GSM8061435: Fibroblast Normoxic Co-Culture 3; Homo sapiens; RNA-Seq
Immediately following treatment, the co-culture region was cut to separate hypoxic and normoxic cells with a sterile razor blade and then 0.5 mL TriZOL was added to each of four regions (0% control, 0% co-culture, 10% control, 10% co-culture). TRIzol-chloroform separation followed by RNA purification using a Qiagen miRNA Mini Kit were used to isolate mRNA transcripts. xGen Broad-Range RNA Library Prep Kit