GSM7714881: Candida glabrata, treatment,rep3; Nakaseomyces glabratus; RNA-Seq
RNA was extracted using Invitrogen TRIzol® Reagent. 1 ug of total RNA was used for the construction of sequencing libraries. The fragmented mRNA was reverse transcripted with SuperScript II and then converted to double-stranded cDNA using RNaseH and DNA Pol I by random priming. After purification, the double-stranded cDNA was subjected to blunt-ending, dA addition to 3'-end and adapter ligation. Finally, PCR was carried out to enrich the adapter ligated cDNA and the libraries were analyzed by Agilent Bioanalyzer 2100 and quantified by qPCR before sequenced by the Illumina sequencing platform.