title: BGISEQ-500 paired end sequencing; RNA-seq of Arabidopsis thaliana spms and wild-type plants inoculated with Pst DC3000 or mock
design: RNA-seq of Arabidopsis thaliana spms and wild-type plants inoculated with Pst DC3000 or mock
library_construction_protocol: Leaf infiltration was conducted on 5-week-old Arabidopsis plants using a 1-ml needleless syringe. The infiltration was performed on fully expanded rosette leaves. Leaves at the same developmental stage were chosen for infiltration. At 24 h post-inoculation, only the infiltrated leaves were collected Seeds of the different genotypes were sown on a soil mixture containing peat moss (40%), vermiculite (50%) and perlite (10%), and stratified in the dark at 4°C for 2-3 days. Plants were grown at 20–22℃ under 12-hour light/ 12-hour dark photoperiod cycles. Pst DC3000 (OD600 =0.001) and mock (10 mM MgCl2) inoculation treatments were performed in three biological replicates using three different plants per genotype and treatment. Total RNA was extracted using TriZol (Thermo Fisher) and further purified using RNeasy kit (Qiagen) according to manufacturer's instructions. Libraries were prepared using the MGIEasy RNA Library Prep kit (MGI Tech) according to manufacturer's instructions