title: TCR Beta-SR Assay
design: Purified RNA was extracted from buffy coat. Rare or abundant clones canbe identified with as little as 10 ng RNA input, and this useof RNA template improves rare clone identification throughthe sequencing of productive and relevant variable (V),diversity (D), and joining (J) rearrangements. The analysis of human TCR beta chainCDR3 regions amplified with the Oncomine TCR Beta-SR assay was performed according to the manufacturer's instructions.