title: GSM7246690: Day 5 infection non-Tfh-2 (Th1-2) [ATAC-seq]; Mus musculus; ATAC-seq
library_construction_protocol: Briefly, the sorted cell pellets were washed and treated in lysis buffer for 3 min on ice. The extracted nuclei were resuspended in transposition mix containing 2.5 μl Transposase (Illumina) and incubated at 37oC for 30 min. The products are purified with MinElute Reaction Cleanup Kit (Qiagen), and then amplified by PCR for 13 cycles with barcoded Nextera primers (Illumina) DNA fragments in the range of 150-1,000 bp are recovered from 2% E-Gel EX Agarose Gels (Invitrogen/ThermoFisher Scientific)