title: GSM5640180: MCF7 D_3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the Universal RNA Purification Kit (E3598; Roboklon) incorporating a DNA digestion step using the RNase-Free DNase Set (79254; Qiagen). RNA quantity, integrity, and quality was measured using a Bioanalyser. RNA was diluted in RNAse-free H20 to obtain 40 µl of 40 ng/ml RNA and was prepared according to the DKFZ manual. RNA libraries were prepared for sequencing using standard Illumina protocols