title: Whole genome sequencing of bacterial isolates
design: Genomic DNA was extracted from fresh colony growth using DSP QIAamp DNA mini kits (Qiagen, Australia) on the QiaSymphony SP and quantified by fluorometry (Quant-iT dsDNA Assay High Sensitivity Kit; Life Technologies). Extracted DNA of a concentration greater than 1ng/L was acceptable for further processing. Paired-end DNA libraries were prepared using Nextera XT library prep kits (Illumina; Australia) and WGS was preformed using the Illumina NextSeq 500 (150 bp paired-end).