title: RNA Iso-Seq of Crepidula atrasolea embryos and adult
design: 300ng of total RNA were converted to cDNA with the Iso-Seq Express Oligo Kit. cDNAs were barcoded with the Barcoded Overhang Adaptor Kit 8A, and converted into a library with the SMRTBell Express Template Prep kit 2.0 (Pacific Biosciences). The libraries were pooled in equimolar concentration and the pool was sequenced on 1 SMRTcell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 30hs movie time.