title: Iso-seq of wohlfahrtia magnifica: the mixed different developmental stages and sexes
design: The mixed mRNA was isolated and reverse-transcribed into full-length cDNA using SMARTer PCR cDNA Synthesis Kit (Clontech, USA). The SMRTbell library was constructed using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, USA). The prepared library was sequenced on the PacBio Sequel II platform.