GSM7061747: Gametocyte, gSNF2 KO-1; Plasmodium berghei; RNA-Seq
Gametocytes enriched blood were subjected to erythrocyte lysis in 0.84% NH4Cl. Residual cells were lysed in and total RNA was extracted by Isogen 2 (Nippon gene) according to manufacturer protocols. Libraries were prepared using the RNA Hyperprep kit (KAPA Biosystems), and sequencing was performed with an Illumina NEXTseq sequencer.