GSM8137556: WT primary hepatocyte_Veh_AR; Mus musculus; ChIP-Seq
The cells were fixed with 0.1% formaldehyde for 2 minutes, followed by quenching the cross-linking reaction using a 10× glycine solution. Subsequently, the samples underwent cut&run assay using the CST kit (#86652) as per the provided protocol. ChIP-Seq libraries were prepared using the MGIEasy Universal DNA Library Prep Set/MGI/1000006986,  (1) DNA-end repair, 3'-dA overhang and ligation of methylated sequencing adaptor. (2) PCR amplification and size selection (usually 100-300bp, including adaptor sequence). (3) Qualified library for sequencing.