title: GSM7350743: A549, siLuc, Cyt, biol rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: For mRNA-seq following subcellular fractionation, RNAs were obtained using Trizol and Trizol LS. Purified RNAs was digested with DNase I.  For J2 fCLIP-seq, RNAs purified using acid phenol:chloroform extraction and DNA contaminant were digested using DNase I. For mRNA-seq following subcellular fractionation, TruSeq Stranded mRNA Sample Prep Kit (Illumina) was used.  For J2 fCLIP-seq, TruSeq Stranded Total RNA with Ribo-zero H/M/R Gold kit (Illumina) was used. For mRNA-seq following subcellular fractionation, RNA-Seq.  For J2 fCLIP-seq, RIP-Seq.