title: IL-33 treatment - Donor 12
design: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. Following end repair, A-tailing, adapter ligation, size selection, amplification and purification, the libraries were checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution. Quantified libraries were pooled and sequenced on Illumina Novaseq 6000. Clusring of the index-coded samples was performed according to manufacturer's instructions.