title: Human stool metagenome: Adenomas
design: DNA was extracted from _x0003_100 mg of stool using enzymatic treatment with 15 mg/ml lysozyme and proteinase K (Qiagen) for 10 min incubation followed by mechanical lysis using 0.1mm glass beads and 3 min bead beating on the homogenize setting of a Mini Bead beater-8 (Biospec Products). After lysis, samples were centrifuged for 5 minutes at 30,000 rpm to pellet debris and supernatant passed through a QIAshredder spin column (Qiagen) prior to proceeding with nucleic acid extraction using a Allprep DNA/RNA Mini Kit (Qiagen) following manufacturer protocol. Metagenomic libraries were constructed from 100250 pg of DNA using the Nextera XT DNA Library Preparation Kit (Illumina) as per the manufacturers recommended protocol. Sequencing was performed on the HiSeq 2500 2x 101 PE platform (Illumina), targeting _x0003_2.5 Gb of sequence per aliquot. Demultiplexing, BAM and FASTQ file generation were performed using the Picard suite (https://broadinstitute.github.io/picard)