title: GSM7328868: Rat CD4+ T-MBP cells, spleen NT control to Grk2 KO, rep R2; Rattus norvegicus; RNA-Seq
library_construction_protocol: The spleen was dissected and homogenized by passing through a metal strainer. Erythrocytes were removed by treating with ACK buffer for 3 min on ice and CD4+ T cells were enriched using the EasySep™ Rat CD4+ T Cell Isolation Kit (StemCell technologies), before purification by sorting based on their fluorescent markers, BFP for control and GFP for KO The library was prepared from total RNA using the Collibri 3' mRNA Library Prep Kits for Illumina Systems (Thermo Fisher). Amplification of transcripts was confirmed with Agilent Bioanalyzer on DNA 1000 Chips