GSM7312450: R01N - RNA-Seq human colorectal healthy mucosa; Homo sapiens; RNA-Seq
RNA was isolated by Rneasy mini kit (Qiagen) with RNase-Free DNase Set (Qiagen). 1.0 ug of total RNA was used for the construction of sequencing libraries. Library was prepared by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA) following manufacturer's protocols. Ribosomal RNAs (rRNAs) were removed using NEBNext rRNA Depletion Kit (Human/Mouse/Rat, New England Biolabs, USA).