CD8+ OT-I T cells were sorted from the dual-color transfer system at day 7 post adoptive transfer from B16-OVA tumor and loaded onto  the Chromium Controller according to their respective cell counts to target 9000 single cell GEMs (gel beads in emulsion) per sample. Libraries were prepared using the Chromium Single Cell 3' v3 Library and Gel Bead Kit (10x Genomics)