title: fungal rhizosphere of wheat field during Germination
design: DNA extraction- soil DNA was extracted from 0.5 g of soil sample using the GeneAll ExgeneTM mini Kit (Seoul, Korea), according to the manufacturer's instructions.   DNA amplification - to amplify and find the fungal DNA, a pair of primers were used in order to express ITS region. DNA was amplified using PCR using SimpliAmpTM thermal cycler (Thermo Fisher Scientific, Walham, MA, USA), by mixing 12.5 L HS Taq Mix Red (PCR Biosystems, London, UK), 9.5 L ultrapure water, 1.0 L extracted DNA, 1 L CS1-ITS2, and 1 L CS2-ITS4. The thermal cycling program was set to: 95 C for 2 min, followed by 40 cycles at 95 C for 15 s, 50 C for 30 s, 72 C for 30 s, and after the cycles, 72 C for 3 min. The amplified DNA was stored at 20 C until sequencing. Sequencing (Miseq) was performed at the Hylabs Laboratory Ltd. (Rehovot, Israel), (www.hylabs.co.il) sequencing facility using an Illumina sequencing platform (Illumina Inc., San Diego, CA, USA).