GSM8003998: nPOD6292, snRNA-seq without endocrine population enrichment; Homo sapiens; RNA-Seq
Nuclei were extracted from frozen human pancreata using GRO-seq or citic acid method. For those experiment with FANS, nuclei were  labeled with the primary antibody against NKX2-2 (DSHB,  74.5A5, 1:100) and  Cy3 donkey-anti-mouse secondary antibody (Jackson ImmunoResearch, 715-165-151, 1:200). Sorted nuclei were loaded onto a 10x Genomics Chromium Next GEM Chip G (10X Genomics, PN1000127) and library preparation was perfrmed according to the manufacturer's protocol (Chromium Next GEM Single Cell 3' reagent kits v3.1 (dual index)).