title: GSM7350035: 734hcp MOUSE 11; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using Nucleo Spin TriPrep kit (Macherey-Nagel GmbH & Co.KG, Germany) according to manufacturer's instructions. RNA was used as input for PolyA+ directional RNA-seq library preparation using the NEBNext Ultra II Directional RNA-seq Kit (#E7765, NEB) with the PolyA mRNA magnetic isolation module (#E7490, NEB) according to the manufacturers instructions. The pooled library was sequenced on an Illumina HiSeq 2000 with a 2x100 kit.