title: GSM6640393: DROSHA-KO HCT116 cells transfected with pCK-DROSHA_belt_deletion plasmid repeat 3; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNAs were extracted using TRIzol reagent. The small RNAs (19–24 nt) from 10 μg of each total RNA sample were size-selected in a 15% urea-PAGE. The purified small RNAs were cloned using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB) to generate the small RNA libraries.