title: Spatial transcriptomics (10x Genomics Visium FFPE) of 5 human specimens (adult             stomach, 4 gastric cancer precursors and 1 early gastric cancer)
design: Library prep was conducted using the Visium Spatial for FFPE Human                 Transcriptome Gene Expression Kit (version 1) from 10X Genomics, following                 manufacturer's protocol. Probe hybridization, ligation, release, extension, and                 library construction were performed per protocol using 17 cycles for sample index                 PCR. FASTQs were processed using 10X SpaceRanger v1.2.1. Low-quality spots with                 <500 genes detected were removed. Genes detected in =3 spots were excluded. Raw                 counts were imported into Seurat for downstream analysis.