title: Illumina HiSeq 2500 sequencing; Nucleosome positioning study of Arabidopsis er/erl1/erl2 mutant using MNase-seq approach
design: Nucleosome positioning study of Arabidopsis er/erl1/erl2 mutant using MNase-seq approach
library_construction_protocol: 5-week-old WT and er/erl1/erl2 seedlings growth in long-day condition were used to MNase-Seq.  Mapping nucleosome positioning was performed with 2g samples of WT and er/erl1/erl2. according to Saleh et al. (2008) and Zhu et al. (2013) with minor modifications (i.e. the buffer contained 1% proteinase inhibitor cocktail (Sigma) instead of pepstatin A and aprotinin). 200 Kunitz Units of Micrococcal Nuclease (NEB) were used for digestion of each sample. DNA was purified with phenol:chloroform extraction, ethanol precipitation and size separated on 2% agarose gels. Bands corresponding to ~150 bp were gel isolated, purified and used for library construction. Illumina compatible libraries of MNase-treated gDNA were prepared according to the protocol of Ovation Ultralow DNA Library kit (NuGEN).