title: GSM8212812: JSC15-3 5-FU day-14 (2); Homo sapiens; RNA-Seq
library_construction_protocol: (1) JSC18-1 cells were treated with or without 5-FU at IC50 values, 1 μM for 5 days, and (2) JSC15-3 and JSC18-1 cells were treated with or without 5-FU at IC50 values, 1 μM and 0.5 μM for 14 days, and (3) their xenograft tumors in BALB/c-nu mice were treated with PBS or 5-FU (100 mg/kg). RNA was extracted using RNeasy mini kit (Qiagen) according to the manufacturer's instructions. Sequence libraries were constructed with TruSeq stranded mRNA library preparation kit(Illumina) according to the manufacture's instructions.