title: GSM8163186: NK cells, RNA+0.5h; Homo sapiens; RNA-Seq
library_construction_protocol: 0.5h or 6h after the addition of RNA, cells were FACS-sorted to isolate NK cells (CD56+CD3-) and CD8+T cells. Isolated cells were immediately placed into Trizol (Thermo) reagent to obtain total RNA. Library preparation for RNA-Seq was performed with TAKARA SMART-Seq® HT PLUS Kit (Takara Bio Inc., Shiga, Japan). A part (0.4-1.4 ng) of total RNAs were reverse transcribed and amplified by 12 PCR cycles. Then, 3 ng of each cDNA was subjected to fragmentation, illumina index attachment to the ends, and PCR-amplified (15 cycles).