title: GSM7626465: testicular tissue, HO-1-1-1 (lncRNA+circRNA); Rattus norvegicus; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from cells/tissues using the Trizol (invitrogen) according to the manufacturer's protocol. RNA purity was assessed by ND-1000 Nanodrop required A260/280≥1.8, A260/A230≥2.0. RNA integrity (RIN) was evaluated by Agilent 2200 TapeStation (Agilent Technologies, USA) requiring RIN≥7.0. Briefly, rRNAs were removed from Total RNA using Epicentre Ribo-Zero rRNA Removal Kit (illumina, USA) , then RNA was treated with RNase R (Epicentre, USA) and fragmented to approximately 200bp. Subsequently, the purified RNA fragments were subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycle according to instructions of NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB, USA). The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit®2.0(Life Technologies, USA) and then sequenced on HiSeq 3000 with 2 X 150 bp mode.