title: GSM8162955: MOCK, WVC1, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were removed at days 1, 3, and 6 days post-infection by adding 1 mL of accutase solution (Sigma A6964-100mL) into the basolateral side of the transwell. Cells were incubated at 37 degrees Celsius for 30 minutes until cells were detached. Cells were centrifuged at 4 degrees celsius at 350 rpm for 5 minutes, were resuspended, and processed for scRNA-Seq on a 10× Genomics Chromium Controller using 5′ Next GEM v1.1 reagents (10× Genomics, Inc) according to the manufacturer's protocol.