title: GSM8371817: Input, RA+; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were double crosslinked with 2mM di (N-succinimidyl) glutarate (DSG) followed for 45 minutes at room temperature, followed by 1% formaldehyde for an additional 15 minutes at room temperature. Post-fixation, the cells were lysed and sonicated to fragment the chromatin. The non-sonicated chromatin was then precipitated, and the cell extract was subjected to overnight incubation with specific antibodies at 4°C. Subsequently, DNA purification was carried out using the Qiaquick PCR purification kit (QIAGEN, 28106). Library preparation was performed using the NEBNext®Ultra™ II DNA Library Prep Kit for Illumina. High-throughput sequencing using NextSeq1000. NEBNext Ultra II DNA Library Prep Kit