title: GSM7031539: Human splenocytes, mRNA-derived cDNA; Homo sapiens; OTHER
library_construction_protocol: Stained samples were adjusted to a concentration of 1200 cells/μL and loaded onto a Chromium controller (10X Genomics) as per manufacturer protocol for GEM generation and barcoding. Samples were prepared for sequencing using the 10X Genomics Single Cell 5' v2 platform in accordance with manufacturer's instructions for capture of 12,000 cells per sample. Reverse transcription, cDNA amplification and sequencing libraries were generated using 10X Genomics Single Cell 5' v2 reagents. Across samples, cells were sequenced to a target depth of 40,000 reads per cell. ADT and RNA libraries were sequenced in separate lanes on a shared flow cell.