title: GSM6917951: 702549-monocytes; Homo sapiens; RNA-Seq
library_construction_protocol: Fluorescence-activated cell sorting was performed to obtain isolated amniotic fluid neutrophils and monocytes/macrophages. Isolated cells were kept in RLT buffer (Qiagen) prior to RNA isolation. RNA isolation was performed using the Qiagen Micro Rneasy kit, and RNA purity, concentrations, and integrity were assessed. Low-input RNAseq libraries were constructed by Beijing Genomics Institute (BGI; Wuhan, China) using the Smart-Seq2 kit (Illumina).