GSM7061750: Homo sapiens MNase digestion products; synthetic construct; MNase-Seq
Around 1μg of DNA was isolated from each MNase reaction using a Monarch PCR & DNA Cleanup Kit (elution volume of 15 μl, NEB). The DNA was treated with 5 U of Antarctic Phosphatase (NEB #M0289S) for 1h at 37°C in 20 μl reactions. The phosphatase was heat-inactivated for 2 min at 37°C. Libraries were constructred using the Azenta Amplicon-EZ service