GSM6481130
Synovium were dissected then digested for 35 minutes at 37C in a volume of 1.5ml containing DMEM with 400 μg/mL collagenase IV, 400 μg/mL liberase, 400 μg/mL DNaseI. Samples were vortexed for 10 seconds at 0, 15 and 30 minutes of digestion. Cells were then pelleted by centrifugation at 500 x g for 5 min (4C) then resuspended in DMEM for counting and submission. Libraries were constructed according to the standard 10x Genomics Single Cell 3' protocol.