title: scRNA-seq of HUVECs & LF
design: Based on the protocol from Carrion et al.s work, nattokinase powder was dissolved in the PBS in concentration of 100FU and was warmed enough in 37 water bath. The solution was centrifuged and filtered with 0.2m pore-sized filter before treatment in the microfluidic chip. After washing the samples in microfluidic chips with PBS, the nattokinase solution was treated directly to fibrin hydrogel through medium reservoir for 20 minutes. Completely detached cells were harvested from the microfluidic chips and stored in EGM-2 at 4, ready to be processed for scRNA-seq immediately.