GSM7103248: p7_bra; Mus musculus; miRNA-Seq
Trizol protocol Total RNA and miRNAs were extracted simultaneously with NucleoSpin® miRNA columns (Macherey-Nagel, Düren, Germany), according to the manufacturer's instructions Indexed cDNA libraries from the RNA fraction at low molecular weight were prepared using the TruSeq small RNA sample Preparation kit (Illumina, San Diego, CA) according to the manufacturer's protocol and recommendations. Single end sequencing (1 × 50b), after fluorimetric quantification, was performed on an Illumina Hiseq II platform.