GSM6645274: male HFD urothelium 4; Mus musculus; RNA-Seq
The NucleoSpin RNA Plus XS Kit was used to extract RNA on enriched IC and PC fractions following the manufacturer's instruction, with one modification: DNase digestion was performed in solution using DNase I. Urothelium samples were isolated mechanically from mouse bladders and RNA was isolated using Qiagen RNeasy Miniprep kit. RNA integrity was measured using Agilent 2100 Bioanalyzer with RNA pico chip. Samples with RNA integrity scores > 7 were used for RNA sequencing. 1 ng of RNA was preamplified using the Takara Clontech SMARTer v4 kit and RNA sequencing libraries were generated with the Illumina Nextera XT DNA Library Prep Kit. Samples were sequenced to a depth of 15–20 million 2 × 150 bp clusters on the Illumina HiSeq 4000 platform.