title: phyllosphere mycobiome of Fraxinus excelsior - Neu1_Mix2
design: Total DNA was extracted from 100 mg of the ground plant material using the DNeasy Plant Mini Kit (Qiagen). ITS2 of the fungal rRNA region was amplified by using primer set fITS7 and ITS4 which excludes host plant DNA. The primers contained a heterogeneity spacer along with a barcode sequence.