title: GSM6639474: Spleen TN cells from untreated mice, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with RNAiso PLUS (TAKARA). Samples were further cleaned using an NucleoSpin RNA Clean-up XS (MACHEREY-NAGEL). RNA-seq libraries were prepared with NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB), using Multiplex Oligos for Illumina (Dual Index Primers Set 1, NEB), following the manufacturer's protocol.