RNA extraction was performed with Maxwell RSC 48 with simplyRNA Cells Kit (Cat No: AS1390, Promega). Iodoacetamide (Cat No: I6125-10G, Sigma Aldrich) was dissolved in absolute ethanol with 6mM final concentration. 500ng RNAs were used for library preparation. Libraries were prepared with Lexogen's QuantSeq 3′ mRNA-Seq V2 FWD with UDI for Illumina (Cat. No: 193.384, Lexogen) using 500ng of input RNA for all samples with 12 cycles. All steps were performed according to the manufacturer's protocol.