GSM7311788: Hs578T_8 weeks_shPRRX1_3_DOX; Homo sapiens; RNA-Seq
Total RNA was extracted using the Qiagen ALL Prep kit (cat#80204) RNA-seq libraries were prepared using Illumina TruSeq Stranded mRNA sample preparation kits from 500 ng of purified total RNA according to the manufacturer's protocol. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to the manufacturer's protocols.