GSM7430937: OPC_Dor; Rattus norvegicus; OTHER
Cut&Tag libraries preparing and sequencing. To prepare sequencing libraries of Cut&Tag, fresh specimens from rat were processed following the protocol of Hieff NGS® G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina® (Yeasen Biotech Co., Ltd.). Library concentration was measured using Qubit® 2.0 Fluorometer (Life Technologies, CA, USA), and library fragment analysis was assessed using the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Qualified libraries were sequenced on Novaseq 6000 sequencer (Illumina) with 150 bp paired-end reads.