GSM7911965: Soft-Glass_3; Homo sapiens; RNA-Seq
Total RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer's instructions. RNA quantity and purity were verified using 2200 TapeStation system. Library preparation was performed using mRNA-Seq Library Prep Kit Lexogen following the manufacturer's instructions. Libraries were validated on TapeStation – HSD1000 ScreenTape®Dosage. WARNING: In contrast to most other library preparation protocols, SENSE libraries generate reads in a strand orientation opposite to the genomic reference.