title: Metagenomics of D. rerio gut microbiome
design: PCR amplification was performed using fusion primers targeting from V3 to V4 regions of the 16S rRNA gene with the extracted DNA. For bacterial amplification, fusion primers of 341F (5-AATGATACGGCGACCACCGAGATCTACAC-XXXXXXXX-TCGTCGGCAGCGTC-AGATGTGTATAAGAGACAG-CCTACGGGNGGCWGCAG-3; underlined sequence indicates the target region primer) and 805R (5- CAAGCAGAAGACGGCATACGAGAT-XXXXXXXX-GTCTCGTGGGCTCGG-AGATGTGTATAAGAGACAG-GACTACHVGGGTATCTAATCC-3) were used. The fusion primers are constructed in the following order which is P5 (P7) graft binding, i5 (i8) index, Nextera consensus, Sequencing adaptor, and Target region sequence.