title: mtDNA-Seq of U2OS cells
design: DNA was extracted from each flash frozen cell pellet. The entire mtDNA was amplified with long-range PCR and the amplicons purified, tagged and indexed applying Illumina Nextera XT reagents and sequenced at high depth (>1,000x) with Illumina Novaseq chemistry. Each DNA sample was sequenced twice, including independent PCR amplification, library preparation and sequencing.