title: PHN05
design: The purified PCR products were assessed using the Agilent 2100 Bioanalyzer (Agilent, USA) and Illumina's library quantification kit (Kapa Biosciences, Woburn, MA, USA). A library concentration exceeding 2nM was deemed acceptable. The qualified computer sequencing libraries, which featured non-repeatable index sequences, were subjected to gradient dilution, mixed in accordance with the required sequencing volume, and denatured into a single strand via NaOH for computer sequencing. The NovaSeq 6000 sequencer was employed for 2250bp double-ended sequencing, utilizing the NovaSeq 6000 SP Reagent Kit (503 cycles).