library_construction_protocol: Fungal material was harvested by scrapping the surface with a razor blade. Samples were collected before the crossing (2 and 1 day prior fertilization), right before crossing (day 0) and 1, 2, 4, and 6 days after fertilization. All tissues and perithecia were immediately frozen in liquid nitrogen and stored at -80°C. Poly-A selection and cDNA libraries preparation for RNA were prepared according to the Illumina mRNA Sample Preparation Guide