title: GSM7728120: wildtype plate 2; Danio rerio; RNA-Seq
library_construction_protocol: Unfixed ventricles were dissociated by Collagenase and TrypLE Express treatment to prepare single cell solutions. Cells were subsequently FACS sorted based on nppa:mCitrine expression in 384 well plates, each well containing 1 cell, containing Vapor-Lock oil with CEL-Seq2 barcoded primers, spike-in mRNA and dNTPs (Single Cell Discoveries). Plates were spun down and frozen at -80oC until ready for library prep. Single-cell sequencing libraries were prepared by Single Cell Discoveries (Utrecht, The Netherlands) using the SORT-seq protocol . The CEL-Seq2 protocol was used for library prep (Hashimshony et al., 2016)). Illumina sequencing libraries were prepared with the TruSeq small RNA primers (Illumina) and paired-end sequenced at 75 bp read length on the Illumina NextSeq platform. In total seven 384-well plates were sequenced containing one cell per well of which three were obtained from wildtype cells and four from hmga1a mutant cells.