Fungal microbiota of bovine feces
DNA extraction was performed using 70 mg of fecal matter and the NucleoSpin Soil Kit (Macherey-Nagel). The amplification reaction was carried out using the KAPA HiFi HotStart ReadyMixPCR Kit and targeting the fungal ITS2 region. Samples were sequenced on a Illumina MiSeq Standard Flow Cell.