PBMCs were isolated from heparin blood of four mice using standard Ficoll gradient. Cells 513 were counted in a hemocytometer using Trypan blue for live/dead staining. Viability was >95% for all samples. Single-cell transcriptome analysis was performed using Chromium Next GEM Single Cell 3' Kit v3.1 (10xGenomics) with 5,000 cells input after the manufacturer's protocol. Generated single-cell libraries were sequenced by Novogene on an Illumina NovaSeq using PE150 sequencing.