Nuclei were extracted from cells and treated with transposition mixture containing Nextera Tn5 Transposase for 30 minutes at 37oC with 1000 RPM mixing. Transposed fragments were then purified using Qiagen MinElute columns, PCR amplified, and libraries were purified with Agencourt AMPure XP magnetic beads with a sample to bead ratio of 1:1.2. Samples were sequenced on an Illumina NovaSeq with paired-end 50bp reads with 100 million reads per sample.