title: 16S rRNA gene V4 amplicon sequencing of the bacterial communities of nasal samples of surgery patients with and without intranasal povidone-iodine
design: PCR amplification of the bacterial 16S rRNA gene V4 region was performed using custom primers (Kozich et al. 2013). Amplicons equimolarly pooled and sequenced on an Illumina MiSeq using 2x250bp paired-end sequencing.