title: GSM8238056: unmod_T, first mixing experiment; Homo sapiens; Mus musculus; RNA-Seq
library_name: GSM8238056
library_construction_protocol: Cells were dissociated using Trypsin-EDTA, filtered through a cell strainer and resuspended in BD Rhapsody Sample Buffer Libraries were generated on a BD Rhapsody Express machine. Libraries for unmodified samples (unmod) were generated following manufacturers recommendations for the WTA assay. The protocol for v1 BD Rhapsody was used (i.e. a single RPE reaction) and applied to enhanced beads. The unmod_roi condition was generated following the RoCK and ROI protocol (without addition of the N1 primers). Briefly, the protocol differs from the standard WTA protocol due to the following points: 1) addtion of N1 primer to the RPE PCR (for unmod_n1 condition); 2) separate indexing reaction for the samples in which the N1 primer is added; 3) custom sequencing primer