title: GSM7234197: Day4 (HH24) Chick Trunk_puck25_Slide-seq; Gallus gallus; RNA-Seq
library_construction_protocol: To determine the spatial gene expression within chick trunk tissue at the developmental  stage prior to the dorsal migration of the primary SG and axon extensions from the  spinal-cord based PGNs, we harvested day 4 (HH24) unlabeled chick embryos. Fresh  frozen HH24 trunk tissues were transversely cryo-sectioned to approximate single cell  thickness (10um) and carefully placed on a single sequenced Slide-seq puck containing  ~74,000 uniquely barcoded 10um beads. Tissue was then lysed, and the poly-A mRNA  captured by poly(dT). Tissue was then lysed, and the poly-A mRNA  captured by poly(dT) for downstream cDNA synthesis, NGS library construction, and  Illumina sequencing on a NextSeq 500 machine using the following paired end length:  44 bp Read1, 8 bp Index and 50 bp Read2.