GSM7596895: Peromyscus1_CS73_dpS3; Peromyscus maniculatus; RNA-Seq
Retinas were dissected in Ames solution (Sigma-Aldrich; equilibrated with 95% O2/5% CO2 for all use) immediately following enucleation. They were dissociated in papain, followed by FACS sorting. Cells were stained for either THY1/CD90 to enrich for RGCs or for CD73 to deplete photoreceptors. Single cell libraries were generated using the Chromium 3' V3 platform (10X Genomics, Pleasanton, CA) according to the manufacturer's protocol. Briefly, single cells were partitioned into Gel-beads-in-EMulsion (GEMs) where nuclear lysis and barcoded reverse transcription of RNA would take place to yield full-length cDNA; this was followed by amplification, enzymatic fragmentation, and 5' adaptor and sample index attachment to yield the final libraries. Single cell RNA sequecning (10X Genomics)