title: GSM8059746: D374Y week 4 HFD CD20 1; Mus musculus; RNA-Seq
library_construction_protocol: livers were solubilized in Qiazol lysis reagent using Tissuelyser (Qiagen) and extracted RNA was isolated to upper fraction by chloroform. Purification was performed with RNeasy mini kit including on-column DNAse treatment, using a Qiacube robot. RNA was selected using Poly(A) RNA Selection Kit (Lexogen) and sequencing libraries prepared with Lexogen QuantSeq V2. DNA fragments 200-800 bp for RNA-seq were selected.