GSM7124074: Ipilimumab; Mus musculus; RNA-Seq
Tumors from CTLA-4-humanized MC38 syngeneic mice (n = 3 - 5 mice) were extracted for single cell suspension using a gentleMACSTM Octo Dissociator. The cells were stained with anti-mouse CD45 antibody (PE-Cy7, Clone 30-F11, Cat. 103114, Lot: B354212) and sorted with a BD FACSAriaII Cell Sorting Flow Cytometer Library preparation of CD45+ sorted cells for single-cell- RNA sequencing (scRNA-seq) was performed using the 10X Genomics Chromium Single Cell 5' Library & Gel Bead reagent kit. For TCR sequencing, the Chromium Single Cell V(D)J Enrichment kit was used. scRNA-seq and TCR-seq