title: GSM7139490: 28W Spl_L004; Mus musculus; OTHER
library_construction_protocol: PD-1+CD44+CD8+ T cells from spleens of NZB/W F1 mice at 12 weeks or 28 weeks of age, and kidneys of 28 weeks of age were sorted for scRNA-seq. Single-cell RNA-seq libraries were prepared with a Chromium Single Cell 5′ v2 Reagent Kit. Using primers specific to TCR constant regions, the Single-Cell V(D)J Enrichment Kit (10× Genomics) was used to enrich full-length TCR V(D)J segments from amplified cDNA from 5' libraries.