1. Thaw the samples from -80°C freezer on ice, homogenize them by gentle pipetting and transfer them to 2mL PhaseLock tubes.  2. Add half the volume of Chloroform: Isoamyl alcohol (24:1, v:v Honeywell, Cat. No. C2432-25ml) to each lysate and shake vigorously for 15 second  3. Incubate the samples for 5 min at room temperature.  4. Centrifuge at 12, 000g for 5 min at 4°C and transfer the upper aqueous phase to a pre-chilled 1.5 mL microtubes.  5. Add 1.5 volumes of Isopropanol to each tube and mix well by pipetting.  6. Transfer the lysates to RNeasy MinElute® spin columns and extract total RNA using miRNeasy Micro Kit (Qiagen, Cat. No. 217084), with on-column DNAse treatment (Qiagen, Cat. No. 79254)  7. For the final elution step, use 14ul and 30ul of nuclease-free water to elute RNA from leukocyte and liver tissue sample respectively. Total RNA for cDNA synthesis following SMART-seq® v4 Ultra Low Input RNA kit's  instruction (Takara Bio, #634891)with a maximum of 10 amplification cycles. The final sequencing libraries were created with the tagmentation-based DNA Prep Kit (#20018705) and the IDT® DNA/RNA UD Indexes Set A (#20026121), both Illumina®.