title: RNA-seq data sets of human non small cell lung cancer line A549 treated with feiyanning against untreated controls, during co-culture with macrophages.
design: RNA-seq data sets of human non small cell lung cancer line A549 treated with feiyanning against untreated controls, during co-culture with macrophages.
library_construction_protocol: A549 cells and macrophages were incubated 48h together in serum-free medium for producing co-cultured A549 cells. Treated groups were supplemented with Feiyanning as required. Feiyanning formula (FYN), a proprietary prescription developed by Z-YX, a prominent TCM practitioner in Shanghai. Feiyanning formula (FYN) is a traditional Chinese medicine (TCM) prescription used for more than 20 years in the treatment of lung cancer. FYN is composed of Astragalus membranaceus, Polygonatum sibiricum, Atractylodes macrocephala, Cornus officinalis, Paris polyphylla, and Polistes olivaceous, etc. Total RNA samples were extracted using TrizoI. The mRNA was fragmented and reverse transcripted into cDNA after retained using Oligotex mRNA Mini kit according to the manufacturer's protocol. The fragments were purified, end repaired, poly(A) added sequentially, and ligated to Illumina adapters. The final products were amplified after User enzyme digestion.