GSM7034680
Approximately 50,000 viable CD8+ T cells sorted from cocultures were used for each library preparation. Cells were lysed in 1 × Lysis Buffer and nuclei were isolated by centrifugation. A TruePrep™ DNA Library Prep Kit V2 for Illumina (Vazyme Biotech) was used to construct transposase-treated libraries. Qualified libraries were sequenced using an Illumina HiSeq X ten platform in pair-end 150 bp style.