title: GSM7882546: CA2_Control_scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: The tissues were washed in ice-cold DMEM, dissociated, and homogenized using a tissue dissociation kit (MiltenyiBiotec, Köln, Germany) according to the manufacturer's instructions. Erythrocyte lysis solutions (MiltenyiBiotec) were used to remove erythrocytes, and cell counts and viability were estimated with a fluorescent cell analyser (Rigel S2, Countstar, China) and AO/PI reagent. Finally, fresh cells were washed twice in DMEM and resuspended at 1 × 10^6 cells/ml in phosphate buffered solution (PBS) and 0.04% bovine serum albumin for single-cell sequencing. To generate single-cell gel bead emulsions, single-cell suspensions of CA tissues were immediately injected into Chromium controllers (10×Genomics) with a target of 9,000 cells. Based on the principles of microwell technology, the Microwell & Magnetic Beads kit (SeekOne) was used to capture single cells and for transcriptome library construction. Nucleic acid-modified beads were used to molecularly label mRNAs of different cellular origins, resulting in the construction of a high-throughput single-cell transcriptome library compatible with the sequencer (Illumina)