title: GSM5971593: PigGeCKO, AFB1, Round1; Sus scrofa; OTHER
library_construction_protocol: Illumina sequencing of sgRNAs in the PigGeCKO cells. Briefly, the genomic DNA from PigGeCKO cells were extracted using a Blood & Cell Culture DNA Midi Kit (QIAGEN), respectively. The sgRNA-coding region was amplified by PCR using KAPA HiFi HotStart High-Fidelity DNA Polymerase (NEB). PCR products were purified.