title: GSM5510089: H1N1-2; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was isolated by trizol reagent (Vazyme, China) according to the manufacturer's instruction Ribosomal RNAs were further removed by using ribo-zero-magnetic-kit (Epicentre, USA). RNAs were incubated with RNase R (Epicentre, USA), then purified by using AmpureBeads (Beckman, USA). RNA-seq libraries were prepared by using TruSeq® RNA LT Sample Prep Kit v2 (Illumina, USA) and were sequenced on Illumina Hiseq 3000 platform