GSM8421853: breastmilk_donor4_scRNAseq; Homo sapiens; RNA-Seq
cells were sorted into 384-wells plates (Biorad) with 5 μl of vapor-lock (QIAGEN) containing 100-200 nl of RT primers, dNTPs and synthetic mRNA Spike-Ins. Plates were immediately spun down after sorting and frozen to −80°C. Cells were prepared for SORT-seq as previously described. Illumina sequencing libraries were prepared with the TruSeq small RNA primers (Illumina) and sequenced paired-end at 75 basepair read length with 75.000 reads per cell on a NextSeq500 platform (Illumina). Sequencing reads were mapped against the reference human genome (GRCh38) with BWA