title: Amplicon sequencing from office building wastewater: primer set targeting Pepper Mild Mottle Virus
design: Reverse Transcription using SuperScript IV First Strand Synthesis System; PCR amplification using FastStart High Fidelity PCR System (Roche) using PMMoV primers from Rosario et al. Appl Environ Microbiol 75:7261-7267; Purification and normalization using SequalPrep Normalization Plate Kit (ThermoFisher); Sequencing using MiSeq Reagent Kit V2