title: DNA seq of Citrus clementina x Citrus x tangelo cultivar:fairchild PacBio 20kb SMRTbell 170401R209-C02_1
design: DNA was extracted from Fairchild Mandarin (Citrus reticulata) Accession No: PI 539508, Inventory No: RIV 2014 PL. Twenty-four hours before collection the tree was completely covered with brown paper bags to force the leaves to deplete starch storage. Leaves were harvested in the morning, and frozen in liquid nitrogen immediately after collecting. Intact leaves were shipped frozen to Arizona Genomics Institute for DNA extraction from nuclei. 20kb SMRTBell libraries were prepared per PacBio protocol including gTube size fraction and BluePippin size exclusion of fragments smaller than 20kb before constructing libraries for SMRTbell template construction. Sequencing on Pacbio RS II machine was performed at AGI following library construction.