GSM7597605: Heme 3; Mus musculus; RNA-Seq
RNA was extracted from cells using the RNeasy Micro kit (Qiagen) according to the manufacturer's protocol, including on-column DNase I treatment. RNA quality was validated an Agilent Technologies 2100 Bioanalyzer using an RNA chip, and only samples with an RNA integrity number (RIN) of > 9 were used for sequencing. cDNA libraries were generated from the RNA samples using the Illumina TruSeq RNA stranded kit following the manufacturer's instructions. Libraries were amplified by PCR (total of 15 cycles) and their quality and concentration determined using an Agilent Fragment Analyzer with DNA High-Sensitivity Chips. The libraries were pooled in equimolar amounts.