DNA metabarcoding of Lagopus muta: feces
DNA isolated from fecal samples using the MP Biomedicals FastDNA SPIN kit for soil, and the ITS2 region of rDNA for plants was amplified using the ITS-S2 and ITS4 primers. Libraries were indexed at the 5' and 3' ends of the amplicons using IDT for Illumina DNA/RNA UD indices according to the manufacturer's instructions. Magnetic beads were used to remove fragments under 200bp and over 600bp in length.