title: GSM6755876: Ctrl, Heart failure, replicate3, RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed by CO2 inhalation, and hearts were immediately cannulated and perfused with cold PBS. After the heart was harvested, the atria and valves were removed, and  RNA was extracted using Trizol reagent. 500 ng total RNA is used for rRNA-depletion (NEB, E6310), followed by directional library synthesis (NEB, E7420)