title: GSM8114945: CCCR_WC_ncRNA-seq2; Homo sapiens; ncRNA-Seq
library_construction_protocol: Trizol extraction was completed according to manufacturers protocol. RNA libraries were generated using the NEXTFlex Small RNA Library Preparation Kits v3 (Perkin) with the following modifications: (1) 3′- and 5′-adaptors were diluted 1:8, (2) 3′-adaptor ligations were performed overnight in multiple steps –25°C for 2 h, 20°C for 4 h and 16°C overnight, (3) following cDNA synthesis and before barcoding PCR, step F protocol was followed, and (4) PCR amplification was 20 cycles.  Following PCR amplification, individual libraries were size-selected (136–200 bp product) using Pippin Prep (Sage Sciences).  Size-selected libraries were quantified using Qubit Fluorometer.  Libraries were checked for quality and sequenced using Illumina short-read technology.  Libraries were pooled (equimolar multiplexed libraries) and sequenced (PE150) using the NovaSeq6000 (Illumina) platform at the VANTAGE Core (Vanderbilt University).