GSM7898098: Visium_heart_7dPI; Mus musculus; OTHER
Mice were anesthetized with 0.5 mg/g tribromoethanol (Avertin) IP.  Following transcardiac perfusion with 20 mL of PBS-EDTA (2mM), whole hearts were excised and their atria were trimmed off. For spatial RNAseq, heart samples were washed with cold PBS upon harvest and directly snap frozen in OCT compound. Using a Leica cryostat, a 10-um transverse section of the heart was mounted onto a glass slide. The section was then fixed in cold methanol prior to H&E staining (7 minutes hematoxylin and 1 minute eosin incubations) and imaged with a Nikon Eclipse Ni microscope. Further processing of tissue sections was performed in accordance with the Visium CytAssist user guide (CG000495) using the Visium CytAssist Spatial Gene Expression Kit (PN-100051). For spatial RNAseq, cDNA libraries were generated using the 10x Genomics Visium Spatial Gene Expression 3' Library Construction V2 Kit, followed by sequencing on an Illumina NextSeq 2000 using the P2 100 Cycle kit to a depth of 40,000 mean reads per spot.