design: 16,500 cells were run on the 10X Chromium to partition single cells into droplets containing uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 5' NextGEM v2 chemistry. Amplified full-length cDNA from poly-adenylated mRNA is used to enrich full-length V(D)J segments (10x Barcoded) via PCR amplification with primers specific to the BCR constant region. BCR transcripts were amplified in from the same amplified cDNA material. Enzymatic fragmentation and size selection are used to generate variable length fragments that collectively span the V(D)J segments of the amplified BCR transcripts prior to library construction.
library_construction_protocol: UMIGS-Maryland-Genomics Illumina Construction Protocol Details