title: GSM8438338: MSC, Ground control, O1; Homo sapiens; RNA-Seq
library_construction_protocol: MSCs were trypsinized and subsequently preserved in RNAprotect solution followed by isolation utilizing the miRNeasy Mini kit. cDNA libraries were prepared using up to 200 ng of total RNA according to the manufacturer's instructions for the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA). Libraries were sequenced at an average of 35M read pairs per sample with Illumina's standard protocol for the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit. The flow cell was sequenced as 100 X 2 paired end reads on an Illumina HiSeq 4000 using the HiSeq 3000/4000 sequencing kit and HD 3.3.52 collection software. Base-calling was performed using Illumina's RTA version 2.7.3.