GSM6594243: SiScd1-3_L2_1; Mus musculus; RNA-Seq
Embryo were lysied firstly and cDNA was made with Superscript II reverse transcriptase. The cDNA was amplified with KAPA Hifi HotStart using ~12 cycles. Sequencing libraries were constructed from 1 ng of preamplified cDNA using a DNA library preparation kit (TruePrep DNA Library Prep Kit V2 for Illumina, Vazyme). Libraries were sequenced on a HiSeq PE150 according to the manufacturer's instructions.