title: Metatranscriptomics of Homo sapiens: infant gut
design: cDNA was amplified using Ultra directional RNA kit, following protocol for Ribosome Depleted RNA. Qubit/ PicoGreen was applied to the barcoded cDNA libraries, and the final library was processed by BioAnalyze on DNA chip to determine size. Diluted to 4 nM, denatured and loaded on NextSeq high output 150bp x 2 sequencing kit at 1.9 pM with 1% PhiX spike-in as standard.