title: WGS of Fungus F31
design: libraries were prepared using Oxford Nanopore Technologies (ONT) LigationSequencing Kit (SQK-LSK109) with NEBNext Companion Module (E7180L) in addition to Native Barcode Kits (EXP-NBD104, EXP-NBD114) to manufacturers specifications. All samples were runon Nanopore R9.4.1 flow cells and MinION Mk1B device. Post-sequencing, Guppy (v5.0.16) wasused for high accuracy base calling (HAC) and demultiplexing 20. Quality control and adapter trimming was performed with porechop. No size selection or shearing was performed during library prep and native DNA was sequenced