GSM7230177
ChIP was performed usign the ChIP-IT High Seneitivity kit (Active Motif 53040). Chromatin shearing to 200-500 bp fragments was performed by sonication. 30 µg of sheared chromatin was subjected to immunoprecipitations using  anti-FLAG M2 beads (Sigma-Aldrich M8823). Eluates were treated with Proteinase K. Libraries were constructed using the TruSeq ChIP Library Preparation Kit (Illumina) according to the maufacturer's instructions.