title: GSM7669860: RNA-seq of K562 cell line: 25nM dasatinib resistant; Homo sapiens; RNA-Seq
library_name: GSM7669860
library_construction_protocol: Total RNA was extracted using the Phenol:Chloroform method, followed by ribosomal and non-coding RNA depletion. Sequencing libraries were prepared using the NEXTFlex Rapid Directional RNA Seq Library Prep Kit (Perkin Elmer, Inc., Waltham, MA), depleting ribosomal and non-coding RNA. Quality control of RNA was performed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), using the Bioanalyzer RNA 6000 Nano assay. Libraries were sequenced on the Illumina MiSeq instrument (Illumina Inc., San Diego, CA), using a 150 cycle to produce 2x75 bp paired end reads.