GSM6448190: RGC077; Mus musculus; RNA-Seq
The retina is isolated and flat-mounted under dim red light (created with BioRender.com). A retinal ganglion cell is patched and its responses to blue and green spotlight stimuli are recorded. The cell content is aspirated. RNA is processed using Smart-seq2 protocol to get transcriptomic data. the cDNA library was sequenced on the NovaSeq 6000 instrument and 150-bp paired-end reads were set up. Each sample was sequenced to approximately 10 million reads