title: DNA metabarcoding of wheat endophytic microbiome: bacterial profiling with 799F/1193R primer sets with BL_V5V7_1L (rep 2)
design: Endophytic microbial DNA was extracted from wheat leaf tissues. PCR amplifications were then carried out to amplify a bacterial or fungal marker sequence in a metabarcoding approach. For some samples, a developed PCR clamp was added in the mix, with the aim of blocking host (wheat) DNA co-amplification. Amplicons were sequenced on a 2x300 bp MiSeq Illumina device.