GSM8323162: Keratinocytes, bio rep 2_day0_shSFRP1; Homo sapiens; RNA-Seq
RNA was harvest using Rneasy mini plus kit (Qiagen). 2 µg of total RNA was used for the construction of sequencing libraries. RNA library prepartion was conducted at Azenta Life Sciences with PolyA selection, using the NEBNext Ultra II RNA Library Prep Kit for Illumina, with manufacturer's instruction (NEB, Ipswich, MA, USA).