title: RNA-Seq of the D425 human medulloblastoma cell line with knockout of AAVS1 negative control
design: This RNA library preparation method takes total purified RNA as input and enriches for polyadenylated (polyA+) transcripts using oligo-dT coated beads. PolyA+ RNA is subsequently fragmented using heat and Mg+ and reverse transcribed into cDNA using random priming. Illiumina adapters are ligated to dsDNA and PCR amplified.  Library preparation is done with the Roche KAPA mRNA Hyper Prep kit.