title: K18-hACE2-mouse lung IM immunized WA-1 challenged
design: Lungs from WA1-SARS-CoV-2 were homogenized using gentle MACS dissociator and TRI reagent was added to the homogenate.  RNA was purified from TRI reagent using Direct-zol RNA miniprep.  RNA was quantified by Qubit and TapeStation.  RNAseq libraries were prepared with KAPA RNA HyperPrep Kit with RiboErase for RNAseq analysis.