title: GSM7698898: PAO1WT2_run2; Pseudomonas aeruginosa; ssRNA-seq
library_construction_protocol: RNA was isolated from the cultures using the MasterPure Complete DNA and RNA Purification Kit (Immunosource, Belgium) following manufacturer instructions. To remove all the gDNA, two additional rounds of DNase treatment were performed using TURBO DNase (ThermoFisher Scientific) following manufacturer's protocol. RNA was subsequently isolated again using the MasterPure RNA isolation kit and the RNA was dissolved in MQ and stored at -80 °C until further use. Truseq RNA stranded ribo-zero