title: GSM6744921: WT1_CTCF_IP_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Decrosslinking from beads was performed in a 1% SDS solution containing Tris-HCl pH8 and 10 mM EDTA for 2 hours at 69°C with agitation. Decrosslinking of the DNA-protein complex was performed by adding Proteinase K (20 ng/mL) for 2 hours at 62°C with agitation. DNA was then purified using ExpinTM PCR SV (103-102, GeneAll®). ChIP-Seq libraries were generated according to Illumina's instructions and single-end (SE) sequencing was performed on the Illumina Hi-Seq 2500 system at the Technion Genome Center (Haifa, Israel)