GSM7430131: YLG137, RPA ChIP, 4h, rep1; Saccharomyces cerevisiae; ChIP-Seq
Cells were mechanically lysed and chromatin was sheared by sonication to fragments of 200-500 bp. 40% of the chromatin extract was used for immunoprecipitation, the purified complexes were treated with proteinase K and crosslinks reversed. DNA was purified by phenol-chloroform extraction and ethanol precipitation. Strand-specific ChIP-seq libraries were prepared starting from 1 ng of DNA using Accel-NGS® 1S Plus Library Kit, following manufacturer instructions. 12-14 cycles were used for library amplification. Clean-up steps were performed using SPRIselect beads.