title: GSM8099056: BSF,biol rep 1,ATAC_seq; Mus musculus; ATAC-seq
library_construction_protocol: Mice were treated with Control, BSF, DSF and LSF for 2 weeks, and jejunal epithelial cells were collected by flow sorting. The nuclei were collected by adding lysis solution [10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween 20, 0.01% Digitonin] on ice for 5 min and centrifuged at 500 g for 5 min. Fragmentation was performed according to the recommended conditions of the kit (Novoprotein Chromation Profile Kit for Illumina, #N245). PCR enrichment was applied using the kit (Novoprotein NovoNGS Index Kit for Illumina, #N239) with the PCR amplification cycle number set to 11. PCR products were purified according to the recommended procedure of the kit (Novoprotein DNA Clean Beads, #N240), and library quality was assessed with Qubit. The clustering of the index-coded samples was performed on a cBot Cluster Generation System used the TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and 150-bp paired-end reads were generated. ATAC sequencing was provided by Tianjin Novogene Bioinformatic Technology Co., Ltd.