Deep sequencing of BlaC
Samples for Illumina sequencing were prepared by PCR using plasmid libraries as templates. Amplicons were barcoded using universal tail sequences and sequenced on Illumina NovaSeq 6000 sequencer. Samples were sequenced paired-end 150-bp using 300 cycle v1.5 sequencing reagents. FASTQ files were generated using bcl2fastq v2.20. Target region offset is only for forward reads (F).