title: GSM8372148: BVDV Persistent Infection: 4 Months Postnatal PBMC DNA 144; Bos taurus; Bisulfite-Seq
library_construction_protocol: DNA was extracted from WBCs processed from whole blood using Qiagen DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). DNA samples were digested with 30 units of MspI, fragments purified with DNA Clean & Concentrator-5  and ligated to adapters with replacement of cytosine with 50-methyl-cytosine according to Illumina's guidelines. Fragments greater than 50 base pairs were recovered and bisulfite treated using the EZ DNA Methylation-Lightning Kit . Samples were subjected to PCR with Illumina indices. Size and concentrations of purified products were confirmed with the Agilent 2200 TapeStation prior to sequencing on an Illumina platform