title: GSM7785101: 21s004505; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using miRNeasy Tissue/Cells Advanced Micro Kit (Qiagen). 1.5 ug of total RNA was used for the construction of sequencing libraries Standard-specific mRNA sequencing (mRNA-seq) libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA). Standard miRNA libraries were generated using the NEXTFLEX small RNA-seq kit v3 (Perkin Elmer, Waltham, MA, USA)