title: 16s rDNA of mice
design: Total microbial DNA was extracted using the kits (Tiangen Biotechnology, Shanghai, China). The integrity, concentration, and purity of DNA were detected before sequencing on the Illumina NovaSeq 6000 platform (Majorbio, Shanghai). Illumina PE300 reads were obtained by sequencing the V3-V4 region of the 16S rRNA gene using primer pair 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTAC HVGGGTW TCTAAT-3') by an ABI GeneAmp? 9700 PCR thermocycler (ABI, CA, USA)