title: GSM6745501: C57Bl6J mice, CD45+ cardiac cell, Sham control, 28 days, 2 [scRNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: CD45+ cells freshyl isolated from the heart were washed in PBS and submitted for scRNA-seq library preparation using the 10X Genomics Platform. Following QC, the single cell suspension was loaded onto Chromium Chip B (10X Genomics PN 2000060) and GEM generation, cDNA synthesis, cDNA amplification, and library preparation of 4,500-9,300 cells proceeded using the Chromium Single Cell 3' Reagent Kit v3 (10X Genomics PN 1000075) according to the manufacturer's protocol. cDNA amplification included 11 cycles and 44-87ng of the material was used to prepare sequencing libraries with 12 cycles of PCR. Indexed libraries were pooled equimolar and sequenced on a NovaSeq 6000 in a PE28/91 paired end run using the NovaSeq 6000 SP, S1, or S2 Reagent Kit (100 cycles) (Illumina).  An average of 201 million paired reads was generated per sample