title: GSM7029823: L_mouse3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using RNeasy Plus Micro Kit (Cat#74034). Libraries were constructed following the Smart-seq2 protocol (Picelli S, et al. Nature. 2014) with minor modifications. First-strand cDNA were synthesized adding oligo dT primer and template switching oligo. Then, full-length ds cDNA were amplified by IS-PCR. Amplified cDNA were purified using magnetic beads. cDNA libraries were constructed using Nextera XT DNA Library Prep Kit (illumina, Cat#.FC-131-1096), and finally purified and size selected using magnetic beads. Libraries were sequenced on the Illumina NextSeq2000 following the manufacturer's protocols.