title: RNA-Seq of RPE WT Mus musculus
design: We performed Bulk RNA sequencing (RNASeq) of choroidal tissue from adult WT and Lgals1-/- mice as previously described by Lehmann, GL et al. (J Exp Med. 2020 Jun 1; 217(6): e20190730). Three mice per group were euthanized and their eyes were enucleated and any remaining extraocular tissue attached to the sclera was removed. The neural retina was carefully detached from the optic nerve and RPE/choroid and discarded RPE/choroid tissue was mechanically dissected from the sclera using a scalpel and immediately used for RNA extraction (RNeasy Mini Kit, Qiagen). Sample quality control, library preparation and sequencing were performed at the Weill Cornell Medicine Epigenomics Core. Briefly, cDNA libraries were prepared with the TruSeq RNA Sample Preparation Kit (Illumina) and sequenced on an Illumina HiSeq2000 platform.
library_name: wt