title: PromethION sequencing; Oxford Nanopore long reads from Cenchrus americanus
library_construction_protocol: High molecular weight DNA extraction was performed using a previous published protocol (Mariac et al. 2019). Briefly, the isolation of plant nuclei is performed from 1 gram of young fresh leaves previously ground in liquid nitrogen. The isolated nuclei are then lysed (MATAB) and the DNA purified with chloroform/isoamyl alcohol (24:1) and then precipitated with isopropanol. All transfer steps were performed with a pipette tip cut at the extremity and homogenization steps were performed by slow inversion to limit mechanical shearing of the DNA molecules. DNAs were quantified by fluorometry (Qubit) and qualitatively assessed using pulsed field electrophoresis to ensure that fragment sizes ranged from 40-150Â kb. Oxford Nanopore DNA library preparation (SQKLSK109-PromethION, Genomic DNA ligation protocol) and sequencing were performed by Novogen Co., LTD.