title: GSM8416986: GSC1914 cells, shControl_1; Homo sapiens; RNA-Seq
library_construction_protocol: Lenti-X-293T cells cultured in DMEM media were transfected with lentivirus packaging plasmids (pMD2.g and psPAX2), along with either control or SUV39H1 shRNA vectors (Sigma-Aldrich Mission shRNA, #1 TRCN0000285355 and #2 TRCN0000275322), or pHAGE PGK-GFP-IRES-LUC-W (Addgene, cat# 46793) using the jetPRIME transfection reagent (Polyplus, cat# 101000001). After 24 hrs, the DMEM media was replaced with GSC culture media, and the cells were incubated for an additional 48 hrs at 37°C to produce lentivirus. The supernatant containing the lentivirus was harvested and saved in -80°C. For lentivirus infection, GSCs (4 x 10^5 cells per well in a 6-well plate) were exposed to the lentivirus for 18 hrs, followed by a media change to GSC culture media and a further 48 hrs incubation. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.