title: GSM6923104: Pericytes 7 days infarction n=2 (2 hearts); Mus musculus; RNA-Seq
library_construction_protocol: Mouse cardiac interstitial cells from healthy (control) or infarcted (7 days post-infarction) 8-10 week old mice heart were obtained by enzymatic digestion. Erythrocytes were removed using RBC lysis buffer. NG2 lineage cells, Tomato+/CD31-/CD45- cardiac pericytes were FACS-sorted in 1X PBS, 2% FBS. Single cell RNA-seq libraries were constructed using the Chromium System from 10X Genomics. Single Cell 3' Reagent Kit v3.1 (Dual index) was employed for all the samples according to the manufacter's instructions.