GSM8325864: ST2_Arid5a_2_IL17; Mus musculus; RIP-Seq
Cells were trypsinized and crosslinked by 1% formaldehyde (Thermo Scientific). Extracts were isolated with cell lysis buffer II (Invitrogen) with RNase Inhibitor (40 U/μl; Thermo Scientific). Buffers included protease inhibitor cocktail (Sigma-Aldrich). Lysates were immunoprecipitated with Abs against Arid5a or mouse IgG2b. Beads were washed with lysis buffer and digested with DNase I (Roche Applied Science) and protease K (Sigma-Aldrich). Total RNA was extracted with acid phenol. SMART-Seq Stranded kit from Takara