title: GSM6705979: aCD_hMES_2 (RNAseq); Homo sapiens; ssRNA-seq
library_construction_protocol: Total RNA was extracted from from ASCs (100,000 cells/well) using TRIzol reagent (Roche, Basel, Switzerland). and further purified/DNase treated with the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA concentration was determined by absorbance at 260 nm, and purity was estimated with a Nanodrop spectrophotometer (Nanodrop Technologies Inc., Wilmington, DE). Integrity was assed with Bioanalyzer Nano6000 assy with RIN values above 7.0. Libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina) according to the manufacturer's protocol adapted with beads on a Sciclene NGS liquid handling robot (Calliper, PerkinElmer) with 200 ng of total RNA used as input.Final libraries were qualified using Bioanalyzer DNA 1000  (Agilent, Santa Clara, CA) and quantified by qPCR using the KAPA Library Quantification Kit KK4835 (Roche, Basel, Switzerland) prior to sequencing. Libraries were sequenced 1 x 51+8+8 bp to at least 24 million reads per sample on the Illumina HiSeq2500 platform with v4 SBS reagents