title: 16S Rhizosphere F16WDS2E Replicate 2
design: Root and associated soil samples were placed in 50mL Falcon tubes. For the DNA extractions, Falcon tubes were filled with minimum medium, shaken 10min at max speed at 4C. After this, the tubes were left to settle for 15 minutes to decant the organic matter, and 10mL of the supernatant were transferred to 15mL Falcon tubes and centrifuged at max speed during 15min at 4C. Then, supernatants were discarded, and the pellets were used for DNA extraction using DNeasy PowerSoil Pro kit, following manufacturer`s instructions. Then the 16S rDNA V3-V4 region was amplified by PCR using 341F and 806R primers. Samples were sequenced using Illumina Novaseq PE 2x250bp reads.