Deep sequencing of BlaC
Samples for Illumina sequencing were prepared by PCR using plasmid libraries as templates. Amplicons were barcoded using universal tail sequences and sequenced by a commercial partner on either an Illumina MiSeq sequencer. Samples were sequenced paired-end 300-bp using 600 cycle v3 sequencing reagents. FASTQ files were generated using bcl2fastq v2.20. Merging is required before considering target region offset (read length after merging should be 465 bp).