title: Ectomycorrhizal fungal community responses to warming and rainfall reduction differ between pine host species but are similar across experimental cohorts
design: To identify the ectomycorrhizal fungi present on pinaceae host roots in the B4Warmed experiment, total genomic DNA was extracted using the chloroform extraction method detailed in Fernandez et al. (2017). The ITS2 rDNA subunit was PCR amplified using a barcoded fungal-specific ITS5.8SFUn-ITS4Fun primer set under cycling conditions detailed in Taylor et al. (2016) using 35 cycles. Amplified products were magnetically cleaned using the Charm Just-A-Plate kit (Charm, San Diego, CA, USA). Each of the root samples were pooled into a single library and sequenced at the University of Minnesota Genomics Center using the 2 x 300 bp paired-end MiSeq Illumina platform.
library_name: DN.B4W.37099.069