title: RNA-seq of mus musculus: old stroke microvessels
design: Total RNA was extracted from brain microvessels using TRIzol/chloroform, with concentration, purity, and integrity measured using agarose gel electrophoresis and an Agilent Bioanalyzer RNA 6000 kit. RNA samples with RNA integrity number (RIN) score  5.8 were used to prepare libraries. RNA-seq library construction was generated using the Illumina NovaSeq 600 platform. Total RNA was depleted of rRNA, followed by enzymatic fragmentation, cDNA synthesis, and double-stranded cDNA purification. Each experimental group for RNA-seq consisted of three biological replicates.