title: RNA-Seq of Phytolacca americana: Ethanol treated 1h
design: RNA quality was first assessed with a bioanalyzer and mRNA was isolated from each total RNA sample using NEB poly(A) mRNA magnetic isolation module with the NEB Ultra II Directional RNA kit which uses oligo dT beads to capture the mRNA transcripts that have a polyA tail for sequencing. A total of 30 samples were sequenced on a NovaSeq S4 flowcell PE 2x150bp at a depth of ~67-83 million reads per sample.