title: GSM8146169: CASZ1_replicate 1; Homo sapiens; OTHER
library_construction_protocol: Cells were then dissociated by warm 0.25% Trypsin for 10 min at 37°C. Cells were then centrifuged at 200 g for 4 min at 4°C, washed twice with 1x PBS. Nuclei were isolated according the manufacture's protocol and processed using the CUTANA CUT&RUN assay (Epichypher). CUT&RUN libraries were prepared using the CUTANA CUT&RUN Library Prep Kit (Epicypher).  Post amplification libraries were size selected at 200-700bp in length using SPRIselect beads. The concentration and quality of the libraries were determined using Qubit (Invitrogen) and Bioanalyzer (Agilent). Constructed  libraries were sequenced on the Illumina NovaSeq X  platform, obtaining 150-nucleotide paired-end reads.