title: sequences of bacteria
design: After the PEreads obtained by Illumina sequencing are sample split, the paired-end reads are first quality controlled and filtered according to the sequencing quality. At the same time, the paired-end reads are spliced according to the overlap relationship between the paired-end reads to obtain optimized data after quality control splicing. Then use sequence denoising methods (DADA7/Deblur, etc.) to process the optimized data to obtain ASV (AmpliconSequenceVariant) representative sequence and abundance information.