title: GSM8241231: 181.SNP.4h; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using RNAeasy Micro Kit (Qiagen) following manufacturer's instructions. Libraries were prepared using the Illumina Stranded Total RNA Prep kit starting from 7.5 ng of total RNA and using 14 PCR cycles for library amplification. The kit for the index sequences used was IDT Illumina RNA UDI A Lig 96 Indexes 96 Spl. RNA sequencing was  performed on one S4 lane on an Illumina NovaSeq 6000 sequencer, using NovaSeq 6000 S4 v1.5 Reagent Kit (300 cycles), and a paired-end 2 x 150 pb strategy. Between 20 and 44 million paired-end raw reads were produced per library.