title: GSM6500678: Quiescent MuSCs, Pax7-YFP, rep3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using TRIzoL (Qiagen). 187 to 800ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext Ultra II RNA library prep kit, and NEBNext Poly(A) mRNA Magnetic isolation module (Cat# E7770 and E7490; New England Biolabs) following manufacturer's protocols.