title: GSM8017908: Brain, 45H_G71_L40458_250; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from half-brain samples using the RNeasy Kit (Qiagen) Sequencing libraries were prepared with 250 ng of total RNA using Illumina Stranded Total RNA with Ribo-Zero Plus library preparation (Illumina Inc). PCR-enriched fragments were validated on a 2200 TapeStation (Agilent Technologies) and quantitated via qPCR. The final library was sequenced by 100 bp paired-end sequencing on a HiSeq 2500 (Illumina) at the Collaborative Sequencing Center (Translational Genomics Research Institute, Phoenix, AZ)