Total RNA was extracted using RNeasy Plus Mini Kit (QIAGEN, # 74134). RNA libraries were prepared using Ampliseq Library Prep Kit following manufacturer's protocols for targeted amplicon amplification Other: Mouse 46K, Ion AmpliSeq Panels Mouse 46K genes for transcriptome analysis of PBM and PBM-R tumors; Mouse transcriptome,  Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit for transcriptome analysis of myeloid cells.