GSM7595064: PBS, mouse 4; Mus musculus; RNA-Seq
Total RNA was isolated from mouse ileum then purified with RNeasy Plus universal kit (Qiagen, Hilden, Germany) following manufacturer's instructions. Illumina-compatible RNA-seq libraries were constructed using the NEBNext Ultra II RNA library prep with sample purification beads (Cat. No. E7775) and NEB Next Multiplex Oligos for Illumina (dual index primers set 1; Cat. No. E7600) according to the manufacturer's instructions.