title: YS leaf, three-year-old, developmental stage 1,rep1
design: The experimental materials consisted of three-year-old cuttings of leaf color mutant Whoa Nellie (YS) and its wild type Nellie R. Stevens (WS). Leaves representing stages 1 through 5 were collected at noon on sunny days every 7 days from April 19, 2024, to May 17, 2024 (exact sampling dates provided in Table S1), frozen in liquid nitrogen, and stored at -80 for transcriptome sequencing (2 cultivars  3 biological replications  5 stages). Fresh leaves were utilized for the measurement of physiological indicators (2 cultivars  3 biological replications  5 stages).  Total RNA was isolated from five developmental stages of green leaves from the wild type (WS) and yellow leaves from the yellow type (YS) using the NEBNext Ultra Plant RNA Extraction Kit (TaKaRa, Dalian, China). Three biological replicates were analyzed for each RNA sample.