title: GSM8146147: Post_0W_18_biol rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Trizol RNA extraction (SIGMA) followed by DNase I (NEB) treatment following manufacturer's instructions. 1 ug of RNA was ribodepleted using Hybridase thermostable RNaseH (Epicentre, H39500) and total libraries were prepared using NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 (PerkinElmer) as recommended by the manufacturer.