title: GSM7062913: MCF-7_DMSO; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the innuPREP RNA Mini Kit (Analytik Jena, Jena, Germany) following the manufacturer's protocol. Oxford Nanopore genomic library preparation protocol SQK-PCB109 was followed using 50 ng of total RNA as input. The samples were barcoded according to protocol with 16 PCR cycles. Libraries were run for 72h according to the guidelines of the manufacture using Mk1B-MinION sequencer and R9.4.1 flow cells (FLO-MIN106D). Sequencing run monitoring and real-time data acquisition were performed using the MinKNOW software suite v22.03.6.