GSM7315584: liver metastatic tissue_1; Mus musculus; OTHER
The genomic DNA was extracted with a DNeasy Blood & Tissue Kit (Qiagene). The extracted genomic DNA was subjected to the initial round of 24 cycles of PCR to amplify sgRNA sequences. Amplicons from the first PCR were used in a second PCR of 8 cycles to add index and adapter sequences for Illumina sequencing. The final sequencing libraries were quantified using the Qubit 3 Fluorometer (Thermo Fisher) and assessed for size using the 2200 TapeStation System (Agilent).