GSM7428025: d14 HCO_3; Homo sapiens; RNA-Seq
Bulk RNA was extracted using an RNeasy Plus Micro Kit (Qiagen) according to manufacturer guidelines. 200 ng of RNA prepared into libraries following Illumina Stranded mRNA Prep protocol with all recommended reagents. Final library amplification step using 5 uL index anchors and 13 PCR cycles.