GSM7287598: HeLa, ANI ActD 1 h, rep1; Homo sapiens; RNA-Seq
RNA was extracted with the GeneMatrix universal RNA purification kit (Roboklon). Ribosomal RNA was depleted with the riboPOOL kit (Biozym, 27DP-K024). RNA-Seq libraries were prepared with the NEBnext Ultra II Directional RNA Library Prep Kit and sequenced single-end (81 nt) on a NextSeq550 device (Illumina).