title: GSM8370234: P_2; Panax japonicus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 15 samples of Panax japonicus pericarp (5 different fruit colour traits, 3 biological replicates) using Tiangen Kit, RNAprep Pure. The integrity and quality of RNA was assessed by agarose gel (Agarose horizontal electrophoresis machine model DYCP-32C) and Agilent Agilent 2100 Bioanalyzer. The first strand of cDNA was synthesised in M-MuLV reverse transcriptase system using fragmented mRNA as template and random oligonucleotides as primers, and the second strand of cDNA was synthesised using dNTPs as raw material. The double-stranded cDNA was purified and subjected to PCR amplification to finally obtain the library. Qubit2.0 Fluorometer was used for preliminary quantification, and the library was diluted to 1.5 ng/ul. Agilent2100 bioanalyzer was used to insertsize the library to ensure the quality of the library.