GSM8290445
For BRG1 CUT&TAG assays, 100,000 pre-adipocytes were harvest by trysin digestion and counted, CUT&TAG assays were conducted following the manufacturer's instructions (TD903, Vazyme). For ATAC-seq assays, 50,000 pre-adipocytes were harvest by trysin digestion and counted, and then nucleus were extracted, ATAC-seq assays were conducted following the manufacturer's instructions (N248, Novoprotein). BRG1 CUT&TAG libraries and ATAC-seq libraries were constructed  with extracted DNA fragments mentioned above following the manufacturer's instructions (TD202, Vazyme for BRG1 CUT&TAG or N239, novoprotein for ATAC-seq) respectively. For ATAC-seq libraries, additional fragment selection was conducted with 0.4×/0.8× beads ratio after DNA extraction.