title: GSM6661503: MixedABMR2; Homo sapiens; RNA-Seq
library_construction_protocol: NK cells were FACS-sorted from frozen PBMC, with the following gating strategy: viable cells, CD3 negative, CD19 negative, CD14 negative, CD7 positive, CD56 positive. Samples were labeled with TotalSeq-A Cell Hashing HTO Ab (BioLegend), then resuspended in PBS+0.04% BSA. Ready-to sequence Illumina libraries were generated using the Chromium Single Cell 3′ Reagent Kit (v3 Chemistry; 10× Genomics; Pleasanton, CA) per manufacturer's protocol. Single Cell chips were loaded to retrieve 3000 cells. Sequencing single-indexed libraries (5 nM) were pooled. The resulting pooled library was loaded on a NovaSeq S2-100. The following parameters of sequencing were used: Read 1: 28 cycles; i7 Index 8 cycles; Read 2: 91 cycles, as specified by the 10× Genomics guidelines.