title: GSM7504929: Day 5, Kras, IgG, CUT&RUN, rep 3; Mus musculus; OTHER
library_construction_protocol: Acinar-lineage cells were sorted for YFP+/DAPI- signal and  were fixed in 0.1% formaldehyde for 2 minutes before quenching with 125mM glycine. Afterwards, cells were washed in wash buffer (20mM HEPES-KOH, pH 7.9, 150mM NaCl, 1% Triton X-100, 0.05% SDS) and proceeded to Concanavalin-coated magnetic bead binding as described previously using 0.01% digitonin for all buffers. H3K27ac (Cell Signaling Technology, 8173S, 1:100) was incubated overnight followed by incubation with secondary antibody, guinea pig anti-rabbit IgG (Antibodies-Online, ABIN101961, 1:100). Rabbit IgG was used as a control (Epicypher, 13-0042). mNase (Epicypher, 15-1016) digestion was carried out following manufacturer's instructions. Chromatin was precipitated and released following the Henikoff protocol Libraries were prepared with NEBNext Ultra II Library Preparation Kit