AMPLICON 16S Sequencing of peat metagenome
SAMPLE COLLECTION: Sample was immediately flash-frozen in liquid nitrogen (LN). NUCLEIC ACID EXTRACTION: Standard with alternative reagents (SR) - Identical to the 'S' method, except that the reagents used in the original kit were replaced with identical alternative reagents provided by Mobio/Qiagen. Specifically, RD1 was replaced with PM1, RD2 with C3, RD3 with PM4, RD4 with C5, and RD5 with DEPC-treated water.