title: GSM8026832: embryo, wild type, GAF, ChIP, 0.5-1.5h, rep1; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: The embryos of indicated genotypes were harvested and subjected to cross-linking by fixation buffer (50 mM HEPES, pH 8.0, 1 mM EDTA, 0.5 mM EGTA, 100 mM NaCl and 1.8% formaldehyde).  They were cross-linked for 20 minutes and then quenched with 125 mM glycine (final concentration) for 5 minutes at room temperature.  Chromatin immunoprecipitation (ChIP) reactions were carried out in duplicate using the Pierce Magnetic ChIP Kit and the following antibodies: mouse anti-dPCIF1 (1:100), mouse anti-GAF (1:100), mouse anti-Zelda (1:100), mouse anti-HIRA (1:100), rabbit anti-RNA polymerase II-phospho S5 (1:200). Sequencing libraries were generated using the VAHTS Universal DNA Library Prep Kit for MGI.