title: 16S rRNA seq - gut microbiota_YeastBread as substrate_t24_F3
design: Illumina library preparation was executed according to 16S Metagenomic Sequencing Library Preparation (Illumina) and adapters (Illumina Nextera XT Index Primer) were attached after seven PCR cycles. Libraries were purified using Agencourt AMPure XP (Beckman) and sequenced on an Illumina MiSeq (PE300) platform