title: GSM8195523: Timecourse, sublibrary 8; Mus musculus; RNA-Seq
library_construction_protocol: Cells cultured on hydrogels were washed with PBS and detached from the substrates by treatment with TrypLE Express and manual pipetting. The cells were collected by centrifugation and fixed using the Cell Fixation kit from Parse Biosciences. Single cell libraries for RNA sequencing were prepared based on a SPLiT-seq approach using the Evercode Mini WT and Evercode WT kits from Parse Biosciences, following the manufacturer's protocols. Library quality was validated using an Agilent Bioanalyzer 2100 at the Stanford Genomics Facility.