title: wild type 15 hour replicate 1  B
design: RNA was extracted from NYGCe001-A cells and iNs at multiple timepoints after differentiation using the Quick-RNA Mini kit (Zymo) and prepared using the TM3'seq method (Pallares et al., 2020). Sequencing was performed using 50 nt single-end reads on a NextSeq 550 (Illumina).