title: Amplicon seq of Water without L1: larvae 12 hr 7
design: Total DNA was extracted using DNeasy PowerSoil Kit. DNA samples were stored at 20C until PCR amplification and sequencing. Extracted DNA was quantitated using Qubit 2.0 Fluorometer (Thermo Scientific, Waltham, MA, USA) and standardized to a concentration of 5 ng/l.