title: GSM7777746: aal1OE_CLS_d6_biolRep1; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: RNA was extracted with the Qiagen RNeasy Mini Kit. RNA was extracted with RNeasy spin columns and digested with DNase I (Qiagen) for 30 minutes at room temperature followed by a column cleanup with RNeasy spin columns. The rRNA was removed using the Ribo-Zero rRNA removal kit (Illumina) as per recommendations. RNA quality was assessed in an Agilent 2100 Bioanalyzer and strand-specific cDNA libraries were made with NEXTflex™ Rapid Directional qRNA-Seq™ Kit (PerkinElmer, formerly BiooScientific) with molecular indexing. Libraries were quantified in a Qubit, pooled and 4 nM of the pooled library was sequenced in an Illumina HiSeq2500 sequencer, 50 bp paired-end reads in two lanes.