title: GSM6153586: rnaseq_581_KCl_6hr; Homo sapiens; RNA-Seq
library_construction_protocol: Patients with children-oset schizophrenia (COS), unaffected family members, and unrelated controls were recruited into a longitudinal study by Dr Judith Rapoport at the NIMH [23689535, 25510512]. The Rapoport laboratory generously provided fibroblasts that were reprogrammed. ATAC-seq was performed using an established protocol [PMID:25559105] with minor modifications. Following cell lysis, iPSC-derived neuronal nuclei were stained with DAPI (Thermoscientific) and sorted using a FACSAria flow cytometer (BD Biosciences). 75,000 nuclei were used as input for each ATAC-seq reaction and the resulting libraries sequenced at the New York Genome Center as 50bp paired-end reads on a HiSeq 2500 (Illumina).    RNA was isolated from approximately density-matched plates (initially seeded with ~200,000 NPCs per well) using TRIzol (Thermo Fisher), according to the manufacturer's instructions. The RNA Integrity Number (RIN) was determined using an RNA Nano chip (Agilent Technologies) on the Agilent 2100 Bioanalyzer. All samples have high RIN (mean ± standard deviation: 9.53 ± 0.22). 500 ng of total RNA was used as input material for library preparation using the TruSeq Stranded Total RNA Sample Prep Kit (LT) (Illumina, USA) according to manufacturer's instructions. Individual libraries were indexed as recommended by Illumina.