title: GSM5640657: 30_72h_1262_stretch_3_S28; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using a NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer's instructions. Cells were lysed in 350 µl of RA1 lysis buffer supplemented with 1% β-mercaptoethanol and stored at -80 °C (maximum one month) before RNA isolation. The RNA quality was analyzed with Agilent 4200 TapeStation. Library was constructed using NEBNext Ultra Directional RNA Library Prep kit including rRNA depletion.