title: MDA-MB-231 EP300 ChIP-Seq
design: ChIP-Seq: Cells were cross-linked with 1% formaldehyde (Merk) in PBS for 10 min at room temperature. H3K4me3 was immunoprecipitated with anti-H3K4me3 antibody (#9751), EP300 with anti-EP300 (#54062) antibody from Cell Signaling Technology and Dynabeads Protein G (Thermofisher Scientific) as previously described (Biomed Pharmacother. 2023 Dec;168:115798. doi: 10.1016/j.biopha.2023.115798). Libraries were constructed with MGIEasy Cell-free DNA Library Prep Set and sequenced on DNBSEQ-G400 using DNBSEQ-G400RS High-throughput Sequencing Kit (FCL SE100) from MGI.