WT-4W-2
Total RNA was extracted from WT (3-weeks) and rph1-1 (5-weeks) leaves using Trizol reagent kit (Invitrogen, Carlsbad, CA, United States), and rRNAs were removed by probe binding to the rRNA, the RNA purification with magnetic beads, to retain mRNAs and other RNAs. Enriched RNAs were fragmented and reverse transcribed into cDNA with random primers. The second stranded of cDNA was synthesized,  end repaired and ligated to sequencing adapters using T4 DNA ligase. After purification and fragment selection (300-500bp), the products were amplified and enriched by PCR. The sequencing library was sequenced on DNBSEQ-T7 with PE150 model at Bioyi Biotechnology Co.,Ltd. (Wuhan, China).