title: GSM7699248: GROseq_wildtype_rep2; Drosophila melanogaster; OTHER
library_construction_protocol: After running the nuclear run-on reaction the RNA was extracted by TRIzol extraction and subjected to streptovidin pull-down and extracted by Phenol:Chloroform purification. For purified RNA 3' and 5' adapters were added and purified by sptreptovidin beads. The RNA was reverse transcribed and cDNA was amplified.