GSM7844001: DpnII Hi-C on HeLa S3 MR DMSO or ICRF-193 + Merb added at t2: t0 Mitotic R1; Homo sapiens; Hi-C
For each Hi-C library, approximately 1-5 million cells were used as input. Cells were crosslinked for 10 minutes at room temperature in a final concentration of 1% formaldehyde. Glycine was added to quench crosslinking and pellets were frozen at -80C until Hi-C library generation. Hi-C was performed as described in Belagzhal et al, 2017, Methods. Restriction enzyme: DpnII