GSM7842941: MDA-MB-436_siRNACTRL_plate_biol rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using the RNeasy kit (#74104, Qiagen) according to the manufacturer's instructions. For Plate-seq, cells were lysed in Buffer TLC (Qiagen) supplemented with 2-mercaptoethanol and processed for high-throughput mRNA sequencing in the PLATE-seq assay RNA quality was assessed on an Agilent Bioanalyzer instrument using Agilent RNA 6000 Pico Reagents. RNA-seq libraries were prepared with the Illumina TruSeq Stranded Total RNA Sample Preparation kit. For Plate-seq, cells were lysed in Buffer TLC (Qiagen) supplemented with 2-mercaptoethanol and processed for high-throughput mRNA sequencing in the PLATE-seq assay Final libraries were quantified with the KAPA Library Quantification Kit (Roche), pooled in equal amounts and sequenced (PE150) on an Illumina HiSeq4K sequencer. For Plate-seq, cells were lysed in Buffer TLC (Qiagen) supplemented with 2-mercaptoethanol and processed for high-throughput mRNA sequencing in the PLATE-seq assay