library_construction_protocol: Total RNA isolation Enrichment of mRNAs with the NEBNext Poly(A) mRNA Magnetic Isolation Module was followed by library preparation using the NEBNext Ultra II RNA Directional Library Prep Kit for Illumina (New England BioLabs). Single read sequencing took place on a NextSeq 2000 System (Illumina), using the corresponding NextSeq 2000 P3 Reagents (50 cycles), with a read length of 72 base pairs.