title: GSM8371846: YAP KO, RA-, Day 5; Homo sapiens; ATAC-seq
library_construction_protocol: Nuclei were isolated and permeabilized using OMNI buffer (0.1% NP40, 0.1% Tween-20, 0.01% Digitonin; Corces et al., Nat. methods 2017). Nuclei were counted and 2k nuclei were dispensed per well; 96 wells per sample for tagmentation. After tagmentation nuclei from individual samples were pooled. 20 nuclei from each sample were sorted per well (768 wells total; 2n/4n mix in 672 wells (plates 1-7) and 48 wells each for 2n and 4n, respectively (plate 8). Method adapted from: Cusanovich et al., Science, 2015