title: MiSeq of coastal seawater metagenome: LMPB
design: Seawater samples were collected in a coastal ocean observatories (Equatorial Atlantic Microbial Observatory) from 2013 to 2016. DNA extraction was carried out with phenol-chloroform protocol and subsequent purified using Amicon column. PCR amplification was performed using primers for the 16S rRNA gene V4-V5 region. Samples were sequenced in an Illumina MiSeq platform.