title: Illumina MiSeq PE sequencing of D2_10d_02:U013-P016
design: The genomic DNA (gDNA) from the larval shrimps, filters of the rearing water, and feed were extracted using the DNeasy Power Soil Pro Kit (MoBio Laboratories, USA). A set of barcoded universal primers targeting the hypervariable V4 region (515F/806R) of the prokaryotic 16S rRNA gene was used for the generation of PCR amplicons. 3  l of the template gDNA was used with the forward and reverse primers (final concentration = 0.4  M) at a total volume of 20  l. The amplicons were generated by PCR and the PCR products were purified and pooled to contain an equimolar amount of the PCR product. The library was constructed with the Nextera XT index Kit (Illumina, USA).