GSM6507412: H1299 cells, Bro40mut-2, 24 hours; Homo sapiens; RNA-Seq
RNA was harvested using Trizol (Thermo Fisher Scientific), followed by chloroform phase separation, isopropanol precipitation and ethanol dehydration. 1 ug of total RNA was used for the construction of sequencing libraries. Sequencing libraries were generated using KAPA mRNA HyperPrep Kit (KAPA Biosystems, Roche, Basel, Switzerland) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.