title: GSM8017816: cdk2; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was harvested from the survived cells after the screen maintained coverage of >500 cells expressing each sgRNA used the Zymo Research Quick-DNA Midiprep plus kit (Zymo Research, #FD4075) according to the manufacturer's protocol. The extracted genomic DNA was used for PCR using NEBNext High Fidelity 2 X PCR Master Mix (NEB, #M0541S). The NGS primers that amplify the sgRNA library were listed in the protocol.