title: Rat-D7's RNA-Seq
design: Fecal samples were collected and microbial genomic DNA was extracted using the QIAamp  Fast DNA Stool Mini Kit (Qiagen), according to the manufacturers instructions. The quality of the extracted DNA was measured by an Agilent 4200 TapeStation (Agilent Technologies) kit. A sequencing library was generated by a Nextera XT DNA Sample Prep Kit (Illumina). Whole-genome sequencing was performed on the HiSeq 2513 platform (Illumina). Species composition analysis involves comparing the generated sequences to a database of known microbial species and identifying the microorganisms present in the fecal sample. Functional annotation analysis involves assembling the sequence data into contigs, which are then used to predict gene functions and metabolic pathways of the microbial community.