title: GSM6704062: Tuft cells, B6, untreated [UG10]; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted directly into buffer from SMART-Seq4 and cDNA was prepared according to manufacturers protocol Libraries were prepared using NexteraXT DNA Library kit according to manufacturer's protocol Barcoded libraries were pooled and quantified using a Qubit® Fluorometer (Life Technologies). Single-read sequencing of the pooled libraries was carried out on a HiSeq2500 sequencer (Illumina) for 74 cycles, using TruSeq v3, and SBS kits (Illumina). Target read depths were ~5-10 million raw reads per sample.