title: BacCapRNAseq rpoScomp BbP1754 DMC # 3
design: Total RNA was isolated from B. burgdorferi B31 rpoS-complement cultivated within Dialysis Membrane Chambers. Libraries were prepared with the Kapa Hyperplus kit (Roche). To enrich for Bb-specific transcripts, library pools were mixed with custom biotinylated TBD SeqCap EZ Probe pool (Roche) containing overlapping biotinylated probes designed for strain B31. Borrelia-enriched material was recovered using SeqCap Pure streptavidin capture beads (Roche) and amplified for 16 cycles of PCR using Illumina universal primers (Kapa HiFi HotStart Ready Mix, Roche). The amplified pool was sequenced on an Illumina NextSeq2000 platform.