title: GSM7065162: PRFKO1; Mus musculus; OTHER
library_construction_protocol: fluorescence-activated cell sorting directly into lysis/stabilization media Messenger RNA purification and fragmentation, complementary DNA synthesis and target amplification were performed with TakaraBio SMARTer Mouse TCRa/b Profiling Kit. Pooled complementary DNA libraries were sequenced on the Illumina NextSeq 500.