GSM8101899: 293T, DIS3-sh2+DIS3 WT, fraction 8, rep1; Homo sapiens; OTHER
RNA from fractions in sucrose density gradient centrifugation assay were extracted using TRIzol reagent (Invitrogen), and then used for the construction of  sequencing library. RNAs in each fraction were processed with KAPA RiboErase Kit (HMR) (KR1142) to deplete most ribosomal RNAs. Then, the ribo– RNA libraries for sequencing were prepared with KAPA RNA HyperPrep Kit (KR1350). These libraries were sequenced with NovaSeq 6000 at Sequanta Technologies, Shanghai, China.FastQC was applied for raw read qualities assessment. ribo- RNA-seq