GSM7438775
Retinas were surgically removed from euthanized animals and placed in pre-chilled eppendorf tubes. RNA was immediately isolated using the Direct-zol RNA Microprep kit (Zymo Research, Irvine CA, cat#R2062) according to manufacturer protocol and eluted in 15μL of RNAse-free water. RNA integrity and concentration were assesssed on a Nanodrop. Sequencing libraries were generated from 150-230ng of total RNA using Lexogen's Quantseq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Greenland, NH). Libraries were sequenced on a NovaSeq 6000 with a minimum of 5 million reads per sample.