title: RNA-seq of Euprymna scolopes hatchlings (whole organism)
design: Libraries were prepared using the KAPA mRNA HyperPrep Kit; 100ng of total RNA was used, Poly-A containing mRNA was collected using poly-T oligo attached magnetic beads, followewed by their fragmentation to 200  300bp (94C for 6 min). Fragmented mRNA used as template to synthesize the first-strand cDNA by using random hexamer-primer and reverse transcriptase. In the second strand cDNA synthesis, the mRNA template was removed and a replacement strand was generated to form the blunt-end double-stranded (ds) cDNA. The ds cDNA underwent 3 adenylation and indexed adaptor ligation. The adaptor-ligated libraries were enriched by 15 cycles of polymerase chain reaction (PCR).