title: GSM7047273: MBH_P90_DES 12_3; Rattus norvegicus; RNA-Seq
library_construction_protocol: At PND22, PND42 and PND90,  the pre-optic area (POA) and the mediobasal hypothalamus (MBH) were dissected and frozen. Total RNA was extracted from the MBH and POA using Universal RNA mini kit (Qiagen,Venlo, Netherlands) following the manufacturer's instructions. Libraries were prepared using Truseq® stranded mRNA Sample Preparation Kits (Illumina, CA, USA) following manufacturer's instructions. Libraries were validated using QIAxcel Advanced System and quantified by qPCR using the KAPA library quantification kit. Libraries were multiplexed and sequenced on an Illumina NovaSeq6000 sequencer to generate more than ~25,000,000 paired-end reads (2 × 150 bases) per library.