title: Fecal microbiota profiling in patients with immune-related colitis
design: Extracted DNA was sheared to a target size of 650 base pairs (bp) using a Covaris ultrasonicator. DNA was then prepared for sequencing using the Illumina TruSeq DNA library preparation kit and sequenced using the Illumina platform targeting approximately 10-20 million reads per sample with 100-bp paired-end reads