GSM7715141: HPC-1 3wk rep #3; Mus musculus; RNA-Seq
Spine, femurs, tibia, and iliac bones were crushed in PBS+2% FBS. Bone marrow was enriched for cKit, followed by FACS sort of live LSK SLAM (CD3e-, B220-, GR-1-, CD11b-, TER119-, cKIT+, SCA-1+, CD48-, CD150+) HSCs into sterile PBS+RNAse inhibitor and snap freezed at -80 degrees. cDNA syntesis and amplification were done using SMART-Seq v4 (Takara), followed by library preparation using Nextera XT DNA library prep kit (Illumina).