title: GSM7819148: Plate 1 Cell P1_D4; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were then dissociated to single cells by adding 1 ml 1x TrypLE Express directly to each well. Organoids were incubated at 37˚C for 20 minutes with trituration every 5 minutes until a single cell suspension was generated. Cells were stained with ethidium homodimer to exclude dead cells. Single cells were then sorted directly into 96 well PCR plates containing 5 µl NEBNext cell lysis buffer with murine RNase inhibitor and flash frozen on dry ice Libraries were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit manufacturer's protocol