title: GSM7135929: Kasumi1, H3K27me3, shHIF2a rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using the Diagenode Bioruptor 300. Library were sequenced using Illumina High throughput Sequencing technology. Samples were loaded on NovaSeq 6000 and sequenced in paried-end mode, generating 100 nt length reads in order to obtain approximately 40M reads. Libraries were validated using the High Sensitivity D5000 ScreenTape (Agilent Technologies).