title: Pt17-NS-iTx-RECIPIENT-MLN-Pre-tx10-05-2015-10M-VIAL-rep4
design: Genomic DNA was extracted from FACS-sorted recipient HLA+ CD19+ and/or CD138+ cells from post-Tx biopsies and blood samples using the Qiagen Gentra Puregene blood kit (Qiagen, Valencia, CA, Cat. No. 158389) as previously described11. DNA quality and yield were evaluated by spectroscopy (Nanodrop, ThermoFisher Scientific, Waltham, MA). PCR amplification of immunoglobulin heavy-chain gene rearrangements from genomic DNA samples using primers in framework region 1 (FR1) and JH, as previously published38,39. DNA extraction and BCR sequencing were carried out in the Human Immunology Core Facility at the University of Pennsylvania. Experiments were performed using the Illumina 2 × 300-bp paired-end kits (Illumina MiSeq Reagent Kit v3, 600-cycle, Illumina MS-102-3003).