title: GSM7320984: MDA-MB-231_shREN_Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Roche High Pure RNA extraction kit (Roche, 11828665001) according to the manufacturer's instructions. RNA sequencing libraries were prepared using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) in accordance with the manufacturer's instructions. Briefly, 500 ng of total RNA was used for polyA mRNA purification and fragmentation. Purified mRNA underwent first and second strand cDNA synthesis. cDNA was then adenylated, ligated to illumina sequencing adapters, and amplified by PCR (using 8 cycles). Final libraries were evaluated using fluorescent-based assays and were sequenced on an Illumina HiSeq 2500 (v4 chemistry) using 2 x 50 bp cycles.