title: Geographical Distribution of Mucosa-Associated Gut Microbiome in Children with Autism
design: Genomic DNA was isolated from GI biopsies using a MagMAX Microbiome Ultra Nucleic Acid Isolation Kit and KingFisher Apex Instrument. DNA was prepared for 16S rRNA gene sequencing using a Zymo Research Quick-16S Plus NGS Library Kit (v4).DNA libraries were sequenced using a NextSeq 1000 sequencer, generating 2x150-bp reads.