title: GSM6500409: Control2B; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from FACS-sorted cells was isolated using the Arcturus PicoPure RNA Isolation Kit (Applied Biosystems, #KIT0204) according to the manufacturer's protocol. Sorted O4+ cells were resuspended in 100 ml RNA extraction buffer and incubated at 42°C for 30 min. Samples were spun down and the RNA-containing supernatant further purified of residual genomic DNA by on-column digestion with RNase-free DNase (Qiagen, #79254). RNA integrity was measured with the Agilent 2100 BioAnalyzer, and only RNA with RNA integrity number (RIN) above 7 was used for sequencing. Library preparation and sequencing was conducted by experienced personnel at the John P. Hussman Institute for Human Genomics (Miller School of Medicine, University of Miami) using an Illumina NovaSeq sequencing system. Paired-end, 125 base pair reads were generated.