title: RNA bind-n-seq on recombinant PfuSmAB1
design: Immobilized RBP candidate was incubated with the indicated randomized input RNA pool. Bound RNA was extracted and cloned for deep sequencing. 3' adapters were subsequently cleaved from cloned libraries to produce a new, specified RNA input pool by run-off transcription for use in a second round of RNA binding with the RBP candidate.