GSM7789949: mesc_eto_treat_rep1; Mus musculus; RNA-Seq
Isolated single cell was lysed wih gentle lysis buffer (containing 0.45% NP-40), and the supernant was collected for the construction of sequencing library. Collected supernant was amplified with standard Smart-seq3 protocol, then perform library preparation with homemade Tn5 transposase. Smart-seq3 & bulk RNA-seq