GSM7842689: hlh_30_Her_1; Caenorhabditis elegans; RNA-Seq
Total RNAs were extracted from prefertile adult to day 1 adult WT and hlh-30(tm1978) hermaphrodites and males, three times independently. Synchronized hermaphrodites were harvested using S-basal buffer. Males were picked and harvested using S-basal buffer every day. One set of total RNA of males was extracted from the samples collected for about 20 days. Total RNA was isolated using RNAiso plus following the manufacturer's instructions. cDNA library was prepared for paired-end RNA seq using Illumina NovaSeq 6000 platform.