title: GSM7135733: E14-Mettl3-KO paused, time 3h, rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from equal number of ESCs (5x10^5 cells),RNA extraction using phenol-chloroform extraction, as per manufacturer's instructions. RNAs were treated with 10mM iodoacetamide and ethanol precipitated. Library preparation was done with Quant-seq 3′-end mRNA library preparation (Lexogen) using 100ng RNA.