title: Whole Genome Sequencing of Opsanus beta: adult male blood
design: blood sample was extracted from adult male gulf toad fish (Opsanus beta), treated with citrate-dextrose (ACD) anticoagulant, and sent to the University of California Davis Genome Center DNA technologies and Expression Analysis Core Laboratory for High Molecular Weight DNA extraction, PacBio SMRT library preparation, and HiFi long read sequencing on a PacBio Sequel II. To get sufficient genome coverage, the library was sequenced on 3 SMRT-cells (runs arbitrarily labeled A,B, and C). For SRA upload, subread bams were split in half before submission (labeled 1of2 and 2of2 respectively).