Briefly, cells were harvested, counted, and centrifuged for 3 min at 1,000 rpm at room temperature. About 10,000 cells were washed using washing buffer followed by incubation with Concanavalin A beads at room temperature for 10 min. Next, 1 μl primary antibody was incubated with cells on a rotating platform at 4°C overnight. Secondary antibody incubation followed by pA/G-Tn5 incubation were performed on a rotating platform at room temperature each for 1 h. Between each of the above steps, the beads were washed using corresponding buffer. To activate the activity of Tn5, the beads were incubated in Tagmentation buffer at 37°C for 1 h followed by stop tagmentation using EDTA and extract DNA using DNA clean beads. Cut&Tag libraries preparation using Hyperactive universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903)