GSM8182192: Fib-EV 1; Homo sapiens; miRNA-Seq
Total RNA from EVs was isolated using miRNeasy Serum/Plasma Kit (QIAGEN) according to the protocol. The quantity and quality of total RNA were assessed using the Agilent 2100 Bioanalyzer (Applied Biosystems, Carlsbad, CA). The NEBNext Multiplex Small RNA Library Prep Set for Illumina was utilized to prepare a small RNA library. The qPCR-based KAPA Biosystems Library Quantification kit (Kapa Biosystems, Inc., Woburn, MA) was used to determine precise measurement for sequencing purposes.