GSM7729310: Reactivated, scRNAseq; Mus musculus; RNA-Seq
Single cell suspension was obtained by dissociated embryos for 10-15min at 37 °C with TrypLE express enzyme. The cells were then loaded on to a microwell cartridge of BD Rhapsody Express system (BD Biosciences). Single-cell whole transcriptome analysis libraries were prepared according to the manufacturer's instructions using a BD Rhapsody WTA Reagent kit (BD Biosciences) and sequenced on the Illumina NextSeq 500 using the Mid Output Kit v2.5 (150 cycles, Illumina).