title: GSM7794627: CD4T, WT PRO rep3; Mus musculus; OTHER
library_construction_protocol: PRO-seq was performed using the protocol from Mahat et al., 2016 with small modifications. Permeabilized cells were incubated in polymerase run-on reactions with ribonucleotide triphosphate (NTP) substrates. cDNA is amplified through 15–17 cycles of PCR amplification with Illumina TRU-seq multiplexing primers. Libraries were paired-end sequenced on Illumina HiSeq X ten platform.