title: 16S rRNA Sequencing of Mus musculus feces - SPF ctrl1
design: DNA was extracted utilizing Promega Max Prep & Promega Maxwell RSC 48 instrument.  Library generation follows the protocol from Earth Microbiome Project (http://press.igsb.anl.gov/earthmicrobiome/protocols-and-standards/16s/).  515F-926R primers were used to amplify 16S rDNA V4 & V5 Regions.  Library pool was sequenced PE250 on the Illumina MiSeq, at 7pM & 15% PhiX.