title: 16S data of marula wine
design: The amplicons were diluted with 10 mM Tris pH 8.5 to 4 nM, and 5 L from each library were mixed for pooling libraries with unique indices. All the libraries were pooled for one MiSeq run. Pooled PCR amplicons were purified and indexed using the Nextera XT primers (Illumina Inc., CA, USA). Thereafter, the amplicon library was quantified, normalised and pooled prior to loading on the MiSeq flow cell for a 2300 paired-end sequencing. Sequence reads were then de-multiplexed using the on-system MiSeq reporter software.