title: GSM8098925: U62_siSOX10_H06; Homo sapiens; RNA-Seq
library_construction_protocol: For Novogene sequencing total RNA was extracted with NucleoSpin RNA extraction kit (MACHEREY-NAGEL) according to manufacturer's instructions, and 1500 ng/30 μL RNA was used for sequencing. For 96 well RNA sequencing libraries from each cell line were generated based on the Smart-seq2 protocol with modifications as described in the DrugMap publication. Libraries were prepared with tagmentation using the Nextera XT Library Prep kit. Libraries used custom barcode adapters and were combined for sequencing.