title: GSM7207917: ATACseq-L-E145-Col2a1GFP-GFPp-R1; Mus musculus; ATAC-seq
library_name: GSM7207917
library_construction_protocol: After sorting, cells were centrifuged for 5′ at 400 × g at 4 °C and supernatant was discarded. Two biological replicates of 7.5 × 104 cells for each condition were then isolated for direct processing with Nextera Tn5 enzyme (Illumina, FC-131-1096). Samples were treated as previously described (67) with the exception that elution of the Tn5 treated DNA was performed using the Monarch PCR and DNA clean-up kit (NEB T1030S) following a 5:1 ratio of Binding Buffer:Sample. We then build libraries using customized NextSeq primers and Kapa HiFi HotStart ReadyMix (Roche, 07958927001). Libraries were sequenced on an Illumina HiSeq 4000 with paired-end reads of 50 bases.