title: targeted UCE sequncing of homo sapiend: CLL blood
design: The sheared DNA was then used for library preparation using a KAPA library preparation kit following the with beads manufacturer protocol. Briefly, this protocol consists of 3 enzymatic reactions for end repair, A-tailing, and adaptor ligation followed by barcode insertion via polymerase chain reaction (PCR) using KAPA HiFi polymerase (6 cycles). PCR primers were finally removed using a 1.8  volume of AMPure PCR Purification Kit (Agencourt Bioscience Corporation).