title: GSM8212690: RIP-seq_Treatment3; Mus musculus; RIP-Seq
library_construction_protocol: After treatment, the RNA fragments extrated from all the samples were incubated with the bead-linked FLAG antibodies and rotated at 4  C for 4 h. The RNA/antibody complexes are then digested with Proteinase K and the eluted RNA is purified by phenol:chloroform extraction. The data of the six samples are IP, the data for input has not been uploaded.Total RNA was extracted using Trizol reagent RNA libraries for IP samples were then constructed with GenSeq® Low Input RNA Library Prep Kit (GenSeq, Inc.) by following the manufacturer's instructions. Libraries were qualified using Agilent 2100 bioanalyzer (Agilent) and then sequenced in sequencer.