title: Hi-C data from Spondylurus nitidus (Spon15)
design: Prior to Hi-C preparation, tissues from the same sample were homogenized using a sterile razor blade on ice. A library was prepared from muscle tissue from one Spondylurus nitidus individual (Spon15). An in situ Hi-C library was prepared as described in Rao et al. (2014) with some modifications. Briefly, after Streptavidin Pull-down, the biotinylated Hi-C products underwent end repair, ligation, and enrichment using the NEBNext Ultra II DNA Library Preparation Kits. Then, titration of the number of PCR cycles was performed as described in Belton et al. (2012).