title: GSM8249402: iCD4+Treg-like day14 by AMRT; Homo sapiens; RNA-Seq
library_construction_protocol: Naive CD4+T cells (CD4+CD8-CD25-CD45RA+CCR7+) and CD25+CD127-Treg (CD4+CD8a-CD25highCD127low) were sorted by FACS using CD4+T cells which werer negatively isolated by MACS from purchased frozen PBMCs. iPSC-derived CD4+T cells were differeniated from iPSC-derived hematopoietic progenitor cells by ATO method, and 8 weeks after ATO culture CD4+CD8-CD25- cells were sorted by FACS. The cells were cultured for 7 or 14 days with anti-CD3/28 antibody stimulation in the presence of AMT or AMRT, and library for scRNA-seq was prepared from each sample using Chromium Next GEM Single Cell 3ʹReagent Kits v3.1 (10X Genomics) according to manufacturer instructions. Library was performed according to manufactur's instruction (10xChromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index))