title: Rat fecal microbiota: 16S rRNA gene sequencing
design: Libraries were prepared according to the manufacturer's protocol (Ligation sequencing amplicons) with modification. The prepared DNA library was loaded onto the R9.4 flow cell (FLO-MIN106; Oxford Nanopore Technologies), and sequenced on the MinION Mk1B. MINKNOW software ver. 22.12.7 (Oxford Nanopore Technologies) was used for data acquisition.