title: GSM7147845: NB32; Homo sapiens; RNA-Seq
library_construction_protocol: TRIzol reagent, Direct-Zol RNA kit, Samples with RNA Integrity Numbers (RIN) more than 6.5 were selected TruSeq RNA library Prep Kit v2 (Illumina), Poly A selection with oligo dTs conjugated to paramagnetic beads. Then first and second strands cDNA libraries were constructed from 100 ng of extracted RNA using the TruSeq RNA Library Prep kit v2 (Illumina) as per kit's instruction. T-A ligation was added to the blunted and A-tailed cDNA, before doing 12 cycles of PCR to incorporate illumine adapters containing multiplexing barcodes.