title: GSM7668832: HK408-Stiff; Homo sapiens; RNA-Seq
library_name: GSM7668832
library_construction_protocol: RNA was extracted using Rneasy micorkit. 2-5µg RNA were used for library construction. Libraries for RNA-Seq were prepared with TruSeq Stranded mRNA Library Prep Kit. The workflow consists of mRNA enrichment and fragmentation. Cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. Strand specificity is achieved by replacing dTTP with dUTP and followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. cDNA generation is followed by A-tailing, adaptor ligation, and PCR amplification. Different adaptors were used for multiplexing samples in one lane