title: GSM6435614: LH_0034; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were initially dissociated from two nasal swabs obtained from the inferior turbinate. The cells were then washed with Phosphate-buffered saline (PBS, Sigma Aldrich, Burlington, MA) and dissociated to single-cell suspensions using 0.25% Trypsin/EDTA (Thermo Fisher, Waltham, MA). The red blood cells were removed after being treated in the 1X RBC Lysis Buffer (Thermo Fisher, Waltham, MA) for 2 minutes. Trypan blue (STEMCELL, Vancouver, BC, Canada) was used for measuring cell viability. The final concentration of the cells was measured using a hemocytometer under a light microscope before library preparation using the 10X Genomics Platform (Pleasanton, CA). Samples with over 85% viability were prepared for single-cell sequencing using the 10X Genomics Platform.