Whole genome sequencing of HAC Cells: Clone 2
Membrane-permeabilized cells were subjected to a 10-40% sucrose gradient to pellet nuclei. A top fraction was moved to a 22-30% sucrose gradient for further enrichment. Material from the interface was phenol chloroform extracted and then ethanol precipitated before prepping with Illumina on-bead tagmentation. Indexed with IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples). 12 PCR cycles were used for amplification. Each enriched library was pooled with the unenriched libraries at a ratio of 1 each enriched : 24 each unenriched. This pooled library was loaded on an S2 flow cell.