title: Ethanol pool gRNA library in Yeast, 2nd passage, Sequencing replicate 3
design: The gRNA libraries targeting ethanol related pathway were introduced into yeast cells, which were cultivated in a chemically defined medium with 20 g/L ethanol as the major carbon source. The cells were grown in flasks under aerobic conditions at 30 C, and three biological replicates were performed in parallel. Three passages of the cell culture were conducted to exert selection pressure on the yeast cells. To investigate gRNA content changes between different passaging stages, plasmids were extracted from yeast cells and nanopore sequencing was employed to analyze gRNA enrichment or depletion through this process. This is the data for the second passage, one of the sequencing replicates.