GSM7660157: Cfpac1 cells, ChIPseq H3K27ac, Trametinib 3h, Rep2; Homo sapiens; ChIP-Seq
ChIP-seq was carried out as previously described (PMID 32997974) using 1x106 cells for the H3K27ac and H3K9me3 ChIP and 20×106 cells for the ELF3 ChIP. The following antibodies were used:  anti-H3K27ac (Abcam, ab4729), anti-H3K9me3 (Abcam, ab8898) and anti-ELF3 antibody (rabbit polyclonal antibody generated in-house using a peptide corresponding to aa1-150 of the human ELF3 reference protein sequence). DNA libraries were prepared as described (PMID 29706544) and sequenced on Illumina NextSeq 500 and NovaSeq6000 platforms. All ChIPseq data sets were processed in single-end.