title: GSM8241179: LV_cKO_M_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Left ventricle tissue (~20 mg) was mechanically homogenised in TRI reagent with a 5 mm steel bead at 30 Hz for 2 x 30 s (TissueLyser II, Qiagen) then centrifuged for 10 min at 10,000 x g. RNA was extracted from the supernatant using spin columns with DNase-I treatment (Zymo DirectZol RNA Miniprep kit #R2050, Integrated Sciences, Australia). RNA concentration and purity was determined spectrophotometrically (Nanodrop, Thermo Fisher, Australia) and RNA integrity number (RIN) was determined by electrophoresis (Tapestation, Agilent). All sample RIN values were ≥7.7. Stranded libraries were prepared from 500 ng total RNA input with poly-A+ selection (TruSeq Stranded mRNA, Illumina) following manufacturer's protocols