title: RNA-Seq of Nipponacmea schrenckii: foot muscle tissue
design: For Illumina pair-end sequencing of each tissue, at least 3g genomic DNA was used for sequencing library construction. Paired-end libraries with insert sizes of ~400bp were prepared following Illuminas standard genomic DNA library preparation procedure.Purified genomic DNA is sheared into smaller fragments with a desired size by Covaris, and blunt ends are generated by using T4 DNA polymerase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test. At last, the qualified Illumina pair-end library would be used for Illumina NovaSeq 6000 sequencing.