GSM6798029: Proximal colon on plenish + coconut oil 1; Mus musculus; RNA-Seq
At the end of the study animals were euthanized by CO2  inhalation followed by cervical dislocation. Intestinal tissue was excised immediately and put in RNALater for 24 hours at room temperature and then stored at -80°C. Total RNA was isolated using miRNeasy kit (Qiagen, Inc., Valencia, CA) and evaluated for purity and concentration by NanoDrop (Wilmington, DE) and Agilent 2100 Bioanalyzer (Santa Clara, CA). 4 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TruSeq Long RNA Sample Prep Kit following manufacturer's protocols.