title: GSM7025135: iPSC-control, clone 2, replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the NuceloSpin® RNA kit (Macherey-Nagel) according to the manufacturer's instructions. Concentration and quality of extracted RNA were measured using the Qubit 4 Fluorometer (Thermo Fisher Scientific, MA, USA) and the 2100 Bioanalyzer Instrument (Agilent Technologies, CA, USA) using an RNA 6000 Nano Kit. Libraries from mRNA were prepared using 800 ng of RNA starting material and the TruSeq Stranded mRNA Library Prep Kit (Illumina, CA, USA) according to the manufacturer's protocol. This protocol captures poly-adenylated RNA by transcription by oligo-dT primer, after which the RNA is fragmented. The sample is back transcribed to generate the cDNA, both in the first and second strands. The 3 'ends are adenylated, the adapters and barcodes are ligated, and finally, it is enriched by PCR. Adapters and samples codes (index-barcodes) are added to the libraries to be able to be sequenced simultaneously. mRNA libraries were sequenced on the NextSeq 500 system (Illumina, CA, USA) using the highest output mode and paired-end 75 bp read lengths with a depth of 25 million reads for each sample.