title: fungi
design: We pooled the purified amplicons in equimolar proportions and sequenced them (2 300) using an Illumina MiSeq platform (Illumina, San Diego, CA, USA) applying its standard methods. Trimmomatic and FLASH were used to quality-filter the raw FASTQ files with the following criteria: (1) Any site with an average quality score of 20 or below across a sliding window of 50 bp was used to truncate the 300 bp reads and any truncated reads less than 50 bp were eliminated. Reads containing N-bases were also discarded. (2) Sequences whose overlaps were longer than 10 bp were merged according to their overlaps with any mismatch, not more than 2 bp. (3) Each samples sequences were separated using barcodes (exactly matching), primers (allowing for two nucleotide mismatching), and readings containing ambiguous bases.