title: GSM6862108: CT-vIII, 5900-αCD40, Day 0, Replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction, library preparations, and sequencing reactions were conducted at GENEWIZ, LLC. (South Plainfield, NJ, USA) as follows: Total RNA was extracted from flash-frozen brain tumor tissue samples collected post-therapy and 3 days post-therapy (N=3/group/time point) using Qiagen RNeasy Plus Universal mini kit following the manufacturer's instructions (Qiagen). mRNAs were first enriched with Oligo(dT) beads and RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following the manufacturer's instructions (NEB).