title: AMPLICON of Severe acute respiratory syndrome coronavirus 2: Human 21-CoV-1284-VTM2-AXRV763
design: The 21-CoV-1284-VTM2-AXRV763 library was prepared using the QIAseq DIRECT SARS-CoV-2 Library Kit (QIAGEN) in accordance with manufacturer's recommendations. Briefly, 5uL of RNA was reverse transcribed followed by amplification of cDNA targets using the DIRECT SARS-CoV-2 Primer Panel (QIAGEN) with 31 cycles of PCR and cleaned-up by magnetic bead purification. The amplicons were then amplified and indexed using 7 cycles of PCR. Final libraries were cleaned-up by magnetic bead purification and quantified using fluorescent-based assays including PicoGreen (Life Technologies) or Qubit Fluorometer (Invitrogen), and Fragment Analyzer (Advanced Analytics). Final libraries were sequenced on Illumina NovaSeq 6000 sequencer with 2x150bp reads.