title: GSM6455754: Donor 322- 4W; Homo sapiens; RNA-Seq
library_name: GSM6455754
library_construction_protocol: RPE cells were dissociated from culture plates using 0.25% Trypsin and Takara's ICELL8 3'DE chip and reagents kit was used for single cell capture and RT-PCR cDNA samples were concentrated and purified using a DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) and purified using a 0.6X proportion of AMPure XP magnetic beads (Beckman Coulter, Brea, CA) according to manufacturer protocols. Library preparation was performed using a Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) according to the Takara's 3' DE chip and reagent kit instructions. Libraries were sequenced using a NovaSeq 6000 high-output flow cell generating 2 × 150-bp read lengths (GeneWiz).