title: GSM6721739: CD45, nuclease, presort, Cas9NG; Homo sapiens; OTHER
library_construction_protocol: gDNA was extracted using GeneJET DNA Purification Kit (Thermo Fisher Scientific). gDNA was  used in the first PCR reaction, in multiple reactions not exceeding 10 μg gDNA per 100 uL PCR  reaction. After the first PCR to process gDNA, the samples were  subjected to a second PCR to add barcodes and sequencing adaptors. PCR products were run on a 2% agarose gel to extract the correct size band. PCR products from different samples were then pooled together in  equimolar ratios. Sequencing was performed on the NextSeq 500 instrument using the MidOutput Mode  v2 with 75 bp paired-end reads (Illumina).