title: GSM6733482: HeLa_siRNA1, bsseq; Homo sapiens; OTHER
library_name: GSM6733482
library_construction_protocol: Total mRNA of HeLa cells was extracted by the Ribo-Zero rRNA depletion kit. Then 1 μg of the mRNA were used for bisulfite treatment by EZ Methylation Kit (Zymo) following the protocols provided by the manufacturer. After bisulfite conversion, the purified RNA was used for library construction by NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB).