GSM7140689: LTi-like ILC3 from the mLN of Rorc-Cre x Bcl6-fl/fl mice, biol rep 2; Mus musculus; RNA-Seq
For small intestine lymphocyte isolation, fat and Peyer's patches were removed and the small intestine was opened longitudinally and washed vigorously in PBS. Tissue was incubated for two concurrent rounds in PBS containing 1 mM EDTA, 1 mM dithiothreitol and 5 % FBS for 25 min at 37 °C with constant mixing. Then, tissues were washed in PBS and digested in complete RPMI with in 1 mg/ml collagenase VIII (Sigma-Aldrich) and 20 μg/ml DNase (Sigma-Aldrich) for 25 min at 37 °C. For mLN, fat was removed and lymph node was digested in 0.33mg/mL Liberase TL (Roche) and 1mg/ml DNase (Sigma-Aldrich)  for 25min at 37ºC on agitation. All the digested tissues were filtered through a 70 μ