title: GSM7990857: V6.5 mTELSC, WGBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA and RNA were extracted from mESCs, mTBLCs, mTELCs, mTELSCs, mTSCs and TOs for highthrough sequencing, including ATAC-seq, CHIP-seq, WGBS and scRNA-seq. TRIzol reagent (Invitrogen) was used to isolate total RNA according to the manufacturer's instructions. 100 ng genomic DNA was used as input and was bisulfite-converted using the EpiArt DNA Methylation Bisulfite Kit (Vazyme). A total of 50, 000 viable cells were extracted using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme) for ATAC-seq.  A total of 50, 000 viable cells were extracted using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme). RNA-seq libraries were conducted using VAHTS Stranded mRNA-seq Library Prep Kits (Vazyme) .To assess chromatin accessibility of TELCs and TELSCs, ATAC-seq were conducted using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme) according to the manufacturer's instructions. Bisulfite-converted DNA was used to construct the library with the EpiArtTM DNA Methylation Library Kit V3 (Vazyme) according to the manufacturer's instructions. A total of 50, 000 viable cells were used for CUT&Tag experiments with the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) according to the manufacturer's instructions.