title: WGS of urothelial carcinoma: Sample WCM_IV_G_016_S02
design: Whole genome sequencing (WGS) libraries were prepared using the TruseqDNA PCR-free Library Preparation Kit (Illumina) in accordance with the manufacturer's instructions. Briefly, 1ug of DNA was concentration normalized and sheared using a Covaris LE220 sonicator (Adaptive Focused Acoustics). DNA fragments underwent bead-based size selection and were subsequently end-repaired, adenylated, and ligated to Illumina sequencing adapters. Final libraries were evaluated using fluorescent-based assays including qPCR with the Universal KAPA Library Quantification Kit and Fragment Analyzer (Advanced Analytics) or BioAnalyzer (Agilent 2100). Libraries were sequenced on an Illumina HiSeqX sequencer (HCS 3.3.76; RTA 2.7.6) at 2 x 150bp cycles using v2.5 reagents.