GSM8425525: P25 neocortex, Chd8 het male, bulk RNA-seq, rep3; Mus musculus; RNA-Seq
For embryonic samples, the dorsal telencephalon was dissected from six litter- and sex-matched pairs of wild type and Chd8+/- embryos at two time points: embryonic day (E) 12.5 and E17.5. The medial dorsal telencephalon was removed to avoid capturing the hippocampal primordium and flash-frozen in liquid nitrogen until RNA extraction. For postnatal day (P) 25 samples, the cerebral cortex was dissected from six litter- and sex-matched pairs wild type and Chd8+/- mice, followed by removal of the medial cortex and flash-freezing in liquid nitrogen until RNA extraction. RNA was extracted using either the QIAGEN miRNeasy Micro Kit with on-column DNase digestion (E12.5 samples) or the QIAGEN miRNeasy Mini Kit with on-column DNase digestion (E17.5 and P25 samples). PolyA-enriched RNA libraries were generated using the Roche Kapa mRNA Hyper Prep Kit, then sequenced (2 x 100bp) using standard Illumina protocols on an Illumina NovaSeq 6000.