title: Solanum dulcamara 39084 genome
design: Solanum dulcamara ID 39084 obtained from seeds provided by the Royal Botanical Gardens Kew (UK). Seeds were surface-sterilised in 1% bleach and rinsed and then stratified during 10 days on wet tissue paper mianteined at 4C and darkness. After that, they were moved to soil (John Innes No2) and kept in a growth room of the University of York with an average temperature of 20C (2C) and 14h/10h light /dark. Root, stems, leaves, flowers and berries were collected from each plant simultaneously and stored at -80C until further processing. The plant material was ground using liquid nitrogen. The RNA was extracted using the E.Z.N.A.  Plant RNA Kit (VWR) including the treatment with DNase to remove residual DNA according to the manufacturers instruction. The quality of the RNA was assessed with Nanodrop, Qubit 4.0. using the Qubit RNA Broad Range and Agilent Technology 2100 Bioanalyzer (Agilent Technologies, CA, USA). Only samples with RIN >6 were used to prepare the cDNA libraries.
library_name: Sd_39084_ONT_long_cDNA_annotation_berries