title: GSM8025749: Con1; Homo sapiens; RNA-Seq
library_construction_protocol: Follicular fluid from POI patients and control group patients were collected. Exosomes from follicular fluid were extracted by ultracentfugation method, total RNA from exosomes was extracted by trizol method, and sRNA of 18-30nt was screened for high throughput sequencing The library construction process mainly includes the following steps: extraction of total RNA or purified sRNA fragments of the sample, connection of connector successively, reverse transcription into cDNA, and then PCR amplification. Then the target fragment library was recovered by cutting glue, and the qualified library was sequenced.