GSM7763799: OPM2-scRNAseq-2hours_All_samples_multiplexed; Homo sapiens; RNA-Seq
Cells were harvested, pelleted by centrifugation, and resuspended in FACS buffer (2% FCS in PBS) then incubated with Cell Hashing antibodies (BioLegend) for 20 minutes on ice. Next, cells were thrice washed in FACS buffer followed by resuspension in PBS supplemented with 0.04% BSA at a concentration of 1500 cells/µL, and then each of the treatment conditions were pooled together into a single tube. Cells were loaded onto the 10x Chromium instrument (10x Genomics) to generate single-cell gel beads in emulsion and barcode cells. For 5′ multiomics 5′ hashtag oligos (HTO) was prepared using the Chromium Single Cell 5′ Feature Barcode Library Kit and indexed using the Chromium i7 Multiplex Kit N, Set A.