title: GSM6153504: CKO female2, Riboseq; Mus musculus; OTHER
library_construction_protocol: RNA-seq: RNA was harvested using the RNAeasy kit (Qiagen, 74034). 50 ng of total RNA was used for the construction of sequencing libraries. Riboseq: Ribosome protected fragments were isolated on a 15% denaturing urea gel followed by gel extraction and Trizol-based RNA isolation. RNAseq: cDNA libraries for RNA-seq were prepared using the Kapa mRNA HyperPrep kit with mRNA capture (KapaBiosystems, KR1352) Riboseq: cDNA libraries for Ribo-seq were prepared using the Qiagen miRNA library prep kit (331502)