title: Shotgun metagenomic sequence from pig feces
design: Metagenomic libraries were prepared with KAPA HyperPlus kits (Roche Diagnostics, Indiana, USA) and automated on EpMotion automated liquid handlers (Eppendorf, Hamburg, Germany). Libraries were prepared following manufacturer's instructions, except amplified libraries were pooled equi-volume and batch-cleaned with sparQ PureMag Beads (Quantabio, Massachusetts, USA). Two successive rounds of 0.8X cleanings were performed, with each round including two ethanol washes. Sequencing was performed on the Illumina NovaSeq 6000 sequencing platform at the University of California San Diego Health Sciences Microbiome Core with paired-end 150 bp reads.