EU-RNA-Seq of HeLa: 20 minutes after labeling
HeLa cell culture was incubated with 5-ethynyl uridine (EU) for 10 (20) minutes. Total RNA was extracted using QIAzol lysis reagent. Nascent RNA labelled with EU was purified using Click-iT Nascent RNA Capture Kit. NEB NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770) was used for library preparation.