title: GSM8190456: FAP1_Mutant_MNase; Saccharomyces cerevisiae; MNase-Seq
library_construction_protocol: Cultures were cross-linked with 1% formaldehyde at room temperature (RT) for 30 min. and quenched with 0.125 M glycine at RT for 5 min. to neutralize the formaldehyde. Cells were centrifuged, washed withwater, and treated with Buffer Z (0.56M sorbitol, 50mM Tris) with 0.5 mL of 10mg/ml zymolyase at 25°C for 30 min. Extracts were centrifuged at 1500 rpm for 6 min. at 4°