GSM7663154: siCTCF rep1; Homo sapiens; Hi-C
One million siRNA-treated cells and four million of drug-treated K562 cells were individually fixed and subsequently mixed with fixed mouse 3T3 cells at a ratio of 4:1. The mixed cells were then used for Hi-C experiments using Arima-HiC kit (Arima Genomics) according to the manufacturer's protocol. Libraries were prepared using the KAPA Hyper-Prep Kit (KAPA), according to the Arima-HiC kit protocol.