title: EgrMYB2 DNA Library E1 15 PCR Cycles
design: Genomic DNA was extracted using the NucleoSpin Plant kit (Machery-Nagel). After sonication and end-repair, truncated illumina adapters were ligated to E. grandis sheared gDNA and used for DAP-seq analysis using EgrMYB1 (E1 and E2 libraries), EgrMYB2 (E1 and E2 libraries), EgrMYB122 (E1 library), EgrMYB135 (E1 and E2 libraries) and HaloTag (E1 and E2 libraries). DNA affinity purification, library amplification (introduction of full indexes), and library pooling were all carried out as previously described by Bartlett et al. (2017) with minor modifications.