GSM8223900: VH-07_S38_AMKO-PBS; Mus musculus; RNA-Seq
At postnatal day 5, 6 hours after the third dose of PBS or LPS, we extracted lung tissue from the study animals, immediately snap-froze it in liquid nitrogen, and stored it at − 80°C for subsequent RNA isolation.  Total lung RNA was isolated from the lung tissues of mice exposed to PBS or LPS (n=3/genotype/exposure) and purified using the Direct-zol RNA MiniPrep kit (Zymo Research, Irvine, CA; R2052). RNA-seq libraries were next generated using the NEBNext Ultra II Directional RNA-Seq kit (p/n E7760) with rRNA depletion (p/n E7400), according to the manufacturer's instructions.