title: RNA-seq of P. deltoides: leaf from genotype S7C8 in a Monoclonal planting
design: Frozen tissue samples of 100mg were ground to a fine powder in liquid nitrogen using the sterilized mortar and pestle. Total RNA was extracted using the Qiagen RNeasy plant mini kit (Qiagen, Genmantown, MD, USA) following the manufacturers instructions. he quantity and quality of the extracted RNA  assessed using a Nanodrop One Spectrophotometer (Thermo Fisher Scientific, 2023) and  on a 1% (w/v) agarose gel. RNA samples were sent to Novogene (www.en.novogene.com) for eukaryotic mRNA library creation and paired-end sequencing (PE150) with a total of 300 bp sequences for each RNA molecule was run on the Illumina NovaSeq 6000 (Illumina, San Diego, CA, USA).