title: Rhizosphere soil depth, soil physicochemical properties and microbial dynamics in two Peruvian superfood trees, custard apple, using PacBio-HiFi sequencing
design: The complete sequence of 16S RNA was amplified by the PacBio platform, which has the advantage of providing more accurate species-level information. The SMRTbell libraries (Pacific Biosciences) are prepared following the manufacturers user guide and sequencing performed at the Molecular Research LP (MR DNA) (Shallowater, TX, USA) on the PacBio Sequel following the manufacturers guidelines. After completion of initial DNA sequencing, each library undergoes a secondary analysis, using PacBios CCS (circular consensus sequencing) algorithm. The CCS algorithm aligns the subreads individually from each template to generate consensus sequences thereby correcting the stochastic errors generated in the initial analysis.