GSM7034602
Anti-mesothelin M5 CAR-TCONV and CAR-TRM cells were harvested after nine days of in vitro expansion. CD8+ CAR T-cells were magnetically isolated using anti-mesothelin-coated beads (ACROBiosystems) and anti-CD8 microbeads (Miltenyi Biotec), followed by resuspension in 500µL of TRIzol (Invitrogen). Total RNA was prepared using RNA Clean & Concentrator™ kits (Zymo Research) following the manufacturer's instructions.