GSM7142283: Severe-February-rep 2; Citrus sinensis; RNA-Seq
Total RNA was extracted from 100 mg of the ground bud tissues using the RNeasy Plant Mini Kit (Qiagen, Valencia, CA). RNA quality and quantity were determined using a spectrophotometer and denaturing formaldehyde 1.2% agarose gels, respectively. RNA libraries were prepared for sequencing using the Illumina TruSeq 3000 mRNA protocol.