title: RNA-Seq of gills tussiue
design: Total RNA from the gills samples was isolated by TRIzol reagent (Qiagen, USA) according to the manufacturers protocol. The RNA was available when the RIN number > 7 in LabChip GX Touch (Perkin Elmer, USA). Simultaneously, the integrity of DNA and RNA was further assessed using agarose gel electrophoresis. The sequencing library was constructed using Truseq Nano DNA HT Sample Prep Kit (Illumina, USA) and Stranded RNA Library Prep Kit (Illumina, USA) for DNA and RNA, respectively. Finally, 153bp pair end library was sequenced using Illumina NovaSeq X (Illumina, USA).