GSM8136514: control microvessel, day 6, young serum exposure, round 2; Homo sapiens; RNA-Seq
Microvascular models were washed with 1x PBS and perfused with RLT buffer; downstream lysate was collected and supplemented with β-mercaptoethanol, before isolating RNA using a RNeasy Mini Kit (Qiagen) with DNase I digestion (Qiagen). RNA libraries were prepared with the Takara SMART-seq v4 kit following QC of purified lysates.