GSM7426216: Neutrophil, bystander, exp 2; Mus musculus; RNA-Seq
Cells were lysed in TRIzol Reagent and phase separation was induced with chloroform. RNA was precipitated with isopropanol and linear acrylamide, washed with 75% ethanol and resuspended in nuclease-free water. 10 ng total RNA with RNA integrity numbers ranging from 8.8 to 9.5 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit from Clonetech following the manufacture's protocol. Subsequently, 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems).