library_construction_protocol: Frozen bone marrow samples were thawed and transferred into 50ml conical tubes containing 1x PBS +2% fetal bovine serum(FBS). Cell suspensions were centrifuged at 300 x g for 5 minutes at 4℃, and supernatant was removed. Samples were then enriched for >90% alive cells by using a dead cell removal kit, or stained with DAPI before sorted for alive cells using SY3200 Cell Sorter (SONY). For cell sorting, DAPI low cells are collected into 15ml conical tubes containing 3ml FBS on ice. Samples were then centrifuged at 300 x g for 5 mins at 4℃. Enriched alive cells were first stained with cell-hashing oligo-tagged antibodies(Biolegend)  according to manufacturer's instructions. Then samples were counted and pooled together and stained with a CITE-seq antibody cocktail (Biolegend) according to the manufacturer's instructions. After staining, samples were processed using 10X Genomics Chromium controller with the Single Cell Gene Expression 3' Reagent Kits Library was prepared using Chromium Single Cell 3ʹ v3.1 protocol (10x Genomics). Cellular mRNA and antibody-derived oligos were reverse-transcribed and indexed with a shared cellular barcode. Indexed cDNAs were then pooled and amplified by PCR according to the Chromium Single Cell 3' v2 protocol (10x Genomics) and with specific primers amplifying the antibody-derived tags. SPRI bead size selection was performed in order to separate both the mRNA-derived cDNA (>300bp) and the antibody-derived tagged cDNAs (180bp). For the mRNA derived cDNA library preparation, we further proceeded according to the manufacturer's instructions for Single Cell 3' v2 protocol (10x Genomics). For antibody-derived tagged library, we used the KAPA HiFI HotStart Library Amplification Kit (Roche) with the following primers and amplification program: CITE-seq library: amplification was performed at 95°C for 3' followed by ten cycles at 95°C for 20''; 60°C for 30''; 72°C for 20'' and final elongation 72°C for 5'. HASH-seq library: amplification was performed at 95°C for 3' followed by ten cycles 95°C for 20''; 64°C for 30''; 72°C for 20'' and final elongation 72°C for 5'. Following the final bead purification, all three libraries were pooled as 80% mRNA library, 15% ADT library and 5% HTO library before sequencing.