title: GSM8209362: Pancreas, KCIL17-Rafl/fl, replicate4; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions were prepared by mincing murine pancreas followed by digestion with Collagenase P (4 mg/mL), Dispase (4mg/mL) and DNAse (0.5 mg/mL) and incubation for 5-10 minutes at 37°C in a shaker. Samples were then filtered through a 70-μm and 40-μm strainer and stained with L/D aqua (Thermo Fisher) at Room temperature for 10 minutes. Samples were resuspended in PBS with 0.004% bovine serum albumin and sorted for live cells using Aria II sorter. Cell viability was measured using Trypan Blue. Single-cell suspensions were loaded in a 10x Genomics (Pleasanton, CA) Chromium instrument to generate gel beads in emulsion. The raw data was processed using cell ranger count (Cell Ranger v2.1.1; 10x Genomics) based on the mm10 mouse reference genome