title: 16S RNA-Seq of mus musculus: stool
design: 16S rRNA gene amplicon sequencing was performed on stool isolated from mice using protocols adapted from the Earth Microbiome Project, as previously described (Lobel et al., 2020). From extracted fecal DNA, the 16S rRNA V4 region was amplified by PCR with a single-indexing approach and indexing barcodes as listed in Table S10. Sequencing was performed on a MiSeq instrument (Illumina, San Diego, CA) using 1x150bp paired-end protocol (Mid Output: 10M clusters) by the Molecular Biology Core Facilities (MBCF) at Dana-Farber Cancer Institute (DFCI).