title: rDNA of bacteria: bacterial community in a batch anaerobic digestion reactor without substrates at final point (PT_16S)
design: Biomass sample used for NGS was collected from a batch anaerobic digestion reactor (i.e., without substrates - positive control set) at the end of experiment (30 day). The biomass was used for the genomic DNA extraction. The obtained genomic DNA was used as template for high throughput sequencing. A universal bacteria primer set (i.e., 341F and 805R) was used to construct the library.