title: GSM6390469: glh-2 degron rep2; Caenorhabditis elegans; ncRNA-Seq
library_name: GSM6390469
library_construction_protocol: Total RNAs were Trizol and isopropanol precipitation, small RNAs were enriched using mirVana miRNA isolation kit. Purified small-RNAs were pre-treated with PIR-1 and ligated with 3' and 5' linkers. cDNA were generated by reverse transcribed and further amplified by PCR. A size selection procedure on native PAGE were carried out.