title: RNA-Seq of mus musculus: adult ovary
design: RNA was extracted from ovaries from 5 month old mice in the proestrous phase of the oestrous cycle. Prior to sequencing the following was done at BGI: 1)mRNA enrichment and purification: rRNA depletion or Oligo dT Selection to enrich the mRNA2)RNA fragment and reverse transcription3)End repair, add A and adaptor ligation4)RCR amplification5)Single strand separation and cyclization6)DNA nanoball synthesis