title: GSM8317753: ep_OsmrWT, colitis, day 21, E1_2_4; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized via cervical dislocation, and their colons were harvested into ice-cold PBS. After longitudinal opening and cleaning, colons were sectioned into 2 mm pieces and subjected to sequential EDTA washes in RPMI with 10% FCS at 37°C to detach epithelial cells. For the sequencing of VillincreERT2 Osmrfl/fl and VillincreERT2 Osmrfl/+ epithelial cells, EpCAM+ live epithelial cells were FACS-sorted and lysed in the RLT lysis buffer and RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's protocol. RNA integrity was assessed as per the manufacturer's guidelines. The following procedures were performed at Novogene, where sequencing was carried out on a HiSeq X sequencer to generate 150 bp paired-end reads.