title: WGS of aTC supplemented pKD_MORC P. falciparum NF54::pCRISPRint
design: gDNA was extracted and purified using the DNeasy Blood & Tissue kit (Qiagen). PCR analysis was used to genotype the transfected lines then PCR amplification was conducted using KAPA HiFi HotStart ReadyMix (Roche). gDNA was fragmented using a Covaris S220 ultrasonicator and libraries were generated using KAPA LTP Library Preparation Kit (Roche).