title: GSM6401991: S-Evs, MAC2A [MAC2A]; Homo sapiens; miRNA-Seq
library_construction_protocol: Circulating S-EVs RNA was obtained from 0.5 mL plasma by using the exoRNeasy Midi kit (Qiagen, Hilden, Germany), following the manufacturer's instructions. Total RNA from ALCL primary tumors was obtained using Trizol reagent (ThermoFisher Scientific) according to the manufacturer's protocol. Cultured cells were processed for RNA isolation using the total RNA Purification Kit (Norgen Biotek Corp., Thorold, Canada) as per the manufacturer's protocol. Small RNA-seq libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) and sequenced single-end on an Illumina HiSeq 4000 platform (Illumina, San Diego, CA).