title: Nanopore sequencing of BrdU-labeled T98G glioblastoma cells: temozolomide-treated
design: T98G glioblastoma cells were plated in DMEM/10%FBS.  24 hours post-plating, 20uM temozolomide (Selleck) or DMSO was added to the media.  24 hours after drug/DMSO addition, 100uM BrdU was added to the media.  24 hours after BrdU addition, cells were harvested and DNA was isolated using a Quick-DNA kit (Zymo).  DNA was prepared for sequencing using a Cas9 kit (Oxford Nanopore) with sgRNAs targeting the promoter of MGMT.  Individual libraries were each sequenced for 24h on an R9.4.1 flow cell on a minION mk1C device.