title: GSM8208041: Col-0 pistil grown at hAT - replicate 2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Gynoecium samples from flowers at stages 11 and 12 (before anthesis) were collected from wild type, phyb and 35S::PIF4 plants grown at nAT and hAT. Total RNA was extracted from 100 mg of ovules using the RNeasy Plant Mini Kit (Qiagen) following the manufacturer's protocol. RNA isolates were treated with rDNAse Macherey Nagel) to remove traces of contaminant DNA and purified using a RNeasy MinElute Cleanup Kit (Qiagen). mRNAseq libraries were constructed by Novogene, starting with 100 ng of high-quality RNA per sample. mRNA purification was performed using oligo(dT) attached magnetic beads, followed by fragmentation and first-strand cDNA synthesis. Second strand cDNA synthesis, end repair, adapter ligation, and size selection were performed. PCR enrichment yielded in the final cDNA library.