RNAseq data for phs003642
Total RNA was isolated from each hNPC line. For each experimental week, hNPCs were lifted with Accutase (Thermo Fisher Scientific A1110501), lysed, and stored in TriZol (Thermo 15-596-026) at -80C for subsequent purification of total RNA using RNeasy Mini Kits and on-column DNase digestion (Qiagen 74106, 79256). We quantified RNA isolates using fluorescence-based Quant-iT RNA assay kits (Thermo Q33140). Mean RNA integrity number (RIN) across all samples was 9.86 (SD = 0.39). Library preparation was performed using a stranded total RNA kit (KAPA KR0934) after samples were depleted of ribosomal RNA (KAPA KR1151). RNA-seq libraries were sequenced at the NYGC to an average sequencing depth of 55M (SD = 13M) paired-end reads (2x100bp) per sample on the Illumina Novaseq platform.