title: GSM8370898: JR9_ALS; Homo sapiens; RNA-Seq
library_construction_protocol: Single nuclei were extracted from archived human postmortem motor cortex tissue using a Dounce homogenizer After removing cell debris, the lysed, filtered homogenate was then promptly centrifuged and supernatant decanted without disrupting the nuclei pellet. Following wash processes, the nucleus suspension was purified via gradient centrifugation, and sorted by FACS. Nuclei were loaded onto 10x Chromium chips, with library preparation performed using the Single Cell 3' Gene Expression kit v3.1 according to the manufacturer's instructions. Single nuclei RNA-seq