title: RNA-seq of human gene editing cells : HSPCs
design: Total RNA was isolated and purified from electroporated and mock cells using TRIzol reagent (Invitrogen, CA, USA) following the manufacturer's procedure. After testing the purity and integrity of the RNA, mRNA sequencing library was constructed following the standard protocol. Sequencing was performed on the Illumina HiSeq X Ten platform with PE156 strategy.