title: GSM7510795: Pus1del_aTC_Biol_Rep1; Saccharomyces cerevisiae SK1; RNA-Seq
library_construction_protocol: Cells were spun down and pellets were flash frozen. RNA extraction was performed as described in Higdon et al. 2023. PolyA selection was performed using NEXTFLEX Poly(A) Beads 2.0 kit with 5ug total RNA (NOVA-512992). RNA-seq libraries were prepared from the resulting poly-A selected RNA using the NEXTFLEX Rapid Directional RNA-Seq Kit 2.0 (NOVA-5198-02).