title: GSM7876007: Cortex4_FW; Mus musculus; RNA-Seq
library_name: GSM7876007
library_construction_protocol: Total RNA was extracted from Cortex of 12 mice using TriReagent (Sigma) and the ISOLATE II RNA Mini Kit (BIOLINE). (sixTbl1xr1Y446C/Y446Cmice and six wild-type mice; three males and three females of each genotype). In order to create cDNA libraries, samples were prepared using the Kapa mRNA Hyperprep kit (Roche, Basel, Switserland). Concentration of the library samples was measured using the Quant-it DNA HS assay (Thermo Fisher Scientific, Waltham, MA, USA). Libraries were equimolar pooled and the pool was checked on Tapestation using DNA1000 ScreenTape (Agilent Technologies, Santa Clara, CA, USA). The libraries were sequenced on the NovaSeq6000 PE150 sequencer (Illumina, San Diego, CA, USA), producing at least 40M 150-bp paired-end reads per library.