title: V157/5
design: As Illumina sequencing chemistry is geared towards sequencing short reads, DNA libraries were generated from the pooled amplicons using Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer specifications. The size distribution of the libraries was evaluated using a 2100 Bioanalyzer (Agilent, Santa Clara, USA) and the samples were pair-end sequenced (2 x 300bp) on a MiSeq v3600 cycle (Illumina, San Diego, USA).