16S/18S/ITS Amplicon Metagenomic Sequencing of Mus musculus: APCmin, WT
DNA was extracted by using Magnetic Soil and Stool DNA Kit, 16SrRNA/18SrRNA/ITS genes of distinct regions were amplified using specific primer with the barcode. Sequencing libraries were generated using NEB Next Ultra II FS DNA PCR-free Library Prep Kit following manufacturer's recommendations and indexes were added. The library was checked with Qubit and Real-Time PCR for quantification and bioanalyzer for size distribution detection.