title: GSM7306547: Mock riboseq, 5min, rep1; Lactococcus lactis; OTHER
library_construction_protocol: Cells were pelleted by centrifugation; pellets were frozen and pulverized in Retsch Mixer Mill 400. 25 AU of lysate were supplemented with 5 mM CaCl2 and digested with 1500 U of Micrococcal nuclease (Roche) at 25°C for 1 hour with agitation at 1400 rpm. Following end repair, pre-adenylated linkers bearing unique molecular identifiers (UMIs) and sample barcodes were ligated to footprints. rRNA removal was done with Ribo-Zero Magnetic kit for Gram-positive bacteria (Epicentre) according to the manufacturer's protocol. Single-stranded cDNA was circularized and then amplified by 4 rounds of PCR. Deep sequencing was performed with the Illumina HiSEQ4000. Other:Ribo-seq