title: GSM7634392: Control 4; Drosophila melanogaster; RNA-Seq
library_construction_protocol: whole RNA from dissected trachea was extracted using RNAmagic (Bio-Budget Technologies GmbH, Germany) Samples were quantified with a fluorometric dye (Quant-IT, Thermofisher, USA) and between 187 and 500 ng per sample were used as input for theTruSeq stranded mRNA library kit (Illumina, USA) following the manufacturers manual. Resulting libraries showed a fragment size distribution of around 350 bp and were sequenced on a HiSeq 4000 (Illumina, USA) with 50bp single-end reads