title: GSM8281160: Yeast RNA, 218-L-14-D1690 (sup35-218), rep3; Saccharomyces cerevisiae; RNA-Seq
library_name: GSM8281160
library_construction_protocol: Total yeast RNA was isolated using the GeneJET RNA Purification Kit #K0731 (Thermo Scientific, USA) according to the manufacturer's instructions. RNA concentration and quality were evaluated using a NanoDrop Spectrophotometer (Thermo Scientific, USA). Preparation of libraries for sequencing was carried out using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB #E7765, MA, USA) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs Set 2) (NEB #E6442S, MA, USA).