title: GSM7306503: CD8+ T cell, co-cultured with 4T1 cells, Day3, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from purified CD8+ T cells with trizol reagent (Ambion) according to manufacturer instructions  and quantified using Qubit 2.0 (Thermo Fisher Scientific) following the manufacturer's instructions. Libraries were prepared using the NEBNext® UltraTM RNA Library Prep Kit  for Illumina® (NEB, USA) and sequenced in a novaseq 6000 (Illumina) by the deep-sequencing facility at the Novogene.