title: RRBS of rhesus macaque: whole blood
design: DNA extracted from whole blood sample with Qiagen Blood and Tissue DNA kit, extracted DNA digested using the Msp1 restriction enzyme, ligated NEBNext methylated adapters, bisulfite converted the DNA with Zymo EZDNA Methylation- LightningTM Kit, PCR amplified fragments with NEBNext Multiplex Oligos. RRBS libraries were sequenced on Illumina NovaSeq S4 flowcell (paired end 2x100 bp reads)