title: U87-MG untreated rep1
design: U87-MG and DK-MG cell lines were used as references for cells with TERTp C228T and C250T mutations, respectively. The cell lines were mixed, subject to STAR-FACS and sorted for Alexa-647 signal (C228T mutation) or Cy3 signal (C250T mutation). After that the cells were either subject to RNAseq, scRNAseq or CUT&Tag protocols. For RNAseq, two fixation strengths were tested (1% PFA and 0.25% PFA). Primary GBM cell lines derived from distinct regions of the same tumor (OS23_2, OS23_3, OS23_4, OS23_5; all TERTp WT) were co-cultured with TERTp mutant primary GBM cell lines (OS25_4 or OS11_5) for 10-14 days and subject to STAR-FACS to separate TERp WT and TERTp mutant cells. The last co-culture experiment was perfromed with two primary GBM cell lines derived from the same tumor (OS21_3 - TERTp WT and OS21_8 TERTp MUT). After 14 days of culture, the cells were subject ot STAR-FACS to eseparate WT and MUT cells and subject to RNAseq.