GSM7979094: BCBL-1_48h_induced_3KbPlus; Homo sapiens; OTHER
Total RNA was isolated from cultured cells using the TRIzol reagent (Ambion) as per manufacturer's protocol. Full-length cDNAs were prepared using Low Input RNA: cDNA Synthesis and Amplification kit (NEB, Catalog No. E6421) as per the manufacturer's guidelines. Full-length cDNAs were hybridized with KSHV baits to capture the KSHV specific cDNAs for preapring KSHV-Enriched libraries. cDNAs were size-fractionated and re-amplified. SMRTbell library adapters were ligated to cDNA fractions for preparing PacBio SMRT-Seq libraries.