title: GSM7030647: A549, SP 3 Hours, R2 (ChIP-seq); Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were double-crosslinked in 2 mM DSG and 1% Formaldehyde. After fixation, cells pellet was sonicated until fragment size ranges between 200 bp and 500 bp. Sonicated chromatin was then immunoprecipitated overnight using Dynabeads protein A (Dynabeads, 10002D) coated with the antibody of interest. Beads were washed, and the DNA was decrosslinked and purified using standard phenol-chloroform purification. Sequencing libraries was constructed with the purified DNA using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, E765), and sequenced with NovaSeq 6000 (Illumina) platform.