HI_KO3_mosaic_clawless
RNA of three wildtype toes and three knockout toes was subjected to RNA-sequencing. Sequencing libraries were be prepared using the NEB Next Poly(A) mRNA Magnetic Isolation Module and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries were  sequenced on a NextSeq500 instrument (Illumina) in 1x75bp single-end sequencing mode. 33-37 million reads per sample were generated.