title: Helix nucula isolate 7057 WGS
design: Sequencing libraries were prepared from total DNA with the NEBNext Ultra II FS DNA Library Prep Kit for Illumina and sequenced on the NextSeq2000 platform (150 bp paired-end). The reads were trimmed, filtered and deduplicated with TrimGalore 0.6.6 with cutadapt 4.6 (--length 140 -q 20 --stringency 1 -e 0.1) and fastp 0.23.1(--detect_adapter_for_pe --qualified_quality_phred 15 --unqualified_percent_limit 40 --n_base_limit 5 --length_required 15 --dedup --trim_poly_g).