title: Single-cell sequencing of  leiocassis longirostris skin infected bacterium
design: The indexed sequencing libraries were created utilizing the Chromium Single Cell 3' Library v3 Kit (10 Genomics) following the manufacturer's instructions, which included fragmentation, end repair, and A-tailing. The final single cell 3 library contained the standard Illumina paired-end constructs which begin and end with P5 and R1 primers. The barcoded sequencing libraries were quantified using a Bioanalyzer Agilent 2100 System (Agilent, Santa Clara, CA, USA) and quantitative PCR using a KAPA Library Quantification Kit (KAPA Biosystems). Finally, two libraries were sequenced on the HiSeq2500 (Illumina, San Diego, CA, USA) with a paired-end sequencing mode (26 base pairs for read 1 and 98 base pairs for read 2