title: Oxford Nanopore genomic sequencing of Hyptiotes cavatus: adult female cribellar gland
design: Genome sequencing was carried out using Oxford Nanopore PromethION sequence data and Illumina reads for error-correction. DNA was extracted from a combined sample of silk glands from 10 individuals using the Gentra Puregene tissue kit (Qiagen cat 158667) and treated with the Circulomics short read eliminator kit (PacBio cat SS-100-101-01). This DNA prep was used for both the Nanopore and Illumina sequencing. The Nanopore sequencing was conducted on two PromethION flowcells,