title: Fungal ITS2: plant roots
design: 2 x 250 bp paired end reads. Amplicons generated using fungal ITS2 primers ITS4-fun (Read 1) and 5.8S-fun (Read 2) from Taylor et al (2016; https://doi.org/10.1128/AEM.02576-16), in conjunction with the Ilumina Nextera kit.