GSM8224105: mtDNA_2_RNAseq [A5]; Mus musculus; RNA-Seq
Total RNA was isolated from FACS-sorted pDC treated as indicated. Cells were homogenized with TRIzol reagent and purified using RNeasy Mini Kit (Qiagen). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP.