GSM8003811: After purification [BC2]; Influenza A virus (A/Puerto Rico/8/1934(H1N1)); OTHER
DNA fragments were extracted using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific) For before library, randomized sequences were cloned into pCIG3 vector. The vector were then electropotated into competent cells and plasmid DNA was extracted and used as template to prepare samples. For after purification library, gDNA was extracted from sorted cells. Sequences was cloned into a soluble hemagglutinin expressing vector based on pDZ vector. Sequencing sample was prepared as described previously.