GSM8246807: finfish liver, Corn Starch; Tank Number - T33; Trachinotus carolinus; RNA-Seq
Two fish from each tank were euthanized with an overdosed of MS-222 at 300 mg/L for dissection of the liver which was immediately stored at – 80 °C for subsequent transcriptomic analysis. Total RNA was extracted from liver samples using RNeasy purification kit (RNeasy Mini kit, ID:74101, QUIAGEN, Venlo, The Netherlands) following manufacturer's protocol. RNA quality, purity and quantity were determined using NanoDrop 1000 based on 260/280 and 260/230 absorbance ratios (Thermo Fisher Scientific, Waltham, MA, United States). Then, RNA samples were sent to GeneWiz (South Plainfield, NJ, USA) for sequencing on an Illumina HiSeq.