title: RAD-sequencing of rare asexual Artemia males, their mothers and sisters.
design: protocol from Johnson et al. 2008 'Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers'. DNA was digested by PstI. P1-ligated fragments were sonicated, then size-selected by gel migration. P1 and P2-ligated fragments were amplified by 12 PCR cycles.
library_name: A1