The RNA extraction was done using TRIzol reagent (Invitrogen, 15596026) following the manufacturer's protocol from BMDMs stimulated by indicated stimulations for 9 h. RNA was quantified using the Quant-iT RiboGreen RNA assay (Thermo Fisher Scientific) and quality checked by the 2100 Bioanalyzer RNA 6000 Nano assay (Agilent) or 4200 TapeStation High Sensitivity RNA ScreenTape assay (Agilent) prior to library generation.  Libraries were prepared from total RNA with the TruSeq Stranded mRNA Library Prep Kit according to the manufacturer's instructions (Illumina PN 20020595). Libraries were analyzed for insert size distribution using the 2100 BioAnalyzer High Sensitivity kit (Agilent), 4200 TapeStation D1000 ScreenTape assay (Agilent), or 5300 Fragment Analyzer NGS fragment kit (Agilent). Libraries were quantified using the Quant-iT PicoGreen dsDNA assay (Thermo Fisher Scientific ) or by low pass sequencing with a MiSeq nano kit (Illumina).