title: RNASeq of Grubraw:Grubraw_D-ala-YES_replicate-2
design: RNA was extracted according to manufacturers instructions by Qiagen RNeasy Mini Kit (Qiagen). For library construction we used NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) according to manufacturers instruction. Sequenced on NovaSeq6000 (Illumina) with pair-end 61 bp reading.