title: GSM7029593: DMSO_Control_BiologicalRepA [PGD-UnA]; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen RNeasy Mini kit was used for total RNA extraction. An on-column RNase-free DNase I treatment was included. RNA quality was assessed on the Agilent 2100 Bioanalyzer. Sequencing libraries were generated with 3 µg of RNA using the Illumina TruSeq Stranded mRNA Sample Preparation Kit. The libraries were assessed for size and purity using the Agilent 2100 Bioanalyzer, and quantitated by qPCR using a KAPA Biosystems Library Quantitation kit. Libraries were normalized, pooled, and diluted to a loading concentration of 1.8 pM, loaded on an Illumina High-Output Flow Cell, and sequenced on the Illumina NextSeq 500 instrument.