GSM7325057: S1; Ginglymostoma cirratum; RNA-Seq
Animals were immunized subcutaneously in the ventral side of the pectoral fin with 300 µg R-phycoerythrin (PE) emulsified in Freund's complete adjuvant, spleens flash frozen immediately for libraries s1 and s2 we followed the nuclei extraction protocol of Taylor & Ruiz Daniels 2022 dx.doi.org/10.17504/protocols.io.261genwm7g47/v2 Nuclei were processed through the 10X Chromium™ Single Cell Platform using the Chromium™ Single Cell 3` Library and Gel Bead Kit v3.1 and Chromium™ Single Cell A Chip Kit (both 10X Genomics) as per the manufacturer's protocol with the aim of recovery of 7000 nuclei.