title: NextSeq 500 sequencing; Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis
design: Identification of Campylobacter jejuni and Campylobacter coli genes contributing to aerobic stress response using TraDIS analysis
library_construction_protocol: Transposon libraries were grown for 16 hours in MH broth with or without H2O2 under microaerophilic conditions.  DNA was extracted using Qiagen g20 tips and genomic buffer system. Nucleic acid was tagmented using Nextera XT tagmentation kit. PCRs were performed using the illumina P7 primer and a customised P5 primer that hybridized to the end of the transposon. DNA was quantified using Quant-IT ds DNA HS assay kit.