title: RNAseq of Strongylocentrotus purpuratus 48h embryo
design: Total RNA was extracted using the Qiagen Rneasy Mini kit with Dneasy column following the manufacturers specifications. RNA concentration and quality were assessed using a nanodrop (Invitrogen) and integrity was visualised in an agarose gel. After quality assessment, TruSeq standard mRNA libraries were prepared and sequencing was performed with an Illumina NextSeq 500 platform using 75bp paired-end sequencing. The samples were mapped onto the newest reference genome (Spur v5.0) obtained from Echinobase (Arshinoff et al., 2022) using STAR (version 2.7.5c) (Dobin et al., 2013), with the standard parameters for paired reads.