title: Human plasma; 45-year-old male with 7 years of disease
design: 1g genomic DNA was randomly fragmented by Covaris, The fragmented DNA was selected by Agencourt AMPure XP-Medium kit to an average size of 200-400bp. The selected fragments were through end-repair, 3 adenylated, adapters-ligation, PCR Amplifying and the products were recovered by the AxyPrep Mag PCR clean up Kit. The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The qualified libraries were sequenced on BGISEQ-500 platform (BGI-Shenzhen, china).