Targeted nanopore sequencing of PRMT1 circRNA-derived amplicons (Hs 578T cell line)
Total RNA from Hs 578T cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each PRMT1 exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.