title: GSM8393929: SpRY-ABE-HTT-3_OT2_biorep_3; Homo sapiens; OTHER
library_name: GSM8393929
library_construction_protocol: DNeasy DNA extraction; amplicon sequencing with Nextera XT library consruction; MiSeq; CRISPResso2 alignment and quantification of base editing and nucleotide frequency Samples for DNA editing were extracted using Qiagen's DNeasy DNA extraction kit Samples for total RNA editing were extracted using Qiagen's RNeasy plus kit For Illumina Miseq experiments, libraries were constructed with the nextera XT indexing primer kits as per manufacturer's instructions Targeted genomic and exon high throughput sequencing with nextera indexing primers