title: GSM7866386: KRAS,PI3KCAH1047R, GFP tumor positive whole brain 1; Danio rerio; RNA-Seq
library_construction_protocol: Dissected brains or sorted cells were immediately put into Trizol. RNA was then extracted and purified utilizing Monarch RNA Cleanup Kit following manufacturer's recommendations Sequence ready polyA-enriched libraries were prepared using the NEB Ultra II Directional mRNA prep kit for Illumina, according to manufacturer's recommendations