title: GSM8209342: RNA Mir-146 KO/Empty 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the sorted GFP+/lin-/ckit+/Sca+ cells cells using RNeasy Plus Micro Kit (Qiagen). The initial amplification step for all samples was done with the NuGEN Ovation RNA-Seq System v2. The assay was used to amplify RNA samples to create double stranded cDNA. The concentrations were measured using the Qubit dsDNA BR assay. Libraries were then created for all samples using the Illumina protocol (Nextera XT DNA Sample Preparation Kit). The concentrations were measured using the Qubit dsDNA HS assay. The size of the libraries for each sample was measured using the Agilent HS DNA chip. The concentration of the pool was optimized to acquire at least 15-20 million reads per sample.