title: WGS of Stenotrophomonas maltophilia
design: Genomic DNA was randomly fragmented. And The fragmented genomic DNA with certain average size were subjected to end-repair, 3 adenylated, and ligation with adaptors. Fragments were then used for PCR reactions. Single-stranded cyclized products were produced via denaturation and circularization program, while uncyclized linear DNA molecules are digested.