title: WT replicate 5
design: The V4 region of the 16S rRNA was PCR amplified and libraries were prepared for sequencing using standard Illumina 16S Metagenomic Sequencing Library Preparation protocols. Paired-end reads were analyzed and classified into operational taxonomic units (OTUs) at > 97% identity level using Mothur v.1.40.5. Taxonomic assignments were determined using the SILVA 16S rRNA reference file release 132 and the Ribosomal Database Project (RDP) training set version 16.