library_construction_protocol: RNA was extracted from intact brain tissue or from ischemic lesions 14 days after MCAO using RNeasy Micro Kit Qiagen (Qiagen, USA) following the manufacturer's protocol and stored at −70 °C. cDNA libraries were constructed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA).