title: Horse metagenome 38 biological replicate 2
design: DNA from fecal microbiota were collected from healthy Buryatian horses grazing cold-season (November, meteorological winter) grass pastures. The amplicon libraries of the hypervariable V4 region ofthe 16S rRNA gene were prepared using the two-stage PCR strategy with the primers 515F and Pro-mod-805R. PCR of every DNA sample was carried out in duplicates, the detailed amplification protocol is described previously