title: GSM6500591: Plasma miRNA-seq at ET14 of BP YFC-10 [YFC-10-3]; Homo sapiens; miRNA-Seq
library_construction_protocol: The miRNAs were extracted from the plasma using the mirVana™ miRNA Isolation Kit (Cat #. AM1561, Austin TX, USA) according to the manufacturer's protocol. The concentration and quality of RNA samples were determined by NanoDrop ND-1000 spectrophotometer (Thermo Fisher, Wilmington, DE, USA) and 2100 Bioanalyzer Instruments (Agilent Technologies, Santa Clara, CA, USA). The miRNA sequencing library was constructed using the QIAseq miRNA Library Kit (QIAGEN, German). The total RNA of each sample was used to prepare the miRNA sequencing library as the following steps: 3'-adaptor ligation, 5'-adaptor ligation, cDNA synthesis, PCR amplification, and gel purification. After quantification with Qubit (Thermo Fisher, USA), the libraries were captured on cBOT (Illumina, USA) to be amplified in situ as clusters following cBot User Guide.