title: 16s rRNA
design: DNA was extracted using the DNeasy PowerLyzer PowerSoil Kit, and DNA was amplified using universal primers of F515 (GTGYCAGCMGCCGCGGTAA) and R926 (CCGYCAATTYMTTTRAGTTT) to target the V4 and V5 regions of bacterial 16S rRNA fused to Illumina adaptors and barcode sequences. Individual samples were barcoded and pooled to construct the sequencing library, followed by sequencing with an Illumina MiSeq instrument to generate pair-ended 2300 reads