GSM6901164: Nutritive Flow Biol Rep 1; Homo sapiens; RNA-Seq
Cells were lysed with 1% beta mercaptoethanol in Buffer RLT lysis buffer (Qiagen) and RNA was isolated using the Rneasy Micro kit (Qiagen). RNA with RINe above 8.0 were submitted for sequencing at the Genome Technology Access Center at Washington University in St. Louis. Paired-end libraries were prepared using Clontech SMARTer Illumina Kit and sequenced on a NovoSeq 6000 (Illumina).