GSM7230214
Slide preparation and probe hybridisation was performed as per Nanostrong GeoMx protocols. Areas of interest were defined by imaging slides with GeoMx DSP and then exposed to UV light to release probes. Oligonucleotides were then collected with a microcapillary and deposited into 96 well plates for sequencing. Sequencing libraries were generated by PCR from the photo-released indexing oligos and AOI-specific Illumina adapter sequences, and unique i5 and i7 sample indices were added. Each PCR used corresponding GeoMx seq code primers (Nanostring) and was performed as per manufacturer's instructions. No template controls included all kit components but no RNA.