title: Amplicon-seq of PFS library of target plasmid encoding kanamycin resistance used in PFS assay
design: Electrocompetent E coli harboring a randomized PFS library on the 5' UTR of an antibiotic resistance gene was generated and transformed with a plasmid encoding Cas13an2 and crRNA. Region of interest bearing the PFS library on the plasmid was amplifed via PCR following antibiotic selection in E coli. Surviving cells were transformants that had a target plasmid whose target flanking sequence was not preferred by Cas13an2.