title: GSM7670277: Plasma exosome HCC_2; Homo sapiens; miRNA-Seq
library_name: GSM7670277
library_construction_protocol: Exosome samples from plasma were centrifuged at 150,000 × g for 2 hours for twice.RNA was extracted by TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA was quantified using a Nano-Drop spectrophotometer (Thermo Fisher Scientific) miRNA sequencing libraries were prepared using the total RNA of each sample, denatured into single-stranded DNA molecules, captured on Illumina Flow cell, amplified into clusters in situ, and sequenced for 51 cycles on the Illuminia Nextseq 500 according to the supplier's instructions.