GSM7312200: D28_NK_EHMT1_gRNA01_rep03 [bulk ATAC-seq]; Homo sapiens; ATAC-seq
DAPI-CD56+CD3- NK cells were isolated by FACS on Day 28 of in vitro differentiation and at least 50,000 cells/sample were used for ATAC-Seq. Nuclei isolation, tagmentation and library construction was performed as described in the Omni-ATAC-Seq protocol (Corces et al. 2017). The concentration of the final ATAC-Seq libraries was quantified using a High Sensitivity DNA Bioanalyzer Assay (Agilent) in the size range of 100-1000 bp. The libraries were then equimolar pooled and shallow sequenced on a MiniSeq to determine the quality of the libraries. The ATAC-Seq libraries were then deep sequenced on a NextSeq 550 with a 150-cycle High Output Kit v2 with the following read configuration: 76-8-8-75.