title: GSM8073575: macrophages, R1_1h_Mock; Mus musculus; RNA-Seq
library_construction_protocol: Trizol Total RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for all samples was ≥5.0. DNA libraries suitable for sequencing were prepared from 500 ng of total RNA using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero™ Plus (Illumina) according to manufacturer's instructions. After 14 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~330 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips. Dual RNA-seq