title: Bile-induced biofilm formation in Bacteroides thetaiotaomicron requires magnesium efflux by an RND pump
design: Bile-induced biofilm formation in Bacteroides thetaiotaomicron requires magnesium efflux by an RND pump
library_construction_protocol: Overnight cultures were mixed with RNAprotect (Qiagen) in the anaerobic chamber to prevent RNA degradation and bacteria were lysed using QIAGEN Proteinase K and TE buffer containing lysozyme.  Total RNA was extracted using the Direct Zol kit (Zymo Cat. R2050) according to the manufacturerâ€™s instructions and treated with DNase I from the same kit. RNA concentration, quality, and integrity from 4 independent replicates was checked using RNA6000 Nano chips and the Agilent 2100 Bioanalyzer system ribozero plus was used for ribosomal RNA depletion