title: Nanopore sequencing of BrdU-labeled glioblastoma coculture:DMSO treatment
design: Two glioblastoma cell lines (LN229 and T98G) were co-cultured in standard media.  24 hours after plating, 10uM dasatinib or DMSO was added to the media.  24 hours after drug/DMSO addition, 100uM BrdU was added to the media.  Cells were harvested 24 hours post-BrdU addition.  DNA was collected via Quick-DNA kit (Zymo) and prepared for Nanopore sequencing using a Rapid Barcoding Kit (Oxford Nanopore).  Library was sequenced for 24h on an R9.4.1 flow cell on a minION mk1C device.