title: Furfural/HMF utilizing engineered strain of Pseudomonas putida DSM 6125
design: Samples DNA was extracted using the MagMAX Microbiome Ultra Nucleic Acid Isolation Kit. Samples were prepared for whole genome sequencing using an Illumina DNA Prep tagmentation kit and unique dual indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling. Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v3.10.12, an on-board analysis software on the NextSeq2000.