title: GSM6924918: WT-3 ACE-seq; Mus musculus; OTHER
library_construction_protocol: The ACE-seq libraries were constructed following the published protocol (Yan, et al., Nature Genetics, 2022), and 200 full-grown GV oocytes were pooled as one biological replicate. In brief, gDNA was first fragmented by using the Covaris M220. Then, UDP-glucose and T4 Phage β-glucosyltransferase (New England Biolabs) were used to modify 5hmC to 5ghmC at 37°C for 1 h. After that, 0.1 N NaOH was added to the modified fragments for denaturation at 55°C for 10 mins. Then, the APOBEC enzyme (New England Biolabs) was used to deaminate C/5mC to U at 37°C for 3 hrs. After deamination, DNA fragments were purified and subjected to the library construction. Finally, the ACE-seq libraries were checked and sequenced on an Illumina Hiseq X-Ten platform (Novogene) by 150 bp paired-end sequencing.