title: DNAseq of swine slaughterhouse clean zone before cleaning and disinfection
design: 1. Precise quantification of genomic concentrations using the Qubit dsDNA HS Assay Kit to determine the addition of library constructionTotal amount of DNA.2. A library of about 500 bp inserted fragment length was prepared, and the initial total DNA was about 500 ng. Use the ElutionBuffer diluted DNA to 130  L and loaded into 0.5 mL Covaris DNA fragmentation tube.3. Fragment according to S220 operating parameters using Covaris instrument.4. Concentrate and recycle broken DNA fragments using 1 times Hieff NGS DNA Selection Beads. purificationQubit 4.26 was used for detection.