title: Whole Transcriptome Sequencing
design: MRNA was purified and fragmented from total RNA using poly-T oligo-attached magnetic beads using two rounds of purification. These cDNA fragments then had the addition of a single 'A' base and subsequent ligation of the adapter. RNA sequencing is performed using an Illumina NovaSeq 6000 system following provided protocols for 2x100 sequencing.