GSM6742530: human, 5-ALA+, sample 0826; Homo sapiens; RNA-Seq
Collected tissue samples were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) on gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer's instructions. mRNA molecues were captured using bead-bound oligo dT(30). Captured mRNA molecues were reverse transcriped to obtain cDNA which is then amplified by PCR reactions. The cDNA PCR reaction product was then purified with AMPure XP beads. The purified cDNA PCR product was used as the input for Nextera XT reactions to generate sequencing library. scRNA-seq libraries were prepared using Nextera XT DNALibrary Prep Kit following manufacturer's protocols.