GSM8181193: ventricles, lincRNA-p21 knockout mice, TAC surgery, replicate 2; Mus musculus; RNA-Seq
5ug total RNA was extracted from left ventricle apex of lincRNA-p21 global knockout mice and littermates, 8 weeks after sham or TAC surgery, using TRIzol Reagent (Thermo Fisher Scientific, #15596026). The enriched ployA RNA was used to prepare 12 barcoded Illumina libraries with TruSeq Stranded mRNA Library Prep Kit, following the manufacturer's instructions (Illumina).