GSM8247004: blood, AT-34; Homo sapiens; miRNA-Seq
small RNAs was then extracted from PBMCs using the miRNeasy Kit (Qiagen, Germantown, MD, USA) according to the manufacturer′s instructions. 250 ng of total RNA was used for the construction of sequqncing libraries. Libraries were prepared from 250 ng of total RNA using NEGEDIA smallRNAseq sequencing service (Next Generation Diagnostics srl) which included, library preparation, quality assessment and sequencing on a NovaSeq 6000 sequencing system using a single-end, 50 cycle strategy (Illumina Inc.). NEGEDIA smallRNAseq