GSM8003716: SCR-3_1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Life technologies) according to the manufacturer manual. The RNA concentration and integrity were evaluated by NanoDrop 2000. TPCs derived from CRC patients with liver metastasis were infected with Guide RNA of NKX2-3 for 48 h and selected by puromycin (2 mg/mL).