GSM7428204: Uterine_neuroendocrine [OE180716630-02]; Homo sapiens; RNA-Seq
Single-cell library preparation was carried out using Chromium Single cell 3' Reagent v2 Kits (10 × Genomics, USA) according to the manufacturer's protocol. Cells were loaded on the Chromium Single Cell Controller Instrument to generate single cell gel beads in emulsions (GEMs). Next, reverse transcription was performed, cDNA was cleaned up with DynaBeads Myone Silane Beads (Thermo Fisher Scientific, USA), and was then amplified by PCR with appropriate cycles. Subsequently, the amplified cDNA was fragmented, end-repaired, A-tailed, index adaptor-ligated and subjected to library amplification.