title: 16S rRNA amplicon sequence: Transmission of AMR plasmid in housefly gut microbiota
design: Total bacterial genomic DNA extraction was performed using a TIANamp Bacteria DNA Kit(TIANGEN, CHINA), following the manufacturers instructions. The concentrations of extracted gDNA were measured by a Qubit 2.0(life, USA) to ensure that adequate amounts of high-quality genomic DNA had been extracted. 16S rRNA gene libraries were constructed by amplified the bacterial 16S rRNA gene V3-V8 hypervariable region and the purified amplicons from each reaction mixture were pooled in equimolar ratios based on their concentration. Sequencing was performed using the Illumina MiSeq system (Illumina MiSeq, USA), according to the manufacturers instructions.