title: GSM6721391: CDKO1102_iMeLC_scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: TET1 CTRL and CDKO hESCs were differentiated as described above into iMeLCs and D4 aggregates for 10X Genomics analysis.  hESCs, iMeLCs and D4 aggregates from all six lines (n= 18 samples total) were dissociated into single cells with 0.05% Trypsin-EDTA (GIBCO, 25300-054), strained with 40um cell strainer (Flacon, 352340), and washed 5 times with ice-cold DPBS with 0.04% BSA.  Cell suspensions with viability over 70% was then proceeded to 10X Genomics GEM formation and library prep. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).