title: GSM6423518: OFO1005V2; Homo sapiens; RNA-Seq
library_construction_protocol: Drug treatment was conducted at 83-90 days of forebrain organoid differentiation and at 28-34 days of forebrain neuron differentiation. The concentrations of oxycodone (50 µg/L) and buprenorphine (2 ng/mL) used to perform these experiments were selected to fall within the range of blood drug concentrations in patients taking standard clinical doses of these two drugs. 3-D iPSC-derived brain organoids were cultured in the bioreactors with the drugs for seven days, and the medium was changed daily.