title: GSM8417100: RNA-seq iPSC WT control rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from iPSCs plated for 48 h on Matrigel-coated 6 WP with the RNAeasy Micro Kit (Qiagen #74004) according to the manufacturer's protocol with the following modifications: after disrupting the cells with RLT buffer, cell suspension was transferred into a QIAshredder spin column (Qiagen #79656) and centrifuge. The lysate was pipetted directly, without further dilution, into a QIAshredder spin column and centrifuged for 2 minutes at full speed (13,000 rpm). The RNA concentration and purity were measured using SpectraMax QuickDrop UV-Vis Spectrophotometer (Molecular Devices, #7546). Libraries were prepared with the RNA Library Prep Kit (Illumina)