GSM8085148: KPS2.SIY replicate 3; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen, Cat#15596026) following the manufacturer's instruction. The aqueous phase containing the RNA was then purified using the RNeasy MinElute Cleanup Kit (Qiagen, Cat# 74204) according to the manufacturer's instruction. NEB polyA prep using 250 ng input and 1 to 5 adaptor dilution with 14 cycles of amplification