library_construction_protocol: The Visium spatial gene expression protocol was followed according to the manufacturer's instructions (#PN-1000187, 10X Genomics). Briefly, tissue permeabilization was followed by cDNA synthesis, second strand synthesis and cDNA denaturation. The ideal cycle number was determined by qPCR and cDNA was amplified and cleaned using SPRIselect. The amount of cDNA was measured to determine the cDNA input for library construction. Library construction was performed following the Visium protocol. Briefly, cDNA was fragmented and submitted to end repair and A-tailing. Adaptor sequences were ligated, and the post-ligation product was cleaned and subjected to Index PCR. Overall, the P5, P7, i7 and i5 sample indexes, and TruSeq Read 2 were added.