title: Genomic Sequence of Alcohol Non-Preferring Rat
design: Spleen samples from 15 alcohol nave rats of the P (7 male and 8 female) and NP (8 male and 7 female) rat lines were collected and stored at -80C. DNA was isolated using Gentra Puregene Kits (Qiagen, Redwood City, CA, USA). About 25 mg of spleen was homogenized in cell lysis solution, followed by proteinase K digestion, RNase-A incubation, and DNA precipitation as described in the manufacturers protocols. DNA purity (260/280 ratio) measured 1.69-1.91, with the majority >1.80. The DNA yield was more than 400 g in most samples