GSM7928129: Mixed lymphocyte reactions, G22, scTCR-seq; Homo sapiens; OTHER
After 6 days of coculture, cells were harvested and stained with 7-AAD (BD Biosciences, Franklin Lakes, NJ, USA) and CD3 (Biolegend, San Diego, CA, USA). Viable CD3+Violet-lowCFSE-low cells (=proliferated recipient T cells) were then sorted using a FACS Aria II cell sorter (BD Biosciences). The Chromium Next GEM Single Cell 5' Reagent Kit V2 and the Chromium Single Cell Human TCR amplification Kit (10X Genomics, Pleasanton, CA, USA) were used for preparation of single-cell RNA and V(D)J libraries.