GSM8031130
5μm thick FFPE slides were cut using a microtome. Deparaffin Protocol - Xylene 5min x3, 100% EtOH 5min x2, 95% EtOH 5min x1. 99°C ER2 (Ph9) 15mins. 37°C 1μg/mL Proteinase K 15mins. 10%NBF 5min, NBF Stop buffer 5min x2. 37°C. Label Protocol - WTA probe mix 1:10 18hr. 37°C 50%Formamide-2xSSC mix 25mins x2. Hybridisation Protocol - Syto13 200nM, CD3 532 1:40, CD34 1:100 + OPAL 620 1:200, PanCK 647 1:40 60mins. Scan Protocol - FITC - SYTO13 200ms, Cy3 - CD3 200ms, TxRed - CD34 100ms, Cy5 - PanCK 50ms Spatial transcriptome sequencing libraries were prepped with Nanostring Reagent kits according to manufacturer's suggestions.