library_construction_protocol: The RNA immunoprecipitation assay was performed using the Magna RIP Kit (No.17-701, Millipore, MA, USA) following manufacturer's instructions. Using Qubit and Agilent2200 to detect antibody-specific enriched RNA fragments.Libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.