GSM8012759: RNA-seq from skin CD4+ CCR6+ Th17 cells following S.epi treatment (rep2); Mus musculus; RNA-Seq
Tissues were harvested and cells were extracted (see manuscript for details) and sorted. T cells were sorted as CD4+ CD25- CCR6- for Th1 cells and CD4+ CD25- CCR6+ for Th17. Keratinocytes were sorted as  LD-Cd45-Cd31-Cd34-Cd49f+Sca1+. For more details for the protocol used to sort T cells refer to Linehan et al, 2018; For more details for the protocol used to sort keratinicytes refer to Lima-Junior et al., 2021 For T cells, cells were processed and analyzed for RNA-seq as described in Linehan et al., 2018. For keratiocytes: Keratinocytes from Lck-Cre Ramp1 fl/fl and WT mice were sorted as described in Lima-Junior et al., 2021. For T cells: mRNA libraries were constructed using SMARTer Ultra Low Input RNA kit (Clonetech) + Nextera XT DNA library prep kit (Illumina). For keratinocytes: RNA libraries were construted using the mouse Tecan SoLo library preparation kit.