title: GSM8163748: T1-160_OF-1-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were isolated by using MN Nucleospin RNA isolation kit according to manufacturer's instructions. Library preparation and sequencing of parental versus resistant cell RNA samples were performed at BGI (Hong Kong) according to company's library preparation protocol for BGIseq500. Samples were sequenced as single end. Library preparation and RNA sequencing of inhibitor or gRNA treated T1-160 cells were perfomed at University of Oxford (Oxford, UK). Briefly, RNA was DNase I treated,cleaned and concentrated (Zymo RNA Clean and Concentrator, Zymo Research) then enriched for poly(A) mRNA (NEBNext poly(A) mRNA Magnetic Isolation Module, NEB Biosystems, Ipswich, UK). Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep kit (New England Biolabs). RNA quality was assessed using High Sensitivity RNA Screentape and an Agilent 4200 tapestation. Single-indexed and multiplexed samples were run on an Illumina Next Seq 500 sequencer using a NextSeq 500 v2 kit (FC-404–2005; Illumina, Can Diago, CA) for paired-end sequencing.