GSM8004109: ATAC_WT_bio_1; Arabidopsis thaliana; ATAC-seq
About 1 g of 14-day-old Arabidopsis seedlings grown under short-day conditions were collected and extract the nuclei. The purified nuclei were then incubated with Tn5 transposome and tagmentation buffer at 37°C for 30 min (Vazyme Biotech, Cat. No. TD501). After the tagmentation, the DNA was purified using MinElute PCR purification kit (Qiagen, Cat. No. 28004) and then amplified using TruePrepTM DNA Library Prep Kit V2 for Illumina® (Vazyme Biotech, Cat. No. TD501) for 9 cycles.