title: ddRAD-seq of Mimosa ramulosa individual X1
design: 10 microliters of 15ng/uL DNA diggested with the enzymatic pair MboI-PstI. Adapters (Peterson et al., 2014) and indices (Lange et al., 2014) were added after digestion. The two indexed samples were pooled in equimolar amounts (17uL) and subjected to fragment selection (450-550 pb) by 1,5% low melting agarose (BioRad) gel electrophoresis.