title: 16S rRNA Gene Amplicons Analysis of Nasal Swabs from Beef Calves in Colorado: Comparison Between Apparently Healthy and Bovine Respiratory Disease Diagnosed Calves
design: PCR-grade water was employed in the construction of the 16S rRNA gene library (V4 region) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Subsequently, sequencing of the 16S rRNA gene amplicons was conducted using the Illumina MiSeq platform (2x250, paired-end).