title: GSM7329612: A375_DSE_u_63-EGFP sgTRAC B; Homo sapiens; ssRNA-seq
library_construction_protocol: One million cells were then harvested by trypsinization and pelleted by centrifugation at 500 x g for three minutes. Pellets were washed once with PBS (Sigma-Aldrich, D8537), flash frozen and stored at -80C until use. The pellet was resuspended in 350ul RLT buffer (Qiagen, 74106)  supplemented with 1% beta-mercaptoethanol. The lysate was homogenised using the Qiashredder column (Qiagen, 79654) and RNA extracted utilising the RNeasy Plus Mini Kit (Qiagen, 74136) according to manufacturer's instructions. RNA concentration was measured with  the Qubit RNA HS assay kit (Life Technologies, Q32852) according to  manufacturer's instructions and methanol flash frozen. RNA-seq libraries were generated and sequence through BGI service for strand specific RNA-seq with PolyA selection (DNBseq Eukaryotic Transcriptome De novo Sequencing).