GSM7850429: ce_mRNA_ebax_TC_mut_19h; Caenorhabditis elegans; RNA-Seq
We harvested worms from 18h to 31h in hourly interval. Worms were collected from plates and washed 3 times and resuspended in Tri Reagent (MRC, TR118) and RNA was extracted using  Direct-zol™ RNA MicroPrep kit (Zymo Research; R2062) as per manufacturere's instructions. Total RNA was treated with DNAse on column Sequencing libraries were generated using Illumina Stranded mRNA Prep Kit according to the manufacturer's instructions. Subsequently, library was sequenced on NovaSeq6000, as 56 cycles paired-end reads.