title: RNA-seq for the parental UMUC3
design: VNTR6-1 KO lines were made by treating UMUC3 parental lines with dual-guided CRISPR/Cas9 targeting VNTR6-1 regions within the TERT gene. RNA from two replicates each of parental, VNTR2-1 KO, and VNTR6-1 KO was purified, and 200 ng was submitted for RNA-seq.