title: GSM8035609: WT_CDK1as_Halo_CAP-H2_G2_Input (biol rep1)_ChIP-Seq; Gallus gallus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenchng and cell lysis, chromatin was shear using Diagenode Biorupter. ChIP-seq libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB #E7645) AND NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB #E7335). 15ng of template was used for library preparation for input and IP samples and amplified using 11 cycle on the thermocycler. Libraries were size selected at 200bp to 500bp length range using SPRIselect bead from Beckman Coulter. Purified libraries were analysed by Agilent 2100 Bioanalyzer by using Agilent High Sensitivity DNA Kit (#5067-4626).