title: GSM7902704: U373 ADAR3 R-Mutant, IP, Rep 3; Homo sapiens; RIP-Seq
library_name: GSM7902704
library_construction_protocol: Prior to cell lysis and sonciation, cells were subjected to UV crosslining at 150 mj/cm2 using a Spectrolinker (Spectronic Corp) following which they were subjected to RNA-Immunoprecipitation. For all RIP-Seq libraries, RNA was first subjected to ribosomal RNA depletion using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) followed by library preparation using the KAPA Strand-Specific RNA Library Kit. For input samples, 1ug of total RNA was used as starting material. For each IP sample, total immunoprecipitated RNA was used. Libraries were amplified for 15 cycles using a thermocycler.