title: PATSeq study of hemp males and females
design: Hemp seedlings were grown in soil for 7 days, at which time RNA was isolated from leaves without sacrificing the plants. Plants were grown until maturity to permit sex identification. RNA was fragmented and poly(A) enriched, and the poly(A) RNA used for PATSeq library preparation as described in Ma et al. (Methods 2014 May 1;67(1):74-83). After single-end sequencing, reads were demultiplexed, oligo-dT tracts trimmed from the 5-ends, and 3 ends trimmed to a constant read length of 40 nts. Reads are antisense to the mRNA and correspond to the mRNA-poly(A) junction.