title: GSM8333110: 293T_WT_ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq was performed as previously described (Li et al. 2021). Briefly, 5×104 cells were collected and washed with cold PBS and centrifuged at 500 g for 5 minutes. Then, the cells were lysed by 50 μl cold lysis buffer (Novoprotein, 10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl, 0.1% NP-40, 0.1% Tween-20, 1% Digitonin) and incubated on ice for 5 minutes. The cells were furthered added with 950 μl cold wash buffer (Novoprotein, 10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl, 0.1% Tween-20) and centrifuged at 500 g for 5 minutes The pellet was resuspended with 40 μl tagmentation buffer (Novoprotein, N248, 0.3×pbs, 0.1% Tween-20, 0.01% Digitonin, 1×TD buffer, 1×pA-Tn5 transposome Mix) incubated at 37 ℃ for 30 min. The reaction was stopped by adding 10 μl stop buffer (Novoprotein, N248) at 55 ℃ for 5 min. The DNA was further purified with 100 μl Tagment DNA Extract Beads (Novoprotein, N245) and eluted with 37 μl Elution buffer. The purifyied DNA was amplified with 5×AmpliMix (Novoprotein, N248) using N501 and N701 index primer((Novoprotein, N239). The purified DNA libraries were size selected with NovoNGS® DNA Clean Beads (Novoprotein, N240)