GSM7842682: KIT+ cells, E12 FL, scRNAseq; Mus musculus; RNA-Seq
The fetal liver isolated from E12 mouse embryos was mechanically dissociated with a 23G needle. Those obtained cells were stained with KIT antibody and sorted using an SH800 cell sorter (SONY). Sorted KIT-expressing cells were resuspended in 0.04% BSA/PBS. Library construction was performed by using the Chromium Single Cell 3' v3.1 kit (10x Genomics).