title: GSM7078013: B6_Ly49DnHn_ampliconBS; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genome DNA was extracted using Quick-DNA Microprep Kit (Zymo Research, R3020). WGBS: DNA was sheared to produce 350 bp DNA fragments. 100 ng of DNA fragments were then bisulfite converted using EZ DNA Methylation-Gold Kit (Zymo, D5005) for subsequent WGBS library construction, with xGen Methyl-Seq DNA Library Prep Kit (IDT, 10009860). Amplicon bisulfite: 500ng of genomic DNA was bisulfite-converted using EZ DNA Methylation-Gold Kit (Zymo Research, D5005). The bisulfite-converted DNA was then amplified with target-specific primers using ZymoTaq PreMix (Zymo Research, E2003). Amplicon libraries were then generated using NEBNext Ultra II DNA Library Prep Kit (NEB, E7645).