GSM8181759: PDD00017273-treated HCT116, BRD4 ChIP; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. Lysates were clarified from sonicated nuclei and BRD4-histone-DNA complexes were isolated with antibody. Sequencing library was prepared with GenNext NGS Library Prep Kit (Toyobo, LPK-101) from 5 ng for a final sequencing depth of 50 million reads per sample.