GSM8013027: Ubc9-RR, Smt3, ChIP, Replicate 1; Saccharomyces cerevisiae; ChIP-Seq
Formaldehyde was added to mid-log phase cells at a final concentration of 1% for 20 min. After quenching and cell lysis, chromatin was sonicated using a VCX130 Ultrasonic processor (Sonics and Materials) ChIP-seq libraries were prepared using the TruSeq DNA Sample prep kit according to manufacturer's guidelines