title: GSM7510067: WT, replicate 1, ATAC-seq; Mus musculus; ATAC-seq
library_construction_protocol: 50,000 LSK cells from WT or KO mouse models were used for each experiment. Cells were washed in PBS and then resuspended in lysis buffer containing 10 mM Tris-HCL (pH 7.4), 10 mM NaCl, 3 mM MgCl2 and 0.1% NP-40. Immediately after lysis, samples were treated with 2.5μl of Tn5 transposase (Illumina, #20034197) and tagmentation reactions were carried out at 37°C for 30 min. Tagmented DNA was purified using the MinElute PCR Purification Kit (Qiagen, #28004) and amplified using NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, #M0541) with the following PCR conditions: 72°C for 5 min, 98°C for 30 s, followed by 10 cycles of 98°C for 10 s, 63°C for 30 s and 72°C for 1 min. PCR-amplified ATAC-seq libraries were purified and size-selected using AMPure beads (Beckman Coulter, #A63881) with ratios of 0.55x-1.7x, enriching for DNA fragments of 170-800 bp.