title: Oxford Nanopore genome sequencing of Teredinibacter turnerae: strain SR02026
design: Genomic DNA was extracted from the resulting clonal isolates using the Qiagen DNeasy Blood and Tissue Kit following the manufacturers recommended protocol for cultured cells with the exception that DNA was eluted with two 75 L volumes of AE buffer preheated to 56 C. DNA quality and length were assessed on Tapestation (Agilent Technologies, US). Nanopore (Oxford Nanopore Technologies, UK) sequencing was performed without DNA fragmentation. The sequencing library was prepared using the Q20+ chemistry Ligation Sequencing Kit (SQK-LSK112) and sequenced on a MinION (Mk1B) instrument using a R10.4 (FLO-MIN112) flow cell. Bases were called using Guppy (v6.4.6) high accuracy (config dna_r10.4_e8.1_hac.cfg) algorithm and default quality filtering.