title: Illumina HiSeq 2000 sequencing; Histone H3, lysine 27 acetylation ChIP-seq in NALM6 expressing ETV6-RUNX1
design: Histone H3, lysine 27 acetylation ChIP-seq in NALM6 expressing ETV6-RUNX1
library_construction_protocol: Cells were sorted to high purity for transgene expression (GFP), expanded in vitro, and approximately 10^8 cells harvested for subsequent ChIP experiments.  Cells were crosslinked with 1% formaldehyde and sonicated to yield chromatin of 100â€“500 bp. ChIP was performed by as previously described (May et al., 2013) using antibodies: H3K27ac. See May et al, 2013. https://doi.org/10.1016/j.stem.2013.09.003