title: Illumina HiSeq 4000 paired end sequencing; KAS-seq of OE19 cells
design: KAS-seq of OE19 cells
library_construction_protocol: OE19 cells were trypsinised and collected OE19 cells nucleic acid extraction for KAS-seq was performed as described in Wu T, Lyu R, You Q, He C. Kethoxal-assisted single-stranded DNA sequencing captures global transcription dynamics and enhancer activity in situ. Nat Methods. 2020 17:515â€“523  Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters