title: Human Fecal Gut Microbiota 16S rRNA Sequencing
design: Fresh feces were collected from the participants using sterile stool tubes. After collection, the samples were delivered to the laboratory in an insulated box and frozen in a -80C refrigerator. DNA was extracted from the collected samples using a DNA extraction Kit, and 16S V3-V4 region of the ribosomal RNA was amplified by PCR. The amplified 199S rRNA gene fragment was constructed into a gene bank. The constructed gene bank was subjected to high-throughput sequencing using Illumina sequencing platforms. Subsequently, the data obtained from sequencing were processed for quality control and removal of low-quality sequences. RDP classifier was used to annotate each sequence for species classification.