title: GSM7149889: Pancreatic differentiation 2D; Homo sapiens; RNA-Seq
library_construction_protocol: For 2D samples, differentiated cells were dissociated using TrypLE Select (Gibco) for 10 minutes. For 3D samples, cell laden hydrogels were treated with 1 hour Type 1 collagenase treatment (50 U/mL), and cells were dissociated with TrypLE Select for 15 minutes. Following, the cells were washed to remove debris and filtered with 40 µm strainer. Cells were washed three additional times with DPBS with 2%BSA (1000 rpm x 3 minutes). Approximately, 10,000 single cells were recovered, and cDNA libraries were generated utilizing the v3.1 Next GEM Single Cell 3' reagent kit and a Chromium single cell system (10x Genomics) and sequenced on a NovaSeq 6000 sequencer (Illumina) at the Center for Medical Genomics at Indiana University School of Medicine.