title: GSM6736476: HC_BC000047_SNDA_5_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated and treated with DNase (Qiagen) using the Arcturus Picopure method (Applied Biosystems), yielding approximately 7-8 ng RNA per subject. Total RNA was linearly amplified into 5~10 µg of double-stranded cDNA using the validated, precise, isothermal RNA amplification method implemented in the Ovation RNA-seq System V2 (NuGen) Sequencing libraries were generated from 500 ng of the double-stranded (ds) cDNA using the TruSeq RNA Library Prep Kit v2 (Illumina) according to the manufacturer's protocol. The cDNA was fragmented, and end repair, A-tailing, adapter ligation was performed for library construction.