title: DAPseq using MYB137 in Eucalyptus
design: Genomic DNA was extracted using the NucleoSpin Plant Kit (Machery-Nagel). After sonication and end-repair, truncated illumina adapters were ligated to E. grandis sheared gDNA and used for DAP-seq analysis using EgrMYB1 and EgrMYB2 (E3) or EgrMYB137 protein fused to HALO tag. DNA affinity purification, library amplification (introduction of full indexes) and library pooling were all carried out as previously described (Bartlett et al. 2017) with minor modifications.