title: GSM8027889: Lacrimal Gland, Csk(CKO) and WT pooled. scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Lacrimal glands obtained from P1 pups underwent a PBS wash for 2-5 minutes and were digested with dispase (2000 units/ml) and papain (20 units/ml) for 30 minutes at room temperature. After gently removing the digestion enzyme mixture, the glands were washed with PBS and mechanically disrupted through vigorous pipetting until single-cell dispersion was observed under the microscope. They were pelleted with three washes in PBS and centrifugation at 3000 rpm at  4°C. Control and CskCKO mutant cells were treated with cell multiplexing oligos (CMO) in 24 accordance with the 10x Genomics 3' cellplex kit manual. The cells were then pooled together in a single collection tube for library preparation and subsequent sequencing at Columbia University's single-cell sequencing core.