GSM6798211: 2153L GEMM TNBC Combo, Input, biol rep 1; Mus musculus; RNA-Seq
Input and dsRIP RNAs were extracted using Direct-zol RNA Microprep Kit (ZYMO Research, R2060). The antibodies used in the study were manufactured by Exalpha Biologicals, Inc. with catalog number 10010500. RNA libraries for RNA-seq were prepared using Illumina Stranded Total RNA Prep with Ribo-Zero Kit (Illumina, 20040525) following Illumina's recommended protocol. First stranded cDNA synthesis was performed using SuperScript III (ThermoFisher, 18080044) with the following modifications: RNA was heated to 70°C for 3 minutes to reduce secondary structure, followed by reverse transcription for 10 minutes at 25°C, 50 minutes at 50°C, and 15 minutes at 70°C. Amplified libraries were purified and quantified using the KAPA quantification kit (Roche).