title: GSM6368916: WT, untreated, PRO-seq; Mus musculus; OTHER
library_construction_protocol: Nuclear run-on assays were performed essentially as described in Reimer et al. [K. A. Reimer, C. A. Mimoso, K. Adelman, K. M. Neugebauer, Molecular Cell (2021)] with modifications noted: 2X nuclear run-on buffer consisted of (10 mM Tris (pH 8), 10 mM MgCl2, 1 mM DTT, 300mM KCl, 20uM/ea biotin-11-NTPs (Perkin Elmer), 0.8U/uL SuperaseIN (Thermo), 1% sarkosyl). Library construction was performed as described in Reimer et al. [K. A. Reimer, C. A. Mimoso, K. Adelman, K. M. Neugebauer, Molecular Cell (2021)] PRO-seq