Mice were injected i.p. with αP2X7 HLE Nb-dimer 1 hour and  CD45-PerCP i.v. 3 min before sacrifice, respectively. Kidneys were isolated, minced and leucocytes collected using a Percoll gradient. T cell subpopulations (vascular CD4+, parenchymal NKT CD1dtet+/CD69+, TRM CD1dtet-/CD4+/CD69+ and non TRM CD1dtet-/CD69-) were sorted by FACS and total RNA was extracted using the Qiagen RNeasy Micro Kit. RNA libraries for RNA-seq were prepared using Clonetech RNA with Nextera following manufacturer's protocols.