title: DNA-seq of Lupinus luteus: young leaf
design: The nucleic acid extractions were performed from young leaves (~60 mg). According to manufacturers instructions, DNA extraction was performed with Quick-DNA HMW MagBead and Quick-DNA Plant/Seed Kits (Zymo Research). The quantification and quality ratios (260/280 & 260/230) of nucleic acid were measured by absorbance in the Synergy HTX multi-plate reader with a Take3 Trio plate (Biotek), and the integrity was assessed through 1%agarose gel electrophoresis  and run at 100V for 1 hour.  After extraction ~500 ng of gDNA was sequenced on the Illumina NovaSeq 6000 150PE plataform