title: GSM8035620: SMC2-AID_CDK1as_subclone_1 (biol rep2)_CENP-A_ChIP; Gallus gallus; ChIP-Seq
library_construction_protocol: A nuclear fraction of cells was digested with MNase and solubulised with 0.5M NaCl. CENP-A containing nucleosomes were immunoprecipitated with custom-made CENP-A antibody. ChIP-seq libraries were prepared either using NEBNext Ultra II prep (WT, SMC2, SMC3) or KAPA Hyper prep (CAP-H, CAP-H2).