GSM8117451
DNA was isolated using Quick-DNA Miniprep Plus kit (Zymo Research). 6.6ug of each samples were used for sRNA PCR amplification For preparation of the library containing single samples, we followed the manufacturer's instructions (Dharmacon). Purified DNA samples were validated and quantified by microfluidic analysis using the Bioanalyzer High Sensitivity DNA Assay kit (Agilent Technologies) and the 2100 Bioanalyzer with 2100 Expert Software. All samples were pooled with a 10% phiX spike-in. The library was sequenced using the Next Seq500 system performing single read (SR) runs covering at least 75 nt. (Illumina Inc., San Diego, CA, USA).