title: GSM7875046: HFD_LKO, female8 ATAC-seq; Mus musculus; ATAC-seq
library_name: GSM7875046
library_construction_protocol: Nuclei from 50 mg frozen liver were isolated following the Chang Lab protocol (Corces et al, 2017; PMID: 28846090) for ATACseq library construction. Genomic DNA was transposed using transposase, purified using DNA Clean & concentrator-5 kit (Zymo D4014), pre-amplified with i7 and i5 indexed primers. Additional cycles were performed. Each amplified DNA library was purified using AMPure XP Reagent to remove primer dimers and large > 1,000 bp DNA fragments. PE150