title: GSM8098622: in vivo RS4.11 AZD1775 scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh bone marrow cells for day 5 sequentially treated ones, which were stained with Draq7 (BioLegend), hCD45 (BD Bioscience), and hCD19 (BioLegend), together with a PerCP/Cyanine5.5 anti-mouse CD45 (BioLegend). Approximately 20.000 alive/mCD45-/hCD45+/hCD19+ cells were sorted into a PBS (GE Healthcare Life Sciences) + 2% FBS (Thermo Scientific) coated tube and prepared for sequencing using the 10x Genomics platform at the Center for Translational Genomics, Lund University. Chromium NEXT GEM Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry Dual Index)