title: GSM6720682: cDC1 with nociceptors capsaicin biol rep 2; Mus musculus; RNA-Seq
library_construction_protocol: A thousand cells of each population were sorted directly into 5ul of lysis buffer (TCL Buffer (Qiagen) with 1% b‑mercaptoethanol) Total RNA was captured and purified on RNAClean XP beads (Beckman Coulter). Polyadenylated mRNA was then selected using an anchored oligo(dT) primer (5′‑AAGCAGTGGTATCAACGCAGAGTACT30VN‑3′) and converted to cDNA via reverse transcription.