title: Illumina NovaSeq 6000 paired end sequencing; Transcriptional coupling between Nrf2 and aB-crystallin in the lens and heart of zebrafish under proteostatic stress
design: Transcriptional coupling between Nrf2 and aB-crystallin in the lens and heart of zebrafish under proteostatic stress
library_construction_protocol: adult zebrafish lens tissues were dissected The following mutant and transgenic fish lines were used: cryabavu612 (cryba-/-); cryabbvu613 (crybb-/-); nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. Gupta, T. and M.C. Mullins, Dissection of organs from the adult zebrafish. J Vis Exp, 2010(37). The following mutant and transgenic fish lines were used: cryabavu612 (cryba-/-); cryabbvu613 (crybb-/-); nrf2fh318. All animal procedures were approved by the Vanderbilt University Institutional Animal Care and Use Committee. RNA was extracted using a Trizol reagent with RNA clean up kit (Zymo R2050) according to the manufacturer's instruction.  Raw sequencing reads were obtained for the paired-end samples.