Livers were removed, preserved in RNALater for at least 24 hours, and then RNA was harvested using the RNeasy Plus Mini Kit (QIAGEN, Germantown, MD) following the manufacturer's instructions. RNA quantity and purity were quantified by NanoDrop1000 (Thermo Fisher Scientific, Waltham, MA). 1μg of total RNA was used for the construction of sequencing libraries. NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) was used to create the sequencing libraries.