title: DNA-Seq of Hemicentrotus pulcherrimus: gonad
design: Gonad tissues were preserved in 95% EtOH and total DNA extracted. A 6 ng aliquot of genomic DNA from a single individual was fragmented using a BioRuptor (Diagenode Inc., Denville, NJ) using six cycles of 30s on/off at the High setting for 5 min. Paired-end libraries were prepared from the fragmented genomic DNA with mean insert sizes of 200  300 bp.