title: GSM7876686: ABLab_RNA_seq_NABC708_MMS_40min_replicate2; Caulobacter vibrioides; RNA-Seq
library_name: GSM7876686
library_construction_protocol: For RNA extraction, harvested  cells were spun down, the cell pellets were snap frozen and stored at -80°C. RNA was then extracted from these cell pellets according to a previous study (Badrinarayanan et al, 2017). RNA was isolated using Trizol extraction and purified via the Direct-zol RNA MiniPrep (Zymo, Cat. no. R2052) kit. The extracted RNA was treated with DNAse in order to remove the presence of genomic DNA from the sample. Total RNA was then purified using the RNA Clean & Concentrator-25 (Zymo, Cat. no. R1018) kit. Integrity of the RNA was assessed using Bioanalyzer instrument. mRNA was isolated from the total sample using the RiboMinus kit (Thermo, Cat. no. K155004) and submitted for sequencing. NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® with sample prep beads (Cat no: E7765L)