title: GSM7063785: elfii_DSM9442_EXP_30MPa_batch01_rep3AB; Pseudothermotoga elfii DSM 9442 = NBRC 107921; RNA-Seq
library_construction_protocol: RNAs were prepared using the Maxwell 16 LEV samples kit (Promega) and ribosomal RNAs were depleted using the Illumina Ribo-zero Plus rRNA depletion kit (Illumina, San Diego, CA, USA) The sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina by following manufacturer's recommendations (NEB, Ipswich, MA, USA). Sequencing libraries were validated using a DNA Chip on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) as well as by quantitative PCR (Applied Biosystems, Carlsbad, CA, USA). The sequencing libraries were multiplexed and clustered on six lanes of a flowcell. After clustering, the flowcell was loaded on the Illumina HiSeq 2500 instrument according to manufacturer's instructions. The multiplexed libraries were sequenced using paired-end 150 cycle chemistry for the HiSeq 2500 (Illumina). Image analysis and base calling were conducted by the HiSeq Control Software (HCS) on the HiSeq 2500 instrument.