title: GSM8422168: WT3 AT2 CD44 pos; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were harvested from control and Sftpc mutant lungs, single cells suspension was prepared by mehcanical and enzymatic digetsion.  CD45+ and CD31+ cells were depleted using biotinylated antibodies followed by surface staining with antibody mix to identify CD44+ and CD44- AT2s. AT2 cells were identified as CD45-CD31-Epcam+CD104-CD200hi and then CD44+ or CD44-.. Dead cells were excluded by Dapi staining. Libraries were prepared using Illumina stranded total RNA with Ribo-zero Plus kit accordine to manufacture instructions