title: PacBio long-read sequencing of Poeciliopsis prolifica genome: adule male body
design: PacBio single-molecule real-time (SMRT) library was prepared on 20 g male P. prolifica HMW genomic DNA using SMRTbell prep kit following the whole genome sequencing libraries protocol (Pacific Biosciences, Menlo Park, CA, USA) and sequenced in a total of eight SMRT cells on a PacBio Sequel machine at the HudsonAlpha Genome Sequencing Center (HudsonAlpha Institute for Biotechnology, Huntsville, AL, USA).