GSM7928272: CM kucg-2 CRIPSRi sgRNA diff screen rep2; Homo sapiens; OTHER
5*106 cells were harvested per screen and gDNA was extracted using the Nucleospin Blood Kit (Macherey&Nagel). Per screening condition, 20 μg of DNA was amplified using NEBNext Ultra II Q5 Mix with two primer sets flanking the sgRNA sequence sgRNA-seq with costum sequencing primer