GSM8085526: NSLA_1010; Homo sapiens; RNA-Seq
Total RNA was isolated by RNeasy Mini Kit (Qiagen, CA, USA), according to the manufacturer's protocol. The quality and integrity of the RNA were confirmed by agarose gel electrophoresis and ethidium bromide staining, followed by visual examination under ultraviolet light. Sequencing library was prepared using TruSeq RNA Sample Preparation kit v2 (Illumina, CA, USA) according to the manufacturer's protocols. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, fragmented, and converted into cDNAs. Then, adapters were ligated and the fragments were amplified on a PCR. We used the TruSeq DNA Library Prep Kit (Illumina, San Diego, CA, USA) for TruSeq Stranded Total RNA Sample Preparation Kit (Illumina) for stranded total RNA-seq library, following the manufacturer's instructions. For paired-end sequencing was performed using an Illumina NovaSeq 6000 sequencing instrument, the constructed library was sequenced using a HiSeq 4000 sequencing system, resulting in 2× 100 bp sequencing reads.