title: RNA-seq of Akkermansia muciniphila in mucin medium
design: Akkermansia muciniphila BAA-835 was grown in mucin medium, using mucin as the sole source of carbon and nitrogen, to an OD600 of 0.4. Cells were treated with Qiagen RNAprotect Bacteria Reagent and RNA was isolated using a Qiagen RNeasy Mini Kit. RNA quality was checked with an Agilent Bioanalyzer instrument and ribosomal RNA was removed using Ribominus Bacteria (Thermo). Sequencing libraries were prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina kit (NEB) and sequencing was performed at the Duke Sequencing Core Facility.