title: Metagenome of sediment core FW306
design: Genomic DNA was extracted from homogenized sediment using the QIAGEN PowerMax Soil kit (QIAGEN, USA) according to the manufacturers instructions with some modifications. For each replicate, 10 g of homogenized sediment was used, but split into two 5 g portions as input to the PowerMax Soil Kit. As a modification, after the addition of Solution C1 of the Soil Kit, the sample was subjected to a freeze-thaw and vortexed at max speed for 3.5 min after thawing. The samples were then incubated in a 65 C water bath for 30 min and mixed by inversion every 10 min. The rest of the DNA extraction was followed per the manufacturers instructions except during the elution step.  DNA was eluted using 5 mL of Solution C6 which had been pre-warmed to 55 C. The filter was incubated for a minute, then the DNA was eluted by centrifugation. This elution was repeated again to improveyield. DNA was concentrated by ethanol precipitation, using 1/10 volume of sodium acetate solution (3 M, pH 5.2, with 0.4 mg/mL glycogen) and 2.5 volumes of chilled 100% ethanol. Sequencing libraries were prepared using the Illumina DNA Prep Kit (Illumina, USA)per manufacturers instructions. Samples were sequenced by Novogene Corporation Inc. (California, USA) using 2 _ 150 bp on the IlluminaNovoseq 6000 (Illumina, USA).