title: GSM8210414: S-V9-Ab E0771 bio rep 2; Mus musculus; OTHER
library_construction_protocol: Fresh tumor tissues were removed and placed in a petri-dish and embedded with room temperature OCT without any bubbles on the tissue's surface. The embedded samples were transferred into the cryo mold . The cryo mold containing OCT-embedded samples were put into the metal beaker with 2-methylbutane in a dewar of liquid nitrogen until the OCT was solidified. Sample cryosectioning, affixment to cDNA capture slide, H&E staining, tissue permeabilization, RNA capture, and cNDA synthesis were performed according to the 10 x Visium spatial transcriptomics' introductions. Libaries were prepared with 10x Genomics Visium Spatial Tissue Optimization Slides & Reagent Kit 4 slides(product code:1000193), Visium Spatial Gene Expression Slides & Reagent Kit 4 rxns(product code:1000187), and Dual index KitTT Set A 96 rxns(product code:1000215) Spatial Transcriptomics