title: RNA-seq of Dipsacus asperoides
design: Five kinds of tissues (roots, leafs, flowers, stems, and lateral roots) were acquired from Dipsacus asperoides specimens. Following tissue dissection, samples were immediately snap frozen in liquid nitrogen. Total RNA was extracted using the Plant Total RNA Kit (MilliporeSigma). RNA-seq libraries for PacBio SMRT sequencing were prepared following the Iso-Seq Template Preparation for Sequel Systems protocol (PacBio). Sequencing of pooled, barcoded libraries was performed on a PacBio Sequel System instrument.