title: GSM6700936: MDA PCa 2b; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from MDA PCa 2b and RC77 T/E cells was isolated using TRIzol extraction (Invitrogen, Waltham, MA), isopropanol-precipitated with GlycoBlue (Fisher Scientific, Pittsburgh, PA) and quality assessed using an Agilent 2100 Bioanalyzer system where RIN scores of all isolated RNA were > 9.0. Paired-end library of the prostate cancer cell lines was constructed using NEBNext Ultra Directional RNA kit.