title: Mouse-06
design: DNA was quantified using the Quant-iT PicoGreen kit (Invitrogen). 16S rRNA genes were amplified in triplicate using 30ng of extracted DNA as template. The V4 region was amplified using 515F and 806R primers with Illumina adapters and bar codes on the 3 end. PCR products were pooled for quantification and visualization using the QIAxcel DNA Fast Analysis kit (Qiagen). Pooled PCR products were then processed using the Mag-Bind RxnPure Plus kit (Omega Bio-tek) according to the manufacturers protocol, to include only sequences between 250-400 bp. The cleaned pool was sequenced on the MiSeq using a v2 2x250 base pair kit (Illumina). Sequences were processed following a standard pipeline and classified using Mothurs version of the Ribosomal Database Project classifier (Mothur v1.39.5).