GSM7311876: C. virginica, 2'3'-cUA, 6h, rep 4; Crassostrea virginica; RNA-Seq
Gill tissues were preserved in RNAlater and total RNA extraction was performed by Novogene Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library.