Illumina paired-ends reads of Muscicapa striata used for the de novo genome assembly
Short-read DNA sequencing libraries were prepared with the Illumina TruSeq Nano DNA HT Library Prep Kit. Briefly, DNA was fragmented by sonication, size selection (average insert size: 400 bp) was performed using Sample Purification Beads before adaptors ligation. Library quality was assessed on an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR with the Kapa Library Quantification Kit. DNA sequencing (paired-end read length 2x150 bp) was performed on an Illumina HiSeq3000 using the Illumina HiSeq3000 Reagent Kits. Illumina adapters and contaminated reads were removed using Trimmomatic-v0.36.