title: Illumina NovaSeq 6000 paired end sequencing; Single-cell RNA-seq of H9 nEnd cells subjected to mesoderm differentiation.
design: Single-cell RNA-seq of H9 nEnd cells subjected to mesoderm differentiation.
library_construction_protocol: The mesoderm differentiation protocol used in this dataset is a combination of published protocols that were further modified. These will be detailed in the corresponding publication. Cells were detached and singularized with TrypLE Select and frozen in KnockOut Serum Replacement supplemented with 10 % DMSO.  Prior to library preparation cells were thawed in DMEM/F-12 with 10 % KnockOut Serum Replacement. 10x Genomics 3' v3.1 kit 10x Genomics 3' v3.1 kit; library preparation was performed by Single Cell Discoveries (Utrecht Netherlands).