title: GSM8397054: ARPE-19 ATAC-seq, biological rep 2; Homo sapiens; ATAC-seq
library_construction_protocol: The tissue were re-suspended in cold PBS according to ATAC-Seq protocol. Transposition was performed at 37℃ for 60 min with gentle shaking. DNA was purified with the Qiagen DNA miniElute column and amplified for 16 cycles with indexed S5 and N7 primers. The final library was size-selected for products between 200-600 bp using the AMPure XP bead and subjected to pair-end sequencing (PE150) on a Novaseq6000 platform.