Nano-DMS-MaP of HIV-1 genome: in cell 20mM DMS rep1
transfection of plasmid expressing HIV-1 NL4-3 del Gp, 24 h p.tfx. DMS probing, RNA extraction from cells, RT with RT-FL, PCR with PCR-HIV_FL_B_Fw and PCR-HIV_FL_B_Re, and sequencing with native barcoding kit Nanopore SQK-NBD112.96