title: GSM6804952: MA9 BM, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: BM cells in MA9 transplanted mice were collected for analysis. Cells were resuspended as single cells in the presence of 0.4% BSA and loaded onto a 10x Genomics Chromium instrument to generate an emulsion of single-cell gel beads (GEMs). Approximately 5,000-10,000 cells were loaded per channel. scRNA-seq libraries were prepared using the Chromium™ Single Cell 3′ Library & Gel Bead Kit v2 (PN-120237), Single Cell 3′ Chip Kit v2 (PN-120236) and i7 Multiplex Kit (PN-120262) (10x Genomics, Pleasanton, CA, USA), following the Single Cell 3′ Reagent Kits v2 User Guide (Manual Part # CG00052 Rev A). Libraries were sequencedon an Illumina NovaSeq system as 2 × 150 paired-end reads, one full lane per sample, for >90% sequencing saturation.