title: K562-RepliHiC-Release-3hr
design: Libraries were performed by Repli-HiC. Briefly, asynchronized cells were treated with BrdU for 15 min, released into fresh medium supplied with 100 uM thymidine for 3 hour, fixed, digested by HaeIII, dA-tailing, and ligated with biotinylated linkers. Fragmentated DNA were enriched by anti-BrdU and then biotin. After that, DNA were tagged with illunina adapters and sequenced. Biological replicate one.
library_name: K562-RepliHiC-Release-3hr