title: GSM6433716: WT_Con2_IP; Homo sapiens; RIP-Seq
library_construction_protocol: Total RNA was extracted from FTO knockdown GC cells and its negative controls. MeRIP-seq was carried out with LC-BIO Biotech (Hangzhou, China). Quality and quantity of total RNAs were evaluated with NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA). RNA integrity was assessed with Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA) with RIN number>7.0 and confirmed by electrophoresis with denaturing agarose gel. Poly(A) mRNA was purified and fragmented into approximately 100-nt fragments. Cleaved RNA fragments were then incubated with an m6A-specific antibody (Synaptic Systems, Goettingen, Germany) in IP buffer at 4°C for 2h. Eluted m6A-containing fragments and untreated input control fragments were converted and stored in our final cDNA library. Paired-end 2×150bp libraries were sequenced on an Illumina Novaseq 6000 platform (LC-Bio Technology CO., Ltd., Hangzhou, China).