GSM7818049: daf2_1; Caenorhabditis elegans; RNA-Seq
Total RNAs were isolated from frozen worms using RNAiso plus (Takara) and ethanol precipitation. The paired-end mRNA-seq library was prepared and sequenced by a HiSeq 2500 platform at Chunlab (Chunlab, Inc.). A total of three biological repeats of samples was analyzed for all conditions. RNA-seq experiments for two biological repeats were performed first, and the last set was added later to increase the robustness of the data.