GSM8207378
4 um AFA-fixed paraffin-embedded human needle kidney biopsies were used. 49 specific ROI were selected for spatial transcriptomic profiling usins DSP GeoMX Nanostring technology. Slides were incubated with GeoMx WTA (Nanostring) assay probe cocktail overnight. The slides were applied with morphology markers incubation before loading onto GeoMx machine. Library preparations was carried out according to the GeoMx NGS Readout Library Prep Manual (MAN-10117; NanoString). Libraries were sequenced on an Illumina NovaSeq6000 according to the manufacturer's instructions.