title: GSM6652314: FE1_Hydrazine_4hr plus 20hr washout_4mM_c; Mus musculus; RNA-Seq
library_construction_protocol: Qiazol extraction followed by DNaseI treatment 1 μg of total RNA per sample was subjected to library synthesis using the Illumina TruSeq V2 Stranded mRNA Library Prep Kit. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina dual indicess prior to 15 cycles of PCR. 1.3pM library pool was loaded on an Illumina HI OUTPUT flow cell and sequenced at 2 X75bp paired end on an Illumina NextSeq 500 instrument, approx 10 million reads per sample