title: GSM7425646: SNU719 7.5uM DEC 2; Human gammaherpesvirus 4; Bisulfite-Seq
library_construction_protocol: DNA was extracted as follows using the DNeasy Blood & Tissue Kit (Qiagen, ID 69504) according to the manufacturer's protocol. genomic DNA was spiked with lambda phage DNA and digested with MspI restriction enzyme. Fragments were ligated with methylated adapters (NEB) to protect adapter sequences from downstream bisulfite treatment. Digested DNA was bisulfite converted using EZ DNA Methylation Kit (Zymo). All libraries were prepared using NEBNext adapters and indexed with i7 primers. These methylation- based libraries were spiked with 25% phiX Illumina library to ensure sequence diversity and sequenced 75bp paired-end on Illumina HiSeq 2500 Genomic DNA was bisulfite treated so that methylated cytosine residues were protected and unmethylated cytosines were converted to T in the final sequencing.