GSM7311579: PCS-201-021 P4 DMSO 2; Homo sapiens; RNA-Seq
RNeasy® Plus Mini Kit (Qiagen, Germany) was used to isolate RNA following the manufacturer's protocol. Once RNA isolation was complete, the RNA concentration was calculated using the NanoDrop™ (NanoDrop 2000 Spectrophotometer, ThermoScientific). 100 nanograms of total RNA from each sample was enriched for polyadenylated RNA and prepared for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) according to the manufacturer's instructions.