title: GSM6661490: d8; Macaca fascicularis; OTHER
library_construction_protocol: Genomic DNAs were purified from 10,000 - 20,000 cells by using Nucleospin Tissue XS (Macherey-Nagel, 740901) and eluted in 10µL Elution buffer. DNA concentration was quantitated by using Qubit dsDNA HS assay kit (ThermoFisher Scientific) and 20-140 ng of gDNA were mixed with 1pg of pUP19 DNA and 20pg of unmethylated lambda DNA in 50µL total volume. Genomic DNAs were fragmented by sonication using Covaris focused-sonicator E220 (peak incident power 140W, Duty factor 10%, Cycles per burst 200 and Treatment time 130 msec). The fragmented DNA samples were purified by two-rounds of Axygen beads purification (0.7×, then 0.9× volumes). Then, all fragments were applied to library construction using NEBNext Enzymatic Methy-seq Kit (New England Biolabs, E7120) according to manufacturer's instructions. Library concentration and fragment size were evaluated with KAPA Library quantification kits (Nippon Genetics, KK4828) and Labchip GX Touch HT (Perkin Elmer). Enzymatic Methyl-seq (EMseq)