title: GSM8033111: HeLa, TAF2, CUT&RUN, IgG control; Homo sapiens; ChIP-Seq
library_construction_protocol: Standard CUT&RUN according to Skene et al., 2018 NEB Next Ultra II library preparation kit (NEB #E7645L) without size selection according to manufacturer's instructions with modifications: we increased the end prep duration to 60 min at 50 °C instead of 30 min at 65 °C and used only 10 s annealing and extension time instead of 75 s, and 15 PCR cycles during the amplification step