GSM6744210: TRF1_ZBP1 WT_1; Homo sapiens; RNA-Seq
Total RNA was isolated using TRIzol (Invitrogen #15596018) and purified with RNAeasy Mini Kit (Qiagen #74106). The quality of the isolated total RNA was assessed using Agilent TapeStation 4200. Libraries were prepared with 500ng total RNA using the TruSeq stranded mRNA sample preparation kit according to the manufacturer's protocol (Illumina). RNA-seq libraries were multiplexed, normalized, and pooled for sequencing. The libraries were sequenced on the HiSeq 4000 system (Illumina) at single read 50bp or NextSeq 500 system (Illumina) at single read 75bp.