title: GSM7030087: rpoS complement in strain HKE9-M-rpoS, rep2; Klebsiella pneumoniae; RNA-Seq
library_construction_protocol: A fresh culture of HKE9, HKE9-M-rpoS, and HKE9-C-M- rpoS strains were grown to OD600 of 0.6. Total RNA was extracted using RNAprotect Bacteria Reagent and RNeasy minikit (Qiagen, DE). RNA quality was monitored on 1% agarose gels. RNA purity (OD260/OD280, OD260/OD230) was checked using the NanoPhotometer® spectrophotometer (Implen, USA). RNA integrity was measured using Bioanalyzer 2100 (Agilent, CA). A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations