title: RNA-Seq of Halichoanolaimus dolichurus sp.: 6 individuals
design: Total RNA was extracted using the Ambion RNAqueous Micro Kit following the manufacturers protocol. Subsequent library preparation and cDNA synthesis was performed using the Takara SMART-Seq HT kit following the manufacturers instructions. The resulting double-stranded cDNA was purified using Ampure XP magnetic beads. Final sequencing library preparation was performed using the Illumina Nextera XT kit.