title: GSM6720798: Caco-2, mock, 4h, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from infected or mock-treated Caco-2 cells at 0, 1, 2, 4, 7, 12, 24 and 48  hpi using the Qiagen RNAeasy plus extraction kit (Qiagen, Hilden, Germany). For quantifying SARS-CoV-2 genome abundance in mock samples, cDNA was made using  iSCRIPT reverse transcriptase (BioRad, Hercules, CA, USA). q-RT-PCR was performed  using iTaq SYBR green (BioRad) as per manufacturer's instructions, TBP was used as  housekeeping gene and for normalization (COV1 primers: for 5'-  GCCTCTTCTGTTCCTCATCAC-3', rev 5'-AGACAGCATCACCGCCATTG-3'; TBP  primers: for 5'-CCACTCACAGACTCTCACAAC-3', rev 5'-  CCACTCACAGACTCTCACAAC-3'; Eurofins, Luxemburg). RNA samples were stored at -  80°C. DNA libraries were prepared at GeneWiz Inc. (Leipzig, Germany) using the NEBnext  Ultra II RNA directional Kit (New England Biolabs GmbH, Frankfurt, Germany). Paired-end  sequencing of 2×150bp was performed at GeneWiz Inc. using an Illumina NovaSeq 6000  instrument (Illumina, San Diego CA, USA).