title: GSM6433214: Tumor-infiltrating lymphocytes, control [Control_S1_L001]; Mus musculus; RNA-Seq
library_construction_protocol: Fresh tissue of vehicle or rmIL-27 treated mouse  tumors was collected in cold RPMI media, cut into small pieces with surgical scissors, digested with Gentle MACS Dissociator (MiltenyiBiotec) for 45 min at 37°C on a shaker, and consequently meshed through a Falcon 70-um cell strainer. After PBS washing, red cells were lysed with an ammonium chloride solution. Tumor infiltrating lymphocytes were sorted using mouse CD45 (TIL) MicroBeads (MiltenyiBiotec)  according to the protocol of MACS® Separation. Single-cell RNA libraries were prepared using the Chromium Single Cell 5'v2 Reagent (10x Genomics), and Chromium Single Cell V(D)J Reagent kits (10x Genomics) was used to prepare single-cell RNA libraries according to the manufacturer s instructions. Each sequencing library was generated with a unique sample index. Sequencing libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). The libraries were sequenced on NovaSeq6000 (Illumina) using 2x150 chemistry.