title: snRNA-seq of adult human peripheral lobe region of retina
design: Samples included in this study were retinal tissues of donors from the Utah Lions Eye Bank collected within 6 hours post-mortem. All donors were screened for medical history, and only the ones with no of retinal diseases were used in this study. Post-mortem phenotyping with OCT were performed to confirm that there were no drusen, atrophy, or any other disease phenotypes on retina. With 4mm and 6mm disposable biopsy punches, macula and peripheral retina were collected and flash frozen in liquid nitrogen, and stored at -80C before nuclei isolation. Nuclei for snRNA-seq were isolated by fresh-made pre-chilled RNase-free lysis buffer. The frozen tissue was resuspended and triturated in lysis buffer and homogenized with a WheatonTM Dounce Tissue Grinder. Single cell Gene Expression Library was prepared according to Chromium Next GEM Single Cell 500 3' Reagent Kits v3.1 (10x Genomics).