title: GSM8145438: HEK 293 cells, siCNOT4-Nuclear RNA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Ambion).  Cytosolic and nuclear RNA extractions were performed following the protocol guidelines (Gagnon, K.T., et al., Analysis of nuclear RNA interference in human cells by subcellular fractionation and Argonaute loading. Nat Protoc, 2014. 9(9): p. 2045-60.). The RNA samples were then treated with Turbo DNase (Ambion) and extracted using Acid Phenol:Chloroform (Ambion). Ribosomal RNA depletion and library preparation of total, cytosolic and nuclear RNA were done using QIAseq FastSelect rRNA HMR and NEB ultraII Directional RNA Library Prep kits following manufacturer's protocols.