GSM7061721: AP2-FG_ChIP_PFG(-)_2_IP; Plasmodium berghei; ChIP-Seq
Blood was filtered with Plasmodipur to remove lymphocytes and fixed with 1% formaldehyde for 60 min at 30C with shaking. After erythrocyte lysis in 0.85% NH4Cl, residual cells were subjected to ChIP-seq. Cells were sonicated with Bioruptor in the following condition: total of 20 times at 30 s in middle power in the cell lysis solution containing 1% sodium dodecyl sulfate. The supernatant was subjected to immunoprecipitation with anti-GFP antibodies using Dynabeads protein A. Libraries were constructed using KAPA Hyperprep kit (KAPA Biosystems), and sequencing was performed with Illumina NEXTseq sequence (Single read - illumina NextSeq 500),