title: GSM7852966: NCI-H1299,input; Homo sapiens; ChIP-Seq
library_construction_protocol: NCI-H1299 cells were cross-linked with 1% formaldehyde (vol/vol) for 10 min at room temperature. After quenching and cell lysis,chromatin was sonicated using a E220 focused-ultrasonicator (Covaris), and immunoprecipitated with anti-H3K18la (PTM Bio, #PTM-1427RM) at 4 °C. The DNAs were extracted by phenol-chloroform method. The high-throughput DNA sequencing library was prepared using VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme Biotech). Library products of 200-500 bp in length were enriched, quantified and sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.