GSM7184270: ChIP-Seq B cells, untreated, anti-IKZF1; Mus musculus; ChIP-Seq
Immunoprecipitation was performed overnight at 4oC using antibodies to cJUN (Cell Signaling Technologies, 9165S), IKZF1 (Active Motif, 39291), normal mouse IgG (Santa Cruz, sc-2025), or normal rabbit IgG (Cell Signaling Technologies, 2729S). ChIP-Seq DNA libraries were prepared with the KAPA LTP Library Preparation Kit and barcoded with NEXTflex DNA barcodes, quantified, and sequenced on an Illumina HiSeq 3000 system. PCR products were barcoded (indexed) and sequenced on Illumina HiSeq 3000 platform.