title: GSM7850348: JSC15-3 DMSO siBRD4 (2); Homo sapiens; RNA-Seq
library_construction_protocol: (1) JSC15-3 cells were treated with 3 µM 5-FU for 5 days, and (2) JSC15-3 cells were transfected with siNCT(negative control) and siBRD4 and treated with or without 3 µM 5-FU for 5 days. RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer's instructions. Sequence libraries were constructed with TruSeq stranded mRNA library preparation kit(Illumina) according to the manufacture's instructions.