title: 16S metabarcoding sequences of Littorina fabalis microbiome: intestinal tissue
design: The DNA extraction from dissected guts and tantacles of L. obtusata and L. fabalis as well as from the environmental scrapings was performed with the DNeasy PowerSoil kit (QIAGEN). The 16S rDNA libraries preparation for the Illumina MiSeq sequencing included the following steps: [1] the locus-specific PCR, using 515F and 806R primers from Caporaso et al. 2011; [2] the amplicon clean-up with the magnetic beads (Evrogen); [3] the indexing PCR and the 2nd clean-up with the magnetic beads; [4] the final pooling.