title: miRNA-seq of Juglans mandshurica Maxim.:protogyny male buds
design: Small RNA libraries were constructed using the TruSeq Small RNA Sample prep Kit from llumina. Libraries were enriched by PCR amplification with the addition of sequencing connectors and Index sections. Libraries were purified by gel electrophoresis selection. Libraries were quality checked by Agilent High Sensitivity DNA Kit using an Agilent 2100 Bianalyzer, and qualified libraries should have a single peak and no junctions. The libraries were quantified using the Quant-iT PicoGreen dsDNAAssay Kit. Select the optimal loading volume and use the single-stranded library as a template for bridge pcr amplification sequencing primer annealing and sequencing while synthesising