title: GSM7777870: senescent, METTL3, HiChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: IMR90 cells were fixed in freshly made 1% formaldehyde at room temperature for 10 min followed by quenching with 125 mM glycine. Samples was lysed in ice-cold Hi-C lysis (10 mM Tris, pH 8, 10 mM NaCl, 0.2% IGEPAL CA-630 with protease inhibitors) buffer and digested with MboI for 2 h at 37°C. IP samples were reversed-crossed, pulled down with streptavidin C1 beads and went through tagmentation by transposaseTn5. Samples were amplified with certain cycle numbers according to qPCR with 5-cycle pre-amplified library.