GSM7123897: Human gut microbiota sample 4; human feces metagenome; OTHER
We collected approximately the size of chickpeas feces sample from 57 healthy Turkish volunteers,using a disposable spoon and transferred them into a screw cap tube containing 10 volumes of DNA/RNA Shield (ZymoResearch). We used the ZymoBIOMICS™ DNA Miniprep Kit (Zymo Researches, #D4300) to isolate bacterial DNA from the samples, following the kit's protocol. We prepared the sequencing library using the 16S Metagenomic Sequencing Library Preparation protocol from Illumina. The library had a size range of about 550 bp and a concentration of at least 10 nM. We sequenced 5ng of DNA from each sample using the iSeq 100 sequencing machine with paired-end (2x150) reading method.