title: RNA-Seq of mus musculus
design: According to the instruction manual of the TRlzol Reagent (Life technologies, California, USA), the total brain was extracted, measured RNA concentration, purified by NanoDrop 2000 (Thermo Fisher Scientific, Wilmington, DE), and assessed RNA integrity by RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA).