title: GSM6940500: HPMCs from patient 2823 treated with ascites sample1; Homo sapiens; RNA-Seq
library_name: GSM6940500
library_construction_protocol: Total RNA was isolated from primary human mesothelial cells 36 hours after treatment with Tyk-nu spheroid conditioned media, ascites, or control media using RNeasy Mini kit (Qiagen) following manufacturer's instructions. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN). RNA integrity and quantitation were assessed using the RNA Nano 6000 assay kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Library preparation and next generation RNA sequencing was carried out by Novogene Sequencing libraries were generated using NEBNext® Ultra™ RNA library Prep kit for Illumina® (NEB, USA) and index codes were added to attribute sequences to each sample. The library quality was assessed on the Agilent Bioanalyzer 2100 system. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina). After cluster generation, the library preparations were sequenced on an Illumina platform and paired- end reads were generated