title: GSM6641996: KI_CNS_infiltrating_Tcells_mouse1; Mus musculus; RNA-Seq
library_construction_protocol: Live CD45iv—CD11b—CD45+CD3+ cells were sorted on a FACSAria (BD Biosciences). Cells were resuspended in PBS 2% FBS and counted on a hemocytometer, before being loaded on the 10x genomics instrument using the Chromium Single Cell 5' kit v2 (10x genomics). Single-cell libraries were generated according to the manufacturer's protocol (10× Genomics).  Single-cell library size and quality were confirmed on the Fragment Analyzer (Agilent). KAPA Quantification Kit for Illumina platforms (KAPA Biosystems, Roche) was used to quantify libraries. The libraries were sequenced on a NovaSeq (Illumina) in pair-end sequencing 150bp