title: GSM7112082: Donor 1, Time 4; Homo sapiens; RNA-Seq
library_construction_protocol: Snap frozen constructs were pulverised in a Cryo-Cup grinder on dry ice and RNA extracted using Trizol and cleaned up with a Direct-Zol RNA miniprep kit and RNA clean and concentrator (Zymo Research, Cambridge, UK) according to the manufacturer's instructions. RNA libraries were constructed using the NEBNext Poly(A) mRNA magnetic isolation module (New England Biosystems, Ipswich, UK) and then the NEBNext ultra-directional RNA library prep kit was used to create the final library. The sample was sequenced using 41bp paired end configuration with an Illumina NextSeq 500 sequencing device.