title: GSM7047535: Cold_3h; Manihot esculenta; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and treated with Turbo DNase (ThermoFisher Scientific) according to manufacturers' instructions. DNase-treated RNA was recovered by acid–phenol extraction and ethanol precipitation. Total RNA was subjected to Poly-A enrichment and libraries were prepared for Direct RNA-Seq using SQK-RNA002 kit following manufacturer's protocol.