RNA-Seq of G. huxleyi UGA13 (E. huxleyi) from the outer shelf of the South Atlantic Bight: iron limited
Cultures were filtered onto a 3 m polycarbonate filter, and a Qiagen RNeasy Plant Mini kit was used to extract RNA following the manufacturer's protocol, modified to include glass bead disruption.  Sequencing was performed at the Georgia Genomics and Bioinformatics Core (GGBC) at the University of Georgia. A KAPA stranded mRNA-Seq kit was used to prepare libraries with poly-A selected reads. Tthe Illumina NextSeq 2000 platform was performed with 300 cycles and 150 paired end reads.