title: Illumina HiSeq 2000 paired end sequencing; Nfkb2 deficiency and its impact on the murine small intestinal mucosa
design: Nfkb2 deficiency and its impact on the murine small intestinal mucosa
library_construction_protocol: C57BL/6 or transgenic male mice, between 8-10 weeks old, were culled by CO2 administration. The proximal small intestine was dissected and rinsed with phosphate buffer saline (PBS) and mucosal scrapes were used for isolation of total RNA using the RNeasy Mini Kit (QIAGEN).  isolation of total RNA using the RNeasy Mini Kit (QIAGEN) Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina)