GSM7996592
Sorted IEMCs and LPMCs were lysed using lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3mM MgCl2, and 0.5% IGEPAL CA-630). Nuclei were pelleted by centrifugation (4 °C, 500g, 30 minutes) and resuspended in 50-μL reaction mix (Vazyme). DNA fragments were purified using a purification kit (QIAGEN, Hilden, Germany). The libraries of DNA fragments were amplified by PCR (Vazyme) and  purified using 0.5×/1.2× DNA clean beads (Vazyme). The quality of libraries was evaluated by Agilent 2100 Bioanalyzer.