GSM6689600: K562 Essential DHS Tiling Cas9 Biological Replicate 2; Homo sapiens; OTHER
Genomic DNA was harvested using QIAgen DNA Blood & Tissue Maxi Kit; Plasmid DNA was extracted from bacterial cultures using the QIAgen Maxi Prep Kit The sgRNA sequence present on the plasmid or integrated into genomic DNA was amplified and barcoded using PCR, from either 320 ug of genomic DNA per sample, or 5 ng of plasmid DNA