GSM8061230: M45_ZT18_Nac_S21; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Plus Micro Kit (Qiagen) RNA quantity and quality were assessed using fluorometry (Qubit RNA Broad Range Assay Kit and Fluorometer; Invitrogen) and chromatography (Bioanalyzer and RNA 6000 Nano Kit; Agilent), respectively. Libraries were prepared using the Smartseq stranded total RNA ultra-low input sample preparation kits (Illumina).