title: GSM8073382: RNAseq, Mix, Exp2, PW, Control, Rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 20 third-instar larval wing discs and 15-20 pupal wings (24-27h APF) per replicate were rapidly dissected in Schneider medium on ice. Total RNA was extracted with TRIzol reagent and purified using the RNA Clean & Concentrator™-5 kit (Zymo Research, cat. R1015). >1μg of total RNA was sent to Novogene for strand-specific poly-A enrichment mRNA-seq library preparation. Paired-end sequencing of the libraries was performed on Novogene's NovaSeq 6000 PE150 platform.