title: GSM8141512: SAN_2; Mus musculus; OTHER
library_name: GSM8141512
library_construction_protocol: Freshly isolated neonatal hearts were fixed in a methanol-free 4% paraformaldehyde solution (Thermo ScientificTM PierceTM, #28906) in PBS overnight at 4°C. Hearts were washed twice in PBS for 30 mins each, then were incubated in filtered 30% sucrose in PBS overnight at 4°C. Fixed cryopreserved hearts were embedded in Tissue-Tek® O.C.T compound (Sakura, #4683) and immediately frozen on powdered dry ice. Embedded hearts were sectioned using a cryostat at 8 um thickness. Following ROI selection, DNA oligos were cleaved from RNAScope probes by directing UV light at each ROI using the digital micromirror device. Cleaved oligos were then collected into the wells of a 96-well collection plate, which were subjected to library preparation and next-generation sequencing using Manufacturer's protocol