GSM8290434
RNA was isolated from approximately 100,000 splenic DCs using NucleoSpin RNA XS Kit (Macherey-Nagel). Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation. Libraries were prepared with 10 ng of total RNA using a Bioanalyzer RIN score greater than 8.0. ds-cDNA preparation was done by the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol and sequenced on an Illumina NovaSeq X Plus using pared end reads extending 150 bases.