title: GSM8370181: HCE035EDR00, HC distal, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Esophageal mucosal biopsies were homogenized prior to RNA extraction in RLT lysis buffer (Qiagen). Total RNA was extracted using the RNeasy kit (Qiagen, Germantown, MD) according to the manufacturer's instructions Directional mRNA libraries were prepared using NEBNext Kits per the manufacturer's instructions