title: RNA-seq of zebrafish liver 1 dpci
design: ham and injured livers at 1, 3, and 7 dpci were phenotyped under the fluorescent stereomicroscope (NSZ-606 Binocular Zoom fitted with a NightSea SFA light base) to confirm the presence of insult upon cryoinjury. In addition, 3 adult zebrafish livers were pooled per tube, discriminating between the injured border and liver tissue from other lobes. Finally, 3 replicates of 3 pooled livers were used for library preparation. Livers were transferred to a final volume of 300uL of cold TRIzolTM (Thermo Fisher Scientific) per tube on ice. Livers were homogenized using the mechanical homogenizer for 30s on ice, with a plastic pestle, to ensure fine homogenization. RNA was extracted according to the manufacturer guidelines (Direct-zolTM RNA MiniPrep kit, Zymo Research). RNA quality was confirmed using an Agilent 4200 Tapestation System. Libraries were sequenced in Illumina NextSeq 500, with paired-end 75bp reads to a depth of 15M reads per sample.