GSM7611251
T cells were isolated on day 7 post immunization using FACS sorting of CD45.1+ CD8+ cells. Cells from an individual condition (adjuvant, LM or VV) were stained with an unique oligo-tagged anti-CD45 “hashtag” antibody (Biolegend) and then combined into one sample. 20,000 hashtagged OT-I cells were loaded into Single Cell A chips (10x Genomics) and partitioned into Gel Bead In-Emulsions in a Chromium Controller (10x Genomics.) Gene expression and HTO libraries were prepared according to the protocols provided by the 10x Genomics Chromium Single Cell 3' Reagent Kits v2 User Guide by the CU Anchutz Genomics Core.