title: GSM8241471: CD45-/CD45+, PDG-Ink4a Recurrent, c; Mus musculus; RNA-Seq
library_construction_protocol: The Chromium Controller platform of 10X Genomics was used for single cell partitioning and barcoding. Each cell's transcriptome was barcoded during reverse transcription, pooled cDNA was amplified and Single Cell 5' Gene expression libraries were prepared according to the manufacturer's protocol (CG000207 Rev E and CG000331 Rev A, 10X Genomics). The Single Cell 5' Gene Expression libraries were pooled to create three sequencing pools. The sequencing pools were quantified by qPCR, according to the KAPA Library Quantification Kit Illumina® Platforms protocol (KR0405, KAPA Biosystems). The Single Cell 5' Gene Expression libraries were sequenced on a NextSeq550 or NovaSeq 6000 system (Illumina) using a NextSeq 500/550 High Output Kit v2.5 (cat. no. 20024906, Illumina) and NovaSeq SP Reagent Kit v1.5 (cat.no. 20028401, Illumina), at a sequencing depth of 20.000 reads pairs/cell.