title: snRNA-Seq of mus musculus: 10 month striatum of zQ175 - 4 month DBD control
design: Isolated nuclei were counted using Luna FX7 (Logos Biosystems) automated cell counter with AO/PI to adapt the volume of the nuclei suspension targeting 10.000 nuclei for each sample. The nuclei suspension was subjected to the 10x Genomics Single Cell 3 Reagent Kit v3.1 workflow and library preparation was performed according to the manufacturers instructions (10X genomics protocol: CG000315). cDNA and library quality were assessed using Qubit and BioAnalyzer technology. The final libraries were pooled in an equimolar ratio and submitted to CeGaT (Tbingen) for sequencing using Illumina NovaSeq6000 S4 platform targeting at least 20.000 reads per nucleus.