title: 16S amplicon seq of tonsil tissue
design: Sequencing libraries were prepared according to Illumina 16S Metagenomic Sequencing Library protocols to amplify the V3 and V4 regions. The input gDNA (2 ng) was PCR-amplified using 5 reaction buffer, 1 mM of dNTP mix, 500 nM each of universal F/R PCR primer, and Herculase II fusion DNA polymerase (Agilent Technologies, Santa Clara, CA, USA). The parameters for the first PCR cycle were heat inactivation for 3 min at 95, followed by 25 cycles of 30 s at 95, 30 s at 55, and 30 s at 72, followed by a 5 min final extension at 72.
library_name: HL0003467589