title: GSM6857381: 22Rv1-WT-BRD4-ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: RNA-seq:Total RNA was extracted using TRIzol according to the manufacturer's instruction. Then, ribosomal RNA was deleted from 5 μg of total extracted RNA using the Ribo-Zero Gold Kit, using Illumina novaseq 6000. ChIRP-seq: Cells were fixed with glutaraldehyde  for 10 min and nucleus was isolated and sheared to 100-500 bp fragments by sonication, then hybridized with DNA probes. Libraries were prepared using the NEBNext DNA library kit according to the manufacturer's instructions (New England BioLabs), using Illumina novaseq 6000. ChIP-sq:Cells were fixed with formaldehyde  for 10 min and nucleus was isolated and sheared to 200-500 bp DNA fragments by sonication, then immunoprecipitated with the corresponding antibodies. Libraries were prepared using the NEBNext DNA library kit according to the manufacturer's instructions (New England BioLabs), using Illumina novaseq 6000. Cut and tag: The experiments were performed using Hyperactive Universal  CUT & Tag Assay Kit (Vazyme, TD901). Briefly, Cells were incubated with Concanavalin A- Coated beads and then incubated with antibody. DNA sequencing library was built by pA-Tn5 transpose, then sequenced by Illumina novaseq 6000. RNA-seq, ChIRP-seq , ChIP-seq and Cut and tag  libraries were prepared for sequencing using standard Illumina protocols