library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 100ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared usingRoche Kapa RNA Hyper prep with Riboerase (HMR) for Illumina platforms, version KR1351 – v4.21 (Cat. #: 8098140702)