title: GSM6435355: snRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: For scRNA-seq, freshly excised tissue was rinsed with RPMI 1640 medium (Thermo Fisher Scientific, Inc.) and cut into 1-2-mm pieces. The samples were incubated at 37°C for 40 min with digestive solution, containing 0.25% trypsin (Thermo Fisher Scientific, Inc.) and 10 μg/ml DNase I (Sigma-Aldrich; Merck KGaA) dissolved in 5% FBS (Thermo Fisher Scientific, Inc.). Samples were manually oscillated every 5 min, and filtered twice using a 40-μm nylon mesh (Thermo Fisher Scientific, Inc.). Erythrocytes were removed using 1X Red Blood Cell Lysis Solution (Thermo Fisher Scientific, Inc.).