RNA-seq of iPSCs
  200-500k cells from each of the iPSC lines and differentiated lines were treated with Trizol for lysing. RNA was extracted using a Zymo Direct-zol RNA Microprep kit (Zymo Research, R2060). 200 ng of RNA from each sample was used to generate RNA-seq libraries using the NuGEN Universal Plus mRNA-Seq + UDI kit (Tecan, 0520). The libraries underwent 2x75 paired end sequencing by Illumina NextSeq 500.