title: NGS sequencing of spacers from 1:1 competition of NAGG versus AGG spacer libraries during NM44 infection. Spacers are in the modified S. pyogenes type II-A CRISPR-Cas locus in S. aureus RN4220.
design: Barcoded PCR of the CRISPR array, followed by amplicon size selection by agarose gel and illumina library preparation