title: GSM7111941: MDA-MB-231 3D Spheroids: 5.0 nM ZJ-101 sample, biological replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Spheroids and loose cells were harvested using wide-gauge multichannel pipette by gently resuspending all cells and pooling together to pellet in falcon tubes. Cell pellets were briefly washed with ice-cold PBS prior to total RNA extraction via the Rneasy mini kit (Qiagen) per the manufacturers instructions. Libraries were constructued by Genewiz following poly-A selection from total RNA samples, all RNA content was analyzed on an Agilent TapeStation with RIN > 9.5 for all samples.