GSM8249244: CD8 T cells, scRNA_VDJ_lane4; Mus musculus; OTHER
To sort CD8+ T cells, single cell suspensions from ear pinnae skin of WT mice exposed to BHI-tween 14 days prior, or infected with HSV-1 7 days prior were stained with antibodies against CD45, lineage (CD11b, NK1.1, gdTCR), CD90.2, TCRb, CD4, CD8b, and DAPI for 30 minutes at 4°C. CD8+ T cells were sorted as DAPI- lineage- CD45+ CD90.2+ TCRb+ CD4- CD8b on a FACSAria. Each sample was additionally stained with an unique TotalSeq C hash tag antibdoy from Biolegend prior to sorting. Libraries were prepared using a Chromium Single Cell 5' Reagent kits following manufacturer's instructions including HTO and VDJ libraries. Libraries were then sequenced on an Illumina NextSeq 2000 (NextSeq 2000 P3 Reagents (200 Cycles), Illumina).