GSM8068097
Total RNA was purified using GenElute Single Cell RNA Purification Kit (Sigma-Aldrich RNB300), treated with DNase I on column (Sigma- DNASE70-1SET). RNA quality was checked using a BioAnalyser Nano 6000 Chip (Agilent). Libraries were prepared using SMART-Seq Stranded Kit (Takara Bio). The quality, size and concentration of cDNA libraries were checked using the High Sensitivity NGS kit Fragment Analyzer and qPCR (ROCHE Light Cycler 480).