Eukaryotic 18S Metabarcoding Analysis of water from the Clinch River, Virginia, USA, incubated in a fish tank with Actinonaias ligamentina for 24 hours  replicate 3
10 Liters of water were removed from the Clinch River and incubated in a 10 gallon fish tank for 24 hours with the Unionid mussel Actinonaias ligamentina . 1 Liter of water was passed through a 0.45 micron filter. DNA was extracted from the microbes caught on the filter using Qiagens Power Water kit. 18S eukaryotic barcodes were PCR amplified. Illumina sequencing, including library preparation, was completed by Azenta (South Plainfield, NJ, USA).