title: Nanopore sequencing of synthetic modified base library:abasic sites
design: Barcoded split-pool synthesis (BSPS) generation of short synthetic DNA molecules containing five barcodes and a stochastically encoded five-mer of the four standard bases (dA, dG, dC, and dT) and an abasic site.  During synthesis, all reads had standard bases at positions 1,2,4, and 5 of the encoded five-mer.  75% of reads had a deoxyuracil incorporated in position 3, while the remaining 25% had a dT incorporated at p3.  Following synthesis, the library was treated with uracil-DNA glycosylase to convert dU residues to abasic sites.  In reads containing abasic sites, the sites result in basecalling errors and uncallable regions due to the extreme current variance, but these sites can be distinguished by the presence of the corresponding abasic site barcode in barcode position 3 in the read.