GSM7430572: Young rosette 3; Arabidopsis thaliana; RNA-Seq
RNA was extracted from 100 mg of each plant material using innuPREP Plant RNA kit (Analytik Jena, Germany) according to manufacturer's protocol. Lysis Solution RL was used for young and mature rosettes, flowers and roots, while for stems and green siliques, lysis solution PL. DNase treatment was done with TURBO DNA-free Kit (Thermo Fisher Scientific, USA) and concentration was measured using DeNovix DS-11 series spectrophotometer (DeNovix Inc., United States). RNA (500 ng) from each plant material was provided to the Finnish Functional Genomics Center (FFGC, University of Turku and Åbo Akademi and Biocenter Finland) for PolyA+ transcriptome sequencing (RUO). The RNA integrity was checked with Agilent Bioanalyzer 2100 and the library preparation was done with Illumina Stranded mRNA Preparation kit (Illumina, USA). The paired-end libraries were sequenced using the Illumina NovaSeq 6000 S1 v1.5 (Illumina, USA).