title: ddradSeq of Lates calcarifer:Offspring 107
design: In brief, 500 ng of DNA from each sample was double-digested with 20 units of EcoRI-HF and NIaIII (New England Biolabs, USA) for 30 min at 37C. Digested DNA fragments were examined by electrophoresis and then ligated with barcoded adaptors of P1 that contained a matching sticky-end and a MID (a short sequence that will uniquely identify the individuals) and P2.