GSM7029505: 22Rv1_CRISPRi_clone3_Tigger3a_Cas9_rep1; Homo sapiens; OTHER
For ChIP-seq cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using the Diagenode Bioruptor 300. For Cut&Run cells were incubated with concanavalinA beads, followed by incubation with antibody with digitonin-containing buffer. After washing, samples were incubated with G-protein coupled with MNase and exposed DNA was digested by activating MNase with CaCl2 for 30 min in ice bath. ChIP-seq and Cut&Run libraries were prepared using the Takara Thruplex Library Preparation Kit. 10 ul of  eluted DNA was used as starting material for input and/or IP samples. Post amplification libraries were size selected at 250-450bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.