title: PER71 starved of carbon and nitrogen sources (Treatment Day 6 replicate 2)
design: Sample is ground in the lysis buffer and mRNA is isolated from this using oligo dT beads. The mRNA is used to make first and second strands of cDNA and the double stranded cDNA molecules are subsequently enzymatically fragmented. The ends of these molecules are repaired and an A nucleotide is added to facilitate TA ligation of the barcoded adapters. The ligated samples are then enriched by amplification using adapter specific primers and purified for sequencing on the Hiseq 4000 platform.