title: GSM8196352: MZ_Cotwin2_clone2; Homo sapiens; RNA-Seq
library_construction_protocol: We have prepared the neurons for RNA-seq according to apublished FACS protocol.Briefly, five- week-old neurons weredissociated and CD184−/CD44−/CD15-/CD24+cells (Miltenyi Biotec,cat. number 130-103-868, 130-113-334, 560828, 130-099-399, respectively) were collected and subjected to StrandedmRNA (PolyA+)-SeqLibraryPrep. Atotalof1000ng ofRNAwasusedforlibrarypreparation using the Illumina TruSeq RNA Sample Preparation Kit. The libraries weresequenced on Illumina HiSeq2500 with 50 bp single-end reads.