title: environmental sample, 16S rDNA
design: the sequences of 341F (5'-CCT ACG GGN GGC WGC AG-3') and 785R (5'-GAC TAC HVG GGT ATC TAA TCC-3') primers were used to amplify the selected 16S rDNA region and to prepare the library (Thijs et al., 2017). PCR was performed with Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs, Ipswich, USA). Sequencing was carried out on the MiSeq apparatus, in the paired-end (PE), 2x300 nt technology, using the Illumina v3 kit (Illumina, San Diego, USA).