GSM6560247: 55N, H3K27me3, ABCAM, control1; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes and quenched with 0.125 M glycine. After cell lysis, chromatin was sonicated using a Diagenode Bioruptor (30 seconds on and 30 seconds off) for 10 minutes in precooled conditions. Lysates were pelleted at 4˚