title: Long-read 16S: female urine microbiome Mock Community #2
design: To create the mock communities, five urinary strains of E. coli, one urinary strain of P. mirabilis, one urinary strain of S. epidermidis, and one urinary strain of E. faecalis were used. The E. coli strains represented different E. coli phylotypes, with two representatives from the B2 group. Each isolate was grown in their respective media; DNA was extracted using the Qiagen Blood & Tissue kit. 400, 50, 300, 100, and 50 ng of DNA for E. coli strains UMB1180, UMB1162, UMB1225, UMB0103, and UMB1220 was combined, respectively. Additionally 100, 120, and 54 ng of E. faecalis, P. mirabilis, and S. epidermidis DNA was added, respectively.  The mixed DNA sample was normalized to 500 pg/microliter in elution buffer. The DNA was amplified using the following primers: AGRGTTYGATYMTGGCTCAG (27F) and RGYTACCTTGTTACGACTT (1492R). A SMRTbell library was constructed from the PCR products.