Formalin-fixed, paraffin-embedded samples were processed according to the Visium CytAssist Spatial Gene Expression for FFPE – Deparaffinization, H&E Staining, Imaging & Decrosslinking protocol (CG000520 Rev B, 10x Genomics) Libraries were constructed according to the Visium CytAssist Spatial Gene Expression Reagent Kits User Guide (CG000495, Rev E, 10x Genomics)