Fungi_Grape Must_VIN_B_Faba Beans
The BITS (ACCTGCGGARGGATCA) and B58S3 primers (GAGATCCRTTGYTRAAAGTT) were used to amplify the ITS1 region of fungal communities. A total reaction mixture of 30 L 2 Ion 16S Metagenomics Environmental Master Mix (Life Technologies, United States), 30 ng DNA template, 0.25 M of each primer, and sterile dH2O was used for PCR amplification. The fungal communities were amplified following 94C of initial denaturation for 4 min, 40 cycles of 94C for 30 s, 54C for 30 s, and 72C for 45 s each, and 72C for 10 min. The presence of PCR amplicons was verified on a 1% EtBr-stained agarose gel containing 1 TAE buffer.