library_construction_protocol: RNA was harvested using Total RNA Purification Kit (NorgenBiotek). 2ug of RNA was used for library contruction. RNA was converted into cDNA using TeloPrime Full-Length cDNA Amplification Kit V2 following manufacture's protocol. The resulting cDNA was used for library contruction with the 2S DNA turbo kit from Swift Biosciences followinf manufacture's protocol