title: genomic sequence of Arabidopsis thaliana Col-0 ino-4 mutant
design: Leaf samples were pooled and DNA extracted with Nucleon Phytopure DNA extraction kit (GE Healthcare, Pittsburgh, PA), according to the providers instructions. NEBNext DNA library components (New England Biolabs, Ipswich, MA) were used for fragmentation to 200-500 bp, and library preparation with Nextflex-96 indexes (Bioo Scientific, Austin, TX). Libraries were pooled and sequenced using the PE100 bp protocol on a single lane of Hiseq 2000. Reads were processed to remove tag and poor-quality regions using Sickle (Version 1.33, https://github.com/najoshi/sickle), and Scythe (https://github.com/najoshi/scythe), respectively.