title: GSM6580963: TIE:EGFP MCR:MCS Tumor 1 GFP+ #3; Danio rerio; RNA-Seq
library_construction_protocol: Tumors were excised and dissociated for 30 minutes with occasional chopping using 0.075mg/mL liberase (Sigma #5401119001) in DMEM (Gibco #11965-092) with 1% Penstrep (Corning #30-002-CI). Dissociated samples were filtered through a 40µm filter and resuspended in FACs buffer (PBS/ 10%FBS/1% Penstrep) before filtering through a FACs tube (Corning #352235). Single cells were sorted into 384-well-cell-capture plates containing barcoded primers from Single Cell Discoveries (https://www.scdiscoveries.com/) using a BD FACS ARIA II sorter. Sorted plates of GFP+ cells and GFP-;mCherry+ cells. Library preparation and Illumina sequencing was performed by Single Cell Discoveries. SORT-seq