title: GSM7964826: Blimp1 KO rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA purification was performed using the Qiagen RNeasy Micro Plus Kit. Libraries were generated using the SMART-Seq v4 Ultra Low Input RNA kit followed by Nextera FLEX using UDI set B indexes. Samples were sequenced using NovaSeq 6000 or NextSeq 2000 flowcells to obtain 20M 2x75 bp reads. Some samples were sequenced as 2x101 bp reads. Cells were collected via FACS, washed twice in PBS + 1% FCS, resuspended in 350 ul RLT Plus Buffer (Qiagen) with 1% 2-Me, and frozen at -80 ⁰C. RNA purification was performed using the Qiagen RNeasy Micro Plus Kit. Libraries were generated using the SMART-Seq v4 Ultra Low Input RNA kit followed by Nextera FLEX using UDI set B indexes. Samples were sequenced using NovaSeq 6000 or NextSeq 2000 flowcells to obtain 20M 2x75 bp reads. Some samples were sequenced as 2x101 bp reads.