title: GSM7635054: KO, GEX, L2; Mus musculus; OTHER
library_construction_protocol: Sorted splenic NK cells from Reg-1fl/fl and Reg-1ΔNK were washed twice with PBS and suspended in PBS supplemented with 400 μg/mL non-acetylated bovine serum albumin (Sigma Aldrich, B6917). Cells were labeled with selected oligo barcode conjugated antibodies. Individual cells were then coupled to beads using the 10X Genomics Chromium controller (10X Genomics). Single-cell sequencing libraries were prepared following the 10X Genomics Protocol using 5' Reagent Kits v2 chemistry and sequenced to a median depth of approximately 20,000 reads per cell using an Illumina NovaSeq 6000 on rapid run.