Interferon Alpha (IFNα) Induction and Knockout Analysis of ISGs in Atlantic Salmon SHK-1 Cells Challenged with ISAV and IPNV
Interferon Alpha (IFNα) Induction and Knockout Analysis of ISGs in Atlantic Salmon SHK-1 Cells Challenged with ISAV and IPNV
RNA seq analysis was conducted on IFNα_ISAV/ IFNα_IPNV (n=4) treatment groups at 2 hours post-infection and results were compared with wild SHK-1 cells (n=4). For Knock out (KO) experiment, IFNα_ISAV/ IFNα_IPNV (n=4) treatment groups at 2 hours post-infection were used as control and below KO groups were used as treatment groups. The number of each group was mentioned in brackets. KO_CRFB1a_IFNα_ISAV_SHK-1  (n=3) KO_CRFB5a_IFNα_ISAV_SHK_1  (n=3) KO_I10R2_IFNα_ISAV_SHK-1 (n=2) KO_I10Rb_IFNα_ISAV_SHK-1  (n=3) KO_IFNGR2b_IFNα_ISAV_SHK-1  (n=3) KO_CRFB1a_IFNα_IPNV_SHK-1  (n=3) KO_CRFB5a_IFNα_IPNV_SHK_1  (n=3) KO_I10R2_IFNα_IPNV_SHK-1  (n=2) KO_IFNGR2b_IFNα_IPNV_SHK-1  (n=3) KO_I10Rb_IFNα_IPNV_SHK-1  (n=3) KO_All_IFNα_ISAV_SHK-1  (n=2) KO_All_IFNα_IPNV_SHK-1  (n=2)    Total RNA was extracted from collected cells by using the Direct-zol RNA Microprep Kits (Zymo) using the manufacturer's protocol. The RNA purity was measured using a Tape station, and the RNA concentration was determined using spectrophotometry (Nanodrop ND1000).  Novogene mRNA-seq Services did library preparation. Briefly, the library is prepared according to Salmo salar, and subsequently tested for its quality (Library QC).