title: GSM8239640: Tibial nerves of DPN patient 3; Homo sapiens; RNA-Seq
library_name: GSM8239640
library_construction_protocol: For single-cell RNA sequencing experiments, tibial nerves from donors (4 DPN patients, 3 TLA patients) were rapidly frozen on liquid nitrogen in the operating room and stored until tissue digestion. Single-cell suspensions were generated by enzyme digestion and subjected to fluorescenceactivated cell sorting (FACS) to exclude doublets, debris, and DAPI+ dead cells. Single-cell RNA sequencing of human injured nerves was carried out using ChromiumTM Single Cell 3' Solution (10X Genomics). Libraries were sequenced using an Illumina NovaSeq6000 platform (performed by CapitalBio Technology, Beijing). The human nerve datasets were integrated and then analyzed for clustering and t-SNE visualization using the Seurat 4.0 package.