GSM7287537: 3X-TAM-3; Homo sapiens; RNA-Seq
Parental LNCaP cells and LNCaP MACC cells were stimulated with either vehicle (EtOH) or Tamoxifen (Sigma-Aldrich; cat. T176) and harvested for RNA-seq in biological replicates. Total RNA was extracted, and DNaseI treated by RNeasy Mini Kit (Qiagen; cat. 74104). Nanodrop quantified RNA was checked by Bioanalyzer RNA 6000 Nano Kit (Agilent Technologies). Samples with RNA integrity number > 10 were used for library preparation (Illumina Stranded mRNA Prep kit) and sequenced on Illumina NovaSeq 6000 at the WCM Genomics Core. RNA libraries for RNA-seq were prepared using Illumina Stranded mRNA Prep kit following manufacturer's protocols.