title: GSM8169600: Day-14-Total-RNA-MTR4-null-Cytoplasm-rep2; Mus musculus; RNA-Seq
library_construction_protocol: Oocytes were collected from indicated genotypes from mice of different stages. Then nucleus-cytoplasm separation were performed with micromanipulation. Nuclei and cytoplasm were collected separately. After sample collection,total RNA were extracted using TRIzol. Purified total RNA were firstly removed rRNAs using the Abclonal rRNA Depletion module, total RNA was used as input for library construction using the Abclonal Fast RNA-seq Lib Prep Kit V2. DNA fragments were selected,and the final sequencing libraries were constructed from 300–400 bp of the amplified cDNAs. Sequencing data were extracted on the Illumina HiSeq X Ten platform in the 150 bp paired-end mode.