title: sRNAseq Arabidopsis + IR-CFA6/HRPL Apoplastic fluid p40 fraction
design: Small RNA libraries were constructed and sequenced from P40 pellets of IR-CFA6/HRPL#4 (Arabidopsis transgenic plants that constitutively express a chimeric inverted repeat carrying sequence homology with the coding regions of the coronaacic acid polyketide synthase I (cfa6) gene and of the hrpL alternative sigma factor of Pto DC3000). P40 pellets were purified by subjecting apoplactic fluids to an ultracentrifugation step at 40,000 g for 60 min at 4C. P40 pellets were next Mnase treated for 15min at 37C before RNA extraction