title: hepg2_hTF_lib_SS_t25_rep_3
design: Cells were treated with BODIPY FL LDL in optiMEM. Cells were trypsinized and sorted into bins. After sorting, genomic DNA was isolated from cells and used to amplify the U6-3 to gRNA hairpin region with different in-line barcodes. PCR2 was performed to add full-length Illumina sequencing adapters using internally ordered primers with equivalent sequences to NEBNext Index Primer Sets 1 and 2 (New England Biolabs).