title: NextSeq 550 paired end sequencing; Ie mutant mouse RNA-seq sample Mut_5c replicate 1
library_construction_protocol: RNA libraries for RNA-seq analysis were prepared using 500 ng of total RNA from three independent replicates using NEBNext Ultra II Directional RNA library prep kit for Il-lumina (NEB) and PolyA mRNA magnetic isolation module (NEB). Sequencing was performed using NextSeq 500/550 High-Output v2.5 (150 cycle) kit on NextSeq 550 platform (Illumina). Libraries were combined in a single equimolar pool of 12 based on Qubit and Bioanalyser results and run across a High Output v2.5 Flow Cell.