title: GSM6500404: Treg-depleted; Mus musculus; RNA-Seq
library_construction_protocol: Lymph nodes were cut into small fragments and digested as described in the manuscript. CD4 T cells were enriched on autoMACS Pro Separator (Miltenyi Biotec). CD4+CD45.1+ SMARTA cells were FACSorted on a Moflo Astrios (Beckman Coulter) or a BD FACSaria Fusion cell sorter. After sorting and purity check on flow cytometer, SMARTA cells were then resuspend in 10X Genomics Chromium single cell RNA master mix and loaded onto a 10x chromium chip. cDNA Library was generated using the Chromium Single Cell 3' Reagents Kits v2 User Guide:CG00052 Rev B, and then sequenced on the Illumina HiSeq 2500 platform to achieve an average of 40,000 reads per cell. Sequencing was performed at the Princess Margaret Genomics Center.