title: GSM7504839: Control_NPC_day6_scRNAseq_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: NPCs were collected using Accutase and single cell suspensions were prepared at a concentration of 0.8-1.2x106 cells/ml and with cell viability of 80% or above. Briefly, approximately 5000 cells were used together with the Chromium Single Cell 3' Library & Gel Bead Kit v3.1 (10x Genomics, 1000268) onto the 10x single cell chromium chip to generate a single cell emulsion in Chromium Controller (10x Genomics, 120263). Reverse transcription of mRNA and cDNA amplification were carried out following the manufacture's instruction (10x Genomics, 1000268). The amplified cDNA was further fragmented to construct NGS libraries.