Cell and EV total RNA were extracted by Trizol® (Invitrogen) and purified by RNeasy® Mini Kit (Qiagen). Briefly, cell or EV pellets 700 µl Trizol®. Subsequently, 140 µl of chloroform was used for phase separation. Trizol® and chloroform mix were centrifuged at 12,000 x g for 15 mins for phase separation. 300 µl RNA-containing top clear phase were transferred to a fresh tube and 400 µl absolute ethanol was added and mixed. 700 µl of sample were transferred to RNeasy spin column. DNAse treatment was performed after first 350 µl RW1 washing step. Finally, RNA was eluted in 30 µl RNase-free water. RNA concentration was assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The RNA yield and size distribution were analyzed using an Agilent 2200 Tapestation with high sensitivity RNA Screentape (Agilent Technologies, Foster City, CA, USA). For small RNA library preparation, RNA aliquots were used for library preparation using NEBNext Multiplex Small RNA library preparation kit (New England Biolabs, Ipswich, MA, USA). The PCR amplified cDNA construct (from 140–160 bp) was purified using a QIAquick PCR Purification kit (Qiagen). The purified cDNA was directly sequenced using an Illumina MiSeq 2000 platform (Illumina, San Diego, CA, USA).  For long non coding RNA library preparation, libraries were constructed using Ribo-Zero Magnetic Gold Kit (Human) (Illumina, San Diego, CA, USA) and NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. Libraries were tested for quality and quantified using qPCR (Kapa Biosystems, Woburn, MA, USA). The resulting libraries were sequenced on a HiSeq 2500 instrument that generated paired-end reads of 100 nucleotides.