title: GSM6634803: whole body discarding last third from the cloaca, 23dph, rep2 [ATAC-seq]; Scophthalmus maximus; ATAC-seq
library_name: GSM6634803
library_construction_protocol: Turbots at different stages were collected to reach 75,000 cells per sample. 3dpf stage was treated with 1mg/ml pronase in 1X PBS to remove the chorion. Cells were lysated after incubation with 10mM Tris, 10mM NaCl, 3mM MgCl2, 0.1% NP40 for transposition reaction and PCR amplification. Transposition with Tn5 enzyme and PCR amplification