title: Whole Genome Sequencing of Cervical Adenocarcinoma
design: Whole Genome Sequencing of Cervical Adenocarcinoma
library_construction_protocol: 3 tumor samples and paired normal samples of cervical adenocarcinoma were collected at ACTREC-TMC DNA was extracted from tumor tissue and blood normals using qiagen tissue and blood extraction kit the genomic DNA was fragmented by Covaris and fragments of 350bp were selected and A tailing was performed. Adapters were then added to the ends of DNA and amplified using ligation mediated PCR approach which was further subjected to single end separation and cyclization. DNA Nanoballs were produced by rolling circle amplification