title: GSM6736620: bam mutant rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 120 pairs of ovaries were dissected for each sample and immediately stored at -80°C after dissection. Frozen samples were homogenized in TRIzol Reagent (Invitrogen) using an electrical pestle and further disrupted by passing 15 times through a 26-gauge syringe. Total RNA was isolated using TRIzol Reagent (Invitrogen) following the manufacturer9s protocol.  Poly(A)-selected RNA-sequencing (RNA- seq) libraries were generated using 2.5 μg of purified RNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module and the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina®. Libraries were multiplexed using the NEBNext® Multiplex Oligos for Illumina® and sequenced in single-end, 50-nt-long reads on an Illumina HiSeq 2500. 