Illumina NovaSeq 6000 paired end sequencing; RNA-seq of perirenal fat from male and female Assaf suckling lambs
RNA-seq of perirenal fat from male and female Assaf suckling lambs
Perirenal fat samples were collected from 12 Assaf suckling lambs (6 males and 6 females). All animals were born at the Instituto de Ganadería de Montaña (IGM) (Grulleros, León, Castilla y León, Spain) in the same lambing season (winter 2019-2020) from primiparous Assaf dairy ewes. The lambs were reared under the same management and diet conditions. Initially, during the first 24 hours after lambing, the lambs were kept with their dams to suck the colostrum. Subsequently, the animals were held in lamb pens fed with artificial milk using Ovilac 60 (Calfvet®) milk replacer powder ad libitum up to slaughter to remove the influence of maternal effects. The lambs were slaughtered at a local slaughterhouse at the market weight for the  PGI label “lechazo de Castilla y Leon” (9-12 kg), which has average ages of 28.16 (±4.87) and 24.16 (±5.19) for males and females, respectively. The management, transport and sacrifice of the animals were in accordance with Spanish and EU legislation [Spanish Laws 32/2007, 6/2013 and RD 37/2014; Council Regulation (EC) 199/2009]. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue. For RNA extraction, the sampled tissues were preserved in an RNA-stabilization solution (Ambion RNAlater; Life Technologies) and stored at 4 °C for 24 h. Subsequently, the RNA-stabilization solution was removed, and the samples were frozen at -80 °C until RNA extraction. RNA was extracted using the miRNeasy Mini KIT (Qiagen, Germany) with adaptations for use in adipose tissue.