title: 16S rRNA gena amplicon sequencing of soil: Pristine soil, 60 days, Replicate 1
design: Microbial 16S rRNA genes were amplified using primers 515F and 806R targeting the V4 region. Nextera 2 nd step PCR incl. pooling. Sequencing on Illumina MiSeq, v2, 2x250 bp.