title: GSM8417093: IGROV1, minus dox, PAX8 ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked in 1% formaldehyde for 15 min and then quenched with 125 mM glycine for 5 min. Chromatin was isolated by adding lysis buffer, followed by disruption with a Dounce homogenizer. Lysates were sonicated and the DNA sheared to an average length of 300-500 bp with Active Motif's EpiShear probe sonicator (cat# 53051). Illumina sequencing libraries were prepared from the ChIP and Input DNAs on an automated system (Apollo 342, Wafergen Biosystems/Takara).