title: PacBio HiFi WGS of Hyles lineata: male
design: SMRT bell libraries were prepared at the University of Florida Interdisciplinary Center for Biotechnology Research (ICBR; RRID:SCR_019152) and sequenced on the PacBio SEQUEL IIe according to the recommended protocol (P/N 101-853-100 v. 05, August 2021) with a few modifications. This procedure resulted in ~650 ng of SMRTbell library fragments of ~11.5 kb size. The on-plate loading concentration was 75 pM. The instrument used PacBio Sequencing Kit 2.0 (Cat. # 101-389-001) and Instrument Control SW Version 11.0 (SMRT Link 11.0).