title: NGS of mixed algal-prokaryotic cell suspension from Reactor 2 (pretreated with wastewater), Experimental Run #2, day 10, replicate 3
design: Harvested cells were extracted for DNA using the DNeasy Plant Mini Kit(QIAGEN) according to the manufacturers instructions. Amplification and Illumina sequencing (MiSeq 300PE; paired-end) of extracted gDNA was carried out by McGill University at the Genome Quebec Innovation Centre. All amplicon sequences reads were generated using modified, staggered end primers 926F (AAACTYAAAKGAATWGRCGG) and 1392R (ACGGGCGGTGWGTRC), where 0-3 random bases were inserted between both primer and Illumina adaptor sequences.