title: GSM8008938: Non Treated EC miR 409 mimic-1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using RNeasy Mini Kit (Qiagen, #74104). 500ng of total RNA was used for the construction of sequencing libraries. From 500ng starting material, the mRNA was enriched and fragmented using enzymatic fragmentation. After first and second strand synthesis, the cDNA was end-repaired and 3' adenylated. Sequencing adapters were ligated to the overhangs. Adapted molecules were enriched using by 13 cycles or PCR and purified by a bead based cleanup. The libraries size distribution was validated and quality inspected on a TapeStation (Agilent Technologies). High quality libraries are pooled based in equimolar concentrations based on the Bioanalyzer automated electrophoresis system (Agilent Technologies). The library pool(s) were quantified using qPCR and optimal concentration of the library pool used to generate the clusters on the surface of a flowcell before sequencing on a NextSeq500/550 instrument (1x75 cycles) according to the manufacturer instructions (Illumina Inc.).