title: Multi-tissue transcriptome analysis for the identification of candidate genes associated with weight regulation in Hanwoo cattle
design: Multi-tissue transcriptome analysis for the identification of candidate genes associated with weight regulation in Hanwoo cattle
library_construction_protocol: To identify expression quantitative trait loci (eQTL) in Hanwoo cattle, 32 animals were provided by the Hanwoo cattle Research institute, National Institute of Animal Science, South Korea. For RNA-sequencing, tissue samples, such as Abdominal Fat (ABF), Back Fat (BFT) and Kidney Fat (KIF)   were collected from the castrated Hanwoo cattle after slaughtering at 10~26   months of age.  Ethics approval was obtained from the National Institute of Animal Science (approval no: NIAS20201979). Total RNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. RNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).