title: GSM8241333: SG-UPF2KD-2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol. The upper phase was taken, and RNA was precipitated. Precpitated RNA was dissolved in dH2O. 1 µg of RNA was used for library construction and sequencing. Poly(A) RNA was enriched from total RNA using NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs E7490L). Libraries constructed using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolab E7420L), and NEBnext Multiplex Oligos for Illumina Dual Index Primers.