title: GSM8239184: Mouse Skin, Unwounded, 1; Mus musculus; RNA-Seq
library_name: GSM8239184
library_construction_protocol: Unwounded and wounded skin samples were minced in nuclei lysis buffer. They were ground with a loose pestle 22 times, passed through a 200μm pluriStrainer, and further ground with a tight pestle 12 times. Isolated nuclei were incubated in additional nuclei lysis buffer and washed before quality assessment under a microscope and counting. Samples were used immediately afterward for single nuclei RNA sequencing library preparation. Library preparation was performed using the Single Cell 3' v3.1 protocol from 10X Genomics according to the manufacturer's instructions. Briefly, isolated nuclei were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM).  Nuclear RNA contained in the gems was retrotranscribed to cDNA, and subsequent cleanup and amplification steps were performed as described in the protocol.