title: GSM7030066: Nod1-MO2, replicate 2; Danio rerio; RNA-Seq
library_construction_protocol: approximately 150 24hpf zebrafish embryos were dechorionated with pronase, anesthetized in 1% tricane, gently shaken at 28C for 5 mins with 0.05mg/ml liberase TM (Roche) in PBS solution with Ca2 + and Mg2+. The resulting cell suspension was filtered and stained with Sytox Red to exclude dead cells. kdrl+ cells were sorted with BD FACS Aria III. RNA was isolated and purified with RNeasy Micro Kit (Qiagen). Total RNA was assessed for quality and quantity using an Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit. Samples were then used to generate RNA sequencing libraries using NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB #E6420S/L) for Illumina.