title: NextSeq 500 paired end sequencing; Six strains of Acinetobacter Baumannii: RNAseq data analysis
design: Six strains of Acinetobacter Baumannii: RNAseq data analysis
library_construction_protocol: Six strains of A. baumannii were studied. In detail, five field strains were isolated from blood samples (defined as strains 1, 2, 3, 4 and 6) of patients hospitalized at the University Hospital \"San Giovanni di Dio e Ruggi d'Aragona\" (Salerno, Italy). Total RNA was extracted from bacterial cultures using TRIzol, according to the manufacturer's instructions.  Quantification and quality control of the extracted RNAs were carried out using NanoDrop and Bioanalyzer. For RNA sequencing, libraries were prepared using 1 ?g of extracted RNA and the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit (Illumina, Inc., San Diego, CA, USA). The libraries were sequenced paired-end, 2x75 cycles on NextSeq500 platform at a concentration of 1.3 pM.