title: GSM8421622: DP_EED_R3; Mus musculus; OTHER
library_construction_protocol: At endpoint, tumors were collected, minced into small pieces using scalpels and digested with 2 mg/ml collagenase IV, 2mg/ml hyaluronidase and 2 mg/ml BSA in DMEM/F12 at 37C for 60 minutes. Afterwards, cells were stained with LIVE/DEAD Aqua, CD45 and Epcam antibodies and sorted for live cells, CD45-negative, Epcam-positive cells. Cut&Run libraries were prepared using IDT xGen DNA library prep reagents on a Beckman Coulter Biomek i7 liquid handling platform from approximately 1ng of DNA with 14 cycles of PCR amplification according to manufacturer's protocol. Finished sequencing libraries were quantified by Qubit fluorometer and Agilent TapeStation 2200. Library pooling and indexing was evaluated with shallow sequencing on an Illumina NovaSeq X Plus. Subsequently, libraries were sequenced on an Illumina NovaSeq X Plus targeting 20 million 150bp read pairs by the Molecular Biology Core facilities at Dana-Farber Cancer Institute.