title: The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation
design: The ER stress sensor IRE1 interacts with STIM1 to promote store-operated calcium entry, T cell activation, and muscular differentiation
library_construction_protocol: CAMPARI2 PC for screening was performed in 60 mm plates by lighting with 365, 385 420 nm Led light (Cholis High-Power Led source (Thorlabs) for 3 minutes at 25mW/cm2. We then FACS sorted the low 10% photoconverted cells,  Tunicamycin 100ng/ml was used for 4 hours before PC isolated their genomic DNA using JetQuickâ„¢ Blood and Cell Culture DNA Midiprep Kit (A30703, Thermo). See original de brie library please Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.