title: GSM8370831: Chondrocytes, treated, H12, hypoxia, R1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated by using TRIzol reagent (Life Technologies) and further purified (RNeasy Mini kit, Qiagen). At each step, RNA was checked on a bioanalyzer (RNA 6000 Pico Kit, Agilent) and quantified on Qubit. Purification was performed with the Ribo-Zero Magnetic Gold kit (Tebu Bio) to remove ribosomal RNA, followed by another purification with the RNeasy MinElute Cleanup Kit (Qiagen). cDNA libraries were constructed by using the TruSeq RNA Sample Prep kit (Illumina). Three barcoded cDNA libraries were made for each condition, at each time point.