title: GSM8441787: Pig oocyte, scBS-Seq, in-vitro, cell 21; Sus scrofa; OTHER
library_construction_protocol: Matured oocytes were individually lysed and flash-frozen in 5 μL of RLT Plus buffer (Qiagen) at −80°C until further use. Both DNA and RNA from lysate were separated using the G&T-seq and Smartseq2 protocol (Angermueller et al., 2016). Magnetic beads (MyOne C1, Life Technologies) were washed and then annealed to oligo dTs. These were employed to capture polyadenylated mRNA from cell lysates Single-cell BS-seq libraries were prepared based on a previous method described by Clark et al. (2017).  The lysate containing the DNA was purified using a 0.9:1 ratio of Ampure XP Beads (Beckman Coulter) and eluted into 10 μl of water to use for scBS-seq library preparation. DNA purified from single cells was treated using the EZ Methylation Direct Kit (Zymo) for bisulfite-conversion following the manufacturer's instructions