GSM7439022
Kidneys from Dynabeads perfused mice were dissociated with Pronase/Collagenase supplemented medium for 20 minutes, the glomeruli isolated by magnetic field and further dissociated for 45 minutes with the same conditions. Single cells suspension was fixed with 1% methanol free formaldehyde, permabilized and stained with Podocin antibody before purification by FACS. Isolated podocytes were  Podocytes were then sonicated using a S220 focused-ultrasonicator (Covaris). MicroPlex Library Preparation Kit v2 (Diagenode, C05010013)