title: 16S rRNA of conjunctival sac microbiomeMixed dry eye
design: Conjunctival sac swabs were collected from 15 eyes with meibomian gland dysfunction and 13 eyes with mixed dry eye (MGD with ADDE). Isolated bacterial DNA from conjunctival sac swabs were analyzed with 16S ribosomal RNA (rRNA) gene amplicon sequencing. Alpha diversity analysis of microbial community diversity was performed by Chao8 index, Shannon index and Simpson index. Beta diversity was validated with principal coordinate analysis (PcoA) and bioinformatic analysis of relative abundances and their functional annotations was performed at the genus levels.