title: GSM7783775: DM-8; Homo sapiens; miRNA-Seq
library_construction_protocol: Total Exos RNA was isolated by miRCURY RNA Isolation Kit–Cell & Plant (Exiqon, Woburn, MA) according to the manufacturer's instructions. RNA integrity was analyzed on the 2100 Bioanalyzer using the RNA 6000 Pico Kit (Agilent Technologies, Santa Clara, CA) and RNA was quantified using the Qubit RNA HS Assay Kit (ThermoFisher Scientific). The QIASeqTM miRNA Library Kit (Qiagen, Hilden, Germany) was used to prepare miRNA sequencing libraries. Final library concentrations were measured using the Qubit 1X dsDNA HS Assay kit. Library fragment size was determined on the 4200 TapeStation (Agilent Technologies). Libraries were sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) at an average sequencing depth of ~10M reads/sample and 1x75 bp sequencing.