title: GSM8027864: N17_PRE; Homo sapiens; RNA-Seq
library_construction_protocol: Whole blood total RNA was extracted using the PAXgene blood RNA extraction kit (Qiagen, Hilden, Germany), according to the manufacturer's instructions. Extracted RNA samples were quality checked using the Agilent 2100 Bioanalyzer platform (Agilent Technologies, Santa Clara, USA). cDNA libraries were prepared for sequencing using strand specific TrueSeq kits, with ribosomal RNA and globin mRNA depletion. At least 30 million 150 bp paired-end reads were generated per sample using Illumina NovaSeq 6000 (Illumina, San Diego, USA), employing standard protocols at the Victoria Clinical Genetics Services facility (Melbourne, Australia).