title: GSM7426366: Pool of 3 brain lesions from 482N1; Mus musculus; RNA-Seq
library_construction_protocol: Resected brain lesions were processed with the Brain Tumor Dissociation Kit (Miltenyi, ref. 130-095-942) using gentleMACS C Tubes (Miltenyi, ref. 130-093-237) and the gentleMACS™ Octo Dissociator (Miltenyi, ref.  130-096-427). For myelin removal and red cells lysis, reagents from the Adult Brain Dissociation Kit (Miltenyi, ref. 130-107-677) were used. To isolate cancer cells, single cell suspension was sorted based on GFP expression. Cell sample was processed with the "Chromium Next GEM Single Cell 3' Reagent Kits v3.1" (10xGenomics PN-1000268). Generation of gel beads in emulsion (GEMs), barcoding, GEM-RT clean-up, cDNA amplification and library construction were all performed as recommended by the manufacturer.