title: GSM6733556: CO8-DA-treated pea roots, 24 h 3; Pisum sativum; RNA-Seq
library_name: GSM6733556
library_construction_protocol: Extraction of RNA from the roots was done using the PuroZOL RNA isolation reagent (BioRad Laboratories, California, USA). DNA was eliminated using DNAse digestion (Thermo Fisher Scientific, USA). RNA quality and quantity were determined by Nanodrop (Implen GmbH, Munich, Germany). The RNA sequencing libraries were prepared following the guidelines for NEBNext mRNA magnetic isolation module purification kit (E7490) (https://www.neb-online.de/en/art/E7490) and NEBNext Ultra II directional RNA library prep kit for Illumina (E7760) (https://www.neb-online.de/en/art/E7770S) (New England Biolabs, USA). The quality of RNA was estimated using Bioanalyzer 2100 (Agilent Technologies, California, USA) prior to sequencing. The NEBNext Multiplex Oligos primer set for Illumina (Index Primers Set 2) (E7500S) (New England Biolabs, USA) was used for preparation. At all stages, the material was cleaned using AMPure XP beads (Beckman Coulter, USA).