GSM7432857: HepG2 cells, PABPC1-RIP, rep1; Homo sapiens; RIP-Seq
HepG2 cell pellet was lysed and RNA was immunoprecipitated with pre-prepared antibody conjugated protein A/G magnetic beads. After washing, RNA was extracted by TRI Reagent and used for further sequencing. Libraries were prepared using EpiTM mini longRNA-seq kit following manufacturer's protocols. SMART-seq use random primers