library_construction_protocol: Total RNA was extracted from the brain section containing hippocampus and cortex using RiboPure™ RNA Purification Kit (ThermoFisher Scientific). Ribosomal RNA was removed using Ambion RiboMinus Eukaryote System. RNA concentration and integrity were checked using an Agilent Bioanalyzer (RIN>7). Libraries were prepared using Ion Total RNA-Seq Kit for the AB Library Builder System