title: GSM8239315: A619, replicate 1, scATAC-seq; Zea mays; ATAC-seq
library_name: GSM8239315
library_construction_protocol: Approximately 5 μL of nuclei were diluted 10 times, stained with DAPI (Sigma Cat. D9542), and then assessed for quality and density with a hemocytometer under a microscope. The original nuclei were diluted with DNB buffer to a final concentration of 3,200 nuclei per uL. Finally, 5 uL of nuclei (16,000 nuclei in total) were used as input for scATAC-seq library preparation. scATAC-seq libraries were generated using the Chromium scATAC v1.1 (Next GEM) kit from 10X Genomics (Cat# 1000175), following the manufacturer's instructions (10xGenomics, CG000209_Chromium_NextGEM_SingleCell_ATAC_ReagentKits_v1.1_UserGuide_RevE).