Ribo-seq-Psph-WT-1
Overnight cultures of strains were transferred into fresh KB medium and culture for 6hours, and chloramphenicol was added before centrifugation. The pellet was resuspended in lysis buffer and fast-frozen in liquid nitrogen. MNase then digested the supernatant. Sephacryl S400 MicroSpin columns were used to purify the MNase-digested products. The sRNA was separated using a Zymo RNA kit, and the rRNA was removed. The final library was constructed using the NEBNext Small RNA Library Prep Set.