title: GSM7876352: 231-LM-2; Homo sapiens; RNA-Seq
library_name: GSM7876352
library_construction_protocol: RNA was extracted using TRIzol reagent (Invitrogen). 1.3 ug of total RNA was used for the construction of sequencing libraries. A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly,ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.