title: GSM6567953: AP, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_name: GSM6567953
library_construction_protocol: Twenty C57/B6 male mice with apical periodontitis were used to acquire single-cell suspensions. Mandibles were dissected under a stereo microscope 19 (Leica). Specifically, soft tissues, molars, incisors and bone behind the condyle were removed. Then, mandibular alveolar bone tissue was cut into small pieces and digested with 3 mg/mL collagenase type I (Gibco) and 4 mg/mL dispase II (Sigma) for 60 min at 37°C as previously described (Cui et al., 2020; Yamaza et al., 2011). Red blood cells were lysed with 1 mL Red Cell Lysis Solution (Biosharp). Cells were centrifuged and resuspended in 1% BSA solution. The final concentration of cells was 1 x 10^6 cells per mL. Cellular suspensions were loaded into Chromium microfluidic chips and barcoded with a 10x Chromium Controller (10x Genomics). Cellular suspensions were loaded into Chromium microfluidic chips and barcoded with a 10x Chromium Controller (10x Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' v3 reagent kit (10x Genomics) and sequenced with the NovaSeq system (Illumina).