title: GSM6918874: Camellia_BoneMarrow-HSC_LL731_P2_RNA; Mus musculus; RNA-Seq
library_construction_protocol: Individual cells were directly sorted into 96-well plates containing methyltransferase reaction mixture and incubated for 30 minutes at 37°C. Dynabeads Myone Carboxylic Acid (Invitrogen, 65011) was used to remove nuclei. Assisted with magnetic stand, the binding buffer containing cytoplasmic mRNA was transferred to a separate 96-well plate. The cytoplasmic mRNA was processed with scSTRT-seq with minor modifications to profile the whole transcriptome