title: GSM7246248: shMETTL3_Input_rep2 [MeRIP]; Homo sapiens; OTHER
library_construction_protocol: Total RNA was isolated from chromatin-associated fraction of A375 cells. Non-ribosome RNA was further enriched from total RNA by using RiboMinus transcriptome isolation kit (Invitrogen). 1 μL 1:1000 diluted m6A spike-in from EpiMark N6-Methyladenosine Enrichment Kit (NEB #E1610S) was added to 1 μg non-ribosome RNA, followed by fragmentation according to previously published protocols. m6A-IP was performed using EpiMark N6-Methyladenosine Enrichment Kit following the manufacturer's protocols. Library preparation was performed by using SMARTer Stranded Total RNA-Seq Kit (Takara) v2 following manufacturer's protocols.