title: GSM8208602: OC + Paeoniflorin #2; Mus musculus; RNA-Seq
library_construction_protocol: Following compound administration for 4 weeks, to create a post-ovulatory state, mice were induced by intraperitoneal injection of 5 IU of pregnant mare serum gonadotropin (PMSG; HOR-272; Prospec, Rehovot, Israel), followed by an injection of 5 IU of human chorionic gonadotropin (hCG; HOR-250; Prospec) 48 hours later. Ovaries were removed from mice and weighed at 14h-16h post-hCG administration. The ovary was immediately preserved in liquid nitrogen for RNA sequencing.  Each left ovary (n=3) of a post-ovulation mouse was immersed in 300ul TRIzol (Invitrogen, Carlsbad, CA, USA) and homogenized using a disposable grinder (Biomasher II; 890863; LMS Co., Tokyo, Japan). Total RNA was isolated using Trizol reagent (Invitrogen). The quality of purity and integrity of the extracted RNA samples were analyzed using a NanoDrop ND-2000 Spectrophotometer (Thermo Inc., DE, USA) and Agilent 2100 bioanalyzer (Agilent Technologies, Amstelveen, The Netherlands). RNA library was constructed using QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions.