title: GSM6265796: NM1 KO MEF 03; Mus musculus; RNA-Seq
library_construction_protocol: RNA from both tumors and cells were isolated by TRIreagent according to manufacturers' protocol. Total RNA from 4 replicates of NM1 KO MEFs was extracted with TRI Reagent (Millipore-Sigma) according to the manufacturer's protocol. For transcriptional profiling of tumor tissues, the biggest (fast-growing tumor, TF), and the smallest (slow growing tumor, TS) tumors were selected for comparison. Each tumor was divided to 4 equal pieces and each piece separately homogenized in TRI Reagent by using the Bead Ruptor 96 Homogenizer (Omni International). The RNA-Seq library was prepared by using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with the NextSeq 500/550 sequencing platform (performed at the NYUAD Sequencing Center).