title: GSM7669259: K562, etoposide or imatinib treated, RACE and RT-PCR targeted sequencing for novel transcripts, nanopore sequencing; Homo sapiens; OTHER
library_name: GSM7669259
library_construction_protocol: RNA was isolated with TRNzol Universal (Tiangen) and E.Z.N.A. Total RNA Kit I (Omega). The polyA+ fraction was isolated from each total RNA by Library Preparation VAHTSTM mRNA Capture Beads (Vazyme). Equal amount of polyA+ RNA from each sample was mixed and used for cDNA synthesis for RACE and RT-PCR. RACE and RT-PCR were performed to targeted enrich for InSETs, then sequencing library was prepared by Ligation Sequencing Kit (SQK-LSK109) (Oxford Nanopore Technologies Inc., Oxford, UK) by Baocheng Corporation (Hangzhou).