GSM6600051: MDD_3A; Homo sapiens; RNA-Seq
The flash-frozen human hippocampus brain slides were taken from the -80˚C freezer and placed on a bench at room temperature for about 10 minutes. 1X phosphate buffer saline (PBS) supplemented with RNase inhibitor (0.05U/µL, Enymatics) was used to wash the tissue. Following this, they were fixed with 4% formaldehyde (Sigma) for 20 minutes at room temperature. The tissues were once again cleaned with the RNase inhibitor (0.05U/µL) in PBS, and then permeabilized with 1% Triton X-100 in PBS. Afterwards, the tissue slides were dipped in diH2O and then gently dried with nitrogen air. MRNA library was contructed following our DBiT-seq protocol (Liu et al., Cell, 2020) (https://doi.org/10.1016/j.cell.2020.10.026).