title: WGS of Sargassum sp. metagenome
design: DNA library preparation and sequencing were performed by AZENTA, Inc. in South Plainfield, NJ, USA. The NEB NextUltra DNA Library Preparation kit was utilized according to the manufacturer's instructions (Illumina, San Diego, CA, USA). The genomic DNA underwent fragmentation using acoustic shearing via a Covaris S220. The fragmented DNA was then subjected to end-repair, adenylation, and subsequent adapter ligation following the adenylation of 3 ends. Adapter-ligated DNA underwent indexing and enrichment through limited cycle PCR. The resulting libraries were verified using TapeStation (Agilent Technologies, Palo Alto, CA, USA) and quantified using Qubit 2.0 Fluorometer and real-time PCR (Applied Biosystems, Carlsbad, CA, USA). Sequencing was performed on an Illumina HiSeq 4000 (Illumina, San Diego, CA, USA) in a 2x150 paired-end configuration. The base calling was performed using the HiSeq 4000 or equivalent Control Software, and the .bcl files were converted to fastq and demultiplexed using bcl2fastq v. 2.17.