title: GSM7426264: MES, Injury_D7; Mus musculus; RNA-Seq
library_construction_protocol: Nerves were freshly harvested in F12 medium, pooled, finely chopped and incubated in dissociation buffer (Collegenase Type II 50ul/ml, Hyaluyronidase 1:50, DNAse 2.5μg/ml) in DMEM and incubated on rotor at 37C with intermittent mixing for 30 min. Enzymes were inactivated with 5% FBS+ 2mM EDTA in PBS w/o Ca2+, Mg2+. Myelin-removal beads were used for the intact nerve sample. Cell suspension was passed through cell strainer and resuspended in 2% BSA PBS w/o Ca2+, Mg2+ and TdTomato + cells were FACS purified.