title: GSM7784171: 7363M; Homo sapiens; RNA-Seq
library_construction_protocol: Tumor tissue was thoroughly diced with a sterile scalpel and placed in a GentleMACs C-tube containing one milliliter of tissue digestion media. Tissue was mechanically digested on the GentleMACs dissociator five times under the human tumor tissue program “h_tumor_01”.The remaining solid tissue in the strainer, enzymatic sample, was collected and placed back in the C-tube with 4 mls of tissue digestion media, 0.5 ml of 3000 U/ml collagenase/ 1000 U/ml hyaluronidase. After FACS sorting for live cells, library prep was performed using Chromium Next GEM Single Cell 3' Kit v3 (10x Genomics, San Francisco, CA) according to manufacturer's instructions and send to Medgenome for sequencing on the Novaseq.