title: RAD-seq of Sarotherodon melanotheron: gill
design: Library prepared using Illumina Nextera XT barcodes with 1 g DNA digested with the enzyme SbfI. Sequencing adapter ligation. P1 adapters used +  T4 DNA ligation. Ligated DNA samplespooled at equimolar concentration and sheared (1000 bp,Covaris). Size selection of the pooled DNA with Pippin HT. NEBNext ultra II DNA library preparation kit used for end repair, A-tailing and ligation of P2 adapters. Purification using AmpureXP beads. Amplification using Phusion High-Fidelity PCR Master Mix (12 cycles), purified with MinElute column to obtain 20L of sequencing library.