title: 16S rRNA amplicon Nanopore of Sitobion avenae Endosymbionts
design: Bacterial universal primers FD1 (AGAGTTTGATCCTGGCTCAG) and RP2 (ACGGCTACCTTGTTACGACTT), amplify total endosymbiont community. Amplicon libraries generated using Native Barcoding Kit 24 V14 (Oxford Nanopores) and sequenced on PromethION 24 instrument using a PRO-114M flowcell