GSM6857315
MIN6 cells were washed and fixed in 1% formaldehyde for 10 min at room temperature. Crosslinking was quenched by adding glycine to a final concentration of 125 mM. Thereafter, ChIP assay was carried out using Magna ChIP HiSens Chromatin Immunoprecipitation system (EMD Millipore). Chromatin shearing was achieved by sonication (Bronson sonifier 250). Chromatin was immunoprecipitated either with control IgG (Cell Signaling) or anti-CtBP2 (Active Motif, 61261). The library was constructed with NEBNext UltraII DNA Library Prep Kit for Illumina.