title: Metagenome of gut microbiome: human stool
design: V3V4 hypervariable region of the 16S rRNA gene was amplified with primers 341F and 805R using the direct polymerase chain reaction method. NEBNext Ultra II FS DNA Library Prep Kit for Illumina was used to construct DNA libraries. Sequencing of prepared DNA libraries was conducted using the Illumina MiSeq platform with 2317 base pair kits