title: Small RNA Seq of Candida albicans + spike-ins
design: We used the NextPARs technique, previously implemented in our lab (PMID: 29358234, PMID: 34141143), to probe the secondary structure of the transcriptomes at 23C, 37C and 55C for C. parapsilosis and at 23C for the rest of the species. Two g of the corresponding polyA+ RNA were used per each reaction tube. 0.03 U of RNase V1 (Ambion) and 200 U of S1 nuclease (Fermentas) were used to digest the corresponding samples when probing the structure at 23C. When samples were probed at 37C and 55C, the final enzyme concentration was adapted to prevent overdigestion of RNAs due to higher temperatures, according to previous studies (PMID: 22981864). Thus, in the digestion reactions, 0.015 U or 0.0075 U of Rnase V1 (Ambion), and 100 U or 50 U of S1 nuclease (Fermentas) were used to probe the transcriptomes at 37C or 55C, respectively. We performed quality controls of the final digested samples to confirm that they were not overdigested after the enzymatic treatment.