title: GSM7245918: Jag1ff/VavCre+ mac 1; Mus musculus; RNA-Seq
library_construction_protocol: 1-3x10^3 fetal liver-derived CD169+ macrophages from Jag1ff/VavCre- and Jag1ff/VavCre+ E14.5 embryos were sorted into RNA lysis buffer (Invitrogen) and stored at -80ºC until RNA extraction. Total RNA was extracted using PicoPure™ RNA Isolation Kit (ThemoFisher Scientific, USA) with inclusion of DNase treatment step. RNA sequencing libraries for Illumina sequencing were prepared in one batch using  SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) along with negative and positive controls. We used 1 nanogram of total input RNA and eleven PCR cycles during cDNA amplification.