GSM7326246: Calibrated Scc1 ChIP-seq for wild type INPUT; Schizosaccharomyces pombe; Saccharomyces cerevisiae; ChIP-Seq
Beads and input samples were resuspended in elution buffer (50 mM Tris–HCl pH 7.5, 1% SDS, 10 mM EDTA) and incubated at 65°C for 2 h on a thermal shaker. Next, beads were removed and the IP eluates and input samples were  incubated at 65°C overnight. Next day, all the samples were treated with RNAse A for 1 h at 37°C followed by 2h incubation at 65°C in the presence of Proteinase K. Finally, DNA was recovered using a Monarch PCR & DNA Cleanup Kit (New England BioLabs, T1030L). DNA libraries were constructed using NGS DNA Library Prep Set (Novogene, PT004).