title: S1_3-metatranscriptome
design: Metatranscriptome libraries were prepared following TruSeqTM Stranded Total RNA Sample Preparation Kit from Illumina (San Diego, CA), using 5g of total RNA. Shortly, rRNA removal by Ribo-ZeroTM rRNA Removal Kits from Illumina (San Diego, CA), fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illuminas protocol. Libraries were then size selected for cDNA target fragments of 200300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles.All samples were sequenced by the Illumina NovaSeq 6000 platform (150bp*2, Shanghai Biozeron Biotechnology Co., Ltd, Shanghai, China).