GSM7429779: MCF7, rep2; Homo sapiens; RNA-Seq
Total RNA (10 mg) was harvested from MCF7 using TRI reagent (Ambion) for cDNA library preparation. Enrichment of mRNA by depletion of ribosomal RNA was performed using a RiboMinus transcriptome isolation kit (Invitrogen). RiboMinus RNA (1 mg) was then fragmented using RNase III for 10 min and cleaned up using a RiboMinus concentration module (Invitrogen).