RNA seq of HCT116 p53 null p73 knockdown
The  library was prepared using the Illumina TruSeq stranded mRNA library preparation kit.  Briefly,  mRNA was enriched from total RNA, followed by fragmentation. The fragmented mRNA was converted into first-strand cDNA, followed by second-strand generation, A-tailing, adapter ligation, and finally a limited number of PCR amplifications of the adaptor-ligated libraries.