title: GSM7787344: Kc167, Input, ChIP in cul4 kd cell, bio rep3; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: 2–3×107 cells were fixed by adding 1% formaldehyde directly to cells in culture medium for 10 min at RT with gentle agitation. 0.125 M glycine was used to quench formaldehyde with gentle agitation for 5 min. Cells were centrifugated at 500 x g for 5 min and washed twice with ice-cold PBS. Chromatin preparation and chromatin immunoprecipitation (ChIP) were performed following earlier described methods (Bag et al., 2019). Libraries were prepared according to the manufacturer's protocol (NEB; Ultra II for DNA Library Prep Kit; E7645).