library_construction_protocol: Mice were euthanized and brown adipose tissue was excised and cut in half, with one half flash-frozen in liquid nitrogen. Flash-frozen brown adipose tissue were later pulverized and DNA was extracted using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen). DNA quality, quantity, and purity (260/280 absorbance ratios) were assessed using a Fragment Analyzer, a Qubit fluorometer, and a Nanoquant plate using an Infinite 200 PRO absorbance reader, respectively. DNA was diluted to a normalized concentration (0.222 ng/uL) and sequenced.