library_construction_protocol: After a 24 h incubation, fibroblasts were lysed, and RNA isolation was performed with RNeasy Mini kit (Qiagen, Hilden, Germany) as recommended by the manufacturer. The RNA concentration and purity were measured by NanoDrop (ND-100, RNA-40). NEB NEXT ULTRA II Directional RNA Library Preparation Kit (Illumina,San Diego, CA, USA)