GSM8015029
Tagmentation was performed for 1 hr in Dig-300 buffer and then fragmentated with 300µL tagmentation buffer incubated for 1 hr at TM. Extracted nucleic acid with phenolic chloroform and dissolved in 1xTE solution. The nucleic acid samples suspension was amplificated 13 cycles with 50µL amplification mix (TAE, TAB and P5, P7 primer) (TD901, Vazyme), and purified using VAHTS DNA Clean Beads (N411, Vazyme)