title: Nasal 16S rRNA gene amplicons obtained from healthy and BRD-positive pen-mates Holstein steers.
design: Nasal swabs were collected from aparently healthy (n=75) and BRD-affected (n=58) pen-mate Holstein steers. Nasal swabs were collected from July-December 2020. DNA was then extracted from the nasal swabs and then, the extracted DNA was used to construct the 16S rRNA gene library (V4 16S rRNA gene) following the protocol from the Schloss lab. The amplified DNA was normalized using the SequalPrep Normalization Plate (Invitrogen) and pooled to form a single library. The 16S rRNA gene amplicons were sequenced using Ilumina MiSeq (2x250, paired-end).