GSM6744377: anti-SIGLEC15 treated 1; Mus musculus; RNA-Seq
RNA was harvested using TRI regent (Sigma), and then fragment analyzer was used to examine the quality of extracted RNA. After quality check, samples were enriched with Oligo-coated beads,and then fragmentation buffer was added to generate transcripts.Then,complementary DNA strains were generated and collected for PCR replication.The samples of cNDA were checked according to protocols, and finally approved samples were loaded onto DNBSEQ-G50 platform for sequencing analysis.