GSM6642046: 236R_KDM6A [scATAC-seq]; Mus musculus; ATAC-seq
dissociated cells were resuspended in DPBS with 0.04% UltraPure BSA and cell counts were measured with a Vi-CELL XR Cell Viability Analyzer (Beckman Coulter). Cells were then diluted in 0.04% BSA/DPBS at a cell concentration of 1000 cells/μL. About 16,000 cells were loaded onto a 10× Genomics ChromiumTM instrument (10× Genomics) according to the manufacturer's instructions nuclei were transposed according to 10XGenomics specifications. Approximately 10,000 cells were targeted for each tissueanalyzed and pooled and processed according to the 10x Genomics scATAC-seq sample preparation protocol (Chromium Single Cell ATAC Library & Gel Bead Kit, 10x Genomics) to obtain a single sequencing library