title: GSM6499592: NFD_3; Oreochromis niloticus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturer's procedure The sequencing was performed with three bio-replicates per group (RNA from three samples per group was mixed), so that six cDNA libraries was constructed (HFD_1, HFD_2, HFD_3, NFD_1, NFD_2, NFD_3) using the mRNA-Seq sample preparation kit (Illumina, USA) after removal of rRNA by Epicentre Ribo-Zero Gold Kit (Illumina, USA), following standard procedure.