GSM7661108: Activated, Isotype Control Antibody Biological Replicate 5; Homo sapiens; RNA-Seq
Live CD4+ T cells were sorted collected into eppendorfs. Cells were lysed in 100uL extraction buffer using Machery Nagel RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI