title: M treated at -3d and time zero rep2
design: Plants were treated with M as indicated. Plant tissue was collected at indicated time points, 0 h / 24 h / 72 h after B. cinerea inoculation of plants treated with M 3 days before inoculation and on the day of inoculation. Tissue was harvested from one plant for each biological replicate, with 3 plants sampled for each treatment. Twenty plants were sampled in total. For RNAseq, total RNA was extracted from tomato leaf samples (200 mg) using the GenElute mammalian total RNA miniprep kit (Sigma-Aldrich, USA) according to the manufacturers protocol. Extracted RNA was treated with DNase (TURBO DNA-free Kit, Ambion Life Technologies, USA) to remove possible genomic DNA traces. RNA yield and purity was measured by Nanodrop (ND-1000 Spectrophotometer, Wilmington, USA) and integrity by running in 1.5% agarose gel electrophoresis. For qRT-PCR validation, total RNA was extracted from the leaves of plants treated as indicated, using Tri-reagent (Sigma-Aldrich) according to the manufacturers instructions.