title: GSM7209460: Control-PyMT replicate 1; Mus musculus; RNA-Seq
library_name: GSM7209460
library_construction_protocol: Tumor tissue was homogenized in liquid nitrogen and RNA isolated in TRIZOL. RNA was extracted using the RNeasy Mini Kit (QIAGEN catalog # 74104) according to instructions provided by the manufacturer. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA with RIN values of 3.3-9.6 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102) with 8 cycles of PCR.