GSM6751843
SMART-Seq v4 Ultra Low Input Kit for Sequencing was used for full-length cDNA synthesis and  amplification (Clontech, Mountain View, CA), and Illumina Nextera XT library was used for sequencing  library preparation. Briefly, cDNA was fragmented, and adaptor was added using Transposase, followed  by limited-cycle PCR to enrich and add index to the cDNA fragments. The final library was assessed with  Agilent TapeStation.