title: The AP-1 transcription factor Fosl-2 drives cardiac fibrosis and arrhythmias under immunofibrotic conditions
design: The AP-1 transcription factor Fosl-2 drives cardiac fibrosis and arrhythmias under immunofibrotic conditions
library_construction_protocol: Cells were harvested by trypsinization, washed with PBS and mixed in RNA lysis buffer (Zymo Research).  Cardiac tissue was lysed in 600 µL of RNA lysis buffer (Zymo) and homogenized using TissueLyserII (Qiagen). Homogenate was spun down for 2 min at 15000 g and the supernatant was processed according to the manufacturer's instructions. Zymo Quick-RNA MicroPrep isolation kit was used to extract total RNA. 100% ethanol was added to the lysates and samples were than processed on the columns. DNA contamination was removed by DNase I digestion. RNA was washed and eluted in 20 µl of water. RNA concentration and purity were assessed with Nanodrop 1000 (Thermo Fisher Scientific)  RNA-seq library preparation workflow Figure 2.2.1 Strand-specific RNA sequencing with polyA selection.