GSM7139985: WTCHG_70085080_D0_MenACWY_D0_only; Homo sapiens; RNA-Seq
Peripheral blood (up to 2.5ml) was collected into a PAXgeneTM RNA tube at vaccination (D0), SARS-CoV-2 diagnosis (CT) and 7 days post diagnosis (CT+7). Total RNA was extracted using the PAXgene blood RNA kit (PreAnalytiX, Switzerland) on the QIAsymphony SP (Qiagen) with the PAX_RNA V5 protocol. Eluted RNA was quantified using the Agilent RNA ScreenTape assay on the 4200 TapeStation System (Agilent). RNA was ribodepleted and globin depleted using Ribo-ZeroTM Gold rRNA removal Kit (Illumina, USA). RNA was converted to cDNA, second-strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated and prior to amplification, samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and quality controlled before 150bp paired-end sequencing (NovaSeq6000).