title: Environmental/Metagenome sample from sediment metagenome (37m)
design: The DNA for metagenomic sequencing was extracted from about 5 g of the sediment samples S-2-5 and S-2-10. The qualified DNA samples were randomly fragmented into about 300 bp pieces by Covaris ultrasonic disruptor (Covaris, USA), and then subjected to end repair, A-tailing, adapter ligation, purification, PCR amplification, and other steps to complete the library preparation. After the library construction, the libraries were first quantified by Qubit 2.0, diluted to 2 ng/l, and then the insert size was checked by the Agilent 2100 Bioanalyzer system (Agilent Technologies Inc, USA). After confirming that the insert size met the expectations, the effective concentration of the libraries was accurately quantified by the q-PCR method (library effective concentration >3 nM), to ensure the library quality. After quality control, the different libraries were pooled according to the effective concentration and the target data volume and sequenced on the Illumina Hiseq X-Ten system (Illumina, San Diego, CA, USA), producing paired-end reads of 2150 bp.