title: Metagenomic Illumina sequencing of Wolbachia infected IDE8 tick cell culture (IDE8w20.1)
design: Illumina library preparation and sequencing of the samples were performed in the Centre for Genomic Research (CGR) at the University of Liverpool. NEBNext Ultra II FS kit paired-end libraries (2x150 bp) with a 350 bp insert were generated and sequenced with NovaSeq 6000 using SP chemistry. Raw fastq files were trimmed for the presence of Illumina adapter sequences using Cutadapt v1.2.1 with option -O 3; the reads were further trimmed using Sickle v1.200 with a minimum window quality score of 20; reads shorter than 20 bp after trimming were removed.