title: GSM7147868: PGPC14WT RNAseq; Homo sapiens; miRNA-Seq
library_construction_protocol: For whole cell samples (PGPC14WT, HCA), 1µg was provided The Centre for Applied Genomics, Hospital for Sick Children, Toronto. For ADEV samples, all isolates were provided for bioanalyzer and sequencing. Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB) Small RNA library prep set according to the manufacturer's instructions. Sequencing was performed using Illumina NovaSeq SP flowcell for single end 50bp length reads, aiming for 40M reads per transcript. Library preparation and sequencing were done at TCAG.