library_construction_protocol: Conditional senescence Eµ-myc;Suv39h1-;Bcl2;Suv39h1-ER; lymphoma model is generated as in previous publication (Milanovic el al., Nature 2018). Lymphoma cells were treated into TIS (1 uM 4-OHT+ 0.05 ug/ml ADR) or non-TIS (0.05 ug/ml ADR) and collected on day5 post-treatment. Dead-cell removal was performed according to manufacturer's protocol and proceeded with scRNAseq procedures. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, GCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and the apropiated sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.