title: Investigating impacts of mycothiazole and its synthetic derivative on mitochondrial function and aging in C. elegans
design: Investigating impacts of mycothiazole and its synthetic derivative on mitochondrial function and aging in C. elegans
library_construction_protocol: RNA collection was performed by removing growth medium and washing 1x with PBS, then adding Trizol directly to cells, cell + trizol was frozen in -80C until extraction was performed.   Cells were grown in high glucose DMEM + 10%FBS + 1x pen/strep at 5% CO2 37°C. Drugs were diluted in growth medium, which was applied directly to cells for 1 hrs prior to sample collection.  After the final thaw, chloroform was added at a 1:5 chlorform/trizol ratio and aqueous separation of RNA was performed via centrifugation in a heavy gel phase-lock tube (VWR, 10847-802). The aqueous phase was mixed 1:1 with isopropanol then applied to a Qiagen RNeasy Mini Kit (74106) and RNA purification was performed as per manufacturer's directions.  Library construction was performed by Novogene using their standard pipeline.