title: GSM7674873: eIF4G2 overexpression total bio rep 2; Homo sapiens; RIP-Seq
library_construction_protocol: For RNA isolation, TRIzol reagent was added directly to the tube containing beads resuspended with RIP lysis buffer, followed by the conventional precipitation method. The RNA pellet was then treated with DNase I and EDTA to remove any DNA contamination, before being repurified using phenol/chloroform/isoamyl alcohol (25:24:1, pH 6.7) to obtain pure RNA samples. Sequencing libraries were constructed using the NEBNext® UltraTM II RNA Library Prep Set following the manufacturer's instructions.