hippocampus were dissected out from  17-month-old female WT or 3xTg-AD mice treated with or without 400 mGy X-ray irradiation, and total RNA was extracted using Trizol reagent. A total of 100ng of total RNA was used as input RNA for each sample. RNA libraries for RNA-seq were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit following the manufacturer's protocols.