title: Ion Torrent Proton sequencing; The analysis of transcriptional profiles of cybrid cells harbouring two pathogenic mtDNA variants associated with Leigh syndrome
design: The analysis of transcriptional profiles of cybrid cells harbouring two pathogenic mtDNA variants associated with Leigh syndrome
library_construction_protocol: Cultivated cytoplasmic hybrid cells were collected and washed with PBS. All experimental workflow was performed in two biological replicates.  Total RNA isolation with RNeasy Mini Kit (QIAGEN Science, MD, USA), according to the manufacturer's instructions. The integrity of the extracted RNA was evaluated by the RNA integrity number (RIN) within the Agilent 2100 Bioanalyzer system (Agilent, USA). Ribosomal RNA depletion was done with Low Input RiboMinus Eukaryote System v2 (Thermo Fisher Scientific, USA), by processing 500ng of total RNA from each sample. For cDNA library preparation we used the Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific, USA).