title: GSM6964552: Enterocyte_Sham; Rattus norvegicus; RNA-Seq
library_construction_protocol: Nuclei were isolated from 9 flash-frozen rat colon tissue samples using a proprietary extraction method (Singulomics, Bronx, NY). Nuclei were captured using 10X Genomics Chromium manufacturer's protocol (10X Genomics, Pleasanton, CA). Captured nuclei were used to construct 3' single cell gene expression libraries (Next GEM v3.1) using the 10x Genomics Chromium system (10x Genomics, Pleasanton, CA). Libraries were seque­nced with ~150 million read pairs (PE150) per sample on an Illumina NovaSeq sequencing system (Illumina, San Diego, CA). Manufacturer's instruction were used for library construction