GSM7676614: replicate2, ASCs_EWAT; Mus musculus; RNA-Seq
The mulitiple white adipose tissues were obtained, then cut into small pieces (~1mm) and digested with 1 mg/ml Type II collagenase (Sigma-Aldrich, MO) and 1% bovine serum albumin (Gibco, CA) at 37°C for 45 min with constant rocking. The cell mixture was filtered through a 70 μm filter into a single-cell suspension, followed by centrifugation at 500 g for 5 min, and stromal vascular cells were treated with a red cell lysis buffer. Appropriate cell suspensions were then hybridized with fluorescent dye-conjugated antibodies against the following mouse antigens. RNA libraries for RNA-seq were prepared following manufacture's protocols.