title: Oct18_1
design: Purified Poly (A) mRNA was fragmented into short lengths. cDNA was synthesized using random primers, and following by purifying and end-reparation, and poly(A) tails addition, then ligated to Illumina sequencing adapters. Generated fragments were further purified by agarose gel electrophoresis and then enriched by PCR amplification, and were sequenced.