GSM6743920: CCl4_library2, scRNA-seq; Mus musculus; RNA-Seq
The colon tissues were enzymatically digested into single-cell suspensions using Multi Tissue Dissociation Kits (Miltenyi Biotec) The single-cell suspensions were converted to barcoded scRNA-seq libraries through steps including droplet encapsulation, emulsion breakage, mRNA captured bead collection, reverse transcription, cDNA amplification, and purification.