title: GSM6883162: WT MI-3; Mus musculus; RNA-Seq
library_construction_protocol: Sex-balanced groups were collected at 7 days post-surgery (Sham or MI), hearts were harvested, full ventricles (above and below MI ligation) were isolated, and immediately homogenized in Trizol (Invitrogen) according to the manufacturer's protocol. Mouse tissue samples suspended in TRIzol were submitted to the University of Wisconsin Biotechnology Center (UWBC) Gene Expression Center (Research Resource Identifier - RRID:SCR_017757) for RNA extraction. Total RNA was purified following the recommendations of the Qiagen RNeasy Mini (Qiagen, Hilden, Germany) procedure, which included on-column DNase treatment. Total RNA was used as input material and libraries were prepared by following the SMARTer Stranded Total RNA Sample Prep Kit – HI Mammalian user manual (Takara Bio USA, Mountain View, CA, USA).