GSM7432477: B2x2_D_Nira_r3; Homo sapiens; OTHER
Phenol-chloroform extraction for genomic DNA Using primers containing NGS barcode attachment sites (GCTCTTCCGATCT), the mutagenized regio was amplified with GXL DNA polymerase. Amplicons were then purified using an automated SPRI beads purification protocol at the UC Berkeley DNA Sequencing Facility, i5/i7 barcoded and pooled.