title: GSM6579213: B2, spatial transcriptomics; Homo sapiens; OTHER
library_construction_protocol: Samples were frozen embedded on dry ice. Frozen tissues were sectioned (10μm) and attached to ST microarrays (https://www.10xgenomics.com/). On the one hand, slides were dehydrated, stained with HE, and imaged by a 3D HISTECH Pannoramic MIDI FL scanner. On the other hand, slides were permeated and obtained fluorescently labeled cDNA. The cDNA was digested and fragmented to construct the NDA library. And the NDA library was sequenced using the lllumina novaseq 6000 system.