title: Genomic Sequencing of both Escherichia coli and Enterococcus: recurrent urinary tract infections
design: E. coli pellets followed the Qiagen blood and tissue kit instructions. E. faecalis pellets followed a protocol adapted from Huo, et al. 2015. Illumina sequencing libraries were prepared using the tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10bp unique dual indices (UDI) with a target insert size of 320 bp. No additional DNA fragmentation or size selection steps were performed. Sequencing was performed on an Illumina NovaSeq 6000 sequencer, producing 2x151bp paired-end reads.