title: RNA-seq of A549 cell
design: Using fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA was synthesized in the M-MuLV reverse transcriptase system, and then the RNA strand was degraded by RNaseH, and dNTPs were used as the DNA polymerase I system. The starting material is used to synthesize the second strand of cDNA. The purified double-stranded cDNA is end-repaired, A-tailed, and connected to a sequencing adapter. The 250-300bp cDNA is screened with AMPure XP beads, PCR amplification is performed, and AMPure XP beads are used to purify the PCR product again, and the library is finally obtained.