title: GSM7795591: Quadriceps, input; Homo sapiens; ChIP-Seq
library_construction_protocol: Samples from the healthy limb and pre-surgery time point in the injured limb were not assessed due to insufficient chromatin to complete analysis consequent to low VDR expression in these samples. Briefly, crosslinked chromatin was incubated with 30 μL of VDR antibodies (sc-1008, Santa Cruz Biotechnology, Inc. Dallas, Texas) and immunoprecipitated. Purified DNA (20 μg) was prepared for sequencing using the ChIP-IT® ChIP-seq kit (Active Motif, Carlsbad, CA). Libraries were sequenced on an Illumina NextSeq 500 (75-nt single-end). Reads were mapped to the GRCh38 genome using BWA with default settings