title: Bacillus subtilis stationary culture transcriptome
design: Adaptive laboratory evolution was employed in B. subtilis BS011 by gradually increasing the concentration of menadioneMK-7 analogue, and subsequently, the evolved strain BS012 was obtained. B. subtilis BS011 and BS012 were cultured in the fermentation medium in 500 ml flasks with 100 ml fermentation medium and grown at 40C with shaking at 220 rpm for 60 hours. The total RNA of these strains was extracted, synthetic the first cDNA and DNA sequencing were performed on a Novaseq 6000 sequencing system By GENE DENOVOS (Guangzhou, China).