GSM6593355: LECs, E16; Mus musculus; RNA-Seq
The  lymphatic endothelial cells (CD45–CD31+GFP+) were sorted from mouse lungs at E14.5, E16.5, E18.5, and P0 (3 to 5 mice were pooled for each experiment group). The lymphatic endothelial cell suspensions were co‐encapsulated in droplets with barcoded beads by using the Chromium Controller (10X Genomics). Single cell cDNA synthesis, amplification and sequencing libraries were generated using the Single Cell 3' Reagent Kit as the 10x Genomics protocol. The libraries were sequenced by Illumina HiSeq 2500.