library_construction_protocol: Cells were treated with Lysis buffer and reagents following the instruction of TruePrep DNA Library Prep Kit V2 for Illumina. ATAC-seq libraries were prepared using the TruePrep DNA Library Prep Kit V2 for Illumina from Vazyme biotech co.. Fifty thousand cells were used as starting material for samples. Library were amplified using appropriate cycles on the thermocycler. Post amplification libraries were size selected using VAHTS DNA Clean Beads from Vazyme biotech co.. Libraries were validated using Qubit, AATI and QPCR.