title: RNA-seq of zebrafish at 48 hpf : DMSO exposed
design: The first cDNA strand was synthesized in the m-MulV reverse transcriptase system, and then the RNA strand was degraded by RNaseH. The second strand of cDNA was synthesized in the DNA polymerase I system. AMPure XPbeads were used to screen the cDNA from 370 bp to 420 bp. PCR amplification was performed and PCR products were purified again with AMPure XP Beads to obtain the library.