GSM6753196
Total RNA was extracted using RNeasy kit (Qiagen) followed by on-column DNase I digestion according to the manufacturer's protocol. Genomic DNAs were purified using phenol–chloroform extraction and ethanol precipitation. Sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, Cat# 08098140702). ChIP assays were performed following the native ChIP protocol. ChIP assays were performed following the native ChIP protocol (Ohtani et al., 2018) with minor modifications. Total RNA-seq 786-O EV/KO Rep1 were sequenced 75 bp single end.  Total RNA-seq  769-P Day 0 Rep1 and Rep2 were sequenced 75bp paired-end. Total RNA-seq 786-O EV/KO Rep2 and other kidney cancer cell line total RNA-seq libraries were sequenced 50 bp paired-end. All ChIP-seq libraries were sequenced 50 bp paired-end.