title: GSM7674097: Human Sperm 18; Homo sapiens; ncRNA-Seq
library_construction_protocol: Human Spermatozoa were purified by centrifuging through a 50% gradient of Puresperm (Nidacon International AB). Total RNA was extracted from spermatozoa with trizol-chloroform phase separation method followed by precipitation and wash steps with 100% and 70% ethanol. RNA purity, integrity and quality was examined by Bioanalyzer (Agilent Technologies) and Qubit (Thermo Fisher Scientific). Sequencing libraries were prepared using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA) with 100-120 ng RNA as a total input.