title: WES of Homo sapiens, saliva
design: A low input library prep protocol developed at CIDR (Marosy et al) was performed. Libraries are prepared from 150 ng of genomic DNA, sheared for 105s using the Covaris E220 instrument (Covaris). The Kapa Hyper prep kit is used to process the sheared DNA into amplified dual-indexed adapter ligated fragments. All processing was done in 96 well plate formats using robotics (Beckman FXp, Perkin Elmer Janus, Agilent Bravo, Beckman NX). With Bead clean ups were used following shearing and adapter ligation. Amplified libraries were pooled (6-plex, 500 ng per library) prior to enrichment following the Twist protocol (24 hour hybridization). Post-enrichment PCR was performed using the Kapa HiFi enzyme according to the Twist protocol, with the adjustment of PCR cycles.