GSM7927491: K562 cells, NES_TadA-8e expression,  treatment sample, poly(A) RNA-seq, replicate2; Homo sapiens; OTHER
Cells were lysed by Trizol (Invitrogen), and total RNA was extracted according to the manufacturer's instructions. Total RNA was treated by TurboDNase (Invitrogen) to remove DNA contamination. poly(A) RNA-seq library was constructed using VAHTS® Stranded mRNA-seq Library Prep Kit for Illumina® V2 (Vazyme, NR612, and N323). The total RNA-seq library was constructed using the same kit with the exclusion of the ploy(A) RNA selection step.