GSM7843077: shNC2; Homo sapiens; RNA-Seq
RNA was extracted using Trizol method. Total RNA with nuclease free water to final volume of 50µl is used for construction of sequencing library. Using the fragmented RNA as a template, a random primer was used for reverse transcription to achieve the synthesis of the first strand of cDNA, and the addition of 2nd Strand Marking Bu er and 2nd StrandAfter end repair, addition of base A, sequencing connector, magnetic bead screening and recovery of the target fragment, PCR amplification were carried out to complete the entire library preparation work. Enzyme Mix to synthesize the second strand of cDNA.After the library construction is completed, preliminary quantification is performed using Qubit3.0, the library is diluted to 1ng/ul, and then Agilent is used 2000.The raw off machine sequences (Raw Reads) obtained from Illumina platform sequencing are processed through processes such as removing low-quality sequences and connector contamination to obtain high-quality sequences (Clean Reads). All subsequent analyses are based on Clean Reads