title: RNA-seq of mouse pancreatic pericytes from MyD88-deficient and control mice
design: RNA-seq of mouse pancreatic pericytes from MyD88-deficient and control mice
library_construction_protocol: Pancreatic tissues were collected from pericytic MyD88 deficient (Nkx3.2-Cre;R26-EYFP;MyD88 flox/flox) and control (Nkx3.2-Cre;R26-EYFP) 12-week-old mice  For cell isolation, dissected pancreatic tissues were digested with Collagenase P (0.4 mg/ml; Roche) and DNase (0.1 ng/ml; Sigma) diluted in HBSS for 30 minutes at 37 C to obtain single cell. DAPI (1 l/ml; Sigma-Aldrich) was added to label dead cells. Live fluorescently-labeled cells were collected using FACS Aria (BD). Using PureLink RNA micro kit  Libraries were prepared using the SMART-SeqÂ® stranded Kit (Takara Bio) following the low-input protocol