title: 16s Metagenome Sequencing and Analysis of Dental Biofilm Samples
design: 8ul  from  each  sample  was  end-repaired,  cleaned  up  with  1x  AmPure  beads.  Native barcode ligation was performed with NEB blunt/ TA ligase using EXP-NBD104 (ONT) and cleaned with 1xAmPure beads. Qubit  quantified  barcode  ligated  DNA  sample  was  Adapter  ligated  for  15  minutes  using NEBNext Quick Ligation Module. Libraries generated were cleaned  up  using  0.6X  AmPure  beads,  eluted  with  15  l  elution buffer and used for sequencing.