title: GSM6940258: WT_3; Mus musculus; RNA-Seq
library_name: GSM6940258
library_construction_protocol: Total RNA was extracted from freshly isolated islets and livers of Zzef1_KOand WT mice using RNeasy Micro kit (Qiagen), and sample qualities were assessed using an Agilent Bioanalyzer 2100 (Agilent technologies). 2 mg of total RNA samples of each group were then used. Sequencing libraries were constructed using the BGISEQ-500 platform (BGI, China). Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS). We included at least 3 independent biological replications.