title: RNA-Seq of homo sapiens: adult female breast cancer
design: Total RNA was isolated using a GeneJET RNA Purification Kit (Thermol) according to the manufacturers protocol and 1 g of RNA was converted to cDNA using the Hifair III 1st Strand cDNA Synthesis SuperMix (Yeasen) in the presence of both oligo (dT) and random primers. RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).Single-stranded circle DNA molecules are replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA is generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS).