title: GSM8096610: hESCs transfected with siDGCR8 and siFLII, Rep2; Homo sapiens; RNA-Seq
library_name: GSM8096610
library_construction_protocol: TRIzol reagent (Invitrogen) was used to isolate total RNA according to the manufacturer's instructions. The RNA was reverse transcribed with superscript IV, followed by 3' DNA adaptor ligation using T4 RNA ligase (NEB). Libraries were amplified with Q5 2× Master Mix. Finally, the DNA above 175 bp in libraries was isolated by gel-purification.