title: GSM8190543: mCMs biological replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Collagenase II dissociated hiPSC-CMs on day 15 of cardiac differentiation and counted using a hemocytometer. Cells were resuspended in PBS + BSA (0.04%). Libraries were generated using the Chromium platform (10x Genomics) with the Next GEM Single Cell 3ʹ Reagent Kit v3.1, using an input of 1 million cells per mL. Gel-Bead in Emulsions (GEMs) were generated on the sample chip in the Chromium controller. Barcoded cDNA was extracted from the GEMs by Post-GEM RT-cleanup and amplified for 12 cycles. Amplified cDNA was then fragmented and subjected to end-repair, poly A-tailing, adapter ligation, and 10x-specific sample indexing following the manufacturer's protocol. Libraries were quantified using the TapeStation (Agilent Technologies). Libraries were sequenced using NextSeq 500 (Illumina) at Harvard Medical School.