title: VC_KKV2580 flrB G41D
design: Vibrio cholerae strain KKV2580 contains bcde background with only the FlaA gene. KKV2580 possessed immobility phenotype, however, regained motility upon missense mutation G41D. Strain was cultured overnight in Luria-Bertani broth (37C, 180rpm), and total genomic DNA was extracted using the QIAamp DNA minikit (Qiagen), following the manufacturer's instructions. A paired-end 2  250-bp library was prepared using the Nextera XT DNA library preparation kit (Illumina) and sequenced using the 500-cycle MiSeq reagent kit v2 (Illumina).