GSM7663178: FGF18 CTCF rep1; Homo sapiens; OTHER
All the Cut & Run experiments were performed using CUTANA Cut & Run prep Kit (14-1048, EpiCypher) according to the manufacturer's instructions. Cells were subsequently permeabilized with 0.01% digitonin and incubated with CTCF antibodies (EPR18253, Abcam) or H3K27me3 antibodies (C36B11, Cell signaling Technology) at 4℃ overnight. 0.5 ng of E. coli Spike-in DNA was added to each sample for normalization.  The K-MetStat Panel was spiked into H3K27me3 Cut & Run sample as an internal control. Library construction was performed using the CUTANA Cut & Run Library Prep Kit (14-1002, EpiCypher) according to the manufacturer's instructions. All libraries were amplified using 14 cycles on the thermocycler. The DNA fragments with target sizes ranging from 200 bp to 1000 bp were selected using AMPure XP beads (Beckman Coulter) and validated using the Agilent High Sensitivity DNA kit. OTHER: Cut & Run