title: GSM8371601: B16F1 tumor 2, day 21; Mus musculus; RNA-Seq
library_construction_protocol: Frozen pieces of tumors (approximately 5-8 mg) were mechanically disrupted with metallic beads on TissueLyser LT (Qiagen, Netherlands). Then, total RNA was extracted from tumor samples using NucleoSpin RNAII kit (Macherery-Nagel). mRNA-seq libraries were generated according to the manufacturer's instructions of the TruSeq stranded mRNA Library Prep kit (20020595, Illumina)