title: S. alvi +E2C
design: High molecular weight gDNA was isolated using the Quick-DNA HMW MagBead kit (Zymo Research, USA). 175 ng of gDNA was prepared using a Rapid Barcoding Sequencing Kit and sequenced on a R9.4.1 flow cell using a MinION MK1C instrument (Oxford Nanopore, UK). Basecalling was done using Guppy (v6.3.9) in fast mode.