title: GSM7247324: MCF7-NUTF2 biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy kit (QIAGEN). mRNA was selectively isolated from total RNA (500ng input) using poly-T oligos attached to magnetic beads according to the manufacturer's guidelines in the NEBNext poly(A) mRNA magnetic isolation module (Cat. #: E7490, NEB, Ipswich, MA). The library preparation from the enriched mRNA was conducted according to NEBNext Ultra II Directional RNA kit