ACH-2 with fentanyl replicate 1
ACH-2 cells were analyzed in presence and abscenece of fentanyl. Cells were treated with 10ug of fentanyl for 24 hours. ACH-2 cells without fentanyl treatment is considered as control. Both control and test were performed in replicates. Total RNA was extracted from both fentanyl-treated and untreated ACH-2 cells miRNA Isolation Kit