title: RNAseq of Triticum monococcum
design: Libraries were prepared with a modified protocol of the TruSeq Stranded mRNA Library Prep Kit (Illumina, USA) to obtain library fragments of 250-300 bp (Glmin et al. 2019 Sciences Advances). After verifying and quantifying each indexed cDNA library using a DNA 7500 Chip on a Bioanalyzer 2100, pooled libraries were made of twelve, equally represented, genotypes. Each final pooled library was quantified by qPCR with the KAPA Library Quantification Kit (KAPA Biosystems, USA) and sequenced.