Illumina HiSeq X Ten sequencing of Bathysaurus mollis: Adult Hermaphrodite muscle
Genomic DNA was extracted from muscle tissue samples. The DNA was cross-linked with formaldehyde to preserve chromatin interactions. Cross-linked DNA was digested with a restriction enzyme, and the resulting fragments were ligated to form chimeric junctions. These chimeric DNA fragments were purified and sheared to an average size of ~350 bp using a Covaris ultrasonicator. Hi-C libraries were constructed using the Illumina TruSeq DNA Library Prep Kit. Libraries were indexed, pooled, and sequenced on an Illumina HiSeq X Ten platform to generate 2 x 150 bp paired-end reads.``