title: GSM8036072: PD-1+Tim-3- P14 T cells isolated from Cre-only mouse, replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were removed from infected mice, single-cell suspensions were prepared by mechanically disrupting tissue using double frosted slide, followed by red blood cell lysis with RBC Lysis Buffer , and stained with fluorescently labeled CD8, CD45.1, CD45.2, CD44 and PD-1 and Tim-3 antibodies. Then different subsets of Live CD8+T cells were sorted with help of fluorescence assisted cell sorter directly into lysis buffer. SmartSeq cDNA / Nextera XT libraries