GSM8403136: MSKB30; Homo sapiens; ATAC-seq
Archival patient PDAC samples from two 50um sections of OCT embedded tumors were broken up using razor blades in petri dish on ice in 1 ml NST-DAPI solution. The crude nuclei preparation was then subjected to FACS on a BD FACSAria Flow Cytometer Cell Sorter to isolate 2N, 4N and aneuploid nuclei.Duplicates of sorted samples were used to confirm KRAS variant allele frequency. Approximately 10,000-50,000 nuclei from either aneuploid or 4N populations were carefully pelleted for ATAC-seq library prep. ATAC libraries were prepared for sequencing using standard Illumina Nextera DNA Library Preparation protocols.