GSM8013012: HCT74_5; Homo sapiens; RNA-Seq
RNA was harvested using Trizol reagent. RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked with Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA). Excess DNA was removed by DNase I treatment (Qiagen) and RNA hydrolysis was protected with RNAsin (Promega). RNA libraries were prepared for sequencing using standard Illumina protocols