title: GSM7791297: WT Female; Mus musculus; RNA-Seq
library_construction_protocol: Brain dissociation and endothelial cell isolation was performed using the adult brain dissociation kit and CD31 magnetic beads from Miltenyi Biotec. Briefly, meninges were removed mechanically and brain cortex from 9-month old APP/PS1 transgenic and WT mice were collected into ice-cold PBS. After magnetic bead separation, the flow through containing endothelial cells was subjected to single-cell RNA sequencing (scRNA seq) using 10X Chromium. Single cell RNA libraries were prepared using 10X Genomics Chromium Single Cell 3' Reagent Kits following manufacturer's protocol.