GSM6850214
tumor was harvested from NSG mouse mamary pad. Tumor tissue was dissociated with gentleMACS tissue dissociation kits (SUM149 xenograft tumors were disaggregated by gentleMACS kits (Miltenyi Biotec, #130-096-730), and incubated in TotelseqA hashtag antibodies (Biolegend, #394601, #394603, #394605, #394607, #394609, #394611, #394613, #394615) following the manufactural instructions. Live cells were isolated by EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, #17899). Single cell suspensions were processed according to 10xGenomics scRNAseq sample preparation protocol (Chromium Single Cell 3' v3.1 Reagent Kit, 10xGenomics). 1000 cells were targeted for each sample.) RNA libraries were prepared for sequensing using standard library construction protocol by 10x Genomics