title: nifH amplicons from seawater samples
design: The nifH2 and nifH1 primers (Zehr and McReynolds 1989) modified with Illumina overhang adapters were used to amplify ~ 359 bp region of the nifH gene. nifH amplicons were sequenced using Illumina Miseq platform with 250bp paired reads at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia). See  https://doi.org/10.1002/lol2.10058 for full details of methodology.