title: parchment metagenomics
design: The DNA from the parchment fragment was extracted with the QIAGEN Powerlyzer Power Soil kit according to the manufacturer's instructions. The only alteration to the protocol were the three freeze/thaw (-80C/65C) cycles introduced after the C1 reagent is added to the sample. The concentration of the eluted DNA was determined by fluorometry and the DNA sent to a dedicated lab (STABVIDA) for metagenomics for both 16S (V3, V4) and ITS1.