WES of H. sapiens: colorectal cancer tissue
Genomic DNA was isolated from FF tissue of colorectal cancer patients using DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). 100 ng of DNA were fragmented using the SureSelect Enzymatic Fragmentation Kit (Agilent, Santa Clara, CA), libraries were prepared using SureSelect XT HS2 DNA Reagent Kit and SureSelect XT HS Human All Exon V8 (both provided by Agilent) according to manufacturers protocol with the following modification. Samples were pooled for hybridization capture reaction multiplexing 7 samples and using total input 2250ng of DNA per reaction.