title: Amplicon of ensiled filter cake 100 days rep3
design: For DNA extraction, samples were homogenized by vortexing, and an aliquot of 800 mg of solid samples and 600 l of liquid samples were used for triplicate extractions. The DNeasy PowerSoil Pro kit was employed following the manufacturer's instructions. Quantification of the samples was performed using a Qubit 3.0 Fluorometer (Life Technologies), and quality assessment, based on the 260/280 ratio, was determined using the NanoDrop Lite (Thermo Scientific)