title: Lactobacillus reuteri transcriptome with glycerol in culture medium
design: Total bacterial RNA was extracted using TRIzol reagent (Life Technologies, Grand Island, NY, USA) and purified using an RNeasy Micro Kit (QIAGEN, Germany). First- and second-strand cDNA were synthesized using a superscript double-stranded cDNA synthesis kit (Life Technologies, USA). inally, the cDNAs were sequenced on an Illumina HiSeq 2500 platform.