library_construction_protocol: Tissue was dissociated using 6.6 mg/ml Bacillus Licheniformis protease, 5 mM CaCl2 and 20 U/ml DNase I, where the mixture was triturated on ice for 20 s every 5 minutes until clumps of tissue were no longer visible. Chromium Single Cell 3' Library Construction Kit v3 and v3.1 (10x Genomics)