GSM7035319
Total RNA was extracted using TRI reagent Total RNA was enriched for messenger RNA (mRNA) by oligo dT capture using the NuGEN Universal Plus mRNA-seq + UDI library kit (NuGEN 9144-96). Captured mRNAs were fragmented, reverse transcribed into cDNA and ligated with unique dual indexed (UDI) sequence adapters and PCR amplified to generate indexed libraries. Validation of library preparations was performed using the Agilent Bioanalyzer. Prior to pooling, quantification of the indexed libraries was performed using the Roche LightCycler96 RealTime PCR system.