title: RNA-Seq of Rhipicephalus microplus: adult female
design: The DNA samples were prepared for targeted sequencing with the Quick-16S NGS Library Prep Kit, Primer Set V3-V4. The sequencing library was prepared using PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final library was sequenced on Illumina MiSeq with a v3 reagent kit (600 cycles). The sequencing was performed with >10% PhiX spike-in. Unique amplicon sequences were inferred from raw reads using the Dada2 pipeline. Chimeric sequences were also removed with the Dada2 pipeline. Taxonomy assignment was performed using Uclust from Qiime v.1.9.1.
library_name: zr2746_12V3V4