GSM7774641: Kidney transplant nephrectomy, G10, scTCR; Homo sapiens; OTHER
Isolation of CD4 TRM and CD8 TRM cells was performed using a FACS Aria II cell sorter, using the following fluorescent antibodies (CD45, CD3, CD4, CD19, CD69, CD103, CD49a, CD11b, CD16 and CD127). Libraries were prepared on a Chromium Controller using the Chromium Next GEM Single Cell 5' Reagent Kit V2 in combination with the Chromium Single Cell Human TCR Amplification Kit (10x Genomics, Pleasanton, CA, USA). In this way, single-cell transcriptomes and immune profiles were generated from the same sample. Next-generation sequencing (26-10-10-90 cycles) of both libraries (transcriptome and TCR-enriched) was performed on an Illumina NovaSeq 6000 platform.