title: GSM7307105: HC,rep8,scRNA_seq; Homo sapiens; RNA-Seq
library_construction_protocol: Biopsy specimens were collected into RPMI 1640 medium on ice and processed immediately into single cells. Single cells were dissociated from the intestinal samples obtained by biopsies through the method mentioned in the FACS analysis. The single cells were loaded into Chromium microfluidic chips with v3 chemistry, then barcoded with a 10× Chromium Controller (10X Genomics, Pleasanton, CA, USA). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries were constructed with reagents from a Chromium Single Cell v3 reagent kit (10X Genomics) according to the manufacturer's instructions.