library_construction_protocol: RNA was extracted and purified from each sample in each group using Trizol, and agarose gel electrophoresis was utilized to analyze the integrity of RNA and the presence of DNA contamination. RNA was isolated using TRIzol reagent, and RNA integrity was evaluated using the Agilent 2100 Bioanalyze. RNA was isolated using TRIzol reagent and  NEBNext® Ultra™ RNA Library Prep Kit for Illumina® was  used for the construction of sequencing libraries. RNA-seq( long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs ))