title: GSM6919257: dsDouble-kd_R2; Drosophila melanogaster; ATAC-seq
library_construction_protocol: 50.000 cells were centrifuged (500 x g, 5 min, 4ºC), washed in ice-cold PBS and lysed in cold lysis buffer. Nuclei were then pelleted and used as input material for tagmentation. All the samples consist of biological triplicates. Tagmentation and library preparation was performed according to Buenrostro et al. 2015 and Yu et al. 2018. using Nextera DNA library kit (FC-121-1030). Libraries were sequenced in NextSeq500, obtaining paired-end, unstranded 37 cycles reads.