GSM6690217: D108; Homo sapiens; miRNA-Seq
Total RNA was isolated using a mirVana™ miRNA Isolation Kit (cat. No. AM1561; Invitrogen), according to the manufacturer's protocol. Approximately 100 mg placenta tissue per sample was transferred to a Medicon disposable for biological sample disaggregation (cat. No. 340591; BD Biosciences, Franklin Lakes NJ, USA) containing 1 ml of lysis buffer (provided in the kit) and processed in a Medimachine (Dako; Jena, Germany) for 20 s. The small RNA libraries were created using Illumina's small RNA sample preparation protocol (TruSeq Small RNA Sample Prep Kits; Illumina, San Diego, CA, USA)