GSM6751500
Treated INS-1E cells were washed twice with phosphate buffered saline (PBS) and  then collected in TRIzol reagent (Invitrogen, Carlsbad, USA). RNA was extracted by  first adding chloroform followed by centrifugation, to separate the organic and  aqueous components, and collection of the supernatant. An equal volume of ethanol  was added followed by centrifugation and collection of the supernatant. The aqueous  supernatant was then further purified using RNeasy Mini columns (Qiagen, Hilden,  Germany). Purified total RNA was eluted in RNase-free water. Total RNA samples were quantified and tested for quality using Agilent RNA 6000 Nano Kit. mRNA from the total RNA (0.1 ug) were converted into a library of template molecules suitable for subsequent cluster generation and sequencing using the Illumina TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA) using TruSeq RNA Sample Preparation v2 LS protocol and 12 indexed adapters.