title: Effect of biochar on antibiotic resistance genes in anaerobic digestion system of antibiotic mycelial dreg:nZVI-BC
design: Aiming at the problem of antibiotics mycelial dreg (AMD) treatment and removal of antibiotic resistance genes (ARGs), this study adopted anaerobic digestion (AD) technology, and added biochar (BC) and biochar loaded with nanosized zero-valent iron (nZVI-BC) to promote the AD of AMD and enhance the removal of ARGs.Therefore, metagenomic sequencing technology was used to detect the changes of microbial community structure and antibiotic resistance genes.