GSM6856045
RNA was extracted from cell pellets washed with PBS using Directzol RNA MiniPrep (Zymo Research Corporation; 50-444-628). RNA yield and purity was tested using the Qubit 4 Fluorometer prior to analysis. Sequencing libraries were prepared using 250ng RNA using standard Illumina Tru-seq single indexing protocols and sequenced using the Illumina NextSeq 500 instrument.