title: GSM7785437: EXOSC2 AID ESCs, HiC, 0 hr, rep2; Mus musculus; Hi-C
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was subjected to MboI digestion, biotin end repairing and ligation, then reverse-crosslinking and DNA purification. The purified DNA was then sonicated and subjected to biotin pull down by C1 Streptavidin beads. Library construction procedures including end repairing, dATP tailing and adaptor ligation were proceeded on beads. Finally, the DNA was eluted and subjected to PCR and size selection for sequencing. HiC libraries were constructed following TruSeq DNA preparation procedures including end repairing, dATP tailing, adaptor ligation and PCR amplification.