title: EU-RNA-Seq of HeLa: 24 hours after labeling
design: HeLa cell culture was incubated with 5-ethynyl uridine (EU) for 24 hours. Total RNA was extracted using QIAzol lysis reagent. Nascent RNA labelled with EU was purified using Click-iT Nascent RNA Capture Kit. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.