title: GSM8214458: shCon_TGFb_1; Homo sapiens; RNA-Seq
library_construction_protocol: The  total RNA  of the cells was isolated by TRIzol® Reagent (Invitrogen) then the construction of cDNA library was performed by Illumina TruSeq Small RNA sample prep kit. The target PCR products were purified through 6% Novex TBE PAGE gel and the quality was checked by TBS380 (Picogreen). Then PCR amplification was performed and the generated clusters were sequenced by Illumina HiSeq platform.