title: GSM7112478: D - 1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Roots were roots were harvested from both +Fe and -Fe conditions for protoplast collection, followed by fluorescence-activated cell sorting in triplicates. RNA was extracted from FACS epidermal protoplast using RNAeasy Plant RNA Purification Kit (Qiagen). RNA libraries were prepared for sequencing by performing cDNA synthesis and amplification using the NEBNext® Poly(A) mRNA Magnetic Isolation Module followed by the NEBNext® Ultra™ II Directional RNA Library Prep and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1)