title: GSM6401810: Conv_C3_19_U5; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 cells were pelleted then lysed to isolate nuclei. For tagmentation, supernatants were removed and nuclei were resuspended in 50uL of Tn5 transposition mix (100nM Tn5 (Illumina, 20034197), 0.01% digitonin, 0.1% Tween-20 in 1x TD Buffer (Illumina, 20034197), and incubated at 37°C for 30 minutes in a thermomixer while shaking at 700 rpm. Tagmented DNA was purified using a Qiagen Min-Elute PCR clean-up Kit (Qiagen, 28004) and PCR amplified for 8-13 cycles using Q5 High-Fidelity DNA polymerase (NEB, M0491S) with primers containing Illumina Nextera adaptors for library generation. The libraries were then size selected (described in (76)) with SPRISelect Beads (Beckman-Coulter, Cat# B23318), and concentrations were measured by a Qubit fluorometer using the Qubit dsDNA HS Assay kit (Life Technologies, Q32851) followed by bioanalyzer verification. Libraries were sequenced on the NextSeq 2000 platform (paired-end, 50 bp read lengths).