title: GSM7030373: Control 3; Lactuca sativa var. capitata; RNA-Seq
library_construction_protocol: Lettuce roots from each treatment (PS, PS+CH) were sampled at 72 hpt. Five biological replicates per treatment/timepoint combination were randomly selected. The soil on the roots was washed off and roots were pooled, flash frozen in liquid nitrogen and stored at -80°C before further processing. Total RNA was extracted using the CTAB method as described in detail by Luypaert et al. (2017). Contaminating DNA was removed using DNA-free DNA removal kit (AM1906, Invitrogen; Thermo Fisher Scientific, Inc., Waltham, USA, Massachusetts) according to the manufacturer's protocol. To study the overall transcriptional reprogramming in lettuce upon chitin treatment, in total five RNA samples per treatment sampled at 72 hpt were shipped to BGI Tech Solutions Co. Ltd. (Hong Kong, China) for cDNA synthesis, library preparation, and sequencing.