title: GSM6456125: 12mo_HSC_MHCII_hi_rep3; Mus musculus; RNA-Seq
library_construction_protocol: MHCII-hi and MHCII-lo HSCs were sorted from the BM of WT mice at 12 mo.RNA was extracted from sorted cells using a RNeasy Micro Kit (QIAGEN) following the manufacturer's procedure. The RNA amount and purity were quantified using NanoDrop ND-1000 (NanoDrop). Libraries for RNA-seq were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.