title: DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - III
design: DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - III
library_name: nmtDbp2_2_818_t0_p
library_construction_protocol: Cells were grown in EMMG, then shifted to YES for 5h or 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before harvesting by centrifugation. Total RNA was extracted with the hot phenol method.  Libraries were constructed using the TruSeq protocol (Illumina) after ribodepletion with the Ribo-Zero Magnetic Kit for yeast (Epicentre).