title: Mu-seq 8: DNA-seq of 24 pooled mutagenized F2-families of maize (B73 background) ; Zea mays; Row-11
design: Seedling leaves (10 dag) harvested and pooled (24 Col pools x 24 Row pools = 576 F2-mutagenized families of maize). Leaf gDNA extraction and fragmentation. 1st PCR: enrichment of Mu-flanking DNA. 2nd a 3rd PCR to incorporate adapter sequences and barcodes. Sequencing fragments are: CAAGCAGAAGACGGCATACGAGATnnnnnnGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT--DNAinsert--AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT. The "nnnnnn" refers to the index.