title: GSM8073398: DCs_GC5; Homo sapiens; RNA-Seq
library_construction_protocol: RNA samples were isolated using RNeasy Plus Mini Kit (Qiagen). For scRNA-seq, the process began with sorting samples into PBS-0.04% BSA solution, which were then loaded into Chromium microfluidic chips. The chips utilized 10X Genomics' 5′ v2 chemistry to create gel-bead emulsions in a Chromium controller, targeting around 10,000 emulsions with unique cell barcodes. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount. Single-cell RNA sequencing libraries were employed the Chromium Next GEM Single Cell 5' Kit v2, along with other specialized kits and guides provided by 10X Genomics.