GSM7962220
For ATACseq, cells were processed using the Omni-ATAC protocol. Sorted cells were washed once in 1X PBS, lysed, washed once in Wash Buffer and then the transposition reaction was carried out at 32°C for 30 minutes on a thermomixer set to 1000 rpm. Transposed chromatin was then purified using the Zymo Clean and Concentrator 5 Kit using manufacturer protocols. DNA was then run on PCR for 12 total cycles with dual-index barcoding primers. PCR reactions were then size-selected using AMPure XP beads and checked for quality and size distribution using TapeStation 4200 with D5000 reagents. Libraries were pooled at equimolar ratios for sequencing.