title: GSM6918898: MDP1_#1 cKD  Day7  biol rep 2; Mycobacterium tuberculosis variant bovis; RNA-Seq
library_construction_protocol: RNA was harvested using Direct-Zol RNA MiniPrep kit (Zymo research) following manufacturer's protocol cDNA sample was randomly fragmented followed by 5' and 3' adapta ligation. Alternatively  "tagmentation" was done to combine the fragmentation and ligation in a single more efficient step. Adapter-ligated fragments were then PCR amplified and gel purified