title: Genomic raw reads from Altica lythri (long reads)
design: For DNA isolation one  A.lythri HT2 male beetle was starved for two days and anesthetize on ice. Then immediatly, the whole beetles was cut in small pieces with scissors and used in a single DNA isolation procedure. DNA was isolated using the Qiagen DNeasy blood and tissue kit, according to the protocol for animal tissue (Qiagen, Hilden, Germany). The only change was the replacing of the spin columns  with magnet-based isolation steps, using 0.4 volumes of AMPure magnetic beads (Beckman Coulter, Krefeld, Germany). In order to obtain a higher concentration of DNA 48 l molecular grade water was used for elution. The DNA concentration was checked with a Qubit analyzer (Thermo Fisher). In addition, size estimation and fragment distribution of DNA was evaluated with Fragment Analyzer (Agilent Technologies, Waldbronn, Germany). DNA isolation procedure as described above yielded 1.5 - 1.8 g total DNA. We used the whole extracted DNA for library production with a ligation sequencing approach,resulted in 15 l per sample (Oxford Nanopore SQK LSK-109). The concentration of the library samples were checked with a Qubit analyzer and ranges from 350 - 500ng. Finally, the prepared libraries were sequenced in two sequencing runs using single flow cells (v.10.3) on the MinIon platform, each of which yielded between 15 - 25 Gbp of long-read information.