title: Hi-C genome sequencing of Cynodon dactylon
design: Fresh leaf samples were fixed in 1% formaldehyde to maintain the 3-D structure of genome. Genomic DNA was extracted and digested with restriction endonuclease MboI. The digested DNA fragments were biotin-labeled at the ends and ligated to each other randomly. The ligated DNA was sheared into 300-600 bp fragments, blunt-end repaired and purified using streptavidin pull-down. The purified DNA was also sequenced on the Illumina HiSeq X Ten platform