title: WES of H3122-CR1 cells
design: DNA purity, quantity, and integrity were determined with Qubit (ThermoFisher Scientific) and TapeStation 4200 (Agilent) analysis prior to Whole Exome Sequencing (WES) library preparation. The SureSelect Human All Exon V8 exome probes (Agilent) and SureSelectXT Reagent Kits (Agilent) were used with an input of 200 ng of gDNA to generate WES libraries. Paired-end sequencing reads of 150 bp were generated on NovaSeq 6000 (Illumina) sequencer at a target depth 100X coverage per sample. Raw sequencing reads were de-multiplexed using bcl2fastq. Variants and associated biological significance were analyzed with the nf-core/sarek pipeline (version 2.71), using default annotations for the human genome assembly GRCh38 and Mutect2, Strelka, and FreeBayes tools.