title: GSM8163329: MPRA FIRE SM BV2 cDNA Rep3; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted from the cell with the innuPREP RNA Mini Kit 2.0 (Analytik Jena) , followed by mRNA isolation using the Dynabeads mRNA purification kit (Ambion). cDNA synthesis was performed with the GoScript RT kit and oligo dT primer (Promega). Genomic DNA was extracted with Blood and Tissue kit from Qiagen. Plasmid libraries, extracted genomic DNA and cDNA were prepared for sequencing with two rounds of PCR. The first PCR targets the random barcode present within the transcript. The second PCR adds sequencing indexes to the PCR product. DNA sequencing; targeted RNA-seq