title: GSM7093053: SEP3tet_mock_rep1_R1; Arabidopsis thaliana; ChIP-Seq
library_name: GSM7093053
library_construction_protocol: ~1g inflorescence were collected as starting material. Then, tissue fixation and nuclei isolation were done according to the published protocol (Kaufmann, K., Muiño, J.M., Østerås, M., Farinelli, L., Krajewski, P., and Angenent, G.C. (2010a). Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). Nat. Protoc. 5: 457–472.). Native SEP3 antibody and Dynabeads protein A (Thermo Fisher) were used for immunoprecipitation. ChIP-seq library preparation was done with the ThruPlex DNA-Seq kit following the kit manual.