title: GSM6568472: WS-15c-T1; Trametes pubescens; RNA-Seq
library_name: GSM6568472
library_construction_protocol: The moisture content of the cultures was adjusted to 60% with sterile H2O. The stationary cultures were inoculated with 4 ml of homogenized fungal mycelium from low-nitrogen asparagine medium (pH 4.5), supplemented with 1% glycerol and incubated at 25°C for 21 days. RNA was extracted using CsCl ultracentrifugation and checked using a fragment analyzer (Advanced Analytical Technologies). Purification of mRNA, synthesis of cDNA library and sequencing on the Illumina HiSeq 2500 platform were performed at the GenomeScan in the Netherlands, https://www.genomescan.nl/.