title: GSM6974539: Liver, Tet2KO transplanted, rep9; Mus musculus; RNA-Seq
library_construction_protocol: Animals were sacrificed at 11 weeks of diet and livers were perfused with PBS and harvested. RNA was isolated using Rneasy mini plus kit (Qiagen). 1.0-1.3 ug of total RNA was used for the construction of sequencing libraries. cDNA libraries for RNA-seq were prepared using NEBNext Ultra II RNA Library Prep Kit following manufacturer's protocols. Pooled libraries were sequenced using Illumina NovaSeq 6000.