GSM8117413
Two weeks after adoptive transfer, WT (Osr2fl/fl OT-I CD45.1-CD45.2+) and Osr2 knockout (Osr2fl/fl Lck-Cre OT-I CD45.1+CD45.2+) T cells were flow-sorted for 10X single cell 3' V3 library preparation. Cells were counted and loaded onto the Chromium Controller (10 × Genomics) for a target recovery of 1 × 10^4 single cells. Samples were processed per the manufacturer's protocol and sequenced on an Illumina NovaSeq6000 sequencer using a 150-bp kit with paired-end reads.