title: GSM6805277: CBMNC-ADT; Homo sapiens; RNA-Seq
library_construction_protocol: To collect HUVECs from human umbilical cord, Liberase solution was infused into the vessel to release ECs from the basement membrane. The solution containing digested ECs was flushed into tubes for EC recovery. To collect CBMNCs from human umbilical cord blood samples, Ficoll-Paque was added to the anti-coagulated blood (diluted in PBS) and cell separation was performed according to the manufacturer's protocol. Both HUVECs and CBMNCs were magnetically sorted with CD34 beads and resuspended with DMEM/10%FBS. Library was performed according to the manufacter's instructions (single cell 3' v2 and v3 protocol, 10x Genomics).