title: GSM8145544: BT245 cells transduced with PRMT targeting shRNA TRCN0000303446 - H3K27ac immunoprecipitation; Homo sapiens; ChIP-Seq
library_construction_protocol: Approximately 5-10 million cells were fixed in 1% formaldehyde, after which cells were lysed. Nuclei were sonicated at low energy to fully isolate them from the cytosol. Nuclei were then sonicated at high energy in a Bioruptor Plus (Diagenode) to yield DNA fragments of 200-600 bp. Sequencing libraries of genomic DNA were prepared using the TruSeq Library Preparation Kit v2 (Agilent)