GSM7898123: Lung 4 scRNA seq GEXP; Rattus norvegicus; RNA-Seq
Freshly collected lungs were extracted. Half of one lobe was dissociated using the Mouse Lung Dissociation kit (Miltenyi Biotec) with the gentleMACS Octo Dissociator following the manufacurer's recommendation. Four samples were pooled and labeled using 10X Genomics CellPlex (PN-1000261) before loading onto Chip G and the Chromium Controller. Single Cell Libraries were generated using Next GEM Single Cell 3' reagents v3.1 with dual indexing (10X Genomics). Library quality was assessed with a High Sensitivity DNA Chip on a BioAnalyzer (Agilent). Sequencing was done on a NovaSeq at MedGenome (Foster City, CA), 500M Paired-End Reads per Library.