GSM6556245: C3H10_Ctrl,replicate 2; Mus musculus; RNA-Seq
C3H10 T1/2 cells were kindly provided by Stem Cell Bank, Chinese Academy of Sciences, and were cultured in MEM (Hyclone, SH30024.01) supplemented with 10% FBS and penicillin/streptomycin mix (50 mg⁄mL). Cells were plated at a density of 100000 cells/mL in 2 mL of medium on six-well flexible silicone rubber BioFlex culture plates coated with collagen type I (Flexcell International Corporation). The cells were cultured for 48 h, and then mechanical strain was applied. Cyclic mechanical strain (CMS) with a 0.5 Hz sinusoidal curve set at 10% elongation was applied for 8 h every day, with a FX-4000T™ Flexcell Tension Plus™ unit (Flexcell International Corporation, FX-4000T). The cultures were incubated in a humidified atmosphere at 37°C in 5% CO2 during stretching. Total RNA was extracted by RNeasy Micro Kit (Qiagen, 74004) according to the manufacturer's instructions. The cDNA was synthesized and amplified using the Single Cell Full Length mRNA-Amplification Kit (Vazyme, N712). The RNA-seq libraries were constructed from the amplified cDNA using the TruePrep DNA Library Prep Kit V2 for Illumina® (Vazyme, TD503). The PCR and size-selection of RNA-seq libraries were conducted as described in Hi-C library construction. The RNA-Seq libraries were sequenced on Illumina Nova Seq 6000 platform at PE150 mode.