GSM6481238
Splenic single cell suspensions were obtained by mashing the spleens through a 70-µm cell strainer. Then stained with labelled antibodies and sort B cells for CD19. RNA was harvested using Rneasy mini plus kit (Qiagen). 2 ug of total RNA was used for the construction of sequencing libraries. Libraries were then constructed with the NEB Next Ultra II FS kit (E6177S, NEB) using the prime-seq specifications