title: WGS of M.brumae
design: Genomic DNA (2g) was diluted in 200L of elution buffer and fragmented using g-TUBE (Covaris) by centrifuging at 2400g for 2min. The fragmented DNA was concentrated using AMpure PB magnetic beads (Beckman Coulter) and used for the preparation of a single non-multiplexed sequencing library. A ready-to-sequence SMRT bell-Polymerase Complex was created using PacBios Sequel binding kit 3.0 according to the manufacturers instructions. The final library was sequenced on 1 Sequel SMRT Cell 1M v3, taking a 10h movie using the Sequel Sequencing Kit 3.0.