title: RNA-Seq of DEF cells
design: After total RNA was extracted by Trizol reagent kit (Invitrogen, Carlsbad, CA,USA), the RNA molecules in a size range of 1830nt were enriched by polyacrylamide gel electrophoresis(PAGE). Then the 3 adapters were added and the 36-44nt RNAs were enriched. The 5 adapters were then ligated to the RNAs as well. The ligation products were reverse transcribed by PCR amplification and the 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina HiSeqTM 2500