title: RNA-Seq of groundwater Wilcox Oil Superfund Site
design: Groundwater samples were collected from groundwater wells following protocols for low-flow groundwater sampling (Puls and Barcelona, 1996). Samples were isolated using a MoBio PowerMag Soil DNA Isolation Kit. DNA was extracted following MoBios instructions on a KingFisher extraction instrument. Bacterial 16S rRNA genes were PCR-amplified with dual-barcoded primers targeting the V4 following protocols in Kozich and others. (2013). Amplicons were sequenced with an Illumina MiSeq using the 300-bp paired-end kit (v.3).