title: GSM7730163: i704_i511; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen RNA extraction kit Library preparation was performed based on the protocol from Picelli et al. (doi: 10.1038/nprot.2014.006) modified as follows: 1) using a set input of 500pg for each sample in 2uL volume 2) starting protocol at reverse transcription step 3) number of preamplification cycles changed from 21 to 16 4) cDNA input amount for library prep step with Illumina Nextera XT kit was 0.2ng 5) library pooling and clean up step modified by quantifying each sample individually and making an equimolar pool for sequencing.