GSM7763695: RE_H3K9ac_rep1_IP; Oryza sativa; ChIP-Seq
Rice seedling strips were crosslinked with 1% formaldehyde (SIGMA, F8775) for 10–15 min at room temperature in a vacuum chamber, and 0.2 M glycine was applied to quench the reaction. For each reaction, ~0.1 g sample was ground in liquid nitrogen into fine powder and then lysed in 300 μL of Buffer S (50 mM HEPES-KOH (pH7.5), 150 mM NaCl, 1 mM Ethylene Diamine Tetraacetic Acid (EDTA), 1% Triton X-100, 0.1% sodium deoxycholate, 1% SDS) for 10 min at 4°C. The homogenate was mixed with 1.2 mL of Buffer F (50 mM HEPES-KOH (pH 7.5), 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate). After that, the chromatin in the homogenate was fragmented to 200–