title: GSM8214055: Mouse liver RNAseq data_WT and KO Chow 21 days - 1 WT; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from the liver lobes of wild-type (WT) and Mtm1 knockout (KO) mice at postnatal day 21 (PND 21) and postnatal day 35 (PND 35). The extraction followed the Qiagen RNeasy kit protocol and was quantified using a NanoDropTM2000 Spectrophotometer. The RNA quality was assessed using the Agilent 2100 BioAnalyzer and RNA 6000 Nano Kit at The Centre for Applied Genomics (TCAG). Whole-transcriptome analysis was conducted through RNA sequencing at TCAG using the NEB Ultra II Directional poly(A) mRNA library prep kit, followed by paired-end sequencing on a NovaSeq 6000 system.