title: DNA-seq of soil fungi
design: Total DNA was extracted from soil using PowerSoil DNA isolation kit (Qiagen, Crawley, UK). Prokaryotic 16S rDNA region was amplified with primer 515F/806R (Wasimuddin et al., 2020), and fungal ITS2 fragment was amplified with primer gITS7/ITS4 (Ihrmark et al., 2012).