GSM8137079: SD2 response evaluation; Homo sapiens; RNA-Seq
Total RNA was extracted with the RNeasy Mini Kit (Qiagen, Germany). Library construction commenced with 1–3 ng of RNA input. AmpliSeq cDNA Synthesis for Illumina (Illumina, San Diego, CA, USA) was utilized for cDNA synthesis. For RNA library preparation, the AmpliSeq for Illumina custom RNA panel (Illumina), AmpliSeq library PLUS for Illumina (Illumina), and AmpliSeq CD indexes Set A for Illumina (Illumina) were employed, with the custom panel comprising 373 genes associated with cancer progression.