GSM8390825: Control; Homo sapiens; OTHER
A modified version of the iCLIP library preparation protocol outlined in Damianov et al., 2016 was followed. Briefly, L3 linker was ligated to the RNAs and samples were reverse transcriped with primers containing unique 4 nucleotide molecular identifiers for downstream PCR de-duplication. The cDNA was then size selected for molecules containing inserts between 20-80 nucleotides. cDNA was then circuliarzed with circligase and linearized with BamHI. Libraries were then prepared with PCR amplification using P5solexa and P3solexa primers. The products were run on an agarose gel and size selected.