FACS sorted cells were stained with DAPI for dead cell exclusion. The cell suspensions were counted with Moxi cell counter and diluted according to manufacturer's protocol to obtain 5000-10000 single cell data points per sample. Each sample was run separately on a lane in Chromium controller with Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10xGenomics). Library preparation was conducted according to the manufacturer indications.