title: Illumina 75 bp paired-end genomic DNA library prepared from mixed age, whole-fly, female-producing female (X'X), adults, replicate 2
design: Genomic DNA was extracted using DNAzol (ThermoFisher) from 33 pooled whole-body individual X'X female-producing female adults. It was then quantified with the Qubit (ThermoFisher), analyzed for purity with Nanodrop (ThermoFisher), and analyzed for High Molecular Weight integrity with 0.5% agarose gel electrophoresis. It was prepared for Illumina sequencing using Illumina Nextera reagents, and was sequenced on the Illumina NextSeq 500 platform to generate 75bp paired-end reads from approximately 150- 400bp fragments.