title: GBS of Rumex obtusifolius: adult leaf
design: DNA was extracted from freeze-dried samples using a modified CTAB method with the addition of polyvinylpyrrolidone-10 to help remove excess carbohydrates. DNA samples were digested with PstI restriction enzyme and uniquely barcoded adapters were ligated to the fragments. Subsequently, all samples were pooled into multiplexed libraries, each consisting of 94 samples. Libraries were cleaned and run through PCR before size selection (193-318 bp) with Pippin Prep.