title: GSM8281754: Control3, snRNAseq; Homo sapiens; RNA-Seq
library_name: GSM8281754
library_construction_protocol: Nuclei were isolated from flash-frozen ovarian tissues from five CG and four control patients using a modified method from 10X genomics chromium nuclei isolation kit (dx.doi.org/10.17504/protocols.io.6qpvr4jb2gmk/v1).  The nuclei were purified with washing in nuclei suspension buffer and sucrose density gradient centrifugation. Freshly prepared 8,000 nuclei were loaded onto Chromium microfluidic chip used to generate single-cell gel bead emulsions using the 10x Genomics Chromium controller. Later cDNA amplification was performed, and libraries were prepared as per the 10x Genomics manufacturer's protocol. Libraries were pooled and sequenced on S2 flow cell (paired-end 100 bp) using an Illumina NovaSeq 6000 platform. snRNAseq libraries from nuclei isolated from ovarian tissue were constructed using 10x Genomics Chromium Next GEM Single Cell 3ʹ Kit v3.1