GSM7677236: ern0160-deleted Aus0004 mutant - sample 2 [d0160-2]; Enterococcus faecium Aus0004; RNA-Seq
Total RNA was extracted from using the phenol/chloroform method. Residual chromosomal DNA was removed by treating samples with the TURBO DNA-free kit (Life Technologies). The strand-specific library preparation was performed using the Ovation Universal Prokaryotic RNA-Seq kit (Nugen) with custom depletion (Anydeplete rRNA design Enterococcus faecium) and the samples were sequenced using the Illumina NextSeq500 instrument (multiplex protocol, single-end 75-bp reads).