title: GSM8209365: BM, Mature B, Ubxn3b KO, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Mature B subset total RNA was harvested using RNAeasy mini plus kit (Qiagen). 400ng of total RNA was used for the construction of sequencing libraries mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.