title: PacBio HiFi reads used for Sprattus sprattus draft assembly
design: High-molecular weight DNA for PacBio long read sequencing was extracted using the Nanobind Tissue Big DNA Kit v1.0; Standard TissueRuptor Protocol. The genomic DNA was subsequently cleaned using the Pacific Bioscience Guidelines for Using a Salt:Chloroform Wash to Clean Up gDNA. DNA concentration was measured using the Qubit Broad Range kit (Thermo Fisher Scientific #Q32850), purity was checked by UV-absorbance, and fragment lengths were determined using the Genomic DNA 165 kb Kit (Agilent #FP-1002-0275) on the Femto Pulse System.