title: GSM8070564: Retina_06w_S02; Nothobranchius furzeri; RNA-Seq
library_construction_protocol: Fish were euthanized using 0.1% tris buffered tricaine. Retinas were collected in complete L15 (Leibovitz 15 medium complemented with 2% Penicilin-Streptomycin, 0.5% Gentamycin, 2% heat-inactivated Fetal Bovin Serum, 1% Glutamax, 2% MEM Essential Amino Acids, 2% B27, 25 mM D-Glucose, 25 mM HEPES and brought to pH7.4) after retinal pigment epitheluim removal. Retinas were rinsed three times with complete L15 before enzymetic digesting using papain (16 U/ml) for 30 min at 28°C.  Before mechinal tritruation in sterile PBS with 1% bovine serum albumine (fraction V), digested retinas were rinsed three times with complete L15. Cell clumps were removed using a 40 µm cell strainer. scRNA-seq was performed using standard 10x Genomics v3 3' sequencing protocol.