title: GSM8368756: MALE, ES, d14, rep2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq, total cellular RNA was extracted using TRIzol (Thermo Fisher). rRNA was subsequently removed from the extracted RNA using using the NEBNext rRNA Depletion Kit . RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs), which enabled the creation of strand-specific RNA-seq libraries