title: Whole genome sequencing of homo sapiens: peripheral blood mononuclear cells
design: PBMCs were isolated from 5-10ml peripheral blood of individuals by Ficoll-Hypaque density gradient centrifugation and stored at -20 for future use. The Genomic DNA was generated using the NEB Next_ Ultra DNA Library Prep Kit for Illumina_ (NEB, USA). NanoDrop 2000 (Thermo Scientific, Waltham, MA) spectrophotometer was used for purity check, followed by agarose gel electrophoresis for degradation and contamination monitoring. Total amount of DNA > 700ng was at least required for library preparation and sequencing. NEBNext Adaptor hybridization and electrophoresis were performed to select specified length of DNA fragment.  Specially designed PCR protocol was used to amplify the tagged DNA and add sequencing indexes. Then AMPure XP system (Beckman Coulter, Beverly, USA) was used for fragments purification. A cBot Cluster Generation System using HiSeq 2500 PE Cluster Kit (Illumina) was used for cluster generation. At last, the library sequencing was performed by Illumina Hiseq 2500 platform (Illumina Inc., San Diego, CA, USA). Paired-end reads of 150bp were generated.