title: TCR sequencing of Homo Sapiens : FVSNGTHWF (SARS-CoV-2) specific CD8 T cells.
design: PBMCs were stained with antibodies targeting CD4, CD8 and incubated with peptide-HLA tetramers consisting of FVSNGTHWF presented by HLA-B*35:01. Cells were first magnetic-activated cell sorted (MACS;miltenyi) for tetramer binding and then sorted using fluorescence-activated cell sorting (FACS;Aria II cell sorter, BD Biosciences) on CD4-CD8+tetramer+.