library_construction_protocol: Tumors were dissociated mechanically using sterile scissors, then enzymatically using Tumor Dissociation Kit (mouse, Miltenyi Biotec; 130-096-730). Cells were isolated on a 10X Genomics Chromium Instrument. Single cell libraries were generated using the Single Cell 3' Reagent Kit v3.1 chemistry following the guidelines of the 10X Genomics user manual (10X Genomics, Leiden, The Netherlands). Cell number and viability were determined by a Trypan Blue exclusion assay on a Buerker Chamber. Single cell suspensions with 90 percent of viable cells (or single nuclei suspensions) were processed on the 10X Genomics Chromium iX targeting 6000 captured cells per sample. Full length cDNA amplification was carried out using 12 cycles of PCR. Dual index library construction was performed with 13 cycles during sample index PCR.  Final library quantification and quality control were completed using Bioanalyzer 2100 (Agilent Technologies, Les Ulis, France).