title: WGS of Macrostomum lignano DV1_10 subline: adult F1 offspring from pool A, replicate 1
design: We established two new sublines, DV1_8 and DV1_10, with the karyotypes 2n=8 and 2n=10, respectively, differing with the copy number of the largest chromosome MLI1. Each subline was initiated from two karyotyped individuals of the inbred line DV1 and cultivated for six months (~8 generations) under standard laboratory conditions. We generated five pools of worms from each subline, DV1_8A-E and DV1_10A-E; each pool was initiated from 20 juvenile worms. Their offspring F1 was regularly collected and cultivated separately until their maturation. F1 generation of each pool was characterized by karyotyping of 40 specimens. Extraction of gDNA from each pool of F1 worms (N = 60 for each sample) was performed using the standard phenol-chloroform method. Purified gDNA was fragmented with dsDNA Fragmentase (New England Biolabs, USA). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) according to the manufacturer's protocol and sequenced on the Illumina NovaSeq 6000 platform (NovaSeq S Prime flow cell).