title: GSM7744893: 3106Endo-IFNT3; Bos taurus; RNA-Seq
library_construction_protocol: Tissue samples were collected at the end of the treatment schedule, 12 hours after last infusion (8:30 am of day 17 or day 20). Animals received caudal epidural anesthesia and a sterilized custom-made mini-Tischler biopsy forceps with a 22 inch shaft length, 4 mm shaft diameter, and 4x8 mm bite size (Aries surgical, Davis CA) was passed through the cervix for collection of 1 endometrial biopsy. The biopsies were immediately placed into sterilized cryotubes and snap-frozen in liquid nitrogen. RNA was isolated from one frozen endometrial biopsy (~50-60 mg) following homogenization in 800 μL of TRIzol reagent (Life Technologies). Total RNA was purified using RNA Clean and Concentrator kit (Zymo Research) to eliminate contaminating DNA and reagents. Endometrial RNA samples were shipped on dry ice to BGI laboratory (Beijing Genomics Institute, Shenzhen, China) where RNA quality and quantity were assessed using Agilent 2100 Bioanalyzer (Agilent Technologies). All samples met the quality criteria for RNA sequencing, showing RNA integrity number (RIN) between 8 and 9.9 values. Preparation of mRNA library and sequencing were performed following BGI DNBSEQ technology platform with paired end 100 bp (PE100).