RNA was extracted from thawed Tempus tubes using PerfectPure™ Blood RNA Kit (5 PRIME) or Norgen Preserved Blood RNA kits (for Tempus Tubes), including DNAse treatment. 1000 ng of the isolated total RNA was globin reduced, where indicated, using the Human Globinclear Kit and 200 ng of RNA was used to make cDNA libraries for sequencing using the MGIeasy RNA Library Prep with PE100 chemistry.