GSM7439013
Kidneys from Dynabeads perfused mice were dissociated with Pronase/Collagenase supplemented medium for 20 minutes and the glomeruli isolated by magnetic field before crosslink with 1% methanol free formaldehyde. The reaction was quenched with 0.125M Glycerin and the glomeruli sonicated using a S220 focused-ultrasonicator (Covaris). MicroPlex Library Preparation Kit v2 (Diagenode, C05010013)