title: GSM7679641: Iloprost_POST_219760_RUL; Homo sapiens; RNA-Seq
library_construction_protocol: During the initial training visit, a 2.5 µg test dose was administered and participants delivered the full 5 µg doses subsequently. All study participants were to self-administer 5 µg iloprost or placebo QID for 2 months. An initital bronchoscopy (Pre) and 2 monnths (post) treatment were performed. Frozen airway biopises collceted at pre and post treatment were analyzed. Bronchial biopsies frozen in OCT were used to purify total RNA using an RNA extraction microkit (Qiagen), followed by mRNA library prep (Illumina) and sequencing (NovaSEQ next-gen sequencer).