title: gut microbiome of parrot
design: 16S rRNA sequencing libraries targeting the V3-V4 regions were prepared. PCR amplification used KAPA HiFi HotStart ReadyMix and ARAClean Beads for purification. Initial PCR employed region-specific primers compatible with MGI index and sequencing adapters. After purification, a second PCR with MGIEasy UDB Primers Adapter Kit A was performed. PCR products were visualized via gel electrophoresis and quantified using a Qubit dsDNA HS Assay Kit. Library circularization and DNA nanoball (DNB) formation were done with MGIEasy modules. The final library was quantified and sequenced on the MGIseq system with 300 bp paired-end reads.