library_construction_protocol: Total RNA was extracted using the Trizol Reagent (Invitrogen, Carlsbad, USA) from a pool of 30 mosquitoes treated with or without Bs. A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using Illumina TruseqTM RNA sample prep Kit following manufacturer's recommendations and index codes were added to attribute sequences to each sample.