title: GSM8248712: IPSCs, patient, RNAseq, rep02; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNAiso Plus solution (Takara Bio Inc., Japan). 1 ug total RNA was used for the construction of sequencing libraries. Total RNA was quality and quantity were detected using Nanodrop 2000 (Thermo Fisher Scientific, MA, USA) as well as Agilent 2100 Bioanalyzer (Agilent Technologies, CA, USA). The mRNA was enriched from qualified samples using Oligo (dT) coated beads and then was fragmented using fragmentation buffer. The RNA fragments were reverse transcribed to synthesize cDNA using random primers. The library was prepared through sequentially end repair, A-Tailing, adapter ligation, size selection, and amplification.