GSM7106564
Livers from between 20-30 larvae at  120 hpf were manually dissected and put in 500 ul of Trizol. RNA was extracted the same day of the liver dissection. Total RNA was treated by DNAseI (Thermo Scientific) for 30 minutes at 37C followed by RNA purification (RapidOut  DNA Removal Kit–Thermo Fisher Scientific). 80-120ng of total RNA was used for libarary prep. Samples were collected from pools of 5 independent clutches RNA quantitiy and quality was assesed by Qbit. 80-120ng of total RNA was used for libarary prep. RiboZero was  used to remove ribosomal RNA and the remaining sample  was used for library preparation according to manufacturer's  instructions (Illumina). Libraries were  sequenced on HiSeq 2500 (Illumina) to obtain 150 bp paired end  reads.