title: GSM6674403: ZcKO _2cell_RNAseq_rep3; Mus musculus; RNA-Seq
library_construction_protocol: GV oocytes were collected 46h after PMSG injection, MII oocytes were collected 14h after hCG injection, and 2cell embryos were collected 32h after insemination. Five oocytes or embryos were collected for each sample.  The RNA-seq libraries for the harvested samples were generated based on Smart-seq2. ERCC spike-ins were added for normalization. Sequence libraries were constructed using the KAPA HyperPlus Prep Kit (KK8514) according to the manufacturer's instructions.