GSM7663271: EndoC-βH1 cells, DMSO control, rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted in TRIzol (Invitrogen) and DNase I treated using the Directzol RNA Miniprep kit (Zymo Research) RNAseq libraries of polyadenylated RNA were prepared using the TruSeq RNA Library Prep Kit v2 (Illumina) or TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions. cDNA libraries were sequenced with pair-end 51 bps using an Illumina NovaSeq6000 platform.