title: 16S Amplicon v3-v4 sequencing: hypolith
design: DNA was extracted from 0.75 g of soil using the QIAGEN DNeasy PowerSoil kit and sequenced using the v3-v4 region  forward primer: 5'-CCTACGGGNGGCWGCAG-3' and reverse primer 5'-GACTACHVGGGTATCTAATCC-3'