title: GSM6925403: LSD1_HCT116_KO_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were used to extract the total RNA using the Total RNA Extraction Kit (Omega). In brief, a total of 1ug RNA﻿ per sample was used as input material, and then sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. All libraries were constructed using the TruSeq SBS v3-HS kit before sequencing to investigate the global transcriptome of the control group using Illumina HiSeq 2500 (Illumina, Inc.).