title: GSM7831028: U251, PRSS35, 1Gy, 6h; Homo sapiens; OTHER
library_construction_protocol: The 4C (chromosome conformation capture) technique was carried out as previously described in Schwartz M et. al., 2015. The cross-linked chromatin was subjected to overnight digestion with an excess amount of HindIII enzyme (New England Biolabs). Subsequently, the DNA ends were ligated under diluted conditions that promote the formation of junctions between cross-linked DNA fragments. The ligated junctions were then circularized through digestion with DpnII (Thermo Scientific). Chromosomal interactions with the target regions, known as baits, were amplified using inverse PCR primers (Supplementary Table S3) and Platinum Taq DNA Polymerase (LifeTechnologies). The Illumina Hi-seq 2000 platform was used to sequence 4C-seq libraries.