title: GSM8285220: OVCAR5 ALDHplus CD133plus Cisplatin RNA rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using Qiagen RNeasy Micro kit according to the manufactuer instructions. For each sample, 100 ng of total input RNA was extracted by Rneasy Micro Kit (Qiagen, Cat # 74004). mRNA was isolated by NEBNext Poly(A) mRNA Magnetic Isolation Module, and RNA-seq libraries were prepared using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs Inc., Ipswich, MA).  All the RNA-seq libraries were checked by using a BioAnalyzer (Agilent Technologies) and then sequenced on an Illumina HiSeq4000 system with single-end, 50-bp read length settings.