title: GSM6456324: 2afl/fl_OPC_03; Mus musculus; RNA-Seq
library_name: GSM6456324
library_construction_protocol: Single cell suspension from mouse brain tissue was prepared using Papain based Neural Tissue Dissociation Kit from Miltenyi Biotech. OPC and O4 cells were isolated using CD140a+ and O4+ microbeads respectively from Miltenyi Biotech. Total RNA was extracted using Qiagen microprep column kit and 900ng of total RNA per sample was used for RNA library preparation. RNA libraries were prepared using standard Illumina protocols for sequencing.