title: 16S amplicons of Canada Goose fecal microbiome
design: 16S V4 rRNA DNA was amplified using PCR. Amplicons were sent off for library prep and sequencing.PCR products were combined using a QIAgility robot for liquid handling after the products were normalized based on the concentration of DNA from 350-420 bp. The pooled PCR products were cleaned using the Mag-Bind RxnPure Plus (Omega Bio-tek) according to the manufacturer's protocol. The cleaned pool was sequenced on the Illumina MiSeq using v.2 2_250 base pair kit.