16S metabarcoding of microbial mat: ECB10A
DNA was extracted from microbial mat samples. We targeted a 312 bp region of the rbcL plastid gene for metabarcoding, and amplified this using specific primers with PCR. A second PCR clean-up was performed, and samples were quantified using a Qubit Fluorometer. Libraries were loaded with 25% PhiX clustering control on the Illumina MiSeq platform for 300 bp  2 paired end reads using the V3 kit.