title: GSM6675118: root nodules, MG112 Non-Infected, rep 3; Lotus japonicus; RNA-Seq
library_construction_protocol: Six biological replicates were collected for each accession (Infected: MG-70, MG-79, MG-136; Non-infected: MG-9, MG-112, MG-115). Each biological replicate comprised 20 nodules from at least 5 different plants. Nodules were harvested 5 wpi. Total RNA was extracted using the SpectrumTM Plant Total RNA kit (Sigma-Aldrich) according to the manufacturer's instructions and treated with DNAse I (Ambion) prime-seq RNA-sequencing poly A enrichment, three prime counting