title: 16S rRNA amplicon sequencing of Galaxea fascicularis: whole coral soft tissue
design: DNA was extracted from about 10 mg of coral tissue per sample using the Qiagen DNeasy 96 Blood & Tissue kit. Extractions followed the user manual with centrifugations at working steps 10, 12, and 16 were performed at 1500 g at doubled centrifugation times. Extracted DNA and a negative control sample (buffer without DNA) were sent for amplification and sequencing on the Illumina MiSeq platform at Bart's and the London Genome Centre, Queen Mary, University of London (UK). The 16S rRNA gene (regions V5-V6) was amplified using the primers 784F and 1061R (Andersson et al. 2008): forward primer 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGGATTAGATACCCTGGTA-3, reverse primer 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCRRCACGAGCTGACGAC-3.