GSM7597586: L17_1; Saccharomyces cerevisiae; RNA-Seq
For RNA extraction, library preparation, and sequencing, total RNAs were extracted from four strains using TRIzol Reagent (Invitrogen, cat. NO.15596026); RNA was extracted using DNase I for DNA digestion. RNA quality was determined by examining the A260/A280 ratio with a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific Inc). RNA integrity was confirmed by Qsep100 (BiOptic Inc.)/5300 Fragment Analyzer system (Agilent). Qualified RNAs were quantified using a Qubit3.0 with the Qubit TM RNA Broad Range Assay kit (Life Technologies, Q10210). Two micrograms of total RNA were used for stranded RNA sequencing library preparation using the KC-Digital TM Stranded mRNA Library Prep Kit (Catalog No. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instructions.