title: GSM6568259: Tcell_d10_post_expansion_GFP-positive_GEM-well1_GEX; Homo sapiens; RNA-Seq
library_name: GSM6568259
library_construction_protocol: Primary T cells isolated directly from patient blood samples or peptide stimulated donor PBMC were stained with a mixture of pMHC displayed ENTER viruses. After 2 hr, cells were also stained with barcoded antibodies CD45RA, CD45RO, and IL7R (Biolegend totalseq-C, cat# 304163, cat# 304259, cat# 351356), live dead dye, CD3-APC and CD8-BV711 for 20 min on ice. Sample from each donor is also stained with unique hash-tag barcoded antibody (Biolegend totalseq-C, cat# 394661, cat# 394663). After two washes, CD8+ CD3+ GFP+ cells were sorted to load on two GEM wells of 10x genomics platform and CD8+ CD3+ GFP- cells were loaded on one GEM well, all using 5' RNA and VDJ kits according to manufacturer's protocol Library was prepared using Chromium Single Cell 5ʹ RNA and VDJ kit (10x Genomics).