title: GSM7505265: CB980 top2-4; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was prepared from 2.5x108 yeast cells with Trizol (Invitrogen) and RNA extraction was performed according to the manufacture's description. Briefly, 1 ml of Trizol reagent was added to the cells and cell disruption was achieved through vortexing (2 minutes) in the presence of glass beads. Then, 0.2 ml of chloroform was added to the cell suspension and incubated for 2 minutes at room temperature. Samples were centrifuged at 12,000 g for 15 minutes at 4˚C and the aqueous phase was transferred to a fresh tube and mixed with 0.5 ml isopropanol. For RNA precipitation, samples were incubated at room temperature for 10 minutes and centrifuged at 12,000 g for 10 minutes at 4˚C. RNA pellets were then washed with 70 % ethanol, air-dried and dissolved in 50 µl RNase-free water. RNA-seq samples were prepared according to the manufacture's standard protocol (TruSeq Standard Total RNA Sample Prep Kit, Illumina).