title: GSM7137690: RNA-seq shCTR9 Dox rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using peqGOLD TriFast (PeqLab). Total RNA was extracted using the miRNeasy kit (QIAGEN) including on-column DNase I digestion. mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) and library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the instructions. Libraries were size selected using Agentcourt AMPure XP Beads (Beckman Coulter), followed by amplification with 11 cycles of PCR.