title: GSM7875135: QZH2; Mus musculus; RNA-Seq
library_name: GSM7875135
library_construction_protocol: Subcutaneous adipose tissues were dissected, minced, and digested with 1X Hank's balanced slated solution contained 1mg/ml Collagenase D (Roche, cat# 11088882001) and 1.5% BSA at 37°C for 45 min within a shaking water bath. Subsequently, 10mM EDTA was added and incubated for another 5 min. The digested mixture was then filtered through 100 μm cell strainer and then 40 μm cell strainer. Red blood cells were lysed with 1X RBC lysis buffer. Cells were resuspended with FACS buffer (2% FBS/PBS) and incubated with anti-mouse CD16/CD32 Fc Block (1:200) for 15 min on ice, and then stained with antibodies for 30 min on ice. Cells were washed twice and sorting was conducted by using a BD Biosciences FACSAria cytometer. Library prepared followed by 10X Genomics standard protocol.