title: mtDNA of the Satanas beetle, Dynastes satanas
design: This study involved sequencing of genomic DNA extracted from Dynastes satanas. The sequencing libraries were prepared from 1g of DNA, fragmented using Covaris M220 to 300~500bp. End repair, A-tailing, and indexing were performed using the TruSeq Nano DNA Sample Prep Kit. Libraries were enriched with 8 cycles of PCR and gel purified using 2% certified low-range ultra agarose gel. Quantification was done using TBS380 (Picogreen) and libraries were pooled and sequenced on an Illumina NovaSeq platform using paired-end 2x150bp reads.