title: NovaSeq of soil metagenome
design: DNA extraction was performed with the FastDNA Spin kit for soil (MP Biomedicals) and were normalized to 0.3 ng.L-1 before PCR amplification. Primers 479F (5 CAGCMGCYGCNGTAANAC 3) and R888 (5 CCGYCAATTCMTTTRAGT 3) were used to amplify the V3-V4 regions of the 16S rRNA gene.  Libraries sequencing was performed on a 2x250 NovaSeq 6000 by Fasteris SA (Switzerland).
library_name: SU_KM_As006_RI_16S