GSM7979074: Brd4 short isoform-V5 tagged; Mus musculus; ChIP-Seq
Chromatin was prepared using ChIP-IT Express Enzymatic kit (Active Motif). Cells were fixed with 1% formaldehyde for appropriate time (5 minutes for H3K27ac, H3K27me3, H4K20ac and p300; 10 minutes for Nat10, Brd4-pan, Brd4-SF-V5 tag, Rrp1b endogenous, Rrp1b-HA tag and Sipa1). After lysis, chromatin was sheared using enzymatic digestion cocktail for 10 minutes. Library was generated using SWIFT Accel-NGS 2s Plus DNA library prep kit according to manufacturer's instruction