library_construction_protocol: RNA from sorted LSECs was extracted using TRIzol™ LS Reagent (ThermoScientific, #10296028) according to the manufacturer's instructions with an additional step of RNA precipitation with glycogen (ThermoScientific, #R0551). Library was prepared using Ion AmpliSeq™ Transcriptome Mouse Gene Expression Kit according to the manufacturer's protocol (Thermo Fisher Scientific)