title: Hi-C sequencing of Citrullus amarus PI 299379
design: A Hi-C library was prepared using the Dovetail Hi-C library preparation kit according to the manufacturer protocol. Briefly, nuclear chromatin was fixed in fish muscle tissue with formaldehyde and extracted. Fixed chromatin was digested with DpnII, and sticky ends were filled in with biotinylated nucleotides and ligated. Then, crosslinks were reversed, and purified DNA was treated to remove any free biotin from ligated fragments.