library_construction_protocol: After perfusion of the mice with PBS, lung was prepared and kept in cold DMEM/F12 media. Three lungs of each group was collected together and dissociated as single cell by 37C incubation for 30min in dissociation buffer(papapin and liberase DH in DMEM/F12 media). After the dissociation, cells were counted using Luna-II automated cell counter (Logos Biosystems) and captured using the BD Rhapsody system. BD Rhapsody Express system (BD) was used for the single cell capture and mRNA harvest. Single cell whole transcriptome libraries were prepared according to the manufacturer's instructions using BD Rhapsody WTA Reagent kit (BD, 633802) and sequenced on the Illumina NextSeq 500 using High Output Kit v2.5 (150 cycles, Illumina) aiming sequencing depth of >20,000 reads per cell for each sample.