title: GSM8416800: Decompensated RV-3,SD rats, 8w-SuHx; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit (Qiagen). The tissues (no more than 30mg) were homogenized in 500µL Buffer RLT (containing 0.01% ß-mercaptoethanol). The homogenate was then mixed with 1 volume of 70% ethanol and was transferred to RNeasy Mini spin column and centrifuged. Next, the on-column DNase digestion was performed following the manufacture instruction. Following the DNase digesting steps, the column was washed with 700µL Buffer RW1 for once and 500 µL Buffer RPE for twice sequentially. Then the spin column was transferred to RNA collection tubes, and the RNA was eluted in 30µL RNase-free water. RNA concentration and quality were estimated using a NanoDrop Spectrophotometer. The sequencing library was constructed from the total RNA using the TruSeq RNA Sample Preparation Kit (Illumina) following the product manual.