title: GSM7329719: RNASeq_dCas13_AUG_target_biol_rep_1; Homo sapiens; RNA-Seq
library_construction_protocol: Following FACS, in the cell harvest cycloheximide (100 μg/ml) was added to PBS, trypsin solution, and DMEM to stop ribosomal movement along mRNAs For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion, reverse transcription, cDNA circularization, and PCR amplification. RNA-Seq was performed by TruSeq Stranded Total RNA Library Prep Gold (Illumina).