title: GSM6659886: Delta16HER2_rep2; Mus musculus; ChIP-Seq
library_construction_protocol: 20 mg of tissue were homogenized with a Dounce homogenizer in 0.5 mL of fixation solution (5 mM HEPES pH 7.5, 10 mM NaCl, 0.1 mM Na2EDTA, 0.05 mM EGTA, 1% formaldehyde) and fixed for 10 minutes at 37 °C. Cells were then lysed and subjected to chromatin extraction as previously reported (Amatori S. et al., (2018). Libraries were prepared for sequencing using standard Illumina protocols