title: PacBio HiFi whole genome sequencing of: Adult Zeugodacus cucurbitae (ToLID: idZeuCucr1)
design: High molecular weight (HMW) DNA extraction was performed from a whole male adult using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 3 with the Speed 31 protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 3.0 and the library was size-selected with beads to remove library molecules less than 3kb in length to generate the final library for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v3.2, and SMRT Cell 8M. To target HiFi reads, the library was sequenced using a 30 hour movie time on one SMRTcell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule using a 99.5% consensus accuracy cutoff.