title: GSM6459678: NOSN01.HiC, in situ Hi-C; Homo sapiens; Hi-C
library_construction_protocol: Hi-C libraries were prepared manually by performing DNA end repair, removal of un-ligated ends, adenosine addition at 3' end (NEB, M0212), ligation of Illumina indexed adapters (NEB, M2200), and PCR amplification (Thermo Fisher Scientific, F549). The number of cycles for PCR amplification was determined based on KAPA library quantification kit (KAPA, KK4854).