title: Illumina HiSeq 2000 sequencing of sediment from Taketomi Island shallow submarine hydrothermal field 10-15cm layer
design: DNA from the TIV sample was extracted using the Power Max Soil DNA isolation kit (MoBio Labs) and purified with the Aurora system (Boreal Genomics). Metagenome libraries from the sample were prepared from 1 ng purified DNA using the Nextera XT DNA library kit and sequenced with the Illumina HiSeq 2000 instrument at the Uppsala SNP&SEQ Technology Platform, generating 49 Gb of 2x250 bp paired-end reads, as previously described by Zaremba-Niedzwiedzka et al. (2017).