title: GSM7984629: E2-1-RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Mini Kit (Qiagen). The quantity and quality of the RNA samples were assessed by Tapestation. Polyadenylated mRNAs were selected using Dynabeads Oligo(dT)25 (Invitrogen #61005) and libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7760) following manufacturer's instruction. Libraries were size selected using AMPure XP Beads (Beckman Coulter). Libraries were quantified Qubit Flex and Tapestation and then sequenced with 75 bp single-end reads on Illumina NextSeq high-output.