title: GSM8370904: Day 2 blastoids hypoxia; Mus musculus; RNA-Seq
library_construction_protocol: Natural embryos were recovered as described above. Using a 96-well plate, embryos were washed twice in DEPC treated PBS, and then moved to dissociation mix: Ebioscience Accumax Cell Aggregate Dissociation Medium (00-4666-56, Thermo Scientific) supplemented with 1:1000 DNase to remove aberrant DNA released from lysed cells. Embryos were left in dissociation media at room temperature for 25-60 minutes with trituration by mouth pipetting and glass capillaries every 10 minutes. The dissociation reaction was quenched using equal parts FC media. Cells were passed through a 20 µM filter and spun down at 450 rcf. Cells were then resuspended in 0.04% BSA-PBS according to the 10X Chromium Next GEM single cell 3' Kit v3.1 user guide. Chromium scRNA-seq workflow