title: GSM8005623: PBMC, SA1; Homo sapiens; RNA-Seq
library_name: GSM8005623
library_construction_protocol: Total RNA was extracted from the PBMC samples using the Total RNA Extraction Reagent (Vazyme biotech co., Ltd, R401), according to the manufacturer's instructions. The integrity of the extracted RNA was assessed through agarose gel electrophoresis, while the quantity and quality were evaluated using the NanoDrop ND-1000. For library construction, 1 μg of the total RNA was utilized. To remove rRNA, the RiboZero Magnetic Gold Kit (Epicentre, an Illumina Company) was employed. Subsequently, the KAPA Stranded RNA-Seq Library Prep Kit (Illumina) was used to construct the RNA-sequencing library. All procedures were conducted following the manufacturer's instructions