MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit and GLOBINclear (Thermo Fisher Scientific, Bothell, WA) to deplete alpha and beta globin mRNA from whole blood samples according to the package inserts. DNase treatment was incorporated into both BAL and blood RNA extractions to reduce contamination by genomic DNA. The TruSeq RNA Exome kit was used to prepare cDNA libraries.