title: GSM7830298: Infected AT2 -3; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were harvested from control and 4 day infected mice, single cells suspension was prepared by mehcanical and enzymatic digetsion.  CD45+ and CD31+ cells were depleted using biotinylated antibodies followed by surface staining with antibody mix to identify At2 and transitional At2 cells. At2 cells were identified as CD45-CD31-Epcam+CD104-MHCII hiCD51- and transitional At2 cells  CD45-CD31-Epcam+CD104-MHCII hiCD51+. Dead cells were excluded by Dapi staining. Libraries were prepared using Illumina stranded total RNA with Ribo-zero Plus kit accordine to manufacture instructions