GSM7689559
PRO-seq protocol was performed as in Serebreni, L. et al. Functionally distinct promoter classes initiate transcription via different mechanisms reflected in focused versus dispersed initiation patterns. EMBO J 42(10), e113519 (2023) with a single modification: the nuclear run-on was performed at 37 °C for 3 min. PRO-seq libraries were sequenced in  paired-end mode with 36-bp read length. To eliminate PCR duplicates, an 8-bp long unique molecular identifier (UMI) was incorporated at the 5′ end of the reads during the sample processing.