Very young buds (squares) from individual three plants of control and RNA knock-down line were collected at the very early stage of flowering. Plants were grown in experiment field of North Farm Experiment Station at Mississippi State, MS (33.4° N, 88.8°W) in summer 2017. Standard watering and irrigation. Total RNAs from samples were extracted by using the modified hot borate method combined with DNase I digestion and final cleanup with RNeasy Mini Kit. The quality of each RNA sample was validated with 1% agarose gel electrophoresis and Bioanalyzer 2100 system using RNA 6000 Nano Kit and  and the concentration was quantitated by the Nanodrop ND-1000 Spectrophotometer and Qubit flourometer using Qubit RNA BR Assay Kit. RNA-Seq libraries were created using the TruSeq Stranded mRNA Library Prep Kit at the Institute for Genomics, Biocomputing and Biotechnology (IGBB), Mississippi State University, MS 39762. As for the multiplexing purpose, all 6 stranded RNA-Seq libraries were independently ligated with 6 TruSeq RNA adaptors barcoding 6 compatible indexes. The quality of libraries were validated with Bioanalyzer 2100 system using DNA 1000 Kit o analyze the library size and the adaptor contamination. The library concentration was quantified with Qubit flourometer using Qubit dsDNA HS Assay Kit and qPCR assays using KAPA Library Quantification Kit. All six libraries were diluted into a concentration of 20 nM based on the concentration from qPCR analysis, and further pooled together with equal volume from each library into one pooled sample.