PacBio HiFi WGS of Brassica napus
About 100 g of leaf material of eight individual plants was ground up and homogenized under liquid N2, using a mortar and pestle. Plant material was then aliquoted on dry ice. DNA extraction was performed using the SOP of Lab 3. HMW DNA (> 40 kb/fragments) was extracted. The sequencing target was set to 15 kb CCS reads, reaching at least a 25x HiFi coverage of the B. napus genome (1 Gb).