title: GSM7848346: 100-40-mJ-cm2-48-hr_R1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Fibrous Tissue Kits (Qiagen Inc., cat #: 74704) according to the manufacturer's instructions (with 700 µL of the mixed lysate transferred to a RNeasy Mini column and repeated until all lysate had been used). During the procedure, Qiagen's On-Column RNase-free DNase was used to eliminate possible DNA contamination. Library preparation for next-generation sequencing was conducted on randomized RNA samples using the TruSeq-stranded mRNA kit (Illumina, San Diego, California) according to the sample preparation guide. The input RNA for library preparation was 300 ng per sample. A dual index scheme was used to multiplex samples for sequencing, which were pooled and sequenced on high output flow cells using an Illumina NextSeq® 500. Single-end sequencing was performed with a read length of 75 bp. A sequencing depth ~40 million reads per sample was achieved.