Reporter or cell surface marker positive cells were sorted by FACS. Cells loading and library preparation were carried out according to the manufacturer's protocol for the  Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index). Libraries were sequenced using the sequencing format: read 1, 28 cycles; i7 index, 10 cycles; i5 index, 10 cycles; read 2, 90 cycles on Novaseq 6000 system.