library_construction_protocol: Total RNA was isolated from each sample using the Nucleospin RNA Plant and Fungi Mini kit (Macherey-Nagel, PA, USA) following the manufacturer's protocol. To remove all DNA, the total RNA was treated with DNase using the PureLinkTM Dnase Set (InvitrogenTM, CA, USA). To selectively precipitate RNA, a lithium chloride (LiCl) precipitation step was performed RNA-seq libraries were constructed at the ICBR Gene Expression and Genotyping Core Lab using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, United States) following the manufacturer's recommendations. A total of 1000 ng samples of RNA with RNA integrity numbers (RIN) greater than 5.6 were used