GSM7897861: IMR-90, Pro, input; Homo sapiens; ChIP-Seq
Cells were crosslinked with 1% formaldehyde for 10 min at room temperature. Following cell lysis, cells were treated with 10 U of Micrococcal nuclease per 1 × 10^6 nuclei at 37℃ for 20 min and then sonicated by Picoruptor (Diagenode) for 2 min. Chromatin fragments were incubated at 4℃ overnight with the appropriate antibodies, followed by a pull-down assay using M-280 sheep anti-rabbit IgG (Thermo Fisher Scientific). After decrosslinking, the DNA was purified using a QIAquick PCR Purification Kit (Qiagen). The ChIP-seq library was prepared using a NEBNext Ultra DNA Library Prep Kit and sequenced using the Illumina NextSeq 500/550 system (Illumina), according to the manufacturer's protocol.