title: GSM6697059: Patient34; Homo sapiens; RNA-Seq
library_name: GSM6697059
library_construction_protocol: RNA was then isolated from these tumor specimens. Total cellular RNA was extracted from primary tumor tissue using the mirVana (Thermo Fisher, Waltham, USA) RNA purification kit. The RNA yield and quality were assessed with Trinean Dropsense 16 spectrophotometer and Agilent Model 2100 bioanalyzer. RNA quality was determined to be adequate if the sample had an RNA integrity number (RIN) of 7.0 or greater. Samples that were of adequate quality were then sequenced. 500ng of RNA was quantified by Qubit measurement (Thermo Fisher). RNA was then converted to cDNA and ligated to sequencing adaptors with Illumina TriSeq stranded total RNA library preparation (Illumina, San Diego, CA, USA). cDNA samples were then sequenced with the Illumina HiSeq 4000 genome sequencing platform using 150 bp paired-end SBS chemistry. All sequencing was performed at the Genome Facility at the University of Iowa Institute of Human Genetics (IIHG).