Primary cells from lung were digested using an enzyme cocktail (CollageaseII/DispaseII /DNAseI) and subjected lung cell suspension to CD45-based MACS to leukocytes and non-leukocytes. Cells were multiplexed from three different animals in each group and the proportion of CD45+ cells were reduced to 50% to avoid oversampling. Several washing steps were performed before performing GEM production. Barcoding and library preparation was done using Chromium single cell 3' Kit (v2, 10x Genomics).