title: GSM7668912: MRC5_VA cells, no UV, even probe, ChIRP; Homo sapiens; ChIP-Seq
library_name: GSM7668912
library_construction_protocol: Cells were harvested by trypsin-treatment and fixed with 1% formaldehyde for 10 min. After quenching and cell lysis, nuclear lysate was sheared by sonication, fragmented chromatin was extracted. 2 ng of ChIRPed DNA and input samples were used for standard ChIP-Seq library preparation by using NEBNext Ultra DNA Library prep kit (NEB, E7103) and sequenced under Illumina NovaSeq 6000 system, resulting in the production of 150 bp paired-end reads.