title: GSM7670495: UY031, soil, biol rep 2; Ralstonia solanacearum; RNA-Seq
library_name: GSM7670495
library_construction_protocol: RNA PowerSoil® Total RNA Isolation Kit (MO BIO) was used supplemented with a Rigurous DNAse treatment using the TURBO DNA-free kit (Life Technologies, Ambion) Total RNA was extracted using the SV Total RNA Isolation System kit (Promega) for water samples and the RNA PowerSoil® Total RNA Isolation Kit (MO BIO) followed with a rigorous DNAse treatment with the TURBO DNA-free kit (Life Technologies) for soil samples. Bacterial rRNA was removed using NEBNext rRNA Depletion kit (Bacteria) (NEB). Libraries were independently prepared with 1ug of total RNA for each sample by Illumina TruSeq Stranded mRNA Sample Prep Kit