title: GSM6387637: Wild-type (wt) hypoxia; Mus musculus; RNA-Seq
library_name: GSM6387637
library_construction_protocol: Lungs isolated from 12-week-old mice were washed in cold PBS then digested in 2mg/ml Collagenase Type II (Worthington Biochemical, LS004174) for 45 minutes at 37°C, pipette mixing every 10 minutes to dissociate the cells. 100µm and 70µm cell strainers (BD Falcon) were used to filter cells before a 5-minute wash in red blood cell lysis buffer (Qiagen). Cells were resuspended in 250µl culture media (10% Fetal Bovine Serum, DMEM High Glucose with Glutamine, Penicillin Streptomycin) and cell viability (>85%) was measured using the Automated Cell Counter (Thermo Fisher, AMQAF1000). Chromium Single Cell 3' Reagent Kit v2 controller (10x Genomics)