GSM7312228: D14_TRIM28_gRNA02 [scATAC-seq]; Homo sapiens; ATAC-seq
Cells on Day 14 of differentiation were harvested, washed with FACS buffer (PBS + 2% FBS), and stained with DAPI for 10 minutes at room temperature. Viable DAPI- cells were isolated by FACS using a Sony MA900. scATAC-Seq targeting 5,000 cells per gRNA was performed using the Chromium NextGEM Single Cell ATAC Reagent Kit v1.1 (10X Genomics) per the manufacturer's instructions. The concentration of each final library was quantified using a High Sensitivity DNA Bioanalyzer Assay (Agilent). scATAC-Seq libraries were equimolar pooled and sequenced on a NextSeq 550 in a 34-8-16-34 read configuration.