title: AYBE+site3_OT1-3&AYBE+site11_OT1-5
design: HEK293T cells were transfected with base editor AYBEv3 guided by sgRNAs 1~3 for the off~targets of site3 respectively, and with AYBEv3 guided by sgRNAs 1~5 for the off~targets of site11 respectively sorted based on mCherry fluorescence, and lysed for barcoded targeted amplification sequencing.