title: RNASeq of Pacific herring liver VEN infection
design: Tissue samples were collected at days 10 and 34 from two temperatures, with control and VEN infected samples from each timepoint and temperature (n=6). Total RNA wwas extracted using a Direct-zolRNA Miniprep Kit TRI Reagent protocol (Zymo Research). Tag-seq libraries were prepared using the NEBNext Ultra II Direction RNA Library Prep Kit. Final libraries were sequenced as single-end 750 bp reads across two lanes of an Illumina NextSeq500. All libraries were sequenced in both lanes, and files concatenated prior to analysis