title: HiFi WGS of inbred mouse strain SARC/NachJ
design: This PacBio HiFi library was constructed using the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences) according to the manufacturers protocols.  Briefly, the protocol entails shearing the DNA using the g-TUBE (Covaris), ligating PacBio specific barcoded adapters, and size selection on the Blue Pippin (Sage Science).  The quality and concentration of the libraries were assessed using the Femto Pulse Genomic DNA 165 kb Kit (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively, according to the manufacturers instructions. The library was sequenced on a single SMRT cell on teh Sequel II platform (Pacific Biosciences) using a 30 hour movie time. The fastq file provides circular consensus reads.