GSM7140119: sample20_D0_ChAdOx1_-ve; Homo sapiens; RNA-Seq
Peripheral blood (up to 2.5ml) was collected into a PAXgeneTM RNA tube at vaccination (D0) and at SARS-CoV-2 diagnosis (CT). Total RNA was extracted using the PAXgene blood RNA kit (PreAnalytiX, Switzerland) on the QIAsymphony SP (Qiagen) with the PAX_RNA V5 protocol. Eluted RNA was quantified using the Agilent RNA ScreenTape assay on the 4200 TapeStation System (Agilent). PCR-cDNA libraries were prepared using the SQK-PCS110 PCR-cDNA-sequencing kit following the standard Oxford Nanopore Technologies (ONT) protocol. Samples were barcoded (#1-8) and sequenced on PromethION. 2 samples were loaded per flow cell (15 ng each), with the paired samples run together and the unpaired samples randomly split into pairs