title: Subsurface Soil active metagenome of farmland
design: Complementary DNA (cDNA) was synthesized from the extracted RNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) following the manufacturer's instructions. Library preparation and sequencing of using the 341F and 805R primer set targeting the V3V4 region of the 16S rRNA gene on an Illumina Miseq was performed by Macrogen (Seoul, South Korea).