title: GSM7505139: dbp2-aa-R-2; Saccharomyces cerevisiae; ssRNA-seq
library_construction_protocol: Total RNA was extracted from cells grown to mid-log phase (OD600 0.5) using standard hot phenol procedure. Extracted RNA was ethanol-precipitated, resuspended in nuclease-free H2O (Ambion) and quantified using a NanoDrop 2000c spectrophotometer and/or a Qubit fluorometer with the Qubit RNA HS Assay kit (Life Technologies) RNA-seq libraries were prepared from 125 ng of rRNA-depleted RNAs using the TruSeq Stranded Total RNA kit (Illumina)