title: GSM7668932: MRC5_VA cells, no UV, 9 h after release; Homo sapiens; RNA-Seq
library_name: GSM7668932
library_construction_protocol: Total RNA was harvested with Rneasy mini kit (Qiagen), 2.0 ug of total RNA per sample was used for the construction of sequencing libraries. Ribosome RNA was removed from the purified total RNA using Epicentre Ribo-Zero™ rRNA Removal Kit (Epicentre), and the remaining RNA was used for library preparation using TruSeq Stranded Total RNA Sample Prep Kit (Illumina). Libraries were quantified fluorometrically using Qubit® dsDNA HS Assay Kits (Thermo Fisher Scientific) on a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). All libraries (> 2 nM/μl) were sequenced on an Illumina Novaseq 6000 (PE150 run) according to the manufacturer's instruction to a depth of 220-340 million reads on average.