GSM8181477: naïve LNSCs_1; Mus musculus; RNA-Seq
LNs were harvested from C57BL/6 mice (8-12 weeks) and digested enzymatically. CD45- cells were purified with the CD45 negative selection beads and were stained with anti-mouse CD45 before sorting for viable CD45- cells. About 1.0 x  10^4 LNSCs were run on the  the 10X Chromium Controller (10X Genomics) to partition single cells into nanoliter-scale droplets containing uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 3' Reagent Kit (v3 chemistry). Generated cDNA libraries were sequenced on a NovaSeq 6000 sequencing system at the Institute for Genome Sciences in the University of Maryland, Baltimore.