title: Illumina HiSeq 2500 sequencing: age dependent changes in Caenorhabditis elegans gut microbiome
design: synchronized populations of germ-free L1 worms were raised at 20C in separate vials containing the same compost and harvested at Post-gravid stage. DNA was extracted using QIAGEN PowerSoil DNA isolation kit and 16S V4 region was sequenced.