title: RNA-Seq of human: HD:MB03 cells
design: For sequencing analyses, HD:MB03 cells were exposed to 10 nM triptolide and total RNA was extracted with TRIzol. RNA quality was determined using an Agilent 2100 Bioanalyzer prior to performing global gene expression analysis by RNA sequencing (RNAseq). TruSeq Stranded Total RNAseq Library Prep kits (Illumina) were used to convert total RNA to cDNA libraries, which were then sequenced in a Genome Analyzer IIx system (Illumina).