GSM6448299: 24h APF wing, H3K27me3, 20 wings, supernatant; Drosophila melanogaster; OTHER
Tissues were dissected in CUT&RUN wash buffer, then dissociated in collagenase/dispase solution for 40 minutes Libraries were prepared using Takara ThruPLEX DNA-seq and Dual Index kits. Libraries were amplified using 14-19 PCR cycles, then cleaned up using AMPure XP beads from Beckman Coulter. Library quality was assessed using an Agilent Tape Station.