title: GSM7211500: D24 Ctrl 24h; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were dissociated from kidney organoids using cold active protease into single cells and 10x Genomics sequencing was performed in the Advanced Genomics Core at the University of Michigan. Library was performed according to the manufacter's instructions  (single cell 3' v2 protocol, 10x Genomics for the NLW50 sample, single cell 3' v3 protocol, 10X Genomics for all other samples). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.