title: GSM7246185: A549 cells transiently transfected with scramble control siRNA, Vescicular RNA, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cellular and exosomal miRNAs extraction was executed, using respectively the miRNeasy Mini Kit (QIAGEN, Hilden, Germany), and the Total Exosome RNA & Protein Isolation Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. Library preparation was performed using  QIAseq miRNA Library Kit (Qiagen) following the manufacturer's instructions. RNA-Seq on a Illumina HiSeq NovaSeq 6000 instrument using a 75-bp, single-end sequencing configuration