title: GSM6456095: Subj_2 ULLS [S13]; Homo sapiens; RNA-Seq
library_name: GSM6456095
library_construction_protocol: In each participant, a vastus lateralis muscle biopsy (∼150 mg) was collected using a Weil–Blakesley conchotome at ∼2 cm from the motor point. Two ml of lidocaine (2%) were injected in the area of the sampling and a small incision of muscle and fascia was performed. Muscle samples were frozen in liquid nitrogen and stored at −80°C. RNA was extracted from the muscle tissues by REPROCELL (USA) using a Norgen Animal Tissue RNA Purification Kit #257 (Norgen Biotek Corp, Ontario, CA). RNA-Seq was performed at the Frederick National Laboratory for Cancer Research Sequencing Facility, NIH.  The libraries were made using the TruSeq Stranded Total RNA Library Prep protocol from Illumina.