title: GSM8232580: BrdU-ip-seq from HeLa-S3 cells grown in 1500 nM BrdU [IP]; Homo sapiens; OTHER
library_name: GSM8232580
library_construction_protocol: DNA was extracted using classical phenol:chloroform and precipitation techniques. Immunoprecipitated DNA was purified using AMPure XP beads at 0.9x. IP and input DNA were amplified separately using Illumina compatible indexes and NEBNext Ultra II Q5 Master Mix (M0544) for an equal number of cycles, typically 15-17, depending on recovery, and purified using AMPure XP beads at 0.9x.