RNA-seq of adenoid cystic carcinoma (ACC) ACCX11 cells compared to normal salivary glands
RNA-seq of adenoid cystic carcinoma (ACC) ACCX11 cells compared to normal salivary glands
Cells were detached by addition of trypsin. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. Total RNA was extracted using RNeasy Mini Kit (QIAGEN). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 1 ug (20 ng/Î¼l) of high-quality total RNA (using Monarch Total RNA Miniprep Kit according to manufacturer's instructions, New England Biolabs, Hitchin, UK) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-A enrichment. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.