title: GSM7074343: Duodenum organoids from CDK2-dTAG mice, DMSO treated, 24 hours, replicate #1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were harvested by scraping, washed with ice-cold PBS, and RNA was extracted using Qiagen's RNeasy Micro kit (Qiagen catalog # 74034) following manufacturers recommendation 50 ng RNA was used as input for the Lexogen QuantSeq 3' mRNA-Seq Library Preparation kit for Illumina (FWD) (Lexogen, catalog # 015.96) and libraries were prepared according to the manufacturer's protocol