title: 16S rRNA gene sequencing of rattus norvegicus:Intestinal contents of rats with primary membranous nephropathy treated with Cyclosporin A
design: DNA from different samples was extracted using the CTAB according to manufacturer s instructions. The full-length 16S rRNA gene was amplified using primers 27F: 5-AGRGTTTGATYNTGGCTCAG -3 and 1492R: 5- TASGGHTACCTTGTTASGACTT-3, which were tagged with specific barcode per sample. The PCR products were confirmed with 2% agarose gel electrophoresis, and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, USA) according to the manufacturers instructions. After quantified by QuantiFluorTM-ST ( Promega, USA)the amplicon pools were prepared for libraries construction. SMRTbell libraries were prepared using the Pacific Biosciences SMRTbellTM Template Prep kit 1.0 (PacBio, USA) and sequenced on PacBio RS II(LC-Bio Technology Co., Ltd., Hangzhou, China).