title: Rhizosphere.Soil.DNA_Vaccinium_spp_Plot1
design: 150 mg of sediment samples were used for total genomic DNA extraction using the QIAGEN PowerSoil kit. The V3-V4 region of the 16S rDNA, and the fungal ITS rDNA were then amplified in a PCR reaction using the 341F/805R staggered primer pair for the 16S and ITS3_KYO2F/ITS4R for the ITS, all of which containing the Illumina overhanging adapters. A limited cycle PCR followed in order to attach sample-specific indexes. Amplicons were pooled at equimolar concentration. The amplicons were sequenced using 2x300bp paired-end sequencing using the Illumina Miseq platform. The resulting sequences were de-replicated.