title: RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc
design: RNA-seq of Mycobacterium smegmatis MC2-155 with or without inducible overexpression of Mtb RpfB UTR with Atc
library_construction_protocol: Samples were grown to exponential phase, centrifuged to pellet and resuspended in FastRNA Pro  Samples were grown in 7H9 with ADC (1:10) and tyloxapol (0.05%, v/v), shaking at 200 rpm, 37C until OD600nm = 0.6-0.9 Cells were lysed in a sample homoginizer (6m/s, 40s), and total RNA extracted as per the instructions for the FastRNA Pro kit. IDT xGen RNA Library Preparation Kit