title: GSM6522685: TSC, RNAseq, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Plus Mini Kit (74136) following the manufacturer's instructions. mRNA was enriched from total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490). RNA-seq libraries were generated with 300 ng of total RNA using NEBNext Ultra II RNA Library Prep with (E7775) and NEBNext Multiplex Oligos for Illumina (E7600S) following the manufacturer's protocol.