library_construction_protocol: Total RNA was extracted using Trizol (Invitrogen, USA) from stationary phase Coccomyxa subellipsoidea cells (WT and CsubMADS1 OX, three biological replicates each) as per the manufacturer's protocol. RNA sequencing libraries were prepared with Illumina-compatible NEB Next® Ultra™ II Directional RNA Library Prep Kit (New England Bio Labs, USA) following manufacturer's instructions.