library_construction_protocol: Frozen aortas were homogenized in lysis buffer (PeqGOLD total RNA isolation Kit, Thermo Fisher Scientific, Waltham, MA, US) using a Precellys® homogenizer (VWR®, Randnor, Pennsylvania). Total RNA was isolated according to the manufacture's protocol and the RNA concentration was determined with a NanoDrop (VWR®, Vienna, Austria). The reverse transcription was performed using a RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Waltham, MA, US). Real-time PCR analysis was performed with ~10–30 ng of cDNA in a StepOnePlusTM Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). Gene expression levels were quantified using specific primers and SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, Hercules, CA, US). Sequencing libraries were prepared at the Core Facility Genomics, Medical University of Vienna using the NEBNext Poly(A) mRNA MagneticIsolation Module and the NEBNext Ultra™ Directional RNA Library Prep Kitfor Illumina according to manufacturer's protocol (New EnglandBiolabs). Libraries were QC‑checked on a Bioanalyzer 2100 (Agilent) using a HighSensitivity DNA Kit for correct insert size and quantitated using QubitdsDNA HS Assay (Invitrogen). Pooled libraries had an average length of 330‑360 bp and were sequenced on a HiSeq3000/4000 instrument (Illumina) in 1x50bp sequencing mode