title: Illumina NovaSeq 6000 paired end sequencing; 16s rRNA gene sequencing_young and old mouse gut microbiota_Stroke
design: 16s rRNA gene sequencing_young and old mouse gut microbiota_Stroke
library_construction_protocol: Fresh fecal pellets collected within 30 min after bowel movement from old, young, young_yFMT, young_oFMT and antibiotic groups. Mouse fecal bacterial DNA was extracted with Power Lyzer Power soil DNA isolation kit (catalog number: 12855-100, QIAGEN, Germantown, MD, USA)  Library was created by primers (341F/805R) designed to target the V3 and V4 regions of 16S rDNA, generate an amplicon about 465 bp in size.