GSM7108019
Aortic tissues of each genotype and treatment arm were pooled together, cleaned, minced and digested in Hank's balanced salt solution containing elastase (0.5 mg/mL) and collagenase A  for 1 h at 37°C and then passed through a 40 μm filter. Single-cell suspensions were incubated with eBioscience 1X RBC Lysis Buffer for 3 min, collected in PBS containing 1% UltraPure BSA . Library was performed according to the manufacter's instructions(Chromium Next GEM Single Cell 3' Reagent Kits v3.1, 10X Genomics).