title: 16S rRNA amplicon sequencing of net syndrome young peanuts Healthy peanuts
design: Peanut pod tissue total DNA was extracted from 0.1 gr frozen tissue samples using the GeneAll kit (cat 114-150) DNA isolation kit, PCR amplification of 16S rRNA gene was performed using 515F and 806R primers and ITS ampilfication was performed using CS1_ITS1F and CS2_ITS2R primers. Pooled libraries, with a 20% phiX spike-in, were sequenced on an Illumina MiSeq. De-multiplexing of reads was performed on instrument. Library preparation and pooling were performed at the University of Illinois at Chicago Sequencing Core (UICSQC.)PCR amplicon was sequenced using Illumina MiSeq.