library_construction_protocol: Total RNA was extracted from frozen ground tissue using the Direct-zol™ RNA MiniPrep Plus (Zymo Research) which includes DNAse treatment. Yield and purity were measured with a NanoDrop 2000 spectrophotometer (Thermo Scientific) and RNA integrity (RIN) was measured on an Agilent 2200 Bio TapeStation system (Agilent Technologies) using Agilent High Sensitivity RNA ScreenTape. RNASeq library preparation was performed with the TruSeq stranded mRNA library prep kit (Illumina) preparing for 150 nucleotide paired-end sequencing. The 72 libraries were run on an Illumina HiSeq 4000 platform on a total of 16 lanes (2 flow cells) with seven libraries per lane, yielding 5.2 billion paired-end reads.