DNA amplicon seq of rattus norvegicus : adult male caecal content
DNA extracted from 100-200 mg faeces or luminal content using Qiagen Powersoil DNeasy kit. DNA concentrations measured using Qubit 2.0 dsDNA HS kit (Qiagen) and adjusted to 5 ng/L before PCR. V3-region 16S rRNA genes was sequenced using the Ion Torrent GSS5 platform in DTUs DMAC core as previously described (Christensen et al. 2014).