title: Rat7_LDG_cDNA
library_construction_protocol: Isolated brain tissue (dentate gyrus) was homogenized in 1 mL of Trizol. RNA was then extracted from supernatant using standard manufacturer's protocol. RNA was quality assessed using Nanodrop 2000 Spectrophotometer, then subjected to further purification by precipitation with 3 volumes of KAPA Pure Beads according to manufacturer's instructions. 200 ng of total RNA was used to prepare nanopore cDNA sequencing libraries with the cDNA-PCR Sequencing Kit SQK-PCS111 (ONT) according to the manufacturer's instructions. Sequencing was carried out using FLO-MIN106 R9.4.1 RevD flow cells on a MinION device controlled by MinKNOW software (ONT).