title: GSM8196338: meninges, LCMV; Mus musculus; RNA-Seq
library_construction_protocol: After harvesting, the meninges of 10 mice from different groups were washed in ice-cold RPMI1640 and dissociated using Collagenase IV (Sigma), Collagenase II (Sigma), Collagenase I (Sigma), and DNase I (Sigma). Cell count and viability were analyzed using a fluorescence Cell Analyzer (Countstar) with AO/PI reagent after removing erythrocytes (Solarbio). The debris and dead cells were removed (Miltenyi). Finally, fresh cells were prepared for library construction. Single-cell RNA-Seq libraries were prepared using the SeekOne Digital Droplet Single Cell 5' library preparation kit (SeekGene).