title: GSM7777850: ∆topAPZntopA[pLSROMtopAWT] strain, SM medium, +NOV, rep1; Streptococcus pneumoniae; RNA-Seq
library_construction_protocol: RNA (about 5 µg) was obtained from samples containing 2-4E8 cells using the RNeasy mini kit (Quiagen). RNA was depleted of ribosomal RNA using the Ribo-Zero (Bacteria) Magnetic Kit (Illumina). Libraries for RNA-Seq were prepared from 1 µg RNA using the ScriptSeq v2 RNA-Seq system (Illumina).