title: Haplotype for gene COI1 obtained from Sepiola atlantica
design: Amplicons were purified using QIAquick PCR purification kit (Qiagen, Valencia, CA) and quantified using a Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer (ThermoFisher Scientific, Waltham, MA). Purified amplicons were pre-sequenced using BigDye Terminator v3.1 (Applied Biosystems, Foster City, CA) and amplified on an MJ Research Dyad Disciple thermocycler (Waltham, MA). Pre-sequencing samples were cleaned using 96-well Sephadex plates (Edge Biosystems, St. Louis, MO). Samples were sequenced at the NMSU Molecular Biology Sequencing facility using the Applied Biosystems 3130XL sequencer (Applied Biosystems, Foster City, CA ) and at the UC Berkeley DNA Sequencing Facility (Berkeley, CA).
library_name: SEPATLHap2