title: GSM8438868: NC, bio rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Normal murine bone marrow erythroid precursor cell or AEL murine bone marrow leukemic cells in each sample was sorted 10 x10^6 cells and extracted by RNeasy Mini Kit (QIAGEN). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.