title: NGS sequencing of spacers acquired 2 hr post electroporation of DNA oligonucleotides containing a given protospacer. Acquired spacers are in the modified S. pyogenes type II-A CRISPR-Cas locus in S. aureus RN4220.
design: PCR of the CRISPR array, followed by amplicon size selection by PippinHT and illumina library preparation