title: RNA-Seq of mus musculus: adult female colon
design: Germ-free C57BL6 mice were gavaged with an Akkermansia muciniphila transposon mutant in amuc_0544. After 14 days, colon tissue was collected and stored in RNALater (Invitrogen). RNA was isolated using a Direct-zol RNA miniprep kit (Zymo) and the quality was assessed on an Agilent Bioanlyzer. Poly-A purification of mRNA was carried out using a NEBNext Poly(A) mRNA Magnetic Isolation Module and used to prepare libraries with the NebNext Ultra II directional RNAseq kit (NEB). Sequencing was performed at the Duke Sequencing Core Facility.