title: GSM7674910: BMSCs, Dmp1KOFgf23cKO1; Mus musculus; RNA-Seq
library_construction_protocol: Collected cells were isolated after 5 min Trypsin 1X incubation. Cells were incubated with 7-Aminoactinomycin D (7AAD) for 10 minutes and kept on ice until fluorescence sorting. Live cells (7AAD negative) were  filtered through a 40 µm nylon mesh, and isolated cells were loaded into the 10X Chromium system and libraries were constructed using Chromium Single Cell 3' Reagent Kit (v3) according to the manufacturer's protocol. Libraries were subsequently sequenced on a NovaSeq 6000 Sequencing System (Illumina). Samples were multiplexed using the CellPlex kit (1 separate reaction per genotype) (10X Genomics).The sequencing data were demultiplexed using the Cell Ranger 7.0.1 pipeline  (10x Genomics).