title: GSM6585648: anti-CD40L treated LNSC scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: C57BL/6 mice received one dose of 250 ug anti-CD40L i.v. for one day. After enzymatic digestion of the lymph nodes (LNs)from  C57BL/6 mice received one dose of 250 ug anti-CD40L i.v. for one day, CD45- cells were purified with the CD45 negative selection beads and were stained with anti-mouse CD45 before sorting for viable CD45- cells. C57BL/6 mice received one dose of 250 ug anti-CD40L i.v. for one day lymph node stromal cells were isolated for scRNA-seq, more than 2x104 cells were run on the 10X Chromium Controller (10X Genomics) to partition single cells with uniquely barcoded beads and processed for sequencing library preparation using the Chromium Single Cell 3' Reagent Kit. cDNA libraries were sequenced on a NovaSeq 6000 sequencing system.