title: GSM7829921: D7 HTO; Mus musculus; RNA-Seq
library_construction_protocol: Polyclonal CD4+ T cells from spleens were isolated by flow cytometry into a 2% BSA/PBS buffer. Samples from each individual mouse replicate were labelled with a different hashtag reagent, and samples from 5 individual mouse replicates were pooled for isolating CD11a-high/CXCR3+ CD4+ T cells. Polyclonal and TCR transgenic cells were sortedseparately and pooled at a proportion of 9:1. Cells were sorted as single-cells using the Chromium 10x Genomics platform. Approximately 33,000 cells were loaded per channel onto a Chromium controller (10x Genomics) for generation of gel bead-in-emulsions using 10x Genomics Single Cell 5' Reagent Kit v2 (single-cell gene expression and immune repertoire profiling). Sequencing libraries were prepared using Single Cell 5' Reagent Kits v2 (10x Genomics) and then converted using the MGIEasy Universal Library Conversion Kit (BGI).