title: WGBS whole genome DNA methylation landscape of of Homo sapiens: umbilical cord blood
design: DNA isolation, library construction, and sequencing was performed using the Acegen Bisulfite-Seq Library Prep Kit (Acegen, Cat. No. AG0311) following the the protocols prescribed by LC-BIO Bio-tech Ltd (Hangzhou, Zhejiang). Briefly, 1 g of genomic DNA spiked with 1 ng unmethylated Lambda DNA was fragmented by sonication to a mean size of approximately 200-500 bp, end-repaired, 5'-phosphorylated, 3'-dA-tailed and ligated to 5-methylcytosine-modied adapters. Then, the DNA was amplied with 10 cycles of PCR using Illumina 8-bp dual index primers after bisulte treatment. The constructed WGBS libraries were then analyzed by Agilent 2100 Bioanalyzer and finally sequenced using Illumina HiSeq 1502 paired-end sequencing protocol at an average depth of 30X.