GSM6820571: Renal cortex_CLP1 repeat 3; Mus musculus; RNA-Seq
Briefly, Total RNA from renal cortex sample was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. The quantity and quality of the RNA samples were determined with a NanoDrop spectrophotometer (Thermo Fisher Scientific, MA, USA). The RNA integrity was examined by 1.5% agarose gel electrophoresis and quantified with a QubitTM RNA Broad Range Assay kit (Q10210, Life Technologies). Total RNAs (2 μg) were used for stranded RNA sequencing library preparation by using KCTM Stranded mRNA Library Prep Kit for Illumina® (DR08402, Seqhealth Technology) following the manufacturer's instruction. Total RNAs (2 μg) were used for stranded RNA sequencing library preparation by using KCTM Stranded mRNA Library Prep Kit for Illumina® (DR08402, Seqhealth Technology) following the manufacturer's instruction.