title: GSM6917520: CUT&RUN IgG, Transient ph-KD D11 AEL, rep1; Drosophila melanogaster; OTHER
library_construction_protocol: Cut&Run experiments were performed as described by Kami Ahmad in protocols.io (https://dx.doi.org/10.17504/protocols.io.umfeu3n) with minor modifications. 50 eye discs were dissected in Schneider medium, centrifuged for 3 min at 700g and washed twice with wash+ buffer before addition of Concanavalin A-coated beads. MNase digestion (pAG-MNase Enzyme from Cell Signaling) was performed for 30 min on ice. After ProteinaseK digestion, DNA was recovered using SPRIselect beads and eluted in 50ul TE. DNA libraries for sequencing were prepared using the NEBNextÂ® Ultraâ„¢ II DNA Library Prep Kit for Illumina. Sequencing (paired-end sequencing 150bp, approx. 2Gb/sample) was performed by Novogene (https://en.novogene.com/).