title: GSM6923358: EMS EXO 02; Homo sapiens; miRNA-Seq
library_construction_protocol: Use the 200ng-1μg of RNA sample，then separate RNA segment of different size by PAGE gel, select 18-30nt(14-30 ssRNA Ladder Marker，TAKARA)stripe and recycle; Adaptor ligation:Prepare connection 3'adaptor system (Reaction condition:70°C for 2min; 25°C for 2h);Secondly add RT-Primer, (Reaction condition: 65°C for 15min;ramp to 4°C at a rate of 0.3°C/sec);Thirdly add 5'adaptor mix system(Reaction condition:70°C for 2min; 25°C for 1h);  RT -PCR:Prepare First Strand Master Mix and Super Script II (Invitrogen) reverse transcription (Reaction condition:42°C for 1h;70°C for 15min);Several rounds of PCR amplification with PCR Primer Cocktail and PCR Master Mix were performed to enrich the cDNA fragments (Reaction condition:95°C for 3min;15-18 cycles of(98°C for 20s，56°C for 15s，72°C for 15s);72°C for 10min;4°