title: soil organism
design: All experiments were divided into four treatments: (1) soil with no amendment (CK), (2) soil amended with 2% (w/w) biochar (BB), (3) soil amended with 2% (v/w) Pseudomonas putida seed liquid (Ps), (4) soil amended with 2% (w/w) biochar-loaded bacteria. All treatments were incubated in triplicates in a light incubator for 45 Days at 25 C for 14 h light, 10 h dark. Triplicates in each treatmentwere collected on Day 3, 5, 14, 28, 45 for metagenome analysis.