title: GSM7744875: svLEC_tgfb1_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from quadruplicate samples of svLEC cells that were either left untreated or treated for 72 h with 10 ng/ml TGFB1 (R&D Systems, Abingdon, UK), using RNeasy Mini Kit (Qiagen, Valencia, CA) according to the manufacturer´s protocol. Libraries were prepared using TruSeq Stranded mRNA kit (Illumina) and sequenced on the Illumina Nextseq 550 platform, at single end mode and generating 75 bp reads.