GSM7898578: ELP1_1h_A; Homo sapiens; OTHER
Total RNA was extracted using phenol:chloroform method. Wild-type, ELP1-KO and CTU1-KO HEK 293T cells were grown in 100 μM 4-thiouridine (Tocris) for 1h or 2h and harvested by adding 300 μl Trizol (Invitrogen) directly to the dishes. RNA was isolated, treated with iodoacetamide (Merk) and sequencing libraries generated using QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina (Lexogen) according to the manufacturer's instructions.