title: GSM6435458: Mouse forelimb motor neurons - RNA-seq 2 [Limb_MN-7]; Mus musculus; RNA-Seq
library_construction_protocol: Spinal cords of Hb9-GFP (Jackson laboratory) were dissociated and GFP+ MNs were manually collected. The regions for forelimb MNs were visualized under a fluorescence dissection scope and separated from other neurons using a sharp scalpel. The spinal cords were digested with pronase for 30min at RT. The sequencing library was prepared using the TruSeq RNA sample prep Kit (Illumina, CA).