GSM7101307: LepR+ cells, SCG, biol rep 1; Mus musculus; RNA-Seq
Single cell suspensions were prepared from the Superior Cervical Ganglia as above using 10wo LepReYFP mice. Live, LepReYFP+ cells were sorted using a FACSAria IIu high-speed cell separator. cDNA libraries were prepared according to the Smart-Seq2 protocol with the following adaptations: addition of 1mg/ml ultrapure BSA to lysis buffer, addition of 20μM template strand exchange nucleotide. cDNA library quality was checked and samples were subjected to paired indexing sequencing.