GSM8323235: Group M, O2-05, 0-6psi, 7 day, D6, S54; Homo sapiens; RNA-Seq
Total RNA was extracted by RNeasy Plus mini kit (Qiagen, Valencia, CA, USA), RNA quality was evaluated by analysis of the RNA integrity number determined using a 2100 bioanalyzer (Agilent RNA 6000 Nano Kit, Agilent Technologies, Santa Clara, CA, USA). 0.5μg total RNA was used for library prep with TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions. Validation of the library preparations was performed on an Agilent Bioanalyzer using the high sensitivity DNA kit (5067-4626, Agilent Technologies).