title: GSM7729996: APAP_6h_1 [miRNA]; Mus musculus; miRNA-Seq
library_construction_protocol: Liver tissue of C57 BL6 mice were treated with liquid nitrogen immediately and maintained at -80℃. 1 ug of total RNA was used for the construction of sequencing libraries. Using the Small RNA Sample Pre Kit, we constructed a library by leveraging the distinct structures at the 3' and 5' ends of Small RNA, which possess complete phosphate groups at the 5' end and hydroxyl groups at the 3' end. Starting with total RNA, the ends of Small RNA were directly ligated with connectors, followed by reverse transcription to synthesize cDNA. After PCR amplification, the target DNA fragment was isolated using PAGE gel electrophoresis. The cDNA library was then obtained through gel excision and recovery.