title: GSM6368915: WT, untreated, MPP8, irCLIP, replicate 2; Mus musculus; OTHER
library_construction_protocol: Whole-cell lysates were generated in CLIP lysis buffer (50 mM HEPES, 200 mM NaCl, 1 mM EDTA, 10% glycerol, 0.1% NP-40, 0.2% Triton X-100 and 0.5% N-lauroylsarcosine) and briefly sonicated using a probe-tip Branson sonicator to solubilize the chromatin. Library were constructed as previously described (Zarnegar et al., 2016). irCLIP-seq