title: WGS of bacteria from borehole groundwater in and around the north-West University in the North West Province, South Africa
design: Four MDR isolates from groundwater (boreholes) from South Africa  were selected for WGS. Whole-genome paired-end libraries were generated using the NEBNext kit (BioLabs, England) library kit according to the NEBNext for Illumina protocol. Lastly, the libraries are cleaned and pooled. Sequencing then takes place by loading the 4 nM pooled library onto the Illumina MiSeq platform using the MiSeq Reagent Kit V3.