title: VDJ Sequencing of Human B Cells: plasmablasts
design: PBMCs collected 1 week after one dose of the H1 stabilized stem ferritin vaccine from participant P39 were stained with B cell markers and fluorescently labeled influenza H1 hemagglutinin and H1 stabilized stem.  Plasmablasts from three populations (H1 stem negative, H1 upper stem+, H1 lower stem+) were bulk sorted separately.  Each population was then stained with barcoded hashtags (Biolegend) and combined into one pool.  cDNA was made using the 10x Genomics Chromium Next GEM Single Cell 5 Kit v1 per the manufacturers instructions. V(D)J genes were then amplified, and libraries made for NGS sequencing using the v1 10X Genomics BCR amplification and library preparation kits according to the manufacturers instructions.