title: GSM7847362: WT Control 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was ioslated from snap-frozen colonic tissue using Qiazol Reagent and Qiagen RNeasy kit (cat no. 74106) was followed as per manufactors instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.