title: GSM8017487: CUTLL1, shELK3, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent following the manufacturer's procedure. The total RNA quality and quantity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >8.0. The poly (A) mRNA was purified from the total RNA using the Dynabeads mRNA purification kit (Invitrogen). RNA libraries were then constructed using the NEB Next Ultra Directional RNA Library Prep Kit (NEB)