title: NextSeq 500 sequencing; Ribosome profiling and RNA-Seq of arabidopsis seedling in two different temperatures
design: Ribosome profiling and RNA-Seq of arabidopsis seedling in two different temperatures
library_construction_protocol: 2 weeks old arabidopsis seedlings were cultured in 17ºC and were either shifted to 27ºC or remain at 17ºC prior harvesting by snap freeze in liquid nitrogen liquid 1/2-MS media under constant light RNA were extracted by means of phenol-chloroform extraction. Adopted TrueSeq small RNA v2 for ribosome profiling and RNA-seq