title: GSM7159317: Y6M_3; Bos grunniens; OTHER
library_construction_protocol: The total RNA was isolated and purified using the TRIzol reagent . The amount and purity of the RNA samples were quantified using the NanoDrop ND-1000. The qualified RNA samples were constructed into a cDNA library, and oligo-dT magnetic beads were used to specifically capture mRNA with polyadenylate by two rounds of purification. A magnesium ion interruption kit was used for fragmentation of captured mRNA at 86°C for 7 min. The fragmented mRNA was pre-mixed with the immunomagnetic beads Dynabeads Antibody Coupling Kit  and m6A Antibody  in immunoprecipitation (IP) buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630) before conducting IP. The IP product was passed through reverse transcriptase . Then E. coli DNA polymerase I was combined with RNase H and mixed with dUTP solution for two-strand synthesis and RNA degradation. AMPure XP beads were used to select the fragment size, and a 300 ± 50 bp library was obtained. Finally, two-terminal sequencing was performed on the Illumina NovaSeq 6000 platform with sequencing mode PE150