title: RNA seq of cecal tissue mus musculus
design: intestinal lamina propria and epithelial cells isolated prior to sequencing. RNA was extracted from lamina propria cells and epithelial fraction isolated from the cecum-colon using RNeasy Plus Mini Kit (Qiagen). An RNA library was prepared using the Illumina TruSeq RNA sample preparation kit and sequenced with the Illumina Hiseq2000 using the TruSeq RNA v.2 protocol. Illumina CASAVA v.1.8.2 was used to generate FASTQ files