title: GSM6722727: WT MCF-7 H3K36me3 espan 1h; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag was performed as previously reported(Kaya-Okur HS et al., 2019) CUT&Tag library PCR amplification was performed with NEBNext 2× PCR Master Mix and standard Illumina Nextera indexing primers. The libraries were purified with Ampure XP beads at a ratio of 1:1. ChIP-seq libraries were prepared from 10 ng EZH2 ChIP, 100 ng H3K36me2 ChIP and input DNA using the KAPA Hyper prep kit (KAPA).