title: RNA-Seq of mus musculus: adult female colonic content
design: Total genomic DNA of colonic content sample was extracted using the DNA extraction kit (Omega Bio-tek, GA, U.S.). After completing the genomic DNA extraction, the extracted genomic DNA was detected using 1% agarose gel electrophoresis. 16S rRNA gene was amplified by PCR using the extracted DNA as a template. Three replicates were performed for each sample. The purified amplicons were sequenced on the Illumina MiSeq PE310 platform (Illumina, San Diego,USA). Bioinformatics analysis of the intestinal flora was performed using the Majorbio Cloud platform (https://cloud.majorbio.com).