library_construction_protocol: Total RNA was extracted using a Trizol kit A fragmentation buffer was added to randomly fragment the mRNA, and then use mRNA as a template to reverse-transcribe into cDNA. Double-stranded cDNA was purified using AMPure XP beads, end-repaired, and ligated to Illumina sequencing adapters to obtain the final cDNA library The cDNA library was sequenced using Illumina Novaseq6000.