title: GSM7147998: scRNA_somitic_gastruloids_Zeb2_dTAG13; Mus musculus; RNA-Seq
library_construction_protocol: Gastruloids (~32) were harvested from matrigel with icecold PBS and spun down at 300xg for 5 min at 4°C. Gastruloids were trypsinized for 5 min at 37°C and resuspended in DMEM supplemented with 15% FBS. Cells were washed with cold PBS and resuspended in PBS containing 7-AAD and 0.04% BSA. Live cells were sorted at 4°C with a BD FACSMelody™ cell sorter in DNA LoBind tubes containing PBS with 0.04% BSA and centrifuged at 300xg for 5 min. Per sample, 10,000 cells were processed using the 10x Genomics Chromium Controller and the Chromium Single Cell 3' Reagent kit (v3.1 Chemistry) following the standard manufacturer's protocol. ingle cell RNA sequencing libraries were generated using the Chromium Single Cell 3' Reagent kit (v3.1 Chemistry) following the standard manufacturer's protocol (10x genomics). The resulting libraries were sequenced on an Illumina Nextseq500 (1x 28bp + 1x56bp + 1x8bp index).