title: Genome Sequencing of Seven B2 clade Escherichia coli Associated with Recurrent Urinary Tract Infections in Post-Menopausal Women
design: The DNA was isolated following the protocol provided by New England BioLabs for gram-negative bacteria via the Monarch genomic DNA purification kit protocol. Once collected, DNA was submitted to SeqCenter (Pittsburgh, PA) for hybrid sequencing. Sample libraries were prepared using Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-LSK109) with NEBNext Companion Module (E7180L) in addition to Native Barcode Kits (EXP-NBD104, EXP-NBD114) to manufacturers specifications. All samples were run on Nanopore R9.4.1 flow cells and MinION Mk1B device.