title: GSM8073295: C&R, D907, H3K9me3, Control, Rep2; Drosophila melanogaster; OTHER
library_construction_protocol: 20 female third-instar larval wing discs were dissected in Schneider medium at room temperature. CUT&RUN assays were performed according to Kami Ahmad's protocol implemented for Drosophila tissues (https://dx.doi.org/10.17504/protocols.io.umfeu3n) DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). Adaptors were diluted 1:10 in water for adaptor ligation (step 2). The size selection of the adaptor-ligated DNA in step 3A was omitted (we proceeded directly to step 3B). We performed 14 cycles of PCR with 10 seconds of annealing/extension for enrichment of short DNA fragments. Libraries were sequenced on a NovaSeq 6000 system with 150 bp PE reads