title: Mouse OC (KO_MGCs-3)
design: For osteoclast differentiation analysis, BMMs were incubated in osteoclastogenic medium containing 30 ng/mL M-CSF and 50 ng/mL RANKL (Peprotech) for 46 days. After osteoclast differentiation, SNCs were collected using cell dissociation buffer treatment, and MNCs were directly lysed after thorough washing with PBS.