GSM7996361
U2OS cells were seeded in 6 cm plates and treated with Doxycycline (500 ng/ml) for 1 or 4 days. 50,000 viable cells were collected by centrifugation at 500g for 10 min at 4 °C.  Cell pellets were lysed in 50 ul RSB Buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20 and 0.01% Digitonin) on ice for 3 mins.  Then cells were washed with 1 ml wash buffer (0.1% Tween-20, 10 mM Tris-HCl pH7.4, 10 mM NaCl, 3 mM MgCl2). Nuclei were collected by centrifugation at 500g for 10 min at 4°C. The pellet was resuspended in a 50 μl transposes mix (Vazyme TD501) incubated at 37 °C for 30 min in a PCR machine, and further cleaned by a Zymo DNA purification kit. ATAC-seq libraries were prepared using the library preparing kit (Vazyme, TD501). Transposed DNA was amplified for 10 cycles, and the library DNA was further size selected with VAHTSTM DNA Clean Beads (Vazyme, N411) to the range of 150bp - 700bp./