title: GSM8016973: RNA-seq of sheep: methionine1; Ovis aries; RNA-Seq
library_construction_protocol: Lamb hypothalamus RNA was extracted using RNAzol RT (Molecular Research Center; Cincinnati, OH) according to the manufacturer's procedure. The concentration of RNA was measured using UV spectroscopy (Nanodrop Technologies). Sizing, quantitation, integrity, and purity from RNA was were assessed using a BioAnalyzer 2100 and RNA NanoChip assay (Agilent Technologies). Quantified and qualified RNA samples were sent out to BGI for DNBSEQ library construction.