title: GSM6971262: Patient66_Sample4; Homo sapiens; RNA-Seq
library_construction_protocol: Nasopharyngeal swabs were collected from each participant using a minitip flock swab (VWR catalog no. 10755-196; Copan Diagnostics) and immediately placed in a 15 mL tube containing 2 mL of DNA/RNA Shield (Product no. R1100-250; Zymo). Samples were separated into two 1 mL aliquots, and stored at −80°C. After thawing, RNA extraction was done using Zymo Quick-RNA™ Microprep Kit RNA extraction kit (ZYMO, R1050) including the recommended on column DNase I treatment step for 15 min. An Agilent Bioanalyzer was used for quality control prior to library creation For single end samples: RNA RIBOZERO 30M SE100. For paired end samples: RNA RIBOZERO 20M PE100. Then proceed with library construction using Illumina TruSeq  chemistry.