title: GSM8232174: FB, scramble, replicate2; Homo sapiens; RNA-Seq
library_name: GSM8232174
library_construction_protocol: Total RNAs from cultured cell line or cultured cells with different treatments were extracted with Trizol Reagent (Invitrogen) according to the manufacturer's protocol. To profile RNA subcellular distribution in FB neuron cells, total/cytoplasmic/nuclear RNAs were processed with the RiboMinus Transcriptome Isolation Kit (Human/Mouse Module, Invitrogen) to deplete ribosomal RNAs for sequencing (ribo– RNA-seq) The ribo– RNA-seq libraries from  circRTN4(2,3) KD FB neuron cells were prepared using KAPA RNA HyperPrep Kit, followed by size selection using KAPA Pure Beads.