title: Long-read WGS of Candida albicans: SC5314 CRISPR-competent tlo-null lineage 2 a/alpha
design: -80C frozen stock was struck to solid YPD medium and incubated at 30C for 2 days. Cells from the densest part of the streak were then used to start an overnight culture in liquid YPD medium at 30C. The next day, ~10^8 cells were pelleted and sent to the University of Wisconsin-Madison's Biotechnology Center (UWBC). UWBC performed high molecular weight DNA extraction on the samples to prepare Oxford Nanopore barcoded libraries, with DNA fragment lengths being on average ~20 kb. Libraries were then sequenced on a PromethION 24 using a FLO-PRO114M (R10.4.1) flow cell and an SQK-NBD114-24 (V14) kit for 12 hours. Prior to data delivery, basecalling was performed by UWBC using Guppy 6.4.6 (high-accuracy model, 400 bps) and reads were demultiplexed.