title: GSM7031292: HCT116_WT-CSTF2+Serum; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq,cells were crosslinked with 1% formaldehyde for 10 min and quenched with glycine for 5 min at the room temperature. Fixed cells were sonicated and immunoprecipitated with the mixture of beads and antibody overnight at 4 ℃. ChIP-DNA was purified using PCR purification kit (Qiagen) and performed for qPCR using iTaq™ Universal SYBR® Green Supermix (Bio-Rad) on CFX96 (Bio-Rad). The ChIP-seq libraries were prepared with VAHTS Universal DNA Library Prep Kit for Illumina V3