title: Illumina Genome Analyzer IIx sequencing of SAMD00078872
library_construction_protocol: Ribosomal RNAs were depleted from total RNA samples by using mRNA-ONLYÃÂÃÂ¢ÃÂÃÂÃÂÃÂ¢ Prokaryotic mRNA Isolation Kit (epicentre). The depletion of rRNAs was confirmed by analysis with Agilent 2100 Bioanalyzer with RNA 6000 pico kit. The sample was then treated with tobacco acid pyrophosphate (TAP) (Epicentre) to generate 5' -mono-phosphate for linker ligation followed by ligation with RNA adapter (5'ACACUCUUUCCCUACACGACGCUCUUCCGAUCUGACU-3'). All the steps were performed according to manufacturer's instruction. cDNA was then synthesized  using random primer (5'-CAAGCAGAAGACGGCATACGAGCTCTTCCGATCTNNN NN-3') and Super script II Reverse Transcriptase (Invitrogen) as previously reported (Chumsakul et al., 2011). cDNA ligated with adapter were then enriched by amplification for 14 cycles using primer 1.1 and 2.1 (Illumina) and iProof high-fidelity DNA polymerase (Bio-Rad) according to the condition described in the protocol "Preparing Samples for Sequencing Genomic DNA" (Illumina). Single end sequencing with 75 bp read was carried out on the GAIIx plateform (Illumina).