title: GSM7965645: K4m-2; Mus musculus; ChIP-Seq
library_construction_protocol: Liver biopsies were homogenized with a Dounce in 1 X cold PBS. Sample were then double cross-linked by incubating  30 min with 1 mM disuccinimidyl glutarate, followed by 10 min with 1% formaldehyde. After quenching, nuclei were inreched and Chromatin fragmentation was processed by 20 min incubation at 37°C with 20 gel units MNase. Reaction was stopped by addition of 50 mM EDTA and 2 min incubation on ice.  After 5 min centrifugation at 16,000g (4°C) pellet was resuspended in 50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS, 1 mM DTT and 1X protease inhibitor cocktail and sonicated for 5 min (30 sec ON, 30 sec OFF) with a Bioruptor Plus device. Libraries were generated using MicroPlex Library Preparation kit V2