GSM7843954: MC38-Ctrl xenograft; Mus musculus; RNA-Seq
After the xenograft was removed, it was digested into single cell suspension with tumor tissue dissociation kit on ice, and 10x-RNA reagent was added. Single-cell suspensions were resuspended in 0.04% BSA in PBS after digestion, the concentration was at least 400 cells/μL. Single-cell RNA-sequencing libraries were prepared by the Chromium Single Cell 3′ reagent kit v2 (10X Genomics, Pleasanton, CA) according to the manufacturer's protocol. In brief, the cells were diluted into the Chromium Single Cell A Chip to yield a recovery of around 6000 single-cell transcriptomes with < 5% doublet rate, then the libraries were prepared. The libraries were sequenced in multiplex on the NextSeq 500 sequencer (Illumina, San Diego, CA). Sequencing data were processed with the Cell Ranger version 3.0.1 (10X Genomics, Pleasanton, CA).