title: GSM8144701: NBC_ML95_IL4_4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified using the Trizol reagent followed by RNAeasy column purification (Qiagen). RNA was assessed using the Bioanalyzer (Agilent #5067-1513) and a Qubit RNA High Sensitivity assay (Thermofisher # Q32855), and 2ng of RNA input was processed for sequencing using the Takara SMART-Seq  stranded kit (#634444). The libraries were barcoded, pooled, and sequenced using paired-end, 151 bp sequencing at the University of Michigan Advanced Genomics Core.