title: WGS of Dirofilaria immitis: single microfilaria from Queensland, Australia
design: Single microfilariae were isolated from dog blood and whole genome amplification was performed using the GenomePlex Single Cell Whole Genome Amplification Kit (Sigma-Aldrich, Australia). DNA was purified with the QIAquick PCR Purification Kit (QIAGEN, Australia). DNA samples were sent to Novogene for library construction and Illumina whole genome sequencing.