GSM7990771: GTS slow 1; Homo sapiens; miRNA-Seq
Total RNA was isolated by the TRIzol reagent according to the manual (Qiagen, Hilden, Germany). RNA concentrations and purities were measured by the Nanodrop 2000 photo-spectrometer (260 / 280 nm; 260 / 230 nm) and RIN numbers were detemrined by Fragment Analyzer measurememt. 200 ng of total RNA were used for library preparation and microRNA sequencing (microRNAseg). Library preparation (NEXTFLEX Small RNA-Seq Kit v4' (Revvity, Hamburg, Germany)) and microRNAseg (NovaSeq6000 SP 2 x 50 bp) has been performed at the "Institut für Klinische Molekularbiologie" (IKMB) of the University of Kiel. The quality control (QC) and identification of expressed microRNAs has been done by the IKMB.