GSM7593322: Hi-C_pig_spleen_r1; Sus scrofa; Hi-C
Hi-C experiments were performed following previous study (Lieberman-Aiden et al., 2009) with slight modifications. In brief, following cross-linking with 1% formaldehyde and nuclei extraction, the chromatin was digested with Dpn Ⅱ and labeled with biotinylated residue after the 5' overhangs were filled. Blunt-end ligation of crosslinked DNA fragments was performed to obtain circular molecules, and then the DNA product was purified and sonicated into DNA fragments. Hi-C library was constructed by capturing the labeled target DNA fragments with biotin labeling precipitation technique followed by size selection. Finally, the obtained Hi-C library was sequenced by BGI company as 150 bp paired-end reads on DNBSEQ platform.