title: GSM8316164: Input pool, technical replicate 1; Escherichia coli; OTHER
library_construction_protocol: Plasmid purification were done with QIEprep Spin Miniprep (Qiagen) following instructions for large vector (>10kb). PCR amplification (25 cycles) were done with Phusion+ high fidelity polymerase (NEB). PCR products were gel purified (QIAquick PCR, Qiagen). Illumina adaptaters were added (PCR-free) following provider workflow (Novogen).