title: Actinobacterial diversity of ocean sediment based on 16S rRNA gene amplification: sample 4
design: For each site, six sediment samples were collected, each spaced 3m apart, giving a final number of 18 samples. Metagenomic DNA (mgDNA) was isolated (from 0.1g of each sample) using the DNeasy PowerSoil DNA Isolation Kit (Qiagen), according to the manufacturers instructions. Metagenomic DNA was extracted using the Powersoil kit and subjected to 16S PCR. Amplicons generated were submitted for Iontorrent Sequencing