title: GSM7093882: Repressible rne strain in the presence of ATc at 8 minutes after rifampicin treatment replicate 1; Mycolicibacterium smegmatis; OTHER
library_construction_protocol: Pellets were resuspended in Trizol and lysed by bead-beating. RNA was extracted with a Zymo Direct-zol kit, treated with DNase, and purified with a Zymo Clean and Concentrate kit. Libraries were constructed by the Broad Institute Microbial 'Omics Core using the protocol described in https://doi.org/10.1038/nmeth.3313 Ligation of 3' and 5' adapters to sheared RNA to construct paired-end Illumina RNAseq libraries with TruSeq-style adapters