title: GSM7784084: S2003S0748; Homo sapiens; RNA-Seq
library_construction_protocol: Ribosomal RNA material was reduced using the QIAseq FastSelect –rRNA HMR Kit to remove cytoplasmic and mitochondrial rRNAs and QIAseq FastSelect –5S/16S/23S Kit to remove pan bacterial rRNAs (Qiagen, Germantown, MD, USA). RNA was sheared to 300-400 bp. Prior to PCR amplification, cDNA fragments were ligated to adapters for Illumina TruSeq UD Indexed adapters (Illumina Inc, San Diego CA, USA). Libraries were prepared by the Van Andel Genomics Core from 500 ng of total RNA using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, MA USA).