GSM7061848: MIA PaCa-2, hypoxia, CTRL, 3; Homo sapiens; RNA-Seq
Total RNA was extracted using the NucleoSpin RNA extraction kit (Macherey-Nagel). RNA quality was assessed by bioanalysis (Agilent) and samples with RNA integrity number > 7 were included for RNA sequencing using > 200 ng of total RNA. RNA libraries for RNA-seq were prepared using KAPA RNA HyperPrep Kit with RiboErase (Roche) following manufacturer's protocols.