GSM7689711
Tibial and femoral SSPCs were isolated by centrifugation. Bone marrow was resuspended in growth media (GM) [DMEM containing 10% FBS and 1% penicillin/streptomycin;  and then plated in 150cm2 tissue- culture flasks. Media changed the following day and then every two days. All cellular assays were performed with SSPCs at passage one from at least three different mice and young and middle-aged SSPCs subjected to ATAC-sequencing. Libraries were prepared for sequencing using standard Illumina protocols