library_construction_protocol: RNA was harvested from frozen tissues using Chemagic 360 instrument (PerkinElmer) and quantified Libraries were prepared using Lucigen NxSeq AmpFREE Low DNA Library Kit (Lucigen) and bisulfite-converted using Zymo EZ DNA-Methylation Gold Kit (Zymo Research), and PCR amplified using KAPA HiFi Uracil PCR Kit (Kapa Biosystems).