title: WGS of human Multiple Myeloma: Peripheral blood mononuclear cells: Sample CTF056_WBCs
design: Sorted samples underwent DNA purification (Thermo Fisher PicoPure DNA Isolation Kit). Library preparation used the NEBNext Ultra II FS DNA Library Prep kit (New England Biolabs). Final library fragment sizes were assessed using the BioAnalyzer 2100 (Agilent Technologies), with yields being quantified by Qubit 3.0 fluorometer (Thermo Fisher Scientific) and qPCR (KAPA Library Quantification Kit). Final sample libraries were normalized and pooled, before whole genome sequencing was performed on Illumina Novaseq6000 S4 flowcells, 300 cycles paired-end reads, at the Genomics Platform of the Broad Institute of MIT and Harvard.