title: 16S V3-V4 amplicon seq potato tubers
design: 16S V3-V4 region PCR with Illumina adapter, amplicon purificatio, FA fragment size check of 22 random samples, Nextera barcode PCR, Ampure XP clean-up, FA fragment size check of 22 random samples, equimolar pooling, Illumina MiSeq 2*250 nt paired end run