WGS of experimentally evolved Pseudomonas fluorescences (SBW25) fleQntrC inducible EBP expression systems
To identify motility rescuing mutations, genomic DNA was extracted from motile strains and their ancestral strain using the Thermo Scientific GeneJET Genomic DNA Purification Kit. Genomic DNA was quality checked using BR dsDNA Qubit spectrophotometry to determine concentration and nanodrop spectrophotometry to determine purity. Illumina NextSeq 2000 sequencing was provided by Seqcenter (Pittsburgh, PA, USA), with a minimum 30x coverage.