GSM7058933: 123941; Mus musculus; RNA-Seq
mRNA was isolated from dorsal and ventral hippocampi using the Qiagen RNAeasy kit in five batches, with groups counterbalanced across batches. RNA was assessed for quality using the TapeStation (Agilent, Santa Clara, CA). Samples with RINs (RNA Integrity Numbers) of 8 or greater were prepared using theKAPA mRNA Hyper prep (KAPA/Roche #KK8581) according to the protocol using 400ng input and 11 cycles PCR. Final libraries were checked for quality and quantity by TapeStation (Agilent) and qPCR using Kapa's library quantification kit for Illumina Sequencing platforms (catalog # KK4835) (Kapa Biosystems,Wilmington MA). The samples were pooled and sequenced on 50% of an Illumina NovaSeq S4 Paired-end 50bp, according to the manufacturer's recommended protocols.