title: PacBio HiFi sequencing data from Dipodomys merriamy: liver sample from adult male
design: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (PacBio, Cat. #100-938-900) according to the manufacturer's instructions.  HMW gDNA was sheared to a target DNA size distribution between 15 kb  18 kb.  The sheared gDNA was concentrated using 0.45X of AMPure PB beads (Pacific Biosciences - PacBio, Menlo Park, CA; Cat. #100-265-900) for the removal of single-strand overhangs at 37C for 15 minutes, followed by further enzymatic steps of DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 10 minutes and 65C for 30 minutes, and ligation of overhang adapter v3 at 20C for 60 .  The SMRTbell library was purified and concentrated with 1X Ampure PB beads (PacBio, Cat. #100-265-900) for nuclease treatment at 37C for 30 minutes and size selection using the BluePippin/PippinHT system  (Sage Science, Beverly, MA; Cat #BLF7510/HPE7510) to collect fragments greater than 7-9 kb.  The 15  20 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using eleven 8M SMRT cells, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.