GSM7662635: hrde-1(tm1200), replicate 2; Caenorhabditis elegans; RNA-Seq
Worms were resuspended in 1 ml Trizol reagent and freeze-thawed on dry ice followed by vortexing. RNA isolation was performed by chloroform extraction and isopropanol precipitation. RNA samples were normalized to 7.5μg per 10μL, followed by polyA selection using the Dynabead mRNA Purification Kit. 100ng of polyA selected mRNA samples were treated using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manual, using NEBNext multiplex oligos for Illumina.