GSM7316632: 536, 1h, R1; Homo sapiens; RNA-Seq
Total RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB) with in-tube DNase treatment after elution of the RNA. The Monarch RNA Cleanup Kit (NEB) was used for subsequent purification of the RNA. Quality and quantity of the samples were evaluated on the Agilent Bioanalyzer using the Agilent RNA 6000 Nano Kit. The NEBNext Ultra II Directional RNA Library Prep Kit (NEB) was used for library preparation following the manufacturer's instructions.