title: 16S-Seq of seawater microbiome: littoral zone of the Black Sea
design: The sequencing library is prepared by random fragmentation of the DNA sample, followed by 5' and 3' adapter ligation. Adapter-ligated fragments are then PCR amplified and gel purified. For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment is then amplified into distinct, clonal clusters through bridge amplification. When cluster generation is complete, the templates are ready for sequencing.