title: GSM7207915: RNAseq-T-E145-Col2a1GFP-GFPn-R1; Mus musculus; RNA-Seq
library_name: GSM7207915
library_construction_protocol: After sorting, cells were centrifuged for 5′ at 400 × g at 4 °C, supernatant was discarded, and cells frozen at −80 °C. Two biological replicates of 1.5 × 105 cells each were used to extract total RNA using RNeasy Micro Kit (Qiagen,169030531). SMART-Seq v4 kit (Clontech, 634893) was used for the reverse transcription and cDNA amplification according to the manufacturer's specifications, starting with 5 ng of total RNA as input. 200 pg of cDNA were used for library preparation using Nextera XT (Illumina, FC-131-1096). Libraries were sequenced on an Illumina HiSeq 4000 with single-end reads of 50 bases.