GSM6450184: Ckit negative, scRNAseq; Mus musculus; RNA-Seq
2500 mouse bone marrow E-cadherin+;cKIT+, Ecadherin+;cKIT- and LK cells were sorted and plated in duplicate in Methocult (Stemcell technologies, GF M3434). Colonies formed were characterized and counted 5-14 days after plating. E-cadherin+;cKIT+, Ecadherin+;cKIT- and LK cells were sorted and used at a 2x single cell suspension at final conc. 1000 cells/µl in 1xPBS containing 0.04% weight/volume BSA (400 µg/ml). Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics)