GSM7716284: NIL-qIGL1NIP rep1; Oryza sativa; RNA-Seq
Total RNA was isolated from young panicles (8---10 10-cm long) with Plant Total RNA Isolation Kit (Vazyme, China) according to the manufacturer's instructions.2 μg total RNAs were used for stranded RNA sequencing library. RNA sequencing library prepared by using KCTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on DNBSEQ-T7 sequencer (MGI Tech Co., Ltd. China) with PE150 model.