title: GSM7790927: NES, PRDM6, Replicate 3; Homo sapiens; ATAC-seq
library_construction_protocol: Preparation of ATAC-seq libraries and sequencing was performed as described [PMCID: PMC7580199 DOI: 10.1016/j.xpro.2020.100079], with slight modifications. In brief, 100,000 cells per sample were harvested and lysed in ATAC-seq lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1 % (w/v) IGEPAL CA-630). The transposition reaction was performed using Tagment DNA TDE1 Enzyme and Buffer Kit (Illumina) and 4.7 µL TDE1 Tagment DNA enzyme per sample in a 50-µL total reaction volume. Zymo DNA Clean and Concentrator Kit was used for cleanup of the transposition reaction. Quantitive PCR and limited-cycle PCR was performed using NEBNext Ultra II master mix (New England Biolabs), SYBR green and 0.5 µM of sample-specific P5/P7 barcoded primer mix (New England Biolabs). Bead clean-up and purification was performed using SPRI beads (Beckman Coulter). Agilent TapeStation 4200 was used to check for quality of sequencing libraries.