GSM7990476: GC4; Homo sapiens; OTHER
Spatial transcriptomics were performed using Visium assay (10X Genomics) as manufacturer's recommendation. Surgical sections were prepared and stored in OCT. 10um tissue sections were prepared for HE stain to determine the regions of interests (6.5umX6.5um) and further subject to permeabilization to capture RNA molecules onto barcoded spots. Sequencing library construction as manufacturer's protocol.