title: RNAseq of Homo Sapiens: normal esophagus
design: RNA sequencing was performed according to the previous protocol used to generate ANTE collection of healthy tissue RNAseq profiles (Suntsova et al (2019)).  To isolate RNA preps, 10 M-thick paraffin slices were trimmed from each FFPE tissue block with a microtome. RNA was extracted from FFPE slices using QIAGEN RNeasy FFPE Kit following the manufacturers protocol. RNA 6000 Nano or Qubit RNA Assay kits were used to measure RNA concentration. RNA Integrity Number (RIN) was measured using Agilent 2100 bio-Analyzer. For depletion of ribosomal RNA and library construction, KAPA RNA Hyper with rRNA erase kit (HMR only) was used. Different adaptors were used for multiplexing samples in a single sequencing run. Library concentrations were measured using the Qubit ds DNA HS Assay kit (Life Technologies) and Agilent Tapestation (Agilent). RNA sequencing was performed using Illumina NextSeq550 equipment for single-end sequencing, 70 bp read length).