title: GSM6514361: KO-MAP4K5-099-4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from fresh cell pellets using the Qiagen RNeasy Plus Mini Kit (Qiagen: 74136), according to the manufacturer's instructions. RNA-Seq library preps were constructed with the Illumina TruSeq® Stranded mRNA Library Prep kit (Cat #20020595) using 1000ng of total RNA as input, amplified by 10 cycles of PCR, and sequenced paired-end 50 cycles on an Illumina NovaSeq6000 SP flowcell with a 2% PhiX spike-in.