title: GSM7032009: 200d KO Muscle 1; Mus musculus; RNA-Seq
library_construction_protocol: Liver, calf muscle, and hypothalamus were removed and flash frozen in liquid nitrogen. RNA was harvested using Trizol and the Qiagen RNeasy Mini Kit (Cat# 74104). The Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina was used with 500 ng of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols