title: metagenome sequencing of microbiome: flower
design: The V3 + V4 hypervariable region of the 16S rRNA gene was amplified using primers 341F(5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3) and 805F(5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-21).