title: GSM8316107: T18, snucRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Nuclei isolation was performed as previously described (Slyper et al., 2020). Low-retention microcentrifuge tubes (Fisher Scientific, Hampton, NH, USA) were used throughout the procedure to minimize nuclei loss. Briefly, OCT-embedded tissue fragments were quickly thawed on wet ice to remove excess OCT, tissue was next mechanically dissociated by pipetting and homogenized in TST solution, filtered through a 30 um MACS SmartStrainer (Miltenyi Biotec, Germany), and pelleted by centrifugation for four minutes at 500g at 4C. The final nuclei pellet was resuspended in 150 ul of 10x Genomics Diluted Nuclei Buffer, and trypan blue-stained nuclei were counted by eye using INCYTO C-Chip Neubauer Improved Disposable Hemacytometers (VWR International Ltd., Radnor, PA, USA). Chromium Next GEM Single Cell 3ʹ library according to manufactures instructions