title: GSM6923529: s3925_cbl; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was homogenized using a B2X24B Bullet Blender and 0.5 mm glass beads as described by the manufacturer (Next Advance, Troy, NY).  RNA was isolated using the RNeasy Lipid Tissue Mini Kit with on-column DNase digestion using the RNase-free DNase Set (Qiagen, Valencia, CA, Cat #74804 and #79254). RNA-seq libraries were prepared using Illumina TruSeq mRNA Library Prep Kit with TruSeq Unique Dual Indexed adapters (Illumina, San Diego, CA) and were quantified with Qubit 1X dsDNA High-Sensitivity NGS Gel Kit (Thermo Fisher Scientific). KAPA Library Quantification (Roche, Basel, Switzerland) was used for further library characterization and Agilent Fragment Analyzer with the High-sensitivity NGS Gel Kit (Agilent) was used for determining fragment size