title: RNA-Seq of mouse live
design: Briefly, mRNA was enriched from 1  g total RNA by polyA selection using oligo (dT) beads, then fragmented by fragmentation buffer, and reverse transcribed using random hexamer-primers to generate first-strand cDNA. Second-strand cDNA was then generated using RNase H and DNA polymerase. Next, cDNA was subjected to purification by AMPure XP beads, end repair,A-base addition and ligation of the Illumina-indexed adaptors.