title: WGS of N. meningitidis M14569 strain
design: N. meningitidis strains were routinely cultured overnight on Gonococcus (GC) agar medium (Difco) with Kellogg's supplement I or on chocolate agar plates (Biomrieux) at 37C in an atmosphere of 5% CO2. Liquid cultures were grown under the same conditions in Mueller Hinton (MH) broth plus 0.25% glucose. When required, erythromycin (5 g/ml), kanamycin (150 g/ml) or spectinomycin (50 g/ml) were added to culture media. N. meningitidis genomic DNA was extracted from cell suspensions of ON culture plates using the GenElute Bacterial Genomic DNA Kit (Sigma).