title: GSM8006112: ChIP_STAT6_UTC_24h_rep1; Homo sapiens; ChIP-Seq
library_name: GSM8006112
library_construction_protocol: For ChIPseq, cells were fixed with 1% formaldehyde for 5 minutes, after quencing and cell lysis, chromatin was sonicated using a bioruptor pico (Diagenode). For CUT&RUN, cells were centrifuged for 3 min at 600 g at room temperature and then resuspended in wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, 1x Roche Complete Protease Inhibitor EDTA-free tablet per 50 mL). Up to 500,000 cells per condition were centrifuged again for 3 min at 600 g at room temperature and resuspended in wash buffer. Library fragments were amplified using 1× NEB next PCR master mix and 1.25 M of custom Nextera PCR primers as previously described, sing the following PCR conditions: 72 °C for 5 min; 98 °C for 30 s; and thermocycling at 98 °C for 10 s, 63 °C for 30 s and 72 °C for 1 min. The libraries were purified using a Qiagen PCR cleanup kit yielding a final library concentration of ∼30 nM in 20 µl.