title: BES metagenome
design: V4 of 16S rRNA gene. Primers 515F-806R Dual indexed Illumina compatible libraries produced in a single step. Primary PCR performed using fusion primers Fluidigm CS oligos at their 5' ends. The genomic DNA samples were sequenced using a Illumina MiSeq flow cell (v2) and a 500 cycle reagent kit (2 x 250 bp paired end reads).