GSM7790047: mP2; Mus musculus; Bisulfite-Seq
Media was removed from Matrigel domes, and 4-6 wells of organoids were harvested in 1 mL of mouse splitting medium [DMEM F12 Advanced (Invitrogen), 10 mM HEPES pH 7.2-7.5 (Invitrogen), 1X GlutaMax (Invitrogen), 1X Penicillin/Streptomycin (Invitrogen)]. DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit per manufacturer's instructions. Swift BioSciences Accel-NGS Methyl-Seq DNA Library Kit Whole genome bisulfite sequencing (WGBS)