GSM7729886: Old, replicate 2, scRNAseq; Homo sapiens; RNA-Seq
Peripheral blood was obtained from normoglycemic Y adults who were lean (Y; avg: 31.81 yrs; BMI 22.20 kg/m2) or normoglycemic O adults who were lean (O; avg: 68.11 yrs; BMI 23.46 kg/m2). CD4+ T cells were isolated according to our established protocol. Briefly, fifty milliliters of peripheral blood was collected into acid/citrate/dextrose containing tubes by venous puncture. PBMCs were purified by histopaque. CD4+ T cells were isolated from the PBMCs by negative selection using MACS columns (Miltenyi Biotech). Libarary was prepared according to manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).