GSM7851772: P4092; Homo sapiens; RNA-Seq
Frozen adipose tissue was disrupted using ceramic beads and grinder system (MP Biomedicals LLC FastPrep-24 5G and Lysing Matrix D tubes with 1.4mm Ceramic beads for the discovery cohort) in Qiazol Lysis Reagent. RNA was purified using the Qiagen RNEasy Plus Mini kit. An on-column DNAse digestion was performed for the discovery and VA cohorts. RNA was frozen at -80 degrees Celsius until sequencing. The NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) was used. ssRNA-seq