title: Long-read 16S: female urine microbiome
design: DNA was extracted from each urine sample using the Norgen Urine DNA Isolation kit. DNA was normalized to 500 pg/microliter in elution buffer. The DNA was amplified using the following primers: AGRGTTYGATYMTGGCTCAG (27F) and RGYTACCTTGTTACGACTT (1492R). A SMRTbell library was constructed from the PCR products.