library_construction_protocol: Tumors were resected in ice-cold PBS and dissociated using tumor dissociation buffer (DMEM with 10%FBS, 1 mg/ml collagenase II, 2mg/ml dispase II and 100K unit/ml DNase I). Tissues were mechanically dissociated using a cell strainer pestle and incubated in 37°C incubator for 1 hour. A single-cell suspension was obtained by filtering the tissue lysate through a 70 μm cell strainer. The single-cell suspension was then resuspended in red blood cell lysis buffer. After centrifugation, cells were resuspended in FACS buffer and stained for V450-CD45 for cell sorting. CD45+ cells were sorted out and kept in PBS for library preparation. The single-cell gene expression Library was prepared according to the Chromium Single Cell Gene Expression 3'v3.1 kit (10x Genomics). Briefly, single cells, reverse transcription (RT) reagents, Gel Beads containing barcoded oligonucleotides, and oil were loaded on a Chromium controller (10x Genomics) to generate single-cell GEMS (Gel Beads-In-Emulsions) where full-length cDNA was synthesized and barcoded for each single cell. scRNA-seq, sample multiplexed by using Cellplex