title: H3K27me3 CUT and RUN on IPC purified from GW20 cerebral cortex: Sample S224-B11
design: Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 20 and GW16/17 using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~20,000 nuclei were  subjected to CUT&RUN using  antibodies to H3K27me3 histone modification. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq 6000.