title: GSM7668970: IMH-IICD45, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_name: GSM7668970
library_construction_protocol: The abdominal aortas pooled from mice in each group were digested with an enzymatic solution containing 2U/mL LiberaseTM (Cat# 05401127001, Switzerland, Roche) and 2U/mL elastase (Cat# LS002279, America, Worthington) in Hanks' buffered saline solution (HBSS) for 1 hour at 37℃. The cell suspension was strained through a 40 μm filter and washed thrice with PBS. The single cells were resuspended in opti-MEM with 10% FBS. The cell suspensions were loaded on a 10x Genomics Chromium instrument to generate single-cell gel beads in emulsion (GEMs). Library was performed according to the manufacturer's instruction (single cell 3' v2 protocol, 10x Genomics). Briefly, living cells in the abdominal aorta were resuspended in the master mix and loaded together with partitioning oil and gel beads into the 10x Genomics microfluidics chip to generate the gel bead-in-emulsion (GEM) in the GemCode instrument according to the manufacturer's protocols, in which cell lysis and barcoded reversed transcription of RNA, then amplification, shearing, and 5′ adaptor and sample index attachment were performed.