title: GSM8249311: RNA_langerhans_cells_BHI_tween_rep4; Mus musculus; RNA-Seq
library_construction_protocol: Langerhans Cells (DAPI- lineage(NK1.1, TCRb, gdTCR)- Ly6G- CD11c+ CD64- CD11b+ CD24+), dendritic cells (DC1: DAPI- lineage(NK1.1, TCRb, gdTCR)- Ly6G- CD11c+ CD64- CD11b- CD24; DC2: DAPI- lineage(NK1.1, TCRb, gdTCR)- Ly6G- CD11c+ CD64- CD11b+ CD24-) and keratinocytes (DAPI- lineage(NK1.1, TCRb, gdTCR)- Ly6G- CD45- CD34- CD49f+ Sca-1+) from the mouse ear of unassociated mice and mice treated with BHI-tween were sorted with a Sony MA900 sorter. RNA was extracted using the Qiagen miRNeasy kit, and sequencing library was prepared using Tecan SoLo total RNA kit for mouse. Libraries were sequenced as 2 x 150bp reads on a Illumina NextSeq 2000 using the P3 200 cycle kit