GSM7058826: S.sanguinis + P.gingivalis in Glucose, anoxic conditions replicate 3; Streptococcus sanguinis; RNA-Seq
RNA was extracted from whole cells using a Trizol-based protocol, genomic DNA was digested using TURBO DNaseI according to manufacters protocol and samples were cleaned using Qiagen RNeasy kit. 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Illumina TruSeq Stranded with rRNA and Globin Depletion kit following manufacturer's protocols.