GSM7156975
Genomic DNA was PCR amplified using KAPA HiFi HotStart ReadyMix (2X) (Roche). A second round of PCR with primers containing Nextera sequencing adapters enriched the target locus. Individual samples were indexed using xGenTM UDI IDT index primers (IDT). Indexed samples were pooled and sequenced on an Illumina Miseq and analyzed.