title: RNA-seq of scoliotic zebrafish brains: 30dpf control straight spine brains
design: 3 brains were pooled from 30 dpf scoliotic fish and RNA extracted with an RNA extraction kit. RNA sample quality was assessed using an Agilent Bioanalyzer. Library prep and subsequent sequencing was performed by The Centre for Applied Genomics at The Hospital for Sick Children (SickKids) using the NEBNext stranded RNA.