title: GSM8250641: NRP1-MDA.MB.231-shNRP1.5-RNA.3; Homo sapiens; ssRNA-seq
library_construction_protocol: Cells were lysed with TriZol reagent (Invitrogen, Thermofisher Scientific). Total RNA isolation was performed using the Direct-zol RNA Miniprep Plus kit (Zymo Research) as per manufacturer's instructions. The 2100 Bioanalyzer (Agilent Technologies) was used to determine the RNA integrity number (RIN), with the RNA 6000 Nano Kit (Agilent Technologies) according to manufacturer's instructions used for sample preparation. Samples with a RIN >8 were submitted for sequencing. RNA libraries for RNA-seq were prepared using the Illumina TruSeq Stranded mRNA Sample Prep Kit (strand-specific, polyA enrichment), no ERCC controls. Input for each sample was 500 ng.