title: GSM7784076: XR_A2780, cisplatin, biol rep 2; Homo sapiens; OTHER
library_construction_protocol: 390x106 cells per library were harvested and collected by centrifugation. Cells were resuspended in lysis buffer and lysed using syringes sequentially with 25 and 27 G needles. XR-seq was performed as previously described (Hu. et al., PNAS, 2016). Briefly, primary excision products were pulled-down by TFIIH co-immunoprecipitation using anti-p62 antibody (Santa-Cruz Biotechnology, sc-293) and anti-p89 antibody (Santa-Cruz Biotechnology, sc-292). Adapters were ligated on both ends of the excision oligomer and immunoprecipitated using cisplatin-specific antibody (abcam, 103261). Cisplatin-induced damages were reversed in-vitro by incubating the samples with 200 mM NaCN at 65°C overnight. DNA was PCR amplified and purified by native polyacrylamide gel electrophoresis (PAGE) to create the library.