title: GSM7065327: heart_P635L_hom_mouse_NRCH_gRNA5_rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was perfomed on 200 ul of homogenized tissue with 1 ml TRIzol (ThermoFisher), and then processed using the Direct-zol RNA Miniprep Kit with on-column DnaseI digestion. 200-500 ng RNA was used as input for reverse transcription with SuperScript IV (ThermoFisher). RNA sequencing libraries were prepared using the NEBNext Ultra II Directinal RNA Library Prep Kit following manufactrurer's instruction.