title: GSM7777758: Adult_DM_PV, replicate 1, scRNAseq; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Drosophila proventriculus tissues were dissected in cold PBS. Samples were dissociated with a combined mechanical and enzymatic (8-10mg/ml Gibco Collagenase, Type IV, powder, Cat#17104019) dissociation. Samples were filtered (BD 20um syringe, Cat#340595) and washed. RNA extraction and library construction were performed using the 10X Genomics products including the Chromium Next GEM Single Cell 3' Kit v3.1. (PN1000128), the Chromium Next GEM Chip G Single Cell Kit (PN1000127), and the Single Index Kit T Set A (PN1000213), and following the manufacturer's protocols (CG000204 Rev D). See the method session for more details. Library was constructed using the Chromium Next GEM Single Cell 3' Kit v3.1. (PN1000128), the Chromium Next GEM Chip G Single Cell Kit (PN-1000127), and the Single Index Kit T Set A (PN1000213), and following the manufacturer's protocols (CG000204 Rev D).