title: GSM6783945: GD368 macrophages from pups with E.coli infection and with intact IL27RA, r1; Mus musculus; RNA-Seq
library_name: GSM6783945
library_construction_protocol: Spleens were homogenized in TRI Reagent® (Molecular Research Center, Cincinnati, OH). Using the commercial product protocol, the upper aqueous layer following phase separation was mixed with an equal volume of 75% ethanol and transferred to EZNA RNA isolation columns (Omega Biotek, Norcross, GA). The manufacturer's instructions were followed to complete the tissue RNA isolation. RNA isolation from approximately 7.5x104 splenic myeloid cells was conducted with the RNeasy Plus Mini Kit (Qiagen, cat#74134) according to the manufacturer's instructions. RNA-Seq libraries were built using KAPA mRNA HyperPrep Kit (KAPA Biosystems,Wilmington MA) with 100-500 ng of total RNA according to manufacturer's recommendation.