title: Whole genome sequencing of Cypha longicornis
design: DNA was isolated from two specimens using complete tissue homogenization with the Qiagen DNeasy Blood and Tissue extraction kit (Qiagen) following the manufacturers protocol with slight modifications as follows. Tissue was homogenized in the ATL lysis-proteinase K solution and incubated for 4 h or overnight at 56 C. The tissue solution was incubated in RNaseA (Qiagen) for 2 min followed by the manufacturers protocol. Two rounds of DNA elution were performed with 100 l warmed elution buffer (50 C) each round. DNA was concentrated using the Monarch PCR and DNA Cleanup kit (New England Biolabs) with warmed elution buffer. DNA quantity was assessed using the Qubit High Sensitivity dsDNA kit (Thermo Scientific) and DNA integrity was assessed visually with gel electrophoresis. Illumina paired-end sequencing libraries were prepared using the NEBNextUltraIIFS kit library kits (paired-end 150bp reads, average insert size 155  105 bp) following the manufacturers protocol, quantified with Agilent Bioanalyzer High Sensitivity DNA kit and sequenced on HiSeqX by Fulgent.