title: Long Read DNA-seq of Brachyteles arachnoides: adult male blood
design: Genomic DNA of Brachyteles arachnoides was isolated from blood with Blood & Cell Culture DNA Maxi Kit (Qiagen). A single library of long reads was prepared from an aliquot of 20 g of the original DNA sample, diluted to 100 ng/L. This sample was sent to RTL Genomics diluted in DNAStable Plus (Biometra). Sequencing was performed in six SMRT Cells on a PacBio Sequel platform (instrument SN 54112) at 20-fold coverage depth. The SMRT cells were sequenced with 600min movie lengths.