title: WGBS of oncorhynchus kisutch liver (6 months) growth hormone transgenic
design: Pools of two individuals were combined.  DNA was quantified with a Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies).  Whole genome shotgun sequencing libraries were generated by using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) with 250 ng of DNA and Adapters from NEB.  Libraries were then size selected using sparQ PureMag Beads (Quantabio).  The EZ DNA Methylation-Lightning Kit (Zymo Research) was used for bisulfite conversion.