RamDA-seq was performed using 100 cells. Briefly, hematopoietic stem cells (100 cells) were directly sorted into the cell lysis buffer (10 U RNasein plus, 10% Roche Cell Lysis Buffer, 0.3% NP40, and RNase-free water) in a 96-well PCR plate.The cell lysates were then denatured and treated with genomic DNA digestion mix containing DNaseI. Firrst strand  cDNA was then synthesized with first not-so randam primers using PrimeScript RT reagent kit (Takara Bio), followed by second strand dDNA synthesis with second not-so random primers using Klenow Fragment (New England Biolabs). Sequencing libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina) according to the manufacturer's protocol.