title: GSM8239082: PGC, dRNA-ONT; Homo sapiens; RNA-Seq
library_name: GSM8239082
library_construction_protocol: Total RNA is extracted by TRIzol Reagent (Thermo Fisher Scientific), followed by polyA+ RNA enrichment using the Dynabeads mRNA Purification Kit (Thermo Fisher Scientific). For ESC, PE and PGC, ONT sequencing libraries are prepared using the SQK-PCS109 Kit for PCR-cDNA sequencing (cDNA-ONT), and the SQK-DCS109 Kit for direct RNA sequencing (dRNA-ONT). ONT sequencing is conducted using MiniON/GridION flow cells with R9.4.1 chemistry, with real-time base calling performed by the Guppy software. For ESC, DE, PE and PGC, the library preparation and sequencing of Illumina RNA-seq data are conducted by Novogene company.