title: GSM8035909: Sample 18-75-L004 [snRNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: snRNA-seq libraries were prepared from nuclei using the Chromium Next GEM Single Cell 3ʹ v.3.1 according to the manufacturer's protocol (10x Genomics). Nuclei were counted using with a TC20 Automated Cell Counter (Bio-Rad) and loaded into a 10x droplet generator at a concentration of 8,000 nuclei per sample. Thirteen pcr cycles were applied to generate cDNA, and 15 cycles for final library generation. The final snRNA-seq libraries were sequenced on a NovaSeq 6000.