library_construction_protocol: The Chromatin immunoprecipitation (ChIP) assay was performed according to manufacturer instructions with SimpleChip Plus sonication chromatin IP kit (Cell Signaling Technology (CST), Cat. No. 56383) in Jurkat T cells or mouse T cells after treatment with DMSO or plerixafor for 48h, and T cells isolated from the spleens of tumor-bearing mice as described above. For each ChIP, 5106 cells were used. Briefly, cells were cross-linked with 1% methanol-free formaldehyde and quenched by 125mM glycine, and then the chromatin was sonicated to generate 500-800bp DNA fragments. The DNA-protein supernatant was used for immunoprecipitation by incubating with TOX (CST, Cat. No. 5638336778) or p-STAT3 (CST, Cat. No. 9145). Subsequently, the chromatin was eluted and the cross-link was reversed The DNA libraries were prepared using TruSeq ChIP library preparation kit (Illumina) and sequenced on NovaSeq 6000