title: CR#2
design: We performed CUT&Tag to analyze the ATF3 binding gene regulatory element differences between the full medium (FM) and cystine-restricted medium (CR). We followed the standard instructions of the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme TD#903, Nanjing, China). PCR mix and adapter (Vazyme TD # 202) were used for library amplification. The fragment library was purified with 1.2 times the volume of DNA magnetic beads (Vazyme #N411).