GSM7764481: 50hpf; Danio rerio; RNA-Seq
Whole hearts were isolated and cardiac cells were dissociated. Dead cells were removed from the final cell isolate by FACs sorting in PBS without Calcium and Magnesium and with 0.04% BSA. Each sample was run separately on a lane in a Chromium controller with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3,1 (10xGenomics).  scRNA-seq library preparation was done using a standard protocol, and sequencing was done on a Nextseq2000