title: RNA-seq of peripheral blood of healthy control
design: RNA was isolated from PAX tubes. 100 to 250ng of RNA was used as input for the Lexogen QuantSeq 3' mRNA libraries. These libraries were sequenced on a Illumina HiSeq4000 generating single-end 51bp reads. Optical duplicates and adaptors were removed with clumpify and fastx-toolkit, respectively. Next the reads were mapped to the human reference genome with Star 2.6. and gene expression matrices were generated with HTSeq (version 0.10.0)