title: GSM8070828: T2; Homo sapiens; RNA-Seq
library_construction_protocol: After pre-treatment, cells were treated  for 1, 4 and 24 hours with either,- no treatment (NT), Recombinant Human IL-6 Protein (IL-6) 1 ng/ml (low dose), IL-6 20 ng/ml (high dose, cat# 206-IL-010, R&D Systems, Minneapolis, MN), Hyper IL-6 (Recombinant human IL-6R alpha/IL-6 chimera) 1 ng/ml (low dose), and Hyper IL-6 20 ng/ml (high dose, cat# 8954-SR-025, R&D Systems, Minneapolis, MN). Untreated HepG2 control cells were washed twice with PBS and provided with fresh medium. The following day, cells were used for protein and RNA isolation. Sequencing libraries were generated using with 1 ug of total RNA by NEBNext Ultra RNA Library Prep Kit for Illumina (Cat. no-E7530LNew England Biolabs).