Plathymenia ddradseq
The genomic DNA was digested with the restriction enzymes EcoRI and MseI, ligated with adaptors with unique barcodes, pooled, and then sizeselected the libraries to 350 to 450bp using the Pippin Prep. The library was sequenced (100-bp single-end reads) on a lane of an Illumina HiSeq 2000 flow cell.