title: The immediate effect of high-intensity green LED stimulation on the gene expression profile of S. coelicolor A3(2)
design: The immediate effect of high-intensity green LED stimulation on the gene expression profile of S. coelicolor A3(2)
library_construction_protocol: S. coelicolor A3(2) was grown on MR5 plates for 24 h, then the substrate mycelia were harvested from agar by gentle scraping. Total RNA of S. coelicolor A3(2) was isolated from cells (approximately 100 mg wet weight) with Sepasol-RNA I super G reagent (Nacalai Tesque, Inc.). After treatment with RNase-free recombinant DNase I (TaKaRa Bio, Inc., Shiga, Japan), each sample was further purified using a High Pure RNA Isolation Kit (Roche Life Science, Penzberg, Germany). High-purity total RNA samples were sent to Bioengineering Lab. Co., Ltd. (Kanagawa, Japan) for the rRNA depletion using riboPOOLS (siTOOLs Biotech, Germany), and library construction using the RNA Directional Library Prep Set (MGI Tech, China).