title: RNA-seq of Helonias orientalis, method TR for onsite experiment
design: 300 bp fragment size strand-specific libraries were produced by NEBNext Ultra ll Directional RNA Library Prep Kit after mRNA was isolated by NEBNext Poly(A) mRNA Magnetic Isolation Module. Each sequence sample was sequenced 150 nucleotides at each end (2 x 150 bp paired-end) using Novaseq 6000 Sequencing System (Illumina Inc. San Diego, USA), resulting in more than 25 million raw reads.