title: GSM8033324: AcrIIA4 single-mutant library fraction 1 replicate 3 [AcrDMS_v2_17]; Escherichia coli DH5[alpha]; OTHER
library_construction_protocol: Plasmid purification from E. coli Amplikon sequencing library. Acr region was amplified from purified plasmids using primers with partial Illumina adapter overhang followed by gel purification of DNA fragments. Library preparation was performed by PCR barcoding of DNA fragments using the Nextera XT Index Kit v2 (Illumina).