Transcriptome of Diphyllatea: Col17
Single Diphyllatea cells were isolated using a glass capillary micropipette. The cells were washed at least three times in sterile molecular grade water (Fisher Scientific), photographed when possible, and transferred to a 0.2 mL thin-walled PCR tube containing 2 L of cell lysis buffer (0.2% Triton X-100 and RNase inhibitor (Invitrogen)). cDNA was synthetized from pools of 3-7 cells, using the Smart-Seq2 protocol