title: GSM8072915: VitDconB; Mus musculus; RNA-Seq
library_construction_protocol: Trizol based RNA extraction (phenol and guanidinium isothiocyanate solubilization followed by chloroform phase separation) Directional RNA-seq libraries were prepared from 300ng total RNA using the NEBNext Directional Ultra RNA Library Prep Kit for Illumina (New England Biolabs), with initial polyA+ isolation.