title: GSM6923316: VS14GAMM; Homo sapiens; RNA-Seq
library_construction_protocol: Total mRNA was extracted from blood samples (3 mL) using TempusTM spin RNA isolation kit (Thermofisher). For each blood sample, Ig mRNA was reverse-transcribed to cDNA by a 5'RACE PCR with consensus reverse primers for IgH Cμ, Cɣ and Cα constant region and a cap race primer carrying unique molecular identifiers (UMIs), amplified by PCR using a universal forward primer and a nested reverse primer selected within the CH1 exon of the Cμ, Cɣ and Cα genes and sequenced with an Illumina MiSeq, standard Illumina sequencing primers and MiSeq Reagent Kit v3 (600 cycles).