title: DRS of ddm1-L2
design: Total RNA was isolated by Trizol (Qiagen) and poly(A) RNA was purified using Dynabeads mRNA Purification Kit (Invitrogen) following the manufacturers instructions. The quality and quantity of mRNA was assessed using the NanoDrop 2000 spectrophotometer and Qubit. Library was prepared using direct RNA sequencing kit (Nanopore, SQK-RNA002), loaded onto an R9.4 Flow Cell (Flow cell type FLO-MIN106) and sequenced on a GridION device for 72 hours.