title: honeybee-RNA-seq
design: Total RNAs were extracted from the ovary and testis samples using Trizol Reagent (US Patent No.5,346,994). The extracted total RNA purity (OD260/280), concentration, nucleic acid absorption peak, and RNA integrity were detected using NanoDrop 2000 (Thermo Fisher Scientifc, Wilmington, DE) and Agilent Bioana lyzer 2100 system (Agilent Technologies, CA, USA). The mRNAs were then enriched using magnetic beads with oligo (dT) while being randomly interrupted in fragmentation bufer to construct the sequencing libraries.