title: RNA-seq of HY01: 30 degree celsius
design: Total RNA was extracted from Vibrio campbellii cells grown at 30 and 35 degree Celsius using TRIzolTM Reagent (Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA). The sequencing was conducted by Macrogen, Inc. (Seoul, Korea). The sequencing was conducted by Macrogen, Inc. (Seoul, Korea). Briefly, library preparation was performed using the NEBNext rRNA Depletion and TruSeq Stranded Total RNA Library Prep Gold Kit. Sequencing was then conducted using the NovaSeq 6000 system (Macrogen, Korea).