title: Plasmid library
design: The barcode region of the muPyV genome was pre-amplified during an enrichment PCR and subsequently a 75 bp fragment encompassing the barcode region was amplified from the enrichment PCR product, using customized staggered indexing primers during an indexing PCR. The indexing PCR amplicon sizes range from 211 to 216bp and contain adapters and i5 and i7 indexes required for Illumina NextSeq SR75 sequencing.