title: 3D7A parent line
design: Sample library construction was performed using the Kapa Hyper Kit (Roche, Wilmington, MA).  Firstly, DNA samples are fragmented to an average size range of 300 bp using a Covaris E220 shearing instrument. Adapter-ligated libraries are generated and enriched by polymerase chain reaction (PCR) following the manufacturers instructions. The normalized DNA libraries are pooled and sequenced on Illumina NovaSeq 6000 sequencer (Illumina, San Diego, CA) with pair-end 150 cycles. The raw sequencing reads in BCL format was processed through bcl2fastq 2.19 (Illumina) for FASTQ conversion and demultiplexing for downstream data analysis.