title: Nanopore reads of Human astrovirus
design: Extracted RNA was subjected to the synthesis of cDNA using NEBNext Ultra II RNA First Strand Synthesis Module (New England Biolabs, USA) and NEBNext Ultra II Non-Directional RNA Second Strand Synthesis Module (New England Biolabs, USA). cDNA aliquots (10 L) were mixed and enriched with AMPure XP beads (Beckman Coulter, A63881) by 1.8 volume content. A 15-L enriched cDNA was used for library preparation. For the MinION (Oxford Nanopore Technologies), we used the Rapid Barcoding Kit (SQK-RBK004) for library preparation and R9.4 flow cell (FLO-MIN106) for sequencing according to the manufacturer's protocol.