title: GSM8196738: MDA-MB-231GlnIND cells, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA isolation was carried out using the RNeasy Mini Kit (QIAGEN) and QIAshredder (QIAGEN) from cells that were snap-frozen on plates. Libraries were constructed using the Kapa mRNA HyperPrep kit (Roche Molecular Systems). The total RNA fraction was enriched for mRNA via oligo-dT beads, and the first cDNA strand was synthesized using random priming, following by the combined second strand synthesis and A-tailing. Illumina adaptor ligation was then performed, followed by library amplification utilizing high-fidelity, low bias PCR.