title: RNA-seq of Ficus glaberrima: leaf tissue
design: mRNAs are isolated from total RNA with oligo(dT) method. Then the mRNAs are fragmented. The first and second strand cDNA are synthesized. cDNA fragments are purified and resolved with EB buffer for end preparation and single nucleotide A (adenine) addition. After that, the cDNA fragments are linked with adapters. Those cDNA fragments with suitable size are selected for the PCR amplification.