GSM8415183: CONT 53 PEAK; Homo sapiens; RNA-Seq
The isolated NK cells were washed with PBS and resuspended in Trizol, and RNA was extracted using the Trizol method. The RNA was then treated with DNase and purified using Zymo RNA cleanup columns. RNA concentration was measured with a Nanovue spectrophotometer, and RNA integrity was assessed using an Agilent Bioanalyzer, with RIN scores averaging above 8.0. Library preparations, RNA sequencing, and data processing were performed at the UC Irvine High Throughput Facility, with whole transcriptome sequencing conducted on an Illumina HiSeq platform (~200M X2 paired-end 100 bp reads).