title: GSM6585574: ChIP-seq H3K27ac ESC-0h biol rep 2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq: Cells were crosslinked with 1% formaldehyde and then lysed and sonicated. Clear supernatant was collected for chromatin immunoprecipitation, decrosslinking and DNA purification. Sequencing library was generated by using the NEBNext® Ultra II DNA Library Prep Kit (New England Biolabs; E7103S) and NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Index Primers Set 1; NEB, E7335S). ChIP-seq: Samples were pooled and sequenced on Illumina HiSeq 4000 platform.