title: Invitro RNA seq of Xanthomonas perforans AL65 tssM mutant post 16 hours of growth in XVM2 medium
design: Total RNA was extracted using TRIzolTM (Thermo Fisher Scientific, Waltham, MA, USA) protocol. The wild type X. perforans AL65 and the tssM mutant strains were grown in XVM2 medium (10 mM sucrose, 10 mM fructose, 0.01 mM casamino acids, 10 mM (NH4)2SO4, 5 mM MgSO4, 0.16 mM K2HPO4, 0.32 mM KH2PO4, 20 mM NaCl, 1 mM CaCl2, 0.01 mM FeSO4, pH 6.7) with initial inoculum concentration of 10^5 cfu/ml. Both wild type and the mutant strains were grown at 28C with agitation. The bacterial cells were collected post growth in XVM2 medium at 8 h and 16 h post-inoculation.