title: GSM7670121: Mouse; Replicate 3_LPS; Mus musculus; RNA-Seq
library_name: GSM7670121
library_construction_protocol: Total RNA was extracted from 2 × 106 PMφs using RNeasy mini kit (Qiagen, #74104). To eliminate genomic DNA, 20 U of Roche RNase-free DNase I (Roche, Cat#4716728001) was added in the eluant (100 µl), followed by 20 min of incubation at 37°C.  Total RNA was quantified by NanoDrop Spectrophotometer ND-1000 and 750 ng was used to enrich mRNA using NEBNext poly(A) mRNA magnetic isolation module (New England Biolabs Cat#E7490). Library construction used NEBNext Ultra II directional RNA library prep kit (New England Biolabs Cat#E7760) and NEBNext multiple oligos for Illumina (New England Biolabs # E7335, E7500, E7710, E7730). Libraries were PCR-amplified for 10 cycles, quantified by MiSeq Nano V2, and sequenced on an Illumina NovaSeq S1 flow cell with 150 bp paired-end run to obtain about 30 million reads per sample