title: GSM6805281: HEK293T_ABE7.10.rep2; Homo sapiens; RNA-Seq
library_construction_protocol: HEK293T cell were seeded into 24-welll plate (Corning) one day before transfection. After confluency of cell reach 80%, transfection of base editor and gRNA as plasmid DNA or RNA were done with Lipofectamine 2000 (Thermo Fisher Scientific), as described previously. After incubating 72h at 37 °C, cell were washed with 1x PBS and then lysed with TRIzol (THermo Fisher Scientific). Total RNA extraction, mRNA purification and RNA library preparation were done using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs), following the manufacturer's instruction. Final library were quantified using Library Quantification Kit (KAPA Biosystems) and sequenced on a NextSeq 500/550 High Output Kit v2.5 (75 Cyclies) as 85nt single-end reads.