title: GSM6568319: ALF004C; Homo sapiens; RNA-Seq
library_name: GSM6568319
library_construction_protocol: RNA was extracted from whole lung tissue according to manufacturer's protocol (Fibrous Tissue Mini kit, Qiagen #74704). RNA quality was verified with the Agilent 2100 Bioanalyzer platform (Agilent Technologies). Samples with RNA integrity numbers (RIN) over 6 were used for RNA sequencing. cDNA libraries were prepared for sequencing using standard strand specific TrueSeq kits with ribosomal RNA and Globin mRNA depletion. At least 20 million 100 bp single-end reads were generated per sample using Illumina HiSeq 3000 (Illumina, California, USA) using standard protocols at the Australian Genome Research Facility (AGRF, Melbourne, Australia).