title: WGS of NAm strains of Histoplasma
design: Isolates were grown on brain heart infusion (BHI) agar at 25C not more than 10 days. Genomic DNA was extracted by using the DNeasy Blood and Tissue kit (Qiagen, Gaithersburg, MD) according to the manufacturers recommendations and verified using 0.8% gel electrophoresis and quantification and purity was measured on a NanoDrop 2000c spectrophotometer. All procedures were conducted in BSL3 laboratory due to high fungal infectivity of Histoplasmas mycelial phase. All paired-end sequencing libraries were prepped using the Kapa Biosystems kit (Kapa Biosystems, Woburn, MA) and about 1ug of DNA was used as input material. DNA sequencing was performed on a Illumina HiSeq 2500 (100 bp, high output v3 or v4 chemistries) or in a Illumina NextSeq 550 equipment (150bp, high output v2).