GSM8322257: SOD1_2, SnRNA-seq; Mus musculus; RNA-Seq
After the cell was suspended, the cell was filtered and collected by 20um cell sieve and counted. Gel Beads containing barcode information bind to a mixture of nuclei and enzymes and are then coated with droplets of oil surfactants located In a microfluidic system to form GEMs (Gel Beads in-emulsions). GEMs flow into a reservoir and are collected, the gel beads dissolve to release the Barcode sequence, reverse transcrib the cDNA fragment, and label the sample. The gel beads were broken and the oil droplets were broken, and PCR amplification was performed using cDNA as template. The standard sequencing library was constructed by mixing all GEMs products.