title: GSM7031908: CAGE_dhh1D_Replicate_2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: CAGE libraries were constructed from 5µg of total RNA of each strain using the nAnT-iCAGE protocol (Murata et al. 2014) by K.K. DNAFORM of Japan. Briefly, first strand cDNAs were transcribed to the 5' end of capped RNAs and attached to CAGE “barcode” tags. Each nAnT-iCAGE library used linkers with specific barcodes and was sequenced using Illumina NextSeq500 (single-end, 75-bp reads).