GSM6647596: ALD2_1226_scTCR; Homo sapiens; OTHER
Cells were sorted via FACS. Cells were centrifuged for 10 min at 400xg, the supernatant was carefully removed leaving 20 µL for loading on a Chromium Chip K (10x Genomics) according to the manufacturer's instructions for processing with the Chromium Next GEM Single Cell 5′ Library and Gel Bead Kit v2. gene expression libraries were prepared with the Next GEM Single Cell 5′ Library and Gel Bead Kit v2. TCR single-cell libraries were subsequently prepared with the Chromium Single Cell V(D)J Enrichment Kit, Human T Cell. Libraries were sequenced on Illumina NovaSeq 6000 machine with 2x100 bp for gene expression, aiming for 50,000 reads per cell for gene expression and 5000 reads per cell for TCR libraries.