title: GSM7777862: senescent, shMETTL3, KAS-seq, IP; Homo sapiens; OTHER
library_construction_protocol: IMR90 cells were incubated in media with 5 mM N3-kethoxal for 10 min at 37°C. Cells were pelleted and labelled genomic DNA (gDNA) was extracted by using Quick gDNA mini plus kit. DNA was eluted with 25 mM K3BO3 (pH 7.0) and went through click reaction with 20 mM DBCO-PEG4-biotin in PBS solution. Biotinylated gDNA was fragmented by Tn5 transposase at 37°C for 30 min. Fragmented DNA was purified and eluted by DNA Clean & Concentrator-5 kit and was pulled down by streptavidin C1 beads. DNA-conjugated beads and corresponding inputs were used for library construction by using index primers and purified by two-sided size selection with Ampure XP beads.