title: GSM7307166: patient# 136-47 scalp fibroblast; Homo sapiens; RNA-Seq
library_construction_protocol: single cell suspension was achieved using human whole skin dissociation kit (Miltenyi, cat# 130-101-540). To achieve live single cell suspension, Propidium Iodide (BioLegend, 421301) negative and Vybrant DyeCycle Violet (Invitrogen, V35003) positive single cells were sorted. Library construction was 10x Chromium Single Cell v3. Sequencing was performed on the NovaSeq 6000, v1.5 reagents.  Basecalling and sample demultiplexing is performed within Illumina's BaseSpace software.