title: RNA-seq of Th2 cells from Bcl6 deficient vs WT CD4+ T cells during helminth infection
design: Total RNA from flow cytometry sorted CD4+GFP+CD44+CXCR5- cells at 2 weeks post Hpb infection was obtained with Qiagen RNeasy Mini kit following the manufacturers instructions. RNA was extracted using Trizol reagent (Sigma-Aldrich), and quality was checked with the Bioanalyzer RNA 6000 kit and sequenced using Novaseq 6000 sequencers (Illumina).