title: RNA-Seq of Medicago truncatula:leaf under alkaline stress
design: RNA libraries were prepared using the TruSeqTM RNA sample preparation kit from Illumina (San Diego, CA, USA) using 1 g of RNA. Messenger RNA (mRNA) was enriched and randomly fragmented into small fragments of approximately 200 bp, and cDNA synthesized using a SuperScript double-stranded cDNA synthesis kit (Invitrogen, CA, USA). The synthesized cDNAs were subjected to end-repair, phosphorylation, and A base addition according to Illuminas library construction protocol. Libraries were size selected for 200300 bp cDNA target fragments using 2% Low Range Ultra Agarose electrophoresis followed by enrichment of PCR (sample preparation kit; Illumina, San Diego, CA). After quantification using TBS380 (Turner BioSystems, Sunnyvale, CA, USA), the paired-end RNA-seq sequencing library was constructed on an Illumina HiSeq xten/NovaSeq 6000 platform, and 150 bp paired-end reads were generated.