title: GSM7060390: NK_NCT503_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells or tissues with TRIpure Reagent (Bio Teke, China) 1) mRNA enrichment and purification: Oligo dT Selection to enrich the mRNA (For total RNA extracted from human whole blood, globin mRNA are depleted ); 2)RNA fragmentation and cDNA synthesis (second-strand cDNA synthesis with dUTP instead of dTTP); 3)End repair, add A and adaptor ligation; 4)PCR; 5)Circularization and make DNB; 6) Sequencing on DNBSEQ platform