title: GSM7903233: Mtsoc1a single mutant leaf bio rep 1; Medicago truncatula; RNA-Seq
library_name: GSM7903233
library_construction_protocol: Fully expanded trifoliate leaves (leaf) or shoot apices (apex) (15-100 mg), were harvested from plants grown under VLD conditions at Zeitgeber time 4 (4 h after dawn). Tissues were snap frozen with metal beads and homogenised using a Geno/Grinder (New Jersey, USA) into powder in liquid nitrogen. Total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen, Germany) following the manufacturer's protocol. The quantity and quality of RNA were checked by Bioanalyzer 2100 (Agilent Technologies, USA). The RNA of Mtsoc1a mutant and wild type R108 were constructed into 12 strand-specific mRNA libraries with polyA enrichment and were subjected to Illuminia NovaSeq 6000 paired-end 150 bp sequencing by Novogene. The RNA of triple Mtsoc1-4 mutant were constructed into three strand-specific mRNA libraries with polyA enrichment and sequenced on Illumina platform NovaSeq 6000, paired-end 150 bp by Novogene (Hong Kong).