title: GSM7504951: TNFR2KO-2; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were harvested, and a single-cell suspension was generated following red blood cell lysis and filtration through a 70-uM filter. NK cells were enriched from the spleen by negative selection using the Mojosort Mouse NK cell isolation kit. Ly49H+ (CD45.1 or CD45.2) cells were sorted with the Sony SH800. RNA was extracted using the purelink RNA mini kit (Thermofisher). Poly-A enriched (stranded) library. Performed by Genome Quebec in Montreal, Quebec, Canada. RNA-Seq perfomed using 100bp paired-end sequencing by Novaseq 6000. Performed by Genome Quebec.