GSM7184910: HSJD-DIPG-007 cells, vehicle treatment, day 0, repeat 1; Homo sapiens; RNA-Seq
Total cellular RNA was harvested with the RNeasy Mini Kit (Qiagen, 74104), including on-column DNase digestion with RNase-free DNase (Qiagen, 79256). RNA quality was assessed with an Agilent Tapestation 4150 (all samples had a RNA integrity number of >9.9). cDNA libraries were synthesised from 200 ng RNA using an NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB #E7760) according to manufacturer's instructions.