title: Nanopore metagenome of methanogenic enrichment culture AM
design: The library was prepared with 60 ng genomic DNA using the ligation sequencing gDNA kit (ONT) following the manufacturers instructions and minor modifications. In short, DNA repair and end-prep were performed using the NEBNext FFPE DNA Repair Mix and NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs), followed by the adapter ligation using Quick T4 ligase (New England Biolabs). Subsequently, a clean-up using AMPure beads (Beckman Coulter) and the short fragment buffer (SFB) was performed to retain DNA fragments of all sizes with a final incubation at 37 C for 10 min. The library was then directly loaded onto a primed SpotON R9.4.1 flow cell in a MinION Mk1C (1366 pores available). Sequencing was carried out for 44 h resulting in 1.57 M reads and 2.73 Gb raw data.