WGS Anabrus simplex (Mormon cricket) Colorado adult female, PacBio HiFi 12 cells
High molecular weight (HMW) DNA extraction was performed from the thorax and abdomen of a single Mormon cricket (iqAnaSimp2) using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 2 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 2.0. The library was size-selected with beads to remove library molecules less than 3 kb in length to generate the final libraries for sequencing. Sequencing was performed on a Sequel II System using Binding Kit v2.0, Sequencing kit v2.0, and SMRT Cell 8M.