title: GSM8238458: cranial NCCs, control, E10.5; Mus musculus; RNA-Seq
library_name: GSM8238458
library_construction_protocol: We harvested embryos at E10.5. We isolated and transferred the BA1 and the trunk region to 1.5 ml tubes, respectively. Tissues were diggested with TrypLE at room temperature for 5 min to make single cell suspensions. Cells were washed with PBS twice. Single cell RNA libraries were prepared according to the manufacture's protocol by loading onto the Chromium Single Cell 3' v3 microfluidics chip.