title: GSM6862042: CD_Control; Equus caballus; RNA-Seq
library_construction_protocol: RNA was extracted using Tri-reagent (Sigma), purified using an RNeasy mini Kit (Qiagen, Manchester, UK) and contaminating genomic DNA removed using Ambion DNA-free (Life Technologies, Paisley, UK). RNA concentration was measured using a Nanodrop (ThermoFisher, Loughborough, UK) and a Qubit (ThermoFisher). RNA integrity was confirmed on a Tapestation (Agilent, Milton Keynes, UK). mRNA libraries were prepared using a TruSeq stranded mRNA kit (Illumina, Cambridge, UK).