title: GSM8395112: Adipose tissue, Research participant 9 baseline; Homo sapiens; RNA-Seq
library_name: GSM8395112
library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit (#74106, Qiagen) according to the manufacturer's instructions.  RNA integrity was assessed using an Agilent bioanalyzer. Total RNA was sequenced by Novogene UC Davis.  Library construction, strategy, and data processing were performed by Novogene.  The details provided below are copied from the project methods document provided by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.  For the directional library, it was ready after end repair, A tailing, adapter ligation, size selection, USER enzyme digestion , amplification, and purification.