title: RNA-seq of strawberry fruit shelflife
design: Fragaria ananassa cv. Elsanta strawberry fruit at red ripe stage were washed with water, the calyx was removed using a sharp blade, and they were immersed in 200 ppm sodium hypochlorite for 2 min. They were then air dried in a laminar air flow hood and halved. Halved fruit were stored at 8C and sampled for ChIP and RNA extraction at 0 and 5 days of storage. RNA was extracted according to Greco et al. (2014) https://doi.org/10.1371/journal.pone.0096470. RNA quality and concentration were verified by agarose gel electrophoresis, a Nanodrop spectrophotometer (Thermo Scientific) and an Agilent 2100 Bioanalyzer (Agilent). RNA was used for library preparation (poly A enrichment), tested using a Qubit 2.0 and an Agilent 2100 to test for concentration and insert size.  Sequencing used an Illumina NovaSeq 6000, generating  40M paired-end reads per sample. Library preparation and sequencing was performed by Novogene Co., Ltd. (Beijing, China).