GSM8224303: SVI-003-21 Unsorted; Homo sapiens; RNA-Seq
Isolated human pancreatic islets were stained with antibodies against LY6H and sorted by FACS using a BD Influx (BD Biosciences) or BD FACSAria Fusion (BD Biosciences). RNA extraction of FACS purified populations was performed using Trizol reagent as directed by the manufacturer (ThermoFisher, 15596026). RNA libraries were prepared for sequencing using a Truseq Stranded mRNA Library Prep Kit (Illlumina; Donor SVI-015-20) or using a SMARTer Stranded Total RNA-seq Kit v3 - Pico Input Mammalian (Takara; Donor SVI-003-21) as per the manufacters instructions.