title: GSM6265770: VCaP Romi; Homo sapiens; ChIP-Seq
library_construction_protocol: The lysates were immunoprecipitation with H2A-K130ac antibodies (2 ug) for 3 hours. Ten nanograms of immunoprecipitated DNA was fragmented to 300 base pairs using a Covaris M220 Focused-ultrasonicator (Covaris, Inc., Woburn, MA) and then used to generate sequencing libraries using the Kapa Hyper Prep Kit (Roche Sequencing Solutions Inc., Pleasanton, CA). The size and quality of the library was evaluated using the Agilent BioAnalyzer (Agilent Technologies, Inc., Santa Clara, CA), and the library was quantitated with the Kapa Library Quantification Kit.  Each enriched DNA library was then sequenced on an Illumina NextSeq 500 sequencer to generate 40-50 million 75-base paired-end reads (Illumina, Inc., San Diego, CA).  The raw sequence data were aligned using BowTie 2 [1], and the binding sites were identified using the MACS peak-finding software