title: scRNA-Seq of danio rerio: 2dpf head
design: Four brains or three heads per condition from 4dpf or 2dpf larvae respectively were transferred into 1.5ml tubes containing 250 l of papain solution (100l papain, 100l DNase I and 200l L-cysteine per 5 mL of DMEM/F12). The tissue was digested 20min at 37C with pipetting every 10min. Enzymatic digestion was stopped with washing solution (65l glucose 45 %, 50L HEPES 1M and 0.5ml FBS qsp 10ml DPBS 1X) and centrifuged at 800g for 5min at 4C. Supernatant was removed and pellet was resuspended in 50l of washing solution. The cell suspension was filtered with a cell strainer to remove aggregates. Libraries were prepared with a 3' Chromium kit and sent for 10X Genomics sequencing at a depth of 250 million reads (100bp ea) from Illumina NovaSeq6000.