library_construction_protocol: Using primers published in the 16S Metagenomic Sequencing Library Preparation Protocol, sample DNA was utilised to run PCR. DNA amplicons were purified using magnetic beads. The library was double-indexed using the Nextera XT kit and cleaned up using magnetic beads. Then it was quantified using the Qubit. The size of the library (640–650 base pairs (bp)) was checked using TapeStation. The final concentration of library was 50 pM. Sequenced using the iSeq 100 i1 Reagent v2 Kit on the iSeq 100 Instrument (Illumina). 10% of PhiX viral DNA was added to the sample pool as an internal control.