title: NGS of healthy donor (HBV negative)
design: Viral probes for capture were designed according to different HBV genomes (A, B, C, D, E, F, G, and H) and produced by iGeneTech (iGeneTech, Beijing, China). 200 ng of Genomic DNA from HBV-infected HepG2-NTCP cells were sheared into fragments at 150-200 bp, and the library was constructed by IGT Enzyme Plus Library Prep Kit V3 (iGeneTech, Beijing, China). The cfDNA of HBV-infected clinical samples was directly used for library construction (IGT Fast Library Prep Kit v2.0, iGeneTech, Beijing, China) according to the manufacturer's instructions. The 200 ng HBV probe was added to the 750ng sample library to target the entire HBV genome for sequence capture and enrichment (TargetSeq One Hyb & Wash Kit v2.0, iGeneTech, Beijing, China).