title: EC_W3110_NR
design: E.coli W3110, E.coli W3110-10, and E.coli W3110-95 were grown in 5 ml LB broth at 160 rpm for 18 hours. Then, the cells obtained from these precultures were transferred to 10 ml of fresh LB broth by performing a 1/100 dilution and incubating at 160 rpm for 18 hours. Cells were then harvested and genomic DNA isolation was performed according to the manufacturer's protocol using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). Sequencing library was prepared using Nextera XT DNA Library Preparation Kit Kiti (Illumina, San Diego, CA, USA) and sequencing was done by Illumina NovaSeq 6000 platform Kiti (Illumina, San Diego, CA, USA) as paired-end (PE) 2x150 bases reads.