title: RNA-Seq of basil leaf -FR Mid
design: Total RNA was extracted from leaf tissues of basil using an RNAeasy plant mini kit (Qiagen). Total RNA was extracted from the top-most fully-expanded single leaves from at least five biological replicates for each environmental condition and was pooled into one sample for each condition. Prior to sequencing, sample quantity and quality was measured using a Qubit fluorometer (Thermo Fisher Scientific). mRNA was obtained from the total RNA pool before fragmentation, cDNA synthesis, adapter ligation and size selection; and the size-selected ligated DNA products were then amplified by PCR to produce a sequence-ready library.