GSM7098162: PolyA_RNAseq_Patient_3_PB_Cycle2_Day1; Homo sapiens; RNA-Seq
RNA was extracted using the Qiagen RNeasy extraction kit, according to the manufacturer's instructions. A minimum of 500 ng of high-quality RNA (as determined by Agilent Bioanalyzer) per replicate was used as input for library preparation. Poly(A)-selected, strand-specific (dUTP method) Illumina libraries were prepared by the Integrated Genomics Operation (IGO) at Memorial Sloan Kettering Cancer Center (MSKCC) with a modified TruSeq protocol. Libraries were sequenced on the Illumina HiSeq 2000 to obtain ∼100M 2x101 bp paired-end reads per sample.