title: GSM7247503: T21 RNA-seq repA; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from the cells via Trizol extraction. NEBNext rRNA Depletion kit was used to remove rRNA. NEBNext Ultra II RNA was used to transform the  RNA into an RNA-seq library. The product was amplified 15 plus or minus 3 cycles and products >150 bp (insert > 70 bp) were size selected with 1X AMPure XP beads (Beckman) before being sequenced.