title: GSM7847836: 19_WT_Wake; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were isolated from frozen hippocampus tissues. Samples were homogenized in lysis buffer (10 mM Tris HCL pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 40 U/mL of RNAse inhibitor ) followed by spin down at 500xf for 5min at 4°C. The supernatant was removed, and the pellets were resuspended in 5ml wash buffer (PBS, 1% BSA, 40U/mL of RNAse inhibitors) and centrifuged again at 500g for 5 mins at 4°C. The supernatant was removed and the pellet was gently resuspended in 250 μL of wash buffer. Nuclei were filtered through a 30 μm filter and counted. GEM generation and libarary construction were done according to manufacturer instructions of 10x Genomics Chromium Single Cell 3' Reagent Kit (v3.1 Chemistry)