title: Scrambled control on GW22 cortical tissues: Sample S236-D5
design: Primary cortical samples were sliced and micro-dissected into germinal zone (GZ) and cortical plate (CP). Dissociated neural cultures were treated with lentiviral vectors carrying shRNA, directed against nAChRs, a Scrambled control or broad agonists (ACh, NIC). Cells were collected on Day 5 in culture, total RNA extracted and processed for Bulk-RNA-sequencing. For Bulk-RNA seq, samples were tested for RNA integrity using a BioAnalyzer. Only samples with a RIN score > 9.0 were sent for Bulk-RNA sequencing