GSM7438925
Provide samples in well labeled individual eppendorf tubes of, ideally, ~5000-6000 cells each (frozen in BamBanker solution). Wash/lyse/purify during transposition using Buenrostro ATAC protocol. Modified protocol adapted for low yield bulk ATAC from Buenrostro J, et al. (current protocols in molecular biology, 2015) Samples received, lysed and transposed using adapted bulk ATAC protocol. Cleaned using Qiagen MinElute spin columns. QC for amplification using KAPA quant. Libraries barcoded for sequencing using Nextera i5 and i7 primers. Pooled at 4nM and sequenced in house using Illumina Next-seq 75cycle high throughput kit. R1- 38cycles, R2- 38, i1- 8, i2- 0. All QCs done by qubit/KAPA/qPCR