title: Metagenome of disinfectant stage of plant B
design: Suitable volumes of the water samples ranging from 100 ml for raw water to 1  for final treated water were filtered through 0.22 m polycarbonate membrane filters. The polycarbonate filters with collected microbial biomass was cut into small pieces using a sterile scalpel and used for DNA extraction. DNA extraction kits, MN - NucleoSpin Soil (50) 50 preps for the isolation of DNA from soil and sediment (Promolab Pty Ltd T/A Separations, RSA) were used following the manufacturers instructions. To account for potential biases introduced during DNA extraction, DNA was extracted in triplicates, and 5g genomic DNA used for further analysis was obtained by pooling the triplicates.