title: ATAC-Seq of ATF4 fl/fl bone marrow LT-HSCs
design: 20000-50000 LT-HSC cells were sorted from the BM of mice and lysed in lysis buffer (10 mM Tris-HCl, pH 7.4, 3 mM MgCl2, 10 mM NaCl, 0.1% [vol/vol] IGPAL CA-630) for 10 min on ice. After centrifugation at 500  g for 5 min, the isolated nuclei were incubated with 50 L transposition reaction buffer (TD501-01, Vazyme) at 37 C for 30 min. Following tagmentation, the reaction was halted using VAHTS DNA Clean Beads, and the DNA was purified using the Illumina TruePrep DNA Library Prep Kit V2 to prepare the final library. The library was then subjected to paired-end high-throughput sequencing using the NovaSeq 6000  platform. The clean reads were aligned to the mouse genome (GRCm38) using the BWA package and peaks were called using the MACS2 package (v2.2.5), with a false discovery rate (FDR) cutoff of 0.05