title: Mitochondrion-Seq of Gonatopsis borealis musculsu: carcass
design: 1. With 1  G DNA initial quantity database;2. Covaris M220 ultrasound breaks DNA to 300~500bp;3. Leveling, adding A at 3 'end, and connecting index connector (TruSeq  Nano DNA Sample Prep Kit4. The library was enriched and 8 cycles were amplified by PCR;5. 2% agar gel to recover the target band (Certified Low Range Ultra Agarose);6. TBS380 (Picogreen) is quantitative, and the computer is mixed according to the data proportion;7. Carry out bridge PCR amplification on cBot solid phase carrier to generate clusters;8. Illumina NovaSeq sequencing platform for 2 * 150bp sequencing