GSM7141053: plasma exosomes, HC, rep10 (miRNA); Homo sapiens; miRNA-Seq
About 5 ml of plasma was isolated from 10 ml of peripheral blood. The plasma exosome was extracted from 5 ml plasma of participants. The exosomes were isolated using the exoEasy Maxi Kit (Qiagen) following the manufacturer's protocol.Total RNA was used for removing the rRNAs with NEBNext® rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer's instructions. RNA libraries were prepared for sequencing using standard Illumina protocols