To delineate the single-cell dynamics of PBMCs during latent and reactivated HCMV infection, we procured PBMCs from the peripheral blood of humanized mice. These were categorized into two groups: two wild-type (Mock), and two HCMV-infected (HCMV). Initially, NHDF cells harboring HCMV were introduced into the peritoneal cavity of NOD-scid IL2Rgcnull mice. Following a 4-week period peripheral blood was harvested and partitioned from each mouse group RNA libraries were prepared for sequencing using standard Illunina protocols