title: Short Read Illumina ITS Sequencing of Nasal Swab from Chronic Rhinosinusitis Patients
design: DNA extraction was performed using the QIAGEN DNeasy PowerSoil Pro kit(QIAGEN, Hilden, Germany) at the Australian Genomic Research Facility(AGRF, Melbourne, Australia). Extracted DNA from a common swab was divided and allocated to three separate sequencing pathways: fungal ITS; bacterial 16S (V3-V4) NGS and full length 16S. ITS and 16S NGS amplification and sequencing steps were performed by AGRF in accordance with local protocols (Supplementary 2), applying a 300-base pair chemistry, 2-stage PCR and sequencing utilising Illumina Miseq(Illumina Inc., San Diego, California).