title: Complete genome of Erwinia amylovora strain 99east-3-1
design: Cells from 15 mL bacterial suspension were harvested by centrifugation, and genomic DNA was isolated using SDS lysis and purification column. The genomic DNA was submitted to Tsingke Biotechnology (Beijing, China) for Illumina sequencing after test for concentration, purity and integrity. The DNA was randomly broken by Covaris M220, and then the whole library was prepared by end repair, adding A tail, adding sequencing connector, purification, PCR amplification and other steps. Qubit 2.0 was used for quantification and the library dilution. The inserted fragments of the library were detected by Agilent 2100. Then the size of the inserted fragments met the expectations, qPCR was used to accurately quantify the effective concentration of the library to ensure the quality of the library. The DNA libraries were prepared utilizing a single library preparation method of SQK-LSK109, EXP-NBD104 and 114 kits (Oxford Nanopore)based on the Illumina Nextera kit and were sequenced on the NovaSeq 6000 platform.