GSM7928086: follicle D6_10_5; Mus musculus; RNA-Seq
Total RNA of a single follicle was extracted using Arcturus PicoPure RNA isolation kit (Applied Biosystems, Carlsbad, CA) following manufacturer's instructions. For follicles, the cDNA library construction and low-input mRNA-Seq were performed on an Illumina NovaSeq PE150 platform by Novogene (Novogene Corporation, Sacramento, CA). For oocytes, Smart-Seq2 cDNA synthesis was performed on isolated follicle RNA with Maxima H Minus reverse transcriptase (ThermoFisher). Whole transcriptome amplified (WTA) product was purified with AMPure XP beads (Beckman Coulter), quantified with Quanit-iT PicoGreen dsDNA Assay (ThermoFisher), normalized and used for preparing paired-end sequencing libraries with Nextera XT (Illumina, #FC-131) according to the manufacturer's instructions.