title: GSM7412613: NCCLu_185; Homo sapiens; RNA-Seq
library_name: GSM7412613
library_construction_protocol: Single-cell isolation was performed from pleural effusion by density gradient centrifugation. Isolated cells were cultured in AR5 medium (5% FBS, 1 X GlutaMax, 1X ITS, 1% penicillin/streptomycin, 50 nM hydrocortisone, 1 mM Na-pyruvate, and 1ng/ml EGF in RPMI) at 37°C  incubator. Short-term cultured single cells were preserved in freezing media (10% DMSO + 90% FBS) under -140°C until generating RNA library. RNA libraries were prepared for sequencing using standard Illumina protocols.