GSM7439745
The immunoprecipitated RNA was purified using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions. RNA quality and concentrations were accessed using Bioanalyzer RNA kit and samples with RIN values > 8.0 were selected for library preparation. RNA libraries for RNA-seq were prepared using NEBNext Low input RNA library Prep Kit for Illumina (NEB, E6420) following manufacturer's protocols.