GSM6744187: IP_ZBP1WT_TRF2_1; Homo sapiens; RIP-Seq
Total RNA was isolated using TRIzol (Invitrogen #15596018) and purified with RNAeasy Mini Kit (Qiagen #74106). The quality of the isolated total RNA was assessed using Agilent TapeStation 4200. Sequencing libraries were prepared with immunoprecipitated RNA using the TruSeq stranded total RNA sample preparation kit according to the manufacturer's protocol (Illumina). RNA-seq libraries were multiplexed, normalized, and pooled for sequencing. The libraries were sequenced on the MiniSeq system (Illumina) at paired-end read 37bp.