RNA was extracted using TRIzol (Invitrogen, Carlsbad, CA) followed by QIAGEN RNeasy Midi Kit. RNA integrity and quality was evaluated using RNA QubitTM assay. Sequencing was performed using 150bp paired end sequencing strategy using Illumina sequencing platform and PE150 Technology The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.