title: GSM8494230: male bone marrow cells with high ALDH activity, rep2; Mus musculus; RNA-Seq
library_construction_protocol: The bone marrow cells obtained from male and female mice were suspended using 1 mL TRIzol reagent (Life Technologies) and total RNA was extracted using the RNeasy Mini Kit (Qiagen). Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.