title: LncRNA-seq of Arabidopsis thaliana: Col-0_replica1
design: Generated at Novogene (China). Messenger RNAs (mRNAs) and long non-coding RNAs (lncRNAs) were purified from total RNA by ribosomal RNA (rRNA) depletion using the Ribo-Zero kit. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the synthesis of the second strand cDNA using a custom buffer (Illumina), dNTPs, RNase H and DNA polymerase I. The cDNA library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.