title: GSM7111569: BDNF_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was separated from protein and DNA by mixing 500μL sample in Trizol with 100μL chloroform before ultracentrifugation (15min, 16000g, 4°). The upperphase was mixed with a half volume of 100° isopropanol, transferred to a purification column, and the RNA purified using the kit PureLink™ RNA Micro Scale (Thermofisher #12183016) following manufacturer's instructions. RNA from all samples were extracted using the the kit PureLink™ RNA Micro Scale (Thermofisher #12183016), quality controlled, before mRNA library preparation mRNA KAPA mRNA HyperPrep Kit (KapaBiosystems #KR1352). All barcoded samples were then sequenced with a Nextseq500 (Illumina) with 75-cycle single-end read.