title: RNA-Seq of Linum usitatissimum: xylem tissues enriched in secondary cell walls, opposite side of the inclined stem at 1 hour of gravistimulation
design: Xylem tissues enriched in secondary cell walls collected from the same stem segment as phloem fibers (5 cm long stem portion, at the 3 cm above cotyledon leaves) from opposite side of the inclined stem at 1 hour of gravistimulation. The samples were fixed with liquid nitrogen and stored at 80C until RNA extraction. Total RNA was isolated from plant samples using the Trizol extraction method combined with RNeasy Plant Mini Kit (Qiagen) according to the manufacturers instructions. The quantity and quality of RNA was controlled using NanoPhotometer NP-80 Touch (Implen, Munich, Germany). RNA integrity test (all samples had a RIN value higher than 7), cDNA library construction, and sequencing were performed by the Novogene Corporation (China; https://en.novogene.com/). Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.