GSM8224919: BRCAB1-3G; Mus musculus; RNA-Seq
Cells were counted on the ADAM automated cell counter and viability was estimated with the AccuStain solution kit (NanoEntek). Samples were labeled and multiplexed by group, allowing to be pooled in a single GEM for encapsulation. Cell labeling was performed according to the Cell multiplexing oligo labeling protocol from 10x Genomics (CG00391). libraries were generated using the Chromium Next GEM Single Cell 3' Library and Gel beads kit v3.1 according to the manufacturer's instructions