title: GSM8186572: pTh17 Cells, ATAC-seq, shClns1a, Rep 2; Mus musculus; ATAC-seq
library_name: GSM8186572
library_construction_protocol: Extraction was performed using ATAC-Seq Kit (ActiveMotif, Cat. No 53150). In brief, pTh17 cells were restimluated with plate-bound aCD3 (1ug/ml) and aCD28 (1ug/ml) for 24h followed by enrichment with FACS. Cells were washed once with ice-cold PBS followed by lysis with ice-cold ATAC lysis buffer to get cell nuclei. library construction was performed using ATAC-Seq Kit (ActiveMotif, Cat. No 53150). In brief, tagmentation buffer was added into cell nuclei and samples were inclubated at 37 ºC for 30 min. Tagmented DNA was purifed with DNA prurification reagents follow by PCR amplification to construct final DNA library.