16S rRNA of artificial seawater media after 1 week replicate 1
Microbial community composition was determined by 16S rRNA gene sequencing using primers targeting the V4-V5 region of the 16S rRNA gene (515F-Y 5-GTGYCAGCMGCCGCGGTAA-3, 909R 5-CCCCGYCAATTCMTTTRAGT-3) (Parada et al., 2016; Tuan et al., 2014; Walters et al., 2016; Wang et al., 2018). Illumina MiSeq 2 x 300 nt paired-end sequencing was performed with V3 chemistry (StarSEQ GmbH, Germany), including negative and positive controls.