GSM8146325: Btg4wt-2; Mus musculus; RNA-Seq
Transcriptome profiling.The zona pellucidae of oocytes were removed with 0.5% pronase E (Sigma). 15 oocytes per sample (3 samples for each group) were lysed and subjected to first strand synthesis. Transcriptome profiling. The single-strand RNA molecules were reversed with oligo (dT) primer. Amplified cDNA was fragmented into ~300 bp fragments and then constructed by TruePrep DNA Library Prep Kit (Vazyme).