title: Bacterial 16S rDNA sequencing of OSCC sample
design: DNA was extracted from all samples using DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The concentration and purity of the isolated DNA were detected using 1% agarose gels electrophoresis. DNA was diluted to 1ng/l using sterile water. Genome DNA from each sample was used as an amplification template. PCR targeting of the V3-V4 region of the 68S rDNA hypervariable region was conducted