GSM7316752: day 4, Padi4-flox, replicate 1; Mus musculus; ATAC-seq
Nuclei were extracted by treating the isolated neurons with lysis buffer (IGEPAL CA-630 0.1 %, Tris-HCl 10 mM, NaCl 10 mM and MgCl2 3 mM) and treated with Tn5 transposase (Illumina, Nextera DNA Library Preparation Kit) for fragmentation and tagmentation of genome DNA. The DNA fragments were PCR amplified with KAPA HiFi HS ReadyMix (KAPA BIOSYSTEMS). Post-amplification libraries were size-selected at 200-1000 bp in length using AMPure XP beads (BECKMAN).