title: GSM6436149: Patient Tissue 26628_4849_S; Homo sapiens; RNA-Seq
library_construction_protocol: NEBNext Ultra II Directional RNA-Seq Sample Preparation with rRNA Depletion protocol (p/n E7760S/L, version 1.0, April 2017) using 100, 50, or 2.5ng RNA input, along with ThermoFisher's ERCC RNA Spike-In Control Mixes Protocol (publication number 4455352, rev. D). PDX tumor were harvested from mice and trimmed of excess fat pad tissue. Representative tumor fragments were snap frozen in liquid nitrogen. Tumor was homogenized and RNA extracted using the RNeasy Mini Kit from Qiagen.