title: V3-V4 16s rRNA gene sequencing: human gastrointestinal tract samples
design: Amplicon library contstruction of V3-V4 16s rRNA gene was made with Herculase II Fusion DNA Polymerase using 341F and 805R primers and later Nextera XT Index Kit V2. Libraries were sequenced with Miseq 300bp PE; 100K reads per library