title: RNA seq of Bacillus subtilis: pH5 stress CSD21 parallel 2
design: The gene expression was analyzed with the Htseq software (V 0.6.1) using the FPKM (Fragments Per Kilobase per Million reads) method. The differential expression analysis was conducted using the DESeq2 (V1.6.3) package from Bioconductor. The cutoff of the differentially expressed gene was set at a log2-fold change > |1| with a q-value (FDR, padj) < 0.05.