title: GSM6724049: M6; Mus musculus; RNA-Seq
library_construction_protocol: After the mice were killed, their liver tissues were removed and the RNA in the tissues was extracted for sequencing by the experimental Trizol method. First, Oligo(dT) magnetic beads were used to separate the mature mRNA from total RNA. Then, the fragmented mRNA was set as template for the synthesis of the first and second strands of cDNA. After being purified, the cDNA was subjected to PCR amplification, and the cDNA library was obtained accordingly. Finally, Illumina Novaseq 6000 was used for RNA sequencing after quality inspection of the obtained library. The statistical power of this experimental design, calculated inRNASeqPower is 0.88, based on an average sequencing depth of 117X, CV of 0.4.