title: GSM8209511: CC_S2_13; Solanum lycopersicum; RNA-Seq
library_construction_protocol: Total RNA was extracted from leaves using the Spectrum Plant Total RNA Kit (Sigma Aldrich). After digestion of contaminant DNA by DNAse I (ThermoScientific) at 37°C for 30 min, RNA quantity and quality were determined with a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, United States). cDNA libraries were prepared from 1 µg total RNA using NEBnext Ultra TM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer's recommendations. A total of 21 libraries (three each for wt and SL- leaves, plus wt treated with GR245DS) were constructed and quantified using a Qubit 2.9 fluorometer (Life Technologies) and sequenced on an Illumina platform to generate paired-end reads.