title: GSM8397981: MH258 cells, deltaCsrD mutant, rep1; Klebsiella pneumoniae; RNA-Seq
library_construction_protocol: Total RNA was isolated from each cell type (in biological duplicate) and purified using RNAeasy RNA extraction kit (Qiagen). RNA was then DNAse treated (TURBO) to remove all DNA RNA was submitted to the UChicago DFI Metagenomics Core for Illumina RNAseq Library Prep. Illumina compatible libraries were generated using NEBNext® Ultra™ II Directional RNA Library Prep Kit. Ribosomal RNA depletion (NEB) was used prior to library preparation.