title: RNA-seq of Saccharomyces cerevisiae
design: To conduct the transcriptional analysis, strain SCSA001 was cultured in Delft MM with sole yeast extract or yeast extract and 2% methanol at 30 C, 220 rpm for 48 h. 2 ml samples were rapidly centrifuged at 4  and 10000 rpm for 2 min, which was washed with cold 1 DEPC water and centrifuged twice. The supernatant was fully removed and then frozen in liquid nitrogen for 15 min. Then, the sample was stored at -80. Comparative RNA sequencing analysis was carried out by Shanghai Personalbio Biotechnology Co., Ltd. By using Illumina high-throughput sequencing platform. All treatments were conducted in batch culture in triplicates.