GSM7805122: Parent, replicate 1, HBO1, ChIP; Homo sapiens; ChIP-Seq
Chromatin was prepared using the Cell Signaling SimpleChIP Enzymatic Chromatin IP Kit following the manufacturer's protocol (Catalog #9003) ChIPseq libraries were prepared using IDT xGen DNA library prep reagents on a Beckman Coulter Biomek i7 liquid handling platform from approximately 1ng of DNA with 14cycles of PCRamplificationaccording to manufacturer's protocol. Finished sequencing libraries were quantified by Qubit fluorometer and Agilent TapeStation 2200. Library pooling and indexing was evaluated with shallow sequencing on an Illumina MiSeq. Subsequently, libraries were sequenced on an Illumina NovaSeq6000 targeting 40 million 150bp read pairs by the Molecular Biology Core facilities at Dana-Farber Cancer Institute.