title: HiFi sequencing of Entacmea quadricolor: adult pedal disk
design: HMW DNA was extracted using PacBio Nanobind kit. DNA was purified with Monarch Nucleic Acid Purification Kit prior to shearing on Megaruptor 2 at 20kb. Library was constructed using SMRTbell Prep Kit 3.0 with barcoded adaptors, and size-selected on BluePippin to remove DNA fragments less than 10kb. Library was bound to Revio polymerase, then sequenced with Revio Sequencing Plate and Revio cell on the Revio instrument.