title: BGI resequencing of ddm1 #3
design: The genomic DNA for each plant was extracted based on the CTAB method. Then 1 g genomic DNA was randomly fragmented by using ultrasonicator (Covaris). An average size of 200-400 bp DNA fragments were selected by Agencourt AMPure XP-Medium kit. The fragments were then end repaired, 3 adenylated and ligated with adaptors. The purified double stranded products were heat denatured to single strand, and then circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library and qualified by QC. The final qualified libraries were sequenced by a DNBSEQ-T7 sequencer. An ssCir DNA molecule formed a DNA nanoball (DNB) containing more than 300 copies through rolling-cycle replication. The DNBs were loaded into the patterned nanoarray by using high density DNA nanochip technology. Finally, pair-end 150 bp reads were obtained by combinatorial Probe-Anchor Synthesis (cPAS).