GSM7594478: Human_A549, 1 hour post infection, rep3; Homo sapiens; RNA-Seq
Three biological replicates were prepared by condition. Oligo(dT)-attached magnetic beads were used to purify mRNA, and cDNA was generated using random hexamer-primed reverse transcription synthesis. The product was validated using the Agilent Technologies 2100 bioanalyzer. Samples were processed and 50 base reads were generated on BGIseq500 platform (BGI-Shenzhen, China). The sequencing reads were filtered with SOAPnuke (v1.5.2) and mapped to a modified version of the reference human genome (GRCh38/hg38) containing also the viral genome HSV-1 genome (HSV-1 strain KOS, GenBank accession code JQ673480.1) using STAR (v2.5.2a).