title: GSM6564293: HTPC2_noFCS; Homo sapiens; RNA-Seq
library_name: GSM6564293
library_construction_protocol: HTPCs were derived from small testicular samples of two donors, with obstructive azoospermia and normal spermatogenesis, and were isolated using tissue-explant culture. In a concentration of ~1,000 cells/µl, 10,000 cells were processed using the Single-Cell 3' Reagent Kits v2 from 10xGenomics according to the manufacturer´s instructions. This was followed by GEM generation and barcoding, post-GEM-RT cleanup, cDNA amplification, library construction and quality assessment with the Bioanalyzer (Agilent).