GSM6797828: MPE macrohpages, IL-10−/− mice B3; Mus musculus; RNA-Seq
MPE macrophages were isolated using Anti-F4/80 MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). The purity of macrophages was > 90%, which was confirmed by flow cytometry, and cells were cultured in DMEM with 10% FBS for further use. Libraries for sequencing were constructed with NEB Next Ultra RNA Library Prep Kit for Illumina (NEB). Poly(A) tailed mRNA molecules were enriched from 1 μg total RNA with NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit.