title: GSM7078341: WT OT-I, tumor, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Library generation was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics). Briefly, T cells were loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA cell lysate in each GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and a 10x Barcode. Barcoded and pooled cDNA was cleaned up with DynaBeads, amplified by PCR and the appropriate size fragments were selected using SPRIselect beads for downstream library construction. During  library construction, Ilumina primer sequences and a sample index were added for paired-end sequencing.