title: GSM7784070: A2780, cPVP, biol rep 2; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were washed with PBS and incubated in the 37°C incubator with media containing 200 units/mL DNase (LS002006, Worthington) in 1.2 mM MgCl2 and 0.5 mM CaCl2 for 30 min. ATAC-seq was performed using an ATAC-seq kit (Active motif, 53150) according to manufacturer's protocol. 100,000 cells were taken per reaction. ATAC-seq libraries were purified and size selected with HighPrep PCR beads (AC-60005, MagBio). Two rounds of size selection were performed. In the first, 0.6X beads were added, and the supernatant recovered. In the second, 0.6X beads were added to the supernatant and DNA was eluted from the beads in 10 mM Tris-HCl.