SELEX pool 8 R1
For deep sequencing of the entire selection process using Next Generation Se-quencing (NGS), the individual rounds of SELEX were prepared as follows. For ampli-fication of double stranded DNA (dsDNA), 8 l of the RT-PCR product of each individual round was mixed with 20 l 5X Q5 reaction buffer (NEB), 200 M dNTPs, 0.5 M ol-igonucleotide pool_fwd (5'-CCAAGTAATACGACTCACTATAGGGCAACTCCAAGCTAGATCTACCGGT-3'), 0.5 M oligonucleotide pool_rev (5'-AGTGAAAAGTTCTCCTTTGCTAGCCATTTT-3'), 2 U Q5 high-fidelity DNA polymerase (NEB) and ultrapure water (filled up to 100 l). After performing PCR (30 s at 98C, 5 cycles of {10 s at 98C, 20 s at 55C, 20 s at 72C}, 2 min at 72C), gel electrophoresis was performed to exclude overamplification. Subse-quently, the samples were purified using Promega's Wizard SV Gel and PCR Clean-Up system. The concentrations were determined by spectrophotometric measurement using a NanoPhotometer (IMPLEN) and adjusted to 20 ng/l with ultrapure water. Illumina sequencing was performed by GENEWIZ (Azenta Life Sciences).