GSM7068183
The pelleted nuclei were incubated in 25 μl fresh TD buffer (10 mM Tris-HCl, pH 8.0, 5 mM MgCl2, and 10% dimethylformamide) with 2.5 μl Tn5 transposase for 30 min at 37℃. Purify DNA fragments using a QIAquick PCR purification kit (Qiagen, Cat. No. 28106). The library was amplified as described in the provided manual using the KAPA HiFi HotStart ReadyMix (Roche, KK2061). There was additional purification with a QIAquick PCR purification kit.