GSM8003992: 297KB_PD1pos, scRNAseq; Homo sapiens; RNA-Seq
Enriched NK cells were stained with CD56-PE/Cy7, CD3-FITC, PD1-PE, CD19 and CD14-PB and viability dye (flour506-PB) and FACS sorted to obtain pure NK cells higher than 99%. NK cells were spun down and resuspended in growth media at 1000 cells/ul then sent to Genomics Birmingham-University of Birmingham to check the viability and sequencing . The generated library pool passed the QC in terms of concentration and fragment size as determined using Qubit and TapeStation and was sequenced using a NextSeq 500 (Illumina) platform. The median number of sequenced NK cells returned was 3,500 with 1,500 unique molecular identifiers (UMIs) per cell.Illumina NextSeq 500 (150 bps, paired-end) at a sequencing depth of >50,000 raw reads/cell. Illumina NextSeq 500 (150 bps, paired-end) at a sequencing depth of >50,000 raw reads/cell.