CRC organoid, Coculture wCAF, 5FU rep2
The experimental design involves establishing colorectal cancer organoids and co-culturing them with cancer-associated fibroblasts (CAFs) to mimic the tumor microenvironment. The co-culture system was treated with anticancer drugs 18-FU and oxaliplatin to study drug resistance mechanisms.  Sequencing was performed on a total of six samples: drug-treated, untreated, monoculture, and co-culture.