GSM6593366: cortex_WT_Rep1; Mus musculus; RNA-Seq
RNA was isolated from the brain, spleen, kidney, heart, lung, and liver of 9-week-old WT mice and from the cerebral cortex and hippocampus of 14-week-old male Hs6st2 KO mice and their WT littermates. The Trizol reagent (Invitrogen, #15596026) was used to extract the total RNAs following the manufacturer's instruction. The Nanodrop spectrometer and the Bioanalyzer (Agilent 2100) using RNA 6000 Nano Chip were used to assess the quality, concentration, and integrity of the isolated RNAs. RNA samples with a RIN value of > 8 were included for the downstream experiments. RNA-seq libraries were prepared according to the manufacturer instructions. The Stranded Total RNA Library Prep kit (Zymo, #R3003) was used to generate RNA-seq libraries for the cerebral cortex samples, while the Invitrogen™ Collibri™ Stranded RNA Library Prep Kit for Illumina™ Systems (Thermo Fisher Scientific, # A38994096) was used to generate RNA-seq libraries for the hippocampus samples. The 1000 ng of total RNAs were used to prepare the RNA-seq libraries. The Bioanalyzer (Agilent 2100) using DNA 7500 chip was used to assess the quality of the RNA-seq libraries to make sure that the fragment sizes of all libraries were within the range of 300–500 bp. The Illumina NovaSeq sequencer was used to sequence the RNA-seq libraries.