title: GSM7794843: NHBE IAV-WT scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: NHBE cells were infected with IAV-WT at MOI of 3 for 12 hours. At 12 hpi, dead cells were washed off the plate, and the live cells were trypsinized and washed in PBS and centrifuged at 300xg for 5 minutes. The cells were counted to ensure >90% viability and concentrated to 2.12x10E6 cells per mL. A total of 10 μL of cells were hence loaded into PIPseq T20 3' Single Cell RNA kit v4.0 (Fluent BioSciences, (Clark et al., 2023) reaction tube to be processed, intended to capture 10,000 cells. Following the manufacturer's protocol, the transcripts from cells were barcoded, and processed for Illumina sequencing on NextSeq2000 platform using P3-100 cycle kit. A total of 267 million reads were assigned to the sample. Fluent BioSciences PIPseq 3' single cell RNA-seq