title: GSM7623804: IgG Scramble-1; Mus musculus; RNA-Seq
library_construction_protocol: Each CNS was isolated per mouse. A cell suspension was generated by papain digestion, neutralization, and 70um filtration of cell extract. Cells were centrifuged, resuspended in 30% Percoll, centrifuged, and washed in PBS. Cells were sorted by flow cytometry based on a negative selection strategy for astrocytes. Libraries were prepared by the Broad Institute according to the ChIP-Seq protocol as described (Trombetta et al 2014).