library_construction_protocol: RNA was extracted using Monarch Total RNA Miniprep Kit (NEB) according to manfacuters protocol. RNA concentration and purity was assessed using a NanoDrop (ThermoFisher). RNA was stored at -80C until submission for NGS. Libraries were constructed using the Illumina Stranded mRNA Prep following the manufactures protocol. This is a poly-A enrichment approach. Unique dual-indexes were used to barcode libraries. RNA-Seq; Sequences were generated on a NovaSeq 6000 using a paired end, 100 base read length run. Files were provided in FASTQ format.