H1_PCR4
The V1-V9 region of the 16S rRNA gene was sequenced using the ONT MinION device and the 16S Barcoding Kit 1-24 (SQK-16S024) version 16S_9086_v1_revR_14Aug2019 according to the manufacturers protocol with the previously mentioned modifications of input DNA and PCR conditions. In both cases (fish and mock community samples), after PCR, amplicons were quantified using PicoGreen (Thermo Fisher Scientific) and 100 fmol were loaded into the MinION device. Libraries were sequenced using an R9.4/FLO-MIN106 flow cell and demultiplexed  using  MinKNOW v21.11.17.