RNAseq of Aedes aegypti larvae at L2 stage - transgenic OpIE2-loqs2
After quality control using Bioanalyzer, RNAs were polyA selected using the kit NEBNext Poly(A) mRNA Magnetic Isolation Module and libraries were subsequently constructed using the kit NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs inc.), following the manufacturer protocol.