title: GSM8438569: E-17.5 WT cortical ECs-1 (biol rep 1); Mus musculus; RNA-Seq
library_construction_protocol: E17.5 WT and HDAC2 ECKO - The sorted cells were frozen on dry ice as soon as the sorting is done. Libraries were prepared with SMART-Seq V4 PLUS Kit (R400752, Takara Bio, Japan) following the manufacturer's instructions RNA with an integrity number of greater than 7 was used for library preparation. Libraries were prepared with KAPA mRNA HyperPrep (KK8581, Roche) and KAPA UDI Adapter Kit 15μM (KK8727, Roche) following the manufacturer's instructions. RNA-seq. For E-17.5 WT and HDAC2 ECKO - The libraries were pooled evenly and went for the paired-end 150-cycle sequencing on an Illumina NovaSeq X plus System