Cells were collected by trypsinization and suspensions prepared according to guidelines from 10X genomics.  Suspensions were adjusted to a concentration of 1000 cells per microliter and loaded to one capture well to obtain 10,000 cells. Construction of the library was perfomed according to manufacturers instructions.  10X genomics libfraries were prepared using v3 chemistry Libraries were sequenced to an average of 23000 reads per cell quantifying expression for a median of 1509 and 2733 transcripts per cell for palbociclib treated and control, respectively