title: RNA-Seq of Homo sapiens: mCAT-HeLa cell
design: Total RNA was extracted from mCAT HeLa cells using an RNeasy kit (Qiagen). Messenger RNA was purified from total RNA with the use of a NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). The purified RNA was used to prepare a cDNA library with the use of a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs).
library_name: KO_plus_NC_3