title: GSM7679620: liver APAP rep1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rats were euthanized and the liver was harvested after compound administration. Briefly, the superior vena cava was clipped using a clamp, and the liver perfusion was performed through an inferior vena cava using a plastic cannula-type puncture needle (SR-OT1832C, 18G 32 mm) (Terumo corporation, Tokyo, Japan) and 5 mM HEPES (345-06681, Fujifilm Wako Pure Chemical Corporation) /5 mM EDTA (6381-92-6, Dojindo Laboratories, Kumamoto, Japan) Hanks' balanced salt solution (17461-05, Nacalai Tesque, Kyoto, Japan). Finally, a part of the liver's largest lobe was subjected to RNA isolation. Total RNA was prepared using ISOGEN II (311-07361, Nippon Gene Co., Tokyo, Japan) and purified using an RNeasy Plus Mini Kit (74136, Qiagen, Limburg, Netherland) with gDNA elimination using an RNase-Free DNase set (79254, Qiagen) according to the manufacturer's protocols. Total RNA was used to prepare RNA-Seq libraries with a NEBNext Ultra ll Directional RNA Library Prep Kit for Illumina.