title: Amplicon Deep sequencing of peg-IPA1 treated by different prime editors: rice
design: For deep amplicon sequencing, two rounds of PCR were performed. In the first round PCR, PCR products containing the target site were amplified using gene-specific primers. In the second round PCR, primer sets with forward and reverse barcodes were used and limited the products size within 200 bp for library construction and Illumina sequencing.