title: ITS metabarcoding of grape must sample from winery L ferment 1A timepoint T1 year 2020
design: Paired-end sequence data (2x300bp) were quality trimmed (Trimmomatic v0.22), adaptor trimmed at the 3 end (cutadapt 1.2.1) and overlapped to form single synthetic reads (FLASH 1.2.11). These synthetic reads were then demuxed according to the inline barcode sequences, before trimming all adaptor, barcode and primer sequences from the 5' and 3' ends.