GSM7091017: Mouse Liver tumor, Nrf2 KO, AcH3K27, rep2; Mus musculus; ChIP-Seq
Liver tumor tissues were crosslinked with 1% formaldehyde, sonicated, and immunoprecipitated with AcH3K27 antibody. 10 ng ChIP pulldown DNA was prepared for the library using TruSeq ChIP Sample Preparation kit (Illumina). Libraries were amplified using 18 cycles on the thermocycler, followed by size selection at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter.