title: Cryopreserved  T3
design: Total RNA was extracted from potato tissues at its different stages using RNeasy Plant MiniKit (Qiagen, Venlo, Limburg, Netherlands). Quality of the RNA was checked on 1% agarosegel and estimated by NanoDrop (ThermoFisher Scientific, Wilmington, Delaware, USA).Sequencing libraries were generated with an average insert size of 200-bp read length usingIon Total RNA-Seq Kit v2 (Cat. No. 4476290) that changes RNA transcripts into cDNAlibraries. Dynabeads mRNA DIRECT Micro Kit (Cat. No. 61021) was used to preparepolyadenylated [poly(A)] mRNA (Ambion, Life Technology, Carlsbad, CA, USA). Thelibraries were checked and quantified using High Sensitivity DNA Assay kit on Agilent 2100Bioanalyzer System (Agilent, Santa Clara, CA, USA). Ion PI Hi-Q OT2 200 Kit (Cat.No. A26434) was used to prepare template-positive Ion PI Ion Sphere Particles on IonOneTouch 2 System. Ion PI Hi-Q Sequencing 200 Kit (Cat. No. A26433 and A26772)was used to sequence the libraries using Ion PI Chip Kit v3 on Ion Proton Systemfollowing manufacture&#39;s instruction (Thermo Fisher Scientific, Waltham, Massachusetts,USA)
library_name: IonXpressRNA 008