title: GSM7225470: IOMM-Lee, 78 nM digitoxin + 2 nM torin 2, 24h, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using the Rneasy kit (Qiagen). RNA yields of ranging from 270-870 ng were used for the construction of sequencing libraries. RNA libraries for RNA-Seq were prepared using the Illumina Stranded RNA Library Preparation kit from 100ng of high-quality RNA samples (RIN > 7) according to the manufacturer's instructions. This procedure includes mRNA purification and fragmentation,   cDNA synthesis, 3' end adenylation, Illumina adapter ligation, library PCR   amplification and validation. Illumina sequencer was used to sequence the   libraries with the production of single-end, 50 bp reads at the depth of   20-25 M reads per sample.