title: WGS of Pratylenchus vulnus
design: DNA isolation was performed using QIAampDNA Micro kit (Qiagen, Hilden, Germany) following the manufacturers protocol. Total genomic DNA was sheared for 50 s using a Diagenode Bioruptor Pico (Diagenode, Inc., Denville, NJ, USA) to obtain peak library fragment sizes of ~500 bp, and genomic libraries were prepared using the NEBNext_Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) following the manufacturers instructions.