title: GSM7819096: W3_Winnie_Males; Mus musculus; RNA-Seq
library_construction_protocol: TRIzolTM (Invitrogen, VIC Australia) was used to extract total RNA, which was purified using the RNeasy® Mini kit (Qiagen, Hilden, Germany), including column DNase treatment. The RNA-Seq library was formulated by starting with >100 ng of RIN 8+ total RNA, including RNA quality control (CE integrity analysis by AATI Fragment Analyser, Fluorimetric quantitation by Invitrogen Qubit). The Poly-A mRNA was chosen, and MGIEasy stranded mRNA chemistry was carried out. All samples were quantified and analysed using Qubit and Bioanalyser and passedthe QNAQC standard.