title: GSM6423672: ESO26, H3K36me2; Homo sapiens; ChIP-Seq
library_construction_protocol: Ten million esophageal cancer cells were harvested and transferred into 15 ml tubes, followed by fixing with 4 ml of 1% paraformaldehyde for 10 min under room temperature. After quenching and cell lysis, chromatin was sonicated using a Covaris sonicator. Debris was removed by centrifuge and supernatants were diluted five times with the buffer containing 0.01% SDS, 1% Triton X-100, 1.2 mM EDTA pH 8.0, 150 nM NaCl. 1 ug of the H3K36me2 antibody (Cell Signaling Technology, USA, Cat# 2901S) was added and incubated by rotation at 4℃ overnight. Protein G Dynabeads (Life Technologies, USA) were added the next morning and incubated by rotation for an additional 4 hours. Dynabeads were next washed with 1X wash buffer followed by cold TE buffer. DNAs were reverse crosslinked, purified, followed by library preparation and deep sequencing using the Illumina HiSeq platform.