GSM7823505
Cultured cells were harvested via trypsinization, followed by determination of cell viability. Avoidance of cell doublets was ensured with phase contrast microscopy. cDNA libraries were prepared according to manufacturer's instructions for the Chromium Single Cell 3' Reagent Kit v3. cDNA quantitation was done with HSDNA Chips on  Agilent Technologies' Bioanalyzer system (G2943CA) and amplified with the Chromium TM Single cell Library kit V3.