title: GSM8371168: HNRNPC, HNRNPM, Re-CLIP, HEK293T, Rep 2; Homo sapiens; OTHER
library_construction_protocol: For irCLIPv2, immunoprecipitation (IP) followed by 3'ligation. For Re-CLIP, 1st IP, 3' ligation, IP destruction and reconstitution, 2nd IP. Then, ligated RNPs were resolved on SDS-page, transferred to nitrocellulose, and RNA was recovered after protein K digestion using streptavidin beads. On-beads 1st strand cDNA synthesis, 3' cDNA-tailing, 2nd strand synthesis, and amplification.