title: GSM7520006: single cell mouse ckit rep2 R_loop; Mus musculus; OTHER
library_construction_protocol: All the cells were fixed by methanol first, and co-icubate with S9.6 antibody for 1 hour, followed by binding of nano body -Tn5 and activated by addition of Mg2+ For human cells, 1 million umbilical cord blood CD34+ positive cells were used as input for single cell R-loop mapping was performed. For mouse cells, 1 million c-Kit+ positive cells were used. Primary cells were firstly subjected to nuclear extraction with lysis buffer. Extracted nuclei were centrifugates and incubated with S9.6 containing antibody buffe. Unbound S9.6 was washed away with the same buffer followed by incubating cells with Dig300 washing buffer containing 5 mM nanobody Tn5 . Library preparation protocol was following the procedure of single cell nano-tag Tn5