title: PCR amplification and Oxford Nanopore Technologies sequencing of vmp expression site in Borrelia miyamotoi following in vitro antibody killing and reisolation.
design: The vmp expression site of B. miyamotoi LB2001 exposed to different antibody killing conditions and controls were PCR'ed with primers Pexp-F and Vlp25R and sequenced using ONT's Mk1B MinION with an R10.4 flow cell and the SQK-NCB112.24 library prep kit. The demultiplexing scheme is as follows: B01 Replicate 1 reisolate R2, B02 Replicate 1 no serum control (NSC), B03 Replicate 2 reisolate (R3), B04 round2 reisolate 1:5 high, B05 replicate 2 normal mouse serum control (NMSC), B06 Replicate 2 no serum control (NSC). A raw, concatenated FASTQ file of basecalled reads is given here for the entire sequencing run.