title: Metagenome sample of Folsomia candida
design: The concentration of total RNA was determined using the Qubit RNA Assay Kit (Life Technologies). 1ug RNA from each sample was used for rRNA removal by using Ribo-Zero Plus rRNA Depletion Kit The libraries were prepared using Nextera DNA Flex library preparation kit (Illumina) following the manufacturer's user guide. The initial concentration of DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). 50 ng DNA was used to prepare the libraries. The samples underwent the simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle PCR in which unique indices were added to the sample. Following the library preparation, the final concentration of the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were then pooled in equimolar ratios of 0.6nM, and sequenced paired end for 500 cycles using the NovaSeq 6000 system (Illumina).