GSM7842778: Spinal cord tissue, SCI, rep3; Mus musculus; miRNA-Seq
RNA was harvested using TRlzol kit (B511311, Sangon, China). A total amount of 3 μg total RNA per sample was used as input material for the  small RNA library. RNA libraries for RNA-seq were prepared using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following  manufacturer's protocols.