title: HI-C seq: of Lupinus luteus: young leaf
design: The nucleic acid extractions were performed from young leaves (~60 mg). According to manufacturers instructions, DNA extraction was performed with Quick-DNA HMW MagBead and Quick-DNA Plant/Seed Kits (Zymo Research).  A Hi-C library was generated as follows. (i) Fresh, young leaves were treated with formaldehyde to fix the conformation of DNA. (ii) Cells were lysed, and cross-linked DNA was digested by the restriction enzyme DpnII. (iii) Digested fragments were ligated and biotinylated. (iv) Purify and fragment the DNA in random fragments into ~300-500 bp. Finally, the Hi-C library was also sequenced based on the Illumina NovaSeq 6000 platform in PE150 mode