RNA and DNA Metavirome data of Leech Hirudinaria manillensis
Add modified DNA polymerase and dNTP with 4 fluorescent markers to synthesize one base per cycle. Scan the surface of the reaction plate with a laser and read the type of nucleotide that each template sequence polymerizes in the first reaction. Scan the surface of the reaction plate with a laser and read the type of nucleotide that each template sequence polymerized in the first round of reactions. Determine the sequence of the template DNA fragments by calculating the fluorescent signal collected in each round of reactions.