title: TnPuro mutagenized Hap1 pool 3 - 4x FACS selected as P. aeruginosa T3SS resistant
design: DNA was extracted from 5*10^6 cells using a Qiagen DNeasy Blood & Tissue Kit. The DNA was cleaved using BfaI and CviQI (separately, then mixed together). A barcoded P7 linker was ligated to the fragmented DNA, followed by two rounds of amplification. Round 1, using a linker and a transposon-specific primer, round two reamplifying these PCR products and adding partial illumina primers for AmpEZ sequencing with Azenta