title: TP53-MPRA of TP53-KO MCF7 cells
design: MCF7 TP53-KO cells were transfected with a barcoded TP53 reporter plasmid library. 24 hours later, RNA was isolated and cDNA was synthesised using a barcode-specific primer. Barcoded cDNA was amplified by PCR and subjected to high-throughput sequencing.