title: ChIP-seq of wild type P. falciparum NF54 parasites using IGG antibody
design: Infected RBCs were collected at 16 h.p.i., lysed using 0.1% saponin (Sigma) and cross-linked in 1% methanol-free formaldehyde (Polysciences Inc). Chromaitin was sheared using the Covaris ME220 ultra sonicator and immunoprecipitated using non-immune rabbit IgG as negative control (12-370, Upstate). ChIP-seq libraries were prepared using the KAPA LTP library preparation kit (KAPA Biosystems), then amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) and purified with 1X KAPA Pure beads (KAPA Biosystems).