title: GSM7782750: TC-32 WT, A, IgG, rep 1; Homo sapiens; OTHER
library_construction_protocol: CUT&RUN (14-1048, EpiCypher) was carried following manufacture's instructions with slight modifications. In brief, 5 x 105 cell/condition were harvested, washed, and bound to activated Concanavalin A (ConA) conjugated paramagnetic beads. The ConA beads–cell complex was resuspended in antibody buffer, and incubated with the appropriate antibody overnight at 4°C. Following incubation, pAG-MNase  was added to each CUT&RUN reaction to allow binding to the antibody-labelled chromatin. Targeted chromatin was digested and released by addition of CaCl2 (supplemented with S. cerevisiae spike-in, 29987L; Cell Signal Technology). Fragmented chromatin was purified using CUTANA Purification kit. Purified DNAs were quantified using Qubit fluorometer. The CUTANA CUT&RUN Library Prep kit (14-1001, EpiCypher) was used for library preparation following manufacturer's instructions