title: GSM7211275: WT.Liver; Mus musculus; RNA-Seq
library_construction_protocol: CD8 and CD4 T cells were enumerated and mixed at 1:1 ratio. Same numbers of cells from different mice of the same group were pooled, generating 4 samples for scRNA-seq library preparation: WT spleen, WT liver, Id3 spleen, Id3 liver. scRNA-seq libraries were generated using Chromium™ Single Cell 3' GEM, Library & Gel Bead Kit v3 following the manufacturer's manual (10X Genomics). 10,000 cells from each sample were loaded onto the Chromium Chip B to generate single-cell gel beads-in-emulsion (GEM) for a target recovery of 6,000 single cells. mRNA from single cells were reverse transcribed, barcoded and amplified via PCR for library construction.