GSM6629063: Lungs, Wildtype, 2 months, Rep2; Mus musculus; RNA-Seq
Lungs were perfused with chilled PBS to remove the blood. Lung tissues were then homogenised into cell suspension in  Trizol reagent using gentleMACS dissociator. After a series of washing, RNA pellet was dissolved in RNase free water. 1.5 ug of total RNA was used for the construction of sequencing libraries. RNA library is formed by polyA capture (or rRNA removal) and reverse transcription of cDNA. Illumina PE150 technology is employed to sequence the sample and the final stage involves the bioinformatics analysis.