title: RIC-KO
design: mTORC2-deficient human embryonic stem cell (hESC) lines have been generated by disrupting RICTOR gene that encodes the specific subunit of mTORC2 using CRISPR/cas9 and sgRNA targeting technology. Two WT control clones have been generated by CRISPR/cas9 without sgRNA targeting. Total RNA was extracted from the four cell lines at the same time.