title: GSM8416047: ATAC_seq_WT, young, male, rep2; Mus musculus; ATAC-seq
library_name: GSM8416047
library_construction_protocol: Briefly, the process involves the following steps: Collect and lyse cells to obtain purified nuclei; Carry out the Tn5 enzyme transposition reaction to fragment the open regions of chromatin; Purify the fragmented DNA; Select target fragments to do the amplification. ATAC-seq was conducted following the protocol provided by 10K Genomics, Shanghai, China. Purified the above products using DNA Purification Kit (MN，740609). To determine PCR cycle number, take a small amount of the purified product and do a pre-amplification.After the pre-amplified bands were correct, then done formal amplification. After determining the yield and the band type, purified and constructed the library.Illumina NovaSeq 6000 sequencing platform was usually used, and PE150 sequencing mode was adopted.