title: GSM7025551: KO_Ero1a; Mus musculus; RNA-Seq
library_construction_protocol: Preparation: We collected tumour grafts from wild type mice (n=5) and Ero1a KO mice (n=5). The samples were washed with 2% (v/v) FBS-PBS. Filtration of   tumour lysates through a 100 μm filter to form single-cell suspensions. After quantifying the cell number, adjusting the concentration to 800-1200 cells/μl, and ensuring the cell activity > 85%, single-cell suspensions from these specimens were subjected, typically within an hour after the acquisition, to single-cell RNA sequencing (scRNA-seq) using the 10X genomics platform to construct the single-cell library of samples. Follwed by v3 protocol of chemistry of the 3' scRNA-seq 10X genomics platform