title: GSM8164431: 15_Young_MPP; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq was performed as previously described (PMID 33710340). Briefly, sorted cells were used to synthesize the first strand of cDNA with PrimeScript RT reagent kit (TAKARA Bio Inc.) and not so random primers. The first strand was treated with Klenow Fragments (3'-5' exo-; New England Biolabs Inc.) and complement chains of not so random primers to synthesize the second-strand cDNA. After purifying double-strand cDNA, the library for RNA-seq was prepared and amplified using Nextera XT DNA sample prep kit (Illumina Inc.).