title: GSM6674687: INP_Ath_DMSO1; Homo sapiens; OTHER
library_construction_protocol: RNA was quantified using the Agilent 2100 Bioanalyzer on an RNA 6000 Pico Bioanalyzer chip (Agilent). For deep sequencing analysis, library preparation was performed using the SMARTER Stranded Total RNA Seq kit v2-Pico Input Mammalian kit (Takara Bio), following the manufacturer's instructions. The libraries were amplified for 16 cycles (IP) or 12 cycles (input) of PCR.  Library size and distribution were checked using the Agilent 2100 Bioanalyzer using the Agilent High-sensitivity DNA Kit (Agilent). Libraries were then sequenced using HiSeq2500 (Illumina) by Novogen (China), and a minimum of 20 million paired-end reads were obtained per sample.