title: GSM8122126: Rice root cells - bulk-Meristem-1; Oryza sativa; RNA-Seq
library_construction_protocol: Sections (root tip to end of lateral root cap: Meristem; end of lateral root cap to the start of root hair elongation: Elongation; 1mm each beyond the start of root hair elongation: Maturation1 and Maturation2) were harvested into 10 μL RNA-later (Ambion) in the lid of a 1.5 ml tube. Samples were frozen in liquid nitrogen and stored at −80.and then processed by grinding with a blue homogenization pestle. RNA was isolated using the Zymo MagBead RNA Isolation kit according to the manufacturer's protocol (Zymo). RNA was used as input into the Lexogen QuantSeq 3′ FWD RNA-Seq library preparation procedure according to the manufacturer's protocol, using the Unique Molecular Identifier (UMI) PCR add-on kit. Libraries were indexed and pooled on an Illumina NextSeq. Lexogen QuantSeq 3′ FWD RNA-Seq