title: GSM7029635: Multi-genotype zebrafish skin snRNAseq; Danio rerio; RNA-Seq
library_construction_protocol: Fish were staged and selected for dissection, euthanized with MS-222 and processed immediately. Following removal of the head and fins, skins were removed with forceps and immediately flash frozen in liquid nitrogen then stored at -80°C prior to isolation of nuclei. 3' end capture of polyAdenylated transcripts. In situ reverse transcription with barcoded RT primers followed by second strand synthesis, tagmentation and index PCR gives each nucleus and all the RNA molecules contained within it a unique combination of barcode sequences. These barcodes are then demultiplexed to create single cells in silico.