title: GSM7073963: H_siK1_input; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from scrambled or VIRMA-silenced SUNE-1 and HONE-1 cells and analyzed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, Santa Clara, CA, USA) with a RNA integrity number (RIN) number > 7.0. After RNA purification and fragmentation, RNA samples were incubated with anti-m6A primary antibody (Synaptic Systems) for immunoprecipitation using a Magna MeRIPTM m6A kit (Millipore) according to the manufacturer's instructions. Enriched m6A-modified RNA was then eluted and subjected to next-generation sequencing using the Illumina NovaseqTM 6000 platform (Illumina, San Diego, CA, USA) at LC-BIO Biotech Ltd (Hangzhou, China).