GSM6448788: ER_cst8644_E2wd24; Mus musculus; OTHER
Cells were stained with a final concentration of 1 ng/ml DAPI in growth media, and 1 million live cells were sorted using a BD FACSAria and placed on ice. CUT&RUN was then performed using the Epicypher CUTANA CUT&RUN kit, using the indicated number of cells, antibody, and concentration of antibody. Library construction was performed using the NEBNext Ultra II Library Preparation Kit following the indicated protocol, and each library was indexed using NEBNext® Multiplex Oligos for Illumina. For PU.1, the library construction protocol was modified to generate shorter fragments per https://www.protocols.io/view/library-prep-for-cut-amp-run-with-nebnext-ultra-ii-wvgfe3w. Libraries were quantified using Qubit and Bioanalyzer and pooled for sequencing using an Illumina NextSeq 500. OTHER: CUT&RUN