GSM8181401: Rest_D440_Day_3_RNA; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1Ug of total RNA was used for the construction of sequencing libraries. The NEBNext® Poly(A) mRNA Magnetic Isolation Module was used to enrich Poly(A) mRNA (input for library prep) following the manufacturer's protocol. RNA libraries were prepared for sequencing utilizing the NEBNext® Ultra™ II Directional RNA Library Prep kit (E7765L) according to the manufacturer's instructions.