title: GSM6721276: OCIAML3-Abi; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the NucleoSpin RNA Plus Mini Kit (Macherey-Nagel) according to the manufacturer's recommendations. RNA sequencing libraries were prepared using 400 ng of total RNA using the Kapa mRNA Hyperprep kit (Roche) according to the manufacturer's recommendations and at 1/3rd reaction volume using 14 cycles of PCR. Libraries were analyzed using the Fragment Analyzer and quantified by qPCR prior to pooling and sequencing with the Illumina NextSeq 500 System generating 75 nt single end reads.