title: Shotgun metagenome
design: For sample preparation, the Agilent 5400 fragment analyser system was used to quantify the samples and to determine sample integrity and purification. Thereafter genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified.The libraries were checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.This sequencing was performed by Novogene, Singapore