title: GSM7670473: H93 healthy control8; human feces metagenome; OTHER
library_name: GSM7670473
library_construction_protocol: Total genomic DNA was extracted from fecal samples using the PowerSoil® DNA Isolation kit((Mo Bio Laboratories) according to manufacturer's instructions. The quality and quantity of the extracted DNA were examined using Qubit dsDNA HS Assay Kit on a Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and electrophoresis on a 1% agarose gel, respectively. Paired-end libraries (insert size, ~350 bp) were prepared using a VAHTS Universal Plus DNA Library Prep Kit for Illumina (Vazyme Biotech). The library was sequenced on an Illumina NovaSeq 6000 platform (Biomarker Technologies Co., Ltd., Beijing, China) using the 150-bp paired-end sequencing mode