Illumina HiSeq 4000 sequencing; RNA-seq of MOLM-13 acute myeloid leukemia cell line treated with FIDAS-5
RNA-seq of MOLM-13 acute myeloid leukemia cell line treated with FIDAS-5
MOLM-13 cells were detached from by addition of trypsin. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. MOLM-13 cells were cultured in RPMI1640 supplemented with 20% fetal bovine serum and 1% penicillin/streptomycin. MOLM-13 cells were treated with 3 ÂµM FIDAS-5 for 48 hours Total RNA was extracted using Trizol (Invitrogen) and purified using Direct-zol RNA Purification Kits (Zymo Research). Library construction was performed using Illumina Truseq Stranded Total RNA library preparation with Ribozero rRNA depletion