lake sediments - 18S
Total cDNA was submitted to the University of Minnesota Genomics Center (UMGC) where libraries dual indexed Nextera XT DNA were prepared following Gohl et al 2016. Amplicons were sequenced at UMGC using MiSeq Illumina 2 x 300 bp chemistry with the primers 515Ff and 806rB targeting the V4 region of bacterial and archaeal 16S SSU rRNA gene sequences (Caporaso et al., 2012; Apprill et al., 2015) and primers E572F and E1009 targeting the V9 region of eukaryotic 18S SSU rRNA gene sequences (Comeau et al., 2011).