title: GSM7113706: 4_M_CSF_IL_4_ IL_13; Homo sapiens; RNA-Seq
library_construction_protocol: To extract RNA, cells were harvested, washed with PBS and lysed in RLT Plus Buffer (Qiagen) followed by extraction of RNA using Rneasy® Mini Kit (Qiagen), according to the manufacturers' protocol. Extracted RNA samples were quantified by NanoDrop™ 8000 Spectrophotometer (Thermo Fisher Scientific) or NanoPhotometer® N120 (Implen). For sequencing library preparation, 100ng of total RNA was used as input for the TrueSeq RNA Sample Prep Kit v2-Set B (RS-122–2002, Illumina Inc, San Diego, CA) producing a 275 bp fragment including adapters in average size. Eight libraries were normalized and pooled together on a single lane and sequenced as 52 bp, single reads and 7 bases index read on an Illumina HiSeq2000 instrument using the TruSeq SBS Kit HS- v3 (50-cycle) (FC-401–3002, Illumina Inc, San Diego, CA).