title: ddRAD seq of Bolitoglossa flaviventris SMR3244: adult liver
design: We extract genomic DNA from preserved liver  in NAP buffer we collect in field or flash-frozen tissues from museums following the method from (Aljanabi & Martinez, 1997). We modified the protocol from Jones et al. (2018) for ddRAD library prep using EcoRI and SpHI for double digest and a fragment size between 300-500 bp prior to sequence in a Novaseq 6000 platform 2x150 PE