title: 3' RNA-Seq of Tall Fescue (Festuca arundinacea) and epichloe endophyte: heat stress response
design: Four isogenic pairs with and without endophyte were generated and 2 replicates were used for each plant which result in 16 plants total. Heat stress assays were conducted in two growth chambers, 8 plants in each chamber (4 E+, 4E-). Plants were first kept in growth chambers at normal conditions (25C day 16C night) for a week to establish acclimatization. Following the acclimatization process, the condition of one of the chambers was brought to high temperature conditions (38C day / 30C night). The other chamber was kept at normal conditions (25C day 16C night). In order to control water stress, all plants in both chambers were watered daily. The leaf and tiller samples (2 per plant, bulk) for 3' mRNA sequencing were collected right before the heat treatment and at 1 h and 24 h after heat treatment. RNA-Seq libraries were prepared using the Lexogen QuantSeq 3 mRNA-Seq library prep kit for Illumina. Libraries were generated by a Illumina MiSeq run.