title: RNA-seq of Dermacentor variabilis adult salivary glands during feeding on rabbits
design: Intact salivary glands (SG) from partially fed Dermacentor variabilis adult females feeding on rabbits were collected at different stages during the blood feeding and stored in RNAlater solution. Ticks were grouped by weight, the average weight of the group 1 is 7.2 mg. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.