title: RAD-seq of Eliurus ellermani : adult ear biopsy
design: Samples were digested with SbfI enzyme before P1 adapter ligation. After adapter ligation, samples were sheared in a Covaris M220 to obtain fragments with an average size of 500 bp. Following end repair, we conducted size selection using AMPure XP beads. After 3adenylation, we ligated the P2 adapter. The libraries were finally enriched with 10 cycles of PCR. Libraries quality was verified using Fragment Analyzer and quantified using qPCR.