WGBS of tomato: WT, rep 1
Genomic DNA was isolated with an extraction buffer containing 2% sarcosyl, 10 mM EDTA, 100 mM NaCl, 2% SDS, 5M urea, 0.1% sodium-metabisulfite at room temperature, purified with phenol:chloroform:isoamyl alcohol (25:24:1, pH 8.0), precipitated in ethanol and resuspended in TE buffer. The samples were RNase treated, then phenol/chloroform extracted, precipitated again with ethanol, and resuspended in nuclease-free water. The sequencing libraries were prepared by Novogene.