GSM6447733: 6182KO-Day5; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen).The concentration and quality of total RNA samples was first assessed using Agilent 2100 Bioanalyzer. A RIN (RNA Integrity Number) of five or higher was required to pass the quality control. Four hundred nanograms of RNA per sample were used to prepared dual-indexed strand-specific cDNA library using TruSeq RNA Access Library Prep Kit (Illumina).