Sample_21_s
At least 50 seedlings were pooled from three independent petri dishes for RNA extraction. Arabidopsis thaliana seeds were surfaced sterilized and sown in petri dishes with agarified (0.8% Bacto Agar) 0.5x MS medium, containing 1% sucrose and 0.05% MES at pH 5.7. After stratification for 3 days at 4°C, seedlings were grown in a CU-22L growth chamber (Percival) under 21°C and a 12/12 h (day/night) light cycle.  For treatment, 7 days-old seedlings were subjected to changes in temperature only during the light period. Long-term heat consisted of an entrainment treatment of 5 heat cycles (day 37C, night 21C, 12/12 h; age at harvest 12 days). Controls were kept at 21C (age at harvest 12 days). Total RNA was isolated from biologically duplicated samples using the the Plant-RNAeasy kit (Invitrogen), following provider's instructions. Libraries were prepared with the corresponding Illumina library preparation kit, following provider's instructions.