GSM7312490: Caco-2/15-Z1flag Postconfluent cells IP Flag Exp2; Homo sapiens; ChIP-Seq
For ChIP-seq experiments, Caco-2/15 cells expressing Flag-H2AZ.1 and Flag-H2AZ.2, 100 mg of chromatin supplemented with 10 ng of Spike-in chromatin (Active Motif) were used per ChIP experiment. For each reaction, 4 µg of Flag M2 antibody (Sigma) and 1 µg of Spike-in antibody (Active Motif), were used. About 10 ng of immunoprecipated DNA (quantified with quantiFluor dsDNA system, Promega) were obtained at each time, and samples were submitted to EMBL-GeneCore Heidelberg for sequencing, that was performed by Illumina's NextSeq 500 technology (single-end, 85 bp reads). The quality of each raw sequencing file was verified with FastQC.