title: 16S-S25-1-1
design: Bacterial and fungal community structure wereas evaluated for all samples by amplification of the 16S and Internal Transcribed Spacer (ITS) rRNA genes, respectively. For bacteria, the hypervariable V4 region of the 16S rRNA gene was amplified using the 515F and 806R primer set [Green et al. 2015; Moonsamy et al. 2013], and fungal primers ITS1F and ITS2R [Walters et al., 2015]. Each primer set also contained the Fluidigm adaptor primers CS1F and CS2R [Green et al. 2015].(PCR setting: 25 cycles of 10 sec at 95 Celsius, followed by 1 sec at 50 Celsius (for 16S) and 52Celsius (for ITS), followed by 1 sec at 68 Celisus).Amplicon sequencing was conducted using a paired end 2x250 bp cycle run on Illumina MiSeqV2 chemistry at Rush University Genomics and Microbiome Vore Facility