title: GSM8438549: Muscle, KPC, FoxP1 skeletal muscle KO, 04:00/circadian time 22, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was performed by homogenization of tibialis anterior muscles in Trizol followed by extraction with chloroform and precipitation with isopropanol. Isolated RNA was sent to Novogene Co., LTD (Sacramento, CA) for quality assessment, cDNA library construction, and Illumina transcriptome sequencing aligned to the mouse genome (mm39). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.