GSM6504837: Bifur_channel_1_ADT; Mus musculus; RNA-Seq
For bulk RNA-seq, in vitro cultured T cells were collected at each time point into RLT-plus buffer, and RNA was extracted with RNeasy Plus Mini Kit (Qiagen). Extracted RNA was further used for cDNA amplification with SMARTA-Seq2 for 12 cycles. For single cell RNA-seq, if no hashtag is added, 7500 cells were loaded for each 10x genomics channel. If using hashtag, we loaded 5000 cells for each hashtag labelled sample for each 10x genomics channel. For bulk RNA-seq, sequencing libraries were prepared with fragmented cDNA amplification products using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced with 2 x 150 paired-end reads. ATAC-seq was performed following the Omni-ATAC protocol (Corces et al., 2017). 10,000 cells were sorted for each ATAC-seq sample preparation.