title: RNA-Seq of RAW264.7 cells :treat with poly(I:C) and Nobiletin
design: Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure.mRNA was purified using Dynabeads Oligo (dT) (Thermo) and fragmented using Magnesium RNA Fragmentation Module (NEB, USA). SuperScript II Reverse Transcriptase (Thermo) was used for reverse transcription. Sequencing was performed on a Novaseq 6000 (Illumina, USA).