title: RNA-Seq of Zanthoxylum armatum DC. Fruit
design: After the RNA samples were tested, eukaryotic mRNA was enriched with magnetic beads with Oligo(dT) (in case of prokaryotes, mRNA was enriched by removing rRNA through the kit) . Subsequently, mRNA was broken into short fragments by adding fragmentation buffer. One-stranded cDNA was synthesized using six-base random primers (random hexamers) with mRNA as template, and then two-stranded cDNA was synthesized by adding buffer, dNTPs, and DNA polymerase I and RNase H. Subsequently, two-stranded cDNA was synthesized by utilizing the Double-stranded cDNA was purified using AMPure XP beads. purified double-stranded cDNA was first subjected to end repair, addition of A-tail and ligation of sequencing junctions, followed by fragment size selection using AMPure XP beads. Finally, PCR amplification was performed and the PCR products were purified by AMPure XP beads to obtain the final library. After the library was constructed, Qubit 2.0 was used for preliminary quantification to dilute the library, and then Agilent 2100 was used to detect the insert fragment size of the library, and after the insert fragments met the expectation, the effective concentration of the library was accurately quantified using the Q-PCR method to ensure the quality of the library.
library_name: FRAS210001337-1a