title: RNA-seq of whole plants from tissue culture
design: RNA from whole, rooted in vitro grown clones of Acra3RX were extracted using the Sigma Spectrum Plant Total RNA Kit using on-column DNase digestion (St. Louis, MO). The NEBNext Ultra II Directional RNA Library Prep Kit was used to construct a Poly A library according to the manufacture's protocol (New England Biolabs). The library was sequenced with PE150 format.