title: MiFish12S amplicon sequencing of plankton tow: September 2018 P8 oblique BS Ohio
design: Amplification with MiFish12S primers (Miya et al., 2015) (35 cycles, 65 degC annealing); Indexing with 8 cycle PCR; MiSeq PE 2x301 bp at the Molecular and Cellular Imaging Facility (Ohio State University).