title: GSM7901978: GMCSF+TGFb+IL34+FICZ, replicate 1; Mus musculus; RNA-Seq
library_name: GSM7901978
library_construction_protocol: Up to 100,000 cells were FACS sorted into RLT lysis buffer (Qiagen) supplemented with 14mM ß-mercaptoethanol. Cells were vortexed immediately after being sorted to ensure cell lysis. RNA was extracted using the RNeasy Micro kit (Qiagen) as per manufacturer's instructions, with an additional DNA clean-up step using RNase-free DNase I (Qiagen) Sequencing was performed on a NovaSeq 600 System (Illumina) to yield an average of 30 million reads per sample