title: Whole Exome Sequencing of Mus Musculus: luciferized CT2A cells grown in vivo
design: DNA was extracted with the PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, Waltham, MA) and the sequencing libraries were prepared using the SureSelectXT Mouse All Exon Kit(Agilent, Santa Clara, CA) at GENEWIZ. Fragmented DNAs were cleaned up, end-repaired, and adenylated at the 3ends. Adapters were ligated to the DNA fragments, and the ligated products were then enriched with limited cycle PCR. 200 ng of adapter-ligated DNA fragments were hybridized with biotinylated RNA baits at 65 C for 24 hours. Hybrid DNAs were captured by streptavidin-coated magnetic beads, washed, and then amplified with Illumina indexing primers.