title: GSM8162967: Plate 6A, Untreated, DMSO, Drugs; Homo sapiens; OTHER
library_construction_protocol: Cell lysis buffer was be added to each well for RNA isolation at ALI day 21. Lysed cell suspension was first transferred to an oligo-coated plate for mRNA capturing then to a new plate for reverse transcription where barcoded oligos were used. Barcoded cDNA was  combined into one single tube and be prepared for bulk RNA-seq. Triplicate wells were used for each drug to control variation. A library of 441 unique FDA-approved compounds (selleckchem.com)  in 10 Mm DMSO were used.