GSM7428078: F2_Mtrr+_+_PN_meDIP_rep_7; Mus musculus; MeDIP-Seq
Genomic DNA was isolated and purified using the QIAamp Fast DNA Tissue Kit(Qiagen) Genomic DNA was sonicated to yield 150–600 bp fragments, and adaptors for paired-end sequencing (Illumina) were ligated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs). Immunoprecipitations were carried out using 500ng DNA per sample, 1.25μg anti-5mC antibody (Eurogentec Cat# BI-MECY-0100, RRID:AB_2616058) and 10μL Dynabeads coupled with M-280 sheep anti-mouse antibody (Invitrogen). Pulled down DNA was amplified for 12 cycles with adapter-specific indexed primers. Final clean-up and size selection was carried out with AMPure-XP SPRI beads (Beckman Coulter).