title: GSM8098594: 66cl4_chitin+anti-PD-1_tumor2; Mus musculus; RNA-Seq
library_construction_protocol: Primary tumors were removed, flash frozen in liquid nitrogen, and RNA was isolated using the Rneasy Mini Kit (QiAgen, Valencia, CA, USA) according to the manufacturer's instructions. 250 ng of total RNA was subjected to a stranded library preparation. RNA libraries were prepared for sequencing using standard Illumina protocols.