title: Single-cell ATAC-Seq of H9-P2 sample from hESC H9 passaged 200s
design: Nuclei were isolated from cells using the Nuclei Isolation Kit: Nuclei EZ Prep (NUC101-1KT) according to the manufacturers instructions. Sequencing library was prepared using Chromium Next GEM Single Cell ATAC Reagent Kit (10x Genomics; PN-1000176, v1.1). About 8,000 nuclei were isolated for Tn5 transposition, nuclei barcoding, and library construction. Quality checked libraries were sequenced using the Illumina Sequencing platform.