title: GSM8196282: Ri01_dis, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: A Chromium Next GEM Chip N (10X genomics) was loaded with the appropriate number of cells, and the sequencing libraries prepared with the Chromium Next GEM Single Cell 5' HT Reagent Kits v2 dual index following the manufacturer's recommendations. Briefly, an emulsion encapsulating single cells, reverse transcription reagents and cell barcoding oligonucleotides was generated. After the actual reverse transcription step, the emulsion was broken, and double stranded cDNA generated and amplified in a bulk reaction. For V(D)J sequencing, a similar approach was followed except that 2 steps of PCR based V(D)J target enrichment were performed prior to fragmentation. This cDNA was fragmented, a P7 sequencing adaptor ligated, and a 5' gene expression library generated by PCR amplification.