title: Staphylococcus aureus genomic DNA
design: Libraries were prepared according to Illumina protocols. Libraries were quantified using the Promega QuantiFluor dsDNA System and run on a GloMax Discover Microplate Reader. Libraries were pooled following quantification in equal quantities. The final pool was double-SPRI size selected between 0.5 and 0.7X bead volumes using sample purification beads. The final pool was quantified on a Qubit 3.0 instrument and run on a D5000 ScreenTape using the Agilent Tapestation 4200 to calculate the final library pool molarity. The pool was run at a final concentration of 750 pM on an Illumina Nextseq20000 instrument.