GSM8322752: K562, replicate 2 HTO; Homo sapiens; RNA-Seq
We performed scRNA-seq (10x Genomics Chromium Single Cell 3' Gene Expression v3 with Feature Barcoding technology for CRISPR screening) 6-7 days post-transduction. Before the run, cell viability was determined (>95%). We leveraged Cell Hashing in order to super-load (~40,000 cells/lane) the Chromium instrument Gene expression and sgRNA feature libraries were constructed following the manufacturer's protocol. Cell Hashing libraries (Hashtag-derived oligos, HTOs) libraries were prepared following the recommended protocol on cite-seq.com