title: GSM7847870: HCT116 cells,  DNA_damaged2, RBM42 eCLIP; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were crosslinked with 400mJ/cm^2 UV at 254nm. Cell were lysed and sonicated with Covaris E220. RNA was cleaved by 40 units of RNase I for 3 minutes at 37°C. 3' RNA adapter were ligated to the RNA. RT performed by SuperScript III using a primer for the RNA adapter. Second adapter were ligated to the 3' end of the cDNA. Library amplify by PCR using Q5 polymerase. Library were size selected by 3% low melt agarose gel