GSM8322425: Zbtb20-cKO cells, IL-1β treatment_2; Mus musculus; RNA-Seq
The chondrocytes treated by 1ng/mL IL-1β or not were harvested and lysed in TRIzol reagent (Invitrogen). The purified mRNA was quantified and qualified using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). cDNA library was prepared using NEBNext® Ultra™ RNA Library Prep Kit for Illumina®