GSM7594291: MCF10A_M3c; Homo sapiens; RNA-Seq
Total RNA was isolated with TRIzol reagent (Ambion) according to manufacturer protocol. RNA quality was examined by the 4200 TapeStation (Agilent Technologies) and RNA concentration was determined using the Qubit Fluorometric Quantitation (Thermo Fisher). Stranded cDNA libraries were prepared using the TruSeq Stranded Total RNA prep, Ligation with Ribo-Zero Plus kit (Illumina) in accordance with the manufacturer's protocol, with ribosomal RNA depletion.