GSM7594774: NPHS2Cre:Epac1fl/fl_NTS_Day4_1; Mus musculus; RNA-Seq
After euthanasia, kidneys from (day4) or (day11) were collected and embedded in matrix (O.C.T.) and snap-frozen in liquid nitrogen. Glomeruli were isolated from 10 µM cryosections by laser microdissection (Arcturus XT, Exilon), collected on Cupules CapSure® Macro LCM (Excilone, LCM 0211) and RNA was isolated with PicoPure® RNA isolation kit (Excilone). RNA library preparation were realized following manufacturer's recommendations (QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina from LEXOGEN).