Illumina HiSeq of Attagenus unicolor japonicus: adult
At least one g of purified DNA was used to construct the TruSeq DNA library (insert size = 400 bp) according to standard protocols. The library was sequenced using the Illumina HiSeq 4000 platform (Nanjing Personal Gene Technology Co., Ltd., China) with paired-end reads of 2  150 bp. The unpaired, short, and low-quality raw reads were filtered to obtain clean reads.