title: Transcriptome sample Folsomia candida _ 9.8 mg/L acetamiprid
design: The RNA samples were resuspended in 30uL RNase free water. The concentration of total RNA was determined using the Qubit RNA Assay Kit (Life Technologies). Poly-A selection and library preparation  were performed by using the KAPA mRNA HyperPrep Kits (Roche) by following the manufacturer's instructions. 700-1000 ng RNA was used to prepare the library. Following the library preparation, the final concentration of the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size were determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were then diluted to 0.7nM, and sequenced paired end for 300 cycles using the NovaSeq 6000 system (Illumina).