title: RNA-seq of mus musculus: brain tumor immune microenvironment
design: CD45+ immune cell were selected from GBM tumor microenvironment following tissue dissociation and positive cell selection. SMARTer Mouse TCR a/b Profiling Kit (Takara Bio) was utilized to amplify TCR beta chains using PCR and sequencing libraries were generated following the manufacturers instructions. RNA-sequencing was performed using Illumina MiSeq x 300.