title: ITS METAGENOME KERNEL
design: DNA was purified with Wizard Food DNA kit (Promega), and quantified with a Nanodrop spectrophotometer (Thermo Scientific). The variable ITS2 region of ITS rRNA was amplified using the universal primers ITS3F ITS4R, and barcoded with Nextera indexes, according to manufacturer instructions (Illumina). The amplicons were them quantified with Qubit DNA HS kit (Thermo), and sequenced with NextSeq P1-600 (Ilumina), in pairend 2x300b