title: Hi-C for skin fibroblast primary cells derived from control and LOS bovine day 105 fetuses
design: Hi-C sequencing for fibroblast cells was conducted by CD Genomics. Information on library preparation and sequencing obtained from the company is as follows: Cells were cross-linked with 1% formaldehyde at room temperature and quenched with glycine. The cross-linked cells were subsequently lysed. Endogenous nucleases were inactivated with 0.3% SDS, then chromatin DNA were digested by MboI (NEB), marked with biotin-14-dCTP (Invitrogen), and ligated by T4 DNA ligase (NEB). After reversing cross-links, the ligated DNA was extracted through QIAamp DNA Mini Kit (Qiagen) according to manufacturers instructions. Purified DNA was sheared to 300-500 bp fragments and were further blunt-end repaired, A-tailed and adaptor-added, followed by purification and PCR amplification. Finally, equimolar pooling of libraries was performed based on QC values and sequenced on Novaseq6000 sequencer (Illumina) with a read length configuration of 150 PE for 1,000M PE reads per sample (1,000M in each direction).
library_name: HiC_LOS_#3