title: RNA-seq of Aporrectodea caliginosa
design: RNAseq was performed at the GeT-PlaGe core facility, INRA Toulouse. RNA-seq libraries have been prepared according to Illuminas protocols using the Illumina (San Diego, CA, USA) TruSeq Stranded mRNA sample prep kit to analyze mRNA. Briefly, mRNA was selected using poly-T beads from 2 g of total RNA. Then, RNA was fragmented to generate double stranded cDNA and adaptators were ligated to be sequenced. 11 PCR cycles were applied to amplify libraries. Library quality was assessed using a Fragment Analyser and libraries were quantified by qPCR using the Kapa Library Quantification Kit. RNA-seq analyses have been performed on an Illumina HiSeq3000 using a paired-end read length of 2x150 pb. For each sequencing lane of a flowcell, thirty libraries were pooled in equal proportions.