title: ITS rDNA sequencing of microbial structure
design: The universal primers were chosen. The PCR reactions were carried out by using Phusion High-Fidelity PCR Master Mix with GC Buffer (New England Biolabs, Ipswich, United States) and the high-fidelity thermostable DNA polymerase.. PCR products were monitored on 2% agarose gel. The sequencing of PCR products was performed on an IlluminaNovaSeq 6000 platform. The raw data were then subjected to a quality control procedure.