title: GSM8072567: Vehicle under 3.9 mM glucose, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: The islets were collected in microcentrifuge tubes, centrifuged at 100g for 1 minute, and washed with 1mL PBS. The islets were incubated in 700 μL Accutase for 5 minutes at 37℃, then gently pippeted 30 times using 1mL pippete. The reaction to disperse the islets was stopped by adding RPMI1640 containing 3.9 mM or 11.1 mM glucose, 30% FBS. The single-cell suspensions were centrifuged at 300g for 5 minutes, the supernatants were gently removed, then 1mL RPMI1640 containing 10% FBS and 3.9 mmol/LM or 11.1 mM glucose was added. Approximately 10000 cells per sample were loaded onto a 10X Chromium Controller for GEM formation and cell barcoding using Next GEM Single Cell 30 v3.1 reagents. Barcoded single cells were subjected to cDNA synthesis and sequencing library construction using 10X Next GEM Single Cell 30 v3.1 reagents according to manufacturer's instructions.