title: GSM7225004: 16-cells embryos 5C; Bos taurus; OTHER
library_construction_protocol: Bovine oocytes and embryos from all study groups were digested with proteinase K (50 mg/mL) in M-Digestion buffer 10X (Zymo Research) for 20 minutes at 50 oC to remove the zona pellucida. Then, they were submitted to total DNA extraction using QuickDNA Microprep Kit (Zymo Research; D3020) following the manufacturer protocol. Library construction was performed using the Enzymatic Methyl-Sequencing kit from New England Biolabs (E7120S), following the manufacturer protocol. Enzymatic Methyl-Sequencing