Whole genome sequencing of functionally screened E. coli library
Metagenomic DNA from fecal samples of ~20 cows were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into the pZE21 expression vector and transformed into E. coli. These E. coli transformant libraries were then screened against an antibiotic at a selective concentration. Resistant clones were collected from solid media; the plasmids were isolated and  sequenced using Nextera protocol on Illumina NOVAseq 6000 platform.