title: V4 region from 16S rRNA
design: To determine the microbiota present in the samples, the 16S-V4 region (primers V4-515f and V4-806r) was amplified by PCR with Illumina adapters. Indices were added to the final products according to the protocol recommended by Illumina. Finally, the samples were quantified in Qubit and mixed in an equimolar pool to be sequenced in the Illumina Miniseq equipment under standard conditions (300 cycles, 2X150).