title: 16S rDNA amplicon seq of bacteria and archaea: rhizosphere soil
design: The genomic DNA of the rhizosphere sample (about 1 g) was extracted by CTAB or SDS method. Then, using a 1% agarose gel and the NanoDrop spectrophotometer 2000, DNA samples were examined for quality and quantity. After the purification of PCR products, TruSeq DNA PCR-Free sample preparation kit was used to construct the library. The constructed library was quantified by Qubit and Q-PCR. After the library was qualified, Illumina NovaSeq6000 platform was used for sequencing.