library_construction_protocol: Cells were isolated using the Miltenyi Adult Brain Dissociation Kit with minor modifications: enzymatic dissociation was done using the Miltenyi AutoMacs dissociator at 4 °C for 1 h, using alternating “stirring” and “grinding” repeating a total of 12 times for 1 h. Live cells were sorted on a Sony SH800 Cell Sorter using Sytox blue (Thermo Fisher) for removing necrotic cells and Annexin-V (Cell Signaling Technologies) for removing apoptotic cells according to the manufacturer's instructions. Live cells (33.8 uL) were input directly to the Chromium Controller (10x Genomics). The cell isolation method was chosen from a comparison study of cell isolation methods including Percoll gradient, various enzymatic digestion methods, and red blood cell lysis buffers for least effect on transcriptome and most brain cells of various types isolated. Libraries were prepared using the Chromium Single Cell 3'Reagents Kit, V2 chemistry, according to the manufacturer's protocol (10x Genomics). Generated libraries were pooled and sequenced targeting at least 30k reads per cell on Illumina NextSeq.