title: GSM8241728: S1 to S14; Mus musculus; RNA-Seq
library_construction_protocol: B6 mice were injected with CR or GPR65 KO tumor cells (i.v.) followed by treatment 7 days later with 10x106 activated CAR-T cells or mock PBS injection (retro-orbital). Spleens were harvested from untreated mice at day 7 and from CAR-T treated mice at day 11 after tumor engraftment. Processed spleens were stained and sorted to isolate CR or GPR65 KO tumor cells (RFP+), and the host immune cell compartment (RFP–Thy1.1–CD45.2+) Tumor and host cells from individual treated and untreated mice were fixed separately using the 10X Genomics CG000478 Demonstrated Protocol Cell & Nuclei Fixation Chromium Fixed RNA Profiling Rev B. After fixation, individual samples were counted and barcoded with unique sample barcodes during probe hybridization. Uniquely barcoded samples were counted using Trypan Blue stain and then pooled in equal numbers following 10X Genomics CG000565 Chromium Fixed RNA Profiling Multiplexed Samples Pooling Workbook Rev B, washed and 8000 cells were targeted from each sample to be partitioned into GEMS on Chip Q using the 10X Genomics Chromium Fixed RNA Profiling kit.