title: GSM6634370: circRNA Muscle_CTR4; Homo sapiens; RNA-Seq
library_name: GSM6634370
library_construction_protocol: Tissue samples were homogenized in TRIzol reagent and RNA was isolated with the RNAspin Mini Columns (GE Healthcare) Prior to library preparation, 2 μg of RNA were treated with 20 U of RNase R for 1 h at 37°C, after which RNA was isolated using Direct-zol RNA kit following the manufacturer's instructions; the isolated RNA was polyadenylated using a poly(A) tailing kit and then selected using Oligo(dT)25 Dynabeads following the manufacturer's instructions.  From the supernatant, which contained the enriched circRNA pool, RNA was isolated using Direct-zol RNA kit following the manufacturer's instructions; using the entire material, sequencing libraries were prepared, omitting the rRNA depletion steps, using Illumina TruSeq Stranded Total RNA Library prep kit following the manufacturer's instructions.