title: Illumina NovaSeq 6000 sequencing; A high-resolution single-cell multiomic atlas of the human fetal lung - Visium
design: A high-resolution single-cell multiomic atlas of the human fetal lung - Visium
library_construction_protocol: Twelve-twenty post conception week foetal lung samples from the HDBR, up to 0.5cm3Â in size, were embedded in OCT and flash frozen in dry-ice cooled isopentane. Twelve micron cryosections were cut onto Visium slides, haematoxylin and eosin stained and imaged at 20X magnification on a Hamamatsu Nanozoomer 2.0 HT Brightfield. Nucleic acid was extracted according to the standard protocol of the Spatial 3' v1 protocol Tissue sections were loaded onto Visium chips according to the standard protocol of the Spatial 3' v1 protocol. Dual-indexed libraries were prepared, pooled at 2.25nM and sequenced.