GSM7774099: 220718L32_exp220601_MS80_SCGE_gDNA-seq-rep2; Saccharomyces cerevisiae; Nakaseomyces glabratus; OTHER
S. cerevisiae and C. glabrata cells were separately pelleted and snap-frozen in liquid nitrogen. Pellets were thawed in ice-cold PBS and mixed. 20 µl of cells in PBS were added to 110 µl YD Digestion Buffer (YeaStar Genomic DNA Kit; ZymoResarch) and gDNA was extracted according to Protocol 1 for the YeaStar Genomic DNA Kit (Zymo Research). 4ng gDNA was used for prepared for sequencing by tagmentation with custom-made Tn5 (30 minutes at 37 °C) and then cleaned up (MinElute PCR Purification Kit, Quiagen). Tagmented DNA was amplified (7 cycles) with NEBNext High-Fidelity 2X PCR Master Mix (M0541). Libraries were then cleaned up using AMpure XP beads (Beckman Coulter), pooled, and sequenced by paired-end (2x150 bp) Illumina sequencing. Tn5 gDNA-seq