title: GSM6704372: (A) Steady state neutrophils, before infection, rep 4; Danio rerio; RNA-Seq
library_construction_protocol: Larvae were homogenised in 0.25% trypsin with mechanical agitation prior to FACS-isolation of 1000 neutrophils per sample. cDNA was synthesised directly from cell lysates using the SMART-Seq v4 Ultra Low Input RNA kit for sequencing (Takara Bio) Libraries were made using the Nextera XT DNA Library Preparation Kit (Illumina).