title: GSM7674353: Bone marrow-derived macrophages, FGK45-treated, INPUT; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde for 10 min and neutralized with 0.125 M glycine for 5 min at room temperature, and cells were washed with cold PBS and lysed in cold lysis buffer on ice. The cell lysate was sonicated and then incubated with anti-NF-κB2 antibody pre-coated on Dynabeads protein G (Life Technologies) at 4°C overnight. Immunoprecipitated products were collected and washed with RIPA buffer twice and LiCl washing buffer three times. Then, the immunoprecipitated products were digested with Proteinase K, and the formaldehyde cross-links were reversed. The purified DNA fragments were sent for high-throughput sequencing. The purified DNA fragments were sent for high-throughput sequencing. Reads were mapped to the mouse genome (hg19) using “bowtie” software. Results from the ChIP-seq were visualized in Integrative Genomics Viewer.