title: GSM6604382: RNA-seq_KO3; Homo sapiens; RNA-Seq
library_construction_protocol: For the bulk RNA sequencing, total RNA was extracted from undifferentiated wild-type and S6K1-depleted embryonic stem cells using miRNeasy Kit (Qiagen). A library construction for bulk RNA sequencing was conducted with TruSeq Stranded mRNA Sample Preparation Kit (Illumina) according to the manufacturer's instruction.