title: 16S rRNA of soil: restored peatland soil with ~5 cm water level
design: Soil DNA was extracted from each soil sample using a Fast DNATM SPIN Kit (MP Biomedicals, CA, USA). The concentration and purity of DNA extractives were examined and sent to Shanghai Majorbio Technology Co., Ltd., China, for high-throughput sequencing. The primer pairs 338F (5-ACTCCTACGGGAGGCAGCA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) were used to amplify the bacterial 16S rRNA gene. The primer pairs 524F10extF (TGYCAGCCGCCGCGGTAA) and Arch958RmodR (YCCGGCGTTGAVTCCAATT) were used to amplify the archaeal 16S rRNA gene. Paired-end sequencing was conducted the Illumina MiSeq PE300 platform.