title: GSM8248680: 4wk, AS01, donor3, scATAC; Homo sapiens; ATAC-seq
library_construction_protocol: hNOG mice were euthanized by CO2 inhalation and bone marrow (BM) was collected from both femurs by flushing 10mL of PBS through the bones using a 25G needle. The marrow collected was passed through a 70µm strainer and red blood cells were lysed with 1mL ACK lysis buffer (Gibco) for 5min. Cells were washed with 10mL RPMI containing 2% FBS and centrifuged at 300g for 5min. The resulting cell pellet was resuspended in 1mL PBS and cells were counted using the trypan blue exclusion method. Live human CD45+ cells devoid of mature B, T and NK cells (hCD45+hCD3-hCD19-hCD20-hCD56-) were sorted on the BD FACS Aria IV. hNOG BM single cell suspension of hCD45+hCD3-hCD19-hCD20-hCD56- cells were subjected to nuclei isolation as per 10x Genomics Demonstrated Protocol CG000169. Nuclei were processed by the Chromium Single Cell ATAC Kit (10x Genomics)  v1.1 following the manufacturer's protocol, targeting the capture of up to 6000 nuclei