title: RNA-seq of LDEV
design: 10 ng RNAs were then submitted to generate the sequencing library by SMARTer smRNA-Seq kit following the recommendations of the manufacturer. Sequencing libraries were amplified by 17 cycles of RCR to obtain DNA fragments corresponding to 140-200 bp. The prepared libraries were sequenced on a Hiseq platform (Illumina, San Diego, CA, USA) for 50 bp single-end reading.