title: GSM7225651: IDD-rep 3; Rattus norvegicus; miRNA-Seq
library_construction_protocol: Total RNA was extracted from the nucleus pulposus tissue of each sample. Total RNA of each sample was used to prepare the miRNA sequencing library, which included the following steps: 1) 3'-adaptor ligation; 2) 5'-adaptor ligation; 3) cDNA synthesis; 4) PCR amplification; 5) Size selection of ~150 bp PCR amplicons (corresponding to ~22nt miRNAs). The libraries were denatured as single-stranded DNA molecules, captured on Illumina flow cells, amplified in situ as clusters and finally sequenced for 50 cycles on Illumina Novaseq 6000 sequencer following the manufacturer's instructions.