title: GSM6658646: nup211-so, no treatment control, biol rep 2 [Sample_2]; Schizosaccharomyces pombe; RNA-Seq
library_construction_protocol: Fission yeast cell walls were digested using 250 µg of lyticase (Sigma) for 1.0-2.5 hours at 30°C. Total RNAs were extracted from the cells using the RNeasy Kit (Qiagen). RNA libraries for RNA-seq were prepared using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) according to the manufacturer's instructions