title: GSM7673621: KO-RZ; Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA was extracted by TRIzol Reagent (Invitrogen), followed by ribosomal and polyA depletion. RNA was processed by RiboZero purification and oligo-dT depletion. RNA Barcoded Libraries were prepared according to Illumina's instructions (2013) accompanying the TruSeQ RNA Sample prep kit v2 (Part# RS‐122‐2001). Libraries were quantitated using the Agilent Bio‐analyzer (model 2100) and pooled in equimolar amounts. Electrophoresis System (Bio-Rad).