Glucokinase deficient strain of M9.2.50 after adaptation to growth on M9 minimal media (glucose) supplemented with kanamycin:biological replicate 1
Genomic DNA extracted using EZNA Bacterial DNA kit from Bio-Tek. 300bp fragments were generated using a COVARIS ME220. Libraries were generated and indexed using the NEBnext DNA ULTRA II kit from New England BioLabs. Libraries were sequenced on the NovaSeq 6000 instrument from Illumina in paired end mode (2 x 150bp).