title: GSM8371941: OVCAR8 MYC CUT&RUN Rep2; Homo sapiens; OTHER
library_construction_protocol: CUTANA CUT&RUN kit (EpiCypher) was used on proliferating OVCAR8 cells that were crosslinked with 1% formaldehyde in PBS for 1 minute on culture plates followed by glycine quenching. Cells were then scraped and counted with 5e5 cells and then incubated with CUT&RUN antibodies. Cross-link reversal was performed using 0.8 μL of 10% SDS and 1 μL of 10 μg/μL Proteinase K and overnight incubation at 55°C. DNA was then purified using the EpiCypher kit. Libraries were prepared by NEBNext Ultra II DNA Library Preparation Kit protocol (NEB #E7645L) and analyzed by Agilent 4200 TapeStation. The libraries were pooled and loaded on a NextSeq 1000/2000 P2 Reagents (100 cycles) v3 flow cell (#20046811) configured to generated paired end reads.