GSM8118570
Collected tissue samples or tissue slices were dissociated using the Adult Brain Dissociation kit (Miltenyi Biotec) on gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec) according to the manufacturer's instructions. Microwell 3' scRNA-seq were performed as described in Yuan et al. Sci Rep (2016). Briefly, individual single cell was compartmentalized with a barcoded-mRNA-capture-bead (MACOSKO-2011-10, ChemGenes) and lysed in microwell-based platform, mRNA were captured and reverse transcribed on the bead, and cDNA-beads were pooled for PCR amplification and illumina Nextera library construction for each sample.