title: GSM7849677: MDA-MB-231 shKDM2B 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from MDA-MB-231 Empty Vector and shKDM2B cells (50-70% confluence) using the Total RNA Purification Plus Kit (Norgen Biotek Corp., Cat. 48300). RNA purity and integrity were confirmed using the Agilent ScreenTape (Agilent Technologies Cat 5067-5576) before the preparation of the RNA-Seq libraries. Three biological replicates were used for library preparation. Libraries were prepared, using a KAPA RNA HyperPrep kit with RiboErase (Roche Cat KK8560)