title: GSM8072209: H1a; Equus caballus; RNA-Seq
library_construction_protocol: RNA was collected using Tri-reagent (Sigma-Aldrich) and extracted using the RNeasy Mini Kit (Qiagen, UK) following the manufacturer's instructions. Purified RNA was treated with the DNA-free™ DNA removal kit (Invitrogen, Thermo Fisher) to remove genomic DNA contamination according to the manufacturer's instructions. RNA concentration was determined using a Qubit (Thermo Fisher Scientific) and the purity was determined using a DS-11 spectrophotometer to ensure 260/280 ratios >1.8. RNA integrity was measured using a Tapestation (2100 Bioanalyzer (Agilent, UK) and for all samples was confirmed to be >9.0. mRNA library preparation and transcriptome sequencing were conducted by Novogene (Cambridge, UK) using an Illumina NovaSeq 6000 to generate 26.1X-57.2X million 150 bp paired end reads per sample.