library_construction_protocol: On day 6 of CTX-induced skeletal muscle regeneration, TA muscles of both hindlimb legs were dissected, digested, and subjected to flow cytometry. After exclusion of doublets and dead cells, myogenic progenitor cells were sorted based on the expression of alpa7 integrin and the exclusion of CD45/CD31/sca1. Subsequently, single cell suspension was generated using Chromium. Chromium Single Cell 3' Reagent Kits User Guide (v3.1 Chemistry).