GSM6578164
Cells were lysed with Trizol (Thermo Fisher Scientific). Total RNA was then isolated using a Direct-zol RNA kit (Zymo Research) and quality validated on the Agilent 2200 TapeStation. RNA concentration was measured using a NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific). RNA libraries were prepared for sequencing using standard Illumina protocols.