ChIP-Seq for human TSCs was performed following our previous study (Sun et al. 2021, , PMID: 34320657). Chromatin fragmentation was performed using Diagenode Bioruptor Plus Sonicator. The antibody for H3K27ac (Abcam, ab4729) is used. The amount of chromatin is 20 μg per reaction. ChIP-Seq libraries were constructed using Takara SMARTer ThruPLEX DNA-Seq Kit (Takara, R400674), and sequenced as 50 bp paired-end reads with HiSeq2500 (Illumina) platform.