title: GSM7329504: ATXN1-82Q Tg Cerebellum 12 Weeks Old Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Cerebella were dissected out, flash frozen, and kept at -80°C until further processing. Anatomical location was verified under a dissection microscope. The same individual conducted all tissue extractions to eliminate variability in sample collection. Total RNA was extracted from frozen mouse cerebellum using the Qiagen RNeasy Mini Kit (Qiagen, 74106) per the manufacturer's protocol. RNA was sent to the Yale Center for Genomic Analysis for library preparation and sequencing. Libraries were prepared using the Illumina TruSeq Stranded mRNA kit following manufacturer's protocol. Briefly, this kit selects for transcripts containing a polyA tail and uses dUTPs to produce a reverse stranded library. Sequencing was conducted using standard Illumina protocols.