title: Cap-seq of Caenorhabditis elegans: adult hermaphrodite
design: Total RNA was extracted from whole animals of ~100,000 synchronized young adults. Terminator exonuclease (Epicentre) was used to selectively degrade monophosphorylated RNAs. Quick CIP (New England Biolabs) was used to dephosphorylate non-capped triphosphorylated RNAs. RppH was used with Thermopol Buffer (New England Biolabs) to decap RNAs for ligation. Libraries were prepared according to the manufacturers protocol of the NEBNext Multiplex Small RNA Sample Prep Set for Illumina-Library Preparation (New England Biolabs). NEBNext Multiplex Oligos for Illumina Index Primers were used for library preparation (New England Biolabs). Libraries were sequenced using an Illumina NovaSeq6000 to obtain single-end 100 nt sequences at the University of Chicago Genomic Facility.