title: GSM8316797: 20042x3, WT PFC, Social 35, F; Mus musculus; RNA-Seq
library_construction_protocol: mPFC and cerebellum were microdisected from C57Bl6/J mice after isoflurane anesthesia and decapitation. The brains were removed and quickly submerged in ice cold PBS with 15 uM actinomycin-D. Nuclei were then isolated through manual homogenation with lysis buffer containing Triton-X and 15 uM actinomycin-D. Myelin was removed via sucrose gradient and nuclei resuspended in buffer containing BSA and RNAse inhibitor. Library construction was performed by the university of Utah high-throughput sequencing core following the manufacturor's instructions (single cell 3' v3.1 protocol, 10X genomics)