NextSeq 500 paired end sequencing; RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)
RNA-sequencing of hepatocyte-like cells (HLCs) and primary human hepatocytes (PHHs)
Primary human hepatocytes were sourced from Biopredic International as monolayer plated cultures (pPHHs), or freshly isolated (fPHHs) from tissue donors as previously reported (Segeritz et al, 2018). RNA was extracted using the GenElute Mammalian Total RNA kit (Sigma). Library preparation was performed by the Cambridge Genomic Services (Cambridge, UK).