GSM7990419: primary tumor tissue 1; Homo sapiens; ncRNA-Seq
RNA was extracted using trizol.Agarose electrophoresis was used to check the integrality of total RNA samples, and then the samples were quantified on the NanoDrop ND-1000 instrument. Total RNA samples were first pretreated as following to remove some RNA modifications 1) 3'-adapter ligation; 2) 5'-adapter ligation; 3) cDNA synthesis; 4) PCR amplification; 5) size selection of  134-160bp PCR amplified fragments (corresponding to  14-40nt small RNAs). tRF & tiRNA Sequencing