title: GSM6153713: Day7 MP Malat1 Knockdown isotype, CUT&RUN; Mus musculus; OTHER
library_construction_protocol: One-hundred thousand TE and MP Malat1 Knockdown and Nontarget P14 CD8+ T cells were sorted 7 days after LCMV CUT&RUN libraries generated using the Cell Signaling CUT&RUN Assay kit according to manufacturer protocol (Catalog Number: 86652). Equal numbers of 100,000 cells per sample condition were isolated for isotype controls. Binding with primary anti-H3K27me3 antibody (07-449, EMD Millipore) and IgG control antibody binding was conducted overnight at 4°C. Fifty picograms of Spike-In Yeast DNA was added per sample after pAG-MNase activation with Calcium Chloride. DNA was purified and sequencing libraries generated using SimpleChIP ChIP-seq DNA Library Prep Kit for Illumina with Dual indices according to manufacturer protocol (Catalog number: 56795). Final libraries were amplified for 10 cycles.