title: GSM8281672: sgZbtb20_T1(2w)_ATO_Thy_FB; Mus musculus; RNA-Seq
library_name: GSM8281672
library_construction_protocol: For single-cell RNA sequencing using, M-ATO thymocytes were isolated by FACS sorting at different timepoints (Week 2: day 12, Week 3 to 4: day 27), isolating with Live+CD45.2+CD3+TCRb+. Single cell suspensions were run on a Chromium X instrument and libraries prepared following the Chromium Next GEM Single Cell 3' RNA-Seq Assay v3.1 Dual Index with Feature Barcode technology for CRISPR Screening user guide (10x Genomics, CG000316, RevD)128. We targeted 6,000 cells and used 12 cycles of cDNA amplification. Sample quality was confirmed using a Qubit (RNA HS kit; Thermo Fisher) to determine concentration and a Fragment Analyzer (Agilent) to determine RNA integrity. Every sample had an associated feature barcode except the control libraries.