title: Transcriptome of A. sydowii
design: The sequencing libraries were prepared by random fragmentation of cDNA sample, followed by 5and 3adapter ligation. Adapter-ligated fragments were amplified by PCR, purified by gels, and the size of PCR enrichment fragments was verified through the template size distribution obtained in an Agilent Technologies 2100 Bioanalyzer using a DNA 1,000 chip. Libraries were quantified using qPCR according to the Illumina qPCR quantification protocol guide. 2x150 bp paired-end sequencing was performed on the Illumina HiSeq-2500 platform. Samples were sequenced by Macrogen Company (Seoul, South Korea), resulting in a minimum of 60 million reads per sample.