title: P. pseudocerasus  RNAseq leaf
design: Total RNA was extracted from three tissues (leaf, stem and root) with the RNA extraction Kit (QIAGEN, Germany). High-quality cDNA libraries were prepared with TruSeq Stranded mRNA Sample Preparation Kit and sequenced on the Novaseq 6000 platform by the Novogene (Beijing, China). Quality control was performed by the fastp software1. As a result, an average of 6.94 Gb of high-quality RNA-seq data per tissue was used for transcript evidence analysis in the context of gene structure annotation for the Duiyingtao genome