title: GSM6568516: ET assay 4, replicate B; Homo sapiens; OTHER
library_construction_protocol: HEK293 RNA was extracted using Trizol. PolyA RNA was selected using Invitrogen Poly(A)Purist. Custom, RNA was reverse transcribe into cDNA using Invitrogen SSIV. Two rounds of PCR primers target constant flanking sequence of reporter assay transcript and incorporate sequences for sequencing by Illumina  sequencer platforms. Other: exon-trapping. Reverse transcription of reporter gene containing query DNA sequence and PCR targeting sequenc flanking the reporter query DNA sequence.