title: Illumina MiSeq paired end sequencing
design: V5-V7 16S rRNA using primers 799F and 1193R to exclude chloroplast reads
library_construction_protocol: DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V5-V7 hypervariable regions of bacterial and archaeal 16S rRNA genes, and to attach MiSeq adapter and barcode sequences.