title: Identification of novel and already known altered micoRNAs lead to a deeper understanding of the development of type 1 diabetes mellitus
design: Identification of novel and already known altered micoRNAs lead to a deeper understanding of the development of type 1 diabetes mellitus
library_construction_protocol: Overnight fating peripheral blood samples from all subjects were collected in EDTA-containing Vacutainer tubes (BD Biosciences). Blood samples were immediately centrifuged at 2000 x rpm for 15 minutes at 4˚C. The top layer containing the plasma was divided into aliquots and stored at -80˚C until further analysis. Total RNA was isolated from 200 µl of plasma using the miRNeasy Serum/Plasma kit (Qiagen, Germany) in accordance with the manufacturer's recommendations. 2µg of total RNA per sample were used as input material for small-RNA cDNA libraries generation using NEBNext Multiplex Small RNA Library Prep Set for Illumina® (New England Biolabs, United States).