title: RAD-Seq of rattus norvegicus: rat 12
design: DNA was digested using Sbfl restriction enzyme, followed by the fusion of the P1 adapter (containing the barcode) to the resulting fragments. Next, we pooled all individuals and amplified fragments by doing PCR using the forward primer contained in the P1 adapter sequence. PCR products were gel purified by excising a DNA fraction of 350-600 bp