Nile tilapia Iso-seq data
PacBio SMRT-seq library construction and full-length transcriptome sequencing was completed by Biomarker Technologies (Beijing, China). Briefly, mRNA was enriched using Oligo d(T) magnetic beads and full-length cDNA was synthesized with SMARTer PCR cDNA Synthesis Kit. The cDNA was amplified through PCR, followed by DNA damage repair, end repair and adapter ligation. The constructed libraries were sequenced using a PacBio Sequel platform.