title: GSM8428055: BT16 cells, DMSO, replicate 2, RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Ribonucleic acid was isolated from treated cells using a QIAGEN miRNAeasy kit Cat#217004 (Qiagen, Valencia, CA, USA). 500ng total RNA was used. Libraries were prepared and sequenced by Genomics and Microarray Core Facility (University of Colorado Denver). Universal Plus™ mRNA-Seq library kit with NuQuant (Tecan) was used. Libraries were sequenced on an Illumina NovaSEQ X. High-quality base calls at Q30R ≥80% were obtained with 40M paired end reads.