Cells were then lysed in TRIzol reagent (Invitrogen Cat# 15596026), RNA was isolated using Direct-zol RNA MiniPrep Kit (Zymo Research Cat# R2052) RNA-Sequencing libraries were prepared  using KAPA mRNA HyperPrep Kit (Roche Cat# KK8440), after quantification by Qubit (Invitrogen), barcoded samples were mixed and sequenced on an Illumina NovaSeq system.