title: DNA-Seq of Elophila obliteralis larva
design: High molecular genomic DNA was extracted from the thorax of E. obliteralis using the Quick-DNA HMW MagBead Kit (Zymo Research). Extractions with at least 1 g of high-molecular-weight DNA (>40 Kbp) were sheared, and the BluePippin system (Sage Science, Beverly, MA, USA) was used to collect fractions containing 15-Kbp fragments for library preparation. Genomic libraries were prepared with DNA following the instructions of the SMRTbell Express Template Prep. Kit 2.0 (PacBio, Menlo Park, CA, USA). The library was sequenced on two 8M SMRTcells with 30-hour movie times in ccs (circular consensus sequencing) using the PacBio Sequel II system.