title: GSM7111649: retinal ganglion cell, Control subject 3 replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy mini kit (Qiagen74104) from ethanol fixed and then FACS-sorted BRN3+ immunolabeled cells at day 40-43 of differentiation. For each prep at least 60 ROs were pooled. Random primed sequencing libraries depleted of ribosomal RNA were constructed from 50 ng RNA using KAPA Stranded RNA-Seq Kit with RiboErase (HMR) (KAPA Biosystems) according to instructions except those volumes were halved and an extra 1.2X KAPA bead library cleanup was performed to remove adapter dimers prior to 13 cycles of amplification.