title: GSM8416995: GSC3565 cells, Chaetocin_treated_2; Homo sapiens; ATAC-seq
library_construction_protocol: Nuclei were collected by centrifugation and resuspended in the transposition reaction mix containing Tn5 transposase (Illumina, cat# FC-121-1030) and incubated at 37°C for 30 mins. The transposed DNA was purified using the DNA Clean & Concentrator-5 (Zymo, cat# D4003) and PCR amplified using Nextera PCR primers. The amplified libraries were purified with the KAPA HiFi HotStart ReadyMix (Roche, cat#KK2602) and quantified using a Qubit fluorometer (Thermo Fisher Scientific). Libraries were sequenced on an Illumina platform to generate paired-end reads. ATAC-seq libraries were prepared following the Omni-ATAC protocol. Briefly, GSCs were washed with cold PBS and lysed in ATAC lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal CA-630).