title: GSM6585700: Liver colorectal cancer RRBS, patient #270; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was digested with MspI enzyme followed by end-repair and ligation of Illumina TruSeq sequencing adaptors. Fragments were then size-selected and bisulfite-converted using the EZ DNA methylation kit prior to 16-18 rounds of PCR amplification. The quality and size distribution of the libraries was determined using a bioanalyser and libraries were sequenced on an Illumina HiSeq2500 machine (100 bp reads, single-ended). Base-calling of the reads was performed with Illumina Real Time Analyzer (RTA) software.