title: RNA-Seq of Neocalanus flemingeri nauplii: 2016  no food 65
design: Total RNA was extracted from individual nauplii (n=12) using QIAGEN RNeasy Plus Mini Kit (catalog # 74134) in combination with a Qiashredder column (catalog # 79654) and shipped on dry ice to the Georgia Genomics Bioinformatics Core (https://dna.uga.edu). There double-stranded cDNA libraries (NEXTERA SMART seqv4 kit) were multiplexed and sequenced using Illumina Next-Seq 500 instrument (High Output Flow Cell, 75 bp, paired end).