title: GSM8189174: Rep1, ASO-L1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using the Direct-zol RNA microprep Kits (Zymo), according to the manufacturer's instructions. 200ng equal amount RNA each sample was used to prepare sequencing library. The cDNA library was prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #  E7490S) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB # E7760L), following the manual instruction. Library cDNA were equally pooled at 10 nM for sequencing.