title: GSM6604167: set101.ChIP-seq.TOP3B-KO.starvation.RNAPII-ser2p.rep3.bw; Homo sapiens; ChIP-Seq
library_construction_protocol: Different sequencing types have different protocols, please follow the protocols in the "Materials and Methods" part in the article. Briefly, for RNA-seq, total RNAs were extracted with TRizol. Then 10µg RNA were used to purify mRNAs by oligo dT beads. The mRNAs were used to generate library for Illumina sequencing.  For ChIP-seq, the starved or no treatment cells were crosslinked by formaldehyde (final 1%). Then the chromatins were sonicated by Bioruptor before pulling down by antibodies (TDRD3, RNAPII or RNAPII-ser2p). The DNAs were extracted from the immunoprecipitated chromatins  and were used for library generation. Finally, the libraries were sent for Illumina sequencing. For RNA-seq and ChIP-seq, library construction protocol can be found here (Y. Joo et al., Topoisomerase 3beta knockout mice show transcriptional and behavioural impairments associated with neurogenesis and synaptic plasticity. Nat Commun 11, 3143 (2020).).