title: RNA-seq investigating the influence of astrocytes on developing cortical neurons
design: RNA-seq investigating the influence of astrocytes on developing cortical neurons
library_construction_protocol: Primary cell cultures of cortical neurons and cortical astrocytes were generated from tissue collected from embryonic day 17.5 CD1 mice and embryonic day 20.5 Sprague-Dawley rats. RNA extraction was carried out using the High Pure RNA Tissue kit (Roche) as per manufacturer's instructions. Barcoded RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's instructions.