title: GSM6423670: ESO26_WGBS; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Following DNA extraction and quality control (QC), 3 ug of DNA which was spiked with 26 ng lambda DNA were fragmented by sonication. Cytosine-methylated molecular barcodes were ligated to the fragmented DNA. Bisulfite conversion was performed using EZ DNA Methylation-GoldTM Kit (Zymo Research) according to the manufacturer's instructions. DNA fragments were then PCR amplified by HiFi HotStart Uracil and ReadyMix (Kapa Biosystems). The clustering of the index-coded DNA samples was performed by Illumina cBot Cluster Generation System, followed by sequencing using Illumina Hiseq platform.