Total_I1
Libraries were constructed according to the Mim-tRNAseq  protocol derived from Behrens et al. 2021(doi.org/10.1016/j.molcel.2021.01.028). For each sample, a different RNA adaptor was ligated at the 3' end of tRNAs. The six adaptor-ligated-tRNA samples were pooled and then retro-transcribed with TGIRT III. The cDNA was then circularized using CircLigase and amplified using Illumina adapters. The library was size-purified on a 6% polyacrylamide gel, quality was verified with a Bioanalyzer  and quantified with a Qubit device.