GSM7911813: WT_3; Homo sapiens; RNA-Seq
Total RNA was extracted from three replicates of native human islets and reformed islets from one donor, using the TRIzol method. 1.2 ug of total RNA was used for the construction of the sequencing libraries. Library preparation and RNA sequencing were performed by Single-Cell Discoveries using the QuantSeq 3 kit (Lexogen) and sequenced on an Illumina NextSeq 500 with a sequencing depth of 10 million reads per sample.