title: GSM7025417: Control, IFNAR;RelBko, 1h; Mus musculus; RNA-Seq
library_construction_protocol: Collected in Invitrogen™ TRIzol™ Reagent (Cat# 15-596-018) for RNA and was extracted using Qiagen RNeasy Mini Kit RNA was used for Illumina bead Arrays as described (Cheng et al 2017) and for RNA-seq as described (Sen et al 2020). Briefly, libraries were prepped using KAPA Stranded mRNA-Seq Kit Illumina® platform KR0960 – v3.15 using 1µg of RNA per sample measured using Qubit 2.0 fluorometer. Final libraries were checked via agarose gel and multiplexed with a maximum of 24 samples per sequencing reaction. Libraries were sequenced using Illumina HiSeq 3000 with single end 50bp reads