GSM7428172: Esophagus, KO7; Mus musculus; RNA-Seq
Extraction of RNA from whole tissue was performed on tissue stored in RNA-later (Sigma-Aldrich)until homogenization using a Tissue-Tearor (Dremel, Racine, WI), followed by phenol/chloroform extraction as described (28085883) and clean-up with the Rneasy Mini Kit with on-column DNAse digestion according to manufacturers' instruction. RNA QC (average RIN 8.8). mRNA enrichment and cDNA library prep utilizing the stranded mRNA (poly-A selected) library preparation kit.