title: PacBio SMRT continuous long read genome sequencing of Acidovorax temperans strain LMJ.
design: Genomic DNA was isolated from Acidovorax temperans strain LMJ (colony # 10) using Qiagens blood and cell culture DNA mini kit. At the Georgia Genomics and Bioinformatics Core (University of Georgia, Athens, GA), the sample was processed for preparation of the PacBio Single Molecule Real Time (SMRT) bell sequencing library according to the protocol given in the PacBio technical manual for template preparation and sequencing. The SMRT bell sequencing library was barcoded and sequenced with two additional barcoded microbial  SMRT Bell sequencing libraries in a single SMRT cell using the PacBio SMRT Continuous Long Read sequencing on the PacBio Sequel II instrument.