library_construction_protocol: Each sample was obtained from the epidermis obtained from 5 larvae (L3).  RNA was isolated using Trizol and 2ug for each were used to construct the library Libraries were prepared using TruSeq RNA sample preparation kit (Illumina) with 2ug of total RNA and  Poly A + selection. A total of 35-45 million reads per sample were obtained using paired-end 100 pb read length (Illumina NovaSeq 6000)