RNA-seq of PDX305 Ewing sarcoma cell line, rep 3
PDX305 cells were grown in RPMI 1640 media (Gibco) supplemented with 10% FBS (Atlas Biologicals) and 2mmol/L-glutamine  Cells were cultured at 37C with 5% CO2 and were split according to ATCC and COG recommendations.  RNA was extracted with Qiagen Rneasy kit. Libraries were prepared with NEBNext Ultra II RNA Library Prep kit. Sequencing was performed by Novogene.