title: RNA-seq of mixed juvenile and adult apices
design: RNA from shoot apices with leaf primordia less than 3 mm were extracted using the Sigma Spectrum Plant Total RNA Kit using on-column DNase digestion (St. Louis, MO, USA). Each extraction consisted of a pool 5-10 apices from plants grown from the seed accession 19724 from the Australian Tree Seed Centre. For each extraction, apices were either from nodes 2-3, with leaf primordia having juvenile-compound morphology, or from nodes greater than 7, having adult-simple morphology. RNA concentration was measured using a Qubit Fluorometer (Life Technologies, Carlsbad, CA, USA) and a single pool of RNA was created using equal amounts of four juvenile extractions of juvenile and four adult extractions. The NEBNext Ultra II Directional RNA Library Prep Kit was used to construct a Poly A library according to the manufacture's protocol (New England Biolabs, Ipswich, MA, USA). The library was sequenced with PE150 format.