title: PE Miseq reads from gDNA from overnight cultures
design: Genomic DNA (gDNA) was extracted from the culture used to inoculate calves with the DNeasy blood and tissue Genomic-tip kit (Qiagen, Hilden, Germany). gDNA quality was assessed by Nanodrop spectrophotometry (Thermo Fisher Scientific, Waltham, MA) and a Qubit fluorimeter DNA broad range kit (Thermo Fisher Scientific, Waltham, MA). Nanopore libraries were prepared with rapid barcoding kit (SQK-RBK004) according to the manufacturers instructions (Oxford Nanopore, Oxford, U.K.). The Nextera Flex kit (Illumina, San Diego, CA) was used to prepare the genomic library for MiSeq sequencing.