title: GSM6423819: D9 CARD9 R101C replicate 3, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Skin was harvested with sterile tools, cut into small pieces with scissors and incubated for 1.5h in digestion solution containing Liberase (Roche #385040) and DNAse I (Roche #10104159001) at 370C with agitation. After the digestion, samples were placed on ice and the reaction was stopped using stop buffer containing 5% FBS and 1mM EDTA. Cells were centrifuged  5min 1500 rpm at 40C and washed 1x with PBS. Cells were passed through a 100uM cell strainer and counted. Single cell suspensions were processed using the Chromium Single Cell 3' Gene Expression kit (v3.1, 10x Genomics) per manufacturer's instructions. Libraries were sequenced on the Illumina NovaSeq SP per manufacturer's instructions.