title: Shotgun-seq of Sceloporus grammicus: male adult feces
design: DNA from fecal samples was extracted using a thermo/mechanical disruption method. Shotgun genomic libraries were constructed from 300 ng of DNA after sonication with a Covaris ME220 (Covaris, MA) to an average fragment size of 400 bp with the Hyper Library construction kit from Kapa Biosystems (Roche, CA). The pooled shotgun libraries were sequenced on an Illumina NovaSeq 6000 SP lane with 2x150 paired-end configuration.