GSM7316530: HAP1 sgRNA3 + HDR temp passage 2 Rep 2 Tn5; Homo sapiens; OTHER
Total genomic DNA was isolated using the Macherey-Nagel NucleoSpin Tissue Mini DNA extraction kit. Tn5 transposase conjugated with Illumina/Nextera-compatible adapters was used to simultaneously fragment and ligate adapters onto genomic DNA. Resultant fragments were amplified with Illumina i5/i7 universal primers and unique dual index primers in a subsequent barcode PCR reaction. Tn5 transposase library preparation with Illumina/Nextera-compatible adapter overhangs and dual indices