title: Effective capturing chloroplast RNA through poly(mA) tailing for nanopore direct total RNA sequencing
design: Total or oligo-dT enriched mRNA samples were used for poly(mA) tailing, poly(A) tailing or poly(I) tailing. The custom-tailed RNA was then used for nanopore direct RNA library construction. Sequenced RNA reads from each library were base called through Guppy base caller and mapped to Populus chloroplast genome through minimap2.