title: GSM6580953: CDA291V; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted with the Qiagen RNeasy mini and midi kit and was treated with DNase. Total RNA (20 ng) was used to generate libraries with the NEBNext Ultra RNA Library preparation kit (New England Biolabs). Libraries were sequenced in a HiSeq2500 next-generation sequencer (Illumina) to generate 60-base single reads.