title: 16S rRNA Gene Amplicons Analysis of Nasal Swabs from Beef Calves in Colorado: Comparison Between Apparently Healthy and Bovine Respiratory Disease Diagnosed Calves
design: The extracted DNA from 20 known bacterial strains was utilized to construct the 16S rRNA gene library (V4 region) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Sequencing of the 16S rRNA gene amplicons was performed using the Illumina MiSeq platform (2x250, paired-end).