title: Nanopore sequencing of BrdU-labeled glioma culture:DMSO treatment
design: BT142 glioma cell lines were maintained as stem cells in stem cell media (Neurobasal supplemented with B27, N2, FGF, and EGF) or differentiated in DMEM with 10% FBS, for one week prior to coculture.  Stem and non-stem populations were co-cultured at a 1:3 ratio (stem:nonstem) in stem cell media with 2% FBS added.  24 hours after plating, 10uM dasatinib or DMSO was added to the media.  24 hours after drug/DMSO addition, 100uM BrdU was added to the media.  Cells were harvested 24 hours post-BrdU addition.  DNA was collected via Quick-DNA kit (Zymo) and prepared for Nanopore sequencing using a Rapid Barcoding Kit (Oxford Nanopore).  Library was sequenced for 24h on an R9.4.1 flow cell on a minION mk1C device.