title: GSM7074837: K562 CEBPA reporter cell line, Cancer and Apoptosis Sublibrary, Near Right bin; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was harvested with DNeasy Blood and Tissue kit (Qiagen, 69504). sgRNA fragments from genomic DNA were isolated by SbfI (New England Biolabs) restriction digestion and Ampure bead size selection then amplified by PCR for deep sequencing using custom primers.