GSM6703889: miR-142 KO CML LSK, scRNAseq; Mus musculus; RNA-Seq
~8000 cells were captured per sample on a 10x Genomics Chromium using a Next GEM Single Cell 3' GEM kit V3.1 (10xGenomics, Cat. PN- 1000121). All protocols were performed following the manufacture's instruction. Final sequencing libraries were analyzed on a High Sensitivity DNA Chip (Agilent, Cat 5067-4626) and quantified with a Qubit high Sensitivity DNA assay Kit (Thermo, Cat. Q32854). The sequencing was performed on Illumina NovaSeq6000 with S4 Reagent v1.5 kit (Illumina, Cat 20028313) at Tgen with the sequencing length of 28+10+10+101. Real-time analysis (RTA) 3.4.4 software was used to process the image analysis. Single cell 3' gene expression library was prepared and final sequencing libraries were analyzed on a High Sensitivity DNA Chip (Agilent, Cat 5067-4626).