title: LPS 2
design: A 459 bp fragment of the V3/V4 regions was amplified using the primer pair 341F/ 785R (5-TCGT CGGC AGCG TCAG ATGT GTAT AAGA GACA GCCT ACGG GNGG CWGCA-G3) / (5-GTCT CGTG GGCT CGGA GATG TGTA TAAG AGAC AGAC AGGA CTAC HVGG GTAT CTAA TCC3) (Klindworth et al., 2013). Library construction was performed following the IIlumina 16S rRNA Cod 15044223 RevB protocol.