title: Pool 4 of bacterial plasmids containing VHH genes
design: Amplicons used had approx 400 pb, marked with CS1/CS2 tails. Barcoding compatible with Illumina. DNA was quantyfied with Bioanalyzer, pooling, washing using diverse methods and titration by qPCR. Library construction and whole-genome sequencing were performed by the Unidad de Genomica (Parque Cientifico de Madrid). Pair-end libraries (2 x 300) were sequenced using a MiSeq Illumina system. Approx 500,000 reads were obtained per amplicon. Filtering by quality and split by barcodes to generate the final FASTQ files.