title: GSM7235231: ARPE19 cells, RNA-Seq, normal control; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from ARPE19 and WERI-RB1 cells using the TRIzol reagent (Invitrogen). RNA purity and concentration were confirmed on a 2100 Bioanalyzer (Agilent Technologies) and a Qubit 2.0 fluorometer with the Qubit RNA Assay Kit (Life Technologies). 2 μg total RNA per sample was used as input material for the RNA sample preparation. Library preparation was completed using the VAHTS Total RNA-seq Library Prep Kit for Illumina (Vazyme) following the manufacturer's recommendations. The libraries were sequenced by the Illumina PE150 paired-end sequencing platform.