title: GSM7101176: Control KPP biological rep1; Mus musculus; RNA-Seq
library_construction_protocol: TA muscles were snap frozen in either dry ice or liquid nitrogen and then homogenized in 1mL of Trizol. Libraries were prepared by MUSC Translational Core using the New England Biolabs NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat# 7490L) and Ultra II Directional RNA Library Prep Kit from Illumina (Cat# 7760L). Dual indexed libraries were pooled and sequenced at VANTAGE (Vanderbilt University Medical Center) on an Illumina NovaSeq 6000 to a depth of approximately 25 million paired-end 150 bp reads per library.