GSM7729727: Cardiac leukocytes, UTY, scATAC; Mus musculus; ATAC-seq
Nuclei were isolated from single cell suspensions by washing once in Cell Buffer (PBS with 0.4% BSA), pelleting and resuspending in Lysis Buffer (10 mM Tris-HCl pH 7.4, Sigma Cat. #T2194l; 10 mM NaCl, Sigma Cat. #59222C; MgCl2, Sigma Cat #M1028; 0.1% Tween-20; 0.1% Nonidet P40 Substitute, Sigma Cat. #74385; 0.01% Digitonin, Thermo Fisher Cat. #BN2006; 1% BSA, MACS Cat. #130-091-376; in nuclease-free water) for 3 min on ice, washed once in Wash Buffer (same as Lysis Buffer without Nonident P40 Substitute or Digitonin), and resuspended in Nuclei Buffer (10x Genomics, Cat. #PN-2000153). Nuclei samples were processed for single-cell multiomics library preparation using the Chromium Next GEM Single Cell Multiome ATAC+ Gene Expression Kit from 10X Genomics