Single cell suspension was prepared and fluorescence activated cell sorting (FACS) was performed to separate tdT+ and tdT- cell. Sorted cells from two animals were pool together into one sample. Duplicated tdT+ and tdT- samples of each genotype were subjected to scRNA-seq using the Chromium Single Cell 3' Library and Gel Bead Kit (v3.1 10x Genomics, Pleasanton, CA) and the Chromium X.