title: GSM6599157: Liver ORSAM17824-2; Mus musculus; OTHER
library_construction_protocol: Ligated samples underwent crosslink reversal, DNA extraction with proteinase K, and biotin removal from unligated fragments. Promotor capture libraries were constructed via the following method. Crosslinked cells were lysed, digested with DpnII, ends filled in using biotin-14-dATP followed by overnight proximity ligation.  Ligated samples underwent crosslink reversal, DNA extraction with proteinase K, and biotin removal from unligated fragments.  DNA was fragmented to 500-600 bp, column purified, and biotinylated fragments pulled down. Biotinylated fragments were converted into Illumina compatible libraries using the SureSelect XT HS2 DNA System and a custom SureSelect probe set (design ID S3346176) (Agilent Technologies) according to the manufacture's protocol with the following exception:  pre-capture PCR was performed as four 50 ul reactions that were recombined during clean-up. The quality and concentration of the libraries were assessed using the High Sensitivity D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively, according to the manufacturers' instructions. Libraries were sequenced 150 bp paired end on an Illumina NovaSeq 6000.