title: GSM7964849: Inguinal White adipose tissue, Day14, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Stromal vascular fraction of mouse iWAT were prepared by enzymatic dissociation with collagenase Ⅷ (sigma c2139). Cells were loaded into Chromium microfluidic chips and barcoded within a 10× Chromium Controller (10X Genomics), Single cell transcriptome were reverse-transcribed into cDNA library containing 10X cell barcodes and unique molecular identifiers (UMI) information. All procedures were performed with reagents from Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (10X Genomics, Cat. No. 1000121), following the manufacturers' protocol. All the libraries were sequenced in a PE150 mode (Pair-End for 150bp read) on the NovaSeq platform (Illumina).