title: WGS of soil from Control
design: We first extracted DNA from ~0.2 g of soil using the DNeasy PowerSoil Pro Kit (Qiagen, Germantown, MD). DNA was sequenced at the Michigan State University Genomics Research Technology Support Facility. Libraries were prepared using the Illumina TruSeq Nano DNA Library Prep with IDT for Illumina TruSeq Unique Dual Index adapters following manufacturers recommendations. Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. The libraries were pooled in equimolar amounts and quantified using the Invitrogen Collibri Quantification qPCR kit and loaded onto one lane of an Illumina NovaSeq S4 flow cell for each year of analysis. Sequencing was performed in a 2x150bp paired end format using a NovaSeq 6000 v1.5 300 cycle reagent kit.