title: Illumina NovaSeq 6000 Paired-end sequencing of wastewater sludge metagenome
design: Metagenomic DNA was fragmented by sonication on a Covaris E210 device. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina. Sequencing was performed using the NovaSeq6000 sequencer, paired-end (PE) technology, 2x150nt, using NovaSeq 6000 S4 Reagent Kit v1.5 300 cycles.