title: GSM6423052: RV free wall, sham-operated, P21, ATAC-seq, rep2; Rattus norvegicus; ATAC-seq
library_construction_protocol: Myocardium of RV free wall was rapidly removed from executed rat and flash frozen in liquid nitrogen, RNA was harvested using Trizol reagent. Nuclei was extracted from samples, and the nuclei pellet was resuspended in the Tn5 transposase reaction mix. The transposition reaction was incubated at 37°C for 30 min. A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. Equimolar Adapter1 and Adatper 2 were added after transposition, PCR was then performed to amplify the library. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed with Qubit.