Total RNA was harvested using PureLink RNA Mini Kit (Invitrogen). 800 ng of total RNA was used to generate the library. The kits used for the library prep were NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) as described in the manufacturer's manual.