title: GSM7112110: TET2-KO_B16-OVA_PBS_3-2_Biol Rep 3; Mus musculus; RNA-Seq
library_construction_protocol: After treatment, WT or TET2-KO B16 tumor tissue were harvested and digested with Collagenase + DNAase to make single cell suspensions in DMEM medium. 10,000 single cells fromt these tumors were isolated for single-cell sequencing under 10X genomics platform. RNA libraries for RNA-seq were prepared using Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics)  and the Illumina NextSeq2000 system was used for sequencing following manufacturer's protocols.