library_construction_protocol: Blood was collected from healthy volunteers and collected into either NaCit or EDTA collection tubes. RBCs were lysed for 20 mins in 1x ACK Buffer. Cells were washed in PBS twice by centrifugation at room temperature for 5 min at 350g. Fixation was performed according to each scRNA-Seq kit  manufacturer specific protocol. The number of cells fixed is indicated experimental metadata. Honeycomb HIVE libraries were processed with manufacture's protocol cat 10347 v.0.3 revA. 10x Genomics Flex batches 1 and 2 were performed with Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples CG000527, Rev B. Batches 3 and 4 were performed with, Rev D. There are no material differences in reagents or workflow with Rev B being a more detailed protocol than B.