GSM7428951: NANS-KO iPSCs-derived cerebral organoids, Day35, 2; Homo sapiens; RNA-Seq
RNA was harvested using miRneasy mini kit (Qiagen). The quantity of RNA samples was analyzed by NanoDrop 2000c (Thermo Scientific). The mRNA was extracted using an RNA sample preparation kit (Illumina). A double-stranded cDNA synthesis kit (Invitrogen) was used to produce the cDNA and 200–300 bps sheared cDNA was inserted into the library.