title: gut metagenome of rattus norvegicus: female alcohol vehicle
design: DNA was extracted from frozen fecal samples using the QIAamp PowerFecal Pro DNA kit according to manufacturers instructions (Qiagen-USA) and sent to Florida State University Next-Generation Sequencing Library Facility for the preparation of a total of 123 (n=5-6 per group x 3 timepoints) 16S rRNA gene sequencing libraries targeting the V3-V4 region and their subsequent sequencing using the Illumina MiSeq instrument (2x250 bp, MiSeq v2 reagents, Illumina, San Diego, CA, USA).