GSM7311693: Intact-2N-4_S5; Ambystoma mexicanum; RNA-Seq
Cells were dissociated in dissociation buffer (100 mg/ml liberase diluted in 0.7X PBS with 0.5 U/ml DNase I) for 30 min at room temperature with gentle mixing. Cells were then stained with 1 mg/ml Hoecsht, calcein AM, and propidium iodide for isolation of alive, dividing (4C) and non-dividing (2C) cells by fluorescence activated cell sorting. The 2C cells were used for the downstream library preparation. 10x Single Cell 3' v3.1 NextGEM library preparation protocol NovaSeq_S4_single_lane 2 x 100 bp