Illumina Genome Sequencing of O. tipulae JU177: Mixed Worms
Cultures of O. tipulae were grown on 150 mm plates of rich agar seeded with E. coli NA22. When the bacterial food source was near exhaustion, worms were washed off plates with M9 buffer and centrifuged at 170 x g for 1 min. The pelleted worms were then purified from microbial contaminants using a 35% sucrose floatation. Genomic DNA was extracted from harvested worms using Genomic-tips (Qiagen Cat# 10223). The resulting high molecular weight genomic DNA was used to prepare sequencing libraries using the Illumina DNA Prep kit (Cat# 20018704) following the manufacturers instructions.