title: GSM7673447: BAP-18; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from HBEC (n=4) using RNeasy Mini Kits (Qiagen) and was quantified on a Synergy HTX plate reader equipped with a Take3 module (BioTek).  RNA quality was evaluated based on 260/280 ratio (acceptable range 2.0-2.1) and by examining 18S and 28S peaks (Bioanalyzer 2100, Agilent, Santa Clara, CA). Acceptable RNA quality was based on RIN ≥ 8.5. mRNA was poly-A selected, and libraries were prepared as previously described (Dasgupta et al., 2022.