WGS of Campylobacter jejuni: broiler chicken caeca
Both C. jejuni strains were grown overnight on OMHA + bloood plates under microaerobic conditions and genomic DNA was extracted using Epicentre Metagenomic DNA Isolation kits for Water (Illumina) according to the manufacturer's instruction. Briefly, quantification of DNA was performed by DeNovix QFX Fluorometer. Sample libraries were prepared using a MiSeq Nextera XT DNA library preparation kit (Illumina). Whole genome sequencing was performed by 250 bp paired end read sequencing on the Illumina MiSeq sequencer using a MiSeq Reagent Kit V2 and 500 cycles on the Illumina MiSeq platform.