title: EU-RNA-Seq of SF3B4 KD HeLa: 20 minutes after labeling
design: Based on Hela S3 cells, >80% knockdown of SF3B4 gene was obtained (sense siRNA: GCAGUACCUCUGUAACCGU, antisense siRNA: ACGGUUACAGAGGUACUGC). The cell culture was incubated with 5-ethynyl uridine (EU) for 20 minutes/ 24 hours. Total RNA was extracted using QIAzol lysis reagent. Nascent RNA labeled with EU was purified using Click-iT Nascent RNA Capture Kit. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.