Total RNA was extracted using Direct-zol RNA Miniprep Plus Kit from Zymo Research according to the manufacturer's instruction. 1ug of total RNA was used for the construction of sequencing libraries. Libraries were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module, Ultra II RNA Library Prep Kit, and Multiplex Oligos (NEB, Cat #E7490, E7775, E6609) according to the manufacturer's instruction.