title: RNAseq of Mamestra configurata: carcass (after midgut removal) of 10 molting 4th to 5th instar larvae
design: Midguts were removed from 10 molting 4th instar larvae (soon to be 5th instar larvae), and the remaining carcases were immersed in TRIzol (Life Technologies). Total RNA was extracted by homogenizing in TRIzol reagent according to the manufacturer's protocol (Invitrogen). Total RNA was resuspended in sterile DEPC-treated H2O and quantified by measuring absorbance at 260 and 280 nm using a Nanodrop spectrophotometer (Fisher-Thermo).  RNA was run through the Micro poly-A purist column (Fisher-Thermo) to select for polyadenylated transcripts. The quality of the RNA was assessed using an RNA 6000 Nano Kit and a Bioanalyzer 2100 instrument (Agilent).454 sequencing libraries were synthesized and sequencing performed at the National Research Council, Saskatoon, Canada