title: GSM8094886: C8_Molm13_colony_rep2, CpG; Homo sapiens; OTHER
library_name: GSM8094886
library_construction_protocol: Individual colonies were manually plucked using a 20µL pipette tip into 100µL of media, centrifuged at 200xg for 5 mins, and then resuspended in 20µL of RLT PLUS buffer before storage at −80°C. Lysates from single colonies (HL-60: 7.5µL; MOLM-13 and MV-4-11: 2.5µL) were used to separate genomic DNA and mRNA by G&T separation and as previously described following 'scTEM-seq for low-input samples' [Hunt, K. V. et al. sTEM-seq: Single-cell analysis of transposable element methylation to link global epigenetic heterogeneity with transcriptional programs. Scientific Reports (2022)] [Hunt, K. V. et al.  Protocol for targeted analysis of transposable element methylation levels and transcriptome in single cells using scTEM-seq. Protocol Exchange  (2022)]. Single colony TEM-seq and parallel RNA-seq analysis was performed as described, with minor modifications. During single colony TEM-seq library preparation, the number of SINE Alu amplification cycles was reduced to 29. For RNA-seq analysis, 15 cycles of cDNA amplification were used. [Hunt, K. V. et al.  Protocol for targeted analysis of transposable element methylation levels and transcriptome in single cells using scTEM-seq. Protocol Exchange  (2022)]