GSM6559826: 11, EtOH, rep3; Drosophila melanogaster; ATAC-seq
Fat body was harvested by dissection using 25 flies per replicate, using only those flies whose ovaries displayed the proper response to S-methoprene supplementation or temperature effect. Flies with either S-methoprene or at 25C had large, viable ovaries, whereas flies at 11C with ethanol displayed the regressed ovarian morphology characteristic of diapause. Nuclear extraction and library preparation protocols were adapted from Cources et al., 2017 and Davie et al., 2015 using the omni-ATAC adaptation. The NEBNext™ Illumina library quantification kit from New England Biosciences was used to measure final library concentration (Ref # E7630S, lot 10013445). The Nextera™ DNA library prep kit from Illumina (Ref # 15028212, lot 20256848) was used to tagment DNA, ligate adapter sequences and amplify fragments. Adapter sequences used are available (Supplemental table X). The quality of ATAC-seq libraries was assessed via Bioanalyzer (Agilent) prior to sequencing.