GSM8247176: gata4_rep1_xen; Mus musculus; OTHER
CUT&RUN on Gata4 and Gata6 factors was performed on 500,000 3F2 cells as previously described with minor modifications (Skene et all., 2018). CUT&RUN libraries were prepared using the Thruplex Library Prep kit (Takara) according to the manufacturer's instructions. The number of cycles for library PCR amplification was determined by running 10% of each library sample in an Applied Biosystems qPCR machine with 0.25μl Eva Green dye (Biotium 31000) for 40 cycles and adding cycles to each sample as described (Buenrostro, et al., 2015). Libraries were size selected with 1.5x volume SPRI beads (Beckman Coulter B23317) and sequenced at the Weill Cornell Genomics Resources Core Facility on an Illumina NovaSeq 6000 (PE-50, 30 million reads per sample).