title: GSM7849662: Hh62; Homo sapiens; RNA-Seq
library_construction_protocol: Sample homogenization was performed using 700 μL of QIAZOL (QIAGEN) either by manual crushing with a pestle or with a gentleMACS Dissociator (Miltenyi Biotec). After homogenization, two supernatants were collected in new 1.5 mL RNase-free microcentrifuge tubes. RNA extraction was carried out using the miRNeasy Micro Kit with a Rnase-Free Dnase Set (QIAGEN). The cDNA libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit and protocol from Illumina (Illumina Inc., San Diego, CA).