title: GSM8316666: Liver, CDAA-HFD 12 weeks, Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Liver tissue were collected and snap-frozen in liquid nitrogen. RNA was isolated using the NucleoSpin® kit (MACHEREY-NAGEL). RNA sequence libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced on NextSeq 550 (Illumina) with NextSeq 500/550 High Output Kit V2 (#75 CYS, Illumina)