title: GSM7350388: 48 hr - IFNG; Homo sapiens; RNA-Seq
library_construction_protocol: Right after the treatment period ended, MDA-MB-231 cells were trypsinized and then kept in a suspension mode for cell viability assessment and the subsequent library prep using the 10x Genomics Chromium Single Cell V3  3' capture kit. The library was performed according to the manufacturer's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, MDA-MB-231 cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was reverse-transcripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI), and the 10x Barcode. The pooled barcoded cDNA was then cleaned up with Silane DynaBeads, amplified by PCR, and the appropriated-sized fragments were selected with SPRIselect reagent for subsequent library construction. The amplified and purified cDNA was prepared and loaded to the automation platform SciClone to finish the single-cell library prep for Illumina sequencing.