title: GSM7622207: 7.5M 5xFADP522R mice; NeuN negative cells  [R2P_14]; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were isolated from the fresh-frozen cortical brain region as previously described (Hahn et al., 2021). NeuN-negative nuclei were separately sorted into 1.5 ml DNA LoBind tubes with 1 ml Dijon buffer. A total of 50,000 single nuclei were sorted from each sample. Nuclei were counted, and concentrations were adjusted to ∼1 million nuclei/ml. Reagents for the Chromium Single Cell 3ʹ Library & Gel Bead Kit v3 (10X Genomics). The nuclei/master mix solution was adjusted to target 10,000 nuclei per sample (5,000 nuclei from male mice and 5,000 nuclei from female mice) and loaded on a standard Chromium Controller (10X Genomics). Illumina sequencing of 10X snRNA-seq libraries was performed by Novogene Co. Inc.