title: RNA-Seq of salmon: hindgut juvenile salmon
design: total RNA was extracted from 20 mg of each sample using the E.Z.N.A. Total RNA Kit II (Omega Bio-tek). Each RNA sample was treated using DNase I (Promega) and then cleaned with RNA Elite beads (MagBio). Libraries were constructed using the NEB Next Ultra II RNA Library Prep Kit and sequenced at the Institute for Integrative Genome Biology at the University of California, Riverside (USA).