GSM7105812
IRF4-GFP or GFP-control-transduced Pmel-1 cells were used to treat B16-F10 melanoma three days after tumor implantation. Fourteen days after tumor implantation, tumor-infiltrating IRF4-GFP and GFP-control-transduced Pmel-1 cells, as well as endogenous CD45+ TILs from both the IRF4 and control groups, were sorted using a cell sorter. The Single Cell Gene Expression Library was prepared using the Chromium Next GEM Single Cell 3' Reagent Kits v3 (10x Genomics) A single nucleus suspension, reverse transcription reagents, Gel Beads containing barcoded oligonucleotides, and oil were loaded onto a Chromium controller (10x Genomics) to generate single cell GEMs (Gel Beads-In-Emulsions), where full-length cDNA was synthesized and barcoded for each single cell. Subsequently, the GEMs were broken, and cDNA from each single cell was pooled. After cleanup using Dynabeads MyOne Silane Beads, the cDNA was amplified by PCR. The amplified product was fragmented to an optimal size before undergoing end-repair, A-tailing, and adaptor ligation. The final library was generated through amplification, and its quantity was determined using the KAPA Library Quantification kit (Roche)