RNA-Seq of Crassostrea virginica: gonad tissue
Gonad and mantle tissue was processed by Admera Health (South Plainfield, NJ, U.S.A.).  Total RNA was extracted using Qiagen AllPrep DNA/RNA 96 kit (Qiagen, Germantown, Maryland U.S.A), quality was checked using Agilent Tapestation High Sensitivity RNA kit (Agilent, Santa Clara CA, U.S.A). Tagged libraries were constructed with NEB ultra II non-directional kit with Poly A selection (New England Biolabs, Ipswich, MA, U.S.A.) protocol, pooled, and 2x150 paired-end sequenced on an Illumina NovaSeq 6000 instrument with the S4 kit (Illumina, San Diego CA, U.S.A.).