title: Single-cell RNA Seq of transcformed PARCB tumor cells
design: Single cell gene expression libraries were created using Chromium Next GEM Single Cell 3' (v3.1 Chemistry) (Cat# PN1000123, 10x Genomics), Chromium Next GEM Chip G Single Cell Kit (Cat# PN1000120, 10x Genomics), and Single Index Kit T Set A (Cat# PN1000213, 10x Genomics) according to the manufacturer's instructions. Briefly, cells were loaded to target 10,000 cells to form GEMs and barcode individual cells. GEMs were then cleaned cDNA and libraries were also created according to manufacturer's instructions. Library quality was assessed using 4200 TapeStation System (Cat# G2991BA, Agilent) and D1000 ScreenTape (Cat# 5067-5582,  Agilent) and Qubit 2.0 (Cat# Q32866, Invitrogen) for concentration and size distribution.