GSM6448488: HL60_3uM_A485_R2; Homo sapiens; RNA-Seq
Total RNA was isolated with Qiagen Rneasy Mini Kit. SIRV-Set 3 RNA was spiked into samples. 4SU-labeled transcripts were alkylated by addition of 5 µL 100 mM iodoacetamide, 25 µL DMSO, and 5 µL of sodium phosphate buffer (0.5M, pH 8) and incubating at 50C for 15 minutes. 3' mRNA libraries were prepared with Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Cat. No. 015) according to manufacturer's instructions.