title: Shotgun metagenomic sequencing of homo sapien: stool
design: DNA extraction was carried out using the QIAamp PowerFecal DNA kit (Qiagen Inc, Carlsbad, CA). Triplicate shotgun metagenomic libraries were constructed for each stool sample (except for one pair of maternal samples, which was sequenced in duplicate) using the plexWell LP384 kit (seqWell Inc., Beverly, MA) following the manufacturers protocol. Shotgun metagenomic sequencing of the pooled libraries was performed on the NovaSeq 6000 (Illumina, San Diego, CA) at 2 x 150 bp read length by Novogene Inc. (Sacramento, CA).