title: GSM7426611: LSK, FLT3-ITD/ITD Llgl1+/+ Mx1+, Rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was perfomed using Arcturus PicoPuroe RNA Isolation Kit (Thermo Fisher Scienficif) or TRIzol (Thermo Fisher Scientific) according to the manufacturer's instruction. RNA libraries for RNA-seq were prepared for TP1 using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer's instruction, assessed for size and quantified using the 2100 Bioanalyzer (Agilent Technologies) and Kapa Library Quantification Kit (Illumina) prior to sequencing on the Illumina NextSeq500 platform (75bp single end) and TP2 using SmarTer Ultra Low Input RNA v4 (Takara Bio) and NEBNext ChIP-Seq Library Prep Kit for Illumina (New England Biolabs) following manufacturer's instruction, prior to sequencing on the Illumina HiSeq2000 platform (51bp single end).