GSM6856459
Individual frozen taste papillae were homogenized in RLT-DTT buffer (Qiagen) using a single 30 second, 6000 rpm cycle on a Precellys24 homogenizer (Bertin Corp., Rockville, MD). RNA extraction followed the recommended RNeasy Mini fibrous kit protocol (Qiagen, Germantown, MD). RNA quality and quantity were determined using the Agilent Bioanalyzer RNA6000 Chip (Agilent, Santa Clara, CA). Library construction and RNA sequencing was performed by Novogene Corporation Inc. (Sacramento, CA) using standard illumin protocols