title: Whole genome sequencing of patients with kidney disease from the Nephrotic Syndrome Study Network (NEPTUNE) study.: Sample Sample1519
design: Patient blood samples were collected using the PAXgene Blood DNA System (Qiagen). DNA was then isolated using the PreAnalytix DNA isolation kit designed for PaxGene according the manufactuer's instructions (PreAnalytiX). DNA was digested and then ligated with Illumina sequencing adapters (IDT for Illumnia -TruSeq DNA UD Indexes) and sequenced on an Illumina HiSeq 4000 (Illumnia) using the 2 x 150bp cycles.