title: NGS of DENV2 isolate: NI/8371
design: Viral genetic material was extracted from cell culture supernatant using the AgenCourt RNAdvance Cell V2 kit. First-strand cDNA synthesis was performed with random hexamers and the Superscript III First-Strand Synthesis System. This was followed by second-strand cDNA synthesis using Klenow exo-enzyme. Library preparation was performed using sparQ DNA Frag & Library Prep Kit. Samples were indexed with molecular ID tags using sparQ adapters.