title: Archaeal and Bacterial 16S rRNA gene amplicon of AM_5_generation_16S
design: The amplicon product was then sequenced by a NovaSeq6000 sequencer (Illumina) with paired-end 250 bp mode (PE250). All the sequence data were first filtered to remove the low-quality reads. Further reads were analyzed according to the Qiime2 pipeline. (universal primers targeting both bacteria and archaea with barcode 515FmodF/806RmodR)