title: GSM8074495: S1_Saline; Mus musculus; RNA-Seq
library_construction_protocol: Spleens were taken on day +7 after BMT and pooled from every 2 mice within the same group (n = 6 mice per group) leading to 3 samples per group. The samples were then stained with CD4-PE, CD8-FITC, CD90.2-Pacific Blue, 7-AAD and Hashtag (CD4, CD8, CD45.1 and Total Seq C)  and subsequently sort-puried to CD4+ and CD8+ T cells. The sort-purified CD4 and CD8 T cells were combined at 1:1 ratio within the same group leading to 3 specimens for 10X capture. Samples were resuspended at 1000 cells/uL and libraries  were  generated  using  the Chromium Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) following the manufacturer's protocol (CG000331, Pleasanton, CA). Final library quality was assessed using the Tapestation 4200 (Agilent, Santa Clara, CA) and libraries were quantified by Qubit dsDNA kit .Pooled libraries were then subjected to paired-end Illumina sequencing according  to the  manufacturer's  protocol