title: Genomic sequencing of E. coli natural isolates to identify methylated bases
design: Genomic sequencing of E. coli natural isolates to identify methylated bases
library_construction_protocol: Cell harvested at mid-exponential phase unless otherwise specified (e.g. at 96 hours) Minimal glucose media (M9) or rich media (LB) grown at cold stress (25C), no stress (37C), or heat stress (42C) Promega Wizard kit Oxford Nanopore RBK-004