title: Transcriptomes of subterranean mammals reveal that naked mole rats have distinctive cardiometabolic and genetic adaptations to their underground low-oxygen lifestyles
design: Transcriptomes of subterranean mammals reveal that naked mole rats have distinctive cardiometabolic and genetic adaptations to their underground low-oxygen lifestyles
library_construction_protocol: Heart was dissected and within 60 seconds placed in cryovial containing RNA later, and flash frozen in liquid nitrogen. Subsequently, samples were placed in the -80C freezer for storage. Total RNA extraction and analysis was performed commercially by BGI Tech solutions (Hong Kong). Oligo dT beads were used to enrich mRNA with a poly A tail. RNA fragmentation and first- strand cDNAs were generated using random N6-primed reverse transcription, followed by the second- strand cDNA synthesis. The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments. PCR amplification was performed to enrich the purified cDNA template.