title: GSM8232423: MOV_Mimic; Mus musculus; RIP-Seq
library_name: GSM8232423
library_construction_protocol: Mouse plantaris muscle was homogenized by cryogenic pulverization, then samples were then UV (254 nm) cross-linked twice. mmiRNA-mRNA complexes were ligated together on-bead after Ago2 immunoprecipitation. Ligating a barcoded RNA adapter to the complexes. Ligation occurs on-bead with PEG8000 to enhance efficiency. Post ligation, samples undergo gel electrophoresis and transfer to isolate RNA, which is then reverse transcribed. Excess oligos are removed, and a second DNA adapter is added. Cleanup precedes qPCR for cycle optimization, followed by PCR amplification and size selection through agarose gel electrophoresis. eCLIP-seq