title: GSM6601837: l_w24_2; Mus musculus; RNA-Seq
library_construction_protocol: 2.5*10^5 cells were washed with PBS and resuspend in 200µl TRI Reagent. RNA was extracted from Trizol-samples with a Zymo Direct-zol kit which includes DNAse treatment. RNA quality was assessed with a Fragment Analyzer (Advanced Analytical) and RNA-seq library preparation was performed using Illumina Truseq stranded kit. Sequencing was performed on an Illumina NexSeq 500 machine to produce single-end 76-mers reads. All steps were performed at the Genomics Facility Basel (ETH Zurich).