GSM7677581: SK-HEP-1 S; Homo sapiens; ATAC-seq
50000 cells were centrifuged using 4℃,5 min and 500 g centrifuging parameters, and then the supernatant was removed. The cells were washed with cold PBS once. Using 4℃,5min and 500 g centrifuging parameters the supernatant was removed.The cells were suspended with cold lysis buffer. Using 4℃,10 min and 500 g centrifuging parameters the supernatant was removed. The transposing reaction system was configured with the Tn5 Transposase. The cell nucleus were suspended with the transposing reaction system, and the DNA were purified after incubating at 37℃ for 30 minutes. The PCR reaction system was configured with the purified DNA ,and then the PCR amplification reaction was performed. Purify amplified library using Qiagen MinElute PCR Purification Kit.