title: Dactylorhiza majalis, leaf sample 3
design: The libraries were constructed using the TruSeq Stranded mRNA kit manufacturers protocol (Illumina, San Diego, CA, United States) according to the producer advices. They were then sequenced on a NovaSeq (Illumina, San Diego, CA, United States) platform in a paired-end mode with read length of 150 bases. After quality trimming with Trimmomatic (v0.36, parameters: PE and ILLULINACLIP:TruSeq3-PE.fa:2:30:10:2:true LEADING:21 TRAILING:21 MINLEN:30; Bolger et al., 2014), residual ribosomal RNAs were filtered out with SortMeRNA (version 2.1, against the databases silva-bac-16s-id90, silva-bac-23s-id98, silva-euk-18s-id95, and silva-euk-28s-id98 with the parameters  e 1e-07  paired_in).
library_name: DM01l_L2p