title: Pacbio_Seq of Pseudoliparis sp.2 : muscle
design: Genomic DNA was extracted from muscle tissue  using the Qiagen DNeasy Blood & Tissue Kit. DNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer and Qubit fluorometer. SMRTbell libraries were constructed using the PacBio SMRTbell Express Template Prep Kit 2.0 and size-selected using BluePippin to enrich for fragments >10 kb. Sequencing was performed on the PacBio RS II platform to generate long-read sequences.