title: GSM6426632: Skin-1; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell RNA-seq analysis was conducted using BD Rhapsody Single-Cell Analysis System (BD BioScience) following the manufacture's protocol. In short, the single cell suspension was loaded into a BD Rhapsody cartridge with >200,000 microwells and single cell capture was achieved by random distribution and gravity precipitation. The bead library was loaded into the microwell cartridge to saturation so that a bead was paired with a cell in a microwell. The cells were lysed in the microwell cartridge to hybridize mRNA molecules onto barcoded capture oligos on the beads. These beads were retrieved from the microwell cartridge into a single tube for the subsequent steps of cDNA synthesis, ExoI digestion and multiplex-PCR based library construction. For library construction, we performed Whole transcriptome analysis alpha protocol (BD Rhapsody).