title: RNA-seq of Homo Sapiens: adult cancer cell line HCT116
design: Total RNA was extracted with the RNAeasy mini kit using 5-10M cells. 200ng of total RNA was input into the PCR-cDNA Barcoding Kit (Oxford Nanopore, SQK-PCB111.24). The barcoded libraries were pooled in equal molar rations and ~40fmol was loaded per PromethION flow cell (R9.4.1, Oxford Nanopore, FLO-PRO002). Sequence data was generated on the PromethION P-24 instrument. Data was collected for ~72-80 hours generating >100M reads per run.