GSM6600455: NKD1; Zea mays; OTHER
DNA was extracted from maize B73 14-day old seedlings. Five grams of tissue was ground in liquid nitrogen and the genomic DNA was extracted in a phenol/chloroform/IAA extract cocktail. The DNA pellet was resuspended and diluted in EB (10mM Tris-HCl pH 8.5). The EB diluted DNA was sheared to 200bp fragments using a Covaris S2 sonicator. DNA was purified using AmpureXP beads with a 2:1 bead to DNA ratio, and then end-repaired using an End-It kit (Lucigen) according to the manufacturer's recommendations. Samples were cleaned using a Qiaquick PCR purification kit (Qiagen), A-tailed using Klenow (3' to 5' exo-), cleaned with Qiaquick and ligated overnight with a truncated Illumina Y-adapter. Final libraries were purified using AMPureXP beads with a 1:1 bead to DNA ratio and eluted in 30ul of EB. DNA concentration was determined using the Qubit HS kit.