title: GSM7784074: A2780, cPVP, biol rep 2; Homo sapiens; OTHER
library_construction_protocol: Cells were harvested by scraping into ice-cold PBS. DNA was extracted by using DNeasy® Blood & Tissue kit (69504, Qiagen), and quantified by the QuantiFluor® dsDNA System (E2670, Promega). 2 µg of each DNA sample were taken for library preparation. Damage-seq was performed as previously described by Hu et al. (PNAS, 2017) without biotin purification after the primer extension step (Jiang et al., ACS Central Science, 2023). Briefly, Genomic DNA was sheared by sonication with Biotuptor Pico sonicator (Diagenode Inc) to generate fragments averaging 300 bp in length. Damaged DNA immunoprecipitation was performed as described previously, with anti-cisplatin modified DNA antibody (ab103261, Abcam).