GSM8085798: HDP1_DIP; Plasmodium berghei ANKA; ChIP-Seq
Recombinant homeodomain fused with GST  was incubated with P. berghei ANKA genomic DNA fragments in binding/washing buffer (10 μM ZnSO4, 2 mM MgCl2, 2 mM Tris-HCl at pH 7.4, 100 mM KCl, and 10% glycerol) for 30 min. The protein/DNA solution was further mixed with glutathione-sepharose resin and incubated for 30 min. After incubation, the resin was washed three times with binding/washing buffer, and bound protein-DNA complexes were eluted with 10 mM glutathione solution. Libraries were constructed using KAPA Hyperprep kit (KAPA Biosystems),