title: RNA-seq of monoculture of V. dispar during the log phase, B2
design: Bacteria, V. dispar and L. acidophilus, were harvested during specific growth phases and then centrifuged. Total RNA was extracted using the Presto mini RNA bacteria kit. RNA concentration was measured with a Qubit fluorometer, while its purity and integrity were verified using a NanoPhotometer and an Agilent FA5200. For RNA sequencing, cDNA libraries were made using the Illumina Ribo-Zero Plus kit and sequenced on a Novaseq 6000 platform by Genomics Co. in Taiwan. The data was cleaned using a Phred score threshold of 30.