title: GSM6568129: WT-W3.5 replicate 1; Hordeum vulgare; RNA-Seq
library_name: GSM6568129
library_construction_protocol: Total RNA was extracted from 15–20 spikes for each of three biological replicates using TRIzol (Invitrogen) and purified using a RNeasy Micro Kit (Qiagen) following the manufacturer's instructions. Library preparation was performed using 1μg of high-integrity total RNA (RNA integrity number > 8) using the TruSeq RNA Library Preparation Kit v.2 (Illumina, RS-122-2101 and RS-122-2001), following the manufacturer's instructions.