title: LWR7, medium sequencing
design: We suspended each soil sample in water and rotated them on a gyratory shaker at 100 rpms overnight.  We extracted five separate, 10 milligrams aliquots using the PowerMax Soil DNA Isolation Kit according to the manufacturers recommendations.  We constructed total DNA libraries using the KAPA HyperPlus library preparation kit. We sequenced samples on an Illumina MiSeq using paired-ends with a 250bp insert size.