title: RNA-Seq of Homo sapiens: HEK293FT cells
design: RNA-seq experiments were performed by the Max Planck Genome Centre (MPGC) Cologne, Germany (https://mpgc.mpipz.mpg.de/home/). RNA Quality was assessed by Agilent Bioanalyser (Nanochip). Library preparation was done according to NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) including polyA enrichment and addition the of ERCC RNA spike-ins. Libraries were again quality controlled by Agilent Tapestation or LabChip GX or GX Touch (PerkinElmer). Sequencing-by-synthesis was performed on a HiSeq 3000 (Illumina) with single read mode 1 x 150 bp.