title: Heiss island soil metagenome 22
design: DNA from frozen soil samples was isolated using the DNeasy PowerSoil Pro Kit(Qiagen, Germany). Two nanograms of DNA were used as aninput for the library preparation. The amplicon libraries of the hypervariable V4 region ofthe 16S rRNA gene were prepared using the two-stage PCR strategy with the primers515F and Pro-mod-805R. PCR of every DNA sample was carried out induplicates, the detailed amplification protocol is described previously