title: Illumina NovaSeq 6000 paired end sequencing of SAMD00731528
library_construction_protocol: 109 ng of total RNA samples, which met the quality guideline RIN: 8 or higher, were treated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Massachusetts, USA) to enrich poly-A mRNA and remove rRNA molecules. cDNA synthesis followed by transcriptome library preparation were conducted by the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, where dUTP was incorporated in the process of the 2nd strand cDNA synthesis instead of dTTP, which blocks PCR amplification against the 2nd strand templates. This enables us to maintain the strandness of RNA transcripts. A 13-cycle PCR amplification was performed to increase library yield and to incorporate sample barcodes into the library fragments.