title: GSM7593526: p300_JMURTK2_Mock_1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed for 15 min at 25°C with 1% formaldehyde. Fixation was stopped by the addition of 125 mM glycine. The fixed cells were washed twice with PBS and chromatin was isolated by addition of lysis buffer, followed by disruption using a Dounce homogenizer. Illumina sequencing libraries were prepared from the ChIP and Input DNAs using the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500, with 75 bp single end reads.