title: GSM7074843: K562 CEBPA reporter cell line, nontargeting sgRNA ribo RNAseI, biol rep 1; Homo sapiens; OTHER
library_construction_protocol: 30ug of RNA for ribosome profiling was harvested by TRIzol extraction; 1ug of RNA for RNA-seq was harvested by phenol-chloroform extraction. Ribosome footprint libraries were prepared by PCR amplifcation from circularized cDNA using NEBNext Multiplex oligos; matched RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit following the manufacturer's protocol.