GSM7885390
Primary tumor: Tissue was minced using scissors and treated with enzymatic digestion solution containing 3 mg ml-1 collagenase A and 70 unit ml-1 DNase inDMEM 20 min at 37oC. Spleens: spleens were crushed and treated with red blood cell lysis buffer for 5 minutes on ice. NK cells were isolated using Miltenyi mouse NK isolation kit.  For both spleens and tumors, NK cells were further purified via FACS. sCAFs were isolated using magentic seperation (CD45-Epcam-) and further purified via FACS 3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science