title: scRNA-Seq of Gallus gallus: embryonic 10, male, Daheng broiler leg muscle
design: Single cell barcoding and library preparation were performed based on 10Genomics single-cell RNA sequencing platform (10Genomics, Pleasanton, CA, USA). The filtrated single cells prepared above were assessed using a hemocytometer (Thermo Fisher Scientific) and the cell concentration was adjusted to 1,000 cells/l. Reverse transcription and 10barcoded cDNA library was performed by the 10Genomics Chromium Single Cell 3' Library & Gel Bead Kit v2 (10Genomics, Pleasanton, CA, USA) and 10Genomics Chromium barcoding system following the instructions provided by the manufacturer. The sequencing of prepared libraries was performed using Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA) as per the manufacturers instructions.