title: GSM6514573: CSF, sample 36; Homo sapiens; RNA-Seq
library_construction_protocol: CSF was centrifuged at 2000 g for 20 minutes at room temperature and stored at -80°C. 10 mL frozen aliquots were thawed on ice and diluted 1:1 in PBS. Extracellular vesicles were isolated by ultracentrifugation at 100,000 g for 70 minutes at 4°C, resuspended in 100 µL of PBS supplemented with 1% DMSO, and stored at -80°C. RNA was extracted using the miRNeasy Micro Kit according to the manufacturer's protocol (Qiagen, ID: 217084). cDNA was prepared according to a standard protocol for Illumina RNA sequencing.