scRNA-seq of homo sapiens: relapsed or refractory MDS or AML (Timepoint:C2)
10x Genomics 5' scRNA-seq; Thawing of cryopreserved sample using PBS supplemented with 10% BSA and 10% DNase I, grade II and resuspension at 1,000 cells per µl in PBS + 0.04% BSA. Loading of 17,000 cells onto 10x Chromium Chip K (10x Genomics, Cat. no. 1000286) followed by library preparation of single cell cDNA using 10x Genomics 5' scRNA-seq v2 chemistry (Cat. no. 1000263) according to manufacturer's instructions.