title: Whole genome re-sequencing of mutant strain PRM4 resistant to phage vb_PpS_SYP
design: Sequencing libraries were prepared using the standard library building procedure of Illumina's TruSeq DNA PCR-free prep kit reagents. Libraries were quality-checked on an Agilent Bioanalyzer using the Agilent High Sensitivity DNA Kit and qualified libraries had a single peak and no junctional dimers. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Promega QuantiFluor fluorescence quantification system and qualified libraries should have a concentration of 2nM or more. After gradient dilution, the library is denatured to single strand by NaOH for onboard double end sequencing