GSM7430850: Nucleoli -Rep1; Homo sapiens; OTHER
Nucleoli and Amyloid-bodies were purified and then RNA was extracted using TRIzol. Poly(A)-RNA was selected (Illumina Truseq mRNA preparation kit), followed by GI tailing (USB poly(A) tail length assay, Thermo Fisher), cDNA synthesis (SMARTscribe). Samples were barcoded and prepared using Pacbio sequencing (SMRTbell Template Prep Kit).