title: GSM7675104: smRNA IP_Col0_C1; Arabidopsis thaliana; miRNA-Seq
library_construction_protocol: Total RNA was extracted using Tri-Reagent according to the manufacturer's instructions. For AGO1-loaded sRNA samples, AGO1-IPs were performed as described above from 1g of 1-week-old Arabidopsis seedlings grown on MS-agar plates and following similar HS and recovery treatments. AGO1-loaded sRNAs were then extracted by adding Tri-Reagent directly on the magnetic beads and extraction of RNA was performed according to the manufacturer's instructions. RNAs were precipitated O/N at -20°C in 2 volumes of isopropanol + 40 μg glycogen as described above. Small RNA seq libraries were generated using RealSeq v2 kit (Barberán-Soler et al., 2018), following manufacturer's instructions, with 100ng as initial input and 15 cycles PCR amplification.