title: GSM6402152: Naïve Young D2; Mus musculus; RNA-Seq
library_construction_protocol: For the contusion injury, mice were anesthetized with ketamine (100 mg/kg)-xylazine (10 mg/kg) mixture. A 15-mm midline skin incision was made, and the connective tissue and muscles were bluntly dissected to expose T6-T13. Laminectomy was performed at T9 to expose the dorsal spinal cord, and the vertebral column was stabilized with angled clamps attached to the T7 and T12 transverse processes. The contusion injury was delivered using an Infinite Horizon Impactor (IH-0400 Impactor) with a force of 90 dynes. After injury, the muscles and skin were sutured separately. Mice were humanely euthanized with an intraperitoneal injection of a lethal dose of Euthasol® (10% v/v), followed by transcardiac perfusion of PBS with 5 U/mL of Heparin. Vertebral columns were obtained. The ventral bone of the vertebrae was cut laterally and was removed to expose the spinal cord. Nervous tissue was carefully dissected from the bone using fine forceps to release the dorsal root ganglia surrounded by the meninges. The meninges was cut along the ventral side and peeled from the spinal cord. Spinal cord meninges was enzymatically digested in RPMI containing 1 mg/mL collagenase VIII, 0.5 mg/mL DNase I, and 2% FBS for 30 minutes at 37°C with shaking. Samples were passed through a 70 µ