title: RNA-Seq of Physcomitrium patens: ppscr-Replicate 2
design: Library preparation was performed at Nucleome Informaticsfacility.Total RNA (3 g) was used for generation of sequencing libraries using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs), following which index codes were assigned to each sample. The library quality was assessed using a Bioanalyzer 2100 system (Agilent Technologies). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using a TruSeq PE Cluster Kit v3-cBot-HS (Illumina). After cluster generation, the library preparations were sequenced on an Illumina Hiseq platform and 150-bp paired-end reads were generated