title: fungal ITS1 region of soil sample : 181F
design: We amplified fungal ITS1 region, recognized as DNA barcode for fungal identification, with the forward ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA) and reverse ITS2 (5'-GCTGCGTTCTTCATCGATGC) primers (Gardes and Bruns, 1993; White et al., 1990). We performed the amplification on 1 L of template DNA in a volume of 25 L using Taq PCR Master Mix Kit (QIAGEN). The program was as follows: 3 min at 94C, 35 cycles of 1 min at 95C, 1 min 52C and 1 min at 72C, and a final step 10 min at 72C.