title: Raw nanopore sequencing read
design: Genomic DNA extraction by GenElute Bacterial Genomic DNA Kit. Long read genomic DNA libraries were prepared with Oxford Nanopore SQK-RBK004 kit and/or SQK-LSK109 kit with Native Barcoding EXP-NBD104/114 (ONT, United Kingdom) using 400-500ng of HMW DNA. Barcoded samples were pooled together into a single sequencing library and loaded in a FLO-MIN106 (R.9.4.1) flow cell in a GridION (ONT, United Kingdom).