Full length 16S sequences of the infant gut microbiome at 6 weeks, 3 months and 6 months of age.
The 16S amplicons were generated with the barcoded Full-Length 16S primers from PacBio and the 2x Roche KAPA HiFi Hot Start Ready Mix.Forward sequence: AGRGTTYGATYMTGGCTCAG     Reverse sequence: RGYTACCTTGTTACGACTTAmplicons were converted to a library with the SMRTBell Express Template Prep kit 2.0The library was sequenced on 1 SMRTCell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 12hs movie time   CCS analysis was done using SMRTLink V10.0 using the following parameters:ccs --min-passes 3 --min-rq 0.999lima --ccs --different --split-bam-named