NextSeq 500 paired end sequencing; ChIP-seq for RUNX1 and ETV6-RUNX1 in an induced pluripotent stem cell model of ETV6-RUNX1 pre-leukaemia
ChIP-seq for RUNX1 and ETV6-RUNX1 in an induced pluripotent stem cell model of ETV6-RUNX1 pre-leukaemia
Induced pluripotent stem cells were subjected to B-cell differentiation as described in Richardson et al, 2021 https://doi.org/10.1016/j.xpro.2021.100420. Cells were harvested and subjected to CD45 MACS enrichment according to manufacturer's instructions (Miltenyi Biotec, 130-045-801). https://doi.org/10.1016/j.xpro.2021.100420 Cells were crosslinked with 1% formaldehyde and sonicated to yield chromatin of 100â€“500 bp. ChIP was performed by as previously described (May et al., 2013) using antibodies: RUNX1 (Abcam, ab23980), V5 (Abcam, ab9116). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645)