title: RNA-seq of Prionotus carolinus: uninjected leg 1 after separation
design: RNA extraction was performed using an RNeasy micro kit (Qiagen). RNA input was normalized across samples and RNA libraries were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara), followed by the Nextera XT DNA library preparation kit (Illumina) per manufacturer instructions.