title: 16S amplicon sequencing of lake water
design: Samples were processed as described in Chavez-Daz et al. (2020). Genomic DNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany). The V4-V5 region of the 16S/18S rRNA gene was amplified using the 515FY (5-GTGYCAGCMGCCGCGGTAA) - 926R (5-CGYCAATTYMTTTRAGTTT) primer set. PCR was run using the following protocol: initial denaturation at 95C for 2 min, 35 cycles of amplification (95C for 20 s, 50C for 30 s, 72C for 60 s) and a final elongation at 72C for 5 min.