GSM8423261
Total RNA was extracted using Trizol reagent (ThermoFisher, 15596018CN) following the manufacturer's procedure. The total RNA quantity and purity were analyzed by Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, 5067-1511), high-quality RNA samples with RIN number > 7.0 were used to construct the sequencing library.