GSM6602204: LNM(-)-5; Homo sapiens; miRNA-Seq
The isolation of plasma exosomes was conducted using an exosome isolation kit (System Biosciences) according to the manufacturer's instructions. Then, total RNA was isolated from  plasma exosomes by a miRcute miRNA Isolation Kit 3 μg of RNA was used as input material for the generation of a small RNA library. Then, the small RNA library was sequenced on an Illumina HiSeq 2500 platform. Differential expression analysis was performed using the DESeq R package (3.0.3).