title: scRNA-Seq of spleen E19
design: Live human CD45+ cells were purified from porcine thymocyte cells by BD influx sorter. Cells were collected in PBS with 0.04% BSA and immediately counted using a LUNA-FL Dual Fluorescence Cell Counter (BIO-RAD, USA), and loaded in the 10x-Genomics Chromium (10x Genomics, CA, USA).