title: GSM6865694: WT-PEPD-mix_CITE-TSA 794105_AF21; Mus musculus; OTHER
library_construction_protocol: All mice are on a C57BL/6 background. For the scRNAseq analysis of chimeric T cells, magnetically isolated T cell populations from 4 WT:WT and 4 WT: Pepd-/- BM chimeric mice were hashtagged by samples for multiplexing using Biolegend TotalSeq-B hashing antibodies by protocol (B0301 #155831, B0302 #155833, B0303 #155835, B0304 #155837, B0305 #155839, B0306 #155841, B0307 #155843, B0308 #155845). Pooled hashtagged CD45.2+ T cells were isolated by FACS, then labelled with  TotalSeq-A antibodies by protocol (anti-CD4 #100569, anti-CD8a #100773, anti-CD279 (PD-1) #109123, anti-CD44 #103063, anti-CD62L #104451, anti-CD25 #102055, anti-CD127 (IL7R), #135045, anti-ICOS #313555, anti-CD69 #104546, anti-CD27 #124235, anti-CD5 #100637, anti-KLRG1 #138431, anti-CD95 #152614,, then sequenced as above.  After washing, cells were loaded into the chromium controller (10X-Genomics) chip and sequenced as described. Cells were loaded into the chromium controller (10×-Genomics) chip following the standard protocol for the chromium single cell 3' kit. (Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon., Kendel et al. Scientific Reports., 2020) Total-Seq-A ADT and Total-Seq-B Hashtag-seq as previously described following biolegend protocols (Multi-omic single cell analysis resolves novel stromal cell populations in healthy and diseased human tendon., Kendel et al. Scientific Reports., 2020) 3' GEX, Total-Seq-A ADT, Total-Seq-B HTO