title: 4
design: Total RNA was quantified with a spectrophotometer method and RNA integrity was evaluated with Agilent 4200 TapeStation analysis. RNA stranded libraries were generated starting from 400ng total RNA and mRNA was selected with poly-T oligo attached magnetic beads using New England Biolabs NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat. n. E7760S ). Libraries were quantified and quality checked on an Agilent 4200 TapeStation system (High Sensitivity D1000), and then sequenced on an Illumina NovaSeq 6000 platform with paired-end reads 150bp long, with a depth of 25 million cluster/sample.