title: shotgun sequencing of anal human metagenome
design: DNA samples were subject to cleanup and then processed for shotgun metagenomics sequencing using the PCR-free KAPA HyperPLUS (Roche, France) library preparation kit. We performed 100 nt paired-end sequencing using an Illumina NovaSeq 6000 System. Quality control on metagenomics sequencing data, were performed with KneadData tools, which separate the human (host) and the non-human microbiome  reads for further QC-based filtering.