RNA-seq of amplified IP RNA from GFP-tagged ribosomes: mus musculus: adult hippocampus (pool of 4) WT_IRES-Cre replicate 5
Hippocampal RNA was isolated from immunoprecipitated GFP-tagged ribosomes. Four RNA samples were pooled (100pg total) and amplified before library preparation. Libraries were prepared with the Illumina TruSeq Stranded mRNA kit and run on NextSeq sequencing SE-75 by the UTSW Genomics Sequencing Core.(sex, side injected: male, right; female, left; male, left; male, left)