GSM6593538: Cut&Run cJun_DMSO_rep2; Mus musculus; OTHER
FoxP3 YFP+ CD4+ T cells were FACS-purified Cut&Run: Cells were permeabilized using digitonin and incubated with anti-c-Fos, anti-c-Jun, or anti-NFAT2. MNase was activated to cleave and liberate the chromatin fragments bound by c-Fos, c-Jun, or NFAT2. Chromatin fragments were purified and amplified to generate libraries.