title: GSM6461744: PolyA RNASeq 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA isolation was performed with Qiagen RNeasy kit. Polyadenylated RNA transcripts were isolated using two rounds of poly-A capture on Oligo dT(25) magnetic beads. Isolated polyadenylated RNA was fragmented by heating, treated with DNAse and dephosphorylated using FastAP and T4 PNK. Then, RNA was ligated with a ssRNA adaptor and reverse transcribed into cDNA. The cDNA was ligated with the second adaptor and amplified using standard Illumina library prep. Libraries were gel cut and sequenced on a HiSeq 2500 (90 cycle x 125 cycle).