title: Illumina sequence data: Evaluation of abundance and diversity of bacterial population in the rhizosphere of citrus trees
design: DNA was isolated from citrus rhizosphere samples using a kit. to create a library, two consecutive PCR reactions were used to amplify and barcode the PCR products. In the first PCR, primers 515 F and 926 R were used to amplify the variable regions 4 and 5 of the 16S rRNA gene of the samples. To perform the secondary PCR, 2 l of the primary PCR product was used as a template. In this step, exclusive primers of Illumina Company were used, with 8-nucleotide barcode sequences and adapter sequences added at the end.