title: GSM6757512: cKO_vehicle2, replicate 2, ATACseq; Mus musculus; ATAC-seq
library_construction_protocol: Bone marrow (BM) cells from the femur and tibia were isolated and red blood cells were lysed. BM cells were cultured in DMEM-F12 media supplemented with 10%FBS and 20% supernatant derived from CMG14-12 (M-CSF producing) cell line for 5-7 days prior to experiments. BMDM polarization was assessed by cell morphology and later confirmed by cell surface expression of CD64, F4/80, CD11b, and CD11c Libraries from cells were prepared using the Illumina Tagment DNA Enzyme and Buffer Small Kit (Ilumina) according to manufacturer's protocol. Sequencing was performed using a third-party company (Novogene).