title: Illumina NovaSeq 6000 paired end sequencing; RNAseq of wild type Col-0 Arabidopsis plants and piezo/feronia/camta3 mutants before and 22 minutes after touch treatment
design: RNAseq of wild type Col-0 Arabidopsis plants and piezo/feronia/camta3 mutants before and 22 minutes after touch treatment
library_construction_protocol: For untreated samples (time point 0 minutes), approximately 12 seedlings were gently lifted from the MS agar plate, placed inside a microcentrifuge tube and snap frozen immediately in liquid nitrogen. Arabidopsis seeds were surface sterilized with chlorine gas. The seeds were sown in plates containing 1x MS with vitamins, 1% sucrose, 1% phytoagar, 0.5 g/L MES and pH adjusted to 5.8. The plates were stratified at 4°C for 3 days before transferring to standard growth condition (21°C, 16-h light/8-h dark cycle, 100 µmol m-2s-1).  Data were processed using Demultiplexing and quality controlled with FastQC.  RNA was extracted using the Sigma Spectrum Plant kit and DNAse treatment. 500 ng of total RNA was used for library preparation using Illumina TruSeq mRNA (poly-A selection) and TruSeq RNA UD Indexes for up to 96 samples (Illumina, 20022371).