title: GSM6600970: Alignment_M1; Mus musculus; RNA-Seq
library_construction_protocol: In brief  the total RNA was isolated from blood sample using TRIzol reagent (Invitrogen  Carlsbad  CA  USA) based on the manufacturer's instructions. Before the sequencing experiment  the integrity and quantity of each RNA sample were checked using agarose gel electrophoresis and the NanoDrop ND-1000 (NanoDrop  Wilmington  DE  USA) instrument. cDNA was synthesized and amplified using Illumina's proprietary reverse transcription primers and amplification primers. Subsequently  ~134-160 bp PCR amplified fragments were extracted and purified from the PAGE gel. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100. We use the commercial kit for tRF & tiRNA-seq library preparation  which includes 3' -adapter and 5' -adapter ligation adaptor ligation  cDNA synthesis and library PCR amplification. The prepared tRF & tiRNA-seq libraries are finally quantified using Agilent BioAnalyzer 2100  then sequenced using Illumina NextSeq 500.