title: GSM7830291: anther, Mv 17-09 treated rep2; Triticum aestivum; RNA-Seq
library_construction_protocol: Anther tissue was ground using a mortar and pestle in liquid nitrogen. Total RNA was extracted using Direct-zol™ RNA MiniPrep Kit, following the manufacturer's protocol (Zymo Research, Irvine, CA, USA). The libraries for RNA-seq were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, Ipswitch, MA, USA). Briefly, ribosomal RNA was depleted from 400 ng of total RNA using the QIAseq FastSelect rRNA Plant Kit (Qiagen, Hilden, Germany). The depleted RNA was fragmented, end-prepped, adapter ligated, and the libraries were amplified according to the manufacturer's instructions.