GSM6481281
Total RNA was extracted using the Total RNA Extractor (Trizol) kit (B511311, Sangon, China) according to the manufacturer's protocol A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. Next, miRNA sequencing libraries were constructed using TruSeq Small RNA Sample Prep Kits (RS-200-0024, Illumina, USA) following manufacturer's recommendations. The library quality was assessed on the Agilent Bioanalyzer 2100 system. mRNA sequencing libraries were constructed from six total RNA samples using VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® (NR601-02, Vazyme, China)