title: UCE target enrichment of Yavnella zhg-th03, male (CASENT0842750) [raw reads]
design: DNA was extracted non-destructively using a DNeasy Blood & Tissue Kit (Qiagen Inc., Valencia, CA) with DNA eluted from spin columns using buffer AE at 56 degrees Celsius (Cruaud et al. 2019). Genomic concentrations were quantified for each sample with a Qubit 2.0 fluorometer (Life Technologies Inc., Carlsbad, CA). Phylogenomic data were generated using the UCE probe set hym-v2 (Branstetter et al. 2017), with libraries being prepared and target loci enriched using protocols similar to Branstetter et al. (2017) at RAPiD Genomics (Gainesville, FL). Raw reads were assembled with SPAdes v. 3.12.0 (Bankevich et al. 2013).