GSM8223524: Xpo7KO, DMSO, RNA, rep2; Mus musculus; RNA-Seq
Cells were washed twice with ice-cold PBS, and total RNA extracted using the Rneasy Mini Kit (Qiagen). To enrich for mRNA, total RNA was processed using the Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). For RNA-seq using NpatKI AML cells, total RNA was prepared using the rRNA Depletion Kit v2 (New England Biolabs) to retain histone-derived RNA. Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer's instructions.