library_construction_protocol: Cells were isolated with LCM in 6.4 μL of cell lysis buffer (0.6 μL of 5× SuperScript II buffer, 0.1 μL of 100 μM Oligo dT VN, 0.8 μL of dNTP mix [25 mM each], 0.25 μL of 40 U/μL recombinant RNase inhibitor [Takara Bio], 0.5 μL of 100 mM DTT, 0.06 μL of 1 M MgCl2 [Sigma-Aldrich], 2 μL of 5 M Betaine [Sigma-Aldrich], 0.16 μL of 1:500,000 diluted ERCC RNA Spike-In Mix 1, 0.4 μL of 5% SDc, and 1.53 μL of DDW) in the caps of PCR tubes, and spun down into the tubes by brief centrifugation. The isolated cells were lysed at 72°C for 6 min, followed by addition of 2.8 μL quenching buffer (0.7 μL of Triton X-100, 0.7 μL of 2% BSA, and 1.4 μL of 5× SuperScript II buffer [Thermo Fisher]). 1.6 μL of a template-switching mixture (0.1 μL of 100 μM N-template-switching oligo, 0.25 μL of SuperScript II, 0.25 μL of SuperScript III, 0.2 μL of recombinant RNase inhibitor, and 0.8 μL of DDW) was added to cell lysate in the lysis buffer of smart-seq2, followed by the cycling RT program for DRaqL-Smart-seq2. Then, cDNA amplification was performed by adding 15 μL of Seq amp PCR mixture (12.5 μL of 2x SeqAmp buffer (Takara Bio), 0.05 μL of 100 μM N-IS PCR primer, 0.5 μL of SeqAmp DNA polymerase (Takara Bio), and 1.95 μL of DDW) and applying the SeqAmp thermal cycling program. For oocytes, we used a PCR program of 18 cycles. The amplified cDNA (25.8 μL) was purified with a 0.8× volume of AxyPrep MAG PCR clean-up reagent according to the manufacturer's instructions. Libraries of the cDNAs amplified with the Smart-seq2-based methods were prepared using the library construction module of the SMART-Seq v4 3' DE Kit, according to the manufacturer's instructions. Briefly, 10 to 12 purified cDNA samples were pooled and purified with a 0.8× volume of Axyprep reagent. 300 pg to 400 pg cDNAs were tagmented using the Amplicon Tagment Mix (ATM) of the Illumina Nextera XT DNA Sample Preparation Kit (Illumina). 3'-ends of the tagmented cDNAs were amplified with Nextera PCR Master Mix and dual index primers (Reverse PCR primer HT Index and Tn Forward PCR Primer HT Index in the SMART-Seq v4 3' DE Kit) designed for TnRP1 and TnRP2 sequences. All libraries were mixed and sequenced using the NextSeq 500/550 High Output Kit v2.5 (75 Cycles) with paired-end mode using Read2 (8 bps) for the in-line index and with dual-index mode (8 bps).