title: GSM7903649: H526_dTAG_POU2AF2_DMSO_HAtag; Homo sapiens; ChIP-Seq
library_name: GSM7903649
library_construction_protocol: Chromatin immunoprecipitation experiments were carried out using the ideal ChIP-seq kit for transcription Factors (Diagenode) as per the manufacturer's protocol. Chromatin from 2×106 cells was used for each ChIP reaction with 4μg of the target protein antibody. In brief, cells were trypsinized and washed twice with 1× PBS, followed by cross-linking for 10 min in 1% formaldehyde solution. Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp. Fragmented chromatin was then used for immunoprecipitation using various antibodies, with overnight incubation at 4 °C. ChIP DNA was de-crosslinked and purified using the standard protocol.