title: GSM8072554: Seedling, 3d at 4°C, H3K27me3, replicate 1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: For each replicate, 1g of seedlings was crosslinked with PFA and chromatin was extracted as described in Vyse et al. (2020). The chromatin was immunoprecipitated using 1 μg of α-H3K27me3 (C15410195 Diagenode) or α-H3K4me3 (C15410003, Diagenode). DNA was purified and concentrated using the ChIP DNA Clean and Concentrator lit (Zymo Research). The libraries were prepared using the ThruPLEX DNA-seq kit (Takara Bio) and indexes from the SMARTer DNA HT Dual Index kit (Takara Bio). DNA fragments were then selected based on size using AMPure beds (Beckman Coulter). The concentration of the samples was measured using the Qubit dsDNA High Sensitivity kit and the Qubit Fluorometer (ThermoFisher Scientific) and the library quality was assessed using the High Sensitivity DNA ScreenTape and the TapeStation (Agilent).