title: GSM6722215: Airway organoid, Normal, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: The airway organoid with DNAH5 mutation and normal airway organoid pellets, derived from the biopsy, were resuspended with 1 mL of TrypLE 1× (Gibco, A1217701), separately, incubated for 15-30 min at 37 °C, and mechanically sheared with 1-ml pipettes. After washing twice with AdDF+++, the cell pellets were filtered with a 40-μm strainer. Cellular suspensions (∼6000 cells) were loaded on a Chromium Single Cell Instrument (10X Genomics) to generate single-cell GEMs. Sample processing and single-cell RNA-seq library preparation were performed using Chromium Single Cell 3′ v2 reagents. Sequencing was performed on an Illumina NextSeq500 following the manufacturer's instructions. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).