title: WGS of Candida albicans: SC5314 CRISPR-competent wildtype a/alpha
design: -80C frozen stock was struck to solid YPD medium and incubated at 30C for 2 days. Cells from the densest part of the streak were then used to start an overnight culture in liquid YPD medium at 30C. The next day, ~10^8 cells were used to extract genomic DNA using the Zymogen Quick-DNA Fungal/Bacterial Miniprep Kit, after which they were dual-barcoded and sequenced with 2x150 paired-end reads on an Illumina NextSeq 2000. After sequencing the reads were demultiplexed, and Illumina adapters were trimmed.
library_name: 1244