title: RNA-Seq of Troglodytes aedon: pooled blood
design: Total RNA was extracted in 2019 from avian blood samples (northern house wren, Troglodytes aedon) collected in 2017 and stored in a -80 degree celsius freezer until extraction. Blood was pooled across 10 adult male, adult female, and hatch-year (sex unknown) birds and a sequencing library was created using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.