RNA-seq of C. elegans after heat shock: hlh-17p::HSF-1
RNA isolated from L4 animals heatshocked for 30 min at 34C, RNA captured with oligo-dT beads and first strand synthesis using random priming. Libraries assembled with KAPA mRNA HyperPrep kit and run on Illumina HiSeq4000 using a 2 x 150bp paired end configuration.