GSM7962413
Spleen were mechanically minced and dissolved in PBS containing 4 mM EDTA. Minced tissue was filtered through a 70 µm cell strainer. After centrifugation 300 g, 5 min. RBC lysis was performed using the RBC lysis buffer (Biolegend, Cat. 420302) for 4 min on ice. Cells were washed once with PBS containing 4 mM EDTA. Spleen single-cell suspensions were multiplexed and then used for GEM generation following the 10X Genomics protocol (CG000388 Demonstrated Protocol Chromium Next GEM Single Cell 3ʹ v3.1 (Dual Index) with Feature Barcode technology for Cell Multiplexing Rev B) targeting a cell recovery rate of 10,000 cells. GEM generation and library preparation were performed according to the 10X Genomics protocol (CG000388).