GSM6689681: Control, IL6-, donor 3; Homo sapiens; RNA-Seq
CD8+ CAR-T cell populaiton was isolated by FACSAria III (BD Biosciences). Total RNA was isolated from the purified CD8+ T cells using the Nucleospin RNA Kit (Macherey-Nagel) according to the manufacturer's instructions. RNA libraries were prepared for sequencing using the TruSeq Standard mRNA Library Prep kit according to standard Illumina protocols