title: Epi-Decoder tDNA-Ty1 locus: wild type glycerol 2h IP
design: Samples were prepared as described in van Breugel et al. (2022). Briefly, TAP-tag ChIP was performed on library pools of barcoded and TAP-tagged yeast strains grown in glucose and glycerol 37C (2h). To prepare libraries for sequencing, barcodes in each sample were amplified using unique index primers. Subsequently, PCR products were mixed in equimolar fashion and purified from a 2% agarose gel with a QIAquick gel extraction kit (Qiagen). Purified DNA was sequenced (single read, 65bp) on a HiSeq2500 platform (Illumina) using a custom sequencing primer (GCCCTTAACAACAATAATGAATATTGAGTGCATAATGTTGA). Samples are demultiplexed using the unique sample index primers. The Perl script eXtracting Counting and LInking to Barcode References (XCALIBR, https://github.com/NKI-GCF/xcalibr) was used for each indexed library to generate barcode count tables. Reference for barcodes are available on the study page.