title: ITS Metagenomics of Piper longum spikes
design: The amplicons from each sample were purified with Ampure beads to remove unused primers and an additional 8 cycles of PCR was performed using Illumina barcoded adapters to prepare the sequencing libraries. Libraries were purified using Ampure beads and quantitated using Qubit dsDNA High Sensitivity assay kit. Sequencing was performed using Illumina Miseq with 2x300PE v3 sequencing kit.
library_name: ITS_Stage II_Rep 2-Fp