GSM6448793: ONB_scSeq; Homo sapiens; RNA-Seq
Primary biopsy specimen of treatment-naieve ONB was obtained via endoscopic surgical resection and placed in DMEM on ice. Tissue was dissociated with Dispase, followed by Accutase, followed by a brief digestion in trypsin-EDTA. The single cell suspension was cryopreserved at -80 degrees C in DMEM with 10% DMSO. Constructed using 10X Genomics Single Cell Gene Expression kit following manufacturer's instructions.