title: GSM8096051: ChIP,4do,Col0,ChIP,Rep2; Arabidopsis thaliana; ChIP-Seq
library_name: GSM8096051
library_construction_protocol: Fifty 4-5 days-old seedlings were harvested, crosslinked with 4 % formaldehyde for 4 hours, and homogenized in chloroplast enrichment buffer (0.33 M Sorbitol, 30 mM HEPES-KOH (pH 7.5), 0.001% beta-mercaptoethanol) using a pestle. The homogenate was filtered through two layers of miracloth, and centrifuged at 1500 g for 5 minutes at 4ºC. The pelleted chloroplasts were resuspended in 1 ml of chloroplast enrichment buffer. Sequencing libraries were prepared as reported (Bowman, et al. 2013, BMC Genomics, 14:466).