title: GSM7151664: R35A3_PU.1_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: To obtain samples for the ChiP-seq experiments biological duplicates of primary B cells expressing either C/EBPαWT ER or C/EBPαR35A ER were induced for 3h with E2 and 100,000 cells per sample processed by ChIPmentation, as described (Schmidl et al., 2015) (https://www.nature.com/articles/nmeth.3542). Three µl of anti-C/EBPa antibody (Santa Cruz sc-61 14AA) or anti-PU1 (Santa Cruz sc-352 T21) were used per immunoprecipitation. Tagmentation of immobilized C/EBPa or PU1 enriched chromatin was performed for 5 min at 37 degrees in a 25µL transposition reaction mix Libraries were prepared with an Illumina TruSeq ChIP Library Preparation Kit and after quality control on Bioanalyzer sequenced (1 × 50 bp) on an Illumina HiSeq2500 instrument.