title: 16S rRNA-Seq of cucumber rhizosphere soil: control group treated with sterile water
design: V3V4 hypervariable regions of bacterial 16S rRNA gene were amplified by using the specific primer pair 338F (5-ACTCCTACGGGAGGCAGCAG-3) / 806R (5-GGACTACHVGGGTWTCTAATT-3) by applying a thermocycler PCR system. Purified amplicons were pooled in equimolar amounts and paired-end sequenced (PE 2300) on an Illumina MiSeq platform (Illumina, San Diego, USA) according to standard protocols by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China).