RNA was collected from cells lysed in TRIzol Reagent (Fisher Scientific #15-596-018) and isolated using the Direct-zol RNA Extraction Kit (Zymo Research #R2052). RNA libraries were generated using the KAPA Stranded mRNA-Sequencing Kit with KAPA mRNA Capture Beads (Roche #07962207001). Samples were enriched for poly(A)+ mRNA strands using mRNA Capture Beads. The captured mRNA was synthesized into complementary DNA (cDNA). cDNA libraries were ligated using Illumina TruSeq single index adapters (Roche #KK8700), amplified, and quantified using a Qubit dsDNA Broad Range Assay Kit (Life Technologies #Q32850) and a Qubit 2.0 fluorometer.