title: GSM7324487: sham3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent.RNA quality and quantity was measured by using nanodrop spectrophotometer (ND-1000, Nanodrop Technologies) and RNA Integrity was determined by gel electrophoresis Libraries were prepared by using KAPA Stranded RNA-Seq Library Prep Kit(Illumina). Briefly,Total RNA of each sample was used to prepare the RNA sequencing library 1)RNA fragment;2)Random primed first strand cDNA synthesis;3)dUTP based second strand cDNA synthesis;4)Adaptor ligation and PCR amplification.