title: GSM6734029: Sample_VEH2-75_IGO_12061_178; Mus musculus; RNA-Seq
library_name: GSM6734029
library_construction_protocol: Cells were sorted into Buffer RLT (Qiagen catalog # 79216) and RNA was purified using the Agencourt RNAClean XP beads (Beckman Coulter catalog # A63987) at a 2.2X ratio. First-strand cDNA synthesis was achieved using Maxima H Minus Reverse Transcriptase (ThermoFisher catalog # EP0751) according to the manufacturer's protocol using oligo dT primers, plus the addition of a custom template-switch oligo in a 1mM final concentration. cDNA was amplified for 24 cycles using KAPA HiFi HotStart ReadyMix (Kapa Biosystems KK2601). After PicoGreen quantification, 0.1-20 ng of cDNA was used to prepare libraries with the Nextera XT DNA Library Preparation Kit (Illumina catalog # FC-131-1024) in a total volume of 6.25 µL with 12 cycles of PCR. Indexed libraries were pooled by volume and cleaned by aMPure XP beads (Beckman Coulter catalog # A63882) at a 2X ratio.