library_construction_protocol: The total RNA was extracted using miRNeasy Micro Kit (Cat#217084, Qiagen), and RNA quality was checked using an Agilent Bioanalyzer 4200 (Agilent technologies, Santa Clara, CA, US). Sequencing libraries were generated using VAHTS® Universal V6 RNA-seq Library Prep Kit for Illumina (NR604, Vazyme, Nanjing, China) according to the manufacturer's instructions.