library_construction_protocol: RNA was extracted using TRIzol reagent (ThermoFisher). Poly A RNA was isolated using Dynabeads mRNA DIRECT Purification Kit (ThermoFisher). Ribosome RNA was depleted by RiboMinus Eukaryote Kit (ThermoFisher). Libraries were prepared with SMARTer Stranded Total RNA-Seq Kit v2 (Takara) following the manufacturer's instructions.