title: GSM6567854: Y551A_Dex_3 GH4288; Mus musculus; RNA-Seq
library_name: GSM6567854
library_construction_protocol: Each treatment condition was performed in triplicate. 5% Drosophila S2 cells were added to each harvested GR cell line to serve as spike-in control. RNA was extracted using the PureLink RNA Mini Kit (ThermoFisher Scientific) following the manufacturer's protocol. The quantity and quality of the RNA were assessed using the Agilent Bioanalyzer. Total RNAseq libraries were prepared using the Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit in the first batch, and TruSeq Stranded Total RNA Gold Kit with Ribo-Zero Plus was used in the second batch following the manufacturer's protocol. Barcoded libraries were pooled together and subjected to paired-end sequencing on a NovaSeq 6000 S1 Sequencer to generate 100 bp paired-end reads