RNA was extracted was carried out using a modified CTAB method (Pushkova et al., 2019, Barbier et al., 2019). 0.5ml of CTAB buffer (100mM Tris-HCL, pH9.5, 2% CTAB, 1.4M NaCl, 1% PEG 8000, 20mM EDTA, 2% PVP-40, 40mM DTT) and 2µl proteinase K was added to 50mg of ground tissue. This was incubated for 5 minutes at 65°C. 60µl of 10% SDS was then added, the tube inverted, and 1 volume of chloroform added. The samples were vortexed for 10 seconds, centrifuged (4°C, 5500xg,10 minutes). The aqueous phase was taken and mixed with an equal volume of chloroform. After centrifugation (room temperature, 14,000xg, 5 minutes), the aqueous phase was taken and precipitated overnight with 0.5 volumes of 7.5M Ammonium acetate and 2.5 volumes of ethanol. After extraction the RNA was treated with turbo DNAse (Invitrogen, AM2238) according to the manufacturer instructions. mRNA library preparation (poly A enrichment)