title: GSM7697938: female MEFs, IgG CUT & RUN, rep1; Mus musculus; OTHER
library_construction_protocol: The CUT & RUN assay was performed as described in (Skene, Henikoff and Henikoff 2018), with minor adaptations. Briefly, 1 million MEFs were employed per condition and optimal digitonin (Enzo Life Sciences, UK) concentration for membrane  permeabilization was determined at 0.02 %. Primary antibodies (mouse anti-HP1γ and mouse anti-IgG included in the same set, Millipore #17-646; or mouse anti-H3K27me3, Abcam, #6002 were used at a 1:100 dilution and for spike-in purposes 100 pg of yeast DNA was employed DNA libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (New England BioLabs) following the manufacturer's instructions