title: Metatranscriptome of hydrothermal vent plume water: Cast 1, Bottle 4, Filter 1
design: Total RNA was extracted with the Macherey-Nagel NucleoMag DNA/RNA Water kit. RNA quantity was measured with the Qubit HS RNA assay and quality was checked on an Agilent Fragment Analyzer with the HS RNA kit. Illumina TruSeq Stranded Total RNA Prep kit with Ribo-Zero Plus was used to prepare libraries following manufacturer's SOP.