title: GbS of Vitellaria paradoxa : Orchard, Mayongo, Logone-oriental, Chad
design: A total of 20 populations in three countries were identified as representative of the shea distribution gradient (Tab 1). In each population, individuals from mature (dbh > 30 cm), juvenile (dbh 10-30 cm), and regenerating (dbh < 10 cm) trees (Kelly et al., 2004), were sampled from the different land-use systems. In each population, 25-30 trees were sampled, and three to four dried leaves were collected from each individual tree. A distance of at least 50 m was maintained between individuals to avoid relatedness. Multiplex files were processed with cutadapt and demultiplexed with STACKS process_radtags.