title: S. alvi amsE::KanR
design: Genomic DNA (gDNA) was purified using a DNeasy Blood & Tissue kit (Qiagen, Germany). For library preparation, 10 ng gDNA was input into the xGen DNA lib Prep EZ kit using the xGen Deceleration module (Integrated DNA Technologies, USA). All reactions were carried out at 20% of the manufacturers recommended volumes, with dual 6-bp indexes incorporated during a final 12 cycle PCR step. Sequencing was carried out at Psomagen (USA).