library_construction_protocol: RNA extraction was performed using Trizol (Thermo Fisher, 15596026)-chloroform, followed by cleanup and elution into RNAse-free water using the RNeasy Minelute Cleanup kit (Qiagen, 74204) per manufacturer instructions. mRNA libraries were prepared using the Illumina Truseq RNA Library Prep Kit V2 (#RS-122-2001) per manufacturer instructions on 250 ng total RNA.