title: BCR repertoire sequencing of vaccinated Rhesus macaque : RWb17_Week53 using plasmablasts
design: RNA was extracted following cDNA synthesis. Individual samples were then amplified in three separate reactions each using a unique Forward primer that contains a 5 Illumina adaptor sequence; a 4-nt diversity randomized sequence; a 6 nt-Illumina barcode; and a 3 anchor sequence specific to unique regions in the VH1, VH3 and VH4 leader region. A common reverse primer was used for all reactions that is comprised of a 3 anchor sequence to the 5 end of the IgG constant region.
library_name: RWb17_Week53_Prot1_Plasmablast_replicate1