title: GSM7984578: bone marrow, CCR1/2+ monocytes, resting transcriptome; Mus musculus; RNA-Seq
library_construction_protocol: CCR1/2+ monocytes (CD45+, CD11b+, Ly6Chi, Ly6G-, SiglecF-, CCR2+, CCR1+) were sorted from the bone marrow of REP mice using BD FACS Aria and collected into 1% BSA in PBS. Single cell libraries were generated using 10X Genomics Chromium NextGEM Single Cell 3' kit (v 3.1) and following the manufacturer's instructions.  Cells were added to the 10X Genomics NextGEM Chip G at a concentration between 700 and 1200 cells/µl, with a targeted cell recovery of 7000 cells. Cells were combined with Gel Beads-in-Emulsion (GEMs) on the Chromium Controller prior to lysis and reverse transcription. GEMs were then broken and cDNA amplification and fragmentation performed.  Post-fragmentation, the i7 sample index was ligated, and Illumina P5 and P7 adapters added. Sequencing was performed by Glasgow Polyomics on the Illumina NextSeq-2000 sequencer using a custom paired end sequencing run (28x90bp) to yield 250M reads.