title: Male#2
design: The genomic DNA was digested with EcoRI and the digested DNA samples were further fragmented using a Covaris ultrasonic crusher (Woburn, Massachusetts, USA). The samples were subsequentially subjected to end filling, A-tailing, sequencing index ligation, PCR amplification, purification and fragment selection to construct sequencing libraries. The libraries were quality-checked and subjected to 150 bp paired end (PE) sequencing using an Illumina high-throughput sequencing platform.