title: GSM6696835: Custom library_48h; Homo sapiens; RNA-Seq
library_name: GSM6696835
library_construction_protocol: Nuclei isolation for snRNA-seq & ATAC-seq was optimized and performed following the demonstrated protocol CG000365 from 10x Genomics. The capture of mRNA and transposed DNA fragments was performed following 10xGenomics user guide CG000338. The snRNA-seq & ATAC-seq libraries were prepared following the 10xGenomics user guide CG000338. The custom libraries for TF barcode analysis was prepared using pre-amplified cDNA from Step 4 in protocol CG000338 by a two-step PCR process (See Methods).