library_construction_protocol: Leaves were sampled at 6, 12, 24, 36, and 48 hours post inoculation. Experiment was performed in controlled environment (a greenhouse), in temperature regime 21 C day / 19 C night and under 14 h photoperiod. Seeds were scarified before sowing to brake seed dormancy resulting from hard seed coat and provide even germination. Pots (11 cm  11 cm  21 cm) with sterilized soil were used. The inoculation was done using Colletotrichum lupini strain Col-08, obtained in 1999 from the stem of narrow-leafed lupin plants cultivated on field in Wierzenica (522742N 170405E) in Great Poland region. The isolate was cultured on SNA medium for 21 days at 20C under black light to induce sporulation. The inoculation was performed 4 weeks after sowing, when the plants reached 4-6 leaf stage, by spraying of conidial spore suspension  in concentration 0,5 x 106 conidia per ml. After inoculation, plants were kept for 24 hours in darkness under ~100% humidity and temperature of 25C to facilitate conidia germination and infection process. Afterwards, plants were grown under 14-h photoperiod in temperature regime 22C day / 19C night and humidity ~70%. Frozen leaf tissue (50 mg) was homogenized in 2 ml tubes (Eppendorf, Hamburg, Germany) and two stainless steel beads ( 5 mm) using TissueLyser II (Qiagen). SV Total RNA Isolation System (Promega, Madison, WI, USA) was used for RNA isolation without any changes to the protocol except extending DNAse I digestion to 25 min. RNA purity and integrity of the samples prepared for sequencing were measured using an Experion Automated Electrophoresis System (Bio-Rad, Hercules, CA, USA). TruSeq RNA Sample Preparation v2 Guide, Part # 15026495 Rev. F