library_construction_protocol: Total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies), and the concentration, quality and integrity of total RNA were determined using a NanoDrop spectrophotometer (Thermo Scientific). Three micrograms of RNA were used as input material for the preparations of RNA sample. Sequencing libraries were constructed using the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).