title: GSM7139013: heart; Danio rerio; RNA-Seq
library_construction_protocol: For each organ, six sex-balanced zebrafish were pooled together for the cell sorting and a total of 30000 DAPI-mpeg1.1-DsRedx+ cells were sorted into 5 μl collection buffer (4.5 μl 1% BSA/PBS + 0.5 μl RNaseOUT) using BD Influx high-speed Cell Sorter (BD Biosciences). scRNA-seq was carried out with a 10X Chromium single cell platform (10X Genomics). In brief, after the cell sorting, single-cell suspensions were prepared according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide and loaded onto the 10x Chromium Single Cell Controller to generate single-cell Gel Beads-in-emulsion (GEMs). Libraries were prepared using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3.1 according to the manufacturer's instructions. Library quality was assessed using an Agilent Fragment Analyzer System, followed by sequencing on Illumina Novaseq 6000 system (Illumina) for a targeting average depth of around 100,000 read pairs per cell.