library_construction_protocol: Cell pellets were harvested by centrifugation at 4,000 × g and flash frozen in liquid N2. Total RNA was extracted using the MasterPure™ Complete DNA and RNA Purification Kit from Epicentre. All samples were treated with Invitrogen TURBO DNA-Free kit to remove DNA contamination. The Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus (Illumina) was used for rRNA depletion and library preparation. The Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus (Illumina) was used for rRNA depletion and library preparation.