library_construction_protocol: RNA samples were extracted by  Trizol reagent (Takara#9108Q) and Direct-zol RNA MiniPrep Kit (Zymo#R2052). 3ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-Seq were prepared using VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme #NR605-01).