GSM7843937: 3D D7, rep1; Homo sapiens; ATAC-seq
Cells from hPSC, mesoderm, cardiac mesoderm, cardiac progenitor, and CM stages were dissociated and processed fresh for droplet-based snATAC-seq. Dissociated cells were stained with DAPI (Thermo Fisher Scientific). In total, 500,000 (DAPI-negative) were sorted and collected using a Sony SH800 sorter. MinElute PCR Purification Kit (Qiagen) and size selection was performed with SPRISelect reagent (Beckman Coulter; 0.55× and 1.5×). Libraries were purified one more time with SPRISelect reagent (Beckman Coulter; 1.5×). barcoded Tn5 transposomes