The bacterial community in water samples collected from an inflow river of Taihu Lake, China
The V3 and V4 regions were amplified using forward primers containing the sequence CCTACGGRRBGCASCAGKVRVGAAT and reverse primers containing the sequence GGACTACNVGGGTWTCTAATCC. Indexed adapters were added to the ends of the 16S rDNA amplicons. DNA libraries were validated by Qubit3.0 Fluorometer. Sequencing was performed using paired-end on an Illumina MiSeq platform