title: 16S-FRD-Pilot-C-3 20-30 cm
design: Field trial phase - sterile 15 cc Falcon tubes were filled with material from each sampling point / location and submerged in LifeGuard Soil Preservation Solution (Qiagen) prior to transport from Johannesburg to Australia. Using a laminar flow cabinet, 0.5 g subsamples were taken from each system using a sterile spatula and sent for DNA extraction, amplification of 16S rRNA genes and sequencing using the Illumina Miseq platform at the Australian Centre for Ecogenomics as mentioned above.