GSM6751673
The cells were then crosslinked with fresh 1% formaldehyde (Thermo Fisher Scientific) for 10 min at room temperature (RT) and quenched with 125 mM glycine for 5 min at RT. Cells were washed three times in cold PBS containing protease inhibitors. Nuclei were isolated using the SimpleChIP Enzymatic Chromatin IP Kit (#91820, CST), and chromatin was sheared on ice using a Fisher sonic dismembrator with the setting of 10 cycles of 4 sec “on”