title: GSM7317513: H9 ESCs, wild type (WT), biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: For hESCs, RNA was harvested using the miRNeasy® Mini Kit (QIAGEN, Catalog #1038703) following manufacturer's protocol with minor modifications, including homogenization with QIAshredder (QIAGEN, Catalog #1011711), addtional chloroform extration step, and on-column DNase treatment using RNase-Free DNase Set (QIAGEN, Catalog #79254). For hESC-derived neurons, RNA was harvested using the Direct-zol RNA Miniprep kit (Zymo Research, Cat No. R2050) following manufacturer's protocols. For both hESCs and hESC-derived neurons, 1 ug of RNA was used for the construction of sequencing libraries. For hESCs, RNA libraries for RNA-seq were prepared using the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina® (NEB, Catalog #E7760L) following manufacturer's protocol for use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490). For hESC-derived neurons, RNA libraries for RNA-seq were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina, Cat No. 20020594 ) following manufacturer's protocols.