title: DNA Sequencing of Escherichia coli : Ailuropoda melanoleuca fecal samples
design: Fecal samples from Ailuropoda melanoleuca were collected using sterile cotton swabs and spread on MacConkey agar. After 12 hours of incubation at 37C, suspected E. coli colonies were transferred to Eosin Methylene Blue agar for further confirmation based on colony appearance and sequencing results. Confirmed E. coli samples underwent DNA extraction and fragmentation, followed by library preparation using the MGIEasy Universal DNA Library Preparation Reagent Kit. The process included end-repair, dA-tailing, ligation, PCR amplification, and quality control. Finally, 150 bp paired-end sequencing was performed on the MGISEQ-2000 sequencer