title: RNA-seq of mus musculus: Control
design: RNA-seq analysis was performed using 6 day-expanded mouse T cells or 12 day- expanded human T cells cultured in the presence or absence of mannose. Briefly, total RNA was extracted using Trizol reagent (Takara) and subsequently was conserved in RNase-free tubes by Liquid nitrogen quick-freezing method. Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was used as input material for the library preparation. The prepared library quality was initially quantified by Qubit2.0 Fluoromete and then detected by Agilent 2100 bioanalyzer. After the library was qualified, the different libraries were sequenced by the Illumina NovaSeq 6000 to generate the end reading of 150bp pairing (Novogene).