title: GSM7100696: Mate-Pair_CKp25-Con; Mus musculus; OTHER
library_construction_protocol: Mate-Pair library preparation was performed using the Nextera® Mate Pair Library Preparation (FC-132-1001). Briefly, formaldehyde-fixed FANS sorted nuclei were reverse crosslinked and genomic DNA was purified. Genomic DNA was tagmented to a size range of 1-5kb using mate pair transposome containing biotinylated adaptors followed by strand displacement to repair single-stranded gaps. Tagmented genomic DNA was circularized and then fragmented using sonication. The junctions were purified using streptavidin beads. Sequencing libraries were prepared using TruSeq DNA LT Sample Prep (15027084) provided with the mate pair kit or NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S) and NEBNext Multiplex Oligos for Illumina (E6440S). Illumina libraries were sequenced in 75bp or 40bp paired-end mode using Nextseq500