title: RNA-seq from MCF7 - KO-Rep3
design: Human cell lines MCF7 (HTB-22) were obtained from ATCC and cultured in high glucose-containing Dulbeccos modified Eagles medium (DMEM, wisent, 319-005-CL) supplemented with 10% FBS and 1% penicillin/streptomycin (Wisent, 450-201-EL). These cells were cultured under puromycin (0.5 g/mL, Wisent, 450-162-XL) selection and all cells were grown at 37 C in a humidified atmosphere with 5% CO2. All hormones and pharmacological compounds were suspended and stored according to the manufacturers guidelines.