title: GSM8163931: E14 KO, replicate 3, run 1, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: For E14 samples, the forebrains were dissected and flash frozen in liquid nitrogen. The tissue was dissociated by dounce homogenization, filtered through a 40um filter, and washed twice with Nuclei Wash Buffer (DPBS, 1% BSA, and 0.2U/ul RNase Inhibitor). The nuclei were counted and 500k-2.5M nuclei were fixed using the ScaleBio Sample Fixation Kit. The nuclei were stored at -80C until all samples have been collected and fixed. The libraries were made according to manufacturer's instructions for Scale Biosciences' Single Cell RNA Kit.