title: GSM8289179: tp53 null, biol rep 1; Danio rerio; Bisulfite-Seq
library_construction_protocol: Thirty pooled 5 dpf larvae were flash frozen, then DNA extraction andmethylation libraries were generatedusing the Post-Bisulfite Adapter Ligation method. Starting material of 100ng of gDNA from bulk samples, one round DNA generation/random priming was used with four rounds of PCR following with a size selection step enriched for larger fragments of DNA. Uniquely indexed libraries were pooled and submitted for sequencing on the NovaSeq6000. Alignment and fractional methylation calls were generated using GemBS.