title: GSM6426726: Luc knockdown small RNA-seq-4; Aedes aegypti; miRNA-Seq
library_construction_protocol: Three days post-transfection, Aag2 cells were lysed in 1 mL RNA-Solv reagent (Omega Bio-tek), followed by RNA extraction through phase separation and isopropanol precipitation. RNA integrity was evaluated on EtBr stained agarose gels, and RNA concentration was determined using the Nanodrop ND-1000. Small RNA deep sequencing libraries were generated using the NEBNext Small RNA Library Prep Set for Illumina (E7560, New England Biolabs), using 1 µg RNA as input. Library preparation was performed in accordance with the manufacturer's instructions except for 3' adapter ligation, which was performed for 18 h at 16°C to enhance the ligation efficiency of 2'-O-methylated small RNAs. Libraries were sequenced on an Illumina Hiseq4000 machine by the GenomEast Platform (Strasbourg, France).