SC 15 ft
RNA was extracted using a ZymoBiomics DNA/RNA MiniPrep Kit, cDNA was sythesized with the Thermo Superscript IV first strand synthesis kit. and cDNA was amplified with primers adapted from Parada et al. (2015) as described in Stamps et al. (2016); barcoded, purified and pooled amplicons sequenced on an Illumina MiSeq at the Genomics and Microarray Core at the University of Colorado Anschutz Medical School ; v2 paired-end 2 x 250 bp chemistry.