title: P1114R_Veh
design: Total RNA was then extracted using the NucleoSpin RNA Mini kit (Macherey-Nagel). Initial RNA integrity numbers (RIN) were measured with an Agilent 5400 Tapestation instrument. For this study, RIN values ranged from 9.1 to 9.9. Libraries were prepared using the TruSeq Stranded Total RNA Gold kit, and sequencing was carried out according to Illumina protocols using a commercial sequencing provider (Novogene, CA). Massively parallel sequencing-by-synthesis with fluorescently labeled, reversibly terminating nucleotides was carried out on a NovaSeq 6000 instrument to provide 150bp paired-end fragments. Resulting FASTQ files were aligned to GENCODE v45(43) using a STAR (v2.7.9) two-pass alignment (44) and RSEM (v1.3.3)(45).