title: GSM7421659: H7, GO-APP, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: T cells were activated and expanded from PBMCs with either Beads3/28 (αCD3 and αCD28 on commercial beads) with external IL-2 (30U/ml) or GO-APP3/28 (αCD3 and αCD28 on graphene oxide antigen presenting platform) in absence of external IL-2. T cells were collected 3 days after activation. Cells were quantified using a Cell Countess II automated cell counter (Invitrogen/Thermo Fisher Scientific). A total of 10,000 cells from each experimental group were loaded on the Chromium platform (10X Genomics), and libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v3.1 and the Chromium Next GEM Chip G Single Cell Kit (10X Genomics), according to the manufacturer's instructions. Library quality was assessed using the D1000 ScreenTape on a 4200 TapeStation System (Agilent Technologies). Libraries were sequenced on an Illumina NovaSeq using the NovaSeq S4 Reagent Kit (100 cycles; Illumina).