title: GSM7675556: BE4max opt at CCR5 #1 locus on mPB CD34 edited at day 3 collected at day 7 - rep1; Homo sapiens; OTHER
library_construction_protocol: gDNA was isolated with QIAamp DNA Micro Kit (QIAGEN) according to the manufacturer's instructions. Custom library construction protocol. PCR amplicons for each individual sample were generated by nested PCR starting from >50-100 ng of purified gDNA.The first PCR step was performed using the following amplification protocol: 95°C x 10', (95°C x 30'', 59°C x 30'', 72°C x 20''