title: Illumina NovaSeq 6000 paired end sequencing; Gene Expression profiling by RNA-seq of pig intestine segments tissus and their derived organoids. RNA-seq data was generated to assess to what extent porcine gut organoids derived from different segments of the small intestine (duodenum, jejunum, ileum) and colon recapitulate the phenotypic variability of the tissue they are derived from, with a focus on phenotypes involving gut innate immunity responses.
design: Gene Expression profiling by RNA-seq on an Illumina Novaseq 6000 for functional annotation analysis. RNA-seq data of pig intestine segments tissus and their derived organoids were generated to assess to what extent porcine gut organoids derived from different segments of the small intestine (duodenum, jejunum, ileum) and colon recapitulate the phenotypic variability of the tissue they are derived from, with a focus on phenotypes involving gut innate immunity responses. This study is part of the FAANG project, promoting rapid prepublication of data to support the research community. These data are released under Fort Lauderdale principles, as confirmed in the Toronto Statement (Toronto International Data Release Workshop. Birney et al. 2009. Pre-publication data sharing. Nature 461:168-170). Any use of this dataset must abide by the FAANG data sharing principles. Data producers reserve the right to make the first publication of a global analysis of this data. If you are unsure if you are allowed to publish on this dataset, please contact the FAANG Data Coordination Centre and FAANG Consortium (email faang-dcc@ebi.ac.uk and copy faang@iastate.edu) to enquire. The full guidelines can be found at http://www.faang.org/data-share-principle.
library_construction_protocol: ILLUMINA stranded mRNA prep