title: LDNDS114
design: Fecal DNA was extracted using PowerLyzer PowerSoil DNA isolation kit (Qiagen) via bead beater disruption option as manufacturers recommendation. The V3-V4 hypervariable region of the 16S rRNA gene was amplified using primer pair 341F (5- CCTACGGGNGGCWGCAG-3) and 785R (5-GACTACHVGGGTATCTAATCC-3).