RNA-seq of Kappaphycus alvarezii: Brown 3 - Low
Before the onset and after 28 days of the high and low light experiment, samples were collected from apical portion of the thalli, cleaned and stored in frozen tubes. Frozen samples were immediately ground into fine powder using sterile mortar and pestle. About 350 mg of finely ground sample was used for RNA extraction using TRIzol. Precipitation was carried out using isopropanol and ethanol, and the resulting RNA was cleaned up using RNA Clean & Concentrator 5-m kit (Zymo Research). Quality control was performed using gel electrophoresis and Nanodrop spectrophotometry, and the RNA was sent for sequencing using TruSeq mRNA (Illumina) at the Philippine Genome Center.