title: GSM7965523: Tg_M_H2O_Pris_D37_CD4_RNA; Mus musculus; RNA-Seq
library_construction_protocol: At 52 weeks post-injection, spleens were harvested and mechanically dissociated. CD4+ cells were isolated from freshly dissociated mouse spleen using the EasySep Mouse CD4+ T cell Isolation Kit (STEMCELL Technologies, 19852). Freshly isolated CD4+ cells were prepared using the stranded mRNA Library Prep (Illumina, 20020594) to generate polyA-selected RNA-sequencing libraries. Cleanup performed on magnets with AMPure XP beads (Beckman Coulter, A63880).