title: GSM7208894: 5xFAD, expr 3, scRNAseq; Mus musculus; RNA-Seq
library_name: GSM7208894
library_construction_protocol: Brain tissue was harvested and dissociated in less than an hour. Briefly, mice (12 ~ 24-week-old) were quickly decapitated after being CO2 anesthetized. The brain was cut into 1mm slices, and the hippocampus then was extracted. The samples were dissociated enzymatically to obtain a single-cell suspension with 0.05% papain and DNase 1 (DN25, Sigma-Aldrich, USA) in neurobasal media (Gibco, USA) at 37 °C for 30 min. After the enzymatic reaction was stopped by the addition of OIA, the cell suspension was passed through a 40-μm filter and washed by differential centrifugation to remove debris. The dissociated cells were put in Hank's balanced salt solution containing 0.04% bovine serum albumin and diluted to 100 cells/μl. Directly after cell counting, a cell suspension volume equivalent to 5000 target cells was used for further processing. Preparation of gel beads in emulsion and libraries were performed with the custom-made microfluidic device and inDropTM chemistry, according to the inDrop™ Library Preparation, Version 2.1 provided by the manufacturer. Libraries' quality and quantity were verified with a High-Sensitivity DNA Kit (Agilent Technologies, USA) on a 2100 Bioanalyzer (Agilent Technologies, USA). Next, sequencing was run in the rapid run flow cell and paired-end sequenced on a NovaSeq6000 (Illumina, USA).