title: HaCaT cell line, KO TP53, replicate 2
design: For transcriptomic analysis HaCaT cells were seeded onto 100 mm Petri dishes (Corning, USA) at a density of 1.0106 cells per plate. The cells were cultured for 24 hours, then the culture medium was replaced with containing cadmium. After 24 hours, the cells were collected by trypsinization following RNA isolation. Total RNA was isolated according to the manufacturers protocol and quantified using NanoDrop-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Transcriptome data were obtained by high throughput paired-end sequencing using Illumina NovaSeq 6000 with a read length equal to 100 bp. The TruSeq Stranded mRNA Library Prep Kit was used to prepare RNA libraries. All steps were carried out in accordance with the manufacturers protocol. Transcriptome profiling was performed in three replicates with a separate process of RNA extraction for each replicate.