title: GSM6746057: V08-3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from whole blood samples was isolated using a PAX gene Blood RNA kit (PreAnalytix) according to the manufacturer's instructions. The globin RNA was removed from total RNA using a GLOBINclear-Human Kit (Thermo Fisher SCIENTIFIC). For RNA sequencing, 500 ng total RNA were rRNA-depleted using the NEBNext rRNA Depletion Kit (New England Biolabs) according to the manufacturer's instructions. Library preparation was performed using an NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs).