title: GSM6733558: Vg2/3/4 CD103positive 1; Homo sapiens; RNA-Seq
library_name: GSM6733558
library_construction_protocol: Biopsies were dissociated according to the Miltenyl Human Tumour Dissociation kit protocol, with the modification that the volume of enzyme R used was reduced to 20% of that specificed in the manufacturers instructions and FACS purified. For GN096 the single cell suspension was FACS sorted directly into Trizol and frozen and sent to Genewiz for RNA extraction. For GN097, GN099 the single cell suspensions were FACS sorted direclty into PBS and frozen immediately and sent to Genewiz for RNA extraction. For GN142 the single cell suspension was sorted directly into lysis buffer of the PicoPure kit (Thermofisher) and DNA extraction occured immediately. For samples GN096, GN097, GN099 the samples were processed by genewiz using an ultralow input RNAseq library prep. For sample GN142 the RNA was processed by the advanced sequencing facility at the Francis Crick institute using the Nugen Ovation UltraLow RNA-Seq System.