title: DNA-Seq of unactivated 293T cell
design: Forty-eight hours post-transfection, cells were pelleted and washed with DPBS. Total RNA and DNA were extracted. DNA was amplified in 8  30 l reactions using F-mcherry-R-rd1 and R-V-Amp-1-rev1-UMI2-rd2 primers for three cycles (first-round PCR). The purified first-round PCR products were then amplified using P5-i5 (index)-rd1 and P7-i7 (index)-rd2 primers for fifteen PCR cycles to add P5, P7. Following SPRI cleanup, amplicons were sequenced using a NovaSeq 6000 SP flow cell with a 15% PhiX spike-in.