title: GSM7794586: CD4T, KO ATAC rep2; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq was performed using the protocol from Corces et al., 2015 with small modifications. Nuclear pellets at 5x10^4 were permeabilized and tagmented by Tn5 enzyme. Tagmentation was performed using Nextera DNA library preparation kit (illumina #15028212) according to manufacturer's instructions. Reverse-crosslinked DNA was amplified using Nextera PCR primers. Libraries were paired-end sequenced on Illumina HiSeq X ten platform.