GSM6605623
P5 Nestin-EgfrF/F and control (EgfrF/+) pups were obtained from pregnant females. The morning when the litter appeared in the cage was considered P0.  The cortices and the hippocampal formation were dissected away from the ventral forebrain using microforceps in ice cold HBSS, and split into rostral and caudal halves. Samples were collected and Total RNA was extracted using the RNeasy Mini kit (Qiagen, catalog no. 74104). 700 ng of total RNA per sample underwent mRNA capture and was then fragmented at 94 °C for 6 min. Sequencing libraries were prepared according to the manufacturer's protocol using KAPA mRNA HyperPrep Kit (catalog no. KK8580). Indexes used: NEXTFLEX® Unique Dual Index Barcodes, catalog no. NOVA-512921. Next-generation sequencing was performed on a Novaseq 6000 (catalog no. 20012850) using NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles), catalog no. 20028401 for the 50x8x8x50 cycle configuration.