title: GSM8232211: Heart, control, replicate3; Mus musculus; RNA-Seq
library_name: GSM8232211
library_construction_protocol: RNA was extracted from tissues using TransZol Up Plus RNA Kit (Q41206, Transgen, China) 1. Fragment the mRNA into smaller pieces using divalent cations or enzymes such as RNase III. 2. Reverse transcribe RNA fragments into first-strand cDNA using random primers or oligo(dT) primers. 3. Synthesize the second cDNA strand by adding dNTPs, DNA polymerase, and RNase H. 4. Perform end repair to generate blunt ends, followed by A-tailing by adding an adenine nucleotide to the 3' end. 5. Ligating sequencing adapters to cDNA fragments for subsequent PCR amplification. 6. PCR amplify the cDNA fragments with attached adapters to increase the amount of material for sequencing.