title: GSM8417388: Control EPC2-hTERT ALI; Homo sapiens; RNA-Seq
library_construction_protocol: On day 14, the upper chamber was lysed using Tri Reagent (Sigma-Aldrich), and the cell extract was stored at -80°C until further use. RNA was extracted for traditional RNA sequencing using RNeasy Mini Kit (Qiagen) according to the manufacturer's protocol. RNA sequencing libraries of ALI were prepared using the Illumina TruSeq RiboZero kit, followed by single-end sequencing on the Illumina NovaSeq 6000 platform.