library_construction_protocol: Collected day 10 aggregates from the 3 iPSC lines and disassociated them by treating with room temperature AccuMax™. Then processed cells for scRNA-seq using the 10X Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index). 3' gene expression dual index library construction (10x Genomics).