title: GSM7818928: Osmrfl/fl Mx1-Cre Veh 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using the RNeasy Micro kit (Qiagen), according to manufacturers' protocols, including the optional DNase digest step. RNA concentration and quality were assessed using the RNA 6000 Pico Assay (Agilent Technologies). Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v2-Pico (Takara), according to the manufacturer's protocol.  Briefly, the protocol entails RNA fragmentation and cDNA synthesis, addition of Illumina-specific adapters containing a unique barcode sequence for each library, cleavage of ribosomal cDNA with Takara's ZapR v2 and R-Probes v2, and PCR amplification of uncleaved fragments.  The quality and concentration of the libraries were assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively, according to the manufacturers' instructions. 2021:Libraries were sequenced 75 bp paired-end on an Illumina NextSeq 500 using the High Output Reagent Kit v2.5. 2022: Libraries were sequenced 150 bp paired-end on an Illumina NovaSeq 6000 using the S4 Reagent Kit v1.5.