title: single cell RNA sequencing sequencing of human: imr-90 fibroblast
design: For RNA sequencing, cDNA was prepared using the Smart seq 4 kit (Takara) using manufacturer recommended protocol. Briefly, the RNA of the cytosolic fraction(11l) was used as input for conversion. The product was quantified via Qubit (ThermoFisher Scientific) and quality was assessed using a HS5000 screen tape on the TapeStation (Agilent). Following this, sequencing libraries were prepared utilizing the Nextera XT kit (Illumina) with a 4minute tagmentation step and 13 cycles of amplification followed by a purification using AMPure beads (Beckman Coulter). Libraries for whole genome sequencing (WGS) were prepared according to the previously described scMDA protocol using a NEBNext Ultra II FS kit (NEB) [4]. For quality control purposes, a locus drop out test (LDO) was performed using primers and SYBR reagent [11]. Library size was assessed with a TapeStation (Agilent) using HS5000 screen tape for quality control of cDNA and HS1000 screen tape for all final libraries.