title: GSM8416035: PccT3; Pectobacterium carotovorum subsp. carotovorum; RNA-Seq
library_name: GSM8416035
library_construction_protocol: For sequencing library preparation, 4 ml of bacterial cultures were harvested by centrifugation at 5000 × g for 10 minutes and then stabilized using the RNAprotect Bacteria Reagent (Qiagen, CA, USA). Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol. RNA quality and quantity were measured with an Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit (New England Biolabs, MA, USA). The enriched RNA was then used to construct cDNA libraries with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, CA, USA) according to the manufacturer's protocol. Paired-end NGS sequencing was performed on the Illumina NovaSeq 6000 platform (Macrogen, Seoul, Korea).