GSM8195831: BLaER subclone CTCF-AID, 96h_CTCF_depl_rep2; Homo sapiens; RNA-Seq
Cells were collected, centrifuged at 200g, 5 min, then lysed with Qiazol. RNA extraction was performed using standard chroroform-isopropanol extraction method. 150ug of RNA for each condition was diluted to 130 uL, sonicated using Covaris (100W intensity, 1% duty cycel, 10s). For each sample 2 uL of sonicated RNAs is used for RNA clean up with miRNeasy micro kit (Qiagen), followed by DnaseI digestion. Library prep is used with Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit, and indexed with Illumina UD Indexes, then sequenced with Nextseq 2000.