GSM8207502
Visium Spatial Gene Expression Solution was used to measure total mRNA in the fresh frozen tissue sections and locations where gene activity occurred (10x Genomics). The tissue sections were fixed, stained on Visium Spatial Tissue Optimization Slide, and permeabilized. Libraries were prepared according to the Visium Spatial Gene Expression Guide CG000239 (10x Genomics, Pleasanton, CA, USA). The tissue sections placed on Visium Spatial Gene Expression Slide were fixed and stained, and cellular mRNA was captured using primers at gene expression spots.