title: GSM7159393: Fgg390-396A, CD11b, F, 6m, scRNAseq, rep4; Mus musculus; RNA-Seq
library_construction_protocol: Brains from male and female 6-month-old 5XFAD, 5XFAD:Fggg390-396A, NTG and Fggg390-396A mice were processed for FACS. Live Sytox blue–CD11b+ cells from cortical/hippocampal tissues were sorted into tubes containing DPBS supplemented with 5% FBS at 4°C and then were resuspended in 4°C DPBS supplemented with 2% FBS at 333 cells mL-1 and processed for scRNA-seq. For scRNA-seq library preparation, live Sytox blue–CD11b+ cells were processed with the Chromium Single Cell 3' v3 kit following manufactures instructions (10X Genomics).