1 µg total RNA was employed for cDNA library construction according to Oxford Nanopore Technology (ONT) protocol. Full length mRNAs were reverse transcripted by SuperScript IV First-Strand Synthesis System (Invitrogen) and amplified for 14 circles with LongAmp Tag (NEB). Then PCR products were under FFPE DNA repair and end-repair (NEB) steps and adaptor ligation using T4 DNA ligase (NEB). DNA purification was performed by Agencourt XP beads according to ONT protocol. Long-read nanopore sequencing