GSM7050708: Rep1 CUT&Tag, wt, replicate 1; Saccharomyces cerevisiae; OTHER
For ChIP-seq, cells were fixed with 1% formaldehyde for 30 minutes, then quenched with glycine (125 mM) for 5 minutes. After cell lysis, chromatin was sonicated using an immersion tip sonicator. For CUT&Tag, cells were spheroplasted using Zymolase. Nuclei were processed using the CUT&Tag-IT Assay Kit from Active Motif using the manufacturer's instructions. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA kit (NEB, product number E7645). The CUT&Tag protocol results in ready to sequence libraries after the final PCR amplication step and cleanup.