title: GSM6459551: IRF3 KO THP-1 cells, uninfected, replicate 2 [2A_Unif]; Homo sapiens; RNA-Seq
library_name: GSM6459551
library_construction_protocol: Cytoplasmic RNA was extracted using Cytoplasmic & Nuclear RNA Purification Kit (Norgen; Cat. 21000, 37400) according to manufacturer's instructions. Total RNA libraries were prepared, starting with 500 ng of total RNA, according to the Illumina Tru-Seq Stranded Total RNA-Seq protocol (1000000040499) with RiboZero depletion, using unique dual indexes. 150 base paired-end sequencing was performed on a NextSeq 2000 P3 chip, loading pooled libraries at a concentration of 1,000 pM (protocol 1000000109376).