GSM7946872: C. elegans, mbl-1c(syb5299) mutant, L4, IP sample, rep 1; Caenorhabditis elegans; RIP-Seq
TRIzol RNA purification Unenriched RNA samples were fragmented to 250 bp and reverse transcribed to cDNA with random primers. cDNAs were dA-tailed and DNA fragments with A tail were ligated to sequencing adaptors. Final DNA library was obtained by PCR amplification. After the construction of the library, the initial quantification was done with Qubit 2.0, and the library was diluted to 1ng/l. Then the insertion size of the library was detected with NGS3K. If meeting the expectation, the accurate concentration of the library was quantified by Q-PCR (library effective concentration > 2nM) to ensure the quality of the library.