GSM7508514: lipl-4Tg-H3K79me3-vs-H3-rep2; Caenorhabditis elegans; ChIP-Seq
Age synchronized worms at day-1 adulthood were used for sample preparation. Samples were fixed in 1% formaldehyde/PBS solution for 10 min. Crosslinking was quenched by adding glycine solution to a final concentration of 125 mM. Chromatin was sonicated at 4 °C using a Branson 250 sonicator. The ChIP-seq formulated antibodies were anti-histone H3 (CST #4620), H3K79me2 (CST #5427), H3K79me3 (Abcam ab2621). Sequencing libraries were constructed by NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads kit (NEB #E7103S) according to manufacturer's instructions. Liberaries were pooled together and sequenced on the Illumina NextSeq 500 system.