title: GSM7308375: Post-transplant, no_aGVHD_rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were removed from liquid nitrogen and thawed in aMEM supplemented with 10% FBS and a DNASe-containing dextran-Albumin solution. We utilized a bead based magnetic separation approach to isolate live cells. Viable cells were counted and then scATAC libraries were prepared using the 10x Chromium Chromium Next GEM Single Cell ATAC Kit v2 (cat # 1000406). These ATAC libraries were technically single index, but were sequenced in a dual index format (50x8x16x50) and were sequenced at a depth of 25-50,000 read pairs per cell on either the Illumina NextSeq2000P2 or Illumina NovaSeq SP.