GSM8003763: 2_G1, choroid plexus; Mus musculus; RNA-Seq
Mouse brain choroid plexus tissue samples were dissected under the microscope immediately after CO2 euthanasia. These tissues were snap-frozen on dry ice. Nuclei from the choroid plexus tissues from ApoE-KO and ApoE-KO/APOL1-G1 mice were prepared as follows. Snap-frozen choroid plexus tissues were lysed in EZlysis buffer (#NUC101-1KT, Sigma-Aldrich, St. Louis, MO) and homogenized 15 times using a loose Dounce homogenizer and 5 times in a tight pestle. After 5 min incubation, the homogenate was passed through a 40 µm filter (43-50040, PluriSelect, El Cajon, CA) and centrifuged at 500 g at 4°C for 5 min. The pellet was washed with EZlysis buffer and again centrifuged at 500g at 4°C for 5 min. The pellet was resuspended with Dulbecco's phosphate-buffered saline (DPBS) with 1% FBS to make a final nuclear preparation for loading on to a 10x Chromium Chip G (10x Genomics, Pleasanton, CA) formation of gel beads in emulsion (GEM). 10x Genomics Chromium 3' V3.1 mRNA-seq library protocol