title: single-cell RNAseq of mus musculus: adult brain
design: The cDNA and library were generated using the Chromium Next GEM Single Cell 3 Reagent Kits v3.1 (10X Genomics, PN-1000286) and single Index Kit T Set A (10X Genomics, PN-1000213) according to the manufacturers manual. For cell hashing library, it was constructed according to Biolegends published protocol on HTO library preparation guideline with single index adapters. Quality control for constructed library was performed by Agilent Bioanalyzer High Sensitivity DNA kit (Agilent Technologies, 5067-4626) and Qubit DNA HS assay kit for qualitative and quantitative analysis, respectively. The multiplexed libraries were pooled and sequenced on Illumina HiSeq 4000 sequencer with 2  50 paired-end kits using the following read length: 28 bp Read1 for cell barcode and UMI, and 90 bp Read2 for transcript.