title: Illumina NovaSeq 6000 paired end sequencing; Host nasopharyngeal transcriptome analysis in a SARS-CoV-2 positive Italian cohort
design: Host nasopharyngeal transcriptome analysis in a SARS-CoV-2 positive Italian cohort
library_construction_protocol: Nasopharyngeal swab samples from patients from Campania region hospitals in Italy, selected after confirmed SARS-CoV-2 infection by PCR testing. All samples were subjected to total RNA extraction using ELITeInGenius SP RNA cartridge (ELITechGroup) via ELITeInGenius fully automated system (ELITechGroup). The yield of the extract was determined using the Qubit 2.0 fluorometer assay (Thermo Fisher Scientific, Waltham, MA, USA) and the 4200 TapeStation instrument System (Agilent Technologies, Santa Clara, CA, USA). 100 ng of each RNA sample was used for sequencing library preparation using the TruSeq Stranded Total RNA Sample Prep kit protocol (Illumina Inc., San Diego, CA, USA).