title: GSM8248707: IPSCs, control, Hi-C; Homo sapiens; Hi-C
library_construction_protocol: Genomic DNA of cells was crosslinked in situ using serum-free DMEM with 2% formaldehyde and then were flash-frozen in liquid nitrogen before digested. The crosslinked cells were lysed and digested with MboI restriction enzyme. After biotinylated and ligated, the fragments were purified and assessed for quality. The qualified samples were reverse crosslinked and then submitted for library constructions. Standard library constructions were performed, including sonication fragment, end repair, A-tailing, biotin enrichment, adapter ligation, and amplification.