title: GSM6964591: WT2; Mus musculus; OTHER
library_construction_protocol: the brain tissues were prepared by permeabilization buffer and treated by 2 × nuclear run on buffer, then RNA was extracted by Triozol-LS. Biotin RNA was enriched by prewashed Dynabeads™ M-280 Streptavidin beads (Invitrogen, #11206D) The extracted RNA was used to perform reverse transcription, PCR amplification aaccording previously published protocol (Mahat et al., Nature Protocols, 2016).