GSM6797832: Kras mutant, 1; Danio rerio; RNA-Seq
Total RNA was extracted from individual micro-dissected zebrafish livers using the RNeasy Micro Kit (QIAGEN, #74004). RNA (100 ng per sample) was used for RNA-sequencing library preparation using the TruSeq RNA Sample Prep Kit with Ribo-Zero depletion (Illumina). Indexed libraries were sequenced on a NextSeq 500 instrument (Illumina) to generate 80 bp paired end reads and yielding at least 20 million reads per sample.