title: RNAseq analysis of wild type Campylobacter jejuni compared to modE deletion strain
design: RNAseq analysis of wild type Campylobacter jejuni compared to modE deletion strain
library_construction_protocol: 5 ml of culture with an optical density at 600nm of approximately 0.6 was collected and centrifuged (15,000 g, 2 min, 4oC). The resulting supernatant was removed, and cell pellets were immediately frozen by submerging in liquid nitrogen for 10 seconds and transferred to a -80Â°C freezer. Campylobacter jejuni and the modE knockout were grown in defined medium (MEM) with replete Mo, W and Se to mid-log phase, then samples were taken for RNAseq analysis. RNA isolation was performed by GENEWIZ rRNA depletion and transcript library preparations were performed by GENEWIZ