title: GSM6205004: Donor1_E2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from EEO and stromal cells using a standard TRIzol-based protocol and Direct-zol RNA MiniPrep Plus isolation kit (Zymo Research, R2070). To eliminate genomic DNA contamination, isolated RNA was treated with DNase I (RNase-Free DNase Set, Qiagen, 79256). Total RNA (500ng) from each sample was reverse transcribed using iScript RT Supermix (Bio-Rad, 1708841). Real-time PCR was performed using SsoAdvanced Universal SYBR Green Supermix