GSM7230085
Five C57BL/6 mice were treated with anti-CD137 (10ug, clone 3H3) twice a week for 14 days. Ten C57BL/6 mice were treated with an Ig control (100ug, clone HRPN) also twice a week for 14 days. Mice were sacrificed and spleens were harvested for T cell sort. Teff cells were sorted as CD44hiPD1-, precursor exhausted T cells were sorted as CD44hiPD1+Slamf6+Tim3-, and exhausted T cells as CD44hiPD1+Slamf6-Tim3+. Single-cell libraries (5′ gene expression) were generated using the Chromium Controller Instrument and Chromium Next GEM Single Cell 5' Library & Gel Bead Kit v1.1 according to the manufacturer's protocol (10× Genomics) The libraries were sequenced twice on a NextSeq 550 (Illumina) in pair-end sequencing 28 bp (read1) × 91 bp (read2) and a single index 8 bp in length.