title: GSM6736308: Src CD library at 19 hours, rep2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Plasmid extraction from culture samples using Yeast Plasmid Prep I (Zymogen) according to manufacturer's protocol Extracted barcodes were amplifed and sample indices appended using 2x KAPA2G Robust HotStart ReadyMix with Thermocycler conditions of: Initial denaturation at 95ºC for 3 min, followed by 17 cycles of 95ºC for 15s, 60ºC for 15s, and 72ºC for 15s. Amplicons were quantified using KAPA Library Quantification Kit.