title: DNA sequence of mustard greens planted soil from each individual plot A3
design: The V4 variable region of the 16S rRNA gene was amplified by using the 515F and 806R specific primer. PCR reactions were carried out with Phusion High-Fidelity PCR Master Mix. The PCR products were purified using Qiagen gel extraction kit. The libraries were generated with TruSeq DNA PCR-Free sample prepara-tion kit and analyzed by HiSeq2500 according to the manufacturers instructions.