title: Targeted sequencing of Ripk1 mouse gene treated with AAV-SaCas9
design: DNA was extracted from liver sections using Macherey-Nagel NucleoSpin Tissue Extraction Kit with a final elution in 100l nuclease-free H2O. A 647 bp genomic region of the targeted locus was enriched by PCR before sequencing. Qubit quantitation and TapeStation assay were employed to quantify and determine the purity of the amplicon. Sequencing libraries were constructed following the NEBNext Ultra II (NEB) library preparation protocol.