title: Long Read RNAseq of Homo Sapiens: Dorsolateral Frontal Cortex (Brodmann Area 9/46)
design: solated RNA was kept on ice until quality control testing was completed as described above. Long read cDNAlibrary preparation commenced, utilizing the Oxford Nanopore Technologies PCR-amplified cDNA kit (OxfordNanopore Technologies, catalogue number: SQK-PCS111). The protocol, though not available due to a legalembargo, was followed, with two notable modifications being that in the cDNA amplification PCR the expansiontime was set to 6 minutes and the PCR was 14 amplification cycles.