title: GSM8208718: Lung; Mus musculus; RNA-Seq
library_construction_protocol: Lung tissue was fixed with formaldehyde and dissociated with gentleMACS Octo Dissociator (Miltenyi Biotec). After hybridizing the detection probe of the Chromium Fixed RNA Kit for mouse transcriptome (10x Genomix) to intracellular RNA, a ligation reaction was performed to join the hybridized probes. After mixing each sample so that the number of cells is equal, the barcoded primer beads and cells are encapsulated using Chromium Next GEM Chip Q Single Cell Kit on Chromium X (10x Genomix). The probe and primer are annealed, and an extension reaction is performed to create a detection probe. The library was prepared by PCR amplification and purification using indexed primers included in Dual Index Kit TS Set A (10x Genomix).