GSM7927837: EP2/EP4KO NK cells, untreated, unstimulated, rep4; Mus musculus; ATAC-seq
NK cells were washed in cold PBS and lysed. The transposition reaction was incubated at 37°C for 30 min. DNA was purified using the PCR & DNA Cleanup Kit (Monarch). Extracted DNA was amplified for four cycles. After evaluation by real-time PCR, 6-10 additional cycles were performed. The amplified samples were purified and size selected using SpeedBeads (Sigma) in 22% PEG.