title: GSM7673367: L19M neonatal epidermis; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the neonatal epidermal tissue using TRIzol reagent (Thermo Fisher Scientific). After quality assessment and RNA quantification, libraries were prepared from total RNA using a NeBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA was isolated using a poly (A) RNA selection kit (Lexogen, Wien, Austria) and subjected to cDNA synthesis and shearing according to the manufacturer's instructions.