GSM6600592: S2_unstimulated; Homo sapiens; RNA-Seq
Total RNA was extracted using Zymo Research Direct-zol RNA extraction kit RNA-seq libraries were prepared according to the SCRB-seq/3'DGE protocol, using oligo-dT capture followed by template switching and barcoded cDNA generation. Final libraries underwent hybrid capture enrichment based targeting 17 T-cell specific genes (IL2,IFNG,TNF,TGFB1,PRF1,GZMB,GATA3,TBX21,FOXP3,RORC,RUNX1,RUNX3,BCL6,IL10,IL12A,IL13,IL17A)