title: DNA Sequence of Vernonia Amygdalina Chloroplast
design: Fragmentation - NEBNext Ultra II FS DNA Library Prep Kit for Illumina, DNA fragment selection(>200bp) AMPure XP beads, fragments were repaired ligated to illumnia adapter sequences, size selected quantified(NEBNext library Quant Kit for illumnia), diluted to 4nM then sequenced