title: GSM8185766: RUNX1::RUNX1T1 group rep1; Mus musculus; RNA-Seq
library_name: GSM8185766
library_construction_protocol: To harvest bone marrow, place the euthanized mouse in a supine position and sterilize the skin using ethanol. The Ilium\femur\fibula bones were cut off, ground in cold complete buffer (PBS with 2% fetal bovine serum (FBS) and 10mmol/L EDTA), and filtered through a 40 mm strainer. Then, gradient centrifugation (400G, 5min) was carried out and resuspended. EasySep™ Mouse T Cell Isolation Kit (STEMCELL, Cat # 19851) was applied to T cell negative enrichment. The enriched T cells were stained with the antibody against mouse CD3 and DAPI. DAPI- mCherry- CD3+ cells were sorted in both groups by flow cytometry with a BD FACSAria™ III sorter (BD Biosciences) for the scRNA-seq library. Single-cell RNA-seq libraries were prepared using the 10 Chromium Single-cell 5' and VDJ library construction (10 Genomics), following the manufacturer's instructions. Purified libraries were subject to NovaSeq 6000 sequencer (Illumina) for 150-bp paired-end sequencing.