title: GSM6579114: scRNA_Embryo; Mus musculus; RNA-Seq
library_construction_protocol: The single-cell suspension was washed three times in 0.4% BSA in 1X PBS by centrifugation at 4 °C for 5 minutes at 135 g in DNA Lobind tubes (0030108035, Eppendorf). The cells were counted with a hemocytometer (using Trypan blue staining), and the overall number was accounted for by counting dead cells as well. The number of input cells was determined following 10X genomics' recommendations for a sample recovery of 4,000 cells. scRNAseq was performed on the cells using a 10X Genomics Chromium Single-cell 3' v2 kit. Except for the number of cycles used, single-cell libraries were generated according to the manufacturer's protocol. For cDNA amplification, 9 cycles in total were used. For library amplification, 8 cycles were used in total. The quality and concentration of cDNA and the library were assessed using a 4200 Agilent TapeStation device and D5000 ScreenTape reagents. 10X Genomic single-cell 3' RNA-seq Accel-NGS Methyl-Seq DNA Library Kit (manufacturer's recommendation)