title: Amplicon sequencing of soybean rhizosphere soil
design: PCRs for amplicon sequencing were conducted on soybean rhizosphere DNA with 16S rRNA primers (from Caporaso et  al., 2012) and rpoB (targetting alphaproteobacteria primers designed in (Wardell G. thesis 2024) from three differnt farms in the UK (NFF, HF, TF) with 5 plant replicates. Amplicons were cleaned, indexed and sequenced at the Centre for Genomics Research (University of Liverpool), on Illumina Miseq (2x250 bp). Raw Fastq files were trimmed for the presence of Illumina adapter sequences using Cutadapt v1.2.1. Singlet and paired reads are provided.