GSM7806283: 8cell embryos FLI1; Sus scrofa; RNA-Seq
Total RNA was isolated from 2,4,8 cell embryos DMEM /FLI experimented group (each of three replicates) using a Micro kit (Thermo Fisher, AM1931) according to the manufacturer's instructions. The amount of RNA was measured by a Qubit 2.0 Fluorometer with Qubit™ RNA High Sensitivity kit. SMARTer Stranded Total RNA-Seq Kit v2-Pico Input Mammalian Kit (Takara, France) was used for RNA library construction. Libraries quality and quantity were assessed using Bioanalyzer 2100 with a High Sensitivity DNA assays kit and a Qubit dsDNA High Sensitivity kit. Libraries were sequenced on NovaSeq 6000 according to the manufacturer's protocol using NovaSeq 6000 SP Reagent kit (200 cycles) using a mode for reading at the end of pairs with a length of reading 2 x 100 base pairs (bp).