title: Illumina Nextseq of Fusarium poae: IBT 9973
design: 700ng input of gDNA samples were mechanically sheared to 300bp insert using Covaris M220 instrument (Covaris). The obtained inserts were used as a template to construct NxSeq AmpFREE Low DNA Library kit and NxSeq single Adaptors (LGC Biosearch Technologies) according to the library prep protocol. The library yield was excellent between 32-67nM using KAPA library quantification kit (Roche Diagnostics) and the LightCycler 480 Real-Time PCR instrument