GSM7398588: input_rep1; Rattus norvegicus; ChIP-Seq
Cells were detached from the plate with trypsin and fixed in 1% PFA in PBS at room temperature for 8 min then quenched with 0.125 M glycine for 10 min. Crosslinked samples were then washed with cold PBS and resuspend in Farnham Lab buffer (5mM PIPEs PH8, 85 mM KCcl, 0.5% NP40) for sonication based nuclear extraction. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit complemented with NEBNext Multiplex Oligos for Illumina from NEB. NEBNext Ultra II DNA Library Preparation Kit