title: GSM7874807: MEFS, male, Sirt3 knock-out, rep1; Mus musculus; RNA-Seq
library_name: GSM7874807
library_construction_protocol: RNA was extracted using Qiagen RNEasy Mini kit. Sample QC, library preparation and sequencing were performed by external NGS service provider (Novogen) After RNA fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.