title: Microbiome shotgun of Mus musculus feces
design: Feces were collected from mice at ZT8 and homogenized with 1 ml of DNA/RNA shield (Zymo Research, Irvine, CA). Fecal samples were processed, sequenced, and analyzed by Zymo Research (Irvine, CA) with the ZymoBIOMICS Shotgun Metagenomic Sequencing Service for Microbiome Analysis. In brief, DNA was extracted using the ZymoBIOMICS-96 MagBead DNA Kit (Zymo Research, Irvine, CA). Sequencing libraries were prepared with the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA) using 100 ng DNA input and internal dual-index 8 bp barcodes with Nextera adapters (Illumina, San Diego, CA) according to the manufacturers protocol. All libraries were quantified with TapeStation (Agilent Technologies, Santa Clara, CA) and then pooled in equal abundance. The final pool was quantified using qPCR and the library was sequenced on the Illumina NovaSeq (San Diego, CA).