title: RNA-Seq of Hevea brasiliensis: leaf
design: Total RNA was extracted from leaves using TRIzol reagent (Thermo Fisher Scientific, 15596018). RNA quality and purity were evaluated using the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA). Ribosomal RNA (rRNA) was removed from total RNA using the Ribo-Zero Gold rRNA Removal Kit (Illumina, San Diego, USA). For lncRNA and mRNA sequencing, strand-specific libraries (fr-first strand) were constructed following the manufacturer's protocol and sequenced on an Illumina NovaSeq™ 6000 platform (LC Bio, China) with paired-end reads of 2*150 bp (PE150)