library_construction_protocol: Total RNA was extracted from tissue samples, and the concentration and purity of the extracted RNA were detected by Nanodrop2000, RNA integrity was detected by agarose gel electrophoresis, and RIN value was determined by Agilent2100. The total amount of RNA required for a single library construction is ≥1ug, the concentration is ≥35ng/μL, OD260/280 is ≥1.8, and OD260/230 is ≥1.0. RNA libraries for RNA-seq were prepared using Illumina TruseqTM RNA sample prep Kit following manufacture's protocols.