title: GSM7507239: T2_2; Danio rerio; RNA-Seq
library_construction_protocol: Total RNAs were extracted from embryos at three key organogenesis stages in zebrafish using TRIzol reagent(Invitrogen, cat. NO 15596026) following the methods by Chomczynski et al (DOI:10.1006/abio.1987.9999). 2 μg total RNAs were used for stranded RNA sequencing library preparation using Ribo-off rRNA Depletion Kit  (Catalog NO. MRZG12324, Illumina) and KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.