title: Acinetobacter
design: DNA fragments were end polished, A-tailed, and ligated using the NEBNext adapter for Illumina sequencing and further enriched by PCR with P5 and indexed P7 oligos. PCR products were purified using Agencourt AMPure XP beads (Beckman Coulter, Inc). The resulting libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and quantified using real-time PCR (to meet the criteria of 3 nM). The samples were sequenced and paired-end reads (150 bp) were generated on the instrument NovaSeq 6000.