title: GSM8122855: E11.5 trachea, Mutant Dermo1Cre; Wnt5af/f, rep.9; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using RLT lysis buffer according to manufacturer's instructions Libraries were generated from Ultra-Low Input mRNA (eukaryotic) Poly(A) selection with enrichment for full-length transcripts. Reverse transcription and cDNA amplification were performed with the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech). Libraries were constructed using the Illumina Nextera™ XT kit.