title: Examination of 16S rRNA gene amplicons extracted from nasal swabs of beef calves in Texas: A Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease.
design: The aim of this study is to evaluate the nasal microbiome of apparently healthy (n=40) and BRD-affected (n=46) pen-mate beef calves, exploring its potential as an additional diagnostic tool for identifying BRD. Samples were obtained from a farm in Texas. The analysis of the nasal microbiome included amplification and sequencing of the V4 region of the 16S rRNA gene using an Illumina MiSeq Sequencer.