title: GSM6805259: Y184 bcy1∆ YPX Rep D; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Hot phenol lysis followed by DNA digestion with Turbo-Dnase. rRNA was depleted with EPiCenter Ribo-Zero Magnetic Gold Kit (Yeast) RevA kit (Illumina Inc, San Diego, CA), and the remaining RNA was purified using Agencourt RNACleanXP (Beckman Coulter, Indianapolis, IN) by following the manufacturers' protocols. RNA-seq libraries were created with the Illumnia TruSeq stranded total RNA kit (Illumina) following the preparation guide (revision C), AMPure XP beads were used for PCR purification (Beckman Coulter, Indianapolis, IN), and cDNA generated with SuperScript II reverse transcriptase (Invitrogen, Carlsbad, CA) as described in the Illumina kit.