title: GSM6652444: mural granulosa cells, Control 1, 2h; Mus musculus; RNA-Seq
library_construction_protocol: A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations.RNA degradation and contamination was detected by 1% agarose gels; RNA purity was checked using the Qubit® 3.0 Flurometer（Life Technologies,   CA, USA）; RNA integrity and concentration was assessed using the RNA Nano 6000 Assay   Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (#E7530L, NEB, USA) following the manufacturer's recommendations and index codes were added to attribute sequences to each sample.