title: TA17
design: Extraction of microbiome DNA was performed using the DNeasy PowerSoil Pro Kit (Qiagen,Germany) following the manufacturers instructions. Minor modifications were made to optimize theDNA extraction. In brief, 750 l of sample supernatant was centrifuged at 21,000  g for 5 minutes.The pellet was dissolved in Solution CD1, transferred into PowerBead Pro Tubes (Qiagen, Germany)and incubated at 65C for 10 minutes. With the use of a MagNA Lyser Instrument (Roche AppliedSciences, Germany), samples were lysed twice at 6,000  RPM for 30 seconds each. Finally, 70 l ofSolution C6 was added and incubated at room temperature for 5 min before centrifugation. DNAconcentrations were determined fluorometrically using a Qubit Fluorometric Quantitation HSdsDNA Assay kit on a Qubit 4.0 Fluorometer (Thermo Fisher Scientific, USA). The DNA wasfragmented and 5&#39; Adapter:5&#39;-AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATT-3&#39;, and 3&#39; Adapter:5&#39;-GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATGACTATCTCGTATGCCGTCTTCTGCTTG-3&#39;adapters were used. Shotgun sequencing was conducted on an Illumina NovaSeq 6000 instrument(Illumina, USA) with a 150-bp paired-end sequencing run at Novogene Co. Ltd, China. The sequencingyielded a minimum of 20 million reads per sample. To ensure the availability of 20 million reads persample for shotgun sequencing, we achieved this by re-isolating from each sample until obtaining therequired purity OD260/280=1.8-2.0 and concentration  10 ng/L for each metagenomic isolate.