title: GSM7328793: Liver, Naïve; Mus musculus; RNA-Seq
library_construction_protocol: Freshly dissected livers were minced finely and incubated in digestion buffer (1mg/ml collgenase P in Ca2+/Mg2+ containing HBSS) in a shaking incubator at 37 degrees for 30 minutes. Cell extract was further digested with 0.25% trypsin-EDTA at 37 degrees for 5 minutes. After removal of RBCs, cells were then resuspended in PBS + 0.5% BSA and stained with viability dye and CD45, CD31,  and Epcam antibodies. Live, CD45-, CD31-, Epcam-, GFP+ cells were then isolated using FACS. Library construction was performed using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit v3.1, according to the manufacter's instructions