title: GSM7785062: primary CD4sp; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from iPSC-Tregs, iPSC-CD4sp, primary Tregs, induced Tregs and primary Tconv was extracted using an RNA extraction kit (Qiagen) following differentiation (iPSC-Tregs and induced Tregs) or isolation and culture (primary Tregs and Tconv).  Primary cells were isolated from leukapheresis. Library was performed according to the manufacturer's instructions (10x Genomics). Single cell RNA-sequencing libraries were sequenced on a NextSeq 500.