title: GSM6427509: MDA-MB-231 + pLV-KRAB + all 4 gRNAs, biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed twices before RNA was harvested using QIZsol Lysis reagent (QIAGEN). Approximately 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared by Australian Genome Research Facility, where libraries were preprared sequenced for 50bp single-end (SE) reads on the Illumina HiSeq 2500 system