title: Homosapiens
design: All sequencing was performed on the Illumina Novaseq 6000 sequencing platform. To obtain clean data, quality control was conducted using the fastp software. Subsequently, clean reads were aligned to the reference genome from the Ensembl database using HISAT2 software. The expression analysis of genes or transcripts was carried out using the RSEM software by quantifying the expression levels through reads counting of clean reads within the genomic regions. Gene transcripts expressing were identified based on the TPM method.