title: D06MG-NSC Whole Exome Sequencing
design: Genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument.  Fragmented DNAs were cleaned up and end repaired, as well as adenylated at the 3ends.  Adapters were ligated to the DNA fragments, and adapter-ligated DNA fragments were enriched with limited cycle PCR.  Adapter-ligated DNA fragments were validated using Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified using Qubit 2.0 Fluorometer.  Adapter-ligated DNA fragments were hybridized with biotinylated baits.  The hybrid DNAs were captured by streptavidin-coated binding beads.  After extensive wash, the captured DNAs were amplified and indexed with Illumina indexing primers. Post-captured DNA libraries were validated using Agilent TapeStation (Agilent, Santa Clara, CA, USA) and quantified using Qubit 2.0 Fluorometer and Real-Time PCR (KAPA Biosystems, Wilmington, MA, USA).