GSM7728300: Heat_29_1; Drosophila melanogaster; OTHER
Total RNA from S2R+ cells was isolated with Trizol (Invitrogen). 150 ng of total RNA were subjected to alkaline hydrolysis for 16 min at 96°C followed by ethanol precipitation. The extremities of RNA fragments were end-repaired and converted to libraries using NEBNext Small RNA library preparation kit according to the manufacturer's recommendations. Libraries were quantified and multiplexed and subjected for high-throughput sequencing on a NextSeq2000 instrument with a 50 bp single read mode.