title: GSM8114475: E7.5 rep1 Stat3null; Mus musculus; RNA-Seq
library_construction_protocol: chimeric embryos were collected at E7.5, E8.5, and E9.5 and pooled in 1.5 ml tube (~10 embryos for E7.5, ~ 4 embryos for E8.5, 1 embryo for E9.5). Embryos were dissociated into single-cell by TrypLE™ Express Enzyme and tdTomato+ and tdTomato- cells were sorted using a BD Influx sorter Sequencing libraries were prepared following 10x Genomics manufacturers protocol using 10x Single Cell 3' v3 chemistry.