HIFI
For PacBio HIFI sequencing, genomic DNA was extracted from fresh leaves using QIAamp DNA Mini Kit/DNeasy Plant Mini Kit1 (QIAGEN). Each SMRT bell library was constructed using the Pacific Biosciences SMRT bell template prep kit 1.0. The constructed library was size-selected by Sage ELF for molecules 11~15 Kb/14-17kb2, followed by primer annealing and the binding of SMRT bell templates to polymerases with the DNA Polymerase Binding Kit. Sequencing was carried out on the Pacific Bioscience Sequel II platform for 30 h.