GSM7696104
RNAs were extracted using the RNeasy Mini Plus kit from Qiagen. About 8ug was used to send to Novogene company for RNA-sequencing service. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.