title: GadR_Non_AD_2rep_RNA-seq
design: ribosomal RNA was removed from total RNA with the Illumina Illumina Ribo-Zero Plus rRNA Depletion Kit. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, PCR amplification, and purification with AMPure XP beads. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.