library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 150ng of total RNA was used for the construction of sequencing libraries. 150ng of total RNA was used to enrich for poly-adenylated RNA, which were heat-fragmented to a target size of 180bp and converted to cDNA using random primers with Superscript II (Invitrogen), then used for library prep using the Illumina TruSeqRNA library kit.