title: WGS of Klebsiella pneumoniae
design: Bacterial DNA was extracted and purified using GeneJET Genomic DNA Purification Kit according to the manufacturers instructions. The extracted DNA was quantified using the Promega QuantiFluor dsDNA System, normalized to 5 ng/L using PCR grade water, and run on a Promega GloMax Discover Microplate Reader. Whole genome sequencing  was performed on an Illumina Nextseq500 instrument using a High Output Flowcell NextSeq 500/550 High Output Kit v2.5. The libraries were quantified using the Glomax and QuantiFluor dsDNA kit. Libraries were pooled and the final pool was double-SPRI size selected between 0.5 and 0.7X bead volume using the beads supplied in the Illumina DNA Prep kit. The final pool was quantified on a Qubit 3.0 instrument and run on a D5000 ScreenTape using the Agilent Tapestation 4200 to calculate the final library pool molarity. The generated BCL files were converted to FASTQ format using bcl2fastq v2.20.0.422 software and used for downstream analysis.