title: GSM8296594: Hematopoetic_CD8T_21; Mus musculus; OTHER
library_construction_protocol: Stained slides were loaded and scanned onto a GeoMx instrument.Seven Regions of interest (ROI) were selected based on the CD45 and CD8 fluorescent staining and auto-segmentation algorithm. RNA was isolated using Agilent RNA isolation kit with manufactured recommended protocol. Photocleaved oligonucleotides from the ROI were collected and products were processed and sequenced on an Illumina NextSeq 2000. The ROI were selected based on the positive staining of CD8 and CD45