title: RNA-seq of Arabidopsis thaliana: cxip4-2_replica2
design: Generated at Novogene (United Kingdom). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the synthesis of the second strand cDNA using dTTPs. The cDNA library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.