title: GSM6659266: mESC_Firre_Rescue_firre_induced_48h_input_ChIPseq; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a Covaris M220 focused-ultrasonicator. ChIP-seq libraries were prepared using the KAPA HyperPlus Library Preparation Kit. 6.5 ng of DNA was used as starting material for input and IP samples. Libraries were amplified using 13 cycles on the thermocycler. Libraries were validated using the Agilent High Sensitivity DNA Kit.