title: GSM8141813: CD45+cells, untreated_lung; Mus musculus; RNA-Seq
library_name: GSM8141813
library_construction_protocol: Mouse lung tissues were harvested and dissociated to single cells. Live CD45-positive immune cells were sorted  and resuspended at a concentration of 1 × 106 per ml in 1× PBS with 0.4% BSA for 10x genomics processing. Cell suspensions were then loaded onto a 10x Genomics Chromium instrument to generate single-cell gel beads in emulsion (GEM). Approximately 10,000 cells per sample were loaded. scRNA-seq libraries were prepared using the following Single Cell 3′ Reagent Kits: Chromium Single Cell 3′ Library and Gel Bead Kit v2 (PN-120237), Single Cell 3′ Chip Kit v2 (PN-120236), and i7 Multiplex Kit (PN-120262; 10x Genomics), as directed by the Single Cell 3′ Reagent Kits v2 User Guide (Manual Part # CG00052 Rev A). Libraries were run on an Illumina HiSeq 4000 system (SY-401-4001, Illumina) with 2 × 150 paired-end reads for approximately >90% sequencing saturation. cDNA integrity, libraries quality , number of cells sequenced, and mean number of reads per cell were confirmed before further processing.