title: GSM8037011: WT_ovaries_1_8st_rep2_Hi-C; Drosophila melanogaster; Hi-C
library_construction_protocol: For the in situ Hi-C experiments, ovaries were collected in PBS buffer (200 pairs per Hi-C), cross-linked with 1% of formaldehyde for 5 min and then incubated for 5 min with 125 mM Glycine. Then ovaries were washed with 1xPBS buffer for three times. The remaining in situ Hi-C protocol was performed as described previously (Rao et al (2014) Cell, 159:1665-1680). Hi-C libraries were prepared for sequencing using standard Illumina protocols