title: GSM8059831: RPS27A_1; Homo sapiens; RNA-Seq
library_construction_protocol: After immunoprecitipation, beads were then resuspended in 300 µl RLT buffer (Qiagen RNeasy kit, Qiagen, Germany) and the RNA was recovered using Qiagen RNeasy kit (Qiagen, Germany). Each of the RNAseq libraries was generated from 50 ng of total RNA free of genomic DNA using the NEBNext Single Cell/Low input RNA Library Prep kit for Illumina (E6420S) and following the protocol for Low input The resulting libraries were subjected to a total of 11 amplification cycles and then purified using Ampure XP 0.9X beads.