title: Genome-seq XDR- E. coli
design: evolved XDR-E. coli M19736 at day 1 of subculture was extracted using the mini kit QIAamp DNA (QIAGEN) following the manufacturers protocol for Gram-negative bacteria. Concentration and purity control were performed by measuring on a NanoDrop instrument (Nano500 Micro-Spectrophotometer, Allsheng). DNA was sequenced on a MiSeq sequencer (Illumina- pair ends). The sequencing was performed in the Genomics and Bioinformatics Unit of ANLIS Malbrn (Argentina) and the library preparation was made according to the manufacturer's protocol