title: GSM6454927: Liver, tissue resident memory, mouse C; Mus musculus; RNA-Seq
library_name: GSM6454927
library_construction_protocol: Infection protocol: IFNy-YFP reporter mice were infected with 5E5 CFU of Salmonella enterica serovar Typhimurium ΔaorA intravenously. Livers and spleens were collected at 45 days post infection sort protocol: CD4 T cells were enriched by MACs magnetic beads. Memory population was sorted with a BD FACS Aria and TRM were defined as CD69+ YFP+ CD4 T cells, TEM were defined as CD69- YFP+ CD4 T cells RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using QuantSeq FWD kit following manufacturers protocol