title: MicroRNA profiling of rat left ventricles to investigate the hidden cardiac effects of rofecoxib in hypercholesterolemia
design: MicroRNA profiling of rat left ventricles to investigate the hidden cardiac effects of rofecoxib in hypercholesterolemia
library_construction_protocol: Lent ventricular samples were frozen in liquid nitrogen, then samples were powdered. Total RNA was extracted using Viogene miTotal RNA Extraction Miniprep System (Viogene Biotek, New Taipei City, Taiwan, R.O.C) according to the manufacturer's protocol.  For small RNA library construction, NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) was applied according to the manufacturer's protocol.