title: RNA-Seq of Healthy BM CD34+ 3 Untreated
design: mRNA was extracted from primary samples using a miRNeasy micro kit (Qiagen). The Ribo-Zero Gold kit (Illumina) was used to remove cytoplasmic and mitochondrial ribosomal RNA. RNA-Seq libraries were generated using a KAPA stranded RNA-Seq library preparation kit (KAPA Biosystems) on a Sciclone platform (Perkin Elmer). mRNA underwent fragmentation, cDNA synthesis, and next-generation library synthesis via exome capture and PCR amplification. Libraries were sequenced on a Next-Seq 500 instrument (Illumina).