title: HiC-seq of Streptococcus suis: Streptococcus suis strain SC19
design: For Hi-C sequencing: 1109 crosslinked cells (1% formaldehyde) were lysed by lysozyme (Tebu Bio , 0.4 mg/mL final concentration) for 30 min at 37. SDS (0.5% final concentration) is added to the mix and incubated at 65 for 10 min. Then the mix was added in the digestion buffer (400U MboI, 10mM TrisHCl, pH 8.0, 5mM MgCl2 and 0.1% TritonX-100) and incubated at 37 overnight. Pellet the insoluble fraction and resuspend it with dH2O. Marking the DNA ends with biotin-14-dCTP (0.4mM) and Klenow polymerase (5U/L). The ligation is performed with T4 DNA ligase (20U, Invitrogen) and incubated at 16 for 4h, followed by incubation overnight at 65 in presence of 250 mg/ml proteinase K and 5 mM EDTA. Purified DNA by phenol extraction and then sonicated DNA to 400bp fragments. The marked DNA was captured by Dynabeads MyOneTM Streptavidin C1 (Thermofisher) and NEBNext UltraTM II DNA library Prep Kit for Illumina (NEB) was used to generate the sequencing library.
library_name: Streptococcus_sui_m1