title: GSM8438326: Human NK stimulated with IPH6501 (4h), CMO; Homo sapiens; RNA-Seq
library_construction_protocol: After incubation (4 hours or 24 hours), plates were washed in complete RPMI medium and  centrifuged 400g for 5 minutes at +5 ± 3°C. Cells were washed once with PBS + 1% BSA and centrifuged at 400g for 5 minutes at +5 ± 3°C. Samples from the same conditions of all 4 donors were individually stained with barcoded lipids from 3' CellPlex kit Set A (10X Genomics, #1000261). After pooling, samples from the same conditions of all 4 Healthy Donors  were individually stained with barcoded lipids from 3' CellPlex kit Set A (10X Genomics, #1000261): cells were centrifuged at 400g for 5 minutes at +5 ± 3°C, and resuspended in either 100 µL of individual barcode from 3' CellPlex kit Set A reagent (10X Genomics, #1000261). Following recommendations of 10X Genomics, cells were resuspended in 50 µl PBS 1% BSA. Libraries were prepared with Chromium Next GEM Single Cell 3' v3.1 kit (10X Genomics). For each condition, 100 ng of Gene Expression Library were mixed with 25 ng of Cell Multiplexing Oligos Library.