title: GSM8096343: Control_G7_Sol; Mus musculus; RNA-Seq
library_name: GSM8096343
library_construction_protocol: Muscle tissues were homogenized with Trizol reagent (15596018, Invitrogen) and RNA was isolated using MaXtract high density tubes (129056, Qiagen) following the manufacturer's protocol. Libraries were prepared using the INCPM-mRNA-seq protocol. Briefly, the polyA fraction (mRNA) was purified from 500 ng of total input RNA followed by fragmentation and the generation of double-stranded cDNA. After Agencourt Ampure XP beads cleanup (Beckman Coulter), end repair, A base addition, adapter ligation, and PCR amplification steps were performed.