GSM7817966: 441031; Mus musculus; RNA-Seq
Male C57BL/6 mice (4–5-week-old, Wuxi Apptec, Shanghai, China) were either transduced via the tail vein with 1x1011 viral genome equivalents (vge) per mouse of rAAV-HBV1.3 (n=5)  (FivePlus Molecular Medicine Institute, Beijing, China) or rAAV vector control (n=5), which contained an empty rAAV capsid and rAAV containing a short sequence of the CMV promoter and BGH poly A tail flanked with inverted AAV-ITR or kept naïve (n=5). IHICs isolated via mechanical digestion of the liver were processed according to the 10x Chromium Single-cell V(D)J Reagent Kits User Guide and loaded on to the Chromium 10x platform using the 5' v1.1 chemistry (10x Genomics). Libraries were sequenced on a NovaSeq 6000 platform (PE150) (Illumina) to an average ~50,000 reads per cell. 10x 5'