title: GSM7510874: Supraspinatus day 14 Bursa removed, rep 6; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rats were sacrificed at 3- or 14-days post-operation. Bursa and supraspinatus tendon were dissected and stored in AllProtect reagent for 24 hours at room temperature before transfer to -80deg storage until further processing steps. Before RNA isolation, AllProtect was removed via centrifugation in nested Eppendorf tubes (inner tube punctured with hole to drain off AllProtect). Tissues were then snap frozen and homogenized using a ball mill homogenizer. RNA extraction QIAzol lysis reagent and Maxtract high density phase lock gel tubes. RNA purification was confucted using the RNeasy Mini Kit spin columns. RNA concentraiton was then quantified and checked for quality using the Bioanalyzer (Agilent) prior to sequencing. used poly-A pull-down to enrich mRNAs from total RNA samples, then proceeded with library construction using Illumina TruSeq chemistry