title: GSM8285448: Mouse heart, F_DM 2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized under anesthesia and a piece of cardiac tissue was snap-frozen in liquid nitrogen. Total RNAs were isolated from pulverized frozen heart tissue using the PureLink RNA Mini Kit (Thermo Fisher). RNA was harvested using RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511). 5 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) following manufacturer's protocols.