GSM6628954: A18w_Mcph1KO_RRBS; Mus musculus; Bisulfite-Seq
Every tissue in our study were chopped into small pieces, and half of homogenate from one tissues were used to extract the genomic DNA using the DNeasy Blood & Tissue Kits (Qiagen), and half of homogenate from one tissues were used to extract the total RNA using the Total RNA Extraction Kit (Omega). Genomic DNA (100 ng) was used to construct the library of RRBS and oxRRBS [46]. RRBS and oxRRBS libraries were prepared by Ovation® RRBS Methyl-Seq with the TrueMethyl® oxBS kit (Tecan, Redwood City, CA, USA) according to the manufacturer's instructions.