title: GSM6433465: MAF HD replicate 1 (RNA); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using PureLink RNA mini kit (Invitrogen) following the manufacturers' instructions. RNA samples were quantified using Qubit Fluorometric Quantification (Thermo Fisher Scientific). 1ug of total RNA was used for the construction of sequencing libraries RNA libraries were prepared for sequencing using the kits NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (Cat#E7770), following manufacturer´s instructions and 7-8 cycles of amplification