GSM6738440: Test C-3; Mus musculus; miRNA-Seq
Exosomes were isolated from serum of uninfected mice (n = 20; namely un-Exos) and PbA-infected mice (n = 20; namely inf-Exos) at ~40% parasitaemia using the Total Exosome Isolation Kit (from serum; No. 4478360#, Invitrogen) following the manufacturer's protocol. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.