Isolated microglia were sent to Shanghai Megiddo Biological Pharmaceutical Co., Ltd. for RNA sequencing. Total RNA from flow-sorted microglia was extracted using TRIzol® Reagent according to the manufacturer's instructions (Invitrogen) and genomic DNA was removed using DNase I (TaKara). RNA quality was assessed by 2100 Bioanalyser (Agilent Technologies) and quantified by ND-2000 (NanoDrop Technologies). The library was then prepared following Clontech-SMART-Seq™ v4 Ultra™ Low Input RNA kit for sequencing using 10ng of total RNA. Paired-end RNA sequencing was performed on Illumina NovaSeq 6000 sequencer (2 × 150bp read length).