title: GSM8035697: spleen_tumor_d7_R1,GEX; Mus musculus; RNA-Seq
library_construction_protocol: Sorted T cells were either processed for immediate scRNA-Seq ("d0") or transferred into B16KVP tumor-bearing B6 mice and re-isolated by flow cytometry on d7. For multiplexing of d0 samples, biological replicates were marked using hashtags 1-3 and 4-6 for MitoPositive and MitoNegative cells, respectively. Transferred T cells were isolated from spleen (#1 and #2) and tumor (#3 and #4 for MitoPositive and MitoNegative T cells, respectively) and pooled by donor after mulitplexing. Chromium Next GEM Single Cell 3' Reagent Kits v3.1 Droplet sequencing with beads containing polydT capture sequences