library_construction_protocol: Frozen leaf tissue was ground to a fine powder in liquid nitrogen-cooled mortar and pestles. Lysate buffer was added to the tissue (350 µL per 100 mg of tissue) and the mixture was vortexed immediately. RNA was extracted using ISOLATE II RNA Plant Kit (Meridian Bioscience). RNA from two individual leaf samples was equally pooled to provide two replicates for sequencing. Library preparation and RNA-sequencing was performed by a commercial provider (Azenta). Library preparations were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs; NEB).