title: GSM7235279: Biol rep 4 CSPG-negative cells; Gallus gallus; RNA-Seq
library_construction_protocol: mRNA was extracted from the FACS -isolated CSPG-positive and CSPG-negative cells using Single Cell RNA purification kit (Norgen Biotek Corp). Each RNA sample had a RIN >6.9. Libraries were prepared using the KAPA Stranded mRNA-Seq Kit (KR0960, Roche, USA) and Illumina platforms sample preparation protocol (v3.15) according to manufacturer's protocoal.  RNA sequencing was conducted by Illumina NextSeq 2000 machine using NextSeq 2000 P2, 100 cycles kit (Illumina, USA). The output was ~25 million single-end- 120bp reads per sample.