title: GSM7094372: GVHD mouse dosed with anti-CX3CL1 mAb (0.5 mg/head) [ID_1541]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from the mouse skin using RNeasy spin columns (Qiagen) and digested with DNase I (Qiagen) to remove chromosomal DNA. The preparation of the mRNA-Seq library was carried out using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) in accordance with the manufacturer's instructions optimized to Illumina Multiplexed Sequencing (https://targetepigenomics.org/docs/library_construction_protocol_perlab/Biswal_RNAseq_TruSeq.pdf).