Cells were washed with ice-cold D-PBS, and total RNA was harvested using Arcturus® PicoPure® RNA Isolation Kit (Applied Biosystems). PolyA-enriched libraries were prepared for RNAseq and sequenced on an Illumina NovaSeq 6000 platform by NovogeneAIT to obtain 20M 150bp paired-end reads per sample. All samples had RNA integrity number (RIN) > 9.