GSM8022597: IP-treat; Homo sapiens; ChIP-Seq
Cultured HK-2 cells underwent indicated treatment were fixed with 1% paraformaldehyde. ChIP assays were performed with Pierce Magnetic ChIP kit (Thermo scientific, Cat. 26157) according to manufacturer's protocol.  After the lysis and MNase digestion, chromatin was sonicated on ice with three 20-second pulses at 3 watts power (using Sonicator 3000 Instrument). Immunoprecipitation was performed overnight at 4 °C.