title: Synthetic metagenomic sequencing-Validation PsasJ
design: DNA amplicon libraries were generated using a limited cycle PCR: initial denaturation at 95C for 3 min, followed by 25 cycles of annealing (95C 30 seconds, 55C 30 seconds, 72C 30seconds) extension at 72C 5 minutes, using a KAPA HiFi HotStart ReadyMix (KK2602) (Sigma-Aldrich, St Louis, MO, USA). Then llumina sequencing adaptors and dual-index barcodes (Nextera XT index kit v2, FC-131-2001) were added to the amplicons. Libraries were normalized and pooled prior to sequencing.