title: GSM6500359: PBS-treated Mouse 5 NMUR1+ CD4 sample; Mus musculus; RNA-Seq
library_construction_protocol: Nmur1-eGFP + ILC2s, Nmur1-eGFP + CD4 + T cells and Nmur1-eGFP – CD4 + T cells were purified by fluorescence-activated cell sorting (ILC2: CD45 + Lin – CD90.2 + CD127 + KLRG1 + , CD4 + T cells: CD45 + CD3 + CD5 + CD4 + ) from Nmur1 iCre-eGFP mice exposed to 3% DSS. RNA was extracted using the RNeasy Micro Kit (Qiagen). RNA libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs).