GSM7099064: MuSC-Young3; Mus musculus; OTHER
Cells were permeabilised with 0.1% digitonin. MuSCs were freshly isolated from young (4mos) and aged (24mos) mouse skeletal muscles. For H4K20me1 CUT&Tag, we used three young male mice and three aged male mice as individual replicate; in addition, one young and one age mouse were used as “No Antibody” control. CUT&Tag was performed following Henikoff's group original protocol (Kaya-Okur et al., 2019). Precipitated DNA was used for a short PCR pulse (5 cycles) using one barcode pair for all samples. For quality assessment prior to library construction, PCR product was used for qPCR; libraries were balanced based on qPCR amplification. To generate library, we performed a 13 cycles PCR with individual barcode pairs for each sample, followed by AMpure XP beads (Beckman Coulter, Inc.) cleanup. Libraries were sent to GeneWiz for 150bp pair-end sequencing.