GSM6777840: S1-N, adjacent normal tissues (RNA-seq); Homo sapiens; RNA-Seq
Frozen tissues were extracted using the QIAamp Fast DNA Tissue Kit (Qiagen) for DNA.The experimental process was performed according to the standard protocol provided by Oxford Nanopore Technologies (ONT), including sample quality detection, library construction, library quality detection and library sequencing. DNA-seq libraries were captured to exome regions using Roche NimbleGen SeqCap EZ Exome V3/Agilent SureSelect Human All Exon V6. RNA was subjected to library preparation using ONT Direct RNA Sequencing Kit. Libraries were sequenced on PromethION.