WGBS of goose leg muscle
DNA conversion and sequencing: We performed bisulfite modification of 500 ng of DNA from each sample with the EZ DNA methylation kit (Zymo Research, Orange, CA).Each sample was bisulfite modified with the EZ DNA methylation kit (Zymo Research, Orange, CA) using 500 ng DNA.Sequencing and data processing of bisulfite were carried out as described previously. The DNA was fragmented using sonication into 100-500 bp size fragments, followed by blunting and dA addition at the 3'end, followed by adapter ligation.In order to monitor the bisulfite conversion efficiency, the adapter sequence contained multiple methylcytosines.An altered protocol from that previously described was used to convert unmethylated cytosines to uracils following bisulfite treatment.Gel-purified DNA fragments with sizes ranging from 320 to 380 base pairs were sequenced according to manufacturer's instructions. DNA that had been converted was sequenced by Illumina Solexa GA on an Illumina system (Illumina, CA, USA) using 50-bp paired ends. Raw data was processed using the Illumina Pipeline v1.3.1.