ChIRP-seq of Plasmodium falciparum targeting Lnc-ch14, ring stage (negative control)
Libraries from the ChIRP samples (RNA pulled down by specific probes, converted to cDNA) were prepared using the KAPA Library Preparation Kit (KAPA Biosystems). Libraries were amplified for a total of 12 PCR cycles (12 cycles of [15 s at 98C, 30 s at 55C, 30 s at 62C]) using the KAPA HiFi HotStart Ready Mix (KAPA Biosystems).