GSM8224237: H9,SIRT1 KO, day 6 of differentiation, scRNAseq; Homo sapiens; RNA-Seq
hESCs from the H9 cell line were detached using TrypLE Expres directly in the culture plate, the plates were then incubated for 5 minutes at 37°C. Subsequently, 1 ml of mTeSR1 medium was added to each well to recover the cells into a 15 ml Falcon tube. The cells were centrifuged at 1000 rpm for 4 minutes, and the supernatant was removed. The cell pellet was gently resuspended in 1 ml of 1x PBS, followed by a second centrifugation under the same parameters. After removing the supernatant, the cells were resuspended in 500 μl of mTeSR1 medium and filtered using a 5 ml Falcon tube with a 35 μm cell strainer. The library was performed using the 10x Chromium Next GEM Single Cell 3' Reagents kit protocol (Document number. CG000204).