GSM6645212: Zminus6; Danio rerio; RNA-Seq
Total RNA was isolated from fish liver using Trizol lysis reagent (Sigma Aldrich) followed by isopropanol precipitation and the RNA pellet washed with 70% (v/v) ethanol. PolyA RNA seq libraries prepared using Ilumina TruSeq Stranded mRNA Sample prep kit following manufacturer's protocols.