title: FBAP2
design: The universal primers F63 (5-GCA TAT CAA TAA GCG GAG GAA AAG-3) and LR3 (5-GGT CCG TGT TTC AAG ACG G-3) were employed for amplifying the fungal D1/D2 region of the large subunit of the 28S ribosomal gene (LSU), as specified by Fell et al, (2000). PCR was conducted utilizing the HotStarTaq Plus Master Mix Kit from Qiagen, and the success of amplification was assessed by analyzing PCR products on a 2% agarose gel. The resulting PCR amplicons underwent standard paired-end DNASeq library preparation following the Illumina HiSeq 4000 manufacturer's protocol. Sequencing was performed using a 101bp paired-end module sequencing approach.