title: plasma sample from hamster after the S. mansoni infection at week 12  incubated with the phage-display library following the PhIP-Seq protocol
design: DNA was amplified by two rounds of PCR using Platinum superFi PCR Master Mix. In the first round, 56 L reactions had 30 L of PhIP and 0.1 M of the 5'-GCAACACGatgaccatggcatccag-3 'and 5'-GCACCGGGTacgtactgcagacgatg-3' primers were used, which yearn part of the vector and part of the adapters that flank the DNA insert of the library. The thermal profile followed the manufacturer 2-steps PCR suggestions, and the denaturation and extension steps were repeated in 35 cycles. This reaction was purified using 1.2 X Agencourt AMPure XP, as suggested by the manufacturer. The second PCR product was purified with 0,65X Agencourt AMPure XP and paired-end-sequenced with Illumina HiSeqXTen.