Orthotopic left lung transplantation was performed from a B6 (CD45.2) donor into a B6 (CD45.1) recipient. Grafts were stored for 60 minutes at 4 ˚C with an additional 45 minutes at 28˚C prior to transplantation. The left lung was allowed to reperfuse for either 2 hours or 3 days prior to sacrifice and lung harvest. For the naïve sample, the left lung was harvested from a naïve B6 mouse. Single cell suspension was prepared as described above. For each sample, cells from two mice were pooled and were stained with CD45.2 (clone 104; Biolegend), CD45.1 (clone A20; Biolegend) and DAPI (BD Biosciences, 564907). Flow cytometric analysis and sorting were performed on a BD FACS Melody cell sorter. Chromium Single Cell 5ʹ Reagent v1.1 kit from 10X Genomics and Chromium Single Cell 3ʹ Reagent v1.1 kit from 10X Genomics