title: Autoclaved lake water 12 degrees Celsius repl. D4
design: For amplification of the V1-V2 hypervariable region of the 16S rRNA gene, we used PCR primers Fw (5- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGMGTTYGATYMTGGCTCAG-3) and Rv (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGCTGCCTCCCGTAGGAGT-3).