GSM6820459: 1897_LV_SingleDoseABE; Mus musculus; RNA-Seq
Single nuclei were obtained from flash-frozen tissues using mechanical homogenization folled by FACs sorting to remove debris. Nuclei were then loaded on the Chromium Controller (10X Genomics) with a targeted  nuclei recovery of 4,000–10,000 per reaction. Gene expression libraries were prepared according to the manufacturer's instructions of the v3 Chromium Single Cell Reagent Kits (10X Genomics).