title: GSM6604102: siCTRL_input1; Homo sapiens; RIP-Seq
library_construction_protocol: RIP-seq was used to detect the mRNA binding sites of FBL in C4-2 cells. C4-2 cells were lysed in RIP lysis buffer and incubated with FBL antibody (anti-FBL (Abcam, Cat#ab5821))-magnetic beads mixture at 4 °C overnight to enrich RNA binding protein. The RNA that bound to FBL protein were collected by Proteinase K digestion, followed by purification and precipitation. The RIP experiment in C4-2 cells was performed using the EZ-Magna RIP kit (Millipore) with the procedure provided by the manufacturer. For high-throughput sequencing, input and RIP-ed RNA samples were shipped to BGI for library preparation and sequencing using the DNBseq platform.