GSM8290098
Isolation of total cellular RNA from cultured hepatocytes or liver was pertranscription factors are expressed. formed using the RNeasy Kit (Qiagen) The quality of the RNA samples was analyzed using an Agilent RNA 6000 nano kit (Bioanalyzer, Agilent Technologies). Samples with RIN scores above 9.5 were used in library preparation. One microgram of total RNA was used to isolate mRNA (NEBNext Poly(A) mRNA magnetic isolation module). Libraries of the resulting mRNA were prepared using a NEBNext Ultra II RNA library preparation kit. All samples were sequenced on an Illumina NextSeq 2000. Three replicates were sequenced for each category (female: Normal, Mild, Moderate; male: Normal, Moderate, Severe).