title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in worms exposed to the nematicide cyclobutrifluram.
design: RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in worms exposed to the nematicide cyclobutrifluram.
library_construction_protocol: Control and worms exposed to the nematicide CB (24h) were collected as young adult hermaphrodites 50-h postlarval hatching after hypochlorite treatment (at 20 ì§¸C) (Stiernagle, T. 2006). Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA), and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad CA, USA) according to manufacturer's instructions. cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) at the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/).