title: GSM6721197: 72h Treg T_R3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from cell pellets, using the RNeasy Mini Kit (Qiagen), including an on-column DNase digestion (Qiagen), according to manufacturer's instructions Strand-specific libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina Inc.,) according to the manufacturer's instructions. The libraries were analyzed on a 2100 Bioanalyzer using a 7500 chip (Agilent), diluted and pooled equimolar into a multiplex sequencing pool. The libraries were sequenced with 65 base single reads on a HiSeq2500 using V4 chemistry (Illumina Inc.,).