GSM8330402
Cells were fixed with 1% formaldehyde for 10min at room temperature. After quenching and cell lysis, chromatin was sonicated by using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared with size selection step using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7103S, NEB) complemented with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) (E7335S, NEB). 500ng DNA was used as starting material for input samples and 1.934-30.45ng DNA was used as starting material for ChIP samples. Libraries were amplified using 7-9 cycles for input samples and 12-14 cycles for ChIP samples on the thermocycler according to the protocol. Post amplification samples were beads purified and validated using Agilent High sensitivity DNA kit.