Whole genome sequencing of Cryptosporidium equi
The purified oocysts were subjected to eight freeze-thaw cycles and overnight digestion with proteinase K. Genomic DNA was extracted from the oocysts by using the QIAamp DNA Mini Kit and amplified by REPLI-g Midi Kit. For whole genome sequencing, 250-bp paired-end reads were generated from the DNA by using Illumina Novaseq 6000 analysis of an Illumina TruSeq (v3) library.