title: RNA-Seq of HepG2 cells, grown on collagen, treated with SkQ1, replicate 1
design: Hepatocarcinoma HepG2 cells were plated on standard plastic adherent cell culture plates and on plates precoated with 10% rat collagen type I. Cells were treated with the mitochondria-targeted antioxidant SkQ1 (40 nM, 72 hours) and changes in overall morphology and actin cytoskeleton were analyzed. To measure the adhesion of HepG2 cells to collagen, cells grown in the presence or absence of SkQ1 were harvested, washed, and plated on collagen-coated plates for 40 min