title: GSM6502179: MAB_Seq_n1_NSC_Astro; Mus musculus; Bisulfite-Seq
library_construction_protocol: Cells were sorted as follows: NSCs/astrocytes: Glast+/CD9high, oligodendrocytes: O4+, neuroblasts (PSA-NCAM+). For every biological replicate, 12 mice were pooled. Between 10,000 and 20,000 cells were sorted and processed following the MAB-seq strategy described in Wu et al. (2014). Specifically, depending on the sample, 20 ng to 90 ng DNA were used for four successive M.SssI (NEB) treatments to ensure maximal methylation. DNA was purified using HighPrep beads (Biozym) added in a 1:1 ratio (v/v). Four MAB-seq libraries per sample were generated and pooled according to the tagmentation-based whole genome bisulfite sequencing protocol for very low DNA input (PubMed ID: 30779037).