title: GSM7074875: T111; Homo sapiens; RNA-Seq
library_construction_protocol: Resected patient tumors were dissociated followed by red blood cell lysis, washed, and passed through 22µm sterile filters to obtain single-cell suspensions. Dead cells were excluded by SYTOX staining and live cells were collected by the FACSAria Fusion flow cytometer (BD Biosciences) and BD FACSDiva (v8.0.1). Sorted cells were washed with PBS containing 0.04% BSA and resuspended at a final density of ~1000 cells/µL. Libraries were created targeting ~10,000 cells/sample for capture using Chromium Single Cell 3' Reagent Kits following the CG00052 Rev B. user guide. Each library was subsequently sequenced on the Illumina NovaSeq 6000 platform to targeting an average of ~50,000 reads/cell.