title: GSM7830706: Sample t53; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from glioma tissues was extracted using TRI Reagent (Sigma-Aldrich), and the quality was determined using the Qubit RNA IQ Assay Kit in a Qubit 4 Fluorometer (Thermo Fisher) according to the manufacturers' instructions. Only the RNA samples with integrity values > 6 were used for subsequent DNase treatment with the TURBO DNA-free Kit (Thermo Fisher) and retrotranscription into cDNA with the RevertAid First-Strand cDNA Synthesis Kit (Fermentas, Thermo Fisher). The starting amount of total RNA was 100 ng following Illumina's recommendations for preparing the sequencing library using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus.