GSM7805057: Lncap cell line-Enza_2-ATAC-Seq; Homo sapiens; ATAC-seq
50,000 viable cells were precipitated and kept on ice and subsequently resuspended ATAC Resuspension Buffer (RSB) (49.25mL nuclease-free water, 500uL 1M PH7.5 Tris-HCL, 100uL 5M NaCl, and 150uL 1M MgCl2). 50uL of Transposition Master mix (25uL 2xTD buffer, 1uL Tagment DNA enzyme, 16.5uL of PBS, 0.5uL 1% Digitonin, 0.5uL 10% Tween-20 and 6.5uL Nuclease-free water) for 30min reaction at 37C with 1000 rpm mixing. DNA was then purified using Zymo DNA clean and Concentrator-5 kit. Libraries were prepared following the Buenrostro protocol (Buenrostro, Wu et al. 2015).