title: GSM8096814: PC3-Replicate 2 normoxia (control for 0%O2); Homo sapiens; miRNA-Seq
library_name: GSM8096814
library_construction_protocol: Total RNA was isolated using miRNeasy MiniKit, according to the information given for each sample. Small RNA preparation for sequencing was performed according to Illumina TruSeq Small RNA library protocols. Briefly, indexed 3'- and 5'end RNA adapters were ligated to 1 ug of isolated total RNA per sample followed by reverse transcription and library amplification. The cDNA was purified on a PAGE gel by cutting out bands corresponding to 140-160 bp (length of miRNA + adapter and index sequences), pooled and ethanol precipitated.