GSM6449793: UVA-dnCLIP-Ctrl-rep1; Homo sapiens; OTHER
Cells were lysed under 1% SDS and sonication. Cell lysates were diluted to capture MED23-RNA complex. The MED23-enriched RNAs were partially digested by RNase T1 (25 U/µl ), dephosphorylated by intestinal alkaline phosphatase and then phosphorylated by PNK. RNAs were extracted by TRIzol-chloroform-ethanol method. The enriched RNA fragments were subjected to small RNA library preparation with QIAseq miRNA Library Kits according to the manufacturer's instructions CLIP-seq