Nuclear isolation by hypotonic buffer and mechanical lysis followed by centrifugation in 0.3M sucrose buffer. CUT&Tag was then performed following the protocol from Kaya-Okur et al. (https://doi.org/10.1038/s41596-020-0373-x). IDT primers (Illumina, 20027213) and PCR enzyme mix (NEB, M0541S) were used for library preparation, and AMPure beads (Beckman Coulter, A63881) were used for PCR cleanup CUT&Tag with DNA-sequencing