GSM8324923: Spleen of vavP-Bcl2 mouse #1; Mus musculus; RNA-Seq
Spleens and spleen-derived tissue explants were mechanically disaggreagted using 70mm strainers, erythrocytes were lysed using a Red Blood Cell Lysis buffer for 2 minutes at room temperature, and then cells were washed in PBS, resuspended in medium and processed for sequencing. Samples were processed for sequencing using the SingleCell 3' Reagent Kit v3.1 and a 10x Chromium single cell controller following the manufacturer's instructions.