title: 16S rDNA metabarcoding of microbial communities of biofilms in the Shulgan-Tash Cave in the Southern Ural.
design: DNA was extracted by the DNeasy PowerBiofilm Kit (Qiagen, Germany) with FastPrep-24 homogenizer (MP Biomedicals, USA) according to the protocol provided by the manufacturer. The amount of DNA was assessed using Qubit (Invitrogen, USA) and Qubit dsDNA High Sensitivity Kit (Invitrogen, USA). Archaeal V3 to V4 variable regions of 16S rRNA genes were amplified with the universal primers Arch349F (5'-GYGCASCAGKCGMGAAW-3') and Arch806R (5'-GGACTACVSGGGTATCTAAT-3'). Sequencing was performed using Illumina MiSeq platform and MiSeq Reagent Kit V3 2300 bp (Illumina) according to the Illumina workflow (Illumina protocol, Part #15044223, Rev. B).