library_construction_protocol: Cells were wahed and harvested by wash buffer and incubated with activated beads. Then primary antibody was added, followed by Guinea Pig anti-Rabbit IgG antibody. After incubation, pA-Tn5 adapter complex was added. The purified DNA was amplified, then 200-1,000bp fragments were enriched for sequencing. CUT&Tag libraries were prepared by Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyem, TD903-01 )