title: GSM7847280: E7.0_Hand1 CKO, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: To prepare single cells for snRNA-seq, embryos at E7.0 were dissected in cold sterile 1×PBS without Ca2+, Mg2+ under a stereo microscope. Embryos were staged based on their morphology. The Reichert?s membrane and ectoplacental cone were removed, and genotypic identification of embryos was carried out. Embryos were placed into a 1.5 ml microfuge tube and digested into single cells using TrypLETM Express (Thermo) at 37°C. snRNA-seq was performed on single-cell suspensions using 10× Genomics.