title: DNA methylation of female tick: whole body
design: Treatment of DNA fragments with bisulfite was performed using EZ DNA Methylation-GoldTM Kit (Zymo Research), before polymerase chain reaction (PCR) amplification of the resulting single-stranded DNA fragments using KAPA HiFi HotStart Uracil + ReadyMix (2X) (Kapa Biosystems, Wilmington, MA, USA)