title: GSM8163887: Prostate, Organoid, NPK, 1; Mus musculus; OTHER
library_construction_protocol: RNA was extracted from snap-frozen tissues and organoids by homogenization in Tripure Isolation Reagent (Sigma). Only RNA samples having between >200 ng and 1000 ng and with an RNA integrity number >6 were used. Samples were sent to Alithea Genomics SA (Lausanne, Switzerland) for library preparation and sequencing using highly (prostate tissue) or extraction-free (organoids) multiplexed 3'-end bulk RNA barcoding and sequencing (MERCURIUSTM BRB-seq service 50). The generation of bulk RNA Barcoding and sequencing (BRB-seq) libraries was performed using the MERCURIUSTM BRB-seq (prostate tissue) or DRUG-seq (organoids) library preparation kit for Illumina and following the manufacturer's manual (Alithea Genomics, #10813 (prostate tissue); #10841 (organoids)). All libraries were sequenced on Illumina Novaseq 6000.