title: Whole-genome long-read sequencing of C. briggsae wild isolate ED3036
design: High molecular weight DNA was extracted using either the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), or by standard phenol/chloroform extraction. Worms were washed off of freshly starved plates and rinsed with M9 at least 3 times before the pellet was frozen at -20 to facilitate sample lysis before the extraction protocol was started. 10 kb sequencing libraries were prepared using the 1D Ligation Sequencing Kit from Oxford Nanopore Technologies (ONT; SQK-LSK109) and sequenced on a PromethION device (ONT). Libraries for C. briggsae strains ED3036, JU439 and QR24 were barcoded using the Native Barcoding kit (#EXP-NBD104) and sequenced together. Read calling and demultiplexing (where applicable) was performed with the GPU-version of Guppy Basecalling software v5.0.11+2b6dbffa5 and the basecalling model dna_r9.4.1_450bps_sup_prom.cfg.