GSM7184728: HCT16_23D; Homo sapiens; RNA-Seq
Human eosinophil RNA was isolated using TRIzol (Ambion) supplemented with RNasin ribonuclease inhibitor (Promega). RNA was extracted by the addition of chloroform followed by centrifugation and addition of absolute ethanol to the top layer. Thereafter, the fluid was transferred to PureLink RNA mini kit (Invitrogen) and processed according to manufacturer's instructions. TheRNAintegrity number (RIN) was analyzed using Bioanalyzer and only samples of RIN>6.3 were used. RNA libraries were generated using Cell-seq Library protocol, and sequenced on Illumina HiSeq2500, 50 single-end run. Sample preparation, sequencing, quality control, and normalization were conducted by the "Technion Genome Center," Life Science and Engineering Interdisciplinary Research Center, Technion, Haifa, Israel. RNA libraries were prepared for sequencing using Cell-seq protocols