title: GSM7791927: MKN45 cells, IP-si1-1; Homo sapiens; RIP-Seq
library_construction_protocol: polyadenylated RNA was extracted using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, UK) and then fragmented into ~ 150 nt fragments using magnesium RNA fragmentation buffer (New England Biolabs).  Fragmented polyadenylated RNA (5%) was used as the input sample, and the remaining samples were conducted to m6A-RIP. Library construction was performed using the NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer's instructions. m6A-RIP seq