GSM8224932: EL_1hr_TL_rep2; Homo sapiens; OTHER
RNAs were extracted using TRIzol according to manufacturer's instructions.10 µg of RNA were treated using 0.4 units of TURBO DNase (ThermoFisher) per microliter in 200 µL reactions  incubating samples at 37ºC for 30 minutes.  RNAs treated with TURBO DNase were recovered using RNA XP beads (Beckman). RNAs were then subjected to TimeLapse chemistry (Schofield et al. 2018) by incubating samples at 45ºC for 1 hour with 600 mM TFEA, 1 mM EDTA, 100 mM sodium acetate pH 5.2 and 10 mM sodium periodate and then reduced by incubating samples at 37ºC for 30 minutes with 100 mM DTT, 100 mM Tris pH 7.4, 10 mM EDTA and 1mM NaCl. For 1hr treatments, libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina). For 2hr treatments, the SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian kit was used to prepare libraries. TL-seq