title: GSM8415602: EW2; Homo sapiens; OTHER
library_construction_protocol: Paraffin-embedded mice sample tissues with transplanted human Ewing sarcoma tumors were used. A selection of FFPE tissue blocks with the best DV200 value was done performing RNA extraction from several slices of the different block tissues and calculating the percentage of total RNA fragments >200 nucleotides (DV200) with Agilent TapeStation. Kit used to assess the RNA Quality: Rneasy FFPE KIT (50)-RNA extraction kit from Qiagen (Ref. 73504). Tissue slides from selected FFPE blocks were located into Visium CytAssist FFPE Spatial Gene Expression 6.5mm slides through the CytAssist equipment following the Visium CytAssist Spatial Gene Expression for FFPE – Tissue Preparation Guide. VISIUM Cytassist FFPE from 10X Genomics.