title: RNA-Seq of Dermacentor nitens: adult male gut
design: The DNA samples were prepared for targeted sequencing with the Quick-16S NGS Library Prep Kit, Primer Set V3-V4. The sequencing library was prepared using PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final library was sequenced on Illumina MiSeq with a v3 reagent kit (600 cycles). The sequencing was performed with >10% PhiX spike-in. Unique amplicon sequences were inferred from raw reads using the Dada2 pipeline.  Chimeric sequences were also removed with the Dada2 pipeline. Taxonomy assignment was performed using Uclust from Qiime v.1.9.1.