library_construction_protocol: PBMC was obtained using ficoll-hypaque gradient centrifugation in accordance with the manufacturer's instructions. Biopsies were cut into small fragments and incubated at 37°C in buffer containing 0.25% trypsin (Gibco) and 40 U/ml DNase I (Thermo Fisher). Dissociated cells were filtered every 10 minutes through a 70 mm cell strainer (Greiner Bio-One) into cold 10% FBS buffer. Residual tissue was dissociated again and passed through the cell strainer. After centrifugation at 400Xg for 5 minutes, cells were resuspended in 2% FBS buffer and strained again through a 40-μm cell strainer Single cell Gene Expression Library was prepared according toChromium Next GEM Single Cell 3' Reagent Kits v3(10x Genomics). In Brief, single nuclei suspension, reverse transcription (RT) reagents, Gel Beads containing barcoded oligonucleotides, and oil were loaded on a Chromium controller (10x Genomics) to generate single cell GEMS (Gel Beads-In-Emulsions) where full length cDNA was synthesized and barcoded for each single cell. Subsequently the GEMS are broken and cDNA from each single cell are pooled. Following cleanup using Dynabeads MyOne Silane Beads, cDNA is amplified by PCR. The amplified product is fragmented to optimal size before end-repair, A-tailing, and adaptor ligation. Final library was generated by amplification.