GSM7439137
total RNA extraction was performed using RNeasy Plus Mini Kit (QIAGEN) according to manufacturer protocol. RNA integrity number (RIN) was determined using Agilent Bioanalyzer. All samples had RIN > 9.8. Library preparation was performed using NEBNext Ultra II Directional polyA mRNA Library Prep Kit. Sequencing was performed on Illumina NovaSeq 6000 with 60 million paired end reads per sample at 100 bp read length.