title: GSM8027763: drw1-ATAC-1; Oryza sativa; ATAC-seq
library_construction_protocol: About 2,0000 nuclei were harvested and was used for library construction. ~2,0000 nuclei were collected in each 20 μl Tn5 transposase integration reaction for 30 min at 37°C. Subsequently, DNA fragments were purified using VAHTS DNA Clean Beads (Vazyme, N411), eluted in 15 μl elution buffer. Subsequently, the entirety of each sample was amplified applying NEB Next Master Mix and barcode primers for 15 PCR cycles. These amplified ATAC-seq libraries were purified and sequenced on an Illumina Novaseq6000 platform with150-bp paired-end reads.