title: GSM7918290: Lin_2_Chip_Seq_for_Nynrin; Mus musculus; ChIP-Seq
library_construction_protocol: Lineage-negative cells of BM from  3×Flag-Nynrin-Tg mice were fixed with 1% formaldehyde for 10min.Then cells were demobilized and lysed. ChIP-seq libraries were prepared using TruePrepTM DNA Library Prep Kit V2  from  Vazyme. 10ng of DNA was used as starting material for input and IP samples.Libraries were amplified using 16 cycles on thermocycler.Post amplification libraries were size selected at  100-500bp in length using Agencourt AMPure XP beads from Beckman Coulter.Libraries were validated using Agilent High Sensitivity DNA kit