GSM7979373: Participant 3834-007 Pre exercise; Homo sapiens; RNA-Seq
Patient muscle biopsies were homogenized in TRIzol reagent (Invitrogen) and transferred to 5 Prime - Phase Lock Gel - heavy 2ml tubes (Andwin Scientific) to purify total RNA. Total RNA (500ng) was used to initiate the Stranded mRNA-Seq library preparation using the TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina RS-122-2101/2102).