title: RNA-seq of Lactobacillus johnsonii N6.2: Cellular RNA replicate 2
design: Library construction and sequencing was performed by Novogene, (Novogene Co., Davis, CA, USA) as follows. RNA purity was checked using the NanoPhotometer spectrophotometer (IMPLEN, CA, USA), and RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 1 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated. Raw data (raw reads) of FASTQ format were first processed through fastp. The reference genome (NCBI NC_022909.1) and gene model annotation files were downloaded from NCBI.