genomic Lentzea sp. strain BCCO 10_0856
Strains were isolated on McBeth Scales (MBS) agar (for details see Chrokov et al., 2010) from bare soil developing on Miocene lacustrine clay deposited after brown coal mining. Cultures were maintained on yeast-extract-malt-extract agar (International Streptomyces project [ISP] 2 media, Shirling and Gottlieb, 1966) and as cell suspensions in 15% (v/v) glycerol stocks at -80C for long-term. Strains were deposited in the Culture Collection of Actinomycetes of the Biology Centre Collection of Organisms (www.actinomycetes.bcco.cz, BCCO). All strains were isolated from soil at the top of a reclaimed mine heaps in early stages of succession at the Sokolov brown coal mine district area in the Czech Republic. Weak antibiotic activity against gram-positive bacteria, Bacillus subtilis and Staphylococcus aureus were determined using disc diffusion method.Biomass for genomic analyses was collected from 72 h old cultures grown aerobically (150 rpm) on Luria-Bretani medium broth