GSM7070546
RNA was harvested using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer's protocol.A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina(NEB #7530,New England Biolabs, Ipswich, MA, USA).