title: UCE targetted enrichment of Mergus merganser: muscle
design: We extracted DNA using the QIAGEN DNeasy Blood + Tissue Extraction Kit following manufacturers protocol. In short, we prepared Illumina dual-indexed DNA libraries which were checked for size distribution on 1.5% agarose gels and quantified using a Qubit fluorimeter. Libraries were prepared using iNextEra protocols. We then enriched pools of 8-12 samples with 5,060 UCE loci using the Tetrapods-UCE 5Kv1 kit from MYcroarray following manufacturers protocol version 5.01. The resulting pools were then sequenced on Illumina instruments to obtain paired-end 150 base (PE150) reads.