title: DNA-Seq of Mycoplasma hominis MHO45 strain
design: Genomic DNA extraction was performed using the Invitrogen PureLink Genomic Mini Kit according to the manufacturer's instructions. To prepare DNA libraries for sequencing, MGIEasy Universal DNA Library Prep Set and the User Manual protocol (Cat. No.: 1000006985, 1000006986, 1000017571. Set Version: V1.0. Manual Version: A3), which includes the following steps of DNA fragmentation, selection of DNA fragments by length, end repair and A-tailing, adapter ligation, post-ligation purification, PCR, post-PCR library purification, denaturation, single-strand circularization and product purification, were used (https://en.mgi-tech.com/products/reagents_info/8/). To perform sequencing from ready-made DNA libraries, protocol DNBSEQ-G400RS High-throughput Rapid Sequencing Set User Manual A2 was used.