title: E. coli DH10B-GEM host with BBa_K598014 BioBrick on pSB1C3 backbone plasmid
design: Plasmid DNA was purified using a QIAprep Spin Miniprep Kit (QIAGEN) or a PureLink Quick Plasmid Miniprep Kit (Invitrogen). For Illumina sequencing, up to 10 ng of plasmid DNA was used as input for sequencing library preparation using the 2S Turbo DNA Library kit (Swift Biosciences) with 20% reaction volumes. Custom adapters containing dual 6-bp indexes were incorporated during the final PCR step.