title: GSM6744482: Mel167-M-B2; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Single cell were either manully picked or sorted into the scBS lysis buffer by FACS, and DNA was released after proteinase treatment at 50C and then bisulfite converted. After bislfite purification, DNA was complemented with the biotinated random primer (5'-biotin-CTACACGACGCTCTTCCGATCTNNNNNNNNN-3') and 50 units of klenow polymerase (3' to 5'exo-, New England Biolabs). This random priming was repeated 5 times in total. The second strands were synthesized using another random primer (5'-AGACGTGTGCTCTTCCGATCTNNNNNNNNN-3') and the final libraries were generated after 8-12 cycles PCR amplification with the Illumina universal PCR primer and Illumina indexed primer.