title: GSM8526947: NK-92 shCtrl cells rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from NK-92-shCtrl/shmiR155HG cells stimulated with IL-2 (1000 U/mL) + IL-12 (10 ng/mL) for 12 h using AG RNAex Pro Reagent (Accurate Biotechnology), and the concentration and quantity of RNA was measured using a BioDrop μLite equipment (Biochrom Ltd). mRNA was isolated by Oligo(dT)-attached magnetic beads and then randomly fragmented in fragmentation buffer. First-strand cDNA was synthesized with fragmented mRNA as template and random hexamers as primers. Adapters were added by incubating to end repair. After RNA-seq libraries were constructed, qualified libraries were uploaded on Illumina NovaSeq 6000 platform for sequencing with read length of PE150.