title: HMW Genomic DNA sequencing of Uloborus diversus with PacBio HiFi: adult female
design: HMW genomic DNA was extracted from a single adult female using a modified protocol with the Nanobind Tissue Kit. Size selection was peformed using a Sage Science BluePippin with a 9kbp high-pass cut-off. A PacBiolibrary was prepared using the Express v2 kit according to the standard protocol for preparing HiFi sequencing libraries. The library was sequenced on a PacBio Sequel II 8M SMRT Cell using a 30 hour HiFi run mode and processed using SMRT Link v.9.0 software.