title: HI-C for Gorsachius magnificus:adult male musle
design: For Hi-C sequencing, we fixed muscle tissue from the same male individual intended for de novo genome sequencing with 37% formaldehyde. Following a 10-minute incubation at room temperature, we halted the cross-linking reaction with 2.5% formaldehyde. We then collected the precipitated cells for Hi-C library preparation. A single Hi-C library was constructed and subjected to paired-end sequencing with 150 bp reads on the Illumina NovaSeq 6000 Sequencing System