GSM6793160: imb_koenig_2018_18, HeLa, WT, 2018, rep2; Homo sapiens; RNA-Seq
Rneasy PLUS Mini Kit (Qiagen) "imb_koenig_2018_18: NGS library prep was performed with Illumina's TruSeq stranded total RNA LT Ribo Zero Gold Sample Prep Kit following Illumina's standard protocol (Part # 15031048 Rev. E). Libraries were prepared with a starting amount of 1000 ng and amplified in 10 PCR cycles. Libraries were profiled in a DNA 1000 Chip on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies). All 4 samples were pooled in equimolar ratio and sequenced on an Illumina NextSeq 500 sequencing machine as 84 nt single-end reads. imb_koenig_2020_12: NGS library prep was performed with Illumina's TruSeq stranded Total RNA LT Sample Prep Kit following Illumina's standard protocol (Part # 15031048 Rev. E). Libraries were prepared with a starting amount of 200 ng and amplified in 12 PCR cycles. In addition, 1 µl of ERCC spike-ins (1:250 dilution) was added to every sample. Libraries were profiled in a DNA 1000 kit on a 2100 Bioanalyzer (Agilent technologies) and quantified using the Qubit dsDNA HS Assay Kit, in a Qubit 2.0 Fluorometer (Life technologies). All 12 samples were pooled in equimolar ratio and sequenced on an Illumina NextSeq 500 sequencing machine as 159 nt single-end reads.