title: GSM8196784: liver，HFrD induced NAFLD model，rep2; Mus musculus; miRNA-Seq
library_construction_protocol: According to the manufacturer's instructions, liver total RNA in HFrD mice and control mice was extracted from TRIzol (Invitrogen, CA, USA). Total RNA (1 μg) was used for removing the rRNAs using Ribo-Zero rRNA Removal Kits (Illumina, San Diego, CA, USA) following the manufacturer's instructions. RNA libraries were constructed by using rRNA-depleted RNAs with TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). 10 pM libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 150 cycles on Illumina NovaSeq 6000 Sequencer according to the manufacturer's instructions.