GSM7099515: #20 adjancent normal tissue; Homo sapiens; miRNA-Seq
Total RNA was extracted from the frozen tissues using the TIANamp DNA/RNA Isolation Kit (TIANGEN) and treated with RNase-free DNase I (Ambion) for 30 minutes at 37°C to remove contaminating DNA. RNA (1µg) from the tumor and paired adjacent tissues of 32 HCC patients were used to generate a miRNA sequencing library. RNA segments (18–30nt) were separated and recovered using a PAGE gel. The miRNA DNBs were loaded onto patterned nanoarrays and the 50 bp single-end reads were read using the BGISEQ-500 platform at Beijing Genomics Institute (BGI; Shenzhen, China).