title: GSM6701346: Circ-seq 8w biol rep 1; Mus musculus; OTHER
library_construction_protocol: Heart samples from embryonic, neonatal and adult C57BL/6J mice were digested and then subjected to alkaline treatment and column chromatography purification. The purified eccDNA samples were used as templates for amplification reactions by phi29 polymerase . The amplified DNA was then fragmented with the Bioruptor sonicator , and the library was prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®.