AMPLICON 16S Sequencing of peat metagenome
SAMPLE COLLECTION: ~12 ml of LifeGuard buffer (Qiagen) (LG) was added to the tube, followed by vigorous hand shaking to achieve a thorough mixture of LG and peat. NUCLEIC ACID EXTRACTION: Standard (S) - a modified Mobio PowerMax Soil DNA/RNA isolation kit (Qiagen Cat# 12966-10). This protocol has been used from 2010-present for multi-omics studies by several sequential US-based projects (IsoGenie: DOE grant #s DE-SC0004632, DE-SC0010580, & DE-SC0016440; and EMERGE: NSF grant # 2022070).