title: GSM8166958: Testis_3Month_H3K27ac_Cuttag; Mus musculus; OTHER
library_construction_protocol: CUT&Tag for profiling histone H3K27ac was performed using Hyperactive Universal CUT&Tag Assay Kit for Illumina (vazyme, TD903-02) according to the instructions, and anti-H3K27ac antibody  (abcam, ab4729) were used to enrich target DNA fragments of Cd34-mesenchymal cells obtained by flow sorting. TruePrep Index Kit for Illumina (v21.2, vazyme, TD202) were then used to DNA library construction according to the instructions.