GSM7927928: WT treated; Mus musculus; RNA-Seq
WT and Irf4−/− mice were intravenously injected with 2.5 × 105 B16-F10 cells. Twenty days post-injection, splenic NK cells were isolated using the EasySep Mouse NK Cell Isolation Kit, followed by further purification through flow-sorting the CD3−NK1.1+ cell population. Total RNA was extracted from the sorted NK cells using the RNeasy Plus Mini Kit. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.