title: GSM7507663: endoC-BH1 - exp214 - TNFa; Homo sapiens; RNA-Seq
library_construction_protocol: After treatment with the respective cytokines for 24h, total RNA was isolated from EndoC-BH1 cells using the RNeasy Plus Micro Kit (Qiagen, Venlo, Netherlands), according to the recommended protocol. For all the samples, a minimum of 500 ng of high-quality RNA with RNA integrity number (RIN) > 9 (determined using Agilent Bioanalyzer) was used for the library preparation. RNA-sequencing (RNA-seq) was performed on NovaSeq 6000 (Eurofins Genomics Europe Sequencing GmbH, Konstanz, Germany).