title: GSM7795337: BDC3_50kPa_2; Homo sapiens; ATAC-seq
library_construction_protocol: Omni-ATAC-seq protocol was used to minimize mitochondrial reads from the preps, however no trypsinization was used and instead on-plate disruption/removal of nuclei (using the digitonin present in the lysis buffer) was used to better preserve nuclear mechanical context and connectivity prior to transposition by the Tn5. The Omni-ATAC-seq protocol was used to minimize mitochondrial reads from the preps, however no trypsinization was used and instead on-plate disruption/removal of nuclei (using the digitonin present in the lysis buffer) was used to better preserve nuclear mechanical context and connectivity prior to transposition by the Tn5.  Following the final PCR, libraries were cleaned with a 0.5x/1.8x double-sided SPRI clean. Libraries were subjected to quality control by determining the number of cycles required to reach 25% of the peak threshold in the diagnostic PCR, as well as running the amplified libraries out on a High Sensitivity D1000 Tapestation (Agilent) to confirm expected size, and Qubit dsDNA HS assays were performed to determine a final concentration. Libraries were individually diluted to 6nM and then pooled at equal volumes prior to sequencing on an Illumina HiSeq 4000 using a single lane of 50bp single end reads.