title: GSM6974560: Liver macrophages, vavCre transplanted, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Animals were sacrificed at 11 weeks of diet. Livers were perfused with PBS and processed into single cell suspension by enzymatic and physical dissociation. Hepatic macrophages were sorted using fluorescently labeled antibodies. RNA was isolated using Rneasy micro kit (Qiagen). 0.2 ug of total RNA was used for the construction of sequencing libraries. cDNA libraries for RNA-seq were prepared using NEBNext Single Cell/Low Input RNA Library Prep Kit following manufacturer's protocols. Pooled libraries were sequenced using Illumina NovaSeq 6000.