The mycelia were collected, frozen in liquid nitrogen and  lyophilized. Total RNA was extracted  using the RNeasy midi kit (Qiagen). RNA was then treated  by DNase (Ambion) and RNA quality was verified using the Bioanalyzer RNA 6000 Nano kit (Agilent). 1.5µg was sent to Fasteris SA (Switzerland) for transcriptome sequencing. Libraries for RNA-seq were constructed using the Illumina TruSeq Stranded mRNA library kit .