title: Tiled RT-PCR of SARS-CoV-2 spike gene from wastewater.
design: Total nucleic acid from solids-depleted wastewater liquid (Ceres concentrate) treated with TurboDNase, concentrated, reverse transcribed with SuperScript IV VILO, amplified with ARTIC v4.1 spike primers using NEB Q5, converted into Illumina-compatible libraries using NEB Ultra II DNA kit, and sequenced on an Illumina system 250PE or 300PE.