title: BS-sequencing of CfDNA from colorectal cancer patients
design: Briefly, the procedures were divided into three main steps: (i) multi-cycle linear amplification using single-primer panel, (ii) ligation between amplified ssDNA product and single strand UMI adapter, which contains index sequence, (iii) indexing PCR for amplifying the ligated product with Index Primer. These target enriched libraries were further amplified with P5 and P7 primers and purified for sequencing on the NovaSeq 6000 System (Illumina Inc).