title: GSM7831813: Pa-17F,grade 1,  MDD 1, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: scRNA-seq: cut the outer layer of AF , then the inner layer of AF), and then  start to achieve NP. After that we washed the NP tissues with 0.9% normal saline three times. And transport it in ice-cold preservation solution. bulk RNA-seq: Total RNA was extracted from NP tissue, and treated with the GeneRead™ rRNA Depletion kit (Qiagen) to remove ribosomal RNA scRNA-seq: Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Briefly, scRNA-seq data analysis was performed by NovelBio Bio-Pharm Technology Co.,Ltd. with NovelBrain Cloud Analysis Platform. We applied fastp with default parameter filtering of the adaptor sequence to remove the low-quality reads and obtain the clean data. Then the feature-barcode matrices were obtained by aligning reads to the human genome (GRCh38 Ensemble: version 104) using CellRanger v6.1.1. We performed the down sample analysis of samples sequenced according to the mapped barcoded reads per cell of each sample and finally created the aggregated matrix. Cells containing over 200 expressed genes and a mitochondrial UMI rate below 20% passed the cell quality filtering and mitochondrial genes were removed from the expression table. bulk RNA-seq: a VAHTS Stranded RNA-seq Library preparation kit for Illumina (Vazyme) was used to construct strand-specific libraries