title: GSM6460381: UM-SCC-104_baitE6E7_replication1; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed and cross-linked DNA was digested with the primary restriction enzyme. Next, digested DNA was subjected to proximity ligation using T4 DNA ligase (EL0013, Thermo Scientific), followed by cross-link removal using Proteinase K (P8107S, New England Biolabs), which resulted in 3C libraries. The 3C libraries were then subjected to a second restriction enzyme digestion, followed by a circularization reaction using T4 DNA ligase. The 4C template was used to perform a scaled-up inverse nested PCR. Inverse primers were designed and appropriate adaptors and index sequences were then added to the inverse primer pair.