title: PEACE of E.coli : CTG
design: the above-sorted recombinant E. coli BL21 (DE3)/MP/TP-T3-GFP and E. coli BL21 (DE3)/TP-T7-GFP cells were incubated at 37C and 200 rpm for 4~6 h, respectively, then, their respective plasmid mixtures were extracted using SanPrep column DNA gel extraction kit (Sangon Biotech Co., Ltd. Shanghai, China), and 426 bp of the egfp of the mixture was amplified with GNGS-F and GNGS-R as primers. The PCR products were sent to Sangon Biotech Co., Ltd. for NGS, and the mutation diversity and rate of egfp was analyzed according to the report provided by the company.