title: RNA-seq data set of EGFR mutant human non-small cell lung cancer cell lines PC9, HCC827, HCC4006 after NF2 knockout, YAP1 or WWTR1 overexpression or osimertinib treatment
design: RNA-seq data set of EGFR mutant human non-small cell lung cancer cell lines PC9, HCC827, HCC4006 after NF2 knockout, YAP1 or WWTR1 overexpression or osimertinib treatment
library_name: HCC4006_EV_DMSO_48h_R2_p
library_construction_protocol: ~2 million cells per sample were lysed in 350uL of RLT and stored at -80 until subsequent extraction. RNA was extracted using the RNeasy 96 QIAcube HT Kit (Qiagen) with a DNAse digest as per manufactures recommendations. Libraries were prepared using mRNA Stranded library preparation kit (Illumina) and subsequently quantified by Qubit and KAPA library quantification kit, ROX low (Roche). Library sizes were also determined by bioanalyzer.