GSM6645061: submarginal zone between the folds and pallial line; Perna viridis; RNA-Seq
Tissues (50-100 mg) were frozen in liquid nitrogen and ground them into fine power with a pestle and mortar and the total RNAs were extracted using Total RNA Extraction Reagent (TAKARA Japan) according to the user instruction. Genome contamination was removed by DNase I.1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using Illumina TruseqTM RNA sample prep Kit following manufacturer's protocols. The RNA library was prepared following TruSeqTM RNA sample preparation Kit from Illumina (San Diego, CA) using 1μg of total RNA