title: 16S rRNA gene sequencing of forest soil DNA: sample DFO3-1
design: Libraries for sequencing were prepared according to Illuminas 16S Metagenomic Sequencing Library Preparation guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (amplicon PCR). The template specific primers were (without the overhang adapter sequence): 515F-5 (5- GTGYCAGCMGCCGCGGTAA-3) and 926R (5- CCGYCAATTYMTTTRAGTTT-3).