title: GSM8208035: PyMT Fresh Lane6 MULTI; Mus musculus; RNA-Seq
library_construction_protocol: Lungs from PyMT or 4T1 tumor-bearing mice were surgically removed and dissociated using a custom dissociation media containing dispase, dnase I, and collagenase P. Single-cell suspensions were washed with PBS prior to barcoding with MULTI-seq lipid-modified oligonucleotides. Barcoded samples were pooled into BSA-containing PBS and stained with a live-dead stain and CD45, CD31, and Ter119 antibodies prior to FACS isolation of CD45+ CD31- Ter119- lung immune cells. Libraries were prepared according to manufacturers instructions (10x Genomics V3.1) with a separate MULTI-seq library prep workflow performed following cDNA amplification, as described previously (McGinnis, Patterson, et al., Nature Methods, 2019) scRNA-seq with MULTI-seq