title: GSM6723899: LIVER,KO,REP1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from liver using the Monarch total RNA miniprep kit (New England Biolabs) according to manufacturer's instructions Quality checked RNA was used for library preparation with Illumina stranded mRNA Prep ligation kit (Illumina, #20040532). Libraries were then Concentrated and fragmentated using the Qubit fluorimeter (Invitrogen) and Tape Station system (Agilent) with developer kits (Qubit dsDNA HS Assay Kit, 500 assay, Thermo fisher, # Q32854) (ScreenTape D1000, #5067-5582, Reagents ScreenTape D1000, #5067-5583, Agilent) according to developers' instruction