title: RNA-seq of Arabidopsis thaliana root and shoot in response to elevated atmospheric CO2
design: RNA-seq of Arabidopsis thaliana root and shoot in response to elevated atmospheric CO2
library_name: Sample 7_p
library_construction_protocol: Samples were collected and directly frozen in liquid nitrogen Plants were grown in hydroponics under short-days conditions. Total RNA were isolated using DirectZol RNA kit (ZymoResearch) After the QC procedures, mRNA is enriched using oligo(dT) beads. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.