title: GSM6866084: immune cells, no tumor, scRNAseq [S0096_Praj_HGG]; Mus musculus; RNA-Seq
library_construction_protocol: Tumor or similar-sized normal tissue was excised from the brain's right hemisphere, where the cells had been injected. The tissue was minced, triturated, and digested with 20 U of Papain for 20 minutes at 37°C. After digested tissue was triturated and passed through a 70 µm cell strainer, CD45+ cells were isolated with a Miltenyi positive selection kit, according to the manufacturer's instructions. Cells were run on a Chromium controller (10x Genomics). Sequencing results were mapped to the mm10 mouse genome reference build and quantified using the CellRanger v3.0.2 (10xGenomics).