GSM8022603: RKO 25A_2; Mus musculus; RNA-Seq
Total mRNA was extracted with EZ-10 Spin Columns Total RNA Miniprepes Super Kit from Bio Basic accroding to manufacter's instruction. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primer followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adpater ligation, size selection, ampification and purification.