GSM8105685: D4_endocx; Macaca mulatta; RNA-Seq
For bulk RNA sequencing experiments, reproductive tract tissue was isolated directly into RNAlater (Qiagen). Tissue was homogenized in Trizol (Sigma-Aldrich) using a Tissuemiser homogenizer (Fisher Scientific), and RNA was extracted using a RNeasy mini kit as per the manufacturer's instructions (QIAGEN). RNA integrity was assessed using capillary electrophoresis with the Agilent 2100 BioAnalyzer system (Agilent Technologies). Sequencing libraries for CM9-specific CD8 T cells were prepared using the Clontech SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian kit. Libraries for whole tissue cell extracts were prepared using the TruSeq stranded mRNA library prep kit (Illumina).