title: 24 hour
design: Method: Asian seabass Head kidney total RNA was extracted using the standard protocol, and its purity and quality were carefully evaluated using the bioanalyzer. Before proceeding with the assembly, the fastq files underwent pre-processing steps. This involved removing adapters and trimming the sequences based on a quality cut off of Q30, which was accomplished using the AdapterRemoval-V2 tool. Additionally, rRNA sequences were eliminated by aligning the reads with the SILVA database, ensuring that the subsequent analysis would focus on the non-rRNA transcriptome