GSM7508855: SHAM1_day21_biol_rep_1; Mus musculus; RNA-Seq
RNA was isolated using TRIzol reagent and ~4 ug was submitted to LC Sciences (Houston, TX, USA) for Sequencing Poly(A) RNA-seq library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol (Illumina, San Diego, CA, USA) after passing RNA integrity checks (RIN>7). Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads in two rounds. Quality control and sequencing library quantification were performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip.