title: GSM8526904: RNAseq, E13.5, sorted pre-granulosa cells, Tg(Enh8-mCherry) mouse line, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from ~40,000 sorted Sertoli or pre-granulosa cells of E11.5, E12.5, E13.5 and E15.5 gonads via FACS. RNA purification was performed using the RNeasy plus micro kit (Qiagen, 74034) according to the manufacturer instructions. cDNA libraries were prepared using the NEB Next Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB, E6420) according to the manufacturer's protocol. cDNAs were synthesized by reverse transcriptase enzyme, followed by cDNA amplification and cDNA clean-up using AMPure beads (Beckman coulter, Bc-a63881). cDNA was then fragmented, and adapters were ligated with unique indexes for each sample using the NEBNext® Multiplex Oligos for Illumina (NEB, E7335).