GSM7677993: Tgd.TNFa.Sp_3; Mus musculus; RNA-Seq
At 2 hours post-injection, 1,000 cells of the 14 immune cell-types from spleen and peritoneal cavity were double-sorted in 5uL of TCL Buffer (QIAGEN) containing 1% 2-mercaptoethanol (Sigma) by BD FACS Aria Library construction and sequencing and data processing were performed according to the standard Immunological Genome Project protocols (https://www.immgen.org/img/Protocols/ImmGenULI_RNAseq_methods.pdf)