GSM7897998: Cut&Run H3K27Ac T58A replicate 1 spleen B cells stimulated for 48 hours with LPS; Mus musculus; OTHER
CUT&RUN was performed according to published methods using pA-MNase as secondary reagent. Yeast DNA was spiked into the samples after DNA extraction to normalize signal between samples. Cells were processed on an automated system at the Fred Hutch Cancer Center to carry out MNase cleavage, DNA extraction, and library construction. For CUT&RUN, cells were processed on a previously published, automated system at the Fred Hutch Cancer Center that uses KAPA DNA polymerase method for library preparation optimized for recovery of small DNA fragments.