Shotgun metagenomics of soils from a TX rainfall gradient after release from long-term drought
Total genomic DNA was extracted from two 0.25g aliquots using DNeasy Power Soil Kits (Qiagen, Germantown, MD, USA) and combined, then quantified fluorometrically, and normalized to 0.5 ng/L. Libraries were prepared following Baym et al. (2015 PLoS ONE  https://doi.org/10.1371/journal.pone.0128036) using Nextera adapters and sequenced (2  150 bp) with Illumina NovaSeq 6000 at the NC State University Genome Sequencing Lab., Adapters: N706, S503