title: GSM7065132: iOL KO H3K27ac-2; Mus musculus; ChIP-Seq
library_construction_protocol: Briefly, cells were crosslinked with 0.9% formaldehyde for 9 min at room temperature, and formaldehyde was quenched by glycine at a final concentration of 125 μM for 5 minutes. Cells were washed twice with ice-cold PBS and collected by a cell scraper. Cell pellets were lysed and sonicated in lysis buffer (1% SDS, 50 mM Tris-HCl (pH 7.4), 10 mM EDTA), and the chromatin was fragmented into 200-500 bp fragments using a COVARIS S220 sonicator. ChIP-seq libraries were prepared using a commercial kit (Vazyme, Nanjing, China, ND607)  following manufacturer's protocols.