title: GSM7967536: L845; Homo sapiens; OTHER
library_construction_protocol: cells were resuspended in DNA Quick Extraction solution (Lucigen) to lyse the cells and extract genomic DNA. The cell lysate was incubated at 65 °C for 5 min, then 95 °C for 5 min, and stored at −20 °C. A 25 µl PCR-reaction contained 10 µl of genomic DNA, 0.5 µl 10 µM primer forward, 0.5 µl 10 µM primer reverse,  12.5 µl 2x GoTaq Long PCR Master Mix (Promega) and 1.5 µl H2O. The thermocycler setting consisted of one step at 95 °C for 1 min; followed by 18 cycles at 98°C for  10 s, 65°C for 15 s and 72°C for 15 s (wherein the annealing temperature was decreased by 0.5 °C per cycle); followed by 15 cycles at 98°C for 10 s, 58°C for 15 s and 72°C for 15 s; with one final step at 72°