title: RNA-seq, CTD26, replicate 4
design: Yeast cells grown in SC medium were harvested and homogenized with bead beating. Total RNA was isolated using the SPLIT RNA Extraction Kit (Lexogen) and treated with DNase I to eliminate genomic DNA. rRNA was depleted using the Ribocop for Yeast kit (Lexogen), and sequencing libraries were prepared with the CORALL Total RNA-Seq V2 kit (Lexogen). Libraries were sequenced on the Aviti platform (paired-end 275 bp). Adapter sequences were trimmed using Cutadapt.