RNA-Seq of Homo sapiens
Samples were analyzed using the CancerDetect test. The methods for this test have been previously published (Toma, R. et al. A clinically validated human saliva metatranscriptomic test for global systems biology studies. Biotechniques 74, 3144 (2023).). Briefly, the method includes DNase treatment, non-informative RNA depletion, cDNA synthesis, size selection, and limited cycles of PCR for adding dual unique barcodes to each sample.