wild-type_female_X_orco_male_GC1_replicate_3
Poly(A)-selected stranded libraries were prepared from 1 g total RNA with the Nugen Universal Plus mRNA-Seq kit (Nugen, Redwood City, CA, USA) with 7 cycles of PCR. Libraries were pooled and sequenced on a Novaseq 6000 across 2 lanes of an S4 PE150 flow cell such that each library was sequenced in both lanes.