title: Mitochondrial DNA sequencing of Chrysemys picta bellii
design: The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followedby 5 and 3 adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligationreactions into a single step that greatly increases the efficiency of the library preparation process.Adapter-ligated fragments are then PCR amplified and gel purified.