title: GSM7511024: Control; Mus musculus; RNA-Seq
library_construction_protocol: Briefly, following euthanasia, the lower-jaw was removed from a P20 control and Memo1;K14CRE mouse (N = 1) and each hemimandible cleaned of soft tissue in ice-cold DEPC-PBS. The cervical loop was exposed by gently shaving away the bone with a fresh scalpel and collecting the tissue (including some enamel), using the second molar as a distal delimiter. The region harvested included both epithelial and mesenchymal components of the cervical loop. Following tissue harvest, samples were incubated in a trypsin/dispase 'cocktail' for ~35 minutes at 37°C and dissociated into a suspension of single cells. Any hard, non-dissociated tissue (i.e., mineralized enamel) was allowed to settle briefly, and the supernatant transferred. Cells were washed 2x with PBS+BSA, resuspended in PBS+BSA, and subsequently passed through a Scienceware Flowmi cell strainer (40 µM). After cell density quantification, ~10,000 cells from each suspension were used to generate scRNAseq libraries, using the standard 10x genomics platform. Libraries were prepared using the 10x Genomics 3' end single cell RNA-seq platform.