title: GSM6585527: ATAC DE 12h rep 2; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq: 50K cryopreserved cells were washed in cold PBS and lysed. The transposition reaction containing TDE1 Tagment DNA Enzyme (Illumina; 20034198) was incubated at 37°C for 30 minutes. The DNA was purified with the MinElute PCR Purification Kit (QIAGEN; 28004) and  amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; 529 M0541L). After evaluation by real-time PCR, 3-14 additional PCR cycles were done. Final product was cleaned by AMPure XP beads (Beckman Coulter; A63882) at a 1X ratio, and size selection was performed at a 0.5X ratio. ATAC-seq: Libraries were sequenced on a HiSeq 4000 or NovaSeq 6000 platform in a PE50 run, using the HiSeq 3000/4000 SBS Kit or NovaSeq 6000 S1 Reagent Kit (100 Cycles) (Illumina).