title: Cherry site 1 Plant tissue 2
design: total RNA (totRNA) was extracted from each bee-related sample using the spectrum total plant RNA extraction kit (Sigma Aldrich, ON, Canada) while double stranded RNA (dsRNA) was extracted from plant samples. dsRNA was extracted from leaf and flower samples following Kesanakurti et al. (2016). Extracted totRNA was treated with a rRNA depletion step using the RiboMinusTM Plant Kit for RNA-Seq (Invitrogen, MA, USA) as per the manufacturers instructions. Ribo depleted totRNA and dsRNA HTS libraries were generated using the Illumina TruSeq Stranded mRNA Library Prep kit, following the manufacturers protocol, starting after mRNA selection steps (Kesanakurti et al., 2016). An Illumina NextSeq500 was used to generate single-ended 75 base read files to each sample.