title: D13_5
design: Total RNA was extracted from each individual using six biological triplicates for each developmental stage described above, using the Qiagen RNeasy kit. Ribosomal RNA (rRNA) was removed with the Epicentre Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI), following the manufacturer s protocol. A total of 60 libraries was constructed, and we performed paired-end sequencing (2  150 bp) using an Illumina HiSeq 2558 platform.