Single cell RNA-seq of cultured TIL: Sample TIL_12 week
10x Genomics Single Cell Gene Expression libraries were sequenced on a NovaSeq 6000 S2 and S4 run. All samples have sequencing yields of more than 23 million read per sample. The sequencing run was setup as a 26 cycles + 90 cycles non-symmetric run. Demultiplexing was done allowing 1 mismatch in the barcodes. The analysis was performed with the Cell Ranger 6.0.2 software using the default parameters.