GSM8224425: TYUC1_Input; Homo sapiens; ChIP-Seq
Cells were digested with MNase to generate mainly mononucleosomes with minor fraction of dinucleosomes for histone modification mapping. Chromatin from 2 × 107 cells was used for each ChIP experiment, which yielded approximately 200 ng of DNA. Immunoprecipitation was performed using antibody against H3K27me3 (table S1) and Protein A Dynabeads (Invitrogen). DNA was purified using QIAGEN DNA Purification Kit. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's instructions and single-end 75bp reads were obtained using an Illumina NextSeq 500 sequencer.   Tracks were visualized using IGV.