library_construction_protocol: For Sample 12-15, total RNA was extracted using Direct-zol RNA Miniprep Kit (Zymo, R2051) For Sample 12-15, Use NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) and NEBNext Ultra II Directional RNA Library Prep Kit NEB #E7760) for library preparation. For Sample 16, after tagmentation reactions with TnD transposase, libraries that passed QC were then pooled and sequenced.