title: GSM7635283: Arabidopsis thaliana col-0 La Shoots replicate 3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNAs were extracted and purified from the roots and the shoots using the RNeasy Plant Mini Kit (Qiagen), following the manufacturer's instructions. Genomic DNA was removed using DNAse I (Sigma Aldrich). Total RNAs were quantified spectrophotometrically using a Nanodrop ND-1000 (NanoDrop Technologies), and RNA integrity assessed using a Bioanalyzer 2100 (Agilent, CA, USA). Stranded cDNA libraries (TruSeq-3 library kit) were made using at least 2 µg RNA, and RNA selection using polyA selection. RNA-sequencing was performed using an Illumina HiSeq 2500 instrument, with 2x 125 bp paired-end sequencing.