GSM8246922: Human aortic endothelial cells, 48h UF, rep1; Homo sapiens; RNA-Seq
After 48 h flow application, cells were washed with 1X PBS (Gibco #14190-144) and then lysed using RLT lysis buffer (QIAGEN #79216) plus 1% 2-Mercaptoethanol (Sigma Aldrich #M6250). For each condition, four µ-Slides were combined as one flow-exposed sample for a total of 12 slides for n = 3 samples. Libraries for RNA sequencing were prepared using KAPA Stranded mRNA-Seq Kit (KAPA Biosystems #KK8421).