title: GSM8017769: SRE, Cut&Run, Bmi1, Rep1; Homo sapiens; OTHER
library_construction_protocol: CUT&RUN was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher) on whole cells. Libraries were generated using the NEBNext Ultra II DNA Library Prep kit (NEB). For transcription factors (BMI-1 and RING1B), size selection was optimized for small fragments (end prep for 20C for 30 minutes followed by 50C for 60 minutes, ligation AMPure XP cleanup at 1.75X, annealing temperature of 65C for library amplification, and library AMPure XP cleanup using 1st round negative selection at 0.8X followed by a 2nd round positive selection at 1.2X). Libraries were sequenced with illumina HiSeq, paired end 150 bp length reads.