title: GSM6720826: Adult NSC rep 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction was performed using the OMEGA Biotek EZNA Total RNA or MicroElute RNA kit according to manufacturer's instructions. RNA was assessed by Fragment Analyzer, and high quality total RNA (RIN > 9.9) was subjected to library preparation using the Illumina TruSeq Stranded mRNA Library Preparation Kit (Cat# 20020594) according to the manufacturer's instructions. Library fragment size was checked using an Agilent Fragment, and then quantified with qPCR using Collibri™ Library Quantification Kit (A38524500, ThermoFisher) on a BioRad CFX96 Touch Real-Time PCR Detection System. Real-time base call (.bcl) files were converted to FASTQ files using Illumina bcl2fastq2 conversion software