GSM6628877: 12EAT-07-19_S12; Mus musculus; RNA-Seq
RNA was extracted using an OMNI Bead Ruptor and 2.8-mm ceramic beads (OMNI International) followed by Qiashredder and an RNeasy Lipid Tissue Mini Kit (for epididymal fat) using Qiacube (Qiagen) automated extraction according to the manufacturer's specifications with DNase treatment (RNase-Free DNase Set; Qiagen). Total RNA was quantified using QuantiT RNA Assay Kit (Thermo Fisher Scientific). Complementary DNA was prepared using qScript reverse transcription kit (QuantaBio). RNA libraries were prepared with the QuantSeq 39mRNA-Seq Library Prep Kit (Lexogen) for the Apollo 324 NGS Library Prep System and sequenced using Illumina NextSeq.