title: HsapiensBRCA24
design: For sample preparation, we used the NEB Next Ultra II End Repair and dA-tailing 440Kit (New England BioLabs Inc.). The native barcoding of amplicons was conducted in a 44120 L reaction volume, which included 1.5 L of DNA-repair amplicons, 2.5 L of Native 442Barcode EXP-NBD104 and EXP-NBD114 (Oxford Nanopore Technologies, Oxford, Ox- 443fordshire, UK), 10 L NEB Next Ultra II Ligation Master Mix (New England Biolabs, Ips- 444wich, MA, USA), 0.5 L of NEB Next Ligation Enhancer (New England Biolabs, Ipswich, 445MA, USA), and 5.5 L of nuclease-free water. The mixture was incubated for 20 min at 44620C, followed by 10 min at 65C. Subsequently, the barcoded amplicons were pooled. 447For sequencing, we employed the Ligation Sequencing Kit 1D (SQK-LSK110) accord- 448ing to the manufacturers protocol for adapter ligation. The library was then purified us- 449ing AMPure XP beads and quantified using a Qubit dsDNA HS Assay Kit. We loaded 50 450ng of the library onto an R.9.4.1 flow cell for sequencing on the GridION platform (Oxford 451Nanopore Technologies, Oxford, Oxfordshire, UK) over a period of 72 h. ONT MinKNOW 452software (Oxford Nanopore Technologies, Oxford, Oxfordshire, UK) was used to collect 453raw sequencing data. We confirmed the availability of active pores upon receipt and im- 454mediately before the sequencing run.
library_name: 24