title: 16S rRNA sequencing of colon contents: colon of male rats
design: Correct biological sequences obtained by sequence de-noising are often referred to as Amplicon Sequence Variants (ASVs). The taxonomy of ASVs was analyzed by Qiime2 (Version 2020.2) against the 16S rRNA database (Silva138/16s_bacteria) using a confidence threshold of 0.7.