title: WGS of blood: iPSCORE_64_1
design: Genomic DNA was isolated from blood (or in 19 cases directly from the fibroblasts, DNEasy Blood & Tissue Kit), quantified, normalized, and sheared with a Covaris LE220 instrument. The samples were normalized to 1ug and submitted for whole genome sequencing. DNA libraries were prepared (TruSeq Nano DNA HT kit, Illumina), characterized in regards to size (LabChip DX Touch, Perkin Elmer) and concentration (Quant-iT, Life Technologies), normalized to 2-3.5nM, combined into 6-sample pools, clustered and sequenced on the HiSeqX (150 base paired-end). In total, germline whole genome sequencing (WGS) was performed for 274 subjects though only 222 were reprogrammed into iPSCs. WGS was performed at Human Longevity (HLI).