title: 16S  metagenetic of reproductive tract: S182
design: DNA extraction was done using the DNeasy Blood & Tissue kit. Genomic DNA was PCR-amplified targeting the V4 region of the 16S rRNA gene using 515F and 806R primers, followed by purification and normalization. A second PCR with barcoded primers from the Access Array Barcode Library for Illumina Sequences was conducted, and the products were pooled. The libraries were then sequenced on the Illumina MiSeq platform.