title: RNAseq of Trypanosoma brucei Mut sample
design: PolyA RNA extracted from T. brucei cultured exclusively expressing V5-tagged PIP5Pase mutant D360A/N362A. cDNA obtained using ProtoScript II Reverse Transcriptase, fragmented to sizes between 0.3 and 1 kb. Library prepared for oxford nanopore sequencing.