title: RNA-Seq of Cardiocondyla obscurior: Middle aged queen Gaster colony C_27
design: Examination of gene expression patterns in Cardiocondyla obscurior ant queens.Total RNA was extracted using the ReliaPrep kit (Promega). Spike-In RNA Variant Controls (SIRV-Set 3 Lexogen#05101 Lot 5746/001492) were spiked to a 2% fraction of the total RNA. Total RNA was amplified using single primer isothermal amplification (SPIA , Ovation RNA-seq System V2, Tecan) prior to cDNA generation. Genomics using Nextera XT sequencing on a NovaSeq 6000 platform. Reads were trimmed with fastpv.0.20.1 to a minimum length 70 and from Nextera adapters. cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina) and the SuperScript II Reverse Transcriptase (Thermo Fisher Scientific).