GSM7147274: MPC3.shRNF185.3_rep4; Mus musculus; RNA-Seq
RNA from samples were purified using a GenElute total RNA purification kit (Sigma-Aldrich). PolyA RNA was isolated using NEBNext® Poly(A) mRNA Magnetic Isolation Module, and bar-coded libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and sequenced from a single-end (1X75) on an Illumina NextSeq 500 system using the High output V2 kit (Illumina Inc., San Diego, CA).