title: WGS of Saccharomyces: hybrid and parental species
design: We extracted genomic DNA from overnight YPD cultures derived from each clone according to the manufacturer's instructions of the MasterPure Yeast DNA Purification Kit, and purified on Axygen AxyPrep Magnetic PCR Clean-up SPRI beads. 5 DNA libraries were prepared using RIPTIDE High Throughput rapid DNA library prep in 96-well plate format (iGenomX, South San Francisco, USA). The quality of the libraries was verified using an Agilent BioAnalyzer 2100 electrophoresis system. Pooled libraries were sequenced using paired-end 150bp reads on different lanes of an Illumina NovaSeq 6000 at the Genome Quebec Innovation Center (Montreal, Canada).