title: K562_PolyASelected
design: CELL CULTURE: K562-MycER cells - from here on named K562 - were grown and maintained in RPMI (Invitrogen Corporation; Cat No. 23400-021) containing 5% FBS in a 5 %C02 incubator at 37C. METABOLIC LABELING: K562 cells were treated with 500uM of 5-Ethynyl Uridine (Jena Bioscience CLK-N002-10) for 60 minutes. RNA EXTRACTION: Total RNA was extracted using Qiazol and mRNA purification was performed with up to 200ug of Total RNA using MACS mRNA Isolation Kit (Miltenyi Biotec 130-075-201). LIBRARY PREPARATION: 100ng of mRNA were used as an input for Nanopore library preparation.