title: GSM7865482: L2 48h NT; Homo sapiens; RNA-Seq
library_construction_protocol: PCLSs were enzymatically and mechanically dissociated. Single cell suspension was used for the 10X encapsulation. Cells were counted and the targeted cell number for loading was 8000 cells per sample. 10X encapsulation and library construction were done using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 per the manufacturer's instruction. Multi-seq library preparation was done as previously described.  Libraries were mixed at an approximate 10:1 molar ratio of gene expression to LMO barcodes for sequencing.  The sequencing was done on the Illumina NovaSeq 6000 using 10X Genomics recommended sequencing parameters.