title: GSM7209446: AT_30_RC17_hGPC_IFN_gamma_S39; Homo sapiens; RNA-Seq
library_name: GSM7209446
library_construction_protocol: RC17-derived glial progenitor cells were plated on laminin 521 (2μg/cm2) coated plates in a terminal differentiation medium. Cells were matured for 14 days and stimulated with IFN-g (5ng/ml) for 48hrs in the culture medium. Post stimulation, cells were washed twice with 1XPBS and detached with accutase and pellets were frozen down on dry ice for bulk-RNA seq. Total RNA was isolated using the Rneasy Mini Kit (Qiagen). Libraries were generated using Illumina TruSeq Stranded mRNA library prep kit (poly-A selection) and were sequenced on an Illumina NextSeq500 machine (paired-end 2×150bp).