title: GSM6153208: ESCC, L4-1; Homo sapiens; RNA-Seq
library_construction_protocol: The fresh tissue samples endoscopic biopsied from ESCC patients were rinsed with PBS on ice. The Gel Bead & Multiplex Kit and Chip Kit (10× Genomics, Pleasanton, CA, USA) was used to construct a chromium single-cell 3′-library according to the manufacturer's instructions. Libraries were sequenced on an Illumina HiSeq X Ten system, and mapped to the human reference genome (build GRCh38) using CellRanger-4.0.0 (10× Genomics)