title: GSM7818858: LCM_seq_p112wtSC [P6362_1121]; Mus musculus; RNA-Seq
library_construction_protocol: Cells isolated by Laser Capture microdissection were placed in lysis buffer containing 0.2%Triton X-100 with 2U/ul recombinant RNAse inhibitor (Clontech) Bulk libraries were prepared using the Smart-Seq2 protocol.  One ng of cDNA was used as input for the tagmentation which was carried out with in-house Tn5 (Piccelli S. 2014, Nat. Protoc. 9, 171-181). For ligation of sequencing indices we used Nextera XT Sequencing Index Kit (Illumina) and 10 cycles of enrichment PCR.