title: GSM8195975: ESCC2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the tissue using Trizol reagent (Invitrogen, Carlsbad) according to the manufacturer's protocol.1-2 ug of total RNA was used for the construction of sequencing libraries. The cDNA library was constructed using KAPA Stranded RNA-Seq Library Preparation Kit (Illumina) following the manufacturer's protocol