title: GSM6661550: r69: Rds, LV-IG treated as control, biol rep 3; Mus musculus; RNA-Seq
library_construction_protocol: The RNeasy RNA extraction kit was used to isolate total RNA from dissected neural retinas without the retinal pigment epithelium and other ocular tissues.  On-column DNaseI digestion was performed and RNA was collected in RNase-free water, following manufacturer's instructions (Qiagen, Hilden, Germany).  The RNA quality was determined on an Agilent 2100 Bioanalyzer.   All samples passed the quality filters. Sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold Library Prep kit.  Libraries were loaded onto the HiSeq 4000 Sequencer for paired-end sequencing with read length of 69 bp.