title: GSM6674722: hTSC_1048_CoV_24h_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using the Nucleospin® (Macherey-Nagel, USA) kit. Total RNA was quantified using Qubit fluorometer (Thermo Fisher Scientific, USA) and quality controlled on an Agilent Tapestation (Agilent, Santa Clara, USA). RNA-seq libraries were prepared using the Ribo-Zero Plus rRNA Depletion kit (Illumina) followed by TruSeq Stranded Total RNA kit (Illumina) at the IGM Genomics Center at UC San Diego. Libraries were pooled and sequenced on NovaSeq 6000 S1 Flow Cell (Illumina) to an average depth of 23 million uniquely mapped reads.