GSM8181628: motor neuron, control SFC-180; Homo sapiens; RNA-Seq
iPSC-MNs for RNA sequencing were harvested on day in vitro 53, iPSC-SNs were harvested on day in vitro 45. RNA extraction was performed using the QIAGEN RNeasy Micro kit. RNA abundance and quality were assessed using nanodrop and the Bioanalyzer Nano RNA kit. Library preparation was done using Illumina Truseq and paired-end sequencing with a read length of 150bp was performed on the Illumina NovaSeq 6000.