title: GSM8416725: H3K27me3_AEBP2KO_Rep1; Mus musculus; OTHER
library_construction_protocol: For RNA-Seq: Cell pellets from WT ESCs were collected at different days (representing Replicates 1,2 and 3) and total mRNA extracted using the Qiagen RNeasy Mini Kit. ChIP-Seq/Rx library preparation was performed using the NEBNext Ultra II DNA Library Kit for Illumina (E7645) according to the manufacturer's protocol. The CUT&RUN experiments were performed following the Epicypher CUT&RUN Protocol v1.5.1. The Monarch PCR & DNA Cleanup Kit (New England Biolabs) protocol was used to purify the DNA. RNASeq Libraries were prepared by Novogene and sequenced using NovaSeq X Plus PE150. Illumina NextSeq 500, 75-bp single end (rescues samples); NextSeq2000, 75-bp single end (AEBP2 KO ChIPs); NextSeq2000, 50-bp paired-end