title: GSM6455916: mB_WT_H3K9me3_eSPAN_rep2; Mus musculus; OTHER
library_name: GSM6455916
library_construction_protocol: eSPAN in mESC, mouse B cells and HeLa  cells were performed exactly as previously described (Li et al., 2020).GRO-seq (global run-on sequencing) was performed as previous described (Barbieri, E. et al., 2020). GRO-seq libraries were prepared and deep sequencing was performed by Columbia University Genome Center. For CUT&TAG, CUT&RUN and eSPAN libraries, the supernatants were purified with ChIP DNA concentrator columns and library PCR was performed using standard Illumina Nextera Dual Indexing primers. Samples were pooled and sequenced using paired-end sequencing on Illumina NextSeq 500 platforms at Columbia University Genome Center.