title: Simplified sequencing of Bighead carp (a primary freshwater aquaculture species in China)
design: 1. DNA extraction. The DNA of the sample was extracted using phenol/chloroform extraction method. 2. Restrictive endonucleases digest DNA. Select BsaXI enzyme as the restriction endonuclease for genomic DNA. 3. Joint connection. Connect Solexa specific ligands (slx-ad1 and slx-ad2) to the enzyme cleavage products mentioned above. 4. PCR amplification and recovery of target fragments. 5. Library quality control.