title: RNA-Seq of Angelica gigas: TGT T1 leaves
design: MaterialWe cultivated  Angelica gigas (AG) in a temperature gradient tunnel (3 m width, 3 m height, 27 m length) which created a temperature gradient from the outside to the farthest end. From April to October, AG was exposed to higher temperatures for an extended period at the National Institute of Horticultural and Herbal Science, Pyeongchang, Republic of Korea. mRNA samples were collected from AG leaves at increments of 1C, 24C, and 46C higher than the outside temperature, labeled as AG1, AG2, and AG3, respectively.MethodsmRNA was extracted using the Qiagen RNA Extraction Kit, and RNA sequencing was performed by Macrogen.