title: GSM7507638: SKC2_siCTL_MCI_Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Treated MEFs were washed with PBS, and RNA was harvested using Quick-RNA prep kit (ZYMO research). RNAseq libraries were constructed with Illumina Truseq RNA Sample Preparation kit v2 according to the manufacturer's instructions and sequenced on a HiSeq 2500 at 1x50 base pairs to a depth of 20-40 million reads. Each RNAseq condition was performed in triplicate, using treated MEF cells with 3 different experiments.