GSM8011663: A. millepora adult 2; Acropora millepora; Bisulfite-Seq
DNA was extracted from adult corals and from individual and pooled larvae (approximately 10 larvae per pool).  Samples were lysed through bead beating and 2-hour incubation with CTAB buffer at 65 °C. Subsequently, an organic DNA extraction is performed using phenol:chloroform:isoamyl alcohol (25:24:1). DNA was then cleaned using the Zymo Clean & Concentrate kit. Whole genome bisulfite (WGBS) preparation was only completed on adult coral samples and pooled larvae using the Zymo-Seq WGBS Library Kit.