title: Soil microbial community: RAWSOIL
design: Genomic DNA of the soil samples were extracted using a soil DNA isolation kit (OMEGA Biotek Inc., USA) following the protocol as recommended by the manufacturer. The DNA was quantified by a NanoDrop spectrophotometer (ND-1000, USA), and then stored at 20 C for further analysis. 16S rRNA genes were amplified with prokaryotic primers 338F-806R (Wagg et al., 2019). The amplified products were purified and then sequenced on the Illumina Miseq pyrosequencing platform by Majorbio Bio-pharm Biotechnology Co., Ltd (Shanghai, China).