Non tumorigenic keratinocytes transduced with E6/E7 from HPV18
Total RNA extraction was achieved on transduced keratinocytes infected with lentiviral particles that bear the E6/E7 genes of HPV16 or 18; this process was addressed in duplicate. The keratinocytes transduced with the empty pLVX vector were used as calibrator in the comparison of each of the cell models. After that, the two replicates were compared as a single end of each model vs. the control for the development of the differential expression analysis.