title: RNA-Seq of Dendrobium huoshanense: total plant
design: The ethanol precipitation protocol and CTAB-pBIOZOL reagent were used to purify total RNA from the whole plants of SPUF and GP according to the manufacturers instructions (Bioflux), and each sample was prepared with three biological replicates. RNA concentration and quality were measured using a Nano Drop and Agilent 2100 bioanalyzer (Thermo Fisher Scientific, MA, USA). After the RNA sample was qualified, fragment buffer was added to the enriched RNA to break the RNA into small fragments. Then, the first strand of cDNA was synthesized by reverse transcription with 6 bp random primers, and then the second strand was synthesized by adding buffer, dNTPs, DNA polymerase I and RNase H. Finally, cDNA library construction was performed by high-throughput sequencing using the Illumina HiSeq platforms at Novogene.