title: ITS1 gene amplicon of bovine: feces
design: Fecal samples were collected from rectum. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For fungal amplicon sequencing, primers targeting  fungal ITS1 gene (Edwards et al., 2008) were used.