Genome sequence of Leptospira santarosai strain 2013_VF52
Genomic DNA was successfully extracted from a range of 5 x 10^10 to 2 x 10^11 pelleted L. santarosai cultures using the Dneasy Blood & Tissue Kit by Qiagen. The extracted DNA was subjected to whole-genome sequencing on an Illumina MiSeq platform, utilizing a 250x2-base-pair run. The genomic library was prepared using a Nextera DNA Flex Kit by Illumina.