title: GSM6756754: RS4.11 AZD1775(Wee1i) scATAseq; Homo sapiens; ATAC-seq
library_construction_protocol: Excess dead cells were depleted from samples using bead-based Dead Cell Removal Kit (130-090-101, MACS miltenyi Biotech). Nuclei were then isolated with 10x Genomics Nuclei Isolation protocol for Single Cell ATAC Sequencing user guide CG000169 Rev D. Nuclei were mixed in 1:1 ratio with mouse nuclei (unrelated to this experiment) and loaded to the Chromium controller. scATAC-seq was performed using Chromium Single Cell ATAC Library & Gel Bead and Chip E Single Cell ATAC Kit (10x Genomics), user guide CG000168 Rev C. Chromium Single Cell ATAC Library