title: GSM8440171: BABA12_T2_Leaf_WGBS_3; Solanum lycopersicum; Bisulfite-Seq
library_construction_protocol: DNA was extracted from 100 mg of ground frozen leaf and fruit tissue using Qiagen DNeasy Plant Mini Kit (Qiagen) following the manufacturer's instructions and resuspended in 30 μL pure water. All library preparation and sequencing was conducted by Novogene using NovaSeq 6000 PE150. After quality control, positive control DNAs were added into the DNAs which were then fragmented into 200-400 bp using Covaris S220 and bisulfite treated (Accel-NGS Methyl-Seq DNA Library Kit for illumina, Swift). Ligation of methylation sequencing adapters, size selection and PCR amplification was then performed before illumina sequencing for 20 million paired reads per sample with 30 x sequencing depth.