title: GSM6801918: DLN/CD127-Tregs_2; Mus musculus; RNA-Seq
library_construction_protocol: Amplified cDNA for each sample was generated directly from sorted-Tregs using the SMART-Seq v4 Ultra Low Input RNA Kit for sequencing (Takara/Clontech) according to the manufacturer's instructions Barcoded cDNA libraries were generated with the Nextera XT DNA Library Preparation kit (Illumina) following manufacturer's protocols. cDNA sequenced with a 2 × 75 bp paired-end protocol with a v3 reagent kit on the Miseq (samples of DLN) and Nextseq 500 instruments (Illumina) (samples of spleen and graft) according to the manufacturer's instructions