GSM6448760: K562_sgBCR-1_NA_NA_rep3; Homo sapiens; RNA-Seq
1 million live cells were sorted using a BD FACSAria and placed on ice. Immediately following the sort, cells were centrifuged, pelleted, and flash frozen in liquid nitrogen and placed at -80C. Pellets were processed in groups of 24 using the Zymo RNA Miniprep Kit and quantified using the Invitrogen Quant-it RNA kit. RNA was then diluted to a concentration of 10 ng/ul and 10 ul of each sample was transferred to a 96 well plate. Poly-A enrichment and library construction were performed using the NEB Ultra II RNAseq kit, and paired-end sequencing was performed on an Illumina NovaSeq S4.