GSM6742610: IGF1exp-750ngIGF1-6h-OLA985-E14-M1-Emb3; Mus musculus; RNA-Seq
Tissue was stored in RNAlater (Qiagen, Düsseldorf, Germany) at −80°C for RNAseq. For RNAseq, each tooth was handled individually. Samples were homogenized using Precellys 24 homogenizer (Bertin instruments). RNA was extracted using guanidium thiocyanate-phenol-chloroform method and purified using RNeasy Plus micro kit (Qiagen GmbH) following manufacturer's protocol. The putiry of RNA was measured using Nanodrop microvolume spectrophotometer (Thermo Fisher Scientific). The complementary DNA (cDNA) libraries were prepared using Ovation Mouse RNAseq System (Tecan).