title: GSM8241803: RNA-seq, polyA RNA, 2205 in vivo, sgRT, 6-day AG120, Replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were harvested at serial time points (Vehcle, AG120-Day3 and AG120-Day 6). sgCtrl, sgCgas and sgRT CKIR132C ICC cells were isolated by magnetic bead–mediated depletion of stromal populations from tumors treated with vehicle or AG120 for 3 or 6 days. Single-cell suspensions from cells cultured in a monolayer were homogenized in 300 to 600 μL of TRIzol. RNA was extracted from homogenized samples using the Direct-zol RNA Miniprep Plus Kit in accordance with the manufacturer's suggested protocol. NEBNext Ultra™ II RNA Library Prep Kit for Illumina