title: GSM6788564: F003-3_Bcell_nonSmoker; Homo sapiens; RNA-Seq
library_construction_protocol: Granulocytes were isolated directly from whole blood using anti-CD15+ antibody–coated magnetic beads following the protocol (Invitrogen). Density gradient centrifugation using Histopaque-1077 Ficoll medium and Accuspin™ Tubes (Sigma-Aldrich) was used to isolate the mononuclear layer of CD19+ B cells using antibody-coated magnetic beads (Invitrogen). RNA was harvested using RNeasy mini plus kit (Qiagen). Aliquots of RNA (375 ng) were used to generate poly-adenylated RNA libraries with TruSeq Stranded Total RNA Ribo-Zero Human Gold kit (Illumina). Samples were indexed with NEXTfle-96 RNA-Seq Barcodes (Bioo-scientific, Austin, TX).