title: GSM8249426: Liver_AL_CT28B; Mus musculus; RNA-Seq
library_construction_protocol: RNA from tissues was homogenized in 1mL of TRIzol and extracted according to manufacturer instructions. All RNA samples with RIN >7.5 were used; 260/280 ratio > 2; and 260/230 ratio > 2. mRNA-Seq (polyA selection); mRNA-seq library preparation was done according to Illumina single-stranded mRNA-seq. Briefly, library was prepared with 25 ng of mRNA polyA-selected from 5ug total RNA, with 13 cycles of PCR amplification. Library size was confirmed by Tapestation 2200 (Agilent).