GSM6706776
After euthanasia of the mice, the liver was removed, cut into small pieces and minced using a scalpel. The tissue was then incubated for 30 min at 37°C on a shaking incubator in HBSS (with Ca2+ and Mg2+) with Collagenase (1 mg/ml) and DNase I (10 U/ml) and supernatant was collected. Leukocytes were further enriched by Percoll gradient centrifugation (GE Healthcare, Chicago, IL). Cells were then FACS sorted to purify CD45+ cells and to obtain IL-22-BFP negative and positive fractions. Single Cell 3' v2