GSM8408107: BaselinesnRNA6A; Homo sapiens; RNA-Seq
Bulk RNA-seq: Multiomic (snRNA-seq+snATAC-seq) Total RNA was extracted from sampled human brains using miRNeasy Mini Kite and homogenized in Qiazol using stainless steel beads. Chloroform was then used for phase separation. RNA containing an aqueous layer was further purified using the RNeasy MinElute spin column. RNA was finally eluted in RNase-free water.: Nuclei were isolated from brain tissue using the Chromium Nuclei Isolation Kit. Nuclei were directly processed for droplet capture for single cell multiome ATAC + gene expression using a chromium controller. For bulk RNA-seq librairies were prepared using Ribo-Zero gold sample preparation kit. Library concentrations were measured using KAPA Illumina Library Quantification Kit. Polled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry.: Chromium Next GEM Single Cell Multiome ATAC + Gene v1 chemistry was used to create single nuclei ATAC and RNA libraries from the same cell. Two baseline and two stimulated samples were used for independent replicates. Libraries were sequenced on an Illumina Novaseq 6000 with a 150 bp paired end read setup.