title: PT_3
design: Transcriptomic mRNA libraries were prepared using the NEB kit and quantified with Qubit and BioAnalyzer, followed by sequencing outsourced to Biokart India Pvt Ltd on an Illumina platform. Quality assessment and processing of reads were performed using Fastqc, Trim Galore, and Trinity for de novo transcriptome assembly, with redundancy removed and validated using cd-hit and RSEM.