title: prokaryotic (16S) metagenome
design: 16S rRNA gene amplicons were prepared and sequenced using 300 bp paired-end read Illumina MiSeq V3 sequencing by LGC Genomics (Berlin, Germany). Using the primer pair 515YF (GTG YCA GCM GCC GCG GTA A)/B806R (GGA CTA CNV GGG TWT CTA AT)