GSM7070365
DNA was extracted using the Allprep DNA/RNA Mini Kit (Qiagen). 300ng of DNA was sonicated using the S220 Ultrasonicator (Covaris) and libraries were prepped using the Tecan Ovation Ultralow Methyl-Seq Kit (Tecan) with bisulfite conversion done with the Epitect Bisulfite Kit (Qiagen). Libraries were amplified using 11-13 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using Agencourt Beads. Libraries were validated using the Agilent DNA D1000 Screntape Assay Kit.