GSM7124365: B728a_P1; Pseudomonas syringae pv. syringae; RNA-Seq
Frozen samples were homogenized in 2mL homogenization tubes with high-density zirconium beads (Glen Mills) using a Geno/Grinder (SPEX SamplePrep) for 1 min at 1,750 Hz with a LN2 chilled sample holder. RNA was extracted using the DirectZol RNA miniprep kit (Zymo Research). RNA samples were DNAse treated with the Turbo DNAse (Thermo-Fisher) followed by cleanup with the Monarch RNA Cleanup Kit (NEB). Reagents were used according to the manufacturer's recommendations. Plant mRNA sequencing libraries were prepared with the Illumina-compatible KAPA Stranded mRNA-seq Kit (Roche) by the Georgia Genomics and Bioinformatics Core (GGBC). The Psy RNA samples were rRNA depleted using the RiboMinus Bacterial kit (Thermo Fisher) and libraries were prepared with the Illumina-compatible KAPA Stranded RNA-seq Kit (Roche) by the GGBC. The Pta RNA samples were rRNA depleted using both RiboZero Plant and RiboZero Bacteria (Illumina) in combination and RNA sequencing libraries were prepared in-house using the TruSeq Stranded Total RNA kit (Illumina). Library prep kits were used according to the manufacturer's recommendations.