title: GSM6925795: atdgcr14l-1, 200 mM NaCl, 0h, Rep 3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNAs were extracted using Spectrum Plant Total RNA kit (Sigma) following manufacturer's protocols. mRNAs with poly A tail were enriched from total RNAs using oligo dT beads. mRNA molecules were fragmented into samll pieces. The fragmented mRNA was synthesized into first strand cDNA using random primers. The second strand cDNA was synthesized with dUTP instead of dTTP. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments. Digest the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG) and perform PCR amplification. After quality control and circularization, libraries were amplified to make DNA nanoball for sequencing on DNBSEQ platform.