title: GSM6865255: cancer2, RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction was provided by Cloud-seq Biotech Inc. (Shanghai, China). RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad,CA, USA) according to the manufacturer's instructions.A NanoDrop ND-1000 instrument (Thermo Fisher Scientific, Waltham, MA, USA) was used to measure the RNA concentration of each sample, controlling OD 260/280 values of 1.8-2.1 to ensure good RNA purity. RNA high throughput sequencing was performed by Cloud-Seq Biotech (Shanghai, China). Briefly, total RNA was used for removing the rRNAs with GenSeq® rRNA Removal Kit (GenSeq, Inc.). Then, the rRNA-depleted samples were subjected to library construction with GenSeq® Low Input RNA Library Prep Kit (GenSeq, Inc.) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). Library sequencing was performed on an Illumina Novaseq instrument with 150bp paired end reads.