GSM8322394: ThymoSphere (TS) week 9 CD3+ TCRγδ- cDNA; Homo sapiens; OTHER
At week 9, multiple TS-containing inserts were harvested and pooled. Subsequently, the CD3+ TCRγδ- fraction was sorted. Following FACS, the cells were resuspended at an estimated final concentration of 3000 cells/µl and loaded onto a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM) at the VIB Single Cell Core. The scRNA/TCR libraries were prepared using the GemCode Single Cell 5' Gel Bead and Library kit, NextGEM v2 version (10x Genomics) according to the manufacturer's instructions.