GSM7749627: Kidney Medulla-Hi-C-Donor5; Homo sapiens; Hi-C
Approximately 0.5 cm3 of tissue was minced with a razor blade and fixed and nutated for 20 minutes in 10 mls of a 1:10 dilution of 10% neutral buffered formalin (Fisher). Following this, the formalin was quenched by direct addition of 0.1 g of glycine (Sigma) and nutation for another 15 minutes. The tissue fragments were submitted to Phase Genomics for Hi-C library preparation using their Human Hi-C Kit and sequencing on an Illumina HiSeq 4000.