title: GSM8009008: E14 retina-PBS; Mus musculus; RNA-Seq
library_construction_protocol: Cells were dissociated using Accutase (Innovative Cell Technologies) for 15-20 minutes on day 5. Cell pellets were resuspended in PBS containing 0.04% bovine serum albumin and filtered through a 40μm cell strainer (Falcon, Cat#352340) to remove cell clumps. Cells were strained through the cell strainer and counted with a hemocytometer. 10,000 cells were then input into the 10X protocol. GEM generation, reverse transcription, cDNA amplification, and library construction steps were performed according to manufacturer's instructions (Chromium Single Cell 3'v1/v2/v3 platform (10X Genomics, Pleasanton, CA). Library construction steps were performed according to manufacturer's instructions (Chromium Single Cell 3'v1/v2/v3 platform (10X Genomics, Pleasanton, CA).