GSM7661291: Kidney, Foxp1KspKO mice, Input; Mus musculus; ChIP-Seq
cells were lysed and the chromatin was sheared to a size range of 150 to 300 bp using the sonicator (Diagenode, Bioruptor Pico). For each IP to be performed, aliquot 30μL magnetic Protein G beads (Invitrogen; 1003D) were used to preclear the chromatin, and incubated at 4℃ overnight with the previously characterized Foxp1 polyclonal antibody or control rabbit IgG (2729S; Cell Signaling). Immune complexes were handled according to the manufacturer's protocol. Reverse cross-linked immunoprecipitated chromatin was extracted using QIAquick PCR Purification Kit. The library was quantified using the QubitTM dsDNA HS Assay Kit (Invitrogen; Q32851) by QubitTM Fluorometer, inspected with Agilent bioanalyzer and StepOnePlus Real-Time PCR.