GSM8085468: Peripheral blood_control_003; Homo sapiens; RNA-Seq
Single-cell suspensions were prepared from blood samples following red blood cell lysis using EasySep™ RBC Depletion Reagent. The cells were subject to a commercial Seq-Well platform, the Hive CLX gravity-based scRNA-seq 15,000 cells were loaded into the HIVE according to the capture protocol by centrifugation at 30 relative centrifugal fields (RCF) for 3 minutes. HIVEs were frozen at -20C until processing according to the updated HIVE scRNA-seq Processing Kit revA. Each cell was uniquely barcoded during the 3' cDNA library generation.