title: Porcine Epidemic diarrhea virus sample
design: Total RNA was extracted using Viral Nucleic Acid Extraction Kit (Geneaid, Taiwan, China). Primers used in for complete genome sequencing were designed based on conserved regions of the full-length sequences of PEDV after multiple-sequence alignment. Meanwhile, the 5 and 3 terminal ends of the virus genome were amplified using the SMART 5 RACE & 3 RACE (Clontech, Japan) and a Gene-Specific Primer (8), respectively, following the manufacturers instructions. The PCR amplicons were gel purified and cloned into the pEasy-blunt vector (TransGen Biotech, Beijing, China). Three or four clones from each PCR product were sequenced by Sangon Biotech (Shanghai, China). Finally, the date data was assembled and annotated by Lasergene version 7.0 with default parameters.