title: GSM8142157: 786-O_xenograft_BBOX1_RNA_Rep2; Homo sapiens; RNA-Seq
library_name: GSM8142157
library_construction_protocol: Total RNA from triplicates was extracted from homogenized xenograft tumors or in vitro cells grown in 6-well plates by using RNeasy® Mini Kit (QIAGEN, 74106) with on column DNase digestion (QIAGEN, 79256). A total amount of 1 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. PCR products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100 system. Library preparation, sequencing, and data processing were performed by Novogene as paired end 100bp reads.