title: GSM7984616: EtOH-2b-ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq samples were prepared following the protocol described in (Orlando, Douglas et al. 2020) with minor modifications. First 50K nuclei were isolated in technical duplicate per sample. DNA was tagmented for 30 min at 37C using the Illumina Tagment DNA kit (20034211). Tagmented DNA was cleaned up using the MinElute PCR Purification Kit (Qiagen). Extracted DNA was subjected to PCR amplification using unique primers sets (Nextera XT v2 Full set (N7-S5)). The number of PCR cycles used for library amplification was determined by qPCR. Finally, a double-sided size selection was performed using AMPure XP Beads (Beckman Coulter). Libraries quality was assessed using by Tapestation and the technical duplicate of each sample with the most pronounced nucleosome banding was sequenced.