title: GSM7735239: Blood, patient 1; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA from the peripheral blood samples was isolated using a PAXgene Blood RNA Kit (Qiagen) according to the manufacturer's protocol.RNA concentration was quantitated using the NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE). 1) 3'-adaptor ligation;2) 5'-adaptor ligation;3) cDNA synthesis;4) PCR amplification;5) size selection of 135   155 bp PCR amplified fragments (corresponding to 15   35 nt small RNAs).