title: GSM6783962: GD131 spleen from pups with E.coli infection and with IL27RA KO, r3 [ATAC-seq]; Mus musculus; ATAC-seq
library_name: GSM6783962
library_construction_protocol: Cells were washed with cold PBS and pelleted by centrifugation (500g, 5min, 4°C). Nuclei were isolated by subjecting cells to 100uL of RSB-lysis buffer (10mM Tris-HCl ph 7.4, 10mM NaCl, 3mM MgCl2, 1uL/mL of Digitonin, 1uL/mL of Tween20, and 1uL/mL of NP40) for 3-5 min on ice. Libraries were prepared with approximately 50 to 75 thousand cells using a Tagment DNA Enzyme and Buffer Large Kit (Illumina, 20034198) and following the Omni-ATAC protocol (PMID: 28846090).