library_construction_protocol: Nuclei were isolated from the frozen superior temporal cortices as described previously with some modifications (Jakel, S. et al., Nature, 2019). Briefly, the frozen tissue samples were briefly exposed to 2 mL of Nuclei EZ Lysis Buffer (Sigma), supplemented with EDTA-free protease inhibitors (Sigma) and recombinant RNase inhibitors (Takara Bio USA) at 4°C. The tissue was then homogenized using a glass Dounce tissue grinder through 10-15 strokes with pestle A, followed by 10 strokes with pestle B. Then, an additional 2 mL of lysis buffer containing protease and RNase inhibitors was added to the homogenized tissue, followed by 5-minute incubation. Subsequently, the nuclei suspension was filtered using a 40-μm cell strainer and pelleted by centrifugation at 300 x g for 5 minutes. The nuclei pellets were resuspended in 900 μL of debris removal solution (Miltenyi Biotech) containing 0.4 U μL−1 RNase inhibitors and 3.1 mL of calcium and magnesium-free Dulbecco's phosphate-buffered saline (DPBS). Next, 4 mL of DPBS was carefully overlayed onto the nuclei resuspension was performed, followed by another centrifugation at 3,000 x g for 5 minutes. Post-centrifugation, the supernatant was discarded, and the nuclei were resuspended in the solution consisting of 1% BSA in DPBS, supplemented with 0.4 U μL−1 RNase inhibitors. Throughout the process, the nuclei were kept on ice or at 4°C. Lastly, the density of the nuclei suspension was calculated using a C-chip-disposable hemocytometer (INCYTO) with trypan blue staining (Bio-Rad). Chromium platform (10x Genomics)