GSM7231117
RNA was extracted byTRizol following the product manual. For small RNA, total RNA was run using 15% TBE-urea gel, followed by cutting the 15-35 nt RNA fragments. RNA was extracted by incubating overnight in RNA gel extraction buffer (300 mM sodium acetate ( pH 5.5), 1.0 mM EDTA and 0.25% (wt/vol) SDS) Library was constructed by standard Illumina protocol. Small RNA-seq library was generated by NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7300S, New England BioLabs).