title: GSM7903047: MAC-T cells, IL10Rα knockout, no infection, rep4 (KO_12); Bos taurus; RNA-Seq
library_name: GSM7903047
library_construction_protocol: Total RNA extraction was carried out using the RNeasy mini kit (Qiagen, Germany) from both uninfected and MAP-infected samples according to the manufacturer's protocol. The DNA traces were removed by DNase I treatment (Fermentas, Waltham, MA, USA). The concentration and purity of the RNA samples were determined at A260/280 nm ratios using the Cytation-5 Spectrophotometer. The integrity of the RNA samples was assessed using Bioanalyzer. The samples with higher than 7 RNA Integrity Number (RIN) were chosen for RNA Seq. RNA-seq, including library construction were outsourced to Genewiz, Azenta Life Sciences, US