GSM7996715
Using control and Acly cKO mice at 5 weeks of age (n=3 Acly cKO/2 controls), RNA was purified from sciatic nerves using RNA Clean & Concentrator-5 (#R1013) 1000 ng total RNA was sent to Novogene for library preparation after PolyA selection and Illumina sequencing (Novaseq 6000). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis.