library_construction_protocol: RNA was extracted using QIAGEN RNeasy Kits according to the manufacturer's instructions. 3'-Digital Gene Expression RNAseq Library Kit (3'-DGE) Messenger RNA was extracted using NEBNext Poly(A) mRNA Magenetic Isolation Module according to the manufacturer's protocol, and 3' RNA-Seq library were prepared using the 3'-Digital Gene Expression RNAseq Library Kit (Amaryllis Nucleics). Libraries were sequenced on an Illumina NextSeq 500 machine (10-25M reads, single-end, 80 bp).