title: GSM6604070: H3K4me3.shNC.2; Mus musculus; ChIP-Seq
library_construction_protocol: Chromatin was eluted from the antibody/protein G beads by incubating at 65 °C for 30 minutes with gentle vortex. To reverse cross-links, 6 uL of 5 M NaCl and 2 uL of Proteinase K were added into the cleared supernatant which contains eluted chromatin and incubated at 65 °C for 2 hours. And DNA from all samples was purified using spin columns and stored at -20 °C for qPCR and sequencing. 2.5ng of ChIP DNA was used to prepare libraries for Next Generation Sequencing using the NEBNext Ultra II DNA kit (New England Biolabs, Ipswich, MA), according to manufacturer's direction