title: RNA-Seq of Acacia ligulata
design: Total RNA was isolated from leaves of a seedling of Acacia ligulata grown in Kings Park, Perth, Australia, as previously described [williams2015complete]. Extracted RNA was depleted of rRNA and sequenced with Illumina technology. Library preparation was performed with 300 ng of total RNA using the TruSeq Stranded Total RNA LT sample preparation kit and Plant rRNA Removal Mix (Illumina, San Diego, USA), following the manufacturers directions. The library was clustered on a Rapid Flow Cell v2 (Illumina), using the HiSeq Rapid PE Cluster Kit v2 (Illumina) and on-instrument cluster generation on the HiSeq1500 platform, and sequenced using the HiSeq Rapid SBS Kit v2.