title: GSM6459578: 1198_RPPControl; Mus musculus; RNA-Seq
library_name: GSM6459578
library_construction_protocol: 3 independent sgKdm6a RPP (numbers) and 3 independent sgControl RPP mice (numbers) were euthanized and lung tumors dissected and finely minced mechanically using a razor blade and then enzymatically digested with Mouse Tumor Dissociation Kit (Miltenyi Biotec, #130-096-730) following the manufacturer's instruction. For each sample, about 16,000 cells were loaded onto a 10× Genomics ChromiumTM instrument (10× Genomics) according to the manufacturer's instructions. The scRNA-seq libraries were processed using Chromium Next GEM Single Cell 5' Kit v2 kit (10× Genomics). Quality controls for amplified cDNA libraries and final sequencing libraries were performed using Bioanalyzer High Sensitivity DNA Kit (Agilent). The sequencing libraries for scRNAseq were normalized to 4 nM concentration and pooled. The pooled sequencing libraries were sequenced on Illumina NovaSeq S4 300 cycle platform