title: GSM7778669: DRG 3; Homo sapiens; OTHER
library_construction_protocol: Samples were immediately cleaned from the blood and connective tissue and frozen on dry ice.The whole ganglia were embedded in OCT for spatial transcriptomics.Cryosections were cut at 10-μm thickness and mounted onto the GEX arrays. Sections were placed on a Thermocycler Adaptor with the active surface facing up and were incubated at 37°C for 1 min. Then, sections were fixed with methyl alcohol at -20°C for 30 min, followed by staining with hematoxylin and eosin (H&E) (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702). Visum spatial libraries were constructed using Visum spatial Library construction kit (10x Genomics, PN-1000184) according to the manufacture's introduction.