title: GSM7831844: HepG2 m6A 1; Homo sapiens; RIP-Seq
library_construction_protocol: total cellular RNA extracted from cells were fragmented by RNA fragmentation reagents (Thermo, AM8740). Fragmented RNA was incubated with an anti-m6A antibody (#202003, Synaptic Systems) or IgG IP Grade Rabbit polyclonal antibody at 4°C for 6 h in 500 μl of NT2 buffer (200 mM NaCl, 50 mM HEPES pH 7.6, 2 mM EDTA, 0.05% NP-40, 0.5 mM DTT, 200 U ml–1 RNase inhibitor). The purified RNA fragments were used to construct libraries with the SMARTer Stranded Total RNA-seq Kit v2 (Takara)