title: GSM8239674: 8330_Control_multiome_ATAC; Homo sapiens; ATAC-seq
library_name: GSM8239674
library_construction_protocol: Multiomes libraries were generated following the 10X Genomics Multiome Guidelines (protocol vA.01). Nuclei isolation were performed following the demonstrated protocol “Nuclei Isolation from Complex Tissues for Single Cell Multiome ATAC + Gene Expression Sequencing” available on 10XGenomics.com (cat #CG000375 Rev C). Briefly, 6 cerebral organoids per condition, control CHD1L+/+ and CHD1L-/-, were washed in PBS 1X. Lysis were performed using 1X NP40 Lysis Buffer with mechanical dissocation of tissues. Samples were filtered through 70µm strained and centrifugated for 5mn at 4°C 500rcf. Pellet were re-suspended in PBS + 1% BSA + 1U/µl RNAse Inhibitor and incubated for 5mn. After a second centrifugation, pellet was re-suspended in 0.1X Lysis Buffer and incubated 5mn on ice. Wash buffer was added and samples were centrifugated before a final resuspension in Diluted Nuclei Buffer. 10,000 nuclei were sent for analysis. Multiomes libraries preparation were generated following the 10X Genomics Multiome Guidelines (protocol vA.01) and the protocol “Chromium Next Gem Single Cell Multiome ATAC + Gene expression User guide” (cat #CG000338 Rev E). Sequencing was performed by the GenomEast platform, a member of the 'France Génomique' consortium (ANR-10-INBS-0009)