title: GSM7148112: P1361; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from AML patient PBMCs and AML cell lines by resuspending pellets in Trizol and purifying RNA with Direct-zol RNA Miniprep (Zymo Research # R2052). DNase treatment was done using RNase-Free DNase Set (Qiagen # 79254). RNA integrity was analyzed using the Agilent TapeStation system according to the manufacturer's protocol. RNA samples with RIN scores higher than 5.3 were sent to Novogene for mRNA library prep and sequencing. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. 100 ng total RNA isolated using trizol standard protocol (according to the manufacturer's instructions) was used for sequencing using TruSeq Stranded Total RNA Ribo Zero kit (Human/Mouse/Rat) on NovaSeq6000 S4, 150 bp paired-end.