RNA-Seq of Polygonatum odoratum Y4-1
1 g of total RNA from the rhizome of the A4 germplasm was then prepared for cDNA synthesis using a NEBNextUltra II RNA Library Prep Kit (New England Biolabs Inc., Ipswich, MA, USA) according to the previously published protocol. A Qubit 2.0 fluorometer (Life Technologies, CA, USA) and a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA) were used to verify the amount and purify of the library. Finally, the RNA was sequenced using a Illumina NovaSeq 6000 platform (Illumina) supplied by Shanghai OE Biotech Co., Ltd. (Shanghai, China).