title: RNA-Seq of tomato: mycorrhizal leaf
design: For RNA sequencing, total RNA was extracted from root and leaf samples of two biological replicates from each treatment using EZNA Plant RNA Kit (Omega Bio-Tek, Norcross, Georgia, USA). Samples (100 mg) were processed during isolation following the manufacturers protocol. After isolation, RNA integrity was examined with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA). Libraries were sequenced with a final output of single-end reads.