title: RNA-seq of roots of Schrenkiella parvula seedlings treated with 0mM or 175mM NaCl for 0 h, 3 h, 24 h and 48 h.
design: RNA-seq of roots of Schrenkiella parvula seedlings treated with 0mM or 175mM NaCl for 0 h, 3 h, 24 h and 48 h.
library_construction_protocol: Root materials were harvested by razor blades from S. parvula seedlings grown for 4 days on ½ MS agar plates and transferred on mesh to new medium supplemented with 0 mM or 175 mM NaCl for 0 h, 3 h, 24 h or 48 h. Seeds of Schrenkiella parvula (Lake Tuz ecotype) were stratified in dark at 4°C for 7 days and were surface-sterilized in 30% (v/v) sodium hypochlorite and washed 4 times with H2O. Seeds were geminated on nylon mesh strips on ½-strength Murashige-Skoog (½ MS) medium including vitamins (Duchefa), supplemented with 0.1% 2-(N-morpholino) ethanesulfonic acid (MES, Duchefa), 1% Daishin agar (Duchefa) and pH was adjusted with KOH to 5.75. Plates were placed at 70° racks in the growth chamber with 16 h light:8 h dark, 130 μmol m-2 s-1 LED light, 21°C and 70% relative humidity. Samples were frozen in liquid nitrogen and ground before RNA extraction. Total RNA was isolated using NZY Total RNA Isolation kit (NZYtech). RNA library preparation was conducted by Novogene Co., LTD (United Kingdom)