title: GSM7593406: GSC456 treated, STAT5  CUT&Tag; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag was performed with Hyperactive® Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903) according to the provided instructions. ConA beads (concanavalin A-conjugated paramagnetic beads) were added to the cell pellet, and the mixed complexes were incubated overnight at 4°C with 5 µl of STAT5 (Cell Signaling Technology, #94205) or control Rabbit IgG (1 µg) antibodies. The cells were then incubated with a secondary antibody and subsequently linked by pA/G-Tnp. The complexes were sheared and the DNA were extracted. Sequencing libraries were constructed after 15 cycles of PCR amplification. The DNAs were purified using the VAHTS DNA Clean Beads and sequenced on the Novaseq PE150 platform.