title: competitive growth assay Tf2null vs WT, h+ and h-, CoCl2 0uM and 250uM, exponential growth, Schizosaccharomyces pombe, T0
design: Purified the genomic DNA from each timepoint with the Monarch gDNA extraction Kit (New England Biolabs). We then amplified the region of one of the mutations acquired by the Tf-null strain (I:1453108 C->A) with primers amenable to Amplicon-seq with in-line barcodes to identify the samples in pooled sequencing runs (oM2327-oM2339), purified the PCR products, measured their concentrations and pooled them in groups of 12 for Amplicon-Seq (Genewiz, Piscataway, NJ).