GSM8389838: Mac, DENV+IgG, scRNAseq; Homo sapiens; RNA-Seq
Single-cell RNAseq libraries were generated using the Chromium Single Cell 5' v2 Reagents Kit (10x Genomics) and the Chromium Single Cell Analyzer (10x Genomics). Indexed sequencing libraries were generated using the Chromium Single Cell 5' v2 Reagents Kit (10x Genomics) according to the manufacture's protocol (User guide RevC). according to the manufacture's protocol (User guide RevC) Library construction was performed according to the manufacturer's instructions (single cell 5' v2 protocol, 10x Genomics). In short, sequencing libraries were generated by 1) transcript fragmentation and end-repair, 2) post-fragmentation size-selection using SPRIselect beads, 3) Adaptor ligation and 4) sample index PCR and SPRIselect cleanup