title: GSM7876021: BC1.IP; Homo sapiens; RIP-Seq
library_name: GSM7876021
library_construction_protocol: Total RNA was extracted from the cultured cells using TRIzol reagent (Invitrogen Corporation, CA, USA). Ribosomal RNA was depleted using the Ribo-Zero rRNA Removal Kit (Illumina, Inc., CA, USA). The RNA concentrations were measured using NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA) m5C methylated RNAs were immunoprecipitated using the GenSeqTM m5C RNA IP Kit (GenSeq Inc., China). Both non-immunoprecipated input samples and m5C immunoprecipitation samples were used for RNA-seq library generation with the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, Inc., USA). Library quality was evaluated by BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). The library was sequenced on an Illumina Hiseq sequencer to obtain 150 bp paired-end reads.