title: GSM8335943: BP-CTB_1344_3; Homo sapiens; OTHER
library_construction_protocol: Sections were deparaffinized and incubated overnight with the Whole Transcriptomic Assay UV-cleavable biological probes according to manufacturer's instructions. Samples were stained with CONFIRM® anti-EGFR (5B7) Rabbit Monoclonal Primary Antibody  (Ventana #790-4347, 1:20 dilution) and anti-HLA-G (4H48) mouse primary antibody (Abcam #ab52455, 1:1000 dilution) for 2 hours followed by secondary staining for 1h with Alexa Fluor anti-rabbit-647 (Life Technologies, 1:500 dilution), Alexa Fluor anti-mouse-594 (Life Technologies, 1:500 dilution), and SYTO13 (Nanostring, 1:10 dilution) for nuclei staining. Slides were scanned on a GeoMx digital spatial profiler machine r. Based on the morphology markers, areas of interest (AOIs) containing at least EGFR+/HLA-G- 100 nuclei were manually selected. Spatial transcriptomic libraries were prepared with GeoMx reagents according to the manufacturer's protocol - Direct PCR