title: GSM8368406: SMG organoids; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were removed from BME (R&D Systems) and digested with TrypLE (Thermo Fisher Scientific) for 5 minutes into single cells using glass Pasteur pipettes. The cells were then washed with ice-cold AdDMEM/F12 (Thermo Fisher Scientific). Cells were resuspended in culture medium supplemented with Y-27632 dihydrochloride (10 µM, Abmole Bioscience) and filtered into FACS tubes through a cell strainer (Falcon). Cells were co-stained with DAPI (Sigma-Aldrich) and DRAQ5 (Biostatus), and subjected to FACS sorting for live cells. FACS was performed on a BD Influx™ cell sorter (BD Biosciences). 4,000 live cells per condition were subjected to droplet-based scRNA-seq using the 10x Genomics platform. Libraries were prepared using the 10x Genomics Chromium 3' Gene Expression solution v3.1 and sequenced on a NovaSeq 6000 (Illumina).