title: GSM8438822: T1 young D1; Mus musculus; RNA-Seq
library_construction_protocol: Primary ovarian somatic cells were lifted from 2D monolayer culture or dissociated from organoids with 0.05% (monolayer) or 0.25% (organoids) trypsin. Trypsin was quenched with RPMI-1640 containing 10% FBS and 1% Penicillin-Streptomycin and the cell suspension was strained through a 40 µm cell strainer. Cells were pelleted by centrifugation at 500 x g for 5 min at room temperature, washed with DPBS containing 1% BSA, and resuspended in 100 µL DPBS containing 1% BSA. Cell number and viability were analyzed using a Nexcelom Cellometer Auto2000 with the AOPI fluorescent staining method. 10X Genomics NEXT GEM Kit V2, 10X genomics Library Construction kit