title: GSM6635150: vehicle, early, replicate 2; Mus musculus; RNA-Seq
library_name: GSM6635150
library_construction_protocol: Total ribonucleic acid (RNA) was extracted using the RNeasy® Micro kit (Qiagen, Hilden, Germany) according to manufacturer's protocol. RNA was eluted in RNase free water and stored at -80°C. RNA concentrations were determined using the NanoDrop spectrophotometer.  For simultaneous isolation of total RNA and genomic DNA (and protein) for submission to RNA  sequencing and whole exome sequencing of CD5+CD19+ FACS-sorted cell the AllPrep DNA/RNA/Protein Mini Kit was used according to manufacturer's protocol. Library preparation of RNA was performed according to the Illumina TruSeq Stranded protocol on a HiSeq 2000 v4 sequencer, paired-end 125 base pairs with five samples per lane.