title: RNA-seq of Arabidopsis thaliana Tscha-1: Low N, shoot, rep2
design: Seventeen Arabidopsis accessions, Col-0, Ler, Bay-0, Bu-0, Da-0, Edi-0, Go-0, Ka-0, Lm-2, Sorbo, Tscha-1, Ullapool-8, Wc-2, Yeg-3, and Yeg-4, were grown on 1/2 MS agar plates for 5 days and then on modified 1/2 MS agar plates (N-free 1/2 MS salts, 0.8% agar, 0.5% sucrose, and 3 mM MES-KOH [pH 5.8] supplemented with 2 mM KNO3 and 2 mM NH4NO3 [6 mM N] or with 0.1 mM KNO3 and 0.1 mM NH4NO3 [0.3 mM N]) for 4 days. Shoot and root RNA samples were prepared using the Maxwell RSC Plant RNA Kit, and RNA-seq analysis was performed using Illumina Hiseq 2500.