title: GSM6456017: Horse4_inoculated_4; Equus caballus; RNA-Seq
library_name: GSM6456017
library_construction_protocol: Snap-frozen liver biopsy specimens were used for RNA isolation and NGS. RNA was isolated from mechanically homogenized liver biopsy specimens using the Machery Nagel RNA extraction kit according to the manufacturer's instructions The RNA sequencing library was generated from 500 ng total RNA using Dynabeads mRNA DIRECT Micro Purification kit (Thermo Fisher Scientific, Waltham, MA) for mRNA purification, fol- lowed by NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs, Ipswich, MA), both according to the manufacturer's protocols. The libraries were treated with Illumina Free Adapter Blocking and sequenced on Illumina NovaSeq 6000 using the NovaSeq 6000 S1 reagent kit (100 cycles, paired-end run 2 x50 bp), with an average of 5 x 10^7 reads per RNA sample.