title: GSM8241358: Non-lipid-laden monocyte-derived macrophages, mn:547; Mus musculus; ATAC-seq
library_construction_protocol: Nuclei of the FACS sorted cells were isolated, permeabilized and tagmented by using in-house-generated Tn5 transposase. The resulting DNA fragments underwent a first 9-cycle PCR amplifications using KAPA HiFi HotStart ReadyMix (7958935001, Roche), and we selected the fragments between 150 and 700 bp in size using with SPRI beads (A63881, Backman Coulter). ATAC-seq libraries were prepared using an optimized protocol.103  The resulting DNA fragments underwent a first 9-cycle PCR amplifications using KAPA HiFi HotStart ReadyMix (7958935001, Roche), and we selected the fragments between 150 and 700 bp in size using with SPRI beads (A63881, Backman Coulter). The size-selected fragments were amplified for a second 9-cycle PCR reactions, and the resulted libraries were purified using SPRI beads.