title: GSM6674977: 231_Synchronized RNA-seq_double-thymidine_Nocodazole_ActinomycinD_time 2; Homo sapiens; RNA-Seq
library_construction_protocol: MDA-MB-231 cells were collected with trypsin digestion and washed with PBS, and RNA was extracted using TRIzol. After strand-specific library construction, Novaseq6000 was used for sequencing, and the raw data of each sample were more than 10 Gb.