title: GSM8353447: ACHN-CRISPRi Clone 1, D12; Homo sapiens; OTHER
library_name: GSM8353447
library_construction_protocol: Genomic DNA was isolated using the MasterPure Complete DNA Purification kit (Lucigen). Sequencing libraries were generated through two sequential rounds of PCR using Herculase II Fusion DNA polymerase (Agilent). For the first round of PCR, 6.6 µg of genomic DNA was used in each 100 µl reaction. A total of 12 first-round PCR reactions were performed per replicate, with 18 cycles of amplification. For the second round of PCR, 5 µl of the pooled first-round PCR product and primers containing barcodes and Illumina sequencing adaptors were used, with 6 to 8 cycles of amplification.