title: Inflamed pulp tissue 1
design: The library construction of RNA-seq is mainly divided into the following steps: Total RNA extraction;   Perform first strand cDNA synthesis;   Proceed immediately to second strand cDNA synthesis;   Purifiy double-stranded cDNA using DNA clean beads;   End prep of cDNA library;   Adaptor ligation;   Purifiy the Adaptor Ligated DNA Using Beads;   PCR Enrichment of Adaptor Ligated DNA;   Purification of amplified DNA;   Sequencing. Na-seq data after disembarkation, first assess the quality of the original data using fastqc software; Then STAR software was used to compare the original data to the reference genome. Then, cuffdiff software was used to calculate the expression level of each sample, and the genes were annotated for subsequent analysis.