title: GSM7437224: C1-D7; Mus musculus; OTHER
library_construction_protocol: Mice were deeply anesthetized with pentobarbital and perfused transcardially with cold (4–8C) isolation buffer.. The forebrain hemisphere was isolated by dissection of the olfactory lobes and the midbrain structure (bregma from ~ +3 to -4 mm). Dissected forebrains were embedded in optimal cutting temperature (OCT) medium, rapidly placed on dry ice to freeze, and then transferred to -80C for a maximum of six weeks of storage. The forebrains were sectioned coronally (10 um thickness) using Leica CM1950 cryostat (Leica Microsystems). Sections collected for 10X Visium Spatial Gene Expression processing were stored at -80C. Sequencing libraries were prepared strictly according to the manufacturer's manual “Visium Spatial Gene Expression Reagent Kits User Guide” (CG000239).