title: 16S rRNA amplicon sequencing of A. thaliana rhozomicrobiome exposed to no bacteria control
design: AT Rhizosphere total DNA was extracted from 0.2 g of each sample using the NucleoSpin Soil, DNA isolation kit (Macherey Nagel, MN, USA). PCR amplification of 16S rRNA gene was performed using 515F and 806R. library preparation and sequencing of the rhizosphere samples were performed at the DNA Services Facility (University of Illinois, Chicago, IL, USA), using the Illumina NovaSeq platform..