title: Sediment
design: DNA was extracted from the sediment using the E.Z.N.A Mag-Bind Soil DNA Kit extraction kit, and genomic DNA was accurately quantified using the Qubit 3.0 DNA Detection Kit to determine the amount of DNA that should be added to the PCR reaction. The V3-V4 region of the 16S rRNA gene was amplified using primers 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-59).