GSM8421763: AtrxKO_rep1; Mus musculus; RNA-Seq
Snap-frozen tibias were pulverized using Tissuelyser II (Qiagen). Total RNA was extracted using cold chloroform and precipitated in cold ethanol. RNA samples were further purified with the Direct-zol RNA Kit (Zymo Research). RNA quality and quantity were measured by Bioanalyzer (Agilent Technologies). Strand specific cDNA libraries were generated with polyA selection using the Illumina TruSeq RNA Sample Prep Kit v2