title: GSM7508787: CS18, face, ATAC, rep 2; Homo sapiens; ATAC-seq
library_construction_protocol: Human embryonic face tissue (dissected) was received as de-identified, frozen samples from the Human Developmental Biology Resource (Newcastle University, UK). Tissues were lysed, centrifuged for 10min at 500 x g, at 4 degree C, and the resulting cell pellet was treated with the Nextera DNA transposase Tagment DNA Enzyme (Catalog number: 20018705) and the transposed DNA was eluted using Qiagen MinElute PCR purification kit. Samples were PCR amplified using the NEB Next High-Fidelity 2xPCR Master Mix (Catalog number: NEBE6040SEA) with Nextera PCR primers, and DNA was purified. The eluted library was analyzed for quality in a Bioanalyzer High Sensitivity Assay and samples were subsequently deep sequenced on an Illumina HiSeq2500.