GSM6737933: xSCAR_442_H2AZ_T0_r2_A+B; Mus musculus; OTHER
For SCAR samples: After the appropriate treatment, cells were collected by scraping and nuclei were isolated. Chromatin was digested with MNase. Sequentially, mononucleosomal fragments were immunoprecipitated, purified fragments were biotinylated, libraries were prepared, EdU-biotin fragments were pulled-down and sequenced. For xSCAR samples (H2A.Z, H4K20me0 when indicated, JARID2): cells were crosslinked for 10min with 1% formaldehyde, quenched, nuclei extracted and sonicated using Covaris. Sequentially, fragments were immunoprecipitated, purified, fragments were converted to libraries, biotinylated and EdU-biotin fragments were pulled-down and sequenced. OTHER