title: NextSeq 500 sequencing; Blood flow diverts extracellular vesicles from endothelial degradative compartments to promote angiogenesis
design: Blood flow diverts extracellular vesicles from endothelial degradative compartments to promote angiogenesis
library_construction_protocol: HUVECs cultured in static or flow conditions and treated with 1.109 EVs or PBS for 24h Total RNA was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany) and RNA integrity was assessed by Bioanalyzer (total RNA Pico Kit, 2100 Instrument, Agilent Technologies, Paolo Alto, USA) SMART-SeqÂ® HT PLUS Kit (Takara, Kusatsu, Japan) was used to build mRNA libraries.