title: GSM8094014: Synovial fluid control; Equus caballus; ncRNA-Seq
library_name: GSM8094014
library_construction_protocol: RNA was isolated using the Quick-RNA Miniprep Kit (Zymo Research Corporation) and analyzed using an Agilent 2100 Bioanalyzer and an RNA 6000 Pico kit (Agilent Technologies) to determine RNA concentration, purity, and integrity per manufacturer's instructions. Samples from the control horses and horses with PTOA were pooled and shipped according to company protocols to GENEWIZ, a division of Azenta Life Sciences, for small RNA-sequencing. Library preparation included ligation of sequencing adapters to 5' phosphate ends. The RNA fragments were then converted to cDNA libraries prior to sequencing to target small RNA products of 18-30bp.