GSM6605782
Total RNA was extracted using the PureLink® RNA kit (Life Technologies) following their instructions and treated with RNase Inhibitor (Applied Biosystems™) and DNase I (Invitrogen™). RNA concentration and integrity were then determined using a NanoDrop system and an Agilent Bioanalyser, respectively. Libraries were generated using TruSeq Stranded mRNA (Illumina) kit and Poly(A) enrichment. Sequencing was performed using 100 bp single-end configuration (SE; 100 bp; 20 M).