title: GSM8402325: Rat, placenta, Saline, Male, Rep 3; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was extracted from male and female placental tissues by homogenizing in TRIzol (ThermoFisher Scientific). After adding chloroform, the aqueous phase was collected and diluted 1:1 with 70% ethanol, placed on RNeasy columns (Qiagen Inc), treated with DNase I, and RNA purified. Libraries were generated using the NEBNext directional library prep kit for Illumina (New England Biolabs) at Génome Québec