title: GSM7158100: MCF10A,H3K27ac,rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq was performed as previously described (O'Geen et al., 2010). We performed independent replicates for MCF10A, MCF10AT1, MCF10DCIS and MCF10CA1a using H3K4me3 (ab8580), H3K4me1 (ab8895), H3K27ac (ab4729), H3K9me3 (ab8898), and H3K27me3 (ab6002). ChIP-seq libraries were build using NEBNext Ultra II (E7645L) following the manufacturer's instructions.