title: GSM6924910: 19.12 46-1_3669; Picea abies; Bisulfite-Seq
library_construction_protocol: Total DNA was extracted from powdered tissue of several terminal buds detached from twigs without needles and some bud scales and grinded in liquid nitrogen with mortar and pestle. Total DNA was extracted using DNeasy Plant Mini Kit (Qiagen, Hilden, Germany, cat. no. 69104), according to the manufacturer's instruction. After DNA-shearing using a M220 Focused-ultrasonicator (Covaris, Inc., Woburn, MA, US), the DNA fragments were bisulfite-converted using the EZ DNA Methylation-Gold Kit (Zymo Research Corp, Orange, CA, USA) and the libraries were made using SeqCap Epi Enrichment system (Roche, Basel, Switzerland), following the manufacturer´s instructions. Targeted bisulfite sequencing