GSM7593856: Bulk, DRG, Uninjured; Mus musculus; RNA-Seq
For total RNA sequencing, freshly collected DRGs were lysed, and their RNA was extracted using RNeasy mini kit(Qiagen, 74104). For HA-IP sequencing, lysate from the freshly collected DRGs of RiboTag mice were immunoprecipitated with an anti-HA antibody, and the RNA from the immunoprecipitated product was extracted using the same kit. Both libraries for total RNA sequencing and HA-IP sequencing were prepared following the instrcutions from the manufacture. (Total RNA sequencing: direct RNA sequencing kit(SQK-RNA002), HA-IP sequencing: cDNA-PCR barcoding kit(SQK-PCB109))