title: GSM7785034: WT, embryo, 26°C, ATAC-seq rep1; Caenorhabditis elegans; ATAC-seq
library_construction_protocol: The live embryos are incubated in chitinase solution and slowly rotated at room temperature for 0.5-1 hours to digest egg shells. embryos were dissociated into single cells by pipetting up and down and resuspended in ATAC lysis buffer incubated at 4℃ for 10 minutes to break cell membrane and release nuclei. Nuclei were used for library preparation directly by Vazyme TruePrep DNA Library Kit (Vazyme), according to the manufacturer's protocol.