title: RNA-Seq of homo sapiens: plasma
design: 10 mL of blood was drawn from each subject into the Norgen cf-DNA/cf-RNA Preservative tube (#63950, Norgen Biotek, Canada) and centrifuged at 430 relative centrifugal force (rcf) for 20 minutes. The supernatant was collected giving 6 mL plasma per subject. RNA sequencing was conducted by BGI Hongkong Tech Solution NGS Lab (BGI Genomics, Hong Kong) using the Nugen low-input RNA sequencing protocol and the DNA Ball Sequencing (DNBSEQ) platform. Poly-A enrichment depletes undesired ribosomal RNA (rRNA), leaving purified circulating messenger RNA (mRNA). After sequencing, the data were filtered by removing the adaptor sequences, contamination (polyX; N content >= 1%; read length < 100 bp), and low-quality raw reads.