title: 22Rv1-AUR-2
design: 13 g of genomic DNA was sheared using a Covaris S2 to a peak target size of 250 base pairs (bp). Fragmented DNA was concentrated using AMPure beads, followed by end-repair, A-base addition, ligation of the Illumina indexed adapters, and size selection on 3% Nusieve agarose gels (Lonza). Fragments between 300 to 350 bp were recovered, amplified using Illumina index primers, and purified by AMPure beads. One microgram of the library was hybridized to the Agilent SureSelect Human All Exon v.4. The targeted exon fragments were captured and enriched following the manufacturers protocol (Agilent). Paired-end whole-exome libraries were analysed by an Agilent 2100 Bioanalyzer and DNA 1000 reagents and sequenced using an Illumina Nova 6000 (Illumina).