title: GSM8098646: Pooled_PBEC_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: At 28 day of ALI, the apical compartment of the traswells were washes twice with 250ul of PBS. The cells were then trypsinised before centrifugation at 1600 x rpm for 5 min. Cells were collected into sterile 1 x PBS and viability of all six populations was confirmed by trypan blue staining (Sigma-Aldrich) and found to be at or above 92% viable. Cells from different individuals were pooled into multiplexed cell suspensions and subsequently demultiplexed after sequencing on the basis of genetic variants Chromium Single Cell 3ʹ v3