GSM6744152: CMO_iHCF_72h_Control_lot1; Homo sapiens; RNA-Seq
After trypsinization, cell suspensions were labeled with barcoded lipids from the 3' CellPlex Kit Set A (10x Genomics PN:1000261) following manufacturer's instructions (Protocol CG000391). After labeling, technical replicates were pooled and loaded onto a Chromium Controller (10x Genomics) at a concentration to capture 6-10,000 cells. Single-cell libraries were prepared using the Single Cell 3' Kit v3.1 (10x Genomics PN:1000268) according to manufacturer's instructions (User Guide CG000388). Gene expression and cellplex libraries were indexed with Dual Index Kit TT Set A and NN Set A indexes (PN:1000215, 3000482) respectively.