Total RNA was isolated from N2A cells using the TRIzol reagent (Invitrogen) according to the manufacturer's protocol. For the rRNA-depleted RNA-seq library, sample quality was assessed by Bioanalyzer 2100 Eukaryote Total RNA Pico (Agilent Technologies, CA, USA) and samples were quantified by Qubit RNA HS assay (ThermoFisher #Q32852). Ribosomal RNA depletion was performed with Ribo-zero rRNA Removal Kit (Illumina Inc., San Diego, CA) followed by NEBNext® UltraTM II Nondirectional RNA Library Prep Kit for Illumina® per manufacturer's recommendation.