GSM7139782: NCIH460-ATACseq-biol_rep_2; Homo sapiens; ATAC-seq
Samples were purified using the Qiagen Mini elute PCR Purification kit Libraries were PCR-amplified using the NEBNext High-Fidelity PCR Master Mix and amplified for 5 cycles using NEBNext 2x MasterMix and custom primers.Libraries were sufficiently amplified individually in addition to 5 cycles of PCR as computed from qPCR fluorescence curves. Libraries were purified using Zymo DNA Clean and Concentrator. The libraries were then size selected withwith a 1.5× AMPure XP Bead: DNA ratio. Final libraries were eluted and sequenced using Illumina Nextseq 550 System with NextSeq 500/550 mid Output Kit v2.5.