GSM6504891: mir-125b-2 P0 WT; Mus musculus; RNA-Seq
Mice hippocampus was isolated and digested by QIAzol, and then extracted by Qiagen miRNeasy Micro kit (# 217084). Six WT or six maternal KO total RNA samples were pooled together equally as one to get pooled WT and pooled maternal KO sample. Before libray, perform rRNA depletion with Ribo-Zero Gold rRNA Removal Kit(#MRZG126). Then, Library were preparaed according to the Illumina's official protocol by Agilent's SureSelect Strand-Specific RNA Library Preparation Kit(#G9691A).