GSM7438370
Genomic DNA was extracted using the DNeasy Plant Mini Kit (Qiagen) and sonicated as described previously (Li et al., 2016, DOI: 10.1093/nar) The sonicated DNA fragments were purified and ligated with methylated adapters from the TruSeq DNA Sample Preparation Kit (Illumina) at 16°C overnight. Less than 400 ng ligated product was used for bisulfite conversion using the Methyl- Code Kit (Invitrogen) according to the manufacturer's guidelines.