title: GSM8186231: diabetic renal MEC 3; Mus musculus; OTHER
library_name: GSM8186231
library_construction_protocol: Mouse FFPE tissues were processed in accordance with the GeoMx® DSP Slide Preparation user manual (MAN-10150-01). Slides were baked for 3 hours at 60°C prior to deparaffinization. Proteinase K was used to digest the proteins. Slides were hybridized overnight with UV cleavable oligonucleotide probes then stained with morphology markers. Oligos from CD31+ and/or NeuN+ regions of interest were UV-cleaved and collected into a PCR plate for library prep. PCR products were purified and sequenced using a NovaSeq 6000 instrument. FASTQ sequences of barcode oligonucleotides were processed into digital count conversion (DCC) files using NanoString's GeoMX NGS Pipeline. Sequences that uniquely identify each probe are found in the PKC.