title: Single-cell RNA sequencing in HBV-associated cirrhosis
design: Single cells were loaded onto the Chromium platform (10X Genomics, USA) to construct single-cell gel beads in emulsions. The libraries were prepared using Chromium Single Cell 3 v3 reagent kit (10X Genomics) and were sequenced using an Illumina NovaSeq PE150 platform following the user guide provided.