title: 16S rRNA gene amplicon of silage
design: Dry barley in the 3rd period. The DNA extraction was performed as described by Yu and Morrison (2004). For bacterial amplicon sequencing, universal primers targeting 16S rRNA gene V4 region were used (Caporaso et al., 2011). Libraries were prepared and sequenced on Illumina MiSeq platform using 2 x 250 bp chemistry.