DNA was extracted using a Qiagen Dneasy kit with an additional lysozyme treatment prior to lysis. Libraries were prepared using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina and a Nextera® XT Index Kit v2 Set A.  Libraries were sequenced on a NextSeq P1 flow cell (150 PE) with ~2.1 million reads per sample (~300 reads/mutant).