title: Sequencing of HIV reverse transcriptase, protease and integrase: donor 133, LN Mig DC
design: The Sentosa SQ HIV Genotyping Assay involves seven main steps. Firstly, RNA extraction from virion-containing samples was performed with the Sentosa SX101 instrument (Vela Diagnostics, Hamburg, Germany), followed by an off-board RT-PCR step with a Veriti Dx 96-Well Thermal Cycler (Thermo Fisher Scientific, Waltham, MA, USA). Then, the Sentosa SX101 instrument built a pooled library, which underwent a template preparation step on the Sentosa ST401 system (Vela Diagnostics, Hamburg, Germany). After an enrichment procedure, performed with the Sentosa ST401e instrument (Vela Diagnostics, Hamburg, Germany), the sequencing step took place on the Sentosa SQ301 (Vela Diagnostics, Hamburg, Germany), which exploits the Ion Torrent system (Thermo Fisher Scientific, Waltham, MA, USA) to perform ultra-deep sequencing.