title: GSM7062731: Bulk LXR treatment, DMSO, Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Medium was removed and cells were lysed directly on plate in RNA lysis buffer and processed using the MagMAX mirVana Total RNA Isolation Kit (Applied Biosystems, cat#: A27828) according to the manufacturer's instructions. cDNA was generated from total RNA using SuperScript IV Reverse Transcriptase (Invitrogen, cat#:18090050). Bulk RNA-seq library preparation was performed with 500ng of total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, cat#:E7490) and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, cat#:E7420).