title: 16S rRNA gene sequencing of peat microcosms: anoxic incubation t4, 21d, replicate 5
design: Genomic DNA was extracted from peat soil originating from destructively sampled macrocosms. DNA was extracted using the DNeasy PowerSoil Extraction kit (Qiagen). A fragment of the 16S rRNA gene was amplified by PCR using the primer set 515F (5-GTGYCAGCMGCCGCGGTAA-3)-806R (5-GGACTACNVGGGTWTCTAAT-3). PCR products were used for library preparation with the NEBNext Ultra II FS DNA library preparation kit (New England Biolabs). Sequencing was performed inhouse on an Illumina MiSeq platform using v3 chemistry in paired-end mode (2 x 300 bp).