title: GSM8417595: HIE_7d, scRNA-seq; Rattus norvegicus; RNA-Seq
library_construction_protocol: A total of 18 rats (3 rats per group) were anaesthetized and transcardially perfused with ice-cold saline. Injured or normal brain were immediately extracted and maintained in an ice-cold Hibernate-A medium (Gibco). Single-cell suspensions were obtained by using a Neural Tissue Dissociation Kit (P) (Miltenyi Biotec) according to the manufacturer's protocol. The suspensions were then strained using a cell strainer (70 μm) and centrifuged. Cell pellets were resuspended in 33% Percoll (GE Healthcare) layered on 70% Percoll and centrifuged for 15 min at 1800 r.p.m. Cells were obtained from the 30–70% interface and washed with Hank's Balanced Salt Solution twice. All steps were conducted at 4 °C except digestion. libraries were prepared with Chromium Single cell 3' Reagent v3 Kits according to the manufacturer's protocol.