title: single-nuclei RNA-Seq of human normal adult adrenal glands
design: Single-nuclei RNAseq library generated using the inDropTM technique from 1CellBio. Nuclei were extracted from snap-frozen samples taken from tumor sections during tumor removal surgery, using previously published procedure (Krishnaswami et al. 2016). The steps of salinization, reverse transcription (RT), and library preparation were executed following the inDropTM system from 1CellBio (Baron et al. 2016). This was done on chips containing hydrogel beads. Library preparation was conducted as per the CEL-Seq2 protocol(Hashimshony et al. 2016), which involved digesting the RT product with ExoI and HinFI enzymes, followed by purification using AMpure XP beads (Beckman Coulter, USA). The generated cDNA was measured using qPCR (Roche Light Cycler 480 Instrument II, Switzerland) and combined for sequencing. Sequencing was done using an Illumina HiSeq 2000 instrument, producing reads of 60 bases for read 1, 6 for the Illumina index, and 50 for read 2.