title: GSM7413027: P2, VisiumFFPE; Homo sapiens; RNA-Seq
library_name: GSM7413027
library_construction_protocol: Paraffin-embedded tissue sections from patients with giant cell arteritis and granulomatous with polyangiitis were used. The samples were mounted onto Visium Spatial Gene Expression Slides (10x Genomics), fixed in ice-cold methanol, and stained. Tissue permealization was then performed to extract mRNA according to the Visium Spatial Gene Expression user guide The Visium Spatial Gene Expression Reagents Kit (10x Genetics) was used to generate spatially barcorded cDNA libraries following the manufacturer's instructions. Optimal experiment conditions were investigated using the Visium Spatial Tissue Optimization Kit (10x Genomics).