DNAJB1 trigger sequencing (SI Fig 2l) in the condition 450 ng DNAJB1.s1 + 50 ng ADAR1-p150 + VCL siRNA + heat shock
mRNA was extracted and reverse transcribed. The resulting cDNA was PCR-amplified using locus-specific primers for the sensor or trigger, gel purified, and shipped to Genewiz for Amplicon-EZ sequencing.