title: Long-read sequencing of Citrullus amarus PI 299379
design: Using the covaris G-tube we generated 20Kb fragments by shearing genomic DNA according to the manufacturers recommended protocol. Using the AMpureXP bead purification system to remove the small fragments. Using the BluePippin Size selection system we remove the small fragmens for large-insert library. 600 minute movies were captured for each SMRT cell using the Sequel sequencing platform.