title: GSM7876163: progeny round 3 rep B; Homo sapiens; OTHER
library_name: GSM7876163
library_construction_protocol: DNA from cells and virus pellets was extracted using a published protocol (Chen et al. 2015, Cell). The viral and cell pellets resuspended in 300 µl and 6 ml of NK buffer, respectively, and DNA extraction was continued normally with volumes adjusted to the 300 µl start, and GlycoBlue™ (Thermo-Fisher, catalog number AM9516) was added at the stage of DNA precipitation. the region of interest (sgRNA casette or targeted genomic locus) was PCR amplified with addition of illumina adaptors, followed by SPRI bead size selection and purification of the product.