GSM7979120: primary cultured mouse hepatocyte, H3K4me3,  ChIP; Mus musculus; ChIP-Seq
Cells were fiwed with 1% formaldehyde for 10 minutes. After quenching and cells lysis,chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the KAPA HTTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and IP samples. Libraried were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp lenght using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.