title: Mol16S with fish blocker/MiFish12S amplicon sequencing of plankton tow: April 2018 P38 vertical KA Ohio
design: Single index used for independently-amplified reactions: Mol16S primers with competing fish blocker primers (Klymus et al., 2017) (35 cycles, 58 degC annealing), MiFish12S primers (Miya et al., 2015) (35 cycles, 65 degC annealing). Indexing with 8 cycle PCR; MiSeq PE 2x301 bp at the Molecular and Cellular Imaging Facility (Ohio State University).