title: GSM7795489: sgZ8-EZH2,ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated for ChIP-seq. Chromatin Immunoprecipitation was performed with GenSeq® Chromatin Immunoprecipitation (ChIP) Kit (GenSeq Inc.) according to the manufacturer's instructions. The yield of ChIPed DNA was determined via Quant IT fluorescence assay (ThermoFisher), and sequencing libraries were generated with GenSeq® Rapid DNA Library Prep Kit (GenSeq Inc.) by following the manufacturer's manual. The library quality was determined by using Agilent 2100 Bioanalyzer (Agilent), and then, subjected to high-throughput 150 base paired-end sequencing on Illumina NovaSeq sequencer.