GSM7316349: ATAC-seq_WT_rep2; Mus musculus; ATAC-seq
5×104 viable cells were re-suspended in 50 μl cold ATAC-Resuspension Buffer (10 mM Tris-HCl PH 7.4, 10mM NaCl, 3mM MgCl and 0.1% NP40) and incubated on ice for 3 minutes. The nuclei were pelleted at 500 RCF for 10 min at 4°C, then re-suspended cell pellet in 50 μl of transposition mixture and incubated reaction at 37°C for 30 min in a thermomixer with 1000 rpm mixing. The ATAC-seq was performed using TruePrep DNA Library Prep Kit V2 for Illumina (TD501, Vazyme, China). 200~1000bp fragmented DNA was selected and enriched using AMpure XP (Beckman Coulter, Inc. American).