Whole skins from WT and Up_del mice with and without alopecia female adult mice were harvested after shaving with an electric clipper. The hypodermal layers were removed by scraping from the dermis with forceps. The rest of the skin containing dermis and epidermis was minced with scissors and digested for 2 hrs at 37°C in RPMI containing 0.25mg/mL of Liberase T-Flex and 1ug/mL of DNAse I followed by the addition of 0.25 % Trypsin-1mM EDTA for 10 min. Cells were isolated and immunostained with anti-CD45 and Sytox-Green (SG) before being FACS sorted as live CD45+ and CD45-. Sorted live CD45+ and CD45- cell counts were adjusted and captured as per 10X genomics protocol using 5'-HTv2 kit for single cell RNA sequencing. Library construction was performed in accordance with 10X Genomics protocol (Chromium Next GEM Single Cell 5' HT Gene expression (GEX) library protocol, CGOOO423, Rev C).