title: GSM8317878: LFDxC_XX_++_3; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were harvested from 6-week-old F1 hybrid mice (BL6 x CAST) and snap-frozen in liquid nitrogen. The GentleMACS Dissociator (program RNA_02_01) was used to homogenize tissues in 1 ml TRIzol per 50-100 mg sample. RNA isolation was conducted as described in the manufacturer's instructions (Invitrogen, TRIzol Reagent, Cat. #15596018) using 1 ml of the sample solution. RNA-seq libraries were constructed with 100 ng of RNA and Illumina's Stranded mRNA Prep Ligation Kit. The Agilent TapeStation System was used to assess the concentration and fragment length of the libraries. Sequencing was performed using a NovaSeq6000 50bp paired-end sequencing.