title: GSM7162707: 8_UI_1.tabular; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the miRNeasy Micro Kit (Qiagen Cat. no. 217084) per manufacturer's instructions Libraries were prepared at the Yale Center for Genome Analysis. mRNA was purified from 200ng of RNA with oligo-dT beads (Roche Kapa mRNA Hyper Prep Cat# KR1352).  Following first-strand synthesis with random primers, second strand synthesis and A-tailing were performed with dUTP for generating strand-specific sequencing libraries. Adapter ligation with 3' dTMP overhangs were ligated to library insert fragments. Library amplification amplifies fragments carrying the appropriate adapter sequences at both ends. Indexed libraries were quantified by qRT-PCR using a commercially available kit (Roch KAPA Biosystems Cat# KK4854). Insert size distribution was determined using an Agilent Bioanalyzer. Samples with a yield of ≥ 0.5 ng/l and a size distribution of 150-300bp were used for sequencing.