GSM6625051: Gut-STAT3ko-PD-1-ko-to-WT-CD4-2-α; Mus musculus; RNA-Seq
RNA from equal numbers of sorted H-2Kb+TCRβ+CD4+ and H-2Kb+TCRβ+CD8+ cells were extracted with the RNeasy Mini Kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. TCR-CDR3 libraries were prepared using Takara Smarter Mouse TCR a/b Profiling kit following manufacturer's recommendations