GSM7099008: smn2_nvs_lib3_rep3_in (MPSA); Homo sapiens; OTHER
Total RNA was extracted by Trizol following the manufacturer's protocol. Plasmids containing barcode-association were extracted by MaxiPrep using PureLink HiPure Plasmid filter Maxiprep kit (ThermoFisher). Then a fraction of them were applied for Illumina adapter ligation and pair-end sequencing A second round of amplification of the inclusion and total products added Illumina-compatible ends for sequencing