title: The whole genomic sequence of Bacillus velezensis Hao 2023: sea water
design: ONT sequencing is a new generation of single molecule real-time electrical signal sequencing technology based on nanopore. The sequencing principle of each platform is the same. Led by the motor protein, the DNA/RNA strand binds to the nanopore protein embedded on the biofilm and decomhelices. Under the action of voltage difference on both sides of the biofilm, the DNA/RNA strand passes through the nanopore channel protein at a certain rate. Due to the differences in chemical properties of different bases on the DNA/RNA strand, So when a single base or DNA/RNA molecule passes through the nanopore channel, it causes changes in different electrical signals. By detecting and corresponding to these signals, the types of corresponding bases can be calculated and the sequence can be measured in real time.