title: GSM8427483: stable THP-1 cell line selected after transfection with an vector expressing ELDR  (Lego-lnc-ELDR) rep2 C1; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq was carried out with 50,000 cells washed once with 50μl of cold 1X PBS buffer. Spin down at 500g for 5 min, 4 °C. Gently pipetted to resuspend the cell pellet in 50μl of cold lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Cells were spin down immediately at 500g for 10 min, 4°C and lysed with non-ionic detergent to enrich nuclei. Pellets of 50,000 nuclei were tagmented for 30 minutes at 37°C (TDE1 Transposase, Illumina) and tagmented DNA was purified on column DNA clean-5 (Zymo Research). Illumina UDP Indexes were integrated by PCR-enrichment (11 cycles) and final libraries were purified and size selected (L=1.1; R=0.6) with ampure beads (KAPA, Roche Diagnotics). Library size distribution was assessed on a 2100 bioanalyzer (Agilent Technologies) and libraries were quantified by qPCR