title: CircRNA identification from HeLa cell lines
design: DNA-depleted total RNA was utilised for ribosomal depletion and RNase R treatment. The remaining putative circRNAs were converted into cDNA using H-minus reverse transcriptase followed by RNase H treatment. The cDNA so formed was subjected to Phi29 DNA polymerase mediated-multiple displacement amplification (MDA). The high molecular weight amplicon was quantified and subjected to optimal size fragmentation. Purified fragments were end-repaired, adenylated and ligated to Illumina adapters followed by PCR cycling. These amplified fragments were further purified, quantified and sequenced.