title: GSM7645392: E14TG2a，NANOG-AID-Nascent,2h,rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using TRI Reagent. 200 μg of total RNA was used for nascent RNA enrichment and library construction. RNA libraries for nascent RNA-seq were constructed using small RNA library construction strategy. Briefly, RNA was ligated with pre-adenylated 3'-adapter and reverse-transcribed. cDNA was isolated to ligate another adapter. Finally, the RNA library was enriched by PCR amplification.