title: GSM7984510: ipsc_3 Cut & Tag; Mus musculus; OTHER
library_construction_protocol: To perform CUT & Tag analysis, we utilized the Hyperactive InSitu ChIP Library Prep Kit for Illumina (Vazyme Biotech). A total of 10,000 cells were collected as samples for the experimental group, with two replicates conducted. The prepared libraries underwent PCR amplification after extraction using phenol-chloroform and ethanol precipitation. The PCR cycling conditions consisted of an initial step at 72°C for 5 minutes, followed by denaturation at 98°C for 30 seconds. This was succeeded by 14 cycles of denaturation at 98°C for 10 seconds and annealing at 60°C for 30 seconds. The amplification process was completed by performing a final extension step at a temperature of 72°C for a duration of 1 minute, followed by holding the samples at a temperature of 4°C. For the purpose of post-PCR cleanup, a volume of DNA Clean Beads (Vazyme Biotech) equal to 1.5 times the volume of the DNA was utilized. In order to guarantee a thorough and precise sequencing analysis, all libraries that were prepared underwent sequencing on the Illumina Nova Seq 6000 platform, while strictly adhering to the manufacturer's guidelines.