title: GSM7432212: animal193_PBS_monogenic_female; Mus musculus; RNA-Seq
library_construction_protocol: RNA from approximately 30 mg of each mouse neocortical tissue was harvested using Direct-zol RNA Microprep   (Zymo Research). 200 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using BGI in house mRNA-Seq Library Prep Kit following manufacturer's protocols (sample QC, mRNA Isolation, mRNA Fragmentation, cDNA Synthesis, End Repair, Add A and Adaptor Ligation, PCR, Library QC, Circularization). Libraries were paired-end sequenced with a read length of 100 bp and an average depth of 20 million reads .