GSM7184522: M12_CD73neg; Mus musculus; RNA-Seq
Mouse retinas were dissected and dissociated as previously described (Fadl, et al. Mol Vis 2020). One sample from each timepoint was used to deplete CD73 positive cells (rod photoreceptors) using Anti-CD73 conjugated magnetic beads. Whole retina and CD73 negative dissociate was used as input for 10X Genomics Chromium controller using v3.1 (Chromium Next GEM Single Cell 3' GEM, Library and Gel Bead Kit v3.1) chemistry. Approximately 17,000 live cells per sample were loaded into the Chromium chip following the Cell Suspension Volume Calculator Table to capture transcripts from approximately 10,000 cells. Libraries were performed according to the manufacturer's instructions (Single Cell 3' v3.1 protocol, 10x Genomics).