GSM8414608: SW1573 DMSO; Homo sapiens; ATAC-seq
100,000 SW1573 and H358 cells cells were used to isolate nuclei. Nuclei were incubated with tagmentation Master Mix at 37 °C for 30 minutes after lysing the cells in ice-cold ATAC Lysis Buffer and the DNA was purified with the DNA purification column. PCR amplification of tagmented DNA was performed to make libraries with the appropriate indexed primers.