title: GSM7051592: DPASEKO_Steadystate_rep1; Mus musculus; RNA-Seq
library_name: GSM7051592
library_construction_protocol: Trizol (Life Technologies) was used to extract total RNA from sorted pre-selection DP thymocytes. Template-switch RACE was used to ligate adapaters to the 5' ends and amplify Tcra transcripts. Illumia adapters were added to the 5' template-switch oligo and the antisense Trac-specific primer.