title: GSM8326752: 1571, hSS1; Mus musculus; RNA-Seq
library_construction_protocol: Fresh frozen tumor tissue was disrupted using Tissue-Tearor (BioSpec) in 1ml of TRIzol™ reagent (ThermoFisher).  RNA was extracted with Zymo Direct-zolTM RNA Miniprep kit. Total RNA samples (5-500 ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Human, Mouse, Rat) (E7400) to substantially diminish rRNA from the samples.  Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L).  Purified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583).  The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Individual libraries were normalized and pooled in preparation for Illumina sequence analysis.