title: GSM8121281: FC1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cell lysate using an RNAspin mini RNA isolation kit (25-0500-72, Cytiva, Marlborough, MA, United States) RNA libraries were developed using the NEBNext Ultra II Directional RNA-Seq library prep kit (E7760S, New England Biolabs, Inc., Ipswich, MA, United States). The resulting libraries were quantitated using the KapaBiosystems qPCR quantitation kit (MilleporeSigma, Burlington, MA, United States)