title: GSM6675529: S12.5d_8; Mus musculus; RNA-Seq
library_construction_protocol: The tissues were cut into 4-5 mm pieces and treated with cleanroom wipers. After removing blood stains on the tissue surface, fresh tissue was embedded with OCT then snap frozen. Cryosections were cut at 10-um thickness, mounted onto the ST arrays.Next, the tissue was dehydrated with isopropanol for 1 min followed by staining with HE. Brightfield images were taken on a Leica SCN400 F whole-slide scanner at 40x resolution. Library preparation slides used were purchased from the Spatial Transcriptomics team. Each of the spots printed onto the array is 55um in diameter and 100um from center to center, covering an area of 6.5x6.5 mm2. Each slide includes 4 Capture Areas, each with ~5,000 unique gene expression spots.