title: RNA-Seq of Soybean
design: The soybean seedlings were sampled to conduct the transcriptomic analysis in LC-Bio company (Hangzhou, China). Total RNA was isolated from the samples using the Spectrum Plant Total RNA Kit (SigmaAldrich, St. Louis, MO, United States, STRN10-1KT) and then mixed with fragmentation buffer, which enriched and fragmented mRNA to form first-strand cDNA by further using random hexamer primers. T4 polynucleotide kinase, T4 DNA polymerase, and DNA polymerase I Klenow fragment were then used to repair the ends of the double-stranded cDNAs in preparation for ligating the fragments to attachments with T4 DNA ligase. Following PCR amplification to select and enrich the available fragments for library construction, the fragments were sequenced using an Illumina NovaSeqTM 6000 after qualification and quantification with an Agilent 2100 Bioanalyzer and an ABI StepOnePlus Real-Time PCR System.