title: GSM8033733: pbmc use stage; Homo sapiens; RNA-Seq
library_construction_protocol: 5 mL of blood was collected from the patients who develop paroxysmal　atrial fibril-lation after using crizotinib . PBMCs were prepared using the density gradient cen-trifugation method. In brief, whole blood cells were diluted with 1× DPBS (1:1 ratio), layered on top of Ficoll-Paque Plus, and then centrifuged at 500g for 20 minutes at room temperature (RT). The white layer containing PBMCs was isolated and washed twice with DPBS. Subsequently, erythrocytes were removed using red blood cell lysate on ice. PBMCs were prepared using the density gradient centrifugation method at 1000 rpm for 5 minutes. The cells were successfully resuspended and filtered through a 40 μm cell strainer. The cell suspensions underwent library preparation using the 10× Chromium Single Cell 3′ library kit and the Chromium Single Cell 30 v3 reagent (from 10× Ge-nomics) immediately after preparation.