GSM7029060: Rax-GFP ESCs, H3K27ac control Rep 2; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared for sequencing using standard Illumina protocols