title: GSM6652286: ATCC cell line CRL-3240 UPCI:SCC152; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed 3 times in 1x PBS, and trypsinized off their plates and resuspended in cold complete Emedia. Cells were pelleted by centrifugation, washed once with cold PBS and repelleted. RNA was harvested using Rneasy mini kit (Qiagen). Approximately 500 ng of total RNA was used for the construction of sequencing libraries. Illumina - TruSeq Stranded mRNA Library Prep kit