GSM7593313: Multiplexed-Fibroblast Line H01 to H05, scRNAseq; Homo sapiens; RNA-Seq
Treated fibroblasts were dissociated using 0.05% Trypsin-EDTA and resuspended in cDMEM. Cells are labelled with CMO barcodes and sorted using PI stain and resuspended in loading buffer (PBS + 0.1% BSA). Finally, cells are pooled and loaded for single cell 3' HT V3.1. Library was performed according to the manufacter's instructions (single cell 3' HT V3.1, dual-index, multiplex barcode)