title: Glacial and non-glacial sockeye salmon (Oncorhynchus nerka) whole genome sequencing
design: DNA was extracted from tissue samples preserved in 100% ethanol using the DNeasy Blood & Tissue Kit (Qiagen). The extracted DNA samples were purified using the ZYMO DNA Clean and Concentrator Kit. The quality and concentration of the DNA samples were validated using Nanodrop. The sequencing libraties were prepared using 1 ug per sample of DNA with the xGen DNA Library Prep EZ Kit (IDT) and xGen UDI Primers Plate (IDT). The quality of the libraries was validated using the Bioanalyzer High Sensitivity DNA Assay (Agilent) before being sequenced on the NovaSeq S4 200 Lane sequencing platform (Illumina).