title: GSM8164503: tumor_WT rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Tissue samples were digested with 1 mg/ml collagenase IV for 20 minutes. Cells were filtered with a 70-μm cell sieve and stained with Live/Dead fixable exclusion dye, followed by fluorochrome-conjugated antibodies in PBS with 2% fetal bovine serum (FBS) and 1mM EDTA. Live/CD45+/CD3+/CD4+/YFP+ cells were sorted. RNA was isolated using Qiagen RNeasy Plus Mini Kit. cDNA libraries were generated for poly-A selected RNA using the Illumina TruSeq mRNA Library Kit. 100 bp paired end reads were sequenced on the Illumina NextSeq 550 v2.5.