title: GSM7601213: EPIA09RNA_Patient_OSA; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Qiagen RNeasy purification procedure and mRNA was purified using oligo(dT)-attached magnetic beads. First, the mRNA molecules were fragmented and converted into cDNA through reverse transcription using random hexamer primers. Following this, the cDNA fragments were processed by end-repair, adenylation, and adapter ligation, and then amplified through PCR to enrich the cDNA fragments. After purification, the library's quality was assessed using the Agilent Technologies 2100 bioanalyzer. Finally, the double-stranded PCR products were denatured, circularized using a splint oligo sequence, and amplified with phi29 DNA polymerase to generate DNA nanoballs (DNBs), each containing more than 300 copies of a single molecule, forming the final library.