title: WGS of homo sapiens: female vulvar carcinoma
design: The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed, and further ligated with Illumina adapters. The fragments with adapters were size-selected, PCR amplified, and purified. The library was checked with Qubit and real-time PCR for quantification and a bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to the effective library concentration and the data amount required. The original fluorescence image files obtained from the Illumina platform were transformed into short reads (raw data) by base-calling and these short reads were recorded in the FASTQ format, which contains sequences and the corresponding sequencing quality information.