GSM7102841: BM-HL_3; Mus musculus; RNA-Seq
RNA was isolated from XY using the miRNeasy micro Kit (Qiagen) combined with on-column DNase digestion (DNase-Free DNase Set, Qiagen) to avoid contamination by genomic DNA. RNA and library preparation integrity were verified with LabChip Gx Touch 24 (Perkin Elmer). 3 µg of total RNA was used as input VAHTS Stranded mRNA-seq Library preparation following manufacture's protocol (Vazyme). Sequencing was performed on the NextSeq2000 instrument (Illumina) using P3 flowcell and 1x72bp single-read setup.