title: GSM8189743: ChIP-seq CHD8 E14 forebrain CHD8 KI rep2; Mus musculus; ChIP-Seq
library_construction_protocol: For RNA-seq, total RNA was extracted from the forebrain of a male mouse at E14.5 or E18.5 with the use of the TRIzol reagent and PureLink RNA Mini Kit (Thermo Fisher Scientific). Library preparation and sequencing analysis on an Illumina NovaSeq 6000 were conducted by Novogene Co., Ltd (Beijing, China). For ChIP-seq analysis, purified DNA was used to prepare a library with the use of SMARTer ThruPLEX DNA-Seq kit and SMARTer DNA unique dual index kit. For ATAC-seq analysis, nuclei were used prepare a library with the use of an ATAC-Seq Kit (Active Motif). Sequencing analysis on an Illumina NovaSeq 6000 were conducted by Novogene Co., Ltd. RNA-seq, ChIP-seq, and ATAC-seq libraries were prepared for sequencing using standard protocols