title: RNASeq of combined triplicate Mycobacterium ulcerans moved back to control fromTemp 37C and aerobic for 24H
design: RNA libraries were created from combined triplicate replicates of M. ulcerans RNA samples under aerobic and 300C (T0-T48H), aerobic and 370C (T24 H), microaerophilic and 370C (T24 H),  and samples that were transferred from 370C, aerobic or microaerophilic conditions to aerobic and 300C at 48 H [aerobic and 370C (T48 H), microaerophilic and 370C (T48 H)] conditions. Libraries were created using the NEBNext Ultra RNA Library Prep Kit and NEBNext Multiplex Oligos (Dual Index Primers) for Illumina and associated protocols. High-throughput RNA sequencing was performed by St. Jude Childrens Research Hospital on an Illumina HiSeq2000 with 2 X 150bp PE (paired end) read lengths.