title: Metagenomic DNA from ruminal fluid of sheep
design: DNA from rumen fluid from sheep supplemented with different doses of fibrolytic enzymes was extracted with the ZymoBIOMICS DNA miniprepkit; Bacterial 16S and Eukaryotic 18S ribosomal RNA genes targeted sequencing was performed using the Quick-16S NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial and archaeal  V3-V4 region of 16S gene, as well as 18S gene, were amplified using Zymo's specific primers. The final library was sequenced on Illumina Nextseq with a P1 reagent kit (600 cycles) with 30% PhiX spike-in.