title: Whole Genome Sequencing of Escherichia coli: Isolate ec125
design: In brief, volumes of 1ml, 10ml or 100ml were filtered through 47 mm 0.45 m nitrocellulose membrane and placed on selective media. Tryptone -glucuronidase Agar, TBX agar (Oxoid, UK) plates were incubated at 30C for 4 h followed by incubation at 44C for 14 h. Isolates were selected according to morphology and manufacturers instructions. Pure colonies were inoculated onto 3 ml nutrient agar slopes. Genomic DNA was extracted following the QIAamp DNA Mini kit protocol. Libraries were prepared with the Nextera XT DNA sample preparation kit from Illumina and sequenced using one lane on a NovaSeq 6000, with 250 cycles of paired-end sequencing.