title: GSM7093918: BM adipo and stroma 2, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Collected bone marrow adipocytes and sorted CD45/Ter119/CD31- cells from the bone marrow of Plin1-creER; tdTomato mice 5 weeks after sublethal irradiation were kept frozen until nuclei isolation. Frozen cell pellets were resuspended in TST buffer (10 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM CaCl2, 21 mM MgCl2, 0.03% Tween-20, 0.01% BSA) and incubated for 10 min on ice. After adding ST buffer (10 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM CaCl2, 21 mM MgCl2), solution was filtered through a 40 µm nylon mesh, and then centrifuged (500 g, 5 min) to obtain nuclei. We analyzed 4 Plin1-creER; tdTomato mice (two were pooled together for a total of two samples). Isolated nuclei were loaded into the 10X Chromium system and libraries were constructed using Chromium Single Cell 3' Reagent Kit (v3) according to the manufacturer's protocol. Libraries were subsequently sequenced on a NovaSeq 6000 Sequencing System (Illumina).