title: IlluminaMiseq  sequence data: soil bacteriome
design: About 0.25 g of each rhizosphere soil sample was used to extract DNA commercial kit and PCR targeting the V3V4 hyper-variable regions of the 16S rRNA gene. using the universal primer pair 341F forward primer and uniquely barcoded 785R reverse primer for each sample. Resulting amplicons were gel purified, end-repaired and Illumina TruSeq adapters were ligated to each amplicon. Then samples were individually indexed, and another bead-based purification step was performed. Following quantification and equimolar pooling, amplicons were then sequenced on Illumina's MiSeq platform, using MiSeq reagent kit v3 (600 cycles).