title: E. coli DH10GFP gene expression monitor strain
design: Genomic DNA was isolated using a PureLink Genomic DNA Mini Kit (Invitrogen). For Illumina sequencing, up to 10 ng of plasmid DNA was used as input for sequencing library preparation using the 2S Turbo DNA Library kit (Swift Biosciences) with 20% reaction volumes. Illumina libraries were prepared using 10 g of DNA as input into a 2S Turbo DNA Library kit (Swift Biosciences) using 50% reaction volumes and a final PCR step with custom adapters that added dual 6-bp sample barcodes.