RNA-Seq of Ba/F3-CLIP1-LTK cell line
Total RNA was used as input material for the RNA sample preparations. Briefly, mRNA waspurified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation wascarried out using divalent cations under elevated temperature in First Strand SynthesisReaction Buffer(5X). First strand cDNA was synthesized using random hexamer primer andM-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequentlyperformed using DNA Polymerase I and RNase H. Remaining overhangs were converted intoblunt ends via exonuclease/polymerase activities. After adenylation of 3 ends of DNAfragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. Inorder to select cDNA fragments of preferentially 370~420 bp in length, the library fragmentswere purified with AMPure XP system (Beckman Coulter, Beverly, USA). Then PCR wasperformed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index(X) Primer. At last, PCR products were purified (AMPure XP system) and library quality wasassessed on the Agilent Bioanalyzer 2100 system.