title: GSM7675690: Donor 1 (s1), Mock, 72h; Homo sapiens; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted with the Extract-All reagent (Eurobio, #GEXEXT040U) following the manufacturer's protocol. Extracted RNA was treated for 1 h at 37 °C with RNAse-free DNase I (Invitrogen, #AM2222), which was subsequently inactivated at 70 °C for 20 min. RNA reverse transcription was performed using the Superscript® IV RT kit (Invitrogen, #18090010), according to manufacturer's instructions. mRNA libraries were prepared using the TruSeq® Stranded RNA HT (w/Ribo-Zero™) kit to increases the depth of sequencing. Each library was pair-end and was sequenced using 75x2 cycles on a NextSeq 500 Illumina platform.