title: GSM6722270: Regen, Lmx1bCreERT2TdT, 14 DPA, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was dissected from the regenerating digit tip at 14DPA. Freshly dissected tissue was digested in Liberase (280ug/mL) at 37°C for 1 - 4hr, with constant aggitation. Cells were mechanically dissociated with a small-bore-hole glass pipette, filtered through a 20um cell strainer and pelleted by centrifugation at 1000 rpm for 5 min. The cell pellet was then washed in 1X HBSS and cells were counted with a haemocytometer and pelleted again by centrifugation at 1000 rpm for 5 min. The cell pellet was re-suspended in a solution containing 0.04% BSA in 1X HBSS at a concentration of 500-700 cells/ul, prior to collection of single cell transcriptomes. Libraries were prepared according to the manufacturer's instructions (10X Genomics) using the Chromium Single Cell 3' Library and Gel Bead Kit.