title: GSM8422995: L286; Homo sapiens; RNA-Seq
library_construction_protocol: Liver samples: Liver biopsies from ALD patien ts (n=2) were processed and dissociated into single cell suspensions following the protocol reported by MacParland SA et al. Organoids: Hep b-orgs (n = 4; n=1 early stage, n= 3 advanced stage) were dissociated into single cell by incubating for 30 minutes in a water bath with gently shaking at 37ºC with TrypLE Express (Gibco, 12604021) supplemented with 2mM EDTA (Life Technologies, 15575-020). Next, mild pipetting was performed to ensure organoids disaggregation. Cell suspension was filtered using a 40-um nylon mesh, pelleted, resuspended in 0.04% BSA in DPBS-/- and transported on ice until further processing. Single-cell RNA sequencing libraries were prepared using the Chromium 3' protocol