title: GSM6503033: BS1_IP, rep1, m6A-seq; Oryza sativa; RIP-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent according to the manufacture's protocol. Poly (A) mRNA was purified  using poly-T oligo attached magnetic beads (Invitrogen) and then fragmented into approximately ~100-nt-long oligonucleotides using Magnesium RNA Fragmentation Module.  Ten percent of fragmented mRNA was aliquoted as input control (Input). The remaining fragmented mRNA was used to collect  m6A-containing RNA fragments (immunoprecipitated, IP) by using m6A-specific antibody. Both IP and Input RNA fragments were used to construct strand-specific cDNA libraries according to the dUTP method.