title: RNAseq for mutant nac130
design: Total RNA was extracted from the maize tissues using TRIzol reagent (MRC, Molecular Research Center, Inc.) according to the manufacturers instructions. A Bioanalyzer 2100 was used to check the quality of the extracted RNA, and only RNA of high integrity was used for the analysis. TruSeq RNA Sample Prep Kit v2 (Illumina, RS-122-2001) or NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) was used according to the manufacturers recommendations. The samples were pooled and sequenced (150-bp paired-end reads) on the Illumina NovaSeq 6000 platform.