GSM7147039: IFE-1; Mus musculus; ATAC-seq
P60 TNC-CREER; tdTomato back skins were collected and subjected to 0.25% trypsin digestion for 60 minutes at 37ºC with 50 rpm shaking. 1:100 SCA1-Cy7 (Biolegend), 1:100 α6-integrin -FITC (Biolegend) and 1:500 EPCAM-APC (Biolegend) for 30 minutes on ice and washed twice with 1x DPBS before cell sorting. Touch dome cells (TD) was sorted as EPCAM (+), SCA1 (+), integrin α6 (+) and TOMATO (+). IFE were sorted as EPCAM (+), integrin α6 (+), SCA1 ( +) and TOMATO (-). Hair follicle cells were sorted as EPCAM (+), integrin α6 (+), SCA1(-) and TOMATO (-). FACS-purified cells were collected directly into E-media, and cells sample preparation, tagmentation and PCR amplification of tagmented DNA was done using the ATAC-seq kit (Active Motif) . 50,000 FACS-purified cells were collected directly into E-media. Cells sample preparation, tagmentation and PCR amplification of tagmented DNA was done using the ATAC-seq kit (Active Motif).