title: RNA sequencing of Ensete ventricosum: pseudostem
design: Total RNA extraction was done using the QIAGEN RNeasy Plus Kit following manufacturer protocols. Total RNA was quantified using Qubit RNA Assay and TapeStation 4200. Prior to library preparation, DNase treatment was performed followed by AMPure bead clean up and QIAGEN FastSelect HMR rRNA depletion. Library preparation was done with the NEBNext Ultra II RNA Library Prep Kit following manufacturer protocols.