title: GSM6641559: CMO Labels; Mus musculus; RNA-Seq
library_construction_protocol: Animals were humanely euthanized at 7-dpi in accordance with ethics guidelines. TA muscles were pooled from 3 mice according to treatment, then digested into single cell suspensions as described above. Labeling with cell multiplexing oligos (CMOs) (10x Genomics) was performed according to manufacturer's instructions (Demonstrated Protocol CG000391 Rev B, Protocol 3). Each treatment condition (vehicle and maresin 1) was labeled with a separate CMO tag. Then equal cell numbers from each sample was pooled, stained with 7-AAD, and FACS sorted to remove dead cells and debris. Post FACS, 8,000 cells were loaded into the 10x Genomics chromium single cell controller and single cells were captured into nanoliter-scale gel bead-in-emulsions (GEMs). cDNAs were prepared using the single cell 3′ Protocol as per manufacturer's instructions.