title: GSM6503089: A549, NK resistant; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were seeded with 1.5 ×10^5 cells per well in 12-well plates. After 72 hrs, the cells were lysed using 700 µL TRIzolTM Reagent (Life Technologies) and incubated for 5 min at room temperature on a plate rocker. RNA was then isolated and purified using the Total RNA Purification kit (Norgen) under the manufacturer's instructions. The purified RNA samples were then submitted to the Advanced Genomics Core at the University of Michigan for library prep and next-generation sequencing. Input RNA is normalized to 200ng per sample. The RNA library was prepared with Poly(A) mRNA Magnetic Isolation Module (New England BioLabs), NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs), and NEBNext Multiplex Oligos for Illumina Unique dual (New England BioLabs).