title: GSM6756952: green stage tomato_control_3; Solanum lycopersicum; miRNA-Seq
library_construction_protocol: Prior to extract total RNA using RNAiso Plus (#9108; Takara), polysaccharides and polyphenols were eliminated with Fruit-mate (#9192; Takara, Shiga, Japan) according to the manufacturer's protocol. After DNase I treatment, the extracted RNA was purified again using an RNeasy Mini Kit (Qiagen, Hilden, Germany).240ng of total RNA was used for the construction of sequencing libraries. Libraries for small RNA sequencing were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Massachusetts, USA), following the manufacturer's protocol.