Each heart sample was pooled from six Wtap-CKO and seven Wtapflox/flox mice at 4 days old, respectively.Nuclei was extracted from heart samples. Briefly, nuclei was extracted from samples, and the nuclei pellet was resuspended in the Tn5 transposase reaction mix.  The transposition reaction was incubated at 37℃ for 30 min. Equimolar Adapter1 and Adatper 2 were added after  transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the  AMPure beads and library quality was assessed with Qubit. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE  Cluster Kit v3-cBot-HS (Illumina) according to the manufactuer's instructions. After cluster generation, the library  preparations were sequenced on an Illumina platform and 150 bp paired-end reads were generated.