GSM6448409: ECN_4+#2; Mus musculus; RNA-Seq
For flow-cytometrically sorted samples, cells  were isolated by first enzymatically digesting distal colon tissue and then purified by fluorescence-activated cell sorting (two times to achieve enhanced purity). A total of  1,000  CD4+ T cells (DAPI- CD19- CD4+ TCRβ+) were sorted using the BD Aria IIinto TCL buffer (QIAGEN) with 1% 2-mercaptoethanol and submitted for library construction and sequencing.