title: methylation-seq of healthy male
design: After comprehensive DNA library analysis, the fragments were sequenced using the Illumina NovaSeq 6000 system (Illumina, Inc., San Diego, CA, USA), with the resulting raw data being stored in the FASTQ format. Base calling was performed using Illumina Casava 1.8, and data quality was assessed and refined using FastQC and Trimmomatic. In brief, we first used FastQC v0.11.7 to perform basic statistics on the raw data.