title: Whole Genome Sequence of P. falciparum NF54 piggyBac mutant
design: Genomic DNA was harvested from cell cultures of the NF54 parent clone or PB52 mutant via saponin lysis of red blood cells and DNA extracted via the Qiagen QIAamp DNA Blood Mini Kit, with DNA concentration determined via Qubit 4 Fluorometer. The NEBNext Ultra II DNA Library Preparation Kit was used to prepare samples for sequencing. Samples were sequenced using NextSeq 550 at 300 cycles.