Whole blood was collected by cardiac puncture. Red blood cells where lysed with ACK buffer and removed by density centrifugation in a 7.5% BSA cushion (300g for 5min).  Cells were strained with a 40um sieve.     For kidney cells isolation, animals were perfused with PBS-5mM EDTA through the left ventricle, then kidneys where collected and  minced into pieces with a razor blade. Tissue fragments were digested in HBBS – 0.15mg/ml Liberase TM for 25min at 37deg C with vigorous agitation. Digestion was stopped with FBS. The digestion mix was passed through a 70um sieve and spun down for 5min at 300g. The pellet was spun down on a 7.5% BSA cushion, for 4min at 250g, twice, to clear debris.     For heart, non-myocyte cells, hearts were perfused by the Langendorff method and digested with 0.15mg/ml Liberase TM. The LV of sham-operated animals or the infarcted area of the LV of infarcted hearts was cut and triturated with a plastic Pasteur pipette. Digestion was stopped with FBS. Myocytes were separated by centrifugation at 50g for 5 mins. The supernatant with the rest of the cells was pelleted at 300g for 5 mins. Cells where then passed through a 70um strainer.    For liver cells, livers were perfused and then digested in Liver Digest Medium. Livers were dissected and hepatocytes were separated by centrifugation at 30g for 4minutes. The rest of the cells (supernatant) were pelleted by centrifugating 5min at 300g. Non-hepatocyte liver cells were passed through a 70um sieve. Hepatocytes and the rest of the liver cells were combined 1:1.    Pancreatic islets were isolated by perfusion of the pancreases with CiZyme through the common hepatic duct. Pancreases were removed and digested at 37 °C for 17 min. After two washes with RPMI medium with 3% FBS, islets were separated into a gradient using RPMI medium and Histopaque (Sigma-Aldrich). Islets were then hand-picked and rendered single by digesting for 10min at 37deg C in Accutase. Standard library construction protocol using 10x Genomics Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3, and Chromium Single Cell B Chip Kit.