GSM8246822: AC16 cells, cell-L-Ang1; Homo sapiens; RNA-Seq
Total RNA was extracted using miRNeasy Micro Kit (Cat #217084, Qiagen), and RNA quality was checked using Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US) according to standard procedures.The exosomes were isolated and total RNA was extracted using exoRNeasy Maxi Kit (#77164, Qiagen), and RNA quality was checked using Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US) according to standard procedures Sequencing libraries were constructed by VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Cat #NR604, Vazyme) according to manufacturer's instructions. In exosomes, sequencing libraries were constructed by SMARTer Stranded Total RNA-Seq Kit for Illumina (Cat#634414, Takara) according to manufacturer's instructions.