title: Genome sequence of antagonistic strain KRS009
design: DNA is first processed into fragments of appropriate size by g-TUBE.Then the fragment undergoes damage repair and end repair, and both ends of the DNA fragment are connected to a hairpin structure to form a dumbbell structure, known as SMRTbell.The annealed smrtbell is mixed with the polymerase at the bottom of ZWM, which will be used for final sequencing.