title: GSM6705855: Pi_deficient_phr1phl3_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNAs were isolated from roots of 8-day-old seedlings of the WT and various mutants grown under Pi sufficiency and deficiency using the RNeasy plant mini kit (Qiagen, catalog: 74904). mRNAs were purified from total RNAs using Oligo d(T) beads and were reversely transcribed into cDNAs. The cDNAs were fragmented, and sequencing libraries were prepared by end repairing, “A” tail addition, and adaptor ligation.