GSM6647828: HC PBMC; Homo sapiens; RNA-Seq
PBMC were isolated with the use of Ficoll density gradient centrifugation PBMC suspensions (300-600/μl) were mixed with 10x Barcoded Gel Beads and formed Gel Bead-In-EMulsions (GEMs) in the GemCode instrument, where cell lysis and barcoded reverse transcription of RNA occurred, followed by amplification, enzymatic fragmentation, 50 adaptor, and sample index attachment. The libraries were constructed using Chromium Single Cell 30 Reagent Kits (v2): Single Cell 30 Library & Gel Bead Kit v2 (PN-120237), Single Cell 30 Chip Kit v2 (PN-120236), and i7 Multiplex Kit (PN-120262) (10x Genomics).