GSM7849335: HMGA1_H1299_DoxshHMGA1_3; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde at room temperature for 10 min (RT) or at 4°C for 1 hour (4C). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7645L) according to the manufacturer's instructions except that size selection was performed after PCR amplification using AMPure XP beads (Beckman Coulter, A63881). Samples were sequenced paired-end using 50 bp reads on the Illumina platforms.