title: RNAseq of untreated, CSF1-treated, CSF1+QVD-treated, and GM-CSF-treated monocytes
design: RNAseq of untreated, CSF1-treated, CSF1+QVD-treated, and GM-CSF-treated monocytes
library_construction_protocol: Human peripheral-blood monocytes were obtained from healthy donors. CD14+ monocytes were purified using CD14 microbeads with a light-scattering (LS) column according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). CD14+ were treated with CSF1 at 100 ng/mL during 3 days CD14+ were treated with CSF1 at 100 ng/mL and QVD at 50 Î¼M during 3 days CD14+ were treated with GM-CSF at 100 ng/mL during 3 days RNA were extracted with the RNeasy mini kit (QIAGEN) SureSelect Automated strand specific RNA library prep.  mRNA library prep for Illumina paired-end multiplexed sequencing.