title: 16S rRNA gene sequencing of fecal samples from bGH and control mice at three ages
design: Sample libraries were prepared and analyzed by barcoded amplicon sequencing. Purified DNA was amplified on the hypervariable V4 region of the 16S rRNA gene through PCR with primers F515 (5`-GTGCCAGCMGCCGCGGTAA-3`) and R806 (5`-GGACTACHVGGGTWTCTAAT-3`). The resultant amplicons (250 bp) were sequenced using pair end, high-throughput sequencing with Illumina MiSeq (20,000 avg. sequence read depth) at the UC Davis Genomics Facility.