title: GSM6634808: SGC7901 cells,△IDR,FLAG; Homo sapiens; ChIP-Seq
library_name: GSM6634808
library_construction_protocol: All tissue was harvested for the immunoprecipitation (IP) reaction. The tissue preparation and ChIP protocol closely adhered to the steps outlined in the SimpleChIP® Plus Sonication Chromatin IP System manual (Cell Signaling Technology, # 56383). A total amount of 50ng DNA per sample was used as input material for the ChIP sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample.The library preparations were sequenced on an Illumina platform and 2x150bp paired-end sequencing reads were generated.