title: GSM6641965: CTRL1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in Trizol (Invitrogen) and total RNA was extracted. Library construction and sequencing was performed by BGI Genomics, BGI-Wuhan. RNA were treated with DNase I and purified with magnetic beads with Oligo (dT). RNA were mixed with the fragmentation buffer and were fragmented into short fragments. Then cDNA was synthesized using the mRNA fragments as templates. Short fragments were purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments were connected with adapters. Suitable fragments were selected for the PCR amplification as templates. Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used for quantification and qualification of the sample library. 150bp paired sequencing was performed using Illumina HiSeqTM4000.