GSM7102494: OCEAN-C, HL-60, RA 4h 2; Homo sapiens; Hi-C
Cells were fixed with 1% formaldehyde first and then suspended with lysis buffer. DNA was then digested with MboI enzyme,  and the ends of restriction fragments were filling-in with biotin- labeled dATP followed by re-ligation by the T4 ligase. Then the nucleus were subject to sonication in 2 ml lysis buffer (10 mM Tris-HCl [pH 8.0], 2% Triton X-100, 1% SDS, 100 mM NaCl, and 1 mM EDTA) to get an average DNA fragment size of 300–400 ,bp. The supernatant was then used to get the open chromatin using the zymo DNA extraction kit. To reverse the cross-linking, 1 μl DNase-free RNase A was added to the DNA solution and incubate at 37 °C for 30min. Then 1 μl of proteinase K was added and incubated at 55 °C for 1 h and then followed by incubation at 65 °C overnight. Myone Streptavidin T1 beads (65602) was used to pull down biotinylated DNA. The library construction was then performed on beads following in situ Hi-C protocol. OCEAN-C