title: GSM7224022: iPSC neurons, FTDscr-2; Homo sapiens; RNA-Seq
library_construction_protocol: For neuronal differentiation of NPCs, expansion media was changed 2 days after splitting to neuronal induction media consisting of N2B27 medium supplemented with 1 μM PMA, 200 μM AA and 3 μM CHIR 99021. After 6 days of differentiation the neuronal induction media was replaced by neuronal maturation media consisting of N2B27 with 20 ng/ml BDNF, 10 ng/ml GDNF, 1 ng/ml TGF-β3 (Peprotech), 200 μM AA, and 500 μM dbcAMP (Sigma Aldrich). Media was changed every other day and cultures were analyzed after three weeks in maturation conditions. Poly-A pull-down was applied to enrich for mRNAs from total RNA samples followed by library construction using Illumina TruSeq chemistry. Libraries were sequenced at the Columbia Genome Center using Illumina NovaSeq 6000. Samples were multiplexed in each lane yielding targeted number of paired-end 100bp reads for each sample. bulk-RNA sequencing