GSM7764505: Ube2iF/F siEtv1_2 Replicate 1; Mus musculus; RNA-Seq
RNA (100-200 ng/ul) was isolated using the RNeasy Plus Mini Kit, knockdown confirmed with RT-PCR and protein Western blot. RNA (~20 ug) was submitted to the sequencing core at University of Nebraska Medical Center for library construction and RNA sequencing with 75 bp paired-end reads on Novaseq6000 platform.