Total RNA was extracted using Arcturus® PicoPure RNA Isolation Kit (Thermo Fisher); GFP+ (proliferating) and GFP+CV+ (cell cycle-inactive) cells were sorted directly into extraction buffer after isolation and dissociation of brain tissue. Full length cDNA libraries were generated according to the published Smart-seq2 (Picelli et al., (2014). Nature Protocols. 9: 171-181), and with the Nextera XT DNA Library Prep kit (Illumina).