title: Bisulfite-Seq of Ixodes scapularis: pooled males from TX S5
design: DNA was isolated from pooled ticks using Quick-DNA/RNA Microprep Plus kit. Purified DNA samples were then sequenced using the whole genome bisulfite sequencing. For Bisulfite conversion, the EZ DNA Methylation Gold Kit was utilized, and the library was prepared using Accel-NGS Methyl-Seq kit. The resulting library was sequenced on Illumina HiSeq instrument.