Bulk RNA-seq of Drosophila melanogaster: Whole Head PTBI
mRNA was extracted from total RNA using polyA selection, followed by RNA fragmentation. Strand specific library was constructed by first-strand cDNA synthesis using random primers, sample cleanup and second-strand synthesis using DNA Polymerase I and RNase H. A single 'A' base was added to the cDNA fragments followed by ligation of the adapters. Final cDNA library was achieved by further purification and enrichment with PCR, quality checked using Bioanalyzer 2100. The libraries were sequenced (Single end 50bp reads) using the Illumina HiSeq2500, with 6 samples per lane, and final of over 20 million reads per sample