title: GSM7598121: HO, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: To isolate cells from ectopic bones, tissues were minced and digested with collagenase type I and DNase I at 37°C for 30 min. To isolate lung non-hematopoietic cells, the minced lung was placed in digestion solution containing collagenase Type I and DNase. The suspension was incubated at 37°C for 60 min under constant agitation. Cells were then incubated with red blood cell lysis buffer for 5 min. Preparations from all tissues were passed through a 100-μm cell strainer (Falcon) to remove debris. Libraries were constructed following the recommended protocol for the Chromium Next GEM Single Cell 3'Reagent Kits (v3)