GSM7050528: B1-8 BTKWT V7; Mus musculus; RNA-Seq
Single LZ B cells were sorted into 5 uL of TCL Buffer (Qiagen). RNA was purified using magnetic beads (RNAclean XP, Beckman Coulter) Template-switched cDNA was generated and pre-amplified. Tagmentation was performed with an Illumina DNA Prep kit and indexed using IDT for Illumina Index Sets. SMART-Seq2