title: GSM7077883: MED-Evs; Homo sapiens; miRNA-Seq
library_construction_protocol: RNA was extracted from bdEVs of eight brain regions by miRNeasy Mini Kit reagents (Qiagen 217004) and Zymo-Spin I Columns (Zymo Research C1003-50) according to the manufacturer's instructions. RNA was resuspended in 40 μL Rnase-free water, and 8 µl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010) according to the manufacturer's protocol.