GSM6707788
At least 40 pharate animals per sample were staged. Thoracic muscles were collected and flight muscles were dissected. Nuclei were extracted by mincing tissue with douncer and lysis buffer as in (Buenrostro et al, Curr. Protoc. Mol. Biol, 2015) with some modification. A final concentration of TritonX-100 0.5% was used and nuclei suspension was mixed with percoll solution (9:1) to remove myofibrils from the nuclei suspension (Hahn and Covault Analytical Biochemistry, 1990). Nuclei were tagmented and tagmented chromatin was used to make libraries using ATAC-Seq kit (Active Motif, 53150) and these were sequenced. ATAC-Seq kit from Active Motif (cat# 53150) ATAC-Seq: Indexed primers based on Illumina's Nextera adapters 