title: GSM8145421: E7; Gallus gallus; RNA-Seq
library_construction_protocol: Forelimbs from X different E7 embryos, Y different E9 embryos were dissected and dissociated by collagenase and mechanical treatments. 5000 cells per conditions were loaded into the 10X Chromium Chip with the Single Cell 3' Reagent Kit v3 according to the manufacturer's protocol. Samples were pooled together at each developmental stage (E7 (N=X), E9 (N=Y) ) before making the libraries. mRNA libraries were prepared for sequencing using standard Illumina protocols