title: Illumina Seq of soil samples from the Sor Rondane Mountains (Antarctica)
design: PCRs targeting V4 region of the 18S rRNA gene was amplified using the general forward TAReuk454FWD1. PCR duplicates for each sample were pooled and subsequently purified with Agencourt AMPure XP beads (Beckman Coulter Inc.). Twelve to sixteen-cycle (depending on the amount of DNA) index PCRs using Nextera XT (Illumina Inc.) barcodes were performed as described in the Illumina 18S rRNA metagenomics library prep guide. The library prep was again purified using AMPure Beads XP (Beckman Coulter Inc.) as above. Amplicon libraries were sequenced on an Illumina Miseq platform (2  300 bp paired-end, v3 chemistry) at Genewiz (Azenta Life Sciences, Leipzig, Germany).