title: GSM8017201: BPC195T; Homo sapiens; RNA-Seq
library_construction_protocol: EUS-FNAB from primary tumors were performed prior to any treatment using 19 to 22 G FNA needles (Wilson Cook, Ireland; Boston Scientific, USA as previously described. The material for cytopathology was reclaimed by the use of a dedicated stylet and was instantly fixed in formalin. The remaining material was obtained by air blushing the FNA needle (so-called EUS-FNAB wash – EUS-FNAB-W) and immediately collected in 500 µl of RNAprotect cell reagent (Qiagen, Les Ulis, France). This procedure was previously validated in the coordinating center. EUS-FNA-W were centrifuged at 8 000 rpm Cell pellets were immediately frozen at – 80°C until nucleic acid extraction. Extraction of nucleic acids was centralized in a single platform of molecular biology at the IUCT Oncopole of Toulouse. DNA and mRNA  were extracted using AllPrep DNA/RNA/miRNA Universal kit (Qiagen, Les Ulis, France) according the manufacturer's protocol 150 ng of total RNA was used to generate RNA-seq sequencing libraries using Lexogen Quantseq 3' FWD kit and sequenced by the Institut du cerveau (Paris, France) iGenseq platform on a NovaSeq 6000 apparatus aiming for a minimum of 10 million reads.