GSM8423358
The C57BL/6 mice were hydrodynamic co-injected transposon-based vectors expressing Myr-AKT  and YAPS127A. 7 days later,mice were treated with or without NAV2729(NAV,30mg/kg) every other day for another 7days.aline-perfused mouse livers were sheared into small pieces with scissors and digested in a digest buffer (RPMI 1640 supplemented with 5% FBS, 0.05 mg/mL collagenase type I, 0.05 mg/mL collagenase type IV, 0.025 mg/mL hyaluronidase, and 0.01 mg/mL DNase I) at 37 °C for 30 min. After filtrating through 100 μm strainer, red blood cells (RBCs) were lysed with RBC lysis buffer (420301, BioLegend, San Diego, USA) for 5 min. The single-cell suspensions were blocked with purified rat anti-mouse CD16/CD32 (553140, BD Biosciences, San Jose, USA) for 30 min and incubated with the corresponding antibodies for 30 min at 4 °C.  The immune cells were isolated from 3 paired Myr-AKT/YAPS127A-driven iCCA by Fluorescence-activated cell sorting (FACS) according to expression of CD45 and sujected to scRNAseq. RNA libraries were generated by BD Rhapsody™ Express System  using standard Illumina protocols