title: RNA-Seq of Danio rerio: 8mpf male and female liver
design: The procedures for the library construction included mRNA purification by poly-T oligo-attached magnetic beads, RNA fragmentation, reverse transcription using random hexamer primers and M-MuLV reverse transcriptase, followed by RNA degradation with RNase H treatment, second strand cDNA synthesis with DNA Polymerase I, blunt ends repair, 3 ends adenylation, adaptor ligation, 370~433bp cDNA fragments selection using the AMPure XP system (Beckman Coulter,Beverly, USA). Library was amplified with Phusion High-Fidelity DNA polymerase and quality assessment.