title: A thaliana Wild type root   3
design: For RNA-seq data, AP and roots of 14-day-old plants vertically grown on 1/5 MS plates under eCO2 conditions were used. Three independent biological replicates of each sample were harvested at after 10 h in the light period for the analysis. Total RNA extraction, preparation of RNA-seq library and sequencing step was performed as in Anoman et al. (Anoman et al., 2019). Quality check, trimming, alignment, reads counting and filtering of lowly-expressed genes was conducted as in Sun et al. (Sun et al., 2018).  References; Anoman, A. D., Flores-Tornero, M., Benstein, R. M., Blau, S., Rosa-Tllez, S., Brutigam, A., Fernie, A. R., Muoz-Bertomeu, J., Schilasky, S., Meyer, A. J., et al. (2019). Deficiency in the phosphorylated pathway of serine biosynthesis perturbs sulfur assimilation. Plant Physiol 180:153170. Sun, X., Matus, J. T., Wong, D. C. J., Wang, Z., Chai, F., Zhang, L., Fang, T., Zhao, L., Wang, Y., Han, Y., et al. (2018). The GARP/MYB-related grape transcription factor AQUILO improves cold tolerance and promotes the accumulation of raffinose family oligosaccharides. J Exp Bot 69:17491764.