title: 12-letter DNA (Val20) - BSc/XK
design: Additional pooled sequencing run of the Val20 validation set for 12-letter DNA model (ATGCBSPZXKJV) used to obtain higher coverage of B:Sc;Xt:Kn sequences. XNA tailing and XNA ligation was used to build sequences with each of the XNA base pairs (B:Sc;Xt:Kn). Ligation reactions for each base pair were carried out independently then pooled for sequencing reaction. Sequenced on a MinION r9.4.1 flowcell using SQK-LSK110. Data collected in this run forms was used for benchmarking recall of the 12-letter DNA model (ATGCBSPZXKJV) in a sequence context outside of what was used to build the model.