title: piglet_D32_S46
design: DNA library preparation was performed according to the TruSeq Nano DNA Sample Preparation Kit protocol (Illumina, San Diego, CA, USA). The samples were sheared on a Covaris S220 or E220 focused ultrasonicator (Covaris, Woburn, MA, USA) to approximately 450 bp, following the manufacturers recommendation, and uniquely tagged with one of the TruSeq LT DNA barcodes (Illumina). The finished libraries were quantitated using a Quant-iT PicoGreen dsDNA Kit (Thermo Fisher Scientific) and the average library size was determined using a Bioanalyzer 2100 instrument, using a DNA 7500 chip (Agilent Technologies). Library concentrations were then normalized to 4 nM and validated by qPCR on a ViiA-7 real-time thermocycler (Thermo Fisher Scientific), using the KAPA Library Quantification Kit for Illumina platforms (Roche, Basel, Switzerland).