library_construction_protocol: Approximately 1 x 105 cells of each sample were collected and analyzed with Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD903-02) following the recommended protocol. Briefly, harvest fresh cultures and count 1 x 105 cells for each sample. Then bind cells to Concanavalin A beads at room temperature for 10 min. Next, remove liquid and add primary antibody with ice-cold Antibody Buffer and incubate overnight at 4 oC. The next day, remove liquid and add Guinea Pig Anti-Rabbit Antibody secondary antibody with Dig-Wash Buffer and incubate at room temperature for 1 h. Then, wash sufficiently and bind CUT&Tag pA/G-Tn5 Transposomes at room temperature for 1 h. After gently but throughly wash, tagmentation at 37 ℃ for 1 h and then performed DNA extraction with DNA extraction beads. For extracted DNA, combinate i7 and i5 Indexed Primer for PCR amplification and perform clean-up for quantification and Illumina sequencing.