title: GSM7029810: BM progenitor cells, mRNA-derived cDNA [AsanoWTA1]; Mus musculus; RNA-Seq
library_construction_protocol: BM cells from one naïve mouse and one LPS-injected mouse were sorted by FACSAriaIII and stained with different DNA barcode-conjugated PE-streptavidins (BioLegend Totalseq PE-streptavidin (A961, A962, A963, A964, and A965)) to identify each sample origin. Sorted cells were pooled and subject to scRNA-seq using BD RhapsodyTM Single-Cell Analysis System (BD  Biosciences), and resulted cDNA was amplified by TAS-Seq protocol. 100 cells /A961 ; 100 cells /A962 ; 15,000 cells /A963 ; 100 cells /A964 ; 15,000 cells /A965 were loaded onto BD RhapsodyTM Single-Cell Analysis System. cDNA library was processed to sequencing library by using NEBNext UltraII FS library prep kit for Illumina (New England Biolabs). Sequencing adapters were added to associated Totalseq PE-streptavidin libraries by PCR. Pooled library concentration was adjusted to 2.0 nM and 12% PhiX control library v3 (Illumina) was spiked into the library.