title: 20w_lpr_2
design: sequencing libraries were generated and index codes were added by VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (NR604-01/02). Poly-T oligo-attached magnetic beads were used to purify mRNA from total RNA and double stranded cDNA was synthesized, with a tail attached to the end and connected to the sequencing connector. After selection of fragment size, cDNA library was produced by PCR enrichment and effective concentration was examined by Bio-RAD CFX 96 fluorescence quantitative PCR instrument. The cluster generation and sequencing were performed on Novaseq 6000 S4 Platform, using NovaSeq 6000 S4 Reagent kit V1.5