title: Genomic raw reads from Altica lythri (Illumnia paired-end reads 350bp)
design: For DNA isolation one  A.lythri HT2 male beetle were starved for two days. DNA was isolated using the Qiagen DNeasy blood and tissue kit, according to the protocol for animal tissue (Qiagen, Hilden, Germany). The whole beetle were frozen in liquid nitrogen, ground using a teflon pestle, the resulting powder mixed with the lysis buffer and followed the manual instructions. The only change was the replacing of the spin columns  with magnet-based isolation steps, using 0.4 volumes of AMPure magnetic beads (Beckman Coulter, Krefeld, Germany). The DNA amount was checked with a Qubit analyzer (Thermo Fisher). DNA isolation procedure as described above yielded 0.63 g total DNA. The DNA was sending of for library preparation and Illumina sequencing to Novogene. Mate-pair libraries (2x 150 bp) were created for 350bp fragment size. The sequencing run yielded in a raw data size of 24.6 GB.