GSM8408232: bnafah, SD1, rep2; Brassica napus; RNA-Seq
The total RNA was extracted using a TRIzol reagent (TIANGEN, Beijing, China) following the procedure of the manufacturer. Subsequently, the total RNA was qualified and quantified using a Agilent 2100 bioanalyzer. TruSeq RNA Sample Preparation kit v2 (Illumina, San Diego, CA, USA) was used for library construction with the instructions of the manufacturer. All the samples were sequenced using an Illumina HiSeq 2000 sequencer by Biomac Inc.(Beijing, China). The total RNA was qualified and quantified using a Agilent 2100 bioanalyzer. TruSeq RNA Sample Preparation kit v2 (Illumina, San Diego, CA, USA) was used for library construction with the instructions of the manufacturer.