title: GSM7504078: esg-ATAC; Drosophila melanogaster; ATAC-seq
library_construction_protocol: For each sample, around 40,000 PI- GFP+ (DAPI-; GFPweek ; mCherry+) cells were FACS sorted into ice-cold PBS and collected by centrifugation. Cell lysis, DNA Tagmentation by Tn5 were carried out using the ATAC-Seq Kit (Active motif, Catalog No. 53150), following the manufacturer's instructions. Tagmented DNA were amplified with N50x, N70x index to construct the sequencing library, also using the ATAC-Seq Kit (Active motif, Catalog No. 53150), following the manufacturer's instructions. Amplified sequencing library is then purfied using 1.2x magnetic beads, and sequenced on a Hiseq platform.