GSM7433662: PolyE, Media, replicate 4; Homo sapiens; RNA-Seq
Cells were sorted directly into Buffer RLT (Qiagen) for total RNA extraction with the Rneasy Micro kit (Qiagen). Paired-end libraries were prepared using the Tecan Trio RNA-seq for low-input samples on the Agilent BRAVO. Custom AnyDeplete probes (Tecan) were added against the human mitochondrial genome, HBB, EEFA1, MALAT1, and HSP90aa1.