Fungal microbiota of bulk soil
DNA extraction was performed using 30 mg of soil slurry and the NucleoSpin Soil Kit (Macherey-Nagel). The amplification reaction was carried out using the KAPA HiFi HotStart ReadyMixPCR Kit and targeting the fungal ITS2 region. Samples were sequenced on a Illumina MiSeq Standard Flow Cell.