title: 16S rRNA gene sequences of sludge: inoculum
design: DNA was extracted from the sample using the FastDNA  SPIN Kit for Soil kit.. The V4 region of microbial 16S rRNA gene sequences was amplified by the polymerase chain reaction (PCR) using primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3).