RNA-Seq of mus musculus: old adult male spinal cord
Total RNA was rRNA depleted, fragmented and first and second (dUTP) cDNA strands synthesised. Adaptors were ligated and the first strand underwent 13 cycles of PCR amplification. cDNA was sequenced using TruSeq PE Cluster Kit v3 reagents and the Illumina HiSeq 2500 system