title: Illumina HiSeq 4000 sequencing; ChIP-seq of HCC1806 cells (H2Bub1, H3K79me3, H3K27ac), treated with siControl or siRNF40 for 48 hours
design: ChIP-seq of HCC1806 cells (H2Bub1, H3K79me3, H3K27ac), treated with siControl or siRNF40 for 48 hours
library_construction_protocol: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 C and 5% CO2 siRNA transfections were performed using Lipofectamine RNAiMAX(Invitrogen) according to the manufacturer's instructions Chromatin immunoprecipitation was performed 48 hrs after transfection using antibodies against H2Bub1 (Cat. No. 5546 S, Cell Signaling Technology), H3K79me3 (Cat. No. C15410068, Diagenode), H3K27ac (Cat. No. C15410196, Diagenode). Next-generation sequencing library was prepared using the KAPA Hyper Prep Kit (KR0961–v6.17)  according to the manufacturer's instructions and samples were sequenced (single-end 50 bp).