Cells were fixed, lysed, and sonicated using the CST Simple CHIP Plus Kit. 10ug of DNA was incubated with 4ug of each antibody (BRD4, Pol II, H3K27ac) over night, and then incubated with the protein G beads for 2 hours. After washing and elution, DNA was isolated with kit provided in the CST Simple CHIP Plus kit. NEB NextUltra DNA Library Preparation kit