Illumina NovaSeq 6000 paired end sequencing of SAMD00590218
"ChIP assays were conducted with the use of SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads, Cell Signaling Technology) according to the manufacturer protocol. Cross-linked chromatin prepared from 4 x 10e6 cells was immunoprecipitated overnight with antibodies against EZH2 (Cell Signaling Technology, #5246S), H3K27me3 (Cell Signaling Technology, #9733S) or TAp73 (Abcam, #ab14430). The immunocomplexes were then conjugated with ChIP-Grade Protein G Magnetic Beads (Cell Signaling Technology) for 2 hours at 4C. After 4 washes, the bead-bound DNA was eluted by gentle vortexing (1,200 rpm) for 30 minutes at 65C. Purified DNAs and the input were subjected to ChIP-seq. The TruSeq ChIP Library Preparation Kit (Illumina) was used for library preparation. Libraries were sequenced on the Illumina NovaSeq6000 platform with a standard 150-bp paired-end read protocol."