title: Whole genome sequencing analysis of MDR Pseudomonas aeruginosa isolated from liver transplant patient
design: DNA library was prepared using TruSeq Nano DNA Kit. High molecular weight isolated DNA was tagmented followed by short cycle of PCR. The amplified product was purified and processed for evaluation. Final library was denatured and diluted with HT1 buffer. The library was subjected for clustering and sequencing on the HiSeq illumina platform