GSM7052540: scRNAseq, POA E14.5 Htr3a-GFP+/Hmx3-Cre;R26tdTOM+  precursor 355; Mus musculus; RNA-Seq
Htr3a-GFP/Hmx3-Cre;R26tdTOM E14.5 brains were extracted in ice-cold HBSS. After sectioning with a Mcllwain tissue chopper, POA was micro-dissected under a stereomicroscope and incubated in 0.05% trypsin at 37°C for 5 minutes. After digestion, FBS was added and cells were mechanically dissociated via pipetting and centrifuged 5 minutes at 300g. Cell pellet was then suspended in 1ml HBSS and filtered using a 70um strainer. Htr3a-GFP+/Hmx3-Cre;R26tdTOM+ cells were FAC-sorted on a MoFloAstrios device gated for selecting GFP+/TOM+ cells. Sorted cells were captured using integrated fluidic circuits and imaged prior to sequencing.