Pacbio HiFi reads for montezuma quail genome assembly
The gDNA was sheared with a Megaruptor 3 to an average fragment length of 13Kb. "Sheared gDNAs were converted to a library with the SMRTBell Prep kit 3.0. The library was sequenced on 1 SMRTcell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 30hs movie time. CCS analysis was done in instrument with SMRTLink V11.1 using the following parameters: ccs --min-passes 3 --min-rq 0.99