title: GSM6310735: Wildtype1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted by using Trizol reagent. Total 2ug RNA for the construction of sequencing libraries mRNA were enriched by Oligo(dT) bread and then fragmented by fragmentation buffer. cDNA was synthesized by reverse transcription using 6bp random primers and purified by QIAQuick PCR kit. After adapter ligation, DNA was PCR amplified sequences and band isolated from an agarose gel.