title: GSM8212626: PC-3 (prostate cancer) cells, L-pampo™ 1x, 3 hours, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: The cells were washed twice using 1x DPBS (WelGENE) to extract the total RNA. After removing all the DPBS, 1ml of TRIzol (Invitrogen, Waltham, MA, USA) was added to each well. The TRIzol/cell lysate was collected in a 1.5 mL EP tube after briefly scraping each well and incubated at room temperature for 5 min. Then, 250 µl chloroform was added into the EP tube and shaken vigorously ten times. The EP tube was centrifuged at 14,000 rpm at 4°C for 5 min. The cleared supernatant was transferred to a new EP tube, and 500 µl of isopropanol was added, mixing it carefully. The EP tube was centrifuged at 14,000 rpm at 4°C for 20 min. The isopropanol was discarded, and the pellets were allowed to air dry. Libraries were generated using the Illumina TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA) according to the manufacturer's protocol.