title: RNA seq of lung cells isolated from female adult C57Bl6 vaccinated subcutaneous BCG (1x10^6 cfu)
design: The NEBNext Ultra II Directional RNA Library Prep Kit and Poly(A) mRNA Magnetic Isolation Module were used to process 200 ng total RNA per sample according to the manufacturers instructions. Limited cycle PCR was performed with 100 ng for 14 cycles. Libraries to be multiplexed in the same run were pooled in equimolar quantities, calculated from Qubit and Bioanalyser fragment analysis.