title: HiSeq X Ten sequencing; Small RNA sequencing of mitochondria and whole fraction of human umbilical vein endothelial cells treated with hydrogen peroxide
design: Small RNA sequencing of mitochondria and whole fraction of human umbilical vein endothelial cells treated with hydrogen peroxide
library_name: Sample 5_s
library_construction_protocol: HUVECs were treated with 100 µM H2O2 for 12 hr and 18 hr.Then the mitochondrial fraction of HUVECs were isolated and purified by sucrose gradient  ultra-centrifugation. Total RNA in the whole cell or mitochondrial fraction was extracted using TRIzol reagent according to the manufacturer's instructions. Nucleic acid library construction was performed using the small RNA Library Prep Kit (Illumina).