PBMCs were isolated from human whole blood, cryopreserved, and 4 control and 4 hypertensive samples were thawed for use. Samples were then stained with hashtags and extracellular antibodies. Finally, samples were washed and experimental groups pooled to run on the 10X Genomics controller. Libraries were prepared according to manufacturer's instructions for 10X Chromium Single Cell 5' Reagent Kit v2 with the 5' Feature Barcode Library Kit by the Vanderbilt VANTAGE core with sequencing on a NovaSeq 6000 targeting 50,000 reads per cell