GSM6560197: IgG-41-EFGF; Homo sapiens; RNA-Seq
RNA was extracted using RNEasy Mini kit with gDNA eliminators columns (QIAGEN). RNA-Seq experiment was performed with the starting material of 500 ng total RNA. The prepared libraries were quantified with the KAPA Library Quantification Kit (KK4844, KAPA Biosystems, Roche), and then sequenced in a paired-ended manner using the NextSeq 500 (Illumina) according to standard Illumina protocols.