title: RNA-seq of Acropora hyacinthus
design: *PROCESS AS PAIRED READS* Samples of nubbins were preserved in TRIzol solution (ThermoFisher Scientific) for the purpose of extracting total RNA. Ten samples in total (including one pre-acclimation sample and three biological duplicates from each experimental tank per period) were utilized for the extraction of total RNA, adhering to the TRIzol extraction protocol (ThermoFisher Scientific Inc., 2016). The total RNA samples were then dispatched to GenomeLead Inc. (Kagawa, Japan) for the sequencing process. The library was prepared using the Illumina Stranded mRNA Prep kit (Illumina), and sequencing was carried out on the Illumina NovaSeq 6000 platform. The FastQC v0.11.9 program (Andrews, 2010) was used to confirm the quality and length of the sequence reads. Trimmomatic (Bolger et al., 2014) was used to trim the Illumina adapters used for sequencing and any low-quality reads.