RNA has been isolated from brown adipose tissue using RNeasy Lipid Tissue Mini Kit and eluted with water. 10-15ug of RNA was used for RNA-sequencing. Samples were prepared according to library kit manufacturer's protocol, indexed, pooled, and sequenced on an Illumina NovaSeq 6000.  Basecalls and demultiplexing were performed with Illumina's bcl2fastq software and a custom python demultiplexing program with a maximum of one mismatch in the indexing read.  RNA-seq reads were then aligned to the Ensembl release 101 primary assembly with STAR version 2.7.9a.