title: HiFi reads of Mytilus galloprovincialis: adult female adductor muscle
design: The genomic DNA was extracted using QIAGEN Genomic-tip 100/G (QIAGEN, Germany) according to the manufacturer's instructions. DNA degradation and contamination were monitored by pulsed field gel electrophoresis. DNA concentration and purity were respectively analyzed by Qubit DNA Assay Kit in Qubit 2.0 Flurometer (Invitrogen, USA) and Nanodrop 2000 (Thermo, USA). A total amount of 8 g DNA was used as input for library preparations. HiFi SMRTbell library was generated using SMRTbell Express Template Preparation Kit 2.0 following manufacturer's recommendations and sequenced the 15 to 20 kb average HiFi SMRTbell library. The DNA library was loaded on the flow cell with sequencing buffer and loading beads. Use DNA Sequencing Reagent Kit and follow the manual to load libraries in SMRT Cells.