title: GSM6745639: PrimeEditing (Rep2); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified 24 hrs after editing using RNeasy Micro Kit (QIAGEN). DNase treatment was performed using RNase-free DNAse Set (QIAGEN), according to the manufacturer's instructions. RNA was quantified with Qubit 2.0 Fluorometer (Thermo Fisher) and its quality assessed by a 2100 Agilent Bioanalyser (Agilent Technologies). Minimum quality was defined as RNA integrity number (RIN) > 8. 300 ng of total RNA were used for library preparation with TruSeq Stranded mRNA (Illumina).