title: GSM6745325: TL-Om1, ZFP5-meCP2, Replicate2; Homo sapiens; ATAC-seq
library_construction_protocol: A previously published OMNI ATAC-Seq protocol (Corces et al, 2017) was used for cell lysis, tagmentation, and DNA purification. The Tn5 treated DNA was amplified with 10 cycles of PCR in 50 μl reaction volumes. 1.8X AmpurXP beads purification was used for the PCR product clean-up. The libraries were validated with Agilent Bioanalyzer DNA High Sensitivity Kit, and quantified with qPCR. ATAC-seq libraries were sequenced on Illumina NovaSeq6000 with S4 Reagent v1.5 kit (Illumina, Cat 20028312) at Tgen with the sequencing length of 2x101. Real-time analysis (RTA) 3.4.4 software was used to process the image analysis.