title: RNA-seq of cattle infected with Ostertagia ostertagi: abomasal pyloric mucosa
design: RNA was extracted from abomasal fundic, pyloric mucosa or lymph nodes using TRIzol. Purified RNA was resuspended in nuclease-free water and stored at -80oC until used. RNA quality was assessed using an Agilent Bioanalyzer 2100 system, and all samples with an RIN of 6 or greater were submitted for RNA sequencing by Novogene and Azenta/Genewiz on an Illumina HiSeq 4000 sequencing machine.