title: RNA-seq of human CD4: control siRNA
design: Total RNA was poly-A selected and library construction performed with random primers using the Illumina TruSeq Stranded mRNA kit. RNA was fragmented under high temperature with divalent cations. The fragmented RNA was reverse transcribed with random primers. The Illumina library construction was performed with automated liquid handling on the Sciclone instrument (Perkin Elmer). Sequencing on the NovaSeq-6000 was using v. 1.5 chemistry with paired 100-bp reads, reaching 40 milllion reads per library.