title: Illumina HiSeq 3000 sequencing; Transcriptional profiling of non-lesional and lesional human skin of positive patch-test reactions to nickel at 24h, 48h, and 120h post allergen application
design: Transcriptional profiling of non-lesional and lesional human skin of positive patch-test reactions to nickel at 24h, 48h, and 120h post allergen application
library_construction_protocol: Donor-matched skin biopsies were taken from non-lesional (NL) and Lesional human skin of positive patch-test reactions to nickel at 24h, 48h, and 120h post allergen application. Biopsies were embedded in OCT cryo-embedding matrix, frozen in liquid nitrogen, and stored at -80Â°C until use RNA-seq reads were mapped to the human reference genome (GRCh38, build 81) using HISAT2 v. 2.0.4 (Kim et al., 2015). Total RNA was isolated with Blood & Tissue Kit (Qiagen). Illumina TruSeq Stranded mRNA protocol