title: Apo53_M4
design: Aposymbiotic Riptortus pedestris insects were harvested in the fifth instar at day 3. Insects were dissected and the M4 midgut region were harvested in RNAiso Plus (Takara Bio). RNA was extracted with the RNeasy mini kit (Qiagen). mRNA purification and fragmentation and the preparation of cDNA libraries were conducted using TruSeq RNA Library Prep Kit v2 kit (Illumina). The cDNA libraries were sequenced by Illumina HiSeq-2000.