title: 16S metataxonomic sequencing of denture biofilm in pneumonia patient
design: Qiagen PuraGene extraction kits used according to manufacturer's instructions for gram-positive bacteria. Library preparation and sequencing was undertaken by Research and Testing Laboratories (RTL, Texas,168 USA). Preamplification was undertaken using Illiumina i5 and i7 primers, then NGS undertaken using Illumina Miseq 28f and 519r primers to overlap the V1  V3 hypervariable regions of169 the 16S rRNA gene.