GSM8016111
Mediastinal LN were isolated at day 8 to prepare single-cell suspensions, and CD4+ T cells were isolated from single cell suspensions by negative separation using a mouse CD4+ T cell Enrichment kit (STEMCELL Technologies). ATAC-seq library purification was performed with the MinElute PCR Purification Kit (Zymo Research) and library size distribution was assessed using the Bioanalyzer High Sensitivity DNA Kit (Agilent). ATAC-seq libraries were quantified before pooling and sequencing using the real-time library quantification kit (KAPA Biosystems). Paired-end sequencing was performed on a S1 100 Cycle Flow cells v 1.5 with 100 cycles for each read.