title: GSM7882954: RNA_BAN-2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: The cells were filtered using 0.45µm MCE membrane and lysed in lysis buffer using the beads shocker. For ribosome profiling, RNase I treatment, ribosome pelleting, size selection, preadenylated linker ligation, rRNA depeletion by riboPOOL for Saccharomyces cerevisiae (siTOOLs), reverse transcription, cDNA circularization, and PCR amplification. For RNA-Seq, SEQuoia Express Stranded RNA Library Prep Kit (Bio-Rad).