The callus was isolated from each mouse and dissociated into a single-cell suspension by enzyme digestion, single cells were suspended in calcium- and magnesium -free PBS containing 0.04% weight/volume BSA Single-cell suspensions were loaded to 10x Chromium to capture 5000 single cell according to the manufacturer's instructions of 10X Genomics Chromium Single-Cell 3' kit (V3) .The following cDNA amplification and library construction steps were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run,150bp)