title: Illumina WGS of Xanthomonas oryzae pv. oryzae: Genome Sequencing
design: 1. Construct the library with a starting amount of 1 g DNA.2.Covaris M220 ultrasonic breaks DNA to 300~500bp3. Fill up, add A to the 3 end, and connect the index adapter (TruSeq Nano DNA Sample Prep Kit)4. Library enrichment, 8 cycles of PCR amplification5.2% agarose gel to recover the target band (Certified Low Range Ultra Agarose)6. TBS380 (Picogreen) is quantified and mixed according to the data proportion and put on the machine7. Perform bridge PCR amplification on cBot solid phase carrier to generate clusters8. Illumina novaseq6000 sequencing platform, performs 2*150bp sequencing