Growth and gene expression changes in Pseudomonas alloputida KT2440 in response to light and heavy lanthanides
Growth and gene expression changes in Pseudomonas alloputida KT2440 in response to light and heavy lanthanides
Biomass was harvested from mid-exponential cultures by means of centrifugation. Pseudomonas alloputida KT2440 was grown using MP medium to mid-exponential phase, using phenylethanol (5 mM) as carbon and energy source. Cultures were grown with different lanthanide elements (La, Nd, Er, a lanthanide cocktail [Ce, Nd, Dy, Ho, Er, Yb]; 50 nM) or without lanthanide addition. Total RNA was extracted with TRI reagent (Applied Biosystems), and mRNA was enriched using the RiboMinus bacteria 2.0 transcriptome isolation kit (Invitrogen). Sequencing libraries were prepared with the NEBNext Ultra II Directional RNA library prep kit for Illumina (New England Biolabs).