title: GSM8370069: GC145382,HuNoV-infected, replicate 1, scRNAseq; Danio rerio; RNA-Seq
library_construction_protocol: Intestinal tissues were manually dissected and subjected to dissociation using 1mg/mL collagenase, 40 µg/mL proteinase K, 0.25% trypsin for 40 min at 37°C. Cells were resuspended in 0.04% BSA and live cells were selected via Optiprep density gradient according to the manufacterer's protocol. The single-cell suspensions were immediately subjected to the 10X Genomics Chromium Controller with Chromium Single Cell 3′ Library & Gel Bead Kit v3.1. Libraries were constructed according to 10X Genomics Chromium Single Cell 3'Library & Gel Bead Kit v3 protocol.