title: GSM7093760: LNCaP H3K27ac HiChIP_rep2; Homo sapiens; OTHER
library_construction_protocol: Cells were pelleted, then resuspended and fixed in 1% formaldehyde for 10 minutes. Cells were then treated with 125 mM glycine for 5 minutes to stop crosslinking. HiChIP and library construction was performed as described in Mumbach et al. Nature Methods 2016 (see supplementary protocol) with the following modifications: Ishikawa cells were digested with restriction enzyme DpnII. Crosslinked chromatin from Ishikawa cells was sonicated using an EpiShear probe-in sonicator (Active Motif) with three cycles of 30 seconds at an amplitude of 40% with 30 seconds rest between cycles. Crosslinked chromatin from T-47D and LNCaP cells was sonicated using Covaris E220 with the settings of fill level=10, duty cycle=5, PIP=140, cycles per burst=200, time=4 mins.