title: Fungal and bacterial profile of insect frass, i.e. cattle dung digested by Oryctes rhinoceros.
design: Fungal ITS and bacterial 16S profiles of frass collected in the Animal Rearing and Containment Unit, Icipe, Kenia, in 2022. The frass was produced from Oryctes rhinoceros feeding on cattle manure for 28 d. The experiment was performed in three independent biological replicates. For community profiling, the total DNA was extracted from the frass samples collected from the independent biological replicates. From the same DNA extract both the fungal ITS2 region and V4 region were sequenced by Illumina Miseq sequencing. Files with 16S and ITS extension contain bacterial and fungal sequences, respectively. Independent samples = separate files (R1, R2) were collected from the different independent biological replicates.