title: GSM6500821: TSA-SIN3HA-2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: After overnight incubation, phenol-chloroform extractions and EtOH precipitations were conducted to purify ChIP-DNA. The ChIP-DNA was eluted in a total volume of 200 μL double-distilled water. ChIP-seq libraries were prepared using Swift S2 Acel reagents on a Beckman Coulter Biomek i7 liquid handling platform from approximately 1 ng of DNA according to manufacturer's protocol and 14 cycles of PCR amplification.