title: GSM6368882: HEP_HFHS_Set2_1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were collected, cells were lysed, suspended and barcoded using optimized protocols for hypothalamus (n=2 control, 2 fructose, 2 HFHS samples)23, gonadal adipose stromal vascular fraction (SVF) (n=2 control, 2 fructose, 2 HFHS samples)25, small intestine (n=2 control, 2 fructose, 2 HFHS samples)26, liver non-parenchymal cells (NPCs) (n=3 control, 5 fructose, 4 HFHS samples)27 and hepatocytes (n=2 control, 4 fructose, 4 HFHS samples) (http://mouselivercells.com/procedure.html) and version 3.1 of the online Drop-seq protocol (http://mccarrolllab.com/download/905/). Sequencing libraries were prepared according to the Drop-seq protocol and sequenced using Illumina HiSeq4000 with 50bp paired-end setting at 20-50k reads/cell.