The total RNA of the prepared cells was extracted using an RNAprep Pure Cell Kit (DP430, TIANGEN) if the CSF total cell count (TCC) was over 100 × 106/L or an RNAprep Pure Micro Kit (DP420, TIANGEN) if the TCC was below 100 × 106/L, according to the manual. Fifty nanograms of total RNA were reverse transcribed into complementary DNA (cDNA) using a unique primer containing ATCAATC barcode and 16bp UMI sequence. Second-strand cDNA synthesis and whole transcript amplification were then implemented based on poly-A tagging. For conversion from amplified cDNA to sequence library DNA, 100 nanograms of amplified second-strand cDNA was fragmented into 400 bp sequences. After purification, fragmented cDNA was end-repaired and ligated with a truncated sequence adaptor, which had a pool barcode sequence. The resulting sequence library DNA was further enriched by PCR amplification. The library DNA was sequenced by paired-end strategy using a HiSeq X Ten sequencing system (Illumina). The sequence specifications were as follows: Read1, 151 cycles; Index1, 8 cycles; Read2, 151 cycles. It sequenced the ATCAATC barcode site and the UMI site at Read1, the pool barcode site at Index1, and the transcript sequence at Read2.