GSM8223198: GEX1_Batch1, mRNA- and ADT-derived cDNA; Homo sapiens; RNA-Seq
Single-cell library preparation for surface protein, mRNA,  and TCRs was performed using the Chromium Single Cell  V(D)J Reagent Kit v1.1 (10x Genomics). The TCR V(D)J  region was specifically enriched in a separate library preparation  using the Chromium Single-Cell V(D)J Enrichment Kit  (10x Genomics). Cell suspensions were combined with barcoded,  single-cell 5′ gel beads and loaded onto Chromium Next GEM  Chip G (10x Genomics) at a limiting dilution such that a single  bead and a single cell are partitioned into a sphere. Libraries were  sequenced by Illumina sequencing (NextSeq 500/550 platform). GEX: Single Cell 5' R2-only. TCR: Single Cell V(D)J R2-only.