title: Metagenomic sequencing of deep-sea sediments of ferromanganese nodule fields:?sample KW1-S15
design: The DNA sample was fragmented by sonication to a size of 350bp,then DNA fragments were end-polished,A-tailed,and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification.At last,PCR products were purified(AMPure XP system) and libraries were analysed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.