RNA-seq of human CD34+ cells with and without exposure to IFNg
Total RNA was extracted from human CD34+ cells with and without IFN gamma exposure for 48 hours (3 paired samples each) and quantified by nanodrop and analyzed using Agilent bioanalyzer for quality. Single-strand DNA circles and DNA nanoball production by rolling circle replication generated compatible libraries of sequences close to the 3' end of polyadenylated RNA. Libraries were sequenced at 30M paired-end, 150 base pairs using DNBSEQ platform system by BGI, USA.