GSM8085486: PBMC from BatchB (9 hashed donors, chip1 lane8) during ART, GEX; Homo sapiens; OTHER
PBMC from 38 participants were washed then individually hashed with TotalSeq-C anti-human hashtag antibodies (BioLegend). Uniquely-hashed participant PBMC were pooled into 4 batches of 9 or 10 participants and cells from each batch were loaded into 4 different wells of a Chromium chip (10x Genomics), with targeted recoveries of 16,000 cells/well, for subsequent library construction. Library constructions were processed for CITE-seq using the Chromium Next GEM 5' Single Cell V(D)J Reagent Kit with Feature Barcode v1.1 (10x Genomics) per manufacturer's instructions.