title: 16S analysis of Mice colonized with a synthetic microbiota composed of 13 bacteria species (SM13) were fed  inulin diet for 30 days - subject SM13-I-928
design: 16S rRNA gene libraries were constructed using primers to amplify the V4 region. Amplicons were generated using a high-fidelity polymerase (AccuPrime; Invitrogen) and then were purified using a magnetic bead capture kit (Ampure; Agencourt) and quantified using a fluorometric kit (QuantIT PicoGreen; Invitrogen). The purified amplicons were then pooled in equimolar concentrations using a SequalPrep plate normalization kit (Invitrogen), and the final concentration of the library was determined using a SYBR green quantitative PCR (qPCR) assay with primers specific to the Illumina adapters (Kappa). Libraries were mixed with Illumina-generated PhiX control libraries and our own genomic libraries and denatured using fresh NaOH.