title: WGS of Animal passaged Streptococcus pyogenes M1 5448 knockout of streptolysin O with conferred covS mutation
design: DNA was extracted from a single colony using a QIAsymphony DSP virus pathogen kit (Qiagen).  Genome sequencing was performed using Nextera XT (Illumina) on the NextSeq 500/550 platform using 150 paired-end reads (Doherty Applied Microbial Genomics, Melbourne)