title: GSM7673168: embryo_rep6; Bos taurus; RNA-Seq
library_construction_protocol: Polyadenylated RNAs were captured and reverse transcribed with an Oligo(dT) primer In brief, single cells were incubated at 72 °C for 3 min with lysis mixture. Reverse transcription and PCR pre-amplification were carried out with SuperScript II (Invitrogen) and KAPA HiFi HotStart ReadyMix (KAPA Biosystems). After PCR amplification , the samples with different barcodes were mixed together and then purified with DNA Clean&Concentrator-5 (Zymo Research). The cDNAs of single cells were added with biotinylated index and purified using beads (Vazyme). The cDNAs were sheared into approximately 300 bp fragments using sonication (Covaries). The fragmented cDNAs were enriche by Dynabeads ® MyOne TM Streptavidin C1 (Invitrogen). Adaptor ligation and final library were conducted by NEBNext Library Quant Kit (New England Biolabs).