title: Pacbio HiFi WGS sequencing of Boechera falcata
design: The first step in the generation of a SMRTbell library was fragmentation of genomic DNA to appropriate sizes. Then DNA fragments were damage repaired, end-repaired, and A-tailed. The SMRTbell library was produced by ligating universal hairpin adapters onto double-stranded DNA fragments. After the exonuclease and AMPure PB beads purification steps, sequencing primer was annealed to the SMRTbell templates, followed by binding of the sequence polymerase to the annealed templates. T