title: GSM8526733: DMD2_(Beckman)_scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: Organoid 12 weeks post differentiation were dissociated into single cells upon incubation for 1hr with solution containing papain and EDTA. Upon dissociation, we estimated the cell number and viability; then cells were resuspended on solution containing 0.5% BSA in PBS to reach concentration of 390 cells per ul. The cDNA library was prepared using the Chromium Single Cell 3′ Reagent Kits (v3): Single Cell 3′ Library & Gel Bead Kit v3 (PN-1000075), Single Cell B Chip Kit (PN-1000073) and i7 Multiplex Kit (PN-120262) (10x Genomics).