GSM7392757: PBMC, healthy, rep4-6; Homo sapiens; RNA-Seq
KIR3DL3+ lymphocytes were enriched with PE-magnetic beads from cryopreserved PBMC. Cell subsets for the populations from different donors were labelled simultaneously with the cell surface sorting panel an oligonucleotide-tagged antibody sample tag (BD Biosciences) prior to cell sorting on a FACSAria3 (BD Biosciences). Library was performed according to the manufacter's instructions (TCR/BCR Full Length, mRNA Whole Transcriptome Analysis (WTA),  BD® AbSeq, and Sample Tag Library Preparation Protocol, BD Biosciences). Prior to cDNA library preparation for the WTA and VDJ libraries, all cell subsets from the different donors were pooled, up to 12 unique sample tags were combined per library. Briefly, cells were captured with beads in a microwell plate, followed by cell lysis, bead retrieval, cDNA synthesis, template switching, Klenow extension, and library preparation.