title: RNA-seq of Fusarium xylarioides IMI389563 in culture
design: Strain morphologies were verified and grown in GYM broth for 7 days at 25oC and 175 RPM. Mycelia were harvested and total RNA extracted using the standard protocol of the RNeasy Mini kit (Qiagen, Hilden, Germany). The RNA quality assessment (Qubit and Fragment Analyzer), library preparation and Next Generation Sequencing (60M paired-end reads per sample) were performed by Genewiz/ Azenta (Frankfurt, Germany). Fastq files were quality-trimmed using trimmomatic parameters "ILLUMINACLIP:$TRIMMOMATIC_DIR/adapters/TruSeq3-PE.fa:2:30:10 SLIDINGWINDOW:4:5 LEADING:5 TRAILING:5 MINLEN:25".
library_name: IMI389563_culture_4