GSM6502698: Individual 3, Skin biopsy-derived fibroblasts, small RNA; Homo sapiens; ncRNA-Seq
For polyA RNA-Seq, total RNA was extracted from HeLa cells using the Maxwell RSC simplyRNA Cells Kit (AS1390, Promega), and from patient-derived fibroblasts using the Maxwell RSC miRNA from Tissue Kit (AS1460, Promega), according to the manufacturer's specifications. For small RNA-seq total RNA was extracted from HeLa cells and patient-derived fibroblasts using the Maxwell RSC miRNA from Tissue Kit (AS1460, Promega) according to the manufacturer's specifications. For polyA RNA-seq, stranded poly-A selected mRNA libraries were constructed from 1 µg total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England Biolabs) and NEBNext Ultra II Directional RNA Library Prep for Illumina with Sample Purification Beads (E7765, New England Biolabs). Amplification was performed using 8 cycles of PCR to avoid over-amplification. Unique dual-indexed barcode adapters were applied to each library. Small RNA-seq library construction was carried out by BGI Genomics (Shenzhen, China).