GSM7986436: ATAC_Col-bio1; Arabidopsis; ATAC-seq
Approximately 40,000 protoplasts were used for the isolation of nuclei. The purified nuclei were then incubated with Tn5 transposome and tagmentation buffer at 37 °C for 30 min Genomic DNA breaked by Tn-5 transposable enzyme was used for ATAC-seq library construction. Blunting end, adapter ligation and amplification were carried out using TruePrep DNA Library Prep Kit V2  according to the manufacturer's protocol. Illumina Novaseq was used for high-throughput sequencing of the ATAC-seq library.