title: RNA-Seq of G. hirsutum: Coker 201 "20 days" RKN-treated biprep 1
design: Frozen root samples from Coker 201 and M-120 RNR collected at six different time points were individually crushed with mortar and pestle in liquid nitrogen, and total RNA was extracted using Spectrum Plant Total RNA extraction kit (Sigma-Aldrich) following manufacturers protocol. Georgia Genomics and Bioinformatics Core at the University of Georgia, Athens, GA performed library construction using Kapa Stranded RNA-Seq kit from Roche and sequencing using a NextSeq PE150 High OutPut flow cell platform.