title: GSM8368401: tub_3h; Mus musculus; RNA-Seq
library_construction_protocol: Cells were washed with 1xPBS and dettached with 0.05% trypsin in EDTA for 2 minutes at 37C, followed by quenching with complete growth media. Proper viability of cells prior to freezing was quantified with propidium iodide/acridine orange staining in a LUNA-FL Dual Fluorescence Cell Counter. Cells were centrifuged at 200rcf for 3 minutes. The supernatant was discaded and the pellet was resuspended in 0.5mL 0.04% BSA in PBS. Single-cell RNA barcoding (10x Genomics' Chromium) and sequencing (NextSeq 2000 instrument, Single Cell 3' v3 chemistry) was performed from fresh cells according to standard procedures.