title: 16S sequencing (PacBio) of human fecal sample 1
design: A PCR mix of Platinum Superfi DNA polymerase master mix (Invitrogen), 0.4 M of both forward and reverse primers and 10 ng of template DNA was created individually for each PacBio sample. PCR conditions for the PacBio gyrB amplifications are as follows: 30 sec at 95 C; 30 cycles of 30 sec at 95 C, 30 sec at 57 C and 1 min at 72 C; then 5 min at 72 C.