title: Hi_GRIL_seq of A. baumannii AB5075.
design: The ribodepleted RNA samples were first fragmented using ultrasound (4 pulses of 30 s each at 4C). Then, an oligonucleotide adapter was ligated to the 3' end of the RNA molecules. First-strand cDNA synthesis was performed using M-MLV reverse transcriptase and the 3 adapter as primer. The firststrand cDNA was purified and the 5' Illumina TruSeq sequencing adapter was ligated to the 3' end of the antisense cDNA. The resulting cDNA was PCR-amplified to about 10-20 ng/l using a high fidelity DNA polymerase. The TruSeq barcode sequences which are part of the 5' TruSeq sequencing adapter are included in Table 2. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis.