title: GSM7698819: rn-18; Rattus norvegicus; RNA-Seq
library_construction_protocol: Whole-brain tissue was collected from resting-state Wistar rats (Charles-Rivers, n=27) during the early part of their active period. Animals were swiftly decapitated after being transferred directly from their home-cage, their brains were removed, then cooled and washed in ice-cold saline. A 0.5 mm coronal section between Bregma 2.0 and 1.5 mm was sliced in a cooled slice matrix. Bilateral medial prefrontal cortex samples were dissected on ice as defined by the forceps minor of corpus callosum and the medial wall of the hemisphere (i.e. including the infralimbic, prelimbic and anterior cingulate cortices). Samples were immediately placed in Eppendorf tubes on dry ice and stored on -80C temperature until RNA isolation. Whole transcriptome-analysis via RNASeq was performed on the homogenised mPFC tissue. TRUseq (Illumina)