title: GSM7865233: RD PD circHIPK3 ODD; Homo sapiens; RNA-Seq
library_construction_protocol: 10-15x106 cells were pelleted and resuspended in 1 ml of cold Lysis Buffer (Tris-HCl pH 7.5 50mM, NaCl 150mM, MgCl2 3mM, NP40 0,5%, EDTA 2mM) supplemented with PIC 1X, RNAse Inhibitor 1:400 and DTT 1mM. Cells were lysed, lysates were subjected to RNA pulldown with biotynilated probes and streptadivine beads and RNA was precipitated through phenol/chloroform extraction. QIAseq miRNA library kit (QIAGEN, Hilden, Germany) has been used for library preparation following the manufacturer's instructions. Libraries were then prepared for sequencing and sequenced on single-end 150 bp mode on NovaSeq 6000 (Illumina, San Diego, CA)