GSM6742505: NSPC, 3xTg, rep1; Mus musculus; RNA-Seq
A tamoxifen (TAM)-inducible Nestin-CreERT2;ROSA26-EYFP reporter mouse line  was cross-bred with NTG or 3xTG-AD mice to label NSPC upon TAM administration. YFP-positive NSPCs were sorted through FACS (Fluorescence-Activated Cell Sorting) 10 days after TAM from NTG; Nestin-CreERT2;ROSA26-EYFP and 3xTg-AD;Nestin-CreERT2;ROSA26-EYFP mice. Subgranular zone (SGZ) was removed and digested. YFP-positive cells were sorted through FACS. RNA was extracted with RNeasy Micro Kit. cDNA library was estalished using Takara  SMART-Seq V4 cDNA synthesis / Nextera XT library prep.