title: GSM7882925: Pancreatic Tumor Tissue, vehicle, 2 days, KA007; Mus musculus; RNA-Seq
library_construction_protocol: Tumor tissue was placed in a digestion buffer containing trypsin, DNase, and an enzymatic cocktail (Supplementary Table 7) and digested at 37°C for 42 minutes (37C_m_TDK_2 program) on a gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). Cell suspensions were then filtered through a 40 μm cell strainer (Corning, 431750) and red blood cells were removed by incubation with red cell lysis buffer (Millipore Sigma, 11814389001), before a final resuspension in 100 μL PBS + 0.01% BSA. Single Cell 3' v3, 10x Genomics