title: 16S sequencing of mus musculus vaginal microbiota: nondiabetic and diabetic pregnancy
design: Bacterial DNA was extracted from thawed PBS swab samples with the Quick-DNA Fungal/Bacterial Microprep kit following the manufacturers protocol (Zymo Research) with final elution in 20 L of water. The v4 region of the bacterial 16S rRNA gene was amplified by PCR using primer 515F and 806R and sequenced on an Illumina MiSeq v2 using the 2250bp paired-end protocol by the BCM Alkek Center for Metagenomic and Microbiome Research (CMMR).