GSM7438865
mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. The strand-specific cDNA sequencing libraries were generated using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, USA), and index codes were added. Samples were purified (AMPure XP system) and clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq 4000 PE Cluster Kit (Illumina, NEB, USA). After cluster generation, the stranded, poly-A selected libraries were sequenced on an Illumina NovaSeq 6000 platform by Novogene Bioinformation Technology.