GSM8389878: genomic DNA, Day 1, Rep 2; Caenorhabditis elegans; ATAC-seq
ATAC-seq was conducted as described in (Daugherty et al., 2017), using duplicates for every condition. In brief, day 1 adults were washed off the plates with M9, washed in PBS, and frozen in liquid nitrogen. Nuclei were released by mechanical homogenization(Haenni et al., 2012), counted by measuring optical density, and then processed as described in (Buenrostro et al., 2013). ATAC-seq libraries were constructed by following the protocol described in (Buenrostro et al., 2013). 10 ng of genomic DNA were used as the input control.