title: GSM7730240: Wild-type mice 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction was performed using RNAiso Plus (Cat# 9180; Takara Bio, Kusatsu, Shiga, Japan), according to the manufacturer's instructions. 500 ng of RNA from each sample was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina and the NEBNext rRNA Depletion Kit v2 (Cat# E7770S and E7400L; New England Biolabs, Ipswich, MA, USA), according to the manufacturer's instructions.