title: RNA-seq of human myoblast
design: Final libraries were then pooled to 10 nM, and the concentration of the pool was determined using the Kapa Biosystems Universal qPCR kit (Roche, Wilmington, MA). After dilution to 300 pM and denaturing with NaOH, the pooled library was loaded onto a NovaSeq6000 SP flow cell (PE50; Illumina, San Diego, CA) for sequencing.