title: GSM8370458: kidney sample 103; Homo sapiens; RNA-Seq
library_construction_protocol: The human kidney tissue specimens were promptly submerged into RNALater (Sigma) and kept in -80℃ to preserve the integrity of RNA. Manual microdissection techniques were employed to carefully isolate the glomerular and tubular compartments from the tissue. Subsequently, the dissected tubular tissues underwent homogenization, and RNA was purified using the RNeasy Mini Kit (Qiagen) along with DNase treatment (Qiagen). RNA libraries were prepared for sequencing using standard NEBNext protocols