GSM8004586: Hypoxia-enhanced kidney organoids; Homo sapiens; RNA-Seq
To obtain single cells from kidney organoids, detached organoids were harvested by gentle pipetting and then dissociated using Accutase for 20 min. Isolated cells were washed and resuspended in PBS containing 0.04% BSA. Cells were prepared according to manufacturer's protocol (10X Genomics). Sequencing libraries were constructed using 10X Chromium Next GEM Single Cell 3' reagent kit v3.1 (10X Genomics).