title: GSM7310320: ACLi_A6; Mus musculus; OTHER
library_construction_protocol: Cardiac fibroblasts were fixed with 0.1% formaldehyde for 1 minute, followed by quenching with 125mM glycine. After, chromatin was isolated according to protocol from Eipcypher Kit: 14-1048, with 0.01% digitonin to permeabilize cells. CUTANA™ CUT&RUN Library Prep Kit (14-1001) following manufacterers instructions. 5ng CUT&RUN enriched DNA was used as starting material for IP samples. Libraries were amplified using 14 cycles on the thermocycler. Libraries checked with Qubit for quantification and bioanalyzer for size distribution at Novogene.