GSM7328328: MCF7.Control.2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA was assessed using the TapeStation (Agilent) and Qubit RNA broad-range assay (Thermofisher). Samples with RINs (RNA Integrity Numbers) of 8 or greater were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), xGen Broad-range RNA Library Prep (IDT), and xGen Normalase UDI Primer Plate 2 (IDT). 820ng of total RNA was converted to mRNA using polyA purification. Final libraries were checked for quality and quantity by Qubit hsDNA (Thermofisher) and LabChip (Perkin Elmer).