title: GSM8073024: WT_C1_LPS_rep2; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq: Total RNA was extracted from sorted cells using TRIzol (Invitrogen, cat#15596018) according to the manufacturer's protocol. For ATAC-seq: For ATAC-seq analysis, 5×104 splenic cDC1s and cDC2s were resuspended in 35 μl of lysis buffer (10 mM Tris-Hcl PH7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630) and incubated on ice for 10 minutes with vortex 3 times. After adding 10 μl of TTBL and 5 μl of TTE Mix, the mixture was shaken at 37°C for 30 minutes for DNA fragmentation. RNA-seq library was constructed for deep sequencing by BGI Genomics (in vivo) or Novogene (in vitro).  For ATAC-seq: Fragmented DNA was purified by 1.8-fold of AMPure beads, barcoded with dual indexes and amplified by PCR. Size selection and purification of DNA were performed using 0.4-fold and 1.6-fold of AMPure beads, respectively. The size distribution and molarity of library DNA were determined by Agilent 2100 bioanalyzer and Qubit.