title: Bulked segregant analysis (BSA) of slrx620 (suppressor of lrx345-620) mutant based on genome sequencing
design: All steps of the isolation and library preparation workflow were performed according to the manufacturers protocol. Total RNAs were extracted from whole seedlings using an DNeasy Plant Maxi kit (QIAGEN). After digestion with NEB dsDNA Fragmentase, DNA library preparation workflow were performed using a NEBNext DNA Library Prep Reagent Set for Illumina according to the manufacturers instructions. End repair of fragmented DNA and cleanup using AMPure XP beads (Beckman Coulter). dA-Tailing of end repaired DNA and cleanup. Adaptor ligation of dA-Tailed DNA and cleanup of adaptor ligated DNA. Size select adaptor ligated DNA using AMPure XP beads. PCR enrichment of adaptor ligated DNA and cleanup.