library_construction_protocol: Blood was obtained from healthy donor leukopaks (New York Blood Center, New York, USA) and peripheral blood mononuclear cells (PBMCs) were isolated using ACCUPSIN Tubes according to the manufacturer's protocol (Sigma-Aldrich, Missouri, USA). Cell viability was assessed with Trypan Blue Stain (BioRad, California, USA). Cells were cultured in RPMI 1640 Medium (Gibco, ThermoFisher Scientific, Massachusetts, USA) with 10% Fetal Bovine Serum (Corning, Arizona, USA) and 1x Penicillin-Streptomycin (Gibco). Single-cell RNA sequencing (scRNA-seq) was performed using the 10x Genomics platform (California, USA). Chromium Next GEM Single Cell 3ʹ Kit v3.1 was used for sample processing and library construction. The cells were loaded into the Chromium Controller with a target of 10,000 cells collected per sample and were captured and processed according to the manufacturer's instructions. Library QC was performed using the Agilent Bioanalzyer and High Sensitivity DNA kit (California, USA) and DNA concentrations were measured using a Qubit fluorometer (ThermoFisher Scientific). Sequencing was performed using the Illumina NextSeq 2000 (California, USA). Libraries were loaded at a concentration of 650 pM and were sequenced using a P3 200 cycle sequencing kit.