title: RNA-Seq of Mus musculus: control stool 4
design: DNA from feces was extracted with a Powerfecal Pro DNA kit (Qiagen). The V4 hypervariable region of the 16S rRNA gene was PCR-amplified using the 515-F and 806-R primer pair with Illumina adapters and a unique 12-bp barcode for each sample. DNA products were then pooled in equimolar concentration, products longer than 200bp were selected using a QIAseq beads, and sequenced on a 2x150 bp Illumina MiSeq platform (Illumina) at the PANDA core for Genomics and Microbiome research, University of Arizona, USA