GSM6706520
Peripheral blood samples were obtained from healthy adult volunteers. Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Lymphoprep (Axis-Shield, Oslo, Norway). CD4+ T-cells were isolated by magnetic-activated cell sorting (MACS) using the CD4+ T-cell Isolation Kit II (Miltenyi Biotec, Bergisch-Gladbach, Germany). Nuclei were isolated and ATAC-seq was performed according to published protocol (Buenrostro et al., Nature Methods, 2013) Library was constructed according to published protocol (Buenrostro et al., Nature Methods, 2013).