title: ChIP-seq of CSA high binding P. falciparum NF54 parasites using H3K9 antibody
design: Infected RBCs were collected at 16 h.p.i., lysed using 0.1% saponin (Sigma) and cross-linked in 1% methanol-free formaldehyde (Polysciences Inc). Chromaitin was sheared using the Covaris ME220 ultra sonicator and immunoprecipitated using anti-H3K9me3 antibodies (ab8898, Abcam). ChIP-seq libraries were prepared using the KAPA LTP library preparation kit (KAPA Biosystems), then amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) and purified with 1X KAPA Pure beads (KAPA Biosystems).