GSM8425572: Epidermis Replicate 5; Anas platyrhynchos; RNA-Seq
RNA was isolated using the Quick-RNA Microprep Kit (Zymo) per the manufacturer's instructions. RNA concentrations of 113–782  pg/µl and RIN values in the range of 7.1–9.5 were acquired from inner cores, assessed via a 2100 Bioanalyzer (Agilent). RNA from a total of 6-7 inner cores was collected from 5 independent embryos. RNA was also isolated from the epidermis of 6 separate bill-skin preparations as a control. Libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England Biolabs)