title: RNA-seq clinical validation
design: RNA from fibroblast and lymphoblastoid/lymphocyte samples was processed using the Illumina Stranded mRNA prep kit. RNA from whole-blood samples was processed using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit to remove human globin RNA and rRNA. Sequencing was conducted on the Illumina NovaSeqX platform which produces pared-end short read data at 150bp. Each validation sample was sequenced to a target depth of 150 million.