library_construction_protocol: Genomic DNA samples were sheared to ~2 kb in a total volume of 200 µl using a Covaris miniTube Clear on the Covaris M220 with shearing conditions as follows: Peak Incident Power: 8 W, Duty Factor: 20%, Cycles per Burst: 1000, Treatment time: 900 s. Sonicated DNA was purified using AMPure XP beads (Beckman-Coulter) at a 1:1 ratio, eluted in 22.5 µl AMPure Elution Buffer and checked for quality using the DNA12000 assay in an Agilent BioAnalyzer. SMRTbell libraries were prepared using PacBio barcoded adapters according to the manufacturer's instructions. PacBio long-read SMRT sequencing