GSM6645411: Luscinia megarhynchos LM30, DNA depleted mRNA from ovaries; Luscinia megarhynchos; RNA-Seq
Total RNA using TRIzol reagent (ThermoFisher Scientific). 9 μl of the total RNA was treated with TURBO DNase (Ambion) to remove genomic DNA followed by a column clean up using RNA Clean & Concentrator kit (Zymo Research) mRNA-seq libraries were constructed with NEB Ultra II stranded mRNA kit (New England Biolabs) including Oligo dT magnetic beads for mRNA enrichment. The libraries were prepared on Biomek i7 Automated Workstation (Beckman Coulter) and sequenced using HiSeq 2500 (Illumina) in 150 bp single-end mode.