title: GSM8096696: M12-inferior; Mus musculus; RNA-Seq
library_name: GSM8096696
library_construction_protocol: Mouse retinas were dissected and dissociated as previously described (Fadl, et al. Mol Vis 2020). A 1mm punch of superior or inferior retina dissociate at each age was used as input for 10X Genomics Chromium controller using v2 (Chromium Next GEM Single Cell 3ʹ GEM, Library and Gel Bead Kit v2) chemistry. Approximately 10,000 live cells per sample were loaded into the Chromium chip following the Cell Suspension Volume Calculator Table to capture transcripts from approximately 6,000 cells. Libraries were performed according to the manufacturer's instructions (Single Cell 3' v2 protocol, 10x Genomics)