GSM7310880: blank_1; blank sample; OTHER
hyb protocol: Slides were incubated with a cocktail of barcoded ISH probes overnight at 37C in a hybridization oven, and then at RT blocked for 30 minutes then incubated for 1 hour (also RT) with a cocktail of DNA stain (SYTO13) with fluorescent antibodies. scan protocol: Slides were scanned at low resolution on the GeoMx to identify tissue regions, and then High-Resolution scans using the 20X objective were performed using the pre-built scanning conditions for the Fluorescent antibody kit from Nanostring. Samples were pooled during library preparation. Individual FASTQ files were generated for each ROI, forward & reverse primers.