title: GSM6424516: Sample_RNA-Lib_Act-Aac11-IR; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Wandering stage (L3 instar) larvae were sorted for GFP expression under a dissecting microscope equipped with a Zeiss HBO lamp and a GFP filter. Larvae were rinsed in 1x PBS and then  immediately ground and  dissolved in Trizol (TM) kept on ice. Tissue+Trizol mixtures were frozen at -80 degrees Celsius. This was repeated until 10-20 larvae per genotype (Act>Aac11-RNAi or Act>control) had been collected. Samples were then pooled within genotypes as needed, and total RNA was extracted according the manufacturer's instructions and depleted for rRNA prior to analysis. 4 µg of total RNA was rRNA depleted and utilized for library construction with the Illumina TruSeq RNA Sample Preparation Kit v2 following the manufacturer's instructions. Libraries were sequenced with an Illumina HiScan platform.