GSM8322063: CnT_H3K27me3_ASCL1KO_Wk10_Rep2; Homo sapiens; OTHER
CUT&TAG-Direct was performed with anti-H3K27me3 (CST 9733). Tagmented genomic DNA was extracted using 1%SDS/Proteinase K Released, tagmented DNA from ~50,000 starting cells was amplified using non-hot-start polymerase (NEB M0541S) using the following program: 58oC, 5 mins; 72oC, 5mins; 98oC 30s; (98oC 10s, 63oC 30s) x 12 cycles total, 72oC, 1min.