title: GSM7111420: H9C2, Hypoxia, Trim 3 2; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA from H9C2 was extracted using TRIzol reagent (Invitrogen, Thermo Fisher Scientific Inc, Singapore) followed by DNase I treatment. RNA quality was determined by Nanodrop and Bioanalyzer. The RNA-sequencing was performed by the non-stranded poly-A library method. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. This was followed library construction. The library was checked with Qubit and real-time PCR for quantification and Bioanalyzer for size distribution detection. Quantified libraries would be sequenced on Illumina platforms, according to effective library concentration and data amount.