title: Intracellular phenotype screen for DNMT1 in EdU positive cells (treated with 5-AzadC)
design: Genomic DNA was isolated from sorted cells using a DNA mini kit (Qiagen) with the lysis step occurring overnight at 56 o C for decrosslinking.The gene trap insertion sites were amplified using a LAM-PCR procedure as described (Blomen et al., 2015). Using a biotinylated primer in the gene trap cassette, single-stranded DNA (ssDNA) products were generated for 120 cycles, captured on magnetic beads, a pre-adenylated ssDNA linker ligated to the 3 end, and a final round of exponential amplificationusing a primer containing Illumina sequencing compatible overhangs at the end of the LTR andin the ssDNA linker.