CART_RNA_DC42_DC44_DC63_DC82_Duplicate1
We utilized hashtag reagents for sample barcoding, enabling the amalgamation of eight samples into a single lane for subsequent demultiplexing during analysis. The combined cells were then subjected to incubation in an antibody cocktail comprising 17 TotalSeq-B anti-human antibodies targeting surface markers. The scRNA-seq libraries were prepared using the Chromium Single-Cell 3 Library and Gel Bead Kit v3.1 (10x Genomics, Cat# PN-1000268).