title: GSM6533834: OP50-rep4; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Bacteria were removed and worms were natural precipitation by M9 buffer , flash frozen on dry ice, and RNA was harvested using Trizol reagent.A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. RNA libraries were prepared for sequencing using standard Illumina protocols