title: GSM8122770: Microglia 1 ATAC-seq; Mus musculus; ATAC-seq
library_construction_protocol: The ATAC-seq protocol was done mostly as described previously. Briefly, 5 x 104 cells were washed in 50μl cold PBS, spun down at 500 x g and 4°C for 5 mins, and resuspended in 50μl cold lysis buffer.Resuspended cells were immediately spun at 500 x g and 4°C for 10mins, and the supernatant was remvoed. DNA was extracted using Nextera Tn5 transposase kit. PCR amplification of tagmented DNA was done using the NEBNext 2X mastermix.