title: GSM7724514: ATAC-seq_N_A; Homo sapiens; ATAC-seq
library_construction_protocol: The first step is to extract the nucleus. After extraction, the cell counter is used to count and 50,000 nuclei are taken for next step. The Tn5 transposase carrying part of the joint incubated with 50,000 nuclei , cut, extracted DNA The transposed DNA was purified with a MinElute Kit (Qiagen) and was used to generate the library via PCR amplification. All libraries were purified using a Qiagen MinElute PCR Purification Kit following the manufacturer's instruction and were sequenced on an Illumina Novaseq 6000 platform.