title: GSM8098887: WL-COL-MOCK2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNAiso Plus (Takara) kit. RNA quality control was performed by gel electrophoresis. Three biological replicates were independently prepared throughout the processes, from the induction of seed germination to the preparation of RNA-seq libraries. Generation of RNA libraries using Illumina TruseqTM RNA sample prep Kit followed the manufacturer's instructions. The average RNA fragment was 300 bp and a 15-cycle PCR amplification was carried out with the primer mixture provided in the kit. Libraries were sequenced using an Illumina Hiseq4000 instrument with 2× 150-bp paired-ends reads by Majorbio.