title: GSM6701306: Mock EM-seq2; Arabidopsis thaliana; OTHER
library_construction_protocol: DNAeasy plant mini kit according to manufacturer instructions (Qiagen) 100 ng of DNA was used to create libraries with the NEBNext Enzymatic Methyl-Seq kit for Illumina. Fragmentation of the material was performed on the Covaris S2 instrument with fragmentation effciency being checked on the Agilent Bioanalyzer using the DNA High Sensitivity kit. Library preparation was perfromed as according to manufacturers recommendations including the use of methylated pUC 19 and unmethylated Lambda DNA as spike in. Library quality was checked on the Bioanalyzer using the DNA 1000 Assay and quantified with the Qubit using the High Sensitivity assay. Libraries were pooled and sequencing performed on the Illumina NextSeq 550 with 150 cycle Mid Output chemistry in 75 paired end modus. enzymatic methlylation (EM) sequencing