GSM8136546: UNG AxD; Homo sapiens; RNA-Seq
Single-cell suspension was prepared from organoids using combination of mechanical and enzymatic dissociation. The organoids were chopped to small pieces, treated with papain, and repeatedly resuspended by pipetting and subsequently incubated on an orbital shaker. The single-cell suspension was then fixed using 10x Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling, 10x Genomics protocol and kit, and the sequencing libraries were prepared using Chromium Fixed RNA Kit. Chromium Fixed RNA Kit (10x Genomics, Pleasanton, CA)