title: RNAseq_Sham_KO3
design: RNA-seq was performed from neuronal nuclei isolated from mouse sciatic dorsal root ganglia 3 days after sciatic nerve crush (SNC) or sham  or in nave conditions from WT (INTACT-AdvillinCre)  and Rad21 KO (INTACT-Scc1flox/flox-AdvillinCre) mice, 14 days after tamoxifen injection. Neuronal nuclei, expressing nuclear membrane GFP, were immunoisolated via anti-GFP-bound beads and were directly resuspended in RLT buffer. RNA was extracted with RNeasy Micro kit (Qiagen) and DNase on column digestion following manufacturers guidelines. RNA quality was assessed with Agilent 2100 Bioanalyzer (Agilent). RNA was treated with NEBNext rRNA Depletion Kit and libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, according to the recommended manufacturer protocol. After ligation, the NEBNext Multiplex Oligos for Illumina adapter sequences were ligated to each sample to allow for sequencing multiplexing.