title: Participant 3 rhabodomyosarcoma circulating tumor DNA whole geneome sequencing, timepoint 1
design: Cell free DNA was extracted from plasma using QIAamp Circulating Nucleic Acid kit (Kit #55114, Qiagen). Sequencing libraries were constructed from cfDNA (5-15 ng) using TruSeq Nano (Kit #20015965, Illumina) per the manufacturers' instructions with IDT UD Indexes v2 (Kit # 20040870, Illumina). Constructed libraries were balanced, pooled and sequenced using 150 bp paired-end reads on a Illumina NovaSeq 6000.