title: RNA-Seq of Drosophila melanogaster: adult CS CNS
design: The cleaved RNA fragments are copied into first strand cDNA using SuperScript II reverse transcriptase and random primers. This is followed by second strand cDNA synthesis using DNA Polymerase I and RNase H. The cDNA fragments then go through an end repair process, the addition of a single A base, and then ligation of the Illumina multiplexing adapters. The products are then purified and enriched with PCR to create the final cDNA sequencing library.