title: GSM8415688: WT_CTX3; Mus musculus; RNA-Seq
library_construction_protocol: Mouse prostate tumor tissues were quickly excised and homogenized for RNA extraction. RNA was extracted using TRIzol RNA isolation reagent (Invitrogen, Cat n=15596026), followed by RNase-Free DNase set (Qiagen, Cat n=79254) treatment, following manufacturers' guidelines. RNA integrity was checked with Agilent 2100 Bioanalyzer system. RNA integrity was checked with Agilent 2100 Bioanalyzer system. NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (New England BioLabs Inc) and NEBNext poly (A) mRNA magnetic isolation module (New England BioLabs Inc) were used for cDNA synthesis. The libraries were sequenced using the NextSeq 2000 and P2 kit High (100 cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.