title: Illumina HiSeq 2500 sequencing; RNA-seq of mouse embryonic fibroblasts(MEFs) with knock-out of SERF2 compared to control MEFs
design: RNA-seq of mouse embryonic fibroblasts(MEFs) with knock-out of SERF2 compared to control MEFs
library_construction_protocol: MEFs were collected by trypsinisation with 0.05 % Trypsin-EDTA (1X), Phenol Red (Invitrogen; 25300-054).  MEFs were cultured in T75 culture flasks (Greiner Bio-One, 658175), high–glucose Dulbecco's modified Eagle's medium (Gibco), supplemented with 10% fetal bovine serum (Sigma 12133C), 1 % penicillin/streptomycin (Gibco), non-essential amino acids (Gibco) and β-mercaptoethanol at 37°C, 5 % CO2 and 3 % O2. E13.5 embryos. Regular mycoplasma tests were performed. Total RNA was isolated from MEFs using the Qiagen RNeasy isolation kit. Integrity of the RNA based on RIN scores as determined by a Bioanalyzer (Agilent).  ). RNA-sequencing libraries were prepared using TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat (RS-122-2201; Illumina, CA, USA) according to manufacturers' protocol.