GSM7429560: K14KCTD15KO [49656]; Mus musculus; RNA-Seq
Total RNA was isolated with Trizol reagent and then with Zymo Direct-zol RNA Mini Prep Plus and DNase treatment For RNA-seq library construction, rRNA depletion sequencing library was prepared by using QIAGEN FastSelect rRNA HMR Kit (Qiagen, Hilden, Germany). RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturer's recommendations (NEB, Ipswich, MA, USA). Sequencing libraries were validated using the Agilent Tapestation 4200 (Agilent Technologies, Palo Alto, CA, USA), quantified using Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA), and loaded to Illumina NovaSeq 6000 system for 150bp paired-end sequencing.