title: GSM7999256: Zebrafish Transcriptome - mecp2 Mutant 2; Danio rerio; RNA-Seq
library_construction_protocol: Embryos derived from incrosses of mecp2-Q63X homozygous adults or wild-type adults were pooled in groups of three and processed for RNA extraction at 4 dpf as previously described in Yoon et al 2022. cDNA libraries were prepared using the TruSeq stranded mRNA library preparation protocol with poly-A selection and sequenced on the Illumina HiSeq. 2500. 10 biological replicates of each genotype, confirmed from raw sequencing data alignments using the Integrative Genomics Viewer, were analyzed.