title: RNA-seq of ADE1-1-1 at 28 days
design: For each sample involved in the serial transfer experiment, total RNA was extracted as following procedures. Strains (when saturated) were 1:100 diluted to 15ml fresh SC medium and incubated at 30C with shaking. Cells of 8 ml culture at OD600 = 0.650.7 were harvested. Total RNA of all samples was extracted by QIAGEN RNeasy Plus mini kit (Cat No.74136). The sequencing was performed using the paired-end strategy on Illumina HiSeq platform at Genwiz by standard procedure.