title: NextSeq 500 paired end sequencing; Expression profiling of type II skeletal muscle fibers from wild-type mice, myofiber-specific and satellite cell-specific Rrm2b knockout models
design: Expression profiling of type II skeletal muscle fibers from wild-type mice, myofiber-specific and satellite cell-specific Rrm2b knockout models
library_name: Sample 2_p
library_construction_protocol: Type II myofibers were collected from gastrocnemius muscle from each group of mice. The muscle tissue was directly frozen in liquid nitrogen for storage after collection. The muscle tissue was homogenized in the TriZol reagent, and RNA was precipitated by the isopropanol and ethanol. mRNA libraries were generated using the Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina).