title: GSM8152440: Placenta; Sus scrofa; RNA-Seq
library_construction_protocol: Single nuclei were isolated from the samples using Pure Prep Nuclei Isolation kit (Product No. NUC-201, Sigma, St. Louis, MO, USA) as per manufacturer's instructions. The nuclei were used to prepare sequencing libraries using 10X Genomics Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 at the University of Missouri Genomics Technology Core according to manufacturer's specifications. 10X Genomics Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1. Each library was sequenced to a depth of 20,000 paired-end (single-indexing) reads per nucleus.