title: GSM8017445: WT-22_biol rep 2; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: ChIP was performed as previously described (Yang et al., 2020). The paired-end sequencing libraries were prepared from the DNA samples using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina. Briefly, approximately 10ng of DNA was processed for end-repair followed by adaptor ligation as per the kit protocol. The ligated products were subjected to purification using NEBNext Sample purification beads. The purified products were PCR amplified with the index primer followed by AMPureXP bead purification. The PCR enriched libraries were analyzed on 4200 Tape Station system (Agilent Technologies) using D1000 Screen tape as per manufacturer instructions. After obtaining the Qubit concentration for the libraries and the mean peak sizes from Agilent Tape Station profile, the PE illumina libraries were loaded onto Novaseq6000 for cluster generation and sequencing.