title: 640-3
design: The rice plants identified through screening with Sanger sequencing were transplanted into growth chambers and cultivated at 28C with a 12-hour light/12-hour dark cycle. After 5-6 weeks of cultivation, 10 cm leaf samples were collected from each plant, placed in selfsealing bags or 50 mL centrifuge tubes, rapidly frozen in liquid nitrogen, and stored in a -80C ultra-low temperature freezer. Two leaf samples were collected from each plant for backup. The DNA samples were sent to Molbreeding Company in Shijiazhuang, China for library construction, using the HuaDa DNBSEQ-T7 platform for resequencing.