title: FACS-seq Cbe pfl  ZTP riboswitch terminator loop, Untreated starting library, Replicate 1
design: 2-step PCR of purified plasmids from sorted and cultured cells. First round PCR (3 cycles) installs a UMI and compatibility for Illumina Truseq adapter primers in Round 2 (10 cycles). 2-step SPRI bead purification to isolate amplicon.