GSM8390058: Klhdc3ko_r3_F01; Mus musculus; RNA-Seq
RNA was extracted using the RNeasy Mini Kit by Qiagen and was quantified using Nanodrop. 500 ng of total RNA was used for the construction of the sequencing library. The library was prepared using Alithea Genomics BRB-Seq (MERCURIUS™ BRB-seq Library preparation kits for Illumina®) in a 96 well plate format) following the manufacturer's protocol. Quality was pre-checked using RNA ScreenTape Assay (TapeStation) and Qubit.