title: GSM8438916: M2c Wild-type Rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from macrophages by using RNeasy Micro kit (Qiagen, Hilden, Germany) based on manufacturer's instructions and as described (Li et al, 2023). Thereafter, RNA concentrations were determined (NanoDrop ND-1000, Thermo Fisher Scientific, Dreieich, Germany;  260 nm) 1.5 µg RNA was used as input for VAHTS Stranded mRNA-seq Library preparation following manufacture's protocol (Vazyme).