title: GSM8096764: Tumor biopsy 65; Homo sapiens; miRNA-Seq
library_name: GSM8096764
library_construction_protocol: Total RNA was isolated from a piece of each biopsy (10 slices x 20μm), using miRNeasy MiniKit (Qiagen, Hilden, Germany), according to the information given for each sample. All samples had an RNA integrity number (RIN) of 6 or above, as assessed by the Agilent 2100 Bioanalyzer (Agilent Technology, Santa Clara, CA, USA). Small RNA preparation for sequencing was performed according to Illumina TruSeq Small RNA library protocols. Briefly, indexed 3'- and 5'end RNA adapters were ligated to 1 ug of isolated total RNA per sample followed by reverse transcription and library amplification. The cDNA was purified on a PAGE gel by cutting out bands corresponding to 140-160 bp (length of miRNA + adapter and index sequences), pooled and ethanol precipitated.