title: Salmonella Bacteriophage
design: Whole genome library preparation was performed using Sequencing Ligation kit (SQK-LSK109) and Native barcoding kit (EXP-NBD096) from Oxford Nanopore Technologies. A total of 600 ng of purified  DNA (as quantified by Qubit) from each sample was end-repaired and cleaned up with 1x AmPure beads. Native barcode ligation was performed with NEB blunt/ TA ligase and cleaned with 1x AmPure beads. Barcode sequences are detailed in the table 2. Qubit quantified barcode ligated DNA samples were pooled at Equimolar concentration to attain ~500 ng pooled sample. Further sequencing adapter ligation (AMII) for 10 minutes at 20C using NEBnext Quick Ligation Module was performed. Library mix was cleaned up using 0.4X AmPure beads and library was eluted in 15 l of elution buffer.