Total RNA of Hoechst-high or Hoechst-low cells isolated by FACS was extracted using RNeasy micro (Qiagen). 1 ng of the total RNA was reverse transcribed and amplified for 11 cycles using SMART-Seq HT (Clontech Laboratories, Z4456N). The amplified cDNA was used for preparation of sequencing libraries using the Nextera XT DNA Library Preparation Kit (Illumina, FC-131).