title: Klebsiella pneumoniae
design: K. pneumoniae ATCC43863 was cultured in MH broth at 37, 200 rpm in THZ-100 constant temperature culture shaker (Yiheng, China) until it reached the logarithmic growth phase. The culture was then diluted 1:10 in fresh liquid culture medium supplemented with AW1 (1 MIC), AW2 (25 M), or both peptides. The culture was incubated for 2 h. Subsequently, the bacteria were washed three times with PBS (Gibco, USA)(0.01 mM, pH 7.4), and then precipitated by centrifugation at 7,000 rpm for 10 min at 4. A prokaryotic RNA library was constructed and sequenced by Sango Biotechnology Co., Ltd., following a previously described protocol16. The Ribo-off rRNA Depletion Kit (Vazyme, China) was used to remove rRNA from the total RNA. The VAHTS Stranded mRNA-seq V2 Library Prep Kit for Illumina (Vazyme, China) was used to construct a sequencing library of rRNA-depleted RNA. The purified cDNA library was sequenced using the Illumina Hiseq platform, generating 150 bp paired-end reads.