title: V3V4 region of the 16S rRNA gene of microbiota human stool sample
design: Sequences were derived from PCR amplification of the V3V4 region (341F (5'CCTACGGGNGGCWGCAG-3') and 806R (5'-GACTACHVGGGTATCTAATCC-3') (Klindworth et al., 2013)) of the 16S rRNA gene from whole stool samples of healthy individuals. Library preparation was performed according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol (Illumina, CA, USA) with Q5 High-Fidelity DNA Polymerase (New England Biolabs). Sequencing was performed on the Illumina NextSeq platform (2x300 cycles, 10% PhiX).