GSM6797971: Abin1Q478H/Q478H  bone marrow lineage-negative cells replicate 2; Mus musculus; RNA-Seq
Lineage-negative cells cells from three WT and three  Abin1Q478H/Q478H mutation mice were purified by flow cytometry. Lineage-negative cells were then stained with Antibody Biotin CD3, Biotin CD45R,Biotin CD4, Biotin CD5 , Biotin CD8a Biotin ,Biotin CD19,Biotin CD11b,Biotin Ly-6G, Biotin TER-119, PE-Cy7 Streptavidin, and selected by beckman moflo Astrios EQ . Total RNAs from sorted lineage-negative bone marrow cells of WT and AbinQ478H/Q478H  mice at 5 months old were extracted with Trizol (9108, Takara). Each group had three independent samples. RNA samples were quantified using a Qubit 3.0 Fluorometer (Life Technologies, CA, USA), and RNA integrity was assessed using a 4200 TapeStation (Agilent Technologies, CA, USA). RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ II mRNA Library Prep Kit following manufacturer's protocols.