title: GSM7777757: wild type_BiolRep2; Schizosaccharomyces pombe; OTHER
library_construction_protocol: RNA was extracted with QIAzol and RNeasy Mini kit (Qiagen) according to the manufacturer's recommendations, including a DNase treatment. RNA quality was assessed in an Agilent 2100 Bioanalyzer and strand-specific cDNA libraries were made NEXTflex Rapid Directional qRNA-Seq Kit (PerkinElmer, formerly BiooScientific). Libraries were quantified in a Qubit, pooled and 4 nM of the pooled library was sequenced