title: Single-cell amplified genomes of archigregarines
design: Single cells of archigregarines were manually picked from the intestines of marine invertebrate animal hosts, washed 2-4 times in ultrapure water, and isolated into 8 l of ultrapure water. After 2-3 freeze-thawing cycles to lyse the cells, they were then used as input into a TruePrime Single Cell WGA Kit (4basebio) with 12h of amplification time. Amplified gDNA was then purified using AMPure XP magnetic beads in a 1:1 ratio, and used as input into Illumina DNA prep for library preparation.