title: RNA seq of  Pseudoeurycea robertsi SMR2469: adult female liver, heart, spleen, intestine and tail
design: We extract total RNA with trizol based method according to Chomczynski (1993) from preserved tissues in NAP buffer we collect in field. We modified the TruSeq RNA library Prep v2 (Illumina, San Diego, California) to minimize the RNA needed per library and to maximize the number of libreries by kit. After quality and quantity check with bioanalizer 2100 DNA1000 chip, we sequenced in a Illumina HiSeq 4000 100PE.