title: Illumina HiSeq 1500 sequencing; Whole Transcriptome Analysis of Substantia Nigra in Mice with MPTP-Induced Parkinsonism Bearing Defective  Glucocerebrosidase Activity
design: Whole Transcriptome Analysis of Substantia Nigra in Mice with MPTP-Induced Parkinsonism Bearing Defective  Glucocerebrosidase Activity
library_construction_protocol: Sixteen mice C57BL/6 8–12 weeks old weighing 22–26 g were generated and separated into four groups with four animals in each, and treated with the following  solutions: 0.9% sodium chloride solution (vehicle mice), conduritol -epoxide (CBE) with MPTP (MPTP-CBE), CBE, MPTP. The brain samples were removed from the skull and cut along the midsagittal plane. The substantia nigra was dissected under a dissecting microscope with an ocular micrometer (Nikon SMZ660, Nikon, Melville, NY, USA).  Total RNA was extracted from brain tissue with Trisol reagent and PureLink RNA micro Kit (PureLink RNA micro Kit, Invitrogen, CA, USA ), according to the manufactur-er's instruction. PolyA RNA was purified with Dynabeads® mRNA Purification Kit (Dynabeads® mRNA Purification Kit, Ambion, TX, USA ).  The Illumina library was made from polyA NEBNext® Ultra™ II RNA Library Prep (NEB-Next® Ultra™ II RNA Library Prep, NEB, MA, USA ), according to the manual.