title: RNA-seq of Prionotus evolans: developing tail before separation
design: Total RNA was extracted from cells using the Qiagen RNeasy Plus Universal Micro Kit following manufacturers instructions (Qiagen). The SMARTSeq HT Ultra Low Input Kit was used for full-length cDNA synthesis and amplification (Clontech), and Illumina Nextera XT library was used for sequencing library preparation.