title: 16S SSU rRNA V4 region of soil sample : 189B
design: To identify the bacterial community, we amplified the V4 region of the 16S SSU rRNA with the forward 515F (5'-GTGCCAGCMGCCGCGGTAA) and reverse 806R (5'-GGACTACHVHHHTWTCTAAT) primers (Caporaso et al., 2012). We performed the amplification on 1 L of template DNA in a volume of 25 L using Taq PCR Master Mix Kit (QIAGEN). The program was as follows: 3 min at 94C, 35 cycles of 1 min at 95C, 1 min 50C and 1 min at 72C, and a final step 10 min at 72C.