OPM2:CRISPR/Cas9(Brunello)_CD319(SLAMF7)-FACS_CD319(SLAMF7)-low(5%)
OPM2 cells transduced with the Brunello sgRNA library, stained for CD319(SLAMF7), isolated CD319(SLAMF7)-low-expressing (5%, CD319-lo) cells using FACS, amplified with custom PCR primers containing Illumina sequencing adapters/index