title: GSM7085895: Root, Cold, 3hours, replicate 2 [Rt_180_Cold_2]; Solanum lycopersicum; RNA-Seq
library_construction_protocol: Total RNA was extracted from 15 shoot samples (two treatments + two timepoints +  three biological replicates + three time zero samples) and 9 root samples (two treatments + three biological replicates + three time zero samples) with RNeasy Plant Mini Kit (Qiagen, USA) following the manufacturer's protocol. The quality and quantity of RNA were checked using the Nanodrop 1000 spectrophotometer (ThermoScientific, USA). RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies). All samples had RIN values >8. The RNA samples were processed, and sequencing libraries were generated using the Illumina TruSeq RNA Sample preparation kit for cDNA library preparation and sequenced on the Illumina HiSeq or NovaSeq platforms (150 cycles, paired end)