title: GSM6426624: blood that underwent CTC enichment [2019.05.01_HDM64_IFD_042619]; Homo sapiens; OTHER
library_construction_protocol: Blood samples from either healthy donors or patients with localized or metastatic prostate cancer were subjected to CTC-ichip enrichment (10^4-fold leukocyte depletion). The enriched CTCs (ranging from 0.1% to 1% of residual leukocytes) were subjected to high molecule weight (HMW) DNA extraction using the HMW DNA extraction kit (QIAGEN). High molecule weight (HMW) DNA was used to prepare sequencing library for Oxford Nanopore sequencing using the rapid barcoding kit (Nanopore#SQK-RBK004). For each sequencing run, 11 blood samples (either from healthy donors or cancer patients), together with 1 lambda DNA (unmethylated control), were uniquely barcoded and pooled together. Sequencing was performed using a Nanopore MinION device with R9.4 flowcell for 48 h, per manufacturer instructions.