title: GSM7670546: T5_INF_S; Homo sapiens; RNA-Seq
library_name: GSM7670546
library_construction_protocol: Total RNA was isolated using the RNeasy Micro Kit Plus (Qiagen, Hilden, GER). RNA concentrations and integrity were measured using an Agilent Bioanalyzer and only samples with a RIN-value > 9 were included for sequencing Libraries were prepared using Illumina TruSeq Stranded mRNA (Illumina, San Diego, CA, USA) for poly-A selected strand-specific sequencing according to the manufacturers instructions. Sequencing was performed using Illumina NovaSeq 6000 (Illumina) with 'NovaSeqXp' workflow in 'S4' mode flowcell which generated 150 bp paired-end reads with a mean sequencing depth of about 83 million reads per sample.