GSM7996471
Total RNA was extracted from each testicular sample using TRIzol reagent. RNA quality was assessed using Nanodrop-2000 and 1% agarose gel electrophoresis. Qualified RNA was used to prepare 1μg total RNA for cDNA library construction by cDNA-PCR sequencing kit. The cDNA library was sequenced on PromethION sequencer (Oxford Nanopore Technologies, Oxford, UK). These testing processes were conducted according to the protocol of the manufacturer.