title: DupSeq of HD cfdna double captured by bespoke panel
design: 20ng cfDNA library for Duplex Sequencing were prepared with KAPA HyperPrep Kit (Roche, 07962363001) and duplex UMI adapters (IDT). UMI length is a mixed of 3 and 4 bqs.  Personalized panel with 120bp oligos ordered from IDT was used for hybrid capture enrichment. Double capture was perfomred.