title: GSM7093228: R710000551 AATD CD45neg Rep1; Homo sapiens; RNA-Seq
library_name: GSM7093228
library_construction_protocol: Peripheral samples: 2cm deep from the pleura, airway or vessel visible structures >2mm removed. Proximal airway: 5 generations of airway from main stem bronchus.Cellular suspensions were loaded on a GemCode Single Cell Instrument (10x Genomics, Pleasanton, CA) to generate single cell GEMs. Approximately ~1200 to 2800 cells were loaded per channel. Two or one biological replicates were generated per sample. Single cell RNA-Seq libraries were prepared using GemCode Single Cell 3' Gel Bead and Library Kit (now sold as P/N 120230, 120231, 120232, 10x Genomics) following the protocols outlined in Zheng et. al. Sequencing libraries were loaded at 2.1pM on an Illumina Hiseq 2500 with 2 × 100 paired-end kits using the following read length: 98bp Read1, 14bp I7 Index, 8bp I5 Index and 5bp Read2. Libraries were prepared according to the manufacturer's protocol using Chromium Single Cell 3' v2 chemistry.