title: V4 16S rRNA amplicon sequencing of meat
design: Samples were collected at the end of aging process and immediately stored in -80C until isolation. Meat metagenomics was isolated using the DNeasy PowerLyzer PowerSoil kit following the manufacturers instructions. Extracted DNA was used for the construction of a 16S rRNA gene library following a the wet-lab protocol from the Schloss lab. Briefly, Illumina indexed reads were created using PCR amplification of the V4 region of bacterial 16S rRNA gene. No PCR amplicons were observed in the negative control samples using water as the DNA template. Amplified DNA was normalized using the SequalPrep Normalization Plate (Invitrogen), and pooled into a single library for each 96-well plate.