title: GSM7062954: Tregs, Lag3cKO, rep 4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the RNeasy Micro kit (Qiagen cat#74004). All subsequent sample quality assessment was performed on a Fragment Analyzer electrophoresis system (Agilent). Total RNA was normalized prior to oligo-dT capture and cDNA synthesis with SMART-Seq v4 (Takara cat#634892). The resulting cDNA was quantified using a Qubit 3.0 fluorometer (Life Technologies). Libraries were generated using the Nextera XT DNA Library Prep kit (Illumina cat#FC-131-1096). Paired End run.