title: RNA-Seq of P. camtschaticus C1 juveniles, whole body homogenate
design: RNAlater-preserved crabs were homogenized with silica beads, Total RNA was isolated with TRIzol Reagent for total RNA, and stranded mRNA-Seq libraries were constructed with the NuGen Universal Plus mRNA kit. Libraries were prepared for sequencing using TruSeq RNA sample prep kits (Illumina). Paired-end sequencing was conducted on two lanes of a Hi-Seq 4000 with 100-bp read length.