title: GSM7745147: Liver_CCL4.siHAS2_ rep1; Mus musculus; RNA-Seq
library_construction_protocol: PolyA mRNA was isolated from liver samples using Trizol(15596018, Thermo Fisher Scientific), following manufacturer's protocol A measure of 1 μg RNA was reverse-transcribed to cDNA using the QuantiTect Reverse Transcription Kit (Qiagen, Hilden, Germany). Illumina cDNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, MA, USA) following the manufacturer's protocol. cDNA libraries were sequenced using the NextSeq500 (Illumina, San Diego, CA USA) instrument. Sequencing was carried out with a read length of at least 85bp in the paired-end mode, with an average number of reads per sample not less than 30 million