title: GSM7504653: WT3; Homo sapiens; RNA-Seq
library_construction_protocol: Extracting total RNA using trizol. Take a certain amount of Total RNA sample and use oligodT to obtain mRNA from the total RNA; MRNA interruption; Fragmented mRNA was added to random primers for cDNA strand synthesis; Synthesize cDNA double stranded; End repair, addition of "A" and splicing of double stranded cDNA; Connect the product for PCR reaction and purification recovery; Library quality inspection;