title: RNA-Seq of homo sapiens: adult female breast cancer
design: Total RNA was isolated using a GeneJET RNA Purification Kit (Thermol) according to the manufacturers protocol and 1 g of RNA was converted to cDNA using the Hifair III 1st Strand cDNA Synthesis SuperMix (Yeasen) in the presence of both oligo (dT) and random primers. RNA integrity was assessed using the RNA Nano 6000 Assay Kit on the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). For sequencing, samples with specific adaptors were sequenced on an Illumina NovaSeq platform, and 150 bp paired-end reads were generated according to the manufacturers instructions.