title: Transcriptional differential expression between WT and nkx2.7 morphants at shield stage
design: Transcriptional differential expression between WT and nkx2.7 morphants at shield stage
library_construction_protocol: 10 ug of total RNA was isolated at shield stage embryos and samples were prepared according to the Genomic Services Group (Wellcome Trust Centre for Human Genetics, University of Oxford) requirements for library preparation. Nkx2.7 ATG morpholino (injected at 4ng) and WT siblings Total RNA was isolated from 200 embryos per sample at shield stage using the RNAeasy Micro kit (Qiagen). RNA sequencing libraries were constructed from 200ug total RNA isolated using TruSeq RNA Sample Prep Kit (Illumina)