title: GSM8324311: Human PBMCs from AILD AIH patients, GEX Sample 2; Homo sapiens; OTHER
library_construction_protocol: Cells were counted, centrifuged (500g, 5 min, 4°c) then resuspended at the recommended dilution for a 20000 cell loading per sample onto a Next GEM Chip K (PN-1000286) and run on a Chromium Single Cell Controller using the Chromium Single Cell 5′ V2 Next GEM single cell kit (PN-1000263) according to the manufacturer's instructions. Gex and VDJ-TCR libraries were then prepared (PN-1000190, PN-1000252, PN-1000215 also from 10X genomics) The cell hashing protocol described by Stoeckius et al  (Stoeckius et al.,2017)  was used to generate a hashtag oligo (HTO) library, adapted with a 5'v2 immune profiling protocol with TCR. https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf