title: The 16S rDNA sequence of microbial community from leachate
design: Water samples were filtered with vacuum suction filtration to collect the deposit on the milli-pore membrane and stored at -20 C for microbial community nalysis. Bacterial genomic DNA was extracted using a FastDNA Spin Kit. Amplified the V3-V4 region of the16S rRNA gene and sequenced on an Illumina MiSeq platform.