GSM8222577: WT_ovaries_1_8st_BEAF32_ChIP; Drosophila melanogaster; ChIP-Seq
For the ChIP-Seq experiments ovaries were collected in PBS buffer (50 pairs per ChIP), cross-linked with 1% of formaldehyde for 5 min and then incubated for 5 min with 125 mM Glycine. After that ovaries were washed with PBS buffer for three times. The remaining ChIP protocol was performed as described previously (Vorobyeva et al (2013) NAR, 41:11, 5717–5730). ChIP-Seq libraries were prepared for sequencing using standard Illumina protocols