title: GSM6734160: Foxc1b_120hpf; Danio rerio; RNA-Seq
library_name: GSM6734160
library_construction_protocol: Zebrrafish embryos from the transgenic line Tg[foxc1b:GFP] were collected every 24 hours between 48hpf and 144hpf. The embryos were anesthetized with 3-amino benzoic acid ethyl ester (Tricaine). Then, their eyes were dissected and subsequently collected on ice, before being incubated for 8 minutes in 0.25% Trypsin with EDTA at 37°C. After filtering, the dissociated single cells were sorted for GFP+ at the University of Kentucky Flow Cytometry and Immune Monitoring Core at the Markey Cancer Center. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).