title: GSM6723870: RTR61 cells, total RNA, siSNHG6, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from total cell lysates was isolated using the NucleoSpin RNA kit (Machery Nagel) or RNeasy Mini kit (Qiagen). Cytoplasmic fractionation was done using the NucleiEZ nuclei isolation kit (Sigma-Aldrich), and RNA was then purified using Trizol (Invitrogen) according to the manufacturer's protocol. Polyadenylated RNA was purified from total RNA using poly-T oligo-attached magnetic beads. The first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. Illumina PE150 technology was employed to sequence the samples.