title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70
design: RNA-seq of snrnp70 mutant zebrafish and its rescue by overexpression of cytoplasmic SNRNP70
library_construction_protocol: Embryos were collected and raised in Danieau's solution together with 2 μΜ 4-OHΤ. Treated embryos were screened for GFP fluorescence at 24hpf and sorted into the four genotypes. Total RNA from whole embryos was isolated from each genotype group at 28hpf using a RNeasy Mini kit and eluted in nuclease-free water. Total RNA was purified using Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold