GSM6556228: COH-B6 1; Mus musculus; OTHER
Fecal pellets were collected aseptically and placed in sterile eppendorf. Immediately freezing stool in liquid nitrogen (LN) and store at -80°C. Extract stool bacterial DNA followed by QIAamp Fast DNA Stool Kit  protocol For the illumina library construction, 16S rRNA was amplified by using primer set  Forward Primer:5'- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3 and Reverse Primer:5'- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3' Library Preparation: 16S Metagenomic Sequencing Library Preparation-Nextera XT DNA sample preparation