GSM6448349: HD 2, PBMC, eTconv, bulk RNA-seq; Homo sapiens; RNA-Seq
To construct RNA-seq libraries, starting with 100 pg of total RNA, the cDNA was synthesized using a SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio USA, Mountain View, CA) according to the manufacturer's instructions. One nanogram of amplified cDNA was used to prepare the RNA-seq libraries for Illumina sequencing. The libraries were constructed with a Nextera XT DNA Sample Preparation kit (Illumina, San Diego, CA) according to the manufacturer's instructions.