title: GSM8242224: DNMT3L_OE_BTS11_3; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Aliquots of 5000 cells suspended in PBS were thawed and then lysed in 10mM Tris buffer with 0.5% SDS and proteinase K at 37°C for 1 hour. Bisulphite conversion was done using the EZ DNA Methylation Direct Kit (Zymo Research), as per the manufacturers protocol. First and then second strand synthesis was performed on eluted DNA using Klenow Fragment (3'->5' exo-) enzyme (New England Biolabs) and customised 9bp random sequence containing biotin-conjugated adaptors. Using Phusion High-Fidelity DNA polymerase (New England Biolabs), libraries were amplified for 10 cycles in a 50uL reaction.