title: whiskers deprivation
design: RNA-seq transcriptome librariy was prepared following TruSeqTM RNA sample preparation Kitfrom Illumina (San Diego, CA) using 1g of total RNA. Shortly, messenger RNA was isolatedaccording to polyA selection method by oligo(dT) beads and then fragmented by fragmentationbuffer firstly. Secondly double-stranded cDNA was synthesized using a SuperScriptdouble-stranded cDNA synthesis kit (Invitrogen, CA) with random hexamer primers (Illumina).Then the synthesized cDNA was subjected to end-repair, phosphorylation and A base additionaccording to Illuminas library construction protocol. Libraries were size selected for cDNA targetfragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using PhusionDNA polymerase (NEB) for 15 PCR cycles. After quantified by TBS380, paired-end RNA-seqsequencing library was sequenced with the Illumina HiSeq xten/NovaSeq 6000 sequencer (2 150bp read length).