About 10000 cells from each condition were loaded on to separate inlets of a 10 × Genomics Chromium controller to create GEM emulsions. The targeted recovery was 6,000 cells per condition Emulsions were used to perform reverse transcription, cDNA amplification, and preparation of the RNA-sequencing library Libraries were sequenced on an Illumina HiSeq 4000 platform, using 75 bp paired end reads and loading one sample per sequencing lane.