title: GSM7422452: 8 DAP seed maternal tissues replicate 2 [89203_SMT_08_REP2]; Hordeum vulgare; RNA-Seq
library_construction_protocol: Total RNA was isolated using Rneasy(R) Plant Mini Kit (QIAGEN) and Spectrum ™ Plant Total RNA Kit (Sigma-Aldrich) according to the manufacturer's instructions including on-column DNase I (Sigma-Aldrich) treatment and stored at -80 °C until use. The RNA quality was checked using Bioanalyzer 2100 with RNA 6000 Pico Chips (all Agilent) and samples with RNA integrity number > 6.8 were processed into RNA-Seq mRNA libraries using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® with poly-A selection. The polyA RNA-enriched libraries were sequenced as single end 100-bp RNA seq reads on the HiSeq2500 instrument (Illumina) at Vienna BioCenter.