title: GSM7902855: HK1-WT_Control; Homo sapiens; ATAC-seq
library_name: GSM7902855
library_construction_protocol: Cell lysis occurred using a cold lysis buffer comprising 10mM Tris-HCl (pH 7.4), 10mM NaCl, 3mM MgCl2, and 0.1% IGEPAL CA-630. Nuclei were promptly spun at 500 × g for 10 minutes at 4℃, with subsequent discarding of the supernatant. After purification, library fragments were amplified using 1x NEBnext PCR master mix and 1.25 μM custom Nextera PCR primers. The PCR conditions involved an initial step at 72°C for 5 minutes, followed by denaturation at 98°C for 30 seconds, and subsequent thermocycling at 98°C for 10 seconds, 63°C for 30 seconds, and 72°C for 1 minute. A final extension step was performed at 72℃ for 5 minutes. The libraries were then purified using a Qiagen PCR cleanup kit, resulting in the final library.