title: PacBio Whole-genome sequencing of Pseudoliparis sp.
design: Genomic DNA was extracted from muscle tissue sample using the Qiagen Genomic-tip kit. The DNA was sheared to ~20 kb fragments with a g-TUBE device, purified using AMPure PB beads, and SMRTbell libraries were constructed following PacBio's protocols. The libraries were sequenced on a PacBio RS II using P6-C4 chemistry with 240-minute movie collection times per SMRT cell, and data were processed to generate continuous long reads and high-fidelity reads.