title: WES of SKMEL2: WT isolate 2
design: The genomic DNA was randomly sheared into short fragments with the size of 180-280 bp. The obtained fragments were end repaired, A-tailed, and further ligated with Illumina adapters. The fragments with adapters were PCR amplified, size selected, and purified. Hybridization capture of libraries was proceeded according to the following procedures. Briefly, the prepped libraries were hybridized in the buffer with biotin-labeled probes, and magnetic beads with streptavidin were used to capture the exons of genes. Subsequently, non-hybridized fragments were washed out and probes were digested. The captured libraries were enriched by PCR amplification. The libraries were checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina Novaseq 6000 platforms with PE150 strategy, according to the effective library concentration and data amount required.