GSM6647479: WT alveolar macrophage, replicate 1; Mus musculus; RNA-Seq
PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module. Barcoded libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (NEB, Ipswich MA). Libraries were pooled and sequenced single-end (1X75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).