title: GSM8368411: OCDC, scRNA; Mus musculus; RNA-Seq
library_construction_protocol: scRNA-seq was performed by the 10x Genomics single-cell 5′ V(D)J library platform. scRNA-seq was performed by the 10x Genomics single-cell 5′ V(D)J library platform. The cell suspension was loaded into Chromium microfluidic chips with 30 (v2 or v3, depending on project) chemistry and barcoded with a 10× Chromium Controller (10X Genomics). Single-cell gene expression and TCR libraries were generated according to the manufacturer's instructions. RNA from the barcoded cells was subsequently reverse-transcribed, and sequencing libraries were constructed with reagents from a Chromium Single Cell 30 v2 (v2 or v3, depending on the project) reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (HiSeq 2000 or NovaSeq, on the project) according to the manufacturer's instructions (Illumina).