title: GSM7777578: Prmt1KO-IFNy-Rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 1x10^6 cells using the RNeasy Mini Kit (Qiagen, Cat#74106) in accordance with the manufacturer's instructions and quality checked using a 4200 TapeStation machine. One microgram of total RNA per sample was used for NGS library preparation (Illumina TruSeq RNA, Cat#FC-122-2002) as per the manufacturer's instructions and quality checked using a 4200 TapeStation. NGS libraries were pooled and sequenced using a P2 100-cycle Illumina sequencing kit (Illumina, Cat#20046811) on the NextSeq 2000 sequencing system with paired-end 66bp reads.