library_construction_protocol: The genomic DNA samples were extract from peripheral blood by using phenol-chloroform DNA isolation method. The exome sequences were efficiently enriched from 0.4 μg genomic DNA using Agilent SureSelect Human All Exon V6 (Agilent, USA, Catalog #: 5190-8864) according tothe manufacturer's protocol. Firstly, genomic DNA was randomly fragmented to an average size of 180-280bp by Covaris S220 (Covaris, USA). Remaining overhangs were converted into blunt ends via exonuclease polymerase activities. Secondly, DNA fragments were end repaired and phosphorylated, followed by A-tailing and ligation at the 3' ends with paired-end adaptors. DNA fragments with ligated adapter molecules on both ends were selectively enriched in a PCR reaction. After PCR reaction, libraries hybridize with liquid phase with biotin labeled probe, then use magnetic beads with streptomycin to capture the exons of genes. Captured libraries were enriched in a PCR reaction to add index tags to prepare for sequencing. Products were purified using AMPure XP system (Beverly, USA), libraries were analyzed for size distribution by Agilent 5400 system (Agilent, USA )and quantified by QPCR (1.5 nM). The Qualified libraries were pooled and sequenced on Illumina platforms with PE150 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing, China), according to effective library concentration and data amount required.