GSM7978617: Mouse lungs, post-injury day 14, replicate 1, Visium; Mus musculus; OTHER
Lung tissues were flash frozen in OCT using an isopentane bath with liquid N2. 8 µm sections were acquired directly onto Visium gene expression slides. Visium gene expression slides were processed using the Visium Spatial Gene Expression Slide & Reagent Kit (10X Genomics, cat# 1000184) by the Stanford Functional Genomics Facility. The Gene Expression Slide & Reagent kit was followed per protocol, and used to produce sequencing libraries. The libraries were then sequenced using NextSeq (Illumina), and Bcl files were demultiplexed.