title: GSM8196302: Compound PFECHS, Solvent Control Replicate 3; Danio rerio; RNA-Seq
library_construction_protocol: Embryos were exposed for 72 hours until 96 hpf. Exposure solutions followed semi-static renewal, where ~80% of the exposure solution was replaced per day. At the end of the exposure period, embryos were euthanized on ice before being pooled according to treatment group and replicate. 20 embyros were pooled per replicate resulting in three replicates of each treatment group for each compound (n = 15/PFAS) Total RNA was extracted using the Rneasy universal mini kit (Qiagen), with quality and quantity being assured using a tape station (Bioanalyzer). All sampels containing minimum 20ng/uL with a RIN > 8 were sent to GenomeQuebec for RNA-seq.