title: fxIs1 I; spe-44(fx110[spe-44::degron]);daf2;L4440_rep031523
design: poly-A containing RNA transcripts were converted to cDNA using barcoded oligo-dT primers in the reverse transcription reaction to index each sample following the drop-seq method. The pooled barcoded cDNA samples were purified and amplified by PCR, and then turned into sequencing libraries using the Nextera kit.