title: UPEC isolate from community acquired UTI of sample
design: Library preparation was performed at THSTI-NGS facility using Illumina DNA prep/flex kit. Briefly, an input DNA amount ranging between 100ng was tagmented (fragmented and adaptor tagged) and uniquely barcoded. Amplification of the tagmented DNA was done using i7 index adapters followed by library clean up using sample purification ampurev beads on a magnetic stand. Then, individual libraries were quantified using Qubit Hs DNA assay kit in order to achieve optimal cluster density. Finally, library quality was checked using Agilent Bioanalyzer 2100 and typical library size (average size 600bp) was calculated for each sample. Individual libraries were normalized to a concentration of 4 Nano molar followed by pooling of individual libraries to a single eppendorf tube. This pooled library was denatured using NaOH and was sequenced using paired end MiSeq V3 reagents in Illumina MiSEQ sequencer machine. Final loading concentration was kept at 11 Pico molar.