GSM8246787: HDF-3 shAR-1_IgG; Homo sapiens; OTHER
Three different HDF strains were infected either with lentiviruses expressing shAR-1 or shCtrl, selected with puromycin and harvested for Cut & Run experiments 7th day post infection. Briefly, 500,000 cells were incubated with Protein A-MNase fusion protein, digested with Micrococcal Nuclease, and nuclei were washed and incubated with Protein A-G-MNase beads for elution. Cut & Run assays were performed using the CUTANA™ ChIC / CUT&RUN Kit ( EpiCypher; Cat. No. # SKU: 14-1048) to investigate chromatin interactions of pSer301 and total Lamin A/C in parallel to H3K27Ac in HDF cells with and without AR silencing. DNA purification and library preparation were performed, followed by Illumina sequencing