title: GSM8208359: Cortex, stroke zone 1, immunoprecipitated RNA, rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Immunoprecipitation was performed according to Sanz et al, PNAS 2009. RNA was harvested using RNeasy Micro Plus kit (Qiagen) cDNA and library preparation were prepared using Ovation RNA-Seq System V2 (NuGEN) and KAPA Hyper Prep Kit (KAPA Biosystems) following manufacturer's protocols. 500 ng of generated cDNA were first fragmented to 200bp using Covaris E210 (Covaris) and then used for library preparation following manufacturer's protocols. Libraries were pooled with equal molars for PE 50-bp sequencing on HiSeq 2500 (Illumina).