GSM8253506
HA tagged ribosomes were immunoprecipitated from homogenized tissue using the anti-HA antibody ( Roche, Cat#11867423001), following the protocol described in Sanz et al., 2019. RNA was extracted following manufacturer's instructions in the RNAeasy Minikit (Qiagen). Library preparation and sequencing for ribosome associated mRNAs were performed by the UCSD IGM Genomics Center using Illumina Stranded mRNA Prep.