title: 16S rRNA amplicon sequencing of human gut microbiome
design: Microbial DNA was extracted using the HiPure Stool DNA Kits. The 16S rDNA V3-V4 region of the ribosomal RNA gene was amplified by PCR using primers 341F/806R (341F: CCTACGGGNGGCWGCAG;   806R: GGACTACHVGGGTATCTAAT).   Amplicons were extracted from 2% agarose gels and purified using the AxyPrep DNA Gel Extraction Kit according to the manufacturers instructions and quantified using ABI StepOnePlus Real-Time PCR System.   Purified amplicons were pooled in equimolar and paired-end sequenced (PE295) on an Illumina platform according to the standard protocols.