title: GSM8072882: Culex pallen, siILP1 (ILP1 knockdown mosquitoes) bio rep 1; Culex pipiens pallens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Trizol method following the manufucturers instructions 1 μg of total RNA was subjected to library synthesis using the TruSeq RNA Library Preparation Kit (Illumina). Three independent biological replicates of each group were performed, giving three RNA-seq libraries per group. 1 μg of total RNA was enriched for the Poly-A mRNA and reverse transcribed to double-stranded cDNA. The cDNA was end repaired, adenylated, and ligated with Illumina indexes prior to 12 cycles of PCR.