title: RNA-seq of Homo sapiens: pancreatic tumor sample: Sample 7T
design: RNA-SEQ using RNA isolated from FFPE tissue slices. RNA was extracted using the Qiagen RNAeasy kit, after DNase I treatment and rRNA depletion (Takara Bio RiboGone Mammalian kit), cDNA was made using the Takara Bio SMARTer Universal Low Input RNA kit and the library was prepared using the Ion Plus Fragment Library kit from Thermo Fisher Scientific. All cleanup steps were performed using Agencourt Ampure XP beads. Emulsion and enrichment steps were done on the Ion Chef, and the samples were sequenced on an Ion Torrent S5xl, using 540 chips, in the ATG facility at the University of New Mexico Comprehensive Cancer Center. Libraries were multiplexed (generally 4 samples).