Pool-seq of Lower C&B Physella johnsoni
DNA isolated from 40 individual snails using OMEGA Bio-Tek E.Z.N.A. Mollusc DNA Kit. DNA was quantified a minimum of twice using a Qubit and equal nanogram (ng) amounts from each individual were pooled for each population, quantified as above and each pool was diluted to a final concentration of 3 to 6 ng/uL. Libraries were prepared using a shotgun approach with PCR with Illuminia TrueSeq LT adaptors and were sequenced over four lanes on Illumina HiSeq X using paired-end reads of 150 base pairs (bp) by Gnome Qubec Innovation Centre, Montral, Qubec, Canada.