RNA-seq for circRNA of the septic mice lung tissue
The sequence data were filtered with SOAPnuke (v1.5.2 https://github.com/BGI-flexlab/SOAPnuke). The cleaned data were stored in a FASTQ format and subsequently imported into the Dr. Tom bioinformatics analysis system (https://biosys.bgi.com/) for further analysis. HISAT2 (v2.0.4) was used to map the clean data to a reference genome. The Mus musculus genome (GCF_000001635.26_GRCm38.p6) was selected as the reference genome. Bowtie2 (version 2.2.5) was applied to align the clean data to the gene set, a database by BGI, in which known and novel coding and noncoding transcripts were included.