title: Illumina NovaSeq 6000 paired end sequencing; RNAseq of L cells from Gcg-Venus HFD-STZ mice
design: RNAseq of L cells from Gcg-Venus HFD-STZ mice
library_construction_protocol: Ileal epithelial cells were dispersed and Venus+ L cells collected by FACS One group of mice was fed Research Diet #D12450J for 10 wk (regular chow). One group of mice was fed Research Diet #12492 (high-fat) for 10 weeks with administration of streptozotocin (35 mg/kg) for 5 days in week 6. RNeasy PlusMicro with DNAse NEB Next Ultra2 Directional RNAseq with rRNA depletion