title: GSM6659102: Treg_FE_Rep1; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were removed, gently dissociated on ice, and cells were antibody-stained and sorted by FACS (Aria III). Cells were activated in vitro with plate-bound anti-CD3 (2μg/ml), anti-CD28 (2μg/ml) and IL-2 (10 ng/mL) for 72h. RNA was harvested and isolated using NucleoZOL reagent and columns. QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina was used with 500 ng of total RNA for the construction of sequencing libraries. UMI were added to the protocol. Libraries were prepared for sequencing using standard protocols 3'-RNA-Seq