title: RNAseq of Zea mays ears: ramosa2 multiplexed by BRBseq
design: A family of ra2 plants were grown in standard greenhouse conditions and a series of developing ears spanning initial patterning to floral differentiation was collected. RNA was extracted using Trizol, quantified by fluorometry, then prepared using the BRBseq multiplexing strategy (Alpern et al. 2019). The final library was sequenced using a HiSeq 3000 and 100PE chemistry.