title: GSM7883907: pbmc; Sus scrofa domesticus; RNA-Seq
library_construction_protocol: Peripheral blood samples (2 mL) were collected into EDTA anticoagulant tube and gently inverted 2-3 times to ensure thorough mixing. Subsequently, the mixed blood samples were transferred to 15 mL centrifuge tube and treated with 6 mL of red blood cell lysis solution for 15 min, followed by centrifugation at 1600 rpm for 10 min at 4℃ to collect cell pellets. The cell pellets were then re-suspended in cold phosphate-buffered saline (PBS), passed through a 100 μm cell strainer, and incubated with 3 μL CD3ε antibody (clone BB23-8E6-8C8, BD Pharmingen) for 30 min at 4℃ in the dark, followed by FACS to obtain living CD3+ PBMCs. The cell viability assay using trypan blue staining. After cell counting, the samples from the three healthy pigs were mixed in equal volumes to obtain 9×105 cells. The mixed cells were used for constructing 10× genomics sequencing library and subsequent sequencing by Genergy Biotechnology (Shanghai, China) RNA libraries were prepared with Chromium Single cell 3'Reagent v2 Kits according to the manufacturer's protocol.