title: GSM7865226: RH4 PD circHIPK3 EVEN #2; Homo sapiens; RNA-Seq
library_construction_protocol: 40 million of cells for each biological replicate were pelleted, resuspended in 1 ml of cold PBS supplemented with 0.5 mg/ml of 4'- aminomethyl-4,5',8-trimethylpsoralen (AMT, Cayman Chemical) per 10.000.000 cells and crosslinked at 365 nm for five 2-minute cycles. 1 volume of Guanidinium Hydrochloride 6M per each volume of AMT was added. Lysates were subjected to RNA pulldown with biotynilated probes and streptadivine beads and  RNA was precipitated through phenol/chloroform extraction. RNA library was produced using Stranded Total RNA Prep with Ribo-Zero Plus (Illumina). All samples were sequenced on an Illumina Novaseq 6000 Sequencing system.