title: amplicon V3-V4 sequencing from seaweed fly larvae
design: Libraries were made using the protocol from the Andersson lab (https://github.com/EnvGen/LabProtocols/blob/master/Amplicon_dual_index_prep_EnvGen.rst). After sequencing and de-multiplexing, primer and adaptor removal, as well as trimming, were done using cutadapt enabling wildcards in reads and specifying that the first five bases of each read should be trimmed.