title: GSM8241861: Het_Neutral_K27ac_R2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were incubated for 10min at room temperature with 1% formaldehyde. Cross-linking was terminated by addition of glycine to a final concentration of 125mM. ChIP was performed using iDeal ChIP-seq Kit (Diagenode) according to the manufacturer's instructions. Antibodies against H3K4me3 (ab8580, Abcam), H3K4me1 (ab8895, Abcam), H3K27ac (ab4729, Abcam), H3K27me3 (ab6002, Abcam) and H3K9me3 (ab8898, Abcam) were used. 10ng of chromatin-immunoprecipitated DNA was end-repaired and dA-tailed. Subsequently, adaptor was ligated to the dA-tailed DNA. DNA was cleaned up using AMpure XP beads after each step. Adaptor-ligated DNA was size selected with AMpure XP beads using different DNA to beads ratio to an average fragment size of 200bp. At last step, size-selected and adaptor-ligated DNA was enriched by PCR with different index primers and cleaned up using AMpure XP beads. Purified library DNA was quantified with Qubit 2.0 and fragment size was assessed using Bioanalyzer (Agilent high sensitivity chip).