title: GSM7328476: EHTs, AMR 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from EHTs 24 hours after treatment using the Nucleospin mini kit (Macherey-nagel, 740955.250) Libraries were generated from 200 ng RNA using the KAPA-RNA HyperPrep kit with RiboErase (Roche) to remove ribosomal RNA (ie, rRNA depleted total RNA), according to manufacturer's instructions. Library amplification was performed with 11 cycles. Libraries were multiplexed and sequenced on NextSeq500 Illumina to generate 38bp PE reads.