title: 16SrRNA-Seq of bacteria: Female vagina of childbearing age
design: Genomic DNA was extracted from a female vaginal swab. A library was constructed using PCR primers 341F (50-ACTCCTACGGGAGGCAGCAG-30) and 806R (50-GGACTACHVGGGTWTCTAAT-30) to amplify the 16 S rRNA gene (V3-V4 region), and PCR amplification was performed. The libraries were identified using Qubit and Q-PCR, and sequenced using the Illumina MiSeq platform.