title: The effect of insulin-like growth factor 1 treatment on cardiac myeloid cells 3 days post-myocardial infarction
design: The effect of insulin-like growth factor 1 treatment on cardiac myeloid cells 3 days post-myocardial infarction
library_construction_protocol: Mice were exposed to 45 min ischemia followed by 3 days reperfusion.  During reperfusion mice were treated with IGF1 (bolus of 40 ng/g followed by 1 µg/g/d via osmotic minipumps) or vehicle (0.1%BSA). In each sample 1 control and 1 IGF1 treated mouse are combined. Hashtag labels indicate previous treatment (TotalSeq B0301 (Biolegend 155831) - control, TotalSeq B0302 (Biolegend 155833) - IGF1) After 3 days hearts were digested in a collagenase solution and CD45+Cd11b+ cells were isolated from the heart by FACS sorting.  Chromium Next GEM Single Cell 3ʹReagent Kits v3.1 Chromium Next GEM Single Cell 3ʹReagent Kits v3.1