title: GSM8289584: mdx_Freshly_Isolated_MuSC_3; Mus musculus; RNA-Seq
library_construction_protocol: The RNA from in vivo derived MuSCs was isolated using Arcturus Picopure RNA extraction kit (Thermo Fisher). Total RNA from myoblasts and myotubes was extracted using the Nucleospin RNA II kit (Macherey-Nagel). Library construction was performed with 20ng of input total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina - polyA mRNA workflow (New England Biolabs).