title: GSM8295964: HT29 cells, La-RIPK3 expression, light, RIPK3 kinase inhibitor, biological replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA of each sample and replicate was extracted using the RNA purification kit (Thermofisher) and treated with DNase (Promega) for 1 h in a 37 °C incubator to degradation of genomic DNA, followed by re-purification of RNA. Finally, the integrity of each RNA sample (300 ng) was assayed through 1% Bleach agarose gel with ethidium bromide. 100-300ng of RNA was submitted for construction of the sequencing libraries. RNA libraries for RNA-seq were underdone quality check using AATI Fragment Analyzer, followed by PolyA-selected mRNA library construction and MiSeq Titration.