GSM7689207
Embryos were dechorionated with a ~10 min incubation in 1 mg/mL Pronase, washed 3x in embryo medium, and dissociated on ice in 200 uL DPBS (without Ca2+ and Mg2+) with 0.1% BSA. Embryos were disrupted by pipetting 10x with a P200 pipette tip. 500 uL of DPBS + 0.1% BSA was added and cells were centrifuged at 300g for 1 min. Cell pellets were resuspended in 200 ul DPBS + 0.1% BSA and kept on ice. Prior to encapsulation, cells were passed through a 70 um cell strainer, and cell counts and viability were measured. After droplet encapsulation, barcoding, and library preparation using the 10X Genomics Chromium Single Cell 3' kit (version 3), data were sequenced on an Illumina NovaSeq 6000 sequencer. Droplet encapsulation, barcoding, and library preparation were performed per the manufacturer protocol using the 10X Genomics Chromium Single Cell 3' kit (version 3)