GSM7764414: scTCRseq_MEL03_PBMC; Homo sapiens; OTHER
Sample processing for scRNA-seq and scTCR-seq was performed using the Chromium Single Cell 5' Library & Gel Bead Kit (10x Genomics). Total RNA for RNAseq was isolated using the RNeasy Mini Kit (Qiagen). Single-cell cDNA libraries were prepared using the Chromium Single Cell 5' Library & Gel Bead Kit (10x Genomics). The TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) was used for the fresh tumor tissues and its autologous tumor cell lines. scRNA-seq and RNA-seq