RNA-Seq of Rubus idaeus: leaf sample of isolate V2
Double-stranded RNA isolated from sample V2 and treated with DNase on a column was checked by an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). An RNA library was prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7300S) with Dual Index Primers Set 1(#E7600S), and sequenced on the NovaSeq 6000 platform (Admera Health Biopharma Services, NY, USA) using a paired-end (2 150) configuration.