GSM8061442: mESC-Igf2_Igf2_inverted~dEnh~dCTCFinICR-BS24924A; Mus musculus; OTHER
Cells were dissociated using TryPE-Select, spun-down and media aspirate. Pellets were either processed immediately or stored at -80 deg. Genomic DNA was extracted using the DNeasy Blood & Tissue kit (QIAGEN 69506). Payload and Targeted sequencing (Capture-seq); Double-stranded DNA libraries were prepared from sheared genomic DNA. DNA fragments were end-repaired, A-tailed, and Illumina-compatible adapters were ligated to DNA ends.