title: GSM8152758: zebrafish sperm, biological repeat  2; Danio rerio; RNA-Seq
library_construction_protocol: Sperm samples were collected from five zebrafish, three GSCT zebrafish and five wild-type grass carps. 0.5 μL sperm from each fish was diluted into 20 μL Hank's buffer. For one sample, 1μL of diluted sperm was used to extract RNA and synthetize cDNA in one reaction using the single cell full length mRNA-amplification kit (N712, Vazyme) following manufacturer's protocols. cDNA was used to prepare DNA library for sequencing using the DNA Library Prep Kit for Illumina (TD504, Vazyme) following manufacturer's protocols.