title: GSM7829913: D7_repeat1; Mus musculus; OTHER
library_construction_protocol: Spleen samples were embedded in OCT compound and flash-frozen immediately after dissection. Fresh frozen tissue was cut into 5μm-thick sections and placed on a 3mm diameter circular array of 10μm diameter mRNA capture beads. Beads were coated in mRNA capture probes consisted of location-specific barcode, a probe-specific UMI, and an mRNA-capturing polyT sequence. Tissue was permeabilised, and mRNAs were captured and ligated to probes. Sequencing libraries were prepared using the Slide-seqV2 protocol (Stickels et al. 2020 - PMID: 33288904).