title: GSM7328409: HAEC 0Gy rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the TRIsure solution (Bioline, London, UK) according to the manufacturer's instructions. RNA quality was assessed with an Agilent 2100 bioanalyzer and an RNA 6000 Nano Chip (Agilent Technologies, Amstelveen, Netherlands), obtaining the RNA integrity number. Total RNA quantification was performed using a NanoDrop 2000 spectrophotometer (ND-2000; Thermo Fisher Scientific, Inc.). RNA-seq was conducted on high-quality RNA samples (RNA integrity number > 7) from HAEC cells exposed to 0Gy (no radiation), 0.1Gy, 0.5Gy, or 2.0Gy. Samples were multiplexed into lanes and sequenced using a HiSeq 4000 system (Illumina, San Diego, CA, USA).