title: microbiome of moved dumped body
design: The V4 region of the 16S rRNA gene was isolated from microbial DNA with the barcoded primer set 515f/806r (Caporaso, J. G. et al. Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample. Proc Natl Acad Sci U S A 108 Suppl 1, 45164522 (2011).), following the protocol given by the Earth Microbiome Project (EMP) (Caporaso, J. G. et al. EMP 16S Illumina Amplicon Protocol. protocols.io https://www.protocols.io/view/emp-16s-illumina-amplicon-protocol-nuudeww (2018). AND  http://www.earthmicrobiome.org/emp-standard-protocols/) for library preparation.