title: WGS of Escherichia coli: Wastewaters
design: The whole genome sequencing library was prepared using QIAseq FX DNA Library Kit for Illumina (Catalog: 180479, QIAGEN). In brief, 150ng of intact DNA was enzymatically fragmented by targeting 250bp to 450bp fragments size using a fragmentation reagent. The fragmented DNA is directly end repaired and an A is added to the 3 ends during the FX reaction, making the DNA fragments ready for adapter ligation. Following this step, platform-specific adapters are ligated to both ends of the DNA fragments. These adapters contain sequences essential for binding dual-barcoded libraries to a flow cell for sequencing.