title: GSM8018717: Mouse brain, microglia,EAE, replicate3; Mus musculus; RNA-Seq
library_construction_protocol: Microglia (CD11b+/CD45int) were purified. Then, total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion). Ribosome RNA was then digested using the TruSeq Stranded. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructed using TruSeq Stranded Total RNA with Ribo-Zero Gold according to the manufacturer's instructions.