title: GSM8033511: 6hr_rpt5 RNA; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was harvested from five replicates for each timepoint using RNAzol (Molecular Research Centre) according to manufactureres instructions. For construction of sequencing libraries, 2ug of totoal RNA was use for miRNA-seq and 1.5ug total RNA for RNA seq. RNA libraries for RNA-seq were prepared using Illumina Stranded Total RNA prep with RiboZero Plus kit (35M reads/sample) and for miRSeq using Qiaseq miRNA prep kit (75M reads/sample)  following manufacturers insctructions.