GSM6509436: E13_Midbrain_Ctrl_rep3; Mus musculus; RNA-Seq
Total RNA was extracted from FACS-sorted cells using TRIzol reagent (Invitrogen™ 15596026) and Direct-zol™ RNA Microprep (Zymo Research R2062) by following manufacturer's instructions. DNA digestion with DNase I was also performed as part of the RNA extraction. cDNA was prepared with 500-1000 ng of total RNA using SuperScript™ IV VILO™ Master Mix (ThermoFisher 11766050) by following manufacturer's instructions. The QuantSeq 3' RNA-Seq Library Prep Kit for Illumina (Lexogen) was used for library construction