Illumina RNA-seq of co-purified RNA in sample fraction
Spodoptera frugiperda (Sf9 cell line) telomerase was purified by a two step chromatographic strategy and telomerase activity was followed by Telomere Repeat Amplification Protocol (TRAP) through the purification steps. After the second chromatographic step, RNA co-purifying with peak telomerase activity fraction were extracted and analyzed by Illumina next-generation sequencing (NGS). Briefly Illumina libraries were constructed using the ScriptSeq v2 RNA-seq library preparation kit (Epicentre) and sequenced using the NextSeq 500 instrument. Adapters were trimmed and read pairing was maintained with trimmomatic.