title: GSM8027536: LCL_Ctrl_1_Basal; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from LCLs stimulated or not with IL-27 for 6 h hours using the RNeasy Kit (QIAGEN) including DNAse treatment. RNA quality was assessed by capillary electrophoresis by Fragment Analyzer using High Sensitivity RNA reagents (Agilent Technologies) and RNA concentration measured by spectrophotometry withXpose (Trinean) and Fragment Analyzer. RNAseq libraries were generated from 600 ng of RNA using Universal PlusTM mRNA-Seq with NuQuant® (NuGEN, San Juan, Puerto Rico)