GSM7729859: S_6_B5_2; Homo sapiens; RNA-Seq
RNA was isolated and purified using the RNeasy MiniKit (Qiagen) and treated with DNase (Qiagen). RNA concentration (A260) and purity (A260 A280 ratio) were measured by spectrophotometry (NanoDrop™ 2000 Spectrophotometer, software version 1.6.198, ThermoFisher Scientific). RNA integrity number (RIN) was evaluated using an Agilent 2100 Bioanalyzer® (Agilent Technologies). All RNA samples showed very good quality as indicated by high RIN values between 9.0 and 10.0. TempO-SeqR software package according to manufacturer´s recommendation (BioClavis)