title: GSM6568113: Embryonic forelimb field, e1.5, rep 1; Gallus gallus; RNA-Seq
library_name: GSM6568113
library_construction_protocol: Forelimb field tissues were microdissected from the body, lateral to the somites. Library was performed according to the manufacter's instructions (single cell 3' V3.1, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode.