title: Study on the mechanism of enhanced anaerobic ammonia oxidation performance by extracellular electron acceptor biochar
design: Anaerobic ammonia oxidation process has the advantages of energy and cost reduction, therefore, it has been considered as one of the main alternatives to conventional biological denitrification process in recent years. Biochar has been applied in the anammox process for nitrogen removal effificacy. But, due to its extracellular electron transfer capacity and abundance of redox-specific functional groups, which served as extracellular electron acceptor to anaerobically oxidize NH4+ is still controversy. In this study, the anaerobic ammonia oxidation was investigated when biochar was used as an electron acceptor in the wastewater. According to the optimal process variables determined in the batch tests, when the influent NH4+-N concentration in the anaerobic ammonia oxidation reaction was 30-50 mg/L and the biochar dosing was at 10g/L, it showed some promotion in the long-term experiments. The anaerobic ammonia oxidation process with biochar as the electron acceptor reached more than 60% NH4+-N removal efficiency in the system, and the NO3--N/NH4+-N ratio reached 0.19 which tended to the theoretical value. After 20 days, the voltage in the system keeps fluctuating about 4mV, indicated that the functional bacteria using biochar as the electron acceptor gradually dominated the system. In addition, the abundance of norank_f__norank_o__SBR1031 in the Chloroflexi phylum has increased significantly at 29.92%, while the abundance of the major genus Candidatus_Kuenenia in AnAOB has decreased slightly at 11.47%.
library_name: A2(anaerobic digester metagenome)