title: Single cell RNA seq of CD45+ immune cells from Mus musculus: adult male/female heart (pooled)
design: The densities of cell suspensions were determined with acridine orange/propidium iodide fluorescence using a LUNA-FX7 cell counter (Logos Biosystems). 14,000-30,000 cells per sample were loaded onto the 10X Genomics Chromium Controller (Chip K). Gene expression and feature barcoding libraries (CD45+ fractions) were prepared using the 10x Genomics Single Cell 5' Reagent Kits v2 (Dual Index) following manufacturer user guide (CG000330 Rev B). The final libraries were diluted to ~10nM for storage. The 10nM library was denatured and further diluted prior to loading on the NovaSeq6000 sequencing platform (Illumina, v1.5 chemistry, 150bp paired end).