GSM6709941
Total RNA was extracted using RNeasy Plus Micro Kits (Qiagen, #74034). RNA was extracted from primary neonatal mice cardiac endothelial cells transfected with lentivirus by the GeneJET RNA Purification Kit (Thermo, K0732). Sequencing libraries were prepared using the TruSeq RNA Library Prep Kit V2(RS-122-2002, Illumina) according to manufacturer's instructions. All libraries were sequenced on the NextSeq500 sequencer (FC-404-2005, Illumina) using the 35nt paired-end sequencing protocol.