title: Bulk RNA-seq of homo sapiens T cells
design: Sequence reads were trimmed for adaptor sequences and masked for low-complexity or low-quality sequences. The number of raw reads mapped to genes was calculated by RSEM (rsem-1.2.4), and the sample results were combined and normalized by EDAseq (1.99.2). Gene expression fold-changes were calculated using normalized raw reads. The downstream analysis used glbase scripts.