The cells were treated using CST SimpleChip enzymatic kit with following modifications. Four 150 mm dished of cells were crosslinked first by EGS 5mM, for 20 min followed by 1% formaldehyde for 10 min and washed with PBS. From here cells were treated with MNAse and sonicated  according to the kit instruction. We used 6ul of magnetic beads to pre-clear the chromatin and 0.18mkg of antibody to precipitate chromatin. In addition, we added the last washing step with 0.15% Sarcosin in 10mM Tris 1mM EDTA.  Experiments were conducted using β-catenin antibody D10A8 (6 μg, Cell Signaling Technology).