title: 16S of cultivated biocrust: field cultivation, Colorado Plateau inoculum, time 2, no substrate, water regime A, sample 201
design: DNA was extracted from soil samples using the QIAGEN Power Soil kit. We PCR amplified the 16S gene using 515f/806r primers with Illumina barcodes. We confirmed amplification with gel electrophoresis and then cleaned up the library with the SequalPrep Normalization kit.