title: WGS of Xiphophorus cortezi x Xiphophorus birchmanni: caudal fin
design: We collected whole genome resequencing data following the library preparation of Quail et al. (2009). Between 700 ng and 1 ug of DNA was sheared using a QSonica sonicator to an average of 400bp. Sheared DNA was end- repaired with a mastermix of T4 DNA polymerase, T4 PNK, Klenow DNA polymerase, and dNTPs by incubating at room temperature for 30 minutes. The end-repaired DNA was A-tailed by incubating at 37C for 30 minutes with a mastermix containing dATPs and Klenow exonuclease. After A-tailing, universal adapters were ligated with DNA ligase for 15 minutes at room temperature, and the library was purified with the Qiagen QIAquick PCR purification kit. We performed a final PCR amplification with indexed primers (12 cycles) and purified the PCR product with 18% SPRI beads.