title: GSM7673464: Dp1Tyb, IsoL21, 1; Mus musculus; RNA-Seq
library_construction_protocol: E13.5 frozen embryonic hearts were placed in lysis buffer and homogenized using a cordless Pellet pestle (Sigma-Aldrich) followed by RNA extraction using RNAeasy micro kit (Qiagen) polyA enriched libraries usingNEBNext®Ultra™II Directional RNA Library Prep Kit for Illumina according to manufacturer's instructions. An average 25 million pair end reads per library (PE100) were generated on a NovaSeq6000 (Illumina).