title: GSM8372032: FL_48_4885_rep1; Phaseolus vulgaris; RNA-Seq
library_construction_protocol: The collected leaves were ground to a fine powder after inoculation. Total RNA was then extracted from the ground leaves using the Macherey-Nagel NucleoSpin® RNA plus kit (Macherey-Nagel, Düren, Germany). RNA quality was assessed with a NanoDrop spectrophotometer (Thermo Scientific™ NanoDrop™ One). Samples with acceptable quality (260/280 and 260/230 absorbance ratios of 2) were sent to the Beijing Genomics Institute (BGI, Hong Kong)(https://www.bgi.com) for further quality assessment using a Bio Analyzer. The RNA Integrity Number (RIN) of the 36 samples averaged 7.32, and the 28S/18S ratio averaged 1.8. Library preparation and sequencing were conducted on the BGISEQ-500 platform, generating an average of 24 million reads of 100 base pairs per sample (24M PE100).