title: 16s DNA of citrus rhizosphere soil microorganisms
design: PCR products were purified by AMPure XP Beads and quantified by Qubit3.0 after purification. The products of the second round of amplification were purified by AMPure XP Beads, quantified by ABI StepOnePlus Real-Time PCR System, and sequenced by Novaseq 6000 PE250 mode pooling on the computer