title: GSM8494374: SVI cells, unstretched, experiment 2, 3; Mus musculus; RNA-Seq
library_construction_protocol: Cells were processed in Tri-Reagent (Sigma-Aldrich) according to manufacturer's instructions, followed by DNA-digestion steps  using the RNAase-Free DNase I Kit and RNA Clean-Up and Concentration kit according to manufacturer's instructions (both Norgen Biotek Corp., Thorold, ON, Canada). Briefly, after quality assessment using Tape Station 4200 (Agilent, Santa Clara, CA, USA), a PolyA library was prepared based on 500ng total RNA per sample using the TruSeq stranded mRNA kit (Illumina, San Diego, CA, USA).  Subsequently, for sequencing 200pM of the final library were loaded on the NovaSeq 6000 System, S1 FlowCell (2 x 100 bp pair-end reads). A sequencing depth of ~ 200 Mio Cluster per sample was obtained, recorded at the Competence Centre for Genomic analysis in Kiel.