title: Whole genome sequence of the Theileria annulata infected cells:TA3
design: Whole genome sequencing  (WGS)  library  was  prepared  with  Illumina - compatible  NEXTflex  Rapid  DNA  sequencing  kit at  Genotypic  Technology  Pvt.  Ltd.,  Bangalore , India. Briefly, DNA was sheared using Covaris S2 sonicator to  generate approx.  fragment size distribution from  200 bp to 400 bp . The fragment size distribution was  checked on  Agilent  TapeStation  and  subsequently  purified  using  High prep magnetic  beads  (Magbio) .  Purified fragments were end - repaired, adenylated and ligated to  Illumina multiplex barcode adaptors as per  NEXTFlex  Rapid DNA sequencing kit protocol. Adapter - ligated DNA was purified  using High prep beads.  Resultant fragments were amplified  for  8 cycles of PCR using Illumina - compatible primers provided in the NEXTFlex Rapid  DNA sequencing  kit. Final  PCR  product  (sequencing  library)  was  purified  with High prep beads ,  followed  by  library  quality  control  check .  Illumina - compatible  sequencing  library  was quantified  by  Qubit  fluorometer and  its  fragment  size  distribution  was  analyzed  on  Agilent  2200  Tapestation.