title: GSM7151666: Vehicle control Female; Rattus norvegicus; RNA-Seq
library_construction_protocol: Neonatal rat frontal cortex tissues were quickly removed in cold buffer and collected in RNAlater until use. RNA was extracted using mirVana miRNA Isolation Kit. RNA libraries were prepared for sequencing using standard Illumina protocols. To isolate mRNA, total RNA was treated with DNase I, and Oligo(dT) was used. The mRNA was then mixed with a fragmentation buffer, resulting in the fragmentation of the mRNA. cDNA was synthesized using the mRNA fragments as templates, and the resulting short fragments were purified and resolved with an EB buffer for end reparation and the addition of a single nucleotide A (adenine). The short fragments were then connected with adapters, and suitable fragments were selected for PCR amplification. During the quality control (QC) steps, the sample library was quantified and qualified using the Agilent 2100 Bioanalyzer and ABI StepOnePlus Real-Time PCR System.