title: GSM7849811: W12E_Prol_145_PRO-seq_1; Homo sapiens; OTHER
library_construction_protocol: Nuclei from W12E cells and Drosophila Schneider 2 (S2) were isolated using the permeabilization buffer. cDNA libraries were constructed by 3' RNA adaptor ligation, 5' phosphorylation, decapping, and 5' RNA adaptor ligation with subsequent reverse transcription and PCR amplification.