GSM6625477: Experiment 2, iPSC-2 line (Thermo Fisher A18945), 3dpi + ACV, replicate 1, for single-cell RNA-seq; Homo sapiens; RNA-Seq
For single-cell RNA-sequencing, single cell suspensions were generated by enzymatic digestion of organoids, and then fixed using methanol (Alles et al. 2017, doi: 10.1186/s12915-017-0383-5) Single-cell RNA-seq libraries were generated using an extended Dropseq protocol containing a second-strand synthesis step (Wyler et al. 2021, doi: 10.1016/j.isci.2021.102151)