library_construction_protocol: Freshly dissected right mouse brain hemisphere was flash frozen in OCT using a set-up of isopentane and liquid nitrogen (as per 10xGenomics Visium Spatial Gene Expression protocols). Fresh frozen tissue was directly sectioned onto Visium Spatial Gene Expression Slides at 10μm using a cryostat. Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's instructions