title: PacBio data for Malus domestica 'WA 38' Genome
design: Approximately 10 grams of young leaf material was harvested from the Malus domestica WA 38 mother tree at the Washington State University and USDA-ARS Columbia View Research Orchard near Orondo, WA, USA and flash-frozen in liquid nitrogen. High molecular weight DNA was isolated using using a Circulomics Nanobind Plant Nuclei Big DNA kit (Baltimore, MD), with 4 g of input tissue and a 2 hour lysis. DNA was tested for purity via spectrophotometry, quantified by Qubit dsDNA Broad Range, and sized on an Agilent Femto Pulse. DNA was sheared with a Diagenode Megaruptor and size-selected to roughly 25 kb on a BluePippin. A PacBio sequencing library was produced using the SMRTbell Express Template Prep Kit 2.0, and CCS (HiFi) reads were produced on two 8M flow cells.