title: GSM6970624: MycKO 5 month, omental adipose tissue [K5F5116]; Mus musculus; RNA-Seq
library_construction_protocol: RNAs were isolated from mouse tissues using RNeasy kit,according to the directions of the supplier (Qiagen, Inc. Germantown, MD). Exceptions to this were made in the case of adipose tissue and skeletal muscle for which we utilized an RNeasy Lipid Tissue extraction Kit(Qiagen, Inc., Germantown, MD). Sequencing libraries were generated using a NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs, Beverly, MA).Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms.