title: 16S rRNA seq of Sceloporus occidentalis: gut
design: We extracted whole DNA from each gut or gut-content sample using the SurePrepTM Soil DNA Isolation Kit (Fisher BioReagents #BP2815-50) and assessed extraction quality via NanoDrop (MODEL DETAILS).  We then sent extracted DNA to the Microbial Analysis, Resources, and Services (MARS) center at the University of Connecticut for 16s DNA 2-step PCR and sequencing (Bacterial 16S v4).