title: GSM8282555: HiC, CEW1, early stage synchronized larvae, rep 1; Oscheius tipulae; Hi-C
library_name: GSM8282555
library_construction_protocol: Larvae were resuspended in PBS with 1 mM PMSF. A mortar and pestle (BioSpec Products, catalog # 206) was placed on dry ice and filled with liquid nitrogen. Drops of larvae were pipetted into the mortar, and pulverized into a fine powder while submerged in liquid nitrogen. Powdered larvae were crosslinked as described in the Arima Hi-C+ High Coverage Kit (A101030), and Hi-C was performed as per the manufacturer's instructions. Library preparation was performed with the KAPA Hyper Prep Kit (KK8502) as per Arima's instructions.