title: 16S RNA of microbial community: soil sample
design: Purified DNA preparations were used to create libraries of 16S rRNA gene fragments by PCR using universal primers for the V4 variable region: F515/R806 (GTGCCAGCMGCCGCGGTAA /GGACTACVSGGGTATCTAAT), with attached adapters and unique Illumina barcodes. PCR products were purified according to the method recommended by Illumina using AMPureXP magnetic particles (BeckmanCoulter, USA). Library preparation and sequencing were carried out in accordance with the manufacturer's recommendations for operation on the Illumina MiSeq instrument (Illumina, USA) using the MiSeq ReagentKit v3 (600 cycle) with double-sided reading (2*300 n). Initial data processing, namely, sample demultiplexing and removal of adapters, was carried out using Illumina software (Illumina, USA).