GSM7140979: macrophage_5ng_GM-CSF-1; Mus musculus; RNA-Seq
RNA from the sorted TAMs and CD8 + T cells was isolated using RNeasy Plus Mini Kit (Qiagen 74136) and further concentrated using RNeasy MinElute Cleanup Kit (Qiagen 74204). Library preparation and sequencing were performed at the Genomics Core Facility of West Virginia University and  Marshall University, respectively. When building these libraries we started with RNA that was too low to quantify as it was extracted from a small number of cells. THe libraries were built with NEB's Low Input mRNA prep and Illumina compatible UDI's (also from NEB) following manufacturers suggested protocol. Generate cDNA with 21 cycles. After cDNA synthesis/amplification, bring 10ng amplifies cDNA into the fragmentation/end prep step.   Use 8 cycles PCR for final amplification.