GSM7184533: Human primary keratinocytes, treated 1µM S1P 24 h, rep2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the Truseq Stranded mRNA Kit and IDT for Illumina Truseq RNA UD Indexes from Illumina, following manufacturer's protocols.