title: GSM8332756: Input wild type after injury replicate 3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: The homogenized heads were incubated with magnetic agarose GFP-Trap beads to pulldown ribosomes with associated mRNAs. RNA was extracted from ribosomes bound to the beads according to the NucleoSpin RNA (input) and RNA Plus XS (pulldowns) kit manufacturer's recommendations. Each sample was then processed in three technical replicates for RNA sequencing (RNA-seq). The library preparation was performed with poly-A purification for input samples and  skipped poly-A for the pulldowns, both without rRNA depletion.