title: GSM7310380: LSK, ADT- and HTO-derived cDNA; Mus musculus; OTHER
library_construction_protocol: Mice aged ~6 months were subjected to iron chelator (Deferoxamine, 50 mg/kg) or vehicle control (HBSS) daily regimens for consecutive 7 days, repeated every 4 weeks for a total of 13 months. lineage depleted bone marrow cells were stained for 30 min with antibodies for lineage, Sca-1, c-Kit, TotalSeq C cocktail and sample-specific hashing antibodies. Stained cells were then sorted on Moflo Astrios EQ (Beckman Coulter), and approximately 20,000 Lin-Sca1+ckit+ (LSK) cells were acquired per sample. Single cell CITE-seq libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kit v2 (10x Genomics, Pleasanton, CA, USA). FACS-sorted LSK cells from bone marrow were stained and pooled in equal amounts prior to chip loading. Libraries were constructed according to manufacturer instructions. Libraries were purified and paired-end sequenced using NovaSeq 6000 S4 Reagent Kit v1.5 with 150 cycles (Illumina, San Diego, CA, USA).