title: GSM6744646: HCT116 cells, SLAM_nolabeling_rep2; Homo sapiens; OTHER
library_construction_protocol: For Ribo-seq and RNA-seq, Direct-zol kit (Zymo Research) was used to extract RNA. For SLAM-seq we used TRIzol (Ambion), following the SLAM-seq kit (SLAMseq Kinetics Kit – Catabolic Kinetics Module, Lexogen). QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina, Lexogen for SLAM-seq, TruSeq Stranded Total RNA Human/Mouse/Rat kit (Illumina) for RNA-seq and a modified Ribo-seq protocol (see manuscript).