GSM8325596
RNA was extracted from cells using the RNeasy Mini Kit (Qiagen, 74106) using an on column DNaseI digestion step (Qiagen 79256). RNA was subjected to polyadenylated RNA enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module Kit (New England Biolabs, E7490L), and libraries were synthesized using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760L).