title: GSM7148379: Foxa2_HiChIP_GW; Mus musculus; OTHER
library_construction_protocol: About ~200 mg of each mouse liver tissue was pulverized using Covaris CryoPrep System with impact level setting 3. Pulverized tissues were washed with cold 1xPBS and pelleted by centrifugation at 1,000xg at 4˚C for 5 min.  HiChIP experiments were performed with vehicle- and ligand-treated mouse liver tissues using the Arima-HiC+ Kit (Arima Genomics, Cat. A101020) according to manufacturer's  protocol.   Foxa2‐specific rabbit antiserum (Seven Hills Bioreagents, WRAB‐1,200) was used for immunoprecipitation. HiChIP libraries were prepared using Accel-NGS 2S Plus DNA Library Kit for Illumina Platforms (Swift Biosciences, Cat# 21024) and Accel-NGS 2S Indexing Kit (Set A) for illumine Platforms (Swift Biosciences, Cat# 26148)