title: DNA sequence reads of heligmosomoides bakeri: adult female
design: Adult H. bakeri nematodes collected from the intestine of infected Mus musculus and snap frozen. DNA was isolated using the MagAttract HMW DNA kit from Qiagen (ref. 67563) and followed manufacturer instructions. After isolation, the samples were cleaned up using a combination of kits Select-a-size Magbead kit (ref.D4084-3-10) and the Genomic DNA Clean and Concentrator 25 (ref.D4064) from Zymo. HMW DNA was confirmed using a FemtoPulse and converted to SMRTbell templates using the HiFi Express Template Prep Kit 2.0 (Pacific Biosciences, Menlo Park, C). SMRTbell libraries were size-selected on the BluePippin (Sage Science Inc., Beverly, MA). Sequencing was performed using the Sequel IIe (Pacific Biosciences, Menlo Park, CA) using sequencing chemistry 3.0 with 4-hour pre-extension and 30-hour movie time.