title: GSM7078262: 3R579-1; Drosophila melanogaster; ATAC-seq
library_construction_protocol: Nuclei were isolated as described in Merrill CB,Pabon MA, Montgomery AB, Rodan AR, Rothenfluh A. (2022)  Optimized assay for transposon-accessible chromatin by sequencing (ATAC-seq) preparation from adult Drosophila melanogaster neurons. Scientific Reports 12: 6043. ATAC-seq libraries were constructed by incubating isolated nuclei with Illumina Tn5 enzyme for 23 min at 37 °C. Tagged DNA was purified using a Qiagen MinElute PCR purification kit. Purified fragments were amplified by PCR using Illumina index primers. Amplified libraries were purified and size-selected using AMPure XP beads.