Male mice homozygous for Zfp92 mutation and wild-type littermates were used for islet isolations at 14 weeks of age.  Mouse islets were isolated following injection of 0.6 mg/mL Collagenase P (Roche) into the pancreatic bile duct. Partially dissociated tissue was fractionated using a Histopaque-1077 (Sigma) gradient followed by hand-picking of islets.  Islets from individual mice were used for separate RNA sample isolations. All mice used for experiments were on C57Black/6J background.  Wild type and Zfp92 knockout islets were used in the experiment. Total RNA was extracted from isolated mouse pancreatic islets using the Maxwell simplyRNA kit (Promega). cDNA libraries were constructed using  Illumina library prep kit at Novogene.