title: GSM7135751: epi2; Danio rerio; RNA-Seq
library_construction_protocol: Six adult Tg(spic:EGFP) fish were sacrificed, 8,000 EGFP+ cells, and 50,000 singlet cells from the single-cell suspension of epidermis were sorted into 5 μl 1% BSA/PBS with 0.5 μl RNase inhibitor by the BD Influx™ Cell Sorter. The concentration of sorted cells was measured with the Countess™ II FL Automated Cell Counter (Invitrogen). 10x scRNA-seq library was carried out with a 10X Chromium single cell platform. In brief, after the cell sorting, single-cell suspensions were prepared according to the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 User Guide and loaded onto the 10x Chromium Single Cell Controller to generate single-cell Gel Beads-in-emulsion (GEMs). Libraries were prepared using Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3.1 according to the manufacturer's instructions. Library quality was assessed using an Agilent Fragment Analyzer System, followed by sequencing on Illumina Novaseq 6000 system (Illumina) for a targeting average depth of around 100,000 read pairs per cell.