title: GSM8072410: PBMC, Abuse + neglect, rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs were isolated by density gradient over Ficoll-Hypaque (GE Healthcare BioSciences) and RNA was purified using Total RNA purification Kit (Norgen Biotek). RNA quantity was determined using a NanoDrop UV–VIS spectrophotometer  and quality checked via bioanalyzer assay Libraries were prepared from 100ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen GmbH). Libraries were sequenced on a NovaSeq 6000 sequencing system using an S1, 100 cycles flow cell (Illumina Inc.)