title: 16S sequencing of Lucilia cuprina microbiome: G4 H2O adult males rep 3
design: The V4 region of the 16S rRNA gene was amplified using 16S V4: 515F-806R primers, with attached barcode. Forward primer: GTGYCAGCMGCCGCGGTAA. Reverse primer: GGACTACNVGGGTWTCTAAT. Sequencing was performed on an Illumina NovaSeq 6000 platform. Paired-end reads were assigned to samples based on their unique barcode. Barcode and primer removal, demultiplexing, and quality filtering were performed by Novogene Corporation (Sacramento, CA, USA).