title: Sequel II sequencing; Raw reads: AUZE-A-5_DNA
design: HMW-DNA (120 ng/µl), extracted from multiple inviduals, was sheared twice (back and forth) with a gTUBE (Covaris, PN 520079) in a Eppendorf Centrifuge 5424 at 4,800 rpm (soft) for 3 x 1 min. Five µg of sheared DNA was used for library construction with the HiFi SMRTbell Express Template Prep Kit 2.0 (PN 100-938-900) and SMRTbell Enzyme Clean Up Kit 2.0 (PN 101-932-600).  When multiplexed, libraries were combined in equal amounts and size-selected with the BluePippin system (SageScience) with 10 kb cutoff in a 0.75% DF Marker S1 High-Pass 6-10kb vs3 gel cassette (BLF7510, Biozym). The library pool was sequenced in a single SMRT Cell (30 hours movie time) following the loading and pre-extension recommendations (PN 101-769-100 version 6 to 9) with the Sequel II system (Pacific Biosciences) using the Binding Kit 2.2 (PN 101-894-200).