Metabarcoding of microbial 16S fromPCR MMIx negative.
Microbial eDNA was amplified using the primers 515f (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGCCAGCMGCCGCGGTAA-3) and 806r (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGGACTACHVGGGTWTCTAAT-3) from Caporaso et al. (2011), with Nextera compatible adapter tails on the five prime ends.