title: GSM6514058: fPTS2_1, small-sample ATAC-seq, female; Mus musculus; ATAC-seq
library_construction_protocol: 2-5 tubules were collected for either RNA extraction (Direct-zol RNA MicroPrep kit, Zymo Research) or ATAC-seq. Trizol-extracted RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMARTer V4 Ultra Low RNA Kit (Takara Bio). cDNA was generated with 10-12 PCR cycles. 1 ng of the resulting cDNA was “tagmented” and barcoded by using the Nextera XT DNA Sample Preparation Kit (Illumina) to generate sequencing libraries for high throughput sequencing. Libraries were purified by AmPure XP magnetic beads (Beckman Coulter), and quantified using a Qubit 2.0 Fluorometer (Thermo Fisher Scientific). An equal amount of index libraries were pooled and sequenced (paired-end 50 bp) on an Illumina platform.