title: GSM7078348: Klrg1neg_MTRlow:Klrg1neg_MTRlow_3; Mus musculus; RNA-Seq
library_construction_protocol: Bulk MAIT cells were sorted by flow cytometry into 500 μL of Trizol LS (Thermo Fisher). RNA extraction, reverse transcription, cDNA amplification, fragmentation, additional PCR amplification, final library purification, and quality control were performed as previously described in Riffelmacher et al, Mucosal Immunology, 2018. Sequencing was performed using the HiSeq2500 sequencer (Illumina). final library purification, and quality control were performed as previously described in Riffelmacher et al, Mucosal Immunology, 2018