RNAseq of Streblospio benedicti embryos
Embryo RNA extraction used the Arcturus PicoPure (Ref: 12204-01) including the DNAse step. We used a Qubit RNA kit to measure RNA yields for pilot data, but once we could estimate RNA yield per embryo we bypassed this step to maximize RNA yields. Libraries were constructed with the NEB UltraII Stranded RNA library prep kit (cat# E7760S) for Illumina. Libraries were sequenced on two lanes of 150bp on the Illumina NovaSeq.