title: Illumina MiSeq paired end sequencing; DNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
design: DNA-seq of E. coli BL21 (DE3) Evolved in High pH (pH = 9) Terrific Broth Media
library_name: SM2B_p
library_construction_protocol: Cultures of E. coli BL21 (DE3) - control and evolved - were harvested in stationary phase and 2 x 10^9 cells were collected for processing. DNA from E. coli BL21 (DE3) samples was extracted using the DNeasy Blood & Tissue Kit (Qiagen) according to the manufacturer's instructions for gram-negative bacteria. The sequencing library was constructed from the isolated genomic DNA using the NEB DNA Ultra II kit – dual indexing (New England Biolabs). The library was re-sequenced using a MiSeq Reagent Kit v2 (Illumina) in a 500 cycle (2 x 250 bp pair-end reads) reaction on the MiSeq instrument (Illumina).