title: GSM7864411: Control_4_RNASeq; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from GFP positive portions of brains of 5 control and 5 Gcm1 overexpressed mice at E16.5 using RNeasy Plus Universal Kit (Qiagen). Quality and quantity measurements of extracted RNA were performed using NanoDrop (Thermo Fisher Scientific) and Qubit Fluorometer (Thermo Fisher Scientific), respectively. Sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for directional libraries (New England BioLabs) and the KAPA HTP Library Preparation Kits (KAPA Biosystems) according to the manufacturer's instructions. The RNA-seq libraries were each sequenced (101 cycles) using the Illumina HiSeq platforms.