title: EarlyBolting_leaf_mature_rep2
design: Total RNAs were extracted by cetyltrimethylammonium bromide and sodium dodecyl sulfate-lithium chloride methods (Vennapusa et al., 2020). Their quality and quantity were examined using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Two-microgram total RNAs were used to construct RNA-Seq libraries using an Illumina TruSeq RNA Sample Prep Kit according to the manufacturers instructions. Pooled libraries were sequenced using an Illumina HiSeq X platform with paired-end (PE) reads of 151 bp.