GSM7324823: Earthworm_whole body, 4 month-old, 2; Eisenia andrei; ATAC-seq
Samples were  subjected to manual pulverization via grinded in liquid nitrogen. The pulverized tissues were aliquoted into  1.5 ml lysis buffer  [10mM Tris-HCL (pH 7.5), 10mM NaCl, 3mM MgCl2, 0.1% Tween-20 (Sangon Biotech), 0.1% IGEPAL-CA630 (Sigma Aldrich) and 0.01% Digitonin (Thermo Scientific) in water], and followed by incubation for 3 minutes on ice for nuclei extraction. Library preparations were performed with the CH ATAC-seq protocol