title: Transcriptome sequencing of Taurinivorans muris LT0009 grown with sulfolactate [JMF-2012-1-0004A]
design: RNA from triplicate LT0009 cultures grown with 10 mmol/l of taurine, sulfolactate or thiosulfate was extracted using the Analytic Jena innuprep RNA Mini Kit 2.0 following manufacturers instructions. Extracted RNA was subjected to turbo DNAse (Ambion) treatment to remove residual DNA contamination. Ribosomal RNA was not depleted to minimize sample processing biases. Stranded total RNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina following manufacturers instructions.