GSM8015115
Tissues were cut into 0.5mm3 pieces, and digested in HBSS containing 0.2% collagenase type II in 37°C with a shaking speed of 50rpm for about 40min. The digested suspension was subsequently filtered through a 40-µm cell strainer and washed with pre-warmed Dulbecco's Modified Eagle's Medium (DMEM)/F-12 50/50 supplemented with 10% fetal calf serum (FCS), 100U/ml penicillin, and 100U/ml streptomycin, and centrifuged at 300 x g for 5min. Library was performed according to the manufacturer's instructions  (single cell 3' v3 protocol, 10x Genomics).