title: GSM8421903: Non-wounded scRNA; Mus musculus; RNA-Seq
library_construction_protocol: Small pieces of tissue were incubated with 1 mL of 0.4 mg/mL Liberase TL (Roche, 05401020001) in DMEM with 100 units/mL of penicillin and 100 mg/mL of streptomycin, then washed with PBS and incubated with 0.05% TrypLE™ Select. The cell suspension was washed with 10% FBS in DMEM with 100 units/mL of penicillin and 100 mg/mL of streptomycin, then incubated with 100 units/mL of DNase I (New England BioLabs, M0303). The cell suspension was washed with 0.04% (w/v) BSA in PBS and filtered through a 40 µm strainer to prepare a single-cell suspension. To obtain a live single-cell suspension, Propidium Iodide (BioLegend, 421301) negative and Vybrant DyeCycle Violet (Invitrogen, V35003) positive single cells were sorted. Library construction was performed using the 10x Chromium Single Cell v3 platform. Sequencing was conducted on the NovaSeq 6000 with v1.5 reagents. Basecalling and sample demultiplexing were carried out using Illumina's BaseSpace software