GSM6752835
The libraries were prepared using the 10x Chromium Next GEM Single Cell 3' Kit, v3.1 and associated components. Completed libraries were QC'd and quantified using a combination of Qubit dsDNA HS, Agilent 4200 TapeStation HS DNA1000 and Invitrogen Collibri Library Quantification qPCR assays. The libraries were pool in equimolar proportions and the pool quantified again using the Invitrogen Collibri qPCR assay. The pool was loaded onto two lanes of an Illumina NovaSeq 6000 SP flow cell (v1.5) and sequencing was performed in a custom paired end format, 28 cycles for read 1, 2 10 cycle index reads and 90 cycles for read 2. A v1.5, 100 cycle NovaSeq reagent cartridge was used for sequencing. The 28bp read 1 includes the 10x cell barcodes and UMIs, read 2 is the cDNA read. 