title: GSM6638320: ∆Snf-1 biol rep1; Yarrowia lipolytica; RNA-Seq
library_construction_protocol: The cell pellets in Isogen (Nippo gene) were lysed by glass bead shocker. The RNA was extracted from the yeast cell lysates by chloroform extraction, followed by the removal of genomic DNA using an RNase-free DNase I Set (Omega biotek). The quality of extracted RNA was checked by a Nanodrop photometer (Thermo Fisher). High quality of RNA with the ratio for A260/280 (> 2.0) and A260/230 (> 2.0) was then proceeded to an RNA library preparation for RNA sequencing. RNA libraries were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (Cat No. 7420)