title: GSM6658918: HeLa RCA long reads; Homo sapiens; OTHER
library_construction_protocol: DNA isolation by column separation（Plasmid Mini AX; A&A Biotechnology）, removal of remaining linear DNA by exonuclease（Plasmid-Safe ATP-dependent DNase; Lucigen, PacI enzyme; NEB）, and rolling-circle amplification by random hexamer. For short-reads, DNA were fragmented by sonication. For long-reads, DNA fragments were selected by the BluePippin system. Short-reads and long-reads libraries were constructed and sequenced following MGI's and Nanopore standard protocol, respectively.