title: GSM7111741: REH DUX4-IGH rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction using PureLink RNA Mini Kit (catalog # 12183025, Life Technologies), following manifacturer's protocol. cDNA synthesis using SuperScript® III First-Strand Syntesis SuperMix for qRT-PCR (cat. #11752-250, Life Technologies) NGS libraries were generated with the mRNA Stranded TruSeq protocol. Libraries were barcoded, pooled and sequenced on IlluminaNextSeq 500 sequencing system in single-end reads mode, with reads 76nt long.