title: P. pseudocerasus  genomic resequence
design: For Illumina paired-end sequencing, ~1.5g of genomic DNA was used for constructing a 350 bp DNA library using Illumina TruSeq Nano DNA library preparation kit (Illumina, San Diego, CA, USA). The refined libraries were subsequently sequenced using the Illumina Novaseq 6000 platform (Illumina, San Diego, CA, USA), generating 42.76 Gb of raw sequences. The fastp software (v0.23.4)1 was employed to filter out low-quality paired reads. As a result, the remaining 42.68 Gb (99.81%) of high-quality data, with 97.44% and 93.88% of the bases having a quality score of  Q20 and Q30 respectively, was utilized for genome survey and genome assessment analyses