GSM8246979: Hypothalamus, PND14, rep1; Mus musculus; RNA-Seq
Total RNA containing microRNAs was isolated from hypothalamic tissues using the mirVana miRNA isolation Kit (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions. RNA concentration was evaluated by NanoDrop One spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA). Samples were proceeded using the QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) according to manufacturer's instructions. To evaluate library quality and size, each library was analyzed by High Sensitivity D1000 ScreenTape on an Agilent TapeStation 2200 (Agilent Technologies, Waldbronn, Germany). Libraries were quantify using the Qubit™ dsDNA HS Assay Kit on a Qubit 2.0 Fluorometer (Thermo Fisher Scientific, CA, USA).