title: GSM8164526: MLN_WT; Mus musculus; RNA-Seq
library_construction_protocol: Tissue samples were digested with 1 mg/ml collagenase IV for 20 minutes. Cells were filtered with a 70-μm cell sieve and stained with Live/Dead fixable exclusion dye, followed by fluorochrome-conjugated antibodies in PBS with 2% fetal bovine serum (FBS) and 1mM EDTA. Live/CD45+/CD3+/CD4+/YFP+ cells were sorted. Cells were loaded for capture using the Chromium System with single-cell 3' reagent kit v2 from 10X Genomics. After capture and lysis, cDNA synthesis and amplification were performed according to manufacturer protocol. Amplified cDNA was used to construct the Illumina sequencing library and sequenced on the NovaSeq 6000.