title: Acanthogobius hasta target enrichment sequences from environmental DNA
design: Targeted sequences of Acanthogobius hasta were enriched from eDNA. We sheared the 1000 ng purified DNA into around 250 bp using a Covaris M220 Focused-ultrasonicator (Woburn, Massachusetts, U.S.A.). Sheared DNA was used to prepare libraries with two different indexes incorporated in both ends. All indexed libraries were mixed into one tube equimolarity for gene enrichment and Illumina sequencing on a HiSeq 2500 sequencer (Illumina, Inc, San Diego, CA, U.S.A.).