title: RNA-seq of Euplotes euryhalinus
design: The TRIzol plus purification kit (Thermo Fisher Scientific) was used to purify total RNA, following the manufacturers recommendations. On-column DNase treatment was carried out for 45 min at room temperature to obtain DNA-free RNA preparations. After washing, RNA was eluted with 30 l RNase-free water and stored at 80 C before use. cDNA library preparation and sequencing was carried out by BGI using Illumina TruSeq library construction and sequencing at Illumina HiSeq 2000 using 101PE.