GSM7818268: Bulk RNA-seq of sorted CD8 T cells from CSF of another person living with CRPS; Homo sapiens; RNA-Seq
For bulk sequencing,lysed cells were directly amplified into double stranded cDNA using the SMARTer protocol as described elsewhere (Picelli et al., 2014). All three cell types (CD4+ T cells, CD8+ T cells and monocytes) derived from one individual would be amplified at once. For single cell sequencing, we used a 10X Chromium Platform following manufacturer's protocols. For bulk sequencing, library preparation from cDNA and sequencing were performed by an external company (Genewiz). For CITE-sequencing, the King's BRC Genomics facility followed the workflow recommended by 10X.