title: GSM6720547: cdHFD_female_ZT2_rep_3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from livers using Trizol reagent (Thermofisher, Germany) and columns (Zymo Research, USA) according to the manufactory's instructions. Trueseq RNAseq comprising 40 million reads per sample (12GB) was performed at Novogene (Germany). Samples underwent quality control and only those with RNA integrity number (RIN) higher than 6.5 were used. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. The clustering of the index-coded samples was performed according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.