GSM6706763
Total RNA was extracted by TRIzol Reagent (Life Technologies, CA, USA) according to the manufacturer's instructions, and RIN values were measured using an Agilent Bioanalyzer 2100 (Agilent Technologies, CA, USA) to inspect RNA integrity. Qualified total RNA was further purified using an RNAClean XP Kit (Beckman Coulter, Inc. CA, USA) and RNase-Free DNase Set (QIAGEN, GmBH, Germany). RNA libraries were prepared for sequencing using a VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, Nanjing, China).