title: GSM6659280: PBS nonstim, replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: On the 22nd day from the first cisplatin/GENUS treatment, samples were anesthetized with isofluoren and cardiac perfused with ice cold DPBS (Thermo Fisher ScientificScienctific, 14190235). Two hippocampi per animal were isolated and four hippocampi from two animals were homogenized using adult neural tissue dissociation kit (Miltenyi bio, 130-092-628). The resulting single cell suspension was processed to generate a cDNA library with targeted recovery of 5000 cells using 10x Genomics Chromium™ Single Cell 3' Library & Gel Bead Kit v3.1 (1000215, 1000268, 1000127). The library was sequenced with NovaSeq 6000 (Illumina) and NovaSeq S4 Flow Cell at MIT bioinformatics core.