GSM7780649
After two washes approximately 100,000 PBMCs per sample were processed according to the 10X Genomics protocol. A single cell suspension was prepared and loaded onto the 10x Chromium Controller (10X Genomics) according to the manufacturer's protocol, with a target capture of 5,000 cells per channel. Sequencing libraries were generated using the NextGEM Single Cell 5' v1.1 kit (10x Genomics).