GSM7593162: SPT16_AID_V5_Clone2_IAA_TTseq_washout_Rep1; Mus musculus; OTHER
Medium was aspirated and cells were washed with DPBS. DPBS was aspirated and replaced with fresh medium containing 500 mM 4-thiouridine (4sU). Cells were incubated at 37 degrees Celsius with 5% CO2 for five minutes, then washed with DPBS and trypsinized. Cells were pelleted, washed with DPBS, and RNA was extracted with TRIzol per manufacturer's instructions. TT-seq; built with NEBNext Directional kit