title: Single cellwhole genome sequencing of tumor sample from high-grade serous ovarian cancer patient.: Sample SHAH_H000162_T04_01_DLP01
design: scWGS libraries were prepared using the DLP+ protocol.  In brief, single cell suspensions were fluorescently stained with a combination of CellTrace CFSE Cell Proliferation Kit (ThermoFisher) and LIVE/DEAD Fixable Red Dead Cell Stains. Cells were dispensed into a 72x72 open nanowell array (TakaraBio SmartChip) using a cell dispensing system that combines a contactless piezoacoustic dispenser with brightfield and fluorescent imaging to select live cells and exclude doublets (cellenONE, Scienion). Isolated cells (target 800-1600 per sample) were be subjected to cell lysis, Tn5 based tagmentation to fragment DNA and incorporate illumine adapters, PCR to incorporate cell specific barcodes, and subsequently pooled and illumina sequenced on the Novaseq 6000 with 2x150bp reads to ~0.05X per cell coverage.