GSM7822781
Maxilla were disassociated manually and then strained using a 100µm cell strainer. Tissues was further digested with collagenase type 1 (0.2mg/ml, Worthington Lakewood, NJ) for 1 hour at 37°C. Cells were washed, collected by centrifugation, and resuspended in incubation buffer (0.5% BSA in PBS). Viable CD45+ cells were isolated via flow cytometry as described above. The isolated cells were prepared in a single cell suspension and library preparation was performed using the Chromium Single Cell 3' Reagent Version 2 Kit (10X Genomics). Each sample was loaded and run on separate lanes. Droplets were subjected to reverse transcription and then cDNA amplified.