Metabarcoding of eukaryotic 18S from eDNA extracted from extraction kit blank.
Eukaryotic 18S rRNA was amplified using the primers Euk_1391f (TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTACACACCGCCCGTC) and EukBr (GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGATCCTTCTGCAGGTTCACCTAC), with Nextera compatible adapter tails on the five prime ends.