title: Genome resequencing of Spodoptera frugiperda: Sf9
design: NGS established by PCR-based approaches for T-DNA site identification was used. All short reads were mapped to the reference genome of Spodoptera frugiperda and the sequence of the plasmid pIC-eGFP-attP. The reads were divided into three subgroups: 1) reads mapping to the reference genome only, 2) reads mapping to the plasmid sequence only, and 3) reads mapping to both the reference genome and the plasmid sequence. Reads mapping to both the reference genome and plasmid sequence were used to identify the insertion sites of landing pads.