title: GSM7246812: NPC, INTS10HET2, replicate 1, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: To harvest cells for single-cell RNAseq, cultured NPCs were incubated with Accutase (A6964) for 5 min at 37 ℃. An equal volume of DPBS was added to each well to collect the cells. Cell suspension was strained through 40um Cell Strainer (XXXX) twice. The single cell suspension was centrifuged at 400 x g for 5 mins and resuspended in DPBS for counting. Cell viability was examined by 0.4% Trypan blue. Single cell suspension with 98% viability containing 15, 000 viable cells (aiming for recovery of 10, 000 cells) were processed using the Chromium Next GEM Single Cell 3' Kit v3.1 to generate cDNA libraries following manufacture's instructions. The quality of cDNA and libraries were assessed by the Agilent 2100 Bioanalyzer System ( or TapeStation). Libraries were sequenced on IllumniaXXXXX. Chromium Next GEM Single Cell 3' Kit v3.1