GSM7688958
Burkitt lymphoma diagnostic specimens, including pleural or abdominal effusions and nodal tumor masses, were collected at diagnosis and frozen as viable single cell suspensions. Upon thawing, cell suspensions were subject to Ficoll density centrifugation to remove dead cells and diluted at the concentration of 1,000 cells/ul. Single cells were captured and analyzed in a Chromium Controller (10x Genomics) using the Chromium Next GEM Single Cell 5' kit v2 and the Chromium Next GEM Single Cell V(D)J Enrichemtn Human B cell Kit following manufacturer's instructions (10x Genomics).