title: Shotgun seq
design: Nextera DNA Flex kit (Illumina, San Diego, CA) has been used for library preparation following themanufacturers instructions. Both input and final libraries were quantified by Qubit 2.0 Fluorometer(Invitrogen, Carlsbad, CA) and quality tested by Agilent 2100 Bioanalyzer High Sensitivity DNA assay(Agilent technologies, Santa Clara, CA).Libraries were then prepared for sequencing and sequenced on NovaSeq 6000 in paired-end 150mode.