title: RNA-seq of Homo sapiens: Periodontal Ligament Stem Cells (48 hours of differentiation) replicate 1
design: RNA was isolated using ExtractRNA (Eurogene) by standard phenol/chloroform extraction procedure in accordance with the manufacturer's instructions. RNA quality was assessed by agarose electrophoresis and Nanodrop spectrometry. For RNA-seq library preparation, 500 ng of each sample was used with the CORALL Total RNA seq Library Prep Kit (Lexogen), which included poly-A RNA selection according to the manufacturers recommendations. The quality of the obtained libraries was verified by capillary electrophoresis using an Agilent Bioanalyzer 2100 (Agilent Technologies). Finally, the libraries were sequenced on the Illumina NextSeq550 platform using single-ended reagents. All samples were run simultaneously.