title: GSM8164749: Plasma, Diabetic kidney disease, rep8; Homo sapiens; miRNA-Seq
library_construction_protocol: Whole blood was collected using a vacuette needle into an ethylenediaminetetraacetic acid (EDTA) collection tube for plasma isolation. Following centrifugation at 3,000 g for 10 minutes at 4°C, the supernatant was pipetted into 3 x 1 ml aliquots in RNase/DNase-free tubes and frozen at -80°C. RNA was extracted from 600 μl of plasma using the NextPrep™ MagnaZol™ cfRNA Isolation Kit (Bioo Scientific Corp, Texas, USA), following the manufacturer's instructions, with purified RNA eluted in 16μl of nuclease-free water. RNA was quantified using the Qubit miRNA Assay kit (ThermoFisher Scientific, San Jose, USA). miRNA libraries were prepared using QIAseq miRNA Library Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. Library concentrations were quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and quality control of library was performed using a High Sensitivity NGS Fragment Analysis Kit (Agilent Technologies, Santa Clara, CA, USA).