title: RNA-seq of Saccharomyces cerevisiae
design: Whole genome DNA of SCDM001, SCDM001_ALE, and SCSA001 was extracted and sequenced using the Illumina NovaSeq and Oxford Nanopore Technologies (ONT) platform at Personalbio (Shanghai, China). For the Illumina paired-end sequencing process, a minimum of 1 g of genomic DNA was utilized for the construction of sequencing libraries for each sample. A library was constructedby Illumina TruSeq Nano DNA LT following Illumina TruSeq DNA Sample Preparation Guide. The library was quality controlled using Agilent High Sensitivity DNA Kit and sequenced using an Illumina NovaSeq platform (Personalbio Co., Ltd) in 2  150bp paired-end mode with a 400bp insert size of the library. High-quality data were obtained from raw data by removing adapter contamination with AdapterRemoval (version 2 and collection with SOAPec (version 2.01) (https://help.rc.ufl.edu/doc/SOAPec).