GSM8324418: HiChIP_EC-23_H3K27ac_18351X4_Seq_combined_E2_rep2; Homo sapiens; OTHER
Chromatin was crosslinked with 1% formaldehyde in growth media and isolated using Hi-C lysis buffer supplemented with protease inhibitor. Antibody (Abcam, ab4729) against H3K27ac was used to pull down crosslinked chromatin associated with H3K27ac overnight at 4C. Hi-ChIP as previously described by M. R. Mumbach et al.,.Nat Methods 13, 919-922 (2016).