title: GSM6423230: Group 3 innate lymphoid cell, Young gut  LC3s rep2, scRNAseq_PurfiedWTAIndex; Mus musculus; RNA-Seq
library_construction_protocol: Small intestinal ILC3s were sorted from siLP of 6-8 weeks-old C57BL/6 mice. All samples were uniformly resuscitated and washed twice by PBS (with 0.1% BSA), then the cells were 1 ml stain buffer (with 0.1% BSA) and counted. The viability of cells in all samples was assessed >80%. Cells for all samples were labeled with sample tags (BD Mouse Single-Cell Multiplexing Kit, USA), counted, and multiplexed, ready for single-cell capture. Single-cell capture and cDNA synthesis were performed by the BD Rhapsody Single-Cell Analysis System (BD, USA), according to the manufacturer's instructions.