title: Direct RNA-seq of RVA RNA from infected cells treated with TRICi
design: Total RNA was extracted from RVA-infected MA104 cells, treated with 2-[(4- chloro-24,1,3-benzothiadiazole-5-yl)oxy]acetic acid during infection. Then, RNA was ligated to sequence-specific reverse-transcription adapters recognizing 3' RNA ends of positive and negative strands of each of the eleven genome segments, processed for nanopore sequencing (SQK-RNA002) and sequenced with an Oxford Nanopore Technology MinION device using FLO-MIN106D.