GSM7787693: abf1/2/3/4 mutant root, control, ATAC-seq replicate2; Arabidopsis thaliana; ATAC-seq
Tagmentation (Tn5-based fragmentation) was performed on FACS isolated nuclei for 30 minutes at 37 C. Tagged DNA fragments were purified using the Qiagen min-Elute PCR purification kit. ATAC-seq libraries were amplified with Nextera indexed primers for a limited number of cycles (determined by side-qPCR reaction). Amplified libraries were size-selected and purified using SPRI beads.