title: GSM6500567: ∆nhr-114-veh-3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted with TRIzol (Life Technologies). The RNA quality and quantity were determined with a NanoPhotometer® spectrophotometer (IMPLEN) and a Qubit® RNA Assay Kit in a Qubit® 2.0 Fluorometer (Life Technologies). The integrity of the RNA was assessed using the RNA Nano 6000 Assay Kit of a Bioanalyzer 2100 system (Agilent). Sequencing libraries were generated using an NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's recommendations, and index codes were added to attribute sequences to each sample.Clustering of the index-coded samples was performed on a cBot Cluster Generation System using a TruSeq PE Cluster Kit v3-cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina NovaSeq 6000 platform, and 125 bp/150 bp paired-end reads were generated.