title: GSM7791187: HCC827EV, vehicle, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Cell nuclei were extracted with resuspension buffer containing 0.1% NP-40, 0.1% Tween-20 and 0.01% Digitonin, followed by tagmentation with transposase (illumina, 20034197). The tagmented DNA was purified with DNA cleanup kit (Zymo, D4014). The tagmented DNA was amplified using NEBNext Master Mix (NEB, M0541S) to construct illumina sequencer-compatible libraries. ATAC-seq DNA libraries was purified with AMPure XP magnetic beads (Beckman Coulter, A63880) and quantified with KAPA Library Quantification Kit (KAPA, KK4854).