The cells were rapidly washed two times in ice-cold PBS, and total RNA was isolated following the Direct-zol RNA Microprep Kit (ZYMO RESEARCH) manufacturer's manual. The library kit was the CORALL RNA-Seq V2 Library Prep Kit with UDI 12 nt. rRNA depletion was done with RiboCop rRNA Depletion Kit for Human/Mouse/Rat (HMR) V2