library_construction_protocol: RNA was extracted from 200,000 frozen cell pellets using the RNAeasy Mini Kit (Qiagen #74104) following the manufacturer's instructions. otal RNA's integrity and concentration was assessed using RNA ScreenTape Analysis (Agilent #5067-5576) on an Agilent 2200 Tapestation Samples were sequenced to reach a minimum number of 30M unique valid paired reads.