Tregs were sorted from the BM of control or IR mice.RNA was extracted from sorted cells using a RNeasy Micro Kit (QIAGEN) following the manufacturer's procedure. The RNA amount and purity were quantified using NanoDrop ND-1000 (NanoDrop). Libraries for RNA-seq were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.