title: GSM8059345: Sample_UIC7_5_310_267; Xenopus tropicalis; RNA-Seq
library_construction_protocol: 10 ml of an ERCC RNA Spike-In Mix (Thermo Fisher Scientific) was added to 860 ml of DEPC-treated water and then diluted 1:10 into Trizol. 10 embryos per timepoint and condition (UIC and MO) were homogenized in 100 ml of Trizol+Spike-In mixture. Each sample was frozen using ethanol and dry ice and then stored at -80 °C. All samples were processed for RNA extraction at the same time. Following isopropanol precipitation, RNA pellets were resuspended in DEPC-treated water and precipitated with 5M LiCl at 4 °C overnight. This solution was centrifuged at 4 °C, and RNA pellets were washed twice with 70% ethanol. Final RNA products were resuspended in DEPC-treated water (2 ml/embryo). RNA libraries were prepared for Illumina sequencing using standard polyA+ and RiboZero Illumina protocols