title: eukaryotic (18S) metagenome
design: 18S rRNA gene amplicons were prepared and sequenced using 300 bp paired-end read Illumina MiSeq V3 sequencing by LGC Genomics (Berlin, Germany). Using the primer pair 1183F (AATTTGACTCAACRCGGG)/ 1443R (GRGCATCACAGACCTG)