title: 16S rRNA amplicon sequencing of riverine sediment
design: Extracted DNA was sent to Ramaciotti Centre for Genomics (UNSW Sydney, Australia) where the v4 region of the 16S rRNA gene was amplified (using primers 515f and 806r; Caporaso et al. 2011, 2012) and sequenced using the Illumina MiSeq platform (Illumina, Inc., San Diego, USA) with 250 bp paired reads.