title: RNA-Seq of Biomphalaria pfeifferi: whole snail
design: RNA extracted using TRIzol method with PureLink RNA Mini Kit (ThermoFisher Scientific). Quality and quantity was evaluated on a Bioanalyzer 2100 (Agilent Technologies) and Nanodrop 2000 (ThermoFisher Scientific). Illumina cDNA synthesis kits (Invitrogen) were used and libraries were barcoded using universal TruSeq adapters (Illumina) and PCR performed with the KAPA HiFi Hot Start ReadyMix (KAPA Biosystems). Quality and quantity checked on Bioanalyzer 100 and by qPCR.