title: GSM8160866: Crypts, NT; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell RNA-sequencing (scRNA-seq) including paired intestinal crypt samples from NT and ES mice. Duodenal crypts were isolated through a meticulous procedure involving longitudinal opening of the duodenum, cold DPBS washing, and a 30-minute incubation with 5 mM EDTA on ice. Following this, crypts were mechanically separated from the intestinal tissue, filtered through a 70 µm mesh into a 50 mL tube for isolation from villus. To achieve a single-cell suspension, crypts were dissociated into individual cells using Tryple Express (GIBCO) for 5min and filtered through a 40 µm mesh. Subsequently, 5 animals per condition were pooled to minimize sample variation. The scRNA-seq libraries were generated using the 10X Genomics Chromium Controller Instrument and Chromium Single Cell 30 V3 Reagent Kits (10X Genomics, Pleasanton, CA) according to the manufacturer's recommendations. The experiments were performed by NovelBio Bio-Pharm Technology Co.,Ltd.