title: GSM6515827: RB1wt/wt 8h; Homo sapiens; RNA-Seq
library_construction_protocol: These samples RNA were isolated with TRIzol reagent (Invitrogen). To select cDNA fragments length of 370~420 bp, the library fragments were purified with AMPure XP system (Beckman  Coulter, Beverly, USA). Then PCR amplification, the PCR product was purified by  AMPure XP beads, and the library was finally obtained