title: 16 S Metagenomic of Cake_2021
design: DNA was purified with Wizard Food DNA kit (Promega), and quantified with a Nanodrop spectrophotometer (Thermo Scientific). The variable V3V4 region of 16S rRNA was amplified using the universal primers 341F and 805R, and barcoded with Nextera indexes, according to manufacturer instructions (Illumina). The amplicons were them quantified with Qubit DNA HS kit (Thermo), and sequenced with NextSeq P1-600 (Ilumina), in pairend 2x300b