title: RNA_seq of lettuce leaf
design: The mRNA from the total RNA samples was enriched using oligo magnetic adsorption, and the resulting RNA was fragmented. The RNA fragments served as a template for first-strand cDNA synthesis using random hexamers and reverse transcriptase. Second-strand cDNA was synthesized using DNA polymerase I and RNaseH and purified using a QiaQuick PCR extraction kit. Finally, cDNA fragments of a suitable length (300500 bp) were obtained by agarose gel electrophoresis and amplified by PCR to construct the final cDNA libraries for paired-end sequencing using the Illumina HiSeq 2500 system (Biomarker Technologies Co., Ltd, Beijing, China).