GSM8085927: Ovary, WT, IR; Mus musculus; RNA-Seq
Cells were washed and suspended in PBS containing [0.04% BSA] and immediately processed as follows. Cell viability was assessed on a Countess II automated cell counter (ThermoFisher), and up to 12,000 cells were loaded onto one lane of a 10X Chromium X. Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform version 3.1 single-indexed chemistry and according to the manufacturer's protocol (#CG000204). Single cell capture, barcoding and library preparation were performed using the 10X Chromium platform version 3.1 single-indexed chemistry and according to the manufacturer's protocol (#CG000204).