GSM8426215
Lung single-cell suspensions for Dropseq were generated from right lung lobes. Briefly, right lung lobes were removed and minced before undergoing enzymatic digestion in a mix of dispase, collagenase, elastase and DNase for 20-30 min at 37°C with agitation. Following straining of cell suspension through a 40 micron mesh filter, cells were centrifuged and counted in PBS with 10% FCS. For Dropseq, cell aliquots in PBS supplemented with 0.04% of bovine serum albumin were prepared with a cell density of 100 cells/µl. The library protocol was described in published paper in detail (https://doi.org/10.1038/s41467-020-17358-3)