title: GSM7818985: type IIb myofibers, untrained_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with lysis buffer prepared according to the manufacturer's instructions (SMART-Seq HT kit, Takara). cDNA was synthesis and amplified using SMART-Seq HT kit according to the manufacturer's instructions. Then the cDNA was purified with AMPure XP system (Beckman Coulter, A63880) and quantified by QuantiFluor dsDNA System (Promega, E2670). Sequencing libraries were created with the Nextera XT kit (Illumina, FC-131-1024) using 1 ng cDNA according to the manufacturer's instructions.