GSM8224282: PAW3; Escherichia coli; RNA-Seq
Lysozyme and proteinase K were utilised to enzymatically lyse and digest the E. coli biofilm cells, and RNA extracted as per RNeasy® Mini Kit manufacturer's protocol (Qiagen). The library preparation and ribosome depletion were carried out using the Illumina Stranded Total RNA prep Ligation with Ribo-Zero Plus kit according to the manufacturer's protocol (Illumina, San Diego, CA, USA).