GSM7290591: WT 1030 1; Mus musculus; ChIP-Seq
Fresh tissue was fixed with 1% formaldehyde for 15 minutes. After quenching and cell lysis, chromatin was sonicated using a Sonic Dismembtrator 500 (Fisher Scientific). DNA fragments with 200-500 bps were selected ChIP-seq libraries were prepared using the BIOO Scientific ChIPseq DNA library kit. 2-22 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using a thermocycler. Libraries were validated using ???