title: GSM7496282: BCM-2277-107573-lungmet; Homo sapiens; Mus musculus; RNA-Seq
library_name: GSM7496282
library_construction_protocol: Qiagen RNeasy Library construction protocol: The RNA sample received was quantified using Qubit 2.0 Fluorometer (ThermoFisher Scientific,  Waltham, MA, USA) and RNA integrity was checked using TapeStation (Agilent Technologies, Palo Alto, CA, USA). The RNA sequencing  library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England  Biolabs, Ipswich, MA, USA). Briefly, mRNAs were initially enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 15  minutes at 94 °C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and  adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by  PCR with limited cycles. The sequencing library was validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA,  USA), and quantified by using Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) as well as by quantitative PCR  (KAPA Biosystems, Wilmington, MA, USA).