GSM7842665: Lymphoma patient 3 CD8+ CAR T cells (separate) (day 14) [Sample_2154-S02_sep_CD8]; Homo sapiens; RNA-Seq
Total RNA was isolated from FACS-sorted CD8+ CAR T cells using the Qiagen RNeasy kit. Total RNA integrity was assessed using an Agilent 4200 TapeStation (Agilent) and quantified using a Trinean DropSense96 spectrophotometer (Caliper Life Sciences). RNA-seq libraries were prepared from total RNA using the TruSeq RNA Exome library prep kits (Illumina) (day 8 samples) or SMART-Seq v4 Ultra Low Input RNA Kit (Clontech) and the Nextera XT DNA Library Preparation Kit (Illumina) (day 14 samples). Library size distribution was validated using an Agilent 4200 TapeStation. Additional library QC, blending of pooled indexed libraries, and cluster optimization was performed using a Qubit 2.0 Fluorometer (Life Technologies-Invitrogen). RNA-seq libraries for samples 1 through 12 were prepared from total RNA using the TruSeq RNA Exome library prep kits (Illumina, Inc., San Diego, CA, USA) following the manufacturer's protocols. RNA-seq libraries for samples 13 through 24 were prepared from total RNA using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech Laboratories, Inc., Mountain View, CA, USA) and the Nextera XT DNA Library Preparation Kit (Illumina, Inc., San Diego, CA, USA) following the manufacturer's protocols.