title: GSM7211339: H1299-cont; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol was added to the prepared mammalian cells, reacted for 5 minutes, and then chloroform was added and reacted for 3 minutes. After centrifugation at 4℃ and 1,3000 rpm for 15 minutes, the supernatant was transferred to a new tube. After adding isopropanol and reacting for 10 minutes, centrifugation was performed at 4℃ and 1,3000 rpm for 10 minutes. After discarding the supernatant, add 80% ethanol, centrifuge at 4℃ and 1,3000 rpm for 5 minutes, and discard the supernatant. After air drying for 5 minutes, add sterilized tertiary distilled water and store it. RNA libraries were prepared for sequencing using standard Illumina protocols