GSM8116367
The total RNA of leaves was extracted using the RNAprep Pure Polysaccharide Polyphenol Plant Total RNA Extraction Kit (QIAGEN, Germany).2 ug of total RNA was used for the construction sequencing library. Sequencing libraries were generated using NEB Next® Multiplex Small RNA Library Prep Set for Illumina® (NEB E7300L). Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment.