title: Silvatares_Illumina
design: A total of 110 ng gDNA was sheared to a mean fragment size of about 420 bp using a Bioruptor Pico (Diagenode, Seraing, Belgium). Genomic libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit for Illumina (New England Biolabs, Ipswich, MA, U.S.A.) according to the manufacturers manual. Adapters were diluted 1:10 as recommended for low input libraries, and size selection was conducted based on the insert size using SPRIselect beads (Beckman, Indianapolis, U.S.A.). A dual indexing PCR was run for 8 cycles on a Mastercycler (Eppendorf, Germany). After cleanup, the library was eluted in 0.1X TE, and shippedfor 150 bp paired-end readssequencing on a partial lane of an Illumina NovaSeq 6000 platform (San Diego, CA) at Novogene (Cambridge, UK).