title: GSM6865657: 2_D6_V5; Mus musculus; RNA-Seq
library_construction_protocol: RNAseq. RNA was extracted using RNeasy mini kit (Qiagen) and treated with DNaseI (Qiagen). Quantseq libraries were prepared with 250 ng RNA using QuantSEQ 3' mRNA-Seq Library Prep Kit FWD for Illumina (015.96, Lexogen) according to manufacturer's recommendations. The libraries were pooled and sequenced with a 50-base-pair single-end protocol on an Illumina HiSeq 2500 sequencer. ChIPseq libraries were generated with 5-10ng DNA using the NEBNext Ultra II DNA library preparation master mix (New England Biolabs, E7645) according to the manufacturer's instructions.