Tumors were prepared for single cell suspension with in Liberase (Sigma Aldrich) at 50 mg/mL for 15 minutes at 37°C in RPMI without fetal bovine serum (FBS) and mechanical disruption. Tumor samples were negatively selected to remove CD105+ epithelial cells using CD105 Biotin (eBioscience) and Pierce Streptavidin Beads (ThermoFisher). Libraries were constructed for SMART-seq RNA following manufacturer's instructions