title: RNA-Seq of spodopteralitura: larvae
design: To analyze the adaptation of Spodoptera litura to low-temperature environments, the third instar S. litura larvae were kept at 0  for 8 hours. RNAs were isolated  and also from control larvae (28 ). The obtained transcriptome from the S.litura was set for illumina strand specific RNA sequencing (RNA-seq) to analyze changes of gene expression subject to cold stress.