title: GSM6736724: LU10BPA_rep2 [Sample_15842]; Mus musculus; ATAC-seq
library_construction_protocol: Fresh frozen lung and brain tissue sections from C57BL/6J mice were collected on a UV-activated PEN slide and further tagmented in situ using an in-house Tn5 enzyme for 1 h at 37 °C. Following, LCM was applied to collect a mini bulk of randomly captured nuclei (PALM microdissection microscope, Zeiss). Two mini-bulk sizes were investigated: 50 single-captured nuclei or 10 larger captured-areas composed each of roughly 20 nuclei, for a total of ~200 nuclei. This was applied both on brain and lung samples, at least in technical duplicates and nuclei were collected in reverse-crosslinking buffer containing Proteinase K and SDS. After reverse crosslinking at 65 °C for 1 hour, tagmented fragments were amplified, purified and sequenced paired-end on a Illumina NextSeq 2000. Barcoded Nextera i7 and i5 primers were used to amplify the tagmented gDNA and preapare it for Illumina sequencing ATAC-seq - Paired End 50 on a NovaSeq6000 (S1 v1.5 chemistry)