GSM8069085: BMDM WT UN rep 3; Mus musculus; RNA-Seq
RNA was harvested using Trizol reagent. Zymo Research Direct-zol kits were used to isolate total RNA. 1 ug of total RNA per sample was sent for library construction and next generation sequencing. Ribodepletion library preparation was carried out by the Baylor  College of Medicine Genomic and RNA Profiling Core (GARP) in biological triplicate. RNA sequencing (150 bp paired-end reads) was performed on an Illumina Novaseq 6000 with S4 flow cell Total RNA-Seq with ribodepletion