title: GSM8164530: SNU719, 4C-seq, siCtrl, rep 1; Homo sapiens; OTHER
library_construction_protocol: Cells were cross-linked with 1% formaldehyde for 10 min, quenched with 0.2 M of glycine, lysed with lysis buffer, and digested with primary restriction enzyme, DpnII. The digested DNA fragments were then subjected to ligation by using T4 DNA ligase (Roche). After digested with the second restriction enzyme, Csp6I, and circularization by T4 DNA ligase, the 4C template was amplified by a PCR reaction and purified with AMPure XP beads. Extracted 4C templates were amplified with primers, which consist sequence of the bait MYB promoter region. After 1st PCR, amplified DNA was extracted using Ampure XP beads, and 2nd PCR using primers consist NGS adaptor sequence and index was performed. After 2nd PCR, the amplified 4C-seq library was extracted using QIAGEN PCR purification kit following the manufacturer's protocol.