GSM6796784: ATAC-Seq ST-rep2; Sus scrofa; ATAC-seq
Living cells with a 10 minutes' incubation at room temperature ATAC-seq was performed as previously described (Corces et al. 2017) with some modifications. Briefly, cell pellets were resuspended in lysis buffer, and the pellet was resuspended with resuspension buffer. The DNA was further purified with Sera-Mag carboxylate-modified magnetic beads and eluted with H2O with 0.1% Tween-20. The purified transposed DNA was amplified, and the DNA libraries were size-selected with Sera-Mag carboxylate-modified magnetic beads and sequenced on the NovaSeq 6000 platform.