title: A primate nigrostriatal atlas of neuronal vulnerability and resilience in a model of Parkinson's disease
design: A primate nigrostriatal atlas of neuronal vulnerability and resilience in a model of Parkinson's disease
library_construction_protocol: macaque monkeys (6–18 years old) were used. Seven to nine sections were combined from one individual for nucleus isolation. The isolation protocol mainly comes from the published 10x Genomics protocol for nucleus isolation. In brief, the tissue was lysed via a chilled lysis kit (Sigma, no. NUC101-1KIT ). The suspension was then filtered with cell strainer and washed impurities by centrifugation with different concentrations of iodixanol. Nuclei-pellets were resuspended with a “resuspension buffer” which contained 1× phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA) and 0.2 U/μl RNase inhibitor. Nuclei-pellets were suspended in the DAPI solution and incubated for inspection under the microscope. NA NA MPTP (3mg; Sigma-Aldrich, St. Louis, MO, USA) was dissolved into a concentration of 0.15mg/ml and injected singly into the left carotid artery. NA 10X single cell isolation protocol 10X Genomics Chromium platform