title: GSM7949220: Idiopathic Pulmonary Fibrosis 1; Homo sapiens; RNA-Seq
library_construction_protocol: Briefly, around 10,000 cells were targeted per sample and loaded onto each well of a Chromium Single Cell G Chip to generate single cell emulsions primed for reverse transcription.  After breaking the emulsion, the single cell specific barcoded DNAs were subjected to cDNA amplification and QC on the Agilent 4200 TapeStation Instrument, using the Agilent D5000 kit.  Each sample cDNA was used to prepare indexed libraries that were pooled prior to sequencing.  A quality control run was performed on the Illumina Miseq using the nano 300 cycle kit (1.4 Million reads/run), to estimate the number of targeted cells per sample. The cell estimate enabled the core to re-balance the pooled sample prior to deep sequencing. The generation of single cell indexed libraries, from the thawed COVID PF and IPF PBMCs, was performed by the School of Medicine Genome Analysis and Technology Core, RRID:SCR_018883, using the 10X Genomics chromium controller platform and the Chromium Single Cell 5′ Library & Gel Bead Kit v1.1 reagent.  Deep sequencing was performed on either the NGS Illumina NextSeq 500 using the 150 cycle kit or NextSeq 2000 using the P3-100 cycle kit.  Control samples were sequenced on the MGISEQ-2000.