title: GSM6865842: KO_GMP_RNA_A; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted from BM. Total RNA samples were isolated using RNeasy Mini Kit (Qiagen). Sample QC, library preparations, and sequencing reactions were conducted at GENEWIZ, LLC./Azenta US, Inc. ERCC RNA Spike-In Mix  was added to normalized cell number prior to library preparation. The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions. The  libraries were validated on the Agilent TapeStation, and quantified by using Qubit 2.0 Fluorometer. Libraries were sequenced on the NovaSeq 6000 using a 2x150bp Paired End (PE) configuration