title: GSM6589298: Male_WT-vehicle-rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the corneas of mice using Trizol-chloroform based extraction and then RNA purification using RNeasy column (Qiagen) according to the manufacturer' protocols.  Each experimental group had 4 replicates, where each replicate had 4-6 corneas pooled into one sample. Indexed samples were sequenced using the 75bp pair-end protocol using the Illumina NextSeq500 sequencer as per the manufacturer's protocol. Reads greater than 40 million reads per sample were aligned to the reference genome UCSC mm9.