title: AT1R WT RNA seq 1
design: RNA obtained from sorted BM AML cells of two AT1R+/+ and two AT1R/ AML cells transplanted mice, was purified with QIAGEN miRNeasy Mini Kit. RNA was fragmented, double-stranded cDNA was generated from fragmented RNA, and the index-containing adapters were ligated to the ends. Libraries were constructed following the manufacturers protocol with reagents supplied in Illuminas TruSeq mRNA stranded sample preparation kit. Samples were pooled and sequenced on Illumina Novaseq S4 300 cycle flowcell according to the manufacturer's recommendations to achieve 20 million paired reads per sample. The differentially expression genes were identified using standard DESeq2 workflow.
library_name: c