title: GSM8000549: BT-549 TAZ+IPATA (COMBO) rep1, ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: Suspensions of 50,000 cells with viability > 90% were subjected to nuclei isolation, followed by Tn5-mediated transposition. ATAC-Seq libraries were prepared using the Omni-ATAC-Seq protocol. The Zymo DNA Clean and Concentrator 5 kit was used to clean up the transposed reactions, after which Illumina adapter sequences and barcodes were added to the transposed DNA fragments in a pre-amplification step.  Quantitative PCR was performed to determine the appropriate number of additional PCR amplification cycles, as to not overamplify the reaction.  Amplified libraries were purified using the Zymo DNA Clean and Concentrator 5 kit, followed by a double-sided SPRIselect Bead cleanup (Beckman Coulter, Brea, CA).   Final libraries were analyzed on a 4200 TapeStation (Agilent Technologies, Santa Clara, California) and quantified via quantitative PCR using the Collibri Library Quantification Kit (Thermo Fisher Scientific, Waltham, MA).