title: GSM7883483: WT1; Danio rerio; RNA-Seq
library_construction_protocol: The sample RNA was extracted and purified using the Qiagen kit 1Select the corresponding testing methods for quality inspection according to the requirements of samples and products.                                                                            2 mRNA Isolation 3 mRNA Fragmentation 4 cDNA Synthesis 5 End Repair, Add A and Adaptor Ligation 6 The PCR reaction system and program are configured and set up to amplify the product. 7 Library QC 8 Circularization Single-stranded PCR products are produced via denaturation. The reaction system and program for circularization are subsequently configured and set up. Single-stranded cyclized products are produced, while uncyclized linear DNA molecules are digested. 9 Sequencing