title: V3V4 region on 16S rDNA gene amplicon from envrionmental DNA extracted from anaerobic digestion sludge
design: DNA was isolated using Fast DNA Spin Kit for Feces (MP Biomedicals, Illkirch, France). V3V4 hypervariable region of 16S rDNA gene was amplified using Bakt_341F/Bakt_805R primer pair (Muyzer et al., 1993; Mizrahi-Man et al., 2013).