GSM6705132: HSC,WT,rep3,RNA-seq; Mus musculus; RNA-Seq
Lysates were clarified from FACS-sorted  hematopoietic stem cells. 200 HSCs (Lineage- Sca-1+ c-kit+ CD34- CD150+) from WT or Mcph1-/- mice were sorted into lysis buffer directly. RNA-seq:The transcriptome libraries were prepared using Smart-seq2 method. Then, Agilent Bioanalyzer 2100 system (Agilent Technologies) was used to assess the insertion size, and HiSeq PE Cluster Kit v4-cBot-HS (Illumina) was used to perform the clustering of the index-coded samples according the manufacturer's instructions. Finally, the libraries were sequenced using an Illumina Hiseq platform with 150-bp paired-end.