title: GSM7999317: Hi-TrAC_IL4reporter_GFP+_rep2; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was purified by Phenol-Chloroform extraction. Gaps in purified genomic DNA were repaired with T4 DNA polymerase. Remove free bridging linker with AMPure XP beads. Digest purified DNA with restriction enzymes MluCI/NlaIII, and then enrich biotin-labeled DNA fragments with streptavidin beads. Perform adapter ligation on beads. After washing the beads, run PCR with illumina multiplexing indexed primers.