title: DNA-Seq of MBT-21
design: E. bolteae MBT-21 was isolated from human feces under anaerobic conditions using Anaerobe Basal Broth. The bacterial DNA was prepared using the Quick-DNA Fungal/Bacterial MiniPrep Kit (ZymoResearch, Freiburg, Germany) and the genome was sequenced using the MiSeq Reagent Nano Kit v2 (Illumina, Munich, Germany) according to the manufacturers instructions and sequencing was performed on a MiSeq sequencer (Illumina, Munich, Germany).