library_construction_protocol: After 3 days, interscapular BAT of WT and Ucp1-/- mice was harvested, and total RNA extracted by using an RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's instructions. Total RNA was assayed for quantity and quality with an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries of coding and small non-coding RNAs were generated using TruSeq Stranded mRNA HT Sample Prep Kit (Illumina) as per standard protocol in the kit's guide. A MiSeq micro test lane was run to check pool balance followed by 75 bp single-read sequencing on an Illumina HiSeq 2000 sequencer.