title: Ind-BM2-p
design: An equal amount of DNA was used for PCR amplifications with two sets of primers in the V4 variable regions for 16s rRNA and ITS1 region for fungi. Sequencing libraries were generated after PCR with equal amount following manufacturers recommendations with a selected DNA fragments of preferentially ~300 bp in length. Products were purified using the AMPure XP system. Libraries were sequenced with the PE250 specification on the Hiseq 2500 platform.