RNA-Seq of HL-60/S4 cells with lentivirus exprssing gfp siRNA
Sample was thawed by the addition of the RLT lysis buffer from the Qiagen RNeasy Mini Kit and RNA purified according to the manufacturers protocol.  Care was taken to maintain RNAase-free conditions with RNAase Zap.  RNA was eluted with molecular biology grade water, frozen in LN2 and shipped on dry ice to the Marshall University Genomics Core Facility.  QC determinations (all 12 samples had a RIN score of 10),preparation of the libraries (Illumina mRNA library preparation Kit) and sequencing was carried out at the Core Facility.