title: GSM6850277: OLG, Input; Mus musculus; ChIP-Seq
library_construction_protocol: The cells were washed with PBS (to remove cell debris), fixed in 1% PFA for 10 min, incubated with glycine, and then were flash frozen.  After quenching and cell lysis, chromatin was sonicated using aBioruptor, Diagenode with high power with 40 cycles of 30 sec “on”, 45 sec “off”. chromatin was incubated over night with 4.5ug antibody, washes was done acording to: Hojo H, Ohba S, He X, Lai LP, McMahon AP. Sp7/Osterix Is Restricted to Bone-Forming Vertebrates where It Acts as a Dlx Co-factor in Osteoblast Specification. Dev Cell. 2016 May 9;37(3):238-53. doi: 10.1016/j.devcel.2016.04.002. Epub 2016 Apr 28. PMID: 27134141; PMCID: PMC4964983. ChIP-seq libraries were prepared  essentially as described in PMID: 23429716.  Libraries were amplified using 14 cycles on the thermocycler.  Libraries were validated using tape station and Qubit.