title: GSM6918235: AFD, 25 degrees, rep2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: AFD-specific RNA was obtained using Translating Ribosome Affinity Purification. Briefly, frozen worms samples were lysed by grinding. The lysates were either used in an anti-EGFP ribosome immunoprecipitation followed by Trizol extraction (AFD samples) or RNA was extracted directly from lysates using Trizol (whole animal samples). 10 ng of RNA was used for library construction. Libraries were generated using the Smart-Seq v4 Ultra Low Input RNA kit followed by Nextera XT DNA Library Preparation Kit.