title: ITS2ampliconseq_mouse
design: Genomic DNA and RNA was extracted from the faecal and gut tissue using sterile Lysing Matrix E bead tubes (MP Biomedicals, Seven Hills, NSW, Australia) and the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany). The V3-V4 region of the bacterial 16S rRNA gene was amplified using the primers 341F and 785R together with Nextera DNA library prep kit adapters (Illumina, CA, United States).