title: GSM8296284: CTRL_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using the RNeasy mini kit (QIAGEN), according to the manufacturer's instructions. 200ng of RNA from each sample was used to prepare sequencing libraries. Libraries were prepared with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB # E7490S) and NEBNext Ultra II Directional RNA Library Prep Kit (NEB # E7760L), per manufacturer's instructions. Library cDNA were equally pooled at 10 nM for sequencing.