title: RNA-seq of mus musculus: adult conditional Reelin knockout hippocampus rep3
design: RNA was extracted from freshly dissected mouse hippocampus using TRIzol (Invitrogen) according to the manufacturers instructions. The quality and rough concentration of the RNA was assessed before submitting 8  RNA samples for library preparation and RNA-sequencing (n=4 hippocampal RNA samples per control and Reelin cKO condition). Libraries were prepared with the Illumina TruSeq Stranded Total RNA Library Preparation kit and run on NextSeq550 (75-bp single-end reads) by the UTSW Genomics & Microarray Core Facility.