Fungal ITS  Metabarcoding Analysis of water from the Clinch River, Virginia, USA replicate 3
1 Liter of water was removed from the Clinch River and passed through a 0.45 micron filter. DNA was extracted from the microbes caught on the filter using Qiagens Power kit. ITS fungal barcodes were PCR amplified. Illumina sequencing, including library preparation, was completed by Azenta (South Plainfield, NJ, USA).