Illumina HiSeq 2500 paired end sequencing; RNA-seq of Arabidopsis thaliana seedlings to investigate SMB inducible misexpression
RNA-seq of Arabidopsis thaliana seedlings to investigate SMB inducible misexpression
collection of Arabidopsis seedlings grown on ½ MS agar plates 6 hours after treatment with DMSO (1:1000, mock) or dexamethasone (10μM). Col-0 wild type plants or SMB-GR misexpressing plants ; whole seedlings were harvested with DMSO (mock) or dexamethasone (10μM) to induce SMB-GR movement to the nucleus total RNA extraction with a Spectrum Plant Total RNA Kit Library preparation was done using the TruSeq stranded RNA Sample Preparation Kit v2 (Illumina).