NextSeq 500 sequencing; Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery
Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery
Primary neuron cultures were prepared from C57B/6 wild type mice (P0) pups and cultured as previously described (Kaech and Banker, 2006). RNA was extracted from Trifast lysate and alkylated using SLAMseq Kinetics Kit (Lexogen 062.24). Libraries were prepared with the Truseq stranded mRNA library prep kit (Illumina 20020594) according to the manufacturerâ€™s recommendation. Libraries were prepared in triplicates (biological replicates).