title: GSM6579125: EpiLC_rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from the samples according to the manufacturer's instructions using the RNeasy kit (74004, Qiagen). 1 µg RNA was used as input for the library preparation. Prior to the construction of the library, the integrity and quality of the RNA were evaluated using a 4200 Agilent TapeStation device and RNA ScreenTape reagents (Agilent). Samples having a RIN > 7.0 for RNA integrity were used. The library was generated with the TruSeq Stranded mRNA capture kit (20020594, KAP Biosystems) following the manufacturer's instructions, with the exceptions listed below. Unique Dual-Indexed (UDI, KAPA biosystems) adapters were ligated and the libraries were amplified for 8 cycles. Quality and concentration were measured using a 4200 Agilent TapeStation instrument and D5000 ScreenTape reagents (Agilent). Accel-NGS Methyl-Seq DNA Library Kit (manufacturer's recommendation)