title: Genomic Characterization of Escherichia coli Strain of O8:H9-ST410 Clone
design: E. coli strain EC13655 was cultivated on sheep blood agar at 37C overnight prior to DNA isolation by Invitrogen Easy-DNATM kit (Invitrogen, San Diego, CA, USA). DNA concentration was quantified on a QubitTM 2.0 fluorometer using the dsDNA BR assay kit (Invitrogen, San Diego, CA, USA). The genomic DNA was prepared for Illumina pair-end sequencing as per the Illumina NexteraXT DNA Library Prep Guide Document # 15031942 v05 May 2019 following the protocol. The library was sequenced on Illumina MiSeq using MiSeq reagent kit v2 and 500 cycles with a standard flow cell.