title: GSM7809139: HM, Shp-1 f/f + chemo, scRNA-seq; Mus musculus; RNA-Seq
library_name: GSM7809139
library_construction_protocol: We isolated single cells from the bone marrow of leukemic mice, which were generated by transfecting MLL-AF9 or Hoxa9-Meis1 fusion gene into mouse embryonic stem cells. We used 2% EDTA-PBS to flush out the bone marrow cells and lysed the red blood cells. We counted and assessed the cell viability by flow cytometry and adjusted the cell concentration to 1000 cells/ul. scRNA-seq libraries were constructed using a Chromium Single Cell 30 v2 Reagent Kit, according to the manufacturer's protocol.