title: GSM8213048: choroid plexus, normal, sample 4; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen tissue was thawed in ice cold wash buffer (.32 M sucrose, 5mM CaCl2, 3mM magnesium acetate, 2.0 mM EGTA, 10mM Tris-HCl pH 8.0) + .001% Triton-X and 1mM DTT. Thawed tissue fragments were transferred to a glass dounce on ice and given 10 strokes each with an A-type pestle and a B-type pestle. The resulting nuclei suspension was sequentially filtered through 100 and 40 um filters and pelleted by centrifugation for 5 minutes at 555xg and 4°C. Pellets were washed by resuspension in wash buffer and centrifugation 2-4 times. After the final wash, pellets were resuspended in sterile filtered nuclei storage buffer (0.43 M sucrose, 70mM KCl, 2mM MgCl2, 10mM Tris-HCl pH7.2, 5mM EGTA). Single nucleus expression libraries were made from 5-10K nuclei using the Chromium Single Cell 3' Library & Gel Bead kit v3.1 (10x Genomics) according to the manufacturer's protocol. snRNA-seq