title: GSM6675004: donor 28 allergic EVs [Sample 23]; Homo sapiens; ncRNA-Seq
library_construction_protocol: Extracellular Vesicles (EVs) were seperated from human milk using differential centrifugation and sucrose density gradient seperation. EVs were pelleted and sotred in Qiazol until RNA isolation. Small RNA (< 200 nt) was isolated using the miRNeasy micro kit (Qiagen) according to manufacturer's instructions. Input quantity of sRNA for library preparation was based on equal volume of purified milk EVs and ranged between 10-150 ng of sRNA per sample. The Illumina® Truseq small RNA Sample Prep Kit was used to process the samples according to the kit-specific guidelines.