title: 16S V3-V4 of CoV48
design: Microbial DNA was extracted using an improved version of the repeated bead-beating plus column method(Turroni et al., 2017; Yu & Morrison, 2004). The V3-V4 hypervariable regions of the 16S rRNA gene were then amplified using the 341F and 785R primers(DAmico et al., 2019) for the library preparation step. The final libraries, indexed and purified, were sequenced on an Illumina MiSeq platform using a 2  250 bp paired-end protocol according to the manufacturers instructions