GSM8068296
Total RNA was normalized to 100 pico grams prior to oligo-dT capture and cDNA synthesis with Takara's SMART-Seq v4. The resulting cDNA was assessed on the Fragment Analyzer with the High Sense Large Fragment kit and quantified using a Life Technologies' Qubit 3.0 fluorometer. Libraries were generated using Illumina's Nextera XT DNA Library Prep kit. Medium depth sequencing (>30 million reads per sample) was performed with an Illumina HiSeq 2500 on a Rapid Run v2, 125 base pairs, Paired End run.