library_construction_protocol: 3 week old Arabidopsis thaliana seedlings were grown on Â½ MS media containing 1% sucrose and kept in the dark for 48 hours prior to harvesting. Approximately 10 g of tissue. Prior to dark growth, plants were grown at 23 oC under 16 hours of light in controlled environment chambers. Seedlings were grown on plates for 3 weeks. DNA was extracted as in Naish et al., Science 2021 DNA was converted into an Oxford Nanopore sequencing library using kits.