title: Illumina HiSeq 1500 paired end sequencing; Characterization of mutations in West Nile virus NS4B protein identifies proline residue 54 as a determinant of attenuation
design: Characterization of mutations in West Nile virus NS4B protein identifies proline residue 54 as a determinant of attenuation
library_construction_protocol: Virus was collected from the supernatant of transfected or infected Vero cells Vero cells at 80% confluency were infected at MOI of 0.1 RNA was extracted using the Qiagen QIAamp Viral RNA kit  cDNA libraries were prepared using random hexamer RT-PCR primers