title: GSM8249554: S4_MiEG; Mus musculus; RNA-Seq
library_construction_protocol: After GFP-sorting, cell pellets were resuspended in RNA lysis buffer (RNeasy Kit, Qiagen) and stored at -80°C before being processed using RNAeasy Kit (Qiagen) according to manufacturer's protocol. For RNA-seq, 1 ug of total RNA was used. Preparation of barcoded mRNA-seq library and sequencing using NextSeq500 platform (Illumina) with paired-end reading at 75 bps read-length were performed at the EMBL Genomics Core Facility (Heidelberg, Germany)