GSM7822350
RNA was isolated by using TRIzol (Invitrogen) according to the manufacturer's instructions and quantified using a NanoDrop 3300 Fluorospectrometer. The RNA sequencing (RNA-seq) libraries were prepared from 1 μg of total RNA of PEO1 WT or PEO1-RFP. Libraries were generated using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs, Inc.). Library preparation was perform accoding to manufacter's protocol.