GSM7594681: normal,rep2; Homo sapiens; miRNA-Seq
Total RNA was extracted using using Trizol reagent following the manufacturer's protocol. RNA purity was checked using the kaiaoK5500Spectrophotometer (Kaiao,Beijing, China). RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Total RNA was separated by 15% agarose gels to extract the small RNA (18-30 nt). After precipitated by ethanol and centrifugal enrichment of small RNA sample, the library was prepared according to the method and process of Small RNA Sample Preparation Kit (Illumina, RS-200-0048). The main contents are as follows: 1) Connecting the 3' adaptor to the separated small RNA. 2) Connecting the 5' adaptor to the separated small RNA. 3) RT－PCR. 4) Recycling strips of 145-160bp (22-30nt RNA).