title: K18-hACE2_SARS-CoV-2_WT_1dpi_lungs
design: RNA was isolated from the lungs isolated from K18-hACE2 mice, infected with SARS-CoV-2 and their corresponding Mac1 deletion virus for 1 day using trizol method. Library preparation was performed by the University of Kansas Genome Sequencing core facility, using the NEB Next RNA Library kit (NEB) with indexing. RNA-seq was performed using an Illumina NextSeq2000 high-output system with a paired-end reads of 50bp each. RNAseq data quality was checked using FastQC analysis pipeline. Samples had a minimum of 16 million reads and a mean quality score (PF) >33.