PML::RARA_WT_ATAC-seq_replicate_3_female
Bone marrow cells from C57Bl/6 mice were lineage-depleted and retrovirally transduced with murine stem cell virus (MSCV) vectors containing an internal ribosome entry site (IRES) followed by a GFP cDNA to allow for the purification of transduced cells. These vectors either contained no insert ("empty vector"), WT PML::RARA cDNA (PR or PML::RARA_WT), or a C88A mutant in the RARA domain of PML::RARA (C88A or PML::RARA_C88A-V5). Seven days following the transduction, GFP+ cells were flow-purified, and ATAC-seq was performed.