title: GSM7785434: Day 42 iPSC-derived chondrocytes + TGFb, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Tissues were digested in high-glucose DMEM containing 0.2% collagenase II and 0.1% dispase with gentle pipetting every 20-30 minutes.  Enzymatic digestion was observed in 6 well-plates, and digestion was terminated when aggregates were completely dissolved, for up to 2 hours.  Enzyme was inhibited with 20% FBS, and cells were filtered through 70 µm strainers and re-suspended in PBS supplemented with 0.04% high-quality bovine serum albumin at 1000 cells/µL for single-cell capture. Single-cell RNA sequencing was performed using 10X Genomics Chromium instruments.