title: LMIC human fecal stool sample CAM005187-030670, whole-genome shotgun sequencing
design: DNA sequencing libraries were prepared using Illumina DNA Prep Tagmentation kit (Illumina, San Diego, CA), following the manufacturers instructions except for the following changes. The insert size was increased to a range of ~375-1100 bp by reducing the 1st and 2nd volumes of Sample Purification Beads to 40 l and 11 l, respectively. This modification resulted in larger inserts compared to the mostly below 300 bp inserts obtained using the manufacturers protocol. Libraries were quantified in 10 l volume reactions and 90-s annealing/extension PCR, and then pooled and normalized to 4nM. Pooled libraries were re-quantified by ddPCR on a QX200 system (Bio-Rad, Hercules, CA), using the Illumina TruSeq ddPCR Library Quantification Kit and following the manufacturers protocols. Libraries were sequenced using a MiSeq Reagent Kit v2 (500-cycles) on a MiSeq instrument (Illumina) at 16 pM, following the manufacturers protocols.