title: GSM7135797: LNCaP_SMARCD2_IP_rep1_ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% methanol-free formalin for 10 min at room temperature with gentle shaking. After lysis and nuclear enrichment, immunoprecipitation was carried out using HOXB13 (Cat # ab227879, Abcam), SMARCD2 (Cat # ab221168, Abcam) -conjugated protein G Dynabeads (Cat # 10003D, Invitrogen) overnight at 40C. After washing, the DNA was eluted and de-crosslinked at 650C for 8 hours. The DNA was purified using a Monarch DNA purification kit (Cat # T1030, NEB) and quantified by a Qubit high-sensitivity DNA kit (Cat # Q33230, Invitrogen). DNA (5ng) was used for sequencing library construction using NEBNext Ultra II DNA Library Prep Kit for Illumina (Cat # E7645, NEB) according to the manufacturer's instructions. The library was amplified for 10 cycles and quantified by Bioanalyzer (Agilent) and sequenced using Illumina NovaSeq 6000.