GSM6752839
Materials of HZ62 were collected and cross-linked using 1% formaldehyde (Sigma, F8775) and quenched with 0.2M glycine (Sigma, G7126) at room temperature. About 1g of samples were used for each library. After grinding into fine powder with liquid nitrogen, cell lysis by 1% SDS (Sigma, AM9822) at 4°C. The chromatin was fragment by sonication using a Bioruptor (Diagenode) into 200-600 bp. ChIP was performed using H3K4me3 and H3K27me3 antibody (Abclonal, A2357, A2363). Then, the protein-DNA complexes were reversed cross-linking using proteinase K (Invitrogen, AM2546) at 55°C. ChIP-DNA was extracted using phenol:chloroform:isoamyl alcohol (Sigma, P3803). ChIP-DNA libraries were constructed by using NEBNext Ultra II DNA library prep kit for Illumina (New England BioLabs, E7645) according to the manufacturer's guidelines.