title: GSM8196124: IP NC_1; Homo sapiens; RNA-Seq
library_construction_protocol: Vitreous body samples were obtained during pars plana vitrectomy of 90 diabetic retinopathy patients (DR group) and  90 non-diabetic retinopathy patients (NC group, including rhegmatogenous retinal detachment, macular hole, epimacular membrane). 30 samples of each group were combined and entitled as DR_1-3 and NC_1-3, respectively.  These samples were flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. The m6A RNA was concentrated via immunoprecipitation with anti-m6A antibody before sequencing. RNA libraries were prepared for sequencing using standard Illumina protocols