title: Exome sequence of human: Sample SM-CEOMY
design: Whole-exome sequencing (WES) was performed at the Broad Institute. DNA was plated at a concentration of 2ng/uL and volume of 50uL into matrix tubes, which allowed for positive barcode tracking throughout processing. Samples were sheared using a Broad developed protocol optimized for ~180bp size distribution. Library construction was performed using the KAPA Library Prep kit, with palindromic forked adapters from Integrated DNA Technologies. Libraries were pooled prior to hybridization. Hybridization and capture were performed using the relevant components of Illumina's Rapid Capture Enrichment kit, with a 37Mb target. All library construction, hybridization, and capture steps were automated on the Agilent Bravo liquid handling system. After post-capture enrichment, library pools were quantified using qPCR, normalized to 2nM, and denatured using 0.1N NaOH on the Hamilton Starlet. Flowcell cluster amplification and sequencing were performed according to the manufacturer's protocols (Illumina) on either the HiSeq 2000 v3 or HiSeq 2500 runs, and utilized sequencing-by-synthesis kits to produce 76bp paired reads. The target coverage was 150x mean target coverage per each sample. Output from Illumina software was processed by the Picard data-processing pipeline to yield BAM files containing de-multiplexed, aggregated aligned reads.