title: NGS of chicken: 1_gamma_day0
design: RNA was extracted from splenocytes using the RNeasy Mini Kit (Qiagen) and then reverse transcribed to cDNA by 5'RACE with a SMARTScribe Reverse Transcriptase (Takara). cDNA was amplified in two semi-nested PCRs using an Advantage2 Polymerase (Takara).