GSM7988647: CD8+ T Control 2 (ATAC-seq); Mus musculus; ATAC-seq
Tagmented DNA was isolated using 2x SPRI Beads (Beckman Coulter) and amplified through PCR. Fragments smaller than 600 bp were isolated via negative size selection using 0.65x SPRI Beads (Beckman Coulter) and purified using 1.8x SPRI Beads (BeckmanCoulter). Libraries were created following a modified protocol from Buenrostro et al. In summary, 50,000 FACS-purified CD8+ T cells per subset were washed with PBS and suspended in 50 μl of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM MgCl2, 0.1%IPEGALCA-630).