title: GSM8317795: Input_Swi6_snf22Δ_rsc1Δ_Rep2; Schizosaccharomyces pombe; ChIP-Seq
library_construction_protocol: Cells were crosslinked using 3% PFA at room temperature or 18°C for 30 min. Cells were spun at 3000rpm for 5 min washed and pellets were lysed using glass beads and lysis buffer (50mM Hepes/KOH, 140mM NaCl, 1 mM EDTA, 1% Triton X100, 0.1% DOC plus protease inhibitors). Cell lysates were sheared using a Bioruptor-300 (Diagenode) to an approximate size of 200–700 bp. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's protocol and analyzed using an Agilent 4200 TapeStation system (Agilent).