title: Functional metagenomic library of pooled metagenomic DNA from fecal samples of MODIC patients
design: Metagenomic DNA from patients treated with 3 months of amoxicillin were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli hosts. These E. coli transformant libraries were then screened against antibiotics at selective concentrations, and the cloned insert DNA in surviving transformants were prepared for sequencing using NEXTFLEX Rapid DNA-Seq Kit 2.0 (PerkinElmer). The raw reads were quality-trimmed using Trimmomatic for each samples, and were assembled into contigs using metaSPAdes.