GSM7439070
Total RNAs were extracted from HL-1 cells using TRIeasy™ Total RNA Extraction Reagent TRIeasy™ (YEASEN, China). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. 2 μg total RNAs were used for stranded RNA sequencing library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina (Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model. Raw reads containing more than 2-N bases were first discarded. Then adaptors and low-  quality bases were trimmed from raw sequencing reads using FASTX-Toolkit (Version  0.0.13). The short reads less than 16nt were also dropped. After that, clean reads were  aligned to the GRCm39 genome by HISAT2 allowing 4 mismatches. Uniquely mapped reads were used for gene reads number counting and FPKM calculation (fragments per kilobase of transcript per million fragments mapped.