title: ITS amplicon sequencing of stem metagenome: pitaya stem
design: The primer pair ITS3 (5'- GCATCGATGAAGAACGCAGC -3') and ITS4 (5'- TCCTCCGCTTATTGATATGC -3') were used to amplify the fungal ITS sequences. PCR reactions were performed, and PCR amplicons were purified, pooled, and paired-end sequenced using an Illumina HiSeq platform.