GSM8012952: CD4 T-cells, siMCU, biol rep 2; Homo sapiens; RNA-Seq
Total RNA from human CD4+ T-cells was isolated using the NucleoSpinTM RNA Plus kit (Macherey-Nagel, Cat# 740984.50), according to manufacturer's instructions. RNA isolation from rat CD4+ T-cells was performed using the RNAEasy isolation kit (Qiagen, Cat# 74004) according to manufacturer's instructions. Library was prepared using Illumina_TruSeq total RNA library Kit following manufacturer's protocol.