title: GSM6152934: EC, brDura, naive, 3; Rattus norvegicus; RNA-Seq
library_construction_protocol: CD31+ endothelial cells were isolated from tissues by digestion with 0.4 U ml-1 Liberase TM and 60 U ml-1 of DNase1 subsequently FACS sorted. Total RNA was purified from sorted cells using the TRIzol protocol (Invitrogen) Library preparation was performed using the TruSeq RNA Sample Preparation Kit (Illumina, Cat. N°RS–122–2002) starting from 100-500ng of total RNA