GSM7324913: nHDF cells, non-targetting control, biol rep 2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini kit (Qiagen) according to manufacturer's protocol. 1 ug of total RNA was used for mRNA enrichement (Nex Poly(A) Beads 2.0) before the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using the Rapid Directional RNA-Seq Kit 2.0 (Perkin Elmer) following manufacturer's protocols.