title: GSM8122799: Giredestrant-treated, GPX4 guide 2, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using Qiagen RNeasy Plus Mini kit (Cat. No. 74134), For sequencing library generation, the Truseq Stranded mRNA kit (Illumina) was used with an input of 100-1000 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies). Libraries were pooled and sequenced on NovaSeq 6000 (Illumina) to generate 30 million single-end 50-base pair reads for each sample.