GSM7912366: Bulk VEGFaR84_3; Mus musculus; RNA-Seq
The neural dissociation kit (P) was used to dissociate the spinal cords into a single cell suspension. Following myelin removal using Miltenyi Biotec Myelin Removal Beads II, the samples were stained with CD31-APC and PI, and live endothelial cells were FACS sorted for single cell RNA sequencing Bulk RNA-seq: mRNA was enriched from total RNA samples after sorting cells (see extract protocol) using poly-A pull down and cDNA libraries were prepared using Illumina Truseq chemistry, followed by sequencing using Illumina NovaSeq 6000. Single cell RNA-seq: Single cells were processed on the 10x Genomics Chromium platform (v3 chemistry), and cDNA libraries were sequenced on the Illumina NovaSeq6000