GSM8062723: Guide 17 Induction 3, i3Neurons, DIV5 [i3_G17]; Homo sapiens; RNA-Seq
Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively. RNA library was prepared for sequencing with KAPA RNA HyperPrep with RiboErase. RNA-sequencing (NextSeq 2000) was carried out at a depth of 20 million reads per sample.