title: Illumina NovaSeq 6000 paired end sequencing; ChIPseq - The ETO2 scaffolding factor maintains acute leukemia by driving a MYB/EP300-dependent oncogenic program
design: ChIPseq - The ETO2 scaffolding factor maintains acute leukemia by driving a MYB/EP300-dependent oncogenic program
library_construction_protocol: After growth, cell was wash in PBS and then fixed in 2mM DSG (incubation 45min at RT). Cell was washed in PBS and then incubated in PBS supplemented with 1% PFA for 10min. Reaction was quenched by the addition of 0.125M (final concentration) of Glycine After cell lysis, nuclei was digested (by Micrococcal nuclease) and/or sonicated. Sheared chromatin was then used for immunoprecipitation. DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina).