title: GSM8421891: HS_2; Homo sapiens; RNA-Seq
library_construction_protocol: Human scalp samples were digested in 4 mg/ml Dispase (Stem Cell) overnight. Individual HFs were then pulled out one by one using forceps. To obtain single-cell suspension for scRNAseq, the isolated HFs were digested in 0.05% Trypsin-EDTA (Gibco) for 30 mins at room temperature, washed in DMEM + 10 % FBS, and filtered through a 100 μm cell strainer. HF cells were sorted (BD InfluxTM Cell Sorter) using a nuclear labeling DRAQ7TM Far-Red Fluorescent Live-Cell Impermeant DNA Dye (Abcam, ref:  ab109202). Cells were collected in 20% FBS-RPMI solution. Around 25,000 cells were loaded into one channel of the Chromium system using the V3.1 single-cell reagent kit (10X Genomics) to generate single-cell GEMs. Following capture and lysis, cDNAs were synthesized, and then amplified by PCR for 12 cycles as per the manufacturer's protocol (10X Genomics). The amplified cDNAs were used to generate Illumina sequencing libraries that were each sequenced on one flow cell Nextseq500 Illumina.