GSM7316124: PBMCs_LPS_AS1802; Homo sapiens; RNA-Seq
1x106 PBMC were cultured in 200 μl medium in non-tissue culture treated 96-well round bottom plates (Corning) and left unstimulated or treated with 200 ng/ml LPS (Enzo Bioscience) or anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) for 16 h before being prepared for sequencing. After cell culture, cells were harvested and washed in PBS with 0.1% BSA. 20,000 cells from each condition were put into a channel of Chromium Next GEM Chip K of 10x Genomics platform. Single cell RNA-sequencing libraries were generated using Chromium Next GEM Single Cell 5' Kit v2 kit according to the manufacturer's instruction.