GSM7790914: ADIPO [+MCF7] HG->LG (3); Homo sapiens; RNA-Seq
Total RNA was isolated from cells using TRIzol solution (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions. All RNA samples were quantified by measuring the absorbance at 260nm and 280nm (NanoDrop spectrophotometer, Life Technologies, Carlsbad, CA, USA). The integrity of RNA samples was further analyzed by using the digital electrophoresis system Experion with the “RNA StdSens Kit” (Biorad, Hercules, CA, USA), following the manufacturer's instructions. The run and result analysis were performed by the Experion software. RNA samples with a RNA Quality Indicator (RQI) value ≥ 9 were considered good for the further analysis. Paired-end cDNA libraries were prepared for sequencing on the Illumina Hi-Seq 2500 platform, available at IGA Technology Service (Udine, Italy).