title: Tissue sample of 16S rRNA from Amblyoma dissimile: Larva - Jul-21
design: 253 ticks (208 larvae, 21 nymphs and 4 adults) collected in vegetation were grouped in 27 pools, by month and by stage. For the preparation of the libraries, directed to the amplification of the V4 region of the 16S rRNA gene, the primers F515 and R806 were used. 1nM of the final library was sequenced by paired-ended v.2 chemical sequencing (150 bp reads) along with their multiplex sample adapters on the Illumina iSeq100 platform.