title: Fecal samples in rats
design: After a week of adaptation, 30 animals were randomly divided into five groups (n = 6) as the following groups: (1) Cons: fed a regular diet; (2) Pos: fed an HFD; (3) Low: fed an HFD (Goji 1.5 g/kg.d per rat); (4) Mid: fed an HFD (Goji 3 g/kg.d per rat); and (4) High: fed an HFD (Goji 6 g/kg.d per rat).All rats were sacrificed after 10 weeks, and feces were obtained and preserved at 80 for 16S rDNA gut microbiota analysis.