title: RNA-Seq of adult Toxorhynchites theobald midgut tissue
design: The quality of RNA samples was assessed with the 2100 Bioanalyzer using an RNA 6000 chip (Agilent Technologies). The sample quantification was performed with the Qubit 2.0 Fluorometer (ThermoFisher Scientific). Nucleic acid purity (A260/280 and A260/230) was evaluated using the NanoDrop ND-1000 Spectrophotometer (ThermoFisher Scientific), 400ng of high quality total RNA was used for RNA-seq library preparation using TruSeq RNA Sample Preparation v2 kit (Illumina) according to Illumina protocol. The obtained RNA-seq libraries were analyzed with the 2100 Bioanalyzer (Agilent Technologies) and quantified with Qubit dsDNA BR Assay Kit (ThermoFisher Scientific). Indexed RNA-seq libraries were normalized to 10nM and then pooled in equal volumes, 2 libraries per pool. Each pool was sequenced with one paired end MiSeq (Illumina) run using the 600 cycles MiSeq reagent v3 kit (Illumina).