16S rRNA amplicon sequence from heat treated municipal wastewater sludge
DNA extracted from 0.25 g of sludge using QIAGEN kits. V4 region of 16S rRNA amplified using Klindworth et al (2013) protocol. Successfully amplified products were quantified via qubit fluorescence and sent to the Biorenewables Development Centre in York for sequencing via Illumina MiSeq