title: GSM6805015: L3_ClampNull_F06; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Total RNA extracted using Trizol (Invitrogen) from 3 third instar larvae each. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.