Cells were harvested and RNA was isolated with Trizol. Depletion of ribosomal RNA. Exonuclease digest and negative selection of poly(A) transcripts to enrich for circular RNAs. cDNA library preparation (SMARTer) and PCR amplification, according to Fuchs et al. PMID 36070299 Oxford Nanopore long-read RNA sequencing (SQK-LSK109 kit with native barcoding kit EXP-NBD104, BC01-04)