title: GSM7504670: U2OS, treat with Etopside , rep4, batch2; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq libraries were generated from approximately 100,000 cell nuclei with the TruePrep® DNA Library Prep Kit V2 for Illumina (Vazyme TD501) ATAC-seq was performed as described previously with TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme TD501). Briefly, 50,000 cells were collected at 500×g for 5 min and resuspended in 50μl cold ATAC-seq lysis buffer and incubated on ice for 10min. Following centrifugation at 500×g at 4°C for 5 min, cells were pelleted and resuspended in 50μl transposition mix and then incubated at 37°C. The reaction was purified with VAHTS DNA Clean Beads and PCR amplified for 15 cycles. Purified ATAC-seq libraries were sequenced on the NovaSeq platform.