A total of 50000 GFP positive cells were sorted, and RNA was extracted using the RNeasy Plus Micro Kit (cat. no. 74034) by Qiagen. RNA quality was assessed by Bioanalyzer and samples with a RIN value>8 were processed for library preparation. A total of 10ng of RNA was used to prepare libraries using the NEBNext® Low Input RNA Library Prep Kit (E6420L) following the manufacturer's instructions.