title: GSM7508879: face_hs746_e11.5_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Embryonic mouse face tissue was dissected, embryos with positive mCherry reporter activity wwre selected where applicable, and immediately processed for downstream experiments. Fresh mouse embryonic face tissue was mechanically dissociated by pipetting gently into a single-cell suspension using Accumax, assessed for viability of cells and cell density using Trypan Blue staining. Tha standard protocol for 10X Genomics Chromium Next GEM Single Cell 3' was followed for transcript capture and library preparation for single-cell gene expression. Samples were either processed individually or pooled using a previously described Multi-seq strategy78 upstream of the 10X Genomics Chromium protocol. The resulting libraries were sequenced on an Illumina HiSeq2500 or NovaSeq 10X. 10X Genomics Chromium Next GEM Single Cell 3'