title: GSM8186528: Adjacent normal tissue5; Homo sapiens; RNA-Seq
library_name: GSM8186528
library_construction_protocol: Total RNA was extracted from tissue samples by TRIzol reangent, and the concentration and purity of the extracted RNA was examined using Nanodrop 2000. 5ug of total RNA was used for construction of sequnencing libraries. RNA libraries for RNA-Seq were prepared using TruSeqTM Stranded Total RNA Library Prep Kit following manufacturer's protocols after removing rRNA by Ribo-Zero Magnetic Kit and mRNA by RNase R.