title: RNA seq of cortical mouse astrocytes Control 1
design: Total RNA from FACS isolated cells (astrocytes) was extracted with the Purelink RNA Micro kit (ThermoFisher). RNA was amplified with the MessageAmp II aRNA Amplification kit (ThermoFisher), which included a polyA selection.  Libraries from the amplified RNA were prepared using the Illumina Stranded mRNA kit (omitting the polyA tail pull down step). All samples were then multiplexed.