title: GSM6840525: 12937_10099_151840_HMH2CBGXK_AP1_MO_Ctl_3; Gallus gallus; RNA-Seq
library_construction_protocol: RNA isolation and RNA-seq library preparation was performed using 500,000 cells per sample. For all experiments RNA was isolated using the RNAqueous-micro total RNA isolation kit (Thermo Fisher Scientific AM1931) according to the kit's protocol. RNA was poly(A) selected using the NEBNext Poly(A) mRNA magnetic isolation module (New England Biolabs E7490). TruSeq-barcoded RNA-seq libraries were generated with the NEBNext Ultra II directional RNA library prep kit (New England Biolabs E7760). Quality control of prepared libraries was conducted using an ABI 3730xl DNA analyzer for fragment analysis. Libraries were sequenced on an Illumina HiSeq X instrument with paired end 100bp reads.