title: GSM6643249: PTPN2 -/- Rep 3 not treated; Mus musculus; RNA-Seq
library_construction_protocol: Islets from 3 control and 3 Ptpn2-bKO mice were treated with pro-inflammatory cytokines. Treated and untreated islets were washed 3 times with cold-PBS. Total RNA was extracted using the RNeasy Micro Kit (QIAGEN) following instructions. We confirmed the RNA Integrity Number (RIN) value using an Agilent 2100 Bioanalyzer. RNA samples all had RIN values over 8.0. Libraries were constructed using 0.5 µg total RNA. RNA was isolated with the RNeasy Micro kit (QIAGEN). Sequencing was performed using Illumina NovaSEQ6000.