GSM7990764: female_thorax_rep3; Anopheles coluzzii; ncRNA-Seq
Total RNA was isolated from heads and thoraxes of adult males and females of the An. coluzzii MOPTI (MRA-763) laboratory colony using TRIzol Reagent (Invitrogen) extraction in three biological replicates.  Thirty individual mosquitoes were used per each replicate. Small RNAs (18-30 nt) were cloned as previously described (Olovnikov et al. 2013). Sequencing was performed in the Duke Center for Genomics and Computational Biology on the Illumina HiSeq4000 to obtain 50-bp single reads. About 18.1 Gb of total data or ~1.5 Gg of data on average for each replicate were obtained. Cloning of small RNA libraries was performed by Illumina TruSeq Small RNA prep kit (Illumina, USA) according to the manufacturer's protocol.