title: RNA-seq of Daphnia magna grown in medium supplemented with Flumequine 2.0 mg/L (21-days-old females)
design: Total RNA was extracted starting from 3 daphnids using the Direct-zol RNA Microprep Kits, following the manufacturers instructions, and total concentration was determined using a Qubit RNA BR kit in a Qubit 2.0 Fluorometer. RNA quality was assessed with a 2100 Bioanalyzer; all the samples had an RNA Integrity Number (RIN) value > 6. A total of three replicates for each experimental group were processed. Nine tagged RNA-seq libraries were generated following the manufacturers protocols (BGI) and using a 100PE sequencing approach.