title: Pooled Amphibian Skin and Environmental Swabs from Bedford, NY
design: UsingilluminaMiSeq250bpsequencing,weamplifiedtheV4regionofthe16SrRNAgeneusingthe515Fand806Rprimers.DNAfromamphibianskinsamplesandsurroundingenvironmentwasusedattheDNAtemplate. Sample Barcode information is given below: Sample = BarcodeSequence; ANNDRY = CCTGAACT; ANNH2O = CTTCGGCA; ANNMUK = ACGGGACA; BOGDRY = GTCATATC; BOGH2O = GGAAACCA; BOGMUK = TTGCGCAT; TUNDRY = ACATTCAG; TUNH2O = TCACGGGA; TUNMUK = ATGAGACT; VPDRY = CTTGTGTC; VPGF10 = TCGAGGAC; VPGF11 = CAGGCGTA; VPGF12 = GGATCGCA; VPGF15 = AGCTGTTG; VPGF16 = GGATGGTG; VPGF18 = TCCGAATT; VPGF2 = TTAGGGCT; VPGF3 = GCTGATGA; VPGF5 = TAGGATTG; VPGF7 = CACGCCAT; VPGF7b = TCTAGCGT; VPGF9 = GCGATATA; VPH2O = TGAGCCGG; VPMUK = GCGGCAAT; VPPF1  = CCAGTGTA; VPPF10 = TCCAAAGT; VPPF11 = TACAGATG; VPPF12 = ACACCTGG; VPPF13 = ACACGTAA; VPPF15 = CGGAGCTA; VPPF16 = TATCGTTG; VPPF16b = TTACTGTG; CPPF17 GAGTGGTA;  VPPF18 = GACTTGGT; VPPF19 = TACACGAT; VPPF2 = GGAGACAA; VPPF20 = GAAGTTGG; VPPF21 = TTCCTAGG; VPPF22 = GCACACAC; VPPF23 = GTCTAATT; VPPF24 = CGATCCGT; VPPF25 = AGGCTACA; VPPF3 = AATCAGTC; VPPF4 = AAGAGATG; VPPF5 = AAGGAGCG; VPPF6 = CCTCGTTC; VPPF7 = AATCCGTA; VPPF9 = ACGTGTAC; VPTAD1 = GGCCACGT; VPTAD12 = CTGCTAAC; VPTAD18 = ACGCGCAG; VPTAD20 = ATCCCGAA; VPTAD21 = CAGTGCAT; VPTAD22 = GCACGACA; VPTAD25 = GCACGACA; VPTAD34 = TAGGAACT; VPTAD35 = ATCTCCAC; VPTAD38 = ACGCCACG; VPTAD4 = TAGCTCGT; VPTAD40 = GTTGGTCA; VPUPDRY = GAACTAGT; VPUPH2O = GACGGAAC; VPUPMUK = CAAGCATG; VPWF1 = GAATCTTC; VPWF10 = GTATGCG; VPWF11 = GTACATAC; VPWF12 = GACAGGAG; VPWF18 = GTCAATTG; VPWF19 = TCCTCTGT; VPWF20 = GGTGTCTA; VPWF21 = TCCGACAC; VPWF4 = AATGCCTC; VPWF5 = GAACCAAA; VPWF6 = CTAGCGAA; VPWF7 = AGTAGAGG; VPWF8 = CGCAGCGG; VPWF9 = ATCGATCT
library_name: PooledMRGRanidSamples2017