title: raw reads for HIV-RT error rate determination using single-strand consensus sequencing analysis: cellular RNA, 3 mM mg2+
design: First, RNA templates containing the HIV-1 protease (PR)-coding sequence were obtained from viral RNA produced by 8E5/LAV infected cells. The obtained RNA was reverse transcribed by an HIV-1 reverse transcriptase (M/B/BH10) using a primer with a Unique Molecular Identifier (UMI) or barcode of 14 nucleotides. Reverse transcription was carried out with 3 mM Mg2+ at 37C. The cDNAs obtained after reverse transcription were amplified by PCR and during this process adapter sequences were incorporated to facilitate subsequent NGS sequencing on an Illumina MiSeqTM Sequencing System.