title: RNA-seq of NCF mus musculus with Spp infection for 24 hrs: adult male heart
design: The cardiac RNA-seq was carried out in the following groups of both normal chow and HFD fed mice: control (prior to viral dministration), 24 hpi and 3 wpi. The total cardiac RNAs (n=3 animals per group) were extracted using a Qiagen RNA isolation kit. The ribosomal RNAs were depleted using a Qiagen rRNA HMR kit. RNA-seq libraries were constructed using a Qiagen Standard RNA Library kit followed by the manufacture manual. The insertion size of the library was determined using an Agilent 2100 Bioanalyzer system (Santa Clara, CA, USA). Library quantification was carried out using a NGS library quantification kit from Takara Bio.
library_name: WT_SPP1_3