title: GSM7674895: WT (RNA-seq) rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total leaf RNA was isolated using Trizol reagent (Ambion, 15596018), and the RNA quality was evaluated by electrophoresis on Agilent 2100 Bioanalyzer. The rRNAs were removed from the qualified RNA samples using the Ribo-Zero Kit (Epicentre). The resulting rRNA-depleted RNA was used for preparing the RNA sequencing library with NEBNEXT Single Cell/Low input library Prep Kit (New England Biolabs) RNA libraries for RNA-seq were prepared using NEBNext Single Cell/Low input library Prep Kit following manufacturer's protocol