title: GSM7426223: tRNA ShControl rep 1; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA samples were extracted with the TRIzol reagent (Takara, #9109) according to the manufacturer's instructions, and over 2µg total RNA was resolved on urea-polyacrylamide gels and recovered within a size window of 60-100 nt for tRNA The partially hydrolyzed tRNA fragments were converted to barcoded small RNA sequencing libraries using NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs, #E7330L).