title: Targeted nanopore sequencing of BOK circRNA-derived amplicons (ES-2 cell line)
design: Total RNA from ES-2 cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BOK exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.