title: GSM6661663: mouse prostate and urethra spatial expression sample 2 replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Frozen sections of urethra and connecting prostate tissues from C57BL/6 mice were fixed for H&E staining and imaging following the manufacturer's instruction (CG000160, 10X Genomics, Pleasanton, CA). Tissue permeabilization was performed for 12 min and spatially barcoded full-length cDNA was generated using Visium Spatial Gene Expression Slide  & Reagent Kit (PN-1000187, 10X Genomics, Pleasanton, CA). Library construction was performed according the the manufacturer protocol Visium Spatial Gene Expression Slide  & Reagent Kit (PN-1000187, 10X Genomics, Pleasanton, CA). CDNA amplification was conducted with 14 cycles of PCR reaction and 10 µl cDNA was used for the library preparation.