GSM7102083: lineage-ckit-Sca1-_miR-221/222-proficient; Mus musculus; RNA-Seq
Single cell BM suspensions of unperturbed miR‑221/222 proficient and deficient mice were stained for FACS and MPP(3-4), CLP, lin-c-kit+Sca1- and lin-c-kit-Sca1- cells were sorted. MPPs were sorted as pools of MPP3+MPP4 populations as ineage-ckit+Sca1+CD150-CD48+ population. CLP was sorted as lineage-ckitloSca1loFlk2+IL7R+ population. Cells from lineage- population were sorted as ckit+Sca1- and ckit-Sca1- populations. Single cell BM suspensions of unperturbed miR‑221/222 proficient and deficient mice were stained for FACS and MPP(3-4), CLP, lin-c-kit+Sca1- and lin-c-kit-Sca1- cells were applied to the 10x Genomics workflow for cell capturing and generation of scRNA gene expression (GEX) library using the Chromium Single Cell 3′ Library & Gel Bead Kit. Chromium Single Cell 3′ Library & Gel Bead Kit from 10x Genomics was used.