title: GSM8000865: SR21,Glucose,72h,rep2; Aurantiochytrium limacinum; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol reagent. oligo dT beads to enrich mRNA with poly A tail. RNA fragmentation and first_x0002_strand cDNAs were generated using random N6-primed reverse transcription, followed by the second_x0002_strand cDNA synthesis. The synthesized cDNA was subjected to end-repair and then was 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments. Many rounds of PCR amplification were performed to enrich the purified cDNA template using PCR primer. Denature the PCR product by  heat and the DNA is cyclized by splint oligo and DNA ligase. DNA nanoball was synthesised.