title: PacBio HiFi seq of genome-edited E. coli cultures
design: DNA was isolated from E. coli cultures using a QIAamp DNA Mini kit; libraries were prepared with 5-6 kb DNA fragments, the SMRTbell Express Template Prep Kit 2.0, and indexes from the Barcoded Overhang Adapter kit 8A; libraries were sequenced with Polymerase 2.2 for 30h in a Sequel II instrument.