GSM7729806: DMSO; Mus musculus; RNA-Seq
Activated CD8+ T cells were harvested after 48 hours following in vitro T cell activation and treatment. T cells were lysed with RLT containing 2-mercaptoethanol (Sigma #M7154-24ML) and passed through a QIAshredder (Qiagen #79656) column before extraction using a RNeasy mini kit (Qiagen #74106) following the manufacturer's instructions with an on-column DNase digestion step (Qiagen #79254) Chromium next GEM single-cell 3' reagent kit v3.1 (10x Genomics #1000269) following the manufacturer's instructions