16S rRNA gene sequencing of fecal sample from orthotropic pancreatic ductal adenocarcinoma (PDAC) C57BL/6J bearing mice 3 weeks after tumor implantation
16S libraries were generated following the Earth Microbiome Project ( https://earthmicrobiome.org/protocols-and-standards/16s/). Pooled libraries were sequenced (Illumina MiSeq) at loading concentration of 7pM with 15% PhiX, paired-end 250 (MiSeq Reagent Kit v2, 500-cycles).