RNA-Seq of Zootermopsis angusticollis: transcriptomic signatures of termite queens
Examination of gene expression patterns in worker (abdomen tissue without gut). Tissue was preserved in RNAlater (Qiagen) and kept at 4C for 24 h and then at -20C until further processing. Total RNA was isolated from thawed tissue samples with the peqGOLD Tri Fast Kit using chloroform, nuclease-free glycogen (5 mg/ml), Ambion isopropanol, and 75% ethanol. DNA was digested by DNase I recombinant (Roche) and EDTA (Sigma-Aldrich). cDNA libraries were constructed using the transcriptome library construction TruSeq RNA Library Prep Kit v2 (Illumina). HiSeq Transcriptome Sequencing was done on an Illumina HiSeq Xten platform (150-bp paired-end reads).