RNA-Seq of Silurus glanis: eye
Total RNA was extracted from the homogenized frozen tissues (skin, dorsal fin, whole eye and liver; ~ 15-20 mg each) using NucleoSpin RNA extraction kit (MACHEREY-NAGEL, Duren, Germany). The quality of the total RNA sample was evaluated using TapeStation 2200 (Agilent) electrophoresis and sample concentration was measured with Nanodrop ND-2000 (Thermo Scientific). Sequencing libraries were prepared from 500 ng total RNA using the TruSeq stranded mRNA library preparation kit (Cat# 20020595, Illumina Inc.), including polyA selection, and unique dual indexes (at# 20022371, Illumina Inc.) according to the manufacturers protocol (#1000000040498, Illumina Inc.). The library was sequenced using NovaSeq 6000 instrument (2 x 150 bp read length) by the SNP&SEQ Technology Platform (Uppsala, Sweden).