title: GSM6840725: KO-1; Mus musculus; RNA-Seq
library_construction_protocol: Two days after the immunization, donor B cells were magnetically enriched from pooled spleens of recipient mice using APC conjugated anti-CD45.1 (eBiosciences: Cat# 17-0453-82) and anti-APC MicroBeads (Miltenyi Biotec), with the MACS system (Miltenyi Biotec). After enrichment, cells were further stained with the respective Abs and CD45.1+ B220+ CD138- FSChi cells were sorted by using FACSAria III (BD Biosciences). Total RNA isolation was achieved using RNeasy Plus Micro Kit (QIAGEN). cDNA libraries were prepared with TaKaRa SmartSeq Stranded kit (Takara) following manufacturer's protocols.