title: Nanopore sequencing of Escallonia herrerae
design: High molecular weight DNA was isolated from nuclei pelleted from leaf tissues, sheared to 25 kb with a g-tube (Covaris,Woburn, MA) according to the manufacturer's instructions. DNA was size selected using the Circulomics Short Read Eliminator -XS kit (PacBio, Menlo Park, CA) and sent to the Interdisciplinary Center for Biotechnology Research at the University of Florida for Nanopore library construction and PromethIon sequencing.