title: Microbiome in a soil containing a heavy weathered crude oil biostimulated by mixing and nutrients
design: DNA was extracted using the Dneasy PowerSoil Kit according to manufacturer's (QIAGEN) protocol. DNA was sequenced using MiSeg instrument (Illumina) targeting the V4 hyper-variable region of the 16S rRNA gene for Bacteria and Archaea using Earth Microbiome Project primers 515F/806R.