title: 16S amplicon seq of groundwater from well H52 incubated with Fricktal rock powder for 18 days, replicate 4
design: Polymerase chain reaction was performed using primer pair Bakt_341F/EMP_926R  (Herlemann et al., 2011; Parada et al., 2016) and HotStarTaq Mastermix (Qiagen, Hilden, Germany) as described in the manuscript (Heinze et al. 2024). Amplicons were purified using NucleoSpin Gel & PCR Clean-Up Kit (Macherey-Nagel, Dren, Germany). The NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Frankfurt, Germany) was used to prepare libraries for amplicon sequencing, following the manufacturers instructions. Amplicons were purified using AMPure XP Beads (Beckman Coulter, Krefeld, Germany), and amplicon sequencing was then carried out in-house on a MiSeq Illumina platform (Illumina, Eindhoven, The Netherlands) with v3 chemistry.