title: GSM7745448: miR-eCLIP Ago2IP with miR181a/b-1-KO sample2; Mus musculus; RIP-Seq
library_construction_protocol: Total thymocytes were extracted from thymy using 70µm strainers.  Isolated cells were then crosslinked using a using 254nm UV-C Mercury bulb in the (Analytik Jena CL-1000M). Cells were frozen and sent to Eclipse Bioinnovations (San Diego, 92121 CA, USA). Library construction was performed by Eclise Bioinnovations. No modifications were made to the published eCLIP methodology for this experiment. 20ug of total RNA was used per sample. Sequencing was performed as PE150 on the NextSeq platform.