title: GSM6721623: old_p21_1 [f7_7_P21_609_S7]; Mus musculus; RNA-Seq
library_construction_protocol: Lungs were homogenised using pellet pestles and RNA extraction was performed using the Isolate II RNA mini kit according to manufacturer's instruction An input of 100 ng of total RNA was prepared and indexed for illumina sequencing using the TruSeq RNA sample Prep Kit with RiboGlobin depletion as per manufacturer's instruction. The indexed libraries were pooled and diluted to 1.5 pM for paired end sequencing (2 x 81 cycles) on a NextSeq 500 instrument using the v2 150 cycle High Output kit as per manufacturer's instructions.