RNA-Seq of HIEC-6 cells
total RNA using poly-T oligo-attached magnetic beads,Firststrand cDNA wassynthesized,PCR was performed with Phusion High-Fidelity DNA polymerase,PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system.The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v9-cBot-HS (Illumia) according to the manufacturers instructions.