title: GSM8094018: 293T, Scramble, rep3; Homo sapiens; RNA-Seq
library_name: GSM8094018
library_construction_protocol: Total RNA from cells was extracted using TRIzol reagent (Invitrogen), 1 ug of total RNA was used for the construction of  sequencing library. Total RNAs were processed with RiboMinus kit (Human/Mouse Module, Invitrogen) to deplete most ribosomal RNAs. Then, the ribo– RNA libraries for sequencing were prepared with illumina TruSeq® Stranded Total RNA Library Prep Gold (20020598) kit. These libraries were sequenced with X-ten at GENEWIZ, Suzhou, China. FastQC was applied for raw read qualities assessment.