title: GSM6499549: NC-RNA-seq-rep1; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq, total RNA was isolated using TRIzol reagent and was extracted following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent Bioanalyzer 2100. Qualified total RNA was further purified by RNAClean XP Kit. RNA-seq Library Prep Kit for Illumina® (Vazyme) was used to establish the library.Each group was sequenced with an Illumina HiSeq 2000. RNA libraries were prepared for sequencing using standard Illumina protocols