GSM7123638: WT; Mus musculus; RNA-Seq
collagenase P was perfused along the common bile duct of the mice to fill the pancreas after mice were dissected, followed by digestion in a 37 ℃ water bath, and Hank's Balanced Salt Solution containing 10% FBS was added to terminate the digestion. Then the islet cells were picked under a stereomicroscope. In order to obtain single cell suspension, the selected islets were digested with trypsin and passed through a 40um single-cell sieve, and the single cells were finally used for single-cell sequencing. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.