GSM7123843: HT1080 KDAC6H611A 3; Homo sapiens; RNA-Seq
3x10^6 cells were pelleted, resuspended in MEM with 5% glycerol, and frozen at -80 °C. Cells were shipped on dry ice, and total RNA extracted by Creative Genomics using a commercially available kit. Quality of RNA was verified using RNA Screen Tape (Agilent). RNA was rRNA-depleted using the Ribo-Zero plus kit (Illumina). Libraries were prepared using the PE150 platform (Illumina) following the manufacturer's instructions.