title: RNA-Seq of mus musculus
design: 3 L total RNA was used as a template, and NEBNext Multiplex Small RNA Library Prep Set for Illumina (Catalog NO. E7300, New England Biolabs) was used to build the library, following the manufacturers instructions with modification. The DNA library was purified by using 6 % PAGE gel, quantified by using QubitTM3 fluorometer (Invitrogen, Cat. No. Q33216) with Qubit dsDNA HS Assay Kit (Invitrogen, Cat. No. Q32854), examined by using Qsep100TM bio-fragment analyzer (Bioptic Inc., Taiwan, China), and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.