title: Shotgun-Metagenome of soil
design: DNA samples are shredded to 200-500bp DNA fragments. Then, adaptors are inserted at both ends of the DNA fragments through reduced-cycle amplification. An adaptor has a sequencing binding primer region, an index and an oligo at the end. The adaptors are specific for the 5' and 3' end, and they differ in their sequencing binding site and the small oligo while sharing the same index. The strategy allows the unequivocal assignment of the fragments to the correct sample in the multiple concomitantly sequenced samples, something referred to as multiplexing.