GSM8149656: Liver, water, db/db, rep2; Mus musculus; RNA-Seq
Total RNA was isolated from tissue samples using the TRIzolTM Reagent. 1 mL of the reagent and a stainless-steel bead were added to the tissue samples, and tissues were fully homogenized by the TissueLyser LT homogenizer. 1000 ng of total RNA was processed to mRNA enrichment using NEBNext Poly(A) mRNA magnetic isolation module (New England BioLabs, MA, USA) and followed by fragmentation, cDNA synthesis, and library preparation with unique dual index using NEBNext Ultra II RNA library prep kit (New England BioLabs) according to the manufacturer's manual.