16S bacterial community metabarcoding of pitcher plant fluid
To characterize microbial community composition, DNA was extracted and amplified for 16S rRNA amplicon sequencing using the DNAdvance Genomic DNA Isolation Kit (Beckman Coulter A48705). DNA was quantified using AccuClear Ultra HS dsDNA kit (Biotium #31028). Library preparation and sequencing was conducted by the Environmental Sample Preparation and Sequencing Facility at Argonne National Laboratory on a 151 bp x 12 bp x 151 bp Illumina MiSeq run targeting the V4 region of 16S rRNA using the 515F and 806R primers