Microbial diversity of the anodic biofilm during zinc recovery using a two-chamber microbial electrolysis cell.
PCR amplification was performed using Platinum II Taq Hot-Start DNA Polymerase (Thermo Fisher Scientific), as follows: initial DNA denaturation step at 94 C for 3 min, 35 cycles of DNA denaturation at 94 C for 45 s, annealing at 52 C for 60 s with a 50% thermal ramp, extension at 72 C for 90 s, and a final extension step at 72 C for 10 min. The library was purified by AMPure XP beads (Beckman Coulter) and sequenced using a MiniSeq System (Illumina) with MiniSeq Mid Output Kit (300 cycles).