library_construction_protocol: Total RNA was harvested using a RNA quick purification kit (Esunbio). 1.3μg of total RNA was used for the construction of the sequencing libraries. The mRNA was enriched by oligodT according to instructions of NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB,USA).And then fragmented to approximately 200bp. Subsequently, theRNA fragmentswere subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycleaccording to instructions of NEBNext® Ultra™ RNA Library Prep Kit for Illumina.