title: RNA-Seq of leaf in Lv28 under drought treatment
design: Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. These fragments were purified and used as templates for PCR amplification to construct the sequencing cDNA libraries.