title: GSM7159146: HeLa_CRISPR_ESRRB_2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using TRIzol for the construction of sequencing libraries. 1.Sample QC. 2. mRNA isolation. 3. mRNA Fragmentation. 4. cDNA synthesis. 5. End Repair, Add A and Adaptor Ligation. 6. PCR. 7. Library QC. 8. Circularization 9. Sequencing