GSM6645425: Sample pool 3 of single strain transplanted or co-transplanted MDST tumors treated with or without olaparib for 20 days; Homo sapiens; RNA-Seq
Tumor was harvested from FVB mouse mamary pad. Tumor tissue was dissociated with gentleMACS tissue dissociation kits (SUM149 xenograft tumors were disaggregated by gentleMACS kits (Miltenyi Biotec, #130-096-730), and incubated in cell multiplexing oligos from 10x Genomics (3' CellPlex Kit Set A 1000261) following the manufactural instructions. Live cells were isolated by EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899). Single cell suspensions were processed according to 10x Genomics scRNAseq sample preparation protocol (Chromium Single Cell 3' v3.1 Reagent Kit, 10xGenomics). 5000 cells were targeted for each sample.) RNA libraries were prepared for sequensing using standard library construction protocol by 10x Genomics