GSM7689193
RPMI-1640, 10% ES-qualified foetal bovine serum, 2mM L-Glutamine, 0.1mM β-mercaptoethanol, 100IU/mL pen/strep, 1µM β-estradiol, 5% filtered cell culture supernatant from a Flt3L-producing B16 melanoma cell line cells were fixed with 1% formaldehyde for 10min. After quenching and cell lysis, the chromatin was sheared using a Bioruptor Pico sonicator (Diagenode)