GSM8223863: U251 treated with DMSO, biological replicate 3; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy mini kit from QIAGEN. DNA digestion was performed on column using DNase. 200 ng of RNA was used as input for mRNA capture with oligo-dT coated magnetic beads. The mRNA was fragmented, followed by random-primed cDNA synthesis. The resulting double-stranded cDNA was used as the input to a standard Illumina library prep with end-repair, adapter ligation and PCR amplification to generate a sequencing ready library.