title: GSM8074043: pDCs_LAL-16h_rep3_R15; Mus musculus; RNA-Seq
library_construction_protocol: Harvest of the cells have been done at indicated time points (0h, 6h and 16h). After harvest, pDCs were put in QIAzolTM Lysis Reagent (QIAGEN) and further processed for RNA extraction with miRNeasy Micro Kit according to the manufacturer's instructions (QIAGEN). Samples were then further purified before downstream application with RNeasy® MinElute® Cleanup Kit to eliminate traces of contaminants, according to the manufacturer's instructions (QUIAGEN). cDNA synthesis was performed with SuperScript First-Strand Synthesis SystemTM (Invitrogen) according to the manufacturer's instructions. Sequencing libraries were prepared using the NEBNext rRNA depletion kit (human/mouse/rat) and the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina according to manufacturer's protocols (New England Biolabs). Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries were sequenced on a NextSeq500 instrument (Illumina) in 2x75bp paired-end sequencing mode.