title: GSM6792301: INA6 multiple myeloma cells co-cultured with HS5 stromal cells for 72 hours; Homo sapiens; ATAC-seq
library_construction_protocol: For each condition, 50,000 CD138+ multiple myeloma cells were aliquoted and prepared in duplicates as previously reported.46 Cells were lysed for 10 minutes at 4°C in lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-360). After lysis, the pellets were subject to a transposition reaction (at 37°C for 60 minutes) using the TD buffer and transposase enzyme (Illumina Nextera DNA preparation kit, FC-121–1030). The transposition mixture was purified using a MinElute PCR purification kit (Qiagen). Library amplification was performed using custom Nextera primers and the number of total cycles determined by running a SYBR-dye based qPCR reaction. Amplified libraries were purified using a PCR purification kit (Qiagen) and sequenced.