title: GSM7504785: T cells, Trib1_KO; Mus musculus; OTHER
library_construction_protocol: Activated (CD44+) T cells (TCRb+) were FACS sorted from spleens of control or Trib1 cKO mice at day 15 post-clone 13 infection. Two biological replicates were pooled for each genotype. Cells were washed twice with RPMI, counted and single-cell TCR-seq was performed according to 10X Genomics protocols, targeting 5000 cells for loading. Library construction was performed using the Chromium Single Cell 5' Library and Gel Bead Kit according to the manufacturer's protocol. The final pooled library (control and Trib1 cKO samples) was sequenced on a NextSeq 500 using paired-end sequencing on one 300 cycle high output FlowCell (Illumina).