title: Nanopore sequencing of SARS-CoV-2 RNA standard
design: gDNA or cDNA samples were prepared for nanopore sequencing libraries by using the ligation method. In details, 400ng gDNA or cDNA were treated with NEB Next Ultra II End repair / dA-tailing Module (New England Biolabs, NEB, USA) and ligated using different barcodes in a native barcoding kit (Oxford Nanopore Technologies, ONT, UK) with NEB Blunt/TA Ligase Master Mix (NEB, USA). Then, the adapter from the ligation sequencing kit LSK109 (ONT, UK) was ligated using the NEBNext Quick Ligation Module (NEB, USA) at room temperature. The nanopore sequencing device MinION Mk1B (ONT, UK) was used for nanopore sequencing with the R9.4 flowcell.