title: GSM8028141: A375_ATACseq_HMGB2_EV1; Homo sapiens; ATAC-seq
library_construction_protocol: Live cell samples were thawed, washed, and treated with DNAse I to remove genomic DNA contamination. Live cell samples were quantified and assessed for viability using a Countess Automated Cell Counter. After cell lysis and cytosol removal, nuclei were treated with Tn5 enzyme for 30 minutes at 37°C and purified with Minelute PCR Purification Kit to produce tagmented DNA samples. Tagmented DNA was barcoded with Nextera Index Kit v2 and amplified via PCR prior to a SPRI Bead cleanup to yield purified DNA libraries.