title: Panel capture sequencing of  Macaca mulatta: blood
design: Covaris was used to shear 1 ug genomic DNA for 70 seconds and Ampure XP beads were used for the purification. Following end repair and A-tailing, the product was added with indexed adaptors. Ampure XP beads were used to purify the product, and KAPA Hifi HotStart ready mix was used for amplification. 40-50 libraries were pooled for Agilent SureSelect Target enrichment system (agilent) following manufacturers protocol with modification. Briefly, pooled samples were hybridized to probe pool, and captured with Dyanbeads MyOne Streptavidin T1 magnetic beads. After wash, captured DNA was amplified with Phusion High Fidelity DNA polymerase (NEB). After cleanup and quantification, the diluted library was sequenced in an Illumina Novaseq 6000 Sequencer.