title: ChIP-Seq of Solanum lycopersicum: GLK1 Solyc07g053630 rep2
design: Fully expanded leaves were harvested and cross-linked with 1% (w/v) formaldehyde solution. Anti-GFP (#A-11122, ThermoFisher) and anti-HA (#C29F4, Cell Signaling Technology) antibodies were used for GLK ChIP experiments. The supernatants of sheared chromatins were incubated with specific antibody, and washed subsequently with low salt buffer (20 mM Tris-HCl pH8.0, 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS), high salt buffer (20 mM Tris-HCl pH8.0, 500 mM NaCl, 2 mM EDTA, 1% Triton X-100, 0.1% SDS), LiCl buffer (10 mM Tris-HCl pH8.0, 250 mM LiCl, 1% Triton X-100, 0.7% sodium deoxycholate, 1 mM EDTA) and TE buffer. The immunoprecipitated DNA was tagmented with prepared Tn5 enzyme mix and reverse cross-linked at 65C. Then the DNA was purified with a DNA Clean & Concentrator-5 column (Zymo Research) and PCR amplified to get the finial library