title: GSM6153044: Differentiated Mc; Mus musculus; RNA-Seq
library_construction_protocol: For Activated McSC sample, melanocyte stem cells were isolated from the back skin of Dct-rtTA; tetO-H2B-GFP mice at 4 days after depilation. For Differentiated_Mc sample, the hair bulb area of Anagen VI hair follicles from the back skin of 5 weeks old Dct-rtTA; tetO-H2B-GFP mice wre dissected out add dissociated into single cells. FACS for GFP+ cells were utilized to enrich for melanocytes. Single-cell RNA-Seq library for the Differentiated_Mc sample was prepared using the following Chromium Single Cell 3' v2 Reagent Kits: Chromium™ Single Cell 3′ Library & Gel Bead Kit v2 PN-120237; Single Cell 3′ Chip Kit v2 PN-120236 and i7 Multiplex Kit PN-120262 (10x Genomics) following the Single Cell 3′ Reagent Kits v2 User Guide (Manual Part # CG00052 Rev A). Single-cell RNA-Seq library for the Activated_McSC sample was prepared using the following Chromium Single Cell 3' v3 Reagent Kits: Chromium™ Single Cell 3′ Library & Gel Bead Kit v3 PN-1000075; Single Cell 3ʹ Feature Barcode Library Kit PN-1000079; Single Cell B Chip Kit PN-1000073 and i7 Multiplex Kit PN-120262 (10x Genomics) following the Single Cell 3′ Reagent Kits v3 User Guide (Manual Part # CG000201 RevA).