title: GSM6500325: DMSO Ser5P ChIP-seq Rep 1; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked with formaldehyde for 5 minutes and quenched with glycine. After washing, cell pellets were resuspended in sonication buffer and sonicated using the Qsonica Q800R3 system. INTS11Halo chromatin was spiked with Drosophila chromatin prior to IP. Chromatin was pre-cleared with Protein A agarose and incubated with the indicated antibody overnight. Protein A agarose beads were added and allowed to bind antibody-epitope complexes. IPs were washed extensively and eluted twice. DNA was purified using phenol/chloroform. All libraries were constructed with the NEB NEBNext Ultra II DNA Library kit .