GSM7957465
For analysis of gene expression changes in fibrocystic kidneys of postnatal mice, whole kidneys were isolated from wildtype and Hoxb7-Cep120 animals at P15. The kidneys were weighed and then grounded using pestle and mortar in liquid N2 bath and then lysed in Trizol, then total RNA was isolated using Direct-zolTM RNA MiniPrep Plus (Zymo Research). ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol