title: TCR-Seq of galllus gallus: female spleen gamma chains
design: Total RNA was isolated from splenic tissue using silica minicolumns and on-column incubation with DNase I. TCR-specific 5'RACE with C-specific reverse primers and a UMI (UNNNNUNNNNUNNNNU) - containing TSO was conducted. Two rounds of PCRs were used to amplify the cDNA followed by purification of TCR amplicons from an agarose gel. Libraries were then generated by Eurofins Genomics Germany GmbH (2nd PCR Amplicon option).