title: Hot water
design: The DNA was extracted from water filtered on 0.2 micrometer polycarbonate filter, then DNA amplicons awere prepared using PCR with specific primers (black) targeting e.g. the 16S rRNA, gene variable regions for bacterial taxonomic marker genes. A second processing step adds sequencing adapters. Resulting amplicon libraries were DNA sequenced and then basecalled. Basecalled sequences were adapter trimmed,and quality filtered. DNA reads were partitioned into clusters (either OTUs or ASVs) providing the read abundance of each cluster. Read sequence taxonomy is assigned by searching against a reference database. OTU/ASV tables correlating taxonomy and abundance are generated for further results analysis.