NextSeq 550 paired end sequencing; Extracellular Vesicles in acute coronary syndrome: carrier of epigenetics marks
Extracellular Vesicles in acute coronary syndrome: carrier of epigenetics marks
Circulating Extracellular vesicles (Evs) were recovered from plasma samples. Circulating Extracellular vesicles (Evs) were recovered, by ultracentrifugation, from plasma samples of 19 Acute coronary syndromes  patients and 50 healthy subjects (HS). Nanoparticle tracking analysis (NTA) and western blot (WB) were used to confirm the EVs integrity and purity. Peripheral blood mononuclear cells (PBMCs) of volunteer donors were treated with both ACS and HS derived EVs and genomic DNA was extracted to perform epigenome wide analysis through Reduced Representation Bisulfite Sequencing. The Ovation RRBS Methyl-Seq System features a streamlined workflow consisting of five main steps: MspI digestion, adaptor ligation, final repair, bisulfite conversion, and PCR amplification to produce the final library.