GSM7789715: luminal differentiated  type rep4; Mus musculus; RNA-Seq
mammary gland tissue was dissociated using gentle collagenase/hyaluronidase (Stemcell), mammary epithelial cells were further isolated using trypsin (Gibco) and dispase (Stemcell) treatments. Luminal progenitor and mature cells were sorted via FACS based on EpCAM, CD49f, cKit and CD14 surface markers. RNA was extracted using Rneasy Plus Micro kit (Qiagen). 500 pg of RNA was used for cDNA amplification using NEB Kit #E6421 "NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification" RNA-Seq libraries were generated using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770/E7775) according to the manufacturer's protocol. 8 cycles were used to amplify the libraries.