title: DNA-Seq of environmental sample: wastewater primary influent
design: Whole genome library construction QC, pooling, and NextSeq sequencing of user prepared DNA. A minimum of 100ng of DNA (based on Qubit) will be used for library preparation using the Illumina DNA prep to an average size of ~350bp. Libraries will be pooled in equimolar amounts for final QC and sequencing. Library QC includes qPCR, Qubit, and Bioanalyzer. Sequencing will be 75 x 75bp with dual (10bp) index read. Phi-x will be spiked in to monitor run performance and maintain sequencing diversity. Sequencing output is expected at 350 Million clusters (reads) per lane for an average of ~50M reads per sample.