title: GSM8410756: microglia, wild type, repeat2; Mus musculus; RNA-Seq
library_construction_protocol: At 9-month-old, Mice were anesthetized and transcardially perfused with ice-cold HBSS. The brains were isolated with the meninges removed, followed by dissection of the cortex and hippocampus, which were then minced and digested by 1% papain. Microglia were isolated from the resulting cell suspension via immunopanning with the rat-anti-mouse CD45 antibody (BD, 553076) and the secondary goat-anti-rat antibody (Jackson ImmunoResearch, 112-005-003), and directly lysed on the panning plate by Trizol (Thermo, 15596018), and stored at -80℃ before RNA extraction. Poly-A selected method was used to prepare the RNA-seq library. Sequencing was performed on the DNBSEQ platform with SE50 sequencing length