title: GSM8196647: wild type, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: To obtain single cell suspension, esophagus tissues from p63-KO and littermate control at E18.5 were dissected, washed with PBS and then the muscle layer was removed using fine forceps. The tissues were treated with dispase (Corning 354235) for 5 min at room temperature. DMEM containing 10% FBS was added to stop digestion and then the epithelial layers were treated with 0.1% Trypsin for 10 min at 37°C. DMEM containing 10% FBS was added to stop digestion and then the cells were filtered through 40-μm strainers, and then centrifuged at 300g for 5 min to obtain cell pellet. Single Cell 3' V2 chemistry kit (10x Genomics) was used to produce single-cell barcoded droplets and libraries according to the manufacturer's protocol.