title: GSM8417504: CC39034045; Homo sapiens; RNA-Seq
library_construction_protocol: TRIzol method. Homogenized samples were thawed on ice followed by the addition of 200µL of chloroform and thorough mixing, then a  5 min incubation at room temperature, followed by centrifugation for 15 min at 40ºC at 12000g.  The clear, aqueous top layer was collected and total RNA was precipitated by adding 100% cold ethanol to the extracted portion until a final concentration of 35% ethanol was achieved. Samples were then incubated for 30 minutes at 200C and centrifuged for 15 minutes at 40C at 16000g.  75% ethanol was used to wash the RNA pellet twice, and the RNA pellet was left to air-dry after which it was resuspended in 20µL of nuclease-free, ultra-pure diethlpyrocarbonate-treated water. Libraries were created using a non-strand specific library of polyA RNA (Illumina TruSeq RNA Sample Prep Kit v2, Illumina, San Diego, CA)