Protein-DNA interactions were mapped using the CUT&Tag method, employing H3k27ac/specific antibody to target [protein/histone of interest] in the samples The specific antibody (anti-H3K27ac) binds in situ to the chromatin-associated protein and is subsequently anchored to the A-Tn5 transposition fusion protein. Upon activation of the Tn5 transposable enzyme, it fragments the chromatin selectively bound to the target protein, efficiently generating high-resolution, low-background fragment libraries.