title: GSM8000820: Syngap1+/-: CSP-TTK21 (R2); Mus musculus; RNA-Seq
library_construction_protocol: Trizol method. Briefly, Mice were sacrificed by cervical dislocation; the dorsal hippocampus was dissected out and chopped finely with a razor blade. All reagents and instruments used for RNA isolation were DEPC treated. Samples were homogenized in TRIzol reagent (Invitrogen). RNA was isolated by the traditional phenol: chloroform method. After phenol-chloroform treatment, the aqueous phase was collected, and ethanol precipitated using absolute ethanol. The pellets were washed twice with 70% ethanol and air-dried. RNA pellets were resuspended in RNase-free water.  DNase treatment was done for 30 min at 37 ºC. RNA samples were further purified by chloroform/phenol extraction and ethanol precipitated. Pellets were washed twice with 70% ethanol and resuspended in RNase-free water at 65 ºC for 10 mins. RNA concentration was determined by Nano-drop As per Illumina recommended Protocol