title: normal: Sample SU2CLC-DFC-DF0659-N1-DNA
design: DNA was extracted from FFPE tumor specimens and either matched normal whole blood, or in cases where this was unavailable, from adjacent normal FFPE specimens. Extraction was performed using the Qiagen AllPrep DNA/RNA Mini Kit (cat# 80204). A single aliquot of 150-500 ng input DNA in 100 μl TE buffer was used for library generation. Library preparation was performed using the Kapa HyperPrep kit, and quantification was performed using PicoGreen. Adapter ligation was performed using the TruSeq DNA exome kit from Illumina per manufacturer's instructions. Sequencing of pooled libraries was performed using a HiSeq 4000 with 76 bp paired end reads. Mean target coverage for tumor and normal samples were 150X and 80X, respectively.