GSM8408384: CD4+ Foxp3 eGFP+ TGFβ-iTreg cells, rep1; Mus musculus; RNA-Seq
Cells were lysed in 100 µl TRIzol (Invitrogen) and stored at -80°C before shipping them on dry ice to Single Cell Discoveries B.V. (Utrecht, The Netherlands). RNA extraction was performed using the standard protocol. Bulk-cell RNA sequencing was conducted using a modified CELSeq protocol (Hashimshony et al 2012;Simmini et al 2014).