GSM8224348: MUT_D11_p2_NFIAn_rep1; Mus musculus; ATAC-seq
CaTS-ATAC was performed as previously described (Delás et al., 2023) with in-house produced Tn5. A dead cell removal step was introduced to enrich for live cells prior to fixation and transposition. The details are as follows: at the differentiation timepoint of collection, cells were washed once with PBS and dissociated with Accutase (Gibco, A1110501) for 5 min at 37°C. Cells were collected in 1.5 ml LoBind Eppendorf tubes (Eppendorf cat. #Z666548) and spun down at 400xg for 4 min. Cell suspensions were supplemented with LIVE/DEADTM Fixable Dead Cell Stain Near-IR fluorescent reactive dye (Thermo Fisher, L34976), according to the manufacturer's instructions (1 µl dye/1 ml PBS for 1x106 cells) and incubated for 30 min on ice protected from light. During the incubation, the autoMACS® Pro Separator (Miltenyi Biotec) was set-up according to the manufacturer'