title: GSM8069457: ASC,HFS, H3K4me3, 1; Rattus norvegicus; ChIP-Seq
library_construction_protocol: ASCs and Ads were fixed in 0.8% formaldehyde for 10 minutes at room temperature, followed by 5 minutes with glycine (0.125 mM) as a quenching reagent. Nuclei were isolated from mature adipocytes. ASCs and Ads nuclei were lysated and chromatin was sheared to 300-500 bp using the EpiSonic 1100 equipment Libraries were generated using the NEBnext® Ultratm ii DNA Kit for Illumina with 13 cycles of amplification. For each study time, two libraries were generated, with 3-4 rats each.