title: Illumina NovaSeq6000 Sequencing of Staphylococcus capitis SCNIH009 sample Scap_PJ_017 Transcriptomic Paired-end Library
design: Illumina NovaSeq6000 2x151 sequencing of Scap_PJ_017 transcriptomic paired-end dual index library. Total RNA isolation using ZymoBiomcs DNA/RNA Miniprep Kit (R2002) followed by DNAase treatment and cleanup using NEB Monarch RNA Cleaning kit (T2030L). Ribosomal RNA was removed with Qiagen FastSelect -5S/16S/23S (335927). First and second strand cDNA synthesis with Illumina stranded total RNA prep kit (20040529) and cleanup with Agencourt AMPureXP beads (A63881). RNA-index anchors were ligated onto cDNA fragments followed by cleanup with AMPureXP beads. Dual-index libraries for Illumina sequencing were created from selective PCR amplification of anchor-ligated cDNA fragments. Libraries were assayed for quality and concentration by Agilent 2100 Bioanalyzer and DNA 1000 kit.