title: GSM8262555: A673 SUZ12ko clone2 Exp2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with NZY Total RNA Isolation kit (MB13402) following manufacturer's instructions. Total RNA samples (500ng) were processed with the "NEBNext Single Cell/Low Input RNA Library Prep" kit (NEB #E6420) by following manufacturer instructions. RNA Quality scores were 9.9 on average (range 9.1-10) when assayed on a PerkinElmer LabChip analyzer. Briefly, an oligo(dT) primed reverse transcription with a template switching reaction was followed by double stranded cDNA production by limited-cycle PCR.  Non-directional sequencing libraries were completed with the "NEBNext Ultra II FS DNA Library Prep Kit for Illumina" (NEB #E7805) and subsequently analyzed on an Illumina NextSeq 550 with v2.5 reagent kits following manufacturer's protocols.