title: GSM6599048: GM12878_4cells; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Single cells were isolated with the digital microfluidics chip. After cell lysis, genomic DNA were obotained for methylome libraries construction. All of the following procedures are successively performed on the digital microfluidics chip: single-cell isolation; release of genomic DNA; spike unmethylated λ-DNA; digestion of the genome by MspI enzyme; repair and addition of an A-tail to the digested fragments; ligation of T-tail adapters; and bisulfite conversion of the ligated fragments. Subsequently, sufficient amplifiable DNA is purified and recovered from the chip, and sequencing libraries are generated after PCR amplification and fragment selection.