title: GSM8252042: pDC_WT_3; Mus musculus; RNA-Seq
library_construction_protocol: spleens were isolated and digested for 15 and 30 minutes respectively, in the presence of Liberase (50µg/ml, Sigma-Aldrich) and DNAse (250µg/ml, Sigma-Aldrich) in PBS supplemented with 5% FCS (Sigma-aldrich). Organs were then mashed through a 70µm filter and spleens were treated with ACK lysis buffer(BioLegend) for red blood cell elimination. Prepared cell suspensions were then stained with FACS antibodies: cDC1 (Lin-CD11c+MHCII+CD64-XCR1+CD11b-), cDC2 (Lin-CD11c+MHCII+CD64-XCR1-CD11b+) and pDCs (Lin-CD64-CD11cintCD11b-BST2+). Cells were stained and FACS sorted into 500uL of Trizol LS and RNA was then isolated using Qiagen miRNeasy isolation kit following the manufacturer's instructions. RNA libraries were prepared using the SMARTER mRNA-seq library prep kit protocol.