GSM7660846: Small2; Mus musculus; RNA-Seq
For RNA extraction, tumors were excised, snap-frozen in liquid nitrogen, and homogenized in TRIzol. RNA was extracted and purified using an RNeasy Mini Kit. mRNA was enriched using the NEBNext Poly(A) mRNA Isolation Module where each sample had an RNA integrity number (RIN) score of >7. A NEBNext Ultra II Directional RNA Library kit was used for RNA-seq library construction.