GSM7843110: Mouse NK, Mef2c RNP, biol rep 3; Mus musculus; RNA-Seq
RNA was isolated from purified NK cells using the Quick-RNA Micro-prep kit (Zymo). Ribosomal RNA was depleted from samples using the QIAseq FastSelect -rRNA HMR kit (Qiagen). RNA libraries were prepared according to manufacturer instructions using the Stranded Total RNA Prep kit (Illumina, 20040525). Libraries were barcoded using the IDT for Illumina RNA UD Indexes Set A (Illumina, 20040553) and PCR-amplified libraries were cleaned up using Ampure XP beads (Beckman Coulter). Library quality and concentration were assessed by HSD1000 tape on 2200 Tapestation (Agilent) and Qubit 2.0 Fluorometer, and barcoded libraries were pooled to equimolar ratios for sequencing. Sequencing was performed on a NovaSeq 6000 system (Illumina).