library_construction_protocol: Total RNA was extracted from flash frozen tissue samples using Qiagen miRNeasy kits.  20mg of frozen liver was placed in pre-filled tube containing Qiazol reagent and Zirconium beads, then homogenized 2 x for 20 seconds.  Subsequent steps were followed as per manufacture's instructions. 3-methylfuran cDNA libraries were constructed using Illumina TruSeq Stranded mRNA protocol. Furan cDNA libraries were prepared with the Illumina Stranded mRNA prep kit (updated release).