GSM7990809: DU145, GEX; Homo sapiens; OTHER
After 24 hours from treatment, the cells were harvested for the single-cell RNA-ATAC Co-assay. The library construction was following the standard 10X protocol with the following modifications to the protocol:  1) 1µl of 10µM HTO additive primer was spiked in during the pre-AMP and cDNA PCR steps; 2) during the 0.6X cDNA cleanup, the supernatant was saved to amplify the HTO library. Along with the transcriptome library, the HTO library was amplified from the supernatant using the HTO-specific primer. The detailed protocol can be found on the cell hashing website (https://cite-seq.com/cell-hashing/).