title: GSM8190135: CD8 positive sorted CAR T-cell (Replicate 2), irradiated (RT, 8 Gy) from mouse Lung tumor; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells following the Trizol Reagent User Guide (Thermo Fischer Scientific). Extracted RNA were quantified using Qubit 2.0 Fluorometer and RNA integrity was checked using Qubit 2.0 Fluorometer. SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification, and Illumina Nextera XT library was used for library preparation. Low count RNA-seq