library_construction_protocol: Fresh frozen tissue is embedded with OCT and 10um frozen sections were sliced on the cyrostat.  The left hemisphere of the mouse brain is placed on tissue slide at bregma -1.8. Library was performed according to manufacturers instructions 10x Genomics at the Biomedical Research Center core sequencing facility at the University of British Columbia, Vancouver, Canada. Tissue was permeabilised for 18 minutes according to Visium Spatial Tissue Optimization slide & reagent kit.