title: Litopenaeus vannamei transcriptome
design: Healthy adult shrimps were obtained from the Rudong base of the Institute of Oceanology & Marine Fisheries, Jiangsu, Nantong, China. Shrimp was temporarily cultured in the indoor tank for 7 days before the experiment. During this period, 1/3 volume of filtered, precipitated and fully aerated seawater (24C  0.5C) was replaced daily, bait was fed regularly, and dead shrimp, residual bait and excreta were cleaned up in time. 300 of neatly sized and healthy shrimps were selected and placed into three 1000 L PVC drums (experimental conditions were consistent with those during the temporary storage period) after acclimatization, and the experiment was started after 2 d of stabilization. The starting temperature of the experiment was set at 24C and was automatically cooled down by a chiller at a rate of 2C /2 h. 6 shrimp (3 repetitions) were randomly selected from 24C(CK), 18C(T-2) and 12C(T-4) groups respectively, and the same samples were shared. All samples were rapidly frozen in liquid nitrogen and stored in a refrigerator at -80C.