title: GSM6805156: Upf2fl/fl NPCs, AAV9-Cre treatment biol rep#8, 72 hours [UC_8]; Mus musculus; RNA-Seq
library_construction_protocol: 72 hours after the infection, media was removed from the well and 1 ml TRIzol was added to each well to extract total RNA following the manufacturer protocol. DNase treatment was performed at 37 ºC for 35 min to degrade residue genomic DNA after TRIzol RNA isolation with 1 unit of Turbo DNase per 10 µg total RNA. Following the DNase treatment, RNA was purified again using phenol-chloroform pH 4.5. RNA concentrations were measured using a Nanodrop 2000c. All samples passed the QC test in the bioanalyzer analysis with a RIN of at least 9.0. RNA-Seq libraries were constructed using Illumina TruSeq Stranded Total RNA LT Sample Prep kits according to the manufacturer protocol.