GSM7989928: Islet_100, SAMN14217400, 24 Hr Cytokines; Homo sapiens; RNA-Seq
Total RNA was extracted and purified from 2,000–3,000 IEQs using TRIzol (Life Technologies). ERCC Mix 1 or Mix 2 spike-ins were then randomly added to each sample (Thermo Fisher, catalog #4456740). Human islet RNA-seq libraries were prepared from total RNA using the stranded TruSeq kit (Illumina).