title: Deep-sequencing of SAMD00642736
library_construction_protocol: HEK293T cells were seeded on a 6-well-plate. After 24 h, the cells were co-transfected with HTLV-1, HTLV-2, or STLV-1 infectious clones in presence or absence of a vector expressing an hA3 family gene or sA3G. After 48 h, the transfected cells were seeded on 96-well-plate and co-cultured with CSCF-labeled Jurkat cells. After 48 h, Jurkat cells were collected by cell sorting (AriaIII). Then genomic DNA was extracted. To amplify the pX region of provirus sequences of HTLV-1, HTLV-2, and STLV-1, PCR was performed. The libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina), according to the manufacturer's instructions. Then deep-sequencing analysis was performed with MiSeq (Illumina). 