title: GSM7755879: SK-MEL-147 cells, shSCR control, 6 days, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit (Qiagen) and the quality of RNA samples was assessed on a 2100 Agilent Bioanalyzer mRNA was extracted from 2 ug of total RNA per sample using NEXTFLEX® Poly(A) Beads 2.0 (Perkin Elmer, Austin, Texas, USA). Libraries were prepared from mRNA samples using NEXTFLEX® Rapid Directional RNA-seq Kit 2.0 (Perkin Elmer, Austin, Texas, USA). Quality of library preparation was assessed on a 2100 Agilent Bioanalyzer