GSM7997909
Cells were partitioned into Gel Bead‑In‑Emulsions with a Target Cell Recovery of 5.000 total cells.Cell number and viabilitywere verified using a TC20™ Automated Cell Counter (Bio-Rad Laboratories, Hercules, CA, USA). cDNA sequencing libraries were prepared using the NextGEM Single-cell 3' mRNA kit (V3.1; 10X Genomics) following manufacturer's instructions. Briefly, after GEM-RT clean up, cDNA was amplified during 13 cycles and cDNA QC and quantification were performed on anAgilent Bioanalyzer High Sensitivity chip (Agilent Technologies). cDNA libraries were indexed by PCR using thePN-220103 Chromiumi7 Sample Index Plate.Size distribution and concentration of 3'cDNAlibraries were verified on an Agilent Bioanalyzer High Sensitivity chip(Agilent Technologies). Finally, sequencing ofcDNA libraries was carried out on a Illumina HiSeq 2500 (Illumina) to obtain approximately25,000-50,000paired-end 75bpreads per cell, respectively.