title: GSM7809142: No drug 4h 3; Mycobacterium tuberculosis; RNA-Seq
library_name: GSM7809142
library_construction_protocol: 5 ml of culture from the 24 hour time-points or 20 ml of culture from the 4 hour time-points was pelleted at 4 degrees, snap-frozen in liquid nitrogen, and stored at -80 until RNA extractions were performed. Pellets were resuspended in 1 ml Trizol or equivalent generic reagent, disrupted with a Fastprep 5G (MP Bio) using tubes pre-filled with 600 mg 100 um zirconium beads, molecular grade (OPS Diagnostics) for two cycles of 40 seconds each at 9 m/s. RNA was then extracted and purified with a Direct-Zol Kit (Zymo) including on-column DNase digestion. rRNA was depleted using a set of biotinylated oligos designed as described in DOI 10.1128/mBio.00010-20. Paired-end Illumina sequencing libraries were constructed as described in DOI 10.1128/mBio.02012-21