GSM7729733: C-2-2; Mus musculus; RNA-Seq
RNA was sequenced from eggs or embryos from the 1-cell to the blastocyst stage. Four biological replicates at each stage for each group were sequenced. Each biological replicate consisted of five eggs or embryos. Following collection as described, eggs and embryos were washed in sterile PBS and snap-frozen on dry ice. RNA was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio, Japan), and cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter). RNA quality and yield were measured using the Agilent 2100 Bioanalyzer (Agilent Technologies). Library preparation was carried out on 100-150 pg amplified cDNA using the Illumina DNA Prep (Illumina, San Diego, CA). Sequencing was performed on a NovaSeq 6000 Sequencing System (Illumina).