title: Exome Sequencing of XP-C complemented human fibroblasts irradiated with 60 kJ/m2 of UVA
design: A two-step cloning strategy was established and improved by our group to detect mutations induced on the first/second round of replication. The first cloning step was carried out to homogenize the population's genetic background and obtain parental clones. The second cloning step was carried out from one parental clone selected in the first step, which was UVA irradiated (60 kJ/m2) or not and then subclones were selected and sequenced. Library preparation and exon capture were performed using the Agilents SureSelect QXT Human All Exon v6 kit, IDT xGen Hybridization capture of DNA libraries or Illumina Nextera Rapid Capture Exome starting from 5 g of genomic DNA. Libraries were sequenced in paired-end mode on the Illumina NextSeq 550 or HiSeq 2500 platforms.