title: GSM8142058: RNA_CA1200_4hrauxin_L3_rep1 [HW14]; Caenorhabditis elegans; RNA-Seq
library_name: GSM8142058
library_construction_protocol: L3 larvae were collected and stored in Trizol (Invitrogen) at -70C. Total RNA were extracted through freeze-crack method. RNA was cleaned up using Qiagen RNeasy MinElute Cleanup kit. mRNAs were purified using Sera-Mag Oligo (dT) beads (Thermo Scientific), then fragmented using Ambion (AM8740), and cleaned using Qiagen RNAeasy minElute kit. RNA was reverse transcribed using Superscript III system (Invitrogen, 18080-051). Residual dNTP was removed using ethanol precipitation. The second strand was synthesized using DNA polymerase I (Invitrogen). dsDNA was purified using Qiagen DNA minElute kit. 30 ng of input DNA were ligated to Illumina TruSeq adapters and amplified by PCR. Library DNA between 250 and 600 bp was gel purified.