title: GSM7866228: hESC cells, WT-KRT18P37 rep1; Homo sapiens; RNA-Seq
library_construction_protocol: hESC was digested into single-cell using accutase and centrifuged at 1000 r.p.m. for 5 min. 1mL Trizol was added to mix the cell pellets, and 200μL chloroform was added for shaking violently. After centrifuging at 12000 r.p.m., 4 ° C for 15min, the upper water phase was absorbed, and added with equal volume isopropyl alcohol. RNA was precipitated by centrifugation at 12000 r.p.m., 4 ° C for 15min and cleaned with 75% ethanol then dissolved in RNase-free water. RNA concentration was measured using nanodrop. Stranded sequencing library preparation by VAHTS Universal V6 RNA-seq Library Prep Kit for  Illumina(NR604-01/02) according to manufacturer's instructions.