GSM7312029: CLIPseq_DDX10_rep1; Mus musculus; OTHER
The RNAs were subjected to NuPAGE Bis-Tris gel separation and transferred to nitrocellulose membrane, and the biotin-labeled RNA-protein complexes were identified by chemiluminescent nucleic acid detection module kit. Finally, the RNAs were purified through proteinase K treatment and overnight precipitation. The RNAs were ligated with 5' RNA adaptor with T4 RNA Ligase1. Then the RNAs were reverse transcribed by Superscript III with RT primer and amplified for 15-18 cycles with Phusion High-Fidelity DNA Polymerase. Library index sequences were introduced by PCR with index primers for 8-10 cycles.