title: Subject 7, prednisolone 50 nM and idelalisib 500 nM, replicate 2
design: Mononuclear cells were isolated by Histopaque density gradient separation and immediately treated as indicated for 24 hours in RPMI 1640 + 10% FBS media at 37°C, 5% CO2. RNA was extracted from 2 million cells using miRNeasy Mini Kit (Qiagen cat#217004). RNA quality was assessed using Agilent Bioanalyzer. Libraries were prepared with Illumina TruSeq Stranded mRNA kit by Iowa Institute of Human Genetics Genomics Division. Adapters were trimmed using Trim Galore. Trimmed reads were mapped to Gencode Fasta transcriptome GRCh38. Counts were imported into R using tximeta, with differential expression performed using DESeq2.