title: RNA-Seq of mus musculus: lacrimal gland
design: We extracted LGs from 3 ND and 3 HFD mice. Total RNA isolation employed Trizol, and RNA quantification utilized Qubit Fluorometer (Invitrogen, Carlsbad, CA, USA) and Agilent 2100 BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA). The NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina was utilized to create sequencing libraries. Then, polymerase chain reaction (PCR) was employed for amplification, following which the subsequent cDNA library underwent sequencing with the Illumina Novaseq6000 from Gene Denovo Biotechnology Co. (Guangzhou, China).