title: GSM7635209: infected perch spleen 478; Perca fluviatilis; RNA-Seq
library_construction_protocol: Tissue samples were crushed with a steel mortar and pestle to produce a homogenized powder, while frozen spleen tissue was mechanically crushed (the tissue consistency of spleen samples was not conducive to mortar and pestle homogenization) using a Retsch Mixer Mill MM 400 (Retsch). Total RNA was extracted using a NucleoSpin RNA extraction kit (MACHEREY-NAGEL, Duren, Germany). RNA sample concentrations were measured with a NanoDrop 2000 (ThermoFisher), and sample quality was evaluated using a TapeStation 2200 (Agilent Technologies) Sequencing libraries were prepared at the Novogene Cambridge Science Park (Cambridge, United Kingdom) from 300 ng total RNA