RNA was extracted using the PureLink RNA Mini Kit (Thermo). RNA libraries for in vivo tumors were prepped using the NEBNext Ultra II Directional RNA Library Prep with rRNA Depletion Kit (mouse) with at least 10ng of total RNA per sample. RNA libraries for in vitro 3D organoid samples were prepped with the NEBNext Ultra II Directional RNA Library Prep with poly(A) mRNA Isolation with at least 100ng of total RNA per sample.