title: DNA-Seq of Rouxiella badensis: DAR84756
design: A Single colony was grown overnight in PD broth at 28C while shaking at 200 rpm. The culture was then spun down and the pellet was used for DNA extraction through Promega DNA extraction kit. After checking the quality of the DNA it was used for both short read Illumina and long read Oxford Nanopore. The long read sequencing was generated by Garvan Institute using an Oxford Nanopore GridION platform with FLO-MIN106 flow cell. The SQK-LSK109 kit was used without shearing and the reads were called and quality controlled during sequencing using MinKNOW version 20.06.17 and Guppy version 4.0.11. This generated total of 268,783 reads. AGRF used Illumina 1.9 MiSeq platform with conventional base calling and produced 2,885,611 high quality reads with 251 bp length.