title: GSM7148186: time_4, ctl, treatment_2, rep_a; Crocosphaera watsonii WH 8501; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Mini Kit (Qiagen Sciences, MD, USA) with β-mercaptoethanol (M6250, Sigma Aldrich, Hamburg, Germany) added to the lysis buffer RLT to enhance intracellular RNAse denaturation (Moisander et al., 2008). Prior to extraction, filters were ground and submitted to five to ten flash freezing steps with liquid nitrogen to maximise the extraction yield. An extra DNAase treatment was performed using the TURBO DNA-freeTM Kit (Ambion, Thermo Fisher Scientific), followed by a Zymo-5 column clean up kit (Zymo Research, USA). RNA libraries for RNA-seq were prepared using total stranded RNA following NovaSeq6000  manufacturer's protocols.