The obtained samples are quickly placed into EP tube with PBSbuffer for flushing the blood. Then, the samples were cleanedin cell preservation solution. Finally, the cleaned samples wereplaced into a sterile EP tube with 2 mL cell preservationsolution before being stored in a refrigerator at 4 ° C. The cells were sorted as single-cells using the Chromium 10X Genomics platform. Approximately 7,000 cells were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 10x Single Cell 3' v3. Sequencing libraries were prepared using Single Cell 3' Reagent Kits v3 (10x Genomics)