title: GSM8122198: MEC-16; human metagenome; RNA-Seq
library_construction_protocol: The nucleic acid was extracted by TGuide S96 magnetic bead method soil/feces genomic DNA extraction kit. Remarks: Manufacturer: Tiangen Biochemical Technology (Beijing) Co., Ltd., model: DP812. Extraction kit: TGUIDE S96 Magnetic SOIL/STOOL DNA Kit (Tiangen Biotechnology (Beijing) Co., Ltd., China) DNA was isolated from the specimens and processed for microbiome analysis by 16S rRNA gene sequencing. Genomic DNA was extracted using a PowerSoil® DNA Isolation Kit following the manufacturer's instructions. The extracted total DNA was subjected to PCR using specific primers with barcodes synthesised based on the full-length primer sequences (27F: 5'-AGRGTTTGATYNTGGCTCAG-3' and 1492R:5'- TASGGHTACCTTGTTASGACTT-3'). The PCR conditions were as follows: 95 °C for 5 min, 25/30 cycles of 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 60 s/1 kb, and 72 °C for 7 min). The PCR products were purified, quantified, and homogenised to form a sequencing library (SMRT Bell).