title: 16S of Homo sapiens: feces
design: DNA extracted from 100-200 mg faeces content using Qiagen Powersoil DNeasy kit. DNA concentrations measured using Qubit 2.0 dsDNA HS kit (Qiagen) and adjusted to 5 ng/L before PCR. V3-region 16S rRNA genes was sequenced using the Ion Torrent GSS5 platform in DTUs DMAC core as previously described (Christensen et al. 2014).