Cells were lysed in TRIzol LS reagent (Thermo Fisher Scientific) then RNA was purified and DNase treated using a Direct-zol RNA Miniprep kit (Zymo Research). Novel RNA-Seq pipeline. Index is added during initial pA priming and pooled samples amplified using template switching oligo. P5 is added by tagmentation by Nextera transposase and PCR. Sequencing was performed on NextSeq2000 (Illumina) following Illumina protocol 1000000109376 v3.