This part, including reverse transcription and whole-transcript amplification, was carried out in accordance with the protocols for the QUARTZ-SEQ2. 100 nanograms of amplified second-strand cDNA was fragmented into 400 bp sequences. After purification, fragmented cDNA was end-repaired and ligated with truncated sequence adaptors. The library was further amplified and purified under the conditions described in the QUARTZ-SEQ2 protocols. The library DNA was sequenced using a HiSeq X Ten sequencing system. The sequence specifications were as follows: Read1, 151 cycles; Index1, 8 cycles; Read2, 151 cycles.