title: GSM8353072: H3 HAR Perturb-seq Gene Expression, Batch 1, Lane 1; Homo sapiens; OTHER
library_name: GSM8353072
library_construction_protocol: Cells were washed 2x in Dulbecco's PBS, then released with Accutase for 4 minutes. Cells were resuspended in ice cold DPBS with 0.4% Bovine Serum Albumin and placed on ice prior to single cell partitioning. Cells were encapsulated in gel beads in emulsion (GEMs) using a 10X Controller with 10X Chromium 3' v3.1 reagents, using the manufacturer's protocol. Library construction was performed according to the manufactuerer's protocol (Chromium s3' single cell v3.1 with Feature Barcoding for CRISPR, Dual Index; 10X Genomics). Two libraries are made per GEM lane (gene expression and CRISPR capture).