title: Nanopore DNA methylation of Otocolobus manul: adult male whole blood
design: DNA was isolated with Quick DNA Miniprep Plus kit (Zymo Research) and seven library preps were performed with the SQK-LSK110 kit (Oxford Nanopore). LIbraries were eluted in 45 microliters and separated into 15 microliter aliquotes, which were each sequenced for 24 hours on a total of seven R9.4.1 minION flow cells (3 aliquots per flow cell with nuclease washes in between). Unmodified bases were called from FAST5s and used to generate the primary assembly. DNA methylation (5mC) at cytosine guanine dinucleotides (CpGs) was then determined by re-basecalling QC-passed FAST5 files with GPU-mode Guppy and a modified base config file (dna_r9.4.1_450bps_modbases_5mc_cg_sup). The primary contig-level assemly was provided to Guppy as a reference.