title: GSM8527245: arterial (CD200-Sca1+CD31+CD45-) replicate1 sample; Mus musculus; RNA-Seq
library_construction_protocol: BM cells were obtained by crushing bone (tibia, femur, and iliac) using molars and pestles. RNA was isolated from sorted cells lysed in TRIzol. Biological triplicates were prepared using six B6 mice. RNA quality was assessed using Agilent Bioanalyzer RNA-Pico kit (Agilent Technologies, USA). Libraries were prepared using the Clontech Ultra Low v4 kit (Takara Bio) for cDNA synthesis, followed by NexteraXT (Illumina, USA), and sequenced on an Illumina NovaSeq6000