title: Cyanobacterial 16S rDNA from litoral (30-50 cm depth) lake water
design: Water samples were filtered (50  500 mL) through Sterivex filter units  (0.22 m). DNA was extracted using the the DNeasy PowerWater Sterivex Kit from the Sterivex filters. Amplification of  cyanobacterial 16S rDNA V3-V4 region was done using, the primers 515F (GTGCCAGCMGCCGCGGTAA)  CYA781R(a) (GACTACTGGGGTATCTAATCCCATT), CYA781R(b) (GACTACAGGGGTATCTAATCCCTTT), (30 cycles).