H3K27me3 ChiP-seq of homo sapiens: old female blood replicate 1
Cells were thawed on ice and sonication was performed using Low Cell ChIP kit (Cat. no. Diagenode C01010072) to get DNA fragmentation in size of 200-400bp. Diagenode antibodies H3K27me3 (Cat. No. C15410195) was used for chromatin immunoprecipitation using Low Cell ChIP kit (Diagenode C01010072) following manufacturer's instructions. Immunoprecipitated DNA was purified using IPure Kit (Diagenode C03010015), and it was subsequently used for NGS library preparation using Applied Biosystem SOLID5500 library preparation kit (Part no. 4464418) following manufacturers instructions.