title: RNA-seq of Mus musculus: adult female lung that received sterile synthetic cystic fibrosis sputum medium (SCFM2)
design: Lungs were harvested and immediately homogenized in TRIzol, then stored at -80C until RNA extraction. RNA was extracted using the TRIzol protocol. RNA was fragmented with the NEBNext Magnesium fragmentation module per the manufacturer's instructions. Sequencing libraries were prepared with the NEBNext Small RNA Library prep kit. Ribosomal RNA was depleted using the QIAseq FastSelect Epidemiology kit, and adapter dimers were removed by size selection on a 5% TBE polyacrylamide gel.