GSM6605640
After treatment, worms (now L4 larvae) were harvested and washed with M9 buffer three times to remove bacteria, before being resuspended in Trizol and flash frozen in liquid nitrogen. Total RNA was extracted by phase separation with chloroform, followed by the RNeasy Mini Kit (Qiagen) protocol. RNAse-free DNase was applied to RNA samples to remove DNA contamination. RNA was poly-A selected using oligo dT magnetic beads, fragmented and library prepared using TruSeq stranded kit (Illumina)