RNA extraction from hypocotyls for RNA sequencing was performed with the RNeasy Mini Kit (QIAGEN) and treated with DNase Turbo DNA-free kit (Thermo Fisher Scientific). 50 to 60 hypocotyls were pooled per biological replicate. RNA quality was assessed on a 2100 Bioanalyzer (Agilent Technologies) polyA isolation with TruSeq directional library preparation