title: GSM8289638: SINRV-TGM1-ΔTGM1_K81p7_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: Fully confluent keratinocytes were detached, and cells were accurately resuspended to obtain a single-cell suspension in PBS containing 0.04% BSA. About 8 x 104 cells of SINγ-RV-TGM1-transduced keratinocytes sample were subjected to nuclei isolation and DNA fragmentation according to manufacturer's instructions (#CG000338 Rev F, 10X Genomics). About 15,000 nuclei were loaded into the Chromium Next GEM Chip J using the Single Cell Multiome ATAC + Gene Expression kit (10X Genomics). The sample was pre-amplified and half-divided to generate the Illumina sequencing ATAC and GEX libraries. Sequencing was performed on the NovaSeq6000 SP platform following the 10X Genomics instruction for read generation (150PE), reaching on average 67,000 reads per cell for the GEX library and 213,000 reads per cell for the ATAC library. Single Cell Multiome ATAC + Gene Expression (10X Genomics)