title: GSM6641836: P23-45_t5_enriched; Thermus thermophilus HB8; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol method, followed by Dnase I treatment. The enriched sames were subjected to an enrichment procedure based on streptavidin beads to enrich for primary RNA transcripts. For the control samples the enrichment steps were omitted. Total RNA was enriched for primary transcripts using the ONT-cappable-seq enrichment protocol. In paralel, the control samples were subjected to similar environmental conditions. The RNA was reverse transcribed, PCR amplified and barcoded according to the Oxford Nanopore Technologies cDNA-PCR protocol (SQK-PCS109 combined with SQK-PBK004). The amplified cDNA samples were pooled together in a final library.