title: delta ggt F. novicida grown in cysteine
design: In brief, 3 g DNA from each sample was sheared to 300 bp on a Covaris LE220 Focused-Ultrasonicator followed by DNA-end repair, terminal C-tailing and of amplification of the transposon-genome junctions by two rounds of PCR. The first round employed the tranposon-specific primer 5-CATCCTGACGGATGGCCTTTTTGCGTTTCTACC-3 and olj376, and second round employed the transposon-specific primer5-AATGATACGGCGACCACCGAGATCTACACTCTTTCGGCATACGAAGACCGGGGACT TATCATCCAACCTG-3 and distal primers as described67 to add unique de-multiplexing barcodes per sample. The libraries were pooled and sequenced using custom sequencing primer 5-GCATACGAAGACCGGGGACTTATCATCCAACC-3 and Read1_SEQ primer as described67 by single-end 150 bp sequencing with a single index read on an Illumina MiSeq at 11 pM density with 15% PhiX spike-in.