GSM8247016: b7_nega_2; Mus musculus; RNA-Seq
Total RNA was extracted from cells with QIAzol reagent and a miRNeasy Micro kit (Qiagen) following the manufacturer's instruction. Full-length cDNA was prepared using a SMART-Seq HT Kit (Takara Bio, Japan) according to the manufacturer's instructions. An Illumina library was then prepared using a NexteraXT DNA Library Preparation Kit (Illumina).