title: GSM8335648: N_Myo2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the tissues using Trizol (Invitrogen, Carlsbad, CA, USA) according to manual instruction. The reaction system is configured for reverse transcription. First-strand cDNA is synthesized with SMART-SeqII. The reaction system is configured for PCR amplification to process preamplification of cDNAs,Qualified amplified cDNAs use transposon-based method for further library construction