title: GSM6579253: P38; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted by Cetyl Trimethyl Ammonium Bromide (CTAB) method. And the extracted RNA was then tested for purity, concentration, and integrity. A number of library construction procedures, including end repair, end addition of A, ligation adapter addition, and fragment security screening, were carried out once mRNA is recovered. It was sequenced on the device following PCR amplification and purification. RNA libraries for RNA-seq were prepared using High Sensitivity DNA assay Kit（Agilent Technologies, 5067-4626） following manufacturer's protocols.