RIL-seq analysis of MG1655 Escherichia coli experiencing long-term nitrogen starvation in Gutnick minimal media
RIL-seq analysis of MG1655 Escherichia coli experiencing long-term nitrogen starvation in Gutnick minimal media
Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115 Wild-type and Hfq::3XFLAG E.coli were grown in Gutnick minimal media with 3mM NH4Cl as the sole nitrogen source, and cell samples were taken during exponential growth (N+), ~20min following N-runout and induction of growth arrest (N-), 24hr following N-runout and induction of growth arrest (N-24) and 2hr following addition of NH4Cl to N-24 cells (N-24+2). Samples were prepared according to the protocol found in this paper: doi:10.1038/nprot.2017.115. Final RNA purification by TRIzol extraction Preparation of strand specific cDNA performed by Vertis Biotechnologies. cDNA synthesis was conducted with oligonucleotide (dT)-adapter primers and M-MLV reverse transcriptase, followed by PCR amplification using TruSeq-designed primers from Illumina guidelines.