title: GSM6801708: PND5 spermatogonia 1; Rattus norvegicus; Bisulfite-Seq
library_construction_protocol: Genomic DNA  were extracted from FACS-sorted germ cells using QIAamp DNA micro kit (QIAgen, Toronto, Ontario, Canada) and  the DNeasy Blood & Tissue kit (QIAgen) for spermatozoa (Purification of DNA from sperm using the DNeasy Blood and Tissue kit; protocol 2, DY03 Aug-06,available on QIAgen website). Methyl-Seq capture libraries were prepared using the Rat SureSelectXT Methyl-Seq Target Enrichment Panels kit (Agilent) as described in the SureSelectXT Methyl-Seq target Enrichment System for Illumina Multiplexed Sequencing protocol (Agilent, Version E0, April 2018).