title: GSM7224091: Female_SW_Pax_rfp_rep1; Mus musculus; OTHER
library_construction_protocol: MicroSpin S-400 columns (GE Healthcare) were used to isolate ribosome bound RNAs. After rRNA was removed using RiboCop rRNA depletion kit (Lexogen), footprints were dephosphorylated then size selected (28-34nt) by PAGE on 15% TBE-Urea gels (Bio-Rad). Footprints were generated by SMARTer smRNA-Seq kit for Illumina (TaKaRa). RNA abundance was quantified using the Quantseq 3ʹ mRNA-Seq library kit (Lexogen). The concentrations of purified libraries were quantified using Qubit (Invitrogen)