title: RNA-seq of pyrus P163-1
design: Total RNA from leaves was isolated using TRIzol reagent (Invitrogen) following the manufacturers instructions. The purity of the extracted RNA was detected by a spectrophotometer (NanoDrop 2000&8000), and the concentration and integrity of the RNA were detected by a bioanalyzer (Agilent 2100, Agilent Technologies, Palo Alto, CA, USA). Three biological replicates of RNA-seq libraries were constructed for each group. Sequencing was performed using an Illumina platform (NovaSeq)