title: RNAseq of homo sapien Hep3B cells treated with PBS
design: RNA isolation: RNeasy mini kit (Cat. #74106, Qiagen). QC & Quant: TapeStation (Agilent Technologies) and Qubit 2.0 Fluorometer (ThermoFisher Scientific). Library prep: NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). Library validation and quantification: TapeStation (Agilent Technologies), Qubit 2.0 Fluorometer (ThermoFisher Scientific) and quantitative PCR (KAPA Biosystems). Libraries were multiplexed and sequenced on Illumina NextSeq 550 using 2x150bp paired end configuration.