title: GSM6648081: AJ, American, Rep3, RNA; Mus musculus; RNA-Seq
library_construction_protocol: Genomic DNA was extracted from liver samples using the Qiagen DNEasy kit, with an additional RNase incubation step (50 μg/sample, 30 minutes) prior to column application to remove RNA. RNA was isolated from mouse liver using a Maxwell 16 LEV simplyRNA kit. Whole genome bisulfite sequencing single indexed libraries were generated using NEBNext Ultra DNA library Prep kit for Illumina. Input gDNA was fragmented by Covaris S220 or LE220 Focused-ultrasonicator to average insert size of 350bp. Size selection was performed using AMPure XP beads and insert sizes of 300-400bp was isolated. Samples were bisulfite converted after size selection using EZ DNA Methylation-Gold Kit or EZ DNA Methylation-Lightning Kit. cDNA was generated using a Transcriptor First Strand cDNA Synthesis Kit (Roche). Strand specific mRNA libraries were generated using the TruSeq Stranded mRNA protocol.