title: RNAseq of human naive Treg cells
design: Cell subsets were sorted from blood. RNA was isolated using the RNeasy Micro Kit (Qiagen). cDNA libraries were prepared with SMART-Seq v4 Ultra Low Input Kit for Sequencing (Takara Bio). The samples were sequenced using a HiSeq 4000 (75 bp, 10 million average paired reads per sample).