title: metagenome of human stool: S5
design: DNA was extracted from the FOBT cards at the Laboratory of Precision of Environmental Health at Columbia Mailman School of Public Health following adapted protocols for DNA extraction from bloodspots and solid stool. DNA was extracted from the eluent using the QIAamp PowerFecal Pro kit following the manufacturers protocol (Qiagen, Hilden, Germany). DNA  was normalized to 30 ng/?l and sent to the New York University Langone Genome Technology Center (New York, USA) for shotgun metagenomic sequencing. Technicians pooled libraries with Illumina Flex (San Diego, CA, USA) library preparation and sequenced the specimens with an Illumina NovaSeq 6000 using an S2 flowcell to obtain 2 x 150bp paired-end reads.