title: GSM7135724: E14-WT paused, time 0, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from equal number of ESCs (5x10^5 cells),RNA extraction using phenol-chloroform extraction, as per manufacturer's instructions. RNAs were treated with 10mM iodoacetamide and ethanol precipitated. Library preparation was done with Quant-seq 3′-end mRNA library preparation (Lexogen) using 100ng RNA.