title: 16S rRNA gene amplicon sequencing of a methane-rich lake hypolimnion: Sequencing Blank
design: Polymerase chain reaction (PCR) was performed on ultrapure water as a negative control for sequencing. The V4 region of the 16S rRNA gene (Earth Microbiome Project (EMB) Caporaso 515F and 806R primers) was amplified by PCR with the EMB Illumina 16S amplicon protocol at the UT Genomics Core (The Center for Environmental Biotechnology at the University of Tennessee in Knoxville). The amplified 16S rRNA PCR products were prepared with an Illumina DNA prep kit and then sequenced at the UT Genomics Core using an Illumina Miseq.