title: GSM8316820: HEK-2-input; Homo sapiens; RIP-Seq
library_construction_protocol: RNA from the HEKa was extracted using TRIzol. The ULI-MeRIP sample library was prepared using the SMARTer Stranded Total RNA-Seq Kit v2 following the manufacturer's guidelines. Input RNA, fragmented and IP-extracted, underwent fragmentation and reverse transcription into cDNA. Total RNA from embryos was fragmented according to the manufacturer's protocol. Subsequently, libraries of IP and input (or total RNA) samples were amplified through 15–19 cycles and 14–15 cycles, respectively. MeRIP-seq