title: GSM7720762: Skin, Control-3D-1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of skin tissue was extracted using TRIzol as described previously. Aliquots of mRNA in each group were extracted and purified by the NucleoSpin RNA Plus XS Kit (Macherey-Nagel). Libraries were constructed using the Smart-Seq2 method as previously described and sequenced with 10G depth and paired-end reads on an Illumina NovaSeq 6000, performed by Annoroad Gene Technology.