title: GCM2 ChIP-seq biological replicate 2 in human parathyroid
design: Chromatin precipitation was performed on human parathyroid adenoma tissue using the EZ-Magna ChIP™ A kit (Millipore, Billerica, MA) as per the manufacturer's instructions. Tissue (5 mg) was crosslinked with formaldehyde, lysed, and sonicated to obtain 200-800 bp DNA fragments. Immunoprecipitation was carried out using specific antibodies, and magnet beads were used for separation and washing. Protein/DNA complexes were eluted and reverse crosslinked. Enrichment of DNA was assessed using SYBR-green Real-time PCR with GAPDH as a control.