title: GSM7883279: OVCA420 cells, 7 day TGFB1 treatment; Homo sapiens; RNA-Seq
library_construction_protocol: OVCA420 cells were washed with PBS then incubated with trypsin for 5 minutes and quenched using normal growth media (DMEM + 10% FBS) . The suspension was filtered, and  large aggregates were further disrupted using a syringe plunger on a 70μm filter in order to generate a single-cell suspension. Cells were counted to assess viability (Trypan-blue exclusion) prior to staining. Single-cell suspensions were processed using the 10x Genomics Single Cell 3' RNA-seq kit (v3), loaded to target a yield of 10,000 cells per sample. Cell cDNA libraries were prepared according to the manufacturer's protocol and libraries were assessed using the Fragment Analyzer (Agilent). 3' scRNA-seq