GSM8061347: RKO cells, shTRIM21, biol rep1; Homo sapiens; RNA-Seq
Total RNA was isolated from the colorectal cancer cells using Trizol reagent (Invitrogen; ThermoFisher) according to the manufacturer's instructions. Then, the ribosomal RNA was depleted from the Trizol-harvested total RNA with the GenSeq rRNA Removal Kit (GenSeq, Inc.) by adhering to its operation guideline. Next, RNA-seq libraries (with each treatment in triplicate) were constructed with the GenSeq Low Input RNA Library Prep Kit (GenSeq, Inc.) following the manual specifications.