title: Internal Transcribed Spacer (ITS) region sequences of fungi collected from the surface of ETICS materials of building facades
design: For fungal community analysis, amplicons targeting the fungal ITS regions were prepared from the pooled DNA samples by PCR using adequate hybrid primers for ITS1f (5- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTTGGTCATTTAGAGGAAGTAA-3) and ITS2 (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTGCGTTCTTCATCGATGC-3) flanked by Illumina universal primer sequencing for barcode insertion.