title: Whole genome metagenome analysis of bovine mastitis;Grazing mastitis cow milk:S2
design: Library preparation was performed using QIASeq FX DNA Library Preparation protocol. Briefly, 5-50ng of Qubit quantified DNA was enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FX Enzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragments were subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, to generate sequencing library. The library was subjected to 6-11 cycles of Indexing-PCR to enrich the adapter-tagged fragments.Finally, the amplified library was purified using Sera-MagTM Select beads followed by library quality control check. Illumina-compatible sequencing library was quantified by Qubit fluorometer and its fragment size distribution was analyzed on Agilent 2200 TapeStation.