title: GSM8252023: ATAC_cDC1_WT_2; Mus musculus; ATAC-seq
library_construction_protocol: spleens were isolated and digested for 15 and 30 minutes respectively, in the presence of Liberase (50µg/ml, Sigma-Aldrich) and DNAse (250µg/ml, Sigma-Aldrich) in PBS supplemented with 5% FCS (Sigma-aldrich). Organs were then mashed through a 70µm filter and spleens were treated with ACK lysis buffer(BioLegend) for red blood cell elimination. Prepared cell suspensions were then pre-enriched using EasyStep pan-DC enrichment kit II (STEM cell) and later stained with FACS antibodies: cDC1 (Lin-CD11c+MHCII+CD64-XCR1+CD11b-), cDC2 (Lin-CD11c+MHCII+CD64-XCR1-CD11b+). Cells were FACS sorted and immediatelly processed for the transposase reaction using the Illumina Tagment DNA TDE1 Enzyme and Buffer kit. Tagmentation reaction was performed at 37ºC, for 30 minutes with 1000rpm incubation. DNA was the isolated using PCR purification kit (Qiagen). Nextera XT DNA Library Preparation Kit following user guide.