title: RNAseq of Mus musculus right frontal lobe tumor grown from Homo sapiens melanoma cell line
design: Samples were FACS sorted to obtain Lin+ and Lin- fractions. RNA was extracted from samples with Qiagen's RNAeasy Plus kit, ribosomally depleted using Takara's Ribogone Mammalian kit. cDNA was made using Clontech's SMARTer Universal Low Input RNA Kit for Sequencing, and barcodes were added using the Ion Plus Fragment Library Kit. Two individually barcoded samples were sequenced together on an Ion Torrent S5XL by the Analytical and Translational Genomics Shared Resource at UNMCCC.