title: GSM6805034: WT_FL_MED1_1; Mus musculus; ChIP-Seq
library_construction_protocol: To isolate foetal liver erythroid cells, HetXHet crosses were established. Pregnant mothers were sacrificed at embryonic day 12.5. Foetuses (WT and mutant) were isolated and the foetal liver extracted. Erythroid cells were disaggregated and collected for further processing. Cells were fixed with 2mM DSG and 1% Formaldehyde, lysed, sonicated and immunoprecipitated with the antibody of interest. IP'd chromatin was tagmented using Tn5 loaded with illumina sequencing adaptors. Samples were sequenced using 40 bp paired end reads on the NextSeq platform. The strategy was to characterise the chromatin state of primary mouse-derived or the CD71+ erythroid cells (WT and mutant) derived from mESC-EB in vitro differentitation system by isolating and analysing DNA regions bound by the antibodies of interest, genome-wide.