title: RNA-Seq of bermudagrass: leaf blade protoplast cells 3
design: Total RNA was extracted from the isolated protoplasts using RNAprep pure Plant kit (Tiangen, Beijing, China). RNA integrity and concentration were determined by gel electrophoresis and Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, USA), respectively. cDNA libraries were prepared using Illumina TruSeq Sample Preparation Kit (Illumina, San Diego, USA) according to the manufacturers instructions. Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, USA) was used to check the quality of the libraries. The qualified cDNA libraries were paired-end sequenced using Illumina HiSeqTM 3000 (Illumina).