title: Long read ONT WGS of Staphylococcus aureus mfd allele swap C222
design: Long-read S. aureus mfd allele swapped whole genome was sequenced on the MinION platform using R10.4.1 MinION flowcells (Oxford Nanopore Technology) with the Rapid Barcoding Kit (Oxford Nanopore Technology, SQK-RBK 114(?).96) according to the manufacturer's instructions using 50 ng of isolated gDNA. Base-calling was conducted with Dorado super accuracy mode using the 'dna_r10.4.1_e8.2_sup@v3.5.1.cfg' configuration (Oxford Nanopore Technology).