title: WGS of Debaryomyces hansenii: KCTC27743
design: High-quality DNA was obtained from yeast spheroplast treated 0.5KU of zymolase (MP Biomedicals, 100T) using the spooling method. Pacific BioScience long-read sequencing data for D. hansenii KCTC27743 were submitted to the Sequel (Pacific Biosciences, USA) platform. To construct libraries for sequencing, more than 10 g of high-quality/high molecular weight genomic DNA was sheared up to 20 kbp using a 26g blunt needle (SAI Infusion Technologies, USA). Sheared genomic DNA was treated for damage repair and end repair, adaptor ligation, and size selection with a Blue Pippin system (Sage Science, USA), generating SMRTbell template libraries.