title: GSM8423455: cortex,WT,OLIG2,P1-2, CUT&Tag; Mus musculus; OTHER
library_construction_protocol: ScRNA-seq were performed on FACS sorted tdT+ cells from the P1 Olig1/2f/f;IS-reporter mice cortex which were electroporated of pCAG-Cre plasmid at E15.5.Emx1-CRE;Olig1/2f/f mice cortex were dissected out at P7 and immediately submerged in fresh ice-cold Hanks balanced salt solution. The cortex was then cut into pieces and dissociated into a single-cell suspension using a Papain Cell Dissociation Kit according to the manufacturer's instructions. The CUT&Tag assay was conducted on FACS-sorted GFP+ cells, using the cortex dissected from hGFAP-GFP at P1 The Chromium dropletbased sequencing platform (10X Genomics) was used to generate scRNA-Seq libraries, following the manufacturer's instructions (manual document part number: CG00052 Rev C). The cDNA libraries were purified, quantified using an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 or Illumina Hiseq4000. CUT&Tag liabraries were prepared using the Vazyme#TD901 complemented with TruePrep® Index Kit V2 for Illumina  10 ng of DNA was used as starting material for CUT&Tag simples.Liabraries were amplified using 18 cycles on the thermocycler.