title: mtDNA:Crossaster japonicus
design: Tissue was harvested from the tube feet of experimental animals and mitochondria were isolated using the Mitochondria Isolation Kit (QIAGEN); mitochondria DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN); libraries were constructed with the QIAseq FX single cell DNA library kit and analyzed by NGS with Illumna, Hiseq 400 ro HiSeqx system.