title: GSM5640392: KATO/DDP_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted by Nucleozol Ref 740404.200 and the quality of total RNA was checkedd by nanodrop and agarose gel electrophoresis. Total RNA extracted was checked for quality and then mRNA was enriched from total RNA by using oilgodT beads. Then, mRNA was fragmented and cDNA was synthesized. After first strand synthesis, custom second strand synthesis buffer (illumina) was used to generate the second strand by nick translation. After that it undergoes purification, adapters ligation and PCR enrichment.