title: GSM7675093: K18-hACE2 mice, 35, COVID-19 48 hours post infection; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were isolated and minced with forceps and scissors. They were further enzymatically digested with 2 mg/mL collagenase (Sigma) and 80 U/mL DNase I (Sigma) in 2 mL serum-free medium for 60 min at 37°C. Digested tissues were passed through a sterile 70 mm filter (Fisher Scientific) and 40 mm filter (Miltenyi Biotec) to generate a single cell suspension. After washing, cell suspensions were incubated with 2 ml of ACK Lysing Buffer (Cat #A1049201, Gibco) for 2 min and washed twice. 1x106 cells per condition were collected as whole lung single cell population. Cell numbers and viability were validated by Countess II (Thermo Fisher) prior to preparation of single cell RNAseq library.