title: GSM6661415: IMR32_DMSO_Rep2 (RNAseq); Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed practicing the manufacturer's instructions of miRNeasy kit (Qiagen) including on-column DNase treatment and nanodrop (Thermo Scientific) was used to determine the concentration. RNA quality was determined with the Experion  automated electrophoresis system (BioRad) prior to profiling. 250 ng of RNA-seq  isolated from cell line IMR32 upon 6h time point of CR31B treatment was used as input for library preparation with the TruSeq small RNA library Kit from Illumina. During library preparation 15 PCR cycles were used.  qPCR quantification of the libraries using the illumine Kapa library quantification kit (Lightcycler 480 qPCR mix Kapa). RNA-seq libraries were sequenced on the NextSeq 500 platform (Illumina) using the Nextseq 500 High Output kit V2 75 cycles single-end (Illumina).