GSM8146615: B16F10 tumor, rev day 5, replicate 2, bulkRNAseq; Mus musculus; RNA-Seq
Prior to tumor inoculation, B16F10 CD47 KO cells were treated with 2.5 μM MPS1i (reversine) or the equivalent volume of DMSO vehicle control. Cells were treated for 24 h, after which they were washed twice with PBS and allowed to recover for an additional 48 h. After the recovery period elapsed, all mice were subcutaneously injected with 5x105 B16F10 cells. Five or ten days after tumor inoculation, mice were euthanized, and tumors were excised and disaggregated for bulk RNA-sequencing. RNA library was constructed using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina (E7770S, E7335) per the manufacturer's instructions.