title: GSM8033417: s76004; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from thoracic aorta samples with Qiagen columns (RNeasy MiniSpin Columns) using a polytron (T25 basic, IKA, Labortechnik) RNA concentrations were obtained using nanodrop or a fluorometric Qubit RNA assay (Life Technologies, Grand Island, New York, USA). The quality of the RNA (RNA integrity number) was determined on the Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA) as per the manufacturer's instructions. RNA sequencing 344 libraries were prepared from 100 ng of total mRNA using the Illumina® Stranded Total RNA 345 Prep