library_construction_protocol: For ribosome profiling, frozen liver tissues from individual mice were homogenized with 5-6 strokes using a Teflon homogenizer in 3 volumes of polysome buffer (150 mM NaCl, 20 mM Tris-HCl pH7.4, 5 mM MgCl2, 5 mM DTT, complete EDTA-free protease inhibitors (Roche) and 40 U/ml RNasin plus (Promega)) supplemented with 1% Triton X-100 and 0.5% Na deoxycholate. Liver lysates were incubated on ice for 10 min and cleared by centrifugation at 10000g, 4°C for 10 min. For each sample, 15 OD260 of liver lysate was digested with RNase I (Ambion) for 45 min at RT under gentle agitation and passed over size exclusion columns for ribosome purification prior to RNA extraction and library preparation. Libraries from ribosome-protected fragments (RPF) and total RNA were prepared as described in Janich et al., 2015 with few modifications. 1 µg of RPF and total RNA samples were depleted of ribosomal RNA using RiboCop rRNA depletion kit V1.3 (Lexogen). Subsequent steps were performed according to Illumina TruSeq Ribo-Profile protocol.