title: COI amplicon sequencing of Vespula germanica meconium sample: queen larva 6
design: COI amplicon sequencing was conducted on an Illumina NovaSeq Platform (Illumina, San Diego, USA) by Azenta Life Sciences (Suzhou, China). Amplicons cover 313 bp of the cytochrome c oxidase I (COI) gene. Automated cluster generation and 250 paired-end sequencing with dual reads were carried out following the manufacturers instructions. Double-end sequencing was then performed on both positive and negative reads, with the initial read joining. Sequences containing ambiguous bases (sequence of N) were filtered out, retaining only sequences > 200 base pairs.