title: RAN-seq from LP PARIS IN DBP 10-8 M  Rep2
design: Prior to RNAseq analysis, the total RNA from 24h-old cultures of L. pneumophila Paris  grown in presence of 10-6, 10-8 M PAEs or 0.1% DMSO was extracted with Trizol as  previously described in Verdon, J. et al (2011).Total RNA was quantified in the samples with a NanoDrop  One (Thermo Scientific).The RNA integrity was verified by using an automated  electrophoresis system (MultiNA Bioanalyzer MCE-202, Shimadzu).The RNA samples were then sent to a platform (Institut du Cerveau et de la Moelle Epinire, Paris, France; https://icm-institute.org/fr/) for ribodepletion, library preparation and  sequencing using an Illumina NovaSeq 6000 paired-end 2150-bp technology.