title: GSM6640414: Escherichia coli SAPYR treated, not irradiated sample: replicate R2; Escherichia coli; RNA-Seq
library_construction_protocol: RNA extraction and sequencing were performed by the NGS Competence Center Tübingen (NCCT), the sequencing partner of the Quantitative Biology Center (QBiC), University of Tübingen, Germany. RNA of all samples was isolated with the RNeasy® Mini-Kit (Qiagen, Hilden, Germany). RNA extraction was followed by DNase I treatment in solution using the DNase I recombinant Kit (Roche, Basel, Switzerland) and purification and concentration of RNA using the RNA Clean & Concentrator-5 Kit (Zymo Research, Freiburg, Germany). Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit (Illumina, San Diego, CA, USA) with 100 ng total RNA. Sequencing was performed on the Illumina NextSeq 500 using a High Output Kit (version 2.5; 75 cycles) with 1% PhiX spike-in (Illumina, San Diego, CA, USA).