title: GSM8035243: UUATAC-seq datasets of Gecko_Pancreas; Eublepharis macularius; ATAC-seq
library_construction_protocol: Whole organs were then dissected in the following order: ovary, liver, fat, pancreas, lung, kidney, small bladder, esophagus, skin, muscles, stomach, large bladder, intestine, cloaca, tail root, tail tip, eyes, tongue, brain, and heart. All samples were quickly transferred into cold DPBS, washed and then manually pulverized using a mortar and pestle within a liquid nitrogen bath. The pulverized tissues were suspended in 1mL of Nuclear Isolation Buffer (NIB) and followed by incubation for 1.5-3 min to isolate the nuclei. The suspension of each tissue was transferred to 15 mL centrifuge tubes pre-filled with 5mL of WB (wash buffer, 0.2% BSA, 3 mM MgCl2 in DPBS), filtered through 40 μm strainers and centrifuged at 650 × g for 5 min at 4°C. We resuspended the nuclei in 1 mL of WB and filtered the suspension through 20 μm size strainers, pelleted at 650 × g for 5 min and resuspended in the appropriate amount of DPBS to give a final concentration of 25,000 nuclei per microlitre. Library preparations were performed with the UUATAC-seq protocol