title: GSM6456264: 66_007_negCD45; Mus musculus; ssRNA-seq
library_name: GSM6456264
library_construction_protocol: Total RNA of sorted cells was extracted using RNA MicroPrep Kit (Quick-RNA™ MicroPrep Kit  w/Zymo-Spin™ IC Columns, Nordic Biosite, Cat #43844) according to manufacturer's instructions. RNA quantity, quality/integrity and purity were evaluated using Qubit (Thermo Fisher Scientific, MA, USA), Bioanalyzer (Agilent, CA, USA) and NanoDrop (Thermo Fisher Scientific, MA, USA).  RNA sequencing was performed on RNA extracted from the sorted populations: 67NR CD45- (n=5), 67NR CD45+ (n=5), 66cl4 CD45- (n=5), 66cl4 CD45+ (n=5). Sequencing libraries were generated using SENSE mRNA-Seq library prep kit V2, according to the manufacturer's instructions (Lexogen GmbH, Vienna, Austria).