title: GSM7076001: RWPE1-ERG, PABPC1 ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 15 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor Pico waterbath sonicator (Diagenode). Illumina TruSeq barcode adapters were ligated to repaired ends of sheared fragments. Adapter-ligated fragments were PCR amplified and purified with Ampure XP beads and size selection from a 2% agarose gel to remove adapter dimer contamination. Libraries were pooled and validated using the Agilent D1000 TapeStation.