title: GSM8421750: Sample_6_KI_anti_IFNAR1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from whole spleen with Trizol according to manufacture's protocol (Thermo Fisher) followed by DNAse treatment. cDNA libraries libraries were prepared at the Next Generation Sequencing Core Facility of the Medical Faculty/West German Genome Center Bonn at the University of Bonn using the QuantSeq 3´-mRNA Library protocol from Lexogen. Sequencing was performed on an Illumina NovaSeq 6000 platform to generate 1x100bp reads resulting in an average depth of 10 million Raw Reads per sample.