GSM7439120
Samples within each condition for each sex were randomly pooled in groups of three prior to RNA preparation. RNA was extractd using RNeasy Mini Kit (Qiagen). Isolated RNA was further purified and concentrated using the MinElute Cleanup Kit (Qiagen). RNA was quantified using the RiboGreen RNA Assay kit (Invitrogen) and assessed for quality using capillary electrophoresis (Agilent BioAnalyzer 2100; Agilent). Barcoded libraries were constructed for each sample using the TruSeq RNA v2 kit (Illumina). Libraries were size- (200 bp) selected and sequenced (50bp paired-end read, ~20 million reads/library) using Illumina HiSeq 2500.