title: GSM7324430: C-Inh-11d-Stem-1; Populus tremula x Populus alba; RNA-Seq
library_construction_protocol: The 11-day hormone-treated and control plant tissues were harvested between 10:00 am to 11:00 am (4-5 hours post-dawn). Stem and root samples were frozen in liquid nitrogen and ground into a fine powder using mortar and pestle. Total RNA was extracted from 100mgs of tissue powder using GeneJet Plant RNA purification mini kit (Thermo scientific, USA), and the concentration of RNA was measured using a nanodrop (Thermo scientific, USA). One microgram RNA was shipped to Genewiz (NJ, USA) on dry ice and used for polyA selection, and cDNA library was generated and sequenced using Illumina HiSeq 2x150 bp sequencing. Library construction was performed by GENEWIZ corporation, using polyA selection.