GSM7663166: DKO rep1; Homo sapiens; ATAC-seq
ATAC-seq was performed using the Diagenode ATAC-seq Kit (C01080002, Diagenode) following the manufacturer's protocol. Briefly, 200,000 EV, S1KO, S2KO and DKO K562 cells or 50,000 drug-treated cells per sample were harvested and nuclei were isolated. Immediately following the nuclei prep, the pellet was resuspended in 50 µl tagmentation mix. The transposition reaction was carried out for 30 min at 37C. The tagmented DNA was then purified and eluted in 12 µl of DNA Elution Buffer. Library was constructed using 10 µl of tagmented DNA and 1 µl of primer pair provided by the 24 UDI for Tagmented libraries Set I (C01011034, Diagenode)  following the manufacturer's protocol. Libraries were amplified using 11-16 cycles on the thermocycler. The DNA fragments with target sizes ranging from 200 bp to 1000 bp were selected using AMPure XP beads (Beckman Coulter) and validated using the Agilent High Sensitivity DNA kit.