title: GSM7350514: IRAK1 reference allele G single cell clone 9; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from HEK293T cells using TRIzol (Thermo Fisher Scientific, Cat# 15596026). Each 500 ﻿μl TRIzol-lysed solution was mixed with 100 ﻿μl chloroform (Fisher Chemical, Cat# C298-500) to allow phase separation. The upper aqueous phase was transferred and mixed with equal volume ethanol (200 proof, Fisher BioReagents). The mixture was loaded to the column supplied by the Direct-zol RNA Miniprep Plus kit (Zymo Research, Cat# R2072) to isolate total RNA following the manufacturer's protocol. Total RNA was used to generate RNA-Seq libraries using standard protocols at TCGB UCLA.