title: HMW Genomic DNA sequencing of Uloborus diversus with ONT: adult female
design: HMW genomic DNA was extracted from a single adult female using the QIAgen MagAttract HMW DNA kit using the standard protocol for manual purification of HMW gDNA from fresh tissue. Sequencing libraries were prapred using the Ligation Sequencing Kit (SQK-LSK109) according to the standard protocol. Third party reagents we used during library preparation included: New England Biolabs NEBNext End Repair/dA-Tailing Module (E7546), NEBNext FFPE DNA Repair Mix (M6630), and NEB Quick Ligation Module (E6056). We then sequenced the libraries, using ONT R.9.4.1 flowcells (FLO-PRO002) on an ONT PromethION sequencing platform.