GSM7099083: T47D_withoutE2_SHANK2_rep2; Homo sapiens; OTHER
Rapid lysis buffer + 10ug/ml Proteinase K, at 56°C, overnight See Supplemental Experimental procedures from Chiarle et al.  Cell 2011, paragraph Generation of HTGTS libraries by adapter-PCR Gennomic DNAs were extracte with Rapid lysis buffer containing 20 ug/ml of proteinase K overnight at 56°C. Library was prepaired with the HTGTS methods previously described (Chiarle at al., Cell, 2011_PMID:21962511; Genome-wide Translocation Sequencing Reveals Mechanisms of Chromosome Breaks and Rearrangements in B Cells) We induced DNA DSBs in intron 10 of SHANK2 or in intron 1 of RARA. We used linker forward primer and specific target reverse primers to capture functional fusions.