title: GSM6797178: posterior epiblast, E8.5, RAL, rep1; Mus musculus; ATAC-seq
library_construction_protocol: Tissue samples were incubated with accutase for 30 minutes to dissociate into single cell suspension. After lysis and transposition reaction, genomic DNA was extracted with MinElute purification kit (Qiagen) ATAC-seq libraries were prepared using NEBNext High-Fidelity 2× PCR Master Mix with custom-synthesized PCR primers (Buenrostro et al, 2013). Libraries were amplified using 8 cycles in the thermocycler. The amplified libraries were size selected using SPRIselect beads from Beckman Coulter.