title: sequences of bacteria
design: Raw fastq files were first demultiplexed using in-house perl scripts according to the barcode sequences information for each sample with the following criteria: (i) The 250bp reads were truncated at any site receiving an average quality score <20 over a 10 bp sliding window, discarding the truncated reads that were shorter than 50bp. (ii) exact barcode matching, 2 nucleotide mismatch in primer matching, reads containing ambiguous characters were removed. (iii) only sequences that overlap longer than 26 bp were assembled according to their overlap sequence. Reads which could not be assembled were discarded