title: GSM8073313: WT_skin_3; Mus musculus; RNA-Seq
library_construction_protocol: TSK mice and WT littermates were used for dorsal skin isolation. After shaving the mice, the dorsal skin was removed and processed for RNA isolation using the RNAeasy kit (Qiagen). Samples in RT buffer were submitted to the Technology Center for Genomics & Bioinformatics Core at UCLA to be processed for RNA-Seq. Libraries for RNA-Seq were prepared with KAPA Stranded RNA-Seq Kit with RiboErase Kit. The workflow consisted of depletion of rRNA by hybridization of complementary DNA oligonucleotides, followed by treatment with RNase H and DNase and RNA fragmentaion. First strand cDNA synthesis was done using random priming followed by second strand synthesis to convert cDNA:RNA hybrids to double-stranded cDNA (dscDNA) and incorporate dUTP into the second cDNA strand. cDNA generation was followed by A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane. Sequencing was performed on Illumina HiSeq 3000 for SE 1x50 run.