GSM8408492: DNP2_1_T7; Escherichia coli; RNA-Seq
After mechanical lysis with glass beads, total RNA was extracted using the RNeasy Mini Kit (Qiagen) at times T0, 0.4, 1, 2, 4 and 7 min for the two biological replicates. DNA contamination was removed by DNase I treatment. Total RNA was ribodepeleted using the riboPOOLs kit (Illumina). A paired-end 150 bp sequencing strategy was performed on Illumina PE150 platform (Novogene).