GSM7058880: KO_4; Homo sapiens; RNA-Seq
Total RNA was extracted with an RNeasy Kit (Qiagen, Hilden, Germany), and RNA was quantified using a NanoDrop-2000c spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). The mRNA libraries were prepared in a strand-specific manner using NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat No. E7490) and NEBNext UltraTMII Directional RNA Library Prep Kit (Cat No. E7760).