title: WGS of FSL W5-0628
design: The genomic DNA was extracted by using QIAamp DNA Mini Kit (Qiagen, Valencia, CA) according to the manufacturers instruction, which was modified to include an additional 45 min lysis step. Briefly, single colonies grown on plate count milk agar (PCMA, BCP plate count skim milk agar with 0.2% mass fraction starch (Standards, 2013)) resuspended in Buffer ALT (supplied in QIAamp Mini Kit) were pelleted by centrifugation and ubsequently lysed with 20 L of proteinase K (20 mg/mL) by incubation for 45 min at 56C. DNA was eluted twice in 50-L volumes of 10 mM Tris-HCl (pH 8.0), and DNA purity was assessed using a Nanodrop spectrophotometer (Thermo Fischer Scientific, Waltham, MA). Libraries were prepared using Nextera XT DNA Library Preparation Kit (Illumina, San Diego). Samples were pooled and genomic DNA was sequenced in NextSeq 550 with 150-bp pair-end reads.