Solanum lycopersicum raw transcriptome sequence reads
Leaf samples were collected in commercial tomato and pepper fields in East, Middle and West Tennessee during May through August of 2019. Tissue from individual plants was pulverized in liquid nitrogen. Total RNA was extracted from samples individually. Equal volumes of total RNA from 106 tomato samples were combined to make pool. The pooled of total RNAs was treated with DNase and depleted of ribosomal using a Ribozero Plant Kit. The RNA-seq libraries were prepared with the TruSeq Stranded mRNAseq Sample Prep Kit and sequenced on one lane for 151 cycles from each end of the fragments on a NovaSeq 6000.