title: GSM6465768: ERKi2173; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was harvested from lung organoids using RNeasy Kit (Quiagen, 74004) and quality control was performed on Agilent 2200 Tapestation with High Sensitivity RNA Screen Tape (Agilent, 5067-5579). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNS synthesis. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.