Whole genome sequence of SKB/BCL21
Four to five single types of colonies from the culture plate were used as initial starting material and DNA was extracted in 50 L of nuclease free water. The DNA was assessed by agarose gel electrophoresis and fluorometric assay before sequencing. All necessary clean-up steps were carried out using Clean NA magnetic beads for next-generation sequencing. The full genome was assembled with Flye using default settings.