title: Amplicon Sequencing of SARS-CoV-2: Wastewater
design: 30mL WW centrifuged to pellet, resuspended in 500 ul supernatant, extracted with RNEasy Mini Qiagen kit. cDNA synthesized with SuperScript IV First-Strand Synthesis System (ThermoFisher) and PCR amplified using Q5 2x Mastermix (NEB) and ARTIC V4 primers. Library prep using Nextera XT DNA library prep kit (Illumina).