GSM7986224: YAP1, replicate 2; Mus musculus; RNA-Seq
BM cells from WT, YAP1 heterozygote mice were flow-sorted. Cells were loaded onto the Chromium single cell controller (10x Genomics) to generate single-cell gel beads in the emulsion according to the manufacturer's protocol. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual gel-bead-in-emulsions (GEMs). Single-cell RNA-seq libraries were constructed using Single Cell 3' Library and Gel Bead Kit V3.1.