title: GSM7505243: Smc6-FLAG, Input [2019_030B_3067-3_WCE]; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation was performed as detailed in Jeppsson et al., 2022. Briefly, S. cerevisiae cells were crosslinked with 1 % formaldehyde for 30 minutes at room temperature, followed by incubation at 4°C overnight. Chromatin was then sheared to a size of 300-500 bp by sonication (Bandelin Sonopuls HD 2070.2) and IP reactions, with anti-FLAG antibody (Merck, F1804) conjugated to Dynabeads Protein A (Invitrogen, 10002D), were allowed to proceed overnight at 4°C. After completing the immunoprecipitation and reversing crosslinks, the DNA was purified. For ChIP-seq, DNA from ChIP and input fractions were prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645).