title: GSM6746173: ME15-E5M1L; Mus musculus; RNA-Seq
library_construction_protocol: Embryonic mouse molars were dissected from jaws on embryonic day E15 (E0 being the vaginal plug appearance day). Minimal amount of surrounding tissue was left around the tooth germ, at the same time making sure that the tooth was not damaged in the process. The tissue was immediately stored in RNAlater (Qiagen, Düsseldorf, Germany) at −80°C for RNAseq. For RNAseq, each tooth was handled individually. Samples were homogenized using Precellys 24 homogenizer (Bertin instruments). RNA was extracted using guanidium thiocyanate-phenol-chloroform method and purified using RNeasy Plus micro kit (Qiagen GmbH) following manufacturer's protocol. The purity of RNA was measured using Nanodrop microvolume spectrophotometer (Thermo Fisher Scientific). The complementary DNA (cDNA) libraries were prepared using Ovation Mouse RNAseq System.