GSM7898046: G-146; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol LS reagent (ThermoFisher Scientific, 10296010) in accordance with the TRIzol LS Reagent manufacturer's protocol. The total RNA was DNase treated and quantified using the Qubit RNA HS assay kit (Thermo Fisher Scientific, Q32855). RNA quality was determined on an Agilent TapeStation 4150 using an Agilent RNA ScreenTape (Agilent, 5067-5576). Total RNA libraries were generated using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/rat) (NEB #E7400) and NEBNext UltraII Direction RNA Library Prep Kit for Illumina (NEB #7760) using 10 ng of each total RNA sample.