title: GSM7865484: iAT1 24hr; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were dissociated to single cell using 0.05% trypsin for 3D samples. Each were sorted for live cells using Calcein blue. Single cells were captured for sequencing library preparation using a 10X Chromium (10X Genomics, Pleasanton, CA) instrument at the BUMC Single Cell Core. Library preperation was done on separate days for each sample, but sequencing was done together. Single-cell RNA-seq libraries were prepared according to the Single Cell 3' v2 Reagent Kits User Guide (10X Genomics). Sequence libraries were constructed using the Chromium Single-Cell 3' Library Kit (10X Genomics). Sequencing libraries were loaded on a NextSeq500 (Illumina) to obtain a sequencing depth of~50K reads per cell. 685 cells from CKDCI 3D, 1340 cells from LDCI 3D, 643 cells from LDCI ALI p0, and 1093 cells from LDCI ALI p1 were captured and sequenced.