title: transcriptomic sequencing, brain, Atypus karschi
design: Total RNA was extracted using the RNAsimple Total RNA kit (TIANGEN, Beijing, China). PE 150 bp libraries were prepared and sequenced on an Illumina NovaSeq 6000 platform with an insert size of 250-300 bp. Raw sequences were filtered using FASTP, with ~6 Gb for each sample.