library_construction_protocol: RNA-Seq libraries were generated using 40 ng and 80 ng of total RNA from cancer cells and THP-1, M0-macrophages, M1/M2 macrophages,FPCO-macrophage respectively RNA-seq library was prepared using Ion Ampliseq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific, Carlsbad, CA), according to the manufacturer's protocol.