title: GSM8416683: TYW2 timecourse Lane 3 MULTI; Mus musculus; RNA-Seq
library_construction_protocol: Primary tumors were surgically removed, dissociated, and cryopreserved with CELLBANNKER2. Cryopreserved samples were then thawed, 3-4 tumors from each background/timepoint were pooled, and pooled tumor cells were barcoded with  MULTI-seq lipid-modified oligonucleotides. Barcoded samples from each background/timepoint were then pooled into BSA-containing PBS prior to FACS isolation of CD45+ tumor immune cells. Libraries were prepared according to manufacturers instructions (10x Genomics V3.1) with a separate MULTI-seq library prep workflow performed following cDNA amplification, as described previously (McGinnis, Patterson, et al., Nature Methods, 2019)