title: WGS of Serrasalmus rhombeus: PacBio technology
design: DNA was extracted using a phenol/chloroform protocol and gDNA quality and purity was assessed using spectrophotometry, fluorometry and capillary electrophoresis. Additional purification steps were performed using AMPure PB beads (PACBIO). Library preparation and sequencing were performed according to the manufacturer's instructions "Procedure & Checklist Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0". 15g of DNA was purified then sheared at 15kb using the Megaruptor3 system (Diagenode). Then 10g was used for SMRTbell Express Template prep kit 2.0. A size selection step using a 10kb cutoff was performed on the BluePippin Size Selection system (Sage Science) with 0.75% DF Marker  S1 High Pass 15-20 kb. Using Binding kit 2.0 kit, sequencing kit 2.0 and primer V2, library was sequenced by diffusion loading onto 2 SMRTcells on Sequel2 instrument at 60pM with a 2 hours pre-extension and a 30 hours movie.