title: RNA-seq for human T cells: Sample HIV15
design: RNA-seq for human T cells; PMCID: PMC6878759; Sorted T cells were pelleted and resuspended in Buffer RLT Plus (QIAGEN) with 10% 2-Mercaptoethanol (Sigma).  Total RNA from each sample was isolated using the RNeasy Plus Micro Kit (QIAGEN). RNA integrity was determined using a 2100 Bioanalyzer (Agilent).  Reverse transcription and cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech).  cDNA qualities were determined by a Tapestation 2200 system (Agilent) and concentrations were determined using Qubit dsDNA HS Assay Kit (Invitrogen).  DNA libraries were prepared using Nextera XT Library Prep Kit (Illumina) and sequenced on NextSeq 550 (Illumina).