title: GSM7029090: PRAISE-WT-KAPA_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol (Invitrogen) followed by isopropanol precipitation, according to the manufacturer's instructions. For PolyA+ RNA isolation, size selection was first performed using MEGAclear Transcription Clean-Up Kit (Ambion) to deplete small RNA, and RNA was subsequently purified with two sequential rounds of polyA tail purification using oligo (dT)25 Dynabeads (Invitrogen).  RNA libraries for RNA-seq were prepared using KAPA Stranded mRNA-Seq Kit following manufacturer's protocols. 