title: Reduced representation (ddRAD) sequencing of Helioseris cucullata
design: Library preparation was carried out using a modified version of the ddRAD protocol by Peterson et al. (2012). DNA was double digested with EcoRI-HF and Sphl enzymes. Adapters were ligated, and fragments were size selected using Pippin Blue. DNA fragments were PCR-amplified to add the Illumina adapter sequences.