GSM6605785
P0.5 backskin was treated with dispase, then the epidermis is separated from the dermis and further trysinized to get single cell suspendsion for ChIPseq. For MOF ChIPseq, cells were fixed and DNA fragmented, then pulled down using MOF antibody. For others, cells were subjected to Cut&Run based protocol for genomic DNA pull down. Pull-downed DNA were constructed into library using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®.