title: soil nirS
design: The Illumina Hiseq (Allwegene, Beijing) platform was used to target ammonia oxi-dizing archaea (AOA) amoA, AOB amoA, nirS, and nirK genes Perform high-throughput sequencing. The raw sequencing data were filtered and pruned using PReprocessing and INformation of SEQuence data (PRINSEQ) and Moetur tools. The UCLUST tool in Quantitative Insights Into Microbial Ecology (QIIME) was used to conduct a cluster analysis on sequences with a sequence similarity threshold of 97% and calculate the Al-pha Diversity Index (including Shannon and Chaol) for each set of samples.