title: GSM8232435: FgPH-1_input; Fusarium graminearum PH-1; ChIP-Seq
library_name: GSM8232435
library_construction_protocol: ChIP technique was performed as described previously. (Gu et al., 2022). First, the mycelia were fixed with 1% formaldehyde, and were lysed. Second, Chromatin was sheared into 500bp fragments by sonication. Third, the selective immunoprecipitation of protein–DNA complexes were obtained by utilizing anti-GFP antibody (ab290, Abcam, Cambridge, UK) and their conjugated protein A agarose beads (sc-2003, Santa Cruz, CA). Finally, the cross-linked protein–DNA complexes were reversed, and the immunoprecipitated DNA was extracted by phenol/chloroform method. Half of the extracted DNA was then subjected to high-throughput sequencing on the IlluminaHiseq4000 platform by BGI (Shenzhen, China).