title: GSM8262378: OV81_DL78_2; Homo sapiens; RNA-Seq
library_construction_protocol: Media, PBS wash, and trypsinized cells were collected in a 15mL conical and centrifuged at 300g for 5 minutes. The supernantant was aspirated and the pellet was resuspended in a PBS wash. After a second centrifuge, the PBS was removed and the pellet was stored at -80ºC. RNA was extracted using the RNeasy Plus Kit (Qiagen #74134) according to the manufacturer's protocol. The libraries were barcoded, pooled, and sequenced using paired-end, 151 bp sequencing at the University of Michigan Advanced Genomics Core.