title: Whole body transcriptome of Holobus
design: RNA from a single specimen was extracted using a combination of Trizol and Rneasy extraction kit (Qiagen). RNA quantity and quality was assessed using the NanoDrop. Illumina paired-end sequencing libraries were prepared using the Illumina TruSeq RNA library kits (paired-end, 150bp reads) following the manufacturers protocol and sequenced on HiSeqX by Fulgent.