title: RNAseq of ccRCC cells with RARRES2, CMKLR1, or GPR1 knocked out
design: RNAseq of ccRCC cells with RARRES2, CMKLR1, or GPR1 knocked out
library_construction_protocol: subconfluent cells harvested with Trizol 769-P cells infected with lentivirus containing CAS9 and specific gRNA; puromycin selection; then harvested. phenol/chloroform extraction, isopropanol precipitation poly A purfication, cDNA preparation