title: GSM6736629: PBMC_perf_10X; Homo sapiens; RNA-Seq
library_construction_protocol: scRNA was extracted from 10,000 human PBMCs. A frozen stock of PBMC (isolated from whole blood with Histopaque and stored in FBS with 10% DMSO at -80°C) was thawed, washed twice with RPMI1640 complete media then kep in CO2 incubator for 4 hours. After this short incubation, cells were washed twice with DPBS with 0.1% BSA. Cell concentration was adjusted to 10,000 input cells to 10x Genomics Chromium system. Single cell sample preparation was done according to instruction of 10x Genomics 3' kit version 3.1. Library preparation was made accoding to instruction of 10x Genomcis's 3' kit version 3.1.