GSM7124049: Mut 0hr replicate 2; Homo sapiens; RNA-Seq
At select time points of interest, “chase” media was removed, and cells were lysed in using Trizol Reagent according to the manufacture's protocol with the following modifications: RNA isolation was performed in the dark and DTT was added to the aqueous phase to keep samples under reducing conditions. Library preparation was performed using QuantSeq 3' mRNA library prep kit for Illumina using the manufacturer's protocol and recommended maximum input amount