title: GSM8027897: CHP-134, 6day 2uM ATRA, RNA-seq Replicate2 [CHP-134-6d-2]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol Reagent. For each sample, 150 ng of total RNA was used for library prep using Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus. RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR). For each sample, 200 ng of RNA were mixed with 4 µl of diluted ERCC RNA Spike-In Mix. All libraries were sequenced on the Illumina NovaSeq 6000 system.