title: illumina sequence data: gut microbiota of healthy & diseased Nile tilapia
design: DNA was isolated from gut samples using Maxwell 16 LEV Blood DNA Kit and The guidelines of 16S Metagenomics Sequencing Library Preparation (https://support.illumina.com/downloads/16s_metagenomic_sequencing_library_preparation.html.) was followed for the steps of library preparation, from genomic DNA to sequencing-ready libraries. The V3V4 region of 16S rRNA gene was amplified (550 bp) using barcoded universal primers (515F/806R).