title: GSM7135785: DU145_OE_eGFP_rep1_ATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 live DU145 cells expressing GFP or HOXB13 were lysed in 50 ul of cold lysis buffer (10mM Tris-HCl, pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% NP-40, 0.1% Digitonin, and 0.1% Tween-20) on ice for exactly 3 mins. The intact nucleus was tagmented with 2.0 ul of Tn5 Transposase (Cat # FC-121-1030, Illumina) and incubated at 370C on a thermomixer at 1000 RPM. The DNA was purified by the Minielute reaction cleanup kit (Cat # 28206, Qiagen). The DNA was amplified with unique barcoded primers and subjected to sequencing using Illumina Novaseq with a minimum of 30M reads for each sample.