GSM8181751: Input, mouse cardiomyocytes, PBS treatment, GapmeR negative control, replicate 1; Mus musculus; ChIP-Seq
Briefly, ~2 million cardiomyocytes were crosslinked with 1% formaldehyde for 15 minutes at room temperature. Then, the chromatin was fragmented using a QSonica Q800R3 sonicator to obtain an average chromatin length of 300 nucleotides. The fragmented chromatin was incubated with H3K27me3 (CST, #9733) or H3K9me3 antibody (CST, #13969) at 4 °C overnight. The antibody-bound chromatin was immunoprecipitated using protein A/G agarose beads (Thermo Fisher Scientific, #20421). ChIP-seq library preparation and NGS sequencing were carried out by Novogene Corporation Inc., with at least 50 million tags sequenced for each library using 150 bp pair-ended sequencing.