GSM7053621: scRNAseq, patchseq, cortical IN,  47; Mus musculus; RNA-Seq
After whole-cell recording, nuclear and cytoplasmatic RNA from somatosensory cortex Htr3a-GFP+/Hmx3-Cre;R26tdTOM+ cells from mice between P14 and P25 was collected into the recording pipette by applying light suction. Pipette contents were transferred to RNAse-free PCR tubes containing nuclease-free water, lysis buffer from the SMART-Seq V4 3' DE Kit and RNase inhibitor. Collected single cells were flash-frozen in dry ice and stored at -80°C until further processing.