title: RNA-Seq of Trichoderma reesei: d60282, inducing carbon source, replicate2
design: mRNA with polyA structure was enriched from total RNA using Oligo (dT) magnetic beads, and broken into fragments of approximately 300 bp using ion disruption. Using mRNA as a template, the first strand of cDNA was synthesized using 6-nt random primers and reverse transcriptase, and the second strand of cDNA was synthesized using the first strand of cDNA as a template. After the library construction is completed, PCR amplification is used to enrich the library fragments, and then the library was selected based on the fragment size of 450 bp.