title: Dnaseq_long_read_from hppR
design: DNA was extracted from crushed ovaries using the bacterial enrichment procedure by (51). Briefly, tissues were homogenised with a Dounce tissue grinder B in a PBS solution containing sucrose (0.25 M) and L-glutamine (5 mM) to crush cells while keeping the nuclei intact. The homogenate was then passed through a 5 m filter to remove cell nuclei. DNA was extracted from the filtered homogenate using the DNeasy Blood and Tissue kit (QIAGEN, Hilden, Germany). RNA contaminants were removed with RNase A treatment (0.2 g/L at 20C for 2 minutes). 1.2 g of genomic DNA was used for library preparation using the Oxford Nanopore Ligation Sequencing kit SQK-LSK 109 (Oxford Nanopore Technologies, UK). The library was sequenced on an R9.4 MinION flowcell for 48 hours.