GSM6373847: Timeseries_TTchemseq_SOX2-FKBP_1H_Rep1; Mus musculus; OTHER
Libraries for TTchemseq were prepared following a published protocol(Gregersen et al., 2020). Cells  were labeled with 2mM 4SU for 10 minutes. Total RNA was isolated and  fragmented. The 4SU-biotin labeled RNA was enriched using  streptavidin coated MicroBeads. Libraries were prepared using KAPA  RNA HyperPrep kits (Roche) using dual indexing adapters.