title: GSM7225273: case33, PER; Felis catus; RNA-Seq
library_construction_protocol: Tissue samples were homogenized in Trizol and RNA was extracted from the aqueous phase using the Quick-RNA Prep Kit (Zymo Research). Samples were treated with DNase on an RNA Clean & Concentrator column (Zymo Research) if high molecular weight material was observed on a Fragment Analyzer trace. Ribosomal RNA was depleted with the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat; New England Biolabs) using 500 ng input total RNA and RNA-seq libraries were generated with the NEBNext Ultra II Directional library prep kit (New England Biolabs).