title: RNA-Seq of wild type and paspro1/paspro2 mutant anthers
design: RNA-Seq of wild type and paspro1/paspro2 mutant anthers
library_construction_protocol: The samples were collected from paddy field at the anthesis stage. We froze the anthers immediately using liquid nitrogen.  Plants were transplanted to paddy field after two weeks in incubator at BOD incubator at end of May. The plants were then grown for 3 months in the field. We extract RNA from the sample using RNeasy Plant Mini Kit from QIAGEN following manufacturer's instruction.   TruSeq Stranded mRNA Sample Preparation Guide, TruSeq Stranded mRNA Reference Guide # 1000000040498 v00 with TruSeq Stranded mRNA LT Sample Prep Kit.