GSM8030881
The BL-Hi-C experiments were performed as previous described (Dong et al, 2022; Liang et al., 2017) Cells were treated with 1% formaldehyde at RT for 10 min followed by quenching with 0.2 M glycine. Nuclei were isolated for subsequent experiments, including digestion with HaeIII (NEB), end-plus-A treatment, proximity ligation with biotin-labeled Bridge Linker, DNA purification and enrichment of biotin-labeled DNA with Dynabeads M-280 streptavidin. The enriched bead-bound DNA was subjected to end repair, adapter ligation, PCR amplification and DNA library construction, followed by sequencing on the Illumina NovaSeq platform (Annoroad Gene Technology Co., Ltd.).