title: GSM8335823: H23 PTPRH Overexpression Replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit (Qiagen #74104) according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was synthesized  using random hexamer primers, followed by the second strand cDNA  synthesis using either dUTP for directional library. For the directional library, it was ready after end repair, A-tailing, adapter  ligation, size selection, USER enzyme digestion, amplification, and  purification. Paired-end RNA sequencing (Illumina PE150) were conducted using NovaSeq X Plus Series (Illumina) with a minimum coverage of 30M reads per sample