GSM7432466: NIH-3T3 cells, COIL-UV; Mus musculus; OTHER
The UVB lamp model NB-UVB311 (UVB Narrowband PL-L/PL-S, Philips) at 280-360 nm (peak 305-310 nm) with a power of 9 J/s was used for 10 minutes in the cell septum culture dish.Lysates were clarified from sonicated nuclei and HSF4-control-DNA, COIL-control-DNA, HSF4-UV-DNA or COIL-UV-DNA, complexes were isolated with antibody. In this experiment, cut run kit was used to extract (CST, USA). In short, prepare an appropriate number of living cells. First, combine concanavalin A beads with the target primary antibody HSF4 and coil respectively. After binding overnight, elute the beads with PAG mnase enzyme to digest and release the eluted DNA. Finally, DNA Purification Kit (CST, USA) was used to purify the obtained DNA fragment bound to the target protein. The obtained DNA was stored in DNA Evolution Buffer (cell signaling, USA).