Mice were euthanized and collected kidneys in their week of 12 weeks of age. Mouse kidney outer cortex tissues were dissected and homogenized in QIAzol. Total RNA samples were extracted by RNeasy Plus Universal Kit (QIAGEN, Hilden, Germany) following manufacture's protocol including removal of genomic DNA step. RNA libraries were prepared for sequencing using standard Illumina TruSeq Stranded mRNA library protocol.