title: GSM7510179: B cells donor 3342 TP1, VDJ; Homo sapiens; OTHER
library_construction_protocol: Cryopreserved PBMCs were thawed, followed by isolation of B cells by negative magnetic selection. Naïve B cells (CD21+CD27-) and antigen-experienced B cells (all other B cells) were then isolated by fluorescence-activated cell sorting by first gating on live CD19+CD20+ cells, and subsequently on CD21 and CD27 expression. Antigen-experienced cells were mixed with a small proportion of naive B cells and then loaded onto the 10x Genomics controller. Standard 10x Genomics instructions were followed for the construction of 5' gene expression, antibody-derived tag (ADT), and V(D)J sequencing libraries.