GSM8415040: Liver_HR_15M5T_4me3_2021; Mus musculus; ChIP-Seq
Chromatin was cross-linked with formaldehyde, the reaction stopped with glycine, and sonicated with a bioruptor to obtain fragment sizes of 100–300 bp for ChIP-seq. ChIP-validated antibodies used were directed against H3K4me3 (Active Motif #39915), H3K4me1 (Abcam ab8895), H3K27ac (Abcam ab4729), H3K27me3 (Active Motif #61017), and H3K9me3 (Active Motif #61013). Immunoprecipitated DNA was recovered and purified using the QIAquick PCR Purification kit (Qiagen) QIAseq FX DNA library kit or the QIAseq Ultralow Input Library Kit