title: Fresh non-minced beef
design: Food samples were initially homogenized using a stomacher and then centrifuged to separate the microbes from the food. Subsequently, DNA was extracted from the microbial samples using the QIAamp BiOstic Bacteremia DNA Kit. Libraries were prepared using the Rapid Barcoding Kit (SQK-RBK110.96) from ONT according to the manufacturers protocol. Ten barcoded libraries were then pooled with equal concentrations. After adapter ligation, sequencing was performed using the Flow Cell (R9.4.1) with the GridION (Oxford Nanopore Technologies) for >24h.