title: GSM7720602: M_3, replicate 3, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: The tumor tissues of MC38 tumor-bearing mice were collected, and the tissue dissociation solution was used to form single-cell suspension, and CD45+ cells were sorted by magnetic beads We used GEXSCOPETM Single Cell RNA Library Kit Tissue (Singleron Biotechnologies) to barcode single cells, capture mRNA from isolated single cells, and generate cDNA libraries for scRNA-seq. Then, individual libraries were diluted to 4 ng/μl, and pooled for sequencing on a HiSeq X platform (Illumina, San Diego, USA) with 150 bp paired-end reads.