title: Lung Immune Cells in Wildtype and Cela1-/- Mice 21 Days After Tracheal Porcine Pancreatic Elastase
design: Lung Immune Cells in Wildtype and Cela1-/- Mice 21 Days After Tracheal Porcine Pancreatic Elastase
library_name: WTPPE_p
library_construction_protocol: mice were sacrificed by exsanguination, lungs collected and placed on ice, minced, digested in collagenase, elastase, dispase, DNAse, and Lich enzyme mix at 4oC, RBCs lysed, and passed through 30 micron filter, and single cells suspended and labeled with 10X Cellplex lipid-conjugated oligos. Dead cells were excluded using DAPI and flow cytometry with 50 micron nozzle which selected for smaller, immune cells. cDNA libraries were created from GEM using a 10X Chromium controller after loading 16,000 cells per well into 4 wells. cDNA libraries were created using  the v3.3 protocol, the Dual Index TT and NN kits, and 16 cycles. Feature barcode libraries were created using a 10X accessory kit