title: GSM7883661: F14630-L1_S75_L008_001 Colon AVd; Mus musculus; RNA-Seq
library_construction_protocol: RNA of colonic IECs was isolated according to the manufacturer's instructions (NucleoSpin RNAII kit, Macherey-Nagel) and measured by NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific). TruSeq stranded Total RNA kit (Illumina Inc) was used for the construction of strand-specific sequencing libraries from the isolated colonic IEC RNA.