title: Interferon-gamma 1b-Induced Gene Expression Alters Neutrophil Function in Patients with Chronic Granulomatous Disease
design: Interferon-gamma 1b-Induced Gene Expression Alters Neutrophil Function in Patients with Chronic Granulomatous Disease
library_name: CGD05 V4_p
library_construction_protocol: IFN-γ administration was initiated at the dose of 50 mcg/m2 given subcutaneously on the standard schedule of Monday, Wednesday, and Friday in the evening before bedtime.  Then, 10-12 hours after the 1st dose, and the 4th dose of IFN-γ additional blood samples were obtained.    Neutrophils were isolated from heparinized peripheral blood by Dextran sedimentation, Ficoll-Hypaque density centrifugation, and hypotonic lysis of red blood cells 36,37.   Neutrophils were quantitated manually in a hemocytometer in the presence of 3% paraformaldehyde. RNA from neutrophils was isolated by standard technique. RNA was used to prepare libraries using the Universal Plus mRNA-seq kit from Tecan Genomics (Redwood City, CA).