title: Nextseq of grapes: cabfranc10
design: DNA was extracted from grape juice using the Omega E.N.Z.A HP DNA extraction kit and a two step PCR was used to prepare libraries. The 16S and ITS specific primer pairs were used to amplify the corresponding gene regions and the Nextera Indx kit was used for ligation. Samples were purified using ExoSapIT and equimolar solutions were pooled and the library was sequenced on an illumina Nextseq 1000.