GSM7676130: SCS macrophages, mRNA vaccine, 24h; Mus musculus; RNA-Seq
Total RNA was extracted and purified from the sorted cells (>1000 cells) using an RNeasy micro kit (Qiagen) following the manufacturer's instructions and converted into cDNA using a SMART-Seq HT kit (Takara). Sequencing libraries were generated using Nextera XT DNA Library Preparation Kit (Illumina).