GSM7677917: Gli1- MuSCs-3; Mus musculus; RNA-Seq
Total RNA was extracted from Gli1+ or Gli1- MuSCs using Trizol reagent (Invitrogen)  according to the manufacturer's instructions. The integrity of the isolated RNA was verified using a Bioanalyzer (Agilent 2100). Library construction was performed with 1 ng of input total RNA using the VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme) according to the manufacturer's instructions and sequenced on an Illumina NextSeq sequencer.