GSM7090962: BM-081018-N1; Mus musculus; RNA-Seq
Intestinal leukocytes were isolated by dissecting 4 cm intestinal segments and removal of Peyer's patches. The segments were opened longitudinally and rinsed in PBS to remove the remaining feces. Epithelial cells were separated from the lamina propria using cell dissociation (CD) buffer (HBSS without Mg2+, Ca2+, 5 mM EDTA, 10 mM HEPES). The remaining tissue was digested with digestion buffer (HBSS with Mg2+, Ca2+, collagenase D 0.5 mg/ml, DNAse 0.5 mg/ml) to obtain single-cell suspensions. As described in CEL-Seq2 protocol (Hashimshony et al. 2016) and its modified version (Herman et al., 2018). Adapted from TruSeq Small RNA Library Preparation Protocol