title: GSM8025719: wt4 muscle fibers E17.5; Mus musculus; RNA-Seq
library_construction_protocol: Muscle fibers were directly incubated with RTL lysis buffer Nextera library preparation protocol (Nextera XT DNA Library Preparation kit, Illumina) was used for library preparation, including cDNA 'tagmentation', PCR-mediated dT adaptor addition, and library amplification The libraries were sequenced using the NextSeq 2000 P2 Reagents (100 cycles) (Illumina)