library_construction_protocol: Animals were lysed by grinding in a mortar and pestle cooled in liquid nitrogen in the presence of frozen PLB (20mM Tris pH8.0, 140mM KCl, 1.5mM MgCl2, 1% Triton). RNA was extracted by trizol. RNA extracted, rRNA depleted by C. elegans-specific hybridization oligos, libraries  prepared using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina sequencing