title: Co-culture_Day_2 Replicate 2
design: PCR amplification of targeted regions was performed by using specific primers connecting with barcodes. The PCR products with proper size were selected by 2% agarose gel electrophoresis. Same amount of PCR products from each sample was pooled, end-repaired, A-tailed and further ligated with Illumina adapters. Libraries were sequenced on a paired-end Illumina platform to generate 250bp paired-end raw reads. Primers used were 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3)