title: GSM7504456: Mm-prostate-04; Mus musculus; RNA-Seq
library_construction_protocol: To obtain single cells, intact prostate tissues were collected from 12-week-old Srd5a2-/- and Srd5a2+/- mice. Whole fresh prostate tissues were subjected to a digestion process using collagenase type I supplemented with 10 μM rock inhibitor, 1 nmol Dihydrotestosterone, and DNase I in Hank's Balanced Salt Solution (HBSS) for 2 hours at 37°C. The cells were further treated with TrypLE Express supplemented with 10 μM rock inhibitor for 10 mins at 37°C. The resulting cell solution was neutralized in HBSS supplemented with 5% fetal bovine serum and 1% penicillin-streptomycin solution. Subsequently, the cells were resuspended in Red Blood Cell lysis buffer and incubated for 3 min on ice to remove red blood cells. The single cell suspension was washed with a 0.02% PBS-BSA solution and counted. The viability of cells was between 80%~90%, assessed by Trypan Blue.