title: Input of BM cells: Adh5Aldh2 1st
design: Lineage-depleted mouse bone marrow cells using Direct Lineage Cell Depletion Kit (Miltenyi Biotec) were lysed in SDS buffer (2% SDS; 20 mM Tris, pH 7.5) followed by sonication using Covaris M220 system (5% duty factor, 200 cycles per burst, 16 min at 25C in microTUBE). The sonicated samples were incubated in KCl buffer (200 mM KCl; 20 mM Tris, pH 7.5) with 0.04 mg/ml Proteinase K at 55C for 3 h. After centrifugation at 20,000 g for 5 min at 4C, the supernatant was purified using MinElute PCR Purification Kit (QIAGEN). Purified DNA was subjected to preparation of next-generation sequencing libraries using MGIEasy Universal DNA Library Prep Set (MGI). The library quality and quantity were verified by Multina (SHIMADZU) or 4150 TapeStation (Agilent).