GSM7986544: E12.5_control_scRNAseq; Mus musculus; OTHER
Histone modifications in this study were identified using the Cleavage Under Targets and Tagmentation (CUT&Tag) technique, following the protocol published by the Henikoff lab. For both wild-type (WT) and HDAC1/2 conditional knockout (cKO) cerebral cortices, three biological replicates were prepared. We employed the Rabbit anti-H3K27ac antibody (Active Motif 39136) for specific targeting. The Tn5-tagmented DNA fragments were then amplified using the 2x CAM Mix, a uniquely barcoded i5 primer, and a uniquely barcoded i7 primer, undergoing 12 amplification cycles. Libraries were quantified on a Qubit 4 fluorometer (Thermo Fisher Scientific Q33226). We performed size-selection on the libraries, isolating DNA fragments exceeding 300 base pairs using VAHTS DNA Clean Beads (Vazyme N411). Finally, the pooled libraries were sequenced in a paired-end mode using the Illumina NovaSeq 6000 platform.