title: GSM7329796: CTC 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from TSCs, PCs and CTCs was extracted and purified using RNeasy Plus Micro Kit (Qiagen, USA). Quality control of the extracted RNA was assessed by RNA integrity using Bioanalyzer 2100 system (Agilent, USA). Ribosomal RNA was depleted using Ribo-Zero Plus rRNA Depletion kit (Illumina, USA). DNA library was prepared by 3' adenylation of DNA fragments and ligation of the adapter sequences. Individual libraries were quantified by qPCR and all libraries were normalised to 2 nM. Libraries were pooled and clustered through the Illumina cBot system using TruSeq PE Cluster Kit v3 reagents