GSM8219864: PD_M_69_Left_52_atac; Homo sapiens; ATAC-seq
Neuronal nuclei were isolated from the human prefrontal cortex using flow cytometry and filtering for an anti-NeuN antibody. DNA was isolated from the neurons using Zymo DNA concentrator Kit. Library generated using the Nextera XT DNA Library Kit with Tagment Enzyme (TDE1) on 30k nuclei, size selection to remove fragments >1kb average size 400-500bp bulk ATAC-seq on neuronal nuclei.