title: GSM6925723: radiation 1 week, 1 Gy rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Immediately following irradiation, total RNA was isolated from MSC using the RNeasy Mini Kit with on-column DNase digestion (Qiagen) according to manufacturer's protocol. RNA was quantified spectroscopically (ND-1000 Spectrophotometer, Nano-Drop, Wilmington, DE, USA). Sequencing libraries were generated using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Samples were sequenced using a NovaSeq 6000 instrument (Illumina, San Diego, CA).