title: Capture sequencing of: Stichodactyla gigantea
design: Up to 1000ng of genomic DNA was used for library preparation, DNA was sheared to a target fragment size of 400-800bp using a Covaris Ultrasonicator. Library preparation was performed using a Kapa Hyper Prep Kit for bait-capture sequencing, with universal Y-yoke oligonucleotide adapters and iTru dual-indexed primers (Glenn et al. 2016). Libraries were pooled into equimolar ratios (100ng) and target enrichment was performed using MyBaits v.IV protocol using a 500 ng/rxn concentration of baits (Glon et al. 2021). Bait-capture enriched libraries were sequenced at Arbor BioSciences on an Illumina NovaSeq using 150bp paired-end sequencing.