16s RNA of geocarposphere soil under microplastic treatment
Microbial diversity is based on the Illumina NovaSeq sequencing platform, using the Paired-End sequencing method to build small fragment libraries for sequencing. By splicing and filtering Reads, clustering or denoising, and performing species annotation and abundance analysis, the species composition of the samples can be revealed; further alpha Diversity, beta Diversity, significant species difference analysis, correlation analysis, and functional prediction analysis can be performed to explore the differences between samples.