Gongxiaoshe 2013 sequence reads
DNA was extracted from 0.5 to 1 g (wet weight)of sediment and biomass-containing filters using a FastDNA spin kit for soil (MP Biomedical, OH, USA)according to the manufacturers instructions. DNA quality was assessed using a NanoDrop ND-1000spectrophotometer (NanoDrop Technology, USA). The extracted DNA was sheared to 500 bp using aCovaris Ultrasonicator (Covaris Inc., Woburn, MA, USA) following the manufacturers recommendation.The sheared DNA was then subjected to end pairing and to adaptor ligation with multiplexing and waspurified using an Ovation SP ultralow DR multiplex system.