title: GSM7795355: Pancreatic islets, SPF, P15 [scRNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: Purification from acinar tissue was achieved by several rounds of hand picking. Islets from 20 animals in each group were pooled and digested using Gibco Enzyme Free Cell Dissociation Buffer at 37 C for 15 minutes. Samples were vortexed every 5 minutes throughout the incubation. Washed and resuspended cells from the islet dissociation were run through a CD45 positive selection column (Miltenyi Biotec, CD45 MicroBeads mouse) followed by a dead cell removal kit (Miltenyi Biotec) to isolate live cells. Next GEM Single Cell 3' Gene Expression Library Prep version 3.1 with UDI (10X Genomics).