title: MicroSatellite DataBase
design: PCR multiplexing of all selected markers was performed. The amplicons were amplified in a second step in order to attach Illumina adapters and dual indexes (8bp unique sequences) to each side of the amplicons.The second PCR-barcoded amplicons were pooled in a single tube, purified with 1.8X Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) and analysed on a on a Tapestation 4200 (Agilent, Santa Clara, USA). The final pool was quantified using QIAseq Library Quant Assay kit (Qiagen, Hilden, Germany) with a Roche LightCycler 480 quantitative PCR (Roche, Penzberg, Germany) and sequenced on an iSeq 100 system (Illumina, San Diego, USA) with a 2x150 bp kit.