title: DNA-Seq of human: embryonic stem cell
design: Libraries were constructed using the Kapa HyperPlus Kit. Briefly, up to one ug of EDTA-free dsDNA was incubated with the fragmentation enzyme for ten minutes at 37 degrees. The fragmented samples were then end-repaired and A-Tailed and Illumina indexed adapters were ligated onto the ends of the dsDNA. The libraries were then size selected using Ampure XP beads (Beckman Coulter) using a 0.65 Ampure XP to DNA ratio followed by a 0.9 ratio to achieve an average library size of about 400 bp. Due to small input amounts for several of the libraries, library amplification (4 cycles) was performed on a selection of samples.