title: 16S rRNA of the biofilm on 1d-c
design: To sequence the V4 region of the bacterial 16S rDNA gene, two-step, Nextera barcoded PCR libraries using the locus specific primer pair 515F (5- GTGYCAGCMGCCGCGGTAA -3) and 806R (5- GGACTACNVGGGTWTCTAAT -3) with 20 PCR cycles for the first step and 10 PCR cycles for the second step were created.