title: GSM8332940: MDA-MB-231, Input for HA-RCOR2, rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator (Brandson). ChIP-seq libraries were prepared using the NEBNext UltraII DNA Library Prep Kit (NEB). 1 ng of DNA was used as starting material for input and ip samples. Libraries were amplified 13 cycles on the thermocycler. Post amplification libraries were gel-purified for size selection at 300-500bp. Libraries were validated using Tapestation (Agilent) and quantified using Qubit Fluorometer (Invitrogen).