title: GSM8059935: WT, replicate 3, CB, bulkRNA; Mus musculus; RNA-Seq
library_construction_protocol: GC B cells were isolated from mice spleens at day 10 post-immunization using the Germinal Center B Cell (PNA) MicroBead Kit (Miltenyi Biotec,130-110-479). RNA was extracted using the RNeasy Plus Micro Kit (QIAGEN) according to the manufacturers protocol. RNA concentration was determined using the Qubit RNA High Sensitivity Kit (Thermo Fisher Q32855) and RNA integrity was assessed using High Sensitivity RNA ScreenTape analysis (Agilent 5067-5579) on the Agilent 4200 TapeStation system. mRNA-seq library preparation was performed using KAPA RNA HyperPrep Kit with RiboErase (HMR), following the manufacturer's protocol. Library size distribution and concentration were evaluated using the Agilent 4200 TapeStation system and Qubit dsDNA HS Assay Kit (ThermoFisher Scientific), respectively.