title: NextSeq 500 paired end sequencing; H3K56ac profiling in Candida albicans by high throughput sequencing
design: H3K56ac profiling in Candida albicans by high throughput sequencing
library_construction_protocol: C. albicans cultures were cross-linked with 1% formaldehyde for 15 minutes at room temperature with gentle shaking. The reaction was quenched with 125 mM glycine and cells wer havested by centrifugation.  Budding yeast cell culture was diluted at a cell density of 10^5 cells/mL in YPD and incubated at 25°C for 28 h with or without 10 mM NAM. ChIP was performed as previusly described (Mawer, J., Massen, J., Reichert, C., Grabenhorst, N., Mylonas, C., and Tessarz, P. (2021). Nhp2 is areader of H2AQ105me and part of a network integrating metabolism with rRNA synthesis.EMBO reports, 22(10), e52435. doi: 10.15252/embr.202152435) except for cell lysis that was carried out by using a cryogenic freezer mill (SPEX SamplePrep 6970EFM Freezer/Mill, München, Germany). DNA from Input and ChIP samples was purified with Macherey-Nagel NucleoSpin Gel and PCR Clean-Up Kit. Libraries were generated from H3K56ac and input following a previously published protocol (Ford, E., Nikopoulou, C., Kokkalis, A., and Thanos, D. (2014). A method for generating highly multiplexed ChIP-seq libraries. BMC research notes, 7, 312. doi: 10.1186/1756-0500-7-312).