title: RNA-seq of mouse PGCLC derived from PGC-iPSC rep1
design: Cells were lysed in TRI reagent and total RNA was extracted using a Zymo Direct-zol RNA Miniprep kit. Total RNA was then incubated with RQ1 DNase to remove contaminating genomic DNA and was then re-purified using Zymo RNA Clean & Concentrator-5 kit according to the manufacturers instructions. About 50 ng of total RNA from each sample was used for synthesis and amplification of cDNA. cDNA synthesis was performed by the UTSA Genomics Core using the QuantSeq 3 mRNA-Seq Library Prep Kit FWD from Illumina (LEXOGEN).