title: GSM7151909: brain; SD; CFC; DG_inf; rep1; Mus musculus; OTHER
library_construction_protocol: Samples were incubated with the following target panels for the Nanostring GeoMx DSP: (v1.0) Mouse NGS Whole Transcriptome Atlas RNA (Lot# MWTA12001). For extraction, regions of interest within the tissue were illuminated with UV light and oligo barcodes were physically aspirated from the tissue and collected into microtiter plates by the GeoMx® Digital Spatial Profiler (DSP) platform. For more information about DSP protocols please see Merritt et al. Nature Biotech 2020 (doi: 10.1038/s41598-020-63539-x). Manufactor's protocol for Nanostring GeoMx DSP with NGS readout; Each collection of oligo tags from one well (representing an ROI from the tissue section) was indexed with i7xi5 unique dual indexes using GeoMx SeqCode primers with 18 cycles of PCR. After PCR, indexed ROIs were pooled and purified prior to sequencing.