title: GSM7964448: Input mixed embryos R1; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: ChIP-seq: Embyros were fixed with 1.8% paraformaldehyde in 1XPBS with 0.5% Triton-X 100 and heptane. Fixed embryos were ground using a pestle in RIPA buffer and chromatin was sonicated using a probe sonicator at 20% output for 9x 20 seconds. ChIP-seq: Libraries were constructed using the NEBNext ChIP-seq kit (New England Biosystems, E6240L). CUT&RUN: Libraries were constructed using the Kapa HyperPrep Kit (Roche) and IDT Adapters and the NEBNext Ultra II DnA Library Prep Kit.