GSM7986000: KO-YR714; Mus musculus; RNA-Seq
Total RNAs were extracted using the Rneasy mini kit (Qiagen) according to the manufacturer's protocol. RNA yields and quality were determined using a NanoDrop ND-1000 Spectrophotometer and an Agilent 2100 Bioanalyzer. Stranded RNA-Seq libraries were constructed from 100 ng of high-quality total RNA (RIN > 8) using the TruSeq Stranded mRNA Library Preparation Kit (Illumina)