title: HC1
design: RRBS library preparation was performed using the Acegen Rapid RRBS Library Prep Kit (Acegen, Cat. No. AG0422) following the manufacturer's protocol. The DNA samples initially underwent digestion using the methylation-insensitive restriction enzyme; then the fragmented DNA underwent a series of processes including end-repaired, A-tailed, and ligation to sequencing junctions where all cytosines were methylated; following this, fragments of DNA ranging from 150 to 300 base pairs were chosen for cleavage.