GSM7439483
Plant tissues were fixed in 1x PBS with 1% formaldehyde for 15 min under vacuum and ground to fine power under liquid nitrogen. Nuclei were purified using a one-step Percoll gradient centrifugation and sonicated to obtain DNA fragments of 300–500 bp using a Covaris M220 (Covaris). The reverse crosslinked DNA was end-repaired, dA-tailed, ligated to Y-adapters, and PCR amplified prior to sequencing.