GSM8136889: sample7 early atrial fibrillation; Homo sapiens; RNA-Seq
cell：Each sample was washed, chopped into small pieces, and digested in enzymatic digestion buffer with 450 U/ml collagenase I (Sigma, C0130), 125 U/ml collagenase XI (Sigma, C7657), 60 U/ml DNase I (Roche, 04536282001) and 60 U/ml hyaluronidase (Sigma, H3506) at 37 °C for 30 minutes while shaking.1 The digested samples were filtered through 40-μm cell strainers (BD, 352340), washed, and centrifuged at 300g for 10 minutes.　nuclei：LA samples were cut into small pieces and frozen into liquid nitrogen within 8 hours after sample collection. Nuclei isolation was performed according to Chromium Nuclei Isolation Kit with RNase Inhibitor (PN-1000494). Frozen tissue samples are homogenized with a pestle in Lysis Buffer and passed through a column. Next, debris is removed via centrifugation in Debris Removal Buffer. The isolated nuclei are then washed and resuspended and loaded directly into compatible 10x Genomics protocol for making libraries.