title: Draft genome resources for Chicken Coccidiosis Causal Agents for developing improved control strategies for Avian Coccidosis
design: High-quality DNA (approximately 100 to 200 ng) was obtained from pure mycelial cultures using the Qiagen Kit protocol for DNA extraction, modified to be used for Eimeria spp. The quality of the genomic DNA was determined using Qubit, Nano- drop, and Tape station (Agilent Technologies) and the genomic DNA libraries were prepared from 100 to 200 ng using the ONT ligation sequencing kit SQK-LSK109 raw reads were generated on a flowcell R9.4.1 in a MinION device for 48 h using ONTs sequencing protocol. The Illumina reads were generated as per standard Illumina protocol Using HiSeq 2500 Instruments.