title: GSM7965639: BALB-c-Gexp, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Monocytes were isolated from the freshly collected spleens of sham-operated and CLP-surviving mice using EasySep™ Mouse Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer's protocol. Isolated monocytes were blocked with rat anti-mouse CD16/32 antibody (BioLegend), followed by staining with PE-Cy7-anti-CD11b and FITC-anti- Ly6C antibodies and FVD efluor506 with Total Seq™-B 0302 anti-mouse Hashtag 2 Antibody or TotalSeq™-B0303 anti-mouse Hashtag 3 Antibody or TotalSeq™-B0304 anti-mouse Hashtag 4 Antibody or TotalSeq™-B03050 anti-mouse Hashtag 5 Antibody, or TotalSeq™-B0306 anti-mouse Hashtag 6 Antibody (BioLegend). Five different Total Seq™-B antibodies were used for BALB/c and C57BL/6, respectively, to distinguish them in the later analysis. After combining cells from 2 sham-operated or 3 CLP-surviving mice, CD11b+ Ly6C high populations were sorted using BD FACSAria IIu (BD Biosciences). Cells were put onto the 10X Chromium Controller (10X Genomics). Gel Bead-in Emulsions were generated using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10X Genomics), following the manufacturer's recommendations.