title: Supplementation of a Yeast Fermentate on Broiler Growth, Processing Characteristics, and Carcass Rinsate Microbiota in Commercial Diets
design: Carcass rinsates were centrifuged at 5,000  g for 30 minutes. The supernatants were decanted and resuspended in 1 mL of 1  PBS, and stored at -80 C until DNA could be extracted. Genomic DNA was extracted using a DNeasy PowerSoil Pro Kit (Qiagen, Valencia, CA, United States) following the manufacturer protocol with modifications:  Prior to bead-beating, samples were incubated at 65 C for 10 min; and the final elution step used 50 L.