title: USP22 supports the aggressive behavior of basal-like breast cancer by stimulating cellular respiration
design: USP22 supports the aggressive behavior of basal-like breast cancer by stimulating cellular respiration
library_construction_protocol: HCC1806 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Dharmafect 1 (Horizon Discovery) according to the manufacturer's instructions. RNA was extracted using EXTRAzol® Lysis Reagent (BLIRT) according to the manufacturer's protocol. Libraries were prepared using TruSeq RNA Library Prep Kit v2 (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100.