title: GSM7830185: Normal_K9me3; Mus musculus; ChIP-Seq
library_construction_protocol: Isolated primary retinal pigment epithelium cells were lysed in lysis buffer (0.5% Tween-20, 0.1% SDS, 0.5% NP-40, and Protease Inhibitor Cocktail) for 10 min on ice. Then MNase buffer (2 mM CaCl2, 100 mM Tris-HCl pH 8.0) containing 0.02 U MNase was added and incubated at 37°