title: GSM7734932: Fetal heart, 8.30 weeks of gestation, sample 3 (RNAseq); Homo sapiens; RNA-Seq
library_construction_protocol: whole hearts were stored at -80 °C in RNAlater Stabilization Solution (Thermo Fisher Scientific). After thawing, ventricles were isolated from the atria and major vessels. To eliminate blood inside the chambers, the ventricles were cut open, rinsed with PBS for 10 seconds and kept in TRIzol. Ventricles were then homogenized at 30 kHz for 1 min using TissueLyser II (Qiagen) and centrifugated to pellet the insoluble tissue. DNA-free RNA was extracted using a Direct-zol RNA MiniPrep kit (Zymo Research). RNA-seq libraries were prepared using a NEBNext Ultra RNA Library Prep Kit for Illumina with the NEBNext Poly(A) mRNA Magnetic Isolation Module and 1 ug of starting material.