GSM7805879: AVE0991-1; Mus; ncRNA-Seq
Total RNA was extracted from the right SNpc tissues of mice using TRIzol reagent (Thermo Fisher Scientific) and its concentration and quality were measured by Qubit fluorometer (Thermo Fisher Scientific) and 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), respectively. The quality of data processing of raw reads was checked by FastQC-v0.11.8, and low-quality bases and adaptors contamination were trimmed with Fastp-v0.19.5 if necessary. RNA library preparation was performed according to the manufacturer's instructions, using 1 μg of DNase-treated total RNA as input. The libraries were quantified by using an ABI 9700HT Fast Real-Time PCR system (Thermo Fisher Scientific).