Single cell suspensions of microdissected embryonic hindbrain tissue was performed using the Papain Dissociation System (Worthington). Isl1mn-GFPon cells were isolated using FACS, with the gates opened to GFP-negative cells at the conclusion of the sort to a total cell number of approximately 10,000-12,000 10x genomic Chromium Next GEM Single Cell 3' GEM-Library construction