title: WES of PDX tumor tissues isolated from NSG mice
design: In brief, Xenome was used to classify the origin of sequencing reads and filter out the reads originated from mouse. The remaining sequencing reads were aligned to the reference genome (hg19) using BWA-MEM followed by GATK Best Practices workflow. Somatic mutations were identified using MuTect2 and Pisces and in-house scripts were used to filter out potential false positives. Likely damaging variants were annotated including premature termination, canonical splice site, frameshift insertion/deletion, and missense mutation using Annovar.