title: Whole genome sequencing of Haloarcula hispanica ATCC33960
design: Culture was innoculated from a single colony into rich media (23% salt YPC) and grown to mid-log phase at 37C. Genomic DNA was extracted using the spooling method as described in 2009 edition of the Halohandbook (https://haloarchaea.com/halohandbook/). Purified DNA was quantified using a NanoDrop system (Thermo Scientific) and sonicated in a Diagenode sonicating water bath for 20 cycles on high. DNA quality was assessed by using a bioanalyzer using a high-sensitivity DNA chip (Agilent). Samples were submitted to Duke Center for Genomic and Computational Biology core sequencing facility for adapter ligation with TruSeq (Illumina) adapters and library amplification.