title: GSM8122661: SalContrl_3; Salmonella enterica subsp. enterica serovar Newport; RNA-Seq
library_construction_protocol: Total RNA was extracted from each sample using the RNeasy® Mini Kit and rRNA removal . The mRNA was isolated from the total RNA was fragmented into short segments using a fragmentation buffer. cDNA was synthesized via reverse transcription (Superscript III, Invitrogen). The fragmented mRNA was used to first-strand cDNA using random primers, and the second-strand cDNA was synthesized with dUTP instead of dTTP. The synthesized cDNA was subjected to end-repair and 3' adenylation. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments. The U-labeled second-strand template was digested with Uracil-DNA-Glycosylase (UDG) before PCR amplification. The library was amplified to make DNA nanoball (DNB), and the sequencing was performed on the DNBSEQ (DNBSEQ Technology) platform.