title: Cascade evolution in poxviruses: retrotransposon-mediated host gene capture, complementation and recombination
design: Cascade evolution in poxviruses: retrotransposon-mediated host gene capture, complementation and recombination
library_construction_protocol: Virus particles were partially purified from lysates by sucrose gradient centrifugation.  RK13 cells were maintained in DMEM + 5% FBS + gentamicin. 48 hours prior to infection, RK13 cells were seeded into 6 well plates at (500000 cells/well). Confluent RK13 cells were infected at MOI = 0.2 (2.40E+05 pfu) Viral genomic DNA was isolated using standard published methods. PacBio CCS