title: Short-read WGS of Candida albicans: SC5314 CRISPR-competent tlo-null lineage 1 a/alpha
design: -80C frozen stock was struck to solid YPD medium and incubated at 30C for 2 days. Cells from the densest part of the streak were then used to start an overnight culture in liquid YPD medium at 30C. The next day, ~10^8 cells were used to extract genomic DNA using the Zymogen Quick-DNA Fungal/Bacterial Miniprep Kit, after which they were sent to the Applied Microbiology Services Laboratory (AMSL, now IDI-GEMS) at The Ohio State University for processing. Libraries were constructed via tagmentation and dual-index barcoding using a modified protocol for the Illumina (L) Tagmentation kit (Illumina, cat# 20040537) to produce average final fragment sizes of approximately 450-500 bp. The libraries were sequenced for 2x150 paired-end reads on an Illumina NextSeq 2000. Reads were demultiplexed and Illumina adaptors were trimmed by AMSL.