GSM7898477: OVCAR8ip1 cells, Control _2, biol rep 2, 48h; Homo sapiens; RNA-Seq
Total RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research) according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.