library_construction_protocol: (B220-, CD19-, CD11b-, CD11c-, NK1.1-, TER119-, TCRβ-, TCRγ/δ-) DN thymocytes were sorted from Rag1-/- mice. RNA sequencing library preparation was prepared using Rib0-Zero rRNA Removal Kit and TruSeq Stranded Total RNA library Prep kit following manufacturer's protocol (Illumina, RS-122-2101)