title: GSM7790935: input_ChIP-seq_myl7:GFP+ CMs; Danio rerio; ChIP-Seq
library_construction_protocol: scRNA-seq: FACS sorted for GFP+ cells from wt and hand2 mutant embryos; ATAC-seq and CHIP-seq: Sorted GFP positive and GFP negative nuclei were washed and lysed in 1 ml of water and spun at 12 000 g for 20 minutes. scRNA-seq: Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).  ATAC-seq:The DNA was then purified using the Qiagen Minelute column.  Both samples were amplified for 14 cycles as described in Buenostro 2013 ( PMID: 24097267).    CHIP-seq: The library was prepared using NEXTflex qRNA-Seq Kit v2 (BioScientific #5130-12).