GSM7105854
The in situ Hi-C libraries was performed as previously described (Rao et al., 2014). Briefly, cells were grown to about 70%-80 % confluence, washed with PBS, fixed in 1% formaldehyde were suspended in Hi-C lysis buffer, to which 100 U MobI restriction enzyme (NEB, R0147) was added for overnight chromatin digestion. Free ends were labeled with biotin and then ligated together in situ. Crosslinks were reversed, the DNA was sheared to 300-500 bp and then biotinylated ligation junctions were recovered with streptavidin beads. Hi-C libraries were amplified using PCR and constructed according to the NEBnext library preparation protocol (NEB, E7335) and sequenced on the Illumina HiSeq X Ten platform.