title: GSM7735327: Wilding_2 sample from cross B3; Anopheles gambiae; RNA-Seq
library_construction_protocol: RNA was extracted from pools of ten female F1 progeny 3-5 days after eclosion using RNAqueousTM-4PCR Total RNA Isolation Kit (Invitrogen) and standard protocol. RNA was quantified using Qubit® 2.0 Fluorometer. The total RNA libraries were prepared in accordance with the Illumina TruSeq RNA sample preparation v2 guide (Part # 15026495, rev.D, September 2012) for Illumina Paired-End Indexed Sequencing.