title: RNA-seq of Akkermansia muciniphila in synthetic medium
design: Akkermansia muciniphila BAA-835 was grown in mucin-free synthetic medium to an OD600 of 0.4. Cells were treated with Qiagen RNAprotect Bacteria Reagent and RNA was isolated using a Qiagen RNeasy Mini Kit. RNA quality was checked with an Agilent Bioanalyzer instrument and ribosomal RNA was removed using Ribominus Bacteria (Thermo). Sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit (NEB) and sequencing was performed at the Duke Sequencing Core Facility.