title: Single cell RNA-seq analysis of dermis cells from Twist2-Cre; tdTomato mice
design: dorsal skin from Twist2-Cre; tdTomato mice was depilated and digested with elastase (0.2 mg/ml) in DMEM for 20 min at 37C. The tissue was then minced and digested with type IV collagenase (4 mg/ml) in DMEM for 1.5 hrs at 37C. The sample was filtered through a 70 m cell strainer and the remaining cells were washed and pelleted by centrifugation at 500g for 5 min.  Tomato+ cells were sorted using S3e Cell Sorter equipped with a 561 nm wavelength laser.  Single cell libraries was created using the 10X Genomics Chromium Single Cell 3' Library Kit.