GSM6705066
Ultra-high molecular weight DNA (UHMW DNA) was extracted with the Nanobind CBB big DNA kit (Circulomics, #NB-900-001-01) following the manufacturer's protocol with minor modifications. We prepared an ultra-long nanopore sequencing library with a total of 35 µg of HMW DNA utilizing the Oxford Nanopore Technologies (ONT) sequencing library kit SQK-ULK001 following the manufacturer's protocol with minor modifications.