title: GSM7809975: hTSC, undifferentiated, clone CT27, Input; Homo sapiens; ChIP-Seq
library_name: GSM7809975
library_construction_protocol: Cells were fixed with 1% PFA for 10 min. After quenching and cell lysis, chromatin was sonicated. ChIP-Seq libraries of TFs were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB # E7645) with two custom adaptors: Adapter-T and Adapter-C. Adapter-T with a 3′-T overhang was prepared by annealing (5′-CTA CAC GAC GCT CTT CCG ATC TT-3′) and (5′-AGA TCG GAA GAG CAC ACG TCT GAA-3′; 5′ phosphorylated). Adapter-C with a 3′-C overhang was prepared by annealing (5′- CTA CAC GAC GCT CTT CCG ATC TC-3′) and (5′-AGA TCG GAA GAG CAC ACG TCT GAA-3′; 5′ phosphorylated). Adapter-C was added to increase the ligation efficiency.