title: Complete Genome Sequence of Extensively Drug-Resistant Elizabethkingia anophelis C08 Isolated from the Blood of a Patient with Bacteremia
design: 200μg genomic DNA was randomly fragmented by Covaris to an average size of 300-350bp. The fragments were treated with End Prep Enzyme Mix for end repairing, 5' Phosphorylation and 3' adenylated, to add adaptors to both ends. Size selection of Adaptor-ligated DNA was then performed by DNA Cleanup beads. Each sample was then amplified by PCR for 8 cycles using P5 and P7 primers, with both primers carrying sequences which can anneal with flowcell to perform bridge PCR and P7 primer carrying a six-base index allowing for multiplexing. The PCR products were cleaned up and validated using an Agilent 2100 Bioanalyzer. The qualified libraries were sequenced pair end PE150 on the illumina HiseqXten/Novaseq/MGI2000 System.