GSM7872955: NF-H3K27ac-2; Homo sapiens; ChIP-Seq
Cell preparation: Cell culture medium was removed by centrifuging at 300g for 5 minutes at 4°C. Cells were washed twice with 1 mL cold PBS and suspended in 60 μL PBS supplemented with 0.6 μL PIC and 0.6 μL PMSF solution. Cells were then stored on ice until used for ChIP-seq or RNA-seq. ChIP-seq library construction: MNase-digested chromatin samples of ~10,000 cells per library were used in MOWChIP-seq to profile H3K27ac. We prepared two ChIP-seq libraries and one input library for each condition. The protocol of MOWChIP-seq has been detailed previously (Zhu et al., Nature Protocols 14 (2019) 3366-3394.). ChIP DNA bound to the beads was collected by phenol-chloroform extraction. The Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences Cat# 21024) was used to prepare sequencing libraries.