GSM8060348: rna_AID_CSR1_1mM_rep2; Caenorhabditis elegans; RNA-Seq
Worms were collected in TriReagent (ThermoFisher) and were subjected to three freeze-thaw cycles. Phase separation was achieved via 1-bromo-3-chloropropane (BCP) and the aqueous phase was precipitated in isopropanol at -80°C for 2 hrs. To pellet RNA, samples were centrifuged at 21,000 x g for 30 min at 4°C. The pellet was washed three times in 75% ethanol then resuspended in water. RNA-seq libraries were generated using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer's instructions.