title: GSM8335416: N2, control, rep3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol Reagent (ThermoFisher Scientific) and purified with GeneJET RNA Purification Kit (ThermoFisher Scientific) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. Library construction and sequencing were done by Novogene.