GSM7593270: WT Treg, with stimulation, repeat 2; Mus musculus; ATAC-seq
Cells were fixed, quenched and lysed to release chromatin DNA Tn5-based TruePrep Tagment Enzyme Mix (TTEMix) contains transposase and two kinds of adapters (Adapter 1 and Adapter 2) with equal molar. Input DNA are fragmented and linked with adapters on both ends just by mixing with TTE Mix, followed by a 10-minute incubation at 55°C.