title: PacBio HiFi sequencing of Atlantic herring
design: Tissue was extracted on-site and immediately flash frozen in liquid nitrogen. High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing (CCS) mode to generate about 20 Gb of HiFi sequence data.