title: GSM7670594: HCT116_TRIM28; Homo sapiens; ChIP-Seq
library_name: GSM7670594
library_construction_protocol: HCT116 cells per sample were cross-linked with 1% formaldehyde for 15 min, quenched with glycine, and subjected to two-step cell and nuclear lysis in the presence of protease inhibitors. Shearing of samples was optimized such that the final DNA length was approximately 500 bp using a Bioruptor sonifier (Diagenode, ucd-200), and was followed by incubation with H3K9me3 polyclonal antibody (Abcam, ab8898) or ARID1A monoclonal antibody (CST, 12354) or TIF1β monoclonal antibody (CST,4124T) or SETDB1 monoclonal antibody (ABclonal, A6145) overnight at 4 °C. Immunoprecipitation was performed with 50:50 mixes of Pierce™ Protein A/G Magnetic Beads (ThermoFisher Scientific, #26162). Following elution and reverse cross-linking, purification (TIANGEN, DP203). 10–20 ng DNA were used to make the ChIP-seq library as per instructions from Illumina's ChIP-Seq Sample Prep kit (Illumina, IP-102-1001).