title: GSM8017467: Ctrl, 14 DPI; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow cells were obtained through flushing the femurs and stained with Lin and Kit as described above. Lin-Kit+, Lin-Kit- and Lin+ populations from three individual mice were then sorted and mixed with a ratio of 5:4:1 for 10x Genomics single-cell sample preparation. The single cells were centrifuged and resuspended before finally load to 10x chromium controller for Gel Beads-in-Emulsion (GEMs) generation. cDNA library construction was performed following the standard protocol offered by 10x Genomics (single-cell, 3' V2).