title: GSM8241479: Batch 1, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Urine samples were collected in the morning of each patient. Once the samples were obtained, they were immediately transported on ice to the laboratory for processing. 150-200ml urine were collected and divided into 50ml centrifuge tubes for centrifugation (1000 rpm for 5min). The urine sediment was suspended in PBS and treated by ACK lysing buffer (Gibco A1049201) to remove erythrocyte.  After cleaning twice with PBS (1000 rpm for 5min), living cells were enriched with Dead cell removal kit (Miltenyi, 130-091-286) according to the manufacturer's protocol. Then, after labeling the collected cells, mixed samples were carried out and sequenced using the BD single cell sequencing platform. All steps were performed on ice or at 4 degrees. Microwell-based scRNA-seq was performed according to the manufacturer's instruction provided by BD Biosciences.