title: PacBio reads from Calyptocephala attenuata whole body
design: High molecular weight genomic DNA was extracted from the whole body of four Calyptocephala attenuata adults using the Monarch HMW DNA extraction kit for tissue from NEB. The extracted DNA was sheared to between 15 kb and 20 kb using the Megaruptor 2 (Diagenode). A HiFi sequencing library was prepared using SMRTbell Express Template Prep Kit 2.0. Size selection of the final library was performed by the BluePippin System from SAGE Science. Fractions for sequencing were selected based on results from the Femto Pulse System. Desired size fractions were pooled and the final library was purified and concentrated using AMPure PB beads. Quantity of the final library was assessed using the Qubit dsDNA HS Assay Kit and the final size distribution was confirmed on the Femto Pulse