title: RNA-seq of Teredo navalis: Sections 3 "gill"
design: One individual of Teredo navalis was segmented into five equidistant sections across the body, clam shell end to gill (Tna1-Tna5) (Fig. 1a), and each section flash froze and stored at -80C. The samples are in the CIIMAR tissue animal collection. For each sectioned tissue, total RNA extraction was obtained using the NZY Total RNA Isolation kit (NZYTech, Lda. - Genes and Enzymes), according to the manufacturers instructions. The total RNA of the five samples was sent to Macrogen, Inc where Illumina paired-end strand-specific libraries (insert size of 250300 bp) were constructed and sequencing of 150 bp paired-end reads performed with HiSeq4000 platform.