title: GSM7143082: shTHBD22; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using an RNAeasy kit (Qiagen) and dissolved in RNase-free water. RNA samples were sequenced by Novogene Corporation. In brief, RNA degradation and contamination were monitored on 1% agarose gels. RNA purity was checked using a NanoPhotometer Spectrophotometer (IMPLEN). RNA integrity and quantity were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Sequencing libraries were generated using the NEB Next Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer's recommendations and index codes were added to attribute sequences to each sample. Clustering of index-coded samples was performed on an Illumina NovaSeq sequencer