title: mda-amplified WGS of Amphiascoides atopus
design: genomic DNA was isolated using Zymo quick RNA kit, and eluted in 25 l of pure water, under strict conditions of environmental DNA decontamination (UV light; HEPA filtration chamber). The yield of the DNA was below the limits of detection with Qubit HS DNA kit. 2 l were submitted to multiple-displacement amplification with Repli-G (Qiagen), and the obtained DNA was submitted to library construction using the Ligation Sequencing Kit (SQK-LSK109) from Oxford Nanopore and sequenced in a dedicated FLO-MIN106D flowcell for 24hr.