title: RNA-seq of mouse: WT 16 days
design: RNA was collected from O4-microbead-purified cells using RNeasy Micro Kit (Qiagen 74004) following the manufacturers instructions. cDNA was prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) and xGen Broad-range RNA Library Prep (IDT, 1009813) with xGen Normalase UDI Primers (IDT). This pool was subjected to 151bp paired-end sequencing according to the manufacturers protocol (Illumina NovaSeq). BCL Convert Conversion Software v3.9.3 (Illumina) was used to generate de-multiplexed Fastq files.