title: RNA-seq of male F1: CC019/TauUnc x CC037/TauUnc liver
design: Strand-specific mRNA libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs #E7760), and mRNA isolation was performed using the Poly(A) mRNA Magnetic Isolation Module (New England BioLabs #E7490). The stranded mRNA libraries were sequenced on an Illumina NovaSeq 6000 using v1.5 chemistry with S4 flow cell, generating 150 bp paired-end dual-indexed reads.