title: GWAS of male Sheeppoodle
design: WGS data was obtained from DNA isolated from a whole blood sample of a male Old German Sheeppoodle. DNA library preparations and sequencing were conducted as follows: The genomic DNA was quantified using the Qubit 4.0 Fluorometer and qualified using the Agilent 5600 Fragment Analyzer. NEBNext UltraTM II DNA Library Preparation, Illumina clustering and sequencing were carried out following the manufacturers recommendations throughout the process. Briefly, 400~ng genomic DNA were fragmented by acoustic shearing with a Covaris instrument. Fragmented DNA was end repaired and adenylated. Adapters were ligated after adenylation of the 3ends followed by enrichment by limited cycle PCR. The DNA libraries have been validated using the Agilent 5600 Fragment Analyzer and quantified by using the Qubit 4.0 Fluorometer. The libraries have been multiplexed on a flowcell and loaded on the Illumina NovaSeq 6000 instrument according to the manufacturer's instructions. The samples were sequenced using a 2x150 paired-end configuration. Image analysis and base calling was conducted by the NovaSeq Control Software on the NovaSeq instrument.