library_construction_protocol: RNA was isolated using Trizol and RNA Clean & Concentrator Kits (Zymo Research). 1.5 ug of total RNA was used for the construction of sequencing libraries Standard miRNA libraries were generated using the NEXTFLEX small RNA-seq kit v3 (Perkin Elmer, Waltham, MA, USA), and single-end libraries were sequenced on an Illumina SE75 Platform