title: GSM7989409: D456, NT, 1; Homo sapiens; RNA-Seq
library_construction_protocol: Norgen Total RNA kit plus DNase treatment according to the manufacturer's protocol We used 800 ng of total RNA as input to the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB cat# E7490S). PolyA depleted RNA was used as input to the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB cat# E7530S). Libraries were barcoded using the NEBNext Multiplex Oligos for Illumina (NEB cat# E7335S).