title: fecal microbiota from fiber deficient diet-fed mouse #966 week 7
design: Amplicons of the V4 region within the 16S rRNA gene were generated and both ends of the fragments were sequenced. The paired end sequences were assembled into  250 bp contigs, aligned to the SILVA 16S rRNA database release 138, made free of possible chimeric sequences by UCHIME, binned into Operational taxonomic units at >97 % identity level and taxonomically assigned with 16S rRNA gene training set version 16 of Ribosomal Database Project.