GSM7957499
RNA was isolated using the miRNeasy micro Kit (Qiagen) and Covaris Cryo-Prep homogenization. To avoid contamination by genomic DNA samples were treated by on-column DNase digestion (RNase-Free DNase Set, Qiagen). Total RNA and library integrity were verified on LabChip Gx Touch 24 (Perkin Elmer). Approximately 100ng of total RNA was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Takara Bio). Sequencing was performed on the NextSeq500 instrument (Illumina) using v2 chemistry with 1x75bp single end setup or NextSeq2000 using P2 flowcell with v3 chemistry with 1x72bp single-end setup.