title: GSM8255403: SCB_073; Homo sapiens; RNA-Seq
library_construction_protocol: S6w1 SC-islets were dispersed by incubating with 2 ml of Accutase solution containing RockI (2 µL/mL) for 10 minutes at 37°C. Isolated single cells were strained with 40micron cell strainer and then counted for live dead staining to measure live cells. A cell suspension of 1 million cells per mL was resuspended for total RNA isolation and libraray preperation. Samples of S6w1 SC-islet Cells were prepared following the protocol of the 10X Genomics Single Cell 3' V3.1 Reagent Kit. They were processed using the 10X Genomic Chromium Controller for partitioning and barcoding, and the cDNA library was generated at the Advanced Cell Exploration Core- University of Alberta. The total cell concentration was assessed using the Countess, followed by sequencing on the NovaSeq 6000 System (Illumina) at the core facility at Centre for Health Genomics and Informatics, University of Calgary. The resulting FASTQ files were aligned using Cell Ranger V.7.0.0 with Single Cell 3' V3 chemistry on the 10X Cloud pipeline.