title: single cell RNA-seq of human PBMCs
design: The scRNA-seq samples were processed using the in-house pipeline. Illumina base call files for all libraries were converted to FASTQs using bcl2fastq v2.20.0.422 (Illumina) and FASTQ files associated with the gene expression libraries were aligned to the GRCh38 reference assembly with v32 annotations from GENCODE (10x Genomics GRCh38 reference 2020-A) using the version 6.1.2 Cell Ranger multi pipeline (10x Genomics). The generated gene - cell expression matrix was considered for the downstream analysis.