GSM8321955: Cd11c-active; Mus musculus; RNA-Seq
Total RNA was prepared with the RNeasy Plus Kit (Qiagen 74034) and RNA Clean and Concentrator kit (Zymo R1013) and hybridized with NEBNext rRNA Depletion Kit v2 (E7400) to substantially diminish rRNA from the samples. Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit (Illumina E7760L). Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583). The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized to 5 nM in preparation for Illumina sequence analysis.