GSM8150734: Prss56_E12; Mus musculus; RNA-Seq
E11.5 and 12.5 Egr2Cre/+,Rosa26RTom and Prss56Cre/+,Rosa26RTom embryos were identified on the basis of Tomato expression under fluorescent stereomicroscope (Leica, Nussloch, Germany). Meninge and DRG was dissected and then digested with collagenase/dispase type I (Merck/Roche) for 15 min at 37°C. Digestion was stopped by addition of 0.1 ml of fetal calf serum. Samples were slowly mechanically dissociated and the cell suspension was filtered. Dissociated cells were then resuspended in PBS, 1% BSA, and subjected to FACS. Tomato-positive cells were isolated, while dead cells and doublets were excluded by gating on a forward-scatter and side-scatter area versus width. Log RFP fluorescence was acquired through a 530/30 nm band pass. Internal Tomato-negative cells served as negative controls for FACS gating. Tomato-positive cells were sorted directly into PBS, 0.04% BSA for scRNA-seq experiments. Libraries were prepared according to 10x 3'expression V2 kit  & Illumina protocols