title: GSM8196740: JURKAT, control; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using TRIzol reagent. For the sodium oxamate inhibition experiment, after extracting total RNA from an equal number of cells, a diluted ERCC RNA spike-in mix (Invitrogen 4456740) was additionally incorporated as a control. For mRNA-seq libraries, poly-A mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries for sequencing were generated in accordance with the manufacturer's recommendations using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (NEB, #E7530).