title: 16s rRNA sequencing of in-situ microcosms in Hudson Bay
design: In-situ microcosms containing clay beads amended with Alaska North Slope crude oil. The microcosms were incubated for one year in Hudson Bay, Nunavut. The DNA from the biofilm on the surface of the beads was recovered with a modified CTAB method and the V4-V5 region of the 16S rRNA gene was amplified with fusion primers. Clean products were pooled and loaded onto an Illumina MiSeq and sequenced using the 500-cycle MiSeq Reagent Kit v2 from Illumina.