title: GSM8242171: ATAC H3K4M, rep2; Mus musculus; ATAC-seq
library_construction_protocol: DNA was extracted using MiniElute Kit according to the manufacturer's instructions. ATAC-seq was performed as previously described (Buenrostro et al., 2013). 60,000 cells were washed once with 100μl PBS and resuspended in 50μl lysis buffer (10mM Tris-HCl pH 7.4, 10mM NaCl, 3mM MgCl2, 0.2% IGEPAL CA-630). The suspension of nuclei was then centrifuged for 10min at 500 g at 4°C, followed by the addition of 50μl transposition reaction mix (25μl TD buffer, 2.5μl Tn5 Transposase and 22.5μl Nuclease Free H2O) and incubation at 37°C for 30min. DNA was isolated using MiniElute Kit (QIAGEN). Libraries were amplified by PCR (13 cycles). After the PCR reaction, the library was selected for fragments between 100 and 1000bp with AmpureXP beads (Beckman Coulter). Integrity for libraries was checked on a TapeStation before sequencing.