title: GSM7818602: NSG-mdx iPAX7 Replicate 2; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from iPAX7 in vitro-differentiated myotubes and homogenized tibialis anterior muscles using TRIzol (Invitrogen) and the PureLink RNA mini kit (Invitrogen, #12183025) with on-column DNaseI treatment following the manufacturer's recommendations. 500 ng of total RNA was used to for generate unique dual-indexed (UDI) TruSeq stranded mRNA libraries.