GSM8013398: GBM1, Patient1, Region1, Section4; Homo sapiens; OTHER
For ST sequencing, 16 fresh tissue samples were extracted via surgery from 3 glioblastoma patients. The GBM tumor specimens were promptly frozen in liquid nitrogen and embedded in OCT compound. The frozen tissue specimens were then cryo-sectioned at a thickness of 10 µm and were mounted on pre-treated glass slides and incubated at 37°C for 1 minute , followed by fixation in methanol for 30 min at -20°C and washing in phosphate buffered saline. The current ST protocol was applied to all sections, resulting in both conventional hematoxylin and eosin (H&E) images that were amenable to annotation by pathologists, as well as gene expression profiles for each microarray spot. Spatial Transcriptomome sequencing libraries were prepared with 10 × Genomics Visium Spatial Gene Expression Slides & Reagent Kit according to manufacturer's construction