title: NextSeq 500 paired end sequencing; ATAC-seq of mouse Embryonic Stem Cell (mESC) line-derived cells subjected or not to doxycycline (dox)-induced overexpression of transcription factors
design: ATAC-seq of mouse Embryonic Stem Cell (mESC) line-derived cells subjected or not to doxycycline (dox)-induced overexpression of transcription factors
library_name: i8TFs dox V+CD+_1_p
library_construction_protocol: Doxycycline-inducible ESC lines were generated using the inducible cassette exchange method, the constructs and plasmids described in Bergiers et al., 2018; Iacovino et al., 2011; Vargel et al., 2016. Through gene synthesis (GenScript) and cloning, p2lox-5TFs and p2lox-3TFs plasmids were generated. They were consequently transfected into A2.lox ESCs (kindly provided by Dr. Michael Kyba – University of Minnesota). ESCs were maintained, expanded and differentiated as described in Serina Secanechia et al., 2022 Cell sorting, culture and transcription factor (TF) overexpression were performed as described in Serina Secanechia et al., 2022 ATAC-seq library preparation and sequencing were performed as described in Buenrostro et al., 2015 and Serina Secanechia et al., 2022