title: GSM8009036: colonic epithelium, wildtype, DSS treated, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Epithelium was isolated from the whole colon using 5mM EDTA/PBS buffer and rocked for ~ one hour to seperate the epithelium from the mesenchyme. The isolated crypt epithelium was washed thrice with PBS, pelleted and solubilized in Trizol for RNA extraction. All the aforementioned processes were carried at 4 degrees Celsius. The quality for the RNA was confirmed by Novogene prior to sequencing. The PolyA enrichment procedure was used for library preparation