title: GSM8152681: WT3_n3; Homo sapiens; RNA-Seq
library_construction_protocol: Medium was remove and 350µl RLTplus was add, Sample were extracted on QUIACUBE following the Rneasy Plus Micro kit protocole (Qiagen) For each sample, 100 ng of total RNA was used to perform the QuantSeq 3' mRNA-Seq Library Prep Kit for Ion Torren resulting in NGS libraries which originate from the 3' end of polyadenylated RNA. The libraries were PCR amplified and barcoded in 13 cycles and were quantified using Agilent High Sensitivity DNA kit. 100 pM of the 10 libraries were combined, emulsion PCR and enrichment were performed on the Ion OT2 system Instrument using the Ion PI Hi-Q OT2 200 kit (Thermo Fisher Scientific). Samples were loaded on an Ion PI v3 Chip and sequenced on the Ion Proton System using Ion PI Hi-Q sequencing 200 kit chemistry (200 bp read length; Thermo Fisher.