title: WGS of CKA18: phage gDNA
design: phage genomic DNA was isolated using a Norgen Biotek Phage DNA Isolation Kit (Thorold, Canada). DNA was eluted using nuclease-free water, quantified using a Broad Range QubitTM dsDNA Quantification kit (Thermo Fisher Scientific, Mississauga, Canada), and normalized to 200ng. Genomic DNA was mechanically sheared with a Covaris M220 focused-ultrasonicator (D-Mark Biosciences Inc., Toronto, Canada). Sequencing libraries were prepared using an NxSeq AmpFREE low DNA library kit (Lucigen) and sequencing was performed on a MiSeq device (Illumina) with a  MiSeq rReagent kKit v3 600 cycles (Illumina).