title: GSM8334593: eNPCs, Hq, proliferating, rep4; Mus musculus; RNA-Seq
library_construction_protocol: At the indicated time points, cells were scraped, collected and pelleted in PBS. Cell pellets of approximately 500,000 cells were then lysed in Trizol, snap-frozen and stored at -80 °C until use RNA was quantified via HS RNAassay on a Tapestation4200 instrument (Agilent). RNA was extracted from wt and Hq mutant eNPCs collected at the indicated time points. Approximately 100 ng of extracted RNA were used as input for RNA‐Seq library preparation according to the TruSeq Stranded mRNA kit (Illumina). Next generation sequencing libraries were quantified via Qubit HS dsDNA assay (Invitrogen) and library size distribution was determined using a HS D1000 assay on a Tapestation4200 instrument (Agilent). Libraries were equimolarly pooled, clustered at 1200 pM concentration, and sequenced SR 75 cycles on a NovaSeq6000 instrument (Illumina) using an S1 v1.5 chemistry.