title: NGS SYK-6 RB-TnSeq library: Wx-SEMP + 500 uM erythro-DGPD enrichment
design: 3 x 0.15 mL cell pellets were pooled and used to extract and purify gDNA. Specific primers amplified the transposon-associated barcode region with Q5 High-Fidelity 2X Master Mix (New England Biolabs, USA). Samples were pooled and sent to Genewiz for sequencing.