title: GSM8122678: pre_pat13_ACT_1; Homo sapiens; RNA-Seq
library_construction_protocol: Tumor RNA extraction was performed using the E.Z.N.A. FFPE RNA kit (Omega Biotek). The concentration and integrity of the extracted total RNA were estimated using the Quant-iT™ RiboGreen® RNA Assay Kit (Thermo Fisher Scientific) and the Fragment Analyzer Systems using the RNA High Sense chip, respectively. Up to 30ng of RNA with DV200>20% was used to prepare libraries using SMRTer Stranded Total RNAseq v2 (Clonetech) with slight modifications.