Brewer thioglycolate medium (3%, 4 mL) was injected intraperitoneally into mice to recruit macrophages and neutrophils to the peritoneal cavity. Mice were sacrificed either 1 day or 4 days later and peritoneal exudates cells were harvested by lavage with PBS.  1 µg/mL LPS (Escherichia coli O111:B4) and 15 µg/mL VISTA-COMP for 2 hours. RNA content was isolated from the conditioned macrophages or neutrophils using the RNeasy Mini Kit. TruSeq Stranded mRNA Library Prep Kit.