title: 16S Inf1
design: Bacteria present on the initial soils were extracted using 1Kg of soil diluted in 1L of sterile H2Od and incubated overnight at constant agitation of 100 rpm at 4C. All the volumes obtained (350-450mL) were diluted to a final volume of 850mL. From here, 1 mL of supernatant was transferred to 1.5 mL Eppendorf, and centrifuged at max speed for 10 min at 4C, the pellets were used for DNA extraction using DNeasy PowerSoil Pro kit, following manufacturer`s instructions. Then the 16S rDNA V3-V4 region was amplified by PCR using 341F and 806R primers. Samples were sequenced using Illumina Novaseq PE 2x250bp reads.