title: The whole genomic sequence of Staphylococcus pseudoxylosus Hao 2022: sea water
design: Illumina NovaSeq 6000 is a high-throughput gene sequencing platform based on advanced next-generation sequencing technology. Here is a brief description of how it works:1. Sample preparation: First, DNA or RNA samples are extracted from biological samples and undergo specific pre-treatment steps, such as library construction, so that the sample can be efficiently identified and amplified during sequencing.2. Library construction: DNA or RNA samples are processed into libraries, which involves connecting DNA fragments or RNA transcripts to specific sequencing adapters for subsequent amplification and sequencing.3. Cluster Generation: Before the sequencing process begins, DNA fragments or RNA transcripts in the library are fixed to solid phase vectors and amplified by PCR or bridge PCR to form millions of clusters. These amplification clusters are the basic units for subsequent sequencing, and each cluster contains thousands of copies of the same DNA fragment or RNA transcript.4. Sequencing: The amplification clusters are located on the surface of the sequencing chip and sequenced one by one through fluorescent signals. The NovaSeq 6000 uses Illumina's unique sequencing chemicals and processes to enable high-throughput and high-accuracy sequencing. Each base addition is recorded by a camera, generating raw sequencing data.5. Data analysis: The original sequencing data goes through computer processing and bioinformatics analysis, including sequence alignment, variation detection, genome reconstruction and other steps, and finally generates data results that can be analyzed by researchers or clinicians.