title: Col-0 ChIP
design: change double-strand DNA to single-strand DNA by high temperature incubation, and keep single-strand DNA structure unfolded. Then connect T7 Adaptor to the 3'end of single-strand DNA quickly and efficiently. Use DNA elongase to complete the synthesis of Single-strand DNA complementary strands, which also has high efficiency for the synthesis of single-stranded templates containing dUTP. After connect T5 Truncated Adapter to the 5'end of Double Stranded DNA. At last, adapter ligation DNA was PCR amplified with primers for 15 cycles and library fragments of ~250 bp.