title: RNA-Seq of C. crescentus NA1000: discr mutant, replicate 1
design: The strains C. crescentus NA1000 and iscR were grown in PYE medium up to OD600nm = 0.5 at 30C with agitation at 200 rpm. Aliquots from three independent biological replicates of the wt strain and three biological replicates of iscR were collected. Total RNA was extracted from 10 ml-aliquot cultures using the RNeasy Mini kit (Qiagen) and RNA concentration and integrity were checked Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbroon, Germany). rRNA depletion and cDNA libraries were prepared using the Illumina Stranded Total RNA Ligation Kit with RiboZero Plus (Illumina) and subsequently sequenced using the NextSeq 500/550 Mid Output Kit v2.0 (150 cycles) (Illumina) on an Illumina NextSeq 500 (Illumina). RNA sequencing was done in the Core Facility for Scientific Research  University of So Paulo (CEFAP-USP/GENIAL).