GSM7429868: female Poecilia latipinna 9, whole brain, Ff treatment; Poecilia latipinna; RNA-Seq
Immediately following the 30-minute behavioral trial, focal females were immediately decapitated, whole brains were removed and individually immersed in RNAlater solution (Applied Biosystems, Carlsbad, CA, USA) at 4˚ Celsius with gentle shaking for 24 hours, then stored at -80 C (without RNAlater) for subsequent RNA profiling. 1-4 μg of total RNA were used as input for RNA library preparation and sequencing. RNA libraries for RNA-seq were prepared using Illumina's TruSeq mRNA Sample Prep Kit.