title: mRNA display run 3, sample 16
design: The mutation library ORF containing randomized CDR2 and CDR3 was built by cycles of overlap extension PCR with Ultramers (IDT) followed with PCR purification. This library was further diversified by one-piece isothermal assembly using a linearized plasmid library backbone. After Gibson assembly into a plasmid backbone, the product was electro-transformed into cells for plasmid mini prep. The library underwent multiple rounds of mRNA display, with subjection to negative and positive selections against SARS-CoV-2 (Omicron) Spike RBD-coupled Magnetic Beads.