title: small RNA-seq of E coli with plasmid encoding Cas13an10 native locus
design: NEB10B cells transformed with cas13 native locus plasmid was grown on LB plate overnight. Colonies were innoculated to liquid LB and grown culture to OD 0.6, and induced with arabinose. Following induction cells were allowed to grow overnight. RNA was extracted via hot formamide extraction, and sequencing library was prepared using Collibri RNA-seq kit. Size selection was performed on 4% agarose gel to sequence amplicons ranging from ~150 to ~450 in size.