title: GSM6736057: mP4_CTRL_Week1_Res3; Mus musculus; RNA-Seq
library_construction_protocol: Organoid cultures (3-4 50 µl domes/condition) were harvested with cold Cell Recovery Solution (Corning, 354253) and incubated on ice for 30 minutes to dissolve Matrigel and release the cells. RNA from the organoid pellets was isolated and purified using RNeasy Mini Kit (QIAGEN, 74106) according to the manufacturer's protocol, including DNase digestion step. RNA concentration was measured using NanoDrop 2000 spectrophotometer and samples were stored at –80°C. RNA-seq libraries were prepared following the standard protocol of DNBSEQ