title: GSM8332901: CAMA, Untreated, biol rep2; Homo sapiens; RNA-Seq
library_construction_protocol: After the end of the treatment, each dish was washed twice with PBS, then a scraper was made in the center of the dish and recovered in 200 μl of PBS. 400 μl of Lysis/binding Buffer from the Roche “High pure RNA isolation Kit (11828665001)” kit was added, and the solution was frozen at −80°. The rest of the extraction was performed according to the protocol of the previously mentioned Roche kit. RNA concentration was measured with the NanoDrop One. Library preparation has been done with 400ng of input RNA using: NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) followed by NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (NEB# E6440).