title: RNA-Seq of stem Capsicum annuum: Response 1h UV-B+Cold, rep2.
design: Total RNA was extracted from stem bell pepper at different times (1, 3 and 25 h) after exposure  to UV-B radiation+Cold using Trizol reagent (Ambion, Life Technologies, U.S.A.) according to the manufacturers intructioms with the following modifications: for precipitation step, we replaced 0.5 mL of isopropyl alcohol, with a mixture of 0.25 mL of isopropyl alcohol and 0.25 mL of 7.5 M lithium chloride; finally, RNA washes with 75% ethyl alcohol were carried out twice. Genomic DNA was removed with Turbo DNA free kit (Invitrogen, Life Technologies, U.S.A.). RNA was extracted from two biological samples comprising 10 bell pepper plants. RNA of acceptable purity and integrity (A260/A280:  1.8; RIN  8) was used to prepare cDNA libraries of 150 paired- end readings in the Illumina TruSeq library system. Libraries were sequenced using the Illumina NextSeq-500.
library_name: TRT1B