Illumina NovaSeq 6000 paired end sequencing; RNA-sequencing of forward programmed hepatocytes and fetal liver samples
RNA-sequencing of forward programmed hepatocytes and fetal liver samples
FoP-Heps were differentiated from hESCs by overexpression of a combination of 4 TFs (HNF1A, FOXA3, HNF6, RORc).  RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the NGS library facility of the Wellcome â€“ MRC Cambridge Stem Cell Institute (Cambridge, UK).