title: Amplicon-seq of rice PDS gene: embryogenic callus (Replicate 1     37C_Mb3Cas12a     Event C08-3)
design: The library consists of different amplicons (OsPDS gene and potential off-target sites) that were indivually amplified using specific primers tailed with Illumina sequencing primers.  Equimolar quantities of each PCR amplicon were pooled together and barcoded using iTru5 and iTru7 primers.