title: OmniC of A. crassicarpa leaf tissue
design: A Dovetail Omni-C library was prepped by Cantata Bio (Scotts Valley, CA) following the manufactures protocol. In brief, chromatin was fixed in place with formaldehyde in the nucleus. Fixed chromatin was digested withDNaseI and then extracted, chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks werereversedand the DNA purified. Purified DNA was treated to remove biotin that was not internal to ligated fragments. Sequencing libraries were generated usingNEBNextUltra enzymes and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of each library.