GSM8067655
RNA was released from fixed and stained islet cells by treatment with resuspension buffer and Proteinase K from the Ambion Recoverall kit at 50˚C for 3 hours.  Depending on cell number, RNA was purified and DNA removed on a Recoverall column or RNA-Clean XP beads. Libraries were constructed using with the the NUGen Ovation FFPE or the Clontech SMARTER Pico kits.