title: BR-7day
design: Total DNA was extracted from 30-40 mg cecal contents using a QIAamp PowerFecal Pro DNA kit per manufacturers instructions (QIAGEN, Hilden, Germany). The cecal DNA concentration was measured using a Qubit dsDNA HS Assay kit (Invitrogen, Waltham, MA, USA). 16S rRNA full-length amplificons were generated with barcoded Full-Length 16S primers from Pacific Bioscience (PacBio) and the 2 KAPA HiFi HotStart Ready Mix (Forward sequence: AGRGTTYGATYMTGGCTCAG; Reverse sequence: RGYTACCTTGTTACGACTT). Amplicons were converted to a library with the SMRTBell Express Template Prep kit 3.0. The library was sequenced on SMRTcell 8M on a PacBio Sequel IIe sequencing platform using the CCS sequencing mode, at the Roy J. Carver Biotechnology Center, University of Illinois.