title: GSM7794629: HUVECs treatment with control [CK1]; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA was harvested using Trizol reagent. A total of 2 µg total RNA per sample was used as input material for the cirRNA library preparation. ribosomal RNA is removed and rRNA residues are removed by ethanol precipitation. Subsequently, linear RNA was digested with 3 U of RNase R (Epicentre, USA) per µg of RNA.Sequencing library by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) is produced as recommended by the manufacturer Agreed.recommendations and index codes were added to attribute sequences to each sample