title: RNAseq of wild-type and p53 null mouse embryonic stem cells at day 3 of differentiation
design: RNAseq of wild-type and p53 null mouse embryonic stem cells at day 3 of differentiation
library_construction_protocol: Cells grown for 3 days in N2B27 media were recovered into growth media and then resuspended in RLT lysis buffer (Qiagen).  RNA extraction was performed using the Qiagen RNeasy kit according to the manufacturerâ€™s instructions.  RNA samples were quantified using a Qubit fluorometer (Thermo Fisher Scientific) and the quality assessed by TapeStation electrophoresis (Agilent). mRNA was isolated using oligo dT beads. mRNA was then fragmented, converted to cDNA and ligated to Illumina adapters. Following sample indexing, the quality of cDNA libraries was also assessed by TapeStation.