GSM7290890: HA_NPCs_H4K91R_Input; Mus musculus; ChIP-Seq
Cells were fixed with 1% paraformaldehyde for 5 minutes with gentle rotation and then quenched with 125 mM glycine for 5 minutes. After cell lysis, chromatin was sonicated using a Covaris E220. Genomic DNA was isolated after the immunoprecipitation of sonicated chromatin. Libraries were generated using NEBNext Ultra II kits.