title: GSM7111382: Uninfected; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted by flow cytometry into a low retention 1.5 mL collection tubes, containing 500 μL of a solution of PBS:FBS (1:1) supplemented with RNase inhibitor (1:100). After sorting, ice-cold PBS was added to make up to a volume of 1400 μl, spun down and single-cell libraries prepared as per the manufacturer's instructions (10x Genomics). Samples were processed using 10X v2 chemistry as per the manufacturer's recommendations; 11 and 12 cycles were used for cDNA amplification and library preparation, respectively. Single-cell libraries prepared as per the manufacturer's instructions (10x Genomics). Samples were processed using 10X v3 chemistry, as per the manufacturer's recommendations.