title: 16S rRNA gene V1-V3 sequencing of PCR non-template control (KAPA HiFi HotStart)
design: Amplification of 16S rRNA gene V1-V3 region using 12 phased primer pairs derived from 27Fmod: 5' AGRGTTTGATCMTGGCTCAG 3' and 519R: 5' GWATTACCGCGGCKGCTG 3' primers. 2nd Indexing PCR using Illumina Nextera v2 system