title: GSM7601028: EPIA02DNA_Patient_OSA; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Total DNA was extracted using Qiagen RNeasy purification procedure and mRNA was purified using oligo(dT)-attached magnetic beads. The DNA was broken down into smaller fragments using sonication, resulting in fragment sizes ranging from 100 to 300 base pairs. Following that, a process called end-repair was carried out to prepare the fragments for further analysis. Methylated sequencing adapters were then added to the 3' ends of the DNA fragments. To convert the non-methylated DNA into bisulfite-converted DNA, we used the EZ DNA Methylation-Gold kit from Zymo Research in California, USA, following the instructions provided by the manufacturer.