GSM7051920: scRNAseq, POA E14.5 WT FlashTagged progenitor 87; Mus musculus; RNA-Seq
WT C57BL/6 E14.5 brains  were extracted in ice-cold HBSS 2 hours post FlashTag injection in the lateral ventricles. After sectioning with a Mcllwain tissue chopper, POA was micro-dissected under a stereomicroscope and incubated in 0.05% trypsin at 37°C for 5 minutes. After digestion, FBS was added and cells were mechanically dissociated via pipetting and centrifuged 5 minutes at 300g. Cell pellet was then suspended in 1ml HBSS and filtered using a 70um strainer. FlastTag+ cells were FAC-sorted on a MoFloAstrios device gated to include only top 5% most bright cells. Sorted cells were captured using integrated fluidic circuits and imaged prior to sequencing.