title: GSM6435802: D3.5_1_XAV_NANOGNeg; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were dissociated with acutase and washed twice in wash buffer (0.04% Bovine Serum Albumin in Ca2+/Mg2+-free PBS), filtered using 40um strainer and counted on the Countess II automated cell counter (Thermo Fisher). Library construction by Truseq; 8bp dual indices RNA seq libraries were prepared by Truseq and seqeunced on HiSeq4000, 2x150bp, paired-end