title: GSM7670503: 20764X8 WT_Th1_Secondary_Day7_biol_rep_1; Mus musculus; OTHER
library_name: GSM7670503
library_construction_protocol: DNA was harvested from FACS sorted cells using the Quick-DNA MicroPrep Kit (Zymo Research) 10-200ng were fragmented by sonication (Covaris).Unmethylated cytosines were converted to uracils and sequencing libraries were created using the NEBNext Enzymatic Methyl Seq kit (New England Biolabs) according the manufacturer's instructions. DNA libraries were sequenced using an Illumina NovaSeq 6000 system following the manufacturer's protocols.