title: DNA from leaf samples
design: Leaf samples of Quillaja brasiliensis were obtained from germinated seeds collected from mature trees in Cangucu, RS, Brazil. Seedlings were kept under white fluorescent lamps at 25 +- 2 C on a 16/8 h day/night cycle and leaves were collected 11 months after sowing. The voucher specimen was deposited at the Herbarium of Federal University of Rio Grande do Sul (ICN 142953). We performed DNA extraction following the protocol of Healey et al. (2014; PMID: 25053969) adapted for recalcitrant plants. Genomic DNA was sequenced using a NovaSeq instrument (Illumina).