GSM8004263: PA14 trmA mutant rep2; Pseudomonas aeruginosa; RNA-Seq
The cell pellets were lysed in Trizol reagent. Total RNA was isolated using Purelink RNA mini kit (Ambion). The extracted total RNA was treated with DNaseI followed by analysis using Agilent 2100 Bioanalyzer (Agilent Technologies). Depletion of rRNA was performed using NEBNext rRNA depletion kit. The library was prepared using NEBNext Ultra II Directional RNA library preb kit.