title: GSM7426063: WT,cDC1,d2,Spleen,2,12080; Mus musculus; RNA-Seq
library_construction_protocol: cDC1s (Lineage- XCR1+ CD11c+), red-pulp macrophages (Lineage- F4/80+ CD64+), and pDCs (Lineage- F4/80- B220+ BST2+ Ly6C+) were sorted from the spleens of uninfected wildtype mice, or those that were d1 or d2 p.i with MCMV. where Lineage refers to CD3, TCRb, CD19, NK1.1, Ly6G. In each independent experiment, RNA was isolated from sorted cells using the Arcturus PicoPure RNA Isolation Kit (ThermoFisher) in accordance with the manufacturer instructions. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 0.7-2 ng total RNA with RNA integrity numbers ranging from 9.2 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 9-10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a PE100 run, using the NovaSeq 6000 S1 or S4 Reagent Kit (100 or 200 cycles) (Illumina).