title: Direct RNA sequencing of BE(2)-C shGFP
design: BE(2)-C cells were washed with PBS, and total RNA was extracted using the Trizol method. Poly(A) RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L). A Direct RNA sequencing library was then prepared using Oxford Nanopore technology (ONT) Direct RNA sequencing protocol, version direct-rna-sequencing-sqk-rna002-DRS_9080_v2_revS_14Aug2019-minion. In brief, 700 ng of poly(A) enriched RNA was used as an input to prepare the Direct RNA sequencing library