GSM8329246
Total RNA was extracted using Qiagen RNeasy Mini Kit from DC2 cells exposed to shear stress of 1 dyn/cm2 for 3 hours and DC2 cells kept in static conditions . The NEBNext Ultra II directional RNA library prep kit for Illumina (New Englands Biolabs Inc., Ipswich, MA, USA) was used to prepare total RNA sequencing libraries according to manufacturer's instruction using 1 ug of total RNA