library_construction_protocol: RNA was extracted using Trizol reagent following manufacturer's instructions. In brief, Trizol samples were thawed and RNA was extracted by phenol-chloroform separation. RNA was precipitated using Glycoblue reagent as a co-precipitant following manufacturer's recommendations. Following phenol-chloroform extraction, RNA samples were then processed using the Zymo RNA Clean and Cocentrator kit with DNase treatment following manufacturer's instructions. RNA yield was measured using Qubit High-sensitivity RNA assay kit following manufacturer's instructions. 2x100bp libraries were made using 100ng total RNA using an Illumina TruSeq stranded mRNA kit.