title: GSM8208909: Germ cells from 15-day C57BL/6 cKO mutant 4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from germ cells using RNAzol (Sigma Aldrich), quantitated by Nanodrop ND 1000 spectrophotometer and the quality determind by RQN values using Bioanalyzer software on a Pico RNA Chip performed by the Genomics Core at Albert Einstein School of Medicine.. RQN values were all 10. The RNA was sent on dry ice to Novogene. All samples passed NovaSeq PE150 quality control, poly A+ RNA was prepared by Novogene, 8 non-stranded mRNA libraries were prepared and subjected to paired-end RNA-seq. Non-stranded mRNA-Seq libraries (poly A enrichment) were prepared by Novogene for 150 base pair paired-end (PE) sequencing on an Illumina HiSeq platform.