title: GSM7674033: Gas HFDt-1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was prepared from liver, GAS, and eWAT using the TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA purity, integrity and quality was examined by Bioanalyzer (Agilent Technologies) and Qubit (Thermo Fisher Scientific). Sequencing libraries were prepared by using the QuantSeq 3′ mRNA-Seq mRNA Library Prep Kit FWD for Illumina (Lexogen) with i7 indexes (Lexogen) according to the manufacturer's instructions. Libraries were sequenced on an Illumina HiSeq 2500.