title: GSM7680511: GC, 0-20 hr, scATAC-seq, ATAC part; Drosophila melanogaster; ATAC-seq
library_construction_protocol: scRNA-seq: 1. Homogenization of 8-12 and 12-20 hr. embryos 2. Collect vas-GFP cells using Fluorescence-activated cell sorting scATAC-seq: 1. Homogenization of 0-4, 4-8, 8-12, 12-16 and 16-20 hr. embryos 2. Collect vas-GFP cells using Fluorescence-activated cell sorting 3. Nuclei isolation using 10 mM Tris-HCl (pH7.4) conatining 10 mM NaCl, 3 mM MgCl2, 1% BSA, 0.01% Tween-20, 1 mM DTT, 0.01% NP-40, 0.001% Digitonin and 1 U/μl RNase inhibitor The protocols were based on the manufacturer's instructions using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 for scRNA-seq and Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle for scATAC-seq