title: A16C4.1
design: Readfq (V8, https://github.com/cjfields/readfq) is used for preprocessing RawDatafromtheIllumina sequencing platform to obtain Clean Data for subsequent analysis. The specific stepsareasfollows: a) Remove reads with low-quality bases (default quality threshold is < 38) that exceedacertain proportion (default length is 40 bp); b) Remove reads with N bases reachingacertainproportion (default length is 10 bp); c) Remove reads whose overlaps with adapters exceedacertainthreshold (default length is 15 bp).