title: GSM7883340: atac_dmso_41; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq was performed as described (Buenrostro et al., 2015). Total 50,000 cells were washed with cold PBS and lysed using cold lysis buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3mM MgCl2 and 0.1% IGEPAL CA-630). The pellet was then resuspended in the transposition reaction mix (25 μL 2× TD buffer, 2.5μLTn5 Transposes (Illumina) and 22.5μL nuclease-free water) and incubated at 37°C for 30min. Immediately following transposition incubation, DNA was purified using a Qiagen MinElute Kit. ATAC-seq libraries were generated using Ad1_noMX and Ad2.1-2.4 barcoded primers (Buenrostro et al., 2013) and were amplified for 6-9 total cycles. All libraries were sequenced on Illumina NextSeq500 with pair-end reads (2X 42bp, 8bp index).