title: GSM7350728: E10.5 Msx1+ mesenchymal progenitors, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: About 20 hindlimb buds were dissected from E10.5 Msx1-P2A-tdTomato mice. These limb buds were dissociated into single cells first and resuspended in PBS with 1% BSA. The sorted Msx1+ cells were both counted and adjusted to the concentration of about 1 × 10^6/ml. Then the suspension was centrifuged at 550g for 5 min at 4°C and repeated twice. Cells were counted and cell viability was confirmed by Countess II Automated Cell Counter (Thermo Fisher, Cat# AMQAX1000). Samples were then used for single-cell RNA sequencing (scRNA-seq) with the 10× Genomics system (Library preparation and sequencing were performed by Berry Genomics Inc, Beijing).