library_construction_protocol: The tumors were digested both mechanically and enzymatically (Collagenase/Hyaluronidase, and DNaseI) and single cell suspensions of viable cells were prepared. mRNA from each individual cell was captured and tagged with barcodes using 10X Genomics microfluidic platform. Barcoded cDNA library was prepared as per manufacturer's instructions, 10X Genomics.