title: RNA-Seq of gallua gallus: early embryonic day 12 of pectoral muscle
design: Total RAN was extracted from each sample using Qiagen RNeasy kit, and Ribosomal RNA (rRNA) was removed with the Epicentre Ribo-ZeroTM rRNA Removal Kit (Epicentre, USA) following the manufacturers protocol.Then, the libraries were constructed and paired-end sequencing (2150bp) was performed on the Illumina Hiseq 2505 platform.