title: 16S rRNA seq of pig metagenome: postweaning piglet stool at NA days old
design: Cells in samples were lysed using MP FastPrep-24 5G bead beating system. DNA was extracted using MagMAX-96 Multi-Sample Kit. V3-V4 region of 16S rRNA genes were amplified using custom primers. Clean up of post-PCR products using Taka Bio Nucleo-Mag clean up kit. MiSeq Illumina seuqncing 250 base pair paired end sequencing.