title: GSM8001970: PJI TNF KO mice 3; Mus musculus; RNA-Seq
library_construction_protocol: CD45+Ly6G+ Granulocytes were isolated from WT and TNF KO C57BL/6 mice 7 days following craniotomy or prosthetic joint infection. Granulocytes from 3 mice were pooled into each sample, lysed, and RNA isolated via Quick-RNA Microprep Kit with target of 500 ng total RNA. RNA libraries were prepared using 50 ng of input RNA and the Nugen Universal mRNA Library Prep Kit according to manufacturer instructions. Sequencing was performed on a NextSeq500 (Illumina) with 150 mid-output cycles (75 paired-end reads) at a sequencing depth of ~10 million paired reads per sample.