title: Microbial metagenomics of vineyard soils
design: Following the manufacturer's instructions, total genomic DNA was extracted from approximately 0.25 g soil per sample using the DNeasy PowerSoil Kit (QIAGEN). The isolated DNA is quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen). The bacterial community composition was analyzed by amplifying and sequencing the hypervariable V3-V4 region of the 16S rRNA gene using the primer set Forward (5- TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3)& Reverse (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3). Sequencing was performed on a MiSeq platform following manufacturers guidelines.