title: GSM7311380: XT_E2F1_MO_st7_CANTAC_rep1; Xenopus tropicalis; OTHER
library_construction_protocol: X. tropicalis embryos were collected and homogenized with a P20 pipette tip. The nuclei were captured using ConA beads, followed by washing and lysing in 50 μL of lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin) for 3 min on ice. Immediately after lysis, samples were then incubated with the Tn5 transposase and tagmentation buffer at 37°C for 30 min (Vazyme Biotech, TD501) PCR was then performed to amplify the library for 12-18 cycles using the following PCR conditions: 72°C for 3 min; 98°C for 30 s followed by thermocycling at 98°C for 15 s, 60°C for 30 s and 72°C for 40s, and finally 5 min at 72°C. After the PCR reaction, libraries were purified with 1.2 ×DNA clean beads (Vazyme Biotech, N411) CANTAC-seq (similar to ATAC-seq)