title: GSM8035498: Patient 4, timepoint 3, scTCR-seq; Homo sapiens; OTHER
library_construction_protocol: Frozen cells were resuspended using 1xPBS solution and then filtered through a 40 µm filter. A small portion of the cell solution was mixed with AOPI and placed on a cell counting slide to check the viability (LUNA-FL™ Automated Fluorescence Cell Counter). Cells with viability over 70% were used for sequencing. scRNA-seq: Chromium Next GEM Single Cell 5' Kit v2 and Chromium Next GEM Chip K Single Cell Kit were used to generate barcoded 5' single cell library (10X Genomics Chromium Instrument). Each cDNA library that passed the quality control (Agilent Bioanalyzer) was sequenced on an Illumina NovaSeq6000 and NextSeq500. scV(D)J-seq: TCR and BCR sequencing was performed using cDNA synthesized by the nested-PCR enrichment method. TCR and BCR libraries were generated using the Chromium Single Cell Human TCR and BCR Amplification Kit (10X Genomics), and each library was checked for quality control (Agilent Bioanalyzer) before sequencing.