title: GSM6423006: MDM 18 hour; Homo sapiens; RNA-Seq
library_construction_protocol: in vitro derived macrophages from peripheral blood monocytes and induced pluripotent stem cells were extracted by washing cells with PBS before incubation at 37 degrees celsius for 5 minutes in 10mM EDTA. Single cell RNA-sequencing libraries were prepared using the Chromium Next GEM Single Cell 5' v2 (Dual Index) protocol as per manufacturer's instructions. Cell suspensions were prepared for a target recovery of 6000 cells per sample. Libraries were quality checked and quantified using the Agilent Tapestation D5000 High Sensitivity Tape.