title: RNA-seq of listeria monocytogenes overexpress LmHU
design: Fresh bacterial cultures of each strain were inoculated into 50 mL BHI medium at 1% and cultured to the mid-logarithmic phase (OD600=0.4). Each strain was carried out in three biological replicates. Bacteria cells were harvested and used to extract the total RNA using TRIzol Reagent according to the manufacturers instructions (Invitrogen). High-quality RNA sample (OD260/280 = 1.8~2.0, OD260/230  2.0, RIN  6.5, 28S:18S  1.0) was used for construct sequencing library.  Whole mRNAseq libraries were generated by Guangdong Magigene Biotechnology Co.,Ltd. (Guangzhou, China) using NEB Next UltraTM Directional RNA Library Prep Kit for Illumina (New England Biolabs, MA, USA) following the manufacturers recommendations. Library quality inspection was required. PE150 sequencing would be performed on Illumina high-throughput sequencing for libraries qualified for quality inspection. The original image data files obtained by sequencing were converted into Raw Reads through Base Calling analysis.