title: GSM7256810: E17.5_RNA_wildtype_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Tissue from E11.5 or E17.5 diencephalon was snap-frozen, then thawed and homogenized. Each replicate consists of tissue from 3 (at E17.5) or 6 (at E11.5) pooled embryos from the same litter. RNA was collected using the Qiagen miRNeasy Kit (#74106). Libraries were prepared using polyA selection (Roche Kapa mRNA  Hyper Prep Cat# KR1352) and sequenced (2×150 bp) using standard Illumina protocols on an Illumina NovaSeq 6000.