title: RNA-Seq of kangaroo rat: anal swab 3
design: DNA extraction was performed directly using MagAttract PowerSoil DNA KF Kit (QIAGEN cat.no. 27000-4-KF) with the Kingfisher Flex DNA extraction machine following the Earth Microbiome projects protocol. The extracted DNA were then shipped to Research Technology Support Facility Genomics Core at Michigan State University for DNA library preparation and Illumina amplicon sequencing of the V4 region of 16S using the MiSeq V2 Standard platform producing 250 bp paired end reads.