title: RD1W20B
design: Fecal swab samples were obtained from two distinct groups of domestic pigs: Sows and pigs at 20 weeks of age, sourced from nine different farms located in Alberta and Saskatchewan, Canada. Additionally, distal colon digesta samples were collected from wild pigs of reproductive age inhabiting regions including Melfort and Moose Mountain in Canada, as well as a location approximately 80 km north of Berlin, Germany. Genomic DNA extraction was performed from the fecal swab samples and approximately 180 mg of distal colon digesta samples using the DNeasy PowerSoil kit, following the manufacturers protocol. The Illumina 16S metagenomic sequencing library preparation protocol was used to prepare amplicon libraries targeting the V3-V4 region of the 16S rRNA gene. The pooled library was paired-end sequenced on an Illumina MiSeq platform using 2 x 300 cycles.