title: GSM7865713: TK_20181007_Bud_tree92; Prunus yedoensis; RNA-Seq
library_construction_protocol: Total RNA of leaf sample was extracted in accordance with the method described in a previous study. Total RNA of bud sample was extracted using the PureLink™ Plant RNA Reagent (Thermo Fisher Scientific, Waltham, MA, USA, DNase-processed using the TURBO DNA-free kit™ (Thermo Fisher Scientific, Waltham, MA, USA) according to their protocols, and purified using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) according to its RNA cleanup protocol. Five to six micrograms of RNA extracted from the leaves of each sample were sent to Hokkaido System Science Co., Ltd., where a cDNA library was prepared with a NovaSeq 6000 S4 Reagent Kit, and 151 base paired-end transcriptome sequencing of each sample was conducted using an Illumina NovaSeq6000 sequencer (Illumina, San Diego, CA, USA).