title: GSM7159429: TH17 cells, DMSO, Day5 [TH17-DMSO-3]; Mus musculus; RNA-Seq
library_construction_protocol: T cells were harvested on Day5 and total RNA were extracted using TRIzol™ Reagent. RNA purity were measured by Nano Drop. The concentration of RNA was determined by Qubit RNA Br (broad-range) Assay Kit, and the integrity of RNA was detected by RNA ScreenTape. mRNA were isolated using the NEBNext® Poly(A)mRNA Magnetic Isolation Module kit. The mRNA library was constructed with the NEBNext® Ultra™ II mRNA Library Prep Kit for Illumina® sequencing platform.