GSM7597905: SGI-1776-VHL-02 3750 nM Rep 2 (2); Homo sapiens; RNA-Seq
Total RNA was extracted with the Rneasy kit (Qiagen) according to the manufacturer's protocol. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using randomhexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library ordTTP for non-directional library.