title: Metabarcoding (ITS2) of Nothofagus obliqua ectomycorrhizal root tips: PP-O replicate 2
design: Genomic DNA was extracted from 2X96 pooled ECM tip samples, primers ITS1F and ITS4 were used to amplify the ITS region, then primers ITS1FI2_A (5'-GAACCWGCGGGGARGGATCA-3') and ITS2_A (5'-GCTGCGTTCTTCATCGATGC-3') were used for library preparation to sequence ITS2