GSM7989695: UTSCC76a; Homo sapiens; RNA-Seq
Single cell suspensions were generated by incubation of spheroids with 0.5% Trypsin/0.5mM EDTA (Gibco). After washing with ice-cold DMEM, single cells were counted using Luna-II automated cell counter (Logos Biosystems) and loaded on a microwell cartridge of the BD Rhapsody Express system (BD) following the manufacturer's instructions. Single cell whole transcriptome analysis libraries were prepared according to the manufacturer's instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles, Illumina) for 2 × 75 bp paired-end reads with 8 bp single index aiming at sequencing depth of >20,000 reads per cell for each sample.