title: mRNAseq of D. rerio: zWT
design: Biological replicates were bred from zbtb48+/- parents through single mating. RNA was extracted from 5 dpf larvae using AllPrep DNA/RNA/Protein Mini Kit (Qiagen) according to the manufacturers instructions.  NGS library preparation was performed with Illumina's TruSeq Stranded mRNA LT Sample Prep Kit following the TruSeq Stranded mRNA Reference Guide (Oct. 2017) (Document # 1000000040498v00) using  of the reagents.