title: GSM6733513: Cortex_het4_bsseq; Mus musculus; OTHER
library_name: GSM6733513
library_construction_protocol: In vitro transcribed Luciferase Control L4561 RNA was used as negative control in the amplicon-bsRNA-seq experiments. RNAs were transcribed using the MEGAscript® T7 Kit (Life Technologies) according to the manufacturer's protocol. Total mRNA of mouse cortex was extracted by the Ribo-Zero rRNA depletion kit. Then 1 μg of the mRNA combined with 1 ng Control L4561 RNA  were used for bisulfite treatment by EZ Methylation Kit (Zymo) following the protocols provided by the manufacturer. After bisulfite conversion, the purified RNA was used for library construction by NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB).