DICER-Ko BB-SAM1 RNAseq
293T and DICER1-Ko cells transfected in triplicates with either BB-SAM or 759-SAM and grown for for 72 hours. Total RNA was extracted using the miRNeasy Kit (Qiagen, Germantown, MD, USA) and treated with RNase-free DNase (Qiagen) according to the manufacturers instructions. 0.1 g total RNA was used for stranded total RNA library preparations (Illumina TruSeq, Cat#_20020596). This workflow included a Ribo-Zero Human/Mouse/Rat RNA depletion step. Libraries were prepared with unique barcodes and pooled at equalmolar ratios. The pool was denatured and sequenced on Illumina NextSeq 500 sequencer using high output V2 reagents and NextSeq Control Software v1.4 to generate 75 bp paired-end reads, following manufactures protocol (Cat# 15048776 Rev.E).