title: GSM7147967: ChIP_p300_gastruloids_1; Mus musculus; ChIP-Seq
library_construction_protocol: Ttrypsinized gastruloids were crosslinked in solution A with 2mM disuccinimidyl glutarate (Thermo Fisher Scientific #20593) for 25 min, followed by 1% formaldehyde methanol-free (Thermo Fisher Scientific, #28906) for 20 min. Chromatin extracts were sonicated for 2-4 cycles of 30 sec on, 30 sec off using a Diagenode Bioruptor Pico. For each ChIP, 4µg of V5 Tag antibody (Thermo Fisher Scientific, #R960CUS) or 4µg of HA antibody (a mix of 2µg from Roche 11867423001 and 2µg from Invitrogen 71–5500) was pre-conjugated to 40ul of Protein A/G magnetic beads (Invitrogen). Immunoprecipitated DNA was processed for library preparation using the Kapa HyperPrep Kit (Kapa Biosystems) and barcoded with NEXTflex DNA barcode.