RNA-Seq of cotton leaves
After passing the sample testing, the library is constructed, and the main process is as follows:(1) Enriching eukaryotic mRNA with magnetic beads containing Oligo (dT);(2) Add Fragmentation Buffer to randomly interrupt mRNA;(3) Using mRNA as a template, the first cDNA strand was synthesized using random hexamers, followed by adding buffer, dNTPs, RNase H, and DNA polymerase I to synthesize the second cDNA strand. The cDNA was purified using AMPure XP beads;(4) The purified double stranded cDNA is then subjected to end repair, A-tailed addition, and connected to sequencing connectors, followed by fragment size selection using AMPure XP beans;(5) Finally, a cDNA library was obtained through PCR enrichment