title: GSM8526702: MH-S cells LPS treated replicate1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from tissues using TRlzol (lnvitrogen, Carlsbad, California, USA) according to manual instruction.The TRlzol was directly added to  the cell sample  with the amount 1 ml per 1 x 106 cells, followed by vortex mixing and standing for  5-minute. Library preparation is performed using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen, China). A certain amount of RNA are denatured at suitable temperature to open the secondary structure, and mRNA is enriched by oligo (dT) attached magnetic beads.