title: GSM6918269: StandardExos_miRNA_biol rep 1 [Base_Exos_1_S4]; Cricetulus griseus; miRNA-Seq
library_construction_protocol: Followed protocol of NextFlex Combo-Seq mRNA/miRNA kit (PerkinElmer) Following isolation of cells, MPs, and exosomes, total RNA was isolated with the miRNeasy micro kit (Qiagen). RNA concentration was determined using the Qubit RNA High Sensitivity (HS) kit and size distribution of total RNA was determined by fragment analysis (AATI Fragment Analyzer, Agilent). The 9 standard culture sample libraries were pooled together.  The 18 osmolarity and ammonia stressed sample libraries were pooled together.