title: Binary Alignment Map of Homo sapiens: Affected Patient with parathyroid cancer(Disease stage:pT1NoMo)
design: DNA is fragmented by acoustic disruption using Covaris S220 and then undergoes end repair, dA-tailing, adapter ligation and purification. The purified DNA is further selected for the right size before PCR amplification for library construction.Quantification and dilution of the library is performed using Qubit3.0.Then Agilent 2100 was used to determine the insert size and nucleic acid concentration.Effective concentration is determined by qPCR