title: GSM6704286: Brain coronal slice (1.5 mm posterior to the bregma) transcriptome [ZT14_M_Saline]; Mus musculus; OTHER
library_construction_protocol: Tissue preparation: 10X Genomics protocol CG000240-Rev C Slice staining: 10X Genomics protocol CG000160-Rev A (Hematoxylin-Eosin staining) 10X Genomics protocol CG000239-Rev D Paired-end dual indexed RNA sequencing with Illumina NovaSeq 6000 (Genome Quebec Innovation Centre, Montreal, Canada), at a sequencing depth of approximately 150M read pairs per sample. RNAseq was performed according to instructions of 10x Genomics for the Visium Spatial Gene Expression kit: read 1, 28 cycles; i7 index read, 10 cycles; i5 index read, 10 cycles; read 2, 90 cycles.