title: GSM8163554: H1_KO_I1_r2; Mus musculus; ChIP-Seq
library_construction_protocol: Testes were homogenized and fixed with 4% formaldehyde for 15 minutes. After quenching, cell suspension was washed and lysed to obtain a nuclei suspension. The nuclei suspension was filtered througth a 40µm cell strainer and sonicated. After a high speed of centrifugation, chromatin was collected and incubated with the antibody and then DNA-protein complexes were isolated. Enriched DNA were prepared using the Next Gen DNA Library kit from Active Motif  following manufacturer's instructions. For DMC1, enriched DNA were prepared using the Illumina Trueseq protocol with an additional step of kinetic enrichment to titrate ssDNA.