title: GSM6456459: INVEN_APHSpleen_Alpha5Pr_Capture_Sample 3; Mus musculus; OTHER
library_name: GSM6456459
library_construction_protocol: To produce CD71+ erythroid cells in vitro from differentiated mESCs (WT or mutant mouse ESC line): 24h prior to differentiation, mESCs were induced by passaging into IMDM base media, trypsinised and plated in differentiation media (IMDM suplemented serum and other reagents and growth factors) in either triple vent petri dishes (Thermo Fisher) or flat-bottom 96-well plates (Thermo Fisher) at 3x104 cells in 10 cm dishes or 250 cells per well of a 96-well plate for up to seven days without disruption. EBs were harvested and disaggregated in 0.25% trypsin for 3 minutes at 37°C. CD71-positive cells were isolated by magnetic column separation (LS Column, Miltenyi), according to the manufacturer'