GSM7816514: MicroC_AML_MA9_rep1; Homo sapiens; OTHER
1x106 leukemia and healthy donor cells were resuspended in 1xPBS and crosslinked with 3mM DSG and 1% formaldehyde. Treated cells were then washed and digested with 0.5 uL MNase in 100 uL of Nuclease digest buffer. Digested chromatin was thawed by addition of 3µl of 20% SDS and capture on chromatin capture beads. Micro-C libraries were performed following Dovetail Micro-C Kit protocol (Cat # 21006, Dovetail Genomics, Scotts Valley, CA, USA)