GSM7290803: Plasma_treat4h_1; Mus musculus; RNA-Seq
Total RNA was extracted from B16 melanoma cells. The integrity of total RNA was analyzed using the 2100 Bioanalyzer instrument of Agilent Technologies. The RNA integrity number (RIN) values for all samples were > 8. Poly(A) mRNA isolation from total RNA and fragmentation was conducted using the Illumina TruSeq Stranded mRNA Prep Kit (Illumina, San Diego, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols