title: Genomic sequence of Pantoea allii Eh250: Nanopore reads
design: High molecular weight genomic DNA was extracted (Miller et al. 1988) with the following modifications: the pre-lysozyme lysis buffer was 1M Tris pH 7.5, 0.5M EDTA pH 8.0, 5M NaCl, lysozyme digestion was performed at 48C for 1h, DNA resuspension was in 10 mM Tris pH 7.5, RNAse treatment was followed by two additional washes. DNA quantity was assessed using a Qubit 3.0 fluorometer (Thermo Fisher Scientific, U.S.A.). DNA integrity was assessed by agarose gel electrophoresis.  The isolated DNA was used to prepare a Nanopore sequencing library using the ONT Rapid Barcoding Sequencing kit (SQK-RBK004). The sequencing reaction was run in a MinION flow cell. Base calling was performed using Guppy v. 4.2.2 (<https://github.com/nanoporetech/pyguppyclient>).