title: GSM7669089: HepG2_Ctrl_H3R26me2s; Homo sapiens; OTHER
library_name: GSM7669089
library_construction_protocol: Cells were harvested and incubated with ConA-beads, cells were binded to the beads, 200 μL antibody buffer was incubated with beads for 1h at room tempreature, then the transposome was added into the beads and incubated for 1h at room tempreature, tagmentation buffer was added to the beads for 1h at 37℃, the reaction was stopped by adding EDTA and proteinase K, cell total genomic DNA was extracetd by phenol-chloroform. DNA library was constructed by NEB Next HiFi 2×PCR Mix, the PCR products were purified by VAHTS DNA clean beads (Vazyme).