title: 16S rRNA gene sequence of coral reef bacterioplankton community: Coral 1 - 4 days
design: Coral reef bacterioplankton communities were enriched with high molecular weight (HMW, > 1 kDa) carbohydrates released by macroalgae or corals, and HMW seawater extracts were used as controls. Triplicate bacterioplankton incubations with added HMW carbohydrates were left in the dark at ambient temperatures for four days. Samples for amplicon library sequencing were collected before the start of the experiment ("Ambient"), at the start, after 6 and 12 hours, and after 1 and 4 days. DNA was extracted from polyethersulfone filters (0.2 m pore size) which were used to filter seawater samples (volume 0.2 - 1.2 L) using a modified version of the NucleoSpin Tissue DNA extraction kit. The 16S rRNA gene was amplified following protocols of the Earth Microbiome Project with minor changes. Amplicons were cleaned and normalized to equimolar concentrations, and pooled and sequenced on the Illumina MiSeq platform (600 cycle V3 chemistry). Sequences were demultiplexed using a custom probabilistic script.