GSM6594112: humanizedNeutrophils_WT_1A; Homo sapiens; ATAC-seq
5x10^4 control and 5x10^4 TET2Mut neutrophils were sorted from the same humanized mouse engrafted with a mix of wild-type and TET2Mut hHSPCs. DNA was isolated using ZYMO DNA Clean kit (cat#D4014) Library PCR amplification was done with 9 cycles and double-sided size selection was performed using KAPA Pure beads (Roche, 07983280001). Sequencing was then performed in a HiSeq4000 Sequencing System (Illumina) with a loading molarity of 200pM plus 10% Phix (illumine).