GSM8200543
RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA-seq libraries were constructed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (E7760L). The NEBNext® Library Quant Kit for Illumina® (E7630L) and bioanalyzer were used to assess library quality and concentration then libraries were sequenced using the Illumina® NextSeq 500 and NextSeq 1000.