title: RNA-Seq HCoV-OC43 HCT-8
design: Libraries for high-throughput genome sequencing (HTS) were prepared by Novogene (UK) Company Limited (www.novogene.com). Each library was constructed using a minimum of 200 ng of total RNA, or a higher quantity if available. The total RNA samples were depleted of ribosomal RNAs (rRNA) from both eukaryotes and prokaryotes. The remaining RNAs were fragmented into 250-300 bp fragments and reverse-transcribed into double-stranded cDNAs. This was followed by end repair, A tailing, and adapter ligation. After fragment size selection and PCR amplification, the metatranscriptome libraries were rigorously quality-checked, and sequencing was executed on the Illumina platform by Novogene Company Limited (UK). Please note that the sequencing was not strand-specific.
library_name: T13