title: GSM7782983: KMT2Ar005 (Hi-C); Homo sapiens; Hi-C
library_construction_protocol: Immediately after sorting, cells were washed with PBS. Nuclei were extracted with cold lysis buffer and Tagmented with Illumina Tagment DNA Enzyme and Buffer Large kit (20034198). For RNA-seq, cells for RNA extraction were pelleted in Eppendorf tube, and resuspended with 350μl of Buffer RLT Plus containing 10ul of b-Mercaptoethanol (b-ME) per 1ml. For Hi-C, cells were crosslinked in a DNA low-bind Eppendorf tube at a final concentration of 2% formaldehyde and incubated at RT for 10 min on a tube revolver. Samples were then frozen in liquid nitrogen and stored in -80°C for future processing.