title: RNA-Seq of Rhododendron anthopogon: leaf sample
design: Total RNA was isolated from frozen leaf tissues using the SpectrumTM Plant Total RNA Kit (Sigma Aldrich, USA) according to the manufacturer's instructions.  For library preparation and sequencing, RNA samples having (RIN) larger than eight were employed. cDNA was prepared using a Verso cDNA synthesis kit (Applied Biosystems, USA) from 1 g of good-quality RNA.  The resulting cDNA libraries were amplified by PCR to enrich adapter-ligated fragments, measured using a Nanodrop spectrophotometer (Thermo Scientific, USA), and quality confirmed with a Bioanalyzer (Agilent Technologies, USA).