WT3D
WT mice were anesthetized, and fur removed to use ophthalmic scissors that create regular rounded wounds 1 cm in diameter. The wound was covered with woundplast on the day of surgery. Wound closure was documented by imaging beginning on day 0 and analyzed on day 3. Mice were sacrificed by excessive anesthesia and fur removed. The skin wound granulation tissue was sheared, weighted and rinsed in culture medium. Using Mouse Epidermis Dissociation Kit and according its instructions to isolate skin wound cells.And macrophages were sorted by flow cytometry.