title: GSM8415686: MDSC_hCD84_2; Homo sapiens; RNA-Seq
library_name: GSM8415686
library_construction_protocol: RNA from IVD-hMDSCs was extracted using RNeasy Micro Kit (Qiagen, Cat n=74004), following manufacturers' guidelines. RNA integrity was checked with Agilent 2100 Bioanalyzer system. RNA integrity was checked with Agilent 2100 Bioanalyzer system. NEBNext UltraExpress™ RNA Library Prep Kit (New England BioLabs Inc,) and NEBNext poly (A) mRNA magnetic isolation module (New England BioLabs Inc) were used for cDNA synthesis. The libraries were sequenced using the NextSeq 2000 and P2 kit High (100 cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.