title: GSM7783232: db.db control diet; Mus musculus; RNA-Seq
library_construction_protocol: The flesh whole kidneys in each mice group were isolated respectively after perfused with DBPS from left ventricle. The extracted kidneys were immediately placed in an ice-cold DPBS environment. The kidneys were then meticulously cut into roughly 1 mm 3 cubes using a sterile surgical blade. The minced tissue was digested with 4mL of digestion buffer containing 3.0 mg/ml Collagenase type II, 1.5mg/ml Pronase E , 62.5 U/mL DNAse, and 5mM calcium chloride made up in DPBS(-). The digestion mixture was incubated at 37℃ in a water bath for 20 minutes. The remaining erythrocytes were removed with RBC lysis buffer. The debris and dead cell in the cells were removed with Debris removal solution kit, and Dead cell removal kit respectively. The cells were resuspended with PBS/BSA 0.04% after removing the debris and the dead cells. Library was performed according to manufacters's instruncion (10x Genomics)