GSM6509110: ILCs_CCRL2_KO; Mus musculus; RNA-Seq
Mice were sacrificed and lungs and tumors harvested. The innate lymphoid cell populations (NK, ILC1 and ILC2) were sorted from lung tumors by using a Becton Dickinson FACS Aria Fusion (BD Biosciences) and Single cell sequencing libraries were generated using BD Rhapsody™ Single-Cell Analysis system (BD) according to the manufacture's protocol. Both cell viability and counts were then evaluated by the BD Rhapsody scanner, and only samples with high viability (> 85 %) were used for sequencing. Each genotype was loaded in a dedicated BD Rhapsody cartridge. RNA was extracted according to the manufacter's instructions (BD Rhapsody™ Single-Cell Analysis System ). Library was performed according to the manufacter's instructions (BD Rhapsody™ Single-Cell Analysis System ).