title: GSM8034809: HCT116 cells, treatment, replicate 2; Homo sapiens; OTHER
library_construction_protocol: The remaining surviving cells were harvested, and their genomic DNA was isolated and amplified by 2-step PCR using NEBNext Ultra II Q5 Master Mix (M0544L, New England Biolabs, Ipswich, MA, USA) . The PCR products containing the sgRNA sequences were extracted from the gel, quantified, sequenced, and analyzed  by illumina xten platform. Genomic DNA was isolated and amplified by 2-step PCR using NEBNext Ultra II Q5 Master Mix (M0544L, New England Biolabs, Ipswich, MA, USA)