GSM7439099
Brains were collected for the dissection of VTA. Followed by total RNA extraction using the miRNEasy protocol, which was used for RNA-sequencing (paired-end) using the Illumina platform. cDNA fragments were 80bp to 500bp, with an average length of 250bp. RNA integrity numbers (RIN)for eachRNA sampleweredetermined using an AgilentTapestationandwereallabove 8.0. Illumina True-Seq Stranded libraries were prepared and quantitated by qPCR. Reads were 100nt in length. The RNAseq libraries were prepared with Illumina's 'TruSeq Stranded mRNAseq Sample Prep kit' (Illumina). Read 1 aligns to the ANTISENSE strand, whereas Read 2 aligns to the SENSE strand.  The libraries were quantitated by qPCR and sequenced on 11 lanes (8 lanes on one flowcell, 3 lanes on another flowcell) for 101 cycles from each end of the fragments on a HiSeq 4000 using a HiSeq 4000 sequencing kit version 1.  Adaptors have been trimmed from the 3'-end of the reads.