sRNAseq sperm non-absorbable antibiotic 2
At 5 weeks of age, specific pathogen free (SPF) male C57BL/6J inbred mice were transferred to a conventional colony facility. The male mice were divided into separate cages (one mouse/cage) and randomly assigned to two groups: (i) Control group, which received regular diet and sterile filtered (0.2mm) water for six successive weeks (abbreviated as CON mice); (ii) Treated group, which received regular diet and sterile filtered (0.2mm) water supplemented with a cocktail of FDA-approved non-absorbable antibiotics (neomycin 2.5 mg/ml, bacitracin 2.5 mg/ml and pimaricin 1.25mg/ml, abbreviated as nABX) for six successive weeks (6wk nABX). The water bottle and nABX cocktail were replaced freshly every 5 days.