title: GSM8285182: YY1_Cut&Run_LPKO_Vector_LCL_Rep1; Homo sapiens; OTHER
library_construction_protocol: Cut&Run was performed following kit protocol (Epicypher 14-1048) with a 1 min, 1% formaldehyde fixation Libraries were prepared using the CUTANA CUT&RUN Library prep kit (Epicyhper 14-1001) according to protocol instructions with modifications. End repeat was modified to 20C for 20 minutes followed by 50C for 1 hour. PCR clean up beads (CNGS-0001) were used at 1.25x after ligation and 1.1x after indexing PCR to enrich for smaller fragments.