GSM7426958: RPTC_384_NONE000_3_0_N; Homo sapiens; RNA-Seq
Cell lysates were prepared from OrganoPlate® 3-lane 40 devices through injection lysis buffer into the RPTEC channel. Following 10 minutes of incubation at room temperature, the cell lysate was flushed out by addition lysis buffer. For 384 well plates, media was aspirated and lysis buffer was added and cells were incubated for 10 minutes at room temperature after which the lysates were collected from each well. All lysates were stored at −80 °C until further processing. The Templated Oligonucleotide Sequencing Assay (TempO-Seq™, BioSpyder Technologies, Carlsbad, CA) was used as an RNA sequencing technology of choice. Detailed protocols for TempO-seq are provided by the manufacturer and were previously reported  detailed elsewhere (Grimm et al., 2016; House et al., 2022) TempO-seq libraries were prepared using the S1500+ targeted transcriptome panel (Mav et al., 2018) consisting of 2,982 transcripts according to the manufacturer's instructions.