title: GSM8028010: HC11_undifferentiated_antiVangl2_rep03; Mus musculus; OTHER
library_construction_protocol: We collected 100,000 cells from three independent dishes of HC11 cells at undifferentiated, confluent, and differentiated states. CUT&RUN was performed on HC11 cells using a rabbit anti-VANGL2 antibody (1:50, Millipore-Sigma, Burlington VT, USA; ABN2242) and isotype control IgG (Cell Signaling Technologies, Danvers, MA, USA;  2729) and the CUT&RUN Assay Kit (Cell Signaling Technologies, Danvers, MA, USA;  86652). The resulting DNA fragment samples were generated into libraries using the SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® (Cell Signaling Technologies, Danvers, MA, USA;  56795) and SimpleChIP® ChIP-seq Multiplex Oligos for Illumina® (Dual Index Primers) (Cell Signaling Technologies, Danvers, MA, USA;  47538). Samples were sequenced using the NovaSeq 6000, 50PE (UC Berkeley, Berkeley, CA, USA).