title: WGS (illumina) of Anopheles coluzzii: adult mosquitoes whole body
design: DNA was extracted from individual L4 following a protocol modified from Collins et al. (1987). Briefly, each larva was ground in 200l CTAB buffer (100mM Tris HCL, pH8.0, 10mM EDTA,1.4M NaCl, 2% CTAB), then incubated for 15 minutes at 60C. 200l of chloroform with 4% of isoanyl alcohol were added, and the solution was centrifuged for 10 minutes at 8000 rotations/min. The supernatant was transferred to a new tube with 200l of isopropanol to precipitate DNA at room temperature. DNA was washed with 400 l of 70% ethanol after 10 minutes of centrifugation (10000 rotations/min), dried, and then rehydrated in 50l H2O.