title: ChIP-seq input of wild type P. falciparum NF54 parasites
design: Infected RBCs were collected at 16 h.p.i., lysed using 0.1% saponin (Sigma) and cross-linked in 1% methanol-free formaldehyde (Polysciences Inc). Chromaitin was sheared using the Covaris ME220 ultra sonicator. Prior to immunoprecipitation, a small aliquot was made to use as an input control. ChIP-seq libraries were prepared using the KAPA LTP library preparation kit (KAPA Biosystems), then amplified using the KAPA HiFi HotStart ready mix (KAPA Biosystems) and purified with 1X KAPA Pure beads (KAPA Biosystems).