title: GSM7669271: ATACseq_Ctrl_1; Homo sapiens; ATAC-seq
library_name: GSM7669271
library_construction_protocol: Sequencing library was generated using Omni-ATAC-seq protocol following recommendations and index codes were added to each sample for chromatin accessibility profiling. Briefly, the single cell nucleus suspension obtained above was incubated in 50 ul labeled Tn5 transposase mixture (25 ul 2×TD buffer, 2.5 ul transposase (100nM final), 16.5 ul PBS, 0.5 ul 1% digitonin, 0.5 ul 10% Tween-20, 5 ul H2O) for transposition reaction of each sample. Incubate reaction at 37ºC for 30 minutes. Cleanup reaction with a Zymo DNA Clean and Concentrator-5 Kit (cat #D4014). Pre-amplified transposed fragments were subjected to qPCR amplification. Purify the final PCR reaction using a Zymo DNA Clean and Concentrator-5 Kit (cat #D4014). Quantify library concentration using the KAPA Library Quantification Kit (cat #KK4854). Use 10× Geomics Chromium™ system to capture and mark each nucleus for the ATAC library construction. After ATAC library construction, Illumina high-throughput sequencing instrument was used to sequence samples and obtain sequencing data. ATAC libraries were sequenced using the Illumina NovaSeq 6000 platform, paired-end 150.