GSM7949321: HCT116_WT_WGBS; Homo sapiens; Bisulfite-Seq
Genomic DNA was isolated using the DNeasy Tissue & Blood kit (Qiagen) according to the manufacturer's protocol. The library preparation for WGBS was performed with the tPBAT protocol described previously (Miura F, Nucleic Acids Res, 2012; Miura F, Nucleic Acids Res, 2019). One hundred nanograms of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA (Promega) was used for the library preparation.