GSM6690463: SV, 2; Mus musculus; RNA-Seq
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit including DNASE1 treatment. Quality control was performed with Agilent Bioanalyser. RNA-Seq library preps were constructed using the Illumina TruSeq® Stranded mRNA Library Prep kit (Cat #20020595) using 150ng of total RNA as input with 13 cycles of PCR amplification RNA-seq: Sequencing was paired-end 50 cycles using an SP100 cycle flowcell-v1.5 on an Illumina NovaSeq6000 sequencer with 2% PhiX spike-in.