title: GSM8420529: HeLa-WT_RNaseH_input; Homo sapiens; OTHER
library_construction_protocol: Cells were harvested and genomic DNA was isolated. Extracted genomic DNA was digested overnight at 37°C using a restriction enzyme cocktail (BsrGI, EcoRI, HindIII, SspI and XbaI), followed by treatment with mock or RNase H. For each DRIP, 8 µg of digested DNAs were subject to immunoprecipitation with 10 µg of S9.6 antibody and eluted. The eluates were sonicated using the bioruptor (Diagenode) and used for library preparation. Libraries were constructed using the NEBNext Ultra II kit following manufacturer's instructions.