library_construction_protocol: cDNA library construction and mRNA-sequencing were entrusted to Bioengineering Lab. Co., Ltd. (Sagamihara, Japan). Total RNA concentration was measured by Quantus Fluorometer and QuantiFluor RNA system (Promega). RNA quality was checked by 5200 Fragment Analyzer System and Agilent HS RNA Kit (Agilent Technologies). Using MGIEasy RNA Directional Library Prep Set (MGI Tech Co Ltd.), library construction was performed. The concentration of the library was analyzed by Qubit 3.0 Fluorometer and dsDNA HS Assay Kit (Thermo Fisher Scientific). The library quality was checked by Fragment Analyzer and dsDNA 915 Reagent Kit (Advanced Analytical Technologies). cDNA was made from the library usingMGIEasy Circularization Kit (MGI Tech Co Ltd). DNA nanoball (DNB) was made using DNBSEQ-G400RS High-throughput Sequencing Kit (MGI Tech Co Ltd.). DNB was sequenced using the DNBSEQ-G400 paired end sequencing (2x100 bp).