title: DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - III
design: DEAD-box ATPase Dbp2 mediates mRNA release after 3'-end formation - III
library_construction_protocol: Cells were grown in EMMG, then shifted to YES for 5h or 9h. S. cerevisiae spike-in cells were added at at 1:5 OD600 ratio immediately before harvesting by centrifugation. Total RNA was extracted with the hot phenol method.  Libraries were prepared with the VAHTS Stranded mRNA-seq Library Prep Kit (Vazyme Biotech) after poly(A) selection.