GSM7785664: bladder cancer-MIBC-3; Homo sapiens; RNA-Seq
Total RNA was extracted from FFPE tissues followed by library preparation according to Illumina standard instruction (SMARTer Stranded Total RNA-Seq Kit v2). Agilent 4200 bioanalyzer was employed to evaluate the concentration and size distribution of cDNA library before sequencing with an Illumina NovaSeq6000. The protocol of high-throughput sequencing was fully according to the manufacturer's instructions (Illumina).