title: 16S rRNA amplicon seq of sulfidic freshwater
design: V4-V5 hypervariable region of the 16S rRNA gene was amplified using 515F/926R primers modified with Illumina adapters on 5' end. PCR samples were barcoded with Illumina Nextera XT index 2 primers, purified with Millipore-Sigma MultiScreen Plate, purified with Qiagen PCR Purification Kit 28104 before sequencing on Illumina Miseq with the addition of 15-20% PhiX and 2x250 protocol.