POLDIP2 is required for mtDNA elimination during spermatogenesis to ensure maternal inheritance in Drosophila melanogaster
POLDIP2 is required for mtDNA elimination during spermatogenesis to ensure maternal inheritance in Drosophila melanogaster
To extract genomic DNA for sequencing, 10 adult males were homogenised in 500 μl of solution A buffer (0.1 M Tris-Hcl pH 7.5, 0.01 M EDTA pH 8.0, 1% SDS) and incubated for 20 min at 70°C. 70μl of 8M potassium acetate was then added to the samples and incubated for 20 min on ice. After the incubation, the samples were centrifuged for 15 min at maximum speed.  The supernatant was mixed with 250 μl of isopropanol and centrifuged for 4 min at 13,000 g. The pellet was washed with 70% ethanol and diluted in 100 μl ddH2O.  The DNA library was prepared using the Illumina DNA Prep kit (Illumina, 20018705) and index kit (Illumina, 20027213) following the manufacturer's instructions.