title: Illumina HiSeq 2500 sequencing: age dependent changes in Caenorhabditis elegans gut microbiome
design: synchronized populations of germ-free L1 worms were raised at 20C in separate vials containing the same compost and harvested at L4 stage. DNA was extracted using QIAGEN PowerSoil DNA isolation kit and 16S V4 region was sequenced.