Whole genome PSS of 135 IMR90 Cells
Fixed wells of IMR90 cultured cells underwent a gentile histone denaturing treatment (0.1N HCl for 5 minutes at room temperature), and were tagmented in situ using Tn5 Transposase loaded with blocked adapters. The fragments from variable numbers of nuclei were uncaged using targeted illumination. Uncaged fragments were amplified and sequenced on an Illumina platform.