No stim control for PSS of oligodendrocyte-lineage cell posterior experiments
A photocaged ATAC-seq library was constructed within fixed mouse brain sections by treatment with Tn5 transposase loaded blocked adapters. After particular regions of interest were selected using targeted illumination, the sample was digested and the library was purified. Secondary adapters were ligated to uncaged fragments and used as PCR handles for library amplification. The amplified library was sequenced on an illumina platform.