library_construction_protocol: The DNA was extracted and the samples were bisulfite converted by using EZ DNA Methylation-Lightning ™ Kit according to manufacturer's instructions. The samples were pooled, purified using DNA Clean & Concentration ™ Kit. Targeted bisulfite Seq; DNA methylation was assessed by EZ DNA Methylation-Lighning ™ Kit (Zymo Research, D5030), according to the manufacturer's instruction.