title: GSM7818971: XRN2 DEGRON, NO RESCUE, AUXIN, REP1; Schizosaccharomyces pombe; OTHER
library_construction_protocol: Total RNAs were extracted by a standard hot phenol method from 4-thiouracil labelled cells (S.pombe mixed with S.cerevisiae as a spike-in). Library was prepared following TT-seq (chem) protocol. Briefly, RNA was treated with DNAse, subjected to NaOH fragmentation, followed by RNA biotinylation and purification on streptavidin beads. Purified RNA (50ng) was used in library preparation following manufacture recommendations for NEBNext ultra II directional RNA library prep kit for Illumina.