title: Analysis of 16S rRNA gene amplicons from nasal swabs of beef calves in Idaho: Comparative Study between Apparently Healthy and Calves Diagnosed with Bovine Respiratory Disease
design: PCR grade water was employed to assemble the 16S rRNA gene library (V4 16S rRNA gene) following the protocol outlined by the Schloss lab and Centeno-Martinez et al., 2022. Subsequently, the 16S rRNA gene amplicons underwent sequencing using Illumina MiSeq (2x250, paired-end) technology.