title: GSM6737845: VIC, F33; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed using Buffer RLT and β-mercaptoethanol and total mRNA was isolated following manufacturer's instructions of the Qiagen RNeasy mini kit and treated with DNase (Qiagen). Quantity and quality of RNA samples were analyzed for the presence of 18S and 28S ribosomal RNA with appropriate A260/A280 and A260/A230 ratios using the NanoDrop 2000. First-strand cDNA synthesis (after ribosomal RNA was removed) and library preparation was performed using the Takara SMARTer Stranded Total RNA- HI Mammalian Library Prep Kit (Takara Bio).