title: GSM7329783: LT-HSC, WT-3-2nd; Mus musculus; RNA-Seq
library_construction_protocol: Different populations of hematopoietic stem/progenitor cells (LT-HSC, ST-HSC, CMP, GMP, MEP, CLP) from bone marrow cells of different genotypes of mice (WT, Sting-KO, Tet2-KO, Tet2/Sting-dKO) were sorted by  FACS with a number of 200 cells. The sorted cells were lysed with the lysis buffer obtained by SMART-seq construction Kit (Cat. N712, Vazyme) immediately and followed the library construction protocol from the Kit to generate cell full length mRNA library. RNA libraries of pre-malignant cells were constructed by Smart-seq2 protocol, Nat. Methods 10, 1096–1098 (2013). RNA libraries of mouse hematopoietic cells were constructed by kit (Vazyme, NR611)