title: GSM6435674: WT CD8 TILs, biological replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: 8 weeks old WT and Susd2-/- C57BL/6 mice were inoculated subcutaneously with 1 x 10^6 MC38 tumor cells. On Day 18 post inoculation, single cell suspensions were prepared from the tumors after mechanical disruption and enzymatic digestion with 1 mg/mL Type IV Collagenase. Cells were strained through a 70 µM filter. Live tumor infiltrating lymphocytes (CD45+ 7-AAD-) were sorted on a BD FACSAria Ilu Cell Sorter and immediately processed for scRNA-seq. Experimental procedures for scRNA-seq followed established techniques using the Chromium Single Cell 3' Library V3 Kit (10x Genomics).  Briefly, FACS-sorted Live CD45+ cells were processed using the inDrops V3 scRNA-seq platform. inDrops Libraries were sequenced on the NextSeq Illumina Platform, paired-end mode.The raw sequences (FASTQ format; four WT and four KO) were aligned and quantified using the CellRanger (v3.0.2) pipeline against the pre-built 10X mouse reference genome (mm10)