GSM8117800
The DNA were sheared into 200-300 bp by Bioruptor® Pico, 1% sample was saved as input and the rest samples were incubated overnight with antibody for each reaction in 4 °C. Dynabeads Protein G was added to each reaction the next day and incubated for 4 hours in 4 °C. The samples were washed sequentially with low salt wash buffer, high salt wash buffer, LiCl wash buffer and TE buffer. DNA was eluted from the beads and reverse-crosslinked in 0.2M NaCl at 65°C for 4 hours. The input DNA and ChIPed DNA were prepared for ChIPseq. ChIP-Seq libraries were prepared using NEBNext® Ultra™ II DNA Library Prep kit (NEB, E7103). Illuminal NextSeq 2000 P2 100cycles