title: 16S_Seq_of_active_layer
design: DNA from the V4 region of the 16S rRNA gene was amplified with the primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3) primers (Caporaso et al., 2012). Sequencing was performed commercially by Microbiome Insights (Microbiome Insights, Vancouver, BC, Canada), with an Illumina MiSeq using V3 chemistry (Illumina, San Diego, California, United States).
library_name: S158