title: GSM5640790: A_control; Mus musculus; RNA-Seq
library_construction_protocol: Mice hippocampus were isolated rapidly with cuting into pieces <0.5cm and were homogenized using a glass dounce tissue grinder (Sigma, #D8938). First, nuclei were isolated with EZ PREP buffer(Sigma, #NUC-101). The number of isolated nuclei was adjusted to 300000 nuclei/ml with suspension buffer and Ficoll PM-400 was added to the nuclei suspension buffer. Second, nuclei were loaded at a concentration of 200 nuclei per microliter. Third, the emulsion was kept on ice for 30 minutes to allow release of nuclear RNA. Single-cell suspensions were loaded to 10x Chromium to capture  single nuclei according to the manufacturer's instructions of 10X Genomics Chromium Single-Cell 3' kit (V3) .The following cDNA amplification and library construction steps were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run,150bp) by LC-Bio Technology co.ltd., (HangZhou,China) at a minimum depth of 20,000 reads per cell.