title: GSM8009820: NK, WT, Hi-C, rep1; Mus musculus; Hi-C
library_construction_protocol: For ChIP-seq, cells were fixed with 1% formaldehyde for 10 minutes and crosslinked chromatin was sheared by sonication to obtain 150-500 base-pair fragments. For ATAC-seq, cells were lysed to isolate the nuclei and the isolated nuclei were then incubated with Tn5 transposase and tagmentation buffer. For Hi-C, cells were cross-linked, lysed, and nuclei were digested with DpnII. For RNA-seq, total RNA was harvested using Trizol reagent. Nucleic acid libraries were prepared using illumina Novaseq 6000.