GSM7823228
Livers from 40 larvae / replicate were dissected and collected in the lysis buffer from ReliaPrepTM RNA Miniprep Systems (Promega, Z6110). RNA was isolated from the lysed tissue by following the manufacture's instruction. cDNA synthesis, library preparation and Illumina sequencing was performed according to the manufacturer's instructions using the TruSeq Stranded mRNA Preparation Kit for Illumina (Ilumina #20020594). Purified libraries were quantified using a Quibit fluorometer (Life Technologies) and the quality assessed on the Agilent Bioanalyzer (Agilent Technologies).