title: GSM7064212: IPDC, biol rep 3; Serratia plymuthica; RNA-Seq
library_construction_protocol: RNA was extracted f by the hot phenol method using the TRI® Reagent protocol (Ambion) according to the manufacturer's instructions. RNA concentration was determined spectrophotometrically using a NanoDrop spectrophotometer (Thermo Scientific) and RNA integrity was assessed by agarose gel electrophoresis. Genomic DNA contamination was eliminated by treating total RNA with Turbo DNA‐free (Ambion), followed by a purification with RNeasy mini kit (Qiagen). RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit following manufacturer's protocols.