title: ITS amplicons: mWW-ATS microbiome ITS 1
design: Microbial biofilm sample was frozen in liquid N2, lyophilized and homogenized using Precellys homogenizer (three times at 6,500 rpm for 30 s, Peqlab, Erlangen, Germany) to ensure uniform distribution of samples. Genomic DNA was extracted as previously described by Zhou et al. [33]. The ITS (internal transcribed spacer) amplicon libraries were constructed using the universal primer pair ITS1 and ITS2 [35] (for amplification of the ITS region between the 18S and 5.8S rRNA gene) and applying the 16S Metagenomic Sequencing Library Preparation protocol (Illumina Inc., USA). The constructed libraries were equimolar pooled and subsequently sequenced on the Illumina MiSeq platform applying the protocol for 2x300 bp paired-end reads.