title: GSM7847822: somatotrope cell line GH3, input, rep1; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Cells were fixed in 1% formaldehyde for 10 minutes, quenched with glycine, and e pellet was then washed twice in 1X PBS and 0.5% Igepal, then once more but with added protease inhibitors. The cells were pelleted one final time, supernatant removed, and pellet flash frozen and sent to Active Motif. 50 micrograms of chromatin was prepared using the ChIP-IT High Sensitivity kit