library_construction_protocol: RNA was extracted from whole cells using a Trizol-based protocol, genomic DNA was digested using TURBO DNaseI according to manufacters protocol and samples were cleaned using Qiagen RNeasy kit. 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA samples were submitted to GENEWIZ Inc (South Plainfield, NJ). After that, rRNA was eliminated and performed cDNA library construction following GENEWIZ's protocols.