title: GSM7212180: CRISPR_screen.T10_rep3; Mus musculus; OTHER
library_construction_protocol: DNA of all time points and replicates  was extracted using the DNeasy Blood and Tissue kit (Qiagen). sgRNA cassettes were amplified from the collected genomic DNA and Illumina sequencing adaptors and sample barcodes were introduced in two consecutive PCR reactions, first with primers PCR1 and then with PCR2 primers, using Phusion polymerase (2U/µl) (Thermo Fisher Scientific). PCR products were purified with a QIAquick PCR Purification kit (Qiagen), molarity was assessed  with the Agilent 2100.