title: GSM8232164: shPGR2; Homo sapiens; RNA-Seq
library_name: GSM8232164
library_construction_protocol: Each type of trophoblast stem cells were collected to isolate total RNA using the TRIzol reagent (Enzyme,  Jiangsu, China) following the manufacturer's instructions. The quality of RNA was assessed using an Agilent 2100 Bioanalyzer. RNA-seq libraries were constructed by NovelBio Bio-Pharm Technology (Shanghai, China). Subsequently, samples were treated with the Illumina mRNA-Seq Sample Preparation Kit (containing 1 box, part # 1004814, and 1 bag, part # 1004815) according to the manufacturer's protocol (Cat # RS-930-1001). mRNA was isolated from 5 μg of total RNA using oligo-dT magnetic beads and fragmented into small pieces at 94°C with divalent cations. The fragmented RNA was then converted into first-strand cDNA using reverse transcriptase and random primers, followed by second-strand cDNA synthesis using DNA polymerase I and RNase. The resulting cDNA fragments underwent end repair, addition of a single A base, and adapter ligation. After purification and PCR enrichment, a final cDNA library was generated. Each RNA-seq library was sequenced with 100 bp paired-end reads using the Illumina Genome Analyzer IIx.