title: Modelling Multi-Dimensional ClinOmics for Precision Therapy of Children and Adolescent Young Adults with Relapsed and Refractory Cancer
design: Genomic DNA was extracted from patient's normal muscle using Qiagen DNA kits. One hundred nanograms of DNA was sheared to approximately 200 base pairs (bp) by sonication (Covaris, Woburn, MA). Exome enrichment was performed using SureSelect Clinical Research Exome Kits according to the manufacturer's instructions (Agilent, Santa Clara, CA). Paired-end sequencing (2X125 bp) was performed on an Illumina HiSeq2500 instrument.