title: GSM7622793: huIFNAR mouse, mock, 15 weeks, 3 mice pooled, rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Intrahepatic lymphocyte cell isolation was done by enzymatic digestion. Briefly, the liver was minced into 0.5-mm3 pieces in digestion buffer (RMPI 1640 + 5%FBS + 20ng/ml DNase I + 0.5mg/mL Collagenase D), and enzymatically digested with 37C_m_LIDK_1 by gentleMACS. Liver homogenates were passed through a 70-µm cell strainer and then centrifuged at 300rpm for 5 min. Resuspend the pellet in 5 ml of 40% Percoll and centrifuge at 2000rpm for 20 min without brake. After the supernatant was removed, the pelleted cells were suspended in RBS lysis buffer and incubated on ice for 5 min to lyse red blood cells. After washing twice with PBS, the cell pellets were re-suspended in PBS (containing 0.04%BSA). DNBelab C Series High-throughput Single-Cell RNA Library (MGI, #940-000047-00) was utilized for scRNA-seq library preparation.