title: GSM8251786: E2C_m_NrKO_5; Mus musculus; RNA-Seq
library_construction_protocol: Extraction and library preparation were performed in one reaction (see below) The protocol employed is derived from FLASH-seq (V. Hahaut et al., “Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq.” Nat Biotechnol 40, 1447-1451; 2022), with two main modifications: 1/ Genotyping primers are added during the cDNA amplification step; 2/ A phosphate group is added to the 3' of the template switching primer to exclude the possibility it might prime DNA synthesis during the cDNA amplification step, which would result in tagging single transcripts with multiple UMI barcodes. Embryos were isolated and directly transferred by mouth pipetting to PCR tubes in 5ul of the following lysis solution: 0.1   μL Triton X-100 (10% v/v, Sigma-Aldrich, T8787); 1.2   μL dNTP mix (25 mM each, ThermoFisher Scientific, R1121); 0.46 μL 5'