GSM7290687: pancreatic islets_Sorcs2-/-_sample 1; Mus musculus; RNA-Seq
Pancreatic islets from Sorcs2+/+ and Sorcs2-/- mice were isolated using collagenase dissociation method. Pancreatic islets were cultured overnight at 37°C and islets from 3 animals per genotype (~ 400-600 islets per sample) were collected in tubes. Single cell suspension was aquired by exposing pancreatic islet to Accutase with addition of 50 units/ml DNAse-1 for 10 min at 37°C. scRNA-seq libraries were generated using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10X Genomics Inc., USA). Briefly, a droplet emulsion targeting 10.000 cells was generated in a microfluidic Next GEM Chip G, followed by barcoded cDNA generation inside the droplets. Purified and amplified cDNA was then subjected to library preparation and sequenced on a NovaSeq 6000 instrument (Illumina, USA) to a depth of 40.000 mean read pairs per cell.