title: DNA seq of blood: pooled control samples
design: DNA was isolated from the whole bloodusing the FlexiGene isolation kit (Qiagen, Cat#51206). Participants' DNA was pooled according to their values of HbA1c. The DNA input amount was 3 g. Pooled samples were sonicated with average lenght of 3000 bp. Libreries were prepared with a Ligation sequencing kit (ONT, product code SQK-LSK109) following the manufactures protocol. Six labries were prepared. Final libraries were sequenced seperatly on a Promethion flow cell (R9.4.1, ONT, product code FLO-PRO002). After sequencing guppy basecaller (v6.3.8) together with high accuracy Remora model (dna_r9.4.1_450bps_modbases_5hmc_5mc_cg_hac) was used for alignment on hg38.