GSM8207000
For human samples, the whole blood was mixed with phosphate-buffered saline (PBS) and then gently put over on Ficoll-Paque (Cytiva) in a 50-ml tube. Buffy coat generated by centrifuging at 400g for 40 min at RT was collected. For single-cell purification from BAL, BAL was filtered with a 70-μm cell strainer (Falcon) and then centrifuged at 300g for 10 min at 4°C. Mouse BAL cells were obtained from BAL, briefly, alveolar lavages were pooled from BAL washes (PBS with 2 mM EDTA). Single cell suspensions were further passed through 70μm cell strainers once before the next step operation. 10X 5' Library & Gel Bead Kit v1.1 or 3' Library & Gel Bead Kit v3.1were performed for GEX library construction.