16S Seq of SeRB in FGD
FGD wastewater was used as the inoculum and enriched on selenite-amended media anaerobically for 7 days at 40C. DNA was extracted utilizing the Qiagen Powersoil Pro Kit. Extracted DNA and kit blanks were amplified using universal primers targeting the V4 region of the 16s RNA gene. Purified 16S rRNA libraries were pooled and diluted to a concentration of 2nM. The pool was then denatured with fresh 0.2M NaOH. Libraries were further diluted according to the manufacturer's instructions and sequenced on an Illumina MiSeq with a 300 cycle V2 nano kit.