title: GSM8152918: ILC-enriched lung, Alt, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: The lungs were collected in free RPMI medium and then dissociated for single-cell suspension by the Miltenyi Lung Digest Kit and Dissociator. ILCs from Alt and Alt plus BPC treated mice were enriched from two single-cell suspended lungs by Mouse Pan-ILC Enrichment Kit. ILC enriched samples were preserved in PIPseqTM T100 3ʹ Single Cell Capture and Lysis Kit and processed by sequencing service. Library was performed according to manufacter's instructions (FB0003657 PIPseq T100 v3.0 Single Cell RNA Kit User Guide Rev 1.4, Fluent BioSciences).