title: Genome-sequence of USA 300 SAE (South American Epidemic Clone)
design: Strains were grown overnight in tryptic soy broth at 37 C. Genomic DNA was extracted using the DNeasy tissue kit (Qiagen, Hilden, Germany), and lysostaphin (100 mg/L; Sigma, Taufkirchen, Germany) for bacterial lysis. DNA quantification was carried out using the Qubit dsDNA HS Assay Kit (Invitrogen/Thermo Fisher Scientific, Karlsruhe, Germany). A total of 1 ng of extracted DNA was employed for library preparation using the Nextera XT DNA Library Prep Kit according to the manufacturers instructions (Illumina, San Diego, CA, USA).
library_name: S65