title: Biodiversity in Wetland + system: A passive solution for HCH dump effluents Biodiversity
design: Microbial abundance was assessed by amplifying the V4 region of the bacterial 16S rDNA gene using the primers 530F (5TGCCAGCMGCNGCGG3) and 802R (5TACNVGGGTATCTAATCC3) in a final volume of 50 microL. The amplicons were cleaned using the Agencourt Ampure XP system (Beckman Coulter, USA) and the Ion Torrent platform (Thermo Fisher Scientific, USA) was used for sequencing analysis. Barcoded sequencing adapters were ligated to the PCR products using the Ion Xpress Plus gDNA fragment library kit with Ion Xpress barcode adapters (Thermo Fisher Scientific, USA) and the samples analysed using the Ion PGM Hi-Q Sequencing Kit using an Ion 314 Chip (Thermo Fisher Scientific, USA).