title: GSM7508902: Mouse_Face_E12_5_rep1; Mus musculus; ATAC-seq
library_construction_protocol: Face tissue was dissected from mouse embryos for each of the developmental stages e10.5-15.5, and flash frozen. Nulcei were isolated, permeabilized, suspended in tragmentation buffer, and distributed in a 96-well plate. TnT transposomes were added and upon termination of the TnT reaction, the nuclei were re-sorted in the 96-well plate. A Biomek i7 Automated Workstation (Beckman Coulter) was used for performing downstream steps. Libraries were sequenced on a NextSeq500 or HiSeq4000 (Illumina) using custom sequencing primers.