title: Shotgun metatranscriptomics of water: Produced water, absence of reagent, -78 degrees C, 24h, 1m high, second biological replica
design: rRNA were depleted with the QIAseq FastSelect 5S/16S/23S Kit, #335925 Qiagen. The libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, #E7760S NEB. Metatranscriptomic sequencing was performed using an Illumina HiSeq 2500