title: SON GO-CaRT on IPC purified from GW17 cerebral cortex
design: Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 20 and GW16/17  using flourescence activated nuclei sorting (FANS).  Sorted nuclei were incubated with Fab fragments to block antibodies used in FANS and ~20,000 nuclei were  subjected to GO-CaRT using  antibodies to nuclear speckle scaffolding protein, SON. Sequencing libraries were constructed using KAPA Hyperprep kit following manufacturer's instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq 6000.