MuSCs were then trypsinized (0.05% trypsin 0.53M EDTA, Mediatech, Manassas VA) to create a single cell suspension. Cells were then counted on an automated cell counter and RNA library prep was done by the ASU Genomics Core facility. Samples were processed using a 10X Chromium single cell 3' GEM, Library and Gel Bead kit V3 with a Chromium single cell B chip. The quality of each library was determined using Agilent TapeStation automated electrophoresis.