Bacteria_Grape Must_VIN_A
Several forward bacterial primers from the Ion 16S Metagenomics kit (Life Technologies, United States) were used to amplify the 16S rRNA genes targeting the V2 to V9 variable regions. A total reaction mixture of 30 L 2 Ion 16S Metagenomics Environmental Master Mix (Life Technologies, United States), 30 ng DNA template, 0.25 M of each primer, and sterile dH2O was used for PCR amplification.For bacterial communities, the amplification started at 95C for 10 min, followed by 25 cycles of 95C for 30 s, 58C for 30 s, and 72C for 20 s each, and 72C for 7 min. The presence of PCR amplicons was verified on a 1% EtBr-stained agarose gel containing 1 TAE buffer.