Cell lysis and separation of chromatin fractions were performed using the standard protocol Svejstrup et al., (2003), except that we used ammonium sulfate to 0.5 M final concentration instead of 1 M to release chromatin-associated proteins, as we have described previously DNA fragments were then cleaned using the ChIP DNA clean & concentratorTM kit (ZYMO RESEARCH). Aliquots of 1 to 2 ng of DNA from each sample were tested for proper shearing using a fragment analyzer (Agilent 2100 Bioanalyzer G2938C), and then sent for library preparation using the SMARTer ThruPLEX kit and subsequent pair-ended sequencing using Illumina NovaSeq S4-200 cycle flow cells in individual lanes.