library_construction_protocol: Bone marrow flushed tibias were stored in RNAlater (Sigma-Aldrich, R0901-500ML) overnight at 4ºC before removing solution and storing at -80ºC until ready for processing. Frozen bones were ground with a mortar and pestle in liquid nitrogen and bone dust was homogenized in bead mill tubes (FisherBrand, 15-340-151) in Trizol (Thermo Fisher, 15596026). RNA was extracted using RNEasy Mini Kit (Qiagen, 74104). RNAseq library was prepared (Illumina stranded mRNA prep) and performed on Nextseq 2000 P3 – 200 cycles (up to 1.1 billion reads) (Illumina).