title: GSM7027259: DFAM-22359 derived PDX, IPI-549, biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Mouse MLL-AF9+ L-GMPs were sorted from primary transplantation recipients. Lin-CD34+CD38- PDX cells were sorted from PDX mouse models upon vehilce or IPI-549 treatment in vivo. Sorted L-GMP cells or  Lin-CD34+CD38- PDX cells were homogenized in 1 mL of TRIZOL reagent and 0.2 mL of chloroform was added to the samples. Samples were vortexed vigorously for 15 seconds and then incubated at 4℃ for 10 minutes. After centrifugation at 12, 000 g for 15 minutes at 4℃, upper aqueous phase of the samples was carefully removed to a new tube and the total RNA was purified by RNAeasy Mini Kit (QIAGEN) according to the manufacturer's instruction. RNA libraries were prepared using Ampliseq Library Prep Kit following manufacturer's protocols for targeted amplicon amplification. Ion AmpliSeq Transcriptome Mouse Gene Expression Manual Chef-S5; Ion AmpliSeq Transcriptome Human Gene Expression Panel