title: GSM8164592: RNA_T12_NSD_T12N5_L; Mus musculus; RNA-Seq
library_construction_protocol: Mice were anesthetized with isoflurane prior to decapitation. Liver was rapidly dissected and flash frozen in liquid nitrogen. Frozen Liver of each individual was ground in liquid nitrogen and stored at -140°C until further use. Total RNA was extracted using the miRNeasy kit (Qiagen; Hilden, Germany) following the manufacturer's instructions. RNA-seq libraries were prepared using 10 ng/μl of total RNA and the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, CA, USA). Libraries were sequenced on the Illumina HiSeq 4000 sequencer, producing >24 million mappable single-end reads.