GSM8223511: NpatKI, dTAG, 0hr, rep1; Mus musculus; ATAC-seq
5 x 104 mouse AML cells were lysed in ice-cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Transposition was then carried out using Tn5 Transposase (Tagment DNA TDE1 Enzyme, Illumina). Transposed DNA fragments were purified using a MinElute Kit (Qiagen), followed by PCR amplification with specific PCR primers. Amplified libraries were further purified with the MinElute Kit.