title: Genome Klebsiella pneumonia
design: Low quality reads were removed with Trimmomatic . Genomes including plasmids were assembled with Unicycler contigs with sizes smaller than 1000 base pairs were removed, and quality was assessed with QUAST. Plasmids were reconstructed, classified, and characterized by MOB-suite and PLSDB Acquired antimicrobial resistance genes, virulence factors, insertion sequences and integrons were screened using multiple dedicated servers/tools such as MobileElementFinder, ResFinder, CARD, BacAnt and Staramr