title: Genome sequence of Lacticaseibacillus paracasei
design: Genomic DNA was extracted using the MagAttract HMW DNA Kit (Qiagen, Germany) following the manufacturer's instructions. Nanodrop (ThermoFisher Scientific, USA) was used to measure the quality and concentration of DNA isolated. Illumina and Oxford Nanopore Technologies (ONT) libraries were run using the same DNA preparation. For Illumina sequencing, NexteraXT DNA Library Prep Kit (Illumina, USA) paired-end library (2x300bp) was constructed and sequenced on the Illumina MiSeq platform. For ONT sequencing, genomic DNA was fragmented with g-TUBE (Covaris, USA). Then, the library was built using the Native Barcoding Expansion Kit (EXP-NBD114) (ONT, UK) and sequenced on the ONT MinION platform with FLO-MIN106D flow cell vR9.4.1.