title: GSM7510990: Liver, ORX_Nx, mice 1; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extraction from frozen liver samples using EasyPure® RNA Kit, Libraries were generated from 250 ng of total RNA mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were done using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs.