title: RNA-Seq of Solanum lycopersicum: tZ treated with NaCl (2h)
design: cDNA synthesis from fragmented mRNA was carried out using random hexamer primer and M-MuLV RT for first strand synthesis and DNA polI and RNaseH for second strand synthesis. Overhangs were converted to blunt ends using exonuclease/polymerase activities. 3' end was adenylated and hairpin loop adapters were ligated. Library fragments were purified with AMPure XP system and PCR was performed with Phusion High-Fidelity DNA polymerase.