title: GSM6738021: Hashtagged_scRNA_sample_2; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh human biospecimens were initially digested into a single cell suspension and cryopreserved in Bambanker Freezing Medium. On the day of the 10X Genomics protocol, cryopreserved samples were thawed, sorted for DAPI- CD45+ CD3+ T cells, hashtagged, stained with TotalSeq C v.1.0 cocktail from Biolegend. Library was prepared using Chromium Single Cell 5ʹ v2 protocol (10x Genomics). Briefly, cDNA amplification included 14-16 cycles and 5.8 ng-20 ng of the material was used to prepare sequencing libraries with 16 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE26/91 or PE28/91 run using the NovaSeq 6000 SP or S1 Reagent Kit. An average of 108 million reads were generated per sample.