title: ATAC-seq of Fairchild mandarin
design: sorted nuclei were incubated with 2l Tn5 transposomes in a 40 l tagmentation buffer (10mM TAPS-NaOH pH8.0, 5mM MgCl2) at 37C for 30min without rotation. The integration products were purified using a Qiagen MinElute PCR Purification Kit or NEB Monarch DNA Cleanup Kit and then amplified using Phusion DNA polymerase for 1013 cycles.