title: GSM7030608: Corpus_whole_mesenchyme_rep2 (scRNA-seq); Mus musculus; RNA-Seq
library_construction_protocol: Adult murine tissue was harvested by dividing the stomach into antrum and corpus. Tissue was collected after perfusing adult PdgfraH2BeGFP with cold PBS. After manual muscle removal, epithelium was removed by shaking the tissue in pre-warmed chelation buffer (10 mM EDTA, 5% FBS, X DTT, HEPES in HBSS buffer) for 20 minutes at 37°C, 250rpm. The tissue was then washed with PBS and digested for 10 minutes at 37°C in 3mg/ml collagenase II (Worthington, LS004176) in HBSS complemented with 5% FBS and HEPES. Cells were harvested by centrifugation at 300g for 5 minutes. Cells were sorted on a BD FACSAria II cell sorter, gating on DAPI negative cells to identify live cells and all DAPI negative cells were sorted. Single Cell 3′ V3.1 assay (10X Genomics).