library_construction_protocol: Single live CD31+ cells from the retina were enriched using CD31 microbeads. The cell suspensions were mixed with filtered trypan blue to evaluate cell viability and concentration. we performed the following steps on a 10x Genomics chromium single-cell platform, and libraries were constructed according to the manufacturer's instructions of 10x Chromium Single Cell 3′ Regent Kit v3 protocol (10X Genomics, 1000121).