title: GSM8145373: A3_200min-arrest_RNA-rRNAdep; Escherichia coli K-12; Caulobacter vibrioides; RNA-Seq
library_construction_protocol: Cells in PBS as mixed with 250 ul TRI Reagent (Zymo Research) and lysed by bead beating on a Fastprep 24 (MPbio). Cell debris was pelleted (14k rpm, 2 min) and the supernatant was recovered. RNA was then extracted using the direct-zol RNA microprep kit (Zymo Research). rRNA was depleted using the NEBNextrRNA Depletion Kit for Bacteria (NEB, #E7850) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760) was then used to prepare libraries for paired-end (2x150bp) Illumina sequencing (Novogene).