title: GSM6940976: He_400_2; Heterosigma akashiwo; RNA-Seq
library_name: GSM6940976
library_construction_protocol: Total RNA was extracted by TRIzol reagent (Invitrogen, USA) following the manufacturer's instructions. For each sample, approximately 5 µg of RNA was used to construct a cDNA library. The RNA quality was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). First-strand cDNA was then generated to create the final cDNA library on an Illumina platform (San Diego, USA) with 90 nucleotide pair reads. The cDNA library was sequenced using paired-end reads of 90 nucleotides on an Illumina HiSeq 2000 (San Diego, USA).