title: sRNAseq Arabidopsis + IR-CFA6/HRPL Apoplastic fluid SN fraction
design: Small RNA libraries were constructed and sequenced from SN fractions of IR-CFA6/HRPL#4 (Arabidopsis transgenic plants that constitutively express a chimeric inverted repeat carrying sequence homology with the coding regions of the coronaacic acid polyketide synthase I (cfa6) gene and of the hrpL alternative sigma factor of Pto DC3000). Supernatant (SN) fractions contain the lowest amount of canonical extracellular vesicles. It is collected by ultracentrifugation of the supernatant from P40 pellets at 100,000 g for 60 min at 4C. The supernatant collected at this step has been named SN fraction and is used for subsequent RNA extraction.