title: GSM8281409: PA0806.1-2-OE; Pseudomonas aeruginosa PAO1; RNA-Seq
library_name: GSM8281409
library_construction_protocol: The Pseudomonas aeruginosa PAO1 WT strain and the PA sRNA over expression strains were inoculated into LB with corresponding antibiotics overnight cultured at 37 °C. Then the strains were one hundred times diluted into fresh LB. Before harvested, the P. aeruginosa strains were cultured for 3 h until OD600 to 0.6, at 37 °C with 220 rpm shaking. Then, 2 ml of bacterial cultures were collected by centrifugation (12,000 rpm, 4 °C). RNA extraction was performed by using the TianGen RNA extraction kit with the recommended DNase digestion. After removing rRNA by following the RiboRid protocol, mRNA was used to construct the cDNA library according to the NEBNext Ultra II RNA Library Prep Kit protocol (NEB), which was then sequenced using the HiSeq 2000 system (Illumina). NEBNext UltraTM II RNA Library Prep Kit protocol