title: microbiome of Ulva sensu lato on salinity gradient
design: DNA extracted with the Qiagen PowerSoil kit. Full length 16S rRNA gene amplified with primers 27F and 1492R. Amplicons multiplexed with Oxford Nanopore barcodes (EXP-PBC096) and pooled in equimolar ratios. Final library prepared with the ligation-based sequencing kit SQK-LSK109 (Oxford Nanopore).