title: GSM8242030: HeLa, GammaRay, Rep1, IP, MeRIP-seq; Homo sapiens; RIP-Seq
library_construction_protocol: polyA RNA For meRIP-seq, the libraries were constructed by Truseq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer's instructions and quantified by BioAnalyzer High Sensitivity DNA chip, and then deeply sequenced on the Illumina HiSeq X10 to generate 150-bp paired-end reads. For RNA-seq library, mRNA enrichment, cDNA synthesis, adaptor addition, circularization, PCR amplification and library examination were performed on the BGIseq 500 at Beijing Genome Institute (BGI; Shenzhen, China) For meRIP-seq, the libraries were constructed by Truseq Stranded mRNA Sample Prep Kit (Illumina) according to the manufacturer's instructions and quantified by BioAnalyzer High Sensitivity DNA chip, and then deeply sequenced on the Illumina HiSeq X10 to generate 150-bp paired-end reads. For RNA-seq library, mRNA enrichment, cDNA synthesis, adaptor addition, circularization, PCR amplification and library examination were performed on the BGIseq 500 at Beijing Genome Institute (BGI; Shenzhen, China)