title: GSM8163969: TSKb20_TCR-seq; Mus musculus; OTHER
library_construction_protocol: T cells were isolated from skeletal muscle using an EDTA/Collagenase digest followed by a Percoll gradient to enrich for lymphocytes. Cells were stained for viability, CD8, and TSKb20 tetramer then sorted on a cell sorter. TSKb20-tetramer bound and Nur77-GFP+ T cell populations were each sorted out from 5 individual mice then pooled together. Libraries for scRNA-seq were prepared according to the manufacturer's instructions, using Chromium Single Cell 5' Dual Index Library & Gel Bead Kit, Chromium Single Cell A Chip Kit, and Chromium Multiplex Kit (10x Genomics). Amplified cDNA was used to generate single-cell TCRαβ libraries using Chromium Single Cell V(D)J Enrichment kit for mouse T cells (10x Genomics).