GSM8247833: suprachiasmatic nucleus, LONG photoperiod, pZT12, rep9; Mus musculus; RNA-Seq
AllPrep DNA/RNA Micro Kit (Qiagen) Stranded cDNA libraries were constructed using 200 ng of total RNA per sample and the NEBNext® Ultra™ II RNA Library Prep (NEB, Cat: E7765S) per manufacturer's instructions, with mRNA enriched via poly-A-selection using oligoDT beads. Paired-end sequencing produced approximately 54.6 million reads per sample (total of 11.1 billion reads).