title: GSM8096431: 3B_R_2_rep2; Homo sapiens; RNA-Seq
library_name: GSM8096431
library_construction_protocol: mRNA high throughput sequencing was performed by Biotree (Shanghai, China). Briefly, total RNA was extracted using Trizol Reagent (ThermoFisher, 15596026). The mRNAs were purified using a NEBNext Poly(A) mRNA Magnetic Isolation Module Kit (NEB, E7490) according to the user manual. mRNA libraries were constructed by using Illumina TrueSeq mRNA sample prepa_x0002_ration kit (Cat. No. RS-122-2101) according to the manufacturer's instructions. Library sequencing was performed on an Illumina NovaSeq 6000 instrument.