GSM6706602
RNA from HTR-8/SVneo cells was isolated by a phenol:chloroform extraction using TRIzol. Primary Trophoblast RNA isolation was performed with the Zymo Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following manufacturer's instructions after lysing cells in 350µL TRIzol. Novogene prepared libraries with high quality RNA after measuring the RNA Integrity Number (RIN)>7 with the Agilent High Sensitivity RNA Screentape Assay (Agilent Technologies; Santa Clara, CA). Sample library preparation was performed using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara Bio Inc.; Kusatsu, Japan) for primary syncytiotrophoblast cells followed by paired end sequencing (150BP) using an Illumina HiSeq with a read depth of 30 million read pairs/sample. Library preparation for HTR-8/SVneo cells was performed using an optimized NEBNext Ultra II kit followed by paired end sequencing (150BP) with a NovaSeq 6000 and a read depth of 30 million read pairs/sample.