GSM8222817: IκB– recip, MPP3, rep2; Mus musculus; ATAC-seq
Bone marrow from the bilateral femur and tibia was collected; red blood cells were lysed and mature hematopoietic cells were depleted using magnetic bead-based lineage antibody cocktail.  Pre-selected cells were then flow sorted to collect donor HSPC subsets: LT-HSC (CD45.1+/Lin– /Sca1+ cKitHi/Flt3–/CD150– CD48–), MPP2 (CD45.1+/Lin– /Sca1+ cKitHi/Flt3–/CD150+ CD48+), MPP3 (CD45.1+/Lin– /Sca1+ cKitHi/Flt3–/CD150– CD48+) and MPP4 (CD45.1+/Lin– /Sca1+ cKitHi/Flt3+). Between 500-1500 cells were collected per sample and processed for ATAC-seq as described in Buenrostro et al, 2013 using Tn5 transposase from Illumina. Libraries were prepared with ATAC-seq with Nextera DNA Library Preparation Kit (Illumina, FC-121) according to manufacturer's instructions.