title: GSM8393849: CBE-mRosa26_cDNA_mouse 5; Mus musculus; OTHER
library_name: GSM8393849
library_construction_protocol: Striatum extraction; RNeasy RNA extraction; qScript cDNA synthesis; amplicon sequencing with Nextera XT library consruction; MiSeq; RNA Star alignment, splice junction quantification, sashimi plot analysis on IGV Samples for DNA editing were extracted using Qiagen's DNeasy DNA extraction kit Samples for total RNA editing were extracted using Qiagen's RNeasy plus kit For Illumina Miseq experiments, libraries were constructed with the nextera XT indexing primer kits as per manufacturer's instructions Targeted genomic and exon high throughput sequencing with nextera indexing primers