GSM7661036: mPO#2, differentiation condition, scRNAseq; Mus musculus; RNA-Seq
Organoids in the maintenance condition were dissociated by accutase incubation for 30-45 min with gentle pipetting every 10 min. Organoids in the differentiation condition were dissociated by the Papain Dissociation System for 30-45 min with gentle shaking/mixing every 10 min. Cell suspensions were passaged through 40 μm cell strainers to remove cell clumps. Library was used the 10X Genomics with Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 according to manufacturer's instructions.