title: RNA-Seq of mus musculus: female hippocampus
design: The hypoxia-reoxygenation cycle was used to simulate CIH in OSAS. snRNA-seq was performed to identify cellular subsets in the hippocampus of CIH mice, and cellular communication analysis was performed to find out intercellular signaling mechanisms. Bulk RNA-seq was integrated to identify DEGs to further identify genes and signaling pathways associated with microglial polarization in CIH mice. Expression levels of hub genes were identified using quantitative real-time PCR (qRT-PCR) and immunofluorescence.