Illumina HiSeq 2000 paired end sequencing; RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)
RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)
Hepatocyte-like cells (HLCs) were differentiated from hESCs or hIPSCs as previously reported (Hannah et al, 2013; Segeritz et al, 2018). Undifferentiated hIPSCs were maintained in Essential 8 medium.   RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Wellcome Trust Sanger Institute DNA Sequencing Facility (Hinxton, UK).