title: GSM8006495: Tnaive NT IgG rep2; Homo sapiens; OTHER
library_name: GSM8006495
library_construction_protocol: Cells were fixed wih 0.1% formaldehyde for 1 minute. After quenching, nuclei were extracted and permeabilized using 0.01% digitonin. Chromatin was digested with Mnase and released via incubation at 37ºCfor 30 minutes. CUT&RUN libraries were prepared via the NEBNext Ultra II DNA Library Prep Kit for Illumina. Libraries were amplified for 15 cycles on a thermocycler and verified via agarose gel electrophoreses