title: Waus_HiC
design: For the Hi-C data, freshly prepared samples were chopped into 2-cm pieces and vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Crosslinking was stopped by adding glycine and additional vacuum infiltration. Fixed tissue was then grounded to powder before re-suspending in nuclei isolation buffer to obtain a suspension of nuclei. The purified nuclei were digested with 100 units of DpnII and labelled by incubating with biotin-14-dCTP. Biotin-14-dCTP from non-ligated DNA ends was removed because of the exonuclease activity of T4 DNA polymerase. The ligated DNA was sheared into 400-bp fragments, and then was blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down.