GSM7988164: cx3cr1-control_brain_O4+ cell; Mus musculus; RNA-Seq
Total RNA was extracted utilizing the RNeasy Mini Kit (Qiagen; Cat# 74104) and its concentration was determined using a Nanodrop 2000 spectrophotometer and a Qubit fluorometer (both from ThermoFisher Scientific). The quality of the extracted RNA was evaluated using a 2100 Bioanalyzer (Agilent). For O4+ cell-specific transcriptomics, library preparation was performed using the SMART-Seq Stranded Kit (Takara Bio; Cat# 634442) following the manufacturer's protocols. Sequencing was carried out on a NextSeq 500 system (Illumina) with the NextSeq 500/550 High Output Kit v2.5 (Illumina; Cat#20024906).