title: RNA-Seq of Nicotiana tabacum: Peronospora tabacina infection
design: Tobacco leaf disks were harvested 2 or 5 days after infiltration with Peronospora tabacina sporangia.  The 2 cm disks were punched from the leaf near to the infiltration site and immediately frozen in liquid nitrogen.  RNA was extracted from the disks and RNA sequencing was completed.