GSM7843974: SNU-398 cells, overexpression EMX1-FL 1; Homo sapiens; RNA-Seq
In brief, RNA was extracted using TRIZOL/Chlorophorm/Phenol method. The Illumina RNA Library Prep kit (NEBNext® UltraTM) was used. The libraries were tagged with unique adapter indexes. Final libraries were validated on the Agilent 2100 Bioanalyzer, quantified via qRT-PCR, pooled at equimolar ratios, diluted, denatured, and loaded onto an Illumina novaseq 6000