title: GSM7783755: B. subtilis 9407 derived strain, inactive PNPase, D493A group 2; Bacillus subtilis; RIP-Seq
library_construction_protocol: Target mRNAs were extracted using TRIzol reagent (Invitrogen), followed by determination of RNA concentration and quality analysis conducted by Seqhealth Technology Co., Ltd (Wuhan, China). The stranded RNA sequencing library was constructed using KC-DigitalTM Stranded mRNA Library Prep Kit for Illumina® (Seqhealth) following the manufacturer's instruction. The library products corresponding to 200-500 bps were enriched, quantified, and finally sequenced using the PE150 model on a Novaseq 6000 sequencer (Illumina).