RNA-seq of Pinus pinaster: embryogenic lines matured at 28 C
Sequencing libraries were constructed by using 0.5-1.0 g RNA per sample. Integrity of total RNA was assessed with the Fragment Analyzer (Agilent) using the DNF-471 RNA Kit (15nt). Sequencing libraries were generated using TruSeq Stranded mRNA Library Prep Kit (Illumina) by following manufacturers recommendations with PolyA selection for ribo depletion