Duplicate RNASeq of clinical isolate of C.neoformans culture 35
The libraries of the QC passed RNAs were prepared using TruSeq Stranded mRNA library prep kit from Illumina (San Diego, CA, USA) as per the kit instructions. Briefly, the extracted total RNA was subjected to fluorometric quantification using Invitrogen Qubit Fluorometer to measure the concentration of RNA accurately. The Poly-A containing mRNA molecules were purified using poly-T oligo attached magnetic beads. Following purification, the mRNA was fragmented and reverse transcribed to cDNA maintaining the strand specificity. Adapters were ligated to the double-stranded cDNA and were purified with AMPure XP beads. The products were enriched with PCR and purified to create the final cDNA library. The DNA libraries were quantified in order to optimize the cluster densities across every plane of the flow cell.  The library quality was checked using Bioanalyzer DNA 1000 chip with 1 l undiluted DNA library.