title: GSM6153760: AG from female isotype treated 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from adrenal glands using RNA extraction kit (QIAGEN cat No. 7413). Both adrenals were crushed using 0,1 mm glass beads in a precellys homogenizer (Biospec cat No. 11079101) containing RLT buffer from the RNA extraction kit. Total RNAs were extracted in RNase-free water from adrenals and were analyzed in DNBseqTM sequencing plateform using DNBSEQ stranded mRNA library. Paired RNA-seq reads were aligned to the Ensembl 84 Mus musculus reference genome with hisat2 (version 2.2.1) (Kim et al., 2019).