title: GSM7670044: Oligodendrocyte, 30 mM Alcohol, biol rep 2; Mus musculus; RNA-Seq
library_name: GSM7670044
library_construction_protocol: RNA was isolated using the Qiagen RNeasy mini kit according to manufacturer's instruction. Less than or equal to 150 ng of total RNA was used for construction of the sequence libraries. 3' TagSeq was performed by the University of Texas Genomic Sequencing and Analysis Facility, based on the protocols from Lohman BK et al. (2016) and Meyer E et al. (2011). Libraries were quantified using the Quant-it PicoGreen dsDNA assay (ThermoFisher) and pooled equally for subsequent size selection at 350-550bp on a 2% gel using the Blue Pippin (Sage Science). The final pools were checked for size and quality with the Bioanalyzer High Sensitivity DNA Kit (Agilent) and their concentrations were measured using the KAPA SYBR Fast qPCR kit (Roche). Samples were then sequenced on the NovaSeq 6000 (Illumina) instrument with single-end, 100-bp reads.