GSM7155328
The quality and concentration of the single cell preparations were evaluated using an haemocytometer in a Leica DM IL LED microscope and adjusted to 400 cells/µl. 17,200 cells per sample were loaded in to the 10X Chromium controller and library preparation was performed according to the manufacturer's indications (Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 protocol). The resulting libraries were sequenced in an Illumina NovaSeq sequencer according to 10X Genomics recommendations to a depth of around 40,000 reads per cell.