title: GSM8296528: PBS-2 [S_276]; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were obtained by cell sorting, staining for lineage markers (Lin), cKit/CD117, and Annexin V.  Prior to sorting, DAPI was added for viability.  Cells stained as Lin-cKit+Annexin V-DAPI- were sorted and stored at -80ºC until processing. RNA was isolated using a Total RNA Purification Kit (Norgen Biotek Corp). The quantity and quality of the RNAs were evaluated using Agilent RNA 6000 Pico Chips (Agilent Technologies, Cat.# 5067-1513). Sequencing libraries were prepared using “NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®” (New England Biolabs, Cat # E6420S) and “NEBNext Multiplex Oligos for Illumina (Index Primers 1-12)” (New England Biolabs, Cat # E7335S), following the corresponding Instruction manual (Version 5.0_5/20)