title: GSM7883438: slamseq_washout_dtag3; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted using Quick-RNA MiniPrep (Zymo Research) according to the manufacturer's instructions except including 0.1 mM DTT to all buffers. Thiol modification was performed by 10 mM iodoacetamide treatment followed by quenching with 20 mM DTT. RNA was purified by ethanol precipitation RNA-seq libraries were prepared using Roche Kapa mRNA HyperPrep sample preparation kits from 100ng of purified total RNA according to the manufacturer's protocol. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer's protocols. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on Illumina NovaSeq 6000 with paired-end 150bp reads