Targeted sequencing of human B cell receptors: adult male PBMCs
B cells specific to DNA-barcoded-HIV-1 and HCV envelope glycoproteins were sorted from PBMCs of a chronically HIV-1/HCV co-infected adult. Cells were distributed into individual microfluidic droplets containing unique cell-specific oligonucleotides using the 10X Genomics Chromium Controller. cDNA libraries from single cells were further amplified with the Chromium Single Cell Human BCR Amplification Kit.