title: RNA-seq of Capsicum annuum Dempsey: Shoot1
design: For understanding the mechanism of de novo shoot organogenesis, we extracted total RNA from leaf, callus, and callus-derived emerging shoot tissues. The libraries were prepared for 75 bp paired-end sequencing using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA; RS-122-2101). Sequencing of the cDNA libraries was performed using an Illumina MiSeq platform (Illumina, San Diego, CA, USA).