title: WGS of Oncorhynchus mykiss: historicalEel5
design: We obtained fin clip tissue samples stored at the Smithsonian National Museum of Natural History. We extracted DNA from each sample using the DNeasy 96 tissue protocol (Qiagen, Inc.). We quantified extracts using the Qubit dsDNA HS kit (Invitrogen) and a Qubit 4 fluorometer (Invitrogen). Single stranded libraries were prepared from extracted DNA according to J. D. Kapp, R. E. Green, B. Shapiro, J. Hered. 112, 241249 (2021).