title: Single-cell RNA-Seq (10x Genomics, 5' R2 only) of 4 human specimens (adult stomach, gastric cancer precursors)
design: Following tissue dissociation, cells were concentrated to 500 - 1,500 live cells/µl. scRNAseq libraries were constructed using the Chromium Next GEM Single Cell 5' version 2 protocol, targeting 10,000 cells with 14 PCR samples for cDNA and library amplification. fastq files were processed with cellranger 3.1.0 and aligned to GRCh38 to generate matrices of unique molecular identifier (UMI) counts per gene and associated cell barcodes. Seurat 4.0.1 was used to construct Seurat objects for each sample and for downstream analyses.