GSM7844331: human_skin_3; Homo sapiens; RNA-Seq
Single nuclei were isolated with Minute Single Nucleus Isolation Kit for Tissues/Cells (Invent Biotechnologies, Inc, Minnesota). The tissue was minced into pieces and lysed in cold lysis buffer, lysate was filtered with centrifuge filter, cell pellet was resuspended in PBS containing 5% BSA. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). single cell 3' v3 protocol, 10x Genomics