title: GSM6601931: sows liver (high bile acid group) IP; Sus scrofa; ChIP-Seq
library_construction_protocol: One hundred million cells  were fixed with 1% formaldehyde and sonicated for 8 min (10s on and 15s off) on ice  with a 2-mm microtip with a 40% output control and 90% duty cycle setting. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific. 10 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter.