title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of AML cells with MPI KO and FLT3 tyrosine kinase inhibition
design: RNA-seq of AML cells with MPI KO and FLT3 tyrosine kinase inhibition
library_construction_protocol: Cells were grown in culture and treated with AC220 (1nM) and mannose (100µM) for 24 or 72 hours as indicated RNA extracted with RNAeasy kit (Qiagen, 74106) Library preparation was performed with the dUTP directional kit NEBNext® Ultra8482 II Directional RNA Library Prep Kit for Illumina®