title: RNA-Seq of A549 cells
design: Total RNA was extracted using TRIzol reagent (Thermo Fisher Scientific, USA) according to the manufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions. The libraries were sequenced on an Illumina HiSeq X Ten platform and 150 bp paired-end reads were generated and analyzed (OE Biotech Co., Ltd, Shanghai, China).