title: GSM6924995: WT-6 RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Ten GV oocytes in each biological replicate were pooled, lysed and subjected to first-strand cDNA synthesis. cDNA was purified once by using the Ampure XP beads. Next, purified cDNA products were fragmented by using the Covaris M220, and an RNA-seq library was prepared by following the instruction manual of the NEBNext UltraII DNA Library Prep Kit for Illumina (New England Biolabs). Finally, the libraries were checked, pooled and sequenced with 150-bp paired-end reads on the Illumina HiSeq X-Ten platform (Novogene).  Batch1: WT-1, WT-2, WT-3, KO-1, KO-2, KO-3; Batch2: WT-4, KO-4, KO-5, HD-1, HD-2, HD-3; Batch3: WT-5, WT-6, Tet3KO-1, Tet3KO-2, D3aKO-1, D3aKO-2, DKO-1, DKO-2, KO-6, KO-7.