title: NGS of fecal sample of human: S001-A1.A2.before
design: V3-V4 region of 16S rRNA was amplified with PCR. 5 ng of total DNA was used as PCR template. Universial primers targeting regions of the 16S rRNA(341F and 805R) were used. PCR products were confirmed with gel electrophoresis and purified using AMPure XP beads (Beckman Coulter, USA). 16S rRNA library was prepared using the Nextera XT Index Kit (Cat FC 131 1001, Illumina, USA). Quantification of PCR amplicons were conducted using Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific, Wilmington, DE, USA). Size and integrity of PCR products were checked using 4200 TapeStation System (Agilent, USA).