Stress 72h-First paired-end file R3
We isolated the Total RNA from Sample of interest (Tissue). Then eliminated DNA contamination using DNase. After that choose an appropriate kit for library prep process depending on the types of RNA. For mRNA with poly-A tail, used mRNA purification kit; for non-coding RNAs, such as lincRNA, used ribo-zero RNA removal Kit to purify RNA of interest. Then randomly fragment purified RNA for short read sequencing were selected. Reverse transcribed fragmented RNA into cDNA. Then ligated adapters onto both ends of the cDNA fragments. After amplifying fragments using PCR, selected fragments with insert sizes between 200-400 bp. For paired-end sequencing, both ends of the cDNA were sequenced by the read length.