GSM7805795: HPC-Int H3K4me3; Homo sapiens; OTHER
Cells were prepared by removing MEF using a Feeder Removal Microbeads mouse (Miltenyi Biotec). Cells were incubated overnight with Concanavalin A beads and 1.3 µl of the primary anti-H3K4me3 antibody per reaction (Active Motif, catalog number 39159). After incubation with the secondary antibody (1:100), cells were washed and tagmentation was performed at 37℃ using protein-A-Tn5. Tagmentation was halted by the addition of EDTA, SDS, and proteinase K at 55℃, after which DNA extraction and ethanol purification was performed, followed by PCR amplification and barcoding