title: Metabarcoding Bacteria: SI_21
design: The 16S rRNA gene V3-V4 variable region PCR primers 341F (CCTACGGGNGGCWGCAG) / 785R (GACTACHVGGGTATCTAATCC) were used in a 30/35 cycle PCR. After amplification PCR products are pooled, purified and used to prepare an Illumina DNA library.