title: WGS of Homo Spaiens: stool from MODIC patients
design: The microbial DNA from the fecal samples was extracted manually using the PSP Spin Stool DNA Kit (Stratec molecular, Berlin, Germany) as per the manufacturers protocol. Libraries for shotgun metagenomic sequencing were prepared using the Illumina Nextera Flex DNA library prep kit according to the manufacturers instructions. The DNA libraries were then sequenced with 150-nucleotide-long paired-end reads on an Illumina HiSeq 3000 platform (Illumina Inc., San Diego, CA, USA). Low-quality and adapter sequences from raw sequencing reads were filtered using Trimmomatic. After quality filtering, the human DNA contaminant sequences were discarded from all samples by filtering out the reads that mapped against the human reference genome (GRch38, downloaded from NCBI GenBank) using Bowtie2. The clean reads are uploaded to public repository.