GSM7729203: α- ApoE3Ch/PSEN1WT, scRNA-seq; Homo sapiens; RNA-Seq
Six organoids from each individual cell line (eight lines in total) were pooled and processed using MACS papain neural tissue dissociation kit (Miltenyi Biotec #130-092-628) according to manufacturer's protocol and resuspended in 0.22μm filter sterilized dPBS 0.04%BSA solution. Cells were serially filtered through a 70μm (Miltenyi Biotec #130-110-916) then 40μm (Bel-Art #H136800040) strainer to remove clumped cells. Samples were kept on wet ice and assessed at the BioMicroCenter core facility (Massachusetts Institute of Technology) for viability, cell density, and quality. Samples were loaded (target 10,000 cells) on a Chromium Controller (10x Genomics). Library construction and sequencing were performed using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 with cDNA amplified 12 cycles using standard conditions. Libraries were sequenced on 2 lanes of a NovaSeq 6000 (Illumina) S4 flowcell at 150PE.