title: GSM7673267: Subject CC0710 acute-omicron; Homo sapiens; RNA-Seq
library_construction_protocol: Blood was collected into Tempus Blood RNA tubes (Applied Biosystems) and the RNA was extracted using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus Blood RNA Tubes (ThermoFisher Scientific). RNA quality was assessed using a TapeStation 4200 (Agilent) and then 200 nanograms of total RNA was used as input for cDNA synthesis and library preparation using the KAPA RNA HyperPrep kit with RiboErase (HMR) Globin (Roche) according to the manufacturer's instructions. Libraries were validated by capillary electrophoresis on a TapeStation 4200 (Agilent), pooled at equimolar concentrations, and sequenced with PE100 reads on an Illumina NovaSeq 6000, yielding ~60 million reads per sample on average.