title: GridION sequencing; Combined analysis of genome-wide DNA methylome and transcriptome reveals the first epigenetic-based antibiotic-resistance mechanism in Acinetobacter baumannii
design: Combined analysis of genome-wide DNA methylome and transcriptome reveals the first epigenetic-based antibiotic-resistance mechanism in Acinetobacter baumannii
library_construction_protocol: Six strains of A. baumannii were studied. In detail, five field strains were isolated from blood samples (defined as strains 1, 2, 3, 4 and 6) of patients hospitalized at the University Hospital \"San Giovanni di Dio e Ruggi d'Aragona\" (Salerno, Italy). DNAs were extracted from bacterial cultures using the QIAGENÂ® Genomic DNA Handbook (QIAGEN, Hilden, Germany) Libraries were prepared starting from 600 ng of gDNA, using the rapid sequencing gDNA-barcoding kit (SQK-RBK004) according to the manufacturer's protocol