title: sRNA-seq of cotton:leaves
design: Total RNA was extracted from the leaf samples using a plant RNA mini kit  according to the manufacturers instructions. The extracted total RNA was given to  company for highthroughput sequencing. The following detailed experimental methods were provided by the company. Eighteen cDNA libraries were constructed using purified mRNAs. The libraries were then sequenced using an Illumina HiSeq2500 machine according to the Illumina protocols