GSM7439277
Total RNA of RAW264.7 cells was extracted using  a Quick-RNA Microprep Kit (ZYMO RESEARCH). Polyadenylated mRNA was separated from 200 ng of total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Sequencing libraries were generated using NEBNext Ultra 2 RNA LibraryPrep kit for Illumina (New England Biolabs) according to manufacturer's instructions. Library concentrations were assessed by a Bioanalyzer (Agilent) .