title: GSM8074520: BPK mammary tumor formed after 24x injections of 4NQO; Mus musculus; RNA-Seq
library_construction_protocol: Removed post-mortem glands were divested of lymph nodes and processed into single cells. Glands were mechanically dissociated in 1x PBS (Gibco Cat. No. 10010023) into a pulpous mass before being enzymatically digested with 2mg/ml collagenase XI (Milipore-Sigma Cat. No. C9407) and 0.1 mg/ml hyaluronidase (Milipore-Sigma Cat. No. H3506). Cell suspensions were further digested by 100ug/mL DNAse I (StemCell Tech Cat. No. 07900) and 5U/mL Dispase II (Milipore-Sigma Cat. No. D4693) to generate single cell suspensions. Suspension was filtered through 40mm filter (puriSelect Cat. No. 43-50040-01) and washed in PBS, counted, and submitted for scRNA-seq Single cell librarier were generated according to the manufacturer's instructions for 10x Genomics single cell 3' v2 chemistry protocol. 10x Genomics scRNA-seq library preparation.