title: GSM7074780: scRNA_multiplexedsample3; Homo sapiens; RNA-Seq
library_construction_protocol: HS and healthy skin tissues were cut into small pieces and were digested with 2.5U/ml Dispase-II (Millipores Sigma, Cat#4942078001) in Hanks' Balanced Salt Solution (HBSS, Corning, Cat#21-022-CM) overnight at 4°C. After digestion, the epidermis was peeled off and minced. The minced epidermis was incubated with 0.05% trypsin-EDTA at 37°C for 40 mins for further digestion. The suspension was then filtered through a 40um cell strainer and spun down at 300g at 4°C for 5 mins. Collected single cells were for the application of SingleCell RNA sequence. Single epidermal cells were loaded into 10xcontroller and individual cells were be captured into oil droplets to form Gel Bead-In-EMulsions (GEMs). The single cell cDNA libraries were constructed according to manufacturer's protocol provided by the Chromium NextGEM single cell 3' reagent kit (10XGenomics, version 3.1).