RNA-seq of Tagetes erecta:flower, biological replicate D1
High-quality total RNA was extracted from fresh flower, quality checked by agarose gel electrophoresis and Nanodrop 2000 spectrophotometer, quantified by Qubit 2.0 and Agilent 2100 bioanalyzer. The mRNA was enriched by Oligo-dT beads, fragmented and reverse transcripted into cDNA and purified by AMPure XP beads, end-repaired, A-tailed, and adapter-ligated, purified and amplified by Illumina TruSeq RNA Library Prep Kit. The prepared library was quantified by Qubit 2.0 and Q-PCR and quality checked by Agilent 2100 before loading onto flowcell