title: RNA-seq of CSA high binding P. falciparum NF54 parasites
design: Infected RBCs were collected at 16 h.p.i. and lysed with TRIzol Reagent (Life Technologies). RNA was purified using NucleoSpin miRNA Mini kit for miRNA and RNA purification (Macherey-Nagel) without size fractionation. Strand specific RNA-seq libraries were generated using the KAPA LTP library preparation kit (KAPA Biosystems). Libraries were amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) and purified using 1X KAPA Pure Beads (KAPA Biosystems).