DNA-seq:HumanTumor:B5
DNA was extracted with QIAGENs QIAamp DNA Mini Kit. gDNA was sheared into fragments of the average size of 250-300 base pairs in a Covaris plate with an E220 system (Covaris, Inc. Woburn, MA) followed by end-repair (NEBNext End Repair Module  E6050L) and A-tailing (NEBNext dA-Tailing Module  E6053L), ligated with Illumina Dual index PE adapter using (Life Tech. ExpressLink Ligase- A13726101),  Pre-capture Ligation Mediated-PCR (LM-PCR) was performed for 6-8 cycles using the Library Amplification Readymix containing KAPA HiFi DNA Polymerase (Kapa Biosystems, Inc., Cat # KK2612). Universal P1.1 primers were used to amplify the ligated products.  AMPure XP beads (A63882, Beckman Coulter) were used for library purification.  Quantification and size distribution of the pre-capture LM-PCR product were determined using the Fragment Analyzer capillary electrophoresis system.