GSM8136369: ParasymN_1; Homo sapiens; RNA-Seq
Day 30 parasymNs were dissociated using 0.25% Trypsin-EDTA (Gibco, 25200056) and 1mg/ml collagenase NB4 (Nordmark, S17454) for 40 minutes at 4 °C. Single-cell RNA-seq library was prepared using the PIPseqTM T20 3' Single Cell RNA Kit v4.0 from Fluentbio. A total of 40,000 cells were loaded for each PIP (Particle-templated instant partition) reaction.