title: scRNA-seq of mouse pancreatic islets from non-transgenic and pericyte MyD88-mutated mice
design: scRNA-seq of mouse pancreatic islets from non-transgenic and pericyte MyD88-mutated mice
library_construction_protocol: Isolated islets from wild-type mice and Nkx3.2-Cre;MyD88 f/f mice at 15 weeks of age  For islet isolation, 3 ml of cold collagenase P solution (0.8 mg/ml, Roche) was injected into the pancreas of euthanized mice using a 30G needle. The pancreas was then removed and incubated with collagenase P at 37ºC for 10-11 minutes, followed by termination with cold RPMI medium 1640 (Gibco) and subsequent centrifugation. The resulting pancreatic pellet was washed, strained, and subjected to density gradient separation using Histopaque-1119 (Sigma-Aldrich) and RPMI medium, followed by another round of centrifugation. Islets were isolated by dispersing double-hand-picked islets in Accutase (Sigma-Aldrich) for 5 minutes. The isolation of immune cells was performed through Magnetic Activated Cell Sorting (MACS, Miltenyi Biotec).   None Chromium Next GEM single Cell 3' v3.1