title: GSM7135556: MII,ZcKO,rep3; Mus musculus; OTHER
library_construction_protocol: Mice were sacrifised on the indicated times and total RNA were extracted from WT and Nat10-ZcKO at GV and MII oocytes for ribo-seq;Mice were sacrifised on the indicated times and total RNA were extracted from WT GV oocytes for acRIP-seq Ribo-seq libraries,briefly, the oocytes were directly lysed in the lysis buffer. Then RNase treatment by RNaseI. Extract RNA by Trizol. End repair, 3' ployadenylation and the full-legnth first-strand cDNA was generated using the riboRT primer, along with the TSO primer. The PCR products were amplified.PCR Library purification using selection beads.Library was performed according to the m6A acRIP-seq method. Briefly, the oocytes were directly lysed in the lysis buffer. acRIP-seq libraries, fragment RNA and incubate with beads with ac4C antibody or IgG antibody. Extract RNA by Trizol. End repair, 3' ployadenylation and the full-legnth first-strand cDNA was generated using the riboRT primer, along with the TSO primer. The PCR products were amplified.PCR Library purification using selection beads.