title: CH340-16SrRNA
design: Microbial DNA was extracted using an improved version of the repeated bead-beating plus column method. Then, V3-V4 hypervariable regions of the 16S rRNA gene were amplified by using the 341F and 785R primers for the library preparation step. The final libraries, indexed and purified, were sequenced on an Illumina MiSeq platform with a 2  250 bp paired-end protocol, following the manufacturer's instructions