title: Congenital hemolytic anemia due to AK1 deficiency
design: We performed library preparation using Illumina's TruSeq Custom Amplicon v1.5 kit (FC 130 1001) using 250 ng genomic DNA, following the manufacturer's instructions. Samples were pooled and loaded at 20 pM on MiSeq using a v3 600 cycle reagent kit sequencing 2301 pairedend reads (Illumina, San Diego, CA, USA). The library was sequenced to mean >80-100X coverage on the Illumina MiSeq sequencing platform.