title: rna-seq
design: The extraction of total RNA was carried out using Total RNA Isolation Kit. Subsequently, sequencing was performed on Illumina HiSeq 2500 machines. The raw reads obtained were aligned to the reference genome (version mm10) employing hisat2. To generate the count table of genes, HT-Seq was employed with default parameters. Differential gene expression analysis was conducted using DESeq2 with its default parameters.