WGS of mixed population
The DNA was extracted and prepared as described previously (14), and was used to prepare sequencing libraries using Illumina's Nextera Flex DNA library prep kit. Sequencing was performed on the Illumina NovaSeq platforms using Paired-end 150bp reads at Charles River Laboratories, Victoria, Australia. The reads were processed to contain only bases above Phred quality score of 25 using Trim Galore v0.6.13