title: RNA-seq of human periodontal ligament tissue during orthodontic tooth movement
design: the Lexogen QuantSeq3 mRNA-Seq library prep kit (Lexogen, Vienna, Austria) was applied according to the manufacturers protocol with 500 ng input. Following preparation, the libraries were measured with Qubit and placed on the Fragment analyzer to guarantee they could be pooled equimolar to 2 nM. The pool was subsequently quantified using RT-qPCR, and a final pool (2nM) was created for single-end 50-base pair (bp) sequencing on the Illumina Inc. HiSeq4002.