title: cpDNA sequence of Ocimum africanum
design: Inqaba Biotech Genomic DNA samples were fragmented using an enzymatic approach (NEBNext Ultra II FS DNA Library Prep Kit for Illumina) Resulting DNA fragments were size selected (>200 bp), using AMPure XP beads. The fragments were end repaired and Illumina specific adapter sequences were ligated to each fragment. Each sample was individually indexed, and a second size selection step was performed. Samples were then quantified, using a fluorometric method (NEBNext Library Quant Kit for Illumina), diluted to a standard concentration (4nM) and then sequenced on Illuminas NextSeq500 platform, using a NextSeq mid out kit (300 cycle), following a standard protocol as described by the manufacturer. 200 Mb of data (2x150bp paired-end reads) were produced for each sample.