Genome-wide CRISPR activation screen in SK-N-DZ neuroblastoma cells: guide RNA distribution following Erastin treatment (1 M), replicate 3
Genome-wide CRISPR activation screen in SK-N-DZ neuroblastoma cells. Guide RNA distribution of the Calabrese library in Erastin-treated cells (1 M); replicate 3. SK-N-DZ cells stably expressing dCas9-VP64 were transduced with the Calabrese library (Set A) at a multiplicity of infection of 0.3. After puromycin selection and recovery, cells were collected, genomic DNA extracted and amplicon libraries constructed for Illumina sequencing.