title: GSM6722667: H2Aub-Col, rep2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Chromatin was extracted from 1% formaldehyde fixed tissue and fragmented using a Bioruptor® Pico (Diagenode) in fragments of 200–500 bp. For IP the antibodies used were H2Aub (Cell Signalling Technology D27C4) and H3K27me3 (Millipore 07-449). BGISEQ platform Chip-seq library preparation:  1) End repair and adapter ligation:Fill the ends of IP/Input DNA followed by 5' phosphorylation. A 3' sticky-end with an overhanging A is formed, which could ligate with an overhanging T at the 3' end of the bubble adapter;  2)PCR amplification: PCR amplify the ligated products with specific primers;  3)Single-strand separation and circularization: Separate PCR products into single-strands by thermal denaturation. Then circularize the single-stranded DNA through a bridge primer to obtain single-stranded circular DNA library;  4)Sequencing: Amplified products DNA Nanoballs (DNBs) are used for sequencing.