title: GSM7111326: ap1_h48_mock_atac_r2; Arabidopsis thaliana; ATAC-seq
library_construction_protocol: Plant nuclei were isolated using gentleMACS dissociator in Honda buffer, and then nuclei were sorted by cell cytometry. Nuclei isolation details have been published (Sunaga‐Franze, D.Y., Muino, J.M., Braeuning, C., Xu, X., Zong, M., Smaczniak, C., Yan, W., Fischer, C., Vidal, R., Kliem, M., Kaufmann, K., and Sauer, S. (2021). Single‐nucleus RNA sequencing of plant tissues using a nanowell‐based system. Plant J. 108: 859–869.). 50000 nuclei were used for ATAC-seq. ATAC-seq was done following a published protocol (Buenrostro, J.D., Wu, B., Chang, H.Y., and Greenleaf, W.J. (2015a). ATAC-seq: A method for assaying chromatin accessibility genome-wide. Curr. Protoc. Mol. Biol. 2015: 21.29.1-21.29.9.) ATAC-seq library was done following a published protocol (Buenrostro, J.D., Wu, B., Chang, H.Y., and Greenleaf, W.J. (2015a). ATAC-seq: A method for assaying chromatin accessibility genome-wide. Curr. Protoc. Mol. Biol. 2015: 21.29.1-21.29.9.)