title: GSM6940130: Hic_Nitrogen-repletion_C0_replicat2; Nannochloropsis oceanica strain IMET1; Hi-C
library_name: GSM6940130
library_construction_protocol: For Hi-C experiments in N. oceanica, about 2 g samples were ground to powder in liquid nitrogen for the Hi-C experiment. Chromatin extraction was performed as described previously method. The procedure for the Hi-C experiment, including chromatin digestion, labelling of DNA ends, DNA ligation, purification and fragmentation, was as described previously(cell for hi-c method). DNA libraries were constructed using an Illumina TruSeq DNA Sample Prep Kit and sequenced on an Illumina HiSeq 3000 system. Hi-C experiments and sequencing were performed with two biological replicates.  Hi-C read mapping and normalization. Hic libraries were prepared for sequencing using standard Illumina protocols