title: 12-letter DNA (Val20) - BSn/BSc/PZ/XK/JV
design: Pooled sequencing run of the Val20 validation set for 12-letter DNA model (ATGCBSPZXKJV). XNA tailing and XNA ligation was used to build sequences with each of the XNA base pairs (B:Sn; B:Sc; P:Z, Xt:Kn, J:V). Ligation reactions for each base pair were carried out independently then pooled for sequencing reaction. Sequenced on a MinION r9.4.1 flowcell using SQK-LSK110. Data collected in this run forms was used for benchmarking recall of the 12-letter DNA model (ATGCBSPZXKJV) in a sequence context outside of what was used to build the model.