title: 16S rRNA metagenome from an intermittent freshwater creek
design: 500 ml of water filtered onto 3 _m filters. DNA was extracted with the DNeasy Powerbiofilm Isolation kit. DNA was quantified with a Qubit 2.0 Fluorometer. Illumina MiSeq (2x250 bp) was used to sequence the V4 region of the 16S rRNA gene with the primer pair 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GACTACVSGGGTATCTAAT-3) using a dual index approach.