title: GSM7698734: siSMARCA1#1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from cells was isolated by TRIzol Reagent (Invitrogen) and then purified using the RNeasyMini Kit (Qiagen).The concentration and integrity of total RNA were estimated using a Qubit 2.0 Fluorometer (Invitrogen) and an Agilent 2100 Bioanalyzer (Agilent Technologies), respectively. For RNA-seq, the NEBNext DNA Library Prep Master Mix Set for Illumina (New England BioLabs) was used to prepare a next-generation sequencing expression library following the manufacturer's protocol.