title: GSM7782699: Breast Cancer, Visium CytAssist Spatial Gene Expression; Homo sapiens; OTHER
library_construction_protocol: For the Chromium Flex workflow, two 25 μm curls were pooled as a single replicate. 5 μm sections from Sample #1 were taken from the FFPE tissue using a microtome. Two replicate 5 μm sections were taken each for Visium CytAssist and Xenium. Corresponding dissociated tumor cells for Sample #1, fresh frozen (FF) in liquid nitrogen, were also sampled from the same 2.5 cm biopsy.  A 5 μm section was also taken from Sample #2 for Xenium. Library prep was performed according to 10x Genomics protocols.  5' GEX: https://www.10xgenomics.com/support/single-cell-immune-profiling/documentation/steps/library-prep/chromium-single-cell-5-reagent-kits-user-guide-v-2-chemistry-dual-index; 3' GEX: https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/library-prep/chromium-single-cell-3-reagent-kits-user-guide-v-3-1-chemistry-dual-index; scFFPE-seq (Flex): https://www.10xgenomics.com/support/single-cell-gene-expression-flex/documentation/steps/library-prep/chromium-single-cell-gene-expression-flex-reagent-kits-for-singleplexed-samples-optional-with-feature-barcode-technology-for-cell-surface-protein; Visium CytAssist: https://www.10xgenomics.com/support/spatial-gene-and-protein-expression/documentation/steps/library-construction/visium-cytassist-spatial-gene-and-protein-expression-reagent-kits;