GSM6451023: KPC biol. Rep. 2; Mus musculus; RNA-Seq
tumor cells and fibroblasts were extracted from solid tumors after digestion by cell sorting using flow cytometry. Tumor cells in ascites were isolated without digestion also by cell sorting with flow cytometry. Libraries and multiplexes were prepared with NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7600S) and NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, E6420S).