title: GSM7212167: RNAseq_SCd_2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA extraction, cells were collected on ice, washed in PBS and centrifuged at 1000rpm for 5 minutes at 4oC. Pellets were lysed with TRIzolTM Reagent (Thermo Fisher Scientific). RNA was isolated by chloroform extraction followed by centrifugation, isopropanol precipitation, washing in 75% ethanol and resuspension in nuclease-free water. Libraries were generated with the TrueSeq Stranded mRNA kit (Illumina).