GSM7996439
all tissues were immediately frozen in liquid nitrogen and stored at -80°C. Tissue samples from biological replicates were pooled for RNA extraction at each sample point. Mycelia was grinded using a precooled mortar and pestle, homogenized with TRI REAGENT (Invitrogen), and then phase separated by adding chloroform. The nucleic acid phase was precipitated with isopropanol, washed with 75% ethanol, and solubilized in RNase-free water. RNA clean-up and DNase treatment was performed using Nucleo-Spin RNA (MACHEREY-NAGEL) and RNase-free DNase Set (QIAGEN), following the manufacturer procedures. RNA quantity and quality were analyzed using Nano-drop ND-1000 Poly-A selection and cDNA libraries preparation for RNA were prepared according to the Illumina mRNA Sample Preparation Guide Illumina RNA-seq strand specific first-strand