title: 16S rRNA microbiome of Octopus vulgaris: Skin Male
design: 16S rRNA V4-  two steps PCR library preparation protocol for amplification and subsequent index addition (Illumina, 2013). Primers 515F (Parada et al., 2016); 806R (Apprill et al., 2015)) (1st PCR) and Dual indices and Illumina sequencing adapters were attached using the Nextera XT Index kit (2nd PCR). Sequences were obtained using an Illumina MiSeq (San Diego, CA) v3 paired-end 300 bp protocol for 600 cycles.