title: GSM8394920: Eos_with Cond1_LTbR FRC; Mus musculus; RNA-Seq
library_name: GSM8394920
library_construction_protocol: Total RNA from eosinophils isolated from the co-culture was extracted using the Rneasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions Stranded mRNA Library construction procedure were used as per BGI guidelines which involved using Oligo dT Selection (mRNA enrichment) followed by RNA fragment and reverse transcription, end repair, add A and adaptor ligation, PCR amplification, Single strand separation and cyclization, DNA nanoball synthesis followed by sequencing on DNBSEQ (DNBSEQ Technology) platform