library_construction_protocol: Cells were collected and resuspended in 700uL of RLT buffer (Mini RNeasy Kit) and homogenized using TissueRuptor (Qiagen). RNA was then isolated using the RNeasy Mini Kit (Qiagen) as per manufacturer's instructions, DNase treatment was performed using RNeasy DNase kit (Qiagen) and protocol cDNA libraries were prepared with the Ovation Ultralow RNA-seq System V2 (NuGEN)