title: RNA-Seq of Solanum tuberosum: cold stored Irradiated Santana tuber tissue
design: Trizol reagent was used for RNA extraction as per the manufacturers protocol (Sigma, USA). Poly(A) RNA was captured with magnetic oligo-dT beads (KAPA mRNA HyperPrep Kit, Roche, USA). First strand cDNA was synthesized using random primers. 2nd strand cDNA synthesis converted cDNA:RNA hybrid to double-stranded cDNA (ds cDNA), while marking the 2nd strand with dUTP. dAMP was then added to the 3'-end of ds cDNA fragments. 3'-dTMP adapters were ligated to 3'-dAMP library fragments. Adapter-ligated library DNA was amplified by polymerase chain reaction (PCR). Library fragment size distribution was confirmed by electrophoresis. Library concentration was determined by qPCR (KAPA Library Quantification Kit, Roche, USA).Paired End (2X150 bp) sequencing was performed.