title: fungal metataxonomics 26S rRNA from fermented vegetable;  fermentation of Violet cabbage collected the day 9 Mar 2021; sample code (VV-B-7)
design: The DNA was extracted using Master Pure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA). DNA quality and concentration was evaluated with NanoDrop spectrophotometer (Thermo Scientific, Milan, Italy). Library of 26S rRNA of fungal DNA was constructed using MiSeq Reagent Kit V3 (2250 bp) (Illumina, San Diego, USA).PCR products were purified by means of an Agencourt AMPure kit (Beckman Coulter, Milan, Italy), and the resulting products were tagged with sequencing adapters using the Nextera XT library preparation kit (Illumina Inc, San Diego, CA). Sequencing was performed using a MiSeq Illumina instrument (Illumina) with V3 chemistry, which generated 2X250 bp paired-end reads. MiSeq Control Software, V2.3.0.3, RTA, v1.18.42.0, and CASAVA, v1.8.2, were used for the base-calling and Illumina barcode demultiplexing processes.