title: Nanopore sequencing of Plantibacter sp. RU18
design: The RU18 isolate was cultured at 18C with 160 rpm of agitation in LB medium. DNA was extracted from the cultures with the commercial kit DNeasy Blood & Tissue (QIAGEN, Hilden, Germany) following the manufacturer's instructions, and quantified by fluorimetric method with the Qubit Broad-Range (BR) Kit (Invitrogen, Waltham, MA, USA). DNA library construction was performed with the commercial kit SQK-LSK110 following the manufacturer's instructions and sequencing was done with the FLO-MIN106D Flow Cell R9 Version on a MinION sequencer (Oxford Nanopore Technologies, Oxford, UK).The basecalling was performed with dorado v0.5.2 (Oxford Nanopore Technologies, Oxford, UK) with the dna_r9.4.1_e8_sup@v3.6 model.