GSM7070991
Cell pellets were resuspended in cold lysis buffer (10mM Tris-HCl pH 7.5, 10mM NaCl, 3mM MgCl2, 0.1% Igepal CA-630) and incubated on ice for 3 min. The tagmentation reactions were performed at 37°C and 1000 rpm for 30 min. The indexed samples were amplified using Nextera indexing primers and NEBNext High-Fidelity PCR Master Mix (NEB, M0541).