title: GSM8210480: Liver healthy, biopsy, rep 3; Marmota monax; RNA-Seq
library_construction_protocol: Woodchuck livers were dissociated according to a previously described 2-step collagenase perfusion protocol https://doi.org/10.1038/s41467-018-06318-7. For PBMCs, whole blood was collected in EDTA tubes. Plasma was collected from these tubes via 1000 x g spin for 10 minutes. Remaining blood was diluted using a 1:1 ratio of 1X PBS. This mixture was carefully layered on a Ficoll density gradient. Tubes containing layered blood were centrifuged at 400 x g for 20 minutes without brakes. The buffy coat layer was isolated using a Pasteur pipette and washed with 1X PBS. Single-cell samples were sequenced with 10X Genomics SingleCell 3' v2 chemistry according to manufacturer's instructions.