title: GSM5640766: complement_RNAseq_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted from two‐week old Arabidopsis seedlings using a RNeasy Plant Mini Kit (Qiagen, Germany). DNA in the isolated RNA were digested with TURBO DNase (Thermo Fisher Scientific, USA). Purified RNA was subsequently turned into cDNA as manufacturer's instructions with iScript™ cDNA Synthesis Kit (Bio-Rad, USA). RNA-seq libraries were prepared using NEXTFLEX® Rapid Directional RNA-seq Library Prep Kit (NOVA-5138-08)   RNA-seq libraries were pooled into one flow cell (NextSeq 500/550 High Output Kit v2.5) for sequencing with single-indexed, PE (2*75 bp) and sequenced on an Illumina Nextseq 500.