title: RNA-Seq on triple negative breast cancer tumor.
design: Total RNA was extracted using the NORGEN Total RNA Purification Kit and treated with DNase I.  RNA was purified using the AMPure XP beads.  cDNA was prepared using the Ovation RNA-Seq System V2 and then shared using the Covaris E220 sonicator.  Library was prepared using the Agilent SureSelect XT Low Input Reagent Kit.  The libraries were then hybridized to Agilent SureSelect Human All Exon v.4 probes.