title: GSM7504460: HSA-000027442,WT,ctrl; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from frozen brain powder using the Qiagen RNeasy plus kit (Qiagen 74034) and RNA quality and quantity was assessed with a 4200 TapeStation System (Agilent) Libraries were generated using the QuantSeq 3' mRNA-seq Library Prep Kit FWD for Illumina (Lexogen A01173) with the UMI second strand synthesis module in order to identify and remove PCR duplicates, following the protocol defined by the manufacturer. Library quantity and quality were assessed with the 4200 TapeStation system (Agilent). Libraries were pooled in equimolar ratios for sequencing.