title: GSM7791816: FoxP3 pulldown_nucleosome rep1; Mus musculus; OTHER
library_construction_protocol: Mouse EL4 cells were lysed using a hypotonic buffer (20mM Bis-Tris pH 7.5, 0.05% NP-40, 1.5mM MgCl2, 10mM KCL, 5mM EDTA, 1Xmammalian protease inhibitor) and the nuclear fraction was isolated by spinning down at 4˚ and 2,500 RPM for 10 mins. The isolated nuclear fraction was then digested with Micrococcal Nuclease (Thermo Scientific, #88216) for 1 hour at 4℃ to fragment the chromatin into individual nucleosomes. The libraries were made by NEBNext® Ultra™ II DNA Library Prep Kit (Illumina) according to the manufacturer's instructions and submitted to Novogene for NGS with PE150.