title: GSM8370035: MLO_WT_batch2_manual_90; Homo sapiens; RNA-Seq
library_construction_protocol: Following manufacturer's protocol, 50 units of papain enzyme (Worthington) was activated with 1 mM L-cysteine and 0.5 μM EDTA in Earle's Balanced Salt Solution (EBSS) (Thermo Fisher), and DNase I (Worthington) added. hMLOs were finely chopped with a scalpel and then resuspended in papain enzyme mix. The slurry was incubated at 37°C, 5% CO2 for 45 min on a rotator, and then triturated. Cells were spun down at 300xg for 5 min and washed twice in EBSS containing ovalbumin (Worthington) and BSA. Library preparation for RNA-seq was performed by Novogene AIT Singapore or using Chromium Single Cell 3ʹ GEM kit.