title: GSM6153768: iBCG (IBCG)_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: After co-culture of PBMC with BCG, for 7 days in RPMI complete medium, high viability single-cell suspensions were obtained using the Dead Cell Remove Kit (Miltenyi Biotec), and cells were pooled and resuspended in PBS+0.04% BSA prior to sequencing [library construction protocol: Low Input]  Library was generated according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads to generate partitioned cells (GEMs, Gel beads-in-Emulsion). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscribed to cDNA, incorporating an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the appropriate size fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added. [chemistry]  SC5P-R2 with sample multiplexing