GSM6798163: w1118 head RNA, control, rep2; Drosophila melanogaster; RNA-Seq
RNA was extracted from 20 fly heads per sample using TRI reagent. Isolated RNA was stored in 80% ethanol. |RNA was quantified using Qubit 3.0. Further RNA integrity was checked on high sensitivity tape (Agilent TapeStation 2200). Samples with RIN number >8.5 were selected for library construction. Three w1118 and three Ssdp[2082-G4] /+ samples were used to prepare 100bp paired sequencing library using the TruSeq Stranded mRNA Library prep kit and >40M read per sample were sequenced on an Illumina NovaSeq6000.