RNA-seq of solanum tuberosum: leaves sprouted from tubers
Tissue samples were excised from potato sprouts from tubers and leaves. Total RNA was extracted with the TRIzol reagent ((Invitrogen, Thermo Fisher Scientific, USA) as recommended by the manufacturer. The samples were treated with DNase I (DNase I, RNase-free, HC (50 U/L) - Thermo #EN0523) and rerecipitated with trizol-chloroform. Then, the quantity and  quality  of the RNA preparations were assessed using NanoDrop ND-1000 (Nanodrop Technologies, Wilmington, DE, USA), and agarose gel electrophoresis, respectively. The high-quality RNA was subsequently used for RNA-seq. 1 sample is an average sample from 10-25 plants.