title: GSM8212436: DP009; Homo sapiens; RNA-Seq
library_construction_protocol: Blood samples were kept in PAXgene RNA tubes in -80 until ready to process. RNA was isolated using the PAXgene Blood RNA kit according to the manufacturers guidelines (PreAnalytiX). Eluted RNA was dissolved in RNase-free water. The quality and quantity of RNA were evaluated using the Agilent 2100 BioAnalyzer (Santa Clara, CA, USA). Three hundred ng of total RNA Total was ribodepleted using the RiboCop Depletion Kit Human/Mouse/Rat v2 (Lexogen Inc., Greenland, NH).  Stranded RNA-Seq library construction was performed using the xGen Broad-Range RNA Library Prep Kit (Integrated DNA Technologies, Coralville, IA).  Library concentration was measured with a Qubit fluorometer (ThermoFisher Scientific), and library size was evaluated on a 4200 TapeStation (Agilent Technologies). Multiplexed libraries were sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) using 75bp single-end sequencing. On average, approximately 50 million reads were generated from each sample.