title: ChIP-seq using anti_HA antibody of HA-tagged P. falciparum NF54 at trophozoite stage
design: iRBCs were collected at P. falciparum trophozoite stage and chromatin crosslinked using 1% formaldehyde. Chromatin was sheared using the Covaris S220 ultrasonicator and immunoprecipitated with anti-HA antibody. DNA was recovered by phenol/chloroform extraction and ethanol precipitation and purified using AMPure XP beads. Libraries were then prepared with the KAPA LTP Library Preparation Kit.