GSM7061982: siTFAP2A_TCDD_rep1; Homo sapiens; RNA-Seq
Total RNA was isolated using the Favorprep total tissue RNA kit (Favorgen Biotech, Taiwan), according to the manufacturer's protocol. 5 ng total cDNA as measured by Qubit (Invitrogen) was used for RNA-seq library preparation. Adaptors were ligated to the DNA fragments, followed by a pre-PCR of 4 cycles, size selection (∼300 bp), and subsequently 11 cycles of PCR amplification. Cluster generation and sequencing (50 bp) was performed with the hiSeq Solexa Genome analyzer according to standard Illumina protocols.