GSM7506328: UUO Sham KO 1; Mus musculus; RNA-Seq
Total RNA was isolated from mouse tissue using an RNA mini kit (Qiagen). RNA purity and integrity were confirmed by Agilent Bioanalyzer. Sequencing libraries were prepared from 100-500ng total RNA using TrueSeq RNA sample preparation kit v2 (Illumina). Broadly speaking, mRNA is purified, fragmented, and used for first and second strand cDNA synthesis, followed by adenylation of the 3 '-end. The samples were connected to a unique adapter and amplified by PCR. The libraries were validated, standardized, and sequenced using the 2100 BioAnalyzer(Agilent). Three bioreplicated RNA-seq libraries were sequenced on Illumina HiSeq 2000 using barcode multiplexing and a read length of 100 bp for each condition, four bioreplicates were performed for each condition.