title: GSM8368445: muscle, WT-Con1-3; Mus musculus; RNA-Seq
library_construction_protocol: The tissues were immediately snap-frozen in liquid nitrogen and stored at -80°C. Total RNA was extracted using a suitable RNA extraction kit and assessed for quality and quantity. High-quality RNA (RIN > 7) was used for library preparation, including mRNA enrichment or rRNA depletion, cDNA synthesis, and adaptor ligation. The libraries were then sequenced on an Illumina platform with paired-end sequencing. Sequencing data underwent quality control and alignment to the reference genome for further analysis. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.