title: GSM7507915: THP-1-libB T7 Control; Homo sapiens; OTHER
library_construction_protocol: sgRNA assays data analysis was performed as previously described (Julia Joung et al., 2017). In brief, genomic DNA quality was assessed by Uv absorption spectrum, Qubit DNA quantification and Agilent 4150 Tapestation System after extraction from the samples. The genomic DNA library was prepared using the CRISPR2 "Self-balancing library preparation technique" and "Non-bias sgRNA fragment capture technique" developed by Gikegiin. After the completion of PCR library construction, 0.4x and 0.8x AMPure Beads were used to purify product DNA to remove residual primer, template and other sequencing interferences. Agilent 4150 TapeStation High Sensitive D1000 Assay was used to evaluate library quality. The mixed library was sequenced by Illumina HiSeq X and decomposed into each sample data using index sequence. SgRNA reads were obtained from one-way reads and then compared with sgRNA library sequences. Data containing sgRNA are obtained in turn. After illumina sequencing, all the data were visualized using R version 4.0.2 (R development core team). RNA libraries were prepared for sequencing using standard Illumina protocols sgRNA  sequencing