title: Microbiome of mus musculus: adult female stool group DSS day 5
design: Total bacterial DNA from the feces pool was extracted with GenElute Stool DNA Isolation Kit (Sigma-Aldrich, St. Louis, MO, USA) and the V3-V4 region of the 16S ribosomal RNA gene was sequenced to generated 250bp paired-end raw reads using the HiSeq platform (Illumina Inc., San Diego, CA, USA)