GSM6509508: MCF-7 cells_+DOX-TAM_Day20_sgRNAlibrary_1; Homo sapiens; OTHER
sgRNA DNA sequencing: The extraction protocol to prepare genomic sgRNA DNA was performed following the “Illumina Sequencing Sample Preparation for use with CRISPRi/a-v2 Libraries” protocol (https://media.addgene.org/cms/filer_public/d3/bb/d3bb61d4-a453-4c65-903a-a7672ab71ae2/illuminasequencingsampleprep.pdf), with minor modifications described in our manuscript. PRO-Seq: Nuclei were extracted and material was prepared following https://doi.org/10.1126/SCIENCE.1229386 and https://doi.org/10.1038/nprot.2016.086 protocols. sgRNA DNA library construction was prepared followed in “Illumina Sequencing Sample Preparation for use with CRISPRi/a-v2 Libraries” protocol ((https://media.addgene.org/cms/filer_public/d3/bb/d3bb61d4-a453-4c65-903a-a7672ab71ae2/illuminasequencingsampleprep.pdf) with the minor modifications described in our manuscript. PRO-seq RNA libraries were prepared for sequencing using standard Illumina protocols sgRNA DNA sequencing and PRO-seq