title: GSM7830314: Embryonic day 18.5 fetal mouse leg muscle RNA-seq KO rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the leg muscles of 18.5-day-old KO and WT mice during the embryonic stage. DNase I treatment was performed to remove genomic DNA from the total RNA. For library construction, we used a protocol as described before.(Zhou et al., 2015) The purified library products were evaluated using a Bioanalyzer (Agilent) and SYBR qPCR and sequenced on an Illumina Hi-seq 2000 sequencer