title: WGS of homo sapiens : adult male
design: The genomic DNA was fragmented with Frag enzyme (MGI, Shenzhen, China) to DNA fragments between 100 and 1,000 bp suitable for PE150 sequencing according to the manufacturer's instructions (MGI FS DNA library prep set, cat No. 1,000,005,256). The fragmented DNA was further selected to be between 300 and 500 bp by DNA clean beads (MGI, Shenzhen, China). The selected DNA fragments were then repaired to obtain a blunt end and modified at the 3 end to get a dATP as a sticky end. The dTTP tailed adapter sequence was ligated to both ends of the DNA fragments. The ligation product was then amplified for 7 cycles and subjected to the following single-strand circularization process. The PCR product was heat-denatured together with a special molecule that was reverse-complemented to 1 special strand of the PCR product, and the single-strand molecule was ligated using DNA ligase. The remaining linear molecule was digested with the exonuclease, finally obtaining a single-strand circular DNA library. We sequenced the DNA library using DNBSEQ-T7 (DNBSEQ-T7, RRID:SCR_017981) with a PE read length of 150 bp.