title: Metatranscriptomic RNA sequencing of bronchoalveolar lavage fluid from a pediatric patient
design: 200uL of sample was combined with 200uL Zymo RNA Shield and underwent mechanical homogenization with 0.5mm glass bashing beads.  RNA was extracted with a Zymo column-based kit and DNase.  Sequencing libraries were created with random primers using a New England Biolabs kit.