GSM7912148: ND-75 strong knockdown heads 4; Drosophila melanogaster; RNA-Seq
20 snap frozen fly heads (10 male and 10 female, 2 days old) were used for each replicate and placed into 100 µL of lysis buffer + β-mercaptoethanol from the Absolutely RNA Microprep kit (Agilent Technologies). Each genotype was prepared in quadruplicate. RNA was extracted from using the Absolutely RNA Microprep kit according to the manufacturer's protocol. Sequencing libraries were generated using NEBNext® Ultra TM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.