title: GSM6514091: SFH_sample273; Mus musculus; RNA-Seq
library_construction_protocol: For Mx1-Cre:FLT3ITD/ITD and control BM, 1x104 to 2x105 LSK cells were sorted into TRIzol® (Thermo Fisher Scientific, Waltham, MA) and RNA was isolated according to the manufacturer`s instruction. cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep kit with the polyA selection module (New England Biolabs) and purified with AMPure beads (Agilent). Libraries were quantified with the KAPA Library Quantification Kit for Illumina (Kapa Biosystems) and pooled. Sequencing was performed on the Illumina NextSeq 500/550 Sequencer High Output mode (1 × 75 bp).