title: GSM7884285: 2_6_CD4_PanCK_WTA | 012 | CD4; Mus musculus; OTHER
library_construction_protocol: Murine lungs were harvested, formalin-fixed and paraffin-embedded. FFPE tissues were sectioned for GeoMx digital spatial profiling (DSP). For library preparation, Illumina i5 and i7 dual indexing primers were added to the oligonucleotide tags during PCR to uniquely index each AOI. Next generation sequencing (NGS) library preparation was performed according the NanoString Technologies published protocol (GeoMx DSP NGS Readout User Manual (nanostring.com)).