title: Whole-genome sequencing of C. briggsae HK104->AF16 NIL2
design: We extracted genomic DNA (gDNA) from at least two freshly starved 9 cm plates of the appropriate worm strain using the MasterPure Complete DNA and RNA Purification kit (Lucigen; cat. #MC85200), following manufacturer protocols, including the sample freeze/thaw step to facilitate tissue lysis. We then prepared Illumina sequencing libraries using the Nextera DNA Flex Library Prep kit (Illumina; cat. #20018705) with 100 ng of input gDNA for each library. gDNA and prepared libraries were quantified using the DeNovix dsDNA High Sensitivity kit (Biozym; cat. #31DSDNA-HI1), libraries were checked on a Fragment Analyzer and then sequenced on an Illumina NextSeq or MiSeq instruments.