title: RNA-seq of Hydrangea: flowering-F13
design: We obtained sequencing RNA to generate cDNA libraries in order to assess the molecular mechanisms involved in color shifting and floral scent formation during different flower development stages. Supplementary Table S2-4 include detailed information about sequencing and de novo assembly. Transcriptome sequencing yielded 6.20GB-7.62GB bases with Q30 and Q20 values greater than 93.68 and 97.87 %, respectively (Supplementary Table S2). Among the high-quality clean reads acquired, the F1 stage has the most (45351981), followed by the F3 (42385164) and F2 stages of flower development (44288070). The average clean data percentage was greater than 94.1 % (Supplementary Table S3). The nuclear genome sequence of the H. arborescens Annabelle was unavailable; therefore, Trinity package was employed to assemble the clean reads into unigenes. A total of 108610807 unigenes with a GC content of 40.32 % and a N50 length of 1198 bp were retrieved. The de novo assembly generated, 122490 sequence numbers with a maximum length of 16528 bp and an average length of 886.69 bp (Supplementary Table S4). Briefly, the finding implies that the sequences were of high quality and fulfilled the criteria for further investigation.