title: Shotgun sequencing of Homo sapiens: composite urine
design: DNA was extracted using phenol chloroform method. A DNA qubit assay (Thermo Fisher Scientific) was used to measure DNA sample quantity, and Genomic DNA ScreenTape (Agilent) was used to check DNA integrity. The sequencing library was prepared using the KAPA HyperPrep Kit (Kapa Biosystems) following the manufacturers recommendation. In brief, 2x 10 -10 Kg of input DNA was end- repaired and 3&#39;-dA tailed after being sheared by the Covaris LE220 ultrasonicator. Adapter was then ligated to the DNA sample, and the ligated product was PCR amplified and cleaned up using SPRIselect Reagent (Beckman Coulter). Samples were pooled and sequenced on an Illumina HiSeq X sequencer for 150 bp read length in paired-end mode, with an intended output of 20 million reads per sample
library_name: 19203_0502