GSM6709625
Plasmid libraries were isolated from yeast transformants using a modified Mini Prep (Qiagen) protocol. Briefly, sample pellets were resuspended in buffer P1, added to tubes containing ~100ul silica beads, and physically lysed using a bead beater. The lysate was transferred to a fresh tube and the remainder of the Mini Prep protocol was followed. PCR1 was performed with primers complementary to the reporter plasmid library and containing overhanging regions for multiplexing PCR. qPCR was used to determine the number of amplification cycles for PCR2. PCR2 was perfomed using unique dual indexes.