Disease oral swab 16s rRNA sequencing sample 3
The murine oral cavity was swabbed for 30 seconds, using sterile fine tip rayon swabs (VWR International) after the animal was culled, and the swabs were stored in 100 l reduced Johns transport medium at -70C. Whole genomic DNA was extracted from the above swabs using the DNeasy PowerSoil Pro Kit (Qiagen) according to manufacturers instructions. PCR reactions targeting the V1-V2 variable regions of the 16S rRNA gene were performed as previously described in Joseph et al. (2021)