GSM7100227: WT_2_YEPD; Saccharomyces cerevisiae; RNA-Seq
mRNA was extracted from exponentially growing cells using a RNeasy kit (Qiagen) and treated with DNaseI. Sequencing libraries of cDNA were made using the TruSeq stranded mRNA kit (Illumina) according to the manufacturer's protocol. The libraries were analyzed for size and quantity of cDNAs on a 2100 Bioanalyzer (Agilent), diluted and pooled in multiplex sequencing pools. The libraries were sequenced as 65 base single reads on a HiSeq2500 (Illumina).