title: Whole-genome sequencing of Planococcus sp.
design: Illumina sequencing: Genomic DNA was extracted from cultures using XYZ. DNA was fragmented using a Covaris M220 ultrasonicator to an average fragment length of ~400 bp. DNA size distributions were assessed using an Agilent 2200 Tapestation instrument. Illumina libraries were prepared using a Quantabio sparQ DNA library prep kit following the manufacturers protocol. Steps consisted of end repair and A-tailing, adapter ligation, and library amplification. After library amplification and pooling, libraries were size selected to a range of 300-600 bp using a Sage Science BluePippin instrument. The concentration of the final library pool was determined using a Kapa Library Quantification kit (Roche). Libraries were sequenced on an Illumina MiSeq instrument using a v2 500 cycle sequencing kit to generate 2x250 paired end reads. Nanopore sequencing: Nanopore libraries were prepared using the Oxford Nanopore Technologies Rapid Barcoding Kit following the manufacturers protocol. Libraries were sequenced on a MinION device using Flongle flow cells.