title: GSM7818508: PD3; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs were separated using SepMate within 30 minutes after being collected.  The insert was added with density gradient medium, and the SepMate tube was filled with an identical amount of blood samples that had been diluted with phosphate buffer saline (PBS) and 2 percent fetal bovine serum (FBS). Then, tubes were then centrifuged at room temperature with 1200 × g for 10 minutes. The top layers were transferred into fresh tubes and twice washed with PBS including 2% FBS. The tubes underwent 120 × g for 10 minutes centrifugation at room temperature. Libraries for scRNA-seq were generated using the chromium controller according to the 10× chromium Next GEM Single Cell 3' v3.1 protocol. After mixing cell suspension and loading on Single Cell 3' v3.1 Gel Beads, cDNA library was amplified and sequenced on Illumina HiSeq platform.