GSM7911836: Control3, DIV70; Homo sapiens; RNA-Seq
For RNA-seq experiments, patient iPSC-derived neurons were plated and aged to ~10 weeks as previously described. Total RNA was extracted using TRIzol and chloroform, followed by isopropanol precipitation. RNA pellets were washed in 75%+ ethanol and resuspended in nuclease free H2O. Contaminating gDNA was removed by DNase I digestion (Fisher Scientific) and cleaned up using an RNA clean & concentrator kit (Zymo). Total RNA was quantified by NanoDrop UV/Vis spectrophotometry, analyzed for integrity by Bioanalyzer, and 1.5-2 µg total RNA was sent to Genewiz (Azenta) for rRNA-depletion library preparation and paired-end (PE) sequencing, where between 20-25m paired-end reads were achieved per sample. Conducted by Azenta.