title: GSM7964505: Custom Cell Hashing Library; Mus musculus; RNA-Seq
library_construction_protocol: Lungs freshly dissected from euthanized mice at 48 hours after LPS treatment were dissociated into single cell suspension. The cell samples from each group were stained with TotalSeq CD45 antibody conjugated with unique oligo barcodes purchased from BioLegend. All hashtagged samples were pooled together for GEM partitioning and library construction using 10x genomics single cell 5' kit. The gene expression and cell hashing libraries were constructed according to the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping (10× Genomics, CG000330 Rev A).