title: GSM8494237: lung tissue transferred ALDHdim cells, rep1; Mus musculus; RNA-Seq
library_construction_protocol: The lung tissues of bleomycin-induced pulmonary fibrosis were homogenized using 1 mL TRIzol reagent (Life Technologies) and total RNA was extracted using the RNeasy Mini Kit (Qiagen). Libraries were prepared by BRB-Seq(Alpern D, et al.) method. Individual samples were reverse transcribed using dT primer with unique cell barcodes. Then replicate were mixed and amplified by PCR. 10nt of UMI followedby 9nt of cell barcode are in read1 and 81nt of RNA insert reads are in read2.