GSM7432814: D28_1; Mus musculus; RNA-Seq
Plasmodium-specific CD4+ T cells (PbTII) cells from the spleen were isolated by flow cytometry into 1% BSA/PBS buffer. Ten recipient mice were pooled at each timepoint for isolating splenic PbTII cells. PbTII cells were then sorted as single-cells using the Chromium 10x Genomics platform. Approximately 8,000 cells were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 10x Genomics Single Cell 3' Reagent Kit v3. Sequencing libraries were prepared using Single Cell 3' Reagent Kits v3 (10x Genomics).