GSM8389806: Control,311,Passage2,20,rep_1; Homo sapiens; RNA-Seq
Total RNA was collected on day 8, 17, 32, 38 from NSCs/neurons, or from PS2, PS4 and PS8, after exposing cells to 0.1, or  20 ng/mL FGF2 for 6 days. RNA was collected from 3 different wells-replicates using RNeasy Mini Kit (Qiagen, 74104). mRNA was purified from 200 ng of total RNA with oligodT beads and sheared by incubation at 94°C in the presence of Mg (RocheKapamRNAHyper Prep Cat. KR1352).