GSM7928366: MU2AF1-WT, scRNA-seq; Homo sapiens; RNA-Seq
live GFP+ CD34+ cells were sorted by flow cytometry and submitted for sequening For single cell RNA 3' sequencing, Sorted transduced CD34+ cell concentration and viability were evaluated using a Countess II FL Automated Cell Counter (ThermoFisher Scientific). Samples were normalized for input onto the Chromium Single Cell A Chip Kit (catalog number #PN120236, 10x Genomics), and single cells were lysed and barcoded for reverse transcription, as previously described.9 Briefly, libraries were sequenced using the NovaSeq6000 SP 100-cycle flow cell (Illumina). Sequencing analysis was performed using the 10x Genomics'