title: 16S-seq of Bos taurus feces
design: Composite fecal sample was prepared by mixing ten individual fecal samples (5 g each) in a sterile polyethylene tube. Forty grams (wet weight) of the cattle composite fecal sample were then mixed with 200 mL sterile distilled water in a sterile 250 mL bottle and shaken at 200 rpm for about 30 minutes until no caking solids were present. 300ul was used for DNA extraction and sequencing.