GSM7099586: HCT15_Lowg_2; Homo sapiens; RNA-Seq
Total RNA was extracted from CRC cells using TRIzol Reagent (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), and mRNA was extracted. mRNA was extracted and sheared into 200-base fragments. The fragmented mRNA was reverse-transcribed into complementary DNA (cDNA), which was amplified by PCR to generate the cDNA library. The cDNA library was sequenced using the Illumina NovaSeq 6000 platform.