title: GSM7790983: DM-sEV DM1; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Total RNAs were extracted from sEV purified from 2 ml of rat plasma using TRIZOL (Invitrogen, Carlsbad, CA) per the manufacturer's protocol. Both 3' and 5' adaptors were added to each end, respectively, followed by reverse transcription and PCR amplification. The PCR products derived from the 18- to 30- nucleotide RNA molecules were purified by electrophoresis and sequenced using the Illumina HiSeq 2500 platform.