GSM8085169: INPUT SCAN1#1 U2OS Rep2; Homo sapiens; OTHER
Non-crosslinked nuclei were isolated and genomic nucleic acids were extracted and sonicated. IPs were performed with the S9.6 antibody. IP and Input samples were fragmented and libraries were performed using the NEBNext UltraTM II DNA Library Prep for Illumina (NEB) from 10 ng of samples. For IP samples only, a RNase H treatment to degrade the RNA in the RNA-DNA hybrids was performed before synthesis of second strand cDNA with dUTP mix.