title: GSM7030311: TBP mice, control, scRNA; Mus musculus; RNA-Seq
library_construction_protocol: The Chromium Single Cell 3′ Library & Gel Bead Kit v2 (PN120237), Chromium Single Cell 3' Chip Kit v2 (PN-120236), and Chromium i7 Multiplex Kit (PN-120262) were used according to the manufacturer's instructions in the Chromium Single Cell 3′ Reagents Kits v2 User Guide. The single-cell suspension was washed twice with 1× phosphate-buffered saline (PBS) + 0.04% BSA. Cell number and concentration were confirmed with TC20 Automated Cell Counter. Cells were subjected immediately onto a 10× Genomics Chromium Controller machine for gel beads-in-emulsion (GEM) generation. Barcoded complementary DNAs (cDNAs) were prepared using a 10× Genomics Chromium Single Cell 3′ reagent kit (V2 chemistry), which was subsequently recovered, purified, and amplified to generate sufficient quantities for library preparation. Library quality and concentration were assessed using Agilent Bioanalyzer 2100. Libraries were run on the NovaSeq platform of Illumina for PE150 sequencing.