title: GSM6869031: C24×Col_replicate3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Trizol(Invitrogen, CA, USA). RNA purity and integrity was monitored by NanoDrop 2000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and a Bioanalyzer 2100 system (Agilent Technologies, CA, USA). RNA contamination was assessed by 1.5% agarose gel. Poly-A RNASeq library preparation and high-throughput sequencing were performed by Wuhan Frasergen Bioinformatics Co. Ltd and we used the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA) to generate sequencing libraries, following the manufacturer's recommendations. Libraries were sequenced based on Novaseq 6000 guidelines (Illumina). Three biological replicates were performed for RNA-seq.