GSM8242235: HS-SY-II, H3K27ac, ChIP, input; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 min. After quenching and cell lysis, chromatin was sonicated using the Bioruptor sonicator from Diagenode. Approximately 0.1ng to 20ng ChIP DNA was fragmented to size range around 200bps using Covaris (shearing parameter 2 minutes in LV tubes using 15ul microTUBE-15 AFA Beads Screw-cap, Cat: #520145), and used for ChIP-seq library preparation by following the NEBNext Ultra II DNA library preparation Kit for Illumina (Cat. No: E7645L, New England Biolabs Inc, Ipswich, MA, USA). This kit utilizes a proprietary adapter attachment chemistry which minimizes bias and supports very low amount of input sample.