Nitrogen liquid grinding followed by NEB Monarch Genomic DNA Purification Kit was used to extract the DNA of all samples. RNA was extracted using the Monarch Total RNA Miniprep Kit. We used 200 ng of RNA from the samples to build mRNA-seq libraries, first enriching for poly-A transcripts with the NEB Magnetic mRNA Isolation Kit S1550S, and then building the libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L) according to manufacturer's instructions.