title: 18S rRNA of Cephalothrix simula gut metagenome  sample 20
design: C. cf. simula samples were dissected into 20 mg cross sections. Total genomic DNA was extracted from each sample using an E.Z.N.A. Mollusc DNA Kit (Omega Bio-tek, USA). A 18S rDNA metabarcoding approach was used to investigate the biotic diversity in C. cf. simula diet. Universal eukaryotic primers were used to target the V9 variable region of the 18S rDNA. The V9 18S rRNA amplicon library preparation and Illumina NovaSeq 6000 paired-end sequencing (2x250 bp)