title: GSM6585710: Primary colorectal cancer RNA-Seq, patient #270; Homo sapiens; RNA-Seq
library_construction_protocol: Tumour core samples were dissected from surgical specimens and immediately frozen in liquid nitrogen and initially stored at -80°C. RNA was extracted from <20 mg of tissue using RNEasy Plus Mini Kit (Qiagen, Hilden, Germany), including DNAse treatment, following tissue disruption using a Retsch Mixer Mill. RNA-sequencing was performed using the Illumina HiSeq 2500 V4 platform (Illumina, San Diego, CA, USA) to produce 125bp paired end reads.    Library preparation, including ribosomal RNA depletion using RiboZero Gold, was carried out using Illumina TruSeq V2 reagents, and libraries were sequenced using an Illumina HiSeq 2500 instrument.