title: NEBNext Ultra II DNA library from Arabidopsis thaliana DNA (TP (#4) single plant with tae phenotype (tae BC7), that were crossed with Bla parent (BP) to produce the F2 population (8t)) sequenced on Illumina NextSeq 550 with NextSeq 500/550 High Output Kit v2.5.
design: DNA from young rosette leaves was extracted using DiamondDNA plant kit (Russia), according to the manufacture's protocol. DNAs were fragmented using a Covaris S220 (Covaris, United States). Library preparations were performed using a NEBNext Ultra II DNA kit (New England Biolabs, United States) following the manufacturers protocol. A library validation was performed using an Agilent 2100 Bioanalyzer with a DNA High Sensitivity chip (Agilent, USA) and quantified via qPCR (AriaMx QPCR (Agilent, USA).