title: RNA-seq of ddm1-L2 1 h rep1
design: An Illumina Library Prep kit was used according to the manufacturers instructions. Then the double stranded PCR products were heat denatured to single strand, and then circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The final qualified libraries were sequenced by an DNBSEQ-T7 machine.