title: GSM8232583: BrdU-ip-seq from RPE1 cells grown in 0 µM BrdU [IP]; Homo sapiens; OTHER
library_name: GSM8232583
library_construction_protocol: DNA was extracted using classical phenol:chloroform and precipitation techniques. Immunoprecipitated DNA was purified using AMPure XP beads at 0.9x. IP and input DNA were amplified separately using Illumina compatible indexes and NEBNext Ultra II Q5 Master Mix (M0544) for an equal number of cycles, typically 15-17, depending on recovery, and purified using AMPure XP beads at 0.9x.