title: GSM8100730: tpn-1,scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Freshly 5 cm of mouse jejunum tissue was excised and longitudinally opened and Peyer's pathes removed, and resuspended in RPMI 1640 solution (10% FBS added) into gentleMACS C tubes. To digest the tissues, DNase I (DN25, Sigma-Aldrich) at 1:2000 dilution, Protease Inhibitor Cocktail (HY-K0010, MedChemExpress), Dispase II (40104ES80, Yeasen) and Liberase (5401054001, Roche) at 1:37.5 dilution were added. The tissue was then digested using a gentleMACS Dissociator (Miltenyi Biotec). The obtained single cell suspensions were counted and quality checked, and the gel beads with barcodes and primers were encapsulated with individual cells in oil droplets to form a GEM (Gel bead in emulsion) through the Chromium™ Single Cell 3' Solution microfluidic platform. The cDNA with barcode was obtained by reverse transcription, amplified and purified for quality check, and the cDNA that passed the quality test was used for library construction.