title: RNA-sequencing on EN purified from GW20 cerebral cortex: Sample S19-D5
design: Radial glia (RG),  Intermediate progenitor cells (IPC) and Excitatory neurons (EN) were purified from human cortical brain tissue at gestational weeks (GW) 20  using flourescence activated nuclei sorting (FANS). RG, IPC and EN sorted nuclei were subjected to RNA-sequencing using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturer instructions.  Libraries were sequenced using 150-bp paired-end sequencing on Illumina NovaSeq 6000.