title: Microbe sample from Gluconobacter frateurii
design: Isolates were inoculated into 10 mL MRS or TGY broth and grown at 28 C overnight with 250 rpm shaking for DNA extraction using a Zymo Quick-DNA Fungal/Bacterial Miniprep kit. The DNA was further purified with a Zymo Clean and Concentrator-5 kit to improve the purity. Approximately 2 g were submitted for sequencing by the Texas A&M AgriLife Research: Genomics and Bioinformatics Service. Barcoded libraries were prepared using a PerkinElmer NEXTFLEX Rapid XP DNA-Seq Kit HT, pooled, and sequenced on an Illumina Miseq platform with v3 chemistry for 2 x 300 bp reads.