title: GSM6634816: SGC7901 cells,WT,biol rep 2; Homo sapiens; RNA-Seq
library_name: GSM6634816
library_construction_protocol: Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) , high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library. RNA fragments were reverse-transcribed to create the cDNA by SuperScript™ II Reverse Transcriptase.The average insert size for the final cDNA library was 300±50 bp.