title: GSM8143741: AF_d3_2; Mus musculus; RNA-Seq
library_name: GSM8143741
library_construction_protocol: Tissue was first cryo-pulverized using the CP02 CryoPrep automated dry pulverizer (Covaris). Trizol (Invitrogen) based phenol-chloroform extraction was then performed for RNA purification, followed by on-column DNase digestion (Qiagen). Ribosomal RNA was depleted and libraries were constructed using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina) using 100 ng of RNA input. IDT for Illumina UD indexes were ligated to the libraries