title: RNAseq of HepG2: rep3
design: RNA was extracted from 15 l of cell lysate using the Direct-zol RNA Microprep Kit (Zymo Research) following the manufacturers instructions and including DNAse treatment for 15 min at room temperature. Samples were eluted with 15 l nuclease-free water. The RNA integrity numbers (RIN) of the samples were between 9.9 and 10.0, measured using High Sensitivity RNA ScreenTape (TapeStation system, Agilent). RNA was quantified using a Qubit fluorometer. Libraries were prepared using the SMART-seq Stranded for total RNA-seq kit (Takara) from 5 ng of RNA and sequenced on the Illumina NextSeq 500 sequencer in the Genomics Facility Basel (Department of Biosystems Science and Engineering (D-BSSE), ETH Zrich).