GSM7979080: 24h Induced BCBL-1; Homo sapiens; RNA-Seq
Total RNA was isolated from cultured cells using the TRIzol reagent (Ambion) as per manufacturer's protocol. Poly(A)-tailed RNA was selected from 75 μg of each RNA sample using Dyna Beads mRNA purification kit (Invitrogen, Catalog No. 61006). Direct-RNA sequencing libraries were prepared by using SQK-RNA002 (ONT) library preparation kit with 50 ng of each polyA-selected RNA sample. direct-RNA Sequencing