library_construction_protocol: Tumor was mechanically and enzymatically dissociated using a human tumor dissociation kit, according to the manufacturer's instructions (130-095-929, Miltenyi Biotec). In brief, tumor was cut into small pieces and transferred into gentleMACS C tubes containing the enzyme mix. The dissociation was performed using the gentleMACS Octo Dissociator with heaters and with the human tumor dissociation 37C_h_TDK_1 program. Samples were homogenized before being applied to a MACS SmartStrainer 70µM (130-110-916, Miltenyi Biotec) placed on a 50 mL tube. Filters were washed with 20 mL serum-free RPMI (L0500-500, Dutscher) and then centrifuged at 300g for 7 minutes. After complete aspiration of the supernatant, tumor cell suspensions were resuspended in RPMI and counted with trypan blue to remove dead cells. Cells were frozen in a solution of 50% FBS (Dutscher), 40% RPMI and 10% DMSO (P60-36720100, Dutscher) until further use. PBMC were isolated and frozen as described above. On the day of the single cell experiment, the samples first underwent a specific preparation protocol. Briefly, the samples were thawed following the 10X genomics thawing protocol based on cascade dilutions. Dead cells were then removed with the Dead Cell Removal kit according the manufacturer's instructions (130-090-101, Miltenyi Biotec). CD3 T cells were then isolated for each sample on a magnetic column after labelling with CD3 MicroBeads (130-050-101, Miltenyi Biotec). To better purify CD3 T cells, we labelled the samples with an anti-TCRαβ-PE (Clone IP26A, Beckman Coulter) and an anti-TCRγδ (Clone IMMU510, Beckman Coulter) in order to sort the positive cells for one of these two markers with an ARIA Fusion sorter. Finally, we resuspended the cells at 1000 cells/µL before proceeding with the cell encapsulation step according to the manufacturer's instructions via the Chromium device (10X genomics). Library preparation was performed using library prep for 5'mRNA and VDJ (10X Genomics)