Nano-DMS-MaP of hc16: IVT 85mM DMS rep1
in vitro transcription of hc16 (with 3' M13 rev adapter sequence), in vitro folding, DMS probing, RNA purification by sodium acetate-ethanol precipitation, reverse transcription with primer M13_pA_re, PCR with primers PCR_hc16_fw and M13_pA_re, and sequencing with native barcoding kit Nanopore SQK-NBD112.96