title: mRNA-Seq of human mitochondrial myopathy patients: muscle baseline
design: Total RNA was extracted from muscle samples using the Qiagen miRNeasy kit according to manufacturers instructions. RNA integrity and quantity was assessed on an Agilent Tapestation system (minimum requirements: RIN >7, >2000ng RNA and [RNA] >50 ng/l). mRNA libraries were created using the Illumina TruSeq Stranded mRNA kit and sequenced on the Illumina NextSeq 500 platform following the manufacturers protocols