Helichrysum retrorsum  var. subulatum target-enriched library (leaf tissue)
DNA was extracted from 10-30 mg silica dried leaf tissue or herbarium material using the E.N.Z.A SP Plant DNA Mini Kit (Omega Bio-Tek, Norcross, Georgia, U.S.A.). Libraries were pooled and enriched with the COS 1061 bait kit (Mandel et al. 2014) designed for the Asteraceae family. Unenriched libraries were mixed with target-enriched libaries in a ration of 60:40.