title: MicroRNA  of above ground tissues (stem and leaf)
design: Briefly, First of all, ligated the 3 SR and 5 SR Adaptor. Then, reverse transcription synthetic first chain. Last, PCR amplification and Size Selection. PAGE gel was used to electrophoresis fragment screening purposes, rubber cutting recycling as the pieces get small RNA libraries. At last, PCR products were purified (AMPure XP system) and library quality was assessed.