title: GSM8190587: From Severe CRKP pneumonia 1(scBCR seq); Homo sapiens; RNA-Seq
library_construction_protocol: Human blood were dissociated at 37 ℃ with a shaking speed of 50 r.p.m for about 40 min.We repeatedly collected the dissociated cells at interval of 20 min to increase cell yield and viability. Cell suspensions were filtered using a 40 um nylon cell strainer and red blood cells were removed by 1X Red Blood Cell Lysis Solution (Thermo Fisher, Cat. no. 00-4333-57) . Dissociated cells were washed with 1x DPBS containing 2% FBS. Cells were stained with 0.4% Trypan blue (Thermo Fisher, Cat. no. 14190144) to check the viability on Countess® II Automated Cell Counter (Thermo Fisher). All the remaining procedures of single cell including the library construction were performed according to the standard manufacturer's protocol (Chromium Single Cell 5ʹ v2).