title: RNA-Seq of Ornithoptera alexandrae: adult male FC560 specimen
design: RNA Extraction and purification were conducted with the Qiagen RNeasy kit. We used part of the thorax and part of the abdomen (adult specimen) that were crushed in the lysis buffer. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and BioAnalyzer for size distribution detection. Quantified libraries have been pooled and sequenced on Illumina platforms, according to effective library concentration to a data amount of about 8 Gb per sample