GSM7660710: Lung, D2, i.n. boost; Mus musculus; RNA-Seq
Lungs, spleen, and MLN were harvested from mice that received DNA-OVA i.m. prime plus LAIV-OVA i.n. boost vaccination at designated time points. Red blood cells were removed, and total single cells were collected and proceeded to the dead cell removal (following the instruction of Dead Cell Removal Kit, Miltenyi Biotec). 2×104 live cells were subsequently proceeded to the downstream scRNA-seq library construction. Library was performed according to the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index), 10X genomics.