title: Shotgun-seq of Sceloporus grammicus: male adult cloacal swab
design: DNA from cloacal swabs was isolated using the Gentra Puregene Buccal Cell Kit (QIAGEN, Germany). Shotgun genomic libraries were constructed from 300 ng of DNA after sonication with a Covaris ME220 (Covaris, MA) to an average fragment size of 400 bp with the Hyper Library construction kit from Kapa Biosystems (Roche, CA). The pooled shotgun libraries were sequenced on an Illumina NovaSeq 6000 SP lane with 2x150 paired-end configuration.