title: GSM8073967: Brain from mouse with vehicle #2; Mus musculus; RNA-Seq
library_construction_protocol: Mouse brains were transferred to RPMI-1640 medium, dissociated gently, and then, the protocol described by Korin et al. was followed. Target population (DAPI-CD45+CX3CR1-) was sorted into Lowbind Eppendorf tubes or cells were separated by magnetic beads using Mouse CX3CR1 positive selection to remove unwanted cells, and the negative fraction was collected in Lowbind Eppendorf tubes. Library was performed according to the manufacter's instructions scRNA-seq BD RhapsodyTM Immune response targeted panel