title: PacBio genome sequencing of Cynodon dactylon
design: High molecular weight (HMW) DNA fragments were separated from the extracted genomic DNA samples using BluePippin Size Selection System (Sage Science, Beverly, USA) through pulse-field gel electrophoresis and eight 20-kb sequencing libraries were constructed using SMRTbell Template Prep Kit (Pacific Biosciences, Menlo Park, USA) following the manufacturers instructions. The libraries (16 SMRT cells) were sequenced on the PacBio RSII platform (Pacific Biosciences)