library_construction_protocol: LNCaP/AR cells transduced with Caspase 9 and different sgRNA constructs were treated with enzalutamide or vehicle for 6 days before the total RNA was extracted using Trizol. RNA-Seq libraries were prepared using the Illumina TruSeq stranded mRNA kit, with 10 cycles of PCR amplification, starting from 500 ng of total RNA.