title: GSM7698592: d10, hiPSC,BXS0116; Homo sapiens; RNA-Seq
library_construction_protocol: For single cell dissociation, d3, d10, d25 and EHM were treated with digestion medium (Accutase, Trypsin and DNase1) for up to 30 min at room temperature. Cell suspensions were gently dissociated by pipetting, centrifuged (100xg, 10min @RT) and resuspended in PBS. Similarly, hiPSC (d0) were washed with PBS twice and incubated in Versene for 5-10 min, arrested with pre-warmed medium, mechanically dissociated, centrifuged and resuspended in PBS. All cell suspensions were checked for viability (>90%), counted and passaged through a 30 μm mesh to ensure compatibility with the 10x Genomics pipeline. Library was constructed according to manufacter's instructions (Chromium Single Cell 3' Gene Expression Solution v2, 10x Genomics).