title: GSM8371443: Donor 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from 4-5 skin sections per skin sample with 90-180 cells (melanocytes) per section and amplified using the Ovation® RNA-Seq System V2 (NuGEN). Amplified cDNA was sheared using a Covaris shearing device to obtain approximately 300bp sized fragments. Afterwards rRNA depletion and library preparation were performed using an adapted protocol of the Trio RNA-Seq kit (NuGEN) for low-input samples.