GSM8003966: Newborn lens fiber cell miRNAs_2; Mus musculus; RNA-Seq
For miRNA-sequencing: Total RNA was extracted using mirVanaTM miRNA isolation kit (# AM1560, ThermoFisher Scientific). Small RNA was isolated from total RNA by size-selection and NEBNext Multiplex Small RNA Library Prep Kit was used for 50bp-single-ended sequencing. For mRNA sequencing Total RNA was extracted using the RNEasy Mini Kit (Qiagen cat. 74104) following the manufacturer's instructions. For miRNA libraries were prepared in Genomics and Sequencing core, University of Cincinnati using NEBNext Multiplex Small RNA Library Prep Kit. For mRNA sequencing RNA libraries were constructed by Novogene (Sacramento, CA, USA) using a TruSeq (Illumina) library prep kit.