title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of 7 breast PDX models treated with Fulvestrant and AZD9833/camizestrant and collected at multiple timepoints
design: RNA-seq of 7 breast PDX models treated with Fulvestrant and AZD9833/camizestrant and collected at multiple timepoints
library_construction_protocol: Samples were harvested in 1 mL of QIAzol lysis buffer and snap frozen.  RNA was extracted using RNeasy 96 QIAcube HT total RNA Cell with DNAse treatment, and samples randomized over 96-well plates. RNA was quantified using the Qubit RNA BR kit (Q10213 Invitrogen), according to the manufacturerâ€™s instructions. The Illumina Truseq stranded mRNA library was generated by the Cancer Research UK Cambridge Institute Genomic core facility