title: mRNA from ICM of blastocysts
design: Each sample was prepared using three inner cell masses (ICMs) from blastocysts, with two replicates per group. The samples were washed three times with PBS-PVA to avoid potential contamination. Then, we used the Phusion Hot Start II High-Fidelity PCR Master Mix (F-565S, Thermo Fisher) to prepare the libraries according to the manufacturers instructions provided with the NEBNext Ultra II DNA Library Prep Kit (E7805S, New England Biolabs, MA, USA). The libraries were sequenced on the NovaSeq 6000 platform (Illumina, CA, USA)