title: GSM7674852: Human blastocyst, extracellular vesicles 9; Homo sapiens; RNA-Seq
library_construction_protocol: Total embryonic RNA was extracted from all 24 blastocysts (12 blastocysts from control-embryos group and 12 blastocysts from EV-embryos group) using a SMART-Seq v4 ultra-low input RNA kit for sequencing (Takara Bio). RNA from each blastocyst was reversed transcribed into cDNA, amplified and purified with the same kit. cDNA libraries were constructed using the Nextera XT DNA library preparation protocol (Illumina), according to the manufacturer's protocol.