title: long-read sequencing of the tprK gene
design: The DNA extracting from 14 primary syphilis samples and 14 secondary syphilis samples was directly used for PCR amplification of tprK. PCR was conducted with tprK-specific primers appended to 16-bp PacBio barcodes under the following conditions: 98C for 2 minutes followed by 35 cycles of 98C for 10 seconds, 62C for 15 seconds and 68C for 2 minutes with a final elongation at 68C for 5 minutes. The resulting tprK amplicon was purified using 0.6x volumes of AMPure XP beads. barcodes (Table S1) under the following conditions: 98C for 2 minutes followed by 35 cycles of 98C for 10 seconds, 62C for 15 seconds and 68C for 2 minutes with a final elongation at 68C for 5 minutes. The resulting tprK amplicon was purified using 0.30x volumes of AMPure XP beads ((Beckman Coulter, Inc., CA, USA).
library_name: S11