title: IBD2KO-A1-NHA_Input
design: MACs of HA-tagged strains were purified and digested following established protocols (Chen et al., 2016). Approximately 5  107 purified MACs were digested by Micrococcal Nuclease (MNase, NEB) (200 U/ml, 25C, 15min) to generate mononucleosomes. After extraction in T150 buffer, solubilized chromatin was immunoprecipitated with Pierce anti-HA magnetic beads (Thermo Fisher Scientific, I2149) at 4C overnight (Cuddapah et al., 2009) and HA-tagged proteins were eluted with the HA peptide (Sigma, E6779).