title: GSM8000089: female_CTRL3_24h; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using TRI Reagent (Sigma-Aldrich, St Louis, MO, USA). Tissue was homogenized using beads and the Fisher Bead Mill (Fisher, Hanover Park, IL, USA). RNA was further processed on columns using the Direct-zol Kit (Zymo Research, Irvine, CA, USA). Concentration and purity were determined using a BioTek Take3 micro-volume microplate with a BioTek PowerWave XS microplate reader (BioTek Instruments Inc., Winooski, VT). Samples were purified by poly-A enrichment before library creation using Illumina TruSeq Stranded mRNA