title: GSM6460373: S8; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the BM cells of 4 different drug treatment groups (Group 1: Vehicle, Group 2: PD-901, Group 3: 5-azacitidine, and Group 4: Combo) using Qiagen RNeasy Plus Micro Kit. RNA with Agilent Bioanalyzer RIN of > 7 were selected for library preparation. Library preparation was performed using New England Biolabs Single Cell/Low Input RNA Library Prep Kit for Illumina. Paired-end RNA-sequencing (RNA-seq) was performed on Illumina HiSeq4000 by Novogene (project number: Davis-US-IU-12-mouse-EukmRNAseq-6G-WOBI-NVUS2022042753). Each group/condition consists of three matched biological replicates and ~30 million reads were obtained per sample.