title: Bulk RNA sequencing data of rice root sections for Single-cell RNA-seq developmental stage annotation
design: Bulk RNA sequencing data of rice root sections for Single-cell RNA-seq developmental stage annotation
library_name: Whole-4_s
library_construction_protocol: Sections (root tip to end of lateral root cap: Meristem; end of lateral root cap to the start of root hair elongation: Elongation; 1mm each beyond the start of root hair elongation: Maturation1 and Maturation2) were harvested into 10 μL RNA-later (Ambion) in the lid of a 1.5 ml tube. Samples were frozen in liquid nitrogen and stored at −80C X.kitaake was used as wild type. Rice seeds were dehulled, sterilized with 50% bleach for 10 min, and rinsed four to five times with sterile water. All plant growth was in Yoshida's nutrient solution solidified with gellan gum (Gelzan, Caisson Inc).Growth boxes were kept at 4°C in the dark for 2-3 days until the seeds germinate, then transferred to a percival growth chamber set to 28°C, and consistant light. Rice seedlings were grown in the percival for 1-3 days before harvesting. Tissue samples were processed by grinding with a blue homogenization pestle. RNA was isolated using the Zymo MagBead RNA Isolation kit according to the manufacturer's protocol (Zymo). RNA was used as input into the Lexogen QuantSeq 3′ FWD RNA-Seq library preparation procedure according to the manufacturer's protocol, using the Unique Molecular Identifier (UMI) PCR add-on kit.