title: GSM7136176: Mesenchymal stem cell, GFP-overexpression; Homo sapiens; RNA-Seq
library_construction_protocol: The Nascent RNA was extracted from cells according to the Hirabayashi et al. (2019). The cDNAs were synthesized from nascent RNA using random primers. The ribose diols in the 5' cap structures of RNAs were oxidized, and then biotinylated. The biotinylated RNA/cDNAs were selected by streptavidin beads (cap-trapping). After RNA digestion by RNaseONE/H and adaptor ligation to both ends of cDNA, double-stranded cDNA libraries (CAGE libraries) were constructed.