title: 1 mA electroacupuncture treatment, biological replicate 2
design: We divided 20 rats into 4 groups (n = 5 rats each): a normal group that comprised healthy rats; a COPD group that underwent modeling and remained untreated; a COPD + electroacupuncture (EA) group that underwent modeling and received 1mA EA treatment; a COPD + electroacupuncture (EA) group that underwent modeling and received 3mA EA treatment. Rats in the EA group were treated with 1mA and 3mA EA. The treatment lasted 14 days, 20 minutes per day. To explore the underlying molecular mechanism and signaling pathways associated with the effect of EA in treating COPD rat models, the lung tissues of Normal group, COPD group and EA group were disserted and prepared for RNA sequencing.