title: GSM8416066: KO, B1-a cell，24month, male, rep2; Mus musculus; RNA-Seq
library_construction_protocol: 5000 B-1a cell were isolated into lysis buffer,then  the process involves the following steps: Reverse transcription of RNA into cDNA; Amplify and purify the cDNA; Fragment cDNA with Tn5 enzyme and construct the library ; two replicates of each sample were done. 5000 cells analysed per experiment. The purified cDNA samples were added to TN5 buffer and TN5 enzyme-1, and the reaction was interrupted according to the program. After the reaction, mix Smart Amp buffer-2, Smart Amp enzyme-2 for library construction, purify and send for testing.Illumina NovaSeq 6000 sequencing platform was usually used, and PE150 sequencing mode was adopted.