title: GSM7159374: 5xFAD, 12m, CD11bpositive, ROSnegative, male, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Male and female 12-month-old 5XFAD and NTG mice were perfused with 4°C DPBS and then cortical and hippocampal brain regions were dissected from each animal. Tissues were  incubated with lysis buffer without ActD for 30 min at 37°C. Using a FACSAria II (BD Biosciences), Live Sytox blue–CD11b+ROS– and live Sytox blue–CD11b+ROS+ cell populations were sorted into tubes containing FACS buffer. Sorted cells were resuspended in 4°C DPBS supplemented with 2% FBS and immediately processed for scRNA-seq For scRNA-seq library preparation, live Sytox blue–CD11b+ROS– and live Sytox blue–CD11b+ROS+ sorted cell populations from 5XFAD and NTG brains were processed using the Chromium Single Cell 3' v2 following manufactures instructions (10X Genomics)