title: GSM8145252: WGBS_MECP2_WT_rep2; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Three-week differentiated endogenous-tagged WT MECP2-GFP NGN2-neurons were collected for the genomic DNA extraction using the Quick-gDNA MicroPrep (Zymo D3020). Bisulfite conversion and library preparation were performed using Pico Methyl-Seq Library Prep Kit (Zymo D5455) according to the manufacturer's instructions. Libraries were pooled and sequenced on NovaSeq 6000 (Illumina) on a paired-end 100x100 run with ~650-800 million reads.