1-Bd21-3-3h
Bd21-3 submergence for 3h. RNA libraries were constructed by using Truseq Stranded mRNA library preparation kit (Illumina). All procedures followed the protocol provided by the manufacturer. The steps of library constructions included mRNA isolation, RNA fragmentation, cDNA synthesis, adapter ligation and DNA enrichment, starting with 4 g of total RNA. All sequencing data were generated using Illumina sequencing, following standard protocols, with the nextSeq 2000 platform.