title: GSM8410641: E11.5_Rep2_PGC_Fem_H3K27me3-Part2; Mus musculus; OTHER
library_construction_protocol: genomic DNA DNA was isolated with antibody and PA-Mnase incubation. Library preparation was performed according to manufacturer's instructions (NEBNext ® Ultra™ II DNA Library Prep Kit for Illumina) using the following modified library amplification program: 98 °C for 30 s (98 °C for 10 s, 65 °C for 15 s) × 15 cycles, 65 °C for 5 min), hold at 4 °C. Average library size and quality control were performed using a Fragment Analyzer (High Sensitivity NGS kit) and qPCR (Roche Light Cycler 480).