title: GSM6736813: IL-12/18 Adult [scATAC-seq]; Mus musculus; ATAC-seq
library_construction_protocol: FACS-sorted cells were permeabilized and nuclei were isolated using a lysis buffer containing 0.1% Igepal CA-630, 5mM Tris-Cl pH 8.0, 150mM sucrose, 5mM NaCl, 1mM MgAc2 and 3mM CaCl2 Cells were counted and 3-8k isolated nuclei per sample were loaded in the Chromium controller for the formation of gel bead-in-emulsions (GEMs) following the manufacturer's instructions (10X Genomics). The single-cell ATAC-seq libraries were prepared using Chromium Next GEM Single Cell ATAC Kit v2 following the accompanied protocol and were sequenced on Illumina NextSeq500.