WGS sequencing of Pseudomonas sp. strain DKN 2792
Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) following the manufacturers protocol with the following modifications: input DNA was increased 2-fold, and PCR elongation time was increased to 45s. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system (Hamilton Bonaduz AG, Switzerland). Pooled libraries were quantified using the Kapa Biosystems Library Quantification Kit for Illumina. Libraries were sequenced using an Illumina sequencer (NovaSeq 6000) using a 250bp paired end protocol. Reads were adapter trimmed using Trimmomatic 0.30 with a sliding window quality cutoff of Q15. Quality was assessed using in-house scripts combined with Samtools, BedTools and bwa-mem.