GSM7842822: M2-ATAC; Homo sapiens; ATAC-seq
We performed ATAC-seq experiments with some modifications based on the protocol described by Daniel [16, 17]. M0, M1, and M2 macrophages were isolated and the number of cells was adjusted to 50 cells/ml in phosphate buffer saline (PBS). The nuclei of these macrophages were then isolated using ATAC-LB. Following DNA fragmentation and purification, the DNA was amplified through 14 cycles. To assess the fragment distribution of the libraries, an Agilent Bioanalyzer was used, and the libraries were subsequently sequenced on a HiSeq 2500 platform.