title: 16srRNA-seq of pet cats
design: Total DNA was extracted using the NucleoSpin DNA Insect Kit (Macherey-Nagel, Dren, Germany), following the manufacturers instructions. The 16S rDNA V4 region was amplified by polymerase chain reaction (PCR) using the primers 515F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGCCAGCMGCCGCGGTAA-3) and 806R (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGG TWTCTAAT-3)