title: GSM8372126: A11_XC01_M07_T002; Mus musculus; RNA-Seq
library_construction_protocol: Collect the pieces into a C tube and add digestive enzyme mix（Miltenyi Biotec-human/mouse Tumor Dissociation Kit,）, fill with cell culture medium up to 5 mL. Incubate the tissue soup in a shaking incubator at 37℃ for 1 hour. Filter the dissociated tissue through the 70 μm cell strainer. Collect the cells by centrifugation at 300 x g for 5 minutes at 2-8°C. Aspirate the supernatant and resuspend the cells in CSB and count cell number. The cells are ready for further staining library was constructed accoding to the manufactor's  instructions (MobiCube 3' RNA v2.0).