title: pmoA amplicon sequencing of riverine sediment
design: Extracted DNA was amplified as published previously (van de Kamp et al., 2019) using the primers pmoA189GC and pmoA682 (Holmes et al., 1995) with Illumina overhang adapters (IA-pmoA189GC: TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGGNGACTGGGACTTCTGG and IA-pmoA682: GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGAASGCNGAGAAGAASGC). pmoA amplicon products were purified and sent to Ramaciotti Centre for Genomics (UNSW Sydney, Australia) where second stage PCR incorporating Nextera XT barcodes, purification, library generation and sequencing using the Illumina MiSeq platform (Illumina, Inc., San Diego, USA), with 300 bp paired reads, was performed according to manufacturers directions.