GSM7848616: KRV36, light-adapted cells, 4 hours after the shift to dark, rep1; Sediminicoccus rosea; RNA-Seq
Cells from triplicate cultures were harvested by centrifugation, resuspended in 1 mL PGTX extraction solution (Pinto et al., 2009) and immediately frozen in liquid nitrogen. RNA was extracted as described earlier (Kopejtka et al., 2020). Libraries were generated according to the protocol of Shishkin et al. (2015) including rRNA removal with the RiboZero Kit (Illumina, USA) and sequenced on a NovaSeq 6000 (Illumina, USA) in paired-end mode with 100 cycles in total.