title: Targeted Sequencing; Tasmanian Devil; Microchip 982009104249879
design: To effectively sample across the genome, a target-capture panel of ~197k probes was designed. We extracted DNA from devil and tumor tissues and sheared DNA samples to a modal fragment size of ~200250 bp prior to library preparation. We performed library preparation using the Twist Mechanical Fragmentation and Twist Universal Adapter System (Twist Bioscience). Libraries were multiplexed into groups of eight and enriched for our target capture panel using the Twist Target Enrichment Protocol. We used salmon sperm DNA (Invitrogen) as a blocking. Libraries were further multiplexed into sequencing pools containing 24 capture reactions each (192 total libraries per sequencing pool).