library_construction_protocol: EVs were isolated from SP groups (3 biological replicates/group; 2.5 mL SP/replicate) using Ultracentrifugation and PURE-EV® (HansaBioMed Life Sciences) size exclusion chromatography (SEC). Total RNA was isolated from EV samples using Trizol and miRNeasy Micro Kit. Small-RNA libraries were prepared using QIAseq miRNA Library Kit