DNA-Seq of Homo sapiens: juvenile female peripheral blood
Genomic DNA (1 g) extracted from peripheral blood was hybridized and enriched. The DNA fragments were then end repaired, and the 3end was added one A base. Secondly, the DNA fragments were ligated with barcoded sequencing adaptors, and fragments about 320 bp were collected by XP beads. Subsequently, the libraries were quantified by qPCR and size distribution was determined using an Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Finally, Novaseq6000 platform (Illumina, San Diego, USA), with 150 bp pair-end sequencing mode, was used for sequencing the genomic DNA of the family.