Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sheared using Bioruptor® Pico sonication device (Diagenode Cat# B01060001) ChIP-seq libraries were prepared using IP-Star® Compact Automated System (Diagenode Cat# B03000002) from input and ChIP'd DNA using MicroPlex Library Preparation Kit v3 /96 rxns (Diagenode Cat# C05010002) with 24 UDI for MicroPlex v3 - Set I (Diagenode Cat# C05010008). 94 ng of DNA was used for ip samples. Chromatin corresponsing to 1% was set as an input. Optimal library amplification was assessed by qPCR using KAPA SYBR® FAST (Sigma-Aldrich) on Light Cycler® 96 System (Roche) and by using High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent). Libraries were then purified using Agencourt® AMPure® XP (Beckman Coulter) and quantified using Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854). Finally, their fragment size was analyzed by High Sensitivity NGS Fragment Analysis Kit (DNF-474) on a Fragment Analyzer™ (Agilent).