title: nicotiana benthamiana: mutant reseq
design: Genomic DNA was extracted from 15 mg of freeze dried leaves using DNeasy Plant Mini Kit (Qiagen catalog number 69104). DNA concentration was estimated using Quanti-it Picogreen ds DNA (Thermo Fisher catalog number P7589), 260/280 and 260/230 ratios were determined using Nanodrop (Thermo Scientific). Sample integrity and purity were detected by agarose gel electrophoresis . To prepare the library 1 microgram of DNA was used. The protocol for library preparation consists in genomic DNA fragmentation, fragment selection, end repair  and adaptor ligation, PCR , circularization, library validation and sequencing by DNBSEQ-G400