GSM6381805: Control AntagomiR experiment 2 [CANTR2]; Ovis aries; RNA-Seq
Total RNA was harvested using Trizol, and then shipped on dry ice to Novogene for sequencing. mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads. Purified mRNA was fragmented, and first strand cDNA was synthesized with random hexamer primers and followed by second strand cDNA synthesis. Libraries then had end repair, A-tailing, adapter ligation, size selection, amplification, and purification and were then ready for quality control. Libraries were checked for quality, quantity, and size distribution with Qubit and RT-PCR. Libraries were then pooled and sequenced on an Illumina NovaSeq 6000 platform.