title: 16S rRNA gene sequencing of macroalgae microbiome collected in Antarctica
design: The DNeasy PowerSoil Pro Kit (Qiagen, Germany) was used to extract the microbial metagenomic DNA, following the manufacturer's protocol. 16S V4 amplicon sequencing of the microbial community was performed on the Illumina MiSeq (2 x 300 bp) using the 515F-Y "new EMP" (GTGYCAGCMGCCGCGGTAA) and 806RB "new EMP" (GGACTACNVGGGTWTCTAAT) primer pair.