title: GSM7679680: H3K36me2_control; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag was performed using NovoNGS CUT&Tag 3.0 High-Sensitivity Kit (for Illumina) (Novoprotein) according to manufacturer's instructions. In brief, LP-1 control and PSMD14 knockdown cells were collected and bounded to Concanavalin A–coated beads. Subsequently, cells were resuspended in antibody buffer and incubated with primary antibodies against PSMD14 or NSD2 and secondary antibodies in order. The samples were incubated with pA-Tn5 transposase. After transposon activation and tagmentation, DNA was isolated, amplified, and purified to construct library. The library for sequencing was constructed and NovoNGS DNA Clean beads were used for purification steps. The library was quantified with NovoNGS CUT&Tag 3.0 High-Sensitivity Kit (for Illumina) and sequenced on an Illumina novaseq 150PE.