RNA-Seq of Shewanella putrefaciensCN 32: Cu_PFS_2 reduction
The cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to the Illumina protocol. Libraries were constructed by PCR amplification for 15 cycles, and quantified by TBS380, followed by 150 bp*2 paired-end sequencing on Illumina Novaseq 6000 by Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. (Shanghai, China)