title: GSM7728007: ISC-Cre_ApcNull_KrasG12D_Rep2; Mus musculus; ATAC-seq
library_construction_protocol: For the primary cells, epithelium was isolated using a 5mM EDTA chelation at 4°C for 30 minutes followed by disaggregation in 3mg/mL Type 2 Collagenase in HBSS at 37°C for 30 minutes. ISCs were then isolated via flow cytometry. The organoids were disaggregated using 5mM EDTA and mechanical dissociation followed by flow cytometry for ISC isolation. The ATAC-seq libraries were constructed using the Omni-ATAC-seq protocol using the Nextera kit (Illumina).