GSM7843961: Input pTRIPZ RACK1, mutant RACK1 Replicate 1; Homo sapiens; RNA-Seq
For performing RNA-seq, input lysates from the samples subjected to Ribo-Seq was used.  Briefly, the cells were plated, grown, and subjected to the various treatments and experimental conditions indicated as described above.  They were the rapidly harvested and lysed.  Clarified cell lysates was used for RNA-seq library preparation. Total RNA was isolated using the Direct-zol RNA kit (Zymo Research) according to the manufacturer's protocol.  After rRNA reduction using the riboPOOL rRNA depletion kit (siTOOLs Biotech, H. sapiens pool), the RNA was then used to generate strand-specific RNA-seq libraries TrueSeq Stranded Total RNA Library Prep (Illumina, 20020596).  The RNA-seq libraries were subjected to QC analyses (final library yield, and the size distribution of the final library DNA fragments) and sequenced using an Illumina HiSeq 2000. rRNA depleted-RNA-seq