GSM7927797: E11.5 Wild Type Dental Lamina 8; Mus musculus; RNA-Seq
Tissues were dehydrated through a graded ethanol series, embedded in paraffin wax and sectioned (7 μm). Selected regions of tissue sections were microdissected with the Leica LMD7000 Laser Microdissection System, at 20× magnification while keeping laser power to a minimum. After cells were collected in cap, tubes were spun down quickly and 5 μl of Smart-seq2 lysis buffer (with 0.5% Triton X-100, 5μg Proteinase K and 2 U μl−1 RNase inhibitor) was added, followed by pipetting up and down to mix. The Smart-seq2 protocol was followed for cDNA preparation, Tn5 Tagmentation, and library preparation for next generation sequencing. Quality of cDNA and sequencing libraries were determined using a Bioanalyzer system (Agilent) and real time PCR. The KAPA Library Quantification Kit (Roche) was used for quantification of RNA sequencing libraries.