Bacteria isolated from Harvard Forest soils
RC343 isolated in 2020 on Actinobacteria Isolation Agar with 100 mg L-1 cycloheximide. To extract DNA, the isolate were grown on 10% tryptone soya solid media at 30C shaking at 150 rpm until an OD of 0.5 was reached. gDNA was extracted by CTAB. Oxford Nanopore sequencing was performed at SeqCenter (Pittsburgh, PA) using R9 flow cells (R9.4.1)