title: GSM8281760: Hippocampus_Control_rep3; Mus musculus; RNA-Seq
library_name: GSM8281760
library_construction_protocol: Hippocampal cells were isolated using the Adult Brain Dissociation Kit (Miltenyi Biotec), with freshly dissected brain tissues enzymatically digested and suspended in 0.5% BSA-PSA. Tissues were washed in cold D-PBS, dissected, and treated with enzyme mixes in a gentleMACS C Tube. Post gentleMACS processing, samples were filtered through a 70 µm MACS SmartStrainer, washed, and centrifuged. The supernatant was removed, cells were treated with Debris Removal Solution, and the final cell suspension was prepared for analysis. scRNA-seq libraries were prepared according to the Single Cell 3' Reagent Kits v2 manual. Cells were encapsulated with reagents and beads containing Illumina linkers, 10X cell barcodes, UMIs, and poly dT on a microfluidic chip to form GEMs. After cell lysis within each GEM, mRNA was reverse transcribed to cDNA, tagged with a unique barcode. The pooled cDNA was then amplified and prepared for sequencing.