title: NEBNext Ultra II RNA library from Arabidopsis thaliana RNA (6tae, RNA was extracted from 3-5 mm long leaves from day-old juvenile tae plants obtained after 7 backcrosses of tae to Bla) sequenced on Illumina NextSeq 550 with NextSeq 500/550 High Output Kit v2.5.
design: RNA from leaves was extracted using RNeasy Plant Mini kit (Qiagen, Netherlands) according to the manufacturers protocol. RNA quality was checked using a Bioanalyzer 2100 (Agilent, United States) with a RNA 6000 Pico chip (Agilent, USA). Samples with RIN > 7 were employed for subsequent analysis. The library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA). The Illumina sequencing data was filtered for low read quality and adaptor trimmed with AdapterRemoval v2 (--minlength 50).