library_construction_protocol: 1 μg of total RNA was isolated using TruSeq Stranded Total RNA Library Prep Kit (Illumina). The libraries were prepared using TruSeq Stranded mRNA Sample Prep Kit (Illumina). mRNAs were fragmented and synthesized as double-stranded cDNA through random hexamer priming. Following the end repair, A-tailing, and adapter ligation, cDNA libraries were amplified with PCR. The quality of cDNA libraries was evaluated with Agilent 2100 BioAnalyzer (Agilent Technologies). The quantification of cDNA libraries was conducted using KAPA library quantification kit (Kapa Biosystems). Following cluster amplification, RNA-seq progressed as paired-end (2×150bp) using Illumina Novaseq6000 (Illumina).