title: GSM8438852: F36P ERG, bio rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of 2 x10^6 cultured ERG overexpression or vector F36P cells in each sample was extracted by RNeasy Mini Kit (QIAGEN). mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.