GSM7101916: Hey2 enhancer-nls-LacZ Tg, scRNAseq; Mus musculus; RNA-Seq
E7.5 embryos were collected in DMEM + 25mM HEPES, and kept on ice during genotyping PCR.  Four Hey2 enhancer-LacZ Tg embryos were selected, and distal part of extraembryonic tissue and posterior half of the embryo were removed.  The sample was treated in 0.25% Trypsin-EDTA with DNase I (50U/ml) (260913-10MU, Millipore) for 10 min at room temperature, and then the reaction was stopped by adding 1/10 volume of fetal bovine serum (FBS).  The dissociated cells were centrifuged at 500g for 5 minutes, and resuspended in PBS + 10% FBS.  The cells were filtered through the cell strainer (70 micrometer, Falcon #352350), and centrifuged at 300g for 5 minutes.  The cells were resuspended in PBS + 10% FBS, and measured cell count and viability. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics).