title: GSM7875206: CD4_TRAPPC1_FRESH_2; Mus musculus; RNA-Seq
library_name: GSM7875206
library_construction_protocol: Naive CD4+ T cells and naive CD8+ T cells were sorted by CD4+/CD8+CD62L+CD44low from the mLNs and spleens of 4 weeks old WT and CD4-cre Trappc1 cKO mice. The total RNA of the sorted naive CD4+ T cells and naive CD8+ T cells were extracted using TRIzol reagent (Thermo Fisher, 15596018), and sequencing were done by the Annoroad Gene Technology Co., Ltd (Beijing). Differential expressed genes were carried out by DEseq2 software38. KEGG pathway enrichment was performed by KOBAS 3.0.28 (http://kobas.cbi.pku.edu.cn/)37,59. Protein and protein interaction network were analyzed by String (https://string-db.org/) and visualized by Cytoscape60. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.