title: GSM7794711: Hypertrophic scar, scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: The skin tissue were excised and immediately transferred to a 50-ml centrifuge tube and washed with precooled PBS at 4 °C. The tissues were placed in a precooled dish on ice, cut into 2 to 4-mm pieces, and then all transferred to an Eppendorf tube containing an enzyme mix [Collagenase I (1 mg/ml) + Collagenase IV (0.5 mg/ml) + Collagenase V (0.5 mg/ml) (Solarbio) + Collagenase-neutral protease (Roche) + DNase I (Solarbio)]. A gentleMACSTM Octo Dissociates with Heaters was used for 4 h for dissociation and digestion. Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 3ʹ Reagent Kits v3 (10x Genomics) , according to the manufacturer's instructions.