library_construction_protocol: Approximately 2 × 10^8cells were collected by centrifugation, suspended in TRIzol reagent (from Invitrogen), and subjected to mechanical lysis by bead beating at 4°C with 0.5-mm glass beads for 1 min, followed by a 2-min rest, for a total of 5 cycles. Following lysis, total RNA was extracted according to the manufacturer's instructions. Residual DNA was removed from the RNA preparation by treatment with the Turbo DNA-free kit (from Ambion) according to the manufacturer's instructions. We used the NEBNext Ultra Directional RNA Library Prep Kit for Illumina protocol according to the manufacturer's instruction. NEBNext Ultra Directional RNA Library Prep Kit for Illumina