title: Total RNA-Seq of HCC1395 cell line
design: RNA input of 1microgram was used to generate the RNA-seq library using the New England BioLabs NEBNext Ultra II Directional RNA Library Prep for Illumina kit with rRNA Depletion module and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set 1) per manufacturers protocol. Paired-end sequencing was performed on the NovaSeq platform.