title: GSM7791212: platelet, PSL, rep2; Homo sapiens; miRNA-Seq
library_construction_protocol: (1) Agarose gel electrophoresis analysis of RNA degradation and whether there is pollution;(2) Nanodrop detection of RNA purity (OD260/280 ratio);(3) Qubit for precise quantification of RNA concentration;(4) Agilent 2100 Accurate Detection of RNA Integrity After passing the sample detection, a library was constructed using the Small RNA Sample Pre Kit. The special structures at the 3 'and 5' ends of Small RNA were utilized (with complete phosphate groups at the 5 'end and hydroxyl groups at the 3' end), and total RNA was used as the starting sample. Two ends of Small RNA were directly connected with connectors, and then reverse transcribed into cDNA. Subsequently, after PCR amplification, the target DNA fragment was separated by PAGE gel electrophoresis, and the cDNA library was obtained by gel cutting and recycling.