GSM7978790: IARS1 mutation 1; Homo sapiens; RNA-Seq
the PBMCs were isolated through density gradient centrifugation using Ficoll-Paque Plus medium (GE Healthcare) and processed within 1 hour. With survival rate of over 85%, the single cell suspensions were washed and resuspended at a concetration of 700-1200 cells/ul using the Chromium Next GEM Single Cell V(D)J Reagent Kits(10x Genomics) according to the manufacturer's instructions. Based on a target cell count of 10,000-13,000, GEMs (Gel Bead in Embusion) were created for single cell isolation. These GEMs were collected by oil breaking treatment and subjected to reverse transcription PCR for labeling. Subsequently, cDNA, along with cell barcodes, underwent PCR amplification. The single-cell RNA sequencing (scRNA-seq) libraries were prepared using the 5' Library Kits (PN-1000165, PN-1000020)