title: GSM6153691: D4.5 WT CD8, replicate 1, GRID-seq; Mus musculus; OTHER
library_construction_protocol: Spleens from P14 mice were homogenized, and 1x106 cells/mL in TCM were pulsed with 1 ng/ml LCMV GP33–41 peptide for 1 hour at 37°C. Cells were washed once with equal volume of warm TCM, then plated in a 96 well-plate at 5x104 cells/well. Cells were harvested 4.5 days later and dead cells removed using the Dead Cell Removal kit according to the manufacturer's protocol (Miltenyi Biotec, Catalog number: 130-010-101). CD8+ T cells were then enriched using the CD8a+ T Cell Isolation Kit. Cells were then cross-linked, nuclei isolated and GRID-seq libraries prepared as described previously (Li et al., 2017; Zhou et al., 2019). GRID-seq