title: GSM7234212: SC_D7_3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rats were anesthetized with 2.5% isoflurane/oxygen.  Right L4-L6 spinal coad was taken from each rat, and the samples were frozen in liquid nitrogen within 3 minutes after leaving the body.  The frozen samples were crushed and dissolved in TRIzol (Invitrogen).  RNA was subsequently extracted from tissues and the RNA were subjected to strict quality control (Agilent 2100 bioanalyzer). The reference genome is from the ensembl database (rattus_norvegicus_Ensembl_97).  Index of the reference genome was built using Bowtie v2.0.6 and paired-end clean reads were aligned to the reference genome using TopHat v2.0.9.  After the completion of the library construction, Qubit 2.0 was used for preliminary quantification, diluting the library to 1.5ng/ul, and then Agilent 2100 was used to detect the Insert size of the library to ensure the quality of the library.  After qualified library inspection, Illumina PE150 sequencing was conducted by pooling according to effective concentration of library and data output requirements.