FLAG-MyoD1 ChIP-seq of FLAG-MyoD1-overexpressed B7 on day 3
ChIP-seq was performed by fixing cells with 1% formaldehyde, followed by lysis and fragmentation by Branson sonicator. Fragmented DNA was incubated with target antibody overnight. After de-crosslinking, ChIP DNA was purified and library was constructed by Thruplex DNA-seq.