title: GSM8526939: WT CD8 T cells, LCMV Arm, restimulated; Mus musculus; RNA-Seq
library_construction_protocol: Mouse P14 cells were flow sorted from the spleen of Vbeta5 hosts (CD45.1) transferred with WT or PD-1 KO Tcf7GFP P14 cells at d28 post LCMV infection. WT P14 cells were sorted from hosts infected with either Armstrong (Arm) or clone13 (cl13), while PD-1 KO Tcf7GFP P14 cells were exposed to cl13 only.  The sorted cells were restimulated in vitro with plate bound anti-CD3/28 antibodies for 4 h (stimulated) or were cultured without activation (rested) and then subjected to scRNAseq analysis. Rested and stimulated cells derived from the same donor mouse. 10x Genomics Dual Single Cell 3' v3 chemistry