title: GSM7434926: SOW004_T47D_4h9363_2; Homo sapiens; OTHER
library_construction_protocol: In brief, bromouridine was added into live cell culture at treatment end points to a final concentration of 2 mM and incubated at 37 degree for 30 minutes. Total RNA was extracted with Trizol reagent (Invitrogen #15596-018) and BrU-RNA isolation, library preparation, sequencing and data analysis were performed as described previously (Paulsen et al., 2014).