title: mitogenome of Canis lupus: ancient/historical samples
design: aDNA extracts were used to prepare double-indexed libraries following the Meyer & Kircher (2010) protocol with few modifications. DNA libraries were enriched for mitochondrial genome using home-made baits and the protocol from Horn (2012). Enriched libraries were pooled, quantified using NEBNext Library Quant Kit for Illumina (NEB) and sequenced on NextSeq 500 (Illumina) using three NextSeq 500/550 Mid Output v2.5 (2x75 cycles) kits