title: GSM8295244: SI_ARBO_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Cell lines were seeded in 100 mm tissue culture dishes (Corning, NY, USA, Cat. 430167) at a density of 2.5x106 for HeLa and 3x106 for SiHa cells, both cell lines were incubated under previous conditions overnight. Subsequently, cells were treated with a 1:5,000 dilution of AEO or DMSO alone (vehicle control). After 4 hours of incubation, the cells were harvested for RNA extraction using the column-based extraction and purification system Zymo Quick-RNA MiniPrep (Zymo Research Corp., Irvine, CA, USA) following manufacturer's instructions. The obtained RNA was quantified by absorbance at 260/280 nm and stored at -80°C until further use First, total RNA is fragmented into 150 pb segmentes. Second, Reverse transcription is achive to obtain cDNA. Third, ligation of adapters at the ends.