title: scRNA-seq of zebrafish intestine: MTZ_48 hpt
design: Single Cell 3 Gene Expression libraries comprise standard Illumina paired-end constructs which begin with P5 and end with P7. 16 bp 10x Barcodes are encoded at the start of TruSeq Read 1, while 8 bp sample index sequences are incorporated as the sample index read. TruSeq Read 1 and Read 2 are standard Illumina sequencing primer sites used in paired-end sequencing. TruSeq Read 1 is used to sequence 16 bp 10x Barcodes and 36 bp UMI. Sequencing these libraries produce a standard Illumina BCL data output folder.