title: GSM6917523: ChIP PH, Control no ph-KD, rep2; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: ChIP-Seq on 3rd instar imaginal eye discs were essentially performed as described in Loubiere, Delest et al., Nature Genetics (2016), with minor modifications. 400 eye discs were used per replicate. When necessary, several batches of dissection/collection were snap frozen in liquid nitrogen and stored at âˆ'80Â°C to collect enough material. Chromatin was sonicated using a Bioruptor Pico (Diagenode) for 10 min (30sec in, 30sec off). PH antibodies were diluted 1:100 for IP. After decrosslinking, DNA was purified using MicroChIP DiaPure columns from Diagenode. DNA libraries for sequencing were prepared using the NEBNextÂ® Ultraâ„¢ II DNA Library Prep Kit for Illumina. Sequencing (paired-end sequencing 150bp, approx. 4Gb/sample) was performed by Novogene (https://en.novogene.com/).