title: RNA-seq of California Current System (Pacific Ocean) samples
design: Total RNA with was extracted from the filters using the NucleoMag RNA kit (Macherey-Nagel, Dren, Germany). Poly-A selected cDNA from total RNA was generated with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) which was then sheared with a Covaris ultrasonicator. The final sequencing library was then constructed with the NEBNext Ultra II DNA Library Kit (New England Biolabs). Libraries were subjected to paired-end 2150 bp sequencing on a NovaSeq 6000 instrument (Illumina).