title: GSM7425891: male, WT, no-running control, tricpes [6795]; Mus musculus; RNA-Seq
library_construction_protocol: Triceps were homogenized under liquid nitrogen by mortar and pestle. The fine powder was transferred to a 1.5 mL Eppendorf tube with a spatula and mixed with 1 ml trizol reagent for RNA extraction. RNA was separated with chloroform, precipitated with isopropanol, and washed with 75% ethanol. Genomic DNA was removed and RNA was purified using the Qiagen RNeasy Plus Mini Kit (Qiagen, Hilden, Germany). Purified RNA was submitted to Lexogen Gmbh (Vienna, Austria) for 3' mRNA sequencing. Sequencing-ready libraries were produced using a QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (015UG009V0252) using 50 ng RNA per sample. Sequencing was performed on an Illumina NextSeq 500 sequencer with a v2 SR75 High Output kit at Lexogen GmbH.