title: Autologous sera/stool: IgG bound
design: DNA Extraction from bacterial pellets was carried out in two stages: First stage involved lysing of the samples in Lysis Matrix E tubes on the Precelleys Tissue Homogenizer followed by transferring the supernatant into a deep well 96-well plate. Second stage consisted of DNA isolation from the supernatant, obtained from the previous stage, using Qiagen MagAttract PowerMicrobiome DNA/RNA EP Kit on Eppendorf automated liquid handling system. Dual-index amplification and sequencing approach was taken to assess the composition of microbial communities from the given samples using the V4 region of the 16S ribosomal RNA gene (16S rRNA) on the Illumina MiSeq Platform. 16S V4 libraries were prepared using 5l DNA as starting material in a 96-well PCR plate proceeding with PCR setup and cleanup using AMPure XP Beads (1:1 ratio).