title: GSM6736361: WT 2; Saccharomyces cerevisiae; MNase-Seq
library_construction_protocol: Preparation of mono-nucleosomal DNA was performed as described (Lantermann et al. 2009) followed by high throughput sequencing on Illumina HiSeq 4000 platform. Briefly, cells were grown under the specified conditions and then fixed with 1% formaldehide. Spheroblasts were obtained and the chromatin digested with 30u/ml of Mnase (ThermoFisher) at 37°C for 20 minutes. The samples were then treated with Proteinase K and RNase A to isolate the undigested, mono-nucleosomal DNA. The DNA was then size selected and purified in a 1.5% agarose gel. Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer's protocol.