title: GSM8025636: 203714 TR Rkid TT OT | 002 | CD45+; Homo sapiens; OTHER
library_construction_protocol: For GeoMx DSP sample collections, entire slides were imaged at 20X magnification and morphologic markers were used to select ROI using organic shapes. Automatic segmentation of ROI based on KRT7pos markers were used to define AOIs, allowing to separate tumor cells (KRT7pos) from adjacent stromal cells (KRT7neg). AOIs were then exposed to 385 nm light (UV), releasing the indexing oligonucleotides which were collected with a microcapillary and deposited into a 96-well plate for subsequent processing. The indexing oligonucleotides were dried overnight and resuspended in 10 μl of DEPC-treated water. Spatial Trasncriptomics library were prepared using NanoString human Whole Transcriptome Atlas (WTA) panel probes according to manufacterer's construction.