title: Liver transcriptome of cholecystectomized mice fed High-fat diet
design: Total RNA was extracted using TRIzol (Invitrogen) from in mouse liver. RNA concentration and purity were detected using a NanoDrop200, and RNA integrity was detected using 1% agarose gel electrophoresis. mRNA was enriched using magnetic beads with Oligo(dT). The enriched mRNA was fragmented using fragmentation buffer. The sequencing library was constructed using a Truseq TM RNA sample prep kit (Illumina, USA). RNA-Seq was conducted using a Hiseq Xten sequencing platform (Illumina, USA).