title: RNA-Seq to evaluate kinetics of changes to transcriptome of Acanthamoeba ATCC 30461 exposed to contact lens materials.
design: Samples were DNAse treated with Invitrogen DNAse (RNAse free). Library preparation was performed using Illuminas Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp IDT for Illumina indices. Sequencing was done on a NextSeq2000 giving 2x50bp reads.