title: GSM6721484: 50 nM PTX 3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested for RNA extraction following the RNeasy Mini Kit protocol (Cat No. 74104; Qiagen). RNA was eluted with 40 µL of RNase-free water. RNA concentration was measured with a spectrophotometer (BioDrop). Libraries were prepared from isolated mRNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA) and NEBNext Ultra II Directional RNA Library Preparation Kit (New England Biolabs, USA).