title: GSM7162874: SKIN_PBS1_citeseq; Mus musculus; RNA-Seq
library_construction_protocol: Dorsal interscapular lesional skin and spleens of 20-week-old MRL/lpr mice were harvested one day after a 5-day treatment course with either PBS (vehicle) or PX-478 at the dose of 30 mg/kg daily. After preparation and staining of single-cell suspensions from skin, live CD45+ and CD45- single cells were sorted and admixed at a 50:50 ratio. These cells and whole splenocytes were then stained for surface protein (Feature Barcoding Technology) per BioLegend protocol with 0.5 mg of each oligonucleotide-tagged TotalSeqTM-C antibody: anti-mouse CD4 (BioLegend, Cat #100571), anti-mouse CD8a (BioLegend, Cat #100785), and anti-mouse CD45RB (BioLegend, Cat #103321). Cells were then submitted immediately for 10X single-cell RNA sequencing (5'v2). Library preparation was performed according to the manufacter's instructions (single cell 5' v2 protocol, 10x Genomics).