title: GSM8016937: iPSC-EC, DSCAM -/-/-, rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: cells were homogenized in Trizol Reagent (Life Technologies, Inc., Grand Island, NY) with TissueLyzer II (QIAGEN, Inc., Valencia, CA) and RNA was isolated by conventional methods. RNA went through two rounds of mRNA purification (polyA-selection) using Dynabeads mRNA DIRECT Kit (Invitrogen, Carlsbad, CA). Double-stranded cDNA was generated using the Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). The cDNA products were used to construct libraries with the Nextera XT DNA Sample Preparation Kit (Illumina, Inc., San Diego, CA). Libraries were paired-end sequenced with a read length of 75 base pairs (75PE) on the Illumina NoaSeq 600.