title: GSM6970349: proliferating 3h TGFB low R1 2018; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from the cells using the High Pure RNA Isolation Kit by Roche according to the manufacturer's instruction. TruSeq stranded mRNA LT Sample Prep Kit (Illumina) was used according to the manufacturer's instructions. Libraries were prepared with a starting amount of 500 ng RNA, amplified in 11 PCR cycles, and profiled using a DNA 1000 kit and a 2100 Bioanalyzer (both Agilent) Afterwards, the libraries were quantified in a Qubit 2.0 Fluorometer using the dsDNA HS Assay Kit (both Thermo Fisher). All libraries were pooled in equimolar ratios and sequenced on the NextSeq 500 sequencing system (Illumina)