GSM7997862
A549 cells were cross-linked with 1% formaldehyde for 10 minutes at room temperature. The cross-linking reaction was subsequently quenched with 0.125 mol/L glycine. Cells were then resuspended in SDS buffer. The resulting cell pellets were resuspended in ice-cold immunoprecipitation (IP) buffer, and then subjected to sonication. The supernatant post-centrifugation was incubated overnight at 4°C with H2AK119ub antibodies. Protein A/G beads were then added at 4℃ for 4 hours. After thorough washes, samples were incubated at 65°C for 6 hours, and DNA was extracted. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit complemented with NEXTflex DNA Barcodes from Bioo Scientific.