title: GSM6500667: CXCR4 BBZ+antigen rep1; Mus musculus; RNA-Seq
library_construction_protocol: Different T cells were cocultured with antigen-positive tumor cells for four hours and then sorted for RNA-sequencing. Total RNA was extracted from T cells using TRIzolTM Reagent (Invitrogen) and the RNA quality was checked using an Agilent Bioanalyzer 4200 (Agilent technologies). Then, the total RNA was purified with the RNA Clean XP Kit (A63987, Beckman Coulter) and DNase set (79254, QIAGEN). Sequencing libraries were generated using VAHTS® mRNA-seq V2 Library Prep Kit for Illumina (NR603, Vazyme) according to the manufacturer's instructions.