library_construction_protocol: Sugarcane cotton aphid 16S amplicon analysis was conducted using the qiime2 (2021.2) pipeline. Quality filtering was performed using Fastp (v0.19.6), and sequences were merged using Flash (V1.2.11). The DADA2 plugin was employed for denoising high-quality sequences, with chloroplast and mitochondrial sequences being removed from all samples. The naive Bayes classifier in qiime2, based on the bacteria: Silva138/16S_bacteria database, was utilized for species classification of ASVs (Amplicon Sequence Variants), with a confidence threshold of 0.19.