library_construction_protocol: RNA was harvested using the Rneasy mini plus kit by Qiagen. A total of 1.3 micrograms (μg) of RNA was utilized for the construction of sequencing libraries, which is a process for preparing RNA samples for next-generation sequencing. The libraries for RNA-seq were prepared using the SMARTer mRNA-Seq Library Prep Kit, adhering strictly to the protocols provided by the manufacturer. This step involves converting RNA into a library of cDNA fragments with adaptors attached to each end, a prerequisite for sequencing.