GSM6373738: 12_CD33_123; Homo sapiens; RNA-Seq
The total RNA from 12 samples (triplicates of OCI-AML3 wt, -CD33KO, -CD123KO and -CD33/123KO) was isolated according to RNeasy kit manufacturer's instructions (QIAGEN). The RNA quantity was assessed using NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific) and Qubit 2.0 Flurometer (Life Technologies), while RNA Integrity Number (RIN) values were evaluated by microfluidic electrophoresis on a BioAnalyzer 2100 (Agilent Technologies). The RIN value score was ranging from 9 to 10. The 12 mRNA-libraries were prepared according to NEBNext Ultra II Directional's manufactures instruction kit with PolyA selection