GSM7287032: KO replicate 3; Mus musculus; RNA-Seq
Cells were lysed in buffer RLT (Qiagen) and DNAse-treated RNA extracted using the Qiagen RNeasy Miniprep kit with on-column DNAse. Quality of RNA was confirmed by RIN using a fragment analyzer (Agilent) poly-A stranded RNAseq libraries were generated from raw RNA Libraries were pooled and sequenced on a NovaSeq with 100bp paired-end reads to a depth of approximately 120 million reads/sample