title: GSM7598101: (2) rhIL-7-hyFc [TCR]; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized at indicated time points and tumor tissues were harvested. Tumor tissues were mechanically dissected and then digested with collagenase D (400 units/mL; Roche) and DNase I (200 ug/mL; Roche) in washing buffer (2% NCS plus Antibiotic-Antimycotic in RPMI-1640) for 30 min in a shaking incubator at 37 °C. The enzyme-digested tumor meshed over a 70-um cell strainer. Red blood cells (RBC) were lysed with RBC lysing buffer (Sigma-Aldrich). For the enrichment of CD8 T cells, cells were isolated by positive selection methods with IMag according to the manufacturer's protocol (condition 1) or sorted by FACS (GhostDyeCD45+TCRb+CD8a+) (condition 2) after single-cell preparation. The purified CD8 T cells were analyzed by scRNA-seq paired with scTCR-seq. Chromium Next GEM Single Cell 5p RNA library v2 for gene expression and Chromium Next GEM Single Cell VDJ library v2 for TCR seq