title: GSM6519884: RE1KO,BRBseq,multiplexed; Homo sapiens; OTHER
library_construction_protocol: Total RNA was purified by using Rneasy mini (QIAGEN) from homogenized cells. 500 ng of total RNA for each replicate was reverse-transcribed, pooled, and purified in two columns of Monarch PCR & DNA Clean up kit, followed by second-strand synthesis and tagmentation