title: RNASeq of Ciona intsetinalis st12 (mid gastrula)
design: RNA was harvested using Rneasy kit (Qiagen). 50 ng of total-RNA was used for the construction of sequencing libraries. RNA libraries for RNA-Seq were prepared using the PCR-cDNA Barcoding Kit from Oxford Nanopore Technologies (ONT) following manufacturer's protocols.