title: GSM8440357: HFD+MGIG 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer' s instructions. Then RNA quality was determined by 5300 Bioanalyser(Agilent) and quantified using the ND-2000 (NanoDrop Technologies). The RNA-seq transcriptome librariy was prepared following SMART-Seq_V4 Ultra Low Input RNA Kit for Sequencing from Clontech (San Diego, CA) using 10ng of total RNA.