title: eukaryotic diversity in lake Aydat through metabarcoding
design: DNA was extracted using phenol-chloroform. Amplification of the eukaryotic V4 region was performed using the universal primer 515F and the eukaryotic primer 951R. The amplicons were purified and concentrated using the QIAquick Gel Extraction kit. See Chauvet et al. 2022 for the detailed protocol.