title: ITS rDNA amplicon of endogenous seedling stage replicate 9
design: DNA for each sample was extracted with FastDNA SPIN Kit (MP Biomedicals), then V4-V5(ITS1F-ITS2R) and of ITS rDNA was amplified by two-step PCR.    Finally, the amplicons were sequenced on Illumina MiSeq PE300 platform in pair-end 300 bp mode