GSM8403118: KCP_H3K27Ac_CR_; Homo sapiens; OTHER
Organoids were extracted from the matrigel by cell recovery solution, trypsinized into single cell suspension. 500,000 cells were used per CUT&RUN assay. CUT&RUN library was prepared with no more than 10 ng CUT&RUN DNA using NEBNext Ultra II DNA Library Prep Kit (NEB) according to manufacturer's protocol.