title: 16S rRNA amplicon seq: SynCom and P. veronii in Toluene-contaminated SE with Phosphite, 24 h
design: DNA was purified from thawed cell pellets using the DNeasy PowerSoil Pro kit (Qiagen) and library preparations were performed according to the Illumina 16S Metagenomic Sequencing Library protocol (https://support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/16s/16s-metagenomic-library-prep-guide-15044223-b.pdf) for the V3 to V4 region of the 16S rRNA gene. The gene library was indexed using Nextera XT Index Kit Set A and B (v2, Illumina Inc., USA) and ;braries were supplied with 40% PhiX control.