title: The genome sequence of E. coli JM101
design: The high molecular weight DNA sample was optionally fragmented or size-selected to obtain double-stranded DNA fragments with appropriate size. Then DNA fragments were end-polished, nick repaired and A-tailed. The 1D library itself was produced by ligating sequencing adapters onto double-stranded DNA fragments. After purification with AMPure XP beads, the prepared library was ready for library quality control.