title: SoJIC_oldleaf_rep3
design: RNA was extracted via RNeasy Plant Mini kit (Qiagen). Along with RNA extraction, on-column DNase digestion was performed using RQ1 RNase-Free DNase (Promega). RNA sequencing and assembly was carried out by the Earlham Institute. The RNA-seq library was prepared using NEBNext Ultra II Directional RNA-seq library preparation kit and was subsequently sequenced on two lanes of NovaSeq 6000 SP flow cell (150 pair-end reads).