title: 16S-seq of feces
design: Briefly, DNA from the meconium and fecal samples were extracted using the DNeasy PowerSoil HTP Kit according to the manufacturers instructions. The V4 region of the 16S rRNA gene of the DNA extracted was amplified with a set of primers and molecular markers. PCR products with expected fragment size were cleaned using the UltraClean-htp 96-well PCR Cleanup kit (QIAGEN), and the 16S amplicons were sequenced on an Illumina HiSeq platform at the Dept. of Biochemistry and Microbiology located at Rutgers University (NJ/USA).