title: A One Month High Fat Diet Disrupts the Gut Microbiome and Integrity of the Colon Inducing Adiposity and Behavioral Despair in Male Sprague Dawley Rats
design: Colonic samples from sprague dawley were amplify using V1-V3 region from bacterial 16srDNA gene. Amplicon were verified by electrophoresis and then 2ng were prepared using nextera XT DNA Kit following by run in a MiSeq instrument with a 500 cycles. Miseq data were analyzed using QIIME sofware.