title: NGS panel of Homo Sapiens: pediatric osteosarcoma costal metastasis
design: Tumor profiling was undertaken using the Oncomine Childhood Cancer Research Assay (Thermo Fisher Scientific, Waltham, MA). Libraries were generated using Ion AmpliSeq Library Preparation on the Ion chef System (Thermo Fisher Scientific). Sequencing was performed using 540 chips on the Ion Torrent GeneStudio S5 Prime (Thermo Fisher Scientific).