GSM7611277
Polyadenylated RNAs were captured and reverse transcribed with an Oligo(dT) primer The RNA-seq libraries were constructed according to a Smart-seq2 procedures. Briefly, mRNA was fragmented and reverse transcribed. The cDNA library was subject to end repair, poly(A)-tailing, adaptor ligation, and PCR amplification of 12–15 cycles for sequencing library construction.