title: 16srRNA Metagenomics of Artmisia judica: Root
design: For 16S rRNA MiSeq sequencing, begin with DNA extraction using a kit like Qiagen DNeasy, followed by quantification. Amplify the 16S rRNA region (e.g., V3-V4) using specific primers and PCR. Clean up PCR products and proceed to library preparation using a kit like Illumina TruSeq, including end-repair, A-tailing, and adapter ligation. Add indexes for multiplexing and clean up the library. Quantify the library concentration using kits like Kapa. Dilute libraries for sequencing with the MiSeq Reagent Kit on the MiSeq instrument.
library_name: TCR