title: GSM6580977: Blood_HDL_naive_IGM; Homo sapiens; OTHER
library_construction_protocol: RNA was isolated using RNeasy Plus Kits (Qiagen) and transcribed into cDNA using random hexamer primers and Superscript III® (both Invitrogen). Amplicon-seq: IGH transcripts of the SLO samples were amplified from 5µl cDNA per reaction in a multiplex PCR using the forward VH1-6 FR1 (BIOMED-2) primers and the CgCH1, the IGHA or the IGHM reverse primer (sequence available upon request). These PCR products were purified and sequenced using Roche 454 sequencing as previously described Amplicon-seq: IGH transcripts of the PBMC samples were amplified using the forward VH1-6 FR1 (BIOMED-2) primers and costum IGHG, IGHA and IGHM reverse primers (sequence available upon request). PCR products were purified and sequenced using 2x300 Illumina sequencing (Illumina).