Parasites were saponin extracted and stored in trizol. Total RNA was extracted by phenol-chloroform and treated with Dnase, then polyA mRNA was extracted using NEBNext® Poly(A) mRNA Magnetic Isolation Module. First strand cDNA synthesis was performed using Sensiscript Vand Ampure bead cleanup. Second strand synthesis using NEBNext® Ultra™ II Non-Directional RNA Second Strand Synthesis Module was performed and another ampure bead cleanup. Samples were covaris sheared and libraries prepared using Kapa Hyper prep kit