GSM7898673: ARLA01and05, VDJ; Homo sapiens; RNA-Seq
Cd4+ SF T cells were einriched from SF mononuclear cells either by FAS sort (ARLA01 and 05) or enriched in CD4+ T cells using the EasySep™ Human CD4+ T Cell Isolation Kit (stemcell technologies, Vancouver, Canada, ARLA02). The library for 5 ' scRNA and VDJ TCR sequencing was prepared following 10X genomics instructions. For ARLA01 and 05 a hashtag library was included: TotalSeqC Hashtag Oligos (Biolegend; ARLA01: GTCAACTCTTTAGCG, ARLA05: TTCCGCCTCTCTTTG).