GSM8061638: Th1 EM KO D16 rep1 batch2; Homo sapiens; RNA-Seq
RNA was extracted using miRNeasy Micro Kit (Qiagen). 10ng - 1ug of total RNA was used for the construction of sequencing libraries. Poly-A-tailed messenger RNA (mRNA) was selected (NEBNext® Poly(A) mRNA Magnetic Isolation Module) from total RNA.  NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) was used to generate cDNA libraries from the isolated Th1 EM WT and KO RNA (RIN score ≥ 7). All procedures were performed according to manufacturer's protocols. NEBNext® Multiplex Oligos for Illumina (NEB) was used for barcoding individual RNA libraries, and SPRIselect (Beckman Coulter) was used for size selection and purification.