title: GSM8282531: S20-12254-L9; Homo sapiens; OTHER
library_name: GSM8282531
library_construction_protocol: We followed manufacturer Visium protocol for each step. Visium CytAssist spatial gene expression workflow for formalin fixed & paraffin embedded (FFPE) were performed for sectioning, and RNA quality assessment (DV200 >= 30%). Tissue slides were deparaffinized, hematxylin & eosin (H&E) stained, imaged, and decross-linked. Probe hybridization should be immediately performed according to Visium Spatial Gene Expression for FFPE reagent kit (10X Genomics). These probe pairs hybridize to their gene target and are then ligated to one another. The single stranded ligation products are released from the tissue to a capture area on the Visium slide within the CytAssist instrument. Once ligation products were captured, the slides can be removed from the instrument. Probes were extended by the addition of UMI, spatial barcode and partial read. Library was constructed according to 10X Genomics Visium Spatial pipeline.