title: nvd2
design: 15 dpi root nodules were chopped and collected for RNA sequencing (RNA Seq) for R108, nvd1 and nvd2. Total RNA was isolated using QIAzol Lysis Reagent in accordance with the manufacturers protocol. RNA seq library was prepared using the Breath Adapter Directional sequencing (BrAD-seq) method (Tonsley et al., 2015). Strand- specific mRNA libraries were prepared using YourSeq Full Transcript RNA-seq Library Kit (Amaryllis Nucleics, Oakland, CA, USA) as per the manufacturers protocol. RNA-seq was performed using Illumina HiSeq platform.