title: GSM8026678: HCT116 cell FSTL3 rep1; Homo sapiens; RNA-Seq
library_construction_protocol: The study involved the exogenous addition of humanized FSTL3 protein (MCE) to the culture medium of HCT116 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq after 24 hours. A total amount of 1 ug RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTMRNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.