title: GSM8033465: Astrocytes Type 1 transplanted with BTIC mitochondria; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cells using Invitrogen (Waltham, MA, USA) TRIzol and purified using Invitrogen (Waltham, MA) Turbo DNase following the manufacturer's protocols. RNA was cleaned and concentrated using Zymo (Irvine, CA, USA) RNA Clean and Concentrator MagBead following the manufacturer's protocols. RNA-seq libraries were prepared using the Lexogen (Greenland, NH, USA) CORALL Total RNA-seq kit with UDIs (PN M11696 and PN M10096-2-0101) and the Lexogen (Green-land, NH) RiboCop rRNA Depletion kit V2 (PN K14496). Illumina (San Diego, CA, USA) NovaSeq SP 2 × 150 was used to sequence the RNA-seq libraries following the manufacturer's protocol.