title: GSM7421639: D4_90kPa_Aligned; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent. RNA isolation was performed using Qiagen miRNeasy micro kit (Qiagen) and protocol, then cleaned using Zymo RNA Clean and Concentrator kit. RNA libraries for RNA-seq were prepared with the NEBNext Ultra II RNA library kit for non-directional libraries by Illumina. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size  distribution detection. Libraries were multiplexed and sequenced on a NovaSeq 6000 S4 using 150bp paired-end sequencing.