title: GSM6528449: BC-3  lytic rep-2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol and treated with DNase I. Following DNAse I treatment, RNA was recovered by extraction with phenol:chloroform:isoamyl alcohol [25:24:1 (vol/vol)] followed by ethanol precipitation. Isolated RNA was subjected to ribosomal RNA depletion using NEBNext rRNA depletion kits (Human/Mouse/Rat: NEB E6310) according to the manufacturer's instructions. The rRNA-depleted samples were used as the starting material to generate sequencing libraries using the NEBNext Ultra II directional RNA library prep Kit (NEB E7760) according to the manufacture recommendations.