title: Dap-Seq  of  Dendrocalamus latiflorus Munro: adult bamboo
design: Genomic DNA libraries were prepared according to (Bartlett et al., 2017). And sonicated to 200 bp fragments in Diagenode Bioruptor Picoruptor. DNA was purified using sodium acetate precipitation method. Samples were then end-repaired and adapter ligation using the VAHTSTM Universal DNAB Library Prep Kit for Illumina V3 (Vazyme) and cleaned using VAHTSTM DNA Clean Beads (Vazyme) according to the manufacturers recommendations. Libraries were quantified with P5 and P7 primers. A quantity of 40 L of purified GFP-PeARF3/6/34 protein (520 g) was diluted in 400 L of 1X PBS containing 25 L of washed GFP-Trap magnetic beads (chromotek) for 1 h at RT on a rotator to bind the protein to the beads. Beads were washed four times in 1X PBS + NP40 (0.005%) and resuspended in 100 L of 1X PBS. One microgram of genomic DNA library was diluted to a final volume of 60 L in 1X PBS and added to the protein bound beads. Protein bound beads and gDNA were rotated for 1 h at RT. Beads were washed four times in 1X PBS + NP40, followed by two washes with 1X PBS. Beads were transferred to a new tube and DNA was recovered by resuspending in 25 l EB and boiling for 10 min at 98 C. Eluted samples were enriched and tagged with dual indexed by performing 20 cycles of PCR in a 50 L reaction. Samples were sequenced on NovaSeq 6000 with 150 bp double-end reads. DNA library negative control. A total of 1032 million reads were obtained for each sample