GSM6576183
Total RNA was extracted with TrizolTM X-100 (Thermo) treatment and a total amount of 2 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using VAHTS mRNA-seq v2 Library Prep Kit (Illumina). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads and cut into small fragments for cDNA synthesis. After adenylation of 3' ends of DNA fragments, adaptor with hairpin loop structure were ligated, and then PCR was performed. High-throughput RNA sequencing were carried out using Illumina NovaSeq platform