title: Illumina HiSeq 4000 sequencing; mRNA-seq of HCC1806 cells, treated with siControl or siRNF40 for 48 hours
design: mRNA-seq of HCC1806 cells, treated with siControl or siRNF40 for 48 hours
library_construction_protocol: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using NEXTflex™ Rapid Illumina Directional RNA-Seq Library Prep Kit (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.