title: unenriched RNAseq bosRmut rpoSmut irpoS no IPTG EcAG646 DMC # 2
design: Total RNA was isolated from B. burgdorferi B31 bosR/rpoS double mutant with inducible rpoS cultivated within Dialysis Membrane Chambers implanted into rat peritoneal cavities. Libraries were prepared using Stranded Total RNA Ligation kit (Illumina), which includes ribodepletion, according to manufacturers instructions. Libraries were run on an Illumina NovaSeq6000.