GSM7102591: L3 animals with dat-1 NanoDam and EGL-43::GFP, replicate 3; Caenorhabditis elegans; OTHER
gDNA was harvested using a Qiagen DNeasy Tissue and Blood kit (#69504) with no deviations from standard protocols. DamID amplicons were obtained as previously described (Gomez-Saldivar et al., 2021) using 4 initial PCR cycles followed by 19 additional cycles. DamID amplicons were directly barcoded through blunt end ligation and sequenced using Oxford Nanopore library reagents (LSK-109, NBD-104, NBD-114)