title: Rhizosphere Fungi of Coffea arabica, 600 m, Shade: ITS
design: Total genomic DNA was extracted from Coffee arabica Soil. Amplification of the 16S rRNA regions was performed using a PCR approach specifically designed for Illumina instruments. The PCR reaction was purified and barcoded amplicons were pooled in equimolar concentration for sequencing on the illumina Miseq platform.