title: GSM7809122: S2,NHS,CCG-1423,rep1; Drosophila melanogaster; OTHER
library_name: GSM7809122
library_construction_protocol: Chromatin from 10 million S2R+ cells was isolated with NUN buffer and fragmented with Diagenode Bioruptor using the high throughput power setting for five cycles. The solubilized chromatin was flash-frozen and stored at −80°C. Before run-on reaction, an equal amount of solubilized chromatin from untreated NIH3T3 cells was spiked into each sample, (counted to account for 1 % of the total chromatin). Sample preparation for PRO-seq was conducted as previously described with slight modifications (Chu et al., 2018; Mahat et al., 2016a; Vihervaara et al., 2021)