title: HSFB_Input
design: DAP-seq was performed by Bluescape Hebei Biotech using genomic DNA (gDNA) purified from the leaves of 30-day-old B73 seedlings with a FastPure Gel DNA Extraction Mini Kit (Vazyme) and sonicated to 200-bp fragments. After end-repair and adenylation, the fragmented gDNA was constructed into sequencing libraries using a NEXTFLEX Rapid DNA Seq Kit (PerkinElmer, Inc., Austin, TX, USA). The full-length coding sequence of ZmHsf20 was cloned into the pFN19K HaloTag T7 SP6 Flexi vector, and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega) were used to purify and capture the produced protein. The ZmHsf20-bound beads were incubated with the adapter-ligated gDNA library. The beads were then washed 3 times,and the eluted DNA was ligated to an indexed adaptor for sequencing analysis. The eluted DNA from the beads was sequenced on an Illumina NavoSeq6000 instrument with two biological duplicates. The negative input control consisted of Magne Halo Tag Beads incubated with adapter-ligated gDNA library.