library_construction_protocol: Microbial community DNA was extracted from the feces of animal models using the QIAamp Stool DNA Kit (Qiagen) according to the manufacturer's instructions. DNA was quantified using a Qubit dsDNA HS Assay kit (ThermoFisher Scientific).The V4 region of the bacterial 21S rRNA gene was amplified using the following degenerate primersreverse primers.