GSM8325196: non-diabetic wound [SC_W5]; Homo sapiens; RNA-Seq
Single-cell suspensions were generated from the diabetic and non-diabetic wounds using 10x genomics platform. Briefly, the tissue was chopped into small pieces and subjected to enzymatic dissociation using a whole-skin dissociation kit (cat. no. 130-101-540) in MACS C Tubes (cat. no. 130-093-237; Miltenyi Biotec, San Diego, CA). The samples were incubated in a water bath at 37°C for 3 h. After incubation, the samples were diluted by adding 0.5 mL of cold complete cell culture medium to stop the enzymatic reaction. The C Tubes were then tightly closed and attached upside down onto the sleeve of the gentle- MACS Octo Dissociator, and a UW human skin dissociation program was performed. The cell suspension was subjected to a 70-µm filter and placed on a 15-mL tube to remove the debris. RBCs were lysed using 10X RBC lysis buffer (cat. no. 420301; BioLegend, San Diego, CA). library construction was done according to the manufacturer's recommendations  (10X Genomics)