title: GSM7323515: PBMC, CAP Patient 8, 4 weeks post-enrollment; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs were isolated from whole blood using Ficoll gradient and frozen in -80C. Frozen PBMC aliquots were thawed dropwise using 37C T-cell media. After thawing, PBMC were then incubated at 37C and 5% CO2 for 24 hours in tissue-culture treated 96 well plates in 250 uL of T-cell media. RNA was isolated using the Arcturus PicoPure RNA Isolation Kit per protocol (Life Technologies) Libraries were poly-A enriched and constructed using TruSeq PolyA kits