title: Whole genome long read (ONT Nanopore SMS) sequencing of DNA from single adult female mosquito of Rockefeller (ROCK) pyrethroid susceptible strain
design: HMW DNA extracted from single adult female mosquito by P:C:I phase separation. DNA sheared by Covaris to target 20,000kb. Circulomics size selection removed fragments <7kb. Barcoded DNA library prepared using ONT kit. Library sequenced alongsize second library of same strain on one flow-cell of ONT PromethION.