DNA-Seq of P. gingivalis: tissue of patient with esophageal cancer
Take 100ng of whole gene gDNA and randomly break it into fragments of less than 500bp using an ultrasonic fragmentation instrument (Covaris S220). Then, perform end repair (including 5 'end phosphorylation and 3' end addition of 'A') using End Prep Enzyme Mix, add sequencing connectors at both ends, purify the target fragment using magnetic beads, and amplify using P5 and P7 primers. Use Agilent Technologies, Palo Alto, CA, USA to detect library quality, and Qubit 3.0 to detect library concentration. Use qualified libraries for cluster preparation and sequencing