library_construction_protocol: On day 8 of culture, embryoid bodies were dissociated with 0.25% Trypsin-EDTA for 8 minutes and Collagenase II for 30 minutes, immunolabled at 4C for 25 minutes. CD34+CD43–CXCR4–CD73– hemogenic endothelial cells were then FACS sorted using a BD FACSMelody and stored on ice until ready for library preparation. scRNA-Seq libraries were prepared according to the manufacturer's instructions