title: Long-read DNA-Seq of Marasmius tenuissimus MS-2
design: Mycelium of Marasmiellus tenuissimus isolate MS-2 was grinded in liquid nitrogen. Genomic DNA was extracted using the CTAB method. Library preparation was conducted with the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit V14 (Oxford Nanopore Technologies) with the NEBNext Companion Module for Oxford Nanopore Sequencing. Sequencing was conducted with an ONT MinION Flow Cell (R10.4.1) on the ONT MinION Mkc1 platform.