title: GSM8027552: NFKB-, biol rep 3; Danio rerio; RNA-Seq
library_construction_protocol: RNA was then isolated and purified with RNeasy® Micro Kit (Qiagen). DNase I treatment was performed as specified by the manufacturer. Total RNA was assessed for quality and quantity using an Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit. Triplicate RNA samples of each condition were processed following manufacturer's instructions. Samples were then used to generate RNA sequencing libraries using NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB #E6420S/L) for Illumina and NEB's Unique Dual Index primers for Illumina (E6440).