title: Pyrosequencing sequencing of ITS1 region of fungal hyphae: isolated from mesh bags buried in a spruce and fir forest
design: Every sample from each plot was amplified individually with a primer unique to that plot, and all amplicons (nine successful  samples from each plot) were pooled in an equimolar mixture by combining equal amounts of each PCR amplicon at a standard concentration. The final 20 ng  L1 mixture, containing six unique pyrosequencing primers, each representing one of six different plots, was amplified in a 1/8 plate Next Generation pyrosequencing reaction on a Roche GS-FLX at the McGill University and Genome Quebec Innovation Center.