title: WGBS of human Th17 lymphocytes
design: Initially, genomic DNA spiked with lambda DNA was fragmented to sizes ranging from 200 to 400 base pairs. These fragments underwent bisulfite treatment to generate single-stranded DNA (ssDNA). Following this, methylation sequencing adapters were ligated, and double-stranded DNA synthesis was carried out to construct the library using the Accel-NGS Methyl-Seq DNA Library Kit for Illumina. Subsequently, size selection and PCR amplification were employed to prepare the library, which was then quantified using Qubit and real-time PCR. The size distribution of the quantified libraries was assessed using a bioanalyzer. Finally, the quantified libraries were pooled and sequenced using Illumina platforms.