title: RNA-seq of Helianthus tuberosus: tuber
design: High-quality total RNA was extracted from fresh root, quality checked by agarose gel electrophoresis and Nanodrop 2000 spectrophotometer, quantified by Qubit 2.0 and Agilent 2100 bioanalyzer. The mRNA was enriched by Oligo-dT beads, fragmented and reverse transcripted into cDNA and purified by AMPure XP beads, end-repaired, A-tailed, and adapter-ligated, purified and amplified by Illumina TruSeq RNA Library Prep Kit. The prepared library was quantified by Qubit 2.0 and Q-PCR and quality checked by Agilent 2115 before loading onto flowcell.