title: GSM8422446: 5XFAD without the MR1/MAIT cell axis Both Male and Female; Mus musculus; RNA-Seq
library_construction_protocol: Brains were removed and hippocampi were sub-dissected and flash-frozen in dry ice, then stored at -80°C until the experiment. The 10x Genomics Chromium Nuclei Isolation Kit (PN-1000494, 10X Genomics, Pleasanton, CA) with 20mg of hippocampus. The single nuclei analysis was performed using the 10X Chromium single cell system (10X Genomics) and the NovaSeq 6000 sequencer (Illumina, San Diego, CA). The final nuclei concentration of 700-1200/µL was chosen for targeted nuclei recovery of 10,000. Following the CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D (10X Genomics), nuclei suspension along with RT reagents, and the single-cell multiome gel beads and partition oil, was transferred onto a Next GEM Chip G in separate wells and the chip was loaded to the Chromium X Controller for GEM generation and barcoded, followed by RT reaction then cDNA synthesis and cDNA library preparation. The resulting cDNA libraries were sequenced separately, cDNA library for 28bp and 91bp paired end was sequenced on Illumina NovaSeq 6000.