title: RNA bind-n-seq on overexpressed Pumillo1 (mmu)
design: Immobilized RBP candidate was incubated with the indicated randomized input RNA pool. Bound RNA was extracted and cloned for deep sequencing. 3' adapters were subsequently cleaved from cloned libraries to produce a new, specified RNA input pool by run-off transcription for use in a second round of RNA binding with the RBP candidate.