GSM7824337
Cells were crosslnked with 0.199 mj/cm2 UV-C light,  after nuclei isolation lysed with 1xNLB buffer, 1H4 or a rabbit polyclonal Ab against DHX9 was used to IP targets proteins, co-purifying RNA isolated with proteinase K digestion and purified with Oligo clean and concentrator-5 columns (Zymo). Custom library prep, described in Ilik et al., 2020. Isolated RNA is ligated to s-oligo on beads, reverse-transcribed, RNaseH digested, circularized with CircLigase and PCR amplified. FLASH-seq (Ilik et al., 2020)