library_construction_protocol: Cryopreservation. Snap freezing. Samples stored at -80 oC and processed in parallel for each sequencing round Cortical organoids from human pluripotent stem cells differentiated as described in (doi: 10.1038/nmeth.3415.)  Follow the standard protocol for RNeasy Plus Mini Kit (50) Qiagen TruSeqÂ® Stranded mRNA Library Prep (48 Samples) (Ribozero)