title: WGBS of s2011A007_Rrox_1
design: Approximately 5.2 g of genomic DNA spiked with 26 ng of lambda DNA was fragmented by sonication to 200-300 bp with Covaris S220 sonicator, followed by end repair and adenylation. Lambda DNA was used as an unmethylated control for calculating the bisulphite conversion rate. Then, the DNA fragments were treated twice with bisulphite using the EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA). After insert size test and accurate quantification of effective concentration of the libraries by qPCR (quantitative polymerase chain reaction) quantification method, the libraries were sequenced on the Illumina Novaseq 6000 platform (Novogene, Beijing, China) and 150 bp paired-end reads were generated.