title: GSM8371278: Chondrocyte stimulated by IL-1β alone 3; Homo sapiens; RNA-Seq
library_construction_protocol: Chondrocytes RNA was purified using TRIzol Reagent and RNA extraction kit (NucleoSpin®) according to the manufacturere's protocol Libraries were generated from 1 µg of high-quality RNA using NEBNext Ultra II Directional RNA Library Prep Kit  .Data of 150 bp× 2 paired-end of 4-6G bases per sample (3 samples for each condition) was trimmed by fastp and mapped by alignment to hg38 reference genome using STAR. The reads were quantified using RSEM and the raw count data were subjected to Subio Platorm Plug-ins software.