title: GSM7795579: control siRNA bio rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the miRNAeasy kit (Qiagen, 217084) according to the manufacturer's instructions. RNA sequencing was performed in the Genomic Shared Resource at The Ohio State University using NEBNext® UltraTM II Directional RNA Library Prep Kit for Illumina, NEBNext rRNA Depletion Kit, NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs. 100 ng total RNA (quantified using Qubit Fluorometer) was used as library input. Fragmentation time was 10 min and PCR was done 11x. Libraries were sequenced with Novaseq SP Pair_end 150 bp format.