GSM7433367: Wild type mouse lung BAL, HTO-derived DNA; Mus musculus; OTHER
mRNA was extracted from mouse BAL single cell suspension using the 10X Genomics Chromium™ Single Cell G Chip Kit Library was prepared using Chromium Single Cell 3ʹ v3.1 protocol (10x Genomics). Cellular mRNA and hashtag antibody-derived oligos (HTO) were reverse-transcribed and indexed with a shared cellular barcode. mRNA derived cDNA and HTO DNA were constructed to libraries according to the Chromium Single Cell 3' v3.1 protocol (10x Genomics). mRNA derived cDNA library and HTO library were pooled and sequenced at 4:1 ratio.