title: GSM8189779: NSC, YY1, ChIP, rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Briefly, 10 ng of ChIP DNA were end-repaired, dA tailed and adaptor ligated using NEBNext ChIP-seq library preparation kit (E6240, NEB), according to manufacturer's instructions. Adaptor ligated DNA was eluted into 32 µL of 0.1xTE, 25 µL were used for ChIP-seq library preparation and 5 µl were processed for ChIP-STARR-seq plasmid libraries preparation. ChIP-STARR-seq; For ChIP-seq library preparation, purified adaptor ligated DNA was PCR amplified with Illumina index primers (E7335 and E7500, NEB) according to manufacturer's instructions. ChIP-seq libraries were assessed on an Agilent Tapestation. All sequencing occurred on an Illumina HiSeq 2500 platform, using 50bp single-end sequencing.