GSM7070418
RNA was harvested using Allprep DNA/RNA Mini Kit (Qiagen). 250ng of total RNA was used for the construction of sequencing libraries. RNA was rRNA depleted using NEBNext rRNA Depletion Kit (NEB). RNA libraries for RNA-seq were subsequently prepared using NEBNext Ultra Directional RNA Library Prep Kit with NEBNext Mulitplex Oligos for Illumina (NEB) following manufacturer's protocols.