title: GSM7634782: truB KO, rep3; Escherichia coli; RNA-Seq
library_construction_protocol: Cells were  opened in 300 µL of a cell opening buffer (100 mM Tris, 10 mM EDTA, 0.5% SDS, 286 mM β-mercaptoethanol) containing 10 mg/mL lysozyme, 0.1 U/µL DNase I, and 0.1 U/µL RiboLock RNase inhibitor (ThermoFisher)  and incubated at 37 °C for 5 minutes. Subsequently, Proteinase K was added to a final concentration of 0.5 mg/mL and lysates were incubated at 50 °C for 10 minutes. RNA extractions were performed using TRIzol per the manufacturer's instructions. Strand-specific RNA-seq (2x150 bp, single index) with rRNA depletion (bacteria) library preparation was performed by Azenta.