title: RAD-seq of Ammotragus lervia
design: Genomic DNA (gDNA,  200 ng) was extracted from 0.1 g liver or muscle or 100 l whole blood, using the Qiagen DNeasy blood and tissue extraction kit (Qiagen, Valencia, California).  Two restriction enzymes were used in RAD-seq: EcoR1 (6 base cutter) and Msp1 (4 base cutter).  ).  Samples were sequenced to a target coverage of 20 using the NovaSeq S4 instrument with 300 cycles (2  150 bp sequencing).  All paired-end DNA sequencing reads were demultiplexed by Admera Health (South Plainfield, New Jersey).