title: MT-silt
design: MG library was constructed by NEBNext Ultra DNA Library Prep Kit (NEB, Ipswich, MA, USA), while total RNA for MT library was extracted using RNeasy PouerSoil Total RNA Kit (Cat: 12866-25, QIAGEN) following the manufacturers instructions. Quality control of libraries was conducted by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Qualified DNA and total RNA were purified by Agencourt AMPure XP Beads (Cat: A63881, Beckman), while qualified total RNA was further purified by RNAClean XP Kit (Cat: A63987, Beckman Coulter, Inc.Kraemer Boulevard Brea, CA, USA) and RNase-Free DNase Set (Cat#79254, QIAGEN, GmBH, Germany).