title: Amplicon seq rhizosphere Circoval
design: For sequencing analysis, samples were sent to Novogene, Company Limited (Cambridge, UK). The NGS was performed in duplicate using the targeted the V3 and V4 regions of the bacterial 16S rRNA gene using the forward and reverse primers 341F (CCTAYGGGRBGCASCAG) and 806R (GGACTACNNGGGTATCTAAT) (Klindworth et al., 2013). While for the ITS1 regions of the fungal communities, primers were ITS1f-F (CTTGGTCATTTAGAGGAAGTAA) and ITS1-1F-R (GCTGCGTTCTTCATCGATGC) (Gardes and Brunes, 1993). The generated DNA libraries were sequenced with MiSeq Reagent Kit Nano in the lllumina MiSeq platform, using 250bp paired-end sequencing reads.