title: GSM8281964: RGC, PSCL_intact, cell16; Mus musculus; RNA-Seq
library_name: GSM8281964
library_construction_protocol: The RGC-labeled retina was quickly dissected out from mice after cervical dislocation and incubated with 0.5 mg/ml papain at 37 °C for 30 minutes. The retina was then washed with Neurobasal-A medium and triturated into a single-cell suspension by pipetting. After centrifugation at 300g for 6 minutes at 4 °C, the retinal cells were resuspended in Neurobasal-A medium supplemented with 10% B27.Mouth-pipetting was employed to pick single-labeled RGCs manually. A small volume of the RGC suspensions was transferred through a series of 1% BSA-containing PBS drops until a single targeted RGC was isolated. This RGC was then transferred into 4.2 µl lysis buffer, as per the Smart-seq2 protocol, and stored at -80 °