title: 16S rRNA metagenome: unaffected peat, Smith MB, replicate 2
design: DNA was extracted from peat by DNeasy PowerSoil kit (Qiagen, Germany). V3-V4 regions of 16S rRNA gene were amplified with 515F/806R primers. with Phusion High-Fidelity PCR Master Mix (New England, Biolabs). Libraries were prepared using NEBNext Ultra DNA Library Pre Kit for Illumina