title: GSM7673636: Mouse Thymus (APC), Perinatal Day 28, Wild-type, Hashtagged Sample (GEX); Mus musculus; RNA-Seq
library_construction_protocol: To sort hematopoietic APCs for scRNA-sequencing, thymi were harvested and enzymatically digested with an enzymatic solution comprising  LiberaseTM (Roche) and DNAse I in PBs at 37 degrees celsius. Isolated cells were spun down and stained with the corresponding panel of antibodies. The sort samples were resuspended in FWB+ EDTA buffer plus propidium iodide (Enzo). Sorted hematopoietic APCsells were then spun down in FWB without EDTA, counted and resuspended at a concentration of 700-1200 cells/µL prior to submission to the sequencing core. Library prep was performed per the manufacturer's protocol for scRNA-seq samples (Chromium Next GEM Single Cell 3' v3.1).