title: MinION sequencing; Nicotiana benthamiana
design: A total of nine MinION flow cells were run to generate long nanopore data for the assembly yielding between 3 and 20Gbp per flow cell. Two flow cells were run without DNA fragmentation, two flow cells with fragmenting DNA to 40Kbp using a Digilab Hydroshear and molecules <25kbp removed using a Sage Scientific Blue Pippin 0.75% cassette, two flow cells with fragmenting DNA to 40Kbp using a Digilab Hydroshear and molecules <15kbp removed using a Sage Scientific Blue Pippin High Pass cassette and three flow cells fragmenting DNA to 20kbp using a Covaris G-tube. All libraries were constructed and sequenced using the Oxford Nanopore Technologies SQK-LSK109 library construction kit and FLO-106 flow cells according to the manufacturersâ€™ instructions.