title: GSM8372111: RUES02, TIRN+iDUX4 (LIF-3i + dox), RNAseq, batch 01, replicate 002; Homo sapiens; RNA-Seq
library_construction_protocol: RUES02 hESC were prepared in E8 (primed; primed +iDUX4 after 24 hours supplementation with 2mg/mL doxycycline) or LIF-3i/MEF (TIRN; TIRN +iDUX4 after 24 hours supplementation with 2mg/mL doxycycline). Cells were gently collected using a mixture 1:1 Versene/Accutase (5 min) and pass through a 70um cell strainer. PolyA mRNA libraries were prepared  using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions. PolyA mRNA libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB#E7490) following manufacturer's protocols .