title: GSM8337109: Amel_adult_antenna_dsx_stop_stop_worker_biol rep3; Apis mellifera; RNA-Seq
library_construction_protocol: RNA was isolated from the antennae of workers with the dsx stop/stop mutation and wild-type workers using TRIZOL reagent as described in Vleurinck et al., 2016. RNA extracts from 5 bees were pooled for each of 3 biological replicates and genotype. Library were prepared using 500 ng RNA and the "VAHTS Universal RNA-Seq Library Prep Kit for Illumina V6 with mRNA capture module version 7.0" (Vazyme Biotech co.). After normalisation of the bead-purified libraries, sequencing was performed on the HiSeq 3000/4000 system (Illumina Inc.) with a read setting of SR 1x150 bp.