title: RNA-seq of FR4+Egr2+, FR4+Egr2-, FR4-Egr2-, FR4+Egr2-/-Egr3-/- and FR4-Egr2-/-Egr3-/- CD4 T cells
design: RNA-seq of FR4+Egr2+, FR4+Egr2-, FR4-Egr2-, FR4+Egr2-/-Egr3-/- and FR4-Egr2-/-Egr3-/- CD4 T cells
library_construction_protocol: CD4+CD25-CD44hiFR4-Egr2- cells were isolated from Egr2 Kin mice by FACS RNA was isolated and purified using the RNeasy UCP Micro Kit (Qiagen) according to the manufacturer's instructions RNA from T cells was processed for directional mRNA-Seq library construction using the KAPA mRNA HyperPrep Kit (Kapa Biosystems) in combination with IDT xGen UDI-UMI adapters (Integrated DNA Technologies) according to the manufacturersâ€™ protocols