title: GSM7310739: AB5272; Mus musculus; RNA-Seq
library_construction_protocol: Isolation of tumor cells and tumor-infiltrating immune cells was performed from tumor-bearing brain hemispheres. Briefly mice were perfused with PBS and the tumor-bearing brain hemisphere was collected in Roswell Park Memorial Institute (RPMI) medium containing 0.4 mg/ml Collagenase IV and 0.1 mg/ml DNase I (both Sigma-Aldrich). The tissue was then incubated for 40 min at 37°C and further homogenize using a 18G needle. Myelin was removed using a 30% Percoll centrifugation step (Sigma-Aldrich). 3' end mRNA libraries were prepared for sequencing using MARS-seq (Jaitin et al, Science 2014) single-cell RNA-sequencing for gene expression quantitation