title: Manihot esculenta RNAseq
design: RNA extraction was performed using 200-400 mg of petioles and CTAB (N-Cetyl-N,N,N-Trimethyl-Ammonium Bromide. Merck) as previously described (Jimnez et al., 2021) and quantified using Nanodrop 2000c spectrophotometer (ThermoFisher, USA). Quality was assessed with RNA OD260/280 ratio OD230/280 and visualized using 1% agarose gel electrophoresis.  Total RNA was sequenced with Illumina NovaSeq 6000 platform (Novogene, USA) using a 150 bp paired-end whole transcriptome sequencing protocol.
library_name: D2_1