GSM7290703: HHV57DSXY_19005478_S46,TT16_A,PDH_MEMORY; Mus musculus; RNA-Seq
Poly-A selected RNA-seq libraries were constructed from 1 mg total RNA using the Illumina TruSeq RNA Sample Prep Kits, version 2.  The resulting cDNA was fragmented using a Covaris E210. Library amplification was performed using 11 cycles to minimize the risk of over amplification.  Unique barcode adapters were applied to each library.  Libraries were quantitated by qPCR using the KAPA Library Quantification Kit (KAPA Biosystems) and pooled in an equimolar ratio.  The pooled libraries were sequenced on a NovaSeq 6000 with version 1 chemistry.  At least 90 million 150-base read pairs were generated for each individual library.  Data was processed using RTA 3.4.4.