title: GSM6568213: ESC ad replicate1; Mus musculus; ATAC-seq
library_name: GSM6568213
library_construction_protocol: A total of 50,000 cells were washed with 50 μL of cold PBS and then nuclei were isolated in 50 μL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.2% (v/v) IGEPAL CA-630). Nuclei were then centrifuged for 10min at 800g at 4°C, followed by the addition of 50 μL transposition reaction mix (25 μL TD buffer, 2.5 μL Tn5 transposase and 22.5 μL ddH2O) using reagents from the Nextera DNA libraryPreparation Kit (Illumina #FC-121-103). Samples were then incubated at 37°C for 30min. DNA was isolated using a ZYMO Kit (D4014). ATAC-seq libraries were prepared using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541)