title: GSM8190547: Wild-type, RAW264.7, 0h, bio rep 2; Mus musculus; ATAC-seq
library_construction_protocol: 50000 cells were lysised in 10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2. Cell nuclei were harvested. ATAC-seq libraries were prepared by  the Hyperactive ATAC-Seq Library Prep Kit for Illumina (TD711, Vazyme), complemented with TruePrep Index Kit V2 for Illumina. 50 ng of DNA was  using as starting material. Libraries were amplified using 9 cycles on thermocycler. Post amplification libraries were sized selected at 250-450bp using VAHTS DNA Clean Beads. Libraries were valided using Agilent2100 Bioanalyzer.