Ribo-seq-rnaseq-Psph-WT-1
Samples were first cultured to the stationary phase in KB at 28C. Cultures were collected, and total RNA was extracted using a RNeasy mini kit (Qiagen) for subsequent purification with DNaseI (NEB) treatment. After removing rRNA by using the MICROBExpress kit (Ambion), mRNA was used to generate the cDNA library according to the NEBNext UltraTM II RNA Library Prep Kit protocol (Illumina), which was then sequenced, generating 150-bp paired-end reads. Two biological replications have been performed.