title: GSM6423658: polyA RNA from Myc overexpression mouse kidneys, replicate 1 [ZYY_003_HET_AD005]; Mus musculus; RNA-Seq
library_construction_protocol: For RNA extraction, the entire right kidney was removed and kept in Qiazol until used, and then lysed using a tissue homogenizer. Total RNA extraction was performed using the RNeasy Mini Kit (74104, Qiagen).  For RNA purity, DNase digestion was performed using the RNase Free DNase Set (79254, Qiagen) according to the manufacturer's instructions. RNA was eluted in 30 μl RNase-free H2O. RNA-sequencing libraries were constructed using the TruSeq RNA Sample Prep Kit v2 (Illumina)