title: Targeted genetic therapy for congenital melanocytic naevi
design: Targeted genetic therapy for congenital melanocytic naevi
library_construction_protocol: HCT116 cells were grown in vitro before treatment with siRNA for 48 hours: 7.5ul RNAiMAX / 25 pmol siRNA / 9.6cm2 well RNA was extracted using the RNAeasy plus mini kit (Qiagen, Germany), using RLT buffer to lyse cells, 70-90% ethanol to precipitate the nucleic acids and nuclease free water to resuspend the RNA. 24 samples, prepped with the KAPA mRNA HyperPrep kit (KK8580)