GSM6797953: EPB41L5 KO-1 repl.-2; Homo sapiens; RNA-Seq
Cell were washed in PBS, scraped into PBS and pelleted by centrifugation. RNA was isolated from cell pellets using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) including gDNA depletion by gDNA binding columns and on column DNase-1 digest according to the supplier instructions. RNA quality number (RQN) was measured as 10.0 for all samples using an Agilent Fragment Analyzer system. After polyA selection, RNA libraries were prepared for sequencing using the NEBnext ultra II RNA directional kit. Libraries were sequenced on the Illumina NovaSeq 6000 sequencer following the manufacturer's protocols.