title: Illumina 20k mate pair sequencing for Entamoeba marina SRT209 genome
design: E. marina trophozoites grown in 15 mL polystyrene non-treated culture tubes (CLS430157; Corning, AZ, USA) in the late logarithmic phase were harvested by centrifugation at 400 g for 5 min. Total genomic DNA was isolated and purified using the QIAGEN Blood & Cell Culture DNA Kit with Genomic-tip 100/G (Qiagen, Germany). The purity and quantity of DNA samples were estimated using a NanoDrop 1000 (Thermo Fisher Scientific, MA, US) and Qubit dsDNA HS assay kit (Thermo Fisher Scientific), respectively. The purified DNA was used to construct 8 kb and 20 kb long jump distance mate-pair genomic libraries and sequenced with illumina HiSeq 2000 platform (100MP; illumina, CA, USA) by Eurofins Genomics (Tokyo, Japan). Finally, the 8 kb mate-pair sequence produced a total 121.7 million reads (10.7 Gbp), and the 20 kb mate-pair sequence produced a total 65.2 million reads (5.8 Gbp).
library_name: EmDNA_20kbMP