GSM7102712: Kidney,DD8-4,WT; Mus musculus; RNA-Seq
We extracted total RNA from tissue samples using Trizol (Invitrogen). After separating the RNA, DNA, and protein layers with chloroform, we precipitated the RNA using a standard isopropanol/ethanol procedure. The resulting RNA pellet was washed, and then resuspended in RNase-free water. We assessed the quantity and quality of the total RNA using the Qubit RNA HS Assay (Life Technologies) and RNA 6000 Nano Assay on a Bioanalyzer 2100 (Agilent). For RNA-seq library construction, we followed the Illumina TruSeq Total RNA stranded protocol.