title: GSM5662729: rRNA-depleted total RNA-seq Piwi GKD rep 2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA-seq: approximately 200 ovaries from 0- to 1-day old Ctrl, Piwi-GKD, HP1a-GKD, and double-GKD females were manually dissected in cold PBS. RNA (from two biological replicates) was isolated using Trizol reagent (Invitrogen). RNA-seq: rRNA was depleted using Illumina Ribo-Zero Plus rRNA Depletion Kit. cDNA library preparation with random primers, and single-end deep sequencing was carried out on the Illumina HiSeq 2000 platform.