title: GSM7029582: 283Scn (S79), L002; Homo sapiens; RNA-Seq
library_construction_protocol: The Sequencing and Genomic Technologies (SGT) Core Facility at Duke University performed RNA sequencing. The SGT Core Facility extracted total RNA from cell pellets using the RNeasy Plus Mini Kit from Qiagen (catalog #: 74134). mRNA was enriched from total RNA and reverse-transcribed into cDNA to build sequencing libraries using the KAPA mRNA HyperPrep Kit from Roche (KK8581). Libraries were pooled to equimolar concentrations and sequenced on the NovaSeq 6000 S2 flow cell to produce 50 bp paired-end reads.