title: GSM6435867: young_spleen_1, replicate 1, scRNAseq [TCR]; Mus musculus; RNA-Seq
library_construction_protocol: Samples were prepared for single-cell 5′ Gene expression and VDJ repertoire immune profiling, using the Chromium Single-Cell Controller (10X Genomics, CA). A part of the isolated cDNA was set aside for BCR target enrichment and VDJ repertoire library preparation, while another of the cDNA was processed for 5′ gene expression (GEX) library. Each library was sequenced on an Illumina NovaSeq-6000 platform to generate 151x151 paired-end reads. Processing of raw sequencing reads for each library was carried out using the Cell Ranger pipeline (v 6.0.1, 10x Genomics, CA).