title: GSM6925770: Embryo12; Sus scrofa; RNA-Seq
library_construction_protocol: Pig embryos and endometrial samples from days 9 and 12 of pregnancy were rinsed several times with DPBS, minced into small pieces with a scissor, and transferred to the pre-cooled EP tubes. For embryos, the minced tissues were digested in a pre-warmed enzyme cocktail Ⅰ, which contains papain, dispase and DNase Ⅰ, at 37°C for 30 min. While endometrial tissues were digested in an enzyme cocktail II, including type II collagenase, type IV collagenase, dispase and DNase Ⅰ. The dissociated cell suspension was terminated with DMEM medium containing 10% fetal bovine serum (FBS), passed through a 70-μm sterile cell strainer and centrifuged at 500g for 5 min at 4°C. After the supernatant was removed, the pelleted cells were suspended in red blood cell lysis buffer and incubated at room temperature for 5 min to exclude remaining red blood cells. Finally, the cell pellets were washed twice and resuspended in DPBS containing 0.04% bovine serum albumin (BSA) for further single-cell RNA sequencing analysis. Single-cell suspensions were loaded on a Chromium Single Cell Controller (10X Genomics) according to the manufacturer's instructions (Chromium Next GEM Single Cell 3' Reagent Kit V3 User Guide). All subsequent steps were performed following a standard manufacturer's protocols.