GSM7978799: uterosacral ligament, POP, rep2; Homo sapiens; RNA-Seq
Uterosacral ligament were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. Single cell suspension was loaded onto a microfluidic chip and single cell RNA-seq libraries were constructed according to the manufacturer's instructions (Singleron GEXSCOPE Single Cell RNAseq Library Kit, Singleron Biotechnologies).