title: RNAseq of Trichomonas gallinae and Escherichia coli Co-culture
design: First, the ribosomal RNAs from both eukaryotes and prokaryotes were depleted from total RNA samples. The remained RNAs were fragmented into around 250~300 bp and then reverse-transcribed into double-stranded cDNAs which subsequently went through end repair, A tailing and adapter ligation. After fragments size selection and PCR amplification, the metatranscriptome library was ready for library QC and sequencing.
library_name: CC3.fq