title: GSM7510130: wt_lib_3; Harpegnathos saltator; RNA-Seq
library_construction_protocol: Antennae were frozen and ground with a pestle, then Dounce-homogenized in homogenization buffer (250 mM sucrose, 10 mM Tris pH 8.0, 25 mM KCl, 5 mM MgCl2, 0.1% Triton X-100, 0.5 mM RNAseOUT, 1X Promega protease inhibitor, 0.1 mM DTT), filtered, washed and resuspended in 1xPBS+1%BSA+1mM RNAseOUT, then filtered again We used 10X Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 (single-index version for wt_lib_1 and dual-index version for the remaining libraries) and folllowed manufacturer's instructions.