title: Replicate 3 of RNA-seq in wild type
design: 1.5 ug of total RNA from five-day-old wild type (w1118, replicate 3) female guts were purified by TRIzol for library preparation for mRNA-Seq. Sequencing libraries were prepared according to the Illumina TruSeq Stranded mRNA Library prep protocols at Yale Stem Cell Center Genomics Core.  All sequencing data were generated on a NovaSeq S2 or Nextera at the Yale Center for Genome Analysis at Yale West Campus