title: Museum genomics of the Colorado potato beetle
design: All DNA extractions were conducted in an ancient DNA laboratory facility provided by the University of Wisconsin-Madison Biotechnology Centers Molecular Archaeology lab. A new Qiagen Blood and Tissue Kit was used to extract DNA from museum beetles individually. To remove beetles from pins, each dry pinned beetle was rehydrated in warm water for 10 minutes and then removed from the insect pin. The DNA extraction procedure follows the manufacturers protocol with the following modifications: the entire body of each beetle was soaked in lysis buffer and proteinase K for 24 hours at 56 degrees Celsius, and during elution, 50 uL of buffer AE was used in the first elution step and then passed through the spin column a second time. Each sample was prepared for Illumina short-read shotgun sequencing by the UW Biotech Center, following standard protocols modified for ancient insect DNA, and then combined in equimolar amounts for paired-end sequencing (2x150 bp) on the Novoseq 6000.