title: 16S rRNA amplicon sequencing of host fitness 2
design: PCR amplicon libraries using uniquely barcoded primers flanking the V1-V2 hypervariable regions  with fused MiSeq adapters and heterogeneity spacers were generated. Amplicons were size checked and purified ,quantified using Quant-iT PicoGreen kit (Invitrogen) and equal amounts of the purified PCR products were pooled for subsequent Illumina sequencing. Amplicon sequencing was performed on the Illumina MiSeq platform with v3 chemistry (2300cycle kit) according to the manufacturer's instructions .