GSM8181704: 26591_R1_FP_37exp; Saccharomyces cerevisiae; OTHER
Cells were mixermilled with frozen buffer (20mM Tris pH8, 140mM KCl, 1.5mM MgCl2, 100ug/ml cycloheximide, 1% Trition X-100), buffer was thawed, spun at 3Kg  for 5 minutes, then supernatatant was spun at 20Kg for 10 minutes. As described in Powers and Brar, 2021, using linker ligation, circularization size selection of 28-31 nt for ribosome footprints, 50nt for mRNA, dephosphorylation, linker ligation, RT, circularization, PCR