title: GSM7245158: AVCE9,5; Mus musculus; RNA-Seq
library_construction_protocol: Avc was microdissected from E9.5 mouse embryos; mitral valve at E16.5 and postbirth ( P0, Neonate) were microdissected. Cells were digested with TRIPLE for 5 or 10 min , blocked with 10% decomplemnted FCS and FACS sorted using tomato libraries were prepared using the single cell 3' reagent kit from 10C genomics. Sequncing was carried out using NextSeq  500 1x57 nt