title: mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups
design: mRNA-Seq of Danio rerio exposed to different concentrations of the fungicide metalaxyl against untreated control groups
library_construction_protocol: : at the end of exposure, 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep-24 homogeniser (MP Biomedicals, Irvine, USA) at 5 m/s speed for 45 seconds. Zebrafish embryos were exposed to metalaxyl for 96 hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to each of two sub-lethal concentrations of metalaxyl (high exposure and low exposure concentrations) in addition to untreated control groups. RNA libraries were prepared from RNA extracts with RNA integrity number (RIN) values > 8 using poly(A) RNA purification and the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, USA). RNA libraries were prepared from RNA extracts with RNA integrity number (RIN) values > 8 using poly(A) RNA purification and the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, USA).