title: GSM8034867: NCCIT; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was isolated by the TRIzol reagent according to the manual (Qiagen, Hilden, Germany). RNA concentrations and purities were measured by the Nanodrop 2000 photo-spectrometer (260 / 280 nm; 260 / 230 nm) and RIN numbers were detemrined by Fragment Analyzer measurememt. 200 ng of total RNA were used for library preparation and microRNA sequencing (microRNAseq). Library preparation (NEXTFLEX Small RNA-Seq Kit v4' (Revvity, Hamburg, Germany)) and microRNAseq (NovaSeq6000 SP 2 x 50 bp) has been carried out at the 'Competence Centre for Genomic Analysis' (Kiel, Germany).