title: RNA-Seq of Homo sapiens: Gallbladder Cancer
design: Total RNA was isolated from approximately 25-30mg tissue using RNA Easy Mini kit according to the manufacturers instructions. Quality of total RNA was checked using Agilent RNA 6000 Nano chip in 2100 Bioanalyzer (Agilent) and quantitation was performed by NanoDrop spectrophotometer followed by flurometric method in Qubit fluorometer. Total RNA samples with high RNA integrity number (RIN) were selected for sequencing library preparation using Kapa mRNA Hyperprep Kit according to the manufacturers protocol. Paired end 2 x 100 bp sequencing of these libraries were performed in Novaseq 6000 (Illumina).