library_construction_protocol: CCL86 Raji and HTB96 U2OS cells were cultured for 80% confluency for RNA-seq assay. Half of the cell cultures were treatment with doxorubicin and the other half remained untreated. We used 150 ng/ml concentration of doxorubicin for U2OS cells and 200 ng/ml for Raji cells. The multiple culture plates were pooled and then divided into six separate Petri dishes for determining transcriptional profil in each cell line. Total RNA was extracted using min-plus kit from Qiagen. Around 10ug total RNA was used for the sequencing libraries. Illumina HiSeq2500 platform in a 2x100bp paired-end configuration was used to obtain 35 million reads on average for each library sample