GSM7398496: rat_iOL_26c; Rattus norvegicus; OTHER
~5 million rat iOL were treated with vehicle or 26C and then crosslinked with 1% formaldehyde for 10 min at room temperature and lysed with 1× Nuclease Digest Buffer. Chromatin was digested by Enzyme Mix and sonicated using Covaris sonicator then processed with chromatin immunoprecipitation using the H3K27ac antibody The DNA libraries were amplified using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645, New England Biolabs) according to its recommended protocol and sequenced by NovaSeq 6000 system on pair-end 2×150 bp mode