GSM7946898: Ctrl_vehicle_V2507; Mus musculus; RNA-Seq
Approximately 100 mg of mouse heart tissue were lysed with TRI Reagent (Zymo Research) and frozen at -80 °C to ensure complete cell lysis. Nuclei isolation was performed as per instructions from 10xGenomics "Nuclei Isolation from Cell Suspensions & Tissues for Single Cell RNA Sequencing" protocol (CG000124, Rev F) to reach the target concentration of 700-1,200 nuclei/µl. The suspension was processed using 10X Genomics Single Cell protocol (CG00053, Rev D). We constructed 3' single cell gene expression libraries (Next GEM v3.1) using the 10x Genomics Chromium system. Each library was sequenced with Illumina NovaSeq (PE150). snRNA-seq