title: GSM7073839: SCC15, YBX1KO, DOXpos, rep 2 [RNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed and RNA was extracted using TRIzol (Thermo Fisher Scientific). The total RNA quantity was measured using Qubit RNA HS (Thermo Fisher Scientific). 500 ng of total RNA was used for library preparation according to standard protocols (QuantSeq 3'mRNA-seq FWD, Lexogen). Indexed libraries were pooled and sequenced on a NextSeq500 (Illumina). 5-15 million single-end 75 bp reads were generated per sample.