For WES, macro-dissected HCC were weighted and equal amounts (~1mg each) from end-stage (n=3 each genotype) HCC-bearing mice and control liver (n=3). Following DNA extraction and fragmentation (200-300 bp), a maximum of 1 μg of sheared DNA was used for library preparation using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234) following manufacturer's instructions. With an input of maximum 1 μg sheared DNA, library preparation for Illumina sequencing was performed using the KAPA HTP Prep Kit (KAPA Biosystems, KK8234). During library amplification 4 PCR cycles were used to obtain enough yield for the exome capture. After library preparation, the libraries were cleaned up using 1X AMPure XP beads. All DNA libraries are analyzed on a BioAnalyzer system using the DNA7500 chips for determining the concentration. A single pool of six samples were created using 142ng of each indexed sample. SeqCap ES Share Developer Mouse probe (Roche, ref 08333025001), following the Hypercap Target Enrichment (Roche, ref 08286370001) with the probe incubation for 20 hours. After capture the pool was amplified using 10 PCR cycles and purified with Ampure XP beads. The pool was diluted to a final concentration of 10nM and subjected to paired-end 100bp sequencing on an Illumina Illumina Novaseq 6000, according to manufacturer's instructions.