GSM7428185: vag LC bacteria supernatants r1; Homo sapiens; ATAC-seq
Nuclei were extracted by centrifuging the cells at 500 × g for 10 minutes at 4°C, washing with cold 1x PBS and centrifuging again.  50,000 cells were lysed using cold ATAC lysis + Digitonin (2%). Immediately after lysis, nuclei were spun at 500 × g for 10 minutes ATAC-seq libraries were generated using the ATAC-seq Kit from Diagenode (Diagenode, A Hologic Company) according to manufacturer instructions