title: GSM6500439: 16271_LF; Macaca mulatta; RNA-Seq
library_construction_protocol: For cell capture and cDNA  synthesis, single cells were suspended in PBS containing 0.04%  bovine serum albumin. About 8000 cells were added to each  channel, and it is estimated that about 2200 target cells will be  recovered. The captured cells were lysed and the released RNA  was barcoded in a single GEM by reverse transcription. Reverse  transcription was performed on the S1000TM Touch Thermal  Cycler (Bio Rad) at 53 °C for 45 min, then at 85 °C for 5 min and  held at 4 °C. The quality of cDNA was evaluated using Agilent 4200  (CapitalBio Technology, Beijing). Single-cell suspensions were made using a gentle MACS Octo (Miltenyi Biotec). Single-cell suspensions were loaded onto the Chromium Controller (10× Genomics) for droplet formation. scRNA-seq libraries were constructed by the Single Cell 3' Library and Gel Bead Kit V3. The libraries were sequenced by an Illumina Novaseq6000 sequencer, the sequencing depth of each cell was at least 100,000 reads, and the pairedend 150 bp (PE150) reading strategy (CapitalBio Technology, Beijing) was adopted.