GSM6373813: Tomato-; mesLN; mesLN after transfer; naive; Mus musculus; RNA-Seq
RNA was isolated using the Rneasy Micro kit from Qiagen; RNA was collected in 14 µl Rnase-free water and 11.5 µl were used for cDNA generation Library was prepared according to "Werner, A., et al., Determining immunoglobulin-specific B cell receptor repertoire of murine splenocytes by next-generation sequencing. STAR Protocols, 2022. 3(2): p. 101277" with addition of a primer specific for IgE