title: GSM6677866: KO_CD_F_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA is prepared from tissue using QIAzol/chlorofrom extraction and QIAGEN RNeasy Pls Universal Mini Kit (following manufaturer's suggestions). Homogenization was accomplished using a metal bead and a TissueRuptor. RNA is eluted in Invitrogen RNase-free water. Tissues were freshly harvested from the mice, rinsed well in ice cold DPBS (no Mg/Ca), and cut in smaller pieces and rinsed further. The lymph nodes were removed from the inguinal white adipose tissue (iWAT). Tissues were flash frozen in LN2. Tissues were kept at -80C storage until processing. Processing consisted of cryogenic crushing of whole liver or whole iWAT to generate a "powder" of the the whole organ. This powder was aliquoted based on weight and stored. Tissue remained at cryogenic temperatures at all times. 100mg of iWAT was used for RNA isolation and 50mg of liver was used for RNA isolation. Libraries were constructed by Novogene using poly-T magnetic beads following by fragmentation, cDNA synthesis, adaptor ligation, and finally PCR. Quality control was conducted by removing low quality reads or those containing the adaptor or poly-N strings. Quality control was done by Novogene on intial samples, constructed library, and raw data.