title: RNA-Seq of Linum usitatissimum: phloem fibers at the later stage of tertiary cell wall deposition, the non-inclined plants (control) at 1 hour of gravistimulation
design: Phloem fibers at the later stage of tertiary cell wall deposition collected from 5 cm long stem portion, at the 3 cm above cotyledon leaves from the non-inclined plants (control) at 1 hour of gravistimulation. The samples were fixed with liquid nitrogen and stored at 80C until RNA extraction. Total RNA was isolated from plant samples using the Trizol extraction method combined with RNeasy Plant Mini Kit (Qiagen) according to the manufacturers instructions. The quantity and quality of RNA was controlled using NanoPhotometer NP-80 Touch (Implen, Munich, Germany). RNA integrity test (all samples had a RIN value higher than 7), cDNA library construction, and sequencing were performed by the Novogene Corporation (China; https://en.novogene.com/). Raw reads from run were filtered to remove adapter sequences, contamination and low-quality reads.