title: GSM8073461: Day 0, Prox1 eGFP; Mus musculus; RNA-Seq
library_construction_protocol: Cells from the excised tissue were isolated based on previously described methods . Briefly, tissue was digested for 45 min in 0.3% Type 1 Collagenase and 0.4% Dispase II (Gibco) in Roswell Park Memorial Institute (RPMI) medium at 37 °C under constant agitation at 120 rpm. Digestions were quenched with 10% FBS RPMI and filtered through 40μm sterile strainers. Samples were run on a FACSAria II for cell sorting to separate the Prox1-expressing population for further scRNA-seq analysis. Single Cell 3' v3