title: 16S-seq of shrimp microbiota: Stress at T12-07
design: V4 region of the bacterial 16S rRNA gene was amplified by PCR, using a 515F-806R barcoded fusion primer set (515F: GTGYCAGCMGCCGCGGTAA; 806R: GGACTACNVGGGTWTCTAAT). PCR was performed with the gDNA from shrimp stomach and involved the following steps: initial denaturing at 95 XC for 3 min; 28 cycles of 95 XC for 30 sec, 55 XC for 40 sec, 72 XC for 50 sec; final extension at 72 XC for 5 min. The size-selected and pooled library with equal DNA concentration per sample was used for 2 x 300 bp paired-end sequencing. High-throughput sequencing was performed on the Illumina Miseq platform. Primer sequences were trimmed from the sample reads and the trimmed sequences are deposited.