Nano-DMS-MaP of HIV-1 5'-UTR: unspliced mRNA virion 8mM DMS rep2
transfection of plasmid expressing HIV-1 NL4-3 del Gp, 24 h p.tfx. DMS probing, RNA extraction from supernatant, RT with RT-US, PCR with US_fw and US_re, and sequencing with native barcoding kit Nanopore SQK-NBD112.96