GSM7051832: scRNAseq, CGE E14.5 WT dissection; Mus musculus; RNA-Seq
E14.5 C57BL/6 WT brains were extracted in ice-cold HBSS and sectioned. Caudal Ganglionic Eminence microdissected tissue was then incubated in 0.05% Trypsin at 37°C for 5 minutes. After digestion, FBS was added and cells were mechanically dissociated via pipetting and centrifuged 5 minutes at 300g. Cell pellet was then suspended in 1ml HBSS and filtered using a 70um strainer. Cell suspension was loaded into a 10X Chromium controller and processed with the Single Cell 3' v2 reagent kit. Single cel libraries were then sequenced using a HiSeq 4000 instrument.