Total RNA and µRNA was obtained from the aortic arch using the miRNeasy Mini Kit (Qiagen). Library preparation, sequencing and primary data analysis were performed at GenomeScan B.V. (Genomescan B.V., Leiden, The Netherlands). The NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used to process the samples. The sample preparation was performed according to the protocol "NEBNext Ultra Directional RNA Library Prep Kit for Illumina"(NEB #E7420S/L)