GSM7317635: ΔUBR4, sgTIMM8A, biol rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using the NucleoSpin RNA kit (Macherey-Nagel) mRNA was purified from total RNA using polyT oliog attached magnetic beads. mRNA was fragmented and first strand synthesis was performed with random hexamers followed by second strand cDNA synthesis. This was followed by end repair, A-tailling, adapter ligation, size selection, amplification and purification.