Duplex sequencing of Mus musculus C57BL/6J: 2.5 mo Neil1-/- female liver
DNAs were isolated from 20 mg of homogenized liver tissue using the DNease Blood and Tissue kit (Qiagen, catalog # 69504). Duplex sequencing libraries were prepared from 1 g of enzymatically-fragmented genomic DNA using the TwinStrand DuplexSeq kit and the TwinStrand Mutagenesis Panel (Mouse-50) v1.0 according to the manufacturers protocol. Libraries were sequenced using an Illumina NovaSeq 6000 instrument.