title: GSM8141508: SUM159 cell SMARCA4 shRNA knockdown spheroids; Homo sapiens; ATAC-seq
library_name: GSM8141508
library_construction_protocol: Spheroids were dissociated with Accumax (Sigma) to generate single cell suspension. Dead cells were removed with the dead cell removal kit (Miltenyi, 130-090-101) to achieve at least 85% of live cells assessed by flow cytometry. ATAC-seq library was prepared with Nextera DNA library preparation kit (Illumina)