GSM7090762: Expanded_TIL_Feature_barcodes_4A; Homo sapiens; OTHER
Multimer binding CD8 T cells were enriched via FACS and brought to a concentration of 700-1,200 viable cells per microliter using a hemacytometer. These single cell suspensions were then loaded into the appropriate microfluidic chip (chip G; 10x Genomics) and run through a Chromium controller to obtain Gel Beads-in-Emulsion (10x Genomics). Resulting cell suspensions then went through a library preparation process for single-cell RNA sequencing (scRNA-seq) along with paired scV(D)J-seq for T-cell receptor (TCR) clonotypes using the 5′ transcriptome kit with feature barcoding (V1.1; 10x Genomics) following the manufacturer's guidelines. The complementary DNA libraries were then sequenced with 2 × 92 base pair paired-end reads aiming for an average of 5,000 reads per cell per library according to 10x Genomics guidelines.