title: GSM7674010: Sperm HFD-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared from Morula, Sperm and Round spermatids using the RNeasy mini kit (QIAGEN) according to the manufacturer's instructions. RNA purity, integrity and quality was examined by Bioanalyzer (Agilent Technologies) and Qubit (Thermo Fisher Scientific). Transcript Libraries were constructed using the Nextera Library Prep Kit (Illumina) according to the manufacturer's instructions and sequenced on an Illumina HiSeq 2500.