GSM7593221: 2: iBMDM cells, control-2; Mus musculus; RNA-Seq
Total RNA was extracted with TRIzol Reagent (Thermo Fisher Scientific, 15596026) according the manufacturer's instructions as described. 20 to 30 μg of total RNA each group was used for the construction of sequencing libraries. RNA-seq libraries were prepared using the VAHTS mRNA-seq V3 library prep kit (Illumina) according to the manufacturer's instructions.