title: GSM8332839: Reconstituted chimeric follicle (RCF) AA, biol rep 11 [S278]; Mus musculus; RNA-Seq
library_construction_protocol: Single oocytes were collected with NEBNext Single Cell Lysis Module (NEB #E5530S) in 8μl volume and flash-frozen and stored at -80°C. NEBNext Single Cell/Low Input RNA Library Prep Kit (NEB #E6420) was used for cDNA synthesis, amplification and library generation. Manufacturer's protocol for cells was followed with these specifications: 16 PCR cycles for cDNA amplification, 8 PCR cycles for enrichment of adaptor-ligated DNA with unique dual index primer pairs from NEB.