title: GSM7148059: MDA-MB-231 NRP-1 KO cells  (Replica #1); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted for library preparation using mRNA with poly-A tail and non-coding RNAs The RNA was fragmented for short read sequencing which was then reverse transcribed to cDNA that was ligated with adaptors at both ends. The DNA fragments were amplified using PCR, and the fragments with insert sizes between 200-400 bp were selected for paired-end sequencing, both ends of the cDNA are sequenced by the read length full method