title: 16S metabarcoding of tomato rhizospheres
design: DNA from ~0.25g of rhizosphere soil was extracted with Qiagen DNeasy HTP 96 kits and sent to UMGC for library preparation and sequencing of the V4 region of the 16S rRNA, according to the methods outlined in Gohl et al. (2016). Sequencing was performed on the Illumina MiSeq with V3 chemistry and paired end 300 bp sequencing.