title: GSM8425414: 94_Epith; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA were then isolated by TRIzol method and further purified on collumns. A total of 1000 ng of intact total RNA was subjected to mRNA enrichment, heat fragmentation (94 ◦C, 5 min), cDNA synthesis and ligation of KAPA Unique Dual-Indexed Adapters (Roche). Ten cycles of library amplification were applied. The size distribution of the final libraries was validated using an Agilent TapeStation 2200 and High Sensitivity D1000 Reagents (Agilent) and the concentration was determined by qPCR using a Kapa Library Quantifica-tion Kit (Roche).