title: GSM6706026: WT_H3K4Me3; Mus musculus; ChIP-Seq
library_construction_protocol: Briefly, 10^5 T cells were collected and washed once with 500 μl wash buffer before they were bound to ConA beads for 10 min at 25 °C. After that, the cells were incubated with 1 μg rabbit anti-mouse H3K4me3 (Cell Signaling Technology, 9751, USA) antibody at 4 °C overnight. Goat anti-rabbit IgG was added and incubated for 1 h at 25 °C the next day. Then, the cells were washed three times with DIG wash buffer and incubated with 0.04 μM pA/G–Tnp for 1 h at 25 °C. Similarly, the cells were washed three times with DIG 300 buffer, resuspended in tagmentation buffer and incubated at 37 °C for 1 hour. Tagmentation was then stopped by the addition of proteinase K, buffer LB and DNA extract beads. After incubation at 55 °C for 10 min, the cells were plated on a magnet, and unbound liquid was removed. The beads were gently rinsed twice with 80% ethanol, and DNA was eluted with double-distilled water. Libraries were constructed using the 'TD903 Hyperactive Universal CUT&Tag Assay Kit for Illumina' and the 'TD202 TruePrep Index Kit V2 for Illumina' from Vazyme Biotech.