title: GSM8036506: WT_2; Mus musculus; RNA-Seq
library_construction_protocol: Large intestine from Stingfl/fl and Stingfl/fl RORγtcre mice were dissected and fat tissues were removed. Intestines were opened longitudinally, extensively cleaned with cold Dulbecco's PBS (Corning) and cut approximately into 0.5-cm sections. Cut tissues were incubated in Hanks' balanced salt solution) containing 5 mM EDTA (Thermo Fisher Scientific), 1 mM dithiothreitol (Sigma-Aldrich) and 2% fetal bovine serum twice at 37 °C for 20 min to dissociate epithelial cells. Tissues were vortexed, transferred to digestion buffer containing collagenase III (1 mg mL-1; Worthington), dispase (0.4 U mL-1; Thermo Fisher Scientific), DNase I (20 μg mL-1 ;Sigma-Aldrich) and 10% FBS in RPMI 1640 (Corning), and incubated in a shaker at 200 rpm for 1 hour at 37°C. Leukocytes were enriched by 40/80% Percoll gradient (GE Healthcare). Library construction was performed by epigenomics core at Weill Cornell Medicine.