title: 18S metabarcoding of Arctic Ocean microbial eukaryotes
design: Total RNA was extracted with the RNeasy Mini Kit (QIAGEN) and reverse transcribed to cDNA using a SuperScript kit (Invitrogen). PCR-amplification of the V4 region of the 18S rRNA was carried out. Amplicon sequencing was run on Illumina MiSeq using 300+300 bp paired-end V3 chemistry at Dalhousie Universitys Integrated Microbiome Resource (Halifax, Nova Scotia, Canada).