title: GSM7829194: Input; Mus musculus; ChIP-Seq
library_construction_protocol: Isolated E13.5 cortices were minced with blades in 1% Pierce formalin / PBS and further incubated ar room temperature for 10 min, followed by blocking with 1M glycine/PBS and washing with PBS twice. After adding SDS lysis buffer, the tissues were sonicated by Bioruptor. ChIP-seq libraries were prepared using the Takara ThruPLEX DNA-seq Kit. The libraries were size selected using AMPure XP beads, and validated using a Agilent TapeStation.