title: GSM8213923: Bone-KO-4; Mus musculus; RNA-Seq
library_construction_protocol: Femurs were isolated from 24-week-old male WT (n=4) and Girk3-/- (n=4) mice. The epiphyses were removed, then the diaphyseal bone was flushed with PBS three times to remove all bone marrow. Bone tissue was then placed in 1 mL trizol, homogenized, and total RNA was extracted using the Zymo Direct-zol RNA Miniprep Kit. RNA Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina per the manufacturer's instructions (NEB, Ipswich, MA).