title: Ribo-seq of mus musculus: heart tissues
design: The Ribo sequencing service and the RNA sequencing service were provided by ShuPu (Shanghai) BIOTECHNOLOGY LLC using the Illumina sequencer according to a previous report13,14. For Ribo-seq, the lysate was digested using RNase to remove RNA that is not protected by ribosomes. High-density sucrose centrifugation was performed to obtain a single ribosome followed by the extraction and purification of RPFs. cDNA library was then constructed and sequenced after quality control. Products. Products between 146-154 bps were subjected to the purification and recovery (corresponding to 26-34 nt mRNA covered by ribosomes). 51 cycles were sequenced on the IIlumina NextSeq 500 sequencer according to the supplier's instructions. The library_source is Translatomic.