title: GSM6461721: Tcf12-WT-gGV-Rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Oocytes and embryos were collected from indicated genotypes (10 oocytes per sample). Each sample was directly lysed with 4.2 ul lysis buffer (0.2% Triton X-100, RNase inhibitor, dNTPs, oligo-dT primers, and 1:1000 ERCC spike-in) and immediately used for cDNA synthesis using the Smart-seq2 method as described previously (Picelli et al, 2014). Sequencing libraries were constructed from 500 pg of amplified cDNA using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD503) according to manufacturer's instructions. Barcoded libraries were pooled and sequenced on the Illumina NovaSeq 6000 platform.