GSM7775158: MC38 cells, P2ry6, UDP, rep1; Mus musculus; ATAC-seq
After treatment, 2×105 cells from each sample were lysed using 50 μl of cold cell lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.5% NP40) ATAC-seq libraries were prepared using a commercial ATAC kit (Chromatin Profile Kit for Illumina, Novoprotein Inc., Tianjin, China) following the manufacturer's instructions.