GSM7822998
RNA-protein crosslinking, cell lysis, CoIP using anti-FLAG-antibody, RNA ends trimmed using RNase A/T1, ligation of neighboring RNAs, Protein digestion using Proteinase K, RNA extraction, fragmentaton and DNase digestion, ribosomal RNA depletion and purification with AMPure XP beads first strand cDNA synthesis using SuperScript III and Primer AR2, RNA degradation, cDNA cleanup, ligation of second adaptor(3Tr3) to cDNA 3' ends, cDNA cleanup and PCR enrichment using P5_Enr primer and P7_BC1_Enr primer RIL-seq (doi: 10.1038/nprot.2017.115)