library_construction_protocol: 25 mg of mixed kidney tissues were fixed with 1.5% formaldehyde for 20 minutes. After quenching and cell lysis, the chromatin was digested with micrococcal nuclease and sonicated with ultrasonicator ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit. 10ng of DNA was used as starting material for input and ip samples. Post amplification libraries were sized selected at 100-300bp in length using Agencourt AMPure XP beads.