GSM7057118: subcutaneous,17,317; Homo sapiens; RNA-Seq
The adipose tissue was placed in PBS buffer, and then the cells were dissociated and placed in BD Rhapsody platform for single-cell sequencing The pooled cells were loaded on a BD Rhapsody™ Cartridge for capturing the single cells by pairing one cell with one barcoded beads. Then the cells were lysed to release mRNA, which were captured by barcoded oligo on bead, which has the cell label information and unique molecular identifiers information. Then the beads were retrieved from Cartridge into one tube and performed reverse transcription. The cDNA of captured mRNA was first synthesized on BD Rhapsody™ Cell Capture Beads as described in the instrument user guides. At the same time, the barcode information from BD Rhapsody Cell Capture Beads is also added to Sample Tags during reverse transcription.