title: 16S rRNA gene sequencing of Homo sapien: Elderly nasal wash sample
design: The 16S rRNA gene amplification and sequencing, targeting the V3-V4 hypervariable regions of the16S rRNA gene, was performed in the MiSeq system of Illumina at the facilities of Parque Cientficode Madrid (Tres Cantos, Spain) with the universal primers S-D-Bact-0341-b-S-17(ACACTGACGACATGGTTCTACACCTACGGGNGGCWGCAG) and S-D-Bact-0785-a-A-21(TACGGTAGCAGAGACTTGGTCTGACTACHVGGGTATCTAATCC), as previously described(Aparicio et al., 2020; Klindworth et al., 2013). The pooled, purified and barcoded DNA ampliconswere sequenced using the Illumina MiSeq pair-end protocol (Illumina Inc., San Diego, CA, UnitedStates).