title: GSM8421738: Myotubes, Ad Flag-Zfp697, Flag ab, rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked by adding 1% formaldehyde directly into the media for 12 minutes at room temperature. Cell pellet was washed and resuspended in 0.5 mL of ChIP Lysis Buffer (1% SDS, 10 mM EDTA, 50 mM Tris-HCl pH 8.0) supplemented with protease inhibitor cocktail (Roche, EDTA-free) and homogenized. The chromatin was then submitted to 5 cycles of sonication (Bioruptor Plus, Diagenode; 30 seconds ON, 30 seconds OFF) at low intensity. The libraries for ChIP-seq were generated using the Takara ThruPLEX DNA-Seq Kit (Takara, R400736)