title: RNA-Seq of Notothenia rossii
design: Only extracts exhibiting minimum RIN values  8 were chosen for subsequent processing. For sequencing library construction, the extracted RNA samples underwent conversion to complementary DNA using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA). we quantified the prepared libraries using qPCR as per the Illumina qPCR Quantification Protocol Guide.