title: GSM8074090: quadriceps TG, CNO, clamped_8; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using RNeasy Lipid Tissue Mini Kit (perigonadal WAT) and RNeasy Fibrous Tissue Midi Kit with Proteinase K digestion (whole m. quadriceps from clamped mice), according to Qiagen's instructions. On-column DNase digestion using RNAse-Free DNAse Set (Qiagen) was applied. Libraries were generated using the QuantSeq 3' mRNA-Seq Library Prep kit from Lexogen (FWD), purified with the Purification module kit from Lexogen, indexed with the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1), and Lexogen UDI 12 nt Unique Dual Indexing Add-on Kits: A2 (UDI 12A 0097-0192, Cat. No. 119.384, Lexogen)(quadriceps clamped and pgWAT CNO 1h), or using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, 015UG009V0260) following standard procedures (as outlined in the user guide: https://www.lexogen.com/wp-content/uploads/2021/08/015UG009V0260_QuantSeq_Illumina_2021-08-16.pdf) (pgWAT CNO 6h).