title: GSM8017940: wild type  Mre11-HA6 t5 HA-ChIP; Saccharomyces cerevisiae; Nakaseomyces glabratus; ChIP-Seq
library_construction_protocol: Cells were opened by FastPrep-24™ 5G Instrument (MP Biomedicals) followed by sonication with Bioruptor® Pico instrument. After removing the cell debris, the supernatant was used for the ChIP with anti-HA antibody. To reverse crosslink, all the samples were incubated in 65°C overnight. DNA purification was conducted by regular phenol/chloroform/isoamyl alcohol extraction. Library preparation was performed using NEBNext Ultra II DNA Library Prep Kit according to the manufacturer´s protocol. Amplified library DNA was purified and subjected to 300 bp - 500 bp size selection using Agencourt AMPure XP beads (Beckman Coulter) and quantified by NanoDrop 3300 Fluorospectrometer and Agilent 2100 Bioanalyzer.