title: GSM7734751: Y3.3_NTT/108155; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were isolated, cut into pieces, and digested for approximately 1.5 h at 37°C with collagenase A (1 mg/ml, Roche) and DNAse (20mg/ml, Worthington) in RPMI-1640 media. Digested tumors were strained through a 70um filter and resuspended in FACS buffer (0.5% BSA, 2mM EDTA). FC-block with anti-CD16/32 (clone 93, BioLegend) was performed for 10 min at 4°C to avoid unspecific antibody binding and staining was performed for 30 min at 4°C degrees. Samples were stained for CD45+ cells and live dead exclusion was performed by staining with the fixable viability dye eF780 (1:1000, eBioscience). Dissociated cell concentration after FACS was measured using NucleoCounter NC250 (Chemometec) following the manufacturer's instructions. Library was constructed according to manufacturer's protocol using the Chromium Next GEM Single cell 3' kit, 10x Genomics