title: GSM8368767: PEDV_SHAPE_cell_rep1; Porcine epidemic diarrhea virus; OTHER
library_construction_protocol: At 12 h post-infection, infected cells were washed 3 times with 10 ml cold PBS and then resuspended in PBS containing NAI at a final concentration of 100 mM. Another sample was added with an equal amount of DMSO as a control. Samples were then incubated at room temperature with continuous shaking for 15 minutes, after which the reaction was terminated by the addition of DTT at a final concentration of 0.5 M. The supernatant was then removed and the cells were lysed by adding 6 ml of Trizol. Extract the RNA by adding 1.2 ml of chloroform:isoamyl alcohol (24:1). Sequencing libraries were generated using a VAHTS® Universal V8 RNA-seq Library Prep Kit For MGI (Vazyme #NRM605), according to manufacturer's protocol. Libraries concentration were measured using a Qubit dsDNA HS Assay Kit (ThermoFisher, Cat. No. Q32851). 1 μl of the final library product was taken and the library quality was analysed using the Agilent DNA1000 Kit (Agilent, Cat. No. 5067-1504).