title: unpurified RNase-free water eluted low concentration sample
design: Whole transcriptome sequencing (total RNA-seq) data was generated using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Globin kit (PN 20020612). Globin mRNA and rRNA was depleted using rRNA & Globin removal beads then purified using Agencourt RNAClean XP beads (Beckman Coulter PN A63987).  Paired-end libraries were prepared on a Beckman BioMek FXp liquid handler using TruSeq kit reagents.  cDNA was poly-A tailed and then TruSeq UD indexes (PN 20022370) were ligated and amplified for 15 PCR cycles and then purified using AMPure XP beads (Beckman Coulter PN A63882).  Sequencing was completed on the NovaSeq 6000 using the S4 reagent kit to generate 2x150bp paired-end reads.