Cells were removed from plates with TrypLE, counted, and diluted to 1000cells/ul in DPBS+1% FBS. RNA was extracted from cells and sgRNAs were barcode captured following manufacturer's instructions as part of 10X Genomics sequencing. Library was constructed according to the manufacturer's instructions (10X Genomics scRNA-seq, GEX libraries) and Feature Barcoded CRIPSR Capture (CRISPR Capture libraries) at the CHOP Center for Applied Genomics.