title: GSM8326620: N269 DPF2; Mus musculus; OTHER
library_construction_protocol: Zymo DNA clean up and concentration kit Approximately 5-10 million cells were crosslinked and then H3K27ac antibodies were used for DNA fragment capture. For ChIP-seq sequencing libraries were prepared from chromatin immunoprecipitated DNA samples (1 to 10 ng) using the New England Biolabs NEBNext Ultra II DNA Library Prep kit (cat#E7634L).  PCR-amplified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Libraries were normalized and pooled in preparation for Illumina sequencing. HiChIP libraries were prepared using Illumina Tagment DNA Enzyme and Buffer Kit.