title: Soil Microbiome 16S amplicon data
design: DNA extraction with 0.25 g of composite soil using QIAGEN DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA) following manufacture protocol. ITS1F and ITS2 primers used to amplify the internal transcribed spacer region (ITS) for fungal community according to Smith and Peays Illumina Miseq protocol (Smith and Peay, 2014). 515F and 806R primers used to amplify 16S V4 region for bacterial community following (Caporaso et al., 2011). PCR run in triplicates per sample and then pooled and cleaned using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Hoerdt, France). Cleaned PCR products were pooled to produce equimolar library mixture.
library_name: TU294