GSM8084708: KDM5A, cDC1, CUTTag, BD_A2; Mus musculus; OTHER
The fragment DNA were extracted by 150 µl tris saturated phenol and 150 µl chloroform. CUT&Tag for the B16-OVA cells and cDC1s were carried out according to the instruction of CUT&Tag assay kit (Vazyme).  The PCR on the CUT&Tag was performed with the following protocol: 3 min at 72 °C and 3 min at 95 °C, followed by 15 cycles of 10 s at 98 °C, and 5 s at 60 °C, with a final extension at 72 °C for 1 min.