GSM8014247
Total RNA was extracted from cells using the Monarch Total RNA Miniprep Kit (T2010S, NEB) that includes treatment with DNase I for 15 minutes at 37°C to remove any DNA remnants. RNA quality was evaluated at the Bioanalyzer 2100 DNA Chip 7500 (Agilent Technologies). RNA-seq libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760L) for Illumina following the manufacturer's instructions. Samples were subjected to beads-purification (NEBNext Sample Purification Beads, E7767L), and the quality of the isolated libraries was evaluated at the Bioanalyzer 2100 DNA Chip 7500 (Agilent Technologies).