library_construction_protocol: Purified total RNA from tumors was extracted by total RNA extraction kit (TIANGEN, Cat. No. DP419) according to the manufacturer's instructions and analyzed by an Agilent Bioanalyzer to assess RNA integrity. Sequencing libraries were prepared using a VAHTS Stranded mRNA-seq Library Pre Kit (Vazyme, NR602-02) as the manufacturer's protocol. RNA Sequencing was performed on the Illumina NovaSeq 6000 platform.