GSM7979036: Kidney_1535_post (R9953-7_S74); Homo sapiens; RNA-Seq
8ml of blood was drawn into the ACD tube.  After Ficol density gradient centrifugation, PBMCs were separated isolated  and cryopreserved in FCS/DMSO. mRNA libraries were constructed using KAPA RNA HyperPrep Kit following the manufacture's instruction (Cat# KK8540).  RNA was sheared and primed with oligos for cDNA synthesis.  After adapter ligation and PCR amplified, the final library was quantified and accessed quality with TapeStation.