title: GSM6756918: Saline-expt2-rep2; Mus musculus; RNA-Seq
library_construction_protocol: At day 21 after PPE or saline treatment, lung lobes were inflated and submerged with dispase  at room temperature for 45min. The digested lung lobes were further homogenized in DMEM/F12 with PenStrep and DNAseI. The single cell suspension was sequentially filtered through 70µm and 40µm cell strainers on top of 50ml tubes, followed by centrifuge at 300g for 10min at 15°C. Epithelial cells were enriched with anti-mouse CD326  and MACS LS column. Cells were stained with APC anti-mouse Epcam and DAPI to FACS sort for Epcam+/DAPI-/tdTomato+ cells for single cell capture with 10x Chromium system. Single cell libraries were generated using the 10x genomics chromium 3' end library prepartion kit.