title: Small RNA-Seq analysis of Ustilago Maydis
design: The solopathogenic strain SG200 of U. maydis has been described previously (Kmper et al., 2006). Total RNA was extracted from 4 ml overnight culture of sporidia or filamentous cells of U. maydis AB33 or SG200 with TRIzol reagent (Thermo Fisher Scientific, Waltham, MA U.S.A.) following manufacturers protocol. Small RNA-seq library was prepared using NEXTFLEX Small RNA-Seq Kit v3 (Perkin Elmer, MA, U.S.A.). Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatic (0.39)