title: ="RNA-Seq of juvenile grouse_"&B33
design: DNA was extracted from grouse feces using the DNeasy 96 PowerSoil Pro QIAcube HT kit and the fourth variable region (V4) of the 16S rRNA gene (252254bp) was amplified using 5 L of DNA extract and primers 515FB and 806RB. SparQ PureMag Beads were used to clean libraries and fragment sizes were verified using a LabChip GX instrument prior to sequencing on an Illumina MiSeq platform (v2 chemistry, 250-bp paired-end reads, 500 cycles). Sequencing data from MiSeq platform was de-multiplexed and converted to Fastq format using Illuminas bcl2fastq software.