GSM8075299: macrophage, uninfected with ZRM at 4h, Rep3; Mus musculus; RNA-Seq
TRIzol reagent was added to the macrophages at 4 hpi and 24 hpi, the TRIzol extractions were collected, and centrifuged to remove debris and pellet Mtb. RNA was isolated and purified using the PureLink RNA mini kit (ThermoFisher). Ribosomal RNA was depleted from the samples using Ribo-Zero plus rRNA depletion kit (Illumina®, Cat. #:20037135) before being sequenced on an Illumina® HiSeq platform for generation of 150 base pair paired end reads. RNA sequencing (RNAseq) was performed by GENEWIZ® (South Plainfield, NJ 07080).