title: PacBio single molecule real-time sequencing-based first full-length transcriptome of tree tomato
design: Construction of Iso-Seq cDNA library and PacBio Sequencing were performed at Novogene Co., Ltd (Beijing, China). The mRNA was enriched using oligo-dT magnetic beads from 4.0 g total RNA and reverse transcribed into cDNA using the SMARTer PCR cDNA Synthesis Kit (Clontech, now Takara, http://www.takarabio.com). The size-selected cDNA library was constructed according to the BluePippin Size Selection System protocol as described by PacBio (PN 100-092-800-03) and sequenced on the PacBio Sequel platform.