title: GSM6803598: FN1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from harvested tumors using the Qiagen RNeasy micro kit (Qiagen; Valencia, CA) according to the manufacturer's recommendation. After quantification with Nanodrop (Thermo Scientific; Rockford, IL), RNA was stored at -80ºC. RNA-seq libraries were prepared according to SMART-seq2 protocol, with the following modifications: PCR preamplification to 10-12 cycles, two rounds beads cleanup with 1:1 ratio after cDNA synthesis, and 0.6-0.8 dual beads cleanup for Nextera XT DNA-seq library purification.