title: Gut metagenome
design: Participants were instructed on how to self-collect the fecal samples using a training module and asked to return samples promptly to the local team. Samples were then stored in liquid nitrogen at the collection site and moved to a 80 C in Copan Ruinas, Honduras. Samples were shipped on dry ice to the United Stated of America and stored in 80 C freezers.  Stool material was homogenized using TissueLyzer from Quigen and the lysate was prepared for extraction with the Chemagic Stool gDNA extraction kit (Perkin Elmer) and extracted on the Chemagic 360 Instrument (Perkin Elmer) following the manufacturers protocol. Sequencing libraries were prepared using the KAPA Hyper Library Preparation kit (KAPA Biosystems). Shotgun metagenomic sequencing was carried out on Illumina NovaSeq 6000.