GSM7927988: pbmc,18c_TCR; Homo sapiens; OTHER
Thawed PBMC were rested for 1hour in RPMI 1640 supplemented medium. Different time points per donor were labeled with different TotalSeq anti-human hashtag antibodies. Single-cell transcriptome sequencing and scTCR-seq were performed on FACS sorted gamma-delta T cells and B cells using the Chromium Single-Cell 5′ Library Gel Bead and Construction Kit and the Chromium Single-Cell V(D)J Enrichment Kit according to the user guidelines. scRNA-seq, scTCR-seq, Cell surface protein library.