title: RNA-Seq of untreated Shigella flexneri
design: The library preparation was carried out using Trueseq standard total RNA (Illumina #15035748). Final libraries were quantified using Qubit 4.0 fluorometer (Thermofisher #Q33238) using DNA HS assay kit (Thermofisher #Q32851) following manufacturers protocol.  To identify the insert size of the library, we queried it on Tapestation 4150 (Agilent) utilizing high sensitive D1000 screentapes (Agilent # 5067-5582) following manufacturers protocol.