title: GSM8073080: EV, Control male, CTRL19; Homo sapiens; miRNA-Seq
library_construction_protocol: QIAzol lysis reagent was added at 5X to each sample, which were then incubated at room temperature for 5 min. 1X of chloroform was added, and the samples were incubated at room temperature for 3 min. They were centrifuged at 12000g at 4 °C for 15 min, and the aqueous phase was collected. 1.5 volumes of 100 % ethanol were added and mixed gently. The mixture was then added to QIAGEN MinElute columns, and the MiRNeasy Micro Kit was used according to the manufacturer's protocol (QIAGEN). Libraries were constructed follwing the Galas Lab - 4N library prep - Version 1.0 protocol