GSM8181922: pair41cont1-P-BL [PC_126]; Homo sapiens; RNA-Seq
RNA was extracted from frozen TZ biopsies at baseline and follow-up. Prior to extraction, excess OCT from each biopsy sample was physically removed on an aluminum block surrounded by dry ice to ensure the tissue does not thaw. Frozen tissue was sectioned to 20 µM slices QIAzol ensuring that the sample is submerged in the liquid and stored at -80C. RNA extraction used the miRNAeasy kit from QIAgen (Item # 69989) following manufacturer instructions RNA samples were sequenced at Novagene in a blinded manner by Illumina HiSeq 2500