title: GSM6664598: MinusAP_EGFRGluGlu_BiolRep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 37% methanol-stabilized formaldehyde at a final concentration of 1% for 10 min at room temperature.  Crosslinking was quenched with 0.125 M Glycine for 5 min at room temperature.  Cells were lysed with 300 µl lysis buffer (50 mM Tris-HCl [pH 8.0], 1% SDS, 5 mM EDTA, 10 µg/ml aprotinin, 10 µg/ml leupeptin, 1 µg/ml pepstatin, 1 mM PMSF). Approximately 0.1–20 ng ChIP DNA was fragmented to a size range of 200 bp on a Covaris instrument for 2 mins using 15 µl microTUBE-15 AFA Beads Screw-cap.  Libraries were prepared with the NEBNext Ultra II DNA library preparation kit (New England Biolabs) and quantified by Qubit fluorimetry and quality controlled on a Bioanalyzer.