GSM8403154: CRISPR_screen_low_fluorescence_intensity; Homo sapiens; OTHER
At 5 d post puromycin selection, cells were treated with doxycycline for 48 h. Next, 1.5 × 108 cells were sorted by flow cytometry sorting and collected 5% in high-VENUS-signal cells and low-VENUS-signal cells. Genomic DNA was isolated using a Quick-gDNA MidiPrep (Zymo Research, USA). sgRNA after PCR amplification was sent to the GENEWIZ (Suzhou, China) to be sequenced on Illumina HiSeq 4000 platform on PE150 mode.