GSM7050728: indir_D22; Homo sapiens; RNA-Seq
Single cell suspensions were created differently based on the stage of the differentiation. D6 neural crest induction cultures were dissociated with Accutase for 15min at 37°C with 5% CO2. D15, D22 and D30 crestospheres were dissociated with Accutase for 15, 20 and 30min, respectively, at 37°C with 5% CO2. D40 cultures were dissociated with 0.05% Trypsin for 1min at 37°C, with 5% CO2. T1 and T2 melanocyte cultures were dissociated with Accutase for 5-10min at 37°C with 5% CO2. snRNA-seq libraries were prepared with Chromium Next GEM Single Cell 3′ Kit v2 (10x Genomics). The libraries were sequenced at SF Chan Zuckerburg Biohub.