title: GSM6922986: Sample4NSD; Mus musculus; RNA-Seq
library_construction_protocol: Mouse brain section per mouse was cut at 10 µm thickness and mounted onto each Visium slide capture area. After H&E staining, each bright-field image was taken as described in the Spatial Transcriptomics procedure. Tissue permeabilization was performed for 18 minutes, as established in the tissue optimization assay. The Visium Spatial Gene Expression Slide & Reagent kit (10X Genomics) was used to generate sequencing libraries for Visium samples. Libraries were constructed according to the 10x Visium library construction protocol and sequenced by Illumina NovaSeq6000.