title: GSM8239646: Skin, TRPV1 activation,  CD45+, Chrm RNA; Mus musculus; RNA-Seq
library_name: GSM8239646
library_construction_protocol: Back skin from TRPV1hM3Dq and control mice (three mice per group) 3.5 hours after a single CNO intradermal injection, were dissected, subcutis was removed mechanically and the dermal-epidermal fractions were digested. TotalSeq™ anti-mouse Hashtag antibodies were used to mark the different mice before pooling all control and TRPV1 activated samples together for staining and sorting. CD45+ were sorted. The dermal-epidermal and subcutis fractions from TRPV1 activated and control samples were pooled together and sorted using a BD FACSAriaII with a 100-μm nozzle into RPMI medium supplemented with 10% FBS. Single cells were quantified using a hemocytometer. Cell capture, cDNA library preparation, quality control, and sequencing was performed at UC Irvine Genomic High Throughput Facility. SingleCell 3'- 10x