title: GSM7074662: fulminant myocarditis patient#3; Homo sapiens; ncRNA-Seq
library_construction_protocol: Before eating/drinking in the morning, peripheral blood samples (8mL) were collected using an EDTA tube through a routine venepathesis procedure.A total of 4mL of plasma was collected from each participant, and sEVs were extracted by ultracentrifugation.Total RNA from the sEVs was extracted using the miRNeasy® Mini kit (Qiagen, cat217204). RNA concentration, purity, and integrity were assessed using the RNA Nano 6000 detection kit from the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA libraries were prepared for sequencing using standard Illumina protocols