title: GSM6733366: A1; Homo sapiens; RNA-Seq
library_name: GSM6733366
library_construction_protocol: Uterine muscle layer tissue was dissected into five segments and the single cell suspension was obtained by enzymatic digestion. The cell pellet was resuspended in 50 μl of 1× PBS (0.04% BSA). The overall cell viability was confirmed by trypan blue exclusion , which needed to be above 85%, single cell suspensions were counted using a haemocytometer/ Countess II Automated Cell Counter and concentration adjusted to 700-1200 cells/μl. Single-cell suspensions were loaded to 10x Chromium to capture 8000 single cell according to the manufacturer's instructions of 10X Genomics Chromium Single Cell 3'  Reagent Kits（v3）.The following cDNA amplification and library construction steps were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system