GSM7090957: Cre_pos_rep1; Mus musculus; RNA-Seq
Cortical tissue was submerged in Trizol reagent (Invitrogen/ThermoFisher) and mechanically dissociated using a bead homogenizer. Chloroform extraction was performed, and the supernatant was collected and mRNA was isolated using Ambion PureLink RNA iolation kit (ThermoFisher), following the manufacturers instructions. Library preparation was performed with 10ng of total RNA for samples with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol.