title: GSM7794636: CD4T, KO RNA rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from cells based on chloroform-phenol/guanidine. Total RNA prepared with rRNA depletion and PolyA RNA was isolated by oligo dT beads. cDNA is amplified through 8-11 cycles of PCR amplification using the NEBNext Ultra RNA Library Prep Kit for Illumina. Libraries were paired-end sequenced on Illumina HiSeq X ten platform.