GSM6645310: G6R2TA; Rattus norvegicus; RNA-Seq
RNA extraction from adipose tissue samples was carried out according to the protocol proposed  in the RNeasy Lipid Tissue Mini Kit The sequencing library was prepared by  random fragmentation of the cDNA, followed by the 5 'and 3' ligation adapters. Adapter-ligated fragments were amplified by PCR and visualized on agarose gels. Double stranded next generation  sequencing was commercially performed by MAcrogen (Seoul, Korea)