title: GSM8016900: Muscle, all samples, Rep1-5 each; Nothobranchius furzeri; RNA-Seq
library_construction_protocol: Tissue samples were lysed in TRIzol reagent (Thermo Fisher Science) and extract RNA by using RNA Clean & Concentrator kit (Zymo Research). Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Same tissue samples were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.