title: GSM7698660: SYN_11452; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Fresh-frozen tissue cores (10x5mm) were retrieved and matched H&E slides reviewed by a specialist pathologist to identify areas of tumour. Regions of high tumour content were microdissected from the core and DNA extracted using the Qiagen AllPrep kit (Qiagen). Whole genome bisulphite sequencing libraries were prepared for prostate cancer tissue samples using the CEGX TrueMethyl Whole-Genome kit (v3.1) (Cambridge Epigenetics, UK) with minor improvements as described by Nair and colleagues [PMID: 29807544]. Libraries were run on the HiSeq X Ten with one sample per lane.