title: GSM6634674: MZ_290_WT; Mus musculus; RNA-Seq
library_name: GSM6634674
library_construction_protocol: Total RNA from TGCs was isolated using TRIzol (#15596026, Thermo Fisher Scientific). rRNA was depleted using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB # E6310L). cDNA synthesis was performed using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB # E7335S). Sequencing was performed using an Illumina NextSeq 500  with 75bp single read runs.