PacBio IsoSeq of C. closterium UGA8 from the inner shelf of the South Atlantic Bight: 24hr iron amendment
Cultures were filtered onto a 3 m polycarbonate filter, and a Qiagen RNeasy Plant Mini kit was used to extract RNA following the manufacturer's protocol, modified to include glass bead disruption.  Sequencing was performed at the Georgia Genomics and Bioinformatics Core (GGBC) at the University of Georgia with PacBio IsoSeq on a Sequel II SMRTCell