title: GSM7818659: HuH-7 cells, FOXA1; Homo sapiens; ChIP-Seq
library_construction_protocol: HuH-7 cells were cross-linked with 1% formaldehyde for 10 min at room temperature, and 0.1 M glycine was added to quench the formaldehyde. Chromatin extracts were sheared by micrococcal nuclease digestion (New England Biolabs), and protein-DNA complexes were immunoprecipitated with 5 μg of anti-CEBPA (PA5-77911, Thermo Fisher Scientific) or anti-FOXA1 antibody (17-10267, Merck) bound to Dynabeads Protein G (Thermo Fisher Scientific). ChIP-seq libraries were prepared using an NEBNext Ultra II DNA Library Prep kit (New England Biolabs) according to manufacturer's instructions. 1 ng of DNA was used for the construction of sequencing libraries. 2 × 150 bp