GSM8321007: muscle, PH195, week 12, 24hr; Homo sapiens; miRNA-Seq
Muscle: Total RNA isolated using mirVana Paris Kit (Fisher); Serum exosomes: Exosomes isolated using Total Exosome Isolation from Serum Kit (Life Tech) and exosomal RNA purified using the Total Exosome RNA and Protein Isolation Kit (Life Tech).  XX RNA was used for construction of sequencing libraries. cDNA was barcoded with IonExpress RNA-Seq Barcode 1-16 kit (Life Tech). 7.5picomoles each of 8-10 barcoded libraries were pooled and clonally amplified onto IonSphere Particles and enriched using the OneTouch2 (Life Tech).