title: GSM8145477: HEK293T, STM2457, biol rep1, MERIP-seq, Input; Homo sapiens; OTHER
library_construction_protocol: RNA was harvested using RNeasy mini plus kit (Qiagen). 4 ug of total RNA was used for the construction of sequencing libraries. rRNA was depleted by TransNGS rRNA Depletion Kit. The MeRIP-seq libraries were prepared by RNA fragmentation, 3' adapter ligation, sample pooling, m6A IP, 5' adapter ligation, cDNA synthesis and amplification.