title: GSM8281116: M12RPF_A1-RPI38_2nd; Mus musculus; OTHER
library_name: GSM8281116
library_construction_protocol: 160 cells were sorted and lysed on ice with 22 mM Tris-HCl (pH 7.4), 1% Triton X-100, 100 µg/mL cycloheximide, 165 mM NaCl, 16.5 mM MgCl2, 5.5 mM CaCl2, 2.2 U/µL RNase inhibitor. Exposed RNA was digested by 10500 U/mL micrococcal nuclease at 37°C for 30 min and then ribosome-protected footprints (RPFs) were generated. The extracted RPFs for subsequent small RNA library construction was performed including end repair, adapter ligation, cDNA synthesis and PCR amplification. The purified library was sequenced with Illumina NovaSeq 6000 platform.