title: RNA-seq of coral-associated bacteria
design: The extracted DNA were assayed for concentration using a Multiskan SkyHigh Microplate Spectrophotometer. PCR amplification using 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) as primers for the V3V4 variable region of bacterial 16S rRNA was performed with an ABI GeneAmp 9700 thermal cycler, ollowed by detection and quantification of PCR products. The TruSeqTM DNA Sample Prep Kit (Illumina lnc., California, USA) was used to prepare Illumina libraries, which were then sequenced in the Illumina MiSeq platform following standard protocols.