title: GSM8027600: HUVEC_shScr_rep4; Homo sapiens; ATAC-seq
library_construction_protocol: Using the ATAC-seq library preparation ATAC-seq library preparation kit (ActiveMotif) HUVEC and HUAEC cells were transduced with shScr and shAmotL2 for 72 h before cells were trypsinised and counted using Trypan blue. 100,000 cells were centrifuged, and nuclei was extracted using ice-cold ATAC-lysis buffer (according to the kit protocol; Active Motif) and the pellet resuspended in the Tn5 tagmentation master mix. The transposition reaction was incubated at 37°C for 30 min at 800 rpm using a thermomixer. Tagmented DNA was purified according to the protocol's column purification step. Tagmented DNA was purified according to the protocol's column purification step, followed by PCR amplification of tagmented DNA for 10 cylces using Illumina's Nextera indexed adapters.  After the PCR reaction, libraries were purified with the SPRI magnetic beads and library quality, and concentration was assessed with Qubit bioanalyzer. 4 independent experiments were prepared for ATAC-seq for both HUVEC and HUAEC, resulting in 16 libraries in total. Libraries were then sequenced with the Illumina Hiseq platform with a 150bp paired-end repeats protocol.