GSM8247260: STAR-3 T cells,CD8 subset, cultured with KMS26-MUT, experiment 1, replicate 1; Homo sapiens; RNA-Seq
Total RNA was isolated with the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer's instructions and quantified by RNA ScreenTape (Agilent, 5067-5576). The SMART-Seq mRNA LP (with UMIs) kit (Takara, 634765) and the Unique Dual Index Kit (1–96) (Takara, 634752) were used to generate cDNA and prepare sequencing libraries from two independent batches of T cells, with 3 independent cDNA libraries per condition. Libraries were sequenced on a NovaSeq 6000 (Illumina) using the 100 cycle single-end configuration at approximately 25 million reads per technical replicate.