GSM6625128: E13.5 cochlea, whole organ, wildtype, replicate3; Mus musculus; ATAC-seq
cochlea ducts were dissected at  E13.5 from wildtype mice and epithelial layer was isolated using enzymatic digestion with 0.25% dispase (Gibco) and collagenase I (Gibco) for 10 min. Whole epithelia were lysed in the buffer containing 10mM TrisHCl (pH=8.0), 5mM MgCl2, 10% DMF, 0.2% NP40 in ddH2O. Transposition buffer containing 10 mM Tris-HCL (pH=8.0), 5 mM MgCl2, 10% DMF, 0.2% NP40, and Tn5 transposes was then added and incubate at 37°C for 20 min. After DNA purification, amplification and library construction was performed through PCR.