GSM7439059
CSF and blood samples from two RRMS patients were sorted for IL-11R+ cells following RBC lysis for blood samples. In order to decrease batch-to-batch variations, all samples from each experiment were submitted for scRNAseq on the same day. Next-generation sequencing libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent kit v3 as per manufacturer's instructions. Library quality control was assessed using Agilent TapeStation for sizing (bp) and KAPA qPCR for concentration. Libraries were uniquely indexed using the Chromium i7 Sample Index Kit, pooled, and sequenced on the Illumina NovaSeq6000 sequencer in a paired-end, single indexing run.  Sequencing depth targeted 20000 mean reads per cell.