title: GSM8073624: GAT4.3NC; Mus musculus; OTHER
library_construction_protocol: The genomic DNA was extracted Lysis Buffer (50 mM Tris, 50 mM EDTA, 1% SDS, pH 8.0), Proteinase K and RNase A. DNA was precipitated by isopropanol. The sgRNA library readout was performed using a two-step PCR strategy, where the first PCR includes enough genomic DNA to preserve full library complexity and the second PCR adds appropriate sequencing adapters to the products from the first PCR.