title: GSM7113376: EpiSC azd; Mus musculus; RNA-Seq
library_construction_protocol: Single cell transcriptomes were generated with the 10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 according to the manufacturer's instructions. Briefly, cells were differentiated for three days, detached with Accutase and counted. The cell suspension was diluted to a concentration of 900 cells/μl, to recover around 2000 cells per sample. Cell suspensions were loaded on a Chromium Controller (10x Genomics) to partition cells with gel beads in emulsion.  Reverse transcription, cDNA recovery and amplification, and sequencing library construction were performed according to manufacturer's instructions (10x Genomics ChromiumNextGEMSingleCell_v3.1_Rev_D). We chose 12 PCR cycles for cDNA amplification, and 10 PCR cycles for index PCR.