GSM6689459: RPE-1_DMSO_3; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's protocol. Libraries were prepared from 200 ng of input material (total RNA) using NEBNext Ultra II Directional RNA Library Preparation Kit in combination with NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA) following the manufacturer's instructions (New England Biolabs). Quantification and quality checked of libraries was done using a 4200 Tapestation instrument and a DNA 1000 kit (Agilent Technologies). Libraries were pooled and sequenced on a NovaSeq 6000 SP 100 cycle v1.5 run. System runs in 101 cycle/single-end/standard loading workflow mode.