GSM7124101: 28S WT Sample 1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using Trizol Reagent according to the manufacture's protocol. Following phase separation, the aqueous phase was applied to a RNeasy Mini Kit column and RNA was purified according to kit instructions. The 28S and mt-ND5 transcripts were reverse transcribed using transcript-specific primers downstream of the m1A sites with TGIRT following the manufacturer's protocol. Transcripts were amplified with barcoded oligos targeting either 28S or mt-ND5. Barcoded samples were pooled and sequencing on an Illuminia Micro Miseq