title: GSM7670366: LAC_camS::camS_rep1; Staphylococcus aureus; RNA-Seq
library_name: GSM7670366
library_construction_protocol: RNA was extracted using a RNeasy Mini Kit with the following modifications. Cells were lysed by incubation with 100 mM TRIS-HCL with 800 ng/µl lysostaphin for 25 min at 37 °C. Subsequently, 700 µl RLT buffer containing β-mercaptoethanol were added and samples were transferred to lysing matrix B tubes and the samples were subjected to three bead beating steps (30 sec x 3; 1 min on ice in between). RNA was treated with turbo DNAse kit according to manufacturers instructions. Depletion of ribosomal RNA and library preparations were performed at the Genomics Core at the University of Colorado Anschutz Medical Campus using the Illumina Ribo-ZeroTm Plus rRNA depletion kit and the Zymo-Seq RiboFree total RNA library kit.