title: 16s mus musculus
design: The genomic DNA was extracted using the QIAamp DNA Stool Mini Kit (Qiagen, Valencia, CA) modified to include bead-beating and RNase A treatment. Two steps of amplification were performed to prepare sequencing library using the AccuPrime Taq high fidelity DNA polymerase system (Invitrogen, Carlsbad, CA). 16S ribosomal DNA hypervariable region V4 was amplified using 20 ng genomic DNA and the gene-specific primers with Illumina adaptors.