title: GSM7437674: E11_5_CVG_POU4F1_Rep1; Mus musculus; OTHER
library_construction_protocol: E11.5 CVGs were dissected and extracted nuclei immediately by incubating in the NE Buffer for 10 min on ice. To prepare the library, 21ul DNA fragments was removed to PCR tube, to which 2 ul of barcoded i5 and i7 primers and 25 ul CUTANA High Fidelity 2x PCR Master Mix (EpiCypher, #15-1018) were added. PCR was performed using the following conditions: 72 °C for 5 min, 98 °C for 30 sec, and 20 (can be changed depending on experience with the sample) cycles of 98 °C for 10 sec, 63 °C for 10 sec, followed by an extra 1 min extension at 72 °C. The PCR product was analyzed on an Agilent Fragment Analyzer. Combined DNA libraries were cleaned up using AMPure XP beads (Beckman Coulter, #A63880) following the manufacturer's instructions.