GSM6629094: Bulk BCR, Subject MuSK MG-1 at 70 months; Homo sapiens; RNA-Seq
RNA was isolated from frozen PBMCs using the RNeasy Mini Kit (Qiagen, 74104) according to manufacturer's instructions. Bulk BCR libraries were either generated using the NEBNext Immune Sequencing Kit (NEB) or the SMARTer® Human BCR IgG IgM H/K/L Profiling Kit (Takara Bio USA, Inc.) using the primers targeting IgA, IgG and IgG-sub.   Single-cell libraries were generated from sorted B cells enriched from cryopreserved PBMCs, using the Chromium Single-cell 5′ Reagent Kit (10x Genomics; V 2.0) according to manufacturer's instructions.