GSM7184540: scRNAseq from day 20; Homo sapiens; RNA-Seq
FLOs on day 10 and hFLOs on days 13, 16 and 20 were singularized with Trypsin, cryopreserved in CellBanker and sent overnight to GENEWIZ/Azenta Life Sciences (New Jersey, USA). In brief, at GENEWIZ the manufacturer's instructions for scRNA-sequencing were subsequently followed utilizing 10x Genomics Chromium; cells and barcoded gel beads were separated in partitioning oil droplets. Cell- and transcript-specific barcodes were added during reverse transcription. The Illumina platform was used to pool and sequence barcoded libraries.