title: GSM7413242: frotal cortex, CSS_control, rep6 [MC_C2A3]; Mus musculus; RNA-Seq
library_name: GSM7413242
library_construction_protocol: At the end of experimental periods, mice were anaesthetised and brains rapidly removed into ice-cold PBS. One hemisphere was stored in RNAlater and then dissected to obtain FC. Frontal cortex tissue was analysed for 6 T2D and 6 age-matched control mice, and 8 CSS and 8 age-matched control mice. Total RNA extraction  was performed using the Maxwell RSC simplyRNA Tissue Kit (Promega Corporation) on the Maxwell® RSC instrument per manufacturer's instructions. RNA was eluted into nuclease‑free water, quantitated and RNA integrity (via RIN) assessed. Library preparation (1.5ug total RNA) was undertaken for whole transcriptome sequencing using the TruSeq® Stranded Total RNA Library Prep kit, with the Ribo Zero Gold rRNA depletion kit, both of which are available from llumina.