title: Temporal RNA-Seq of Lupinus albus Cluster Root T048
design: The sampling began (T0) on eight-day-old plants grown in P-deficient conditions. A total of eight 1 cm-long transitioning CR segments from four independently grown plants was sampled, at a distance of 1 cm from the primary root, in the upper part of the root system where LRs are transitioning to CR, every 12 h for 5 days, covering the entire rootlet developmental process (T0 to T132). As a control, 1 cm-long lateral root segments not transitioning to CR were collected. Four biological replications were produced for each experiment. Total RNA was extracted from all frozen samples using the Direct-zol RNA MiniPrep kit (Zymo Research) according to the manufacturers recommendations.