title: RNA-Seq of Procambarus clarkii eyes
design: The cDNA libraries were conducted by using a TruSeq RNA Sample Prep Kit (Illumina, USA). mRNA was enriched by Oligo (dT) beads and fragmented into short fragments using fragmentation buffer and reverse transcripted into cDNA with random primers. The ligation products were size selected by agarose gel electrophoresis, PCR amplified, and sequenced using Illumina HiSeq2500 .