title: Fresh minced beef
design: Food samples were initially homogenized using a stomacher and then centrifuged to separate the microbes from the food. Subsequently, DNA was extracted from the microbial samples using the QIAamp BiOstic Bacteremia DNA Kit. The library was prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (Cat No. 7530) according to the manufacturers protocol. The sample quality was then assessed using NanoDrop, agarose gel electrophoresis and the Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA). For sequencing, Illumina Novaseq 6000 (Illumina, San Diego, CA, USA) with 150bp paired-end was performed.