title: GSM8070199: Treg.meninges.24h.DT#4; Mus musculus; RNA-Seq
library_construction_protocol: For freshly-isolated samples, 500-1000 cells were double-sorted into 5ul TCL buffer (Qiagen) containing 1% 2-mercaptoethanol (Sigma-Aldrich). For whole-tissue RNA-seq, the tissues were harvested and lysed in QIAzol followed by RNA isolation according to the manufacturer's protocol and 2 ng RNA were resuspended in 5 microliters of TCL buffer containing 1% 2-mercaptoethanol. Library construction was performed according to the standard Immunological Genome Project protocols (https://www.immgen.org/img/Protocols/ImmGenULI_RNAseq_methods.pdf).