GSM7817755: Infected-BMDM Rep3; Mus musculus; RNA-Seq
Bone marrow macrophages (BMDM) from C57BL/6 mice were cultured for 24h with Trypanosoma cruzi (Tulahuen Strain, TcVI). Cell to parasite ratio was set to 1:3. Then, 2x106 BMDM were pelleted, lysed, and RNA was harvested according to the Qiagen RNeasy Mini Kit protocol. Only Samples that passed the purity cutoff (Abs 260/Abs 280 = 1,7-2) were selected for downstream processing. Libraries were processed by the Broad Institute's Broad Technology Labs and the Broad Genomics Platform. Libraries were prepared according to the 3' Digital Gene Expression (3' DGE) protocol (Soumillon et al., 2014). RNA was sequenced at the Broad Technology Services using the High-throughput eukaryote 3' DGE library.