title: 16S rRNA gene sequencing for Prochlorococcus sp.RSP50 Co-culture incubated at 30C day 4
design: Sequencing library of heterotrophic bacterial community of Synechococcus sp. RS9907 co-culture samples were prepared following the Illumina 16S Metagenomic Sequencing Library Preparation guide. Briefly, we amplified variable region 4 and variable region 5 of the 16S rRNA gene with the following primers the forward primer 515F-Y (5-GTGYCAGCMGCCGCGG TAA)  and reverse primer 926R (5-CCGYCAATTYMTTTRAGTTT). Then we performed Indexing PCR on the cleaned samples using Nextera XT index kit (Illumina, San Diego, CA, USA) following the manufacturers protocol. 16S rRNA gene amplicon library was sequenced using Illumina MiSeq platform in paired-end mode and a 20% phiX as control.