title: GSM8099030: KYSE450, shSOX2, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq analysis , equivalent numbers of cells from each group were used for preparation of RNAs. Total RNA was isolated from each sample using RNAiso Plus (TARAKA).The same amount of spike-in yeast RNAs (10%) was added to each RNA sample.  RNA quality was examined by gel electrophoresis and with Qubit (Thermo, Waltham, MA, USA). RNA libraries were constructed using VAHTS Stranded mRNA-seq Library Prep Kit for Illumina (Vazyme, China),and sequencing was carried out using the Illumina Novaseq 6000 instrument by the commercial service of Genergy Biotechnology Co. Ltd. (Shanghai, China).