title: ATAC-seq for human T cells: Sample HIV15
design: ATAC-seq for human T cells; PMCID: PMC6878759; sorted T cells were pelleted and resuspended in 50uL lysis buffer (10 mM Tris-HCl [pH 7.4], 10 mM NaCl,3 mM MgCl2, 0.1% IGEPAL CA-630).  Transposition reaction was carried out immediately in a 25uL reaction with 1.25uL of Tn5 transposase (Illumina) in a 37oC water bath for 37 minutes to tag and fragment accessible chromatin. Tagmented DNA was purified using a MinElute Reaction Cleanup Kit (QIAGEN) and amplified with 12 cycles of PCR. Libraries were purified using Agencourt AMPure XP kit (Axygen). Sample quality was determined using a Tapestation 2200 system (Agilent).  Library concentrations were determined using Qubit dsDNA HS Assay Kit (Invitrogen). Libraries were paired-end sequenced on a NextSeq 550 (Illumina).