GSM6820353: MCF-7_ZNF652_ChIPSeq; Homo sapiens; ChIP-Seq
Lysates were clarified from sonicated nuclei and ZNF652-DNA complexes were isolated with antibody. All DNA samples were processed as Illumina ChIP-seq sample processing methods. 10 ng of ChIP DNA was end polished with T4 DNA polymerase and kinase. An 'A' base was added to the polished DNA fragments, followed by the Qiaquick column clean up.After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp were electively isolated from the 1% agarose gel and eluted by the Qiagen gel extraction kit. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.  Lysates were clarified from sonicated nuclei and ZNF652-DNA complexes were isolated with antibody. All DNA samples were processed as Illumina ChIP-seq sample processing methods. 10 ng of ChIP DNA was end polished with T4 DNA polymerase and kinase. An 'A' base was added to the polished DNA fragments, followed by the Qiaquick column clean up.After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp were electively isolated from the 1% agarose gel and eluted by the Qiagen gel extraction kit. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina HiSeq 2500 following the manufacturer's protocols.