GSM7432842: 2D 3T3-L1 DIF- (2); Mus musculus; RNA-Seq
Total RNA was extracted using an RNeasy mini kit (Qiagen, Valencia, CA). Reverse transcription was performed with the SuperScript IV kit (Invitrogen) according to the manufacturer's instructions. Libraries were prepared for sequencing using library creation kits (NEBNext Ultra Directional RNA Library Prep Kit, Illumina, San Diego, CA, USA)