title: GSM7989222: THP-1 cells, Control R2, RNA-seq biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: For freshly cultured cells, total RNA isolation and DNaseI treatment was performed using Direct-zol(TM) RNA MiniPrep kit (Zymo Research, R2053) as per manufacturer's protocol. Library preparation was conducted using the NEBNext UltraII RNA library kit (NEB#E7770S/L). Sequencing was conducted on an Illumina NextSeq 2000.