GSM7873080: Unstimulated 2h - Unstimulated 24h [26_2h_medium_24h_medium]; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Mini Kit (74106, Qiagen), including an on-column DNase digestion (79254, Qiagen) Total RNA samples having RIN values>8 were subjected to xGen™ mRNA library preparation, according to the manufacturer's instructions (xGen™ RNA Lib Prep 96rxn, CAT 10009814) Stranded mRNA libraries were analyzed on a 2100 Bioanalyzer instrument, following the manufacturer's protocol “Agilent DNA 7500 kit” (G2938-90024, Agilent Technologies), diluted to 10nM and pooled equimolar into multiplex sequencing pools for sequencing on NovaSeq 6000 instrument (Illumina).