title: RNA-Seq of anmmoxsludge
design: The 16S rRNA gene were amplified using the forward primer 338 F (5-ACTCCTACGGGAGGCAGCAG-3) and reverse primer 806 R (5-GGACTACHVGGGTWTCTAAT-3) targeting the V4 region. The 16S rRNA gene sequencing was performed by a commercial company (Majorbio, China) on Illumina MiSeq platform