title: GSM8196603: Mono-derived DC, Mf live, rep4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from DCs using the Total RNA and Protein Isolation Kit (Thermo Fisher Scientific). The mRNA was purified from total RNA using the RNeasy Pure mRNA Bead Kit (Qiagen) according to manufacturer instructions. Samples with RIN values above 8.0 were prepared for library preparation. Indexed RNA sequencing (RNA-seq) libraries were constructed from 1 µg total RNA using a TruSeq Stranded mRNA Library Prep (Illumina). The mRNA-Seq libraries were sequenced in paired-end mode (2x151 bp) on Illumina NextSeq sequencer.