title: GSM7140174: WTCHG_853846_236_CT_MenACWY_+ve; Homo sapiens; miRNA-Seq
library_construction_protocol: Peripheral blood (up to 2.5ml) was collected into a PAXgeneTM RNA tube at vaccination (D0), SARS-CoV-2 diagnosis (CT) and 7 days post diagnosis (CT+7). Total RNA was extracted using the PAXgene blood RNA kit (PreAnalytiX, Switzerland) on the QIAsymphony SP (Qiagen) with the PAX_RNA V5 protocol. Eluted RNA was quantified using the Agilent RNA ScreenTape assay on the 4200 TapeStation System (Agilent). Small RNA libraries were prepared with the NEBNext® Small RNA Library Prep Set for Illumina (catalog #E7330) and run on a single flow cell target of 300 million reads) on the NexTSeq platform using the 75SR configuration with the aim of achieving 5.2 million reads per sample.