title: GSM7031360: ATAC_P14_Cl13_D7_TerminallyExhaused_Control_3; Mus musculus; ATAC-seq
library_construction_protocol: P14 cells isolated from spleen were sorted for scRNA-Seq, mutiome or ATAC-Seq anallysis. Sorted CD8 T cells were used for ChIP-Seq experiment. We used Chromium Next GEM Single Cell 5' Kit (10x Genomics) to generate scRNA-Seq libraries according to the manufacturer's protocol. ATAC-Seq libraries were generated using a FASTATAC protocol (Corces, M. R. et al. Nat. Genet. 48, 1193–1203 (2016)). We used Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits (10x Genomics) to generate scRNA-Seq-multiome and scATAC-Seq-multiome libraries according to the manufacturer's protocol. ChIP-Seq was performed according to the published chipmentation protocol (Schmidl, C., Rendeiro, A. F., Sheffield, N. C., & Bock, C. (2015).http://doi.org/10.1038/nmeth.3542).