GSM6705354: hepatocytes_96hpf_wt_expt1_rep4; Danio rerio; RNA-Seq
Hepatocytes were sorted into 75 uL Buffer RLT containing b-mercaptoethanol. Total RNA was extracted using the Qiagen RNA Micro Kit with on-column DNase treatment. The SMART-Seq v4 Ultra Low Input Kit for Sequencing (Clontech) kit was used for cDNA synthesis and amplification. Library prep was performed with the Nextera XT DNA Library Preparation Kit.