title: 16S rRNA of rumen fluid:con
design: Twelve fistulated peak-lactation dairy cows were selected to be the donor for rumen fluid collection. Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid (FR), 10 L sterile rumen fluid (SR), or 10 L saline (CON) during 36 days after calving. Production performance, plasma indices, plasma lipidome, ruminal microbiome, and liver transcriptome were recorded.