title: GSM7791001: YU-006 PDX, residual; Homo sapiens; RNA-Seq
library_construction_protocol: Subcutaneous PDX tumors were resected from mice and processed with tumor dissociation kit (Miltenyi Biotech, 130-095-929). The single-cell suspension was stained with human EpCAM antibody (BioLegend, 324209, 1:100) and LIVE/DEAD cell stain (invitrogen, L34971, 1:250), and subjected to FACS to isolate live tumor cells. The sorted cells were re-suspended in FACS buffer and loaded on the Single Cell A Chip together with RT Master Mix and barcoded gel beads to form nanoliter-scale Gel Beads in Emulsions (GEMs) through Chromium Controller. The single-cell cDNA libraries were constructed using 3' library prep kit v3 (10X genomics).