title: GSM7151711: kdm5[140] RG_rep4; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Trizol extraction of total RNA from dissected ring glands was performed according to manufacturer's instructions. Animals were matched by developmental stage of wandering third instar larva. n = 80 ring glands per replicate. Total RNA was submitted to Novogene for library construction and sequencing. At Novogene, polyA enrichment was performed to prepare mRNA libraries. Libraries were evaluated for quality using the Agilent Bioanalyzer 2100 system. Sequencing was performed on an Illumina NovaSeq PE150 sequencing platform (2 x 150bp) cycles.