title: GSM7324713: LSD1KOEB_scRNA_2; Mus musculus; RNA-Seq
library_construction_protocol: Day 6 EBs were disscoiated by TrypLE and dissociated cells were resuspended in PBS+ 0.04%BSA. The quantity and quality of the cells were accessed by Acridine Orange and Propidium iodide dye on a Cellometer Auto 2000 (Nexcelom). 16,000 cells were loaded on a 10x Chromium Controller based on 10x Chromium Single Cell 3' Library manual (10x Genomics). After cell partitioning and GEM generation, reverse transcription was performed, and cDNA was pooled and cleaned up by beads. cDNA was further amplified and cleaned up by SPRI beads (Beckman). The quality of cDNA was assessed by High Sensitivity D5000 Tapestation (Agilent Technologies) and quantified by Qubit 2.0 DNA HS assay (ThermoFisher). 3' Gene expression library prep was carried out according to the 10x Chromium Next GEM Single Cell 3' v3.1 manual.