title: SW480:starve:WES2
design: DNA was extracted using the DNeasy Blood and Tissue Kit (QIAGEN) following manufacturers instructions. Prior to sequencing, DNA was subjected to quality control with Picogreen and Fragment Analyzer to determine DNA integrity. Whole exome sequencing (WES) libraries were prepared using the Agilent SureSelect XT library preparation kit in accordance with the manufacturers instructions.  Briefly, DNA was sheared using a Covaris LE220. DNA fragments were end-repaired, adenylated, ligated to SureSelect oligo adapters, and amplified by PCR.  Exome capture was performed using the Agilent SureSelect XT Human All Exome v6 (60Mb) capture probe set and captured exome libraries were ligated to Agilent Sequencing adapters during target selection and enriched by PCR.