title: GSM8440187: W1I_T1_leaf_Water_Infected_1; Solanum lycopersicum; RNA-Seq
library_construction_protocol: One-week after each respective BABA treatment, two leaves from four individual plants were collected and stored in liquid nitrogen. Plants were left to grow and produce red fruit. The first three fruit on all plants were marked and collected. Total RNA was isolated from 100 mg of leaves or fruits samples using  TRIzol (Thermo Fisher; catalogue number 15596026) following manufacturer's specifications. Libraries for RNA expression analysis were prepared by Novogene following their standard protocol. After the QC procedures, mRNA from eukaryotic organisms is enriched using oligo(dT) beads. For prokaryotic organisms or eukaryotic organisms' long-non-coding libraries, rRNA is removed using the Ribo-Zero kit that leaves the mRNA. First, the mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichmen