title: DNA seq of newly acquired spacer for DF60-TAdm strain CRISPR-Cas after virus treatment
design: The newly acquired spacer bands from PCR-amplified TAdm were recovered using the GeneJET Gel Extraction Kit (Thermo Scientific: K0691). A total amount of 1.5 g PCR products per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA, E7370L) following manufacturers recommendations and indexes were added to attribute sequences to each sample.
library_name: FDDP220313708-1a