title: NextSeq 2000 paired end sequencing; H3K4me3 HiChIP for Paclitaxel resistant L3.6pl pancreatic cancer  cells (Non targeting vs siJunD)
design: H3K4me3 HiChIP for Paclitaxel resistant L3.6pl pancreatic cancer  cells (Non targeting vs siJunD)
library_construction_protocol: Cells (24 hours of knockdown) were washed with PBS and trypsinized. Cells were fixed for 10 minutes with 1% Formaldehyde and suspended in ice-cold Hi-C Lysis Buffer (10mM Tris-HCL pH 7.5, 10mM NaCl, 0.2% NP-40 in water). Streptavidin-C1 beads (Thermo Fisher) were used to capture biotinylated DNA Library preparation using the MicroPlex Library Preparation Kit (Diagenode)