Bacteria isolated from Harvard Forest soils
RC334 was isolated in 2020 on Actinobacteria Isolation Agar with 100 mg L-1 cycloheximide. To extract DNA, the isolate was grown on 10% tryptone soya solid media at 30C shaking at 150 rpm until an OD of 0.5 was reached. gDNA was extracted using the Blood & Tissue DNEasy Kit from Qiagen following manufacturers instructions. Oxford Nanopore sequencing was performed at SeqCenter (Pittsburgh, PA) using R9 flow cells (R9.4.1)