title: GSM7329066: Ct-1; Mus musculus; RNA-Seq
library_construction_protocol: A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. RNA degradation and contamination was detected by 1% agarose gels; RNA purity was checked using the Qubit® 3.0 Flurometer(Life Technologies, CA, USA); RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample.