title: Human PWB MSC 4221_3, derived from iPSC 4221_3, RNA-seq
design: RNA-seq libraries were constructed using a Qiagen Standard RNA Library kit as instructed by the manufactures manual. Paired-end sequencing was performed on a NovaSeq 6000 system (Illumina, San Diego, CA, USA) for 25-50 M reads per library. For RNA-seq, the raw reads were aligned and mapped to human reference genome (Homo_sapiens.GRCh38.dna.primary_assembly.fa.gz; Homo_sapiens.GRCh38.105.gtf.gz) using STAR. Validation and quantification of RNA transcripts were performed using FeatureCounts.