title: GSM7139804: PSCs, TNFa-/IFNg-treated, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Four wells of cells cultured in the same condition were pooled for RNA extraction. RNA extraction was performed using the RNeasy Mini kit (Qiagen, 74106). Cultured cells were directly lysed in RLT buffer (Qiagen). Sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep kit (20020594). Briefly, 4 μg total RNA per sample were used to prepare cDNA libraries. cDNA was amplified with 12 PCR cycles to minimize duplicate reads. Libraries were pooled and sequenced with single-end 76 bp reads on an Illumina NextSeq 500, using the High Output kit.