title: genomic TSS-seq of S. cerevisiae: WT, with MPA treatment
design: Total RNA was extracted by a phenol-chloroform method (Schmitt, Brown, & Trumpower, 1990), followed by RNA purification (RNeasy Mini kit, QIAGEN, 74104) with on-column DNase digestion (RNase-Free DNase Set, QIAGEN, 79254) and rRNA depletion. TSS-seq, including enzymatic treatments, adapter ligation, and library selection, etc) was done using procedures described in (Vvedenskaya, Goldman, & Nickels, 2015).