GSM8085463: mBMSCs, All-EV; Mus musculus; RNA-Seq
RNA was isolated using the RNeasy Plus Kit (Qiagen) and 100 ng of total RNA used in making the sequencing libraries. mRNA samples from the mBMSC culture were sent to Azenta Life Sciences (South Plainfield, NJ) for next-generation sequencing. Briefly, RNA sequencing libraries were prepared from 100 ng RNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), The libraries were multiplexed and clustered onto a flowcell on the Illumina NovaSeq 6000 (San Diego, CA) and sequenced using an 2x150bp paired-end configuration. Approximately 20 million paired-end reads were sequenced per sample. Image analysis and base calling were conducted by the NovaSeq Control Software. Raw sequence data was converted into fastq files, de-multiplexed using Illumina bcl2fastq v2.20 software, and provided to us for analysis.