title: GSM8010921: leaf, CUT&Tag, rep1; Oryza sativa Japonica Group; OTHER
library_construction_protocol: 5 μg purified genomic DNA was fragmented into size ranging from 100-500 bp. Fragmented DNA was denatured in Z-DNA reconstitution buffer (20 mM sodium citrate buffer, pH 7.2, 4M NaCl, 25% ethylene glycol, 250 µm Mn2+ and 0.7 M Mg2+) at 95℃ for 8 min, then slowly cooled down to room temperature for Z-DNA re-association. For Z-DNA CUT&TAG, the detailed procedures were exactly followed according to the manual of the CUT&TAG kit (Cat#: TD904-01, Vazyme) produced by Vazyme. ZIP-seq,CUT&TAG