title: RNA-Seq of Tococa quadrialata: stem, ctrl 1
design: RNA was isolated from ground leaf powder utilizing the SpectrumTM Plant Total RNA Kit (50) (Sigma-Aldrich)  with an on-column DNA digestion using DNaseI (ZymoResearch). After a further purification step (OneStep PCR Inhibitor Removal Kit, ZymoResearch), RNA concentration, purity, and quality were assessed using a spectrophotometer (NanoDrop 2000c; Thermo Scientific) and an Agilent 2100 bioanalyzer (Agilent Technologies).The isolated RNA was sent to the Max Planck Genome Centre, Cologne, Germany. There, 25 M paired end reads of 150 bp per sample were generated from the transcriptome libraries of leaves and other organs of uncolonized plants that were subjected to herbivore or control treatment.