title: Metabarcoding of prokaryotes in marine sediments
design: DNA extraction with ZymoBIOMICS DNA Miniprep Kit, amplification of the V3-V5 hypervariable regions of the 16S gene done using the primer pairs 519F_ILMN (5- CAGCMGCCGCGGTAA-3) and 915R_ILMN (5- GTGCTCCCCCGCCAATTCCT-3)