title: GSM7635621: C57_liver_ad-dyrk1b1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNA extraction kits (LS1040; Promega). The enriched mRNA was fragmented and transcribed into cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (7530, New England Biolabs). The purified double-stranded cDNA fragments were end repaired, 'A' base added, and ligated to Illumina sequencing adapters. The ligation reaction was purified with the AMPure XP Beads and enriched by PCR amplification to create the final cDNA library. The resulting cDNA library was sequenced using Illumina Novaseq 6000.