title: 16s rRNA sequencing of in-situ microcosms in Hudson Bay
design: In-situ microcosms containing clay beads amended with Alaska North Slope crude oil and 1M NH4H2PO4-NaNO3 (50:50) fertilizer. The microcosms were incubated for one year in Hudson Bay, Nunavut. The DNA from the biofilm on the surface of the beads was recovered with a modified CTAB method and the V4-V5 region of the 16S rRNA gene was amplified with fusion primers. Clean products were pooled and loaded onto an Illumina MiSeq and sequenced using the 500-cycle MiSeq Reagent Kit v2 from Illumina.