GSM7142292: scAT, NORMO_18; Homo sapiens; RNA-Seq
Total RNA was extracted from SAT using Trizol reagent (Invitrogen, Carlsbad, CA), according to the manufacturer's instructions. RNA quantity and integrity were estimated using the Qubit instrument (Invitrogen, Carlsbad, CA) and the Agilent 4200 TapeStation System (Agilent, Santa Clara, CA). RNA libraries were performed using the CORALL total RNA-Seq library Prep Kit (Lexogen, Vienna, Austria) and the RiboCop rRNA De-pletion Kit (Lexogen, Vienna, Austria) was used to remove rRNA. Libraries qualities were assessed with High Sensitivity D1000 ScreenTape Assay using the 4200 TapeStation System (Agilent, Santa Clara, CA, USA) and quantified with Qubit dsDNA HS Assay Kit (Invitrogen, Carlsbad, CA).