title: mRNAseq Nematostella high density replicate 2 adult 4
design: Total RNA was extracted from whole animals at the end of the population density experiment period. Sample cDNA libraries were prepared from an average of 21-274ng of high-quality RNA. Library preparation and sequencing was done by Novogene Inc. Sample libraries were constructed following manufacturer protocols using an Illumina Stranded mRNA Prep kit, which uses poly A enrichment to purify mRNA from total RNA, and an Illumina NovaSeq 6000 was used for sequencing (paired end, 150bp reads).