RNA-seq of sgLTO1-2
A total of 1 g total RNA per sample was used as input material for the mRNA librarypreparation. Most of Eukaryote 'mRNA have polyA tails, which are enriched by Oligo (dT)magnetic beads. Then, the obtained mRNA was randomly interrupted by bivalent cations, and thefirst strand of cDNA was synthesized in the 1st Strand Enzymes Reverse transcriptase systemusing the segmented mRNA as the template and the random oligonucleotide as the primer, and thesecond strand of cDNA was synthesized using the 2nd Strand Enzymes and dNTPs. The purifieddouble stranded cDNA undergoes end repair, A-tailed addition, and sequencing connections toscreen for cDNA of around 200-300bp. PCR amplification is performed and the PCR product ispurified again, ultimately obtaining a library.And then, library quality was assessed on the Agilent4200 TapeStation.