title: GSM7073829: CKD RASi/SGLT2i/MRA 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using an RNA extraction kit (Life Technologies, Darmstadt, Germany) according to the manufacturer´s instructions, and RNA quality was assessed using agarose gels. RNA-seq of total RNA samples was conducted on the Illumina DNBSEQ-G400 platform, producing 100bp paired-end reads. Strand-specific transcriptome library construction is completed by enriching mRNA from total RNA, sequenced by DNBSEQ high-throughput platform, and followed by bioinformatics analysis.