title: GSM8208287: hybrids D. Melanogaster x D.simulans, ovaries, small RNA rep1; Drosophila melanogaster x Drosophila simulans; ncRNA-Seq
library_construction_protocol: For small RNA libraries preparation RNA was extracted from 0-3 days old gonads of D. melanogaster Hmr2, D. simulans w501 and interspecific hybrids. Small RNA fraction (19-29 nt) was purified by 15% polyacrylamide gel from 20-30 μg total RNA. Small RNA libraries were prepared using NEBNext Multiplex Small RNA Sample Prep Set for Illumina (#E7300S, NEB) and sequenced on NovaSeq 6000 in 100 bp single-end mode yielding 25-40 million single-end reads per library.