Whole exome sequencing of vestibular schwannomas with matched blood samples.
Genomic DNA was isolated from FFPE tissue and automated dual indexed libraries were constructed with 50-250ng of DNA utilizing the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer) targeting 250bp inserts. 10 samples were pooled at an equimolar ratio yielding ~2.2µg in the pool prior to the hybrid capture. Library pools were hybridized with IDT xGen™ Exome Hyb Panel v2 probes. Library concentration was quantified and libraries loaded on a NovaSeq S4 flow cell targeting 200x coverage with 2x150bp reads. All sequencing was completed according to the manufacturer's recommendations (Illumina Inc, San Diego, CA).