title: GSM8417615: Marfan1,  posterior, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Mitral valves were procured, rinsed in cold PBS, and sliced into small fragments around 1 mm in size. The minced tissue was incubated in DMEM with 1.5 mg/ml collagenase A, and 0.5 mg/ml elastase for 60 min at 37 °C while the tissue fragments were gently triturated using a pipette every 15 min. The digested solution was passed through a 70 μm filter and incubated with cell-impermeant viability dye (ThermoFisher Scientific, 65-0863-14 or 65-0865-14) for 20 min, washed, resuspended in 0.4% BSA/PBS for sorting using a LSR II (BD Biosciences) as described4. Single cells were processed using Chromium Controller (10X Genomics) according to the manufacturer's protocol. Library was performed according to the manufacturer's instructions (scRNA seq 3' v3 protocol, 10x Genomics).