title: RNA-seq of S. cerevisiae: Culture in presence of M. pulcherrima EV at low concentration isolated by exclusion chromatography
design: From 100 ng of total RNA of each sample the TruSeq Stranded mRNA kit (illumina) library was prepared following the manufacturer's instructions (Illumina). The quality of the libraries has been evaluated in Fragment Analizer (AATI) and its concentration has been calculated by fluorimetry (Qubit 3.0). The resulting libraries have been sequenced in a flow cell channel in a Illumina HiSeq1500, using a 100-cycle paired-end reads.