title: GSM8242132: RRBS H3WT, rep1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted using Invitrogen Purelink Genomic DNA Mini kit. Genomic DNA was quantified using a Qubit 3.0 Fluorometer. RRBS was performed on 100 ng of each sample using the NuGen Ovation RRBS Methyl-seq System following the manufacturer's recommendations. Bisulfite conversion of DNA was conducted using the Qiagen EpiTect Fast Bisulfite Conversion kit. Libraries were purified with Agencourt RNAclean XP beads and quality-assessed using an Agilent 4150 Tape-Station D1000 ScreenTape. Libraries were then sequenced on the Illumina NovaSeq6000, generating 100-bp single-end reads.