GSM7787510: HEK293T (B9 clone + KRAB), reactivated, H3K27me3, CUT&RUN, rep1; Homo sapiens; OTHER
Cells were permeabilized by 0.025% digitonin followed by incubation with antibody and Mnase excision using CUTANA ChIC/CUT&RUN Kit (14-1048, EpiCypher) Library was prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, New England Biolabs) for H3K9me3, H3K4me3 (positive control), IgG (negative control) or CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1 (14-1001, EpiCypher) for other modifications. Library fragment sizes were checked with Agilent 2100 Bioanalyzer.