title: GSM6736130: Puroseq, Total RNA from Hela with 4sU+UVA(4sU+UVA)-high, 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from cells was collected using Trizol and purified using Zymo Research DirectZol RNA Mini Prep Kit. Total RNA from FANCI was collected by Zymo Research Oligo Clean & Concentrator Kits. Standard FLASH protocol was followed for DHX9 (Ilik et al., 2019). Libraries were prepared according to the instructions of Illumina's Stranded Total RNA with RiboZero Plus kit for total RNA-Seq and according to the previously published protocol for FLASH (Ilik et al., 2019). RiboZero RNA-Seq, FLASH library using s-oligo