title: GSM8186509: B6_naïve CD8 T cells_3; Mus musculus; RNA-Seq
library_name: GSM8186509
library_construction_protocol: RNA was prepared from sorted cells using a PureLink RNA Mini Kit (ThermoFisher). mRNA quality was assessed using NanoDrop (ThermoFisher Scientific) and RNA ScreenTape Analysis on an Agilent 2200 TapeStation (Agilent); all 10 samples had RINe scores >9, indicating negligible RNA degradation. Universal Plus mRNA-Seq library preparation kit with NuQuant (Tecan) was used with an input of 200ng of total RNA to generate RNA-Seq libraries.