GSM7689281
Slide-seq spatial transcriptomics experiments were performed using the Curio Seeker Kit (Curio Bioscience) according to the manufacturer's instructions. Briefly, tissue sections from fresh-frozen murine ovaries were mounted on a 3mm x 3mm spatially indexed bead surface (Curio Seeker Platform, Curio Bioscience). After RNA hybridization and reverse transcription, the tissue section was digested, and the beads were removed from the glass tile and resuspended. Second-strand synthesis was then performed by semi-random priming followed by cDNA amplification. Sequencing libraries were then prepared using the Nextera XT DNA sample preparation kit. The libraries were pooled and sequenced on an Illumina NextSeq 2K (P3 flow cell) using the 100-cycle kit (Read 1 = 50 bp, Read 2 = 72 bp , Index 1 = 8 bp, and Index 2 = 8 bp).