title: GSM8007307: B cells, KO1; Mus musculus; RNA-Seq
library_name: GSM8007307
library_construction_protocol: Total RNA was used as input material for the RNA sample preparations. Briefly,  mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads.  Fragmentation was carried out using divalent cations under elevated temperature in  First Strand Synthesis Reaction Buffer(5X). First strand cDNA was synthesized using  random hexamer primer and M-MuLV Reverse Transcriptase, then use RNaseH to  degrade the RNA. Second strand cDNA synthesis was subsequently performed using  DNA Polymerase I and dNTP. Remaining overhangs were converted into blunt ends  via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments,  Adaptor with hairpin loop structure were ligated to prepare for hybridization. In order  to select cDNA fragments of preferentially 370~420 bp in length, the library fragments  were purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR  amplification, the PCR product was purified by AMPure XP beads, and the library was  finally obtained.