title: Biopsy sample was enzymatically digested within 3 hours of collection with collagenase/dispase to generate a single cell suspension and submitted to single cell RNA sequecing as described in the design.
design: Cells were loaded on a 10x Chromium controller and processed following the NextGEM v3.1 single-index 3' Chemistry (10x Genomics, CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D). Libraries were sequenced on an Illumina HiSeq with the following read configuration: R1 (cell barcode and UMI): 28bp, i7 index: 8bp, R2 (insert): 96bp.