title: GSM8189544: A02287-NSC-0.6 µM TG; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from both control and ER stress-challenged NSCs was extracted using a commercially available RNeasy Mini Kit (Qiagen, Germantown, MD, USA) and by following the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and an Agilent 2200 TapeStation system (Agilent, Santa Clara, CA, USA). 1 µg of high-quality total RNA per sample and the reagents supplied in Illumina Stranded mRNA sample preparation kit v2 (Illumina, Inc., San Diego, CA, USA) were used to prepare mRNA sequencing libraries.