title: GSM8035464: COL1A1_mini_L1, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Control, PDGFB- or TGFb1-induced microHOs (n>30 per group) from 4 batches were harvested on day 21, and single cell suspensions were prepared by protease digestion The single cell suspensions were visually inspected under a microscope, cells were counted using a Scepter™ 2.0 Handheld Automated Cell Counter (EMD Millipore, Burlington, MA), and then resuspended in PBS with 0.01% BSA. scRNA-seq libraries were prepared by using the spit-pool based scRNA-seq method (Evercode™ WT Mini v2 from Parse biosciences) according to the manufacturer's instructions. In brief, 1668 cells from each sample were loaded in one wells for the first round of sample barcoding, and the final pooled cells were divided into two equal libraries for sequencing.