title: GSM8073630: Vector; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell suspensions from lung tissue were collected. The cells were labeled with an anti-human-CD3-PE/Cy7 antibody and a live/dead dye. FACS-sorted cells were gated on CD3+ live cells. Sorted cells from 5 mice were combined and were washed with PBS, and cell numbers and viabilities were assessed by trypan blue staining. The 10,000 CD3+ T cells isolated from the lung were used for scRNA-seq library prep. scRNA-seq library prep was  following the protocol from 10x Genomics Chromium Next GEM Single Cell 5' Reagent Kits V2.