GSM6430845: Hela shHCAR1b+ RNAi δS305A HCAR1 with PBS replicate 3; Homo sapiens; RNA-Seq
Equal number of cells were seeded in 10cm dish and let to grow a density of 70-80% confluency. Lactate was added to the final concentration of 10mM (or equal volume of PBS) and incubated to 6 hours. RNA was extracted with RNeasy mini Kit (Qiagen). Samples were sent to IRIC genomic platform for analysis and sequencing. RNA integrity and quantity was validated with Bioanalyzer and then used for sequencing. Samples were analyzed with bioanalyzer for quality control and single-end NGS was performed at IRIC genomic platform with Nextseq 500 illumina system. Samples were sequenced with Nextseq 500 illumina system with a depth of ~35M per sample with single-end 75 cycles. Each sample was sequenced at least in triplicates.