library_construction_protocol: 3g seedlings for each set were fixed under vacuum for 20 min in 1xPBS  (10 mM PO43â, 137 mM NaCl, and 2.7 mM KCl) containing 1% Formaldehyde (F8775Â SIGMA). The reaction was quenched by adding glycine to a final concentration of 62 mM. Chromatin immunoprecipitation (ChIP) was performed as described (12), with the exception thatÂ 100 Âµl FLAG M2 agarose affinity gel antibody was used (A2220 SIGMA-Aldrich) per sample. Sequencing libraries were prepared usingÂ TruSeq ChIP Sample Preparation Kit (IlluminaÂ  IP-202-1024) and samples sequenced on NextSeq500 machine from Illumina using NextSeqÂ® 500/550 High Output Kit v2 (75 cycles) TG-160-2005. Sequence reads were analysed using in-house pipelines. The efficiency of ChIP materials was assessed by qPCR prior to in-house library preparation. Sequencing libraries were prepared usingÂ TruSeq ChIP Sample Preparation Kit (IlluminaÂ  IP-202-1024) and samples sequenced on NextSeq500 machine from Illumina using NextSeqÂ® 500/550 High Output Kit v2 (75 cycles) TG-160-2005. Sequence reads were analysed using in-house pipelines. The libraries were sequenced at the Sainsbury Laboratory Cambridge University Services sequencing facility.