title: GSM8239649: Skin, TRPV1 activation,  Chrm HTO; Mus musculus; RNA-Seq
library_name: GSM8239649
library_construction_protocol: Back skin from TRPV1hM3Dq and control mice (three mice per group) treated daily with a CNO intradermal injection for three consecutive days, were dissected, separated into the subcutis and dermal-epidermal fractions and then digested. The dermal-epidermal and subcutis fractions were stained separately with TotalSeq™ anti-mouse Hashtag antibodies to mark the cells' anatomical location. Thy1+ SCA1+ stromal cells (CD45−CD31− EpCAM−) were sorted. The dermal-epidermal and subcutis fractions from TRPV1 activated and control samples were pooled together and sorted using a BD FACSAriaII with a 100-μm nozzle into RPMI medium supplemented with 10% FBS. Single cells were quantified using a hemocytometer. Cell capture, cDNA library preparation, quality control, and sequencing was performed at UC Irvine Genomic High Throughput Facility. SingleCell 3'- 10x