GSM8253701
Blood and tissue neutrophils from 10-12 week-old male mice  were FACS sorted using CD45, CD11b, Ly6G and DAPI labeling, with typical purities > 95%. Total RNA was prepared with the RNA Extraction RNeasy Plus Mini-kit (QIAGEN) cDNA amplification from neutrophil RNA and generation of index-tagged sequencing libraries were carried out using the Ovation Single Cell RNA-Seq System (NuGEN Technologies, San Carlos CA). Libraries were quantified using the dsDNA HS assay with the Qubit fluorometer (Life Technologies, Carlsbad, California). Average library size and size distribution were determined using a High Sensitivity DNA assay in an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara CA). Libraries were normalized to 10nM using Tris-Cl 10mM, pH8.5 with 0.1% Tween 20.