title: small RNA-seq of Notospermus geniculatus: female gonads 2
design: Animals were left in seawater and 7% MgCl2H2O (1:1 ratio) for 15; gonads were then dissected in 7% MgCl2H2O on ice and stored in RNAlater (Thermo Fisher Scientific Inc., Waltham, USA), following manufacturers instructions. Total RNA was extracted using a standard chloforom:TRI Reagent (Merck KGaA, Darmstadt, Germany) protocol, following manufacturers instructions. The TruSeq Small RNA library kit (Illumina, San Diego, USA) was used to prepare six sRNA libraries that were sequenced on an Illumina HiSeq2500 platform. Both library preparation and high-throughput sequencing were carried out at the Macrogen Inc. facility (Seoul, South Korea).