title: GSM7510808: Biotinylation-DNA-seq_ctrl_rep1_R1; Homo sapiens; ChIP-Seq
library_construction_protocol: For antibody-based in situ biotinylation-seq, HeLa cells cultured in 10 cm dish were fixed with 4% PFA and permeabilized with 0.5% Triton X-100. After blocking, cells were incubated with primary antibodies, and then incubated with HRP-labeled secondary antibodies. After washing, HRP-based in situ biotinylation was performed by incubating cells with biotinylation buffer. The cells were lysed in 1% SDS RIPA buffer and were sonicated for DNA fragmentation. The biotinylated chromatin was pulled-down using SoftLinkTM Soft Release Avidin Resin (Promega), and the chromatin DNA was purified. The sequence libraries for in situ biotinylation DNA-seq were prepared using TruSeq ChIP Library Prep Kit (Illumina, San Diego, CA). The sequence libraries for in situ biotinylation RNA-seq were prepared using SMART-Seq Stranded Kit (Takara).