RNA sequencing of MOLM-13 and KG-1a cell lines after inhibition of S100A9 and BCL2
RNA sequencing of MOLM-13 and KG-1a cell lines after inhibition of S100A9 and BCL2
AML cell lines were pre-treated with siRNA against S100A9 for 24 hours and were afterwards incubated with ABT-199 for an additional 24 hours. Total RNA was extracted and purified using the NucleoSpin RNA plus kit (Macherey–Nagel, Düren, Germany). Sample quality was checked by calculating the RNA integrity number (RIN value) using fragment analyser (Agilent), with 3 biological replicates from each group. The RNA sequencing (RNA-seq) library preparation was performed with 150 ng RNA using the Illumina KAPA RNA HyperPrep kit with RiboErase (HMR) (Illumina, Cambridge, UK).