Ribosome profiling (Rnase I footprinting, size selection, RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification) was done as described in "Reprogramming of translation in yeast cells impaired for ribosome recycling favors short, efficiently translated mRNAs" Gaikwad, Ghobakhlou, et al. eLife 2021;10:e64