GSM7230024
Cells were sorted into ATAC-Seq Lysis buffer(10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% NP-40, 0.01% Digitonin) and then washed in a wash buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20). After, the cells were centrifuged at 800g and DNA tagementation could be performed using the transposition mixture (5 μl of Tagment DNA (TD) buffer, 3.2 μl PBS, 0.89 μl Tn5, 0.1% Tween-20, 0.01% Digitonin and 0.75 μl water) for 20 minutes at 37oC. Sequencing ready libaries were prepared by incorporating Illumina adaptors with PCR and then purifying and size selecting the DNA using AMPure beads