title: Amplicon Sequencing of SARS-CoV-2: Wastewater
design: Torpedo Sampler left in flow for 24hr, gauze stomached in PBS and viral particles captured using ceres microbiome A magnetic beads, extracted with QIAamp Viral RNA extraction Mini kit (Qiagen). cDNA synthesized with SuperScript IV First-Strand Synthesis System (ThermoFisher) and PCR amplified using Q5 2x Mastermix (NEB) and ARTIC V4 primers. Library prep using Nextera XT DNA library prep kit (Illumina).