title: 16S rRNA amplicon sequence of anaerobic digester: anaerobic sludge and pretreated swine manure at SI ratio 1 gVS/gVS and 15%TS (final)
design: 0.25 g of fresh sample (total wet weight) was used to extract DNA from microorganisms using the PowerSoil DNA Isolation Kit (MoBio Laboratories Inc., USA), following the manufacturers instructions. The V4 region of 16S rRNA gene was amplified sequenced in a Illumina MiSeq platform, using primers 515F and 806R.