title: Soil Mycobiome SS16ITS1
design: DNA was extracted from soil using FastDNA Spin kit for soil (MP Biomedicals, Solon, OH, USA), Amplification of both ITS1 and ITS2 regions was performed, PCR reactions were carried out in triplicates for each primers set, and for each annealing temperature (55 and 52C). Amplicons were then pooled together. Next-Generation Sequencing was performed using the Illumina MiSeq Sequencer (Illumina, Inc., CA, USA).