title: Ileum- Microbiome of Mus musculus: Male Fe-Enriched LFLS  diet
design: Intestinal luminal contents were lysed using bead beating (0.1-mm silica beads). Samples were lysed with the OMNI Bead Ruptor 12 (Precellys) 2 cycles of 1 minute. Bbefore enzymatic digestion with 50 mg of lysozyme and 200 U/L mutanolysin (37C, 45 min). Microbial DNA was extracted using the QIAamp DNA Stool minikit (Qiagen, CA, USA), and amplification of the V3-V4 region was performed using the primers Bact-0341-b-S-17 (5-CCTACGGGNGGCWGCAG-3) and Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC-3) (Illumina, CA, USA). Libraries were purified using magnetic beads AMPURE XP (Beckman Coulter Canada Lp), and libraries were assessed on gel using QIAexcel (Qiagen, CA, USA). High-throughput sequencing (2- by 300-bp paired end) was performed on a MiSeq platform (Illumina, CA, USA).