title: GSM8319644: Achilles tendon, exercised 1 h and  sacrificed immediately, rep4; Rattus norvegicus; RNA-Seq
library_construction_protocol: Fresh Achilles tendon tissues from the right legs of the running and naïve control rats were immediately dissected and placed in RNALater™ (Beyotime) to prevent RNA degradation. The total RNA was extracted after the tissues were mechanically pulverized in TRIzol (Thermo Scientific). After verification of the RNA integrity by Qsep100 (BIOptic; RNA integrity number > 7.0), polyA mRNAs was purified from total RNA using mRNA capture beads (Vazyme, N401). Then the mRNA was reverse-transcribed to create a cDNA library. VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 (Vazyme Biotech, NR612-02) was used for library preparation. RNA sequencing was performed using the Illumina NovaSeq 6000 sequencer.