library_construction_protocol: Total RNA was isolated from archival serum samples using the miRNeasy kit (Qiagen, Germantown, MD) according to the manufacturer's instructions small RNA-seq libraries were prepared by using the Norgen Biotek Small RNA Library Prep Kit (Norgen Biotek, Therold, Canada), and sequenced on the Illumina NextSeq 500 platform. NextSeq 500/550 High Output Kit v2 (51 Cycles using a 75-Cylce Kit)