title: GSM6673846: GD15M_LM2_H3K4me3; Sus scrofa; ChIP-Seq
library_construction_protocol: The samples were lysed with precooled lysis buffer at 4°C for 10 min, and resuspended in wash buffer. Then the number of nuclei was counted. Approximately 100,000 nuclei were resuspended in wash buffer, mixed with 10 µL equilibrated Concanavalin A beads. Then the beads were resuspended in a mixture containing diluted primary antibody to obtain the immunoprecipitated DNA. The immunoprecipitated DNA and immunoprecipitated control (IgG) were purified. ChIP-seq libraries were constructed following the manufacturer's instructions for Illumina, and were sequenced on an Illumina NovaSeq PE150 platform (Yingzi gene technology, Wuhan, China).