title: GSM6500448: A673,KDM6A,R1; Homo sapiens; ChIP-Seq
library_construction_protocol: (ChIP-seq) Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. (RNA-seq) Total RNA was isolated and purified using the RNeasy Mini Kit (Qiagen) following manufacturer's instructions. (ChIP-seq) ChIP-seq libraries were prepared using 2-5 ng of input and ChIP samples and the kit NEBNext Ultra DNA Library Prep for Illumina (New England Biolabs) following manufacturer's protocol. (RNA-seq) Illumina Stranded Total RNA Prep kit (Illumina) was used according to manufacturer's instructions.