title: 16S rRNA amplicons: CattleM-ATS microbiome 3
design: Microbial biofilm sample was frozen in liquid N2, lyophilized and homogenized using Precellys homogenizer (three times at 6,500 rpm for 30 s, Peqlab, Erlangen, Germany) to ensure uniform distribution of samples. Genomic DNA was extracted as previously described by Zhou et al. [33]. The 16S rRNA gene libraries were constructed using universal primer pair 341F and 785R [34] ( for amplification of the V3V4 hypervariable region of the 16S rRNA gene) and applying the 16S Metagenomic Sequencing Library Preparation protocol (Illumina Inc., USA). The constructed libraries were equimolar pooled and subsequently sequenced on the Illumina MiSeq platform applying the protocol for 2x300 bp paired-end reads.