title: Chinese Yellow Rust 32 with  infected Triticum aestivum cultivar Avocet/Yr7 leaves at 7 days post inoculation
design: After total RNA was extracted by TRIzol reagent kit (Invitrogen, Carlsbad, CA, USA), the RNA molecules in a size range of 1830nt were enriched by polyacrylamide gel electrophoresis (PAGE).  Then the 3 adapters were added and the 36-48nt RNAs were enriched. The 5 adapters were then ligated to the RNAs as well.  The ligation products were reverse transcribed by PCR amplification and the 140-160bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina platform by Gene Denovo Biotechnology Co. (Guangzhou, China).