title: GSM8241721: CART_rep3; Mus musculus; RNA-Seq
library_construction_protocol: For in vivo comparison of CAR-T cells and tumors, mice were engrafted with CR or GPR65 KO tumors and treated with CAR-T cells 7 days later. Spleens were harvested 4 days after CAR-T treatment and RFP+ tumor cells and Thy1.1+ CAR-T cells were isolated by FACS. For total RNA-seq, library was constructed from RNA using Illumina TrueSeq stranded mRNA library prep kit and sequenced using the HiSeq or NovaSeq platforms (2 × 101-bp paired-end reads)