title: GSM8194318: PGCs, HFD, E13.5, WGBS, rep1; Mus musculus; Bisulfite-Seq
library_construction_protocol: The SSEA-1-positive female germ cells obtained through FACS were washed with PBS, and DNA was isolated from the cell pellets using DNeasy Blood & Tissue Kits (QIAGEN). Bisulfite conversion was first performed. The converted DNA was then amplified by nested PCR.