title: GSM7112616: Vehicle-treated- control mouse 4 [VU_4]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from mouse gingival tissues using Trizol reagent (15596018, Life Technologies), Lysing Matrix D (6913500, MP Biomedicals) tubes and quantified by NanoDrop spectrometer at 260 and 280 nm. Library preparation was performed according to the manufacturer's instructions for the Illumina TruSeq stranded library kit. The libraries were then pooled and sequenced on a NextSeq 2000 sequencer (Illumina).