title: scRNA of Homo sapiens: AEH sample
design: The collected endometrial tissues were mechanically cut by scalpels as small as possible, followed by dissociating in 20 ml of endometrial digestion medium (2.5 U/ml Dispase II (Sigma, D4693), 2mg/ml collagenase V (Sigma, C-9263) and 10% FBS) with gentle shaking at 37 C for 1-2 h. Pipetted up and down every 20 min. Till most cells in single cell status, suspended the digestion and filtered the digested cells with 40m cell sieves and obtained single cell suspension. The single cell suspension was centrifuged at 800rpm for 3min and then lysed red blood cell. For scRNA-seq, the percentage of the live single cells (counted by Typan blue) was over 80% in total endometrial cells before manufacturing in the single-cell-A-Chip (10X Genomics). Then, the chip with endometrial cells was loaded in a 10X Chromium single-cell instrument (10X Genomics). The following barcoding, cDNA synthesis and library construction were followed by standard manufacturer`s instructions. The qualified libraries were applied to the Illumina NovaSeq6000 platform for PE150 sequencing.