title: RNAseqEsophagusBE_Patient3 (A14)
design: RNA was isolated using TRI reagent (TRIzol Reagent, Sigma, Catalog #T9424, St. Louis, Missouri, USA) following the manufacturers guidelines. Total RNA yield was measured using NanoDrop One (Thermo Fisher Scientific Inc.) and 1ug of total RNA (RIN>6) was sent for bulk RNA sequencing. RNA sequencing was preformed at the University of Nebraska Medical Center following the Universal Plus mRNA-Seq with NuQuant (Tecan Genomics) protocol.