RIP-seq of CN725425-HA in mouse testis, CN725425-HA , rep1
Testis lysate was subjected to IP using anti-HA antibody (CST #3724). Total RNA was then isolated from beads using Trizol reagent (Thermo Fisher). Strand-specific libraries were constructed using the TruSeq RNA sample preparation kit (Illumina, San Diego, CA, USA).