title: GSM8017029: Aged pulp cell, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Mouse mandibles were carefully dissected from 15 C57BL/6 mice aged 8-12 months, which representative of the early stages of aging, using precise dissection under a stereomicroscope. The surrounding bones and soft tissues were carefully removed with scalpels and fine-point tweezers. The incisor pulp, with minimal inclusion of dental epithelium, was isolated, fragmented, and immersed in cold PBS. Subsequently, these fragments were digested in α-MEM (Gibco) containing collagenase at 37°C for 30-45 mins The single cell suspension was quantified and loaded into the 10X Chromium system, targeting approximately10,000 cells for barcoding in preparation for scRNA-seq using a Single Cell 5′ Library Kit (10X Genomics). Cells were successfully barcoded and their transcriptomes were sequenced using the  Illumina Novaseq System (Illumina, San Diego, CA).