title: GSM6659215: Corpus_T_PID00164; Mus musculus; RNA-Seq
library_construction_protocol: For single-cell RNA-seq analysis of dataset1 and dataset2, we performed Quartz-Seq2 as reported previously [Sasagawa et al., Genome Biology 19:29(2018), https://doi.org/10.1186/s13059-018-1407-3]. For the Quartz-Seq2 method, single-cell was aliquoted into the wells of a 384-well PCR plate with cell-sorter. These plates contain reverse transcription primers with different cell-barcodes in each well. For dataset1 and dataset2, v3.1 RT primers and v3.2a RT primers are used, respectively. In dataset1, ERCC spike mix I was included in the lysis buffer of the 384-well PCR plate. The collected single-cell lysis plates were stored at -80 ºC until the next whole-transcript amplification steps. The whole-transcript amplification steps and sequence library preparation were performed as described in previous paper [Sasagawa et al., Genome Biology 19:29(2018), https://doi.org/10.1186/s13059-018-1407-3]. The sequence library DNA was analyzed by the Agilent High Sensitivity DNA Kit (Agilent) and QuantiFluor® dsDNA System (Promega) to measure the exact DNA size length and DNA concentration. The sequence library DNA was then analyzed with NextSeq500 HOP 75 cycles kit v2.5.