GSM8224590: Ctrl, B1-8hi, eLPre, rep1; Mus musculus; RNA-Seq
Cells were lysed in TRIzol reagent (Invitrogen) and the RNAs were isolated with RNA Clean & Concentrator-5 (Zymo Research). rRNAs were removed using MGIEasy rRNA Depletion Kit V1.2 (MGI Tech) and cDNA library was prepared using MGIEasy RNA Directional Library Prep Set V2.1 (MGI Tech)