title: Study of the cytoplasmic male sterility induced by the Shahdara cytoplasm in Arabidopsis thaliana
design: Study of the cytoplasmic male sterility induced by the Shahdara cytoplasm in Arabidopsis thaliana
library_construction_protocol: T3BC1 seeds = selfing seeds from T2BC1 plants issued from mTalen mutagenesis of [Sha]CviL3S plants. BC made at the T1 generation with Cvi-0, T1BC1 selected from the absence of the mTalen construct and T2BC1 plants selected for homozygous Cvi-0 allele at locus L3. T3BC1 seeds sterilized and sown in vitro on standard Arabidopsis medium in room chamber after 3 days stratification in the dark. For each line, whole genomic DNA extracted from ~ 1.5 g of frozen material using the NucleoSpinPlantII Maxi kit from Macherey Nagel according to supplier instructions. Standard genomic library