title: GSM8440609: Priming, GFPneg, H3K27me3, rep1; Homo sapiens; OTHER
library_construction_protocol: Cut&Run-seq was performed using CUTANA v3 kit (Epicypher) according to the manufacturer's protocol and with mild crosslinking (1 min incubation with 1% formaldehyde at room temperature). Sequencing libraries were performed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the published protocol (Nan Liu 2021. Library Prep for CUT&RUN with NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645). protocols.io https://dx.doi.org/10.17504/protocols.io.bagaibse). The samples were multiplexed with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2) (NEB). Size selection steps were performed with Ampure XP beads (Beckman Coulter) and adjusted for nucleosomal DNA fragment size (150bp, excluding adapters).