GSM7660669: healthy control, line 1, scRNAseq; Homo sapiens; RNA-Seq
Day 85 cultures from 16 lines were detached enzymatically in parallel using papain (Worthington; LK003153) and were filtered through 40µm Flowmi Cell Strainers (Scienceware; H13680-0040) to obtain a single cell suspension. The ran on a 10x Chromium Controller followed by RNA extraction in GEMs according to manufacturer's instructions (10x Genomics Single Cell 3' v3) Library was prepared according to manufacturer's instructions (10x Genomics Single Cell 3' v3)