title: RNAseq of pulldown with PIP2 of Nuclear fraction RNA of HeLa cells
design: RNA extraction from the nuclear fraction of HeLa cells followed by pulldown with phosphatidylinositol 4,5 bisphosphate (PIP2) coated-beads and recovery of bound RNA, for sequencing ribosomal RNA was removed. After RNA fragmentation, the first strand cDNA was synthesized, a second strand cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification