GSM7729876: SRSLY_BR_V; Brachylophosaurus canadensis; Hi-C
Tyrannosaurus rex (MOR1125) and Brachylophosaurus canadensis (MOR 2598) were prepared and analyzed in separate laboratories from the extant material, with separate buffers, reagents and instruments, by investigators wearing all protective lab garb (coats, hair covers, masks, gloves, etc.) and following aseptic procedures. Samples were prepared in the BioGard hood. Cortical bone fragments from each dinosaur were subjected to demineralization by incubation in 0.5 M EDTA (pH 8.0). Debris remaining from demineralization was filtered through a 40 µm cell strainer (BD Falcon 352340), and filtrate was centrifuged and washed with E-pure water to collect cells. Vessels were isolated from debris trapped within the cell strainer and washed. Both cells and vessels were incubated with the iron chelator pyridoxal isonicotinoyl hydrazone (PIH) in solution (5 mM PIH in 50 mM NaOH) (1,2,3) to remove iron, and then were prepared with "Protocol for preparing non-mammalian samples for Omni-C sequencing and do novo genome reconstruction— 2/22/2021, adopted by Berkley Gryder from Dovetail protocol used in the DNA zoo project" beginning at step 3, as above. KAPA HyperPlus Kit protocol (Roche 07962401001)