GSM7434186: CVG, Pou4f1KO, E11.5; Mus musculus; ssRNA-seq
Total RNA was extracted from fresh frozen tissue using Trizol following manufacturer's instructions (ThermoFisher Scientific, Waltham, MA, USA). Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA).