GSM7231868
LCM was performed using the LMD 7000 system (Leica Microsystems Inc., Chicago, IL). FFPE human or mouse skin was processed onto a polyethylene naphthalate (PEN) slide designed for LCM processing (Leica Catalog #11505158). To acquire enough material for RNA sequencing of SGs, we isolated at least 1,000 SGs or 1,000,000 mm2 of tissue RNA was extracted from isolated SGs from mouse and human using a Qiagen All Prep DNA/RNA FFPE Kit (Qiagen Catalog #80234). Libraries were prepared using an Illumina Stranded Total RNA Prep with Ribo-Zero Plus Kit (Illumina Catalog # 20040529) with IDT for Illumina RNA UD Indexes, Set A (Illumina Catalog # 20040553).