title: GSM7876050: IMR32_siTPX2_si47_rep1; Homo sapiens; RNA-Seq
library_name: GSM7876050
library_construction_protocol: RNA extraction was performed practicing the manufacturer's instructions of miRNeasy kit (Qiagen) including on-column DNase treatment and nanodrop (Thermo Scientific) was used to determine the concentration. RNA quality was determined with the Experion automated electrophoresis system (BioRad) prior to profiling. The 3'UTR QuantSeq library kit from Lexogen was used, during library preparation 15 PCR cycles were used.  qPCR quantification of the libraries using the illumine Kapa library quantification kit (Lightcycler 480 qPCR mix Kapa. RNA-seq libraries were sequenced on the NextSeq 2000 platform (Illumina) using the NextSeq 1000/2000 P2 (100 cycles), single-end kit (Illumina).