title: GSM7213939: Reactive_E; Mus musculus; RNA-Seq
library_construction_protocol: Resting and reactive astrocyte cultures were lysed in TRIzol followed by purification and elution of RNA using phenol-chloroform extraction and the RNeasy Mini Kit. Libraries were prepared following protocols from NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra RNA Library Prep Kit for Illumina. Samples were enriched for mRNA using oligo(dT) beads, which were fragmented randomly and used for cDNA generation and subsequent second-strand synthesis using a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I. cDNA libraries then went through terminal repair, A-base ligation, adapter ligation, size selection, and PCR enrichment.