GSM7102409: C3H10T1/2 MSCs, input, shControl, day 0; Mus musculus; ChIP-Seq
Cells were cross-linked with 1% formaldehyde (Sigma). Chromatins ranging from 200 and 700 base pairs were obtained following standard ChIP protocol. Chromatin (300 – 400 µg) was precleared with Protein A/G (Pierce) and incubated overnight with 8 µg of NFIB (Atlas, HPA003956), 8 µg of MLL1 (Bethyl, A300-086A), or 4 µg of H3K4me3 (Abcam, ab8580) antibodies. Immune complexes were reverse-crosslinked overnight. The resulting samples were treated with 10 µg of RNase A (Invitrogen) and 20 µg of Proteinase K (Invitrogen) and then ChIP'd DNA was purified with phenol-chloroform solution (Sigma). The integrity of genomic DNA was assessed by an Agilent Bioanalyzer 2100 (Agilent Technologies). The libraries were constructed using a TruSeq ChIP library preparation kit (Illumina).