title: GSM8096009: untreated glioma, replicate 1; Mus musculus; RNA-Seq
library_name: GSM8096009
library_construction_protocol: Immediately after sacrifice, GBM tumor samples of mice treated with vehicle, RT, RT plus TMZ, RT plus TMZ and MET were collected and dissociated enzymatically to obtain a single-cell suspension with the Tumor Dissociation Kit – mouse (130-096-730, Miltenyi Biotech, Bergisch Gladbach, Germany) and GentleMACS Octo Dissociator (Miltenyi Biotec), according to the manufacturer's protocol. Directly after tissue dissociation, cell concentration and viability were assessed. Single cells were processed for transcriptome analyses using the Chromium Controller (10x Genomics) and the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry) (10x Genomics) with a cell recovery target of 4000 single cells. Libraries were prepared following manufacturer's directions. After quality controls, libraries were sequenced on Novaseq platform (Illumina) aiming at 50'000 reads/cell.