GSM6625019: LNCaP_WT_shCtrl_noDOX; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with streptavidin magnet beads pull-down. The extracted ChIP DNA was ligated to specific adaptors using KAPA LTP Library Preparation Kit (KK8230) or KAPA Hyper Prep Kit (KK8504), according to the manufacturer's instructions.