title: GSM7781824: Tomato root 2 dpi macA∆ biological replicate 2; Fusarium oxysporum f. sp. lycopersici 4287; RNA-Seq
library_construction_protocol: The frozen material was lyophilized using a freeze-drying system and then pulverized using a FastPrep-24™ 5G homogenizer (MP Biomedicals™) and 1-5 mm diameter glass beads. Total RNA was extracted using RNeasy® PLant Mini Kit (Quiagen) and treated with DNAse I using the Turbo DNA-FreeTM Kit (InvitrogenTM) according to the manufacturer's instructions. RNA integrity and quantity were analyzed by electrophoresis on a 1.5% agarose gel and with the Nanodrop® ND1000 spectrophotometer. RNA libraries were prepared for sequencing using standard Illumina protocols