library_construction_protocol: Spleens were removed from euthanized mice, homogenized using 70µm cell-strainer and single cell suspension was then filtered through 40µm cell-strainer. Splenocytes were pelleted by centrifugation at 500 × g for 5 min. To lyse the red blood cells, the cell pellet was suspended in 5 mL of 0.14M ammonium chloride solution for 5 min at room temperature. RBC lysis was stopped by adding 10 mL of R10 media (RPMI media supplemented with 10% FBS) and centrifuged at 500 × g for 5 min and resuspended in R10 media at 1x106/mL concentration. Further, the cells were plated in tissue-culture treated 6-well plate and allowed to adhere for 2 hrs. Non-adherent cells were removed and the adherent cells were infected with GFP-Bb at a multiplicity of infection (MOI) of 1:1. 10x Genomics single cell libraries were generated by the University of Texas at San Antonio (UTSA) Genomics Core. Cell suspensions were loaded into 10x Genomics microfluidics chips and onto the 10x Chromium to capture single cells in Gel Beads-in-emulsion (GEMs) reactions. 10,000 cells/sample were targeted for capture in GEMs preceding library prep following manufacture's recommendations for the 3' Gene Expression v3 kit.