title: Targeted genetic therapy for congenital melanocytic naevi
design: Targeted genetic therapy for congenital melanocytic naevi
library_construction_protocol: Primary naevus cells were isolated from patients with CMN by growing cells in vitro using melanocyte media RNA was extracted using the RNAeasy plus mini kit (Qiagen, Germany), using RLT buffer to lyse cells, 70-90% ethanol to precipitate the nucleic acids and nuclease free water to resuspend the RNA. cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche)