Targeted nanopore sequencing of BAX circRNA-derived amplicons (MCF-7 cell line)
Total RNA from MCF-7 cell line was reversely transcribed using random hexamers. Next, multiple nested PCR assays using divergent primers, specific for each BAX exon, were performed. After library construction, 3rd generation (nanopore-based) sequencing was conducted using the MinION Mk1C platform and the Flongle adapter.