title: MiSeq of fungi: plant
design: The extraction of total DNA from surface sterilized shoots, roots, and internodes, as well as from the substrate from control pots, used the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.). The E.Z.N.A. soil DNA Kit comes with detailed instructions that guide researchers through the DNA extraction process. The 18S rRNA gene was PCR amplified using fungal primers ITS1F / ITS2R (Li et al., 2023). The 16S rRNA gene was firstly amplified using bacterial primers 799F /1392R (Bulgarelli et al., 2012; Lundberg et al., 2012), and then using primers 799F/1193R (Bulgarelli et al., 2015). Purified amplicons ran on the Illumina nextseq platform (2  300 bp).