title: Nanopore DRS of Tetrahymena:growth
design: Tetrahymena wild-type strains (SB210 ) were obtained from the Tetrahymena Stock Center (http://tetrahymena.vet.cornell.edu). Cells were grown in SPP medium at 30C. Total RNA was collected using the RNeasy Plus Mini kit (Qiagen, 74134). The quality and concentration of RNA samples were analyzed by 1% agarose gel electrophoresis and Qubit3.0 Fluorometer (Thermo Fisher Scientific). Direct RNA sequencing libraries of SB210 were constructed according to manufacturer-recommended protocols and sequenced by Novogene Co. Ltd (Beijing, China).