RNA nifH from the Baltic Sea MP6.Deep size fraction 0.2-3um
Size-fractionated RNA samples were obtained from Baltic Sea water and extracted using RNeasy Mini kit (Qiagen) with modifications. Reversed transcribed with nifH3 primer and SuperScriptTM IV. Nested PCR approach: first reaction nifH3 and nifH4 primers: Second reaction nifH1 and nifH2 primer.