RNA was extracted using TRIzol Reagent along with Pure Link RNA Mini Kit (Invitrogen) according to manufacturer instructions and assessed for quality control parameters of minimum concentration and fidelity (i.e. 18S and 28S bands, RIS >8). Libraries were developed using the Illumina TruSeq mRNA Stranded Library Prep Kit (Set-A-indexes), quantified with a Qubit fluorimeter (Invitrogen), and assessed for quality and size using the QIAxcel Advanced System Samples were pooled into a single library (n=17 pooled samples per library) and sequenced using the NextSeq 500 High Output Kit (150 cycles, paired end 75bp) on the  Illumina NextSeq 500 platform.