GSM7695730
Total RNA from isolated islets was prepared using RNeasy Micro Kit (Qiagen #74004) Sequencing libraries were prepared from 150ng total RNA using the TruSeq stranded mRNA library preparation kit (Cat# 20020595, Illumina Inc.) including polyA selection. Unique dual indexes (cat# 20022371, Illumina Inc.) were used. The library preparation was performed according to the manufacturers' protocol (#1000000040498)