title: RNA-Seq of Homo sapiens: 45X CD4
design: Cells were negatively enriched from PBMCs on the same day as blood draw and frozen at -80C until RNA extraction with TRIzol and acid phenol:chloroform, followed by ethanol precipitation. 2uL of a 1 to 1000 dilution of ERCC Spike-Ins were added per 100ng of RNA.   Libraries were prepared using the Roche Kapa mRNA HyperPrep Kit v2 according to manufacturers instructions. Libraries were size-selected using 2 percent agarose gels on the Sage Science PippinHT system with a capture window of 300-600 bases.