title: GSM8142138: condDMSO_ctrl_reprep2_time0; Homo sapiens; RNA-Seq
library_name: GSM8142138
library_construction_protocol: RNA was extracted with the NucleoSpin RNA Plus kit (Macherey Nagel) following the manufacturer's protocol. Prior to rRNA depletion using the  NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat, E7400L), 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Libraries were equimolarly pooled and sequenced on a NextSeq550 system (Illumina) with 80 bases single-end.