title: 16S rRNA from mouse feces
design: DNA was extracted from mouse feces using Qiagens QIAamp Powerfecal Pro kit and V4-V5 region from 16S rRNA gene was amplified using primers designed with Golay barcodes. Purified amplicons were pooled at equimolar concentration and libraries were prepared using Qiagens QIAseq one step amplicon library kit.  QCed libraries were sequenced on Illuminas MiSeq platform at 2x250bp read lenghth.