title: GSM8033678: D6_2i_50uM_R1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with NZY Total RNA Isolation kit (NZYtech #MB13402) and were converted into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq V2 Library Prep Kit (FWD) for Illumina" (Lexogen #191) Library generation was initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. Primers from both steps contained Illumina-compatible sequences. Libraries were completed by PCR and sequenced in Illumina NextSeq 550 according to manufacturer's protocols