title: tumor: Sample SU2CLC-MGH-1573-T1-RNA
design: RNA was extracted from FFPE tumor specimens. Extraction was performed using the Qiagen AllPrep DNA/RNA Mini Kit (cat# 80204). A single aliquot of 10-500 ng input RNA in 100 μl TE buffer was used for library generation. Library preparation was performed using the Kapa HyperPrep kit, and quantification was performed using PicoGreen. Adapter ligation was performed using the RNA Prep with Enrichment kit from Illumina per manufacturer's instructions. Sequencing of pooled libraries was performed using a HiSeq4000 with 76 bp paired end reads.