title: GSM7669447: PHH Donor 3 72h plated Replicate 1 [GC131311]; Homo sapiens; RNA-Seq
library_name: GSM7669447
library_construction_protocol: We performed RTqPCR, using progeny from 3 wells of 96 well plates for RNA extraction using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich) according to the manufacturer's instructions with addition of DNAseI from the On-Column DNase I Digestion kit (Sigma-Aldrich). Library preparation was performed with the LexoGen Quant Seq. Kit. Libraries were sequenced on an Illumina HiSeq4000 sequencing system.