GSM7987383: PJS1-2_3; Homo sapiens; RNA-Seq
RNeasy Mini Kit The Illumina Stranded mRNA Prep Kit was used to prepare sequencing libraries from 100 ng of high-quality RNA samples (all samples had RIN>8). The kit procedure was performed without modifications. This procedure includes mRNA purification and fragmentation, cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR amplification and validation.