title: GSM7683952: adult cardiomyocyte, tarmoxifen treatment,3day; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell sequencing library was generated using the iCell8 platform. In brief, isolated cells were stained with a mixture of Hoechst 33342 and propidium iodide (Thermo Scientific, R37610) according to the manufacturer's instruction. After staining, cells were washed with PBS, and counted on a Moxi TM Automated Cell Counter. Afterwards, a cell suspension of 20,000 cells/ml was submitted to the Multi Sample Nano Dispenser (MSND, Wafergen Biosystems) for single cell preparation. The dispensed cells were then imaged on the Imaging Station, and single live cells, defined by Hoechst-positive and propidium iodide-negative staining, were selected. Selected cells were subjected to reverse transcription and first-step amplification on a Chip Cycler (Bio-Rad), and the resulting cDNA was purified and size-selected with Agencourt AMPure XP beads (Beckman Coulter, A63880). One nanogram of purified cDNA was applied to generate a sequencing library using Nextera XT DNA sample preparation Kit (Illumina, FC-131-1024). Libraries were sequenced on the NovaSeq 6000 (Illumina) using paired-end sequencing protocol.