GSM7098855: PBP1 knockout cells in YPD media, biological replicate 2; Saccharomyces cerevisiae; RNA-Seq
RNA was extracted using the Masterpure Yeast RNA extraction kit (Lucigen). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. This was followed by fragmentation, first strand cDNA synthesis using random hexamer primers, and second strand cDNA synthesis. End repair, A-tailing, adapter ligation, size selection, amplification, and purification were then performed to generate the library, which was subsequently checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Libraries were sequenced on Illumina platform.