title: RNAseq of HEK293T_WT replicate 3
design: Total RNA was extracted and purified using a Qiagen RNeasy Plus kit (Qiagen, Cat# 74134) and further treated with DNase (Invitrogen, Cat# AM1907). The Illumina libraries were prepared from 500 ng of total RNA, using Illuminas TruSeq Stranded Total RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), according to the manufacturers protocol. The cDNA libraries were then checked on the Bioanalyzer 2100 and quantified by fluorimetry using the Quant-iTTM PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on NanoDrop 3300 Fluorospectrometer (Thermo Fisher Scientific). Sequencing was performed on a NovaSeq 6000 platform using the paired-end approach (2100 bp) with 262638 million reads per sample.