title: GSM6568535: C/EBP, Input; Mus musculus; ChIP-Seq
library_name: GSM6568535
library_construction_protocol: CMs harvested from WT and C/EBP-β mice were crosslinked with 1% formaldehyde for 15 minutes, following glycerine incubation for 5 min at room temperature. Cells were washed with cold PBS twice, lysed, and sonicated to shear DNA. C/EBP-β and FoxO1 antibodies were used to immunoprecipitate  protein/chromatin complexes. Several washing steps were followed by protein digestion  using proteinase K. Reverse crosslinking was carried out at 65 °C. DNA was subsequently  purified. ChIPSeq library preparation was performed with TruSeq ChIP Library Preparation  Kit.