title: GSM8372477: Unexposed, CD13+EGFP-, donor 0620; Homo sapiens; RNA-Seq
library_construction_protocol: Transwell cultures were washed with DPBS and digested with TrypLE (Thermo Fisher Scientific) for 15 minutes, achieving single-cell suspensions through repeated pipetting. The cells were resuspended in culture medium supplemented with Y-27632 dihydrochloride (10 µM, Abmole Bioscience) and stained with CD13-APC antibody (1:250, BioLegend) for 20 minutes at 4 °C. Subsequently, the cells were washed, stained with DAPI (Sigma-Aldrich), and filtered through a cell strainer (Falcon) for sorting.Cells were sorted into 500 µL of TRIzol (Thermo Fisher Scientific) using different gating settings. A range of 25,000 to 150,000 cells were collected and subjected to RNA isolation. For library preparation of bulk RNA sequencing, a minimum of 100 ng of total RNA per condition was utilized. Library construction was performed by USEQ (Utrecht, the Netherlands) using a TruSeq Stranded mRNA library kit