title: GSM7094166: folr-1(syb4116) rep2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol Reagent (ThermoFisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Library construction and sequencing was done by Novogene.