RNA-Seq of Optic Nerve Head: PT-CEI 7D
Optic nerve head (ONH) mRNA profiles collected immediately after (0hr), 1, 2, 3, 7, and 10 days following PT-CEI or normotensive CEI and naive samples. ONHs were removed, flash frozen on dry ice, and stored at -80 until ready for RNA extraction. RNA was extracted with the MiRNEasy Kit from Qiagen in accordance with manufacturer's guidelines. Total RNA was used as a template for libarary preparation using the Ovation RNA-seq System.