GSM7102107: HPC7, ATAC-seq, rep1; Mus musculus; ATAC-seq
We lysed pelleted cells with lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% NP-40, 0.01% Digitonin (Invitrogen, cat# BN2006)) for 3 minutes on ice, then we washed nuclei with wash buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 1% BSA, 0.1% Tween-20). ATAC-seq libraries were prepared according to Buenrostro et al. 2013 (PMID:24097267) with some modifications.