title: GSM7235283: VSMCs - GEX; Mus musculus; RNA-Seq
library_construction_protocol: Four groups of vascular smooth muscle cells  cultured on 6-well plate different conditions (Young, Old, Young + Angiotensin II, Old + siPiezo1) were harvested by 0.25% Trypsin-EDTA (Gibco) and cell viability was confirmed using an automated cell counter (Biorad, TC20). Cell hashing with anti-mouse oligo-tagged antibodies (TotalSeq A, Biolegend) were performed according to the manufacturer's protocol before mixing them in single-cell suspension. The libraries were prepared using Chromium Single Cell 3′ Library & Gel Bead Kit v3.1, PN-1000268, the Chromium Next GEM Chip G Single Cell Kit PN-1000120 and theDual Index Kit TT, Set A PN-1000215,(10x Genomics). Amplified cDNA was evaluated on a Agilent BioAnalyzer 2100 using a High Sensitivity DNA Kit (Agilent Technologies) and final libraries on a Agilent TapeStation 4200 using High Sensitivity D1000 ScreenTape (Agilent Technologies).