title: RNA-seq of MCF-7
design: RNA was extracted from the samples using the Qiagen miRNeasy mini kit and ribo-depletedwith the Qiagen QIAseq FastSelect rRNA HMR Kit. Size selection of RNA fragments was donewith SPRI Beads-based Size Selection. High quality libraries were prepared using NEBNext UltraII Directional RNA Library Prep Kit according to manufacturers protocols and paired-end readsof 151bp read lenght were generated on the Illumina Novoseq 6000 platform. Transcriptome Analysis