title: GSM6589216: HCT116, MLL4 KO, RNA, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: 1 μg total RNA was subjected to mRNA purification using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490). Isolated mRNAs were reverse-transcribed into double stranded cDNA and subjected to sequencing library construction using the NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) according to the manufacturer's instructions.