Wild-type, aktmutant, Tg(kdrl:mCherry) tissue  were dissected at 24 hpf. Dissected  tissues were dissociated into single cell suspensions and subjected to FACS.mCherry+ cells, which had 85% cell viability, were loaded onto the 10X Genomics Chromium instrument for a targeted recovery of 10,000 cells per sample. 10X Genomics Chromium Next GEM Single Cell 3' Library Construction Kit V3.1 (CG000204) was used to generate libraires according to manufacturer intructions 10X Genomics Chromium Next GEM Single Cell 3' Library Construction Kit V3.1 (CG000204) was used to generate libraires according to manufacturer intructions Transcriptomic single cell, 10x genomics