title: GSM7883021: DMSO_i2C_1; Mus musculus; RNA-Seq
library_construction_protocol: Cellular RNA was collected using the FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme, cat. no. RC112).RNA of embryos was extracted by the PicoPure RNA Extraction Kit (Arcturus, cat. no. KIT0204). For the RNA-seq library of mESCs, Total RNA was used as input material for the RNA sample preparations. First-strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, then use RNase H to degrade the RNA. Second-strand cDNA synthesis was subsequently performed using DNA Polymerase I and dNTP. The library fragments were purified with the AMPure XP system (Beckman Coulter, Beverly, USA) for preferentially cDNA fragments. After PCR amplification, the PCR product was purified by AMPure XP beads, and the library was finally obtained. For the RNA-seq library of embryos, 10-15 4-cell stage embryos were pooled per sample. The total RNA of each sample was extracted by the PicoPure RNA Extraction Kit (Arcturus, cat. no. KIT0204). 200 ng total RNA was used for library generation using SMARTer Stranded Total RNA-Seq Kit v2 (Takara, cat. no. 634836).