title: Illumina HiSeq 3000 paired end sequencing; Effect of high-dose epinephrine on myocardial gene set expression
design: Effect of high-dose epinephrine on myocardial gene set expression
library_construction_protocol: Male and female mice were injected with NaCl vs epinephrine with harvest of left ventricular tissue 2h later. Total RNA was isolated from homogenized left ventricular tissue using TRIzol (Invitrogen). Strand-specific TruSeq mRNA libraries were prepared at the Cologne Center for Genomics (CCG), Cologne, Germany (ribo-zero, 2x75nt, >30M fragments).