GSM6820386: Blastocyst, sgNprl2+sgTsc2 ovariectomy, E5.5, rep1 [sgN2T2_1]; Mus musculus; RNA-Seq
RNA was harvested using SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Japan)  and Total RNA Purification Kit, TRK1001 (LC Science, Houston, TX) cDNA was fragmented by dsDNA Fragmentase(NEB, M0348S) by incubate at 37°C for 30min.Library construction begins with fragmented cDNA. Blunt-end DNA fragments are generated using a combination of fill-in reactions and exonuclease activity, and size selection is performed with provided sample purification beads. An A-base is then added to the blunt ends of each strand,indexed Y adapters are ligated to the fragments, and the ligated products are amplified with PCR.