library_construction_protocol: For RNA-sequencing on CD103+ CD11b- CD8a+ mLN DCs, 1,000 CD103+ CD11b- CD8a+ DCs were FACS sorted into a 96-well plate containing 12.5 µl of lysis buffer and cDNA was generated using the Takara Smart-Seq HT Kit (Takara, 634438) following manufactures instructions, with 15 cycles of cDNA amplification. Samples that passed QC with full length cDNA proceeded to library preparation using Illumina Nextera DNA prep (Illumina, 20018705) with UDI indexes (Illumina, 20027214) added using 8 PCR cycles.