GSM7678113: 7363; Mus musculus; RNA-Seq
Islets were isolated using the standard collagenase/protease digestion method. The pancreatic duct was cannulated and distended with 4°C collagenase/protease solution. Islets were separated from the exocrine tissue using Histopaque-1077. RNA from sorted beta cells was extracted. RNA libraries were prepared for sequencing using standard Illumina protocols