title: DNA sequencing of Canis lupus familiaris: high-grade oligodendroglioma cell line
design: Mo-HO cells were grown to ~70% confluence, then DNA was isolated using DNeasy Blood and Tissue Kit (QIAGEN).  DNA concentrations were measured by a Qubit 3.0 Fluorometer (Thermo Fisher Scientific) and A260/A280 absorption ratios were measured on a NanoDrop OneC Spectrophotometer (Thermo Fisher Scientific). A total of 1g genome DNA was fragmented by M220 Focused-ultrasonicator for a 550bp targeted insert size (Covaris). DNA sequencing libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). The final library concentration was accessed with Qubit 3.0 Fluorometer, and the size distribution was checked on a Perkin-Elmer LabChip GX Touch HT machine (PerkinElmer, MA After).