title: RNA-seq of T. vanhyningii at Night, Drought (clonal accession D)
design: The experiment comprised six clonal which were placed under identical greenhouse conditions. Prior to the experiment, the accessions were allowed to acclimate to greenhouse conditions for 6 weeks, before being placed in a regime of 12 hours of light / 12 hours of dark for 14 days, by complementing daylight with artificial growing lights. During this time, three accessions of each species were placed in a drought regime for 14 days by not watering. The remaining three accessions were kept in normal watering conditions used to maintain the plants in the botanical garden (1x every two days, control). After 14 days, all accessions were sampled every 6 hours (Four timepoints: Day + 4 hours (10 am), Day + 10 hours (4 pm), Night + 4 hours (10 pm), Night +10 hours (4 am)), generating three replicates for the combination of treatments (species x time point x watering regime. RNA-seq data was obtained for samples at timepoints 10 am (D+4) and 10 pm (N+4) for two watering regimes and two species (24 samples in total). Total RNA was extracted using the QIAGEN RNeasy Mini Kit. RNA library preparation and poly-A capture were performed at the Vienna Biocenter Core Facilities (VBCF) using a NEBNext stranded mRNA kit. The libraries were sequenced on one lane of Illumina NovaSeq SP 150 PE flowcells.