title: Sarcomere Genes
design: Whole exome sequencing library construction and capture: Genomic DNA (300 ng) was randomly fragmented to an average size of 150-200 bp using a Covaris LE220 Sonicator (Covaris, USA). A library was then constructed and captured using the Agilent SureSelect Human All Exon V6 kit (Agilent, USA) following the manufacturer's instructions. Briefly, the purified products were end repaired and A-tailed before ligating with adapters containing unique barcodes. After magnetic bead selection to remove incomplete ligation products and adapter dimers, PCR amplification was carried out using universal primers complementary to the adapter sequences to generate sequencing libraries. The captured exonic sequences were hybridized to the prepared DNA library.