GSM8328987
IMR90 were washed with 1x PBS and harvested directly in Trizol (Invitrogen, 15596018). RNA was isolated from this lysate by using the Direct-zol RNA MiniPrep Kit (Zymo Research, R2052:1074069) including an on-column DNase I treatment step according to the manufacturer's instructions. The library was constructed with the TruSeq kit (Illumina) before paired-end sequencing on a NovaSeq6000 platform (Illumina)