d27-1 line 16_R1
The plants were grown on half-strengthGamborgs B5 medium for 5 weeks. For uniform tissue sampling,a 6 mm diameter disc encapsulating GC3 and GC4 was harvested,weighing approximately 160 mg per biological replicate (with threebiological replicates per genotype). Total RNA was extracted usingthe Spectrum Plant Total RNA Kit (Sigma Aldrich) following themanufacturers instructions. RNA-sequencing library constructionand sequencing was performed by Beijing Genomics Institute(Shenzhen, China) using DNBSEQ Eukaryotic Strand-specificmRNA library preparation and the DNBseq platform.