title: GSM7884095: RNA-seq_Bhlhe40_WT_Th2_rep1; Mus musculus; RNA-Seq
library_construction_protocol: All the cell subsets were harvested by cell sorting after staining. The total RNAs were harvested and purified by using Qiagen's miRNeasy micro kit (217084; Qiagen). Qiagen's DNase set (79254; Qiagen) was used for on-column DNase digestion. PolyA-tailed RNAs were purified from total RNA by using Dynabeads mRNA DIR ECT kit (61012; Ambion Life Technologies). The RNA-Seq libraries were sequenced with Illumina HiSeq system, and 50 bp reads were generated by the National Heart, Lung, and Blood Institute DNA Sequencing Core. ChIP-seq and RNA-seq