title: RNA-Seq of B burgdorferi wild-type and badR mutant
design: Samples were treated with TURBO DNase (Thermo Fisher Scientific) to remove DNA contaminants and rRNA depletion was performed using QIAGEN FastSelect5S/16S/23S Kit (Qiagen). RNA sequencing libraries were prepared by using NEB Next Ultra II RNA Library Preparation Kit for Illumina following the manufacturers recommendations (New England Biolabs). Sequencing libraries were validated using the Tapestation 4200 system and quantified using Qubit 2.0 Fluorometer as well as by qPCR.