title: GSM6870649: Somatic cell samples 21-40; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using Macherey-Nagel NucleoSpin® RNA XS extraction kit (Scientifix, 740902.50). Libraries were prepared with 30ng of RNA. Novel RNA-Seq pipeline. Briefly, index is added during initial pA priming and pooled samples amplified using template switching oligo. P5 is added by PCR and P7 by Nextera transposase. I7 indexes were added to the final libraries to enable parsing of sample sets. Sequencing was performed on NextSeq2000 (Illumina) following Illumina protocol 1000000109376 v3.