GSM6908646: Mice-4-CD39pos; Mus musculus; RNA-Seq
RNA was isolated using a Single Cell RNA purification kit (Norgen), including RNase-Free DNase Set (Qiagen) treatment. The RNA integrity numbers were evaluated with an Agilent RNA 6000 pico kit. All samples were assessed according to the manufacturer's instructions. Reverse transcription and barcoded Illumina compatible libraries were generated from <10ng of DNA of each sample SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian (Takara) . Libraries were sequenced on an Illumina HiSeq - Rapid Run  using 100bp paired-end mode, 40 million reads per sample.