title: GSM6865926: CO+PB_2847; Mus musculus; ncRNA-Seq
library_construction_protocol: 5ul of RNA was used as input for those replacements that could not be quantified. After adapter ligation, UMIs were introduced in the reverse transcription step. The cDNA was amplified using PCR (19 cycles) and during the PCR complementary dual indices (CDI) were added. After PCR the samples were purified using QMN beads. Library preparation was quality controlled using Fragment Analyzer system by Agilent. Based on quality of the inserts and the concentration measurements the libraries were pooled in equimolar ratios. The library pool(s) were quantified using Qubit. The library pool(s) were then sequenced on a NextSeq550(Illumina Inc.) according to the manufacturer instructions (1x75, 1x8)