title: GSM6862244: Stem cell-derived islets, Sample B; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy® Plus Micro Kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.