GSM8004590: E15.5Heart, HighFatDiet, Rep1; Mus musculus; ATAC-seq
To isolate the nuclei for ATAC-seq, embryonic heart tissues were first rinsed with ice-old PBS. Nuclei were then extracted using a dounce homogenizer in ATAC lysis buffer. ATAC-seq libray preparation was performed using the ATAC-seq kit (Active motif, 53150) in accordance with the manufacturer's instructions.