GSM7843165: S.lycopersicum rep1, parasitized; Solanum lycopersicum; RNA-Seq
Total RNA was extracted from tomato roots, using the NucleoSpin RNA kit (MACHEREY-NAGEL, Germany) according to the manufacturer's instructions. Thermo Scientific NanoDrop ND-1000 Spectrophotometer UV/Vis was used to determine the concentration and quality of the RNA. Samples were subjected to cDNA library preparation (poly-A selection) and sequenced (150nt paired-end reads) on an Illumina NovaSeq 6000 system (Novogene Europe, Cambridge, UK). On average 13.9 million reads were obtained per library.