GSM7426676: Wing disc, HP1a-chromo, HiC, rep1; Drosophila melanogaster; Hi-C
Each in situ Hi-C replicate was performed using 60 wing imaginal discs dissected from female H3.2HWT, H3.2K9R, HP1-chromo, and HP1-control wandering L3 larvae. Discs were fixed in 1xPBS with 4% paraformaldehyde for 20min, transferred to tubes in a minimal volume of 1xPBS plus 0.15% Triton X-100, and used as input for the Arima Hi-C kit v00 protocol (A410030), following manufacturer's instructions that were last revised March 2018. Hi-C libraries were amplified using the KAPA Hyper Prep kit (KK8502).