title: GSM6585292: L36_sip63_H3K27ac_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: PDX tissue samples were homogenized on dry-ice using the Sciences BioMascher® II Micro Tissue homogenizers. Powdered tissue and cell pellets from L3.6pl cells were fixed in 1% formaldehyde in PBS for 20 min and quenched with 125 mM glycine for 5 min at room temperature. Sonicated chromatin extract was immunoprecipitated overnight with corresponding antibodies. Protein A sepharose was used to capture immune complexes. Immune complexes were washed, de-crosslinked and DNA was extracted. ChIP-seq libraries except the H3K4me1 and H3K4me3 were prepared using the KAPA HyperPrep (Roche) kit. H3K4me1 and H3K4me3 libraries were prepared using the MicroPlex Library Preparation Kit (Diagenode) according to the manufacturer's instructions. The library size was estimated using a Bioanalyzer 2100.