title: Low-coverage genome sequencing of Omaloplia nigromarginata X-DA0023b
design: DNA extracted with Qiagen blood and tissue kit (Qiagen, Hilden, Germany) was subject to shearing with a Bioruptor PICO sonicator (Diagenode), until the fragments had a peak size of 300  400 bp. The NEBNext Ultra II DNA library prep kit (NEB) was used to generate Illumina libraries (end-prep, adapter ligation), combined with the NEBNext 96 unique dual index primers (NEB). Paired-end Illumina (2x 150 bp) sequencing was done by a professional company and here yielded an output of 8-14 Gbp per specimen, as we aimed for a genome coverage of 5-10x.