title: GSM6746025: Ube2i cKO GV oocyte 1 [6299a_SB17]; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq library was generated according to SMART-seq v4 Ultra low input RNA kit (Takara). In brief, isolated cells were lysed in lysis buffer, 3-SMART-seq CDS primer II and V4 oligonucleotide were added for first stranded cDNA synthesis.  cDNA was amplified using PCR Primer II A, and subsequently purified using Ampure XP beads (Beckman). Illumina library was prepared using Nextera XT DNA library preparation kit (Illumina) and sequenced using Illumina Novaseq.