GSM6820560: 4006_LA_HighDoseSaCas9Nuclease; Mus musculus; RNA-Seq
Total RNA (1 ug) underwent two rounds of mRNA purification (polyA-selection) using Dynabeads mRNA DIRECT Kit (Invitrogen, Carlsbad, CA). Double-stranded cDNA was generated using the Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). We performed PCR amplification of the targeted MYH6 R403Q allele. PCR products were fragmented using an NEB Fragmentation Kit and Illumina sequencing libraries were prepared. The cDNA products were used to construct libraries with single end 190 bp read and a single I plex 9 bp read.