title: 16S RNA : Cyanobacteria, Heterotrophic bacteria
design: Amplifcation of  the variable V3-V4 region of 16S rRNA fragment using fusion primers U341F-MiSeq and 805R-MiSeq was perfomed. The purified PCR products V3-V4 used as templates for a next step PCR to include the indexes and the Illumina adaptors. The combinatorial use of index primers generated unique samples that were pooled and sequenced on one Illumina MiSeq run