CDKN1B-Seq of Homo sapiens: adult female bone marrow biopsy
Specific primers covering the complete ORF of human CDKN1B gene were designed and modified adding specific adapter sequences. Amplicon libraries were generated using a modified Illumina protocol: multiplex PCR products were generated using Phusion High-Fidelity DNA Polymerase and subsequently tagged with specific Index primers. Purified libraries were pooled, and paired-end sequenced.