title: GSM8316101: imDCs,Nano_3; Mus musculus; RNA-Seq
library_construction_protocol: ImDCs cultured on tissue culture plates（Control）and the surfaces of HA dishes (Nano and Meso) for 24 h were taken into the grinding and crushing tube with corresponding number. Add 600μL Buffer RLT Plus, homogenize the grinding machine, and let it stand at room temperature for 5 min; At room temperature, centrifuge at 12,000×g for 3 min; All supernatants were transferred to AllPrep DNA spin column; Centrifuge at room temperature of 10,000×g for 30 s, and the liquid in the collection tube was purified and collected for RNA The DNBs obtained were added to the mesh holes on the chip using high-density DNA nanochip technology, and sequenced by combined probe anchoring polymerization (cPAS)