title: GSM8059295: MAC2A_H3K27me3_NP11513; Homo sapiens; OTHER
library_construction_protocol: Live cells were washed and incubated with activated concanavalin A beads for 10 min at room temperature. Cells were then resuspended in antibody buffer containing 0.01% digitonin, 1 ml of each antibody was added to individual cell aliquots, and tubes were rotated at 4°C overnight. The following day, targeted chromatin digestion and release were performed with 2.5 ml of Cutana pA/G-MNase and 100 mM CaCl2. Retrieved genomic DNA was purified with a MinElute PCR purification kit and eluted in 10 ml of buffer EB. Swift 2S automated library prep (manufacturer's instructions)