title: Metagenome: rice soil
design: oligonucleotide primers were designed to anneal to the relatively conserved sequences spanning fungi ITS regions.ITS1 region was amplified using forward primer containing sequence "ACCTGCGGARGGAT" and reverse primer containing sequence "GAGATCCRTTGYTRAA.ITS2 region was amplified using forward primer containing sequence "GTGAATCATCGARTC" and reverse primer containing sequence "TCCTCCGCTTATTGAT".