title: RNA-Seq of mus musculus: adult female spinal cord
design: Samples containing total RNA were processed with an Illumina TruSeq Stranded mRNA Library Prep Kit (Illumina Inc., San Diego, CA, USA) to create cDNA libraries. Following bead-based purification of the poly-A mRNA this fraction was used for RNA fragmentation, first- and second-strand cDNA synthesis, end repair, adapter ligation, PCR amplification and subsequent PCR product purification. All library preparation steps were performed in accordance with the manufacturers instructions.