GSM7729139: MP46,FHT1015,1h,PRO-seq,Rep2; Homo sapiens; OTHER
MP46 uveal melanoma cells were treated with DMSO or 100 nM FHT-1015. Cells were chilled on ice, washed twice with ice-cold PBS and nuclei were isolated. Nuclear run-on reactions were performed in isolated nuclei by Arpeggio Biosciences. Following RNA extraction with Trizol, libraries were prepared using NEB Next Multiple Small RNA Library kit.  Libraries were sequenced on the Illumina NextSeq 550.