GSM8084968: MGG8 scRNAseq; Homo sapiens; RNA-Seq
MGG8 cells were dissociated as described, washed and dead cells were removed using the dead cell removal kit (Miltenyi Biotech, #130-090-101). Briefly, cells were incubated for 15min at room temperature with dead cell removal microbeads, then diluted in 1X binding buffer and loaded in the column. Effluent contained live cells and the column was washed with 1X binding buffer in order to maximize efficiency. Live cells were centrifuged, resuspended in 1X PBS BSA 0.04%, counted and resuspended at 106 cells/ml. Chromium Single Cell 3' v3.1 Library and Gel Bead kit (PN-1000121) and Chromium Next GEM Chip G Single Cell Kit (PN-1000127) were used according to the manufacturer's instructions. 3000 cells were loaded on the chip, then 10X capture and library protocol was used without modification and samples were sequenced on the Illumina Novaseq 6000