title: GSM8282465: Colon cancer EVs, 40T, sEVs; Homo sapiens; RNA-Seq
library_name: GSM8282465
library_construction_protocol: RNA contained in EVs was isolated using the miRNeasy mini kit (QIAGEN, Hilden, Germany), following the manufacturer's protocol. Small EVs (5 × 10^8 particles), large EVs (5 × 10^7 particles) were used to extract RNA. RNA libraries were constructed using the NEBNext Small RNA Library Prep Set for Illumina (NEB) in accordance with the manufacturer's instructions, and libraries were sequenced on the HiSeq 2500 platform (Illumina) in 75-base pair single-end reads.