Total RNA (1µg/sample) was used as input for the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB) After rRNA depletion, libraries were made using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina®, and quantified using KAPA Library Quantification Kit. Libraries were pooled and sequenced on the NextSeq 500/550.