title: GSM8416787: Recql4 DKO rescue, day 0; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was extracted from the cell pellets using the Gentra Puregene kit (Qiagen). The extracted DNA was quantified on a Nanodrop spectrophotometer. DNA libraries were generated by PCR amplification of the integrated sgRNA constructs (as described in https://portals.broadinstitute.org/gpp/public /resources/protocols). DNA-seq of PCR amplified integrated sgRNA