title: GSM8186252: scrnaseq_O2_4W; Homo sapiens; RNA-Seq
library_name: GSM8186252
library_construction_protocol: SC-islet cell clusters were collected and dissociated in single cells as described before, quantified for viability using the Vi-Cell XR (Beckman Coulter), resuspended in PBS with 0.1% BSA at a concentration of ~1000 cells/μl and put on ice. Before proceeding, all samples were ensured to have > 90% viability to minimize dead cells carry over in sequencing. Single cell samples were then processed using the Chromium 10x genomics instrument, with a target cell number of 10,000. The scRNA-seq libraries were created using the Chromium Single Cell 3' V3 Library & Gel Bead Kit, following the manufacturer's protocol. Single-cell encapsulation and library preparation were carried out as per the manufacturer's guidelines. Libraries were then pooled, and sequenced on the Illumina NovaSeq platform. The single-cell RNA sequencing (scRNA-seq) or multiome libraries were prepared according to the manufacturer's specifications.