title: GSM7783394: 37˚C Th0 Replicate 6; Mus musculus; RNA-Seq
library_construction_protocol: CD4+T cells from WT mice were isolated and stimulated for 4 days with αCD3/αCD28 as described above. Cells were washed with cold PBS and RNA extraction was conducted with DNAse treatment according to the RNeasy Plus Mini kit (Qiagen). Poly(A) RNA enrichment was conducted using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), and the sequencing library was constructed by using the NEBNext Ultra II RNA Library Prep Kit (NEB) (P/N: E7765L) following the manufacturer's instructions.  End repair, A-tailing, and adapter ligation was performed to generate the final cDNA library. The library quality was assessed using a Bioanalyzer and quantified using a qPCR-based method with the KAPA Library Quantification Kit (P/N: KK4873) and the QuantStudio 12K instrument.