GSM7508704: H2afy CRISPR KO 9464D cells, ATAC-seq, Replicate2; Mus musculus; ATAC-seq
In brief, 50,000 mouse neuroblastoma cells were centrifuged at 500g for 5 min at room temperature for each reaction. The cell pellet was re-suspended in 50 μL lysis buffer. Samples were purified using Qiagen MinElute PCR Purification kit (Qiagen). The transposed DNA was amplified using NEBNext High-Fidelity 2× PCR master mix. Standard ATAC-seq libraries were prepared following the previously established protocol. qPCR was performed to determine the optimal number of cycles for final PCR amplification. The PCR product was purified using Qiagen MinElute PCR Purification kit (Qiagen), followed by a size selection step with SPRI beads with 1:1.2 ratio (Beckman Coulter). Finally, the purified DNA was eluted in 20 μL of Elution Buffer.