GSM8101640: Vehicle control; Homo sapiens; RNA-Seq
Cell concentration and viability of mouse samples were measured using a hemocytomter and ranged from 910,000 - 1,180,000 cells/mL with ~90% viability. Cell samples were input into 10x Genomics Single Cell 3′ v3.1D workflow and processed according to the manufacturer's instructions for a targeted recovery of 6000 cells (10X Genomics, USA). The resulting gene expression libraries were sequenced on an Illumina NextSeq 2000 instrument with 850pM input and 2% PhiX control library spike-in (Illumina, USA). scRNA-seq libraries were generated in BU DOM Single Cell sequencing core facility and sequenced by BU Microarray and Sequencing Resource Core Facility according to 10x Genomics protocol