GSM7678244: E1E2_An_linear; synthetic construct; RNA-Seq
These samples were resolved by agarose gel and the lower band in each sample containing circular RNA was gel extracted. Gel extracted T4 and An PIE N60 RNA was used for first-strand DNA synthesis using SuperScript III reverse transcriptase (Thermo Fisher) with primer N60 junction R1 according to manufacturer's instructions and this cDNA was subsequently was used as template for PCR amplification with primers N60 junction F and R1. For gel extracted Sh and Po PIE N60 RNA, primer N60 junction R2 was used to synthesize first-strand cDNA with SuperScript III reverse transcriptase (Thermo Fisher) and this cDNA was subsequently used as template for PCR amplification with primers N60 junction F and R2.