GSM8136790: scRNAseq Serum+/KD shRNA1, replicate2; Homo sapiens; RNA-Seq
For ChIP-seq, brain organoids were fixed with 1% formaldehyde at room tempture for 10 minutes. Cells lysis and chromatin fragmentation were performed using SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology, 9005). For scRNA-seq, single nuclear were prepared following the standard protocol of Chromium Nuclei Isolation Kit with RNase Inhibitor (10X Genomics, PN-1000494). For ChIP-seq, libraries were prepared using NEBNext UltraTM II DNA Library Prep Kit for Illumina (NEB, E7645S). For scRNA-seq, library preparation was preformed following the manufacter's protocol of Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10X Genomics, PN-1000268).