title: RNA-seq of Eleutherodactylus coqui: adult skin
design: Only samples with RIN>7.0 were used for mRNA isolation, directional RNAseq library preparation, and Illumina 150 bp paired-end sequencing. Briefly, after the QC procedures using bioanalyzer, mRNA was isolated using oligo(dT) beads and rRNA was removed using the Ribo-Zero kit. The isolated mRNA was used for cDNA synthesis and 150 bp pair-end sequenced directionally.