RNA-seq of NRRL_YB-756:control pH 0h
Purified DNase treated total RNAs were run on a Fragment Analyzer (Agilent, CA) to evaluate RNA integrity. The total RNAs were converted into individually barcoded polyadenylated mRNA-seq libraries with the TruSeq Stranded mRNA Sample Prep kit (Illumina, CA). Libraries were barcoded with Unique Dual Indexes (UDIs) which have been developed to prevent index switching. The adaptor-ligated double-stranded cDNAs were amplified by PCR for 8 cycles with the Kapa HiFi polymerase (Roche, IA). The final libraries were quantitated with Qubit (ThermoFisher, MA) and the average cDNA fragment sizes were determined on a Fragment Analyzer.