GSM8408143: SI Apc Rep3; Mus musculus; RNA-Seq
RNA was isolated using the Rneasy Micro Kit (Qiagen) according to the manufacturer's protocol. Libraries for cluster generation and DNA sequencing were prepared using an Illumina TruSeq RNA sample kit, then run on an Illumina NextSeq 500 (either 2x36 cycles, paired end (Apc, Apc Kras/+, Apc Kras/+ Pik3ca/+, Apc Kras/+ Pten, Apc Pik3ca/+, Apc Pten)  or 2x74bp paired end (Apc Pik3ca, Apc Kras/+ Pik3ca, Apc Pik3ca Slc7a5, Apc Kras/+ Pik3ca Slc7a5)).