RNA from cell lysates will be isolated using Tri-Reagent and RNA will be extracted and purified using DirectZol™ RNA MiniPrep kit (Zymo Research, Irvine, CA, USA), according to the manufacturer's instructons. DNase treatment was used to reduce the amount of genomic DNA. Ribosomal RNA (rRNA) was depleted from the samples using Ribosomal RNA depletion pools (riboPOOLs) developed by siTOOLs Biotech (Planegg, Germany), to enable sensitive detection of mRNA and non-coding RNA. RNA was reverse transcribed to cDNA fragmentation and size selection was performed to purify sequences that were the appropriate length for the sequencing machine. Once the RNA libraries were prepared, they were sequenced using a NextSeq 2000 instrument obtaining 30-40 million reads per sample.