GSM7927730: PpardECKO, lung, LPS; Mus musculus; RNA-Seq
Mouse lung tissues were harvested and single cell suspensions were prepared for flow cytometry sorting. Viable CD45−CD326−CD144+CD31+ ECs were sorted into collection buffer (DMEM/F12 supplemented with 10% FBS) using BD Aria Fusion Cell Sorter. ScRNA-seq libraries were prepared using Chromium Single Cell 3' Reagent Kits v3.1 (10x Genomics; Pleasanton, CA, USA) according to the manufacturer's instructions.