title: GSM7110798: VacV-infected skin (2); Mus musculus; RNA-Seq
library_construction_protocol: Whole ear skin was homogenized in 1 ml of Trizol and RNA was extracted according to the manufacturers protocol. RNA was further purified using the RNeasy Mini Kit (Qiagen). RNA integrity was determined by running samples on the Bioanalyzer (Agilent). Libraries were prepared using the TruSeq Stranded mRNA kit according to the manufacturers protocol (Illumina) Libraries were quantified for sequencing using an NGS Library Quantification Kit (Roche/Kapa Biosystems) on a StepOnePlus Real Time PCR Workstation (Thermo/ABI). Sequencing was done on a NovaSeq 6000 (Illumina).