title: GSM7510731: H2AK9ac_Scr_Ctrl_Rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Approximately 10 million cells were then cross-linked with 1% formaldehyde for 10 min at room temperature and quenched with glycine for 5 min. The fixed cells were resuspended in SDS lysis buffer  in the presence of protease inhibitors and subjected to 30 cycles of sonication in ice-cold water to generate chromatin fragments of 300 bp in length. The purified DNA (5 ng of average size 300 bp) was used for library preparation with a VAHTS Universal DNA Library Prep Kit for Illumina V3  and sequenced with PE150 (paired-end, 50 bp reads) on Novogene's NovaSeq high-throughput sequencing platform.