title: RNA-Seq of mus musculus
design: LLC cells in the logarithmic growth phase were digested with trypsin, and the cell suspension was stained with trypan blue cell viability dye for counting. After counting, the corresponding phosphate buffer was added to prepare a cell suspension with a concentration of 5  106 cells. The hair under the right armpit of the mice was removed and disinfected with an iodine cotton ball. Each mouse was inoculated with 5  105 LLC cells. After 4-5 days of inoculation, a clear boundary, regular shape, hard quality, and poor activity mass could be touched at the inoculation site, indicating that the modeling was successful. The LLC tumor-bearing mice were randomly divided into two groups (n = 6 mice/group): control and YQ groups. Approximately 10 g of tumor tissue was cut, and total RNA was extracted after TRIzol lysis. The library was constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420, Northeastern University, USA) and sequenced on a NextSeq500 (Illumina, USA). RNA-seq raw data were mapped to the reference genome (hg19) using TopHat2.16 cufflinks to characterize differential transcription patterns.