RNA-seq of Malus Domestica: fruit peel of upper mutant branch of RubyMac
Fruit skin was obtained using a handheld fruit peeler to separate the skin (as well as a few millimeters of cortex tissue) from the fruit. Tissue was ground in liquid nitrogen using a mortar and pestle and stored at -80 C until use. Total RNA was extracted from according to Gasic et al. (2004) and adapted for microcentrifuge tubes by using 1/10 the recommended volumes of all solvents and sample weights. Following the initial extraction, the extracted RNA was purified using the RNeasy mini-spin column protocol (Qiagen, Hilden, Germany). RNA samples were sequenced at The Max Planck Genome Center Cologne.