GSM7979050: 24h Induced BCBL-1; Homo sapiens; OTHER
Total RNA was isolated from cultured cells using the TRIzol reagent (Ambion) as per manufacturer's protocol. Poly(A)-tailed RNA was selected from 75 μg of each RNA sample using Dyna Beads mRNA purification kit (Invitrogen, Catalog No. 61006). Direct-cDNA sequencing libraries were prepared by using SQK-DCS109 (ONT) library preparation kit with 100 ng of each polyA-selected RNA sample.