title: GSM8439104: MCF7, ATAC-seq with Illumina TDE1, replicate 2; Homo sapiens; ATAC-seq
library_construction_protocol: Prior to tagmentation, cells were treated in culture medium with DNase (Worthington cat# LS002007) at a final concentration of 200 U/mL at 37 °C for 30 mins, then washed twice with PBS at 500g, RT. For Illumina enzyme tagmented material, 2.5 µL of Illumina TDE1 enzyme was used. K562 transposome titration experiment: pG-Tn5 tagmentation was titrated with the following amounts of loaded transposome: 1.0, 2.5, 5.0, 7.0 µL. Libraries were PCR amplified  with Illumina i5 or i7 barcoded primers, with 7 cycles of PCR were performed during library preparation for the K562 experiment and 8 cycles performed during library preparation for the MCF7 experiment.