RNA-seq of flow sorted SOX17-tomato+ naive iPSC OS1 cells following 24hrs PDA83 and 24hrs FA83X treatment
Low quality bases and adapters in sequenced reads were trimmed. Trimmed reads were mapped to the hg38 human reference genome using STAR, with 108 ENSMBL release .gtf file. FeatureCounts from SubRead v2.0.0 were used to to quantify expression to gene loci. Normalisation was carried out using DESeq2.