title: GSM7791793: AAA_RNAseq rep2; Mus musculus; RNA-Seq
library_construction_protocol: mRNA-seq was conducted using CD4+ T cells. Activated CD4+ T cells that had been transduced with wild-type or mutant FoxP3 were sorted based on Thy1.1 reporter expression. For each sample, 1X106 cells were sorted and subjected to total RNA extraction using TRIzol reagent and Direct-zol RNA Miniprep Kits. Quality control and the construction of mRNA-seq libraries were undertaken by Novogene Co. Ltd.. The NEB Next Ultra II kit and the non-directional mRNA approach with the polyA pipeline were used. The libraries were subsequently sequenced using the Illumina NovaSeq 6000 instrument, generating paired-end reads with a length of 2X150 bp, resulting in ~30 M reads per sample.