title: GSM6466130: Sample_R21072416-aIgM-LPS-NAC-aIgM-LPS-NAC; Mus musculus; ATAC-seq
library_construction_protocol: Cell sample reference 'ATAC-seq: A Method for Assaying Chromatin Accessibility genome-wide' lysis of cells to obtain nuclei Transposition reaction: 40000 cell nuclei were processed with Tn5 enzyme (Illumina) and the transposition reaction was processed. The DNA fragment after transposition was retrieved with MinElute PCR Purification Kit(Qiagen).  Database construction: 1X NEBNext High-Fidelity PCR Master Mix (New England Biolabs, MA) was used for PCR amplification. The amplification product was recovered by MinElute PCR Purification Kit (Qiagen).  Sequencing was performed with Illumina Novaseq 6000.