title: GSM7135723: E14-paused, Mycn-gRNA3, rep4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from equal number of ESCs (2x10^5 cells), with RNeasy Micro Kit with on-column DNase I digestion (QIAGEN). RNA was spiked with synthetic RNAs from the External RNAs Control Consortium (ERCC) Spike-in Mix1 (Thermo Fisher), with 2µl of 1:100 ERCC dilution added to 10µl of RNA, equivalent to ~1-2µg RNA. Library preparation was done using the NEBNext Ultra II Directional Library Prep Kit for Illumina with the mRNA Magnetic Isolation Module from 1µg RNA, per manufacturer's instructions (NEB).