title: Long-read DNA-seq of Fusarium xylarioides IMI389563
design: High-quality high molecular weight genomic DNA was extracted from 1-2g of mycelium using the chloroform extraction method in [51] (full details in supplementary methods). 1 g of gDNA was diluted with nuclease-free water to a final volume of 47 l. Half of the DNA was then sheared into 20 kb fragments with a COVARIS g-TUBE (Covaris, Inc., Woburn, MA, USA), centrifuging at 4,200 rpm for 1 minute to increase DNA throughput. The shared DNA was converted into Oxford Nanopore libraries using the SQK-LSK110 library kit and the short fragment buffer (SFB) (Oxford Nanopore Technologies, UK). Library prep followed the standard manufacturers protocol apart from the final elution, which was done for 30 minutes at 37oC. The libraries were sequenced using a SpotON R9.4.1 FLO-MIN106 flowcell for 48 hours.
library_name: IMI_389563_longread