title: RNA-seq of mus musculus: brain tumor immune microenvironment
design: CD45+ immune cell were selected from GBM tumor microenvironment following tissue dissociation and positive cell selection. Celero EZ DNA-Seq, 24 reactions kit (Tecan Genomics) was utilized to generate sequencing libraries following the manufacturers instructions. RNA-sequencing was performed using Illumina NovaSeq 6000, SP 2 x 50.