title: GSM8281213: Myc_control_NSC_CUTandTag rep1; Drosophila melanogaster; OTHER
library_name: GSM8281213
library_construction_protocol: For CUT&Tag carried out by using interphase and mitosis neural stem cells or HEK 293T cells, the cells were fixed with 1% formaldehyde for 7min and quenched by Glycine. In other experiments, the cells are not fixed. The cells were arrested by ConA beads, and the factor of interest are combined by the specific primary antibody, followed by secondary antibody and Tn5 transposase. CUT&Tag libraries were prepared using Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) complemented with TruePrep Index Kit V2 for Illumina (Vazyme). 1w-100w cells were used as starting materials. Libraries were amplified using 13-19 cycles on the thermocycler. Post-amplification libraries were size selected at 200-2000 bp in length using VAHTS DNA Clean Beads (Vazyme).