title: GSM8027781: U-2 OS cells, Vpr_Wt_36_13_UDI59_S13_R1_001; Homo sapiens; RNA-Seq
library_construction_protocol: At 36 hours post treatment, total RNA from U2OS cells was isolated using TRIzol® reagent (Invitrogen). RNA integrity was assessed using the Bioanalyzer TapeStation 4200 RNA High Sensitivity (Agilent). Library Preparation was completed using the KAPA mRNA HyperPrep Kit (Illumina) enriching for Poly(A) RNA with magnetic oligo-dT beads and attaching unique dual indexed adapters. Quality control of the library was done with the Bioanalyzer TapeStation 4200 D1000 High Sensitivity (Agilent). RNA was sequenced using the Hiseq3000 1x50 at the UCLA Technology Center for Genomics & Bioinformatics core.