title: GSM8281097: DNA, F244 cells; Mus musculus; OTHER
library_name: GSM8281097
library_construction_protocol: DNA and RNA were isolated from in vitro cultured 1956 and F244 cells using QIAamp DNA Kits (Qiagen, Cat# 56304) and RNeasy Kits (Qiagen, Cat# 74104), respectively, according to manufacturer's instructions. DNA was isolated from tails of female C57BL/6N and male 129S6 mice to serve as control for 1956 and F244, respectively. Exome sequencing libraries were generated using the NEBNext Ultra II FS Kit (NEB, Cat# E7805). Tumor RNA was subjected to DNase treatment and ribodepletion prior to library construction using NEBNext Ultra II Directional RNA library prep kit for Illumina (NEB, Cat# E7760).