title: GSM6435618: Control_2, Day 21; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction was performed using a Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, US), according to the manufacturer's protocol. The RNA-Seq was carried out at the UCL Genomics facility. RNA concentration was measured on a Nanodrop spectrometer and 500 ng of RNA was used for cDNA synthesis and library preparation using the Kapa mRNA HyperPrep assay (Roche, Basel, Switzerland) and xGen UDI-UMI adapters (Integrated DNA Technology, Shanghai, China) , according to the manufacturer's protocol. Sequencing of these libraries was performed on the NextSeq 2000 (Illumina, San Diego, US) P2 flow-cell with 56bp paired-end reads.