Iso-seq for the AEPx105 strain
RNA was isolated from AEPx105 hydra polyps utilizing the Chomczynski and Sacch method. The RNA, once redissolved in DEPC-treated water, was subjected to LiCl precipitation (2.5 M) at 4C throughout the night. The RNA, after forming a pellet, was cleansed two times using 70% ethanol, redissolved in DEPC-treated water, and conserved at -80C. The cDNA was synthesized, the SMRTbell template was prepared, and sequencing was conducted using two SMRT cells at the Genomics Research and Technology Hub at UC Irvine, employing a Pacific Bioscience Sequel II sequencer.