GSM7998233
Total RNA was isolated using DirectZol RNA Miniprep kit (Zymo Research). 1 ug RNA per sample was used for library construction. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for directional library.