title: PacBio high-fidelity (HiFi) sequencing of D21
design: High-molecular-weight genomic DNA (gDNA) was extracted from young leaves using an improved CTAB method, and then purified by Monarch Genomic DNA Kit (NEB, T3060). For long reads sequencing, 22-kilobase SMRTbell libraries were constructed according to the standard protocol of Pacific Biosciences (PacBio) and sequenced on the PacBio Sequel II platform to generate subreads in the circular consensus sequencing (CCS) mode.