title: GSM8190557: NC(no antibody)_2h_RAW264.7; Mus musculus; OTHER
library_construction_protocol: 50000 cells were harvested through HyperactiveUniversalCUT&TagAssayKitfor Illumina (TD903, Vazyme). Cut&Tag libraries were prepared by  the HyperactiveUniversalCUT&TagAssayKitfor Illumina (TD903, Vazyme), complemented with TruePrep Index Kit V2 for Illumina. 40 ng of DNA was  using as starting material. Libraries were amplified using 10 cycles on thermocycler. Post amplification libraries were sized selected at 250-450bp using VAHTS DNA Clean Beads. Libraries were valided using Agilent2100 Bioanalyzer.