title: MiSeq of Ambystoma mavortium skin microbiome
design: Two mock community samples were added to the library. Cross-contamination oligos and synthetic gene sequences were added to each sample, and samples were normalized. PCR was used to amplify the 16S rRNA V4 region and ITS1 region and add unique dual index combinations to each sample for bacterial and fungal metabarcoding, respectively. Magnetic bead cleanup was performed following PCR, and amplicon product was pooled. Each sample received two PCR replicates.