title: RNA-Seq of Solanum lycopersicum: S2 ovaries
design: At least 4-5 Ovaries at stage S2 were then harvested into liquid nitrogen for RNA extraction (NucleoSpin RNA Plant, Macherey-Nagel, Germany). Each replicate was pooled from at least 5 plants. RNA-seq libraries were prepared using KAPA Stranded mRNA-Seq Kit (Kapa Biosystems, Wilmington, MA) and sequenced using Nextseq 2000 (Illumina, USA) for single-end 50bp reads.