title: Whole genome sequencing(illumina) of Lactiplantibacillus plantarum KCKM 0106
design: The sequencing libraries were prepared according to the manufacturers instructions of TruSeq NanoDNA High Throughput Library Prep Kit ( Briefly, 100ng of genomic DNA was sheared u singadaptive focuse d acoustic technology Covaris and t he fragmented DNA is end repaired to create5 phosphorylated, blunt ended dsDNA molecules . Following end repair, DNA was size selected withbead based method. These DNA fragments go through the addition of a single A base, and ligationof the TruSeq DNA UD Index ing adapters The products are then purified and enriched with PCR tocreate the final DNA library. The libraries were quantified using qPCR according to the qPC RQuantification Protocol Guide (KAPA Library Quantificat io n kits for Illumina Seque n cing platforms)and qualified using the Agilent Technologies 4 200 TapeStation D1000 screentape (Agilenttechnologies). And then the paired end (2150bp) sequencing was performed by the Macrogenusing the NovaSeq (illumina)