title: GSM7225648: HDF1_shAR_LamAC ChIP-Seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were lysed and chromatin was prepared using the Diagenode Chromatin EasyShear Kit - High SDS (Cat# C01020012). Chromatin was sheared using the Bioruptor with 4 rounds of 10 cycles of 30 seconds “ON” / 30 seconds “OFF” at HIGH power setting (position H). The chromatin fractions were diluted and ChIP reactions were carried out using anti-Lamin A/C antibodies using the Diagenode kit as recommended. Library preparation were carried out using NEBNext ChIP-seq Library Prep Reagent Set for Illumina (E6200) as per the manufacture's protocol