title: MeDIP-seq input of CSA high binding P. falciparum NF54 parasites
design: Infected RBCs were collected at 16 h.p.i. and lysed using 0.1% saponin (Sigma). Genomic DNA was extracted using QIAamp DNA Blood Kit (Qiagen) with RNAse A treatment. gDNA was fragmented using the Covaris ME220 ultra sonicator. Libraries were generated using the KAPA LTP library preparation kit (KAPA Biosystems). Prior to immunoprecipitation, a small aliquot was made to use as an input control.. Samples were purified using 1X KAPA Pure beads (KAPA Biosystems) and PCR amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems).