The root tips (0.5 cm) of the seedlings were harvested for scRNA-seq experiment. The root tips were harvested and digested for 2 h at room temperature in digestion buffer (1.5% cellulase R10, 1.5% macerozyme R10, 0.3 M mannitol, 3 mM β-mercaptoethanol, 10 mM CaCl2, and 0.3% BSA). Single-cell suspensions were loaded onto 10× GENOMICS Chromium to capture approximately 6000 single cells following the manufacturer's instructions for the 10× Genomics Chromium Single-Cell 3′ kit (V3). cDNA amplification, library construction, and sequencing on the Illumina NovaSeq 6000 platform (paired-end multiplexing run, 150 bp) were performed by LC-Bio Technology (HangZhou, China)