title: ddRAD-seq of Mediodactylus kotschyi
design: The double?digest restriction site?associated DNA (ddRAD) libraries were prepared following the protocol of Peterson et al. (2012). Briefly, about 750 ng of high?quality DNA was simultaneously doubled?digested using SbfI and MspI restriction enzymes. The individual barcoding was followed by the selection of fragments using the Blue Pippin electrophoresis platform under the range selection of 415-515 bp. Targeted fragments were amplified through 11 cycles of Polymerase Chain Reaction. Libraries were pooled and sequenced.