title: DNA-seq of  two Aedes aegypti lines differing in resistance to deltamethrin
design: DNA-seq of  two Aedes aegypti lines differing in resistance to deltamethrin
library_construction_protocol: pool of 75 females, three days post emergence, not blood fed Gentra Puregene Tissue kit (Qiagen) with 3mL cell lysis bufferr.DNA fragment length was visualized on agarose gels, and concentration was measured with Qubit and adjusted to 150 ng/Âµl. DNA was size selected with SRE (Short Read Eliminator) kits from Circulomics. A library was prepared using 1 ug DNA and Nanopore SQK-LSK109 kit A library was prepared using 1 ug DNA and Nanopore SQK-LSK109 kit