title: GSM6466008: K562_GAi_total_RNA-seq_R1_F; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using a RNeasy Plus Mini Kit (Qiagen) and qualified using Qubit Fluorometer (RNA IQ > 7). Ribosomal RNA (rRNA) was removed from total RNA using RiboMinus Human/Mouse Module (Invitrogene, 45-7012). rRNA-depleted RNA (125 ng) was reversely transcribed and processed for RNA-library using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs #E7765S). This process was carried out in the order of fragmentation of RNA, priming reaction using random primers, first-strand cDNA synthesis, second-strand cDNA synthesis, cDNA end repair, adaptor ligation, amplification using adaptor primers for 8 cycles, and purification of amplified fragments using beads.