title: RNA-seq on four Aedes aegypti lines differing in resistance to deltamethrin
design: RNA-seq on four Aedes aegypti lines differing in resistance to deltamethrin
library_construction_protocol: For each line, four RNA-seq libraries were prepared from four batches of 25 calibrated three-day-old non-blood-fed females not exposed to insecticide.  Total RNA was extracted using TRIzol® (Thermo Fisher Scientific) following manufacturer's instructions. RNA samples were then treated with RNase-free DNase set (Qiagen) to remove gDNA contamination, purified on RNeasy mini columns (Qiagen) and QC checked using Qubit (Thermo Fisher Scientific) and bioanalyzer (Agilent).  RNA-seq libraries were prepared from 500 ng total RNA using the NEBNext® Ultra™ II directional RNA library Prep Kit for Illumina (New England Biolabs) following manufacturer's instructions. Briefly, mRNAs were captured using oligodT magnetic beads and fragmented before being reverse transcribed using random primers. Double-stranded cDNAs were synthesised, end-repaired, and adaptors were incorporated at both ends. Libraries were then amplified by PCR for 10 cycles and purified before QC check using Qubit and Bioanalyzer.