title: GSM7520225: Raji, P53 untreated control; Homo sapiens; ChIP-Seq
library_construction_protocol: CCL86 Raji and HTB96 U2OS cells were cultured for 80% confluency for ChIP-seq assay. Half of the cell cultures were treatment with doxorubicin and the other half remained untreated. We used 150 ng/ml concentration of doxorubicin for U2OS cells and 200 ng/ml for Raji cells. The multiple culture plates were pooled and then divided into six separate Petri dishes for determining cMYC and P53 genomic binding. Cells were fixed with 1% formaldehyde for 10 minutes, then quenched and washed two times before lysis. Chromatin was fragmented by sonication using ultrasonicator for 1.5 hours. Illumina HiSeq2500 platform in a 2x100bp paired-end configuration was used to obtain 35 million reads on average for each library sample