title: GSM8037015: PBS_HUB; Mus musculus; RNA-Seq
library_construction_protocol: Meningeal rostral-rhinal hubs from twelve 15-week-old naïve or d8 VSV-infected mice B6 mice transcardially perfused with 20 ml of ice-cold PBS were carefully collected under a dissection microscope. Upon isolation, meningeal hub samples were pooled for each experimental group and dissociated with a mouse Tumor Dissociation Kit (Miltenyi Biotec) using the gentleMACS Octo Dissociator (Miltenyi Biotec). After dissociation, the samples were then filtered through 100µm meshes and washed with 2% fetal bovine serum in RPMI and resuspended in 0.05% BSA PBS solution. Cell number and viability were checked with the Luna-FX7 (Logos Bio). cDNA libraries were prepared from single-cell suspensions following the 10x Genomics 3′ v3 protocol using either single indices or dual indicies (www.10xgenomics.com) and sequenced on a Illumina NextSeq 550 (www.illumina.com).