GSM6738964: A08_DMSO_1, eHAP FNLS SUGP1 E554K, DMSO, replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNAqueousTM-96 Total RNA Isolation Kit (ThermoFisher #AM1920) according to the manufacturer's protocol. Poly(A)-enriched RNA was obtained with the poly(A) RNA Selection Kit V1.5 (Lexogen #157.96) and RNA-seq libraries were generated using the CORALL Total RNA-Seq Library Prep Kit (Lexogen #117.96). The libraries were then sequenced using paired-end 150 bp reads with 60 million reads per sample on Nova Seq (S4).