title: GSM8009855: melanocyte, Ctrl2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted usingTrizol reagent following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit, high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library. RNA from each sample was used to generate RNA-seq libraries using a SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Japan). After quality inspection of the library, Illumina Novaseq™ 6000 was used for sequencing with a read length of 2 * 150 bp (PE150) for paired-end sequencing.