library_construction_protocol: Human venous peripheral blood samples were taken and collected in ethylene diamine tetra-acetic acid (EDTA) tubes by venipuncture. Diluted blood was layered on top of Ficoll-Paque Plus and peripheral blood mononuclear cells were separated by centrifugation at 400 × g for 30 minutes at room temperature Libraries were performed following Chromium Next GEM Single Cell 3' protocol from 10xGenomics. GEMs are generated in Chromium Controller, followed by RT and cDNA amplification. 3' Gene Expression Library construction comprises of the following steps: fragmentation, end repair, A-tailing, adapter ligation, sample index PCR. Sequencing-ready libraries are checked for their quality on Bioanlayzer DNA chip.