GSM7427733: BRM-GFP_brm-1-DMSO-IP-bio2; Arabidopsis thaliana; ChIP-Seq
Lysates were clarified from sonicated protein-DNA complexes were isolated with GFP antibody. Two ng of ChIP DNA immuoprecipitated by anti-GFP antibody was used for ChIP-seq library construction. Blunting end, adapter ligation and amplification were carried out using Illumina Genomic DNA Sample Prep Kits according to the manufacturer's protocol. Illumina Novaseq was used for high-throughput sequencing of the ChIP-seq library.