title: ITS2 amplicon of gut metagenome
design: The amplicon sequencing process was carried out by amplifying the ITS2 region using forward primer ITS3 (GCATCGATGAAGAACGCAGC) and reverse primer ITS4 (TCCTCCGCTTATTGATATGC). The sequencing libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina.