GSM6556587: ChIPRx_2wk_ctx_nsd1_ctrl_H3K36me3_biorep5; Mus musculus; OTHER
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using the S220 Ultrasonicator (Covaris). Sonicated S2R+ chromatin was subsequently added at 5% of total chromatin. ChIP-Rx libraries were prepared using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) and complemented with the Accel-NGS 2S Set A adapters. Up to 4ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 12-16 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent DNA D5000 Screentape Assay Kit.