title: GSM6735814: adult cardiomyocytes, 30 min 4sU, rep5; Mus musculus; RNA-Seq
library_construction_protocol: Hearts were excised and digested via retrograde perfusion with Liberase as described in Rudolph et. al. 2019 (https://doi.org/10.1073/pnas.1904385116). After perfusion, the left ventricle was cut, minced and dissociated py pipetting. Cardiomyocyted were sedimented by gravity any the pellett resuspended in TRIzol. 10mM 4sU was added to the buffers from mincing to sedimentation. Dissociation was performed at 42°C instead of 37°C RNA was extracted using TRIzol-Chloroform-Isopropanol according to the manufacturer's instructions. Iodiacetamide Treatment was carried out on extracted RNA in 50% DMSO as described in Herzog et. Al 2017 (https://doi.org/10.1038/nmeth.4435). libraries for RNA-seq were prepared following a CELseq protocol as published in Holler & Junker 2019 (https://doi.org/10.1007/978-1-4939-9009-2_9). Sequencing adapters were switched – Insert is in read 1!