Single cell RNA Seq to study the effects of Palbociclib in the mouse LSK cell compartment
Single cell RNA Seq to study the effects of Palbociclib in the mouse LSK cell compartment
Bone marrow cells of three wild type C57BL/6N mice were isolated. After lineage depletion, cells were pooled and distributed to either PBS or Palbociclib treatment. After 24h of cultivation, suspension cells were harvested and 67700 lineage-negative, Sca1-positive, cKit-positive (LSK) cells were sorted per sample by means of flow cytometry.  Cells were treated for 24h with 500nM Palbociclib or with PBS. Nucleic acid extraction was performed as part of the library construction. Libraries were generated using the Chromium NextGem Single Cell 5' v2 Kit solution (10X Genomics), according to the manufacturer's instructions