title: ddRADseq of Rana luteiventris
design: Tissue samples from Columbia spotted frogs (Rana luteiventris) collected from 31 sites in Nevada (USA) and sequenced using ddRAD sequencing on 10 lanes of an Illumina HiSeq4000. Data processing of uploaded files includes: (1) removal of PCR duplicates using Stacks v. 2.41 clone_filter [flags: --null_inline --oligo_len_2 10]; (2) removal of degenerative barcodes and quality screening using Trim Galore! v. 0.6.4 [flags: --paired -q 20 --stringency 6 --clip_R2 10]; (3) demulitplexing, read trimming, and other quality screening using Stacks v. 2.41 process_radtags [flags: --renz_1 'nspI' --renz_2 'ecoRI' --inline_null -t 90 -c -q -r -E 'phred33']. For complete methods information see Forester et al. (2022) Genomics-informed delineation of conservation units in a desert amphibian, Molecular Ecology.