title: GSM8251636: BrUseq, CFAP20KO, rep3; Homo sapiens; OTHER
library_construction_protocol: After incubation cells were lysed in Trizol (Thermo Fisher, 15596018) and BrU-containing RNA was isolated as previously described (Andrade-Lima et al., 2015, Nucleic Acids Res) cDNA libraries were made from the BrU-labeled RNA using the Illumina TruSeq library kit and paired-end 151 bp sequenced using the Illumina NovaSeq platform at the University of Michigan Advanced Genomics Core. Paired-end sequencing data was used for downstream analyses. nascent RNA-seq