library_construction_protocol: 12-day-old seedlings of AGO1 truncation mutants in the ago1-36 background were ground to a fine powder in liquid nitrogen and subjected to RNA extraction using TRI Reagent, polyadenylated RNA was isolated from total RNA using the Magnetic mRNA Isolation Kit (NEB). RNA-seq libraries were constructed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB7420) and sequenced on an Illumina Novaseq  platform to generate high-quality paired-end reads of 150bp in length.