GSM7787477: Vgat-Cre_410_Rep_6_L2; Mus musculus; RNA-Seq
N2a cells lifted in 250uL PBS and 750µL Trizol, followed by addition of 200uL chloroform and cleanup of the aqueous phase using the Zymo Clean and Concentrator 5 kit per manufacturer instructions. Vglut-cre and Vgat-cre brains were harvested at P21 and homogenized. Lysates were clarified and mixed 3:1 with Trizol, followed by addition of 200uL chloroform and cleanup of the aqueous phase using the Zymo Clean and Concentrator 5 kit per manufacturer instructions. Reporter-specific cDNA synthesis using a reverse-complementary primer, followed by PCR, digestion, adapter ligation, and index PCR. DNA from viral prep/cells was prepared simultaneously, starting with the first PCR step.