title: GSM8252004: Ctnnb1_CtrlwithIFNr; Mus musculus; RNA-Seq
library_construction_protocol: Organoids were digested with TrypLE for 10-30min to generate single cell suspension. Single cells run through flow cytometery to move dying cells and ensure viability above 90%. Libraries were prepared following the manufacturer's protocol for the 10x Genomics Chromium Single Cell RNA Expression Kit v3.1. Briefly, a single-cell suspension was obtained from the sample tissue and quantified to ensure a target concentration of 1000 cells per μl with over 90% viability. Cells were loaded onto a Chromium Chip along with reagents from the kit, and barcoding was performed using the Chromium Controller. Following barcoding, cDNA was synthesized, amplified, and then purified using magnetic separation. The cDNA underwent library construction which included enzymatic fragmentation, size selection, and PCR amplification. The final library quality was assessed using a bioanalyzer before sequencing.