library_construction_protocol: Collection of 3 million cells in TRIzol RNA was isolated from VCaP cells in four independent replicates, marked with Illumina total prep 96 RNA amplification kit (Ambion). RNA sequencing was performed using NEBNext Ultra Directional RNA Library Prep Kit for Illumina starting from total RNA and sequenced on the Illumina NextSeq2000 with single-end, 120 base pair long reads.