title: GSM6435657: OB/OB 1; Mus musculus; RNA-Seq
library_construction_protocol: Frozen ob/ob and C57BL/6 mouse heads (n=4) were thawed in 30 ml of TRIzol Reagent (Life Technologies) and manually crushed in a mortar Sample Preparation Kit v2 (Illumina Inc., San Diego, USA). In the manufacturer's instructions two modifications were made: In the adapter ligation step 1 μl instead of 2.5 μl adaptor was used; In the library size selection step, the library fragments were isolated using a double Ampure XP purification with a 0.7x beads to library ratio.