GSM7990248: ChIP_DLD1-NT_H3K9me3; Homo sapiens; ChIP-Seq
ChIP assays were carried out as described previously (Scelfo et al., 2019). Briefly, 1% formaldehyde cross-linked chromatin was sonicated to an average size of 300–600 bp and quantified. 100 μg of sonicated chromatin was incubated overnight at 4°C with 2 μg of the indicated antibodies, and recovered by Sepharose protein A beads. DNA from washed beads was column-purified, quantified using the Qubit dsDNA HS, and used for library preparation with KAPA UDI Primer Mixes Kit.