title: ZJUG0153-RNA3
design: Library preparation and sequencingONT Ultra-longFor each ultra-long Nanopore library, approximately 8-10 g of gDNA was sizeselected (>50 kb) with SageHLS HMW library system (Sage Science, USA), and processed using the Ligation sequencing 1D kit (SQK-LSK109, Oxford Nanopore Technologies, UK) according the manufacturers instructions. About 800ng DNAlibraries were constructed and sequenced on the Promethion (Oxford Nanopore Technologies, UK) at the Genome Center of Grandomics (Wuhan, China).