title: May_RF_Inner_Rep3
design: The WT strain May1244  was lawned on RPMI solid media and cultured statically for 48hrs at 37C in the presence of a fluconaozle Etest strip. Cells were harvested from the zone of inhibition surounding the ETest by washing with sterile PBS and RNA was then extracted using the RNeasy extraction kit (QIAGEN) as described previously by Fletcher et al., PMC10721199