title: GSM8369819: iETV2 EC expanded in hECSR Replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Tri Reagent and Direct-zol RNA Miniprep kit Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.