16S rRNA-Seq of Coturnix japonica: adult female cecum
DNA from cecum contents was extracted according to the standard protocol of ISOSPIN Fecal DNA (Kobayashi Ltd., Tokyo, Japan). Extracted DNA was quantified for DNA concentration using a Qubit3.0 Fluorometer (Q33216; Thermo Fisher Scientific, Waltham, MA, USA) and Qubit dsDNA HS Assay Kit (Q32851; Thermo Fisher Scientific). DNA samples were submitted to the amplicon sequencing (16S rRNA analysis) service of Bioengineering Lab. Co. (Kanagawa, Japan) and sequenced on an Illumina Miseq paired-end 2300 bp.