GSM6625320: Isogenic 16p11.2 duplication, 7WIV iEN, clone 1 , Sample A; Homo sapiens; RNA-Seq
Total RNA extraction was performed as per manufacturer's instructions using the mirVANA miRNA isolation kit (Thermofisher, #AM1560) The Illumina TruSeq Stranded Total RNA Library Preparation Kit was used to prepare sequencing libraries from 100 ng of total RNA samples RNA-SEQ (lllumina HiSeq 4000 Sequencer was used to sequence the libraries with the production of single-end, 50 bp reads at the sequencing depth of at least 20-25 million reads per sample).