title: GSM7094016: HRP-ER_HEK293T, small RNAs, control, post-enrichment, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs extracted through TRIzol reagent following manufacturer's instructions were diluted in H2O and digested by DNase I under 37°C for 15 min. Total RNAs were then purified with RNA Clean&Concentrator kit. The RNA integrity was detected by Fragment Analyzer and only samples with RQN>9.0 were kept. Small RNAs from digested samples were also purified with RNA Clean&Concentrator kit following the instructions. Approximate 300 μg purified total RNAs and 100 μg purified small RNAs from HEK293T cells, or 30 μg purified small RNAs from rat cortical neurons were used for affinity purification with streptavidin beads, the post-enrichment samples were purified by TRIzol Reagent. RNAs were reverse transcripted by SuperScript IV and were constructed in cDNA libraries by NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770) following the manuyfacturer's instructions.