library_construction_protocol: embryos were lysis in 100 μl wash buffer (20 mM HEPES pH 7.5; 150 mM NaCl; 0.5 mM Spermidine; 1x Protease inhibitor cocktail) The protocol for the CUT&Tag library was adapted from a previous report with minor modifications (Kaya-Okur et al., 2019)