title: Transcriptional Responses of Human Granulocytes to Candida albicans and Aspergillus fumigatus
design: Purified RNA was fragmented using RNAse III, and Ion adapters were ligated to the resulting fragments. Reverse transcription was conducted using the SuperScript III Enzyme Mix. Barcoded primers were utilized to amplify the libraries, employing the Platinum PCR High Fidelity polymerase. Ion Sphere particles and the Ion PI Hi-Q Chef Kit were then used for library template amplification on the Ion Chef instrument. These library templates were then loaded onto Ion PI Chips and sequenced on an Ion Proton Sequencer.