title: Pipilo ocai MLZ55361
design: We extracted DNA from a small fragment of the toepad of each specimen. We digested the samples and extracted DNA using phenol-chloroform (Tsai et al. 2020) , using negative controls and strict protocols for preventing cross-contamination, including carrying out the extraction in a clean room using dedicated equipment and consumables. We performed library preparation using a Kapa kit (Kapa Biosystems, Inc.) with custom index tags (Glenn et al. 2019) . We pooled 8 samples for target enrichment using the tetrapod 5K ultraconserved element (UCE) probe set (Faircloth et al. 2012) . We combined the enriched pools at equimolar ratios and sequenced on a partial lane of an Illumina HiSeq 2500 using 125 base pair, paired-end (PE125) sequencing. We obtained a total of 94 million read pairs with an average of 2.9 million read pairs per sample.