GSM8390001: fs_mo_6; Homo sapiens; RNA-Seq
Nuclei were isolated from placental samples following a modified version of the Allen Brain institute protocol for nuclei extraction. Briefly, tissue was homogenized using an electric pulsator and via dounces in a glass tissue homogenizer. A series of two washes was used to remove debris, and then nuclei were stained with DAPI. Flow cytometry was used to count 10,000 nuclei per sample, with gating based on DAPI-positive cells. The standard Chromium 10X v3.1 kit instructions were followed to generate the single-nucleus RNA-seq data.