GSM6602166: groupA Colitis,  scRNAseq; Mus musculus; RNA-Seq
DSS-induced colitis mice were treated with PBS, A. muciniphila secreting extracellular vesicles (SEV) via gavage every day. Mice lymphocytes of colon tissues from each of the indicated group (Colitis and SEV) in were collected. Then CD4+ T cells are isolated from lymphocytes. These cells were submitted for scRNA-seq. Single-cell suspensions (1×10^5 cells/ml) with PBS (HyClone) were loaded into microfluidic devices using the Singleron Matrix® Single Cell Processing System (Singleron). Subsequently, the scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE® Single Cell RNA Library Kits (Singleron ) (B, et al., 2019). Individual libraries were diluted to 4 nM and pooled for sequencing. At last, pools were sequenced on Illumina novaseq6000 with 150 bp paired end reads.