title: PMseq-DNA of sputum/BALF
design: RNA was extracted and enriched following the manufacturers operational manual, using TIANMicrobe magnetic beads method pathogenic microorganism DNA/RNA extraction kit (NG550-01). After revers transcrption and two-strand synthesis, DNA libraries were constructed through DNA-fragmentation, end-repair, adapter-ligation and PCR amplification. Agilent 2100 was used for quality control of the DNA libraries. Quality qualified libraries were pooled, DNA Nanoball (DNB) was made and sequenced by BGISEQ-50 /MGISEQ-2000 platform.