title: GSM8427436: LM_SD_7; Homo sapiens; OTHER
library_construction_protocol: New 5 𝜇m tissue sections were cut by a microtome, placed in a 42°C RNase-free water bath and manually transferred onto 6.5 x 6.5 mm tissue capture areas on Visium Spatial Gene Expression Slides (PN-1000185, 10x Genomics). Tissues were deparaffinized, H&E stained, imaged with a Leica Aperio AT2, and de-crosslinked according to the manufacturer's recommended protocol, with the following user modifications made to deparaffinization to prevent tissue detachment: 1) the 15 min incubation step during deparaffinization was removed, 2) after immersion in 96% ethanol, one 85%, one 70% and one 50% ethanol immersion for 3 min each was added. Probe hybridization was performed with the Visium Human Transcriptome Probe Kit (PN-1000364), followed by probe ligation, extension and elution for downstream qPCR cycle determination and 10x library construction with the Visium FFPE Reagent Kit (PN-1000362).