RNA was isolated from frozen liver tissue from the left lobe using Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). To ensure complete DNA removal, which is necessary for the downstream RNA sequencing, we used an RNase-Free DNase kit (Qiagen, Germany). RNA concentrations and purity were determined by NanoDrop 1000 Spectrophotometer (Thermo Fisher, USA). In total, nine RNA samples extracted from the left lobe section of the livers of the STAM, STAM + ATGLi, and noSTZ + NC mice were sent to Lexogen at the Vienna Biocentre Core Facility (Vienna, Austria) for library preparation using the Lexogen's QuanSeq 3' mRNA-Seq kit to generate Illumina-compactible libraries from polyadenylated RNA. The prepared libraries were then sequenced on the Illumina HiSeq 4000 (Illumina, Inc., San Diego, USA) platform to generate approximately 13 million reads per sample.