title: GSM6153069: FLFL_TREAT_PILEUP, input; Mus musculus; ChIP-Seq
library_construction_protocol: 2 × 107 cells were crosslinked in 1% formaldehyde for 10 minutes at room temperature, and quenched with 0.125M glycine for at least 5 minutes. Cells were washed twice with 1 x PBS, and scraped into ice-cold 1 x PBS supplemented with protease inhibitor cocktail. Cells were snap frozen at this point and stored at - 80°C until further use. DNA libraries were prepared using TruSeq ChIP sample prep kit (Illumina) and sequenced on a NextSeq 550 (Illumina) platform.