title: GSM8035924: APOE 3/3 iMG LD low PE 2 [RNA-seq]; Homo sapiens; RNA-Seq
library_construction_protocol: iMicroglia RNA was isolated from the cell pellets using an RNeasy Plus Micro kit (Qiagen, 74034). mRNA was transcribed into full-length complementary DNA using a SMART-Seq v4 Ultra Low Input RNA kit (Clontech) according to the manufacturer's instructions. Full-length cDNA (150 pg) was processed using a Nextera XT DNA library preparation kit (Illumina) according to the manufacturer's protocol. Library quality was verified using the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA kit. Sequencing was carried out using an Illumina NexSeq 550, paired-end, 2× 100 bp depth sequencer.