title: GSM7113349: 3LWbrain, 12xWT, M, rep3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 25 brains were dissected from wandering 3rd instar larvae and homogenized in Trizol solution. RNA was isolated from the Trizol aqueous phase using the Zymo RNA Clean and Concentrator-5 kit (Genesee Scientific #11-352) including treatment with DNAse I, as per the manufacturer's instructions. Libraries were prepared from polyA selected RNA using the KAPA stranded mRNA kit (Roche # 07962207001)