title: 16S sequencing (MiSeq) of human fecal sample 5
design: A PCR mix of Platinum Superfi DNA polymerase master mix (Invitrogen), 0.4 M of both forward and reverse primers and 10 ng of template DNA was created individually for each MiSeq sample. PCR conditions for the original MiSeq gyrB  amplifications are as follows: 2 mins at 98 C; 25 cycles of 10 sec 98 C, 20 sec 56.6 C, 15 sec 72 C; then 1 cycle of 5 min at 72 C