GSM7140662: CSR_PQRXLF-2; Mus musculus; OTHER
Genomic DNA was extracted from treated B cells on day 4. Libraries were prepared according to the protocol in (Hu J, et al. Nat Protoc 11:853–871 (2016)). Linear amplifications were performed using Sμ-specific biotin primer (/5Bios/CAGACCTGGGAATGTATGGT) and followed by nesting PCR with (5′-CACACAAAGACTCTGGACCTC-3′). AflII was used to remove germline sequence. High-throughput genome translocation sequencing (HTGTS). Sequenced with MiSeq 500v.