control 2 hour treatment rep 1
Col-0 was grown vertically for 7 days on MS/2 media and incubated in water or 50 mM DA for 2 or 8 hours. The tissue was washed, blotted dry, weighed, and snap-frozen in liquid nitrogen. RNA extraction, library construction, and sequencing were carried out by Novogene (https://novogene.com). In brief, mRNA was extracted and purified using poly-T oligo-attached magnetic beads and fragmented. First strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Library then underwent end repair, A-tailing, adapter ligation, size selection, amplification, purification, and sequencing using the Illumina Hiseq 2000 platform.