title: GSM6596658: sh_NC, replicate 2, mRNA-derived cDNA & ADT-derived cDNA; Mus musculus; RNA-Seq
library_construction_protocol: Gene and protein expression libraries were prepared using Chromium Next GEM Single-Cell 3' Gel Bead and Library Kit (10x Genomics, V.3.1) according to the manufacturer's instructions GEM-RT was performed in a 96-Deep Well Reaction Module: 53 oC for 45 min, 85 oC for 5 min; end at 4 oC. After RT, GEMs were broken down, then the cDNA and DNA barcodes were cleaned up with DynaBeads MyOne Silane Beads. cDNA was amplified with 96-Deep Well Reaction Module: 98 oC for 15 s, 67 oC for 20 s, and 72 oC for 1 min; 72 oC for 1 min; end at 4 oC. Followed by size selection to separate the amplified cDNA molecules for 3' Gene Expression and Cell Surface Protein library construction SPRIselect Reagent.