Replicate 2 of RAD51 CUT&Tag in HeLa, add PyBI
CUT&Tag was constructed as described (Zheng et al., 2024). The bead-bound cells were resuspended in 100 uL dig-wash buffer (0.05% digitonin, 0.1% BSA, 20 mM HEPES-KOH at pH 7.5, 150 mM KCl, 0.5 mM spermidine, 2 mM EDTA, 1 uL protease inhibitors) containing 100 nM G4P or 18 ug/mL recombinant anti-RAD51 antibody (ab133534, Abcam) or 10 ug/mL anti-53BP1 antibody (4937S, CST).