Live cells were conjugated to concanavalin A–coated magnetic beads. The antibody was bound to the cell and fragmented with Tn5.Genomic DNA was extracted by ethanol precipitation. To generate libraries, purified genomic DNA was amplified with the barcoded i5 and i7 primer using VAHTS HiFi Amplification Mix. The library PCR products were cleaned up with VAHTS DNA Clean Beads and size selected at 200-500bp in length.