16S rDNA sequencing of captive Cervus elaphus: feces
Fecal DNA was extracted using the PowerFecal DNA Isolation Kit (MOBIO, USA) in accordance with the manufacturers instructions. The primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) were used to amplify the 16S rRNA gene V3V4 variable region from the bacterial DNA by PCR. The TruSeq DNA PCR-free Sample Preparation Kit (NEB, USA) was used for library construction. After the library was qualified, NovaSeq6000 was used for sequencing.