GSM7949353: Control, 1h, biol rep 1; Homo sapiens; RNA-Seq
RNA was extracted from pelleted cells using a Gen EluteTM Total RNA Purification kit (Sigma Aldrich, Poole, UK) according to the manufacturer's instructions. RNA concentration and purity were determined using a Nanodrop ND-1000 (ThermoFisher Scientific, Loughborough, UK). All RNA samples were stored at -80°C until use. cDNA was generated using a Vilo cDNA Synthesis Kit (Invitrogen, Paisley, UK) according to manufactures instructions and using a VeritiTM 96-well Fast Thermal Cycler (ThermoFisher, Loughborough, UK).