title: GSM8353126: NBProstate; Mus musculus; RNA-Seq
library_name: GSM8353126
library_construction_protocol: Neonatal mice were euthanized, the urogenital tract was removed and placed into cold PBS. After removal of the bladder and vas deferens and as much of the seminal vesicle and urethra as possible, the urogenital tissues with prostate buds were sectioned under a stereomicroscope, followed by enzymatic dissociation with 0.5 mL of 1% Trypsin for 30 min at 4°C. The dissociated tissues were washed with DMEM twice, and further dissociated by 0.1% collagenase B, with 1% DNase for 15 min at 37°C. After quenched by DMEM with 10% FBS, the urogenital tract cells were passed through a 45 μm filter. Library was performed according to the manufacter's instructions (Single cell 3' v3.1 protocol, 10x Genomics)