title: GSM7883420: rnaseq_wt_42; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen). RNA sequencing libraries were prepared using Roche Kapa mRNA HyperPrep sample preparation kits from 100ng of purified total RNA according to the manufacturer's protocol. The finished dsDNA libraries were quantified by Qubit fluorometer, Agilent TapeStation 2200, and RT-qPCR using the Kapa Biosystems library quantification kit according to manufacturer's protocols. Uniquely indexed libraries were pooled in equimolar ratios and sequenced on Illumina NovaSeq 6000 with paired-end 150bp reads