title: Oxford Nanopore MinION long-read sequencing for Entamoeba marina SRT209 genome
design: E. marina trophozoites were grown in a total 50 of tubes (15 mL polystyrene non-treated culture tubes) and harvested by centrifugation at 400 g for 5 min. High molecular weight DNA was isolated and purified using the NucleoBond HMW DNA Kit (MACHEREY-NAGEL, Dren, Germany). The purity and quantity of DNA samples were estimated using a NanoDrop 1000 (Thermo Fisher Scientific) and Qubit dsDNA HS assay kit (Thermo Fisher Scientific), respectively. The purified DNA was used to prepare the library using Rapid Sequencing Kit (SQK-RAD004; Oxford Nanopore Technologies, Oxford, UK). Sequencing was performed on the MinION platform with R9.4.1 Flow Cell (Oxford Nanopore Technologies) with MinKNOW 3.6.5. Base calling was performed by Guppy 3.2.10 (Oxford Nanopore Technologies). Finally, a total 435,782 reads (7.3 Gbp) were obtained.
library_name: EmNanopore