title: Raw illumina sequencing reads
design: Bacterial biomass was sent to MicrobesNG in a TSA plate for genome extraction, library preparation and sequencing. Bacterial suspension was lysed with 120 µL of Tris-EDTA buffer containing lysozyme (final concentration 0.1 mg/mL) and RNase A (ITW Reagents, Barcelona, Spain) (final concentration 0.1 mg/mL), incubated for 25 min at 37°C. Proteinase K (VWR Chemicals, Ohio, USA) (final concentration 0.1 mg/mL) and SDS (Sigma-Aldrich, Missouri, USA) (final concentration 0.5% v/v) were added and incubated for 5 min at 65°C. Genomic DNA was purified using an equal volume of SPRI beads and resuspended in EB buffer (Qiagen, Germany). DNA was quantified with the Quant-iT dsDNA HS kit (ThermoFisher Scientific) assay in an Eppendorf AF2200 plate reader (Eppendorf UK Ltd, United Kingdom).Genomic DNA libraries prepared by Nextera XT Library Prep Kit following manufacturer's protocol Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries were sequenced using Illumina sequencers (NovaSeq6000) using a 250bp paired end protocol.