title: GSM6919218: CD3-53, Het, iNeuron; Homo sapiens; RNA-Seq
library_construction_protocol: All RNA samples were extracted with Trizol reagent according to the manufacturer's instruction (Invitrogen). SCN2A RNAseq libraries were prepared from 200 ng of total RNA using a TruSeq stranded mRNA Sample Prep Kit (Illumina cat# RS-122-2102). Libraries were multiplexed, pooled and sequenced on multiple lanes of the Illumina NovaSeq platform, generating an average of 30.7M paired-end 150 bp-cycle reads for 30 samples (20 SCN2A heterozygous deletion, 10 SCN2A wild-types)