title: GSM6971593: YF, batch2, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: ESLAM HSCs were sorted as CD45+EPCR+CD150+CD48- and transduced with lentivirus containing STAT5B-YF, STAT5B-WT and EV in SCF and IL-11 cultures. Five days after infection, cells were sorted for viability (DAPI-, Thermo Fisher) and green fluorescent protein (GFP) expression. The cells were processed with a Chromium Controller according to the Chromium NextGEM Single Cell 3' GeneExpression v3.1 protocol. The libraries were pooled and sequenced on a Novaseq 6000