GSM7818491: MCF_9; Homo sapiens; RNA-Seq
After treatment, cells were washed with 37 °C PBS and mRNA was isolated RNA was extracted in phenol: chloroform: isoamyl alcohol followed by ethanol precipitation. DNA was removed by DNase I treatment. mRNA library was prepared by polyA selection with ≥500 ng RNA using the Universal Plus mRNA-Seq Library Preparation Kit (NuGEN) following manufacturer's protocol.