title: RNA-Seq of Poly I:C stimulated mussel: Hemocytes
design: TruSeq Stranded mRNA LT Sample Preparation Kit from Illumina was used for RNA sequencing, according to the manufacturers instructions. A cDNA library was prepared from mRNA via reverse transcription. The amount of cDNA in each library was quantified through spectrofluorometric analysis using the Qubit system (Thermo Fisher Scientific, Massachusetts, United States). The paired-end 150 bp reads were obtained by Illumina NovaSeq 6000 sequencer