title: WGS of Cryptococcus neoformans: Resulting progeny of cross between PMY2650 and PMY2649
design: DNA was extracted with MasterPure Yeast DNA Purification kit and cleaned up the Zymo Research Genomic Clean and Concentrator kit (following manufacturer's instructions) followed by quantification with PicoGreen. After quantifying the DNA with PicoGreen, samples were prepped for genomic sequencing using seqWell's plexWell 96 kit to prepare the libraries. Briefly, samples were individually bar-coded in sets of 96 using randomly inserted transposons, pooled, and then purified. Next, each pooled sample was bar-coded, enriched, and finally size-selected purified. Libraries were sequenced at Duke University's Sequencing and Genomic Technologies Facility on the NovaSeq 6000 S-Prime with 150 basepair paired end reads.