title: 16S rDNA metabarcoding analyses from consortia obtained from goat digestive tract samples
design: Genomic DNA was extracted using MoBio DNeasy Powerlyzer DNA isolation Kit (Qiagen, The Netherlands), following the manufacturers instructions. DNA concentration and quality were determined using Nanodrop (Thermo Scientific) and by a 1% agarose gel electrophoresis, respectively. Genomic DNA was stored at -20C, until further analysis. Ten genomic DNA samples were sequenced at the Genoinseq Laboratory(Cantanhede, Portugal, https://www.cnc.uc.pt/en/services) in an Illumina Miseq V2 platform.The bacterial and archaeal structural diversity was determined by metabarcoding sequencing thehypervariable regions V3-V4 and V4-V5, respectively, of the metagenomic 16S rRNA gene with theset of primers 357wF 5 CCTACGGGNGGCWGCAG -3 and 926wR 5CCGTCAATTYMTTTRAGTTT -3 for domain Bacteria (Cilieborg, Boye, Thymann, Jensen, &amp;Sangild, 2011; Herlemann et al., 2011) and 517F 5 GCYTAAAGSRNCCGTAGC -3 and 909R 5TTTCAGYCTTGCGRCCGTAC -3 for domain Archaea (Baker, Smith, &amp; Cowan, 2003; Burggraf,Huber, &amp; Stetter, 1997).