GSM6705272: TAPS Heart; Mus musculus; OTHER
Total DNA was extracted from cells or tissues using Phenol-chloroform DNA extraction protocol. Whole-genome TAPS libraries were constructed as previously described. In brief, 100 ng genomic DNA spiked with 1.0 % of fully CpG-methylated lambda DNA was sonicated in EB buffer and purified by DNA clean beads to select for the desired DNA fragment size. DNA fragments were oxidized by recombinant human TET2CD twice, and then reduced with pyridine borane. The library was amplified with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit.