title: GSM7025812: H1 ES-derived salivary gland epithelial progenitors,rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from CFTR knockout SGEPs and wild type SGEP at day 16 using RNeasy mini kit (Qiagen) following the manufacturer's instructions. RNA concentration and quality were measured by NanoDrop 2000 and the Agilent Bioanalyzer 2100 system. Sequencing libraries were prepared using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.