title: GSM7247343: ko cardiac macrophage, mi-d5, biol rep3; Mus musculus; RNA-Seq
library_construction_protocol: Mice were sacrificed by CO2 anesthetization. The heart was rapidly excised, cut into little blocks and soaked in digested buffer to get single cell suspension. The cardiac macrophages were enriched using UltraPure Anti-F4/80 Microbeads (130-110-443, Miltenyi Biotec). Total RNA was extracted using MJzol animal RNA Extraction Kit(MagBeads) (Cat#T102096)following the manufacturer's instructions and checked for a RIN number to inspect RNA integrity by an Agilent 4200 TapeStation (Agilent technologies, Santa Clara, CA, US). Library construction was perfromed following the steps of mRNA isolation, fragmentation, first strand cDNA synthesis, second strand cDNA synthesis, end repair, adding A at the 3 'end, connecting connectors, enrichment, using Dual-mode mRNA Library Prep Kit(Borroen), Agilent High Sensitivity DNA Kit(Agilent) and Agilent D1000 Reagents(Agilent).