GSM8247386: WT, ZT6, rep5; Physcomitrium patens; RNA-Seq
Frozen samples were powdered using a cold mortar and pestle, in presence of liquid nitrogen. Approximately 150 mg of powder were used for RNA extraction using the RNeasy Plant Mini Kit, ref. 74904 (Qiagen). RNA was used to build cDNA libraries using the kit QuantSeq 3' mRNA-Seq Library Prep Kit FWD (Lexogen), which exploits oligo-d(T) primers therefore enriching the cDNA library in cDNA representative of mRNA, but not ribosomal RNA or other types of RNA. Libraries were then sequenced with a depth of 5 million of reads using a NextSeq 500 System (Illumina).