GSM8389646: ADF8_ATAC_1h_rePi_rep1; Arabidopsis thaliana; ATAC-seq
Biotin tagged nuclei from ~100 mg from root tissue 1 cm above the root tip were captured on 25 uL of Streptavidin M280 Dynabeads according to the Isolation of Nuclei TAgged in specific Cell Types (INTACT) protocol. Bead bound nuclei for each timepoint in the starvation assay were counted in 1 mL of Nuclei Purification Buffer with 0.1% Triton X-100 (NPBt) and nuclei were split three ways to ~100,000 nuclei per sample in NPBt for three replicates. ATAC was performed in duplicate (~20k nuclei each) on these bead bound nuclei as previously described. (Identification of Open Chromatin Regions in Plant Genomes Using ATAC-Seq, Bajic M,Maher KA,Deal RB. Methods Mol. Biol.,2018)