Subcellular fractionation and isolation of chromatin bound nascent RNAs was adapted from Nesterova et al 2019. Briefly, cell nuclei were isolated in lysis buffer with a sucrose cushion. Nuclei were then lysed in a urea buffer, and the insoluble pellet containing the chromatin fraction and associated nascent RNAs was purified using the Zymo Direct-zol RNA Miniprep Kit (R2051). Library prep was performed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (E7760S) using the rRNA depletion (E6310L) workflow