GSM6593401: Pig1-1_input; Homo sapiens; OTHER
RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis. RNA was randomly fragmented to approximately 200 nt through RNA Fragmentation Reagents and protein A/G beads were connected to an m1A antibody (202003, Synaptic Systems, Germany) by rotating at RT for 1 h. The RNA fragments were incubated with the beads at 4 °C for 4 h. Then, the captured RNA was eluted from the beads and extracted with TRIzol Reagent (Invitrogen, USA). Both the input sample and the m1A IP sample were subjected to library generation with the NEBNext Ultra RNA Library Prep Kit (New England Biolab, UK).