title: RNA-seq of danio rerio : whole embryo
design: We used 100 ng total RNA from all subjects to prepare sequencing libraries with by using the TruSeq stranded total RNA sample preparation kit(Illumina, CA, USA) which combines RiboZero rRNA depletion with a stranded specific method similar to the dUDP method. Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer (Agilent, CA, USA). They were quantified with the KAPA library quantification kit (Kapa Biosystems, MA, USA) according to the manufacturers library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end (2150bp) using Illumina NovaSeq6000 platfrom.