title: GSM8439440: MIX treatment, biological replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Control or cytokine treated THP-1 macrophages were first washed with PBS, then trypsinized for 10 min at 37°C (Gibco, cat. 25200). Trypsinization was halted by the addition of an equal volume of warm RPMI medium with 10mM HEPES and 10% FBS, cells were spun at 400×g for 5 min and resuspended in 1.0% PBS/bovine serum albumin (BSA). Cells were filtered through 40μm cell strainer to exclude cell clumps. Gel bead-in-emulsion (GEM) encapsulation and single cell indexing reactions were performed using a Chromium X™ Controller instrument (10× Genomics, Inc., Cat. No. 1000202). Single-cell 3' RNA-seq libraries were prepared using the Chromium Next GEM Single Cell 3' GEM, Library and Gel Bead Kit (version 3.1 chemistry, 10× Genomics, Inc., Cat. No. 1000121), according to the manufacturer's instructions.