title: GSM8161122: Heart, 25bp variant MYBPC3 transgenic mice, RNA; Mus musculus; RNA-Seq
library_construction_protocol: Isolated hearts from NTG and TG mice were homogenized in 1 mL of TriZol (Bio-Rad, Hercules, CA) using a bead homogenizer, and total RNA was extracted using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Cat. No. 732-6820, Bio-Rad Laboratories, Hercules, CA), according to the manufacturer's instructions. mRNA in the total RNA was translated into a library of template molecules of known strand origin using the TruSeq® Stranded mRNA Library Prep kit (Cat. No. RS-122-2201, Illumina, Inc., San Diego, CA). Poly-T oligos attached to magnetic beads were used to isolate the mRNA, which was then fragmented and reverse transcribed. The resultant cDNA products were amplified, and cDNA libraries ready for sequencing were created using sequencing adapters and barcodes ligated onto the cDNA fragments for each respective sample.