title: GSM8163694: Choroid plexus brain organoids, A1_Mock; Homo sapiens; RNA-Seq
library_construction_protocol: Briefly, 8 or more  organoids per condition (mock and HIV infection) were washed twice with DPBS without ions. A single-cell suspension was generated using neural dissociation kit (Miltenyi Biotec) and gentleMACS™ dissociator according to manufacturer's protocol. Microglia were enriched using CD11b magnetic microbeads (Miltentyi Biotec). Cells were resuspended in an appropriate volume of 0.04% BSA in PBS. Viability and cell numbers were assessed before being subjected to the 10X RNA-sequencing protocol. Single-cell suspensions per condition were loaded into a Chromium Next GEM Chip G, with a target output of 5000~10000 cells per channel. The chip was then loaded onto a chromium controller v3.1 (l0X Genomics), for single-cell GEM generation and barcoding. For the six loaded channels, GEM generation, barcoding, cDNA amplification and library preparation were performed using the Single-cell 3′ Gel Bead and Library v3.1 kit (10X Genomics) in the BSL3. Amplified cDNA and final libraries were evaluated on a Bioanalyzer for quality control and sequenced together on lllumina NovaSeq 6000 platform.