GSM7957354
Based on the structural characteristics of polyA tail in most mrnas of eukaryotes, the mRNA with polyA tail was enriched by Oligo(dT) magnetic beads The initial RNA for library construction was total RNA, total amount &gt; =1ug. Illumina's NEBNext® UltraTM was used to build the library RNA Library Prep Kit. After the library is constructed, initial quantification is performed using Qubit2.0 Fluorometer, dilution of the library to 1.5ng/ul, and then the insert size of the library is tested using Agilent 2100 bioanalyzer. After the library test is qualified, different libraries are carried out Illumina sequencing after pooling according to the requirements of effective concentration and target data volume, and then generated 150bp paired end reading. The basic principle of Sequencing is Sequencing by Synthesis.