title: 16S amplicons: control feed pellets
design: gDNA was extracted using Qiagen DNeasy kit, the V4 region of the 16S rRNA gene was amplified with library construction following (Caporaso et al., 2011), PCR products were then purified using Qiagen QIAquick, pooled, and sent for Illumina MiSeq 250bp paired-end sequencing on 500x cycle