library_construction_protocol: RNA libraries of four biological lines of AT2 organoids at early (P1,4,6,7) and late (P12,13,16,17) passage were generated using an RNeasy kit (Qiagen, 74004) including the optional DNAse digestion step. The quality of the RNA libraries was validated on Agilent 2200 Tapestation before sequencing on an Illumina NovaSeq 6000 at Novogene (novogene.com). RNA libraries were prepared for sequencing using standard Illumina protocols