GSM8012680: Control, protons, organoids, 2 days, mouse 1; Mus musculus; RNA-Seq
For the cDNA library preparation, total RNA was extracted from organoids at 2 and 6 days post irradiation using Rneasy Mini Kit according to the manufacturer`s instructions and its quality was determined using High Sensitivity RNA ScreenTapes on an Agilent 2100 Bioanalyzer. 100 ng of RNA was used as an input for all samples. 100 ng of highly intact RNA (RIN values > 9) was processed for the cDNA library preparation using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit following the manufacturers protocol.