title: GSM5175846: RNA_NSD1KO2_rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq: Total RNA from 10^6 cells was purified by RNeasy plus mini kit (Qiagen, Cat.# 74134). ATAC-seq: 10^6 mESCs were collected and lysed by lysis buffer (10 mM Tris pH7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% NP-40) and separated into 50 µl with 50,000 cells. CUT&RUN/CUT&Tag: mESCs were harvested and washed with Wash buffer (20 mM HEPES pH7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1X Roche Complete Protease Inhibitor EDTA-Free tablet), 10 μl pre-washed Concanavalin A coated beads in binding buffer (20 mM HEPES pH7.9, 10 mM KCl, 1 mM CaCl2, and 1 mM MnCl2) were added per sample. RNA-seq and ATAC-seq libraries were prepared for sequencing using standard Illumina protocols. Other libraries preparation was described previously(Kaya-Okur et al., 2019a).