MeRIP-Seq data for cell models and gliomaspheres expressing either IDH1-Mut or IDH1-WT
MeRIP-Seq data for cell models and gliomaspheres expressing either IDH1-Mut or IDH1-WT
Patient brain tumor samples were collected during surgery and glioma stem cells were purified from the samples. Glioma cells were made to grow into gliomaspheres in a growth factor cocktail containing media, prior to centrifugation and nucleic acid extraction. Total RNA was extracted and purified from cellular samples using TRIzol Reagent (Ambion, Cat# 15596018), while mRNA was purified using PolyATract mRNA Isolation Systems Kit (Promega, Cat#Z5300) from total RNA samples. RNA transcripts were fragmented into ~100 nucleotide segments and isolated for m6A enrichment using m6A Dynabeads (Synaptic Systems, Cat# 202003) for each sample. This allowed for input RNA and m6A enriched RNA to be analyzed following MeRIP-Seq using RADAR. Stranded mRNA Library Preparation Kits (Illumina, Cat# 20020594) were used for sequencing library preparations, with unique adapter indices ligated to each sample to allow for multiplexing (TruSeq RNA Single Indices Set A, Illumina, Cat# 20020492).