title: GSM6568116: KMS28BM cells, THZ1, bio rep 1; Homo sapiens; OTHER
library_name: GSM6568116
library_construction_protocol: Cells were crosslinked with 1% formaldehyde and nuclei were isolated by cell lysis using lysis buffer (10mM Tris-HCl, 10mM NaCl, 5mM EDTA, 0.5% NP 40) and then homogenized using a Dounce homogenizer. The experimental procedure and data analysis were carried out as previously described (See et al 2022). HindIII-HF was used as the primary restriction enzyme and DpnII was used as the secondary. All ligations and disgestions were overnight for 16-18 hours, and were verified by 1% agarose gel. 4C library was amplified by nested inverse PCR using Phusion DNA Polymerase and custom primer pairs specific to the promoter of PPP1R15B.