title: genomic DNA extraction of MMR- K12 E. coli after 15 days of mutation accumulation in liquid culture
design: overnight culture of E. coli in 10mL glass tube with loose metal fastener/breathable lid was resuspended. Resuspension was DNA-extracted with the Wizard dna extraction protocol from promega, following manufacturer's instructions. 1 microgram Genomic DNA was sent to Beijing Genomics Institute for genomic DNB-seq.