title: sciMETv2.LA preparation of human middle frontal gyrus using three 96-plex tagmentationa dapter plates sequencing run 1.
design: sciMETv2.LA preparation of human middle frontal gyrus using the sciMETv2 protocol for combinatorial indexed single-cell DNA methylation profiling. This method involves fixation of isolated nuclei followed by nucleosome disruption to enable genome-wide access during indexed Tn5 tagmentation using methylated adapters across three 96-well plates, all with unique indexes. Nuclei are then pooled and distributed to new wells of a plate for bisulfite conversion, reverse adapter incorporation, and indexed PCR amplification.