title: GSM8422158: Sftpc_WT_1; Mus musculus; RNA-Seq
library_construction_protocol: A single cell suspension was prepared by physical and enzymatic dissociation (Collagenase, DNase, Dispase) followed by MACS sorting by LS columns with CD45+ and CD31 cell removal using CD45 and CD31 micobeads followed by EPCAM+ cell enrichment using EPCAM microbeads. CD45-/CD31-/EPCAM- cells were considered lung mesenchymal cells. EPCAM+ and mesenchymal cells were prepared in 50/50 concentration for sequencing. Libraries were prepared using Illumina stranded total RNA with Ribo-zero Plus kit accordine to manufacture instructions