GSM8061383: AMO-IIIF9-2h-melphalan-20-biol rep 1; Homo sapiens; RNA-Seq
Accoding to protocol for RNeasy Plus Mini Kit (Qiagen) DNA libraries suitable for sequencing were prepared from 500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer's instructions (½ volume). After 15 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~300 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips. Sequencing of pooled libraries, spiked with 1% PhiX control library, was performed at 31-46 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina).