title: GSM8289645: sgCNOT3-chase 2h_rep2_SLAM12; Homo sapiens; OTHER
library_construction_protocol: Total RNA was isolated using TRIzol reagent(Thermofisher Scientific), accoding to the manufacturer's instructions. Total RNA was treated with IAA according to the SLAM-seq Kinetics Kit (Lexogen, 062.24) manufacturer's instructions.  SLAM-seq libraries were constructed using the QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD with UDI 12 nt Set B1 (Lexogen, 192.24). QuantSeq 3' mRNA-seq