GSM7123406: Run 1 UOK262 Mutant RNA-seq--Replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using Trizol Reagent according to the manufacture's protocol. Following phase separation, the aqueous phase was applied to a Qiagen RNeasy Mini Kit column and RNA was purified according to kit instructions. RNA-seq libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit v2 and sequenced on NextSeq 500 using paired-end sequencing