title: RNA-Seq of Cyprinus carpio: ovary
design: Equal amounts of RNA were taken from each of the 3 fish at the same time point and mixed to make a new sample. Three mixed samples were then selected for cDNA libraries construction at each time point. The cDNA libraries were constructed by Guangzhou Kidio Biotechnology Co., Ltd., using NEBNext Ultra RNA Library Prep Kit. The main experimental steps included mRNA enrichment using Oligo (dT) magnetic beads, random RNA disruption using fragmentation buffer, first-strand cDNA synthesis, second-strand cDNA synthesis and purification, end repair, addition of poly(A), ligation of Illumina sequencing adapters, purification using the AMPure XP Beads (1.0X) and PCR library amplification