DNA sequencing data of PPP1R12C and KCNH2 genomic regions in cell lines modified at these loci using STRAIGHT-IN.
DNA sequencing data of PPP1R12C and KCNH2 genomic regions in cell lines modified at these loci using STRAIGHT-IN.
Approximately 1x10^6 hiPSCs (cell line described here: https://hpscreg.eu/cell-line/LUMCi028-A)  were rinsed once with PBS without Mg2+ and Ca2+ (PBS-/-) and dissociated to single cells with 1x TrypLE Select (ThermoFisher). The dissociation reagent was neutralized with culture medium (DMEM/F12, BSA 0.5%) and the pellet of cells was resuspended in PBS-/- and kept at -20°C until the genomic DNA extraction protocol was performed. Genomic DNA was extracted from hiPSCs using the High Pure PCR Template Preparation Kit (Roche) and treated with RNAse A (10 mg/ml, Invitrogen) according to the manufacturers' instructions. The nucleic acid library was constructed using the NEBNext® Ultra II FS DNA and Ligation kits to fragment, A-tail and ligate sequencing adapters to the genomic DNA. The size of the resulting product was  approximately 500-700 bp.