GSM7312500: Caco-2/15-Z1flag Postconfluent cells Exp2; Homo sapiens; RNA-Seq
We subjected the four samples of each (Sc or Pc) condition to strand-specific RNA-Seq method, relying on UTP incorporation in the second cDNA strand, as we previously described (Lamaa et al., 2020). Briefly, for each sample, 5–10 mg of total RNA, was extracted and submitted to EMBL-GeneCore (Heidelberg, Germany) for paired-end sequencing performed by Illumina's NextSeq 500 technology. Two replicates of each sample were sequenced. The quality of each raw sequencing file (fastq) was checked with FastQC. Stranded rRNA minus RNA-seq