title: Metagenomic nanopore sequencing ofCtenocephalides orientis pool  wCori_F8
design: Nanopore sequencing library was produced using the protocols for SQK-LSK109 Ligation Sequencing Kit and EXP- NBD104 Native Barcoding Kit (Oxford Nanopore Technologies) to allow for multiplexing. The sequencing libraries were loaded onto separate MinION Flow Cells (R9.4.1) on a MinION device. Post-sequencing, the basecalling of the fast5 reads and demultiplexing were performed using the Guppy basecalling software version 4.2.2 using high accuracy mode, with quality score cut-off at 9 and minimum read length filter set at 200