GSM8222865: H_40pct_bio_rep_3; Homo sapiens; RNA-Seq
Retinal punches were dissolved in 800uL Trizol Reagent (Invitrogen #15596026). 200uL chloroform was added to each sample, inverted, incubated at room temperature for 3 minutes. Samples were then spun down at 12,000 3 g at 4 C for 15 min. The aqueous later was transferred to a new tube and 1uL glycogen and 500 uL isopropanol was added to each sample and mixed by vortex. Samples were incubated at room temperature for 10 minutes and spun down at 12,000 3 g at 4 C for 15 min. The isopropanol was removed and the pellet was washed twice with ice-cold 80% EtOH. The washed pellet was resuspened in water. Libraries were constructed using Illumina library prep acording to manufacturer protocols