title: Single cell sequencing of musmusculus: young  cochlear tissue
design: Cochlear tissue was transported into the iced DMEM (Thermo Fisher Scientific, Waltham, MA). After being washed with the PBS for three times, cochlear tissues were minced into <1 mm3 size and transferred into a 50 ml tube (BD Falcon, Franklin Lakes, NJ) containing DMEM supplement. Briefly, harvested cochlear tissues were digested with 0.2% collagenase type I for 20 min and then digested with 0.25% trypsin for 10min. All cells were passed through 40 m strainers and centrifuged at 300g at 4C for 6 minutes. The cell pellet was harvested and re-suspended in iced DMEM prior to barcoding on the chromium genomics platform.