GSM8084898: 4349, AMLN, semaglutide_high; Mus musculus; RNA-Seq
Lysis of tissue was performed using a MP FastPrep system. Briefly, 15-20 mg liver biopsy was homogenized and used for RNA extraction on NucleoSpin Plus RNA columns (Macherey-Nagel) as recommended by the supplier. The quantity of the RNA was assessed using a NanoDrop 2000 spectrophotometer (ThermoScientific). The purified RNA was stored at -80c NeoPrep™ Library Prep System