title: +HBt_16s2
design: Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA GelExtraction Kit (Axygen Biosciences, Union City, CA, U.S.) according to the manufacturersinstructions and quantified using ABI StepOnePlus Real-Time PCR System (Life TechnologiesFoster City, USA) . Purified amplicons were pooled in equimolar and paired-end sequenced (PE250) on an Illumina platform according to the standard protocols