title: GSM7684103: M_UC-MSCs_P6; Homo sapiens; RNA-Seq
library_construction_protocol: The 6th passage UC-MSCs was collected and washed by 10 ml 1 x PBS+0.04 % BSA at 300 x g for 10 min. After cell counting, the cells were resuscitated to a concentration of 700-1200 cells/ul (viability was equal or greater than 85%) in a final solution of 1 x PBS + 0.04 % BSA prior to loading on the 10× Genomics Chromium platform. Chromium Single cell 3' Library and Gel Bead Kit V3.1 (10× Genomics, PN1000268) was used to generate single cell gel beads in emulsion (GEM). The captured cells were lysed, and the released RNA was reverse-transcribed with primers containing poly-T, barcode, unique molecular identifiers (UMIs) and read 1 primer sequence in GEMs. Barcoded cDNA was purified and amplified by PCR. The adapter ligation reaction was performed to add sample index and read 2 primer sequence. After quality control, the libraries were sequenced on Illumina Novaseq 6000 platform in 150 bp pair-ended manner.