title: Faecal microbiome diversity in long-term care residents during an antimicrobial stewardship programme and its association with multidrug-resistant bacterial colonization
design: Bacterial 16S rRNA 154 amplification and library construction was performed according to the 16S Metagenomic Sequencing Library Preparation Guide by Illumina. Briefly, 2.5 l of total DNA per sample was amplified using primers targeting the 16S rRNA V3 and V4 regions (Illumina, SD, USA), 22, 23 which provide information used for the taxonomic classification of microbial communities. Pooled V3-V4 amplicon libraries were sequenced using the Illumina MiSeq platform with a V3 reagent kit (Illumina) and paired-end 300-bp reads.