title: GSM7210887: Oocytes from primary follicles, DM, rep 2 [LCMRNA2]; Mus musculus; RNA-Seq
library_name: GSM7210887
library_construction_protocol: Arcturus® Paradise® Plus RNA Extraction and Isolation Kit (#KIT0312I Arcturus Bioscience Inc., Mountain View, CA, USA) was used for isolation of total cellular RNA from the microdissected cells on the LCM caps, according to the manufacturer's protocol. RNA from each cap was pooled for the same oocyte stage during extraction. Total cellular RNA from oocytes collected in vivo and in vitro was isolated using Picopure (#KIT0204 Arcturus Bioscience, Inc., Mountain View, CA, USA). The Ovation® PICO SL WTA System V2 RNA amplification system (#3312 NuGen, Inc., San Carlos, CA, USA) was used for conversion of isolated RNA from the LCM-derived samples to cDNA and linear amplification. Library preparation for microdissected oocytes was performed using an Illumina TruSeq RNA Access kit at BGI, according to the manufacturer's protocol. RNA sequencing was performed on an Illumina HiSeq platform with two lanes (5 gb per sample). Library preparation for collected oocytes in both groups was performed using the Smart-seq II method, and RNA sequencing was performed using the Illumina HiSeq platform with one lane (5 gb per sample).