title: GSM7502641: SB_sk; Mus musculus; RNA-Seq
library_construction_protocol: Skull marrow samples from Sham and i.c. SB28 injected mouse were harvested by immediate decapitation posterior to the occipital bone after perfusion with ice-cold HBSS. The drilling site for the intracranial surgery was discarded to avoid mechanical disruption or contamination related to tumor induction.  Single cell suspension was prepared using enzymatic digestion (50% TrypLE (ThermoFisher), 50% Collagenase Type VII-S (Sigma-Aldrich)). Samples were incubated for 10 minutes at 37 degree C, pipetted gently using wide tips, and filtered onto 70 micron cell strainer. Cold Hibernate-E medium (ThermoFisher) with 4% BSA was used for sample collection, washing and resuspension. Cells were sorted using FACS Aria-II to clear debris and dead cells. Sorted cells with viability >70% were kept on ice until processed for library within 1-3 hours. scRNAseq libraries were constructed by the Albert Einstein Genomics Core using the 3' kit protocols of 10X Genomics processed on a Chromium Chip B microfluidic apparatus (10X Genomics).