title: GSM6918299: Post_Vaccination Stamaril D3 CO369_D3; Macaca fascicularis; RNA-Seq
library_construction_protocol: Whole blood from NHPs was collected in PAXgene tubes (BD762165 –Ozyme) and stored according to the manufacturer's protocol. Total RNA was extracted with the PAXgene 96 Blood RNA kit (Qiagen-762331) according to manufacturer's instructions on Tecan Evo150 workstation Extracted RNA samples were processed using the GLOBINclear™-Human kit (ThermoFisher - AM1980) for the depletion of the alpha and beta globin mRNA according to manufacturer's instructions. Sequencing libraries were prepared with the TruSeq mRNA stranded kit (Illumina - 20162122/20146602) using 300 ng of total globin-depleted RNA according to manufacturer's instructions.