GSM7754753: OF045; Homo sapiens; RNA-Seq
Total RNA was extracted from frozen brain tissue samples using an automated Qiagen platform (QiaSymphony). RIN values were measured on an Agilent Bioanalyzer. All samples had RIN values > 7. The samples were processed using an Illumina® Stranded mRNA Prep kit and were sequenced on an Illumina NOVASEQ 6000 sequencer to produce stranded 100-base pair paired end libraries at 20 million read depth per sample. For RNAseq of microglial cultures, the samples were processed using an Illumina TruSeq Stranded total RNA kit and sequenced on an Illumina NextSeq sequencer at a read depth of 10 million reads per sample.