title: U937-RNA-seq
design: The NEBNext UltraTM RNA Library Prep Kit for Illumina is used to construct the library. The library construction process involves using fragmented RNA as a template and random oligonucleotides as primers to synthesize the first strand cDNA using M-MuLV reverse transcriptase. The RNA strand is then degraded using RNaseH, and in an ONA polymerase I system, the second strand cDNA is synthesized using dNTPs as substrates. The purified double-stranded cDNA is subjected to end repair, A-tailing, and sequencing adapter ligation. The cDNA fragments of approximately 250-300bp are selected using AMPure XP beads, followed by PCR amplification and purification of the PCR products using AMPure XP beads again. Finally, the library is obtained.