title: GSM7162633: RNAseq of control gastruloid at 48h replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Gastruloids for each condition were collected and pooled in a 1.7 ml Eppendorf tube. After centrifugation and medium removal, pelleted stembryos were washed once in PBS, pelleted, snapfroze in liquid nitrogen and stored at -80°C until RNA extraction. RNeasy Plus mini kit (Qiagen) with on-column DNase digestion was used for RNA extraction following the manufacturer's instructions. We performed a "Illumina stranded mRNA ligation" (ISML) prep starting from 1000ng of RNA (see table below), according to Illumina protocol 1000000124518 v01 (internal exp code LPX362 and LPX364). We quantified the libraries by qubit DNA HS and performed profile analysis by TapeStation TS4200. All libraries look good and have sufficient yields.