title: GSM8427807: H720-NU7441-1, 5days; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were grown in RPMI 1640 media supplemented with 10% FBS, 1% pen-strep. And 1X Glutamax, in 37 degree C, 90% Humidity and 5% carbon-di-oxide. 2 X 10^6 cells were plated 24 hours before treatment and then were treated with either DMSO or 4µM NU7441. Sequencing libraries were clustered on one flowcell lane and then loaded on the Illumina HiSeq instrument (4000 or equivalent) according to manufacturer's instructions. Samples were sequenced using a 2x150bp paired end (PE) configuration. Image analysis and base calling were conducted by the HiSeq Control Software (HCS). Raw sequence data (.bcl files) was converted into fastq files and de-multiplexed using Illumina's bcl2fastq 2.17 software. One mismatch was allowed for index sequence identification.