title: Amplicon-seq of spacer library from plasmid encoding Cas13an2 used in mismatch tolerance study
design: E coli were co-transformed with Cas13an2 plasmid library with variable spacers and a fixed target plasmid with anbiotic resistance gene. Following antibiotic selection, region of interest from plasmid in E coli was amplifed via PCR. Surviving cells were transformants that had a Cas13an2 guide RNA pairing whose spacer had reduced activity. Use of arabinose inducible promoter allowed testing in induced and uninduced conditions.