title: RNA-Seq of Eulimnogammarus verrucosus from Ust-Barguzin, field sample, 20181
design: Sample was homogenized in 1 ml Qiazol reagent (Qiagen) using a MM400 mixer mill (Retsch). RNA was extracted from whole-body samples using the RNeasy Mini kit (Qiagen) and treated with DNase (TURBO DNA-free Kit, Thermo Fisher Scientific) according to the manufacturer's instructions. mRNA was separated from total RNA using the Oligotex mRNA Mini Kit (Qiagen). RNA concentration and quality were evaluated using a Qubit 2.0 fluorometer (Thermo Fisher Scientific) and a 2100 Bioanalyser instrument (Agilent). The library was prepared using the NEBnext Ultra II Directional Library Preparation Kit following the standard protocol for mRNA enrichment with Poly-A selection module (New England Biolabs Ltd.). Paired-end (PE) sequencing was performed with an Illumina HiSeq 2000 device (Illumina Inc.), yielding sequences with a read length of 100 bp. 24 RNA-seq libraries (from amphipods from a different experiment not analysed in this study) were pooled and sequenced on four lanes of a v3 PE HiSeq flow cell.