title: GSM6940969: CLIP_Input; Homo sapiens; OTHER
library_name: GSM6940969
library_construction_protocol: FLAG/Spy-tagged PQBP1 overexpression HEY cells were irradiated at 400 mJ/cm2 using UV Crosslinker (UVP, CL-1000). Nucleoprotein Extraction Kit (Sangon) was employed to extract nuclei before lysis. DNA was removed by Turbo DNase (2 U/µl, Invitrogen, AM2238) and RNA was fragmented with 1:200 diluted RNase I (100 U/µl, Invitrogen, AM2295). Anti-FLAG magnetic beads (MBL, M185-11) were added to the mixed lysate for 1 h at 25 °C. The beads were washed stringently following the protocols and digested with proteinase K (Roche, 3115828001). RNA was purified using the Spin Column RNA Cleanup & Concentration Kit (Sangon), and the library was constructed and sequenced by the Ribobio Biotechnology Company with the NEBNext Ultra RNA Library Prep Kit for Illumina. CLIP-Seq