msMRM_04_28
RT-DNA prep: DBR1 to remove the branched RNA from RT-DNA (if relevant), then extending the 3 end dCTP, in a reaction with terminal deoxynucleotidyl transferase (TdT); complementary strand synthesis from the extended product using Klenow Fragment with a primer containing an Illumina adapter sequence, six guanines, and a non-guanine (H) anchor. Finally, ligation of Illumina adapters at the 3 end of the complementary strand using T4 ligase