title: Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (Essen) 2022
design: Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (Essen) 2022
library_construction_protocol: Naked mole rats (Heterocephalus glaber) or mice (Mus musculus, C56BL6/J) were culled by neck decapitation and full-thickness 4 and 1 mm2 dorsal skin biopsies, respectively, were immediately collected. Naked mole rats (Heterocephalus glaber) or mice (Mus musculus, C56BL6/J) were culled by neck decapitation and full-thickness 4 and 1 mm2 dorsal skin biopsies, respectively, were immediately collected. Single cell cDNA libraries were prepared using the 10X Genomics Chromium Single Cell 3' kit (User Guide Version 3) according to the manufacture's instruction. Quantification of the libraries was carried out using the Qubit dsDNA HS Assay Kit (Life Technologies), and cDNA integrity was assessed using D1000 ScreenTapes (Agilent Technologies).