GSM6450662: Neutrophils Lung Sick 1; Mus musculus; RNA-Seq
RNA from FACS sorted neutrophils and macrophages was extracted with a RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's protocol. Next-generation sequencing libraries were created with 10 ng of RNA from samples with distinct 18S and 28S peaks and RNA integrity number values greater than 7, using an NEBNext Ultra II Directional RNA Library Prep Kit from Ilumina (New England Biolabs) according to the manufacturer's protocol. RNA libraries were pooled and sent for singleend 75 bp sequencing at the Genomics Laboratory (The Walter and Eliza Hall Institute of Medical Research) on a NextSeq 500 next-generation sequencer (Illumina) to obtain ∼20 million reads per sample.