title: GSM8096366: S2-Exos-2; Homo sapiens; miRNA-Seq
library_name: GSM8096366
library_construction_protocol: Exosomes were resuspended in 500 uL of Trizol (Invitrogen, USA) for high-throughput miRNA sequencing (Cloud-Seq Biotech, Shanghai, China). Briefly, total RNA was extracted and the A260/A280 ratio measured using a NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA, USA). All samples had an A260/A280 ratio > 1.8. The small RNA libraries were constructed with GenSeq® Small RNA Library Prep Kit (GenSeq,Inc.) by following the manufacturer's instructions. Briefly, 3' and 5' adaptors were successively ligated to RNA samples. Then, the adaptor-ligated RNA was reversely transcribed into cDNA, followed by PCR amplification. After amplification, the cDNA libraries were size-selected for miRNA fractions before sequencing and then sequenced in a HiSeq platform (Illumina).