title: GSM8005862: RIBO-504; Mus musculus; OTHER
library_name: GSM8005862
library_construction_protocol: Total RNA was extracted from sampled human brains using miRNeasy Mini Kite and homogenized in Qiazol using stainless steel beads. Chloroform was then used for phase separation. RNA containing an aqueous layer was further purified using the RNeasy MinElute spin column. RNA was finally eluted in RNase-free water. RNA-seq libraries were prepared in parallel from the same lysate used to prepare RIBO-seq libraries. RIBO-seq and RNA-seq libraries were pooled and sequenced on a NovaSeq6000 sequencer, single read, 1x50 cycles, for a total of ≥ 130M reads/RIBO-seq and ≥ 50M reads/RNA-seq library. Library prep and sequencing were performed at TB-SEQ (Palo Alto, CA).