title: SpCas9 MISER Slice 5 naive library
design: The MISER library is theoretically composed of all possible N- and C-terminal fragments, including both duplications and deletions. To isolate deletions in a particular size range, the MISER library was digested with BsaI, to excise the dCas9 gene from the vector backbone and run on an agarose gel. Various slices of the MISER library were individually gel extracted, ligated into expression vector pSAH063, and transformed into E. coli.