title: NextSeq 500 paired end sequencing; RNA-seq of anaplastic thyroid carcinoma cells (CAL62 and 8505c) after E2F7 silencing
design: RNA-seq of anaplastic thyroid carcinoma cells (CAL62 and 8505c) after E2F7 silencing
library_construction_protocol: Cells were collected after 48 hours from transfection CAL62 were cultured in Dulbecco's Modified Eagle Medium (DMEM)  added with 10% fetal bovine serum (FBS) Cells were transfected by inverse transfection Total RNA was purified with Maxwell® RSC simplyRNA Cells (Promega) RNAseq libraries were prepared by Illumina TruSeq Stranded Total RNA kit starting from 500 ng of RNA. Libraries quality and quantity were evaluated by Agilent 2100 Bioanalyzer system and Qubit fluorometer (ThermoFisher) respectively.