title: Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli
design: Application of TraDIS to define the core essential genome of Campylobacter jejuni and Campylobacter coli
library_name: Sample 8_s
library_construction_protocol: Transposon libraries were grown for 16 hours in MH broth under microaerophilic conditions.  DNA was extracted using Qiagen g20 tips and genomic buffer system. Nucleic acid was tagmented using Nextera XT tagmentation kit. PCRs were performed using the illumina P7 primer and a customised P5 primer that hybridized to the end of the transposon. DNA was quantified using Quant-IT ds DNA HS assay kit.