title: GSM8208710: NSC_p53_RNAseq_2; Mus musculus; RNA-Seq
library_construction_protocol: Samples were lysed and extracted using the Trizol reagent After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA with RIN values of 8.4-9.9 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on NovaSeq 6000 in a PE100 run, using the NovaSeq 6000 S2 Reagent Kit (200 Cycles) (Illumina). An average of 33 million paired reads was generated per sample. Ribosomal reads represented 0.4-1.7% of the total reads generated and the percent of mRNA bases averaged 91%.