title: V3-V4 region of 16S rRNA metagenome
design: Whole DNA present in all soil composite samples was extracted through a Soil DNA isolation kit. V3-V4 region of 16S rRNA gene was amplified through PCR. The amplified stretch of DNA products was purified and illumine, Miseq sequencing of V3-V4 region of 16S rRNA was performed with 2x300PE v3 sequencing kit. Then trimed sequences was analyzed for OTU abundance through QIIME/MOTHUR/KRAKEN/BRACKEN workflow.