title: WGS_T10_wastewater metagenome_AS effluent
design: Samples were collected in duplicate for DNA extraction. Samples (2 mL for WW and biomass in AS lines and PSBR, 200 mL for the effluents) were centrifuged for 25 min at 13,000 rpm, pellet were recovered, and the total DNA was extracted using the DNeasy PowerSoil kit (QIAGEN, Germany) according to manufacturers instructions. The recommended initial step of vortexing was carried out in an Eppendorf ThermoMixer Comfort (Germany) at 1,400 rpm for 10 minutes. The extracted DNA was quantified using Qubit (Thermo Fisher Scientific, USA) and quality was checked through gel electrophoresis on 1% (w/v) 1TAE agarose gels. Prior to shotgun analysis, DNA was stored at -80C. Shotgun analysis was performed at FISABIO (Valencia, Spain). Samples were sequenced through the Illumina NexSeq500 platform with 150 bp paired-end chemistry.