title: RNA-seq of C918 cells
design: For RNA-seq, C918 cells were collected and total RNA was then isolated. Subsequently, total RNA was subjected to RNA-seq analysis using the DNBSEQ system. For library construction, RNA was sheared and it was then reverse transcribed into cDNA using random primers. Subsequently, sequencing was performed using the prepared library on the DNBSEQ-T7  platform. The expression levels (FPKM, fragments per kilobase per million fragments mapped) of the target genes was calculated by RSEM. The read counts for each gene were determined using the SubRead package, while the normalization and differential expression analysis were performed using the DeSeq2 package.