GSM7785622: BCR-ABLp210-bio repl 1; Drosophila melanogaster; RNA-Seq
For RNA-seq samples, 300 larvae were bled in 240-300 µl PBS then put in 700-760 µl of Trizol (TRI reagent, Sigma Aldrich). RNA was extracted using the TRI Reagent (Sigma-Aldrich). Three different biological replicates (N=3) with 300 third-instar larvae for each replicate were used for RNA-seq (900 larvae total) in the following conditions: W1118 (control), BCR-ABLp210 and BCR-ABLT315I. Samples were sent to Macrogen (Seoul, South Korea) for RNA-Seq analysis. Illumina TruSeq stranded total RNA with ribo-zero