library_construction_protocol: After cell lysis, impurities removal, and inhibition of RNAse activity, total RNA was extracted by using the phase separation method from cell debris. Then Agilent bioanalyzer 2100 was used to check RNA integrity and concentration. Sequencing libraries were generated using NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations. mRNA is captured with the PolyA enrichment method.