GSM8247989: Humanised mouse spleen MEKi experiment, GEX; Homo sapiens; OTHER
CD45+ cells were sorted from splenocytes. Single cells were encapsulated in  Gel-Beads-in emulsion (GEMs) using the 10X Controller with a Chromium Single Cell 5' GEM Library and Gel Bead kit (Garvan Weizmann Centre for Cellular Genomics). The cell hashing protocol described by Stoeckius et al  (Stoeckius et al.,2017)  was used to generate a hashtag oligo (HTO) library, adapted with a 5'v2 immune profiling protocol with TCR. https://citeseq.files.wordpress.com/2019/02/cite-seq_and_hashing_protocol_190213.pdf CITE-seq VdJ Immunoprofiling