GSM7230760
The oocytes and embryos were collected as Smart-seq2 or Stacc-seq protocols in the paper, and the extraction was amplificated or used for the library directly as in the paper. For RNA-seq, RNA libraries for RNA-seq were prepared using a kit of Vazyme (TD502) and performed as the protocol of manufacture. For stacc-seq, Stacc-seq libraries performed according to the previous report.1 μl fully dissolved 5% digitonin  was added to Buffer1 (10 mM Tris-HCl pH = 7.4, 150 mM NaCl, 0.5 mM spermidine, 1 × EDTA-free Roche complete protease inhibitor). 0.5 μl PA/G-Tn5  and 0.5 μg TDP-43 or Pol II antibody was added to prepared Buffer1 and incubated at 4℃ for 30 min. Meanwhile, oocytes and embryos were treated gently with Tyrode's solution for removing the zona pellucida, then were put into 200ml low-binding tube contained 6ml prepared Buffer1 and incubate 4℃ for 10 min . Then 35 ml the prepared Buffer1, mixture of PA/G-Tn5, and the antibody and 12.5 ml TTBL were added for cleaving the chromatin around the binding sites and transposing with adaptors. Then the DNA were purified and PCR as the stacc-seq steps in previously reported