title: GSM6675893: CRC, GDT, CITE-seq; Homo sapiens; OTHER
library_construction_protocol: Single-cell digests of MMR-deficient colon cancer specimens were thawed, stained with cell surface flow cytometry antibodies and HTOs, and washed. Single, live CD45+ EPCAM– CD3+ TCRγδ+ cells were sorted on a FACS Aria III 4L (BD Biosciences). After sorting, the samples were pooled. scRNA-seq libraries were prepared using the Chromium Single Cell 5' Reagent Kit v1 chemistry (10X Genomics) following the manufacturer's instructions. The construction of 5' Gene Expression libraries allowed the identification of γδ T cell subsets according to Vδ and Vγ usage. Libraries were sequenced on a HiSeq X Ten using paired-end 2x150 bp sequencing (Illumina).