title: GSM8440351: NCI-H660, Input; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were processed for ChIPseq following published protocol (PMID: 20680851) with the following antibodies: FOXA2 (5µg, R&D #AF2400-SP. 10 million cells were fixed with 1% formaldehyde for 10 minutes at room temperature. Cells were collected. Chromatin was sonicated to approximately 500bp. For ChIP-seq, sequencing libraries (100 ng DNA per sample) were constructed using the KAPA library Prep Kit (Roche, Cat KK8510) with Illumina TruSeq indexes. Libraries were assessed for quality using gel electrophoresis and libraries passing quality control (e.g., no primer dimers) were quantified using the KAPA Library Quantification Kit for Illumina (Roche, Cat KK4824). Libraries were sequenced using the Illumina NextSeq 500 (75 bp single-end reads; cell lines).