GSM7430647: DMSO_NoAgg, biol rep 2; Mus musculus; RNA-Seq
RNA was harvested using easy-BLUE (iNtRON Biotechnology), under protection from direct light. After conjugating 4SU to iodoacetamide  in a 50 mM pH 8.0 phosphate buffer in DMSO/Water (1:1), the reaction was quenched with DTT and RNA was reprecipitated using ethanol and NaOAc. Libraries were prepared using Quantseq 3' mRNA-Seq Library Prep Kit (Lexogen).