GSM7990036: FMT CZA + DAV132 + PD-1_T026; Mus musculus; RNA-Seq
Total RNA was extracted from cells by using Monarch Total RNA Miniorep Kit according to the manufacturer's instructions. 300 ng of total RNA were ribodepleted with the Ribocop rRNA depletion kit. Then, libraries were prepared thanks to NEBNext Ultra II Directional RNA library prep kit for Illumina according to manufacturer's instructions.