title: RNA Sequencing for Brangus_HR_B20_postT12: peripheral blood leukocytes
design: Leukocytes where isolated from whole blood and preserved frozen in Qiazol reagent (QIAGEN, USA) until extraction. Total RNA isolation was carried out with the miRNeasy mini kit (QIAGEN, USA). The cDNA libraries were prepared with the TruSeq Stranded mRNA kit and sequenced as 100 bp single-end reads in one flow cell lane on a NovaSeq 6000 sequencer.