title: 16S metabarcoding of the surface of a smear-ripened cheese: PCR2 negative control (water)
design: The second PCR amplification (12 cylces) was performed directly onto molecular biology-grade water (PCR negative control). After purification and quantification, all libraries were pooled with equal amounts and the mixture was loaded onto the Illumina MiSeq cartridge according to the manufacturers instructions using MiSeq Reagent Kit v3 (2  250 bp paired-end reads).