title: GSM7882612: d2_MEF_O6KS2AV_input; Mus musculus; ChIP-Seq
library_construction_protocol: As described by Malik et al., Nature Comm., 2019. Reprogramming sample grown in fibroblast media were collected at day 2 after Dox induction by trypsinization and washed twice with PBS. Cells were resuspended in PBS at a density of 5 x 105/mL and then chemically crosslinked by the addition of 1% PFA for 10 min at room temperature with gentle rotation. Crosslinking was stopped by the addition of glycine to a final concentration of 125 mM for 5 min at room temperature.