title: GSM7847264: Foxf1_HA_ChIP_Rep_1; Mus musculus; ChIP-Seq
library_construction_protocol: 5 × e6 cells were crosslinked with 1% formaldehyde for 10 min and quenched with 0.125 M glycine for 5 min at room temperature. Fixed cells were incubated in 0.5 ml lysis buffer for 10 mins on ice. After adding 1 ml dilution buffer, chromatins were sonicated into 200-800 bp fragments using Bioruptor (Diagenode) and immunoprecipitated with protein A agarose beads and specific antibody at 4 ? for 12 h. Immunoprecipitates were washed with Wash Buffer I, Wash Buffer II, Wash Buffer III and TE, respectively. After the final wash, DNA was eluted and reverse-crosslinked at 65?°C for at least 6 h. DNA was then purified and used for PCR amplification or ChIP-seq library preparation. ChIP-seq libraries were prepared with VAHTS Universal DNA Library Prep Kit for sequencing. The ChIP-seq libraries were prepared with VAHTS Universal DNA Library Prep Kit for Illumina V3