title: NF-κB-induced expression of T-bet suppresses the proliferation of malignant B cells and is associated with good prognosis in chronic lymphocytic leukemia
design: NF-κB-induced expression of T-bet suppresses the proliferation of malignant B cells and is associated with good prognosis in chronic lymphocytic leukemia
library_name: 827_p
library_construction_protocol: Mice were euthanized by increasing concentrations of carbon dioxide. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells. Single-cell suspensions from spleens were prepared using the GentleMacs system (Miltenyi Biotech, Bergisch Gladbach, Germany). Splenocytes were enriched in TCL1 cells using EasySep™ Mouse Pan-B Cell Isolation Kit (Stemcell Technologies, Vancouver, Canada) for further analyses, resulting in a purity above 97% of CD5+ CD19+ cells, or cryopreserved and thawed when necessary. RNA isolation was performed according to manufacturer's recommendations using QIAshredder and RNeasy Mini Kit (both Qiagen, Hilden, Germany).  For RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Kit.