GSM6743982: HCT116 cells, ov-PBX1-2; Homo sapiens; RNA-Seq
Cells were removed, and RNA was harvested using Trizol reagent. Intact mRNA was isolated from total RNA samples using an Arraystar Seq-StarTM poly(A) mRNA Isolation Kit (Aksomics, Shanghai, China). A KAPA Stranded mRNA-seq Kit (F. Hoffmann-La Roche Ltd, Grenzacherstrasse, Basel, Switzerland) was used to create RNA-seq libraries. The prepared libraries were diluted to a final concentration of 8 pmol/L, and clusters were generated on an Illumina cBot using a HiSeq 3000/4000 PE Cluster Kit (#PE-410-1001, Illumina), followed by sequencing on an Illumina HiSeq 2500.