library_construction_protocol: Single cell suspensions were obtained from iLNs of host mice (Tcra-/-), and fate-mapped (ZsGreen+) WT or KO cells were FACS-sorted. RNA was purified using the RNeasy Plus Micro Kit (Qiagen). Library construction was performed at Duke Sequencing and Genomic Technologies Shared Resource facility using the Clontech SMARTer v3/v4 Ultralow Input RNA-seq Kit (Takara Biosciences).