title: Amplicon sequencing of soil bacterial community
design: DNA was extracted from the soil using the Qiagen Powersoil kit. The 16S gene was amplified using the primer pair 515F and 806R following the Earth Microbiome Project protocol. Replicates were pooled and then pooled libraries were sequenced on an Illumina MiSeq (Reagent Kit V2) with 2x150 bp paired end reads.