title: GSM7646190: RNAseq_MEPs_VhlcKO_1; Mus musculus; RNA-Seq
library_construction_protocol: MEPs (live, negative for F4/80, CD3, CD4, CD5, CD8, CD19, NK1.1, Ter119 and Gr1; CD45+ cKIT+ Sca1- CD34- CD16/32-) from pooled bone marrow of control and VhlcKO mice were isolated by flow cytometric sorting on the FACS Aria3 and stored in RLT lysis buffer (Qiagen, Redwood City, CA, USA) supplemented with β-mercaptoethanol at -80C until RNA isolation. Library construction was performed with the SMARTer® Stranded Total RNA-Seq Kit v3