title: Low pass whole genome sequencing of Homo sapiens
design: Flash-frozen tissue in DNA/RNA Shield was homogenized via a bullet blender. Genomic DNA was extracted using Zymo Quick DNA/RNA microprep plus kit (Zymo Research, D7005). Library preparation and low-pass Whole Genome Sequencing were carried out at TGen or by Gencove, Inc. At TGen, libraries were prepared using PCR-free Watchmaker DNA Library Prep Kit with Fragmentation5 (Watchmaker Genomics, 7K0019-096) with 200 ng input. Genomes were sequenced on NovaSeq at low coverage (typically 0.4–1x). The resulting sequence data were processed and imputed using Gencove's imputation platform.