GSM6505954: Spleen, Cdca7G305V/G305V, ATAC; Mus musculus; ATAC-seq
In brief, single cell suspensions were prepared by squeezing the tissues through a 35-μm nylon cell strainer (352235, Corning). 50,000 mouse splenic cells were resuspended in 50 µL transposition mix (Nextera) and incubated for 30 min at 37 °C ATAC-seq libraries were prepared as previously described (Buenrostro et al, 2013). Libraries were amplified by PCR with barcoded Nextera primers.