title: Whole genome sequencing of Mycobacterium intracellulare B1-4
design: A sequence of nucleotides incorporated by the DNA polymerase while reading a template, such as a circular SMRTbell (TM) template. Polymerase reads are most useful for quality control of the instrument run. Polymerase read metrics primarily reflect movie length and other run parameters rather than insert size distribution. Polymerase reads are trimmed to include only the high-quality region; they include sequences from adapters; and can further include sequence from multiple passes around a circular template. Each polymerase read is partitioned to form one or more subreads, which contain sequence from a single pass of a polymerase on a single strand of an insert within a SMRTbell (TM) template and no adapter sequences. The subreads contain the full set of quality values and kinetic measurements. Subreads are useful for de novo assembly.