title: Illumina NovaSeq 6000 sequencing; Comparative analysis of the Illumina Mouse Methylation BeadChip and Reduced Representation Bisulphite Sequencing for routine DNA methylation analysis of murine samples
design: Comparative analysis of the Illumina Mouse Methylation BeadChip and Reduced Representation Bisulphite Sequencing for routine DNA methylation analysis of murine samples
library_construction_protocol: Tissue samples were obtained from the proximal small intestine using a scalpel blade. DNA was extracted using the AllPrep DNA RNA Protein Minikin We generated single base resolution DNA methylation data using the Ovation RRBS Methyl-Seq System 1-16 (Tecan Life Sciences) with 100ng of input DNA. This approach employs the MspI restriction enzyme to digest DNA at the CCGG motif, which is highly enriched in CpG dense regions of the genome. This generates a library of small (>300bp) fragments that are rich in CpG content. Sequencing libraries are generated from these fragments, bisulphite converted and sequenced.