title: GSM8114512: gDNA, early L3 ph-KD, 144h recovery, rep2; Drosophila melanogaster; OTHER
library_construction_protocol: Genomic DNA was isolated using QIAamp DNA Micro Kit (Qiagen) following manufacturer's instructions. For each biological replicate around 70 eye discs from female wandering larvae were dissected. Library preparation was performed by Novogene (https://en.novogene.com/). Briefly, genomic DNA was fragmented to an average size of ~350bp and then processed for DNA library preparation by following manufacturer (Illumina) paired-end protocols. Sequencing was performed using on an Illumina Novaseq 6000 (platform to generate 150bp paired-ends reads) with a coverage of at least 10x for 99% of the genome.