title: RNA-Seq of FReMV
design: Total nucleic acid was extracted from the plants using the Qiagen RNeasy Kit (Qiagen).  Total RNA from the samples was used to prepare RNA-Seq library using the TruSeq stranded Total RNA Sample Preparation kit with Ribozero Plant (Illumina, San Diego, CA) and the libraries sequenced using Illumina Novaseq with a paired-end read length of 2X151.  The resulting high throughput sequencing reads assembled and mapped using Geneious Prime v2023.1.1 to the target viruses obtained from GenBank (NCBI).