title: RNAseq of isolated pancreatic islets from SUSD4 KO and control mice
design: RNAseq of isolated pancreatic islets from SUSD4 KO and control mice
library_construction_protocol: Pancreatic islets from mice were prepared by collagenase digestion of the exocrine pancreas. The islets were hand-picked in Hank's buffer (Sigma-Aldrich) with 1 mg/mL BSA. Total RNA for sequencing was isolated from mouse pancreatic islets using the miRNAeasy Kit (Qiagen, Hilden, Germany).  Illumina platform compatible sequencing libraries were constructed using NuGEN's Ovation Ultralow Library systems (NuGEN Technologies).