title: GSM6675553: CRC-SC#1 shNT replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Extraction of total RNA from CRC-SC#1 cells was performed using the miRNeasy® Mini Kit (Cat No./ID: 217004 QIAGEN, Venlo, The Netherlands), according to the manufacturer's instructions. 500ng of high quality RNA (RIN>9) was used to prepare RNA-libraries using TruSeq Stranded Total RNA Kit (Illumina) according to manufacturer's instructions