title: GSM8152046: apocrine gland anal sac adenocarinoma (AGASAC), 1  [a1.1 RNA-seq]; Canis lupus familiaris; RNA-Seq
library_construction_protocol: Total RNA was extracted utilising the PureLinkTM FFPE Total RNA Isolation Kit (Thermo Fisher Scientific Inc., Waltham, MA, USA). After DNase I treatment 500 - 1000 ng total RNA was used for library construction. QuantSeq 3' mRNA-Seq FWD Library Preparation Kit (Lexogen GmbH, Vienna, Austria) was used according to the manufacturer's guidelines (user guide 015UG009V0251) using the low-quality RNA protocol.