Clonal evolution during metastatic spread in high-rish neuroblastoma: Sample I-H-135089-T2-1-R1-1
Tumor RNA was extracted from fresh frozen or OCT tumor biosies. Samples were first homogenized in 1mL TRIzol Reagent (ThermoFisher catalog #15596018) followed by phase separation with 200 μL chloroform. RNA was extracted using miRNeasy Micro Kit (Qaigen catalog #217084) on the QIAcube Connect. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 18ng-11μg of total RNA underwent ribosomal depletion and library preparation using TruSeq Stranded Total RNA LT Kit (illumina catalog #RS-122-1202). Samples were barcoded and run on a Illumina HiSeq 2000, 2500, 4000 or NovaSeq 6000 machines.