title: GSM6433804: Male_WT_Rep2; Anopheles gambiae; ATAC-seq
library_construction_protocol: The experiment was performed with flash frozen tissues, which were homogenized in cold PBS and passed through a cell strainer. The cell suspension was counted and 50,000 cells were used for each reaction. The libraries were generated according to the following protocol: 10.1002/0471142727.mb2129s109. We used Tn5 prepared by the IMB protein production core facilty. ATAC-seq libraries were amplified using NEBNext Ultra II Q5 Master Mix with standard protocols and 15 PCR cycles. Libraries were cleaned up using 1.3 volume Ampure XP beads and pooled together for paired-end sequencing.