GSM7141591: Brain single cell 528; Mus musculus; RNA-Seq
Fluorescent cells were sorted by FACS from Pdgfra-H2BGFP mice and Tagl-CREERT2;Ai14 mice as previously described (Vanlandewijck et al, 2018). Briefly, brain tissue from both males and females were mechanically and enzymatically digested and vascular fragments isolated using CD31 antibody coated Dynabeads and further digested into cell suspension. Libraries for RNA-seq were prepared by using SMART-seq2 methodology