title: GSM6940124: sc9, time-course analysis of senescence, bio rep2; Homo sapiens; RNA-Seq
library_name: GSM6940124
library_construction_protocol: RNA was extracted following SPLIT-seq protocol: after 3 rounds of barcoding, cells were incubated at 55C in buffer containing SDS and proteinase K for 2 hours. Liberated cDNA/RNA hybrids were then further processed through SPLIT-seq protocol. The libraries were prepared via SPLIT-seq method. Briefly the cells were barcoded through 3 rounds of combinatorial barcoding. Cells were then lyzed, the library was prepared via series of amplification, fragmentation, size selection, end repair and ligation and pcr-based indexing SPLIT-seq scRNAseq