title: RNA-Seq of 10 hpf Astyanax mexicanus surface fish embryos: Biological replicate 3
design: RNA sequencing was carried out at the I2BC High-throughput sequencing platform, France (https://www.i2bc.paris-saclay.fr/spip.php?article399). All RNA samples were checked with a Bioanalyzer RNA 6000 pico chip (Agilent technologies) and passed the quality threshold (RIN >9) prior to library preparation. Libraries were generated from purified total RNA using polyA selection (llumina TruSeq Stranded Protocol). Samples were sequenced for between 75 and 100 million reads (paired-end, 5135 bp) using the Next-Seq 500/550 High Output Kit v2 (75 cycles). Reads were trimmed using Cutadapt 1.15.