GSM6560256: SHEP, H3K27me3, ABCAM, control2; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes and quenched with 0.125 M glycine. After cell lysis, chromatin was sonicated using a Diagenode Bioruptor (30 seconds on and 30 seconds off) for 10 minutes in precooled conditions. Lysates were pelleted at 4˚C and the supernatant was collected. DNA was purified using Qiagen PCR kit (Qiagen 28104). ChIP DNA libraries were made using Ovation® Ultralow System V2 kit (Tecan Genomics 0344NB-32).