title: GSM8035002: Mpdn4mt1-1 Sperm (4mC-AMD-seq); Marchantia polymorpha subsp. ruderalis; OTHER
library_construction_protocol: Sperm were isolated as described previously (Schmid et al., 2018). Sperm DNA was extracted as described (Healey et al., 2014). ATAC-seq sperm libraries were constructed using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits, catalogue number: 20034197, as described (Ding et al., 2021). WT and Mpdn4mt1-1 mutant 4mC-AMD-seq sperm libraries were prepared with the ACCEL-NGS methyl seq DNA library kit (Qiagen, 59104) after treatment of sheared DNA with APOBEC (NEB #E7120S), following manufacturer's instructions. WT and Mpdn4mt1-1 mutant 4mC-TAB-seq sperm libraries were prepared with Ovation Ultralow Methyl-Seq Library Systems (Nugen, 0336) and EpiTect Bisulfite Kit (Qiagen, 59104) according to manufacturer's instructions, with the incorporation of one round of bisulfite conversion. Prior to adaptor ligation, sheared DNA was treated with TET2 (NEB #E7120S) following manufacturer's instructions.