GSM6706557
Total RNA extraction was performed from FFPE specimens using Maxwell RSC RNA FFPE Kit (Promega), following manufacturers' protocol. 500 ng of total RNA was used as starting material Libraries were prepared using QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015) with a UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen, 081), according to vendor's protocol