RNA was harvested using Magzol reagent (Magen, R480102). 1 ug of total RNA used for the construction of sequencing libraries. RNA-Seq (Quantification) uses a new generation of high-throughput sequencing technology to study the gene expression pattern of eukaryotic cells and provide accurate digital expression profile detection by sequencing and comparing transcripts. The experimental procedure includes: 1. sample QC, 2. mRNA isolation, 3. mRNA fragmentation, 4. cDNA synthesis, 5. end repair,add A and adaptor ligation, 6. PCR, 7. library QC.