title: RNA-seq of KN5585-1
design: For RNA-seq, the second leaves of V2 stage maize seedlings (Zmhsf20-1 and WT) exposed to heat at 45 for 24 h were used to extract total RNA with TRIzol reagent. The sequencing library was constructed using 1g total RNA from each sample. Libraries were prepared using the TruSeq RNA Library Preparation kit (Illumina, USA) and sequenced on an Illumina Novaseq platform, and 150-bp paired-end reads were generated.