GSM6644911: 50F2_Day_18; Mus musculus; RNA-Seq
Cells were cultured at optimal cell number to maintain ≥1000-fold coverage of the CRISPR sgRNA library. At day 0 and 18 (~14 doublings), DNA isolated from harvested viable cells was subjected to targeted deep sequencing of sgRNAs Libraries were prepared using NextSeq HIGH 150 v2.5 kit  according to the manufacturer's instructions. NextSeq HIGH 150 v2.5 kit