title: WGS of Escherichia coli: benzalkonium chloride evolution: Persistence control line 1
design: Genomic DNA integrity and concentration were determined and adjusted. DNA was fragmented, following adapter ligation, amplification and size selection according to proprietary methods of Eurofins Genomics. Library quality was confirmed by assessing fragment size distribution and concentration. The used protocol is based on the NEBNext Ultra II Directional DNA Library Prep Kit.