title: GSM6433821: imb_keller_2022_03_10_abHA_empty_Rep2; Anopheles gambiae; OTHER
library_construction_protocol: The cell suspension was counted and 0.4 Mio cells were used for each reaction. The experiment was performed following the native protocol dx.doi.org/10.17504/protocols.io.bcuhiwt6. Antibodies are listed with the respective samples. We used pA-Tn5 prepared by the IMB protein production core facilty. CUT&Tag libraries were amplified using NEBNext Ultra II Q5 Master Mix with standard protocols and 15 PCR cycles. Libraries were cleaned up using 1.3 volume Ampure XP beads and pooled together for paired-end sequencing. OTHER: CUT&Tag