title: GSM7883911: lymph_CD4,replicate1; Sus scrofa domesticus; RNA-Seq
library_construction_protocol: Total RNA was extracted from each T cell population using a RNeasy Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol with three biological replicates. RNA purity and concentration were determined using a NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). The cDNA library construction and sequencing were carried out in accordance with the Illumina standard protocol by Beijing Novogene Bioinformatics Technology Company. The library quality was evaluated using an Agilent Bioanalyzer 2100 system. The cDNA library was sequenced on an Illumina Hiseq platform, and 150bp paired-end reads were generated.