title: Genome sequencing of S. cerevisiae
design: Genomic DNA of all ancestral (A) and evolved (N) lines were isolated using Yeast DNA Extraction kit (Thermo Scientific) following manufacturers protocol. Briefly, overnight grown yeast cells were treated with lysing and protein removal agents (provided in the kit). The genomic DNA was then precipitated with isopropyl alcohol, the pellet washed with 70% ethanol and finally resuspended in 1X TE buffer (10 mM Tris), yielding about 35-70 ng/l DNA for each line. Sequencing was done using the Illumina Novaseq 6000 platform to obtain ~5 million paired-end 150bp (2  150bp) reads for each sample.