title: GSM7092984: AD.F1; Mus musculus; RNA-Seq
library_name: GSM7092984
library_construction_protocol: Total RNA was extracted from cryopulverized hippocampal tissue by PureLink™ RNA Mini Kit (Invitrogen, 012183025) according to the manufacturer's instructions following lysis with the provided lysis buffer. RNA cleaning and concentration was done using Zymo Research, RNA Clean & Concentrator™-5 kit (cat# R1015) following the manufacturer's protocol. Fluorometric quantification of RNA and RNA integrity analysis was carried out using RNA Biochip and Qubit RNA Fluorescence Dye (Invitrogen). cDNA libraries were generated using NEBNext® Ultra™ II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L). Ribosomal RNA was removed using NEBNext rRNA Depletion Kit (human, mouse, rat) (E #E6310X). Libraries were indexed using NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #644OS/L). Library prep generated cDNA was quantified and analyzed using Agilent DNA chip and Qubit DNA dye.