title: GSM8161054: ventricular cells, myd88-/-, 24 hpci; Danio rerio; RNA-Seq
library_construction_protocol: Ventricular cells were isolated from a pool of 4 myd88+/+ and 4 myd88-/- ventricles for the myd88+/+ and myd88-/- sample, respectively. Cell isolation was performed with the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher Scientific) following the manufacturer's instructions and with the following modifications: Incubation was performed at 30°C for 20 min, followed by resuspension in 1× Hanks' balanced salt solution (Gibco) with 0.25% BSA. Suspended cells were sorted using FACSAriaIII sorter (BD) to exclude dead cells using DAPI (Sigma). Library preparation was conducted according to the manufacturer indications (Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1, 16 rxns PN-1000121).