GSM7062395: BaF3_gp130_IL6R_LIFR_LIF_b; Mus musculus; RNA-Seq
mRNA was isolated with NucleoSpin RNA (Macherey-Nagel, Düren, Germany; cat. no. 740955.250) according to vendor's manual. DNase digested total RNA samples used for 3´-RNA Seq analyses were quantified (Qubit RNA HS Assay, Thermo Fisher Scientific) and quality measured by capillary electrophoresis using the Fragment Analyzer and the 'Total RNA standard Sensitivity Assay' (Agilent Technologies, Inc. Santa Clara, USA). All samples in this study showed very high quality RNA Quality Numbers (RQN; mean = 10.0). The library preparation was performed according to the manufacturer's protocol using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD from Lexogen®. Input mount was 200 ng totalRNA. Bead purified libraries were normalized and finally sequenced on the NextSeq2000 system (Illumina Inc. San Diego, USA) with a read setup of SR 1x100 bp.