Whole genome sequence of Cytobacillus firmus A21
Genome sequencing involves isolation and purification of genomic DNA, fragmentation, ligation to sequencing adapters and purification. Following the amplification and denaturation steps, libraries was pooled and sequenced. 50 ng of DNA was used to prepare the libraries using Nextera DNA Sample Preparation Kit (Illumina). Library insert size was determined by Experion Automated Electrophoresis Station (Bio-Rad). The insert size of the libraries ranged from 300 to 850 bp (average 500bp). Pooled library (12pM) was loaded to a 600 Cycles v3 Reagent cartridge (Illumina) and the sequencing was performed on Miseq (illumina)