title: GSM8098778: Seed maturation stage MATURE biol rep 1; Thlaspi arvense; RNA-Seq
library_construction_protocol: Total RNA was isolated from 0.1 g of Thlaspi arvense seeds from the five maturation stages analyzed using the CTAB-LiCl extraction method of Gasic et al. (2004). RNA concentration and integrity were measured in a Nanodrop 2000 UV-Vis Spectrophotometer (Thermo Scientific). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. The first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced.