title: GSM8208537: WT,day2,rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Embryo was staged to proper NC13 to Nc14 before they were placed on 1.5mL microcentrifuge tube caps (3 embryos per sample). Embryos were macerated in 10ul TRIzol™ using sterile an 18 gauge needle. RNA was isolated using Chloroform and phasemaker tubes as described in Bogart, K. and Andrews, J. RNA libraries were made using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® following manufacturer's recommended protocol.