title: RNA-seq of Serratia plymuthica A153: 250uM IAA s1
design: RNA sequencing was done at the GENYO Research Center (Granada, Spain). Prior to preparation of the RNA library, ribosomal RNAs were removed from the samples using the RiboZeroTM kit (Epicentre, Gram-Negative-Bacteria#MRZGN126) following the manufacturers instructions. Subsequently, samples were processed with the TruSeq Stranded Total RNA Library Prep Kit (Illumina) following the provided sample preparation guide. The final library (adapter and index included) was validated using the DNA-specific chip Agilent DNA 1000. The final products were fragments between 265 and 300 bp. RNA-seq libraries were sequenced on the Illu mina NextSeq 500 sequencer.