title: GSM8438844: p53-/-; Myc Hepatoblasts, drug, 7days, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from cells using column-based methods with the NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer's instructions. The RNA Integrity Number (RIN) was assessed using the TapeStation instrument (Agilent Technologies). 1000 ng of total RNA was used as the starting material. RNA-seq library preparation was performed using the Illumina Stranded mRNA Prep, Ligation kit and Illumina RNA UD Indexes Set A (Illumina, Cat). The quality of the prepared libraries was assessed using the TapeStation instrument (Agilent Technologies).