title: targeted methylome-seq of homo sapiens: brain
design: Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit. Samples were denatured to single-stranded DNA with single strand-based SRSLY Nano Library Prep kit followed by adapter ligation and indexing PCR steps. In the indexing PCR step, uracil tolerant HiFi PCR Master Mix was used. Target enrichment was performed using Twist Human Methylome Panel.