GSM7311918: GRKO_MR-2B12_Ald_RNA_Rep1; Mus musculus; RNA-Seq
Cells fixed in 1% Formaldehyde. Lysed, Sonicated, and ChIP'd with anti-GFP (10 μg, Abcam #AB290) or anti-GR (10ug Santa Cruz # sc-393232x. Total RNA was collected using Pure-Link RNA Mini kit (Thermo). ChIP-Seq libraries were made by TruSeq ChIP Library Prep Kit (IP-202-1012). RNA-Seq libraries were made with TruSeq Stranded Total RNA Library Prep. ChIP-seq was performed with Next-Seq (76bp single-end reads) and RNA-Seq was performed with NOVA-Seq (150bp stranded reads).