GSM7695883
RNA was harvested using Aurum total RNA kit (BioRad). 500 ng of total RNA was used for the construction of sequencing libraries. rRNAs first were removed by Illumina Ribo-Zero Plus rRNA depletion Kit f(Part# 20040526, Illumina) following the manufacturer's protocol, then the depleted RNA were used for library construction with NEBNext®Ultra™II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, catalog # E7760L).