title: RNA-Seq of processionary caterpillar Ochrogaster lunifer setae fields
design: Three late-instar caterpillars were dissected in PBS and the regions of cuticle underlying the defensive setae transferred to RNAlater for storage at 80C. Total RNA (2.0 g) was extracted using Trizol according to the supplier's instructions, then submitted to Institute for Molecular Bioscience Sequencing Facility at The University of Queensland. TruSeq libraries with 150 bp inserts were constructed and sequenced in paired-end format according to the manufacturer's instructions using a HiSeq 2500 instrument (Illumina, San Diego, CA, USA). Paired-end reads (6,340,2000) were obtained and assembled de novo using an automated pipeline for trimming, correcting and assembling RNA-Seq reads using fqtrim (https://github.com/gpertea/fqtrim), prinseq (Schmieder & Edwards, 2011) and Trinity 2.4.0 (Haas et al., 2013). The transcriptome was annotated by BLASTx against the National Centre for Biotechnology Information (NCBI) non-redundant protein database, and the transcript abundance in transcripts per million (TPM) was estimated using the RSEM algorithm.