title: GSM7510893: Brain, Control, Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation was performed using the Qiagen Rneasy Mini Kit and protocol per manufacturer recommendations Library preparation was completed using theNEBNext Ultra II Directional RNA Library Prep Kitand protocol (P/N: E7760L) per manufacturer recommendations. RNA samples underwent mRNA enrichment using oligo(dT) beads, followed by fragmentation using divalent cations under elevated temperature. First-strand cDNA synthesis was performed using random hexamer primers, while second-strand cDNA synthesis was carried out using DNA Polymerase I and RNase H. End repair, A-tailing, and adapter ligation were performed to generate the final cDNA library.