GSM7728755: CUT&Run KG1, DMSO, 6h, No primary antibody, Rep2; Homo sapiens; ChIP-Seq
Cells were pelleted, washed with DPBS, and nuclei were extracted with a hypotonic buffer. Unfixed nuclei were bound to ConcanavalinA beads, washed, followed by successive addition of primary antibodies followed by proteinA/proteinG-MNase. Cleavage of Mnase was initiated by Ca2+ addition and stopped by chelation. Fragments were released and deep sequencing libraries were prepared. CUT&RUN