title: 18S metabarcoding of 1L Sterivex filtered deep seawater from Niskin Darya: deep seawater
design: 1L of deep seawater collected with Niskin Darya was filtered through a 0.2 micron Sterivex. DNA was extracted using the Qiagen PowerSterivex kit. Primers targeting the 18S rRNA gene (Amaral-Zettler et al. 2009) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).