NextSeq 500 sequencing; RNA-seq of Arabidopsis thaliana seedlings to investigate ZAT14 inducible misexpression - TRANSPLANTA collection
RNA-seq of Arabidopsis thaliana seedlings to investigate ZAT14 inducible misexpression - TRANSPLANTA collection
collection of Arabidopsis seedlings grown on ½ MS agar plates 8 hours after treatment with DMSO (1:1000, mock) or β-estradiol (50μM). Col-0 wild type plants or ZAT14 misexpressing plants with DMSO (mock) or β-estradiol (50μM) to induce ZAT14 expression total RNA extraction with a Spectrum Plant Total RNA Kit Library preparation was done using the TruSeq stranded RNA Sample Preparation Kit v2 (Illumina).