title: GSM8121150: 6DT1 shSmarcd1 #4 rep2 [RNA-seq spheroids]; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from cells 10 days after seededing. Each biological replicate was seeded from a different pasage of cells on subsequent days. Cells were collected and washed with PBS and then collected in 1ml TriPure isolation reagent, followed by organic extraction with chloroform and precipitation by isopropanol. RNA was then purified using the RNeasy Mini Kit (Qiagen) with on-column DNase digestion. RNA quality was tested using the Agilent 2200 TapeStation electrophoresis system, and samples with an RNA integrity number (RIN) score >7 were sent to the Sequencing Facility at Frederick National Laboratory Preparation of mRNA libraries and mRNA sequencing was performed by the Sequencing Facility using the HiSeq2500 instrument with Illumina TruSeq v4 chemistry.