title: WGBS of mouse PGCLC derived from SSC-iPSC rep3
design: Cells were lysed in cell lysis buffer with Proteinase K solution and then genomic DNA was extracted from cells using the phenol:chloroform extraction method. Genomic DNA was then purified using Zymo Genomic DNA Clean & Concentrator kit according to the manufacturers instructions. About 50 ng of genomic DNA from each sample was subjected to bisulfite conversion. Preparation of the WGBS libraries was done using the Zymo Pico Methyl-Seq Library Prep Kit according to the manufacturers instructions.