title: GSM7673608: Chassis strain, without IPTG, replicate 1; Bacillus subtilis subsp. subtilis str. 168; RNA-Seq
library_construction_protocol: Total RNA was isolated by acid-phenol extraction protocol consisting of two extractions with an equal volume of acid phenol/chloroform/isoamyl alcohol (25:24:1, [pH 4.5]) followed by one extraction with chloroform/isoamyl alcohol (24:1). After adding 1/10 volume of 3 M sodium acetate (pH 5.2), RNA was precipitated with isopropanol, washed with 70% ethanol and dissolved in 100 μl of RNase free water. For transcriptome analysis, 35 μg RNA were DNase-treated using the RNase-Free DNase Set (Qiagen) and purified using the RNA Clean-Up and Concentration Micro Kit (Norgen). Library construction -including ribosomal RNA depletion- and sequencing was performed by Fasteris (Plan-les-Ouates  Switzerland). RNA depletion was performed with the Lexogen RiboCop META kit, library preparation used the Illumina TruSeq stranded mRNA kit, and the sequencing was paired-end (2 × 50 bp on Illumina NovaSeq 6000).