title: GSM7734781: WT bud (10 hpf) tRNA-seq rep1; Danio rerio; ncRNA-Seq
library_construction_protocol: 50 whole embryos were collected per sample and immediately lysed in 800uL LiDS/LET buffer(5% Lithium dodecyl sulfate in 20 mM Tris-HCl pH=7.4, 100 mM LiCl, 2 mM EDTA, 5 mM DTT, pH 7.4). tRNA-seq libraries were prepared using the mim-tRNAseq workflow (Behrens 2021, 2022) and sequenced on an Illumina NextSeq 500 platform. mim-tRNAseq