GSM8150803: Ty2 LB time 0, rep 1 [Barseq3]; Salmonella enterica subsp. enterica serovar Typhi str. Ty2; Tn-Seq
DNA was extracted using a Qiagen gDNA kit using standard procedures. Purified gDNA was subjected to a barcoding PCR using Q5 polymerase, with a universal P5 primer and unique P7 for each condition, according to published protocols in Wetmore et al., 2015 mbio. This PCR only captures the barcoded region of each Tn insertion.