title: GSM8074016: skin, E14.5 Wntless mutant; Mus musculus; RNA-Seq
library_construction_protocol: Embryonic dorsolateral/flank skin was micro-dissected from E14.5 littermate control and mutant embryos and dissociated into a single-cell suspension using 0.25% trypsin (Gibco, Life Technologies) for 20 minutes at 37◦C. After genotyping, 2-3 embryos were pooled by condition. Single-cell suspensions were then stained with DAPI (Fisher Scientific, NBP2-31156) just prior to fluorescence-activated cell sorting. Chromium Single cell 3' GEM Library and Gel Bead Kit v3.1 (PN-1000121) was used according to the manufacturer's instructions in the Chromium Single Cell 3' Reagents Kits V3.1 User Guide. After cDNA libraries were created, they were subjected to Novaseq 6000 (Illumina) sequencing. For each scRNA-seq experiment, control and littermate mutant samples were prepared in parallel at the same time, pooled, and sequenced on the same lane.