GSM8013352: shNC-2.IP; Homo sapiens; ChIP-Seq
RNA libraries  were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols. RNA is randomly fragmented into approximately 200 nt fragments. Protein A/G magnetic beads  Rotate and incubate with m5C antibody at room temperature for 1 hour to allow the antibody to bind to magnetic beads. Then, the RNA fragment was combined with the magnetic bead bound antibody  Incubate at 4 degrees of rotation for 4 hours to allow RNA to bind to antibodies. Elution of RNA/antibody complexes from magnetic beads using Protein K