title: RNA-Seq of Hyperolius ocellatus eye
design: Total RNA was extracted from whole eyes using the Promega Total SV RNA Extraction kit (Promega). Tissue was homogenized in the prepared lysis buffer using the Qiagen Tissuelyser (10 minutes at 20 Hz). Messenger RNA library construction was performed using the Kapa HyperPrep mRNA Stranded with RiboErase kit (Roche). Each indexed sample was pooled in equimolar amounts and sequenced with paired end 150 bp reads on a HiSeq4000 at the QB3 Genomics Core at University of California, Berkeley