title: GSM8527216: Oci-Ly8_H3K27me3_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quencing and cell lysis, chromatin was sonicated using Bioruptor Pico sonication device. ChIP-Seq libraries were prepared using the KAPA LTP Library preparation kit using the manufacturer's protocol with slight modifications. Briefly, after end‐repair and A‐tailing adaptor were ligated followed by Solid Phase Reversible Immobilization (SPRI) clean‐up. Libraries were amplified by PCR and fragments between 250 and 450 bp were selected using AMPure XP beads (Beckman Coulter, catalog number A63881). The libraries were analyzed for size and quantity of DNAs on a 2100 Bioanalyzer using a High Sensitivity DNA kit (Agilent), diluted and pooled in multiplex sequencing pools.