title: GSM8395285: HARP patient 5024, baseline; Homo sapiens; RNA-Seq
library_name: GSM8395285
library_construction_protocol: [Collection ]  Whole blood was collected from MI patients or controls and platelets subsequently isolated. [Isolation] Platelets were isolated and purified by incubation with microbeads to deplete leukocytes and red blood cells (EasySep™ Human CD45 Depletion Kit II and EasySep™ RBC Depletion Reagent). Isolated platelets were lysed in 500 uL of QIAzol Lysis Reagent (Qiagen) and stored at −80°C. Platelet RNA was isolated with Direct-zol RNA microspin columns (Zymo Research), and quality and quantity were determined with a Bioanalyzer 2100 (Agilent Technologies). Sequencing libraries were barcoded and prepared using the Clontech SMART-Seq HT with Nxt HT kit (Takara Bio USA), and libraries were sequenced paired-end on an Illumina NovaSeq 6000