title: GSM7785443: RBFox2_NT; Mus musculus; ChIP-Seq
library_construction_protocol: Sequential crosslinking was performed with the following steps: approximately 10^7 C2C12 cells were cross-linked with 0.2 mM dithiobis (succinimidyl propionate) (DSP) crosslinking solution at room temperature for 30 min, treatment with 3% final concentration of formaldehyde 15 min then quenched with 1/10 volume of 2.5 M Glycine, cells were washed and collected by centrifugation at 2,000 x g for 5 min at 4°C, flash-frozen in liquid nitrogen, and stored at -80°C. Then following our standard procedure, 6 μg of antibodies against RBFox2 (Bethyl Laboratories, A300-864A) was used for immunoprecipitation. Immunoprecipitated genomic DNA was resuspended in 25 μl of water. For DNA library construction, a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer's instructions. Bioanalyzer analysis and qPCR were used to measure the quality of DNA libraries including the DNA size and purity. Finally, DNA libraries were sequenced on the Illumina Genome Analyzer II platform.