title: Amplicon-Seq of Wastewater Influent: Linear Pipe Collection site
design: Wastewater influent grab sample collected from a linear pipe collection site in York, Ontario, Canada. Microbes were isolated using Ceres Nanotrap Microbiome A isolation beads, RNA was extracted and PCR-amplifed with primers specific to the Tomato Brown Roguse Fruit Virus genome sequence. PCR amplicons were prepared for Illumina sequencing using the Illumina DNA Library prep kit.