Archaea 16S no FeCl2 treatment - microcosms-with methane and Fe
DNA was extracted using the DNeasy PowerSoil Kit (Qiagen Benelux B.V.). The samples were submitted to Macrogen (Seoul, South Korea) for amplicon sequencing of the V3 and V4 regions of the bacterial 16S rRNA gene useing primers Bac341F (CCTACGGGNGGCWGCAG) (Herlemann et al. 2011) and Bac806R (GGACTACHVGGGTWTCTAAT)(Caporaso et al. 2012). For Archaea 16S following primers were used: Arch349F (GYGCASCAGKCGMGAAW) (Takai and Hrikoshi 2000) and Arch806R (GGACTACVSGGGTATCTAAT) (Takai and Horikoshi 2000)