title: GSM6924344: mouse_E18.5_mTEC_widetype_rep1; Mus musculus; RNA-Seq
library_construction_protocol: For E18.5 embryoes, six to nine thymi of each genotype (wildtype and Insm1 mutant) were collected and mTECs were isolated from the pool of two to three thymi with the same genotype using UEA1 coupled Dynabeads. Total RNAs were isolated using TRIZOL reagents. For adult mice, mTEC was isolated from each of the thymus using UEA1 coupled Dynabeads. According to NEBNextRUltraTM Directional RNA Library Prep Kit for IlluminaR (NEB, USA). Three sequencing libraries for each genotype were generated