title: PI sample B
design: Total RNA was extracted from sampled tissues using TRIZOL solution and purified as per manufacturer guidelines. The RNA quality was verified using an Agilent bioanalyzer 2100 system, and only high-quality RNA samples were used for biological triplicates. cDNA libraries were constructed and sequenced by Genomix4life SRL using a TruSeq stranded mRNA kit and Illumina NextSeq 550 platform. The goal was to achieve around 20 million paired-end reads per sample through a 2x75 cycles sequencing strategy.