title: GSM7413256: SRSF2_KO; Mus musculus; RNA-Seq
library_name: GSM7413256
library_construction_protocol: The diaphragms were isolated from one day old WT, SRSF1 KO and SRSF2 KO mice immediately following euthanasia. To obtain single cells, the samples were minced and placed in enzymatic buffer containing dispase II and collagenase XI at 37 ℃ for 30 min. After termination of digestion, the samples were filtered through 40um cell strainers, centrifuged and resuspendend in 20% FBS/F-10 solution. Then the single cells were used for single-cell sequencing. RNA libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.