title: GSM7100222: RPB2_rep2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Exponentially growing cells were cross-linked with 1% formaldehyde for 15 minutes and chromatin lysated were prepared. Pulldown of cross-linked proteins was done using a TAP antibody. Sequencing libraries were made using the KAPA Hyper Prep Kit (KAPA Biosystems). Libraries were amplified by PCR and size selection (200-450 bp) was performed using AMPure XP beads (Beckman Coultier). DNA concentration was measured using a Qubit dsDNA HS Assay Kit (Invitrogen) and size-distribution of DNA fragments was visualized using an Agilent 2100 Bioanalyzer. Purified DNA was sequenced (single read, 65 bp) on a HiSeq2500 platform (Illumina).