title: GSM7830930: WT hypoxia No.5; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy-Mini Kit. Extracted RNA was reverse-transcribed using a High-Capacity cDNA Reverse Transcription Kit. The integrity and quantity of total RNA were measured using an Agilent 5400 fragment analyzer. Total RNA obtained from each sample was subjected to a sequencing library construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) and a NEBNext Poly(A) mRNA Magnetic Isolation Module, according to the manufacturer's protocols.