title: GSM6582703: ST-HSC (SLX.12953.i714_i517); Homo sapiens; RNA-Seq
library_construction_protocol: Single cells were directly sorted in lysis buffer according to Smart-Seq2 protocol (Picelli et al., Nat protocol, 2014). A lysis buffer was prepared of 20U/l SUPER-In RNase inhibitor (Thermo Fisher Scientific) and 0.4% Triton-X100 (ratio of 1:19). The lysis buffer was added to a mix of DTT (Thermo Fisher Scientific) (final concentration of 0.57mM), dNTPs (Invitrogen) (final concentration 0.11mM) and nuclease free water (Thermo Fisher Scientific) and 4ul aliquoted per well in a 96 well PCR plate and stored at -80°C. libraries were prepared for sequencing using standard Illumina protocols