GSM8136371: RDY234 cells, control, repA; Saccharomyces cerevisiae; RNA-Seq
RNA purification and DNase I treatment were performed using a Quick-RNA Fungal/Bacterial Miniprep kit and DNase I set (Zymo Research) following the manufacturer  s recommendations with minor modifications. Reagents (RNA lysis buffer, RNA wash buffer, RNA prep buffer, 100% ethanol, and nuclease-free water) were supplemented with DTT to maintain reducing conditions. Samples were protected from light when possible.