GSM7789694: basal wild type rep4; Mus musculus; RNA-Seq
mammary gland tissue was dissociated using gentle collagenase/hyaluronidase (Stemcell), mammary epithelial cells were further isolated using 0.25% trypsin (Gibco) and 5 mg/mL dispase (Merck). Wild type luminal and basal cells were sorted via FACS based on  EpCAM and CD49f surface markers (antibodies 12-9326-42 from eBioscience and 313631 from Biolegend). RNA was extracted using Rneasy  kit (Qiagen).  1 ug of RNA was used for library prep. RNA-Seq libraries were generated using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770/E7775) according to the manufacturer's protocol.