GSM7123557: PER-403_taz+ABBV-07_IgG_rep2; Homo sapiens; OTHER
Human cells were mixed with Drosophila cells at a 4:1 ratio and then cells were bound to magnetic beads and permeabilized with digitonin. Permeabilized cells were incubated with the indicated antibody plus an anti-Drosophila Histone H2Av antibody (Active Motif Spike-in Antibody, #61686) overnight. Antibody-bound chromatin was released by in situ protein A-MNase digestion and the soluble DNA then purified. DNA was prepared for next-generation sequencing using a NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs).