title: RNA-Seq of waterlogging stress Rye
design: Specific rRNA probes were used to remove rRNA from Total RNA, The remaining RNA was randomly fragmented in Fragmentation Buffer. Using RNA as the template, the first strand of cDNA was synthesized by random primers, then the RNase H was used to degrade the RNA strand, and dNTPs were used as raw material to synthesize the second strand of cDNA, then the cDNA was purified. Purified double strands cDNA was processed for end-repairment, A' addition and adapters ligation. cDNA within a certain size range was extracted by AMPure XP beads. he USER enzyme was used to degrade the second cDNA strand which contained U. Finally, the cDNA library was obtained by PCR.