title: whole exome sequencing of homo sapiens: female peripheral blood
design: A DNA library was prepared by randomly interrupting the 180-280bp segment of genomic DNA by Covaris fragmenter. After terminal repair and A-tail addition, a splice was attached to both ends of the fragment. After library pooling with specific index, the library is hybridized with the biotin-labeled probe in liquid phase, and magnetic beads with streptomycin are used to capture the exon on the gene. After PCR amplification, the library can be sequenced.