Ceratomia igualana: adult abdomen
Library prep followed the protocols of Lemmon et al. (2012),  Breinholt et al. (2018), and Hamilton et al. (2019). Extracted genomic DNA was fragmented to ~250 bp inserts, then sample-specific barcodes and Illumina sequencing adapters were ligated to the inserts. Barcoded inserts were pooled and the Agilent Custom SureSelect BOM1 probe kit was used to isolate 571 loci. Enriched libraries were sequenced as paired-end 100 bp on an Illumina HiSeq 2500.