Targeted amplicon sequence of gut microbiome from Globitermes sulphureus
gDNA of gut microbiome was extracted using stool DNA extraction kit and submitted for Illumina sequencing. PCR amplified targeted amplicon region and Miseq platform prepared the amplicon library. QIIME2 analysed the raw fastq files and trimmed low quality reads using Trimmomatic. Short, paired end reads were merged using FLASH and clustered according to Operational Taxonomic Unit (OTU) with 97% similarity cut-off against Silva (SSU123).