title: RNA-seq of homo sapiens: SMAD4 knockout MCF7 cells treated with OHT
design: Total RNA was extracted with TRIzol (Invitrogen) according to the manufacturers instructions. The integrity and concentration of RNA were measured using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, USA). A total of 2 g RNA per sample was used and the sequencing libraries were generated with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7775). Sequencing was performed on an Illumina platform (Novogene Technology Co., Ltd., China) and 150 bp paired-end reads were generated.