title: MinION sequencing of artificially contaminated pasteurized milk with a commensal E. coli strain (5 CFU/mL) and a STEC strain (50 CFU/mL) enriched at 37C in acriflavine-supplemented BPW, third replicate
design: Preparation of genomic DNA library was performed according to the manufacturer's recommendation using 1-2 g of input DNA with a ligation sequencing kit (SQK-LSK-109) and a native barcoding expansion kit (EXP-NBD-104 and EXP-NBD-114). Libraries were loaded onto a R9.4.1 flow cell (Oxford Nanopore Technologies) and sequenced using the MinION (Oxford Nanopore Technologies). Basecalling and demultiplexing was performed with guppy basecaller and guppy barcoder.