Illumina HiSeq 2000 sequencing; RNA sequencing of beech roots and associated fungi
RNA sequencing of beech roots and associated fungi
Per plant: harvest of fine roots (< 2mm diameter) in the field, froozen in liquid nitrogen and transport to the lab on dry ice. Storage at -80°C until RNA extraction environmental samples Total RNA was extracted using the protocol of Chang S, Puryear J, Cairney J (1993, A simple and efficient method for  isolating RNA from pine trees. Plant Mol Biol Rep 11:113–116). The RNA of four samples was pooled. The cDNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt am Main, Germany) according to the manufactorer´s protocol