title: GSM7211465: T2KO-old-LSK-MicroC; Mus musculus; Hi-C
library_construction_protocol: 0.5 million LSK (Lin-cKit+Sca1+) cells were harvested from WT-young, WT-old, T2KO-young and T2KO-old mice micro-C data were generated using Dovetail® Micro-C Kit. In briefly, the cells were crosslinked with FA in the presence of DSG, followed by in situ MNase Nuclease digestion and cell lysis. Then chromatins were captured by chromatin bind beads for future end polishing, bridge ligation, intra-aggregate ligation and reverse crosslinking. The purified DNA were proceeded with library generation.