title: GSM6659335: ebp1_SEC#2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA from the protoplasts was extracted using the RNeasy™ Micro Kit (QIAGEN) according to the manufacturer's instructions. RNA was eluted in 14-μl water, and RNA quality was monitored using a Bioanalyzer Pico chip (Agilent Technologies). cDNA was generated using SMART-Seq V4 Ultra Low Input RNA Kit (Clontech) with 18 amplification cycles, and RNA-seq libraries were generated using the TruSeq RNA Sample Prep Kit v2 (Illumina) according to the manufacturer's manual. The libraries were qualitatively and quantitatively verified and then loaded onto a flow cell for cluster generation using cBot and TruSeq PE Cluster Kit v3 (Illumina).