RNAprep pure Cell kit (DP430, TIANGEN) were used for extracting cellular total RNA of PBLs, according to the manual. Fifty nanograms of total RNA were reverse transcribed into complementary DNA (cDNA) using a unique primer containing ATCAATC barcode and 16bp UMI sequence. Second-strand cDNA synthesis and whole transcript amplification were then implemented based on poly-A tagging. For conversion from amplified cDNA to sequence library DNA, 100 nanograms of amplified second-strand cDNA was fragmented into 400 bp sequences. After purification, fragmented cDNA was end-repaired and ligated with a truncated sequence adaptor, which had a pool barcode sequence. The resulting sequence library DNA was further enriched by PCR amplification. The library DNA was sequenced by paired-end strategy using a HiSeq X Ten sequencing system (Illumina). The sequence specifications were as follows: Read1, 151 cycles; Index1, 8 cycles; Read2, 151 cycles. It sequenced the ATCAATC barcode site and the UMI site at Read1, the pool barcode site at Index1, and the transcript sequence at Read2.