title: ChIP-seq of Arabidopsis thaliana : 3~4 week old arial part
design: 3~4 g of 3 to 4 weeks old plant leaves were crosslinked with 1% formaldehyde for 20min under vacuum, quenched with 125 mM glycine and ground into fine powder in liquid nitrogen. Chromatins were isolated and sheared into 200- to 500-bp DNA fragments by sonication. The sonicated chromatin was immunoprecipitated with 5 g of anti-H2A.Z (Abcam, ab4174), anti-H1 (Agrisera, AS11 1801) with 40 l of Dynabeads Protein G (Invitrogen, 10003D) for 12 h at 4 C with rotation. The precipitated chromatin DNA was then purified by phenolchloroform-isoamyl alcohol extraction and recovered by ethanol precipitation. ChIP-seq libraries were constructed using NEBNext Ultra II DNA Library Prep Kit (NEB, E7103) following the manufacturers instructions. Constructed libraries were sequenced using Illumina NovaSeq 6000 and paired-end reads were obtained at Geninus (https://www.kr-geninus.com).