title: Targeted amplicon sequencing of maize root microbiome
design: Samples were amplified with primers for 16S (515-806R) and ITS1 (ITS1f and ITS2). For 16S rRNA gene PCRs peptide-nucleic-acid blockers were used to reduce non-target amplicons originating from chloroplast and mitochondria (Lundberg et al. 2013).