GSM8402800: Primordial granulosa (MS234_030); Mus musculus; RNA-Seq
For the bulk RNA-seq samples, the mouse gonadal/ovarian cells were lysed and the total RNAs were purified using a NucleoSpin RNA XS (Takara Bio, U0902A) according to the manufacturer's instructions. Biologically duplicated (E9.5, E10.5, E11.5, E12.5, E13.5, E16.5, E17.5, E18.5, P0, P1, ESCs, EpiLCs, c0, c3 , c5 ,c7, c11, c13) or triplicated (E14.5,E15.5) samples were prepared in each stage.1 ng of total RNAs from each sample was used for the synthesis and amplification of cDNAs. The cDNA synthesis and library construction were as described previously (Nakamura et al, 2015; Ishikura et al, 2016; Okamoto et al, 2021). The sequencing was performed on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina). For the SC3-seq samples, dissociated oocytes and granulosa cells from in vivo mouse ovarian follicles and in vitro oocyte-like cells were manually picked up using glass capillaries under a stereomicroscope (Leica, S8 APO).  The subsequent cDNA synthesis and amplification methods were as previously described (Kurimoto et al, 2006, 2007; Nakamura et al, 2015, 2016). and the 3' termini of cDNA were subjected to sequencing on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina). For the bulk RNA-seq samples, 1 ng of total RNAs from each sample was used for the synthesis and amplification of cDNAs. The cDNA synthesis and library construction were as described previously (Nakamura et al, 2015; Ishikura et al, 2016; Okamoto et al, 2021). The sequencing was performed on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina). For the SC3-seq samples, the subsequent cDNA synthesis and amplification methods were as previously described (Kurimoto et al, 2006, 2007; Nakamura et al, 2015, 2016). and the 3' termini of cDNA were subjected to sequencing on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina).