title: RNA-Seq of Flueggea suffruticosa: Root
design: Samples (i.e. root, stem, old leaves >3 months old, young leaves < 3 months old) were isolated from plants, immediately flash-frozen in liquid nitrogen and ground in liquid nitrogen using a pestle and mortar. Roots were washed thoroughly in water prior to freezing. Frozen ground tissue (100 mg) was extracted using a standard CTAB protocol44 including a gDNA removal step (Invitrogen TURBO DNA-free Kit). The quality of the RNA was assessed using an Agilent BioAnalyzer, with all samples achieving RNA Integrity Number >8.4.