title: GSM8122525: Donor 2 AOI 64; Homo sapiens; OTHER
library_construction_protocol: Selected slides were deparaffinized and rehydrated, followed by antigen retrieval for 15 minutes at 90C in Tris-EDTA. RNA targets were exposed using proteinase K (1 ug/ml) in PBS for 15 minutes at 37C. For in situ hybridization, GeoMx reagents and protocols were used according to the manufacturer's instructions. Slides were stained with morphology markers prior to RNA collection. Slides were covered with Buffer S (Nanostring) and loaded into the GeoMx DSP instrument. Regions of interest were defined by the user and RNA molecules which were affixed by photocleavable linkers are released using programmable UV light then collected into microtiter wells for sequencing. After pooling, purification, and quality control process the library was then sequenced on an Illumina NGS platform.