title: GSM7113590: HIV112_V1; Homo sapiens; RNA-Seq
library_construction_protocol: To understand how the transcriptome of pDCs is impacted by HIV-1 infection and exogenous stimulation, we sorted pDCs from people with HIV-1 (PWH) on antiretroviral treatment before and after toll-like receptor 9 (TLR9) agonist treatment, as well as from healthy controls, and performed single-cell (sc)-RNA sequencing. Briefly after pDCs were isolated, cells and reagents were prepared and loaded into the chip and ran into the Chromium Controller for Gel Bead-In Emulsion (GEM) generation and barcoding. The input number of cells was estimated at 15-20,000 cells per sample. The Chromium Next GEM Single Cell 3' Gel beads v3.1 kit (10X Genomics, Pleasanton, CA, USA) was used to create GEMs following manufacturer's instruction. All GEMs generated were used for cDNA synthesis and library preparation using the Chromium Single Cell 3' Library Kit v3.1 (10X Genomics) following the manufacturer's instruction. scRNA-seq libraries were then prepared using GemCode Single Cell 3' Gel bead and library kit (10X Genomics) following the manufacturer's instruction. cDNA concentration of each sample was measured using a Tapestation 2200 system (Agilent).  Single-cell barcoded cDNA libraries were sequenced on an Illumina Illumina Novaseq6000 system (100-cycle cartridge) with a sequencing depth of at least 50,000 reads per cell.