title: GSM6697884: slc7a5 fl/fl, rep1; Mus musculus; RNA-Seq
library_construction_protocol: 4 8-weeks-old mice were used for isolation of intestinal epithelial cells, the intestine was cut into 2-5 mm segments and incubated in phosphate-buffered saline (PBS) containing 20 mM EDTA for 30 min on ice on a rocker. Epithelial cells were released by vigorous shaking or pipetting and passed through a 70 um strainer to collect enriched crypts,  which were then washed with cold PBS containing 0.1% bovine serum albumin (BSA). The enriched intestinal crypts as isolated above were washed twice in PBS, centrifuged at 300 g for 3 min, and the epithelial cells were dissociated with TrypLE express (Invitrogen) for 1 min at 37 °C. The dissociated single cells were stained with an EpCAM (Thermo Fisher Scientific), CD45(Thermo Fisher Scientific), and propidium iodide (Thermo Fisher Scientific). The EpCAM+ CD45- live cells were sorted by flow cytometry and cell number was counted. The estimated ideal number of cells were used for scRNA-seq according to the manufacturer's instructions (10x Genomics kit, chromium single cell 3' reagent kits V2).