GSM7439050
KPC-Cdh11+/+, KPC-Cdh11+/- and Cdh11+/+ cohorts (n>=4) were euthanized at 4-5 months of age. Pancreases were excised and single cell suspensions were prepared using a collagenase digestion solution (3 mg/ml Collagenase I (ThermoFisher Scientific Catalog #17018029), 100 µg/mL DNase I (Roche Catalog #11284932001), Dispase II (Roche Catalog #4942078001) in RPMI1640 media supplemented with 10% FBS (ThermoFisher Scientific, Catalog #11875101) for 1 hour with shaking at 37°C.  Digests were filtered through a 100 µm cell strainer and depleted of red blood cells using ACK lysis buffer, per manufacturer guidelines (Gibco, Catalog #A1049201). Remaining cells were analyzed for viability and CD45 expression. CD45-/7-AAD-  (non-immune) and CD45+/7-AAD- (immune) cells populations were sorted via fluorescently activated cell sorting (FACS) and collected for scRNA-seq preparation. Both immune (CD45+) and non-immune (CD45-) cell populations were independently sequenced using a Chromium Single Cell 3' Reagent Kit and Chomium instrument (10X Genomice, Pleasanton, CA). Library preparation was performed according to manufacturer's protocol.