GSM6625181: Tail, 1 week infection, Imatinib, rep4; Mus musculus; RNA-Seq
3-5mm sections of tail were cut fresh from the tail from areas containing granulomatous lesions and immediately placed in Trizol (Invitrogen). Tissue was cut with sterile scissors and then homogenized in the Bullet Blender Tissue homogenizer (Next Advance, Troy, NY). RNA extraction was performed in accordance with manufacture instructions. RNA libraries for RNA-seq were prepared using Clontech SmartER Stranded Total RNA-seq Kit- Pico Input Mammalian + rRNA depletion following manufacturer's protocols.