title: trimmed reads from small RNA Culicoides sonorensis 72 h post blood meal whole body rep2
design: 72 hours post blood-meal, whole females were collected. RNA extracted using TRIzol Phasemaker Gel Tubes was sent to the University of Kansas Medical Center Genomics Core for library preparation and Illumina sequencing. For each tissue sample, RNA quality was assessed with Agilent 2100 Bioanalyzer using the RNA6000 Nano kit chip. For Illumina library construction, 1g of total RNA was used as input and RNA libraries and generated directly from total RNA by capturing small RNA with 3OH and 5P modifications added by Dicer/Drosha. siRNA/miRNA RNA libraries were sequenced on a HiSeq2500 in Rapid Read. Post collection, sequence data was converted to FASTQ files and de-multiplexed into individual sequences for further downstream analysis. TruSeq Small RNA (TGGAATTCTCGGGTGCCAAGG).