GSM8409634: KO ILC2-3; Mus musculus; RNA-Seq
Total RNA from sorted lung ILC2s of R5/+ (WT) and R5/+ Rptorf/f (KO) mice in steady state was extracted by Trizol reagent.  RNA quality was examined with an Agilent Bioanalyzer 2100 (Agilent). The concentration of RNA was from 7ng/ul to 14ng/ul. The SMART-Seq HT Kit (Takara Bio USA, Inc) was used to generate high-quality, full-length cDNA. Libraries were generated using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) following manufacturer's protocol.