library_construction_protocol: Total RNA for mRNA sequencing was extracted using QIAGEN RNeasy mini kit. To separate nuclear and cytoplasmic fractions, pellet the cells via centrifugation for 5 minutes at 1500RPM and discard the supernatant. The pellet was then resuspended into 300mL of ice cold 0.25x lysis buffer (Tris-Cl pH 8.0, NaCl, MgCl2, Nonidet P-40). The pellet was kept on ice for two minutes and rotate the tube every 45 seconds to ensure proper lysis followed by centrifugation at highest speed at 4°C for two minutes. After centrifugation, the supernatant was carefully removed and placed in a new tube as this was the cytoplasmic fraction. For the remaining nuclear fraction pellet, 500mL of ice-cold PBS was added and centrifuged at 1500RPM for 5 minutes. The QIAGEN RNeasy mini kit was then used for long RNAs and miRNeasy Tissue/Cells Advanced Micro Kit was used for small RNA extraction. For mRNA sequencing was Poly(A)-selected and the Illumina libraries were prepared using the TruSeq stranded mRNA Library Preparation kit. For the nuclear cytoplasmic mRNAs, ribsomoal RNA depeletion method with the TruSeq stranded Total RNA library was used with 50 million paired end reads. For the nuclear cytoplasmic small RNAs, the size selection step with the Perkin Elmer Next Flex Small RNA library preparation kit on the Illumina sequencing platform with 50 million single end reads.