title: ddRAD-Seq of Halophila stipulacea: adult plant
design: Genomic DNA was extracted from frozen samples using a cetyltrimethyl ammonium bromide (CTAB) chloroform/isoamyl alcohol (24:1) isolation protocol. To build the ddRAD-seq library DNA was digested with the restriction enzymes SbfI and NlaIII, and barcodes/adaptors were ligated to the sheared ends. Fragments were size-selected by agarose gel targeting an insert size between 400 and 700 bp. Selected fragments were purified with MinElute agarose gel extraction kit, amplified in PCR reactions, purified using a column MinElute PCR purification kit and AMPure XP beads. The library was sequenced on a single lane of an Illumina MiSeq.