GSM7156898
Myeloid progenitors (lineage-negative c-kit+ IL7Ra-) were sorted using a BD FACSAria III (BD Biosciences) cell sorter as described in Rosu et al.(DOI: 10.1084/jem.20200662 ) and were resuspended in Ca and Mg free PBS containing 0.4mg/ml of UltraPure™ BSA (Thermofisher, AM2616).In total, 16,000 sorted cells were submitted to the 10X Genomics pipeline for encapsulation aiming for a recovery of 10,000 sequenced single cells as described in the manufacturer's instructions. Sequencing libraries were prepared using Chromium Single Cell 3' Reagent Kit V3 (10X Genomics) as per manufacturer's instructions and cDNA quality and quantity were controlled using Agilent High Sensitivity DNA Kit (Agilent) on a 2100 Bioanalyzer (Agilent).