title: GSM7100373: Frozen 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were rinsed once with warm PBS prior to RNA extraction and isolation using the PicoPure RNA isolation Kit (Thermo Fisher Scientific, #KIT0204). 50 µL lysis buffer was added to each scaffold top, placed on a shaker for 5 min at room temperature, and gently scraped with a pipette tip and liquid collected while avoiding bubbles. Samples were incubated at 42˚C for 30 minutes on a heating block in microtubes. Manufacturer's protocols were followed, with the inclusion of a DNAse treatment step (Qiagen, #79254). 21 µL of elution buffer was used in the last step, and a DeNovix spectrophotometer was used to approximate sample RNA concentration and quality. Extraction and isolation were performed in the same sitting. Isolated RNA was kept frozen at -80˚C. Targeted whole transcriptome sequencing was done using the AmpliSeq for Illumina Transcriptome Human Gene Expression Panel (Illumina, Inc) following manufacturer's recommendations.