title: GSM8527024: Subject9_trametinib; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Rneasy Plus minikit (Qiagen) Ribosomal RNA was removed using the RiboErase kit (Kapa Biosystems). Remaining RNA was fragmented and reverse-transcribed using SuperScript III RT enzyme (Life Technologies). Following second strand synthesis, dual indexes were ligated and the library amplified prior to sequencing on an Illumina NovaSeq X Plus to generate paired-end 150bp reads.