title: 16s RNA of microbiota of Micropterus salmoidesfed HE005 diet3
design: the microbial DNA was extracted by using the E.Z.N.A. Soil DNA kit (Omega Biotek, Norcross, GA, U.S.). The V3-V4 region of the bacterial 16S ribosomal RNA gene was amplified by PCR using the primers 338F 5-ACTCCTACGGGAGGCAG CAG-3; 806R 5-GGACTACHVGG-TWTTAAT-3. Then, amplicon DNA was extracted and purified, and SMRTbell libraries were prepared by blunt-end ligation(Pacific Biosciences).