title: GSM8122928: CS19; Homo sapiens; RNA-Seq
library_construction_protocol: All tissues for sequencing and spatial work were collected in HypoThermosol FRS preservation solution (H4416-100ML Merck) and stored at 4 °C until processing. Tissue dissociation was conducted within 24 h of tissue retrieval.Tissues were cut into <1 mm3 segments before being digested with Trypsin/EDTA 0.25% phenol red (25200072 Thermo Fisher Scientific) for 10-15 min at 37 °C with intermittent shaking. Digested tissue was passed through a 100 µm filter and cells collected by centrifugation (500g for 5 min at 4 °C). Cells were washed with PBS and then resuspended in PBS 0.04% BSA before cell counting. cells were loaded according to the manufacturer's protocol for the Chromium Next GEM Single Cell 5 v2 (Dual index) kit for CS17 amnion and Chromium Next GEM Single Cell 3 v3.1 (Dual index) kit for CS19 and CS22 amnion from 10X Genomics to attain 7,000 cells per reaction. Library preparation was carried out according to the manufacturer's protocol. Libraries were sequenced, aiming at a minimum coverage of 20,000 raw reads per cell, on the Illumina HiSeq4000 or Novaseq 6000 systems using the sequencing format: read 1, 26 cycles; i7 index, 8 cycles, i5 index, 0 cycles; read 2, 98 cycles.