GSM7094527: HA+L3  rep3; Homo sapiens; RNA-Seq
Total RNA was extracted using TransZol Up following the manual (TransGen, Beijing, China). RNA-seq libraries were prepared using MGI's DNBSEQStr and-Specific Transcriptom Kit following the manufacturer's instructions. Briefly, poly(A)-tailed mRNA was purified and fragmented, followed by cDNA synthesis, end repair, adapter ligation, and PCR amplification. The quality and quantity of the libraries were assessed using a Qubit fluorometer and an Agilent Bioanalyzer.