title: 16S human stool
design: DNA was isolated with Qiagen DNeasy Blood and Tissue kits as described previously (5). Bacterial 16S sequences spanning variable region V4 were amplified by PCR with primers F515/R806 and sequenced by Illumina MiSeq at the BCM Alkek Center for Metagenomics and Microbiome Research. 16S sequence data were processed using the MiSeq pipeline for mothur using software version 1.38.124,25 and the MiSeq SOP pipeline (version November 2021)