GSM6704960: Day28,ReprogIII; Homo sapiens; Bisulfite-Seq
Human CD8+ T cells were sorted on days 0 (naïve), 7, and 28, and genomic DNA was extracted and bisulfite-treated to convert unmethylated cytosine to uracil residues using the EZ DNA Methylation-Direct Kit (Zymo). DNA libraries were prepared using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the manufacturer's protocol. The libraries were purified by the AMPureXP Beads followed by QC using Qubit method, and the purified DNA was captured on an Illumina flow cell for cluster generation.