GSM7506032: R3 DC Day9 CD11cCre Pikfyvef/f; Mus musculus; RNA-Seq
Total RNA was extracted using the Qiagen miRNeasy mini kit with the inclusion of the genomic DNA digestion step with the RNase-free DNase Kit. Depletion of rRNA using RiboErase selection kit from Kapa Biosystems was performed. RNA libraries were then generated using the KAPA RNA HyperPrep Kit from Roche according to manufacturer's protocols.