title: RNA-seq of Pachycladon cheesemanii : non-treatment
design: Total RNA was extracted from mature leaves using a Quick-RNA MiniPrep Kit and treated with DNase I to remove genomic DNA contamination. Purified untreated and treated plant RNA was used to generate paired-end sequencing libraries, including 12 A. thaliana and 12 P. cheesemanii samples (three biological replicates for untreated plants and each stress treatment in each plant species). Following library quality control, the libraries were sequenced on Illumina HiSeq X Ten platform. PE library construction and Illumina sequencing were performed by Novogene Limited