library_construction_protocol: The samples were then subjected to ultracentrifugation (38,000 rpm, 3 hours, 4℃), and the ribosome-protected RNA fragments were isolated using the RNA Clean and Concentrator-25 kit (Zymo Research, USA). Subsequent steps involved rRNA removal and construction of cDNA libraries using the Next Multiple Small RNA library preparation Set for Illumina (New England Biolabs, USA) as described previously, The constructed cDNA libraries were sequenced using the Illumina HiSeqTM ×10 platform