title: Genomic profiling of imatinib resistant and sensitive K562 cells to obtain insights into imatinib resistance
design: Genomic profiling of imatinib resistant and sensitive K562 cells to obtain insights into imatinib resistance
library_construction_protocol: Gradual dose escalation protocol was used to establish imatinib-resistant K562 cell lines. K562 cells resistant to 0.25 μM, 0.5 μM, and 0.75 μM imatinib were maintained in cRPMI medium and the respective imatinib concentration and parental K562 cells were maintained in cRPMI medium only. Imatinib resistance was checked on the IC50 value for imatinib obtained from MTT assay. DNA was extracted from imatinib-sensitive and -resistant K562 cells, using AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, MD, USA: catalogue no. 80224) according to the manufacturer's recommendations. WGS was performed on imatinib-sensitive and -resistant (to 0.25, 0.5, and 0.75 M imatinib) K562 cells. Library preparation was done by 30x WGS KAPA PCR-Free v2.1 kit, as per manufacturers instruction.