title: GSM8017563: Primary freshly isolated BM-MSCs from donor MLDCryo03; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified from sorted and ex-vivo expanded MSCs using the RNeasy Micro Kit (QIAGEN). DNase treatment was performed using RNase-free DNAse Set (QIAGEN), according to the manufacturer's instructions. RNA was quantified with Nanodrop (Thermo Fisher) and its quality assessed by a 2100 Agilent Bioanalyser (Agilent Tech-nologies). Minimum quality was defined as RNA integrity number (RIN) > 8. 300 ng of total RNA were used for library preparation with TruSeq Stranded mRNA (Illumina)