Illumina NovaSeq 6000 paired end sequencing; RNA-sequencing of forward programmed hepatocytes and fetal liver samples
RNA-sequencing of forward programmed hepatocytes and fetal liver samples
Human fetal liver tissue was dissected from third trimester embryos, from patients undergoing elective termination.  RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the NGS library facility of the Wellcome â€“ MRC Cambridge Stem Cell Institute (Cambridge, UK).