GSM8150786: siPKN1-Static_2; Homo sapiens; RNA-Seq
RNA was extracted from HUAECs using the miRNeasy Micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen). RNA and library preparation integrity were verified using the LabChip Gx Touch 24 (Perkin Elmer). 3µg of total RNA was used as input for Truseq Stranded mRNA Library preparation following the low sample protocol (Illumina). Sequencing was performed on NextSeq500 instrument (Illumina).