title: Metagenomic sequencing of wild Cervus elaphus: feces
design: METAGENOMIC. Fecal DNA was extracted using the PowerFecal DNA Isolation Kit (MOBIO, USA) in accordance with the manufacturers instructions. The genomic DNA was randomly sheared into short fragments. The obtained fragments were end repaired, A-tailed and further ligated with Illumina adapter. The fragments with adapters were PCR amplified, size selected, and purified. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina Novaseq 6000 platform, according to effective library concentration and data amount required.