title: GSM7350353: Ko-1 Heart, E14.5; Mus musculus; RNA-Seq
library_construction_protocol: Embryonic hearts of E14.5 were collected respectively and total RNA were isolated using TRIzol Reagent (Invitrogen, Carlsbad, USA). NanoDrop 2000 spectrophotometer was applied to evaluate the purity and quantity of RNA. RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.