title: VC-water 6
design: The bacterial DNA was extracted from overnight culture using Qiagen DNA extraction kit (Qiagen, India) according to the manufacturers instructions. The quality of the DNA was checked using a nanodrop (Thermo Scientific, USA). A ratio of OD 260/280 value of 1.8-1.9 is taken for sequencing.The total DNA was sequenced using Illumina HiSeq2500 sequencing platform and the next generation sequencing library preparation were constructed following manufacturers instruction. (NEBNext UltraII DNA Library Prep Kit for Illumina, Illumina, San Diego, CA, USA). Sequencing was carried out using a 2 X 150 paired end configuration, image analysis and base calling were conducted by HiSeq Control Software (HCS)+OLB+GA pipeline-1.6 (Illumina) on the HiSeq Instrument HiSeq2500.