title: RNA-Seq of Z. viviparus female haltered at 12 C water temperature: liver tissue
design: RNA-Seq libraries were generated from liver tissue of Z. viviparus specimens haltered at 12 C water temperature. Total RNA was extracted from 5-10 mg liver tissue using the QIAGEN RNeasy Kit (QIAGEN). mRNA enrichment was performed with 100 ng total RNA as input, followed by poly-A selection and cDNA library construction using the VAHTS Universal V8 kit (Vazyme).