title: GSM8352676: Allo15MCAR-NKT (week 2) spleen; Homo sapiens; RNA-Seq
library_name: GSM8352676
library_construction_protocol: Peritoneal cavity cells (the tumor site) harvested from experimental animals at week 4 were subjected to FACS sorting for isolating therapeutic cells including MCAR-T and Allo15MCAR-NKT cells. Cells isolated from 10 experimental mice were combined for each group. Cells were quantified using a Cell Countess II automated cell counter (Invitrogen/Thermo Fisher Scientific). A total of 10,000 cells from each experimental group were loaded on the Chromium platform (10X Genomics), and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1 and the Chromium Next GEM Chip G Single Cell Kit (10X Genomics), according to the manufacturer's instructions. Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina). Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina).