title: GSM7596727: Strain_3259_LBS_25˚C_Biol_Rep3; Aliivibrio fischeri; RNA-Seq
library_construction_protocol: Colony material was scrapped from plates and processed using a QIAgen RNeasy PowerBiofilm kit, followed by an additional purification and on-column DNA digestion with the QIAgen Rneasy MinElute kit. For each sample, 500ng of total RNA was used for library construction. Libraries were constructed using the Illumina Ribo-Zero Plus rRNA Depletion with Stranded Total RNA kit, with an addition of V. fischeri specific rRNA depeletion probes. Library preparation was conducted at the University of Wisconsin - Madison Biotechnology Center Gene Expression Center & DNA Sequencing Facility.