library_construction_protocol: RNA was extracted from subcellular fractions with Trizol LS according to the manufacturer's protocol. 2.5ug purified RNA was subjected to TimeLapse chemistry (Schofield et al., 2018) and then 1ug was used for library preparation with the SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara) according to the manufacturer's protocol.