title: GSM7819316: ZHX2_Input; Rattus norvegicus; ChIP-Seq
library_construction_protocol: The tissue was collected and frozen in liquid nitrogen. The cells were treated with cell lysis buffer, and the nucleus was collected by centrifuging at 2000g for 5 min. The nucleus was treated with nucleus lysis buffer and sonicated to fragment chromatin DNA. The 10% lysis sonicated chromatin was stored as "input", and 90% was used in immunoprecipitation reactions with anti-ZHX2 antibody (Proteintech, 20136-1-AP) named "IP". The DNA of input and IP was extracted by the phenol-chloroform method. The high-throughput DNA sequencing libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina V3 (Catalog NO. ND607, Vazyme). The library products corresponding to 200-500 bps were enriched.