library_construction_protocol: RNA was extracted from cell pellets using the NucleoSpin Plus RNA isolation kit (Macherey-Nagel, Düren, Germany). The integrity and concentration of RNA samples was assessed using the TapeStation 2200 system (Agilent, Santa Clara, California) Library preparation was carried out with the Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA).