title: 12S DNA metabarcoding of eDNA: freshwater sample
design: DNA extracted from residue collected on glass microfiber filter papers using a Qiagen DNeasy kit. Amplicon library prepared using two-step PCR. First PCR step used MiFish 12S primers and second step added index sequences for each sample using Illumina TruSeq primers. Libraries were pooled and purified using Ampure XP magnetic beads and quantified via QuBit broad range kit. Pooled libraries were sequenced by Illumina MiSeq using the V2 kit (150bp x 2).