title: 16S Bacteria of mock HF_Sed_2
design: Enzyme: Thermo Scientific Phusion High-Fidelity PCR Master MixPrimer concentration: 0.4 MCycling and Tm:For 16S v4v5 = 25 cycles at 53C515F    GTG YCA GCM GCC GCG GTA A    411 bp926R    CCG YCA ATT YMT TTR AGT TT    411 bp