title: GSM6974018: Day14, libraryB, GFP_to_mCherry_low, bio_rep1; Homo sapiens; OTHER
library_construction_protocol: For CRISPR screen, genomic DNA was extracted using the Zymo Research Quick-gDNA Midiprep Plus kit (D4075); For eCLIP, RNAs were extracted using the RBP-eCLIP kit (ECLIPSEbio) according to the manufacturer's instructions. For CRISPR screen, sgRNA sequences were amplified by PCR following the previously described procedure (Joung et al, 2017, Nat Protoc); For eCLIP, libraries were constructed using the RBP-eCLIP kit (ECLIPSEbio) according to the manufacturer's instructions.