title: RNA-seq of BM cells 3rd
design: Total RNA from lineage-depleted mouse bone marrow cells was extracted using RNeasy Kit (QIAGEN). RNA was quantified with Qubit RNA BR Assay Kit (Life technology) and the integrity was evaluated with Agilent Bioanalyzer 2100 (Agilent Technologies). RNA-seq libraries were constructed by MGIEasy RNA Directional Library Prep Set (MGI), according to the manufacturers instruction. The library quality and quantity were verified by Multina (SHIMADZU). Libraries were sequenced with 150 bp paired-end reads on a DNBSEQ-G400 sequencer (MGI Tech, Shenzhen, China).