GSM6705361: hepatocytes_96hpf_wt_expt2_rep3; Danio rerio; RNA-Seq
Hepatocytes were sorted into 75 uL Buffer RLT containing b-mercaptoethanol. Total RNA was extracted using the Qiagen RNA Micro Kit with on-column DNase treatment. cDNA synthesis and amplification were performed according to the Smart-seq2 protocol (https://doi.org/10.1038/nprot.2014.006). Library prep was performed with the Nextera XT DNA Library Preparation Kit (Illumina).