GSM7050662: C57BL/6 B cells, p53-KO, pRetroX_M2.Stop_IRES_ZsGreen, Day 4 #1; Mus musculus; RNA-Seq
RNA was harvested using trizol. Library was constructed by BGI Laboratories using DNBSEQ Eukaryotic transcriptome protocol. Following mRNA isolation, the mRNA was fragmented and first strand synthesis was performed. Double stranded cDNA fragments were subjected to end-repair, and a single 'A' nucleotide was added to the 3' end of the blunt fragment. Following library QC, the single strand cDNAs were circularized and eplicated via rolling cycle amplification. DNA nanoballs were generated and loaded into patterned nanoarrays using high-intesity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS).