: 1 ml from Ambr cell samples were collected centrifuged and re-suspended in 200 l RNAlater Stabilization Solution (Invitrogen, ThemoFisher) to stabilize and preserve the cells.These were then kept at 4C overnight and then stored at -80C. Frozen pellets were thawed and RNA were extracted following the Qiagen RNeasy Plus Universal Mini Kit (Qiagen, Hilden DE)according to manufacturers instructions. Subsequently, extracted RNA quality were analyzed on anAgilent Bioanalyzer 2100 system together with the Agilent RNA 6000 Nano kit (Agilent Technologies,  Santa Clara,  CA, US)and all samples had an RNA Integrity number (RIN) >8. Sequencing was performed on aIllumina HiSeq 2500 High Output Mode, at paired-end 2x150bp(Illumina HiSeq platform via a commercial service of Eurofins MWG GmbH,  Ebersberg, Germany).