title: PacBio HiFi of A. crassicarpa leaf tissue
design: HMW DNA was extracted from young leaf primordia and shoot apices of an adult clone of Acra3RX using an in-house CTAB protocol at Cantata Bio (Scotts Valley, CA). DNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). The PacBioSMRTbelllibrary (~20kb) for PacBio Sequel was constructed usingSMRTbellExpress Template Prep Kit 2.0 (PacBio, Menlo Park, CA, USA) using the manufacturer recommended protocol. The library was bound to polymerase using the Sequel II Binding Kit 2.0 (PacBio) and loaded onto PacBio Sequel II). Sequencing was performed on PacBio Sequel II 8M SMRT cells.