title: Duplex sequencing of Homo sapiens: adult male semen
design: DNA was fragmented to an average size of 350 bp. A unique molecular identifier was attached to both strands of a DNA duplex on both ends. A panel of probes (TwinStrand Human Mutagenesis Panel (Human-50 v1.0)) was used to isolate 20 2.4 kb-regions in the genome (a total of 50 kb) by hybrid selection, which were then amplified by PCR.