GSM8013386: R23G12-Gal4 Drgx knockdown1; Drosophila melanogaster; RNA-Seq
Brains were dissected in cold Drosophila Schneider medium. Brains were dissociated with Papain and Liberase into a single cell solution. T4/T5 neurons were expressing GFP and  sorted out from the single cell solution with a FacsAria Ilu sorter into TRL Plus Buffer of the RNeasy Plus Micro Kit. Total RNA was extracted using the RNeasy Plus Micro kit according to the manufacturer's protocol. libraries were prepared with SMART-Seq v4