title: Nanopore sequencing of BrdU-labled osteosarcoma cells: KSS mitochondrial genome cybrid
design: Cybrid cells formed by the fusion of enucleated but mitochondria-intact Kearne-Sayre Syndrome patient fibroblasts with mitochondria-depleted 143B cells were cultured in DMEM/10%FBS.  24 hours after plating, 200uM BrdU was added to the media, and cells were harvested 24h after BrdU addition.  DNA (including nuclear and mitochondrial) was collected via Quick-DNA kit (Zymo) and prepared for Nanopore sequencing using a Rapid Barcoding Kit (Oxford Nanopore).  No special steps were taken to enrich for mitochondrial DNA.  Library was sequenced for 24h on an R9.4.1 flow cell on a minION mk1C device.