GSM8322246: HCC827_Er, ChIP; Homo sapiens; ChIP-Seq
The HCC827 and HCC827_Er cells were crosslinked with formaldehyde treatment and chromatin fragmented to 200 to 300 bp by sonication. Chromatin from 2 × 107 cells was used for each ChIP experiment, which yielded approximately 200 ng of DNA. The ChIP DNA was purified by QIAquick PCR Purification Kit (Cat. 28104; QIAGEN) following the manufacturer's protocol.  The ChIP DNA library preparation was performed by using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (Cat. E7645S; NEB).  Paired-end sequencing was performed with Illumina NovaSeq platform.