GSM7842804: 23039Pol_32; Mus musculus; RNA-Seq
Fresh liver tissue (~20mg) was stored in DNA/RNA ShieldTM (Zymo Research) at 4 C until processed. RNA was extracted with a Quick-RNATM Miniprep Plus Kit (Zymo Research) using Proteinase K digestion and subsequent steps according to the manufacturerâ€™s protocol. mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit, miniaturized to 2/5 reaction volume and automated on a Sciclone NGSx liquid handler