title: GSM8241999: PKM2_KD, H3T11ph_CUT&Tag; Homo sapiens; OTHER
library_construction_protocol: Cells were mixed with NovoNGS® Concanavalin A-coated Magnetic Beads (N251). Resuspend cells in ice-cold Antibody buffer and add 0.5-1 µL primary antibody to each sample. Mix the secondary antibody 1:100 in Dig-wash buffer while gently vortexing to allow the solution to dislodge the beads from the sides. Mix pA-Tn5 adapter complex in Dig-300 buffer to a final concentration of 1:250 for 100 µL per sample.Squirt in 100 µL of the pA-Tn5 mix while gently vortexing to allow the solution to dislodge most or all of the beads. After a quick spin, place the tubes on a magnet stand to clear and pull off the liquid. Add 300 µL Tagmentation buffer. DNA extraction. The remainder of the CUT&Tag library preparation was performed as described TruePrep DNA Library Prep Kit V2 for Illumina (TD503). The qualified libraries were sequenced on Illumina Nova Seq 6000.