title: Illumina 300PE sequencing for Entamoeba marina SRT209 RNA
design: The total RNA was isolated from log-phase trophozoites of E. marina SRT209 grown in 15 mL polystyrene non-treated culture tubes using the Trizol reagent. A total of 8.18 ng of RNA was used for preparing RNA-seq library using ScriptSeq v2 RNA-seq Library Preparation Kit (Epicentre, WI, USA) following the manufacturers protocol. Sequencing was performed at the Pathogen Genomics Center (National Institute of Infectious Diseases, Tokyo, Japan) on the illumina MiSeq platform (300PE; illumina), yielding a total of 45.8 million reads (13.7 Gbp).
library_name: EmRNA