GSM6603410: BL4; Mus musculus; RNA-Seq
RNA was extracted using NucleoSpin RNA kit from Macherey-Nagel Ribosomal RNA was depleted using Illumina Ribo-Zero Plus rRNA Depletion kit protocol (catalog no. 20037135).  Libraries were prepared by Illumina TruSeq Stranded Total RNA Library Preparation Kit protocol (catalog no. 20020597).  The libraries were analyzed by Agilent 4200 TapeStation, and pooled.  The pooled libraries were loaded on a NextSeq 500/550 High Output v2.5 run with a 75-cycle sequencing module (catalog no. 20024906) to generate paired-end reads.  The demultiplexing of the reads was performed using bcl2fastq version 2.20.0.