GSM7774512: Liver_ZT22_3HAKI_anti-HA_ChIP-seq-rep4; Mus musculus; ChIP-Seq
Isolated Liver nuclei were fixed with 1% formaldehyde for 10 minutes and followed by 45 min 1.5 mM EGS. After quenching and cell lysis, chromatin was sheared by probe sonicator. ChIP-seq libraries were prepared by Tn5-mediated tagmentation and adapter ligation. Adapted DNA was amplified by PCR and size selected for final sequencing.