title: GSM6423425: hMeDIP-seq in WT; Mus musculus; MeDIP-Seq
library_construction_protocol: cells were crosslinked with 1% formaldehyde for 10 min and quenched with glycine for 5 min at the room temperature.5 μg of genomic DNA was sheared into 200-500 bp using Bioruptor (Diagenode) for 13 cycles with 30s on, 60s off. Sonicated DNA was immunoprecipitated with 5 μg of 5hmc antibody for 2 hrs at 4  °C, then Protein A/G beads was added into the DNA-antibody mixture and samples were incubated for another 2 hrs at 4  °C. DNA was purified from the elutant using FastPure Gel DNA Extraction Mini Kit (Vazyme). The sequencing library was generated from purified ssDNA (10 ng) with Scale ssDNA-seq Lib Prep Kit (Abclonal) and amplified by PCR. Libraries with different indexes were pooled and sequenced using Illumina HiSeq sequencing systems. hMeDIP-seq