GSM6449787: WT-FIP1-1; Homo sapiens; ChIP-Seq
1 x 107 cells were collected and crosslinked in 5 ml PBS with 1% formaldehyde for 10 min at room temperature. After quenching and cell lysis, chromatin was sonicated using a Covaris M220 sonicator. VRC (Sigma, 94742) at a final concentration of 2 mM was added to the cell lysis buffer, nuclear lysis buffer and IP buffer after sonication to prevent RNA degradation. ChIP was performed using 10 µg of an anti-FIP1 antibody . The ChIP and input libraries were constructed with a VAHTS Universal DNA Library Prep Kit (Vazyme, ND607) according to the manufacturer's instructions. Libraries were amplified by distinct PCR cycles to obtain 200 ng final DNA libraries.