title: GSM7511296: wild-type regenerating liver, input, replicate 3; Mus musculus; ChIP-Seq
library_construction_protocol: Minced livers were crosslinked in 1% formaldehyde in PBS, and then quenched with 125mM glycine for 10minutes at room temperature. The tissue was then dounced in cold PBS, filtered through a 40uM cell strainer and washed with cold PBS. Chromatin was sonicated to approximatley 500bp, mixed with spike-in chromatin (Active Motif 61686), and incubated with primary antibody (Abcam ab4729) and spike-in anitbody (Active Motifi 61686) overnight at 4C. The mixture was incubated with protein A/G Dynabeads for 3hr at 4C. After washing, chromatin was eluted in 1% SDS buffer, followed by reverse crosslinking at 65C overnight. Pulldown DNA was incubated with RNaseA and proteinase K for 2 hr, and then purified with QIAquick spin column (Qiagen, 28004). ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina Kit (NEB, #E7645S) following the manufacturer's instructions.