title: Prokaryotes Community Analysis of Rhizosphere of rice plants treated with MiZax3, tillering stage
design: Prokaryotes community assembly was analyzed by using the V4 hyper-variable region of the 16S rDNA using 515F-805R primers pair (Parada et al. 2016; Apprill et al. 2015) linked with the Illumina adapters overhang. Amplification on organellar rRNA was prevented by peptide nucleic acids (PNAs) clamping using universal pPNA and mPNA clamps for plastidial and mitochondrial sequences following the manufacturers protocol (PNA Bio Inc, Newbury Park, CA).