Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA purity and quantification were assessed using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was evaluated with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Transcriptome libraries were constructed using the VAHTS Universal V5 RNA-seq Library Prep Kit according to the manufacturer's protocol. Transcriptome sequencing and analysis were performed by OE Biotech Co., Ltd. (Shanghai, China).