GSM7781213
RNA was harvested using RNeasy mini kit (Qiagen) and concentrations of total RNA was measured with NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Qubit 4 Fluorometer (Thermo Fisher Scientific -IN). The RNA quality was assessed with the Tapestation 4200TM (Agilent Technologies, Santa Clara, US). The initial input of 500 ng of QC passed RNA were used for construction of stranded mRNA-Seq libraries using KAPA mRNA HyperPrep Kitfollowing manufacturer protocol. RNA-Seq: Illumina libraries were loaded onto Illumina NextSeq2000 to generate 40M, 2 X 150 bp reads/sample.