title: 103_ESP10-H&G
design: High-throughput sequencing of V4 16S rRNA (primers 515F and 806R) was used in all the samples, DNA obtained using the DNeasy Blood and Tissue kit  and library preparation performed in a two-step polymerase chain reaction (PCR) procedure. The PCR products from both amplifications were analyzed on agarose gels (1.5%), and DNA concentrations did quantify on a Qubit dsDNA HS and BR Assay Kit . Samples pool to equimolar concentrations