title: GSM6634773: PD-1- T-ALL cells, Pten null, RNA-seq 3; Mus musculus; RNA-Seq
library_name: GSM6634773
library_construction_protocol: Fresh primary T-ALL cells were isolated by Ficoll gradient centrifugation to obtain mononuclear cells, and sorted by FAC sorter (InFlux Cell or FACSARIA III, BD Biosciences), BM cells were markered as SSCAlowCD45dimPD-1- or SSCAlowCD45dimPD-1+. Total mRNA(1 million cells ) was isolated using the RNeasy plus mini kit (74134, QIAGEN) and Performed transcriptome sequencing. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.