WGS of Kyphosus sydneyanus hindgut G233 (Section V)
The PowerSoil Pro kit (QIAGEN, Germantown, MD, United States) was used to extract DNA from 250uL of each sample (18 samples). DNA was visually inspected using 0.8% agarose gel electrophoresis. DNA libraries were prepared at Otago Genomics Facility (University of Otago, Dunedin, New Zealand) with the Takara Thruplex DNA-Seq 96D kit (Takara Bio, Kusatsu, Shiga, Japan). Paired 125 bp reads were generated using the Illumina HiSeq V4 platform (Illumina, San Diego, CA, United States) yielding 442.4 Gbp of sequence.