title: GSM7911156: H3K9me2_CUTRUN_KO3; Mus musculus; OTHER
library_construction_protocol: Tumor tissue was resected and digested with enzyme. Lymphocytes were purified using percoll. Treg cells were purified by magnetic-activated cell sorting (MACS) positive selection. Tumor infiltrated treg of the WT and jmjd1c tregcKO mice were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or absence of fluorence signal. H3K9me2 modified genomic regions and Stat3 binding regions were harvested by anti-H3K9me2 antibody anti-Stat3 antibody and “HD102 Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina”. Libraries were constructing using “HD102 Hyperactive pG-MNase CUT&RUN Assay Kit for Illumina” and “TD202 TruePrep® Index Kit V2 for Illumina®” from Vazyme Biotech Co., Ltd.