title: Double digest RAD-seq of pronghorn
design: Genomic DNA was extracted with a Qiagen DNeasy and double digest RADseq libraries were created following the methods in Parchman et al. (2012), with EcoRI and MseI enzymes and a BluePippin for size selection. Libraries were sequenced with a paired-end 150bp layout on an Illumina HiSeq 4000.