title: GSM8296686: LD_gut_3; Dendrobaena veneta; RNA-Seq
library_construction_protocol: Total RNA was extracted from gut and cerebral ganglion tissues, using first Trizol reagent then followed by the Quick-RNA™ MiniPrep kit (Zymo Research, Irvine, USA) according to manufacturer's protocol Libraries were prepared according to Illumina's instructions. Briefly, mRNAs were purified using Poly(A) selection from total RNA and then fragmented. First strand cDNA was synthesized using random priming, followed by the synthesis of the second strand. The resulting double-strand cDNA was end repaired, phosphorylated and A-tailed. Adapter ligation and PCR amplification were performed, rendering the library ready for Illumina flow cell clustering and sequencing on an Illumina HiSeq  X Ten following the manufacturer's protocols.