GSM7597432: rgl2_1dL_Rep1; Aethionema arabicum; RNA-Seq
Intact, non-germinating seeds were collected into liquid nitrogen. RNA was extracted as described at Chang et. al. 1993 (http://dx.doi.org/10.1007/BF02670468) followed by additional purification using columns (QIAGEN RNeasy Kit, Cat. No. 74104). Libraries were constructed from 500 ng RNA using NEB mRNA seq library preparation Kit including  Poly(A) RNA Selection Kit.