title: Single Cell Multiome ATAC
design: Unsorted bone marrow mononuclear cells were analyzed by single-nuclei RNA sequencing coupled with single-nuclei Assay for Transposase-Accessible Chromatin sequencing. Dead cells and granulocytes were removed using Miltenyi dead cell removal beads (130-090-101) and CD66abce microbeads (granulocyte depletion kit 130-092-393). Nuclei were isolated according to the 10X Genomics protocol (CG000365 Rev C). Library preparation was done using the 10X Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit (PN-1000283) according to the manufacturer's instructions. Ribosomal, mitochondrial, and non-transcriptomic fragments were depleted using Jumpcode Genomics CRISPRclean Single Cell RNA Boost Kit (Human-KIT1018).