VLP_rep1
Mast cells were cultured in complete RPMI 1640 , and supplemented with 10% fatal bovine serum, 100 U/mL penicillin/streptomycin (Gibco), 10 ng/mL rIL-3 and 20 ng/mL rSCF.Mannose receptors were ihibited with inhibors.The libraries were purified with the AMPure beads and the quality of libraries was assessed with Qubit.