GSM8246840: miRNA_AC16 exosomes, exo-S-Ang1; Homo sapiens; miRNA-Seq
Total RNA was extracted using miRNeasy Micro Kit (Cat #217084, Qiagen), and RNA quality was checked using Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US) according to standard procedures. The exosomes were isolated and total RNA was extracted using exoRNeasy Maxi Kit (#77164, Qiagen). RNA quality was checked using Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US) according to standard procedures. Sequencing libraries were constructed by QIAseq miRNA Library Kit for Illumina (Cat #331505, Qiagen) according to manufacturer's instructions.