title: GSM7681466: Mouse, lung, mixed Cre_Neg and Cre_Pos cells, sublibrary 2; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were perfused with PBS and extracted from mice. Lungs were digested in DMEM containing collagenase I (1mg/mL), dispase II (1mg/mL), DNase I (1.25 uU/mL), Aprotinin (20 ug/mL), Pepstatin A (10ug/mL), Leupeptin Hemisulfate (10mg/mL), and PMSF (1 mM). EDTA (5mM) and PMSF (1mM) in DMEM were added to quench the reaction. Single cell suspensions were treated with ACK lysing buffer followed by maganetic bead based depletion of CD45+ cells and enrichment for Epcam+ cells. A cell sorter was used to enrich for live cells based on negative DAPI staining. Parse Evercode Cell Fixation kit v2 and Evercode WT v2 kits were used to prepare cells for sequencing with UDI barcodes.