title: Fungal sequence: Bulk soil
design: MiSeq sequencing results in double-ended sequence data. First, merge pairs of reads into a sequence according to overlap between PE reads. Meanwhile, quality control filtering is carried out on the quality of reads and the effect of merge. According to the barcode and primer sequences at both ends of the sequence, the samples are differentiated to get the effective sequence, and the direction of the sequence is corrected, which is the optimized data.