title: GSM5509922: RNA1-flg22_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Protoplasts were isolated from 4-week-old wild type Arabidopsis plants, and then resuspend the protoplasts in WI solution at a density of 1 x 10^6 /ml. For ATAC-seq, lysed protoplasts and collected the nuclei, the pellet was incubated with Tn5 for later to construct sequencing libraries. Ribosomal RNA (rRNA) depletion bulk RNA-seq to determine whether enhancer drives RNA synthesis at enhancers. Ribosomal RNA was depleted using Ribo-Zero kit. Equal amounts of RNA from biological replicates were pooled for RNA-seq library construction.