title: Integration site library of transposon MAG K241R mutant in K562 cell line
design: Extracted genomic DNAs were sonicated into an average size of 500 bp, and fragments between 200 and 500 bp were selected on agarose gel. Selected fragments were then damage repaired, end blunted, 3'-A-tailed and ligated with Y-adapter, which was created by annealing Miseq common oligo with barcode adapter of the TE. Two round of PCR were performed: first with P5-1 primer and GSP primers, and then with the P5-2 nested primer and GSP2 primers combined with specific primer for TIR and P7 adapter.
library_name: NGS-TESiteMapping-K562-MAG_K241R