title: GSM8394142: CUTTL1 cells Actinomycin D 180min, DMSO, rep2; Homo sapiens; RNA-Seq
library_name: GSM8394142
library_construction_protocol: RNA was extracted using 'miRNeasy Mini' (217004, Qiagen Benelux BV, Venlo, The Netherlands) or 'miRNeasy Micro' (217084, Qiagen Benelux BV) kits, and DNA digestion was done on-column according to the manufacturer's protocol. RNA extracted as described above was further used for full length RNA sequencing utilizing the Watchmaker total mRNA library preparation kit (7BK0001-096, Watchmaker Genomics, Boulder, CO, USA) according to manufacturer's instructions). Prepared libraries were further sequenced using the AVITI sequencer (Element Biosciences, San Diego, USA) using the AVITI 2x150 High Output Sequencing Kit (860-00001, Element Biosciences, San Diego, USA).