title: GSM7816087: Spinner flask day 2 control, replicate 2; Homo sapiens; OTHER
library_construction_protocol: Samples were PBS-washed and cell pellets frozen. Genomic DNA was extracted using the QIAamp DNA blood maxi kit (Qiagen). sgRNA libraries were prepared by PCR amplification to add sequencing adapters and indices. CRISPR/Cas9 knockout screen based on sgRNA locus sequencing