title: 16S Archaea of mock LS2
design: Enzyme: Thermo Scientific Phusion High-Fidelity PCR Master MixPrimer concentration: 0.4 MCycling and Tm:For 16Sv4v5 Archae = 30 cycles at 65CArch519F    CAGCCGCCGCGGTAA    397 bpArch915R    GTGCTCCCCCGCCAATTCCT    397 bp