title: 16S NGS Microalgae commmunity
design: lllumina sequencing was performed in duplicates on all samples from the second and third sampling campaign for prokaryotic and eukaryotic communities at Stab Vida Lda (Lisbon, Portugal). For bacteria, the V3-V4 hypervariable region on the 16S gene was selected and amplified with the primers Bakt_341F: CCTACGGGNGGCWGCAG and Bakt_805R: GACTACHVGGGTATCTAATCC (Herlemann et al., 2011) while for eukaryotes the 18S V9 region was amplified with the primers 1389F: TTGTACACACCGCCC, 1510R: CCTTCYGCAGGTTCACCTAC was targeted (Amaral-Zettler et al. 2009). The generated DNA libraries were sequenced with MiSeq Reagent Kit Nano in the lllumina MiSeq platform, using 300bp paired-end sequencing reads.