title: JSNZ isolate
design: S. aureus mouse-adapted DIP used in this study was growth in tryptic soy agar (TSA). S. aureus cultures were grown overnight in 5mL tryptic soy broth (TSB) in 15 mL tubes shaking with a 45 angle at 37 C. The bacteria were subcultured in fresh TSB at 1:100 for 3 h at 37C with shaking. Bacteria were centrifuged at 4,000 rpm for 5 min and washed two times with PBS. The bacteria were normalized OD to 109 CFU/ml and diluted to the indicated inoculum in PBS for infection.