title: genomic DNA from pBAD empty E. coli MG1655 infected with phage P1
design: Genomic DNA was extracted from E. coli cells aliquots according to a published protocol. Preparation of genomic DNA libraries was carried out using the NEBNext Ultra II FS DNA Library Prep Kit (NEB) according to the manufacturers instructions. The approximate insert size was selected to be in the range of 150 to 250 bp. Barcodes were introduced to both ends during library amplification step with NEBNext multiplex oligos for Illumina (NEB). Genomic DNA libraries were sequenced using HiSeq2500 platform (Illumina) (200-nt single-end reads).