title: GSM8440280: h-hADSCs_3,  scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Freshly collected h-hADSCs and c-hADSCs were separately digested, then washed in PBS twice and resuspended in PBS with 0.04% w/v BSA. Library was performed according to the manufacter's instructions (single cell 3' V2 protocol, 10x Genomics). Briefly, hADSCs were resuspended in the master mix and loaded together with patiti0ning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The oply-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an llumina R! primer sequence. Unique Molecular Identifier (UMI) and the 10X Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR and apropiated sized fragments were selected with SPRIselect library construction. During the library construction llumina R2 primer sequence, paired-ended constructs with P5 and P7 sequence and a sample index were added