GSM7852762: TM+CO rep1; Mus musculus; OTHER
1x10^5 cells were harvested, counted and centrifuged for 5 min at 500g at room temperature. Cell activity was detected with LUNA-FLTM and counted. Cells are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed on the Agilent Bioanalyzer 2100 system. The library preparations were sequenced on Illumina Novaseq platform and 150bp paired-end reads were generated.