title: Gorlin_scRNA_r1
design: Tumors were dissociated by first mincing into pieces smaller than 1 mm in diameter and then incubated in 40 mL of a 0.5% collagenase solution (Gibco; 25200056) for 2hrs with rotation at 37°C . 5 mL of 0.25% Trypsin was then added and incubated for an additional 15min with rotation at 37°C . Cells were then FACS sorted for live cells, and then processed using the 10X Genomics Next GEM scRNA-Seq solution. Library construction followed the 10X Genomics Next GEM scRNA-Seq pipeline.