title: GSM7148150: ONC_cKO; Mus musculus; Bisulfite-Seq
library_construction_protocol: DNA was harvested using Quick-DNA MiNIprep Kit (Zymo). 200 ng of total DNA was used for the construction of sequencing libraries. Firstly, the genomic DNA spiked with lambda DNA were fragmented to 200-400 bp. The DNA fragments were bisulfite treated to generate single strand DNA (ssDNA). During the bisulfite conversion, unmethylated cytosine were converted into uracil, while methylated cytosine staid unchanged. Methylation sequencing adapters were ligated, followed by double strand DNA synthesis. The library was ready after size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.