title: GSM7029103: PRAISE-WT-rRNA-untreated; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol (Invitrogen) followed by isopropanol precipitation, according to the manufacturer's instructions. For PolyA+ RNA isolation, size selection was first performed using MEGAclear Transcription Clean-Up Kit (Ambion) to deplete small RNA, and RNA was subsequently purified with two sequential rounds of polyA tail purification using oligo (dT)25 Dynabeads (Invitrogen). Small RNA fractions were collected from the flow-through of MEGAclear Transcription Clean-Up Kit. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols. tRNA libraries for RNA-seq were prepared using eCLIP library construction method.