title: GSM8009741: ΔORF heart snATAC-seq; Mus musculus; ATAC-seq
library_construction_protocol: Whole hearts were harvested from both wildtype and ΔuORF mice, which were at an age of 2 month (n = 3), and were fast frozen in liquid nitrogen. Nuclei were isolated from the frozen mouse heart tissue using the 10x Genomics single nuclei isolation kit, adhering to the manufacturer's instructions. We isolated nuclei from frozen mouse heart tissue and constructed single nuclei gene expression libraries and single nuclei ATAC libraries on the 10x Genomics Chromium System using the 10x Chromium Multiome kit. Each library was sequenced with ~200 million pair end reads (PE150) on Illumina NovaSeq 6000