title: GSM6746079: Mini_screen_BV173_start_rep2; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed with Tris/SDS buffer supplemented with protease inhibitor, and DNA was precipitated with 40% isopropanol Region contatining sgRNA sequence was amplified with two sequenctial PCR and final product was gel purified--focused CRISPR mini-library was recovered