GSM7715398: ICSI_Co_twin_125b, blastocyst, scRNA-seq; Mus musculus; RNA-Seq
Single blastomeres or single blastocysts were lysed in 10X Lysis Buffer from Takara (cat.no. 635013). Then the RNA was extracted by TaKaRa SMART-Seq Single Cell Kit (cat.no. 634470). Total RNA was converted to cDNA using the TaKaRa SMART-Seq Single Cell Kit (cat.no. 634470) and sequencing libraries were prepared using the Illumina Nextera XT DNA Library Preparation Kit (cat.no. FC-131-1024). Libraries were sequenced on an Illumina NovaSeq 6000 platform.