title: Illumina genome sequencing of Cynodon dactylon
design: Genomic DNA was isolated from the frozen leave samples using the DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). The DNA quality and concentration were tested by 1% agarose gel electrophoresis and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, USA). Two paired-end libraries with short insert size of 270 bp and 500 bp were constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, USA) and sequenced on the Illumina HiSeq X Ten platform (Illumina, San Diego, USA)