title: RNA-Seq of mus musculus
design: 3 L total RNA was used as a template, and NEBNext Multiplex Small RNA         Library Prep Set for Illumina (Catalog NO. E7300, New England Biolabs) was used to build the         library, following the manufacturers instructions with modification. The DNA library was         purified by using 6 % PAGE gel, quantified by using QubitTM3 fluorometer (Invitrogen, Cat.         No. Q33216) with Qubit dsDNA HS Assay Kit (Invitrogen, Cat. No. Q32854), examined by using         Qsep100TM bio-fragment analyzer (Bioptic Inc., Taiwan, China), and finally sequenced on         Novaseq 6000 sequencer (Illumina) with PE150 model.