title: African American adolescent idiopathic scoliosis
design: LIBRARY- Genomic DNA samples were quantified using two platforms- the Qubit Fluorometer 3.0 in combination with the Qubit dsDNA HS Assay Kit (Q32851) and the Varioskan Flash spectral scanning multimode reader from Thermo Scientific, in combination with the Quant- iT Pico Green  double stranded DNA assay kit (P-7589).  Genomic DNA (~600ng) was fragmented on the Covaris LE220 instrument targeting ~375bp inserts.  Automated dual indexed libraries were constructed utilizing the KAPA Hyper PCR-free library prep kit (Roche Diagnostics, Cat #7962371001) on the SciCloneG3 NGS instrument (Perkin Elmer). SEQUENCING- The molarity of each library capture pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit (Roche Diagnostics, cat #7959478001, #7960085001) to produce cluster counts appropriate for the Illumina NovaSeq4000 instrument.  Normalized library pools were sequenced on a NovaSeq4000 Flow Cell (HCS v3.3.52) and a PE 151 cycle sequencing recipe according to manufacturer protocol targeting 6Gb per library.