title: GSM7829725: AAV-GFP_1, ATAC; Mus musculus; ATAC-seq
library_construction_protocol: Nuclei from each sample was isolated separately with 1 ml homogenization buffer in a 2-ml dounce homogenizer. Use Pestle B to dounce it 20 times to ensure isolation of nuclei from cells with minimal damage. Transfer the lysis solution and 3 ml homogenization buffer to individual NalgeneTM centrifuge tubes containing 8 ml Sucrose Cushion buffer. Centrifuge for 1 h at 20,000 rpm, 4°C. Carefully remove the middle layer, which contains a lot of white particles. Discard all the supernatants. Resuspend nuclei in 40 µl Pre-Tagmentation Master Mix for tagmentation reaction. ATAC libraries were prepared using ATAC-Seq Kit (Active Motif # 53150).