library_construction_protocol: Roots cultured on 0 and 300 mg/l PS-COOHs concentrations for 9 days are used to extract RNA.The total RNA was extracted using TRIzol reagent (Invitrogen, California, USA). Then, the RNAs' purity and quantity were checked using an RNA Nano 6000 Assay Kit for the Bioanalyzer 2100 system (Agilent Technologies, California, USA). Afterwards, approximately 10 µg of total RNA was used for RNA-seq library  construction. RNA libraries were prepared for sequencing using standard Illumina protocols. And 150-bp paired-end reads were produced.