title: GSM7865941: ALCAM siRNA TGFbeta rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (NEB, cat no T2010) following the manufacturer's instructions. RNA-Seq libraries were generated using the QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) following the manufacturer's instructions. Libraries were indexed with the i5 6 nt Dual Indexing Add-on Kit (Lexogen) to obtain unique dual indexing. For each sample, 80-200 ng of total RNA was used for library preparation.