GSM8004267: Pseudomonas aeruginosa UCBPP-PA14 wild-type strain; bio rep 3; Pseudomonas aeruginosa; OTHER
Total RNA was extracted from exponential phase cells using Trizol reagent (Thermo Fisher scientific). Large RNAs were depleted from the total RNA solution by precipitation using 35% ethanol. Small RNA species including tRNAs in aqueous solution was then transferred into a new tube and precipitated by adding absolute ethanol at -70 °C for 2 h. tRNAs were then purified using HPLC. cDNA libraries were constructed following a revised version of the Absolute Quantification RNA Sequencing (AQRNA-seq) protocol. Other; AQRNA-seq