title: H10 evolved (in propionate medium, 8 g/L) clone_264
design: Strains were cultivated in BHI liquid medium overnight (30 , 200 rpm). 20 l culture was transferred into CGXII minimal medium (30 , 200 rpm) for 16h. Then 20 l culture was transferred into CGXII minimal medium with 8 g/L propionate medium (30 , 200 rpm) for 32 hours or 120 hours for initial strains. Cells were pelleted and stored at - 80 . Genomic DNA was isolated using Wizard Genomic DNA Purification Kit. 100 ng of gDNA was sheared to 600 bp using the fragmentation enzyme in xGen DNA Lib Prep EZ kit (Integrated DNA Technologies, Inc) and size selected using SPRI beads (Beckman Coulter). The fragments were treated with end-repair, A- tailing, and ligation of Illumina compatible adapters (Integrated DNA Technologies, Inc) using the xGen DNA Lib Prep EZ kit. Bioanalyzer High Sensitivity DNA Kit (Agilent) and Qubit Fluorometers (ThermoFisher Scientific) were used to determine the concentration of the libraries. Libraries were sequenced on the Illumina Miseq.