GSM7597599: Mouse HGG-MYCN_7; Mus musculus; RNA-Seq
Tumors of four hGFAP-cre::TP53Fl/Fl::lsl-MYCNFl/wt mice were isolated and minced. To prepare single-cell suspensions, samples were digested with papain and DNAse, and passed through a 40 µm cell strainer. Red blood cells were depleted using red-blood-cell  lysis buffer and non-vital cells were removed using 7-Aminoactinomycin D staining. 10,000 vital cells were used as input for scRNA-seq. Dual-index libraries were generated with Chromium Single Cell 3' v3.1 technology (10x Genomics) and sequenced using a NextSeq 2000 sequencing instrument (high-throughput kit, 100 cycles).