title: RNAseq of strawberry fruit shelflife
design: Fragaria ananassa cv. Elsanta strawberry fruit at red rip stage were washed with water, the calyx was removed using a sharp blade, and they were immersed in 200 ppm sodium hypochlorite for 2 min. They were then air dried in a laminar air flow hood and halved. Halved fruit were stored at 8C and sampled for RNA extraction at 0, 1, and 5 days of storage. RNA was extracted according to Greco et al. (2014) https://doi.org/10.1371/journal.pone.0096470. RNA integrity and quantity were  assessed using an Agilent 2100 bioanalyzer-RNA 6000 Nano Chip (Agilent Technologies) and a Qubit fluorometer. Random primed cDNA libraries were prepared using a TruSeq RNA Sample Prep kit (Illumina) and Illumina sequencing was performed using paired-end mode on an Illumina Next Seq 5000 Platform.