title: GSM7101385: Batch1_Soleus_JGT02_400 mg/kg_mouse4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA extraction using the TRIzolTM reagent (Thermo Fisher Scientific, #15596018) according to the manufacturer's guide. A total RNA (1 microgr) was processed to prepare a mRNA sequencing library using the MGIEasy RNA Directional Library Prep kit (#1000006386, MGI Tech Co., Ltd., China) according to the manufacturer's instruction. The library was quantified using the QauntiFluor® ssDNA System (E3190, Promega Corporation, WI, USA). The prepared DNA nanoball was sequenced on the MGIseq system (MGI Tech Co., Ltd., China) with 100 bp paired-end reads.