Total RNA was extracted from CD4+ and CD8+ sorted TIL with the Qiagen RNEasy Mini Kit. 100ng of RNA was used to generate cDNA and a strand-specific library following the manufacturer's protocol (NuGEN Technologies, Inc., San Carlos, CA). These libraries were then sequenced on the Illumina NextSeq 500 v2 sequencer with a 75-base paired-end run in order to generate 30-40 million read pairs per sample.