Total RNA was extracted using the QIAzol Lysis Reagent (79306, QIAGEN) and miRNeasy Micro Kit (217084, QIAGEN) followed by digestion of DNA using the TURBO DNA-free™ Kit (AM1907, Invitrogen). Extracted RNA quality was assessed, and 100 ng of RNA were used for library preparation with NEBNext Poly(A) Isolation Module (E7490, New England Bio Labs) and NEBNext Ultra II RNA Prep Kit for Illumina (E7770, New England Bio Labs)