GSM8414927: heart,MI3d,WT; Mus musculus; RNA-Seq
Left ventricular (LV) tissues were isolated 3 days after MI, minced and washed in ice-cold RPMI1640 and digested using Multi-tissue dissociation kit 2 (Miltenyi, 130-110-203) according to the manufacturer's protocol. Digested cells were filtered and centrifuged to obtain a single-cell suspension. Cell debris  were removed using Debris Removal Solution (Miltenyi Biotec,130-109-398). Cells were washed in the RPMI1640 and then resuspended at 1×106 cells/ml in 1×PBS and 0.04%BSA. Cells were loaded into Chromium microfluidic chips and barcoded within a 10x Chromium Controller (10x Genomics). The single-cell transcriptome was reverse-transcribed into a cDNA library which contain 10X cell barcodes and unique molecular identifiers constructed with reagents from the Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1 (10x Genomics,1000165) according to the manufacturer's protocol.