title: RNA-Seq of LX2 cells: FOXS1 knockout vehicle
design: LX2 (human hepatic stellate cells) FOXS1 KO and scrambled control cells were plated in 10% FBS 1%AA DMEM and allowed to grow to 80% confluency. Then, cells were treated with 5ng/mL TGFb or vehicle (10mM Citric acid) for 24 hours. Total RNA was harvested using the RNeasy kit (Qiagen). We quantitated the total RNA on a NanoDrop spectrophotometer (Thermo Fisher Scientific, Wilmington, DE), and samples were shipped on dry ice to Novogene (Sacramento, CA) for library preparation, quality control, and mRNA sequencing.