title: GSM8190259: Tumor_24, TGFb_vacc_IL6R_block; Mus musculus; RNA-Seq
library_construction_protocol: Tumor fragments (≤ 20 mg) in RNAlater (Invitrogen) were transferred to RLT buffer (Qiagen) and mechanically homogenized with a Tissue Lyser (Qiagen). RNA was extracted with the RNEasy Plus Mini Kit (Qiagen), RNA with RIN > 7 was used. mRNA was enriched using using Oligo dT beads (NEBNext). The mRNA was fragmented, random-primed for cDNA synthesis (NEBNext). Hairpin adapotors were ligated to end repaired cDNA and PCR was used to add index (NEBNext). Library was size selected using AMPure beads and quantified using KAPA (Roche).