title: metabarcoding of fisheries samples: eDNA in catch water to estimate species composition
design: Short and long region amplicons of the mitochondrion genome targeting 12S and 16S rRNA genes and the cytochrome c oxidase subunit I gene in fish and metazoan were amplified. After purifying the PCR products, fragments were mixed in equimolar ratios for each sample. A library was prepared using the Nanopore Amplicons by Ligation (SQK-LSK110) sequencing kit, according to manufacturers instruction, for each sample separately. Sequencing was performed on a Flongle flow cell (FLO-FLG001) separately for each sample. Following the protocol recommendation, 20 fmol of the final library was loaded onto the flow cell and the sequencing run started, enabling live basecalling using Guppy with the High accuracy (HAC) model.