title: Nanopore MinION sequencing of S. enterica HS-180Salm isolated from harvested oysters
design: Single colony grown 24h at 35C in TSB broth, 0.5 mL of culture pelleted and resuspended in Zymo DNA/RNA Shield. DNA extraction using Zymo Quick-HMW kit with RNAseA treatment. Rapid barcoding sequencing kit (SQK-RBK-004) and 1D MinION sequencing using FLO-MIN106 R9.4 flowcell