title: GSM7791546: Pa_47691_PeriInv_C; Homo sapiens; RNA-Seq
library_construction_protocol: FFPE sections (4 μm) were mounted on polyethylene naphthalate membrane (PEN) slides, were deparaffinized in xylene and then partially rehydrated in an ethanol gradient up to 75% EtOH. After that sections were counterstained with freshly prepared Cresyl Violet for 30 seconds, they were washed twice in 75% EtOH and air dried completely before proceeding to the microdissection using a UV-based LMD7 laser microdissection system (Leica Microsystems) at 20x magnification. Microdissected areas were collected by gravity into strip-tube caps. Microdissected areas were digested with 0.01 U of Proteinase K (ThermoFisher) for 1 hour at 60 C. 1 ul of 5 mM Proteinase K inhibitor (Calbiochem) was added to stop the reaction and samples were stored at -80 C until library preparation. They were then processed using the SMART-seq Stranded kit (Takara Bio USA, Inc.) according to the manufacturer's protocol removing the initial fragmentation step.