title: C3-Genome
design: For PacBio HiFi sequencing, young leaf tissue of a single individual of I. puberulus sampled in Igap population C3 was sent to Arizona Genomics Institute (The University of Arizona, USA). Total genomic DNA was extracted from this sample using the cetyl trimethylammonium bromide method (CTAB) (Doyle & Doyle 1987). A whole-genome shotgun sequencing library was prepared with SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, CA), including DNA fragmentation, DNA damage repair and end repair, hairpin adapter ligation, and DNA purification. Long-read High fidelity - HiFi sequencing was performed on a Sequel IIe in circular consensus sequencing (CSS) mode