title: GSM8232097: Mouse 2504, female, infected, 12 mo; Mus musculus; RNA-Seq
library_name: GSM8232097
library_construction_protocol: Liver slices previously flash-frozen and pulverized using liquid nitrogen were used for RNA and DNA isolation. Both nucleic acids were isolated from the same liver slice using Allprep Qiagen kit (QIAGEN) following manufacturer's instructions with addition of RNase inhibitor and DNAseI to the original RNA isolation protocol. Samples were quality checked prior submission to bulk RNAseq. A RIN above 7 was accepted for downstream library preparation and sequencing. Samples that did not reach the criteria for downstream analysis were purified using either RNA mini kit (Zymo) or DNA purification kit (Zymo) Total RNA previously isolated from livers were submitted to Novogene for bulk RNA sequencing. Samples were enriched for mRNA using polyA selection. Libraries were produced using directional mRNA preparation and submitted to Novaseq PE150, with about 20M depth per sample.