title: GSM7791003: Adipose-Tregs_WT-KO-mix_CITE; Mus musculus; OTHER
library_construction_protocol: Labeled live CD3+ CD4+ Foxp3+ VAT Treg cells were FACS sorted and pooled; Srebf2WT/WT with Srebf2flox/flox (1:1) for one experiment and NCD with HFD (1:1) for a separate experiment. After pooling, single cell VAT Treg suspensions were loaded onto the 10X Genomics Chromium Controller using the Chromium NextGEM Single Cell 5' Library & Gel Bead Kit v2 to capture individual cells and barcoded gel beads within droplets. Following reverse transcription, the cDNA was prepared to create gene expression libraries and feature barcode libraries.