Total RNA was extracted from bulk ACC tissue with the QIAGEN RNeasy Mini Kit RNA libraries for RNA-seq were prepared using the SMARTSeq v4 Ultra Low Input RNA kit for Sequencing (PN 091817, Takara Bio Europe, Saint-Germain-en-Laye, France) following manufacturer's protocols. Full length cDNAs were generated from 5ng of total RNA, with 10 cycles of PCR for cDNA amplification by Seq-Amp polymerase.