title: archaeal 16S amplicon seq of Everglades WCA 1 peat core 3 at 85cm depth
design: We targeted the V3 and V4 regions of the archaeal 16S rRNA gene using the Arch349F (5-GYGCASCAGKCGMGAAW-3)  and Arch806R (5-GGACTACVSGGGTATCTAAT-3). PCR cycles were conducted with Phusion HotStart II Polymerase (ThermoFisher Scientific, Waltham, MA, USA) in the following steps: 30 seconds initial denaturation at 98 C followed by 35 x 10 second cycles of denaturation at 98 C, annealing at 54 C for 30 seconds, extension at 72 C for 45 seconds; and finally, 10 minute elongation at 72 C.