title: GSM7913123: LIB5455516_SAM24414655_S9_L001; Homo sapiens; RNA-Seq
library_construction_protocol: Cells from each sample were washed and re-suspended in 180µl of PBS+1%BSA and transferred to a round-bottom 96-well plate. Then 20µl of each anchor-barcode mix was added to the suspension for a final concentration of 2µM. After the incubation for 15 minutes at room temperature, 20µl of co-anchor was added to each sample (final concentration of 2µM) and incubate for another 15 minutes at RT. Cells were then washed by 500µl of cold PBS+1% BSA twice before pooling together. The library construction was done following the manufacturer's manual with a few modifications to accommodate the MULTI-seq protocol: (1) during the cDNA amplification 1µl of the following primer (10µM) was added to the master mix, 5'-CTTGGCACCCGAGAATTCC-3'; (2) the supernatant from the 0.6X SPRI beads cleanup for cDNA was kept and further cleaned up by using 2.0X SPRI beads; (3) the CMO libraries were made by performing 8 cycles of the indexing PCR using the supernatant. The transcriptome and CMO libraries were then sequenced by using either the Illumina NovaSeq or NextSeq machines