snRNAseq of kidney of Pkhd1 null mouse model
Kidney from a 28-day-old Pkhd1wt/wt (control) mouse used for snRNAseq. Nuclei were isolated from a fresh 1-2 mm middle slice of the kidney spanning from cortex to inner medulla.  snRNA-seq libraries were obtained using 10X Genomics Chromium Single Cell 3 v3.1 chemistry following nuclear dissociation. Libraries were sequenced using Novaseq with an initial run with SP flow-cell followed by S4 flow-cell with rebalanced samples aiming for similar reads/cell number. Cellranger and standard 10x Genomics protocols  were used for initial processing