GSM7050763: F. nucleatum 3; Mus musculus; RNA-Seq
The experimental procedures were performed in accordance with the standard test procedures provided by Illumina. Total RNA was isolated and purified using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). The RNA integrity was assessed by Agilent 2100 with RIN number >7.0. Strain-specific library (mRNA-lncRNA-circRNA) was constructed by the method of removing rRNA depletion.