GSM7029206: TL3_Hela_50_45m_rep1; Homo sapiens; OTHER
Labeled cells were washed in ice cold PBS  before being directly scraped in Trizol LS. RNA extraction was performed using Trizol LS and treated by TimeLapse-chemistry (0.1M sodium acetate pH 5.2, 4mM EDTA, 5.2% 2,2,2-trifluoroethylamine, and 10mM sodium periodate at 45°C for 1 hour). SMARTer Stranded Total RNA HI Mammalian kit (Takara)