WGS of RPE-1 p53KO cells long term treated with reversine 2
After PicoGreen quantification and quality control by Agilent TapeStation, 500 ng of genomic or micronuclear DNA were sheared using a LE220-plus Focused-ultrasonicator (Covaris catalog # 500569) and sequencing libraries were prepared using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with modifications. Briefly, libraries were subjected to a 0.5X size selection using aMPure XP beads (Beckman Coulter catalog # A63882) after post-ligation cleanup. Libraries were not amplified by PCR and were pooled equivolume and quantitated based on their initial sequencing performance. Samples were run on a NovaSeq 6000 in a PE150 run, using the NovaSeq 6000 S4 Reagent Kit (300 cycles) (Illumina). The average coverage per sample was 21X.