title: GSM8241855: Het_Th2_R2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from 106 cells using RNeasy mini kit (Qiagen) with a separate step for DNAse I digestion (Qiagen). ERCC Spike-in control mix-1 (Life technologies) was added to each sample prior to processing (1μL of a 1:10 dilution of mix-1). The rRNA depletion and library construction was processed with the ScriptSeq Complete Gold Kit (Epicentre) according to the manufacturer's instructions. The index for individual samples was added in order to multiplex 9 samples in each sequencing lane. The quality of libraries was assessed using a DNA1000 chip on the Bioanalyzer (Agilent) and the concentration was measured using the Qubit DNA assay. Sequencing was performed on the Illumina platform with HiSeq 2000 paired-end 100 bp sequence type.