title: CRISPR/Cas9 mutation readout on the pathway reporters in K562 TRIP RSTP2_clone5 on sample: K562_ddCas9_RSTP2_clone5_robotics_screen_P5_H8_TENT4A_LBR2_72h_B4_R2_Run1
design: CRISPR/Cas9 mutation readout on the pathway reporters in K562 TRIP RSTP2_clone5 after individual DNA repair protein knock-outs. Five days after knock-out induction, a second gRNA is transfected to induce DSB in 19 pathway reporters in parallel. Three days later, cells are harvested for mutation readout PCR.