title: 16S amplicon NGS_Cell 5-6 Water end   0 mV Step 2
design: The NGS was performed at Stab Vida Lda (Lisbon, Portugal). Sequencing targeted the V3 and V4 regions of the bacterial 16S rRNA gene using primers 341F (CCTACGGGNGGCWGCAG) and 785R (GACTACHVGGGTATCTAATCC) and producing an amplicon size of 444 bp (Klindworth et al., 2013). The generated DNA libraries were sequenced with MiSeq Reagent Kit Nano in the lllumina MiSeq platform, using 300 bp paired-end sequencing reads. The generated nucleotide sequences are available at the NCBI SRA repository.