GSM7897874: CRL/V, replicate 2; Mus musculus; RNA-Seq
C-kit+ and Mac1+ BM cells from WT/V, WT/N, CRL/V, CRL/N, CRS/V or CRS/N mice were flow-sorted and mixed in a 1:1 ratio. Cells were loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual gel-bead-in-emulsions (GEMs). Single-cell RNA-seq libraries were constructed using Single Cell 3' Library and Gel Bead Kit V3.1.