title: GSM7770410: PBMCs, Renal irAEs, sample3; Homo sapiens; RNA-Seq
library_name: GSM7770410
library_construction_protocol: Patients' blood  was collected on the first day of each hospitalization. Blood samples from the participates were collected into the EDTA anticoagulant tube for later management. Then PBMCs were separated on the day of blood collection, and Ficoll-Paque density gradient centrifugation was used to isolate PBMCs. Cell viability was assessed by microscopic examination using Taipan blue staining. Finally, samples with more than 85% viability can be used to conduct single-cell RNA-seq libraries. Single-cell RNA-seq libraries were generated using Chromium Single-Cell 3' Library and Gel Bead Kit v2 (10×Genomics, Pleasanton, CA) according to the manufacturer's instructions. Then, the libraries were read on  Illumina NovaSeq 6000 system.