title: MiSeq of hot spring biofilm and sediment
design: DNA was extracted using Dneasy PowerSoil Kit. The quality of extracted gDNA was checked by electrophoresis. 16S rRNA genes (V4-5) were amplified using primers 515F and 926R.  PCR products were checked by 2% agarose gel electrophoresis and purified with Qiagen Gel Extraction Kit. Barcoded libraries were generated with NEBNext UltraTM DNA Library Prep Kit. Qubit 2.0 fluorometric quantification and Agilent BioAnalyzer were used to check libraries quantity and quality, respectively. Multiplexed 2250 bp paired-end reads were then sequenced on MiSeq Illumina sequencing platform.