title: GSM7074930: F1/Proestrus-ipsi-4; Mus musculus; RNA-Seq
library_construction_protocol: Dorsal horns of the spinal cord were rapidly dissected and immediately frozen on dry ice. Samples were stored at -80°C untill RNA extraction. Total RNA was extracted using Quiagen Rneasy extraction kit, according to manufacturer's recommendations. P2736 : Full length cDNA were generated from 4ng of total RNA  using SMART-SeqX v4 UltraX Low Input RNA Kit for Sequencing (Takara Bio Europe, Saint Germain en Laye, France) according to manufacturer's instructions with 10 cycles of PCR for cDNA amplification by Seq-Amp polymerase. Six hundreds pg of pre-amplified cDNA were then used as input for Tn5 transposon tagmentation by the Nextera XT DNA Library Preparation Kit (96 samples) (Illumina, San Diego, USA) followed by 12 cycles of library amplification. Following purification with SPRIselect beads (Beckman-Coulter, Villepinte, France), the size and concentration of libraries were assessed by capillary electrophoreris.