title: RNA-seq of tomato treated with microalgae Micractinium MACC360
design: n equal amount of RNA was added from each of the replicates to make a single pooled sample for each treatment.  For each sample, 1 g of total RNA was reverse-transcribed for 60 min at 42 C and for 10 min at 75 C in a 20 L reaction volume using RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) according to the manufacturers instructions. cDNA products were diluted 1:10 in RNAse-free water (Lonza, Verviers, Belgium).