title: phyllosphere microbiome of Fraxinus excelsior - Neu1_Mix1
design: Total DNA was extracted from 100 mg of the ground plant material using the DNeasy Plant Mini Kit (Qiagen). The V5-V6 region of the 16S rRNA genes was amplified by using primer set 799F and 1115R, which excludes chloroplast and mitochondria DNA of the host plant. The primers contained a heterogeneity spacer along with a barcode sequence.