16S rRNA gene of anaerobic sludge : UASB bioreactor
V5-V6 region of the 16S rRNA gene using universal primer sets (515F (5 GTGYCAGCMGCCGCGGTAA -3) and 926R(5- CCGYCAATTYMTTTRAGTTT -3)). Sequencing was conducted using v3 Chemistry Kits (2  357 bp) on the MiSeq platform (Illumina, USA).