title: RNA-Seq of PBMCs treated IronQ
design: mRNA was purified from total RNA through oligo dT selection and fragmentation, followed by reverse transcription into first-stand cDNA using random hexamer primers. The resulting first-stand cDNA serves as a template for synthesis of double-stranded cDNA. Following the end repair process, the double-stranded cDNA underwent PCR amplification and subsequent purification to generate a cDNA library.