title: GSM8208193: Liver innate immune cells, mRNA-derived cDNA, lane 1; Mus musculus; RNA-Seq
library_construction_protocol: Liver innate immune cells from ND-fed or CDA-HFD-fed WT and EGR2-KO mice were sorted by FACSAriaIII and stained with different DNA barcode-conjugated PE-streptavidins (BioLegend Totalseq PE-streptavidin (A951, A952, A953, and A954)) to identify each sample origin. Sorted cells were pooled and subject to scRNA-seq using BD RhapsodyTM Single-Cell Analysis System (BD  Biosciences), and resultant cDNA was amplified by TAS-Seq protocol. 3,000 cells /A951 ; 3,000 cells /A952 ; 9,000 cells /A953 ; 9,000 cells /A964 were loaded onto BD RhapsodyTM Single-Cell Analysis System. cDNA library was processed into a sequencing library by using an NEBNext Ultra II FS DNA library prep kit for Illumina (New England Biolabs). Sequencing adapters were added to associated Totalseq PE-streptavidin libraries by PCR. Pooled library concentration was adjusted to 2.0 nM and 12% PhiX control library v3 (Illumina) was spiked into the library.