title: GSM7902575: monocyte derived, replicate3; Mus musculus; RNA-Seq
library_name: GSM7902575
library_construction_protocol: Single cell suspensions from murine epidermis were stained for FACS as described above. Donor and host CD11b+MHCII+ cells were sorted into RPMI medium supplemented 2% FCS and counted manually. Cell concentrations were adjusted to 500-1200 cells/µl and loaded at 7,000-15,000 cells per chip position using the 10x Chromium Single cell 5' Library, Gel Bead and Multiplex Kit and Chip Kit (10X Genomics, V3 barcoding chemistry) according to manufacturer's instructions. All subsequent steps were performed following standard manufacturer's instructions. Purified libraries were analysed by an Illumina Hiseq X Ten sequencer with 150-bp paired-end reads.