Sensory ganglia were dissociated in 40 units papain, 4 mg/ml collagenase, 10 mg/ml BSA, 1 mg/ml hyaluronidase, 0.6 mg/ml DNAse in DMEM:F12 + 1% pen/strep + 12.5 mM glucose for 25 min at 37C. Digestion was quenched using 20 mg/ml ovomucoid (trypsin inhibitor), 20 mg/ml BSA in DMEM:F12  + 1% pen/strep + 12.5 mM glucose. Ganglia were gently triturated with fire-polished glass pipettes. Neurons were then passed through a 70-micron filter to remove cell doublets and debris. Neurons were pelleted and washed 4x in 20 mg/ml ovomucoid (trypsin inhibitor) , 20 mg/ml BSA in DMEM:F12 + 1% pen/strep + 12.5 mM glucose followed by 2x washes with DMEM:F12 + 1% pen/strep + 12.5 mM glucose all at 4C. After washing, cells were resuspended in 45 microliters of DMEM:F12 + 1% pen/strep + 12.5 mM glucose. library preparation were performed according to manufacturer's instructions (10x genomics chromium single cell kit, v3)