title: GSM8122115: WT-male-PTX-DRG2; Mus musculus; RNA-Seq
library_construction_protocol: RAA was harvstd using Qiagen mini prep kit. 6~7 ug of total RNA was used for the construction of sequencing libraies. Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification. After purification, poly(A) RNA was fragmented using divalent cation buffer in elevated temperature.