title: bio_28-SP28
design: Soil DNA was extracted from 0.25 g soil using the PowerSoil DNA isolation kit (MoBio, Carlsbad, CA) following the manufacturers instructions. Fungal ITS region was amplified using the PacBio SMRT Sequel platform with the primers ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS4 (TCCTCCGCTTATTGATATGC) targeting the entire ITS region 51.