title: GSM7794460: c587ts-esg-Dam; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: Testes from esg-Dam, Stat92E-Dam and UAS-Dam (control) were dissected in cold 1XPBS and immediately preserved on dry ice and kept at -80°C. Genomic DNA was then isolated. Genomic DNA was treated by DpnII and alkaline phosphatase.The treated samples were digested with DpnI and ligated with adaptor for ligation-mediated polymerase chain reaction (LM-PCR). Following PCR, the amplified DNAs were treated with T7 exonuclease and purified for sequencing following fragmentation.