Colobanthus quitensis Genome sequencing, 10X chromium
The gDNA was extracted using the QIAGEN MagAttract HMW DNA kit (QIAGEN, Germantown, MD, USA) according to the manufacturers protocol. The quality and quantity of the gDNA were analyzed using a 5400 Fragment analyzer (Agilent Technologies, Santa Clara, CA, USA) and Qubit 2.0 Fluorometer (Invitrogen, Life Technologies, Carlsbad, CA, USA). DNA libraries for samples were generated using the 10 Genomics Chromium technology according to the manufacturers instructions. Gel bead-in-emulsions (GEMs) were created from a library of Genome Gel Beads combined with 1.5 ng of gDNA in a Master Mix and partitioning oil, using the 10 Genomics Chromium Controller instrument with a micro-fluidic Genome chip (PN-120257). The GEMs were then subjected to an isothermal incubation step. Barcoded DNA fragments were extracted and underwent Illumina library construction, as detailed in the Chromium Genome Reagent Kits Version 2 User Guide (PN-120258). Library yield was measured through the Qubit dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, USA). Library fragment size and distribution were measured using an Agilent 2100 Bioanalyzer High Sensitivity DNA chip (Agilent, Santa Clara, CA, USA).