title: WGS for B16-OVA and 4T1 murine cancer cell lines
design: WGS for B16-OVA and 4T1 murine cancer cell lines
library_name: Sample 1_s
library_construction_protocol: After harvesting cells, we washed 3x with PBS (phosphate buffer saline, Gibco DPBS, no calcium, no magnesium, 14190-144, Fisher Scientific P/N 12559069), centrifuged at 2000 g, discarded supernatant and snap froze the cell pellets until further use. After thawing the cell pellets, aliquots of about 5 million cells were taken separately for DNA/RNA extraction and proteomics analysis. B16/OVA cells were grown (24-36h doubling period) in RPMI 1640 (cat. No. 42401 GIBCO) medium supplemented with 10% heat inactivated fetal bovine serum (cat. No. 10500 GIBCO), 1% Penicilin-Streptomycin (cat. No. 15140 GIBCO) and 1% glutamine (GlutaMAX cat. No. 35050 GIBCO). B16/OVA cells were kept in selection with 10% Geneticin Selective Antibiotic (G418 Sulfate) (cat. No. 10131 GIBCO) and medium was changed every two/three days.  Murine mammary carcinoma 4T1 cells were grown (12h doubling period) in RPMI 1640 high glucose (cat. No. A1049101 GIBCO) medium supplemented with 10% heat inactivated fetal bovine serum (cat. No. 10500 GIBCO) and 1% Penicilin-Streptomycin (cat. No. 15140 GIBCO).  All cell lines were cultured at 37°C in 5% CO2 in a humidified atmosphere. All cells were tested for Mycoplasma contamination using MycoAlert kit (Lonza, cat. LT07-118).  Qiagen all prep DNA/RNA extraction kit (Qiagen, cat. No. 80284) was used to extract DNA and RNA from the mouse cancer samples according to the manufacturer's instructions.  Input: DNA; Type: Illumina TruSeq PCR-free; Options: 350bp, -, 1291