title: GSM8372560: Marmoset, Control, 1; Callithrix jacchus; RNA-Seq
library_construction_protocol: Brain tissues were homogenized in a homogenizer with TRIzol reagent and incubated at room temperature for 5 minutes. Chloroform equivalent to 20% of Trizol solution was added to the homogenate, mixed, and left at room temperature for 3 minutes, then centrifuged at 12,000 x g for 10 minutes at 4°C to remove insoluble material (extracellular membrane, polysaccharides, and polymeric DNA). The supernatant was transferred to a new tube, mixed with an equal volume of 2-propanol, and centrifuged at 12,000 × g for 15 min at 4°C. The precipitate was rinsed with 70% ethanol and dissolved in TE buffer. The resulting total RNA was subjected to DNA degradation using DNase I. Sequencing libraries from bulk tissues were prepared with NEBNext™ Poly(A) mRNA Magnetic Isolation Module and NEBNext™ UltraTM II Directional RNA Library Prep Kit.