title: GSM7901299: hypothalamus_control_day14_rep1; Mus musculus; ssRNA-seq
library_name: GSM7901299
library_construction_protocol: Total RNA was extracted from frozen mouse hypothalamic (n=6) of 4 experimental groups (n=6: control d14, ExpOF d14, control d14+3, ExpOF d14+3) using RNeasy mini kit (Qiagen) according to the manufacturers' protocol. Messenger RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA protocol (Illumina). Poly-A containing mRNAs were purified by poly-T attached magnetic beads, fragmented, and cDNA was synthesized using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific). To prime for adapter ligation cDNA was adenylated and after a clean-up using AMPure beads (Beckman coulter), the DNA fragments were amplified using PCR followed by a final clean-up. Libraries were quality-controlled using a Bioanalyzer instrument (Agilent Technologies) and subjected to 51-bp paired-end sequencing on a NovaSeq 6000 (Illumina). A total of 2.74 billion reads were generated.