GSM8222625: K562_Nucleus_rep1; Homo sapiens; RNA-Seq
RNA was extracted from subcellular fractions with Trizol LS according to the manufacturer's protocol. Synthetic yeast RNA spike-ins were added. RNA libraries for RNA-seq were prepared using the SMARTer Stranded Total RNA HI Mammalian kit (Takara 634873) with 1 µg of RNA as input according to the manufacturer's protocol. For Nuclear Wash RNA without rRNA depletion (NW_NRRD) samples, the rRNA depletion step was omitted from the manufacturer's protocol.