title: RNASeq of forager bee during apricot bloom
design: Extracted totRNA was treated with an rRNA depletion step using the RiboMinusTMPlant Kit for RNA-Seq (Invitrogen, Waltham, MA, USA) as per the manufacturers instructions.Ribo depleted totRNA and dsRNA HTS libraries were generated using theIllumina TruSeq Stranded mRNA Library Prep kit, following the manufacturers protocol,starting after mRNA selection steps [48]. An Illumina NextSeq500 was used to generatesingle-ended 75 base read files to each sample. Libraries were dual indexed using the IDTfor Illumina TruSeq RNA UD Indexes (Illumina, San Diego, CA, USA) and normalized.Then, 2432 sample libraries were pooled and sequenced using a NextSeq500 high outputkit v2.5, 75 cycles (Illumina) which generated between 10 and 16 million reads, on averagefor each sample.