title: RNA-Seq of gametophytes on the 6th day during gametogenesis -2
design: Experiment procedure: 1. Sample collection, 2. DNA extraction and polymerase chain reaction, 3. Illumina Hiseq 2000 sequencingStatistical and bioinformatics analysis: 1. Filtering, mapping, and quantification, 2. Identification of differentially expressed genes and transcription factors, 3. Identification of alternative splicing events 4. Trend analysis of gene expression patterns