title: Metagenomic assessment of a romanian kefir batch
design: For each DNA sample, the bacterial 16S rRNA gene was amplified using a primer set specific for the V3V4 hypervariable regions (341f: 5TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG3 and 805r: 5TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGACTACNVGGGTWTCTAATCC3), (takahashi 2014) while the fungal internal transcribed spacer (ITS) was amplified using a primer set specific for the ITS1 rDNA region (ITS1f: 5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCTTGGTCATTTAGAGGAAGTAA3 and ITS2r: 5TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGCTGCGTTCTTCATCGATGC3) (white tj 1990)  with overhang Illumina adapters (bold). Targeted libraries were prepared according to Illumina protocols, respectively 16S Metagenomic Sequencing Library Preparation Protocol (Part # 15044223 Rev. B, Illumina, 2013) for bacterial community and Fungal Metagenomic Sequencing Demonstrated Protocol (Document #1000000064940 v01, Illumina, 2019) for fungal community. Sequencing was performed on the Illumina MiSeq (Illumina, San Diego, CA, USA) with the V3 chemistry 600 cycle, 300 bp paired end reads protocol, at the Department of Biology, University of Florence (Italy).