library_construction_protocol: Total RNA from S2R+ cells was isolated with Trizol (Invitrogen). 3 µg of total RNA were DNAsed with DNase I treatment (New England Biolabs). RNA was directly ribodepleted with the riboPOOL kit (siTOOLs) that was completed with oligos hybridising to 5S and 2S rRNAs following the manufacturer's instructions. TGIRT-seq (Boivin,V. 2018)