library_construction_protocol: The total RNA was extracted from pre-and post-fertilized samples using TRI-reagent. The concentration and its purity were checked on the nanodrop 2000 Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE). The quality of RNA samples was also cross checked bt RNAase free agarose gel electrophoresis and bioanalyzer (BIO-RAD, USA). Best quality RNA samples with 260/280 ratio and RNA integrity number (RIN≥7) were further selected for transcriptome sequencing. Total RNA was used for library preparation and paired end sequencing was performed using ILLUMINA platform according to manufacturer's instruction.