title: GSM8193425: 7 day-old Nicotiana tabacum SR1 seedling, induced by estradiol and collected at 4h, Replicate 1; Nicotiana tabacum; RNA-Seq
library_construction_protocol: Tissues were frozen in liquid nitrogen, ground, and RNA was extracted using the Spectrum Plant Total RNA Kit with on-column DNase I digestion. The RNA-seq libraries were generated and sequenced by Genewiz (Azenta life science) using Illumina NovaSeq sequencing plateform. A minimum of 38 million 150 bp paired-end reads were obtained per sample