T-cell instructed monocyte activation is a key pathological feature in Ankylosing Spondylitis and provides novel therapeutic opportunities
T-cell instructed monocyte activation is a key pathological feature in Ankylosing Spondylitis and provides novel therapeutic opportunities
1M PBMC were cultured in 200 Î¼l medium in non-tissue culture treated 96-well round bottom plates (Corning) and left unstimulated or treated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16 h before being prepared for sequencing. After cell culture, cells were harvested and washed in PBS with 0.1% BSA. 20,000 cells from each condition were put into a channel of Chromium Next GEM Chip K of 10x Genomics platform. 10x 5' v2 10x 5' v2