title: GSM7830306: liver_SHAM-4; Mus musculus; ATAC-seq
library_construction_protocol: nuclei were extracted from the liver tissues (Demonstrated Protocol-Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing) and 100k nuclei were mixed with 2x reaction buffer and Tn5 Transposase in a total volume of 50 µL, and incubated for 30-45 min at 37°C. Transposed DNA was purified in 11 µL elution buffer according to the manufacturer's protocol by using the Monarch PCR&DNA Cleanup kit (Biolabs) Transposed DNA was purified in 11 µL elution buffer according to the manufacturer's protocol by using the Monarch PCR&DNA Cleanup kit (Biolabs). Afterwards, transposed DNA was amplified according to published protocol and PCR primers95. Next, PCR-amplified DNA fragments between 150bp and 800bp were selected from the library by using SPRIselect beads (Beckman coulter) according to manufacturer's instructions. The library quality was checked with the 2100 Bioanalyzer (Agilent) using the high sensitivity DNA kit (Agilent)