DICER-Ko BB-SAM1 sRNAseq
293T and DICER1-Ko cells transfected in triplicates with either BB-SAM or 759-SAM and grown for for 72 hours. Total RNA was extracted using the miRNeasy Kit (Qiagen, Germantown, MD, USA) and treated with RNase-free DNase (Qiagen) according to the manufacturers instructions. The RNA were organized in one batch of 12 samples, each containing 1.0 g total RNA. For cDNA library preparation, 2.5 fmoles of Calibrator Set2 (standard calibrator), a set of ten 21-nt 5-phosphorylated RNA oligos, was added. Each RNA sample was individually 3-adapter-ligated. Up to 24 reaction products were pooled, 5 adapter-ligated, PCR-amplified and sequenced in a single NextSeq500 lane.