GSM7696022
RNA was then purified from cells using the PureLink™ RNA Mini Kit (ThermoFisher, 12183018A) and frozen (-80 °C). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using randomhexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library ordTTP for non-directional library.