title: GSM8008793: VCaP, ENZ_2; Homo sapiens; ATAC-seq
library_construction_protocol: For bulk ATAC-seq cells were crosslinked with fresh 1% formaldehyde for 10 minutes, then quenched with glycine and frozen. Cells were thawed in cold ATAC-seq buffer (ASB, 10 mM Tris-HCl pH 7.4, 10 mM NaCl, and 3 mM MgCl2) and centrifuged. Cells were lysed by resuspension in .05 mL of lysis buffer (ASB with 0.1% NP40, 0.1% Tween-20, and 0.01% digitonin), followed by addition of 1 mL ASB with 0.1% Tween-20 and centrifugation. Nuclei were resuspended in 50 μL tagmentation reaction containing 25 mL tagmentation buffer (Illumina 15027866), 16.5 mL PBS, 0.5 mL Tween-20, 0.5 mL 1:1 water diluted digitonin, 2.5 mL Tn5 transposase (Illumina 15027865), and 5 mL water, and incubated at 37°C for 30 minutes. Transposed DNA was purified using Qiagen columns. ~ 7000 cells were targeted for each sample and processed according to the 10X Genomics scATAC-seq sample preparation protocol (Chromium Single Cell ATAC Library & Gel Bead Kit, 10Å~ Genomics).