title: MiSeq
design: Total DNA extraction was performed using a DNA kit (BioBasic). Bacterial 16S ribosomal DNA amplicons were prepared by amplification of the 16S rRNA gene V3-V4 variable region with PCR primers U341F and 805R using the HotStarTaq Plus Master Mix Kit (Qiagen, USA). PCR products were purified using calibrated Ampure XP beads and used to prepare illumina DNA library.