Total RNA was extracted using the Qiagen RNeasy kit (QIAGEN), and during the purification process of the sample, DNA enzyme (QIAGEN) was used for on-column digestion of the DNA. RNA quality and concentration were measured using an Agilent 2100 biological analyzer. Ribosomal RNA molecules (rRNA) were eliminated using Ribo-Zero rRNA removal kits (Illumina). RNA sequencing libraries were constructed using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturer's instructions, and purified library products were evaluated using 2200 Tapestation (Agilent Technologies) and Qubit®2.0 (Thermo Fisher Scientific). Finally, single-stranded DNA molecules were clustered and sequenced for 150 cycles on an Illumina HiSeq 3000 system (Illumina).