title: SgRNA analysis
design: Genomic DNA was extracted from the uninfected cells or the survived cells after PRV infection, and sgRNA sequences were amplified amplified by PCR using the primers.  A second PCR was to fix barcodes, index, and adaptor sequences into the amplicons. Targeted next-generation amplicon sequencing (NGS) was performed using Illumina PE150 sequencer (Novegene, Beijing, China). The sgRNA sequences against specific genes were recovered after removal of the tag sequences using the AdapterRemoval (version 2.1.7)