title: GSM8027610: MI-2; Rattus norvegicus; RNA-Seq
library_construction_protocol: total RNA was purified from the infarct zone of Sham, MI and MI/R using RNeasy RNA isolation Kit (Qiagen) coupled with an on-column DNase I digestion to eliminate residual DNA. The mRNA was purified from 2 μg of total RNA. libraries for massive parallel sequencing were constructed with NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB). Library quality was assessed with Agilent Bioanalyzer 2100 system. Next-generation sequencing was performed on an Illumina HiSeq 4000 platform.