Targeted nanopore sequencing of PRMT1 circRNA-derived amplicons (ZR-75-1 cell line)
Total RNA from ZR-75-1 cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each PRMT1 exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.