title: Human K562 cFOOT ATAC
design: cFOOT-ATAC-seq involves enzymatic deamination followed by Tn5 transposase treatment to fragment open chromatin regions while preserving conversed DNA base within these regions. Subsequent library preparation utilized the Illumina V3 EpiArt DNA methylation kit, incorporating primers designed to match the adapter sequences integrated by Tn5 transposase. In brief, single-stranded DNA was ligated with adapter sequences at the 3' end, followed by initial amplification using universal 3' primers and primers specific to the Tn5 transposase adapter sequences. This step involves introducing universal adapters compatible with the Illumina next-generation sequencing platform at the 5' end of the Tn5 adapters via overlap PCR. Finally, universal primers were used for the ultimate library amplification.