GSM8222773: MV-4-11 cells, 2 days, SAHA_100nM_rep2; Homo sapiens; RNA-Seq
Total RNA was isolated from cell pallets using RNAiso Plus reagent (Takara) and 1 μg total RNA was used to generate cDNA with the PrimeScript™ RT Master Mix (Takara). RNA was quantified with NanoDrop followed by integrity evaluation via Agilent 5400 TapeStation analysis according to manufacturer's protocol. RNA libraries for RNA-seq were prepared using mRNA-Seq library kit following manufacturer's protocol.