title: Amplicon-Seq of Wastewater Influent: Treatment Plant
design: 24-hour composite wastewater influent collected from a wastewater treatment plant in Peel, Ontario, Canada. Microbes were isolated using Ceres Nanotrap Microbiome A isolation beads, RNA was extracted and PCR-amplifed with primers specific to the Tomato Brown Roguse Fruit Virus genome sequence. PCR amplicons were prepared for Illumina sequencing using the Illumina DNA Library prep kit.