GSM7843135: Vehicle 3 day 3; Rattus norvegicus; RNA-Seq
Two months old male Lewis rats were treated daily with subcutaneous injection of spironolactone for three days. Rats were then euthanized, eyes were removed and the peripheral cornea and limboscleral tissues dissected for RNA sequencing. Total RNA was isolated from tissues using the RNeasy Mini Kit inluding DNase I treatment (Qiagen, Courtaboeuf, France) RNA libraries were prepared for sequencing using standard Illumina protocols