GSM8421921: D18WT3; Homo sapiens; RNA-Seq
Total RNA was extracted with TRIzol (Invitrogen) and treated with DNase I (NEB) according to the manufacturer's instructions. The rRNA-depleted RNA-seq libraries were prepared according to previously described protocols.33 Briefly, the Ribo-Zero rRNA Removal Kit was used to deplete rRNAs, and the NEBNext UltraTM RNA Library Prep Kit was used to prepare libraries.