GSM7105733
Three to five 4-5 µm thin slices of formalin-fixed, paraffin-embedded tissue samples were deparaffinized using xylene and 96% ethanol. Total RNA was extracted using a commercial kit according to the manufacturer´s protocol: FFPE RNA/DNA Purification Plus Kit (Norgen Biotek, Thorold, Canada). The extracted RNA was eluted in 30 µl of RNase-free water. Two pools of extracted RNA from malignant and non-malignant samples were prepared for sequencing analysis. The RealSeq®-Biofluids, Plasma/Serum miRNA Library Kit for Illumina® sequencing (RealSeq Biosciences, Santa Cruz, CA, USA) was used for library preparation. The whole procedure was carried out according to the manufacturer's protocol. The input amount of RNA was 150-170 ng.