GSM7312259: P1-SCAF2962_2_HK, HCT116, THP1 and Jurkat T, Heat Killed, 10x genomics 3' v3; Homo sapiens; RNA-Seq
Single cell suspensions were prepared, and concentration and viability measured on an automated dual-fluorescence cell counter with acridine orange and propidium iodide stain (Luna Fx7, Logos Biosystems). Single cell partitioning and RNA-Seq library preparation was performed using 10X genomics 3' v3.1 chemistry (user guide CG000204) according to vendor recommendations. Sample viability was above 80% for all conditions. 6,000-8,000 cells were targeted to be captured for each sample. Libraries were sequenced on the NovaSeq 6000 with a target depth of 50,000 reads per cell using read parameters recommended by 10x Genomics user guides.