title: 18S rDNA based molecular profiling of Neopyropia yezoensis in China
design: The genomic DNAs of the samples were extracted by using the Super Plant Genomic DNA Kit (for polysaccharide- and polyphenol-rich plants) (DP360, Tiangen, Beijing, China). The concentration and purity of the DNAs were assessed by a nucleic acid protein detector and 1% agarose gel electrophoresis.The primer sequences for 18S rDNA: Forward: 5- CAGAGCGCTTTGAGATGATTC-3; Reverse: 5- CCCGACAAGAGGAGACAAACT-3. The 25 L reaction system was composed of 2 L DNA, 0.1 L each primer, 12.5 L 2Rapid Taq Master Mix (Sangon Biotech, China) and 9.5 L ddH2O. The PCR reactions were conducted on a gradient thermocycler (Bioer Technology, China) using the programme: initialization at 95 C for 3 min, followed by 35 cycles of denaturation at 94 C for 15 s, annealing at 53 C 15 s, and extension at 72 C for 30 s, then a final extension at 72 C for 5 min.
library_name: Neopyropia_18S rDNA