GSM7911355: Isotype_PanCKneg_005; Mus musculus; OTHER
Brain was isolated after PBS and PFA-perfusion and FFPE-brain sections were cut at 5 µm. Samples were further processed by Nanostring (Seattle, United States of America), and the GeoMx DSP slide preparation manual was followed. Probes were hybridized to the analyte of interest on a prepared tissue slide and then loaded onto the GeoMx Digital Spatial Profiler. Within targeted spatial regions, probes' molecular barcodes were UV-cleaved via a digital micromirror device. Molecular tags for each spatially-targeted region of interest were aspirated independently. Each analyte was processed as an independent sample. PCR to amplify molecular tags and add adapters, AMPure bead clean-up