title: Control-uninfected 4
design: After obtaining the results of the diagnosis of Perkinsus spp., hemocytes suspensions were pooled in order to have 4 pools (P) of 10 oysters each from heavily infected and uninfected (control) oysters, further assigned as IP and CP, respectively. Hemocytes suspensions were pooled using oysters from all samplings times and considering similar: a) hemocytes concentration ( 1.5 x 106 mL-1) established by flow cytometry, b) oysters size (length was not significantly different between infected oysters: 81.4  0.82 mm and control oysters: 79.5  1.02 mm), c) female: male ratio (HI: 2.1, UI: 1.5), and d) Perkinsus spp. proportion (68% P. marinus and 32% P. beihaiensis). Total RNA was extracted from 8 pools of hemocytes using the Extract all kit (Eurobio, France). The quality and quantity of RNA were analyzed in Bioanalyser and Nanodrop. Total RNA (5 g) extracted from the 8 pools were sent to ACOBIOM (Montpellier, France) for sequencing. The eight cDNA hemocytes libraries (4 CP and 4 IP) were constructed following the TruSeq Stranded mRNA protocol and sequenced on NextSeq (Illumina) platform by paired-end (2x150bp) using 1 g of total RNA by the ACOBIOM company (Montpellier, France) for sequencing.