GSM7429854: VP12-RNA; Homo sapiens; RNA-Seq
PBMCs were isolated using a Ficoll gradient, frozen in CellBanker (Takara), and stored in liquid nitrogen vapor phase, while plasma samples were stored at -80°C until use. Frozen PBMCs were thawed quickly in a water bath at 37 °C. Droplet-based single-cell RNAseq was performed according to the protocol of the Chromium Single Cell V(D)J Reagent Kits v1.1 (10x Genomics). Gene expression, TCR and BCR V(D)J libraries were prepared for each sample according to the manufacturer's protocol (10x Genomics).