Lab-built Landfill Microcosms
DNA sampling was conducted in an anaerobic chamber to maintain anaerobic landfill microcosm conditions. Community DNA was extracted with the QIAGEN DNAeasy PowerMax Soil Kit. Region-specific primers were used to amplify the V4 region (515F-806R) of the 16S rRNA gene by PCR. High-throughput amplicon sequencing was conducted on the Illumina MiniSeq platform at the Genome Research Core at UIC. Paired-end (150 x 2) libraries were built for each sample.