title: GSM7984699: SK-Mel-147_ink_rep2_H3K9me3; Homo sapiens; OTHER
library_construction_protocol: After antibody binding and tagmentation, PCI was added and the solution was transferred to a phase-lock tube and centrifuged for 5 min at 16,000xg. Chloroform was added and the acqueous solution was transferred to a new tube containing ethanol. Samples were centrifuged for 15min at 16,000xg. Two additional ethanol washes were performed before drying the pellet and dissolving it in Tris-HCl pH 8 0.1mM EDTA. Libraries were prepared for sequencing using barcoded primers from Buenrostro et al. 2015. The NEBNext HiFi 2x PCR Master mix was used to perform the reaction.