title: RNA-Seq of human adipocites
design: Total RNA was isolated with Total RNA Purification Plus Kit (Norgen Biotek, Canada). RNA quality and quantity was assessed by Agilent 2100 Bioanalyzer and samples stored at -80C until use. Total RNA (2 g) was used to prepare the library for Illumina sequencing. Single-end reads (60M per sample) were produced by Illumina HiSeq 2013