title: GSM7138257: PolyA_RNA-seq_Ctrl_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: HeLa cells were transfected in 15-cm plates as above with gene-specific gRNAs alone or a mix of gene-specific gRNAs for APA experiments. A quarter of transfected cells were used to extract total RNA (Trizol). Half a µg of total RNA was used to prepare polyA+ RNA libraries, using NEBNext Ultra II directional RNA library prep kit with NEBNExt PolyA mRNA Magnetic Isolation module.