title: GSM8251942: 2-RHm17 CD8; Macaca mulatta; RNA-Seq
library_construction_protocol: Cryopreserved LN samples from 5 A*01+ animals at D42 p.i. were thawed and stained with the following antibodies: anti-CD8-FITC (clone RPA-T8, BD Biosciences), anti-CD4-BV650 (clone OKT4, Biolegend), anti-Gag-PE (CM9 tetramer, NIH Tetramer Facility at Emory University, anti-CD95-PE/Cy7 (clone DX2, BD Biosciences), anti-CD28-APC (clone CD28.2, BD Biosciences), anti-CD3-APC/Cy7 (clone SP34-2, BD Biosciences). A minimum of 10,000 SIV-specific CD8 from each animal was sorted using a FACS Aria II system and loaded onto 10X Chromium Controller. Single cells were partitioned into droplets (Gel Beads in Emulsion: GEMs) using Chromium NextGEM Single Cell 5' Library & Gel Bead kits on the 10X Chromium Controller. The resulting cDNA was amplified and libraries were prepared for transcriptomic analysis according to manufacturer instructions. Gene expression libraries were sequenced as paired-end 26x91 reads on an Illumina NovaSeq6000 targeting a depth of 50,000 reads per cell.