title: RNA-Seq of Amphiprion ocellaris: larva treated with CPF, T3, and IOP (8dph to 13dph)
design: RNA-Seq libraries were generated from 2000 ng of total RNA using the Illumina Stranded mRNA Prep mRNA Sample Preparation Kit with UDI indices (Illumina, USA) according to manufacturer's instructions. Surplus PCR primers were removed using AMPure XP Bead-Based Reagent (Beckman Coulter Life Sciences, USA). Final cDNA libraries were checked for quality and quantified using Qubit (ThermoFisher Scientific, USA) and Fragment Analyzer for size profiling (Agilent, USA), and concentration-normalised using KAPA Library Quantification Kit for Illumina Platforms (Roche, USA)