GSM7052679: scRNAseq, cortical IN P15,  76; Mus musculus; RNA-Seq
Htr3a-GFP/Hmx3-Cre;R26tdTOM P15 brains were extracted in ice-cold HBSS. After sectioning with a Mcllwain tissue chopper, upper layers of the somatosensory cortex were micro-dissected under a stereomicroscope. For digestion, the Worthinton Papain Dissociation Kit and protocol were used with adaptations, see article methods for details. After digestion, cells were mechanically dissociated via pipetting in EBSS and centrifuged 5 minutes at 300g. Cell pellet was then suspended in a solution containing EBSS, AP5 Kynurenic acyd, ovomucoid, DNase and trehalose. Following a last centrifugation at 70g for 6 minutes, the pellet was resuspended in 1ml DMEM/F12 with FBS, HS, AP5, Kynurenic acid and trehalose and incubated with Hoechst 33342. Htr3a-GFP+/Hmx3-Cre;R26tdTOM+ and Htr3a-GFP+/Hmx3-Cre;R26tdTOM+ cells were FAC-sorted on a MoFloAstrios device gated for selecting independently GFP+/TOM+ or GFP+/TOM+ cells. Sorted cells were captured using integrated fluidic circuits and imaged prior to sequencing.