title: NextSeq 500 sequencing; bulk RNA-seq of wild-type and mutant germinating Arabidopsis thaliana seeds
design: bulk RNA-seq of wild-type and mutant germinating Arabidopsis thaliana seeds
library_name: wrky3_2_s
library_construction_protocol: Homozygous mutant lines were then assessed for germination kinetic after 24 h in light . Seeds from two individual parent plants of each mutant line were included. Seeds were sterilised and stratified for 48 h of cold (4\degree) dark stratification before being transferred to continuous light (at 22\degree), then collected after 24 h in the light RNA extraction using the Spectrumâ„¢ Plant Total RNA Kit (Sigma) Libraries were prepared using TruSeq Stranded mRNA Library Prep kit