NextSeq 500 sequencing; Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach (ChIP-seq RNA-POLII)
Reconstructing the RUNX2 transcriptional hierarchy in thyroid cancer using a multi-layered network- based approach (ChIP-seq RNA-POLII)
40M cells were collected for Chromatin Immunoprecipitation. Cells were cultured in DMEM medium supplemented with 10% FBS and 1% P/S. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Libraries were obtained starting from 3ng of ChIP and Input samples following the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) protocol.