title: Melipona RNA virome
design: Purified RNA were used for cDNA synthesis with the High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific, USA). The cDNA second strand was synthesized using Klenow fragment DNA polymerase (New England Biolabs, USA). Fragment libraries were further prepared with 1 ng of  double-stranded cDNA using the Nextera XT DNA Library Preparation kit (Illumina, USA) and paired-end sequenced (2150 nt) using the MiSeq Reagent kit v2 (Illumina, USA) on a MiSeq instrument (Illumina, USA).