GSM7927818: PANC-1-CGN cell CGN IP1; Homo sapiens; RIP-Seq
CGN-specific antibodies captured the target protein to increase the enrichment of CGN protein-RNA complex. Subsequently, the complex's protein and RNA were analyzed and detected. RNA was isolated from the complex, and gene-specific analysis techniques (such as PCR, qRT-PCR) or high-throughput sequencing were employed to analyze both the quantity and type of RNA present. The RIP RNA was assessed by Agilent 2200 TapeStation (Agilent Technologies, USA) and Qubit (Thermo Fisher Scientific, USA). The RIP RNA was fragmented to approximately 200bp and underwent first-strand and second-strand cDNA synthesis, followed by adaptor ligation and enrichment with a low-cycle according to the instructions of NEBNext® Ultra RNA LibraryPrep Kit for Illumina (NEB, USA). The final library product was assessed with Agilent 2200 TapeStation and Qubit® (Life Technologies, USA). It was subsequently sequenced on Illumina (Illumina, USA) platform with pair-end 150bp at Ribobio Co. Ltd (Ribobio, China).