title: RNAseq of Salmonella enterica serovar Infantis strain SI 1143/14 under increased osmolarity (LB broth with NaCl 9%)
design: The total RNA of the sample was extracted using the PureLink RNA Mini Kit (Thermo Fischer Scientific) following the kits protocol. Quantification of the RNA extracts obtained were performed using Qubit RNA Broad-Range Kit (Invitrogen). Quality control was performed using High Sensitivity RNA ScreenTape (Agilent Technologies). Depletion of ribosomal RNAs and RNA libraries were prepared using Illumina Stranded Total RNA with Ribo Zero Plus kit (Illumina). Clusterization and sequencing were performed in Illumina NovaSeq 6000 using NovaSeq 6000 SP Reagent Kit (200 cycles).