GSM8408115: wt-1; Sorghum bicolor; RNA-Seq
Total RNA was isolated from fully developed leaves of wild-type and transgenic sorghum plants using an RNeasy Plant Mini Kit according to the manufacturer's protocol (Qiagen, Germantown, MD). RNA was cleaned from DNA contamination using TURBO DNA-free™ Kit (ThermoScientific, Waltham, MA). To determine expression level of transgenes, first-strand cDNA was synthesized from 1 µg of total RNA with a High-Capacity cDNA Reverse Transcription Kit (ThermoScientific) DNBSEQ Eukaryotic Strand-specific mRNA library prepared by BGI Americas.