title: GSM8122013: SCR_Cut&Tag_1; Homo sapiens; OTHER
library_construction_protocol: DNA fragment of nucleosomes with H3K9me3 modification was extracted from Mina53-KD or control NCM460 cells using anti-H3K9me3 antibody and NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (for Illumina®) DNA sequence is tagmented, with adapters added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries. After the PCR reaction, libraries were purified with the AMPure beads and library quality was assessed on the Agilent Bioanalyzer 2100 system.