title: GSM6797655: XAF1-ko-ip; Homo sapiens; ChIP-Seq
library_construction_protocol: HT29 cells were crosslinked with 1% formaldehyde and was quenched with 0.125M glycine for 5 min. The cells were washed  and pelleted at 2500 rpm for 5 min. After lysis and sonicate on, the sonicated chromatin was spun down at 12,000 rpm. The soluble chromatin was incubated with 2-5 mg of antibodies overnight. After incubation, pre-washed Protein G Dynabeads (10004d, Invitrogen) were added and incubated for 4 h at in a rotator. Then the magnetic Dynabeads were washed and reverse crosslink. The next day, purify DNA with QIAquick PCR purification kit (Magen, D211102) and elute with 50 mL elution buffer.