Nanopore sequencing of genomic DNA from the Drosophila melanogaster BG3 cell line
High molecular weight (HMW) DNA was extracted from ~2.5 million BG3 cells using the NEB Monarch HMW genomic DNA extraction kit for Cells and Blood. The Oxford Nanopore Technologies (ONT) library prep kit SQK-LSK114 was used to prepare a sequencing library from the extracted DNA, which was run on a single R10.1.4 flow cell using the MinION sequencing instrument. Basecalling was performed using Guppy version 6.4.8 with the following model: dna_r10.4.1_e8.2_260bps_hac@v3.5.2.