title: GSM7311372: XT_st8_CANTAC_rep1; Xenopus tropicalis; OTHER
library_construction_protocol: X. tropicalis embryos were collected and homogenized with a P20 pipette tip. The nuclei were captured using ConA beads, followed by washing and lysing in 50 μL of lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% NP-40, 0.1% Tween-20, and 0.01% digitonin) for 3 min on ice. Immediately after lysis, samples were then incubated with the Tn5 transposase and tagmentation buffer at 37°