title: 18S metabarcoding of 10L prefilter filtered shipboard freshwater from Niskin Sedna: freshwater control
design: 10L of water shipboard freshwater added to bleached Niskin Sedna was filtered through a 0.8 micron prefilter. DNA was extracted using the Qiagen PowerSterivex kit. Primers targeting the 18S rRNA gene (Amaral-Zettler et al. 2009) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).