title: GSM7785540: mESC WT Replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from two replicates of WT and Srrm2 heterozygous knockout cells was isolated with TRIzol (Invitrogen) and DNA was degraded with TURBO DNase (Invitrogen), according to the manufacturer's protocol. Cells were loaded on a 40 µm Flowmi™ Tip Strainer to remove cell debris and clumps into a suspension of 10^6 cells/ml in PBS + 0.04% BSA. Single-cell mRNA-seq libraries were prepared with the Chromium Single Cell 3' v3 chemistry Kit (10x Genomics), according to the manufacturer's instructions. Briefly, a droplet emulsion was generated in a microfluidic chip followed by barcoded cDNA generation inside these droplets. Purified and amplified cDNA was then subjected to library preparation, and sequenced on a NovaSeq 6000 instrument (Illumina).