title: GSM8372210: fatbody_OA_rep1; Bombus terrestris; RNA-Seq
library_construction_protocol: B. terrestris workers were quickly dissected on ice to minimize RNA degradation, with the brains and ovary being stored in RNAlater (Sigma-Aldrich) and the fat body being stored in AllProtect (Qiagen). Tissue from seven individuals were pooled together to make a sample. Samples were ground in liquid nitrogen using a 2 ml pestle (Eppendorf) and the powder resuspended in Tri-Reagent (Ambion). The RNA was then extracted using a Direct-zolTM RNA extraction kit (Zymo Research), including an on-column DNase treatment. The eluted RNA was further treated with TurboTM DNase (Ambion). The same protocol was used for preparation of RNA from eggs. Standard mRNA-seq was conducted for the brain, fat body and ovary samples by Edinburgh Genomics (The University of Edinburgh, Edinburgh, UK) on an Illumina HiSeq 2500 sequences using Illumina TruSeq adapters. The two egg mRNA-seq libraries were constructed and sequenced by Baseclear (Leiden, The Netherlands) using an Illumina HiSeq 2500 sequences with Illumina TruSeq adapters.