title: GSM6500690: UMUC3 cells, sgSTIL 6; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cells using trizol reagent (Invitrogen) and then reverse tran-scription was performed using the cDNA Reverse Transcription Kit (Toyobo Life Science, Shanghai China) to synthesize cDNA. 1 ug of total RNA was used for the construction of sequencing libraries. In this project, we sequenced 9 samples used BGISEQ platform, averagely generating about 1.19G Gb bases per sample. The average mapping ratio with reference genome is 88.20%, the average mapping ratio with gene is 81.59%; 16886 genes were identified.