title: GSM7329704: peripheral blood cells, inactive LN 13; Homo sapiens; RNA-Seq
library_construction_protocol: Purified RNA was used to prepare a sequencing library using the TruSeq  Purified RNA was used to prepare a sequencing library using the TruSeq stranded mRNA library prep kit. mRNA was purified from total RNA (1 μg) on oligo(dT)-coupled magnetic beads and fragmented into small pieces under elevated temperature. First-strand cDNA was synthesized using reverse transcriptase and random primers. After generating double-strand cDNA and adenylation of the 3' ends of the DNA fragments, the adaptors were ligated and then the samples were purified using the AMPure XP system.