GSM6576254
ChIP-Seq experiments were performed in duplicate for WT and MT cells (clones MT1 and MTs) using the High Sensitivity ChIP Kit (Abcam). 5x10e5 cells were used as input material for each immunoprecipitation.  Following crosslinking and cell lysis, DNA shearing was performed using Diagenode Bioruptor Sonicator at high power output, with 5 pulses of 30 seconds, followed by 30 seconds rest between each pulse. 1ug antibody was used for each reaction. Immunoprecipitated DNA was column purified and resuspended in 20ul DNA elution buffer. ChIP-Seq was performed on technical triplicates of WT and MT cells. All purified ChIP DNA was used as input for NEBNext Ultra II Library Prep Kits for Illumina.  Libraries were pooled and sequenced on a NextSeq 500 instrument (Illumina), with paired end 2 x 75 bp reads using High Output 150 cycle reagent kits.