Ganaspis brasiliensis: G1-Tokyo
DNA was extracted and libraries were prepared and sequenced at the DNA Sequencing & Genotyping Center, Delaware Biotechnology Institute, University of Delaware. Briefly, genomic DNA was extracted from an individual male from each population by cryo-pulverizing it in a 1.5 ml tube. After pulverizing, DNA was separated from other material with HMW MagAttract kits (Qiagen, Germantown, Maryland, USA). Lysis was done at 56C for 30 minutes, but the shaking steps were replaced by 15 minutes of rotation to reduce DNA shearing. Extracted DNA was quantified with a Qubit fluorometer (Invitrogen, Waltham, Massachusetts, USA), and DNA length distributions were determined on a Femto Pulse electrophoresis system (Agilent Technologies, Santa Clare, California, USA). PacBio HiFi libraries were prepared from the extracted DNA with low input and ultra-low input kits (Pacific Biosciences, California, USA). The resultant libraries were sequenced on a PacBio Sequel IIe.