title: DNA-Seq of Pseudomonas putida strain PD689_tt_L1
design: Illumina-based sequencing, the 500-bp sequencing library was prepared with axGen DNA Lib Prep EZ (Integrated DNA Technologies) according to manufacturer'sinstructions. In brief, 500 ng total DNA has been enzymatically fragmented for8 minutes. CS_UMI Adapter (Integrated DNA Technologies) and dual indexingprimers were used to adapt the library. The library was amplified for 8 PCRcycles. After QC and pooling, the library was sequenced using Illumina Nextseqinstrument with 300 cycles mid output chemistry. Illumina reads were trimmedand filtered using Trimmomatic v0.38.1 with the sliding window model usingaverage quality required 20 (Bolger 2014).