title: endu-2_HS+RE_1
design: Day 1 adult of animals raised at 20C from the L1 larval stage were used for total RNA isolation with RNeasy Mini Kit (QIAGEN). Purification of poly-A containing RNA molecules, RNA fragmentation, strand-specific random primed cDNA library preparation and 150 bp paired-end reads were carried out on an Illumina HiSeq 4000 by Eurofins Genomics.