title: GSM6696828: RM-1wk replicate 1; Homo sapiens; ChIP-Seq
library_name: GSM6696828
library_construction_protocol: Chromatin immunoprecipitation (ChIP) was performed as previously described (Sahu et al. EMBO J 2011). Cells were ﬁxed in 1% formaldehyde for 10 min at RT. Glycine was added to a final concentration of 0.125 M and incubated for 5 min to quench the reaction. Cells were then washed twice with ice-cold PBS and collected for lysis in RIPA buffer with protease inhibitors. Cross-linked chromatin was sonicated to an average fragment size of 200-500 bp, then it was immunoprecipitated with either H3K27ac antibody (Diagenode, #C15410196) or normal rabbit IgG (Santa Cruz, #sc-2027), 2μg of antibody per reaction. Libraries were prepared as previously described (Sahu et al. EMBO J 2011) according to Illumina's instructions.