title: Inlet point
design: The raw data quality was checked using FastQC  and MultiQC  software. The raw reads were trimmed (20bp) from 5 end to remove the degenerate primers. The trimmed reads were processed to remove adapter sequences and low quality bases (Q < 20)) using Trimgalore v0.6.7 with default parameters. The QC passed reads were imported into mothur v1.46.1 and the pairs were aligned with each other to form contigs.