title: environmental DNA metabarcoding of rivers in Newfoundland and Labrador: water samples
design: Three mitochondrial DNA markers covering two genetic regions were amplified from each sample: the 12S Teleo marker (Valentini et al. 2016), the 12S MIFISH-U marker (Miya et al. 2015), and the CO1 miniFISH-E marker (Shokralla et al. 2015). Negative controls were added during extraction and PCR to screen for contamination. Samples were randomized from all three sampling years and sequenced over four sequencing runs on an Illumina NovaSeq 6000 using SP and S1 300 cycle kits with a target depth of 2,000,000 sequences/sample/marker.