title: GSM8213760: M2 conditioned medium 1; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted from LNCaP-C42B cells by following instructions from RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Library was constructed using NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, New England BioLabs, Ipswich, MA) and NEBNext Ultra II Directional RNA Library Prep Kit (E7765, New England BioLabs, Ipswich, MA) according to manufacturer's protocol.