title: GSM8212432: Control, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Tumor were harvested from 5 individual mice in each treatment group and pulled together to digest and process for signle cell suspension with a cocktail of Collagenase-III, Dispase-II, and Deoxyribonuclease. Following enzymatic digestion, the cells were FACS sorted as live non-tumor (GFP-) population. For multiplexing, cells were split into separate tubes as either mCherry+ or mCherry- cells (mCherry is a fluorescence reporter included in both the empty vector and Zbtb46 overexpressing vector), marked with distinctive hashtags (Hashtag-B0301 for mCherry+ and Hashtag-B0302 for mCherry- cells), and pulled back to make two samples: control and treatment. The cells were then processed for single-cell library generation using version 3.1 Chromium Single Cell 3' v3.1 HT library, Gel Bead & Mutiplex Kit (10X Genomics), and sequenced with Illumina NovaSeq 6000 The library construction protocol was the 10X Genomics single cell 3` version 3.1 HT kit performed according to manufacturer's protocol