title: ATAC-seq library generated from fresh A549 cells
design: ATAC-seq experimental libraries were generated using A549 human lung carcinoma epithelial cells (ATCC, VA, catalog #CCL-185). Three biological replicate libraries (2501_001, 2501_002, 2501_003) were prepared from freshly harvested cells using an ATAC-seq kit (Active Motif, 53150) following the manufacturer's protocol. The remaining five libraries (2501_007, 2501_008, 2501_018, 2501_019, 2501_020) were prepared using cryopreserved cells following methods outlined in Milani et al. 2016 with modifications (see Roth et al. 2023).