title: GSM6923191: buccal pouch,coated with DMBA (simple hyperplasia), rep 3; Cricetulus griseus; miRNA-Seq
library_construction_protocol: Gradually extract total RNA from tissues according to the experimental instruction manual in Trizol kit A total of 18–30 nt RNA fragments were excised, purified from a PAGE gel, and ligated with 5' and 3' adaptors using T4RNA ligase. Reverse transcription followed by PCR was used to create cDNA based on the small RNA ligated with 3' and 5' adapters. Subsequently, the amplified cDNA constructs were purified from agarose gel, in preparationfor sequencing analysis using the Illumina HiSeq 2500 Analyzer (Illumina, CA, USA) according to the manufacturer'sinstructions