title: Amplicon sequencing; metagenome : museum,textile
design: Amplicons were generated in triplicate for each sample using Kapa HiFi DNA polymerase (Roche, Basel, Switzerland) and purified by AmPure XP magnetic beads (Beckman Coulter). DNAs concentration was assessed with QbiteTM Fluorometer and dsDNA HS Assay Kit (ThermoFisher Scientific). Amplicons libraries were quality checked using KAPA qPCR Library Quantification Kit (KAPA-Roche, Basel, Switzerland), pooled in equimolar ratio and indexed according to Nextera indexing strategy by PCR (Illumina). Amplicons were sequenced on MiSeqTM System instrument (Illumina, San Diego, CA) in the DNA Sequencing and Oligonuleotide Synthesis Laboratory (IBB PAS) in paired-end mode using MiSeq Reagent Cartridge 600 cycle v3 chemistry kit (Illumina, San Diego, USA).