title: WGS of FSL R5-0773
design: The genomic DNA was extracted by using QIAamp DNA Mini Kit (Qiagen, Valencia, CA) according to the manufacturers instruction, which was modified to include an additional 45 min lysis step. Briefly, cultures grown overnight in BHI broth at 32C were pelleted by centrifugation and subsequently lysed with 20 L of proteinase K (20 mg/mL) by incubation for 45 min at 56C. DNA was eluted twice in 50-L volumes of 10 mM Tris-HCl (pH 8.0), and DNA purity was assessed using a Nanodrop spectrophotometer (Thermo Fischer Scientific, Waltham, MA). Libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego). Samples were pooled and genomic DNA was sequenced in Illumina MiSeq (Illumina, San Diego, CA, USA) with 2  150 bp paired-end reads.