GSM7912819: WT CAR T cells, CAR product, biological replicate 3; Homo sapiens; RNA-Seq
Total RNA was extracted from samples using column-based purification, followed by polyA selection to enrich for mRNA Following purification, enriched mRNA was fragmented, followed by synthesis of first strand cDNA using reverse transcriptase and random primers. Second strand synthesis performed using SMM with DNA polymerase I and Rnase H.