title: GSM5640789: luc_ChIRPseq_rep3; Arabidopsis thaliana; OTHER
library_construction_protocol: The antisense oligonucleotide probes Even and Odd were designed against the full-length FLAIL sequence, The probes were biotinylated at 3′ end. Antisense oligonucleotides targeting luciferase RNA were used as negative control. Crosslinked seedlings (2.5 g) were ground and suspended in 10 ml Honda Buffer. After RNA immunoprecipitation, DNA was extracted using ChIP DNA Clean & Concentrator (Zymo Research) and used for subsequent DNA sequencing. ChIRP-seq libraries were constructed using the ChIP-seq Library Prep Kit (NOVA-5143-02 with NEXTflex® ChIP-seq Barcodes-24) following the manufacturer's protocol and quantified on Agilent 2100 Bio-analyzer.  ChIRP-seq libraries were pooled into one flow cell (NextSeq 500/550 High Output Kit v2.5) for sequencing with single-indexed, PE (2*75 bp) and sequenced on an Illumina Nextseq 500.