title: Whole Metagenome Sequencing of exclusively breastfed human infant stool
design: DNA was extracted from raw stool samples in cryogenic storage that had sufficient sample volume. Extraction was performed from 0.25g of each stool sample with Power Fecal DNA Isolation Kit (MO BIO) per kit instructions.  DNA concentration was meas-ured using Qbit.  Nextera XT adapters generated an amplified library that was se-quenced to obtain 150bp DNA paired end reads to a depth of 10 million base pairs per sample using Illumina NovaSeq 6000 (Illumina Corp.)