title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq experiment of INA-6 multiple myeloma cells under different treatment
design: RNA-seq experiment of INA-6 multiple myeloma cells under different treatment
library_construction_protocol: The MM authenticated cell line INA-6 were selected for UNC1999 treatment. 100,000 cells/mL was seeded into flasks. 24h post seeding 1 µM of UNC1999 or DMSO (Sigma-Aldrich; cat. No 317275) was added to the cells. Media, supplements, and drug were renewed every 3rd day until end point and day 5. All experiments were conducted in 3 biological triplicates. RNA extraction was done by utilizing RNeasy Micro and/or Mini Kit (Netherlands, Qiagen; cat. no 74004/74104). 1 µg of total RNA was used for sequencing library preparation using TruSeq stranded total RNA library preparation kit with ribosomal depletion using RiboZero Gold (Illumina Inc.).