title: PacBio long read sequencing reads for Spondylurus nitidus
design: Tissues were homogenized on ice using a sterile razor blade. ATL buffer (140 l) and Proteinase K (60 l) were then added to the homogenized material and incubated at 65C for 1 hr. The 200 l of resulting lysate was used as starting material for the PCI extraction following a PacBio recommended protocol. An additional round of SPRI bead cleaning was done to eliminate impurities to meet the DNA requirement for PacBio sequencing. In particular, to achieve 260/280 nucleic acid purity ratios of 1.82.0. DNA concentration was determined with the Qubit dsDNA HS Assay Kit, and high molecular weight content was confirmed by running a Femto Pulse. HMW DNA was sent to Maryland Genomics for HiFi library preparation and sequencing with PacBio Sequel II SMRT Cells.