title: GSM7113410: PrE3; Homo sapiens; miRNA-Seq
library_construction_protocol: Primary epithelial cell, stromal cell, and tissue slice RNA was harvested using miRNeasy  Mini kit (Qiagen). Primary epithelial cell EV, stromal cell EV, tissue slice EV, and serum EV RNA was harvested using the Serum/Plasma miRNeasy kit (Qiagen). 100 ng of total RNA was used for the construction of cell and tissue sequencing libraries. 5 uL of RNA was used for  the construction of cell EV, tissue EV, and serum EV sequencing libraries. microRNA libraries for smallRNA-seq were prepared using the QIAseq miRNA Library Kit following manufacturer's protocols. Libraries were diluted to 10 nM for sequencing. smallRNA-seq by HiSeq 4000 single read 100 nucleotides