GSM7774445: Liver_ZT10_anti-HA_In-situ_ChIA-PET; Mus musculus; ChIA-PET
Isolated liver nuclei were fixed with 1% formaldehyde for 10 minutes and followed by 45 min 1.5 mM EGS. After quenching and cell lysis, nuclei were permeabilized by 0.1%SDS at 62 degree for 10min, 37 degree for 20 min, 0.55% SDS at RT for 1h. The nuclei were digested by AluI enzyme and re-ligated by T4 DNA ligase sequentially. Ligated chromatin was sheared by probe sonicator. Sheared DNA was pulldown by a specific antibody; Immunoprecipied DNA was subjected to Tn5 tagmentation, M280 Dynabeads streptavidin pull-down and PCR amplification; PCR products were subjected to size-selection and pair-end sequencing on Illumina Hiseq or Novaseq platform.