title: GSM6661749: C57BL/6 mice with BCP_4; Mus musculus; miRNA-Seq
library_construction_protocol: Samples of spinal cord were homogenized in 1 mL TRIzol reagent (Invitrogen, USA) for 5 min at room temperature, followed by chloroform (Sigma-Aldric, USA) extraction for 2 min and isopropanol (Sigma-Aldric, USA) precipitation for 10 min. The purity of total RNA was determined using Aglient 2100 Bioanalyzer (Aglient, USA). Total RNA was fragmented and reversely transcribed into cDNA using TIANSeq RNA Frag/cDNA Synthesis Module (TIANGEN, Beijing, China) after rRNA was removed by TIANSeq rRNA Depletion Kit (TIANGEN, Beijing, China). cDNA library construction was performed by TIANSeq Fast DNA Library (illumina) Kit (TIANGEN, Beijing, China) after the purification and enrichment of cDNA fragments using TIANSeq Size Selection DNA Beads (TIANGEN, Beijing, China). RNA-sequencing was processed using a high-throughput sequencing platform (illumine, USA) to obtain sequencing reads of each sample.