title: V9 region from 18S rRNA
design: To determine the microbiota present in the samples, the 18S-V9 region (primers Euk_1391f and EukBr) was amplified by PCR with Illumina adapters. Indices were added to the final products according to the protocol recommended by Illumina. Finally, the samples were quantified in Qubit and mixed in an equimolar pool to be sequenced in the Illumina Miniseq equipment under standard conditions (300 cycles, 2X150).