GSM7398597: NFYa cKO [atac]; Mus musculus; ATAC-seq
Hearts at E15.5 were dissected and flash-frozen in liquid nitrogen. Genotyping was performed to ensure proper pooling of hearts with the same genotype. Four hearts with the same genotype were pooled for each sample. Cardiac nuclei isolation was performed as previously described. Total cardiac nuclei were used to generate joint snRNA and snATAC libraries using Single Cell Multiome ATAC + Gene Expression (10×Genomics) according to the manufacturer's protocol. Sequencing was performed on an Illumina Nextseq 500 system operated by the Next Generation Sequencing Core of Children's Research Institute at UT Southwestern using the 150bp high output sequencing kit (Illumina).