title: GSM7508851: NL3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested usingTRizol reagent and Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. Samples treated with Dnase to avoid genomic DNA contamination. Preparation of dual-indexed, strand-specific RNASeq library from submitted total RNA was performed using the NEBNext polyA selection and Ultra II Directional RNA library preparation kits (New England Biolabs, UK).