title: GSM7674048: CTRL F2 [RA4]; Mus musculus; RNA-Seq
library_construction_protocol: Right atria were dissected from TBX5 removed and control 6‐8 week old mice. Tissues were mechanically homogenized in TRIzol Reagent (Invitrogen), chloroform was added, aqueous phase was isolated, ethanol was added, and then RNA was isolated using RNeasy Mini Columns. Overall, 12μg of total RNA for each sample was treated with the mouse GlobinClear kit (Ambion) to remove the majority of globin RNA. Libraries were prepared from this RNA starting with 1μg per sample and using the mRNA‐seq Sample Prep Kit (Illumina) as per recommended instructions. Cluster generation and sequencing were performed as per standard Illumina HiSeq2500 Rapid Run Mode protocols to generate single-end reads of 51bp. A single pool of libraries was sequenced in two individual lanes of the HiSeq2500 2‐lane flow cell. Barcoded Libraries were prepared according to Illumina's instructions (2013) accompanying the TruSeq RNA Sample prep kit v2 (Part# RS‐122‐2001). Libraries were quantitated using the Agilent Bio‐analyzer (model 2100) and pooled in equimolar amounts. The pooled libraries were sequenced on the HiSeq2500 in Rapid Run Mode following the manufacturer's protocols (2013). Total RNA-seq (Ribozero purification removed only ribosomal RNA, remaining total RNA was sequenced) of 50bp