title: GSM8213015: Taf12 MUT, OG, 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from E3.5, E6.5, or OG embryos individually using the Zymo kit as previously described. Total RNA was analyzed using the Agilent 2100 Bioanalyzer using the Eukaryote Total RNA Pico assay. RNaseq libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina following manufacturer instructions. The quantity of library was assayed using the Qubit DNA HS assay, and quality was analyzed on Bioanalyzer. Libraries were sequenced on Illumina NextSeq 500 platform using NextSeq 500/550 Mid Output v2.5 kit (150 cycles) with 76 bp paired-end sequencing chemistry. RNAseq library preparation and Illumina Next-Generation Sequencing was performed at Genomics Resource Laboratory (RRID:SCR_017907), Institute for Applied Life Sciences, University of Massachusetts Amherst, MA.