title: GSM8027625: Donor_1_uns_IMDM; Homo sapiens; RNA-Seq
library_construction_protocol: At the end of differentiation, monocytes were isolated using pan monocytes kit (MACS). The monocytes obtained were then stimulated for 4 hours with IMDM medium as control or LPS 10 ng/ml for 4 hours and cells were stored in RNAlater buffer. Total RNA were extracted using the QIAGEN AllPrep DNA/RNA micro-kit according to manufacturer's instructions. Library preparation was performed using the Illumina TruSeq stranded mRNA kit with a starting input of 300ng and libraries were sequenced on the IlluminaNovaSeq 6000 instrument generating an average of 20 million reads per sample.