Whole genome sequencing of Ormosia species
(1) Fragmentation of genomic DNA;(2) End repair of fragmented double-stranded DNA;(3), the "A" base was added to the 3 'end of the DNA fragment;(4) Connect the two ends of specific sequencing adaptor DNA fragments;(5) Purify the ligation product to remove the unligated adapter sequence;(6) PCR amplification of fragment DNA connected to adaptors and purification;(7) Sample library detection;(8) Sample library, hybridization, capture of hybridized target DNA fragments and separation and purification, removal of unbound DNA fragments and digestion of RNA;(9) PCR amplification of captured DNA fragments and purification of PCR products;(10), capturing the resulting sample library assay.