GSM6625095: E. coli W grown in a minimal M9 medium supplemented with L-cysteine replicate 2; Escherichia coli W; RNA-Seq
Cells were treated using the Qiagen RNA-protect bacterial reagent (Hilden, Germany) according to the instruction from the manufacturer. Pelleted cells were stored at -80oC, and after cell resuspension and partial lysis, they were ruptured with a beat beater. Subsequently, the total RNA was extracted using a Qiagen RNA purification kit. After total RNA extraction, ribosomal RNA was removed and the quality was assessed using an Aglient Bioanalyser using an RNA 6000 kit. Paired-end RNA sequencing libraries were prepared by using a KAPA RNA HyperPrep kit from Kapa Biosystems (Wilmington, MA, USA)