title: RNA-Seq of Noccaea caerulescens Bergenbach: Nickel, leaves, replicate 2
design: RNA were extracted with Qiagen RNeasy kit and treated with DNAseI. Libraries were constructed using 1 g total RNA with Illumina TruSeq Stranded mRNA kit by the POPS transcriptomic plateform (IPS2, Orsay, France) following Illumina kit recommendations. Library were sequenced on Illumina HiSeq2000 100 pb paired-end mode. Adaptors and low quality pair-end sequences were removed from the raw reads and the ribosomal RNA was filtered using the SortMeRNA algorithm (Kopylova, E., No, L. & Touzet, H.  (2012) SortMeRNA: Fast and accurate filtering of ribosomal RNAs in metatranscriptomic data. Bioinformatics 28, 32113217).
library_name: Ncbe_Ni_F_S2