title: pacbio seqencing data
design: SMRTbell libraries were constructed according to the standard protocol of PacBio using 15 kb preparation solutions (Pacific Biosciences, CA). The library preparation was mainly divided into the following five steps: 1) genomic DNA shearing; 2) DNA damage repair and A-tailing; 3) ligation with hairpin adapters; 4) nuclease treatment of SMRTbell library; 5) size selection and binding to polymerase.