title: GSM8326761: 3733A, MP+BP; Mus musculus; RNA-Seq
library_construction_protocol: different state of SS tumor of mice were harvested  at the point of morbidity. Half of the tissue was digested as described before using mouse tumor dissociation kit. (Miltenyi Biotec). The corresponding nearby tissue were embedded in OCT for H&E staining. After dissociation the cell suspension was filtered through 40 um strainer and then treat with red blood cell lysis solution (Miltenyi Biotec). At last, cells were resuspended in PBS+0.04%BSA at a concentration of ∼1,000 cells/μL ready for single-cell sequencing. 10,000 cells per samples were targeted. scRNA-seq library preparation processes were guided by Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide scRNA-seq library preparation processes were guided by Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204) followed by Illumina NovaSeq with paired-end dual-indexing (28 cycles Read 1, 10 cycles i7, 10 cycles i5, 90 cycles Read 2).