GSM7661182: Control_rep2_Poly; Homo sapiens; RNA-Seq
Total RNA was extracted from control or SMYD5-depleted KKLS cells using Trizol reagent. Polysomal RNA was extracted from pooled fractions indicated as heavy polysomes. Both total RNA and polysomal RNA were subjected to polyA selection for RNA library construction. The RNA-seq libraries were sequenced by the BGI Genomics using DNBseq platform (pair-end 100 bp analysis). cDNA RNA-seq of polysomal and total RNA samples