TruSeq PCR-free WGS of isolate 2 of Schizosaccharomyces pombe suppressor of etd1-M9
Libraries for whole genome sequencing were prepared according to manufacturers instructions with the TruSeq DNA PCR-free kit (Illumina) starting from 2200 ng of good-quality genomic DNA. Libraries were subsequently loaded at 8pM on a MiSeq v3 flow cells (Illumina) and sequenced according to manufacturer instructions, yielding pairs of 300nt reads (PE300). The mean output was 2 mio reads per sample, corresponding to a coverage of > 50x. Reads were trimmed of their adapters with bcl2fastq v2.18 and quality-controlled with fastQC v0.11.5.