title: treatment with NIC or shRNA on neurogenic-rich (GW15-18) and gliogenic-rich (GW19-23) primary cortical tissues: Sample O7-C8
design: Primary cortical tissue corresponding to neurogenic-rich (GW15-18) and gliogenic-rich (GW19-23) time points were dissociated and treated either with NIC or shRNAs on consecutive days as above. Cells were harvested at day 5 and samples processed using the Parse Biosciences 100WTA kit according to the manufacturer's recommendation. This method involves a combinatorial and sequential barcoding of fixed cells or nuclei followed by generation of sublibraries. 8 sublibraries were prepared and tested with BioAnalyzer for quality.