title: GSM8261983: 890-CNTNAP2-KO-P30-M_S30_L002; Mus; RNA-Seq
library_construction_protocol: Brain samples used for flow cytometry were homogenized with pestles immediately after dissections and single-cell suspension were generated using Falcon 70 um cell strainer (Corning). After the isolation, brain cells were washed with complete medium and incubated for 12h at 37°C. Total RNAs were extracted from cerebral cerebella of adult Cntnap2 +/+  Cntnap2-/- mice using RNeasy Plus Mini Kit (Qiagen), and retro-transcribed to cDNA according to published protocol. The quality and the integrity of the RNA samples, RNA Integrity Numbers (RIN) were assessed using a 2100 Bioanalyzer Instrument (Agilent). Only samples with RIN > 8 were considered for the analysis. Libraries were prepared using a TruSeq Stranded mRNA Library Prep (48 Samples) Kit (Illumina). Library preparation, quality check, and sequencing were carried out using Illumina NovaSeq6000.