title: GSM7683185: sample a1 (WT, isotype-treated, replicate 1) GEX; Mus musculus; RNA-Seq
library_construction_protocol: B16 tumors were resected from mice and mechanically minced prior to treatment with collagenase and DNAse for 10 minutes at 37C. The cells were washed and filtered through 70 micron filters to obtain single cell suspensions, then labeled with CD45+ MACS beads per the manufacturers instructions. CD45+ cells were isolated on LS columns by positive selection. Cells from each mouse were counted, labeled with hashtag antibodies, and pooled together per experimental group. Pooled cells from each experimental group were loaded onto a single lane of the 10x Chromium controller at the appropriate cell number needed for a calculated post-deconvolution doublet rate of less than 5%. Library construction was performed according to the manufacter's instructions (single cell 3' v3 protocol with feature barcoding, 10x Genomics).