title: RNA-sequencing-mock-replicate2
design: Arabidopsis seedlings were grown on 1/2 Murashige and Skoog (MS) plates containing 0.8% agar and 1% sucrose at 22 C under 12/12 h light/dark cycles with 55% relative humidity. For elf18 treatment, Arabidopsis seedlings were grown on plates for 7 days and transferred to liquid 1/2 MS solution and grown for one more day (8-day-old) before treated with 10 mM elf18 or water for one hour. Arabidopsis seedlings treated with elf18 or water were collected, frozen in liquid nitrogen, and ground using the Genogrinder (SPEX SamplePrep). The ground tissue was homogenized in Trizol (Invitrogen) and subject to total RNA isolation using Direct-zol RNA Miniprep Plus Kit (Zymo). Library preparation was performed using KAPA Stranded RNA-Seq Library Preparation Kit (Roche). The six libraries for RNA-seq were pooled at equal amount of DNA and subject to the next-generation sequencing using Illumina NovaSeq with pair-end 50 bp.