GSM7661254: E12point5-FOLC-Lot-30; Mus musculus; RNA-Seq
Single cell suspensions were prepared of the day 6 aggregates (n=4 samples) and the thawed FOSC lots (n=3 samples) using trypsin EDTA (Gibco Cat#25300-054). Single cells were washed three to five times in cold PBS supplemented with 0.04% BSA. Single cell suspensions were processed to 10X Genomics GEM formation and library preparation using the Chromium Single Cell 3'Reagent Kits v3 (10X Genomics Cat# PN-100075). The libraries were made according to 10X Genomics protocols 10X Genomics