title: GSM7874650: Skmel ctr 3; Homo sapiens; RNA-Seq
library_name: GSM7874650
library_construction_protocol: Total RNA was extracted through a Trizol-Chloroform separation step. The acqueous phase was then loaded onto the Qiagen Rneasy Mini Kit (Cat #74106) columns and  the manufacturer's instructions were followed (bypassing the step of cell lysis) in order to finish the RNA extraction. RNA libraries were prepared for sequencing using the kits Illumina® Stranded mRNA Prep, Ligation (20040534) and IDT® for Illumina® RNA UD Indexes Set A, Ligation (20040553), following manufacturer´s instructions and 10 cycles of amplification.