title: GSM7213758: TWIST1FV, 1h dTAG, CUT&RUN, CTCF; Homo sapiens; OTHER
library_construction_protocol: The CUTANA CUT&RUN (Epicypher) protocol v1.9 and reagents (concanavalin A beads, 21-1401 and pAG-MNase, 15-1016) were used with minor modifications: digestion was performed for 30 min on ice, and digestion supernatant was treated with 0.1% w/v SDS and 0.25 mg/ml proteinase K at 50°C for 1 h and then phenol-chloroform extraction was performed to extract DNA. Primary antibody incubations were performed overnight, and secondary antibody was used for mouse antibodies (TWIST1, ALX4, TCF3). Library prep was performed with modifications to the NEBNext Ultra II DNA kit as in dx.doi.org/10.17504/protocols.io.bagaibse.