16S rRNA amplicon seq of bacteria: endospheric layer
Total metagenomic DNA was extracted from rhizospheric soil of every biological replicate by using the PowerSoil DNA Isolation Kit, and examined by using 1% agarose gel electrophoresis before the DNA samples were stored at 20C in a freezer.Then an improved dual-index high-throughput sequencing approach with paired-end 250 nt was used to create the sequencing library of 16S rRNA gene V3-V4 region.