title: GSM8209060: FcgRIIB-/- scRNA 2; Mus musculus; RNA-Seq
library_construction_protocol: Murine spleens processing the tissue into a single cell suspension followed by RBC lysis using Tris-Ammonium Chloride. CD3-B220+CD19+IgD- and CD3-B220+CD19+IgD+ cells were sorted from each animal and mixed at a ratio of 9:1 (IgD-: IgD+) prior to library preparation. Libraries were prepared using the 10x Genomics Chromium Single Cell 3' Reagent kit v2 per manufacturer's instructions.