Amplicon-based panel of Homo sapiens: FFPE sample
Lymph node gDNA and plasma cfDNA baseline samples were screened for mutations with a short-length Ampliseq Custom Panel (Thermo Fisher Scientific). The panel, established as a routinary diagnosis tool at the H12O, was designed to cover all coding regions of 56 lymphoma-specific genes in FFPE samples. Samples were sequenced with an average coverage of 2,150x on an Ion S5 System platform (Life Technologies, ThermoFisher Scientific).