title: Genomic sequences of the sugar beet root maggot Tetanops myopaeformis, TmSBRM_v1.0
design: DNA isolation and sequencing was performed at CD Genomics using their proprietary methods. Briefly, DNA was isolated from liquid nitrogen flash frozen larvae. For sample preparation and DNA quality control, isolated DNA quality and quantity were assessed using Qubit and Agilent 5200 Fragment Analyzer. For DNA fragmentation, the DNA is cut into 15 Kb or larger fragments using the Covanis g-TUBE, and subsequently purified using magnetic beads. For DNA repair and end modification, DNA repair enzyme is used to correct any DNA damage and ensure uniformity. Furthermore, the overhang adapters are ligated to the end of the repaired DNA ends, followed by purification using magnetic beads. For SMRTbell library preparation, the repaired and adapter-ligated DNA fragments are then converted into SMRTbell libraries. For SMRTbell library size selection, the SMRTbell libraries are subjected to BluePippin size selection to enrich the fragments over 9-13 Kb and 15 Kb. For binding to polymerase, the size-selected SMRTbell libraries are bound to DNA polymerase molecules. For DNA sequencing, the prepared SMRTbell libraries, with bound polymerase molecules, are loaded onto the PacBio Sequel Revio sequencing platform with options enabled to retain subreads and kinetics sequencing metrics.