title: GSM7426040: WT,Ly49HposNK,d4,Spleen,4,11821; Mus musculus; RNA-Seq
library_construction_protocol: Wildtype or TNFR2-/- Ly49H+ NK cells (Ly49H+ CD49b+ TCRb- CD3- CD19- F480-) were sorted from the spleens of mixed bone marrow chimera mice 1, 2, or 4 days p.i with MCMV. In each independent experiment, RNA was isolated from sorted cells using the Arcturus PicoPure RNA Isolation Kit (ThermoFisher) in accordance with the manufacturer instructions. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 0.7-2 ng total RNA with RNA integrity numbers ranging from 9.2 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 9-10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a NovaSeq 6000 in a PE50.