iAssembloids were dissociated with dounce homogenization in a sucrose based homogenization buffer and filtered through a cell filter. The suspension were then mixed with optiprep (final concentration 25%) and layered ontop of a 30% and 40% optiprep layer). The columns were spun down at 10000g for 18 min at 4C. The nuclear layer was extracted (layer between 30% and 40% optiprep and snRNAseq was performed 10X Chromium v3, Fluent Biosciences PIP-seq T2