title: GSM6672262: offspring (D28) kidney, control sample, rep4; Rattus norvegicus; Bisulfite-Seq
library_construction_protocol: DNA extraction was performed using the DNAiso Reagent (Takara Bio Inc., Shiga, Japan) according to the manufacturer's instructions. DNA concentration was determined using a NanoDrop® 1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA), and quality was confirmed at OD260/280 > 1.8 and OD260/230 > 1.8. Electrophoresis (100 V, 20 min) using a 1.2% agarose gel was used to check for DNA degradation. DNA concentration was measured again using Qubit (Thermo Fisher Scientific, Waltham, MA, USA). Extracted DNA was kept at -80 °C until later analysis. Whole-genome bisulfite sequencing was performed using the PBAT (Post-bisulfite adapter tagging) method. A DNA library was prepared according to the method of Miura et al., and indexed and read in the paired-end mode for 150 cycles ×2 using a large-scale sequencer (HiSeq X Ten; Illumina, San Diego, CA, USA) at the Macrogen Japan (Tokyo) sequencing laboratory.