GSM6381675: Col_G24h_2; Arabidopsis thaliana; ATAC-seq
Seeds at different germination stages or 10-day-old seedlings were chopped in lysis buffer (15 mM Tris-HCl pH 7.5, 20 mM NaCl, 80 mM KCl, 0.5 mM Spermine, 0.2% Triton X-100, 5 mM β-ME, protease inhibitor cocktail). After being filtered with a 30μm filter (CellTrics), nuclei were stained with DAPI and subjected to fluorescence-activated cell sorting (FACS). 50,000 nuclei per sample were collected and mixed with Tn5 transposase (Mei5bio, MF650-01), after 20 minutes of incubation at 37°C, tagmented DNA was recovered with ChIP DNA Clean & Concentrator kit (ZYMO, D5205). After PCR amplification, sequencing libraries were purified with VAHTS DNA Clean Beads (Vazyme N411)