title: CircRNA identification from O. sativa Indica leaf
design: DNA-depleted total RNA was utilised for ribosomal depletion and RNase R treatment. The remaining putative circRNAs were subjected to fragmentation and priming followed by two step cDNA synthesis. Double stranded cDNA was purified, end-repaired, adenylated and ligated to Illumina adapters followed by PCR cycling. These amplified fragments were further purified, quantified and sequenced.