library_construction_protocol: Single-cell suspension was processed and loaded into a Chromium Next GEM Chip G (10x Genomics) to generate single-cell gel bead in emulsions on a 10x Genomics Chromium Controller. Single-cell RNA-seq libraries were constructed using a Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10x Genomics).