title: GSM6925169: YY1L: young isochronic parabiont 1 left; Mus musculus; RNA-Seq
library_construction_protocol: Brain cells were processed through all steps to generate stable cDNA libraries. Briefly, after dissociation, cells were diluted in ice-cold PBS containing 0.4% BSA at a density of 1,000 cells/ul. For every sample, 17,400 cells were loaded into a Chromium Single Cell™ Chip (10x Genomics) and processed following the manufacturer™s instructions. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3™ Library & Gel Bead kit v2 and i7 Mutiplex kit (10X Genomics). Libraries were pooled based on their molar concentrations. Pooled libraries were then loaded at 2.07 pM and sequenced on a NextSeq 500 instrument (Illumina) with 26 bases for read1, 57 bases for read2 and 8 bases for Index1.