title: GSM6840520: H3K27ac_Epi; Gallus gallus; OTHER
library_construction_protocol: For CUT&RUN experiments,dissected neural folds were dissociated using Accumax (Innovative Cell Technologies, AM105) and 500,000 cells were used per CUT&RUN sample. CUT&RUN was performed as previously described 47. The CUTANA™ pAG-MNase (EpiCypher, 15-1016) was used MNase digestion steps. CUT&RUN libraries were prepared using the NEBNext ultra II DNA library prep kit (New England Biolabs, E7645). Quality control of prepared libraries was conducted using an ABI 3730xl DNA analyzer for fragment analysis. Libraries were pooled to equimolar concentrations using the KAPA library quant kit ROX low qPCR mix (Roche, 07960336001) and sequenced with paired-end 37-bp reads on an Illumina NextSeq 500 instrument.