title: non-acclimated to lipid sludge
design: Bioinformatic processing was done using the QIIME Pipeline Version 1.9.1. Low quality reads were filtered (criteria: coefficient greater than 25) and sequences were trimmed to remove primers, barcodes, and adapters. Effective reads were obtained which were further processed to remove chimera and noise. Operational taxonomic units (OTU) were made using the UClust algorithm (Edgar, 2010) with a 97% identity threshold. The Silva database, release 132, was used for classification with a confidence threshold of 80%.