title: GSM6745948: proximal, WENR-plus, input, biol rep 2; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 12 minutes. After quenching and cell lysis, chromatin was sonicated using a bioruptor (diagenode). DNA extracted from 6 wells of organoids. Chipmentation method (Schmidl et al , 2015; PMID: 26280331) was applied to build the libraries. ChIP-seq libraries were prepared using Kapa HiFi HotStart Ready Mix kit complemented with Nextera XT index kit from Illmina. Post amplification libraries were size selected at 200-500 bp in length using Agencourt AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.