GSM7287594: Appl deleted, replicate 1, scRNAseq; Drosophila melanogaster; RNA-Seq
20 male and 20 female Drosophila brain of the appropriate geneotype were dissected on ice cold Schneider's medium. After a brief wash with PBS, the brains were incubated with 0.05% trypsin-EDTA (Fisher scientific) for 30 minutes, with continuous pipetting every 5 minutes. The dissociated tissue were filtered through a 10um cell strainer to get a single cell suspension. 10x libraries were constructed according to the manufacter's instructions (Chromium Next GEM Single Cell 3' Kit v3.1).