title: GSM7968140: OGD 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted from BV2 cells using TRIzol Reagent (Invitrogen, cat. NO 15596026.DNA digestion was carried out after RNA extraction by DNaseI. RNA quality was determined by examining A260/A280 with Nanodrop OneCspectrophotometer (Thermo Fisher Scientific Inc). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qubit3.0 was used to quantify qualified RNAs using the QubitTM RNA Broad Range Assay kit(Life Technologies, Q10210). A total of 2ug RNAs were used for stranded RNA sequencing library preparation using KCTM Stranded mRNA Library Prep Kit for Illumina (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China). PCR products corresponding to 200-500 bps were enriched, quantified and finally sequenced on Novaseq 6000 sequencer (Illumina) with PE150 model.