title: RNAseq of LSEC from WT and Bmp9-KO mice in 129/Ola genetic background
design: RNAseq of LSEC from WT and Bmp9-KO mice in 129/Ola genetic background
library_construction_protocol: 25-week-old mice were anesthetized using pentobarbital injection (180mk/kg) and successively perfused through the inferior veina cava with ethylene glycol tetraacetic acid buffer and collagenase II (Merck-Biochrom, Berlin, Germany). Livers were then harvested and cells were isolated using Histodenz (Sigma-Aldrich) gradients. Cell pellets were directly frozen at -80Â°C for bulk RNA sequencing experiments or used for RNA extraction.  All designed samples were treated using poly A+ RNA selection. mRNA extraction was conducted using truseq mRNA Illumina kit.