title: GSM7006762: spleen, apoptotic allo, rep1 [AA]; Mus musculus; RNA-Seq
library_construction_protocol: spleens were collagenase D and DNAse I digested and subjected to independent CD45+ and pan DC magnetic enrichment and re-pooled at a 1:1 ratio per condition Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10x Genomics). Briefly, single cells from splenic cell suspenion were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.