library_construction_protocol: Total RNA was extracted from individual samples and controls using Trizol reagent (Invitrogen) according to the manufacturer's protocol. Total RNA was then quantitated using a Nanodrop spectrophotometer (Thermo Scientific) and quality assessed using the RNA 6000 Nano assay kit (Agilent) and Bioanalyser 2100 (Agilent). NGS sequencing libraries were generated from 1 µg of total RNA using a TruSeq RNA Sample Prep Kit (Illumina) according to the manufacturer's protocol. The resulting cDNA libraries were then paired-end sequenced (2 × 101 bp) for samples with the Novaseq 6000 system (Illumina).