ICM or TE was dissected from diapause embryo and lysed in 6uL of 1x direct lysis buffer(TaKaRa, #635013) including RNase Inhibitor(TaKaRa,#2313A). 3uL of lysate was used for reverse transcription. Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.