title: GSM8033685: Z002KB_fresh; Homo sapiens; RNA-Seq
library_construction_protocol: Bronchial cells were dissociated from the bronchial airway brushes from 2 asthmatic children using Bacillus licheniformis cold active protease (10mg/ml), EDTA (0.5mM), and EGTA (0.5mM) at 4°C with vortex mixing, followed by enzyme neutralization with FBS.  Red blood cell lysis was performed and cells were washed twice in 0.04% BSA/PBS. Targeted capture of 8000 cells was performed using the 10X Genomics Chromium Next GEM Single Cell 3' reagent kit chemistry.  Sample capture, cDNA synthesis, and library preparation was performed following 10X Genomics Chromium Next GEM Single Cell 3' chemistry (CG000204 Rev D), and barcoded samples were pooled and sequenced on an Illumina NovaSeq 6000.