title: GSM7031727: hs_Sub6_pear_glut_CTCF; Homo sapiens; ChIP-Seq
library_construction_protocol: Fat biospsies were collected and SVF were isolated by centrifugation. For ATAC-seq, nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina).DNA was purified with MinElute PCR Purification Kit (Qiagen). For ChIP-seq, lysates were clarified from sonicated nuclei and histone/CTCF or RNAPII-DNA complexes were isolated with antibody. ATAC-seq and ChIP-seq libraries were prepared and sequenced using standard Illumina protocols for HiSeq 2500 instrument.