title: RNA-seq of adaxial and abaxial rosette leaves
design: A. thaliana [L.] Heynh. ecotype Columbia grown in a greenhouse was used in our study. Double-sided tape was used to separate all the leaves of three to four-week-old seedlings to obtain the adaxial epidermis directly. Then a blade was used to gently scrape off the remaining leaf to obtain the epidermis abaxial side. Total RNA was extracted from the two sides of leaves using the TransZol reagent (TransGen).