title: Mitochondrial DNA sequencing of homo sapiens: Cisplatin-resistant cells derived from OSCC SAS cell line (Amplicon)
design: Both nuclear and mitochondrial DNA (mtDNA) were extracted using a QIAprep Miniprep Kit (QIAGEN, Germany). The mtDNA fraction was enriched and size-selected using solid-phase reversible immobilization (SPRI) paramagnetic beads (Agencourt AMPure XP; Beckman Coulter Inc., USA). Two long PCR products, which spanned the entire mtDNA, were amplified using AtMax Taq DNA Polymerase (Vivantis Technologies, Malaysia) on an Arktik Thermal Cycler (Thermo Scientific Inc., USA). The DNA libraries were prepared using the 1D2 Ligation Sequencing Kit (SQK-LSK308; Oxford Nanopore Technologies, UK).