title: Nanopore DNA sequencing of Equus ferus przewalskii: adult female whole blood
design: gDNA was extracted with a Qiagen MagAttract kit, library prepped with the ONT LSK-114 kit, and sequenced on a P2 Solo instrument and single PromethION R10.4.1 flow cell over 96 hours with washing and reloading every 24 hours. Basecalling was performed with Dorado v0.4.3, "super accuracy" model dna_r10.4.1_e8.2_400bps_sup@v4.2.0, detection of modified bases with option "--modified-bases 5mC_5hmC."