GSM8247921: post-traumatic arthrosis of the 2nd degree before laser exposure; Homo sapiens; RNA-Seq
Synovial fluid was mixed with phosphate buffered saline. Cells were pelleted by centrifugation and frozen in 90% fetal bovine serum and 10% dimethyl sulfoxide. On the day of the experiment, the cells were thawed, their viability was assessed, and a protocol for RNA extraction and library preparation was carried out. Library preparation was carried out according to the Chromium Next GEM protocol using Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) CG000315 Rev E