GSM8414566: WGBS_Exp2_KO_plus_EV 3aKO BM Primary; Mus musculus; Bisulfite-Seq
For RNA sequencing, 4000 HSC cells were sorted using Aurora cell sorter. And RNA was extracted using NucleoSpin RNA plus XS (Takara: 740990.50) . For WGBS, genomic DNA (gDNA) was prepared from the cells using PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific #K1820-02). RNA librariers for RNA seq was preapred using Takara Clontech SMARTer mRNA library kit following manufactures protocol. For WGBS, using 200 ng of gDNA and 0.2% lambda DNA was bisulfite treated using EZ-96 DNA Methylation-Gold Mag Prep Kit (Zymo Research #D5042) and processed with xGen™ Methyl-Seq Library Prep (IDT, 10009824) to generate Illumina-compatible WGBS libraries.