GSM7695889
Biopsied tissue underwent RNA extraction using Trizol reagent (Invitrogen). Biopsied tissue was manually homogenized in microcentrifuge tubes using a disposable micro-pestle (Fisher Scientific), and solubilized in 800 mcl Trizol. 200 mcl chloroform was added followed by centrifugation to separate  nucleic acids. The aqueous fraction was collected and RNA was precipatated by the addition of 500 mcl isopropanol and 1 mcl glycogen. Precipirate was pelleted by centrifugation, washed with 75% ethanol, and resolubilized in nuclease-free water. RNA-sequencing libraries were generated from 250 – 1000 ng of input RNA using the TruSeq Total Stranded RNA Library Kit (Illumina).