title: GSM6585552: gRNA GFP-; Homo sapiens; OTHER
library_construction_protocol: gRNA: Genomic DNA from sorted cell pellets was extracted using the QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (ThermoScientific; Q32850) following the manufacturer's guidelines. A quantity of gDNA covering 1000X representation of gRNAs was PCR amplified to add Illumina adapters and multiplexing barcodes. gRNA: Amplicons were quantified by Qubit and Bioanalyzer (Agilent) and sequenced on the Illumina HiSeq 2500 platform.