title: GSM7635722: AKPS_Smad4_2_A3723; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from cell lines using the DNeasy Blood & Tissue Kit (Qiagen). Purified DNA samples were fragmented by restriction digestion with MspI. Following digestion, Klenow Fragment was utilized to create 3'A overhangs. DNAs were subsequently column purified, and then ligated to Methylated Illumina PE Adapters. Ampure SPRI beads were then used to purify products prior to Sodium Bisulfite Converted and amplification to generate libraries.