title: 16S rRNA gene sequencing of A. thaliana leaf: NG2 WT
design: A two-step PCR was used to amplify the V3-V4 regions of the 16S rRNA gene, followed by a magnetic bead cleanup. Indexed primers used a dual-indexing scheme. PCR products were pooled based on fluorescence measurements (PicoGreen). The final library was sequenced on a MiSeq with 10% PhiX genomic DNA for 2x150bp cycles.