title: GSM8059881: L1777_25_2; Solanum habrochaites; ATAC-seq
library_construction_protocol: Samples were prepared using sucrose sedimentation as previously reported but with slight modifications. Briefly, the young leaves of AC and LA1777 were collected under normal conditions or under cold stress for 6 h, respectively, and then ground to fine powder in liquid nitrogen. For each sample, 0.2 g of the frozen tissue powder was homogenized in prechilled 1 ml lysis buffer (15 mM Tris-HCl pH7.5, 20 mM NaCl, 80 mM KCl, 0.5 mM spermine, 5 mM 2-ME, 0.2% TritonX-100), and the nuclear fraction was purified as described47. The nuclei pellet was resuspended in 1 ml cold lysis buffer. For ATAC-seq and ATAC-qPCR, a nuclei aliquot (25 µl) was stained with DAPI (10 µl of 1 µg ml-1) and counted using a haemocytometer. Approximately 50000 nuclei were used for each ATAC-seq or ATAC-qPCR reaction.