title: Metagenome of groundwater AH_WF_B
design: Genomic DNA was extracted from sediment using the QIAGEN PowerMax Soil kit (QIAGEN, USA) according to the manufacturers instructions with some modifications. As a modification, after the addition of Solution C1 of the Soil Kit, the sample was subjected to a freeze_thaw and vortexed at max speed for 3.5 min after thawing. The samples were then incubated in a 65 C water bath for 30 min and mixed by inversion every 10 min. Incubation steps with Solution C2, C3, and C4 were extended to 30 minutes. DNA was eluted using 5 mL of Solution C6 which had been pre_warmed to 55 C. The filter was incubated for a minute, then the DNA was eluted by centrifugation. This elution was repeated again to improveyield. DNA was concentrated by ethanol precipitation, using 1/10 volume of sodium acetate solution (3 M, pH 5.2, with 0.4 mg/mL glycogen) and 2.5 volumes of chilled 100% ethanol. Sequencing libraries were prepared using the Illumina DNA Prep Kit (Illumina, USA)per manufacturers instructions. Samples were sequenced ousing 2 x 150 bp on the IlluminaHiSeq 4000 (Illumina, USA).