GSM7035138
Specimens were collected in either HBSS + 10% FBS, spun down, then digested using Accutase, 1 mM EDTA, DNAse I, and papain. Cells were filtered through a 70 μm strainer, pelleted and washed, and then treated with an erythrocyte lysis buffer and washed again. The cells were then resuspended in PBS with 0.1% bovine serum albumin and 0.2% anti-clumping agent (Invitrogen) and immediately processed for scRNA-seq. Note that cells from three Eed-Het animals were pooled for a single 10x Chromium run. Samples were processed for scRNA-seq using Chromium Single Cell 3′ Paired-End, Dual-Index Library & Gel Bead Kit v3.1 (10X Genomics) targeting 10000 cells, then sequenced using NovaSeq 50bp (Illumina) flow cells.