GSM6743940: Blastoid_4CL, scRNAseq; Macaca fascicularis; RNA-Seq
Monkey blastoids were picked up manually by mouth pipette. Then blastoids were washed with PBS containing 0.05% BSA and dissociated with trypsin-EDTA (0.25%) (Gibco) at 37 °C in 5% CO2 for 10 min. The dissociation was terminated by PBS with 15% FBS and centrifuged the sample at 300g for 5 min. Cell pellets were resuspended in PBS containing 0.05% BSA, filtered through a 40-μm cell strainer and counted. Single-cell suspensions were loaded into a 10X Genomics Chromium Chip following the manufacturer's instructions 48 (10X Genomics, Chromium Next GEM Single Cell 3ʹ GEM, Library and Gel Bead Kit v.3.1).