GSM7979132: inf-BL9; Mus musculus; RNA-Seq
RNA was extracted from gastric tissue obtained from  H. felis-infected and uninfected WT, Myd88-/-, TrifLps, and DKO mice. Gastric tissue samples were homogenized in 1 ml of TRIzol reagent (Invitrogen, Carlsbad, CA) following the manufacturer's instructions. RNA was extracted using the Direct-zol RNA mini kit (Zymo Research, Irvine CA) as per the manufacturer's instructions and stored at -70°C until further use. The quality of the extracted RNA was assessed using a Nanodrop system (Thermo Fisher, Waltham MA) by measuring absorbance levels at 260 and 280 nm. Samples were processed into RNA-seq libraries using the Illumina TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero (Human/Mouse/Rat), which involved ribosomal RNA depletion, fragmentation, cDNA synthesis, and cDNA library preparation.