Brain tissue was extracted, and meninges were removed. Left hemispheres were frozen in optimal cutting temperature (OCT) compound and sectioned for spatial transcriptomics. Brain tissue was sectioned coronally (10 um thickness; bregma -2.0 to -2.2) and slices were placed on a 10X Genomics Visium Spatial Gene Expression Slide. Spatial trancriptomice sequenccing libraries were prepared with 10x Genomic Visium Gene Expression slides to manufacturer's instructions