title: GSM8239261: Vehicle 2; Staphylococcus aureus; Tn-Seq
library_name: GSM8239261
library_construction_protocol: genomic DNA (gDNA) from S. aureus cultures was extracted using the DNeasy Blood and Tissue Kit (QIAGEN). All DNA samples were then normalized to 100 ng/mL in ultra-pure water. Samples were then processed using the DTR gel filtration cartridges (Edge Biosystems Inc 42453) using the manufacturer's recommendations. The sample (50 mL) was sheared in Covaris tubes using the Covaris LE220 instrument to generate 350bp fragments. Sheared DNA was treated with terminal deoxytransferase to generate a 3' poly C-tail sequence, and two rounds of nested PCR were employed to amplify transposon junction regions. These products were multiplexed using 8-bp indexing primers and sequenced on the Illumina Hi-Seq 2500 at Tufts University Core Facility. Transposon sequencing