title: Sex differences in skeletal muscle pathology in patients with heart failure and reduced ejection
design: Sex differences in skeletal muscle pathology in patients with heart failure and reduced ejection
library_construction_protocol: Skeletal muscle biopsies from the pectoralis major were collected from each patient, frozen in liquid nitrogen, and stored at -80°C. Unbiased RNA sequencing was performed from these biopsies.  RNA extraction was performed using the trizol-chloroform-isopropanol method following Trizol reagent solution user guide (ThermoFisher Scientific). RNA purification using the RNA clean and concentrate-5 kit (Zymo) was conducted following the kit protocol resulting in high quality RNA in 20 µl of RNase-free water, confirmed using a nanodrop.  cDNA libraries are sequenced using Illumina NovaSeq platforms, which utilise a paired-end 150 bp sequencing strategy.