title: GSM7669044: non-hematopoietic Bone stromal cells at Postnatal day 30; Mus musculus; RNA-Seq
library_name: GSM7669044
library_construction_protocol: The surrounding muscle tissue was removed, and the epiphysis was detached from both the femur and tibia. The bones were then treated with collagenase and crushed to obtain a single-cell suspension. Afterward, the cells were washed, filtered, and resuspended in a blocking solution.  Lineage-negative cells were further enriched using CD45 and CD117 microbeads. The enriched cells were then processed for scRNA-seq using the BD Rhapsody Express system and the BD Rhapsody WTA Reganet kit. Sequencing was performed on an Illumina NextSeq 500 using a High Output kit (150 cycles).