title: GSM7675652: FTE cells, d0, rep2 (Okseq); Homo sapiens; OTHER
library_construction_protocol: EdU pulse-labeled Okazaki fragments were extracted from frozen and lysed cell pellets, isolated by ultracentrifugation on a 5-30% sucrose gradient, and purified by biotinylation-azide Click chemistry Library was constructed with adapter ligation, PCR amplification, and purification with SPRI beads. Quality testing of DNA was performed using Qubit analysis. replication origin mapping