GSM6624973: Bd21 S ZT24 biol rep2; Brachypodium distachyon; RNA-Seq
Aboveground tissue was collected, frozen immediately in liquid nitrogen and stored at − 80ºC. Tissue was ground to powder with a mortar and pestle with liquid nitrogen, avoiding thawing. Total RNA was extracted with the kit Direct-zol RNA mini prep kit (Zymo Research, R2050) and digested in-column with provided DNAse I. RNA libraries for RNA-seq were prepared using TruSeq RNA sample prep v2 following manufacturer's protocols (Illumina).