title: GSM7698746: NHDF cells,0.06% Osmoter treatment, biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA were extracted using Trisol reagent After extraction of total RNA from the sample and digestion of DNA with DNase I, eukaryotic mRNA with poly-A tail was enriched by magnetic beads with Oligo (dT), the bound mRNA was eluted, and interrupted by divalent cations and high temperature conditions in interruption buffer. The first strand of cDNA was synthesized by reverse transcription using random primers. The mRNA in the heterozygous duplex was digested by RNase H to synthesize the complementary cDNA strand, and the second cDNA strand was synthesized. After purification, the cDNA was repaired at the end, the base "A" was added to the 3 'end, and the linker was attached. Finally, PCR amplification was performed