title: A. cephalotes NPA/VKR KD in MAJ, GFPi treatment,  24h, r5
design: total RNA was isolated via Trizol extraction from dissected tissues or cultures, followed by DNase treatment and re-purification using RNA clean XP beads with a ratio of 2:1.  Poly-A selection was performed using NEB next kit, followed by directional library preprartion.  libraries were PCR cycled for 6-10 cycles depending on prior qPCR quantification of adapter-ligated cDNA.  all comparisons were cycled from the same number of PCR cycles to ensure comparability.