title: RNA-Seq of Mauremys reevesii: adult mitochondrial genome
design: One adult turtle (M. reevesii) was selected to extract muscle for isolation of mitochondrion by using Mitochondria Isolation Kit (Thermo), then mitochondrial RNA was extracted with Trizol Universal Reagent (Tiangen), the agarose gel electrophoretic analysis showed degradation of the RNA, which was eluted with nuclease-free H2O. Then, the qualified mitochondrial RNA was subjected to single-ended 50bp sequencing mode of Illumina Hiseq 2500 platform.