ADH1-gene target 1 Cas9 control
Arabidopsis plants were transformed via floral dip using the Agrobacterium strain GV3101. T1 plants were grown on germination medium with cefotaxime (500 mg/l) and phosphinothricin (6 mg/l) as selection marker. DNA was extracted from batches of 30 plants after 2 weeks of cultivation. Amplicons with universal adapter sequences were generated with 30 ng of genomic DNA using a proof-reading polymerase.