library_construction_protocol: RNA was extracted from crushed tumour pieces using NucleoSpin® RNA plus kit (MachereyNagel, 40955) per manufacturers protocol. Libraries were prepared using QuantSeq 3' mRNA-Library Prep Kit FWD (Lexogen). RNA quantity and quality were assessed on TapeStation 4150 (Agilent). Reads were sequenced on NextSeq500 (Illumina) using 75-cycle High Output Reagent kits