title: Illumina HiSeq 2500 sequencing; Differential gene expression profiles associated with CAIX expression in patients with ER negative breast cancer
design: Differential gene expression profiles associated with CAIX expression in patients with ER negative breast cancer
library_construction_protocol: ER- BC patients with ductal carcinoma who underwent curative tumour resection surgery in Glasgow Royal Infirmary, Western Infirmary, or Stobhill Hospitals (Glasgow, UK) between 1995 and 1998 were included in the study. Resected tumour tissue was formalin-fixed and paraffin-embedded.  FFPE tissue was deparaffinised prior to tissue digestion. The tissue lysate was combined with detector oligos  which were annealed in immediate juxtaposition to each other on the targeted RNA template and ligated. Amplification of ligated oligos was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors.