title: genome sequence of an Escherichia coli phage phiD2-2
design: the DNA sample was fragmented by sonication to a size of 350bp, then DNA fragments were end-polished, Atailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analysed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.