title: GSM6463425: Col0_XVE-ROS1_Estradiol_Nanopore-seq; Arabidopsis thaliana; OTHER
library_construction_protocol: On day 20 and 48 hrs after the second spray treatment, aerial tissue from c. 100 seedlings (c. 500 mg FW) were harvested and extracted for DNA using the NucleoBond® HMW DNA kit (Macherey Nagel, 740160.20) according to the manufacturer's instructions Library preparation was conducted using the Oxford Nanopore Technologies (ONT) Rapid Barcoding kit (SQK-RBK004). The prepared libary was loaded into the Flow Cell (ONT, FLO-MIN106) of a MinION sequencer. The Flow Cell was washed using Flow Cell Wash Kit (ONT, EXP-WSH004) after 2 days of sequencing, and then ran to exhaustation.