title: 16S rRNA sequence of mus musculus: adult male feces
design: bacterial 16S rRNA genes were amplified using primers that span the V3-V4 regions: 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). Amplifications were conducted on an ABI GeneAmp 9700 PCR thermocycler (ABI, CA, USA), followed by purification, pooling and sequencing of amplicons using the Illumina MiSeq platform (Illumina, San Diego, CA, USA)