title: RNA-seq of rice: leaves
design: 1. mRNA purification and interruption; 2. Synthesizing the first strand of cDNA; 3. Synthesizing the second strand of cDNA and purifying the product; 4. End repair and 3 'end plus A; 5. Connection Adaptor; 6. Product purification and fragment selection; 7. PCR amplification; 8. PCR product purification; 31. Library quality inspection.