title: GSM6720982: HEK293_5U_2_120; Homo sapiens; OTHER
library_construction_protocol: Harvest cells at different time-points after the transfection (HEK293T:30 min, 75 min, 120 min; SH-SY5Y: 20 min, 40 min, 60 min) and the cells were washed with PBS.  The RNA extraction was according to the instruction of Direct-zol™ RNA MiniPrep Plus kit (Zymo Research). The cDNA was prepared from 2 μg total RNA using SuperScript®IV reverse transcriptase (Thermo Fisher Scientific) with random hexamer, following the manufacturer's protocol. Standard Illumina library construction protocol Amplicon sequencing; The PCR amplicons were subject to Illumina NextSeq 150 paired-end (HEK293T) or single-end (SH-SY5Y) sequencing