title: Transcriptome effects of persistent organic pollutants in the liver of  Atlantic cod (Gadus morhua)
design: Transcriptome effects of persistent organic pollutants in the liver of  Atlantic cod (Gadus morhua)
library_construction_protocol: Juvenile male Atlantic cod from a strain of Norwegian coastal cod were used in this experiment. Farmed cod with mean weight 0.84±0.18 kg was transferred to 20 m3 indoor tanks prior to the start of the exposure experiment. Indoor light followed the natural diurnal rhythm for Bergen (64°N). The fish tanks were supplied with seawater at 8.8-9.5°. The fish were fed once per week (by tube-feeding) feed pellets with vehicle (control), chlorinated compounds (PCBs, DDTs chlordane, lindane and toxaphene), Brominated compounds (PBDE), fluorinated compunds (PFOS) and mixture of  the chlorinated, brominated and fluorinated compounds, as detailed in Bratberg et al., 2013. Tissue sampling was conducted one week after the administration of the final dose. The cod were anaesthetized with metomidate hydrochloride and killed by a blow to the head. Liver samples were rapidly excised, snap frozen in liquid nitrogen and stored at -80°C before further processing. Liver tissue was homogenized with a Precellys 24 homogenizer (Bertin Technologies, Montigny-le-Bretonneux, France). Total RNA was extracted using the BioRobot EZ1 and RNA Tissue Mini Kit (Qiagen, Hilden, Germany) and treated with DNase. The RNA 6000 Nano LabChip kit (Agilent Technologies, Palo Alto, CA, USA) was used to evaluate the RNA integrity of the samples. The RNA integrity number (RIN) was 9.9 ± 0.2 (mean ± st.dev., n=47). Illumina HiSeq 4000 system