title: GSM7668885: NCD_DW , replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_name: GSM7668885
library_construction_protocol: Freshly collected liver tissue from the NCD, HFrD mice were chapped in collagenase solution. After enzymatic digestion, the hepatic cells were homogenized using a GentleMACS Dissociator (Miltenyi Biotec). And then, the supernatants passed through a 70 µm nylon cell strainer (BD Falcon, Millville, NJ) for totally digestion. The cells centrifuged at 500rpm for 5 min at 4℃ and centrifuged once more with the supernatant to remove hepatocyte. After that, Red blood cells removed by RBC lysis procedure and hepatic mononuclear cells were isolated by percoll (Cytivia) gradient.