title: GSM7596766: RNAseq, 11_B3-1_Hepatoblast1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq: RNA was harvested using ZYMO RESEARCH Direct-zol RNA Mini Prep. 1.5 ug of total RNA was used for the construction of sequencing libraries. scRNA-seq: single cells were suspended in PBS containing 0.04% BSA. About 6,000 cells were added to each channel, and the target cell will be recovered was estimated to be about 3,000 cells. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual GEMs. Reverse transcription was performed on a S1000TM Touch Thermal Cycler (Bio Rad) at 53°C for 45 min, followed by 85°Cfor 5 min, and hold at 4°C. The cDNA was generated and then amplified, and quality assessed using an Agilent 4200. RNA-seq: mRNA was purified from total RNA by using poly-T oligo-attached magnetic beads. scRNA-seq: Libraries were constructed using Single Cell 3'Library and Gel Bead Kit V3.1.