title: GSM8249261: UOK109 H3K27ac input_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: cells were fixed with 1% formaldehyde for 5 minutes, After quenching and cell lysis, chromatin was sonicated using a E220 covaris ChIP libraries were prepared using using NEBNext® Ultra™ II DNA Library Prep Kit complemented with NEB DNA Barcodes.1 ng of DNA was used as starting material for input and ChIP samples. Libraries were amplified using 15 cycles on the thermocycler.Post amplification libraries were size selected at 200-400bp in length using AMPure XP beads from Beckman Coulter. Libraries were validated using the High sensitivity DNA Kit.