title: TLO_RF_Inner_Rep1
design: The tlo null mutant CC16  was lawned on RPMI solid media and cultured statically for 48hrs at 37C in the presence of a fluconaozle Etest strip. Cells were harvested from the zone of inhibition surrounding the ETest by washing with sterile PBS and RNA was then extracted using the RNeasy extraction kit (QIAGEN) as described previously by Fletcher et al., PMC10721199