title: RNA-Seq of Wistar rat: colon tissue
design: Total RNA was extracted and its concentration and quality were determined. Paired-end library was prepared according to the instructions of ABclonal mRNA-seq Lib Prep Kit. The mRNA was purified and then fragmented. Using mRNA fragments as templates, the first and second strands of cDNA were synthesized. The synthesized double-stranded cDNA fragment was connected to the linker sequence for PCR amplification. The PCR products were purified and the library quality was evaluated using Agilent Bioanalyzer 4150. Finally, the length of PE150 on DNBSEQ-T7 sequencing platform was used for sequencing.