title: GSM8027059: EedKO_rep2; Mus musculus; ATAC-seq
library_construction_protocol: 50,000  cells were lysed in 50 µl cold ATAC lysis buffer (10 mM Tris·Cl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630) followed by centrifugation at 500 g at 4°C to isolate nuclei. Nuclei were resuspended in 50 µl transposition reaction mix with Nextera Tn5 Transposase (Illumina, FC-121-1030) and incubated for 30 min at 37°C. Transposed DNA was purified using columns (Qiagen, 28004) and amplified using high-fidelity 2X PCR Master Mix Illumina sequencing libraries were generated by end repair, 3' A-addition, and Illumina sequencing adaptor ligation.