title: GSM6968057: Hemocytes, Dietary Restriction, Control, rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted using the standard phenol-isopropanol-chloroform protocol. cDNA synthesis was done by the two-step Smart-seq3 protocol (Hagemann-Jensen et al., 2020). Amplification was done by their provided protocol as well but using LATaq Polymerase. Library preparation was done using an adaptation of the protocol from Picelli et al., 2014