GSM7438954
Samples provided in a 96 well plate preserved in 5-10 uL of lysis buffer (1000-5000 cells per well). RNA-SPRI used for RNA isolation and purification. Modified protocol adapted for low yield non-nuclei samples from Picelli S, et al. (Nat Protocols, 2014) Samples received, RNA extracted and libraries made following adapted bulk RNA-seq protocol. Libraries barcoded for sequencing using Nextera i5 and i7 primers. Pooled at 4nM and sequenced in house using Illumina Next-seq 75cycle high throughput kit. R1- 38cycles, R2- 38, i1- 8, i2- 8. All QCs done by qubit/bioA.