title: GSM7728240: P:C_0.55_Rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: On the 13th day after egg collection, Female flies were collected and snap frozen in Trizol. RNA was isolated using Trizol based extraction. RNA quality and quantity was checked on Bioanalyzer (Agilent Bioystems) as well as TapeStation (Agilent Biosystems) 100ng of Total RNA was taken for mRNA isolation, fragmentation and priming. The fragmented and primed mRNA was further subjected to first strand synthesis followed by second strand synthesis. The double stranded cDNA was purified using NEBNext Sample Purification Beads. The purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext Ultra II RNA Library Prep Kit for Illumina protocol.