Following auxin-mediated Saft protein depletion for the specified durations, 2 million cells were harvested per sample by centrifugation at 300 g for 5 min and washed once with PBS. Total RNA was isolated and DNase I treated after cell lysis with the RNA isolation kit provided by the VBC core facilities, which uses a lysis step based on guanidine thiocyanate (adapted from (Boom et al., 1990)) and SpeedBeads magnetic carboxylate modified particles (Merck) and eluted in 50ul water. cDNA libraries from 500ng total RNA were prepared with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) following the manufacturer's protocol.