GSM8060338: morc1_csr1RNAi_F4_input_1; Caenorhabditis elegans; ChIP-Seq
Purified germline nuclei ChIP: the 56 hour time point, the worms were cleansed via sucrose floatation. They were then live-crosslinked in 2.6% formaldehyde for 30 min at room temperature with nutation. The cross-linker was quenched with a 5 min nutation in glycine at a final concentration of 125 mM. Worms were washed in water and flash frozen in liquid nitrogen as ~1 mL pellets. Germline nuclei were purified and ChIP was performed according to Han, et al., 2019 with some modifications (see methods). For whole worm ChIP: chromatin sonication, immunoprecipitation, decrosslinking and DNA extraction were performed as in Weiser et al. 2017. For each immunoprecipitated sample, an input library was generated from 10% of the chromatin pre-IP and was de-crosslinked and extracted in parallel to the immunoprecipitated samples. Libraries were prepared using the Ovation Ultra Low System V2 kit (NuGEN) according to manufacturer's instructions and then sequenced on the NovaSeq6000 Sequencer (Illumina).