GSM7987247: PBS-treated and α4β7-treated cells, load 2, GEX; Mus musculus; OTHER
Peyer's patches (PP) were individually collected and weighed. Each PP was incubated in collagenase D (Sigma-Aldrich, 400IU/ml) for 30 min and mechanically homogenized to obtain a single-cell suspension Libraries were prepared according to manufacturer's instructions using the Single Cell 3' v3 CellPlex Kit (10x Genomics). CITESeq with HTO only (no ADT)