library_construction_protocol: Total RNA from soybean seeds was extracted using the Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer's protocol with some modification as described previously (Farrell et al., 2017). The mRNA stranded library prep kit from KAPA Biosystems and 750 ng of total RNA were used to build each library following manufacturers recommended protocol with nine cycles of PCR.