library_construction_protocol: RNA from 10,000 sorted nuclei was extracted from TRIzol with the Direct-zol RNA Microprep Kit (Zymo), and the RNA from each sample was eluted in 10 μl of RNase-free water. The Smart-seq V4 Kit (Takara) was then used for RNA-seq library preparation. The RNA was then used for cDNA generation with nine PCR cycles to amplify the cDNA. After measuring the cDNA concentration (Qubit dsDNA HS Assay Kit, Thermo Gisher Scientific) and quantifying control with Bioanalyzer 2100 (Bioanalyzer High Sensitivity DNA Analysis, Agilent), 300 ng of cDNA was used for library preparation with the Nextera XT DNA library preparation Kit (Illumina).