title: GSM8438704: patient 14; Homo sapiens; RNA-Seq
library_construction_protocol: Consecutive 10 μm sections were prepared from formalin-fixed paraffin embedded (FFPE) blocks and areas of relevant pathology were circled on one slide which had been stained with H&E. The identified areas were macrodissected from the slides and placed into AutoLys M Tubes (ThermoFisher) for deparaffinization. Sequential DNA and RNA isolation from the recovered tissue was performed on a KingFisher Flex using the Applied Biosystems MagMAX FFPE DNA/RNA Ultra Kit (ThermoFisher). RNA quality was assessed using a TapeStation 4200 (Agilent) and 50 nanograms of total RNA was used as input for library preparation using the SMARTer Stranded Total RNA-Seq Kit v2 (Takara Bio) according to the manufacturer's instructions. RNA-Seq Libraries were validated by capillary electrophoresis on a TapeStation 4200 (Agilent), pooled at equimolar concentrations, and sequenced with PE100 reads on an Illumina NovaSeq 6000, yielding ~50 million reads per sample on average.