GSM6628998: HepG2, HBx-overexpressing, biol rep 2; Homo sapiens; RNA-Seq
Total RNA was extracted from HBx-overexpressing (HBx-oe) and NC (negative control) HepG2 cells using Trizol reagent (Invitrogen, CA) following the manufacturer's protocol. The RNA concentration and quality were evaluated with the NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies). Firstly, rRNA was depleted by NEBNext rRNA Depletion Kit（New England Biolabs, Inc., Massachusetts, USA）according to the manufacturer's protocol. Secondly, sequencing libraries were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit（New England Biolabs, Inc., Massachusetts, USA）. BioAnalyzer 2100 system（Agilent Technologies, USA）was used in quality control.