GSM7594379: ONP, day 30 post microbeads injection, rep1; Mus musculus; RNA-Seq
HA-tagged risobosomes were isolated using anti-HA antibody and magnetic beads separation. RNA was extracted using the RNeasy Plus Micro Kit (Qiagen). 3.95-37.4 ng of RNA were used to make libraries. mRNA was extracted with oligo-dT beads, capturing poly(A) tails, and cDNA libraries were made with the Smart-Seq HT Ultra-low input RNA kit (Takara Biosciences)