title: DNA seq of Citrus clementina x Citrus x tangelo cultivar:fairchild PacBio CCS 150522R080-E02_1
design: DNA was extracted from Fairchild Mandarin (Citrus reticulata) Accession No: PI 539508, Inventory No: RIV 2014 PL. Twenty-four hours before collection the tree was completely covered with brown paper bags to force the leaves to deplete starch storage. Leaves were harvested in the morning, and frozen in liquid nitrogen immediately after collecting. Intact leaves were shipped frozen to Arizona Genomics Institute for DNA extraction from nuclei. SMRTBell libraries were prepared per PacBio protocol at AGI and sequencing on Pacbio RS II machine