title: WGS of human CSF: Kp spikein 6
design: Pooled CSF was extracted using the QiaCube with Qiagen Blood and Tissue kit. Neat CSF extract was set aside for negative control. A. hydrophila and L. pneumphila (Components of RM 8376) were prepared and added to the bulk CSF extract to a concentration of ~1e4 genome copy/uL and 1.4e3 genome copy/uL, respectively. An aliquot was set aside. Individual dilutions series of each spike-in (E. faecalis, K. pneumoniae, L. monocytogenes, and N. meningitidis; all RM 8376 components) were prepared beginning with a 1:10 dilution of the stock (~1e7 genome copy/uL) for 6 total dilutions.