title: Whole transcriptome sequencing of Reference Jurkat clone E6-1 cells
design: 1x10^6 Jurkat clone E6-1 cells had total RNA extracted using the Zymo Research Quick-RNA miniprep kit (Ngaio Diagnostics) according to the manufacturer's protocol. RNA aliquots from each instance of the Jurkat clone E6-1 cell line were sent to Custom Science (Auckland, New Zealand) for whole transcriptome sequencing. Libraries were prepared using the Illumina Stranded Total RNA prep kit (Illumina), followed by sequencing on a NovaSeq 6000 using a 150 bp paired-end read sequencing design.