title: WGS PacBioHiFi reads Rhodococcus opacus DSM43205
design: A sample of R. opacus DSM43205 was sequenced by SeqCenter (USA). Genomic samples were fragmented using the Covaris g-TUBE following recommended PacBio procedures [1]. Sequencing libraries were prepared following the PacBio SMRTbell prep kit 3.0 with the SMRTbell barcoded adapter plate 3.0 [2]. Pooled libraries underwent the PacBio binding and cleanup steps following the Sequel II binding kit 3.2 [3] and were loaded onto the PacBio Sequel IIe for a 15hr collection period with options enabled to retain subreads and sequencing metrics. Demultiplexing, quality control, and adapter trimming was performedon instrument via Lima [4]