title: RNA-Seq of mus musculus:adult male brain
design: Brain tissues were frozen in an isopentane bath at -40C and transferred to -80C for storage.Frozen tissues were embedded in pre-cooled OCT and frozen sections were taken at 10 m thickness. Sequencing libraries were constructed using the Kapa HIFI HotStart ReadyMix PCR KitKAPA Biosystems and sequenced using a Novaseq6000 sequencer in pair-ended 150150 mode