title: DNBSEQ-G400 paired end sequencing; RNA-seq of Arabidopsis thaliana Col-0 seedlings treated with a Cutin Oligomeric Mixture (COM) able to elicit Pattern Triggered Immunity (PTI)
design: RNA-seq of Arabidopsis thaliana Col-0 seedlings treated with a Cutin Oligomeric Mixture (COM) able to elicit Pattern Triggered Immunity (PTI)
library_name: D3_p
library_construction_protocol: 14-day-old seedlings were treated 3 mg/mL of COM in 0.5 % DMSO in MiliQ water or the corresponding mock solution were added to each well. Seedlings were treated for 30 min, a total of two seedlings were collected and instantly frozen in liquid nitrogen. Arabidopsis thaliana Col-0 seedlings were germinated on solid MS media and after germination in liquid 1/2 MS media on an Aralab Fitoclima climate chamber with 150 μmol·s-1·m-2 light intensity, following a 10 h/14 h day/night cycle, under constant temperature of 20 °C and 60 % humidity.  Total RNA was extracted at 4 ºC from two ground seedlings  by addition of TRI reagent and chloroform, precipitated with isopropanol and washed with 70 % ethanol. Samples were subjected to DNase treatment using a TURBO DNA-free Kit (Ambion). Quantification and quality assessment of all RNA samples were evaluated on a TapeStation (Agilent). Libraries were generated at BGI according to the DNBSEQ stranded mRNA library system.