GSM7843013: CVST-2d-2; Mus musculus; RNA-Seq
At the set time points, fresh tissue samples from the bilateral cerebral cortex of mice were collected and rapidly frozen in liquid nitrogen, and then stored in a -80 ℃ refrigerator. After the tissues were homogenized, total RNA was extracted employing Trizol reagent (Invitrogen, CA, USA). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Total RNA samples were converted into cDNA libraries using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (Catalog E7530L, NEB, USA) following the manufacturer's recommendations