GSM6434372: Moricandia moricandioides, T1, control, biol rep M6c; Moricandia moricandioides; RNA-Seq
Total RNA was isolated using the RNeasy Plant Mini Kit (Qiagen, Germany) according to the manufacturer's protocol and digested with DNase I using on-column Qiagen DNase, as per manufacturer's instructions. The intactness of the RNA extracted was verified by 1% agarose-TBE gel electrophoresis total RNA concentration and purity was verified through NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA) measurements. After quality control on a Bioanalyzer 2100 (Agilent, Santa Clara, USA), RNASeq was performed on an Illumina HiSeq2500 platform at the Heinrich Heine University (Biologisch-Medizinisches Forschungszentrum.