GSM7062576: T51_Exp2_T51_CONT_S53_L004; Homo sapiens; RNA-Seq
RNA extractions from cultured cells were performed by the RNeasy Mini kit (Qi-agen, Hilden, Germany). Library preparation and RNA sequencing were conducted at National Jewish Health (Seibold Laboratory). RNA-seq libraries were generated on 81 ALI samples (3 replicates for 3 donors for 9 treatments). KAPA mRNA Hyper Prep (Roche) whole-transcriptome libraries were constructed on the Beckman Coulter FXP automation system with 500 ng RNA input per sample, and Illumina Dual Index Adapters (IDT Technologies) were used to barcode samples using 10 cycles of amplification. Barcoded libraries were pooled and sequenced on an Illumina NovaSeq 6000 system using 150bp paired end reads.