GSM7823659
Total RNA was extracted from cultured control and HGS KD B16 cells, or the control and HGS KD B16 tumors harvested from tumor-bearing immunocompetent mice. The concentration of RNA was determined with Qubit® RNA Assay Kit in Qubit 2.0 Fluorometer (Life Technologies). RNA integrity was assessed with RNA Nano 6000 Assay Kit in the Bioanalyzer 2100 system (Agilent Technologies). 3 μg of RNA per sample were used to construct RNA-seq libraries. Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, cat#E7530L) and sequenced on an Illumina Hiseq platform, and 125/150 bp paired‐end reads were generated (Novogene Bioinformatics Technology). Four biological repeats for B16 cells and five biological repeats for B16 tumors were performed for the RNA‐Seq experiment.