library_construction_protocol: RNA was harvested from FACS-sorted populations using RNeasy kits (Qiagen). 20 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A) mRNA magnetic isolation module and AMPure XP SPRIselect beads (Beckman Coulter),