title: GSM8232217: CD20 nanoCAR T cell, 70 WT, rep1; Homo sapiens; RNA-Seq
library_name: GSM8232217
library_construction_protocol: mRNA was isolated using RNeasy Mini kit (Qiagen) per manufacturer's instructions. Library preparation was performed with the Illumina Stranded mRNA prep Ligation kit (20040534, Illumina) according to the manufacturer's instructions except for the following steps: i) six minute fragmentation, ii) 50 minute first strand cDNA synthesis and iii) additional Ampure bead clean-up at the end. The libraries were subsequently sequenced on a NovaSeq6000 (Illumina) paired-end (2x150bp) with an average of 40M reads per sample.