title: IsoSeq of ice plant
design: The cDNA was size selected and put into two groups: those  2.5 kb and those > 2.5 kb in length. Fragments larger than 2.5 kb were subjected to reamplification, and subsequently, cDNA from both size categories were pooled equimolarly in preparation for sequencing. A single SMRT (Single-molecule real-time sequencing) cell run was performed with a movie time of 30 hours.