title: GSM7785470: HP4HA2_replicate_2; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed gently and small molecular weight DNA, consisting mostly of excision products separated from chromosomal high molecular weight DNA by centrifugation. Briefly, the excision products were captured by TFIIH/XPG IP and treated with carbonate-bicarbonate buffer (pH 9.6) to convert the AFB1-N7-dG to the ring-opened AFB1-FAPY-dG adducts. Following adaptor ligation, the adaptor-ligated excision products were further purified by IP with anti-aflatoxin monoclonal antibody. Then sulfolobus DNA polymerase IV was used to bypass the AFB1-FAPY-dG adduct during the primer extension step of tXR-seq. After purification of the primer extension products, Kapa Hotstart Readymix was used for amplifying the library.