title: GSM7865910: Control; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow was isolated from tibias and femurs through flushing with complete DMEM.  Following erythrocyte lysis, lineage negative (Lin–) cells were enriched using magnetically-activated cell separation (MACS) columns following incubation with biotinylated lineage cocktail and anti-biotin magnetic beads.  Enriched Lin– cells were stained with biotinylated lineage cocktail, anti-cKit-PE, anti-biotin-PerCP-Vio700, annexin V-FITC, and DAPI.  Cells from each mouse were sorted as Lin– cKit+ Annexin V– DAPI–, and equal numbers of cells were pooled within each group for analysis by scRNAseq. single-cell 3' mRNA Transcriptomics; Single cell library was prepared using “Chromium Next GEM Chip G Single Cell Kit”, 16 rxns PN-1000127 and “Chromium Next GEM Single Cell 3ʹ Kit v3.1”, 4 rxns PN-1000269 and “Dual Index Kit TT Set A”, 96 rxns PN-1000215, following “Chromium Next GEM Single Cell 3' Reagent Kits v3.1(Dual Index) User Guide” (Document number CG000315).