8ug/mL blasticidin and 4ug/mL puromycin, started 4 days after transduction Libraries for RNA-Seq were prepared with Nugen Universal plus mRNA-Seq Kit to generate strand-specific RNA-seq libraries.  The workflow consists of poly(A) RNA selection, RNA fragmentation and double-stranded cDNA generation using a mixture of random and oligo(dT) priming, followed by end repair to generate blunt ends, adaptor ligation, strand selection, and PCR amplification to produce the final library.  Different index adaptors were used for multiplexing samples in one sequencing lane. Sequencing was performed on NovaSeq 6000 for PE 2x150 bp run. Data quality check was done on Illumina SAV. Demultiplexing was performed with Illumina Bcl2fastq2 v 2.19.1.403 program.