rhizosphere metatranscriptome from rapeseed at rosette growth stage without subsoil added to pot, sample no. 11
Rhizosphere soil was immediately snap frozen with liquid nitrogen and stored at -80C afterwards. Isolation of the RNA was performed using approximately 1.5 g  of soil with the RNeasy Power Soil Total RNA kit (Quiagen) and ROTI Phenol/Chloroform/Isoamylalcohol (25:24:1) (Carl Roth Scientific). RNA was purified with the RNA Clean & Concentrator-5 (Zymo Research) kit with in-column DNase digest. The purified RNA was stored at -80C before shipment on dry ice to Eurofins Genomics (Konstanz, Germany), where further processing of the samples was performed. rRNA of the samples was depleted before random cDNA synthesis and library preparation.