title: replicate 1 of smRNA-seq in WT
design: For each biological replicates, 5 ug of total RNA from five-day-old wild type (w1118, three replicates) control  female guts were extracted and ran with 15% urea denaturing polyacrylamide gel to purify 18-30nt small RNAs. These small RNA were then used as input to the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7300) with some modifications (1L of 10 M 2S rRNA block oligo 5 -TACAACCCTCAACCATATGTAGTCCAAGCA/3SpC3/-3 was added to each replicate before 5 SR adaptor ligation to eliminate rRNAs in final library)