title: GSM7147282: CN+ONX0914 rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Extract RNA reagent (Evrogen). The concentration of RNA was measured with a Qubit Fluorometer (Invitrogen). The quality of RNA was determined with an Agilent BioAnalyzer 2100 using an RNA 600 nano kit. The RNA Integrity Number (RIN) of all RNA samples was not less than 8. Libraries for RNA-seq were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's guidelines. One hundred bp single-end sequencing was conducted on an Illumina NextSeq 2000 platform.