title: GSM8186547: M1 mouse heart_Ctrl rep2_RNA-seq; Mus musculus; RNA-Seq
library_name: GSM8186547
library_construction_protocol: Total RNAs were isolated using Trizol Reagent from H9C2 cardiomyocytes and tissue samples. Small RNAs (<200 nt) were initially purified using the mirVana miRNA Isolation Kit (Thermo Fisher Scientific). RNA libraries were constructed following the protocols provided by Illumina and MGISEQ. For Ribo-seq, libraries were constructed using NEBNext® Multiple Small RNA Library Prep Set for Illumina® (catalog no.E7300S, E7300L). Briefly, adapters were added to both ends of RFs, followed by reverse transcription and PCR amplification. The 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina NovaSeq6000 by Gene Denovo Biotechnology Co. (Guangzhou, China).