title: Detection of MEOX2 De Novo Motif Sites And Distal Intergenic Sequences By ChIP-Seq
design: The DNA size profile was evaluated in a bioanalyzer 2100, for the libraries assemble the kit Truseq Chip de Illumina was used, using a total of 10 ng. Cutted DNA fragments were performed from 300 to 500 bp, the distribution of the amplified libraries were evaluated by TapeStation 4200, the final loading concentration was of 1.5 pM and a 30% of control PhiX was included. For the sequenciation we used a kit Nextseq 500/550 High Output (150-cycles) Flow Cell.