title: DNA-Seq of Col-0 sperm nuclei rep3
design: Genomic DNA was extracted using QIAamp DNA Micro Kit (56304) following the provider Small Volumes of Blood'' protocol. DNA was diluted to 10 ng in 50l of elution buffer (10 mM Tris-HCL, pH 8.0) in  microTube AFA Fiber tubes (520052). Samples were sonicated in Covaris E220 programmed to 80s / 10 Duty / 140 PIP / 200 cycles. 25 l of the sonicated DNA was used for library preparation using the NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S), with a final amplification of 12 PCR cycles. Libraries were sequences on Illumina Novaseq or Nextseq machines, with at least 10 million paired-end reads per library.