title: Polystyrene nanoplastics treatment Rhizosphere soil bacteria repeat 2
design: The bacterial 16S rRNA gene V3-V4 region and fungal ITS-V1 region was amplified using primers 338F/806R, respectively. Sequence libraries were constructed using the VAHTS Universal Plus DNA Library Prep Kit for Illumina using purified PCR products. With the PE250 strategy, sequencing was performed on the Illumina NovaSeq6000 platform.