Long- read sequencing of serum HBV RNA in patient 3
PCR product was purified by 0.6x AMPure PB Beads and constructed the library by using the SMRTbell Express Template Prep Kit 2.0 (PacBio) with damage repaired, end repaired, A-tailing, and ligated the sequencing adapters according to manufacturer's instructions. The SMRTbell library was then purified by AMPure PB beads.