GSM8059861: SW480-RSC (ROBO1neg), replicate 2; Homo sapiens; RNA-Seq
Cells were collected in TRIzol Reagent (Invitrogen) and stored at -80˚C until all replicates were collected. RNA was isolated with the Direct-zol RNA Miniprep Kit (Zymo Research) 1µg of RNA was used for library generation using Illumina TruSeq Stranded mRNA Library Prep kit following manufacturer's protocols.