title: GSM8333155: HES1-IGG1_2; Homo sapiens; OTHER
library_construction_protocol: In summary, 60,000 ADSCs were collected, rinsed, and subsequently exposed to Concanavalin A-coated magnetic beads. Subsequently, the cells were treated with either an anti-flag antibody (Cat. 14793S, Cell Signaling Technology, USA) or an isotype control antibody (Cat. 2729S, Cell Signaling Technology, USA) for a duration of 2 hours, followed by an additional hour with a secondary antibody at ambient temperature. The hyperactive pG-Tn5 transposonase was then introduced to facilitate tagmentation. The reaction was terminated, and DNA fragments were extracted by PCI. DNA fragments were extracted by PCI, followed by PCR amplification using indexed P5 and P7 primers. The library products were enriched, quantified and finally sequenced on a Novaseq 6000 sequencer (Illumina) with PE150 model.