RNA-Seq of hypoxic preconditioned bone marrow-derived mesenchymal stem cells: hypoxic preconditioning
Library construction utilized the TruSeq RNA kit (Illumina, USA): total RNA was purified, fragmented, and used for cDNA synthesis. Double-stranded cDNA was prepared by end repair and addition of A bases. The cDNA libraries were purified, PCR enriched, quantified, and sequenced on the NovaSeq 6000 Instrument (Illumina, USA) with read length PE150.