title: GSM7100353: d7_post_nanoCAR_T_HTO; Homo sapiens; RNA-Seq
library_construction_protocol: Mice were injected with 5x10e5 Raji cells. After seven days, nanoCAR T cells were injected intravenously. Mice were sacrificed seven days post CAR T cell injection. Bone marrow was isolated and processed to obtain a single cell suspension. . Single cell suspension was labelled with antibodies for human CD45, CD19 and hashtag antibodies. The suspension was sorted on a BD ARIA II device based on positivity for human CD45, human CD19 and eGFP. Sorted cells were pooled and resuspended in PBS + 0.04% BSA to obtain on average 1200 cells/µl and loaded on a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads-in-emulsion (GEM) at the VIB Single Cell Core. Library was performed according to the manufacter's instructions (single cell 5' v2 protocol, 10x Genomics).