RNA from cultured endothelial cells was prepared by phase separation using a TRIzol-chloroform method. Libraries were constructed using Illumina's TruSeq stranded mRNA library kit with unique dual indexing protocol and amplified for sequencing by qPCR (Kapa Library Quantification Kit, Kapa Biosystems). Sequencing produced 150-bp paired end reads across up to two lanes. Reads from samples run in multiple lanes were merged prior to quality control and trimming.