title: GSM6745360: 953_Miso_Female_HSC; Mus musculus; RNA-Seq
library_construction_protocol: Qiagen RNeasy Plus Micro kit Total RNA samples were first evaluated for their quantity and quality using Agilent Bioanalyzer 2100. All samples had good quality with RIN (RNA Integrity Number) greater than 7. One nanograms of total RNA was used for library preparation with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech, Mountain View, CA) and the Nextera XT DNA Lib Kit (Illumina, San Diego, CA) following the manufacturer's protocol. Each resulting uniquely dual-indexed library was quantified and quality accessed by Qubit and Agilent TapeStation, and multiple libraries were pooled in equal molarity. The pooled libraries were sequenced with 2×100bp paired-end configuration on an Illumina NovaSeq 6000 sequencer using the v1.5 reagent kit.