title: GSM8397744: SUM159_pLVX_repl3; Homo sapiens; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted using RNEasy (Qiagen, Hilden, Germany) and assessed using NanoDrop ND-1000 (Thermo Fisher Scientific, Waltham, MA). The RNA quantity of A260/A280 is between 1.95 to 2. Total of 100 ng RNA was further processed for ribosomal RNA removal using the Epicenter rRNA depletion kit. Ribosomal RNA-depleted samples were subsequently used to generate sequencing libraries using Roche Diagnostics KAPA mRNA HyperPrep Kit. RNA Seq performed in Whitehead Institute for Biomedical Research Genome Technology Core using a NovoSeq 6000 (Cambridge, MA). The RNA sequencing data was aligned to human genome hg38.