title: GSM7111295: ap1tetmut_h24_dex_chip; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: 2.5 to 3 g of seedlings and around 1g inflorescence were collected for ChIP-seq. Plant tissues were fixed in formaldehyde, and ChIP-seq was done according to the published protocol (Kaufmann, K., Muiño, J.M., Østerås, M., Farinelli, L., Krajewski, P., and Angenent, G.C. (2010a). Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP). Nat. Protoc. 5: 457–472.). Nuclei lysate was incubated with native SEP3 antibody for 1 hour, and 50 µl Dynabeads protein A (Thermo Fisher) was then added for immunoprecipitation. Libraries were prepared suing the ThruPlex DNA-Seq kit (TaKaRa).