CD14+ monocytes were isolated by MACS (Miltenyi Biotech) from the peripheral blood of melanoma patients and healthy individuals. Purity and viability of cells were checked and cells were resuspended in PBS. Library was performed according to manufacturer's instructions (Chromium Next Gem Single Cell 3' reagent V3.1, dual index). Briefly, cells were loaded onto a Chromium Chip.Within each gel bead, mRNA molecules were captured and barcoded during reverse transcription using unique molecular identifiers (UMIs).cDNA was synthesized and amplified, incorporating cell-specific barcodes and UMIs. Amplified cDNA libraries were prepared by adding adapter sequences for downstream sequencing. Excess reagents were removed from the libraries.The quality and concentration of the prepared libraries were assessed using Bioanalyzer.