GSM7140720: Negative_1; Toxoplasma gondii; OTHER
Ten million (1x10^7) parasites were centrifuged 2000xg for 10min and the parasite pellet used directly with the CUT&Tag-IT Assay Kit (Active Motif 53160). Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line) with the same primary antibody conditions. Samples were incubated with primary antibody overnight at 4°C. After tagmentation, DNA was extracted, purified on a column and libraries were amplified by PCR usinf i5&i& indexed primers