title: GSM6599380: GES1 cells, Input; Homo sapiens; RIP-Seq
library_construction_protocol: Genomic DNA was extracted from the cell lines, treated with RNase A, and sonicated to produce fragments. Fragmented DNA was incubated with an anti-6mA polyclonal antibody (Synaptic Systems) in immunoprecipitation buffer. The mixture was immunoprecipitated by incubation with protein A/G magnetic beads (Thermo Scientific) at 4°C overnight. After washing, the bound DNA was eluted from the beads at 65°C, treated with proteinase K, and purified using the QIAquick PCR Purification Kit (QIAGEN). Input was used as the control. Libraries for input and immunoprecipitated DNA were prepared and sequenced. DNA libraries were prepared for sequencing using standard Illumina protocols