GSM6625132: E13.5 p27 positive progenitors, Sox4 ChIP, replicate2; Mus musculus; OTHER
cochlea ducts were dissected at  E13.5 from p27kip1-GFP  mice and epithelial layer was isolated using enzymatic digestion with 0.25% dispase (Gibco) and collagenase I (Gibco) for 10 min. Cells are then dissociated and FACS sorted to obtain GFP positive cochlea epithelail progenitor cells. CUT&RUN libraries were constructed using Accel-NGS 2S plus DNA prep kits with single index and MIDs (Swift Bioscience). At least 20 million paired-end reads were sequenced for each sample.