title: 10 Chromium long-range contiguity sequencing of Glossina fuscipes fuscipes: whole larva
design: Whole genome sequencing with 10X Chromium GenCode Technology (10x Genomics, USA) from a single  pupa originating from the Yale School of Public Health insectarys colony (IAEA, Vienna, Austria). High molecular weight genomic DNA was extracted from the whole body of the pupa following the 10x Genomics Sample Preparation Demonstrated Protocol DNA Extraction from Single Insects [54], and was suspended in 1X TE Buffer. Genomic DNA was sent to the Yale Center for Genomic Analysis for quality control including a pulse field gel, library preparation, and 10X Genomics Genome Sequencing.