title: Single cell RNA Seq to study the effects of kinase-inactivated Cdk6 or a Cdk6 knock-out in the mouse LSK cell compartment
design: Single cell RNA Seq to study the effects of kinase-inactivated Cdk6 or a Cdk6 knock-out in the mouse LSK cell compartment
library_construction_protocol: 30000 lineage-negative, Sca1-positive, cKit-positive (LSK) cells were isolated from mouse bone marrow by means of flow cytometry cell sorting. Nucleic acid extraction was performed as part of the library construction. Libraries were generated using the Chromium NextGem Single Cell 5' v2 Kit solution (10X Genomics), according to the manufacturer's instructions