title: GSM8241352: LM_2; Rattus norvegicus; RNA-Seq
library_construction_protocol: The fresh ventricles were immediately separated from the atria and flash frozen. Subsequently, the ventricles were homogenised with pestle and mortar in liquid nitrogen and RNA was extracted using miRNeasy Tissue/Cells Advanced Mini Kit (QIAGEN, The Netherlands), including the DNase digestion protocol. Libraries for massive parallel RNA sequencing (RNA-seq) were prepared with the QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen) and sequenced, in two runs, with the use of a HiSeq X Ten platform, which generated at least 20 million reads/sample (150 bp Paired End).