title: ABE8e+site3_OT4-6&ABE8e+site23_OT1-5
design: HEK293T cells were transfected with base editor ABE8e guided by sgRNAs 4~6 for the off~targets of site3 respectively, and with ABE8e guided by sgRNAs 1~5 for the off~targets of site23 respectively sorted based on mCherry fluorescence, and lysed for barcoded targeted amplification sequencing.