title: 16s rRNAseq for macaque
design: We extracted microbial genomic DNA. PCR targeted the 16S rRNA gene V3-V4 re-gion using 338F and  806R. Purified PCR products underwent Bioo Scientifics NEXTFLEX Rapid DNA-Seq Kit li-brary prep (Austin, TX, USA). Illuminas PE300/PE250 platforms were sequenced.