title: GSM6974029: eCLIP,GFP_IP,bio_rep2; Homo sapiens; RIP-Seq
library_construction_protocol: For CRISPR screen, genomic DNA was extracted using the Zymo Research Quick-gDNA Midiprep Plus kit (D4075); For eCLIP, RNAs were extracted using the RBP-eCLIP kit (ECLIPSEbio) according to the manufacturer's instructions. For CRISPR screen, sgRNA sequences were amplified by PCR following the previously described procedure (Joung et al, 2017, Nat Protoc); For eCLIP, libraries were constructed using the RBP-eCLIP kit (ECLIPSEbio) according to the manufacturer's instructions.