GSM7142243: Tcm WT invitro stim 24h CD8 RNAseq rep1; Mus musculus; RNA-Seq
WT or CTCF-floxed Tcm cells were sort-purified from the recipient spleens, unstimulated or stimulated with GP33 peptide for 24 hrs ex vivo, and total RNA was extracted using standard Qiagen RNease columns. RNA-seq libraries were prepared for sequencing using stardard Illumina protocol. The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.