title: GSM8192312: RNA-seq, morula, Jarid2 KO, rep2; Mus musculus; RNA-Seq
library_construction_protocol: About 20 or single mourla embryo(s) per sample were pooled to generate each RNA-seq library. The zona pellucida was removed by brief incubation in acidic Tyrode's solution. All samples for RNA-seq were washed three times in 0.2% BSA/PBS before being transferred into a 0.2-ml tube with ~0.5μl carryover. RNA-seq libraries were prepared as previously described (Mei et al. 2021). Briefly, complementary DNA was prepared using a SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara; 634890) according to the manufacturer's instructions. Library construction was performed on a Nextera XT DNA Library Prep Kit (Illumina; FC-131-1024) with 200 pg complementary DNA, according to the manufacturer's instructions. All RNA-seq libraries were sequenced on a NextSeq 500 (bcl2fastq version 2.17 and RTA version 2.4.11) with single-end 75-bp reads (Illumina).