title: BRfxSDm inbred line 155, control temperature (20C)
design: Adult copepods (25-30 individuals, approximately equal sex ratios) were transfered to 15ml tubes containing 5ml of filtered artificial seawater and tubes were immersed in a water bath at 20C for one hour. After treatment, tubes were immersed in 20C water for a 15 min recovery. Copepods were then rapidly transferred to a centrifuge tube and homogenized in Trizol and RNA isolation performed with Zymo Direct-zol kits. RNA-seq libraries were prepared with NEBNext Ultra II Directional RNA kits (with polyA selection), using 300-400 ng of total RNA as input.