title: GSM7324504: aboveground plant parts, inoculated with Williamsia sp. Leaf354 at 10^6 cfu/ml, biol. rep. 1, exp.2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: The plant material was collected in RNase-free microcentrifuge tubes (MCT-150-C, Axygen, Corning, USA) and was then immediately flash frozen in liquid N2. Two plants from different growth boxes were pooled per tube to form a biological replicate. Three biological replicates were sampled per treatment and time point. Flash-frozen samples were ground to a fine powder in the collection tube using Teflon pestles (General Lab Supply, Lab Supply, Dunedin, New Zealand). RNA extraction was performed using the Isolate II RNA Plant kit (Bioline, London, England). RNA library preparation and sequencing was performed by Custom Science (Epping, Australia). RNA quality and purity was assessed using a Nanodrop (ND-2000 Spectrophotometer, Thermo Scientific, Waltham, MA, USA). RNA integrity was verified on a 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA) using the RNA 6000 Nano Kit (Agilent). Poly-A enriched libraries were prepared using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, USA) for Illumina. RNA sequencing was performed on a NovaSeq 6000 (Illumina, San Diego, USA) platform. Approximately 30,000,000 paired-end reads with a length of 150 bp were generated per sample.