GSM7818036: daf2_1; Caenorhabditis elegans; RNA-Seq
Total RNAs were extracted from the day 1 adult worms using RNA Isoplus (Takara) extraction methods. For each condition [wild-type, daf-2(e1370), hel-1(gk148684), or hel-1(gk148684); daf-2(e1370)], two independent biological repeats were used and processed as duplicates until final analysis. mRNAs were extracted and converted to cDNAs by reverse transcription. The cDNAs were sequenced using a HiSEq 2500 platform (Chunlab Inc.).