title: 16S of cultivated biocrust: greenhouse cultivation, no inoculum (control), time 1, sample 11
design: DNA was extracted from soil samples using the QIAGEN Power Soil kit. We PCR amplified the 16S gene using 515f/806r primers with Illumina barcodes. We confirmed amplification with gel electrophoresis and then cleaned up the library with the SequalPrep Normalization kit.