title: WES of H292 cells
design: For whole exosome sequencing (WES), DNA were extracted from cells using commercial kit (TIANGEN Biotech(Beijing)Co., Chinaaccording to the manufacturer's instructions. DNA quality was determined by examining A260/A280 with NanodropTM OneCspectrophotometer (Thermo Fisher Scientific Inc.). Genomic DNA integrity was checked by 0.5% agarose gel electrophoresis. Qualified DNA were finally quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life TechnologiesQ10210). Libraries of DNA were prepared using 50~200 ng DNA, captured by xGenTM Exome Hyb Panel v2 (Integrated DNA Technologies) following the manufacturers instructions. PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 mode.