title: Oxford nanopore long read sequences fastq of HVKP1
design: genomic DNA was extracted from overnight cultures grown at 37C in Mueller Hinton broth, using Fire Monkey High Molecular Weight DNA Extraction Kit (RevoluGen). Libraries were prepared using a ligation sequencing kit (SQK-LSK109) and native barcoding kit (EXP-NBD104) according to the manufacturers guidelines (Oxford Nanopore Technologies). Long-read sequencing was performed using an R9.4.1 flow cell (Oxford Nanopore Technologies) on a MinION device, running for 72 h. The reads were base called using a high accuracy base calling mode, and demultiplexed using Guppy (v6.2.1). Seqkit (v2.2.0) was used to determine number of basepairs, number of reads and the read N50