title: Illumina HiSeq 4000 sequencing; RNA-seq of human breast cancer cell lines MCF7 and CAMA1 treated with Fulvestrant, AZD9496 and AZD9833/camizestrant, with and without estradiol
design: RNA-seq of human breast cancer cell lines MCF7 and CAMA1 treated with Fulvestrant, AZD9496 and AZD9833/camizestrant, with and without estradiol
library_construction_protocol: Samples were harvested in 1 mL of QIAzol lysis buffer and snap frozen. RNA was extracted using RNeasy 96 QIAcube HT total RNA Cell with DNAse treatment, and samples randomized over 96-well plates. RNA was quantified using the Qubit RNA BR kit (Q10213 Invitrogen), according to the manufacturerâ€™s instructions. The Illumina Truseq stranded mRNA library was generated by the Cancer Research UK Cambridge Institute Genomic core facility