library_construction_protocol: We extracted RNA from 2x105 sorted CD4+ Foxp3- or CD4+ Foxp3+ populations in CD11c-  Cd70tg;CD27+/- Foxp3 reporter mice and CD27+/- mice (in duplicates), and RNA from 2x105  sorted CD4+ CD25+ in hCD2-Eomestg mice and wild-type C57Bl/6 mice (in triplicate) using  RNeasy Plus Mini kit according to manufacturer'sinstructions (Qiagen) Libraries were prepared using Ovation SoLo RNA-Seq System (NuGEN Technologies) and  underwent paired-end sequencing