GSM6706625
TRIzol RNA isolation was performed on three biological replicates of IPs from YFP, TDPWT YFP, and TDPG298S YFP from mushroom bodies. RNA was quantified by nanodrop to bring it within range for ribogreen quantification of 0.15625 and 10 ng/ul. RNA was also checked using Agilent Tapestation High Sensitivitiy RNA screentape. 1 ng total RNA was used for SMART Seq HT PLUS (Takara Bio USA, Inc. Cat #R400748) following manufacturer's protocol. Determination of cDNA quality and quantity was determined via Agilent Tapestation High Sensitivity D5000 Screentape and Qubit dsDNA HS assay for input into library amplification. Libraries were quantified by Agilent Tapestaion High Sensitivity D1000 Screentape and Kapa Library Quantification kit for Illumina platforms. Libraries were pooled equimolarly, pools were quantified by Agilent Tapestation High Sensitivity D1000 Screentape and Kapa Library Quantification kit and loaded on the NovaSeq 6000 S4 flowcell and sequenced to 101 x 11 x 11 x 101 cycles.