GSM6645257: Sublibraries-3 of species-mixing experiment using SPATAC; Homo sapiens; Mus musculus; ATAC-seq
Nuclei of Hepa and K562 cell lines were isolated according to the Omni-ATAC protocol. In brief, 10 million cells were pelleted by centrifugation at 500 x g for 5 min at room temperature. Supernatant was discarded and the cell pellet was lysed with the Omni-ATAC lysis buffer containing 0.1% NP-40, 0.1% Tween-20, 0.01% digitonin (G9441, Promega), 10 mM NaCl, 3 mM MgCl2 and 10 mM Tris-HCl pH 7.4 for 3 min on ice. Then, 10ml NWB (10 mM Tris-HCl pH 7.4, 10 mM NaCl (AM9759, Thermo), 3 mM MgCl2 (AM9530G, Thermo), 1% BSA (130-091-376, Miltenyi Biotec) and 0.1% Tween-20 (11332465001, Sigma))  were added to lysed cells. Nuclei were spun down for 5 min at 500 x g at 4°C, and resuspended in 10ml NWB. The following steps were processed according to the fixation protocol of embryo nuclei above. Nuclei were then counted on a hemocytometer and diluted to 5,000 nuclei per 1 μl using NWB. SPATAC, a customized single-cell ATAC-seq library construction method, see description of our paper for details.For species-mixing experiment, we just used the first eight indexed Tn5 transposome. Illumina NovaSeq 6000 System and Hiseq X Ten System