title: Shotgun metagenomics sequencing of in-situ microcosms in Hudson Bay
design: In-situ microcosms containing clay beads. The microcosms were incubated for one year in Hudson Bay, Nunavut. The DNA from the biofilm on the surface of the beads was recovered with a modified CTAB method and and libraries were prepared using the Nextera XT DNA Library Preparation Kit from Illumina. Products were pooled and sequenced on an Illumina NovaSeq on a 2 x 150 bp configuration.