GSM7794270
After sorting, CD8+CCR4+ T-cells were stored in TRIzol Reagent (15596026, Life Technologies) at -80°C until RNA extraction was performed. Total RNA was extracted with Zymo-Spin IC Columns (C1004-50, Zymo Research) and Direct-zol RNA MiniPrep Kit (R2050, Zymo Research) according to manufacturer's instructions. NEBNext Ultra Directional RNA Library Prep for Illumina (New England BioLabs Inc.) and the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) kits were then used for library and cDNA synthesis and addition of barcode sequences, as per manufacturer's instruction.