library_construction_protocol: RNA was isolated from cell pellets using the QIAGEN RNEasy Mini kit according to the manufacturer's instructions. PolyA RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module First-strand Illumina-barcoded ibraries were constructed using the NEBNext Ultra II Directional RNA Kit. During the library construction Ilumina R2 primer sequence, P7 sequences and a sample index were added.