title: GSM8033646: Young Fischer344 rat RPE/choroids-01; Rattus norvegicus; RNA-Seq
library_construction_protocol: Each eye ball was opened by removing the cornea, iris and lens, and the neural retina was firstly isolated and put into RNAzol immediately and flash frozen in liquid nitrogen. After the neural retina being taken, RPE/choroids were isolated by washing the remaining eye cup with RNA zol and flash frozed in liquid nitrogen. Total RNA was isolated follwing manufacturer's instructions of RNAzol (Sigma-Aldrich, Saint-Louis, MO). Only samples with RNA integrity number (RIN) > 7 was selected for poly-A captured mRNA library preparation. To compare old vs. young and 8-AG treated aged vs untreated aged neural retinae, mRNA libraries were prepared using poly-A capturation (N=3).  For RNA from RPE/choroids, due to low RNA yield,  mRNA lirbaries were genereated using polyA selection with enrichment for full-length transcripts.