title: Bali honey
design: The fungal ITS1 region was amplified using the primers BITS-ACCTGCGGARGGATCA and B58S3-GAGATCCRTTGYTRAAAGTT (Bokulich and Mills 2013). An additional 5 bases of inline barcode wereincorporated at the 5 end of the primers to enable inline barcoding (Glenn et al. 2019). Different samples were amplified using different combinations of the forward and reverse inline primers. PCR was performed using SolarBio PCR mastermix (SolarBio, China) with the PCR profile of : 95C for 3 minutes followed by 35 cycles of 95C for 15 s, 48C for 20s and 72C for 10s. The barcoded amplicons were subsequently visualized on gel and purified using 0.8 X of SPRI bead. The purified amplicons were used as the template for 8 cycles of index PCR to incorporate the complete Illumina adapter and Illumina-compatible dual-index barcodes. The constructed libraries were subsequently size-selected using 0.8 X vol of SPRI bead and pooled into a single tube. Quantification of the pooled libraries used Denovix high sensitivity assay. Sequencing of thepooled libraries was performed on a NovaSEQ6000 (Illumina, San Diego) using the 2 x 150 bp paired-endsequencing configuration.