GSM6628141: DIPG010_TEPA_rep2; Homo sapiens; RNA-Seq
Cells were washed with PBS, and then lysed using the Qiagen RNA Extraction Kit. The RNA purity and concentration was then assessed using the Nanodrop. Quality control and library preparation were carried out by Ramaciotti Centre for Genomics (UNSW, Australia). Paired-end 100 bp sequencing was performed on the Illumina NovaSeq 6000 using TruSeq stranded mRNA for a total of 1.6B reads.