library_construction_protocol: Five mm liver tissue samples were rinsed with phosphate buffered saline (PBS), embedded in optimal cutting temperature (OCT) medium (Tissue-Tek®), and snap frozen in liquid nitrogen-cooled isopentane. Samples were stored in −80°C prior to use in the spatial transcriptomics workflow. Spatial transcriptomics libraries were prepared using the Visium Spatial Tissue Optimization slide and reagents from 10x Genomics.