title: GSM6970323: Brevibacillus brevis, Control 3; Brevibacillus brevis; RNA-Seq
library_construction_protocol: Total RNA integrity was assessed using the Agilent 4200 TapeStation (Agilent Technologies, Santa Clara, CA, USA) and RiboGreen dye. Libraries for RNA-Seq were obtained from total RNA using the TruSeq Stranded mRNA Sample Preparation Kit. Poly A+ RNA was purified by AMPure XP beads and fragmented to 200 ~ 700 bp. cDNA was synthesized with reverse transcriptase (SuperScript II) and random primers. The strand-specific RNA-Seq library was obtained by the dUTP second-strand marking method. Double-stranded DNA was ligated to barcoded TruSeq adapters for library preparation. PCR was carried out on the size of fragments selected for library amplification. Libraries were sequenced on a NovaSeq 6000 System (Illumina) using a paired-end run (2 × 101 bases).