title: RNA-seq of mus musculus with covid: bone marrow megakaryocyte
design: Femurs from female K18-ACE2 mice infected with SARS-CoV-2 for 3 days were fixed in formalin 24h, decalcified 14 days with EDTA and embedded in parafin. 10m sections were prepared and mounted onto Leica Bond Plus microscope slides.  DSP was performed by NanoString Technologies using GeoMX platform with the NanoString Mouse Whole Transcriptome Atlas. Immunofluoresence staining was performed using anti-CD41 and anti-endomucin. ROI was selected based on the fluoresence in interest (CD41) and whether megakaryocytes were not adjacent to sinusoid vessels.