title: DNA-Seq of soil metagenones: urban vs natural arid
design: Total soil genomic DNA was extracted using a DNeasy PowerLyzer PowerSoil Kit (Qiagen) following manufacturers instructions. Genomic DNA was fragmented to 500 bp length and ligated to Illumina adapters using the QIAseq FX DNA Library Kit (Qiagen, Hilden, Germany). Quality and quantity were determined with Agilent 4150 TapeStation DNA bioanalyzer. Samples were shotgun-sequenced on a 2150 bp Illumina NextSeq550 platform at the Microbiome Core of the University of Arizona. Quality of raw reads was evaluated using FastQC v.0.11.9 (Andrews 2010) and used to estimate of the parameters for adapter removal adapters with bbduck (Bushnell 2022)and quality filtering with Trimmomatic v. 0.38 (Bolger et al. 2014) (reads shorter than 50 bp and low-quality bases were removed).