title: GSM7670251: NCD_1; Mus musculus; RNA-Seq
library_name: GSM7670251
library_construction_protocol: The isolated islet are quickly put into liquid nitrogen and stored at low temperature. TRIzol (Invitrogen, Waltham, Massachusetts, USA) was used to isolate total RNA from macrophages of isletr according to the manufacturer's protocol. The amount of total RNA was measured with Nanodrop 2000 (Thermo Scientific, Waltham, Massachusetts, USA), and the quality was evaluated by 1% agarose gel electrophoresis, and stained with 4S Red Plus nucleic acid stain (Sango, Shanghai, China). RNA_seq libraries were prepared using the KAPA Stranded RNA_Seq Library Preparation Kit.