title: GSM7093227: Dendritic cell, HTO-derived cDNA; Mus musculus; RNA-Seq
library_name: GSM7093227
library_construction_protocol: 20,000 live cells were loaded onto the BD Rhapsody cartridge, and cDNA trap and amplification were performed by the TAS-Seq method Cells were trapped and reverse-transcribed using an BD Rhapsody system (BD) according to the manufacturer's instructions, followed by the preparation of cDNA libraries and hashtag libraries by TAS-Seq method (Shichino et al. Commun. Biol,, 2022). The library preparation and sequencing analysis were conductd by ImmunoGeneTeqs, Inc (Chiba, Japan) by using Novaseq 6000 sequencer (Illumina, San Diego, CA, USA) and Novaseq S4 200 cycles v1.5 kit (Illumina).