library_construction_protocol: Multicellular suspensions were recovered and stained with directly labelled antibody cocktail including a-CD45-AF700 (clone 2D1), a-CD3-PerCP (clone SK7), a-CD56-APC (clone CMSSB), a-CD16-efluor780 (clone CB16), a-CD103-FITC (Clone B-Ly7) and DAPI as a viability die. Total RNA was isolated (RNeasy« Micro Kit, 74004, Qiagen) from purified NK cells and RNA-sequencing (RNA-seq) of bulk NK cell subsets was performed by the Genomics UPF facility at Universitat Pompeu Fabra, Barcelona. 500 ng of each sample were used to prepare NGS libraries with the NebNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs) with PolyA capture.