title: Illumina HiSeq 2000 paired end sequencing; EZH2-mediated H3K27me3 targets transcriptional circuits of neuronal differentiation
design: EZH2-mediated H3K27me3 targets transcriptional circuits of neuronal differentiation
library_construction_protocol: For every time-point, cells were scraped and lysed directly in RLT buffer and frozen at -80Â°C Total RNA was subsequently extracted using the RNeasy Mini Kit from Qiagen. Purified RNA was quantified using a NanoDrop spectrophotometer and RNA quality was checked with an Agilent 2100 Bioanalyzer using the RNA nano kit. Library preparation for RNA sequencing was performed according to the TruSeq RNA Library Preparation Kit. cDNA library quality was assessed at on Agilent 2100 Bioanalyzer, using the high sensitivity DNA kit.