title: GSM7601075: Human in vitro-derived MPC (Day 13 Dex cells); Homo sapiens; RNA-Seq
library_construction_protocol: For the preparation of cells for single-cell sequencing we used the Chromium Single Cell 3′ library and gel bead kit v2 protocol. Single cell libraries from Day 13 and Day 16 treated and untreated samples were generated using the Chromium Single Cell 3′ library and gel bead kit v2 (PN #120237) from 10x Genomics. Briefly, to reach a target cell number of 3,000 cells per sample, 5,000 cells per sample were loaded onto a channel of the 10x chip to produce Gel Bead-in-Emulsions (GEMs). This underwent reverse transcription to barcode RNA before cleanup and cDNA amplification followed by enzymatic fragmentation and 5′adaptor and sample index attachment. Libraries were sequenced on the HiSeq4000 (Illumina).