title: GSM8317780: Epcam Group 2 Hashtags; Mus musculus; RNA-Seq
library_construction_protocol: The lamina propria mononuclear cells (LPMC), stromal, and epithelial cells were sorted by FACS and were individually hash-tagged per mouse group and cell type. Then, the single cell suspensions were obtained and applied to the 10x Genomics workflow for cell capturing and scRNA gene expression (GEX) and CITE-Seq library preparation using the Chromium Single Cell 5' Library & Gel Bead Kit version 2 (dual index) and the Single Cell 5' Feature Barcode Library Kit (10x Genomics). After cDNA amplification, the CITE-Seq libraries were prepared separately using the Dual Index Kit TN Set A while, the final GEX libraries were obtained after fragmentation, adapter ligation, and final Index PCR using the Dual Index Kit TT Set A. Qubit HS DNA assay kit (Life Technologies) was used for library quantification and fragment average sizes were determined using the Fragment Analyzer with the HS NGS Fragment Kit (1-6000bp) (Agilent).