title: GSM7025132: Multiplexed Day7 Reprogramming scRNASeq; Homo sapiens; RNA-Seq
library_construction_protocol: Normal, Lamin A/C knockdown, and HGPS fibroblasts were lifted 7 days after reprogramming and prepared for multiplexed single-cell RNA sequencing. Normal, Lamin A/C knockdown, and HGPS fibroblasts were each labeled with 3' CellPlex cell multiplexing oligos (CMO; 10X Genomics) and pooled together before loading onto the 10X Genomics chromium single cell controller for single-cell RNA sequencing. The CMOs used for each sample were: Normal-CMO309, HGPS-CMO310, and Lmna KD-CMO311. 30,000 total cells were targeted with 50,000 reads per cell. Libraries were sequenced on a NovaSeq 6000 (Illumina) and aligned to the refdata-gex-GRCh38-2020-A reference transcriptome using CellRanger v7.1.0 (10X Genomics).