title: isoCirc RNAseq of Lotus Leaf
design: CircRNA encrichment and nanopore sequencing according to isoCirc Protocol. Total RNA extraction, ribosomal RNA depletion via Ribominus, poly A tailing and poly A pulldown, RNAse R Digest, reverse transcription and isothermal amplification with random hexamer primers. Nanopore sequencing on one lane of Minion flowcell vR9