title: GSM7159167: Cut&Tag KO1 CARM1; Homo sapiens; OTHER
library_construction_protocol: hESCs were plated on dishes coated with Matrigel at a density of 10,000 cells per square centimeter. Then the hESCs were treated with CHIR99021 and h-BMP4 in N2B27 medium from day 0 to day 3. Next, cells were treated with h-PDGF-BB and ActivinA in N2B27 medium for VSMCs generation from day 4 to day 6. BACH1-KO hESCs were generated with CRISPR-Cas9 genome editing technology 80,000 cells were harvested and immobilized them in 10 μL ConA beads for 10 min at room temperature Sequencing libraries were prepared by using HyperactiveTM In-Situ ChIP Library Prep Kit for Illumina