GSM8150407: scRNA-seq_αLP_hashtag_v2; Mus musculus; RNA-Seq
Bone marrow cells were harvested from the cut-off of both ends of the femur, tibia and pelvis, humerus, and scapula of mice using a centrifuge. Lineage-positive cells were depleted using a MOJO Hematopoietic Isolation Kit. αLP were sorted using FACSAriaII (v1) and BD Melody (v2). Hashtag laveled sorted cells were combined, trapped, and reverse-transcribed using BD Rhapsody (BD) according to the manufacturer's instructions. Single-cell RNA sequencing was performed using the platform of TAS-Seq.