title: RNA-seq analysis of Acinetobacter baumannii AB5075 and grey colony variants having a copy of ISAba13 in the K-locus
design: RNA-seq analysis of Acinetobacter baumannii AB5075 and grey colony variants having a copy of ISAba13 in the K-locus
library_construction_protocol: A. baumannii AB5075 is a clinical strain isolated from an infected tibia.Jacobs, A. C. et al. AB5075, a Highly Virulent Isolate of Acinetobacter baumannii, as a Model Strain for the Evaluation of Pathogenesis and Antimicrobial Treatments. (2014) doi:10.1128/mBio.01076-14. Cells were grown in liquid LB medium at 37 degrees with shaking. RNA was extracted using SV Total RNA Isolation System (Promega). Libraries were prepared as described by Warman et al. (2021) Nat. Microbiol.6:746-756.