title: GSM8239156: Patient #13; Homo sapiens; RNA-Seq
library_name: GSM8239156
library_construction_protocol: RNA was extracted using RNeasy Micro Kit [Qiagen, Cat #74004] Extracted RNA was quantified with a Qubit fluorometer (Cat #Q33238, Thermo Fisher Scientific), and RNA quality control was performed with a Bioanalyzer RNA 6000 Pico Chip (Cat #5067-1513, Agilent Technologies, CA). Samples with an RNA Integrity Number (RIN) >7 passed quality control and were used for library preparation with the Illumina Stranded mRNA Library Prep Kit according to manufacturer's instructions (Cat #20040532, Illumina Inc., San Diego, CA). This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR amplification and validation.