title: HIC_nave_WT1
design: Hi-C was performed from neuronal nuclei isolated from mouse sciatic dorsal root ganglia 3 days after sciatic nerve crush (SNC) or sham  or in nave conditions from WT (INTACT-AdvillinCre)  and Rad21 KO (INTACT-Scc1flox/flox-AdvillinCre) mice, 14 days after tamoxifen injection.  Neuronal nuclei,  expressing nuclear membrane GFP, were immunoisolated via beads bound to anti-GFP and were crosslinked with 2% formaldehyde for 10 min at room temperature.  Nuclei were processed for Hi-C using ARIMA Hi-C Genomics kits (A510008). Briefly, chromatin was digested with multiple enzymes, and purified proximity-ligated DNA was sheared using Bioruptor to obtain an average size of 400 bp. After DNA size selection of 200-600 bp, biotin enrichment, end-repair and adapter ligation, Hi-C libraries were prepared starting from 30-65 ng of DNA and 10 PCR cycles, using Swift Biosciences Accel-NGS 2S Plus DNA library kit (21024) and KAPA Library amplification kit (KK2620) or Arima Library preparation module (A303010).