title: NGS of bisulfite-treated DNA amplified from imprinted and non-imprinted loci in mouse: 9.5 dpc wild-type embryo
design: Mouse genomic DNA was treated with sodium bisulfite to convert unmethylated cytosines to uracil. Differentially methylated regions associated with imprinted loci and methylated regions of non-imprinted genes were amplified from bisulfite-treated DNA via PCR. Amplicons size was confirmed and products were purified using gel electrophoresis and gel extraction. Extracted products were quantified using a Qubit and pooled in equimolar amounts prior to NextGen sequencing.