title: GSM7734766: WT sphere (4 hpf) RNA-seq rep2; Danio rerio; RNA-Seq
library_construction_protocol: 50 whole embryos were collected per sample and immediately lysed in 800uL LiDS/LET buffer(5% Lithium dodecyl sulfate in 20 mM Tris-HCl pH=7.4, 100 mM LiCl, 2 mM EDTA, 5 mM DTT, pH 7.4). 250 ng of the same total RNA used for mim-tRNAseq library preparation were used for mRNA-Seq library construction with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). Libraries were sequenced on a NextSeq 500 platform.