title: GSM7818566: HCT116_rep_3_RNA_seq; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using RNeasy Plus Mini Kit (QIAGEN) following the manufacturer's instructions. Quantity and quality of RNA samples were assessed using the Nanodrop spectrophotometer (Nanodrop ND-1000, Thermo Scientific) as well as the Qubit fluorometer (Invitrogen) with the Qubit High sensitivity RNA reagents. In addition, size distribution of RNA fragments and the RNA integrity number (RIN) value were determined by using Agilent 2100 BioAnalyzer with the RNA nano chip. RNA samples for whole genome sequencing were send to Edinburgh Genomics for Library preparation and Sequence data generation. Library preparation was performed using the TruSeq stranded mRNA-seq library kit (Illumina). The libraries were then sequenced on NovaSeq with 50 paired end (PE) reads and aiming for at least 375M reads per sample.