title: Whole-genome CRISPR knockout screen in A549 cell line to identify important host determinants forIAV infection
design: For PCR amplification products that do not contain any sequencing connector sequence, after passing the quality inspection, 100~500ng DNA fragments were extracted and subjected to End Repair, 5 'Phosphorylation and dA-Tailing, Adapter Ligation, Clean Up, PCR Enrichement and Clean Illumina platform high-throughput sequencing library was constructed by Up and other steps.