title: GSM6704382: (D) Emergency granulopoiesis neutrophils, after infection, rep 2; Danio rerio; RNA-Seq
library_construction_protocol: Larvae were homogenised in 0.25% trypsin with mechanical agitation prior to FACS-isolation of 1000 neutrophils per sample. cDNA was synthesised directly from cell lysates using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio) Libraries were made using the Nextera XT DNA Library Preparation Kit (Illumina).