title: 16S rRNA-Seq of environmental metagenome: aerosol sample
design: Aerosol filter sample collected from sea spray generation mescocosm experiments. For experimental details see corresponding publication. Extraction with Qiagen PowerSoil kit. Triple PCR amplification of the V3-V4 region of the 16S rRNA gene using the Bac341F primer (50-CCT ACG GGN GGC WGC AG-30) and the Bac805R primer (50-GAC TAC HVG GGT ATC TAA TCC-30) with overhang adapters. The PCR product was cleaned AMPure XP beads and normalized to 4 ng/l.