title: Second round of hub screening in K562 Replicate 1 Day 20
design: 1. pgRNA-expressing lentivirus was used to infect K562-cas9 cells. 2. The cells were sorted and maintained for an extended period before being harvested on various days. 3. Genomic DNA was extracted, and genotyping was conducted on the barcoding sgRNA region. 4. PCR amplification was performed to add Illumina Nextera adapters.