GSM6577628
6 million cells were fixed with 1% formaldehyde for 10 minutes. After quenching with glycine and cell lysis, chromatin was sonicated using a Diagenode Twin Sonicator. Input was then taken from supernatant. IP fraction was pulled down using H3K36me3 antibody. A Drosophila spike-in chromatin was also prepared as a control. ChIP-seq libraries were prepared using the ThruPLEX DNA-seq kit.