metagenomic sequencing of SDC-9 culture: suspended phase with biochar pyrolyzed at 500 celsius - bottle A
DNA was sheared with the Covaris E220 ultrasonicator (Covaris, Inc., Woburn, MA, USA) to an average size of 550 base pairs and indexed libraries prepared with the Roche Kapa HyperPrep kit. DNA libraries were sequenced on an SP Illumina Novaseq 6000 (2x150 bp PE) flow cell.