title: GSM8005465: WT 1; Homo sapiens; RNA-Seq
library_name: GSM8005465
library_construction_protocol: RNA was isolated from selected clones using the RNeasy Plus Mini kit (Qiagen Cat# 74134). RNA yield was measured by fluorometric quantitation using Qubit RNA Broad Range kit (ThermoFisher Cat# Q10210) and RNA integrity and purity were determined by automated electrophoresis (Agilent TapeStation system) RNA-seq libraries were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Cat# E7760) according to manufacturer protocol.