title: ChIP-Seq of Mus Musculus: adult male VSMC
design: 210^6 cells were cross-linked and used for each immunoprecipitation. DNA was sheared to 500-1000 bps by water sonication. Dynabeads Protein G (Thermo Fisher Scientific, Ca# 10007D) was used to pull down the antibody-antigen complexes immunoprecipitated with antibodies anti-H3K79me2 (Abcam Ca# ab3594). Immunoprecipitated DNA was extracted with phenol-chloroform, precipitated with ethanol, and further eluted in 60 ul.  The libraries were generated using Thruplex DNA-seq kit (R400427, Takara Bio). ChIP-seq libraries were obtained from about 10 ng of double-stranded DNA. The workflow consisted of three steps: end repair to generate blunt ends, adaptor ligation with optional multiplexing, and PCR.