title: RNA-Seq of mus musculus: adult female colon
design: RNA extracted from female mouse colon of sham surgery mouse fed CBD; data was extracted using RNeasy Plus Universal Mini Kit (Catalog#73404, QIAGEN, Hilden, Germany ). The concentration, quality, and integrity of total RNA was determined using a Nano Drop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and RNA samples were provided to Azenta (South Plainfield, NJ, USA) for Standard RNA-Seq processing. Paired-end sequencing was performed using Illumina HiSeq 2  150 bp paired-end configuration yielding 124,739 Mbases and 415,802,210 reads.