title: GSM8206899: Hepatic_7wpi; Ovis aries; miRNA-Seq
library_construction_protocol: Total RNA was harvested from 6 replicates for each timpoint using TriReagent RT LS (Molecular Research Centre) according to the manufactureres instructions. A total of 800uL of starting serum was used for Total RNA extraction. Total RNA for each timepoint was pooled for small RNA sequneincg (representing 6 animals per timepoint). For construction of sequencing libraries 1ug of total RNA was used for small RNA seq. RNA libraries for small RNA seq were prepared using TruSeq Small RNA library prep kit.