GSM6744341: TTTL_293T_2; Homo sapiens; RNA-Seq
RNA was harvested with Trizol reagent, followed by chloroform extraction and isopropanol precipitation. For TT-TL-seq genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. RNA for TT-TL-seq RNA was enriched with MTS-biotin and treated with oxidative-nucleophilic-aromatic-substitution chemistry as described in Schofield et al. Nature Methods 2018. For STL-seq, total RNA was treated with oxidative-nucleophilic-aromatic-substitution chemistryusing mCPBA as the oxidant and short capped RNA was enriched as described in Zimmer et al. Mol Cell 2021. TT-TL-seq libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413). STL-seq libraries were generated with custom oligos following methods described in Zimmer et al. Mol Cell 2021.