GSM7067549
500 embryonic intestinal epithelial cells were isolated by FACS directly in 7ml of lysis buffer (Clontech) supplemented with 5% RNase inhibitor and stored at -80°C. PolyA+ mRNA was used for cDNA synthesis using SMARTer v4.0 kit (Clontech) according to the manufacturer's instructions. Amplification was performed for 15 cycles. After cDNA fragmentation (Covaris), libraries were prepared using Ovation Ultralow Library System (NuGEN) according to the manufacturer's instructions.