GSM6576593
LNs were dissociated to single cells by enzymatic treatment (0.4 mg/mL Collagenase D (Roche), 0.6 mg/mL Dispase II (Roche), 40 μg/mL DNase I (Roche)) for about 30-45 minutes at 37ºC. Cells were pelleted an stained for stromal, hematopoietic and endothelial markers and sorted using FACS Aria III and Fusion. RNA libraries were prepared for sequencing using Chromium Single Cell 3' v3 protocol.