title: Shotgun metagenomics sequencing of in-situ microcosms in Hudson Bay
design: In-situ microcosms containing clay beads amended with Alaska North Slope crude oil. The microcosms were incubated for one year in Hudson Bay, Nunavut. The DNA from the biofilm on the surface of the beads was recovered with a modified CTAB method and and libraries were prepared using the Nextera XT DNA Library Preparation Kit from Illumina. Products were pooled and sequenced on an Illumina NovaSeq on a 2 x 150 bp configuration.