title: GSM8262578: male, LKO, rep1; Mus musculus; RNA-Seq
library_construction_protocol: About 50 to 100 mg of liver tissues from two mice were used for total RNA extraction using TRIzol Reagent. A total RNA sample from two livers tissues was used to contruct one library. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library. This was done by Novogene. Stranded bulk RNA-seq