title: T6A clinical isolate P592
design: Pneumococci were grown statically in tryptic soy (TS) broth (Becton, Dickinson) at 37C. Upon reaching the desired optical density of 1.0 at 620nm, cells were washed and diluted in sterile phosphate-buffered saline (PBS) for inoculation. Colonies expressing different amounts of CPS colonies were selected by visual screening using microscopy with oblique, transmitted illumination.