title: ="16S V4 Amplicon Sequencing of "&B486
design: Amplicon was obtained via PCR using the universal 16S V4 primer pair containing partial Illumina Nextera Adapter. The PCR product was purified and used as the template for 2nd PCR to incorporate dual-Index barcode. Sequencing was performed on an iSEQ100 ( 1 x 300 bp)
library_name: SK044_T02