title: PacBio HiFi of Perdita meconis: male
design: From the entire DNA extraction, we prepared a single SMRTbell library using the SMRTbell library preparation kit following manufacturers protocols for low input samples. DNA was sheared to 15-20 kb using a Megaruptor 2 (Diagenode, Denville, NJ, USA) prior to library preparation, and the final library was sized with Ampure PB beads to remove fragments less than 3 kb in length. No additional sizing was performed in order to retain as much DNA as possible. The entire library was quantified using Qubit HS DNA reagents, sized on a Agilent Fragment Analyzer to determine molar concentration and then bound and loaded for sequencing on a single 8M cell on a Pacific Biosciences Sequel IIe system