title: RNA-seq of patients with aplastic anemia in the presence or absence of levamisole in vitro
design: RNA-seq of patients with aplastic anemia in the presence or absence of levamisole in vitro
library_construction_protocol: BM nucleated cells (BMNCs) from healthy donors and patients with NSAA at the time of initial diagnosis were separated, and then co-cultured with or without 40 μg/mL LMS for 48 hours in vitro. Cells were harvested and total RNA were extracted.  Total RNA after different treatments was extracted using Trizol as described by the manufacturer's protocol. The assay were carried out using the Illumina HiSeq X platform and the data obtained were analyzed by following the Hisat2 protocol.