GSM8062277: 5xFAD 10Hz 4hr Rep1 Hip; Mus musculus; RNA-Seq
RNAwasisolated from mouse brain tissue  tissues using Qiagen RNeasy kit(217804; Qiagen; Hilden, Germany)according to the manufacturer's protocols. Libraries were sequenced for paired-end mRNA using the NovaSeq 6000 sequencing system to obtain a sequencing depth of 30 to 40 million reads per sample. Before sequencing, quality control was performed using a bioanalyzer for RNA integrity number greater than seven. The NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit were used to prepare sequencing libraries.