title: GSM7847270: E7.0, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Embryos staged at E7.0 were collected and washed with cooled 0.5% BSA/PBS for twice. Sufficient embryos were pooled and then subjected to 200 uL TrypLE for cell dissociation for 10 min at 37°C with frequent gentle mixture. Single-cell suspension of embryos were then quenched and washed with 0.5% BSA/PBS, and finally filtered using 40 μm Flowmi cell strainer. The acquired single cell suspension were then subjected to nuclei isolation, library preparation by following the manufacturer's instruction. The library was sequenced on Novaseq 6000 platform with recommended sequencing depths and read lengths. scMulti-omics