title: GSM6435750: C6F5UE-C6F(L)V-induced MEFs, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions were prepared in PBS (HyClone) at a density of 1×10^5 cells/mL and loaded onto microfluidic devices. The GEXSCOPE® Single-Cell RNA Library Kit (Singleron Biotechnologies) was used to create single-cell RNA-seq libraries according to the Singleron GEXSCOPE® procedure (Singleron Biotechnologies). Individual libraries were diluted to a concentration of 4 nM before they were pooled for sequencing. Pooled libraries were sequenced with 150 bp paired end reads on an Illumina sequencer.