title: RNAseq of mus musculus: fetal brain tissue
design: Whole fetal brains were randomly chosen from each litter, dissected, and immediately placed into 400uL RNAlater (ThermoFisher Scientific). RNA was extracted and cDNA synthesized using the PureLink RNA Mini Kit (Invitrogen) and qScript cDNA synthesis kit (Quantabio). Equal amounts of RNA were pooled from 2 brains per litter. An RNA integrity number (RIN) of at least 8.7 was confirmed for each sample using the 4200 Tapestation system (Agilent). RNA was prepared using the TruSeq RNA Library Prep kit and Lexogen QuantSeq 3 forward-sequencing was performed using the Illumina HiSeq 4000 platform.