title: microsporidian diversity in lake Aydat through metabarcoding
design: DNA was extracted using phenol-chloroform. Amplification of the V1V3 region of the microsporidian gene coding for the 18S rRNA was performed using the universal microsporidian V1F and 530R set. The amplicons were purified and concentrated using the QIAquick Gel Extraction kit. See Chauvet et al. 2022 for the detailed protocol.