RNA was extracted from dissected proximal and distal limb buds from 2 independent pools of wild type limb buds (n=2) and from two independent pools of mutant limb buds (n=2). The extraction was performed using the RNAeasy Plus mini kit. Next, mRNA enrichment, library preparation and flow-cell preparation for sequencing were performed by the IRCM Molecular Biology Core Facility according to Illumina's recommendations. Sequencing was done on a Nextseq500 instrument with a paired-end 50 cycles protocol. Library and flow cells were prepared by the IRCM Molecular Biology Core Facility according to Illumina's recommendations and sequenced on Illumina in a 50 cycles paired-end configuration.