Cells were harvested and RNA was isolated with Trizol. Depletion of ribosomal RNA was performed by enzymatic digestion. The ribodepletion was validated by qRT-PCR and a depletion of 95-99% was regularly reached. The ribodepleted RNA was used for generation of total RNA sequencing libraries with the TrueSeq Stranded mRNA kit (Illumina San Diego, USA) according to the manufacturer. The final libraries were stored at -20°C until sequencing. Libraries were sequenced on a HiSeq4000 (Illumina, San Diego, USA) with a paired-end read length of 2 × 150 nucleotides. On average 100 Mio read pairs were generated per sample.