GSM8408201: Motor cortex, Pgbd5-/-, RNA, rep 1; Mus musculus; RNA-Seq
Brains from 21 days old Pgbd5wt/wt (n=3) and Pgbd5-/- (n=3) littermates were extracted. Motor cortices were dissected, flash frozen and stored at –80 C. Nucleus extraction was performed according to the protocol from Masilionis et al (62) using the Singlulator100 (S2 Genomics). Extracted nuclei were stained with 7-aminoactinomycin D (Invitrogen) and FAC-sorted for further nuclei purification. Ten thousand nuclei were targeted for library construction using ChromiumNextGEM Multiome ATAC + Gene Expression kit (10xGenomics) Other: Single cell multiome ATAC + Gene Expression