GSM6449750: HGFs (human gingival fibroblasts ) treated with 5% Sg SCS replicate 2; Homo sapiens; RNA-Seq
Total RNA from HGFs was extracted after 24 hrs using the miRNeasy Mini kit and mRNA-seq was performed commercially (Novogen, Sacramento, CA). RNA degradation and contamination was monitored on 1% agarose gels, RNA purity was checked using a Nanophotometer® spectrophotometer, and RNA integrity and quantitation were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA). A total of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina, San Diego, CA) according to the manufacturer's instructions.