title: GSM6659279: mESC_Firre_Rescue_firre_induced_30min_dox_rep3_PROseq; Mus musculus; OTHER
library_construction_protocol: Nuclear RNA was extracted using TRIzol lysis reagent (Thermo Fisher) from purified nuclei described in Miller et al. 2015 and then enriched with biotin  in a nuclear run-on experiments as described in Mahat et al. 2016. PRO-seq libraries were prepared from 10-20 million mES nuclei and 1% drosophila S2 nuclei and made during the biotin enrichment process as described in Mahat et al. 2016.