TCR-seq of homo sapiens
Pre-treatment peripheral blood was collected into K2 EDTA Blood Collection tubes (Jiangsu Yuli Medical Equipment Co., Ltd., China) and stored at -80 C until further analyses. High-quality DNA was extracted using a QIAamp DNA Blood Maxi Kit (Qiagen, Hilden, Germany). The DNA concentration was measured using a Nanophotometer NP80 (Implen, Westlake Village, CA, USA), and the CDR3 gene fragments of the TCR chain were amplified using multiplex PCR. The amplified products were purified with a QIAEX II gel extraction kit (Qiagen) and used for TCR -chain sequencing libraries with a NEBNext Ultra DNA Library Prep Kit, according to the manufacturers instructions (NEB). TCR -chain libraries were sequenced on an Illumina NextSeq500 platform (Illumina, San Diego, CA, USA).