title: Bacteria (16S) and Fungal (ITS) amplicon of Jornada Basin LTER surface soils
design: DNA extraction was performed with 0.25 g of composite soil using QIAGEN DNeasy PowerSoil kit (Qiagen, Germantown, MD, USA) following manufacture standard protocol. ITS1F and ITS2 primers set were used to amplify the internal transcribed spacer region (ITS) for fungal community according to Smith and Peays Illumina Miseq protocol (Smith and Peay, 2014). 515F and 806R primers set were used to amplify 16S V4 region for bacterial community following (Caporaso et al., 2011). PCR was run in triplicates per sample and then pooled and cleaned using using NucleoSpin Gel and PCR Clean-up kit (Macherey- Nagel, Hoerdt, France). All cleaned PCR products were pooled to produce equimolar library mixture.