Total RNA extraction was performed using the RNeasy Mini Kit (Qiagen, #74104), according to the manufacturer's instructions. Genomic DNA was then eliminated with QIAshredder (Qiagen, #79656) followed by on-column DNAse digestion. 500ng of total RNA was used as input for library prep. RNA-seq libraries were prepared using the TruSeq Stranded mRNA kit (Illumina, #20020594).