library_construction_protocol: RNA was harvested with Trizol reagent, followed by chloroform extraction and isopropanol precipitation. Genomic DNA was depleted using Turbo DNAse, followed by cleanup with RNAclean beads. Tether-seq libraries were generated following standard protocols using the SMARTer Stranded Total RNA-Seq Kit-Pico Input Mammalian, V2 (Takara Bio USA, cat. 634413).