title: GSM8027995: Vector-3; Homo sapiens; RNA-Seq
library_construction_protocol: Nucleic acid extraction was accomplished using the (Tengen DP411, CLB + Adderall RN40 CTAB + Adderall RN40, Tengen DP762-T1C) kit/TRIzol reagent. The first step is to purify and break the mRNA, the second step is to synthesize the first strand of cDNA, the third step is to synthesize the second strand of cDNA and purify the second strand product, the fourth step is to repair the ends and add A to the 3'-ends, the fifth step is to ligate the junctions, the sixth step is to purify the ligated product and select the fragment, the seventh step is to amplify the ligated product, the eighth step is to purify the PCR product, and the final step is to perform a quality check on the library by using the Qsep-400 method.