title: GSM8036358: human-induced pluripotent stem cells (hiPSCs)-derived hepatocytes,IL-1β 20ng/ml, bio rep 2 [S_18]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher) according to the manufacturer's instruction. RNA integrity and quantitation were determined using Agilent 2100 bioanalyzer. Oligo(dT) purified RNAs were used to construct stranded libraries, followed by quality control using Agilent 2100 bioanalyzer. Approximately 200ng of total RNA was used as input. Libraries were prepared according to Illumina's instructions for the TruSeq long Stranded mRNA LT Sample Prep Kit (Catalog IDs: RS-122-2101, RS-122-2102). Paired-end Illumina sequencing was performed on the Illumina NovaSeq 6000 system to generate approximately 20 million reads per sample.