title: GSM8296230: Primed-ESC_1_DJ_IR; Homo sapiens; OTHER
library_construction_protocol: To isolate 3C DNA, cells were thawed and gently lysed on ice for 30 minutes to release nuclei. Nuclei were washed and permeabilized by incubating in 1% SDS and 1.15X NEB3.1 buffer for 1 h, and then in the presence of 2.85% Triton-X100 for 1 h. Following digestion and re-ligation protcols, DNA was purified using standard phenol-chloroform and ethanol extraction. Library amplification was carried out according to the UMI-4C protocol (Schwartzman et al. 2016).