GSM8085162: HCT116, shNC; Homo sapiens; OTHER
RNA was isolated after nuclear run-on reaction with biotin-11-CTP and biotin-11-UTP by biotin RNA enrichment process with magnetic streptavidin beads according to the PRO-seq protocol paper (Mahat et al., Nat Protoc, 2016) The isolated RNA was ligated with 3' and 5' RNA adapter and reverse transcribed and amplified by PCR and library size (140-350bp) was selected by Pippin Prep