title: scRNA-seq of B16 tumor - CD45+ sorted cells
design: From each animal, 20,000 CD45+ live cells were sorted using a five-laser MoFlo Astrios EQ jet-in-air sorting flow cytometer, and samples from the same treatment were pooled. Next, 10,000 cells per condition were targeted for droplet formation using a Chromium Single Cell System (10x Genomics). Samples were processed according to the manufacturers protocol (10x Genomics, Chromium Next GEM Single Cell 3 Library).