library_construction_protocol: Dissected brain tissue was dissociated using papain tissue dissociation kit (Worthington) following manufacturer's protocols. Single cells were check for viability using LiveDead stain to ensure greater than 90% viability before proceeding to loading into GEMs via the 10x genomic Chromium controller. scRNA-seq were prepared using 10x Genomics Chromium v3 Library Prep Kit following manufacturer's protocols. 10x genomic chromium 3' kit v3.1 library