23-42K_s
Reprogramming of human, apparently healthy fibroblasts 8399 (Female, 19 yo), and 28808 (Male, 1 day old) was obtained by retroviral transduction of Oct3/4, Sox2, Klf-4, and c-Myc transcription factors as described in Li et al. (Mol. Ther., 2015: 23:1055).   Fibroblasts 942 (Female, 5 yo) were de-differentiated using Sendai Viral reprogramming vectors as described in Shanmughapriya et al (Nat Commun, 2018; 9: 3449). The fibroblasts cells 8399, 28808 and 942 are commercially available at Coriell (Camden, NJ, USA).  Reprogramming of human, apparently healthy lymphocytes SV20 (Male, 43 yo) was obtained using Sendai Viral reprogramming vectors as described in Shanmughapriya et al. (Nat Commun, 2018; 9: 3449). IPSC derived-cardiomyocytes were collected a week after the first transfection at approximately day 35 post-differentiation  IPSC-derived cardiomyocytes were maintened in RPMI medium supplemented  with B27  siRNA transfection was performed by using RNAiMax kit as per manufacturer's instruction RNA was extracted using RNAeasy kit (Qiagen) as per manufacturer's instructions Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina)