GSM7949348: PSC_young; Mus musculus; RNA-Seq
Freshly collected Bone and periosteum were digested with 1 ml pre-warmed digestion solution (200 U/ml DNase I (Sigma, 11284932001), 2 mg/ml collagenase I (Worthington, LS004197) and 2 mg/ml collagenase IV (Worthington, LS004189) in HBSS(ThermoFisher, 14025134)) and incubated in a shaking water bath at 37°C at a speed of 220 rpm for 30 minutes. After digestion, single-cell suspension was filtered through a 40-µm nylon mesh and washed twice in ice-cold HBSS without Ca2+ and Mg2+ (ThermoFisher, 14170138).Then cells were sorted by Aria sorp(BD) A single cell library was generated using Chromium Single Cell 3' Library Kit v2 (10× Genomics). Library sequencing was performed on an Illumina Hiseq PE150 or NovaSeq PE150 platform.