GSM6744250: HCT116 cells, shMIB1, replicate1; Homo sapiens; RNA-Seq
RNA was extracted using TruSeq Stranded mRNA Sample Prep Kit (Illumina) mRNA molecules were purified and fragmented from 0.1-1 ug of total RNA using oligo (dT) magnetic beads. Fragmented mRNAs were synthesized as single-stranded cDNAs through random hexamer priming. Double-stranded cDNA was prepared by using this cDNA as a template for second strand synthesis. After a sequential process of end repair, A-tailing, and adpter ligation, cDNA libraries were amplifed using PCR.