title: GSM6792299: 73542 (PLX5622 Rep. 3); Mus musculus; RNA-Seq
library_construction_protocol: CD11b positive cells were isolated according to MACS cell separation protocol (Miltenyi Biotec). Total RNA extraction was carried out using QIAzol lysis reagent and RNeasy Mini Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. Libraries were prepared from 300 ng of input material (total RNA)using NEBNext Ultra II Directional RNA Library Preparation Kit in combination with NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext® Multiplex Oligos for Illumina®(Index Primers Set 1/2/3/4) following the manufacturer's instructions (New England Biolabs).