title: GSM5640906: Mo_PBS_2; Aedes aegypti; RNA-Seq
library_construction_protocol: Total RNA from the pool of five female mosquitoes in the control and spinetoram-treated group with and without DENV-2 infection was extracted by using the TRIzol reagent according to the manufacturer's protocol (Invitrogen, California, USA). To construct the library for the MGISEQ-2000, total RNA was used for library construction using the MGIEasy RNA Directional Library Prep Kit (MGI). Next, paired-end sequencing was performed using the MGISEQ-2000 sequencing instrument, according to the manufacturer's instructions, yielding 150-bp paired-end reads. 