GSM7090804: cell, infection, rep3; Homo sapiens; miRNA-Seq
Total RNA was extracted using RNeasy Micro Kit (Cat# 74004, Qiagen)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent 2100 Bioanalyzer/Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US)/.Qualified total RNA was further purified by RNAClean XP Kit (Cat A63987, Beckman Coulter,Inc.Kraemer Boulevard Brea, CA,USA)and RNase-Free DNase Set  (Cat#79254, QIAGEN, GmBH, Germany). According to the experimental instructions, the purified Total RNA was subjected to 3 'end joint connection, 5' end joint connection, reverse transcription, amplification, size selection of cDNA library, purification and other steps to complete the construction of sequencing sample library.