Single nucleus RNA sequencing data from human heart biopsies
Single nucleus RNA sequencing data from human heart biopsies
Two pieces of tissue were obtained from patients that went through endomyocardial biopsy as part of the clinical routine practice of diagnostic or follow up procedure. For enzymatic dissociation hearts were minced into small pieces and were placed into a six-well dish filled with RPMI 1640 medium containing 2% FCS, 20 mM HEPES (Lonza), 1 mg/ml collagenase D (Sigma), and 25 μg/ml DNase I (Applichem) and incubated at 37°C under continuous stirring. The remaining tissue pieces were mechanically disrupted.  Nuclei were isolated from frozen patient derived EMBs and sorted to enrich intact nuclei using a BDFACSMelody. Sorted single nuclei suspensions were emulsified for library generation using the droplet-based 10x Chromium (10x Genomics) system. cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v3 Chemistry).