GSM7928135: TumorOrg.P21.Rep2; Homo sapiens; RNA-Seq
Organoids were flash frozen by liquid nitrogen, and RNA was extracted using QIAGEN Rneasy mini kit. The cDNAs were synthesized from total RNA using random primers. The ribose diols in the 5' cap structures of RNAs were oxidized, and then biotinylated. The biotinylated RNA/cDNAs were selected by streptavidin beads (cap-trapping). After RNA digestion by RNaseONE/H and adaptor ligation to both ends of cDNA, double-stranded cDNA libraries (CAGE libraries) were constructed.