title: GSM6634580: Treg cells, Ctrl-rep1; Mus musculus; RNA-Seq
library_name: GSM6634580
library_construction_protocol: Treg cells were isolated from Foxp3-Mettl14f/+ cKO mice and littermate controls on day 7 post-transplantation. Total RNA was extracted with TRIzol and mRNA was then separated by using a Dynabeads mRNA Purification Kit (Invitrogen). Standard Illumina sequencing was applied for sequencing. Raw RNA-sequencing reads were aligned to the mouse genome (mm10) with TopHat. Genes were considered significantly differentially expressed   if the fold change was greater than 1.5-fold and less than 0.1. A GO enrichment analysis and KEGG pathways enrichment analysis were performed using online bioinformatics tools. RNA libraries were prepared for sequencing using standard Illumina protocols