title: 0700450231_MSP_060_3pp
design: A small volume (50ul) of blood was collected during each trimester, at delivery, and during the first and third months of post partum for the blood transcriptome profiling. Blood samples were mixed well with tempus solution (100ul) and stored at -20C until further procesing. Total RNA was extracted from whole blood lysate using Tempus Spin RNA extraction kit. RNA libraries for RNA-seq were prepared using TruSeq mRNA-Seq Library Prep Kit following manufacturer's protocols. Processing of RNA-Seq data was done using bcbio rnaseq pipeline, bcbio version 1.2.3. Alignment was done using STAR version 2.6.1d and reads were mapped to the hg38 genome. After alignment, Samtools 1.3 was used to collect metrics on bam which will be used further to generate a multiqc report.