title: GSM7725915: tumor-excised calvarial tissues from the  scratch-positive (invasive stage) group; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell RNA-sequencing was performed on live cells, mixed CD45+ and CD45– cells in a 1:1 ratio, derived from calvarial tissues of tumor-free control mice and tumor-excised scratch-negative (pre-invasive stage) and scratch-positive (invasive stage) mice 1 week after the implantation of HNM007 cells. The sequencing library was generated using the 10x Genomics Single Cell 3′ v3.1 and subjected to DNBSEQ-G400.