library_construction_protocol: The extraction, reverse transcription, preamplification of total RNA from mice heart tissues (Wt and Mcm8-/-) were conducted using Discover-scTM WTA Kit V2 (Vazyme). The quantity and quality of RNA were assessed by the QubitRNA assay kit on Qubit3.0 Fluorometer (Life technologies, CA, USA) and RNA Nano 6000 Assay kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA), respectively RNA libraries were constructed using Discover-scTM WTA Kit V2 (Vazyme) according to the manufacture's instructions.