GSM7677075: Ant No. 54 , Size: 5376.84 µM, EM-seq; Camponotus floridanus; OTHER
DNA and RNA (including miRNA) were extracted with the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN) according to the manufacturer's instructions. gDNA was quantified using the Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies). Libraries were generated from 200ng of gDNA using the NEBNext® Enzymatic Methyl-seq Kit (New England BioLabs).Adapters were included in the library kit. The libraries were normalized and pooled and then denatured in 0.05N NaOH and neutralized using HT1 buffer. The pool was loaded at 225pM on an Illumina NovaSeq S4 lane using Xp protocol as per the manufacturer's recommendations. The run was performed for 2x150 cycles (paired-end mode). A phiX library was used as a control and mixed with libraries at 5% level.  Libraries were quantified using the KAPA Library Quanitification Kits - Complete kit (Universal) (Kapa Biosystems). Average size fragment was determined using a LabChip GXII (PerkinElmer) instrument.