title: Mda5 RIP-Seq of mus musculus: adult female placenta
design: Placental tissues from E14.5 Adarf/f and AdarPKO mice were processed to extract input RNA using TRIzol Reagent. A second portion was lysed in dsRIP buffer (100 mM NaCl, 50 mM Tris-HCl pH 7.4, 3 mM MgCl2, 0.5% NP-40) with protease inhibitors and RNase inhibitor. Lysates were incubated with 5 mg Mda5 antibody (Proteintech, 66770-1-Ig) at 4C for 2 hours, followed by Protein G Dynabeads capture. RNA was extracted using TRIzol Reagent, rRNA-depleted, fragmented, and sequenced on the NovaSeq platform (Novogene, Beijing).