GSM7729567: Ribo eIF2A∆+SM replicate 1; Saccharomyces cerevisiae; OTHER
Ribosome profiling (Rnase I footprinting, size selection, RNA extraction, preadenylated linker ligation, reverse transcription, cDNA circularization, PCR amplification) was done as described in "eIF1A residues implicated in cancer stabilize translation preinitiation complexes and favor suboptimal initiation sites in yeast" Martin Marcos et al. (2017) eLife 6:e31250 Ribosome-protected RNA 25-34 nt