title: GSM8152413: U87 RNase R-2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using TriZOL and Direct-zol RNA extration columns (Zymo Research). SynCRS1-6 were spiked-in at known amounts to 2ug of RNA which was then digested with RNase R (2U/ug RNA; Epicentre) for 30mins at 37oC, then purified by Rneasy MinElute Cleanup kit (Qiagen). Stranded RNA Libraries were constructed with RNase R-treated RNA using the Tecan Universal RNA-Seq Library with Human Probe (for rRNA depletion) protocol M01508 V2.