title: GSM8527507: AML_2_CR_Untreated; Homo sapiens; RNA-Seq
library_construction_protocol: AML biopsies were checked for viability via Trypan Blue using a Neubauer chamber. If the viability was <70%, the cells were negatively sorted for viable cells using the Miltenyi Dead Cell Removal kit (Miltenyi Biotech). The cell concentration for each sample was adjusted to allow for a target output of ~10000 cells/sample. Single cells from all samples using the Chromium Controller were encapsulated into emulsion droplets (10x Genomics). The Chromium Single Cell 3' v2 Reagent Kit was used to construct the scRNA-Seq libraries, according to the manufacturer's protocol. Libraries were sequenced using the NovoSeq 6000 (Illumina).