Single nucleus mRNA library generated from an osteoarthritis synovial tissue sample as part of a 10x single cell multimodal ATAC and Gene Expression experiment.
The osteoarthritis synovial tissue sample was processed separately in the cell capture step. Nuclei were isolated using an adaptation of the manufacturer's protocol (10X Genomics). Approximately ten thousand transposed nuclei were loaded on Chromium Next GEM Chip J followed by GEM generation. 10x Barcoded DNA from the transposed DNA (for ATAC) and 10x Barcoded, full-length cDNA from poly-adenylated mRNA (for Gene Expression) were produced during GEM incubation. The ATAC libraries and Gene Expression libraries were then generated separately. Both library types were sequenced to an average of 30,000 reads per cell on different flowcells with recommended sequencing cycles according to the manufacturer's protocol (Chromium Next GEM Single Cell Multiome ATAC + Gene Expression, 10X Genomics).