title: Illumina NovaSeq 6000 paired end sequencing; Transcriptomic profile of a Clostridioides difficile rsbW deletion mutant compared to parental R20291 strain
design: Transcriptomic profile of a Clostridioides difficile rsbW deletion mutant compared to parental R20291 strain
library_construction_protocol: Early stationary (10h) planktonic cultures grown in BHI-S media in anaerobic conditions. 5 mL of culture was resuspended in 5 mL ice-cold LETS buffer. Cells were lysed using Fast-prep 24 instrument (MP Bioscience). Trizol (Ambicon, USA) was used for RNA extraction in phenol-chloroform extraction. Total RNA was treated with DNase, rRNA removal, cDNA reverse transcription and library preparation. rRNA removal, cDNA reverse transcription and library preparation was conducted by Novogene (China).