title: GSM8243991: Sample_No.53; Homo sapiens; ncRNA-Seq
library_construction_protocol: The total RNA was extracted using the miRNeasy Mini Kit (QIAGEN, Hilden, Germany). Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) from 6 uL RNA. Index codes were added to attribute sequences for each sample.Then, the PCR products were purified using a QIAquick PCR Purification Kit (QIAGEN) on a 6% TBE gel (120 V, 60 min). Furthermore, DNA fragments corresponding to 140–160 bp (length of small RNA plus the 3′ and 5′ adaptors) were recovered, and the cDNA concentration was measured using a Qubit dsDNA HS Assay Kit and a Qubit2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA).