title: Nanopore whole-genome sequencing of Caenorhabditis briggsae, wild isolate HK104
design: High molecular weight DNA was extracted by standard phenol/chloroform extraction. Worms were washed off of freshly starved plates and rinsed with M9 at least 3 times before the pellet was frozen at -20 to facilitate sample lysis before the extraction protocol was started. 10 kb sequencing library was prepared using the 1D Ligation Sequencing Kit from Oxford Nanopore Technologies (ONT; SQK-LSK109) and sequenced on a PromethION device (ONT). Read calling was performed with the GPU-version of Guppy Basecalling software v5.0.11+2b6dbffa5 and the basecalling model dna_r9.4.1_450bps_sup_prom.cfg.