title: 1H-ST-1
design: RNA samples were fragmented by heating at 95C for 10 min and annealed to biotinylated random primers. A 5 adaptor, which contained an Illumina primer, was added to the sequence. First-strand cDNA was synthesized by reverse transcription (RT). Then, Illumina primers were used to obtain double-stranded cDNA by the PCR method. cDNA fragments of 300 to 500 bp were harvested by gel extraction and then directly amplified with a TruSeq PE cluster kit (Illumina, America). Sequencing reactions were performed on an Illumina HiSeq 2000 sequencer.