GSM6752851
PDGFRβ-CreER::R26-TdT mice received transection SCI as described above. A 4-mm spinal cord segments centered at the lesion core were isolated and chopped into small pieces with sharp scissors. The pieces were digested with papain (Worthington Biochemical, LK003176) containing DNase (Worthington Biochemical, LK003170) for 1h at 37°C with slow rotation. Then add 1.0 mg/ml collagenase type II (Worthington Biochemical, LS004176) and 0.4 mg/ml neutral protease (Worthington Biochemical, LS02104) to incubate for 30 min at at 37°C with slow rotation. The cell pellets were resuspended with 25% percoll and centrifuged at 400 g for 10min to remove myelin debris. By using BD FACSAria Fusion cell sorter, cells were FACS sorted into DPBS buffer depending on tdtomato fluorescence. Library was constructed depending on  the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).