title: GSM6923424: ATAC-seq_hAFE_rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Samples were processed according to (PMID 30995470) ]. Briefly, approximately 50,000 cells were pelleted at 750 x g for 15 minutes at 4°C. After the addition of lysis buffer (10 mM Tris-Cl pH 7.4, 10 mM NaCl, 3 mM MgCl2, and 0.1% IGEPAl CA-630), nuclei were pelleted at 750 x g for 15 minutes at 4°C and the supernatant was discarded. Transposase reaction was performed at 37°C for 30 minutes. The purified, tagmented DNA was amplified for 9–10 cycles and size selected using AMPure XP beads (Beckman Coulter, A63881) as follows: 0.55X volume of 2X concentrated AMPure beads was added to the amplified DNA and incubated for 5 minutes at room temperature. The supernatant, containing low-molecular weight DNA, was collected in a separate tube by removing the beads using a magnetic rack. 1X volume of AMPure beads was added to the supernatant and incubated for 5 min at room temperature. The beads were washed twice with 75% ethanol and then air-dried. The final purified library was eluted in EB buffer and sequenced (paired-end, 75–75) on an Illumina NextSeq500 platform.