title: GSM8526932: PD-1 KO naive, Tcf7+, S4; Mus musculus; RNA-Seq
library_construction_protocol: Sorted cells were lysed and stored in Trizol before extraction of total cellular RNA using the Direct-zol RNA MiniPrep kit (Zymo Research). RNA quality was assessed on a Fragment Analyzer (Agilent Technologies) and all RNAs had an RQN ≥ 8.7. From 50 ng total RNA, mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module. RNA-seq libraries were then prepared from the mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina NEBNext Ultra II RNA Library Prep Kit (non-strand specific library preparation)