title: 16SrRNA sequence of human saliva
design: Library Preparation for Sequencing and Sequencing Libraries are prepared according to Illuminas protocol for Preparing Libraries for Sequencing on the MiSeq (part# 15039740 Rev. D) for 2nM or 4nM libraries. If the library concentration is below 1nM an alternative method is used for denaturation. (2).  The components of the final load are listed below.  PhiX and genomes are added in 16s amplicon sequencing to create diversity.   Sequencing reagents are prepared according to the Schloss SOP, custom read 1, read 2 and index primers are added to the reagent cartridge.  FASTQ files are generated for paired end reads.