title: GSM8372468: Colon_naive_STAT6vt_control_rep3; Mus musculus; RNA-Seq
library_construction_protocol: We collected colon tissue in RNA protect (Qiagen) and isolated RNA withe the Rneasy Mini Kit (Qiagen). DNA was digested with the DNAse1 Amplification Kit (Merck) and RNA integrity measured at an Agilent tape station (Agilent). RNA was sequenced by Illumina sequencing (Illumina). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.