title: Rna-Seq of human leukemia cells from T-ALL PDX
design: Total RNA was isolated by TRIzol (Invitrogen) following manufacturer's standard protocol (TRIzol Reagent; Invitrogen) from FACS-sorted EPHB6 + and EPHB6- cell subsets from 3 independent clones of PDX samples. Library preparation and sequencing of ribodepleted total RNA was performed in service at the BC Cancer Genome Sciences Centre (Vancouver), reaching about 25 million reads for sample. The quality of the RNA was assessed by Quibit RNA assay and Agilent Bioanalyzer.