GSM8223757: Eu_root_rna_rep2; Elaeagnus umbellata; RNA-Seq
Fresh roots, and nodules if available were gathered from Coriaria nepalensis, Elaeagnus umbellata, Alnus trabeculosa,  Chamaecrista pumila, and Mimosa pudica subsequently froeezed in liquid nitrogen. RNA was extracted using the Trizol (Invitrogen, USA).  For mycorrhizal inoculation of Populus deltoides × P. euramericana cv. ''Nanlin895'', three cm terminal cuttings were taken from 4-week-old Populus plants and rooted for two weeks in Lloyd & McCown's woody plant medium supplemented with 20 g L-1 sucrose and 5 µM indole-3-butyric acid (Sigma Aldrich). Rooted plants were transplanted to a mixture of vermiculite, sand, and perlite (3 : 1 : 1) containing Rhizophagus irregularis spores and cultured in phytotron with 12 h photoperiod at 25 °C constant temperature. The spores were produced by co-culturing R. irregularis with leeks and diluted to a final concentration of 400 spores per plant 11. The roots of AM-colonized and non-colonized Populus plants were harvested after 8 week post-inoculation and subsequently powdered in liquid nitrogen. Total RNA was extracted using the TRIzol reagent (Invitrogen, USA), three biological replicates were used for each sample. For roots and nodules samples, rRNAs were first depleted from total RNA using Ribo-off rRNA Depletion Kit (Vazyme, Cat. No.: N409-02). Stranded library was then prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, Cat. No.: NR604-01) and sequenced using Illumina NovaSeq 6000 by Frasergen Bioinformatics Co., Ltd. (Wuhan, China).  For mycorrhizal inoculation of Populus deltoides × P. euramericana cv. ''Nanlin895'', unstranded libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions and sequenced with Illumina NovaSeq 6000 by OE Biotech Co., Ltd. (Shanghai, China). ﻿