GSM8223573: RPM in vitro replicate 5 (bulk RNAseq); Mus musculus; RNA-Seq
For bulk RNA sequencing, cells were harvested by centrifugation and resuspended in Trizol. RNA was isolated with Arcturus Picopure RNA isolation kit or Direct-zol RNA Microprep. For snRNAseq, tumors were harvested and either snapfrozen or embedded in OCT for cryosectioning. For snap-frozen tumors, nuclei were isolated with manual homogenization with Dounce homogenizer. For cryo-sectioned tumors, nuclei of cryosectioned scrolls were isolated with the Singulator. For RNA-seq, library was constructed with NextSeq 500/550 High Output kit v2.5 (75 cycles) (Illumina #20024906) For snRNA-seq, library was constructed according to manufacturer's instructions (10x Genomics 3' Gene Expression) with a barcode plate Dual Index Kit TT Set A