title: Shotgun metagenomics of water: Produced water, absence of reagent, -78 degrees C, 24h, 2.75m high, first biological replica
design: A 450 bp library was prepared from genomic DNA with the NEBNext Fast DNA Fragmentation and Library Preparation Kit (New England Biolabs, Ipswich, NE, USA) following the manufacturers instructions. Library quality was evaluated with Agilent 2100 Bioanalyzer, and shotgun metagenomic sequencing was performed using an Illumina HiSeq 2500