GSM7675965: MOE_METATAC_210315-P7-Plate17; Mus musculus; ATAC-seq
The main olfactory epithelium was dissected and minced to small pieces, then was dissociated into single cell suspension with the Papain Dissociation System (Worthington Biochemical, Cat. #LK003150) at 37°C for 15 min, during incubation, titration every 5 min with wide-bore pipette tip according to previously described2. Then filtered with 30 μm strainer (MACS), then wash twice with ice-cold 1 x PBS. 10X Genomics scRNA-seq and METATAC scATAC-seq