title: SCA-140
design: Biliary stents were scraped to separate the biofilm matrix from the stent structure. The biofilm matrix was homogenized in 550 l of Cell Suspension Solution and DNA was subsequently extracted using a GNOME DNA isolation kit MP Biomedicals 112010600 following a published protocol (Furet et al. 2009). Sequencing libraries were prepared using the Nextera DNA Flex Library Preparation Kit (Illumina), following the manufacturers guidelines. Sequencing was performed on the Illumina NovaSeq 6000 platform following manufacturers protocols