title: Whole genome sequencing of Mycobacterium tuberculosis strain isolated in Isreal in 2021
design: The genomic DNA from inactivated bacterial cultures was extracted according to the manufacturers protocol using Maxwell RSC cultured cells DNA kit and Maxwell 16 System (Promega). (https://erj.ersjournals.com/content/erj/52/5/1801163.full.pdf). DNA Paired-end libraries were prepared using the Illumina Nextera XT DNA Library Preparation Kit according to Illumina protocols. For sequencing, we utilized the Illumina MiSeq platform using a 250-bp paired-end read kit v2 or a 300-bp paired-end kit v3.