title: RNAseq of Methylobacterium mesophilicum: replicate 1
design: Total RNA isolation was performed using Trizol reagent and Purelink RNA Mini Kit. Total RNA samples were purified using DNase provided by the Illustra RNASpin Mini RNA isolation kit. RNA was quantified using Quant-iT RiboGreen RNA Assay kit, and rRNA depletion was performed using Ribo-Zero Magnetic kit (Bacteria). cDNA libraries were constructed using TruSeq RNA sample preparation kit v2. Libraries were quantified using absolute qPCR in a 7500 Real Time PCR System with the Kapa Library Quantification kit for Illumina.Sequencing of cDNA libraries were performed in a MiSeq equipment with a final pool concentration of 8 pM (first run) and 10 pM (second and third run). All runs used the MiSeq Reagent Kit v2 of 500 cycles with Paired-end 2x250 sequencing strategy.