title: GSM8213639: FACS Microglia, LPS Injected, WT, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells with an miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions. Library preparation and sequencing was performed at the NGS core facility at the Research Institute for Microbial Diseases of Osaka University. Full-length cDNA was generated using a SMART-Seq HT Kit (Takara Bio) according to the manufacturer's instructions. An Illumina library was prepared using a Nextera DNA Library Preparation Kit (Illumina) according to SMARTer kit instructions.