title: GSM7669863: AML_blood_time0_01_021; Homo sapiens; RNA-Seq
library_name: GSM7669863
library_construction_protocol: Blood samples were collected on day 1 of the trial prior to treatment with onvansertib and decitabine. RNA samples were collected in Paxgene tubes and total RNA extracted using the PAXgene blood RNA kit (Qiagen). Samples were pre-treated with ezDNase (ThermoFisher). cDNA synthesis used the SMART-Seq v.4 Ultra-Low Input RNA Kit (Takara Bio). NGS libraries were generated using the Nextera XT DNA Library Preparation kit (Illumina) and sequenced (paired-end 100bp) on a HiSeq4000 (Illumina).