title: GSM7778677: Spinal 4_spatial; Homo sapiens; OTHER
library_construction_protocol: Samples were immediately cleaned from the blood and connective tissue and frozen on dry ice. Spinal cord tissues were cut transversely, parts were embedded in optimal cutting temperature (OCT) compound for spatial transcriptomics. Cryosections were cut at 10-μm thickness and mounted onto the GEX arrays. Sections were placed on a Thermocycler Adaptor with the active surface facing up and were incubated at 37°C for 1 min. Then, sections were fixed with methyl alcohol at -20°C for 30 min, followed by staining with hematoxylin and eosin (H&E) (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702). Visum spatial libraries were constructed using Visum spatial Library construction kit (10x Genomics, PN-1000184) according to the manufacture's introduction.