title: GSM7224064: Male_SW_control_rna_rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol reagent and applied to a Phase Lock Gel-Heavy column. Eluted RNA was then rRNA depleted using the RiboCop  rRNA kit, according to the manufacturer's protocols Libraries were generated from 1mg of RNA using Lexogen's QuantSeq 39mRNASeq library kit. The concentrations of purified  libraries were quantified using Qubit and the average size was determined by a fragment analyzer with a  high-sensitivity NGS fragment analysis kit