GSM7985975: hmSpVO_rep2; Homo sapiens; RNA-Seq
Total RNA was extracted using the FastPure Cell/Tissue Total RNA Isolation Kit according to the manufacture's protocol. Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific) was used to determine the concentration and evaluate the purity of RNA samples. Agilent 2100 Bioanalyzer and 2100 RNA nano 6000 assay kit (Agilent Technologies) were used to evaluate the integrity of RNA samples. For samples of C3,C4,C6 and hdMO_rep1,the libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and were sequenced on Illumina HiSeq X Ten platform. For samples of hdMO_rep2 and hdMO_ventr,, the mRNA was enriched by TIANSeq mRNA Capture Kit (TIANGEN) and the libraries was constructed by TIANSeq Fast RNA Library Kit (Illumina). The libraries were sequenced on Illumina NovaSeq 6000.