title: GSM7645446: CC-seq, 6h, biol rep 1; Lachancea kluyveri; OTHER
library_construction_protocol: 3 x 10^10 cells were harvested for each CC-seq experiment. Cells were lyzed in 2 ml screw cap tubes with 300 microliters of glass beads (425-600 micrometer of diameter, Sigma) per tube using a Fast prep (MP Biomedicals). We used three cycles of 30 seconds at 4 m/s, with five minutes on ice between the cycles. Cell lysates were harvested by centrifuging the screw cap tubes after drilling a hole at their base for 2 minutes at 950g at 4°C. From this point, cell lysates were treated as in Gittens et al. 2019. As in Gittens et al. 2019