GSM6642060: 1 month cerebellum from KO mouse 3 [S_CB_KO3]; Mus musculus; RNA-Seq
Mouse brain tissue were dissected on ice, fast froze in liquid nitrogen, and stored in -80˚C until use. On the day of RNA extraction, 50mg to 100mg tissue from each sample was lysed in 1ml TRIzol (15596026, Invitrogen). Addition of 0.2ml chloroform (288306, Sigma-Aldreich) followed by centrifugation was performed to separate the solution into an aqueous phase and an organic phase. Isopropyl alcohol and 75% ethanol (111000200CSPP, Pharmco) was added in sequence to precipitate and wash the RNA pellets. A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA  Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.