Bile-induced biofilm formation in Bacteroides thetaiotaomicron requires magnesium efflux by an RND pump
Bile-induced biofilm formation in Bacteroides thetaiotaomicron requires magnesium efflux by an RND pump
Overnight cultures were mixed with RNAprotect (Qiagen) in the anaerobic chamber to prevent RNA degradation and bacteria were lysed using QIAGEN Proteinase K and TE buffer containing lysozyme.  Total RNA was extracted using the Direct Zol kit (Zymo Cat. R2050) according to the manufacturerâ€™s instructions and treated with DNase I from the same kit. RNA concentration, quality, and integrity from 4 independent replicates was checked using RNA6000 Nano chips and the Agilent 2100 Bioanalyzer system ribozero plus was used for ribosomal RNA depletion