title: GSM8417701: G93A_6; Mus musculus; RNA-Seq
library_construction_protocol: After euthanasia, mice were rinsed with 70% ethanol and BAT was exposed, removed with sterile scissors, and weighted. RNA was isolated with RNeasy Lipid Tissue Mini Kit (Qiagen, cat. #74804). Briefly, BAT tissue was homogenized in 1 ml of QIAzol Lysis Reagent. Phase separation was performed with addition of 200 l of chloroform, vigorous shaking and centrifugation at 12000 ×g for 15 min at 4°C. Aqueous phase was extracted, and 1 vol of 70% ethanol was added to provide appropriate binding conditions. The sample is then applied to the RNeasy spin column and processed according to manufacturer's instructions. Total RNA was eluted in 30 µl of RNase-free water. NEGEDIA Digital mRNA-seq research grade sequencing service (Next Generation Diagnostic srl)