GSM6607126
On average, 5 um-thick cryostat sections were prepared for the extraction of total RNA from tumor cell-rich areas from pre- and post-tumor biopsy tissue after ROI selection based on H&E and CAIX markers, which were identified by a surgical pathologist (PK)  stained by H&E Each collection of oligo tags from one well (representing an ROI from the tissue section) was indexed with i7xi5 unique dual indexes using GeoMx SeqCode primers with 18 cycles of PCR.  After PCR, indexed AOIs were pooled and purified in two rounds of AMPure XP PCR purification using 1.2x bead:sample ratio.