S03D_p
Cutaneous biopsies were taken from buttock skin, including unexposed skin and from skin exposed to a single low (physiological) dose of SSR, at 30 minutes, 3 hours and 24 hours post-exposure, in n=6 SU patients and n=6 HC. Total RNA was extracted from bisected 5mm skin punch biopsies at baseline, 30 minutes, 3 hours, and 24 hours post UVR provocation using the RNAeasy Plus Universal Minikit (Qiagen, Manchester, UK) according to manufacturer's protocol and guidelines. Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.