GSM7506180: Bhlhe40 KO Ly49Hpos NK cells, d2 MCMV, biol rep 2; Mus musculus; RNA-Seq
Total RNA was extracted from sorted NK cells using PicoPure RNA Isolation Kit (Thermo Fisher Scientific). ). After RiboGreen quantification and quality control by Agilent BioAnalyzer, 2 ng total RNA with RNA integrity numbers ranging from 8.4 to 10 underwent amplification using the SMART-Seq v4 Ultra Low Input RNA Kit (Clonetech catalog # 63488), with 12 cycles of amplification. Subsequently, 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 in a PE50 run, using the HiSeq 3000/4000 SBS Kit (Illumina).  An average of 36 million paired reads were generated per sample and the percent of mRNA bases per sample ranged from 60% to 71%.