library_construction_protocol: Briefly, ~ 107 intracellular tachyzoites were collected and the parasite nuclei were isolated by resuspension of the washed parasite pellet in NE Buffer. The nuclei were then processed according to the manufacturer's protocol and libraries were constructed using 'TD903 Hyperactive Universal CUT&Tag Assay Kit for Illumina' and 'TD202 TruePrep Index Kit V2 for Illumina' from Vazyme Biotech (#TD202, China) Libraries were sequenced on Illumina Novaseq platform at Novogene Science and Technology Co., Ltd (Beijing, China) and 150 bp paired-end reads were generated.