GSM7439548
Colonic tumors were resected from animals that reached humane end points. Scraped tissues were flash frozen in liquid nitrogen and stored at -80ºC. Frozen samples were powderized in liquid nitrogen with a tissue homogenizer. RNA was extracted using the RNeasy Plus Universal kit from Qiagen, following the manufacturer's instructions. RNA concentration and quality were evaluated in Agilent Bioanalyzer/TapeStation. 1µg of RNA was used for RNA-Seq library preparation using Truseq Strand Specific Large Insert RNA Sequencing Unaligned v1. Library construction was performed by the Genomics platform team at the Broad Institute.