GSM7789846: E-Cn2; Mus musculus; RNA-Seq
After the interaction, the macrophage monolayer was washed, and total RNA was isolated using the RNAeasy kit (Qiagen) following the manufacturer's protocols. RNA quantification and integrity were assessed using the Agilent 2100 Bioanalyzer. Polyadenylated RNA-enriched libraries were prepared using the TruSeq Stranded mRNA Sample Prep Kit (Illumina) following the manufacturer's protocols.