title: GSM8239222: K562 cells, 4EHP-knockdown, rep1; Homo sapiens; RNA-Seq
library_name: GSM8239222
library_construction_protocol: Total RNA was harvested using TRIzol (ThermoFisher Scientific) and RNeasy Mini Kit (QIAGEN). Poly(A)+ mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer's protocol. Sequencing was performed using NextSeq 500 Sequencer and NextSeq 500/550 High-Output v2 Kit (75 cycles, Illumina).