Using archived 4µm FFPE tissue sections from paired biopsies of non-responder and responder vitiligo lesions, we applied a novel method, TempO-Seq (Templated Oligo Assay with Sequencing Readout) (BioSpyder Technologies, Inc). We used transverse sections of FFPE slides from paired responding and non-responding lesions from 4 vitiligo patients (8 paired samples total). 1mm^2 epidermal scrapes were homogenized and lysed after dissolving paraffin. Most of the epidermis was collected, and there could be some minimum contamination with dermal tissue. The immense majority of cells collected were epidermal KCs, MCs, immune cells and Merkel cells present in epidermis. The resulting lysates were added to the TempO-Seq assay, where DOs were annealed, unbound DOs were enzymatically removed, and bound probes were ligated and amplified. The amplified assay products were pooled and purified, then directly sequenced using the Illumina NextSeq 550 platform. The whole transcriptome DO pools consisted of 22,537 DO pairs targeting 19703 genes. The 8 epidermal scrape samples were sequenced to an average depth of 5M reads (equivalent to ~50M reads in RNA-seq). All 8 samples achieved >3M mapped reads; there were no samples that failed to produce any usable data.