GSM7593190: MII_rep_4; Sus scrofa; RNA-Seq
The SMART-Seq® HT kit (Takara Bio Inc., Shiga, Japan) was used for cDNA generation according to the manufacturer's instructions using 13 PCR cycles across a total of 15 samples. cDNAs were then converted to sequencing libraries using the Illumina DNA Prep kit (Illumina, San Diego, USA) according to the manufacturer's instructions with 8 PCR cycles for the final amplification. An equimolar pool of libraries was sequenced by single-end RNA sequencing (1 x 75bp) on an Illumina Nextseq 500 at the South Australian Genomics Centre (SAGC; Adelaide, SA).