title: 16S rRNA gene amplicon of bovine: feces
design: Fecal samples were collected from rectum. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted as described by Rius et al. (2012). For microbial amplicon sequencing universal primers targeting 16S rRNA gene V4 region were used (Caporaso et al. 2011).