title: GSM6153688: 8h_Vneg_LCMV_M5 [48]; Mus musculus; RNA-Seq
library_construction_protocol: BMDMs were cleared out of supernatants and lysed with 350 µl RLT buffer (Qiagen) supplemented with 40 mM DTT (Dithiotreitol) for RNA extraction. Plates were then frozen at -80°C until the RNA purification. The RNA samples were purified with the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's instructions. Purified RNA was eluted with 30 µl RNase-free water (Qiagen). Library preparation and sequencing were performed at the Lausanne Genomic Technologies Facility (GTF), University of Lausanne (UNIL), Switzerland