title: RNA sequencing of adipose tissue-derived mesenchymal stem cells: control rep2
design: To perform RNA-Seq analysis, total RNA was extracted from each cultured adipose tissue-derived mesenchymal stem cells. Sequencing library was generated using Truseq Stranded mRNA LT Sample Prep Kit. Around 300 bp fragments were isolated using gel electrophoresis, amplified by PCR and the quality of sequencing library was assessed by BioAnalyzer. The library was sequenced on the Illumina HiSeq 2500 in the paired-end sequencing mode (2x101 bp reads).