title: FACS-seq Cbe pfl ZTP riboswitch terminator loop, 0.1 mM Z, Bin 1, Replicate 2
design: 2-step PCR of purified plasmids from sorted and cultured cells. First round PCR (3 cycles) installs a UMI and compatibility for Illumina Truseq adapter primers in Round 2 (10 cycles). 2-step SPRI bead purification to isolate amplicon.