title: Sample from liver tissue of Marine medaka after 14 days of feed brine shrimp
design: RNA-seq transcriptome librariy was prepared following Illumina StrandedmRNA Prep, Ligation from Illumina (San Diego, CA) using 1g of total RNA. Shortly, messenger RNA wasisolated according to polyA selection method by oligo(dT) beads and then fragmented by fragmentation bufferfirstly. Secondly double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit(Invitrogen, CA) with random hexamer primers (Illumina). Then the synthesized cDNA was subjected to end-repair, phosphorylation and A base addition according to Illuminas library construction protocol. Libraries were sizeselected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified usingPhusion DNA polymerase (NEB) for 15 PCR cycles. After quantified by Qubit 4.0, paired-end RNA-seqsequencing library was sequenced with the NovaSeq 6000 sequencer (2  150bp read length).