the C. hirsuta genomic DNA was extracted from rosette leaves of 4-week-old plants by Qiagen DNeasy Plant Maxi Kit (Cat No. 68163). For DNA library preparation, about 5 μg genomic DNA was first sheared by a Bioruptor® Pico sonicator (Diagenode) for 6 cycles (30 sec on/30 sec off) at 4°C to 200-400 bp fragments, followed by end repair, A-tail reaction, and adaptor ligation processes.