title: RNA-seq of K. phaffii: H2O2 treated
design: Yeast cells were shaking cultured at 220 rpm, 30 . For seed preparation, yeast cells were inoculated into YPD medium (2% tryptone, 1% yeast extract, 2% glucose) until OD600 reached 6-8. Cells were then inoculated into fresh YPD medium again with initial OD600=0.1 and shaking cultured until steady state.  For oxidative stress induction, H2O2 (final concentration 0.5 mM) was added into culture medium when cell density reached 8 OD/mL and cultured for another 8 hours. Yeast cells were collected by centrifugation and broken by tissue grounding instrument (YeTuo). Total RNA was extracted by TRIzol reagent. After quanlity control, sequencing library was constructed with NEBNext UltraExpress RNA Library Prep Kit and sequenced by Illumina system (Genewiz).