GSM7312338: P2_B04_S112, Jurkat, Infected, plexWell; Homo sapiens; RNA-Seq
Single cell suspensions were prepared and sorted on a BD FACS Aria IIU into 96 well PCR plates that were prepared with lysis buffer according to seqWell plexWell Rapid Single Cell method (user guide v20210402) and containing ERCC spike-in mix (Invitrogen # 4456740) at a dilution of 1:1E7. Following deposition of single cells into prepared plates, they were snap-frozen and stored at -80C until further processing. Single cell cDNA and libraries were generated for each sorted sample according to seqWell plexWell Rapid Single Cell user guide. Multiplexed libraries containing indexed single cell RNA-Seq libraries were sequenced on the either the NextSeq 2000 or NovaSeq 6000 with a target read depth of 2 million reads per cell using the following read parameters: Read 1 61bp, Index 1 8bp, Index 2 8bp, Read 2 61bp.