title: GSM8411152: D1KO_rep2; Mus musculus; OTHER
library_construction_protocol: RNA was extracted with TriPure (Roche), according to the instructions of the SLAM–seq Kit (Lexogen). Alkylation of incorporated S4U nucleotides was performed from 4 µg labeled RNA. A sequencing library was prepared from 200 ng converted RNA with the QuantSeq 3' mRNA-Seq V2 Library Prep Kit with UDI (Lexogen) and sequenced on the Illumina NextSeq 2000 platform in SR100 mode.