title: GSM7781874: hiPSC-neurospheroids, day-1, control, replicate 4; Homo sapiens; RNA-Seq
library_construction_protocol: Human neurospheroid samples were harvested from the bioreactor and sedimented by centrifugation at 300 xg, for 5 min. The supernatant was discarded and resulting cell pellet was washed twice with PBS, followed by additional centrifugation. Dry cell pellets were snap-frozen in liquid nitrogen. The frozen samples were shipped on dry ice to Genewiz (Leipzig, Germany) for RNA sequencing analysis. The RNA sequencing workflow after RNA isolation included initial PolyA selection-based mRNA enrichment, mRNA fragmentation, and random priming were performed. First- and second-strand complementary cDNA synthesis and end-repair 5′ phosphorylation and adenine nucleotide (dA)–tailing were performed.