GSM7316498: HAP1 sgRNA3 + HDR temp passage 0 Rep 3; Homo sapiens; OTHER
Total genomic DNA was isolated using the Macherey-Nagel NucleoSpin Tissue Mini DNA extraction kit. Genomic DNA was amplified using PCR primers outside the KARS1 Exon 2 locus (~3 kb product) followed by direct amplification of the exon 2 region of interest using region-specific primers with Illumina-compatible adapter overhangs. Resultant PCR products were amplified with Illumina i5/i7 universal primers and unique dual index primers. Fixed-amplicon sequencing with KAPA HiFi polymerase and Illumina-compatible adapter overhangs