title: mtDNA-seq of Chordata : surface water samples
design: Residual components from PCR reactions were removed by using the AMPure XP beads kit (Beckman Coulter, Life science, In, USA) according to the manufacturer recommendations. Then, the index PCR program followed: 94C, 3 min; 8 cycles at 94C, 30 sec/55C, 30 sec/68C, 30 sec; and 68C, 5min. As previously, residual components from index PCR were removed by using the AMPure XP beads kit. All samples were quantified by using QuantiFluor DsDNA System Kit (Promega, Madison, USA) and pooled in 70 L of reaction tube to obtain a final concentration of 1.5 ng/L before being sent to the Centre dexpertise et de services Gnome Qubec (Montral, Canada).