GSM7763770: Gadd45β KO mice lung, 0 day bio rep 2; Mus musculus; RNA-Seq
Total RNA was extracted from whole lungs of 6-8 weeks old female Gadd45β+/+ and Gadd45β-/- mice, with 2 biological replicates per group obtained using the NucleoSpin RNA extraction kit (MACHEREY-NAGEL) according to the manufacturer's instructions. The quality and integrity of total RNA samples were assessed using the Agilent 4200 TapeStation (Agilent technologies) according to the manufacturer's instructions. cDNA library was generated using the QuantSeq 3' mRNA-Seq library prep kit FWD for illumuna (Lexogen)