title: GSM6940598: h33 sRNA req1; Arabidopsis thaliana; miRNA-Seq
library_name: GSM6940598
library_construction_protocol: Total RNA was isolated from 18-day-old seedlings using TRI Reagent (MRC, TR118) according to the manufacturer's instructions. 20ug total RNA was resolved on a 15% urea-polyacrylamide gel (Urea-PAGE) and small RNAs of 18~40 nucleotides (nt) were recovered from excised gel pieces corresponding to RNAs of this size range. Small RNA libraries were constructed following instructions from the NEBNext Multiplex Small RNA Library Prep Set for Illumina (E7300).