title: 16S rRNA gene amplicon of bovine: rumen liquid
design: Rumen samples were collected by using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by using Qiagen Dneasy Power Soil Pro kit. For microbial amplicon sequencing universal primers targeting 16S rRNA gene V4 region were used (Caporaso et al. 2011).
library_name: 402RB