title: Full-length genome sequence of porcine epidemic diarrhea virus strain DY2020
design: Specific primers for pedv amplification were designed, and the whole genome of PEDV DY2020 strain was amplified by conventional reverse transcription polymerase chain reaction (RT-PCR). To put it simply, the tissue RNA of DY2020 infected cells is extracted and reversed into cDNA. The RT-PCR amplification protocol consisted of an initial denaturation step at 98C for 30 s, followed by 30 cycles at 98C for 10 s, 55C for 30 s, 72C for 90 s, and finally an extension of 10 min at 72C. After purification, the RT-PCR products of each gene fragment were sequenced three times, and finally the whole genome sequence was spliced through the RDP4 website.