title: Prokaryote 16S rRNA gene amplicons from nitrifying soil microcosms
design: The total concentration of microbial DNA was measured using the Qubit High Sensitivity assay (Thermofisher) and normalized at 1 ng/l prior to PCR amplification of the V4 region of the 16S rRNA gene. The amplification was carried out using V4 primers 515F/806R (Walters et al. 2015) followed by Illumina Miseq sequencing preparation with the MiSeq Reagent Kit v2 (500-cycles)