Total RNA was isolated from sorted E14.5 fetal liver erythroblast stage 1 using miRNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. In order to construct cDNA libraries with the TruSeq Stranded mRNA Sample Prep kit (Illumina, USA), 1 mg of total RNA was used. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100 nt paired-end sequencing by Illumina NovaSeq (Illumina, USA).