title: RNA-Seq of StCLE4-oe roots on nitrogen-free media
design: Total RNA was extracted from samples using the RNeasy Plant Mini Kit (Qiagen, USA). Enrichment of mRNA was performed using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). The libraries were prepared using NEBNext Ultra II Directional (New England Biolabs, USA). For sequencing HiSeq PE Rapid Cluster Kit v2 and HiSeq Rapid SBS Kit v2 was used.