GSM7029126: miRNA-seq_Plasma exosome Control3; Homo sapiens; miRNA-Seq
Total RNA was extracted from  exosomes using the  Trizol reagent (Thermo Fisher Scientific, Massachusetts, USA), according to the manufacturer's protocol. Subsequently, RNA concentration and purity were detected using nanodrop 2000 apparatus (Thermo Fisher Scientific, Massachusetts, USA). RNA libraries for RNA-seq were prepared using RiboZero Magnetic Gold Kit following manufacturer's protocols.