library_construction_protocol: The cells were harvested in the exponential growth phase and frozen in liquid nitrogen. Total RNA was isolated from 3 biological replicates of C. glutamicum cells by a Quick-RNA Miniprep Plus kit (Zymo Research). The samples were treated with DNase (Roche Diagnostics) and RNA was purified with an RNA Clean&Concentrator-5 kit (Zymo Research).  cDNA library enriched for primary 5′-transcripts according to Peifer-Sancar et al. (2013): I) Depletion of stable RNAs using the Ribo-Zero rRNA removal kit II) RNA fragmentation by metal hydrolysis III)  Digestion of non native 5'-Mono/Di-phosphate ends by Terminator 5′-phosphate-dependent exonuclease IV)Conversion of 5′-triphosphates of primary transcript ends to monophosphates to enable adapter ligation V)Full RNA adapter ligation VI)cDNA synthesis by the usage of a loop adapter which hybridizes via a 3′-NNNNNNN-tail to the 3′-end of the RNA fragment VI)Amplification and Indexing by Adapter-specific primer