title: ITS2 rRNA sequencing P. damicornis fraction 25% 5
design: DNA was extracted using the AllPerp Kit. Symbiodiniaceae communities were characterized by sequencing of amplicons for the ITS2 gene. Fragments of approximately 270 base pairs (bp) were amplified by PCR using the primer pair SYM_VAR_5.8S2/SYM_VAR_REV (Hume et al., 2018 - doi:10.7717/peerj.4816). PCR products were sequenced using Illumina MiSeq technology (2 x 150 bp) at the Ramaciotti Centre for Genomics (UNSW, Sydney, Australia).
library_name: DF23-ITS_S100