title: GSM8163954: P21 Het, male, replicate 2, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: For P21 samples, cortices from mice were dissected and flash frozen in liquid nitrogen. The tissue was dissociated by dounce homogenization and a gradient centrifugation step using 25:35 Iodixanol and centrifuged at 10,000 xg for 30 min at 4C was performed to purify the nuclei from cellular debris and myelin generated during tissue dissociation. The nuclei were carefully collected from the interface, filtered through a 40um filter, washed with Nuclei Wash Buffer (DPBS, 1% BSA, and 0.1U/ul RNase Inhibitor), and 500k-2.5M nuclei were fixed using the ScaleBio Sample Fixation Kit. The nuclei were stored at -80C until all samples have been collected and fixed. 3 replicates each of male and female MYT1L WT and Het mice were included in this experiment. Libraries were made using the Scale Biosciences Single Cell RNA Kit. The libraries were made according to manufacturer's instructions for Scale Biosciences' Single Cell RNA Kit.