title: GSM7784049: Tumor, mteth.IL15.IL21_good response, #12; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Freshly collected spleens or tumors were processed using Papain Dissociation System (Worthington Biochemical Corporation) according to manufacturer's protocol. In brief, each specimen were minced in the papain solution containing DNase and incubated in 37 degrees in a CO2 incubator for 30 minutes. The cloudy supernatant was collected, and was layered on top of Earle's Balanced Salt Solution containing 10% of albumin-ovomucoid inhibitor. The samples were centrifuged, and the pelleted cells were resuspended in PBS. Tumor #83 and #93 were submitted for scRNA-seq as is. All the other samples were incubated with the hIL-15 PE-conjugated antibody, and given TotalSeq™-C0951 PE Streptavidin. Spleen samples were also hashtagged as follows. Splenocyte #12: TotalSeq-C0251, barcode seq. GTCAACTCTTTAGCG; #17: TotalSeq-C0252, barcode seq. TGATGGCCTATTGGG; #18: TotalSeq-C0253, barcode seq. TTCCGCCTCTCTTTG; Splenocyte #83: TotalSeq-C0251, barcode seq. GTCAACTCTTTAGCG; #93; TotalSeq-C0252, barcode seq. TGATGGCCTATTGGG; #87; TotalSeq-C0253, barcode seq. TTCCGCCTCTCTTTG. #12, #17, and #18 were combined into one sample, and #83, #93, and #87 were combined into one sample.