title: GSM7829917: D0 GEX; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were harvested and homogenized through 100μm cell strainers to create single-cell suspensions. RBCs were lysed using Lysing Buffer Hybri-Max (Sigma-Aldrich). Samples were washed twice in PBS 2% BSA and resuspended in PBS 2% BSA containing propidium iodide at dilution of 1:500. Live cells were sorted using a BD FACS Aria III and loaded onto the 10X Genomics Chromium controller. cDNA libraries were prepared using Single Cell 3' Reagent Kits v4 (10x Genomics).