8 day old sorghum roots were cut in a laminar flow cabin, pooled and its RNA extracted using MAXWELL 16 LEV system by PROMEGA with the Plant RNA Kit. Total RNA was extracted, frozen, and sent to sequencing. Stranded libraries were prepared using TrueSeq Stranded mRNA KIT (Ilumina). Paired-end sequencing (2x100bp) was performed using NovaSeq6000 (Ilumina) at minimum depth of 45M.