GSM7818254: 2D culture, replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted from 2D and MPS cultures using the  RNAeasy Kit (Qiagen, Venlo, The Netherlands) according to the manufacturer specifications. For MPS cultures the collagen matrix, with embedded RPTEC tubules, was dissolved in RTL buffer prior to isolation with mini-spin columns. RNA quantity was evaluted usnig a NanoDrop 2000 (ThermoFisher) Ribosomal RNA was depleted using the RiboZero Kit (Illumina Inc., San Diego, USA) according to the manufacturer's instructions. Library preparation and Illumina dye sequencing was done by Novogene Europe(Cambridge, UK) using a  (Illumina Inc., San Diego, USA) was used for data acquisition.