title: The full-length transcriptome sequencing of a  mix sample on PacBio Sequel ll
design: Equal quantities of RNA from five tissue samples, i.e. root, young trophophyll, young sporophyll, mature trophophyll, and mature sporophyll, were used for PacBio cDNA library construction. Briefly, a total of 1 g RNA from the mixed RNA sample was reverse transcribed using the Clontech SMARTer polymerase chain reaction (PCR) cDNA Synthesis Kit and oligo (dT) to obtain the first-strand cDNA. The second strand was synthesized and amplified with the 5 PCR Primer to generate barcode full-length cDNA. Size selection was carried out on a BluePippin Size Selection System protocol with two size bins for the mixed sample: 1-4 kb and > 4 kb. An SMRT cell was sequenced on the PacBio Sequal platform.