title: GSM8190190: sgPtch1-sgKmt2c tumors rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs extracted with RNeasy Plus Mini Kit (74134, QIAGEN) were used for RNA-seq differential expression analysis in some experiments. For RNA sequencing, 450-700 ng of the RNA was used to prepare each library using the SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara, 634874), according to the provided guidelines.