GSM7124035: 12_NWC_S12_R1; Homo sapiens; miRNA-Seq
Total RNA was extracted from cells using Trizol Reagent (Thermo Fisher) according to the manufacture's protocol. Following phase separation, the aqueous phase was applied to a RNeasy Mini Kit (Qiagen) and RNA was purified according to kit instructions. Libraries were prepared using the QIAseq microRNA Library Kit from Qiagen. Libraries were prepared using the QIAseq microRNA Library Kit from Qiagen and sequnecing using Illumina NovaSeq SP.