title: GSM8248704: IPSCs, control, CUT&Tag, rep01; Homo sapiens; OTHER
library_construction_protocol: Cells was rinsed with PBS and dissociated with 0.5mM EDTA. Resuspended cells were attached to Concanavalin A beads (Bangs Laboratories, IN, USA) and then were incubated with primary antibody against H3K27ac (Active motif, CA, USA). After washed, cells were incubated with pA-Tn5 (Vazyme, China). After quenching the fragmentation, the cells were added with proteinase K. Post-incubation, a phenol–chloroform–isoamyl alcohol mix wasused to extract DNA. The samples were incubated at -80°C freezer and subsequently centrifuged. The pellet was air dried and resuspended in a desired amount of EB buffer. DNA concentration was estimated using the Qubit. PCR amplification was performed using universal N5 primer and indexed N7 primer and PCR cycle number was optimized. Amplified products were purified using 1: 1.3X Ampure bead.