title: RNA-seq of Metarhizium robertsii
design: After total RNA was extracted, mRNA was purified from total RNA (7ug) using Dynabeads Oligo (dT) (Thermo Fisher, CA, USA) with two rounds of purification. Following purification, the mRNA was fragmented into short fragments using divalent cations under elx_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_  x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_8_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_x_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_x_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_x_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_x_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000__xDC00__x0000__x0000_x_x0000__x0000_ _xDC00__x0000__x0000_x_x0000__x0000_ x_x0000__x0000_ x_x0000__x0000__x0000__x0000_x_x0000__x0000__x0000__x0000_ cycles of denaturation at 98 for 15 sec, annealing at 60 for 15 sec, and extension at 72 for 30 sec; and then final extension at 72 for 5 min. The average insert size for the final cDNA librarys were 30050 bp. At last, we performed the 2150bp paired-end sequencing (PE150) on an Illumina Novaseq 6000 following the vendor's recommended protocol.