title: RNA-seq of ovarian cancer PDX-derived cells
design: Ascites-derived cells were maintained in PBS until cell count was done and 1 million pellets were kept at -80C until RNA extraction was performed. Then RNA was harvested using Trizol protocol. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB)