title: GSM8196754: 5448_stat_input; Streptococcus pyogenes; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 20 min. After quenching and cell lysis, sonication was performed with a Branson Sonifier 450. Cas9 IP and input control libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina®. 1 ng DNA was used as input and 9 cycles were used for PCR amplification.