title: RNA-Seq mus musculus: adult male cortex
design: Total RNA was extracted using TRIzol Reagent with genomic DNA removing by Dnase I and only high-quality RNA sample (OD260/280 = 1.82.2, OD260/2302.0, RIN6.5, 28S: 18S1.0, >1 g) was used for constructing sequencing library. The TruseqTM RNA Sample Prep Kit (Illumina, FC-122-1002) was used for library construction under the manufacturers instructions. Then the paired-end RNA-seq sequencing library was sequenced with Illumina HiSeq xten/NovaSeq 6000 sequencer (2  150 bp read length). The raw paired end reads were trimmed and quality controlled by SeqPrep and Sickle with default parameters. Then clean reads were separately aligned to reference genome (GRCm39) with orientation mode using HISAT2 software. The mapped reads of each sample were assembled by StringTie in a reference-based approach.