GSM7230192
RNA was harvested using the Rneasy mini kit (Qiagen). The libraries were generated using MERCURIUS BRB-seq kit (PN 10813, Alithea) following the user guide. In brief, the RNA samples were reverse transcribed using barcoded oligo-dT primers. Illumina libraries were prepared with cDNA following tagmentation protocol and sequencing on the Illumina Nextseq instrument (Alpern et al, 2019). RNA-seq (paired-end, multiplexed, including UMI)