title: GSM6564438: N-K24; Homo sapiens; RNA-Seq
library_name: GSM6564438
library_construction_protocol: Total RNA was extracted using TRIzol Reagent (Invitrogen, USA), and RNA concentration was determined using NanoDrop (ND-1000). After removing the rRNAs, 2ug of total RNA was used for the preparation of the cDNA library with a KAPA-Stranded RNA-Seq Library Prep Kit (Illumina, USA).  The total RNA was processed by NEBNext® Poly(A) mRNA Magnetic Isolation Module (or rRNA removal by RiboZero Magnetic Gold Kit) to enrich mRNA, and the product RNA was used for construction Library, via KAPA Stranded RNA-Seq Library Prep Kit (Illumina). Sequencing libraries, denatured by 0.1 M NaOH to generate single-stranded DNA, as amplified in situ Illumina cBot (TruSeq SR Cluster Kit v3-cBot-HS (# GD-401-3001, Illumina)). The ends of the generated fragments was used to run 150 Cycles by the Illumina HiSeq 4000 Sequencer.