title: II-5-WES
design: DNA was extracted with a PureLink Genomic DNA Mini Kit (Invitrogen, United States). Exome capture, high-throughput sequencing were delivered to the IGeneTech Co., Ltd). All the exomes were captured using the Bioruptor Pico (Bioruptor, United States). and were sequenced by an Illumina HiSeq2500 platform. Filtering strategies were the same as our previous study. The Genome Analysis Toolkit (GATK) was used for variant analysis in the target regions.