title: GSM6514207: SF_1630; Mus musculus; RNA-Seq
library_construction_protocol: RNA from BM LSKs of Scl-CreERT:Flt3ITD/ITD C57BL/6J and controls was isolated using Picopure kit. cDNA libraries were prepared using the NEBNext Ultra RNA Library Prep kit with the polyA selection module (New England Biolabs) and purified with AMPure beads (Agilent). Libraries were quantified with the KAPA Library Quantification Kit for Illumina (Kapa Biosystems) and pooled. Sequencing was performed on the Illumina NextSeq 500/550 Sequencer High Output mode (1 × 75 bp).