title: RNA-Seq of Arabidopsis thaliana: flower bud
design: Total RNA was isolated from 200mg of young buds (anther stage 8-11) of athmgb15-4 mutant using RNASure Mini Kit (Nucleopore-Genetix).  NEBNext Ultra II Directional RNA library Prep Kit was used to prepare high quality libraries, according to manufacturers protocols and paired end sequencing reads of read length 151bp was generated with Ilumina HiSeq-X seqeuncing platform. Raw reads were checked individually by using FASTQC. Quality control and per-processing of FASTQ files are essential to providing clean data for downstream analysis.