title: Illumina HiSeq 4000 paired end sequencing; Comparative transcriptomics of Burkholderia pseudomallei from soil media and infected host
design: Comparative transcriptomics of Burkholderia pseudomallei from soil media and infected host
library_construction_protocol: This study compared the expression profiles of B. pseudomallei from infected mice to B. pseudomallei cultivated in soil extract media. B. pseudomallei in vivo was created by infecting BALB/c strain mice spleens through the intraperitoneal route and harvesting the spleens on day 5 post infection. Total RNA was obtained from two different sources: 1) mouse organs and 2) bacterial cultures. For the mouse samples, the spleens of infected and non-infected mice were harvested and RNA extracted on day 5 after infection. Bacteria samples were grown in soil extract medium and harvested during the mid-log phase. Total RNA was extracted from mice spleen and cultivated bacteria using the Trizol method, followed by DNA contamination removal and RNA purification using the Qiagen RNAeasy kit. The TruSeq RNA Library Prep Kit was used to prepare an RNAseq sequence library.