title: GSM6672310: Cystic 4; Homo sapiens; RNA-Seq
library_construction_protocol: Extrahepatic dilatations were collected, and all specimens were stored in liquid nitrogen after excision. Total RNAs was isolated from frozen tissues using TRIzol (Invitrogen), DNA digestion was carried out by DNaseI. RNA quality was measured by examining A260/A280 with a NanoDrop (Thermo Fisher) and quantified by Qsep-100 (Bioptic). Then, RNA samples were stored at - 80 °C until further use. 2 μg total RNAs were used to prepare KC-Total RNA-seq Library Prep Kit for Illumina® (Catalog NLR086-01, Wuhan Seqhealth Co., Ltd. China)