title: DNA-seq of soil fungi
design: Soil DNA was extracted using the PowerSoil DNA Isolation Kit (Qiagen, Crawley, UK), and DNA concentration was determined using a spectrophotometer. The V4 region of the prokaryotic 16S rRNA gene was amplified using primers 515F and 806R, and the fungal ITS2 region was amplified using primers gITS7 and ITS4). Primer 515F and gITS7 contained a 191 bp barcode unique to each sample for Miseq sequencing detection.