GSM8117436
Cells were lysed, DNA isolated by phenol/CHCl3/i-amyl alcohol.  DNA was sonicated with a Covaris E220 focued ultrasonicator to 200-400 bp size EBndG-DNA was isolated by biotinylation using Click reaaction Libraries were prepared with NEB Ultra IOI Library preparation kit.  3-8 ng DNA was used.  Libraries were amplified with 13 cycles on a thermocycle.  The libraries were sized selected from 250-450 using Agencourt AMPure XP beads from Beckman.  Libraries were validated using the Agilent 2100 Bioanalyzer.