GSM7595076: Back, E15.5, rep1; Mus musculus; RNA-Seq
Total RNA was extracted using RNA extraction kit (Takara, Japan) and the concentration was determined using the NanoDrop ND-1000. 2 μg of total RNA per specimen was used to construct the RNA-seq library. The RNA-seq libraries were prepared using a Illumina TruSeq RNA Sample Preparation Kit and sequenced on the Illumina NovaSeq 6000 platform with PE150 at Frasergen Bioinformatics.