title: GSM6585255: Deug_ovary_polyA; Drosophila eugracilis; RNA-Seq
library_construction_protocol: Ovaries were dissected from 3 -7 days old female flies. Total RNA was extracted from ovaries using TRIZOL. Oligo-dT magnetic beads (NEB) was used to purify polyA+ RNA.  PolyA+ RNA was used to make standard RNA-seq libraries while the total RNA was used to make a Quant-seq mRNA 3' end sequencing library. The RNA-seq library was made using NEB Ultra II Directional RNA library prep kit (NEB), while the mRNA 3' end-seq library was made using the QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen).  For both library constructions, we used the Kapa LongRange DNA polymerase (Sigma) with TruSeq compatible primers to amplify the libraries.