title: EM-seq methylation data from o287561_08-D8P
design: Sample collection from one of three sites. Two treatment types: polluted or non-polluted from Lake Maggiore, Italy.Extraction using DNeasyBlood and Tissue DNA extraction kit (Qiagen). Enzymatic technique to convert unmethylated cytosines in thymidine; NEB Next Enzymatic Methyl-seq (New England Biolabs). DNA extraction before shearing. For each individual DNA extraction, DNA was sheared with a Qsonica sonicator (Q800R2 instrument) using different shearing times depending on the level of DNA integrity. Final libraries were checked on a TapeStation 4150 Instrument (Agilent Technologies). For pooled sequences, we used equimolar amounts of individually extracted DNA. Reads were quality-assessed using FastQC v.0.11.9 and MultiQC v.1.9.