16S of C. vandenburghi: microbiome carapace with shell disease 1
Samples collected by scraping the tortoise carapace. DNA extracted with the DneasyPowerSoil Pro Kit (Qiagen) with modifications. 16S region amplified employing the 16S Barcoding kit (ONT) and purified with DNA Purification SPRI Magnetic Beads (Applied Biological Materials Inc). Sequencing performed with the MinION device using Spot-ON Flow Cell and Flonge Flow Cells.