title: GSM7225037: Lesioned WM_Old_rep1; Mus musculus; RNA-Seq
library_construction_protocol: The frozen white matter tissues were homogenized in the cold Nuclei EZ Prep lysis buffer (Millipore Sigma) in a 2ml Dounce grinder, followed by clean-up using OptiPrep Density Gradient Medium (Millipore Sigma) (Corces et al., 2017). The nuclei recovered from the interface of OptiPrep medium were used for snRNA-seq preparations. Single-nucleus RNA-seq libraries were prepared using Chromium Next Gem Single Cell 3' v3.1 Reagents following the manufacturer's instruction (10X Genomics).