title: ZTPSN20FD809-p5
design: Fresh samples were immediately extracted for bacterial genomic DNA using a faecal DNA extraction kit (MP Biomedicals, USA) and stored at -80C according to the manufacturer's protocol. The V3-V4 region of the bacterial 16S rRNA gene was then amplified using the forward primer 338F (5'-ACTCCTACGGGAGGCAGCA-3') and the reverse primer 806R (5'-GGACTACHVGGTWTCTAAT-3'). PCR amplicons were purified using Agencourt AMPure beads (Beckman Coulter, Indianapolis, IN) and quantified using the PicoGreen dsDNA detection kit (Invitrogen). PCR products were then subjected to high-throughput pyrosequencing on an Illumina MiSeq platform at Shanghai Personal Biotechnology Co.