title: GSM8438591: MCF7_minusDOX_E2_ER-ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were grown in hormone-deprived media for 48 hrs before treatment for 45 min.  Crosslinking was then performed followed by chromatin extraction, sonication, and ChIP.  Crosslink reversal and chromatin isolation was performed before sequencing. Rubicon ThruPlex DNA-Seq library preparation system (publication number R400523, protocol QAM-108-002)