title: BCR repertoire sequencing for Germline identification : RLl17_IgM using PMBCs
design: RNA was isolated using the Qiagen RNeasy kit and quality was assessed on Agilent Bioanalyzer. Reverse transcription was performed using Clontech SMARTer cDNA  template switching, which incorporates a common 5 primer during the template switch for full length transcript amplification. Ig amplicon libraries compatible with Illumina sequencing were generated by successive PCRs using a set of RM-specific primers specific for the constant region
library_name: RLl17_IgM_Week53_PBMC