GSM7439581
After the immunoprecipitation step, DNA was purified using AMPure XP beads. Briefly, beads were incubated with the reaction mix and washed several times using 70% Ethanol. Then, immunoprecipitated DNA was recovered by incubating  with 10 mM Tris, pH 8.5, 0.1 mM EDTA buffer and used for DNA library preparation Libraries were prepared from 10 ng of DNA using the NEBNext UltraTM II DNA Library Prep Kit for Illumina (New England Biolabs)