library_construction_protocol: Total RNA was isolated using the  Qiagen RNeasy® Plus Kit, according to the manufacturer's protocol. RNA concentrations were measured using the the DeNovix DS-11FX spectrometer. 500 ng of RNA was was prepared for sequencing using the KAPA RNA HyperPrep Kit with. Libraries were sequenced on the Illumina NextSeq 500, generating an average of 15–20 million reads per sample.