GSM7851391: WT2; Pseudomonas aeruginosa; OTHER
Cycloheximide-treated cells were lysed with lysis buffer and then digested with nuclease. Digested samples were passed through a size exclusion column to isolate single ribosomes. RNA fragments protected by ribosomes were subjected to fragment selection by polyacrylamide gel electrophoresis, followed by removal of rRNA from them. Purified RNA ends were repaired with junctions and converted by reverse transcription to cDNA. cDNA was purified by polyacrylamide gel electrophoresis and amplified by PCR after cyclisation. Ribo-seq