title: NGS of JCVI-Syn3B
design: JCVI-Syn3B pellet preserved in Zymo Research DNA/RNA Shield (#R1100) and gDNA extracted using Zymo Research Quick-DNA Miniprep Kit (#D3024), with a lysozyme pre-treatment. The Oxford Nanopore Technologies(ONT) Ligation Sequencing Kit V14 (#SQK-LSK114 was used for amplification-free long-read sequencing library construction. Library was sequenced primer-free using ONT R10.4.1 flow cells (#FLO-FLG114).