title: GSM8332962: HT1080, HA, ChIP, rep 2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 30 minutes. After quenching and cell lysis, chromatin was sonicated using an M220 focused-ultrasonicator (Covaris). Libraries were prepared for sequencing using standard Illumina protocols. The final DNA library was obtained by size selection and PCR amplification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.