library_construction_protocol: FACS isolated cells were loaded into 4 different lanes on a Chromium Next GEM Chip. Lane 1 was loaded with IL-1R KO cells from Mtb-infected lungs. Lane 2 was loaded with IL-1R KO cells from naive lungs. Lane 3 was loaded with wild-type B6 cells from Mtb-infected lungs. Lane 4 was loaded with wild-type B6 cells from naive lungs. All 4 lanes of the Chromium Next GEM Chip were loaded with 12500 cells with a target of 7000 single cells per lane. The scRNA-sequencing libraries were generated using the v3.1 chemistry Chromium Single Cell 3' Reagent Kit (10X Genomics) following this kits protocol. Following cDNA purification, samples were decontaminated by 2 rounds of centrifugation through 0.2 µM filter microcentrifuge tubes and then removed from the BSL3. Library preparations were completed outside of the BSL3 following the 10X Genomics protocol for the cDNA. Quality control of the libraries was performed with a Fragment Analyzer (Agilent). Libraries were sequenced on a NovaSeq 6000 (Illumina) using one lane of a S1 flow cell and the following cycles read 1 (28 cycles), i7 index (10 cycles), i5 index (10 cycles), read 2 (90 cycles).