GSM7949449: ASPC1-DMSO-p11; Homo sapiens; OTHER
Genomic DNA exptration was carried out using the Wizard Genomic DNA purification kit (Promega, A1120)  according to the manufacturer's protocol A two step PCR was performed to enrich guide RNA regions in the genome and to amplify the guide RNAs with Illumina TruSeq adapters with i5 and i7 indices.