GSM6600464: MHB_1; Pseudomonas aeruginosa PAO1; Tn-Seq
Genomic DNA was extracted using the Dneasy Blood and Tissue kit (Qiagen). Concentration and purity was measured using a NanoDrop 2000 (ThermoFisher). Four replicate PCR reactions were used to amplify the transposon-genome junctions and were pooled together. Two-sided size selection was performed with Agencourt AMPure XP magnetic beads. Illumina Nextera Index Kit v2 SetA was used for indexing.