title: Microbiome of Rhabdastrella sp.
design: Ribosomal V4 region amplified with 515F-Y (Parada et al., 2016) and 806R (Apprill et al., 2015), and the Illumina adapter overhang in both primers for a dual-barcoding approach. Amplification using PCRBIO HiFi Polymerase (PCR Biosystems Ltd) under the following conditions: 95C for 3 min, followed by 28 cycles of 95C for 20 s, 60C for 20 s and 72C for 30 s, and final elongation step at 72C for 5 min. PCR products were purified with Agencourt AMPure XP Beads (Beckman Coulter Inc.), and libraries prepared with the Nextera XT DNA Library Preparation Kit (Illumina Inc.).