GSM8068168
Total RNA was extracted using the hot phenol. RNA quality and quantity were assessed using a nanodrop (Thermo Scientific, USA). All library construction were prepared according to the NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina® (New England BioLabs Inc., Massachusetts, USA). Final libraries quantity was assessed by Qubit 2.0 (ThermoFisher, Massachusetts, USA) and quality was assessed by TapeStation HSD1000 ScreenTape (Agilent Technologies Inc., California, USA).