title: Barcode seq of WT and mutant Pseudomonas inoculant culture also containing Rahnella OV744
design: Microbial genomic DNA obtained from plant dissects and inoculum mixtures was amplified to enrich the barcode locus using primers suitable for sequencing adapter attachment depending on the intended sequencer (Table 4). Amplicons were then pooled at equimolar concentration and sequenced using an in-house Illumina MiSeq sequencer, as well as commercially via Illumina NovaSeq technology (VANTAGE, Vanderbilt University, Nashville, TN). From the resulting data, barcodes were extracted from sequencing reads by Bartender v1.1 [41], summarized, and then analyzed in R (v 4.0.3) using the tidyverse package (v 1.3.0) and a custom visualization script ().