GSM7090426: ST4KO2; Mus musculus; RNA-Seq
Following culture, live CD4 T cells were isolated by centrifugation over Histopaque 1083 (Sigma) followed by Dynabeads FlowComp Mouse CD4 kit (Invitrogen). RNA was isolated from WT and STAT4-/- CD4 cells using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's protocols. After RNA purification, cDNA synthesis and a standard RNA-seq library preparation was performed by GENEWIZ.