title: The mRNA sequence of quinoa leaves
design: Enriching mRNA with polyA structure in total RNA using Oligo (dT) magnetic beads and using ion disruption to break the RNA into fragments of approximately 300bp in length. Select a fragment with a length of 300bp because the length of the splice is fixed. If the length of the broken fragment is shorter, it will lead to a higher proportion of splice sequences, thereby reducing the proportion of valid data; If the length of the interrupted fragment is long, it is not conducive to cluster generation during the online sequencing process. Using RNA as a template, the first strand of cDNA was synthesized using 14-base random primers and reverse transcriptase, and the second strand of cDNA was synthesized using the first strand of cDNA as a template.