title: Nanopore sequencing of synthesized cDNA:dU incorporated
design: RNA was isolated from HeLa cells via Quick-RNA kit (Zymo).  First-strand synthesis was performed using a superscript III kit (Invitrogen) as per the manufacturer's protocol, except with either 1uM dUTP or an addition 1uM dTTP added to the reaction mixture.  Synthesized cDNA was prepared for sequencing using a Rapid Barcoding Kit (Oxford Nanopore) and sequenced on an R9.4.1 flow cell on a minION mk1C device.