GSM7430910: TDP-43 KD, Rep 6; Homo sapiens; RNA-Seq
The cells were subsequently washed twice with ice cold PBS and further processed with TRIZOL for RNA analysis. Total RNA was isolated using Tri-Reagent. RNA quality was assessed using Nanodrop 2000 (Thermo Fisher Scientific, GmbH) and RNA integrity number (RIN) was evaluated by using the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA). RNA was reverse transcribed to cDNA using Transcriptor High Fidelity cDNA synthesis kit (Roche Diagnostics Deutschland GmbH, Mannheim, Germany)  RNA-Seq libraries were obtained using TruSeq RNA Sample Preparation v2 kit (Illumina, Inc., San Diego, USA). To analyze the library quality, Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, USA) was used and the concentration was measured by a QubitR dsDNA HS Assay kit (Thermo Fisher Scientific Inc., Waltham, USA).