title: GSM7508797: CS23, face, H3K27ac, rep 1; Homo sapiens; ChIP-Seq
library_construction_protocol: Human embryonic face tissue (dissected) was received as de-identified, frozen samples from the Human Developmental Biology Resource (Newcastle University, UK). Frozen tissue was dissociated and crosslinked, cells lyzed and chromatin was sonicated to obtain DNA fragments ranging between 100-600bp. Chromatin was immunoprecipitated with an anti-H3K27ac antibody (Active Motif, Cat#39133), reverse crosslinked  and the samples were assessed for DNA concentration and fragment size distribution. Sequencing libraries were prepared with Illumina TruSeq kit.