title: GSM7426212: 1833shp27-STAT3 ChIP-Rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP assay was performed by ChIP-IT High Sensitivity®(HS) Kit (Avtive motif; 53040)according to kit's instruction. Briefly, after cells were crosslinked with 1% formaldehyde, the cells were lysed and sonicated. For the immunoprecipitation, sonicated samples were incubated with p27 (Cell Signaling; 3686s), STAT3 (Cell Signaling; 12640s), cJun (Cell Signaling; 9165s), CBP (Cell Signaling; 7389s), H3K27ac (Cell Signaling; 8173s), SIN3A (Abcam; ab3479), YY1 (Abcam; ab12132), HDAC1 (Santa Cruz; sc-7872), IgG Rabbit (Cell Signaling; 2729s), and IgG mouse (Santa Cruz; sc-2025) antibodies overnight at 4°C. Samples were collected on A/G plus-agarose. DNA was eluted after washing of the beads and decrosslinking. Eluted DNA was used for sequencing. The ChIP DNA library was made using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads kit (NEB; E7103S) per kit's instruction.