title: GSM7504938: Day 5, Kras, Fra1KO,snATAC, rep 1; Mus musculus; ATAC-seq
library_construction_protocol: Nuclei were extracted from an adapted protocol. 2x salt-Tris solution (ST Buffer) containing 292mM NaCl ( 20 mM Tris-HCl pH 7.5, 2 mM CaCl2  and 42 mM MgCl2in Ultrapure water was mixed in a 1:1 ratio with 2x Tween-based lysis buffer to make TST (1 ml of 2× ST buffer, 60 µl of 1% Tween-20 (Promega, H5152), 10 µl of 2% BSA and 930 µl of nuclease-free water) or with a 2x salt BSA buffer to make ST-SB (1 ml of 2× ST buffer, 133ul of 30% BSA , 40ul of 1% Tween-20, 827ul PBS). Library construction was performed with the Chromium Single Cell ATAC kit