title: Whole genome resequencing of cluster bean (Cyamopsis tetragonoloba)- mutant
design: 500 ng  of  Qubit quantified DNA  was sheared  using  Covaris  S220  sonicator to generate specific fragments with size range of 200-250 bp. The fragments were purified using HiPrepcleanup beads.Purified fragments were end-repaired, adenylated and ligated  to  Illumina  multiplex  barcode  adaptors  as  per  NEXTflex  Rapid  DNA  sequencing bundle kit protocol. Adapter-ligated DNA was purified using HiPrep clean up beads. Resultant fragments were amplified for 4 cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid  DNA  sequencing  Bundle.  Final  PCR product  (sequencing  library) was purified  with HiPrep cleanup beads followed by library quality control. Sequencing library was quantified by  Qubit  fluorometer and  the  fragment  size distribution was analyzed on Agilent 2200 TapeStation.