Single cell RNA sequencing of High Five cells: infected (8 hours post infection)
Around 6000 single cells were isolated using the BD Rhapsody Express Single-Cell Analysis System (BD). Sequencing libraries were prepared using the mRNA Whole Transcriptome Analysis (WTA) Preparation Protocol (BD) with unique sample tags. Sequencing was conducted on an Illumina NovaSeq 6000 spiked with 20 % PhiX, targeting 40,000 reads per cell. Files contain adapter removed and demultiplexed raw reads.