title: NextSeq 500 paired end sequencing of SAMD00405558
library_construction_protocol: 100 ?l of peripheral blood was collected from 2 eight-week-old mice through retro-orbital bleeding into tubes coated with K3-EDTA (Greiner). Genome DNAs were purified from the whole blood using QIAamp DNA micro kits (Qiagen) according to the manufacturer?s instructions. Transverse aortic constriction (TAC) was performed on these 2 mice, and 4 weeks later blood collection and purification of genome DNAs as post-TAC samples were performed. Genome DNAs were converted to DNA libraries for next-generation DNA sequencing with an Agilent SureSelect XT HS Reagents kit (Agilent).