title: GSM8059278: Uninfected wild type control, Mouse 4 [NI_WT4]; Mus musculus; RNA-Seq
library_construction_protocol: The right ventricle was perfused with 3 ml of RNAlater (Thermo Fisher Scientific, Waltham, MA), whole lungs excised and immersed in RNA later and then cryopreserved. Whole lung RNA was extracted (NucleoSpin RNA Midi, Takara Bio, Shiga, Japan) and RNA concentration and integrity was assessed by sample absorbance at 260 and 280 nm. RNA-seq was performed by Novogene using Illumina sequencers. Briefly,  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis.