title: GSM8163398: Blastoids_PIPseq_nontarg_c2_R2; Homo sapiens; RNA-Seq
library_construction_protocol: Single cells were obtained by resuspending blastoids in Collagenase IV (StemCell Technologies, 07909) and incubating at 37 degrees with mild agitation for 40 minutes. Blastoids were centrifuged again in N2B27 media and the pellet was resuspended in 0.5% Trypsin-EDTA (Fisher Scientific, 15-400-054) and incubated for 10 minutes at 37 degrees. Two further washes were performed with N2B27 and the dissociated cells were passed through a 40 um Flowmi cell strainer. Libraries were constructed using the PIP-seq T20 3' Single Cell RNA Kit