title: GSM7830997: Cohort A5248, Subject 363044, day 84; Homo sapiens; RNA-Seq
library_construction_protocol: Libraries were prepared for TCR sequencing using the SMARTer Human TCR a/b Profiling Kit v2 (Takara Bio USA, Inc., Cat# 634479). Typically, 30ng total RNA was used as input for cDNA synthesis using Takara's oligo dT primers (TCR SMART UMI Oligo). β-chain repertoire of the TCR variable region was targeted for library construction. Library quantity, purity and size selection were assessed using Qubit fluorometer (Qubit DNA Assay kit, Invitrogen Q32854) and an Agilent Bioanalyzer (D5000 ScreenTape, Agilent 5067-5592) with acceptable library size distribution between 600 to 1150bp. Sample TCR libraries were pooled with 10% PhiX control v3 (Illumina, Cat# FC-110-3001). Sequencing was performed on an Illumina NextSeq2000 sequencer with 2x150 base paired end read chemistry through the UNC High-Throughput  Sequencing Facility.