GSM7998153
2× 107 BeWo were crosslinked with 1% formaldehyde (CST, #12606) diluted in PBS for 10 minutes at room temperature with rotation followed by 0.125 M Glycine treatment to quench the fixation. First, the cell nuclei were sequentially lysed, pelleted, and resuspended.2× 107 BeWo were crosslinked with 1% formaldehyde (CST, #12606) diluted in PBS for 10 minutes at room temperature with rotation followed by 0.125 M Glycine treatment to quench the fixation. First, the cell nuclei were sequentially lysed, pelleted, and resuspended ChIP-seq libraries were built using the KAPA DNA HyperPrep Kit (Roche, #KK8502) following the manufacturer's instructions.