title: Sv_leaf+flower_ccs
design: Full-length transcript sequencing followed the Isoseq Express Template Preparation protocol from Pacific Biosciences (Menlo Park, CA). Leaf or flower RNA samples were prepared by pooling equal amount of 4 biological replicates of both control and MeJA-treated groups. Barcoded double-stranded cDNA libraries were generated using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module, the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, MA) and the Iso-Seq Express Oligo Kit (Pacific Biosciences). SMRTbell sequencing adapters were added to the cDNAs with the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences). Library strategy is using a mixture of oligo(dT) and random primers is used to generate full-length cDNA from the total RNA.