Illumina HiSeq 2500 paired end sequencing; RNA sequencing of thirty-eight basal tumour samples from CLL patients
RNA sequencing of thirty-eight basal tumour samples from CLL patients
Thirty-eight basal tumour samples were obtained from CLL patients diagnosed using the International Workshop on Chronic Lymphocytic Leukemia (iwCLL) guidelines and managed at Southampton General Hospital (Southampton, UK). Informed consent was obtained in accordance with the declaration of Helsinki and the study was approved by our regional research ethics committee. RNA was extracted using miRNeasy kits (Qiagen) from 1x10^6 cells as per manufacturer instructions mRNA libraries were prepared using TruSeq RNA Library Prep kit (Illumina, Hayward, CA, USA)