title: GSM7673586: spike for sgIkzf1_Tpex_2; Mus musculus; ATAC-seq
library_construction_protocol: Cells were lysed in 50μl ATAC-seq lysis buffer (10mM Tris-HCl, pH7.4, 10mM NaCl, 3mM MgCl2, 0.1% IGEPAL CA-630 detergent) on ice for 10min. Resulting nuclei were pelleted at 500gfor 10min at 4 °C. Supernatant was carefully removed with a pipette and discarded. The pellet was resuspended in 50μl transposase reaction mix (25μl 2×TD buffer, 22.5μl nuclease-free water, 2.5μl transposase) and incubated for 30min at 37 °C. After the reaction, the DNA was cleaned up using the Qiagen MinElute kit The barcoding reaction was run using the NEBNext HiFi kit on the basis of the manufacturer's instructions and amplified for five cycles