title: GSM7809922: 2_FT_SA215061_SFO_sd_rep1; Mus musculus; RNA-Seq
library_name: GSM7809922
library_construction_protocol: Animals were anaesthetized with isoflurane. Brains were extracted and dissected in NMDG-ACSF. Tissue was digested with papain in trehalose-HEPES-ACSFand triturated to generate single cell suspensions. For stimulus to cell-type mapping experiments, actinomycin-D was included in NMDG-ACSF and trehalose-HEPES-ACSF to block artificial induction of immediate early genes. Sequencing libraries were constructed following 10x Genomics 3' v3.1 standard protocols.