title: GSM6805334: Bone-marrow aspirates, ADT-derived/HTO-derived cDNA 8; Homo sapiens; OTHER
library_construction_protocol: Human bone marrow samples were thawed using prewarmed RPMI + 10% FBS and washed twice with cold PBS prior to labeling with unique TotalSeq C compatible HashTags (Biolegend). Samples were subsequently incubated with Human Fc receptor blocking agent (Biolegend) and labeled with DAPI for live cell FACS sorting using an Aria cytometer (BD Bioscience). Post sort, 150,000 cells were washed once with cold PBS prior to application of the TotalSeqC cocktail (Biolegend) and subsequently a custom ADT tagged U5 snRNP200 antibody (Biolegend).  Cells were washed three times with cold PBS and submitted to the Memorial Sloan Kettering Cancer Center Integrative Genomics Core for sequencing.