title: ATAC-seq biological replicate 2 in human parathyroid
design: ATAC-seq was performed on human parathyroid adenoma tissue following a published protocol with minor modifications. Nuclei isolation steps were conducted on ice. Frozen tissue (10 mg) was homogenized in 200 µL cold 1x homogenization buffer using a Dounce homogenizer. The homogenate was filtered and subjected to additional douncing. After centrifugation, the pellet was resuspended in homogenization buffer and layered over iodixanol gradients. Following centrifugation, the pellet was resuspended in transposition reaction mix from the Nextera XT kit and subjected to transposition at 37°C for 30 minutes. The reaction was purified, and transposed DNA fragments were amplified using high-fidelity PCR. Libraries were prepared and sequenced using DNB-seq paired-end 100bp at BGI Americas.