RNA was extracted using RNeasy columns following the kit protocol (QIAGEN). Poly-A RNAs were enriched from 1ug total RNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and libraries were prepared using the KAPA Stranded mRNA-Seq Kit. Libraries were sequenced on a HiSeq4000 in Paired-End 50bp fragments with an average of 52M fragments per library.