RNA-Seq of liver:cow
On day 21, three dairy cows per group were selected randomly. The liver samples were obtained as described in a previous study [27]. Then, total RNA from liver tissue was prepared using TRIzol (Life Technologies, CN, China) and examined the quality and integrity as previous description [27]. cDNA library preparation and sequencing were conducted according to the description from Ren et al., [28]. Then, the PCR products were purified and library quality was assessed on the Agilent Bioanalyzer 2100 system and qualified by the ABI StepOnePlus Real-time PCR system. Finally, the amplified library was sequenced on an Illumina HiSeqTM 2004 sequencing machine.