title: GSM6662012: ML infected liver, 30M, rep1; Dasypus novemcinctus; RNA-Seq
library_construction_protocol: After storage in RNAlater, total RNA was extracted using TRIzol and chloroform/isopropanol. Total RNA was resuspended in distilled, Rnase-free water and quantified using a Nanodrop ND-1000 Spectrophotometer. 1-2 μg total RNA was used to prepare the sequencing libraries. Library preparation involved oligo (dT) magnetic bead mRNA enrichment, highly strand-specific dUTP method using KAPA Stranded RNA-Seq Library Prep Kit, library size distribution and yield QC with Agilent 2100 Bioanalyzer and by absolute quantification qPCR.