title: GSM8208873: Neuroinvasion; Mus musculus; RNA-Seq
library_construction_protocol: Isolated PBMCs from the mice were transported in sterile culture dish with 10 ml Dulbecco's Phosphate-Buffered Saline (DPBS, Thermo Fisher Scientific) on ice to remove the residual tissue storage solution, then minced on ice. We used dissociation enzyme 0.25% Trypsin (Thermo Fisher Scientific) and 10 ug/mL DNase I (Sigma) dissolved in PBS with 5% Fetal Bovine Serum (FBS, GIBCO) to digest the tissues. 10× beads that were then subject to second-strand cDNA synthesis, adaptor ligation, and universal amplification. Sequencing libraries were prepared using randomly interrupted whole-transcriptome amplification products to enrich the 3' end of the transcripts linked with the cell barcode and UMI. All the remaining procedures including the library construction were performed according to the standard manufacturer's protocol (Chromium Single Cell 3ʹ v3).