title: GSM7699235: 220118_05_NA_CUTTAG_T180_H3K9me2_WT_PFA_st5_rep1; Drosophila melanogaster; OTHER
library_construction_protocol: The snap-frozen embryos were defrosted and resuspended in ice-cold digitonin wash buffer (20mM HEPES (pH7.5),150mM NaCl, 0.5mM Spermidine, 5mM Sodium butyrate, 0.01% Digitonin, 2mM EDTA and 1x Protease inhibitor cocktail). The nuclei were extracted by breaking the embryo using a pestle for 30s. H3 was used as a loading control and for that, after extracting nuclei, the suspension was equally divided into Eppendorf tubes. The rest of the protocol was performed according to Kaya-Okur et al. 2019,  Nat Comm The library was constructed according to Kaya-Okur et al. 2019,  Nat Comm