GSM7317570: RESCUE tumor_921; Homo sapiens; RNA-Seq
To extract total RNA from the tumor samples, the tissue was first lysed and homogenized by dual centrifugation at 2500 rpm, -5 °C for 4 minutes using the ZentriMix 380R (Hettich) and RNA was purified using the GeneElute™ Mammalian Total RNA Midiprep Kit (Sigma Aldrich) following the manufacturer's instructions, including the in-column DNAse digestion step. Libraries were prepared with the Illumina TruSeq Stranded Total RNA library kit, including rRNA depletion.