title: SHfxSDm, 12th generation, heat-selected line 2
design: First-generation (F1) hybrids were generated between SD and SH copepods, and 100 gravid females used to start the replicate line in 400ml beaker. After 100 gravid females of the next generation were observed, they were transfered to new beaker and immersed at the target temperature (34-36C) for 1 hour.Three days after treatment, 50 individuals amongst survivors were randomly picked and their offspring allowed to start the next generation. This process was repeated until the 12th generation, with target temperature adjust as needed to impose ~25-50% mortality. At the 12th generation, ~100 adult male copepods were picked and pooled for genomic DNA isolation. A whole-genome DNA library was prepared with a Nextera kit using 25-40ng of DNA as input.