For early time points (4, 6, and 8 hpf), cells were dissociated with the addition of 200 µL of pre-warmed FACSmax cell dissociation solution (Genlantis T200100) for 5 minutes resuspending gently up and down at RT. For later time points (10, 12 and 24 hpf) cells were dissociated with the addition of 200 µL of pre-warmed Protease solution (100 µL Trypsin-EDTA (0.5%), no phenol red, Thermofisher 15400054, 20 µL 10X PBS0, 80 µL H2O) for 6 minutes on a shaker (Eppendorf Thermomixer Comfort) at 28°C and 400 rpm, resuspending vigorously every 2 minutes. The detailed, step-by-step scT-ChIC protocol is available at: https://www.protocols.io/private/714FFF81809D11EE958A0A58A9FEAC02