library_construction_protocol: To prepare sequencing libraries DNA fragments after tagmentation reaction were subjected  to an on-bead PCR amplification with a  total  of 12 PCR cycles using indexing primers (TruePrep Index Kit V2 for Illumina, Vazyme). Post-amplification libraries were size selected at 200-700 bp using KAPA Pure Beads from Roche. Libraries were validated using the Agilent High Sensivity DNA Kit.