title: GSM8073994: Heart, Isoproterenol and Minocycline, 842; Mus musculus; RNA-Seq
library_construction_protocol: Qiagen RNEasy Lipid Tissue Mini Kit (Cat # 74804) A cDNA library constructed by technology from the pooled RNA from the left ventricle of mice was sequenced run with Illumina Novaseq TM 6000 sequence platform. Using the Illuminapaired-end RNA-seq approach, we sequenced the transcriptome, generating a total of millon 2 x 150 bp paired-endreads.The RNA-Seq analyses module included in the CLC Genomics Workbench v. 21.0.3 (Qiagen®) (22) was used for the analyzing transcriptome data. Briefly, the compressed Fastq files were extracted, trimmed and filtered using the software's default parameters. The Workbench uses modified-Mott trimming algorithm for trimming sequence ends with the following trim parameters used: trim using quality scores, yes; quality limit, 0.05; trim ambiguous nucleotides, yes; maximum number of ambiguities, 2; automatic read-through adapter trimming, yes, and trim ambiguous nucleotides, yes. A total of 784,251,576 sequences were processed and the total nucleotides in the datasets were 108,840,542,046.