GSM6705296: EA3_Alectinib_3D; Mus musculus; RNA-Seq
RNA was isolated using Zymo quickRNA Miniprep Kit (cat #11-328) Libraries were constructed using an Illumina TruSEQ stranded mRNA Sample Prep Kit (Cat# RS-122-2101). Total RNA was combined with RNA purification beads to bind PolyA RNA to oligodT magnetic beads. mRNA was eluted and converted to double stranded DNA. A-tailing, adapter ligation, and PCR amplification using 15 cycles was performed to complete the library construction. 2x151 bp reads were generated