title: GSM6435582: IDA009_S1; Homo sapiens; RNA-Seq
library_construction_protocol: The tissue obtained from bronchial brushings was treated with 0.25% Trypsin/EDTA for epithelial sheet dissociation and cells were sorted using a BD FACSAria II. FACS was used to isolate singlet events based on forward scatter height vs. forward scatter area (FSH-H vs. FSH-A). Dead cells (PI+) and red blood cells (GYPA/CD235a+) are stained and excluded. For each donor, single live cells (Hoechst 33342+ Prodium Iodide- CD235a-) were sorted into 2 96-well PCR plates per patient sample. Cells were resuspended and sorted into 96-well PCR plates before being processed using the CEL-Seq2 RNA library preparation protocol.