title: hard1_R1_001.fastq
design: Total RNA was extracted using RNeasy Mini Kit (Cat#74106, QIAGEN, HILDEN, GERMANY,) following the manufacturers instruction. Two samples for each group, 12 samples in total were collected. Libraries were prepared from 100 ng of total RNA using the following kits, NEBNext Poly(A) mRNA Magnetic Isolation Module (Catalog # E7490, NEW ENGLAND BIOLABS, MASSACHUSETTS, UNITED STATES), NEBNext Ultra II Directional RNA Library Prep Kit (Catalog # E7760, NEW ENGLAND BIOLABS,MASSACHUSETTS, UNITED STATES), NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (Catalog # E6440, NEW ENGLAND BIOLABS,MASSACHUSETTS, UNITED STATES) following the manufacturer's instructions.