title: Transcriptome sequencing and denovo assembly of Sardinella longiceps
design: Total RNA was extracted from 5 tissues (muscle, gill, liver and gonad) using Trizol method. After pooling the sample in Equimolar concentrations, RNA library preparation was performed with NEBNext Poly(A)mRNA magnetic isolation module kit and NEBNext Ultra RNA library preparation kit and sequenced using illumina HiSeq 2500 paired-end 150 base pair cycle.