title: AcpM-treated-2_miR
design: For small RNA-sequencing, the construction of the library was performed using NEBNext Multiplex Small RNA Library Prep kit (New England BioLabs, MA, USA) according to the manufacturers instructions. Briefly, for library construction, total RNA from each sample was used 1ug to ligate the adaptors, and then cDNA was synthesized using reverse-transcriptase with adaptor-specific primers. PCR was performed for library amplification, and libraries were cleaned up using QIAquick PCR Purification Kit (Qiagen) and AMPure XP beads (Beckman Coulter, CA, USA). The Agilent 2100 Bioanalyzer instrument assessed the yield and size distribution of the small RNA libraries for the High-sensitivity DNA Assay (Agilent Technologies). The NextSeq500 system produced High-throughput sequences to single-end 75 sequencings (Illumina).