title: GSM8371989: Prdm1-KO; Mus musculus; RNA-Seq
library_construction_protocol: FACS-sorted liver CD45+ cells with more than 80% cell viability were used for library preparation. Each sample contained cells from three Prdm1+/+ or Prdm1ΔNcr1 mice. Library was performed  according to manufacturer's instructions. Briefly, Gel Bead-in-Emulsions (GEMs) were generated using the 10X Genomics Chromium system, which combinates Master Mix, Single Cell 3' v3.1 Gel Beads, and Partitioning Oil with single cells. GEMs were mixed with cell lysate and reverse transcription reagent to produce full-length cDNA. After incubation, the GEMs were broken, and recovered cDNA were amplified via PCR. Fragmentation, End repair, A-tailing, and Adaptor Ligation were performed to obtain final libraries, which contain P5 and P7 sequences. The 3' library was sequenced on Novaseq 6000 with approximately 50k read pairs/cell sequencing depth.