GSM8105714: adult brain, wildtype CT control-3; Drosophila melanogaster; ATAC-seq
~50 fly brains were dissected and dissociated with collagenase. Single cell suspension was sorted on a BD Aria III sorter to isolate clock neurons (GFP+ DAPI-) and non-clock cells (GFP- DAPI-). Sorted clock neurons were immediately used for ATAC assay. Transposed DNA was amplified using limited-cycle PCR. Ampliication cycle numbers were determined by qPCR to make sure exponential phase of amplification. Amplified libraries were purified by non-selective SPRI bead purification and size distribution was assessed by gel electrophoresis(Agilent Tapestation, high-sensitivity DNA 5000 assay). If excess adapter dimers were observed, an extra size-selective SPRI purification was performed.