title: GSM7311138: border zone and injured ventricle tissues, mut; Danio rerio; RNA-Seq
library_construction_protocol: To isolate cells of wild-type and cd74a; cd74b mutant samples, the border zone and injured tissues of four ventricles (from two females and two males each) were used.  For both EdCs and whole border zones and injured tissues, dissociation was performed using the Pierce Primary Cardiomyocyte Isolation Kit, (Thermo Fisher Scientific) according to the manufacturer's protocol and with a few modifications, as previously published (PMID: 34516874). Dead cells were removed using a LeviCell 1.0 (Levitas Bio), following tissue dissociation. The cell suspensions were counted with Moxi cell counter and diluted according to manufacturer's protocol to obtain 10.000 single cell data points per sample. Each sample was run separately on a lane in Chromium controller with Chromium Next GEM Single Cell 3? Reagent Kits v3.1 (10xGenomics). Single cell RNAseq library preparation was done using standard protocol. Sequencing was done on Nextseq2000.