title: Rat3_RDG_DRS
library_construction_protocol: Isolated brain tissue (dentate gyrus) was homogenized in 1 mL of Trizol. RNA was then extracted from supernatant using standard manufacturer's protocol. RNA was quality assessed using Nanodrop 2000 Spectrophotometer, then subjected to further purification by precipitation with 3 volumes of KAPA Pure Beads according to manufacturer's instructions. 4-5-5 μg of total RNA was mixed with 250 ng of oligo(dT)-enriched RNA from Saccharomyces cerevisiae and 5 ng of in vitro transcribed poly(A) standards. It was then used to prepare libraries with a Direct RNA Sequencing Kit (SQK-RNA002) from Oxford Nanopore Technologies (ONT), following the manufacturer's instructions. Sequencing was performed using R9.4.1 RevD flow cells on a MinION device, controlled by MinKNOW software (ONT) as described by Bilska et al. (2020).