title: GSM8337533: 231_NTC_2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted and purified using only the Zymo Quick-RNA 96 kit. RNA sequencing was performed by the Michigan State University Genomics Core. Sequencing libraries were prepared using the Illumina stranded mRNA library prep kit (Illumina, catalog no. 20040534) with IDT for Illumina RNA Unique Dual Index adapters following the manufacturer's recommendations, except that half-volume reactions were performed. Libraries were assessed for quantity and quality using a combination of Qubit dsDNA HS (Thermo Fisher Scientific, catalog no. Q32851) and Agilent 4200 TapeStation HS DNA1000 assays (Agilent, catalog no. 5067-5584). Libraries were pooled in equimolar amounts, and the pool was quantified using an Invitrogen Collibri quantification quantitative PCR kit (Invitrogen, catalog no. A38524100). The pooled library was loaded onto two lanes of a NovaSeq S1 flow cell, and sequencing was performed in a 1 × 100-bp single-read format using a NovaSeq 6,000 v1.5 100-cycle reagent kit (Illumina, catalog no. 20028316). Base calling was performed with Illumina real-time analysis (version 3.4.4), and the output of real-time analysis was demultiplexed and converted to the fastq format with Illumina Bcl2fastq (version 2.20.0).