title: RNA-seq of male Amaranthus tuberculatus: Mature flower tissue
design: Immediately after collection, all tissues were frozen in liquid nitrogen and stored at -80 C until RNA extraction. RNA was extracted using a Trizol-based method [136] with a DNase I treatment following extraction. Samples were checked for quality and quantity by running them on a Qubit analyzer, a 1% agarose gel, and a bioanalyzer. Samples were sent to the Roy J. Carver Biotechnology Center at the University of Illinois, Urbana-Champaign, for Illumina library construction and sequencing. Libraries were prepared using the Illumina TruSeq Stranded mRNAseq Sample Prep kit (Illumina Inc., San Diego, CA). 150bp pair reads were sequenced using a NovaSeq 6000 system using one NovaSeq S4 flow cell