title: RNAseq A.thaliana
design: Total RNA was extracted from roots of plants 6 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS) libraries were  generated using the TruSeq Stranded mRNA library prep kits (Illumina, San Diego, CA, USA). Libraries were sequenced on a HiSeq2500 (Illumina, San Diego, CA, USA) instrument as single  read 50bases. NGS analysis was performed using Tophat2 for mapping reads onto the Arabidopsis genome (TAIR10)