title: DNA-seq of chicken gut microbiome
design: Bacterial DNA was extracted using the AquaGenomic Kit and KAPA PureBeads. Genomic DNA was measured using a Qubit 3.0 Fluorometer with Qubit dsDNA HS Assay Kit. Bacterial DNA was amplified with V3V4 primer region of the bacterial 16S rRNA gene. The PCR product libraries were quantified and qualified by using High Sensitivity D1000 ScreenTape.