title: GSM8185795: AEL76, scRNA; Drosophila melanogaster; RNA-Seq
library_name: GSM8185795
library_construction_protocol: Eye discs from Drosophila larvae were dissected in 1X PBS on ice and dissociated using collagenase and dispase for ~40min. The dissociated cells were washed once with 1X PBS with 0.05% BSA. The cell pellet was resuspended in 1xPBS+0.05% BSA and viability was asayed using Hoechst-PI Library was prepaed as per the manufacture's instructions. Single Cell 3' Reagent Kit v3.1 from 10x Genomics. Drosohila larval eye single cells were resuspended on master mix and GEMs were generated by loading the cells with partioning oil and gel beads with primers. The poly-A RNA was captured on the beads and reverse transcribed to generate cDNA which contains Illumina R1 primer sequence,10X barcode and a unique molecular identifier. The cDNA was purified and amplified by PCR. Illumina R2 primer sequence, paired-end constructs with P5 and P7 and sample index were added during library construction.