GSM7287588: GFP-positive neurons, Ethanol, P15, replicate2; Mus musculus; ATAC-seq
Tagmented DNA was cleanup with a DNA Clean and Concentrator-5 kit (Zymo Research, cat# D4004). Cleanuped DNA was amplified by PCR with index primers for 20 cycles. Purified libraries with Ampure XP (Beckman Coulter, cat# A63880) were sequenced with NovaSeq 6000 System (Illumina) as 2 x 50 bp paired-end readings.