title: Metabarcoding of nematodes of Store Mosse
design: Index PCR was performed where unique indices were attached to amplicons of each sample. Library size distribution was checked by running on Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip. Quantification of the library was performed using qPCR according to the Illumina qPCR quantification protocol guide. The samples were then multiplexed, before Illumina MiSeq 2x300 bp sequencing.