title: GSM7722486: STHdhQ111/Q111, IFNg/LPS, Rep 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the Direct-ZolTM RNA miniprep Plus kit according to the manufacturer's instruction (Cat. #R2071, ZYMO RESEATCH). To reduce DNA contaminations, a 15-min on-column DNase I treatment was included. Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification. After purification, poly(A) RNA was fragmented using divalent cation buffer in elevated temperature. Quality control analysis and quantification of the sequencing library were performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip. Paired-ended sequencing was performed on Illumina's NovaSeq 6000 sequencing system.