We extracted RNA from NSCs and performed next-generation sequencing, using RNA ScreenTape kit (catalog #5067-5576; Agilent Technologies), D1000 ScreenTape kit (catalog #5067-5582; Agilent Technologies), Qubit RNA HS Assay kit (catalog # Q32852; Invitrogen), and Qubit DNA HS Assay kit (catalog #32854; Invitrogen) were used for each specific step for quality control and quantity determination of RNA and library preparation For mRNA library preparation: TruSeq RNA Library Prep Kit v2 was used (Illumina). In brief, 1 µg was used for the library construction; library was eluted in 20 µL of elution buffer. Libraries were adjusted to 10 mM, and then 10 µL (50%) from each sample was collected and pooled in one tube