title: RNA-Seq of Veratrum maackii Regel: The leaf of a seedling
design: 1) The mRNA of veratrol samples was enriched by magnetic beads with Oligo (dT); (2) The enriched sample mRNA was randomly interrupted by FragmentationBuffer; (3) Fragment mRNA was used as template, and the first cDNA chain was synthesized using random primers, using RNaseH  DNA polymerase I to synthesize a second cDNA strand.  (4) The end of double-stranded cDNA was repaired, and a single adenine nucleotide was added to the end and connected for sequencing  Joint;  (5) Sample cDNA library was obtained by PCR amplification and enrichment, and AMPureXPbeads were used Systematic purification; (6) After the construction of sample library, Agilent2100 was used to detect the integrity of RNA, so as to ensure the quality of sample library.