title: GSM7785528: K562, Y391K-LSD1-GFP, edited1-1; Homo sapiens; RNA-Seq
library_construction_protocol: The harvested cell pellets were flash-frozen and sent to MedGenome (Foster City, CA) for RNA extraction, sequencing, and analysis. Extractions were carried out using a Maxwell RSC simplyRNA Cells Kit (Promega, cat 1390).  To verify RNA quality, RNA qualitative and quantitative assessment was done using Qubit 3.0 (life technology, cat Q33216) and TapeStation 4200 (Agilent) confirming that the RNA Integrity Number equivalent (RINe) score exceeded 8.9 for all samples. The Poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads and then mRNA was converted to cDNA, library preparation using TruSeq Stranded mRNA Library Prep Kit (cat 20020595) according to the manufacturer's protocol. Libraries were sequenced for PE150 cycles to a depth of 40 million paired reads using Illumina NovaSeq 6000.