title: RNA Seq 1
design: The details of Sequencing identifier of Illumina are as follows: (1) HWI-ST1276:71 HWI-ST1276, Instrument - unique identifier of the sequencer; 71, run number - Run number on instrument. (2) C1162ACXX:1:1101:1208:2458 means the coordinate of read on C1162ACXX (Flowcell ID) flowcell, line 1, 1101 tile is(x=1208, y=2458). (3) 1:N:0:CGATGT the first number is 1 or 2, 1 means single reads or the first read of paired ends, 2 means the second of paired ends; the second letter means whether reads is adjusted(Y means yes, N means no); the third number represent the number of Control Bits in sequence; six bases on the fourth place is Illumina index sequence.