title: GSM7318560: leg muscle, male, biol rep3; Staurois parvus; RNA-Seq
library_construction_protocol: We completely disrupted the tissue using a homogenizer and extracted total RNA following the TRIzol protocol (Invitrogen). RNA precipitated overnight at -20 C. We pelleted the RNA by centrifugation, resuspended in 50 uL of nuclease-free water, and treated samples with TURBO DNAse (Invitrogen) to remove contaminating DNA. We cleaned up the RNA using 2x Ampure RNAclean beads (Beckman Coulter). We used 100 ng of total RNA to generate CNDA libraries using the Illumina Stranded mRNA ligation kit according to the manufacturer's protocol.