title: GSM6720214: AHb1-4; Mus musculus; RNA-Seq
library_construction_protocol: Mouse primary sorted cells were isolated using the previously described protocol, passed through a 40uM filter, and stained for Epcam (clone 9C4, Biolegend), AnnexinV (Biogend), and DAPI using the AnnexinV staining protocol and buffers. Epcam+/AnnexinV-/DAPI- cells were then sorted and GFP or TdTomato was analyzed when applicable. For mouse 2D cells, cells were stained with Epcam and DAPI to exclude irradiated feeders and dead cells. For organoid sorting, DAPI was used to exclude dead cells. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).