GSM8004974: KO-Contrl2; Mus musculus; RNA-Seq
12-week-old age- and sex-matched Stat1-/- and wild type mice were intratracheally inoculated with 5x103 CFU K. pneumoniae clinical isolate W42 grown to mid-log phase. After 24 hours the mice were euthanized by an overdose of isoflurane. Uninfected age and sex matched naïve mice served as untreated controls. Whole mouse lungs were digested with collagenase A and DNAse I then filtered through 70mm sterile cell strainer to generate single cells suspensions. Neutrophils were depleted using Anti-Ly-6G MicroBeads (mouse) from Miltenyi Biotec. Cell viability was checked by Cellometer Auto 2000 Automatic Cell Viability Counter system (Nexcelom). library was performed according to the manufactere's instructions (single cell 5'V2 protocol, 10x Genomics).