title: ="UCE sequences of  "&B190
design: DNA was extracted from single ants, either adults or pupae, using the DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA), and quantified with a Qubit fluorometer (HS Assay Kit, Life Technologies Inc., Carlsbad, CA). We sheared 5-50 ng input DNA to a target size of ~600 bp using either a Diagenode BioRuptor (Diagenode Inc., Denville, NJ) or QSonica Q800R3-110 (Qsonica Inc., Newtown, CT). This product served as input for a modified genomic DNA library preparation protocol (Kapa Hyper Prep Library Kit, Kapa Biosystems) that included SPRI bead cleanup using a generic substitute (Rohland & Reich, 2012) and custom dual-indexing barcodes (Glenn et al., 2016). For UCE enrichment, 9-10 libraries were combined at equimolar ratios and adjusted to a final pool concentration of 147 ng/ml. Each pool was enriched using a set of custom-designed probes, hym-v2-ants (MYcroarray, Inc., now ArborBiosciences, Ann Arbor, MI) targeting 2,524 UCE loci in ants (Branstetter et al. 2017), following a standardized in-solution protocol (Blaimer et al. 2018; Ward & Branstetter 2017). The DNA concentration of enriched pools was quantified with qPCR, and these values were used to make an equimolar pool-of-pools, which was sequenced either an Illumina HiSeq 2500 at the University of Utah Huntsman Cancer Center or carried out by RAPiD Genomics (Gainesville, FL) on an Illumina Novaseq 6000.