title: PromoterHIC_Sciatic nerve crush_KO1
design: Promoter capture Hi-C was performed  from neuronal nuclei isolated from mouse sciatic dorsal root ganglia 3 days after sciatic nerve crush (SNC) or sham conditions from WT (INTACT-AdvillinCre)  and Rad21 KO (INTACT-Scc1flox/flox-AdvillinCre) mice, 14 days after tamoxifen injection. Neuronal nuclei,  expressing nuclear membrane GFP, were immunoisolated via beads bound to anti-GFP and were crosslinked with 2% formaldehyde for 10 min at room temperature.  Nuclei were processed for Hi-C using ARIMA Hi-C Genomics kits (A510008). Briefly, chromatin was digested with multiple enzymes, and purified proximity-ligated DNA was sheared using Bioruptor to obtain an average size of 400 bp. After DNA size selection of 200-600 bp, biotin enrichment, end-repair and adapter ligation, precapture Hi-C libraries were prepared starting from 30 ng of DNA and 14 PCR cycles, using KAPA Library amplification kit (KK2620) or Arima Library preparation module (A303010). After preclearing, hybridization and capture were performed using Arima Mouse Promoter Panel (A302020) starting from 1microg of precapture libraries.  Promoter Hi-C libraries were amplified with 13 cycles PCR.