title: 16s rRNA gene-Seq of beka squid fish sauce
design: The sequencing pipeline was conducted according to methods outlined in Kozich's study (2017). The V4 region of the 16S rRNA gene of eluted DNA from samples were amplified via PCR using dual-index primers. Successful amplification was confirmed with gel electrophoresis on a Geldoc c235 (Azure Biosystems, California, USA), and the DNA concentration of each amplified products was equalized using the SequalPrep Normalization Plate Kit (Applied Biosystems, Massachusetts, USA). All equalized DNA samples were pooled in a LoBind microcentrifuge tube (Eppendorf, Hamburg, Germany) to create a pooled library.