title: GSM7328855: Rat CD4+ T-MBP cells, parenchyma NT control, rep R1; Rattus norvegicus; RNA-Seq
library_construction_protocol: The spinal cord parenchyma was dissected and homogenized by passing through a metal strainer. Lymphocytes were isolated using a 30%/64% Percoll gradient and centrifugation at 1200g, room temperature for 30min with mild acceleration and brake. Lymphocytes were collected from the interface. Cells were then FACS sorted based on fluorescent protein expression, BFP for control, GFP for KO The library was prepared from total RNA using the Collibri 3' mRNA Library Prep Kits for Illumina Systems (Thermo Fisher). Amplification of transcripts was confirmed with Agilent Bioanalyzer on DNA 1000 Chips