title: GSM7135692: mutant_epidermis_1; Danio rerio; RNA-Seq
library_construction_protocol: The EGFP+DsRedx- progenitor cells for RNA-seq were sorted by BD FACSAria™ IIIu cell sorter. 30 cells per sample were sorted. cDNA library was prepared according to the standard Smart-seq2 protocol with minor modifications. Briefly, 30 cells per sample were sorted into the lysis buffer premixed with dNTP and oligo-dT primer. The whole lysate was proceeded to reverse transcription by SuperScript™ II Reverse Transcriptase (18064071, Invitrogen). The cDNA was then amplified with KAPA HiFi HotStart ReadyMix PCR Kit (50-196-5217, Roche) for 23 cycles and purified with the Ampure XP beads (A63881, Beckman). The cDNA library was prepared with TruePrep DNA Library Prep Kit V2 for Illumina (TD501, Vazyme).