title: GSM8016953: HMCB cells, siNC, biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen) For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purificationThe library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.