title: GSM7413413: Exp2_halfparS25_Rep2; Pseudomonas aeruginosa; RNA-Seq
library_name: GSM7413413
library_construction_protocol: Total RNA was isolated from three independent replicates of each strain. 2 ml of cultures which reached optical density 0.4-0.6 at 600 nm were mixed with 4 ml of RNAprotect Bacteria Reagent (Qiagen). RNA was isolated with an RNeasy mini-kit (Qiagen) according to the manufacturer's protocol. Total RNA was digested with DNase (TURBO DNA-free Kit, Ambion) to eliminate genomic DNA. rRNA was depleted using Ribo-Zero rRNA Removal Kit (Bacteria) (MRZMB126, Illumina) according to manufacturer instructions. Libraries were prepared according to instructions accompanying the NEBNext Ultra DirectionalRNA Library Prep Kit for Illumina (NEB, E7420). Libraries were sequenced using standard Illumina protocols.