title: GSM7865613: Xal treated ethanol (VOC-solvent), rep 1; Xanthomonas albilineans; ssRNA-seq
library_construction_protocol: RNA extraction was carried out with SV Total RNA Isolation System (Promega) From 100 ng of RNA, we performed the ribosomal depletion with Tru-Seq Stranded Total RNA With Illumina Ribo-Zero Plus rRNA (Illumina Inc.), following the manufacturer's instructions. The sequencing libraries were prepared with TruSeq® Stranded mRNA Library Prep (Illumina Inc.), following the manufacturer's instructions. The size and quality of the libraries was evaluated using the 2100 Bioanalyzer Instrument (Agilent Technologies) with the 12000 DNA Assay Kit (Agilent Technologies). The libraries were quantified by real-time PCR with the QIAseq Library Quant Assay Kit (Qiagen) grouped in equimolar ratios and subsequently subjected sequencing on the Hiseq 2500 instrument (Illumina Inc.), with paired-end rapid run mode (2 X 100).