title: RNA-seq_Unio_elongatulus
design: For each tissue total RNA was extracted using the NZY Total RNA Isolation Kit (NZYTech, Lda. - Genes and Enzymes), following the manufacturers guidelines. RNA concentration (ng/l) and quality measurement (OD260/280 ratio values) were obtained using a DS-11 Series spectrophotometer / fluorometer. RNA extractions from the eight tissues were sent to Macrogen, Inc., where strand-specific libraries were built (insert size of 250300 bp) and total RNA sequenced using 150 bp paired-end reads on the Illumina HiSeq4000 platform.