GSM7505865: AD3_D35D; Homo sapiens; OTHER
Fresh frozen sections of four fetal retina samples of 8, 10, 11 and 13PCW were used for the spatial transcriptome analyses performed with the Visium Spatial Gene Expression kit from 10XGenomics. First the tissue optimisation was performed defining 30 minutes as the most optimal permeabilization time window. The spatial transcriptomic procedure was performed according to manufacturer's instructions. Four tissue sections from each sample were carefully placed into the four capture areas, fixed, haematoxylin and eosin stained and images in order to preserve histological information. This makes it possible to overlay the cell tissue image and the gene expression data in a later step. After permeabilization, reverse transcription reagents were added on top of the tissues. The tissues were subsequently removed, leaving the cDNA coupled to the arrayed oligonucleotides on the slide. The cDNA-RNA hybrids were cleaved off the chip and the sequencing libraries were prepared. The sequencing depth varied between 25,000,000 and 150,000,000 million reads.