GSM7433603: filamentous fungus reference strain, glucose, rep2; Trichoderma reesei RUT C-30; RNA-Seq
8 mL samples were filtered and immediately frozen in liquid nitrogen. Frozen mycelia were ground by a Fastprep homogenizer for 2 x 40 sec at 6 m/s using Lysis matrix C (MP Bio) with 700 µL of RTL Buffer (Qiagen RNeasy Mini Kit) and 7 µL of β-mercaptoethanol. After removing cell debris by a QIAshredder column, RNA was extracted using the RNeasy Mini Kit and following the instructions of the manufacturer (Qiagen). The RNA concentration was measured with Qubit 2.0, and the quality of the extracted RNAs was evaluated on a Bioanalyzer (Agilent) and agarose gel electrophoresis. Library preparation and Illumina sequencing were performed at the Ecole normale superieure genomics core facility (Paris, France). Messenger (polyA+) RNAs were purified from 1000 ng of total RNA using oligo(dT). Libraries were performed using the strand specific RNA-Seq library preparation Stranded mRNA Prep, Ligation kit (Illumina) and were multiplexed on two P2 flowcell. (Illumina). A 118 bp single-end read sequencing was performed on a NextSeq 2000 device (Illumina). A mean of 42 million  ± 4 million passing Illumina quality filter reads was obtained for each of the 16 samples