title: NanoRanger 2 of 14DG1602
design: The native DNA and the products thereafter were quantified using Qubit. Sample products were barcoded and library-prepared using the ONT Ligation Sequencing Kit (SQK-LSK109) and Native Barcoding Expansion 1-12 Kit (EXP-NDB104) following the manufacturers instructions (available from https://community.nanoporetech.com/protocols), expect that all elutions were done for 10 min at 37C. Shearing step was not performed due to the already appropriate fragment size. Approximately 1.2 ug of genomic DNA was used to make sequencing libraries. Libraries were aliquoted and loaded onto a Release 9.4.1 flow cell for sequencing on an ONT GridION sequencer. Sequencing experiments were run for up to 72 h. DNA libraries were loaded onto the same flow cell after washing approximately every 24 h to increase output.