title: GSM8189628: EhracJ gene silenced strain replicate 2; Entamoeba histolytica; RNA-Seq
library_construction_protocol: Logarithmic growth phase of E. histolytica trophozoites were harvested then total RNA was extracted using Trizol reagent. RNA-seq libraries were generated using a directional library preparation protocol. The process included polyA-plus RNA purification, RNA fragmentation, random hexamer primed cDNA synthesis with dUTP, end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, PCR amplification, and purification. The libraries were then subjected to quality control checks with Qubit, real-time PCR, and bioanalyzer before sequencing on Illumina platforms. Finally, the libraries were pooled and sequenced on Illumina Novaseq 6000.