title: GSM8212667: RNA-seq_WT1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent Total RNA was extracted followed by library preparation according to Illumina standard instruction (VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina® ). Agilent 4200 bioanalyzer was employed to evaluate the concentration and size distribution of cDNA library before sequencing with an Illumina novaseq6000. The protocol of high-throughput sequencing was fully according to the manufacturer's instructions (Illumina).