title: Illumina NovaSeq 6000 paired end sequencing of SAMD00615530
library_name: let-7 High
library_construction_protocol: The cells were loaded onto a Chromium Next GEM Chip G Single Cell Kit (10x Genomics, USA). The Gel Beads-in-emulsion (GEMs) generation and barcoding, reverse transcription, and cDNA generation and library construction were performed following the manufacturer?s protocol (Chromium Next GEM Single Cell 3? Reagent Kits v3.1 Dual Index, 10x Genomics). Dual-indexed, single-cell libraries were pooled and sequenced in paired-end reads on Novaseq (Illumina).