title: SN3_NMDAR_DRS
library_construction_protocol: Synaptoneurosomes were prepared as described by Kuzniewska et al. (2018). Isolates from 5 rats were pooled together and split into two samples (control and NMDAR-stimulated) to obtain enough material for Nanopore Direct RNA Sequencing (DRS). The in vitro stimulation of NMDA receptors was done as described by Kuzniewska et al. (2018). 3 Âµg of cap-enriched RNA was mixed with 200 ng of S. cerevisiae oligo(dT)-enriched RNA and 5 ng of poly(A) standards. It was then used to prepare libraries with a Direct RNA Sequencing Kit (SQK-RNA002) from Oxford Nanopore Technologies (ONT), following the manufacturer's instructions. Sequencing was performed using R9.4.1 RevD flow cells on a MinION device, controlled by MinKNOW software (ONT) as described by Bilska et al. (2020).