GSM8004101: VEGF_r2; Mus musculus; RNA-Seq
RNA isolation was performed on frozen cell pellets on DNA/RNA Shield (Zymo research) using Direct-zol RNA Miniprep kit (Zymo Research). RNA concentration was measured by NanoDrop 1000 (Thermo Fisher Scientific) and RNA integrity was determined using Bioanalyzer (Agilent) Library construction of 300 ng total RNA for each sample was made using KAPA Stranded mRNA-Seq Kit (Illumina Platforms; Kapa Biosystems) using 10 cycles of PCR amplification. Libraries were purified using AxyPrep Mag PCR Clean-up kit (AxygenTM). Each library was quantified using a Qubit fluorometer (Life Technologies) and the size distribution assessed using the 2,100 Bioanalyzer (Agilent Technologies, Santa Clara, USA).