title: GSM8010215: MethylC-seq_Cnt-1_IBM1-ox-L2; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: genomic DNA was isolated from leaf tissues using the DNeasy Plant Mini Kit (Qiagen), RNA was isolated from leaf tissues using the Qiagen Plant Mini Kit MethylC-seq libraries were constructed using the MethylC-seq protocol (Urich et al., 2015). Briefly, genomic DNA was sonicated to around 200 bp using a Covaris S-series focused ultrasonicator, and end-repaired with an End-It DNA end-repair kit (Epicentre). The end-repaired DNA was subjected to A-tailing using Klenow 3′–5′ exo− (NEB) and ligated to methylated adapters using T4 DNA ligase (NEB). The ligated DNA was treated with sodium bisulfite reagent using the EZ DNA methylation-Gold kit and amplified using KAPA HiFi uracil + Readymix Polymerase. RNA-seq libraries were constructed using Illumina TruSeq Stranded RNA LT Kit (Illumina) following the manufacturer's instructions with limited modifications.