title: GSM8163413: plasmid DNA fragments, amplified, after acetic screening; synthetic construct; OTHER
library_construction_protocol: Plasmid extraction was performed using ZymoprepTM Yeast Plasmid Miniprep II (Zymo Research Corporation). Sequences containing spacers in sgRNA plasmids were amplified via PCR, purified, fragmented, and their ends were repaired and reformed to become clusters.