library_construction_protocol: hMDMs were infected with lentivirus carrying control or Rfx1-3×Flag plasmid and stimulated with LPS for 24 hours.  Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated.Before data analysis, the original sequencing data were processed to remove the low-quality and splitter sequences using Skewer software. The Fastqc software was used to perform quality control of the treated sequences. The filtered sequencing data were compared with the reference genome (hg38) by Bowtie to obtain alignment information of ChIP-seq. ChIP-seq libraries were prepared using VAHTS Universal DNA Library Prep Kit for Illumina