title: NGS of human stool : adult female
design: The sequencing libraries is prepared according to the Illumina 16S Metagenomic Sequencing Library protocols. The input gDNA 2ng was PCR amplified with 5x reaction buffer, 1mM of dNTP mix, 500nM each of the universal F/R PCR primer, and Herculase II fusion DNA polymerase (Agilent Technologies, Santa Clara, CA). The universal primer pair with Illumina adapter overhang sequences used for the first amplifications were as follows: V3-F: 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3, V4-R: 5- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3.