library_construction_protocol: Cryopreserved initial passage organoid cell stocks were thawed in a 37 °C water bath for 1 min and transferred to DMEM/F-12 (Gibco) followed by centrifugation at 500× g for 5 min at 4℃. Cells were further washed twice with cold DPBS (Gibco) containing 0.04% BSA (Miltenyi Biotec) and filtered through 40 µm cell strainers (Flowmi). Cell suspensions from two different donors were pooled to equal cell concentrations. Pooled cell suspensions were then loaded targeting recovery of 10,000 cells per channel to construct single-cell RNA sequencing (scRNA-seq) libraries using Chromium Next GEM Single Cell 5' v2 kit (10x Genomics) according to the manufacturer's instructions. Generated libraries were sequenced on a Illumina NovaSeq 6000 at a paired-end, 150 bp reads configuration aiming 400 million reads per library.