GSM7029320: RNA-Seq2 KO Rep2; Mus musculus; RNA-Seq
Total RNA was extracted from cells using RNeasy Mini Kit (74106, QIAGEN, Hilden, Germany) RNA library preparation and sequencing were performed by the DNA sequencing core at the University of Michigan. Briefly, the RNA library was prepared with NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA) and sequenced on a NovaSeq 6000 platform (Illumina) to generate pair-end 51 bp reads according to the manufacturer's recommendation. In total, ~360 million reads were generated with an average of 30 million paired-reads per sample.