title: RNA-Seq of HT1080/Fucci(CA)5 fractionated according to Fucci expression by FACS, fraction f1
design: HT1080/Fucci(CA)5 cells were sorted into 96-well plates with 100 cells per well using a FACSAria into 7 fractions according to Fucci expression. After reverse transcription, cDNA was amplified by PCR and a cDNA library was prepared using Nextera XT DNA Library Preparation Kit.