title: GSM8196628: CAST-seq on HSCs treated with Cas9 targeting NCF1B and NCF1C, technical replicate 3; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted by column purification using QIAamp DNA Blood Mini Kit according to manufacturer's recommendation. Genomic DNA was eluted from the column in DNAse/RNAse free water. CAST-Seq was performed as previously described (Klermund et al. 2024. Mol Ther. doi: 10.1016/j.ymthe.2024.03.006.) A third PCR introduced the barcoded Illumina adapter for sequencing (NEB, NEBNext Multiplex Oligos for Illumina, Cat.# E7335, E7500, E7710, E7730). Libraries were sequenced by Azenta Life Sciences using the Illumina NovaSeq platform with a 2 x 150 bp configuration.