title: WGS of cell line: CHLA266
design: Libraries were prepared using the TruSeq DNA PCR-free Library Preparation Kit (Illumina) with 1 g DNA input following Illuminas recommended protocol, with minor modifications as described. Intact genomic DNA was concentration normalized and sheared using the Covaris LE220 sonicator to a target size of 500 bp. After cleanup and end-repair, an additional double-sided bead-based size selection was added to produce sequencing libraries with highly consistent insert sizes. This was followed by A-tailing, ligation of Illumina DNA Adapter Plate (DAP) adapters and two post-ligation bead-based library cleanups. These stringent cleanups resulted in a narrow library size distribution and the removal of remaining unligated adapters. Final libraries were run on the Agilent TapeStation D1000 ScreenTape to assess their size distribution. The libraries were pooled together based on expected final coverage and whole genome sequencing was performed on the NovaSeq 6000 at 2x 150bp read length using v1.5 chemistry S4 reagents.