title: GSM8281631: ATAC-seq_SKBR3_01; Homo sapiens; ATAC-seq
library_name: GSM8281631
library_construction_protocol: After a wash step in cold cell wash buffer, outer membranes were disrupted in lysis buffer for 2 min on ice. After a centrifugation step at 1,500g for 10 min, pelleted nuclei were kept for the next step. In a 50-μl final volume, tagmentation was performed for 30 min at 37 °C, using the Nextera DNA library prep kit. After addition of SDS to 0.2% final concentration, DNA was purified on AMPure XP beads using a 2:1 ratio (v/v) of beads:tagmented DNA. Freshly eluted DNA was barcoded and amplified in 110-μl PCR volume to generate a library with the following PCR program: 65 °C, 5 min; 98 °C, 30 s; 11 cycles of (98 °C, 10 s ramping to 65 °C, 30 s), 4 °C hold. ATAC libraries were then pooled at equimolar concentration and were sequenced multiplexed on the Illumina NextSeq550 with 50-bp paired-ends