title: AN18637-BA32
design: RNA samples were treated with TURBO DNase (Thermo Fisher Scientific, Waltham, MA, USA) to remove DNA following manufacturers protocol. rRNA depletion sequencing libraries were prepared by using QIAGEN Fastselect HMR. RNA sequencing library preparation uses NEBNext Ultra II RNA Library Preparation Kit for Illumina by following the manufacturers recommendations (NEB, Ipswich, MA, USA).