title: GSM8239088: SEA06_emb_RNA_rep3; Caenorhabditis elegans; RNA-Seq
library_name: GSM8239088
library_construction_protocol: RNA-seq: Total RNA was purified following Trizol manufacturer's instructions after freeze-cracking samples five times. RNA was cleaned up using Qiagen RNeasy MinElute Cleanup kit. mRNAs were purified using Sera-Mag Oligo (dT) beads (Thermo Scientific) from 10 µg of total RNA. cDNA preparation was done in the presence of dUTP to prepare stranded RNA-seq libraries as in PMID:19620212, cDNA synthesis was performed in the presence of dUTP in order to prepare stranded mRNA-seq libraries. RNA-seq: cDNA was ligated to Illumina adapters and amplified by PCR. Library DNA between 250-500 bp in size was gel purified.