title: 18S Eukaryotes of mock HF3
design: Enzyme: Thermo Scientific Phusion High-Fidelity PCR Master MixPrimer concentration: 0.4 MCycling and Tm:For 18Sv4 = 10 cycles at 53C + 15 cycles at 48CTAReuk454FWD1    CCAGCASCYGCGGTAATTCC    380 bpTAReukREV3    ACTTTCGTTCTTGATYRA    380 bp