title: GSM6500497: KL-18, KO, Female, RoomAir; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from frozen lung samples was isolated using the Zymo micro kit  (Zymo Research, Irvine, CA). Sample concentration was assayed using Nanodrop-8000 (Thermo Scientific, Wilmington, DE), and quality checks were done using the NanoDrop spectrophotometer and the Agilent Bioanalyzer. RNA QC was done using AATI or Agilent 2100. Libraries were created using the NEBNext Ultra™ II RNA Library Prep Kit. Briefly, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.