title: 1st_Stage2
design: DNA extraction was performed using a FastDNA SPIN Kit for Soil (MP Biomedicals, LCC). Nanodrop spectrophotometer (SPECTROstar Omega  BMG Labtech, DE) was used to determine the quality and concentration of extracted DNA. DNA was sequenced on Illumina Novaseq 6000 platform (2  150, paired end) carrying out library preparation with Nextera DNA Flex Library Prep Kit (Illumina Inc., San Diego CA)