RNA-seq of inner ear RNA from mice carrying a mutation in Zfp719 and wildtype controls at 3 different ages
RNA-seq of inner ear RNA from mice carrying a mutation in Zfp719 and wildtype controls at 3 different ages
Reference for mice used is MGI ID 4431958. 4, 14 and 21-day old mice from heterozygote x heterozygote matings were collected and culled by decapitation (for 4-day old mice) or cervical dislocation (for 14- and 21-day old mice).  The head was bisected and the brain removed. For P4, the inner ears were dissected out in RNAlater; for P14 and P21, the inner ears were isolated from the temporal bones before moving into RNAlater, then further dissected in RNAlater to get rid of extra tissue attached to the inner ears. The inner ears were transferred into eppendorf tubes (without RNAlater) and dropped into liquid nitrogen. Samples were stored at -20 or -80 degrees Celsius. Pestles were used to break down the inner ears, then RNA was extracted using the QIAgen RNeasy kit with QIAshredder columns. Strand-specific libraries were prepared using the Illumina TruSeq stranded mRNA LT kit.