GSM7096233: Rat cortical tissue, Sham group [T08]; Rattus norvegicus; RNA-Seq
Total RNA was extracted using a TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA), and quality control was performed using an Agilent 2100 instrument. The Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA) were used to remove rRNA from high-quality total RNA. A total amount of 1.5 μg RNA per sample was used as input material for rRNA removal using the Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA). Sequencing libraries were generated using NEBNextR UltraTM Directional RNA Library Prep Kit for IlluminaR (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.