title: NGS SYK-6 RB-TnSeq library: Wx + 10 mM vanillate enrichment Replicate C
design: A 1 mL cell pellet was used to extract and purify gDNA. Specific primers amplified the transposon-associated barcode region with Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA). Samples were pooled and sent to Genewiz for sequencing.