title: GSM8262011: LNCaP_FV_NE_s_2_15j; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction was performed directly from cell cultures with miRNeasy kit according to the manufacture' instructions including the DNase treatment step (Qiagen). Trueseq stranded Total RNA-seq protocol with Ribo-zero depletion of rRNA from 500 ng of the total RNA extract sample