RNA-seq of Klebsiella pneumoniae SGH10 grown in LB or DMEM medium
RNA-seq of Klebsiella pneumoniae SGH10 grown in LB or DMEM medium
SGH10 bacteria were grown under DMEM (Dulbecco's Modified Eagle Medium) and LB(Lysogeny broth).  Bacteria were harvested by centrifugation.  The bacterial pellet was resuspended in TE buffer, mixed with lysosyme (SigmaAldrich) in a final concentration of 0.9 mg/ml, and immediately frozen in liquid nitrogen. The frozen pellet was subjected to two cycles of thaw and freeze at 37Â°C and in liquid nitrogen, respectively, and RNA was extracted using Tri-Reagent (Sigma-Aldrich) RNAtag-seq with mudifications detailed in Melamed et al, Nature Protocols 2018