title: Short read RNA sequencing of Willaertia magna T5(S)44
design: For RNA extraction, the samples were collected in TRIzol Reagent (Invitrogen), and Phenol/Chloroform/Isoamyl alcohol extraction was combined with the RNeasy Mini Kit (Qiagen). Poly-A mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) from 500ng of total RNA. Enriched poly-A mRNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). The fragment size of the libraries was assessed using the NGS HS analysis kit and a fragment analyser system (Agilent). The library concentrations were quantified with a KAPA Kit (Roche). Poly(A) mRNA short read Illumina sequencing was performed using the NovaSeq SP PE150 readmode.