title: GSM6736292: Hypothalamus tissue, control, rep1 [G1]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted by affinity chromatography using PicoPure RNA isolation kit (Thermo Fisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library construction. Directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.