Genomic DNA was extracted from 1 g of ground young cv. 'Syrah' leaves and the Illumina libraries were prepared as previously described (Galli et al., 2018). The VviNAC61 sequence was transferred from the pENTR/D-TOPO to the Gateway-compatible destination vector pIX-HALO (Bartlett et al., 2017). The HALO-VviNAC61 and GST-HALO (used as negative control) fusion proteins were in vitro translated using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries. The DAP-seq was performed according to previously described procedure (Galli et al., 2018) The eluted DNA fragments are PCR amplified with a full-length indexed TruSeq primer and directly sequenced on the Illumina NextSeq 500.