title: GSM7643724: Transduced with DNAJB1::PRKACA sample 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from PHH cells was extracted using the RNeasy Mini Kit (Qiagen). RNA concentration levels were measured in a Nanodrop 2000c (Thermofisher) using the 260/280 ratio. RNA Integrity Values (RIN) were measured in an Agilent BioAnalyzer and TapeStation. Paired-end 2x150nt RNA-seq libraries were prepared using TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero (Gold) for rRNA and mt-rRNA depletion (Illumina).