title: GSM7359785: HEK293T cells, STAU2_IN_1; Homo sapiens; RIP-Seq
library_construction_protocol: Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) and UPF1:RNA complexes were immunoprecipitated with antibody RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)