title: GSM8440079: R1-50,scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: The 10X Genomics 3 'transcriptome utilizes short read length sequencing and microfluidic technology to achieve simultaneous transcriptome expression profiling analysis of 500-10000 cells per sample. We combine gel beads containing barcode information with the mixture of cells and enzymes, and then wrap them with oil surfactant droplets located in the microfluidic "double cross" system to form GEMs (GelBeads In Emulations). GEMs flow into the reservoir and are collected. The gel beads dissolve and release Barcode sequences, reverse transcribe cDNA fragments, and label the samples. The gel beads were broken and the oil drops were broken, and the cDNA was used as the template for PCR amplification. Mix all GEMs products and construct a standard sequencing library. Firstly, the cDNA enzyme is cleaved into fragments of about 200-300 bp, followed by the traditional second-generation sequencing library building process such as sequencing adapter P5 and sequencing primer R1. Finally, PCR amplification is performed to obtain the DNA library.