title: GSM8371886: iPax3 re-isolated, control, rep6; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was performed in two different ways. For samples with high input (in vitro cells), iPax3 and iPax3-N1OE myogenic progenitors RNA was extracted using Trizol (Invitrogen) and RNA extraction Kit (Invitrogen) following manufacturer's instructions. For samples with low input of RNA (in vivo cells, re-isolated donor-derived satellite cells), RNA was extracted using Buffer RLT (QIAGEN) and nucleic acid beads. For samples with high input (in vitro cells), iPax3 and iPax3-N1OE myogenic progenitors. The total RNA was used to for generate unique dual-indexed (UDI) TruSeq stranded mRNA libraries. For samples with low input (in vivo cells, re-isolated donor-derived satellite cells). The cDNA was synthesized using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification kit (New England Biolabs) and the libraries were generated with the Nextera XT DNA Library Preparation Kit (Illumina).