GSM8061485: RV1B d4, rep 1; Mus musculus; RNA-Seq
Total RNA was prepared using Trizol extraction.  RNA was double purified using RNEasy spin columns and enriched for mRNA using a Poly(A) mRNA Magnetic Isolation Module. cDNA libraries for next generation sequencing were prepared with a xGen Broad-range RNA Library Prep with xGen Normalase UDI Primers. Samples were subjected to 151 bp paired-end sequencing using a NovaSeq S4 or NaveSeq X Plus flow cell according to the Illumina NovaSeq manufacturer's protocol.