library_construction_protocol: The cell suspension was loaded into Chromium microfluidic chips with 3' chemistry and barcoded with a 10× Chromium Controller (10X  Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and  sequencing libraries constructed with reagents from a Chromium Single Cell 3' v2 reagent kit (10X Genomics) according to the manufacturer's  instructions.