title: GSM6585474: Sample_89DI; Mus musculus; RNA-Seq
library_construction_protocol: Samples were prepared for extraction using the standard protocol for lipid-rich samples described in the QIAsymphony RNA Handbook (issued November 2020).  Tissue disruption and homogenization was carried out using a Qiagen Tissuelyser II.  RNA extraction was performed using a QIAsymphony SP (protocol: RNA_CT_800_V7, kit: QIAsymphony RNA Kit (192), catalog no. 931636).  RNA was eluted into 100 µL of RNase-free water and analyzed using an Agilent 4200 TapeStation. Libraries were prepared by Illumina TruSeq Stranded mRNA Library Preparation Kit protocol (catalog no. 20020595).  The libraries were analyzed by Agilent 4200 TapeStation, and pooled.  The pooled libraries were loaded on a NextSeq 500/550 High Output v2.5 run with a 75-cycle sequencing module (catalog no. 20024906) to generate paired-end reads.  The demultiplexing of the reads was performed with bcl2fastq version 2.20.0