title: GSM8296573: WT3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from ~25 mg of frozen left ventricular tissue using RNA-STAT (Tel-Test, Inc) and cleaned-up using the PureLink RNA Mini Kit (Invitrogen). RNA was then pooled between 2 different fl/fl and CS-Mpc2-/- hearts, resulting in n=3 samples per group. RNA concentration and quality were determined by 2100 Bioanalyzer RNA 6000 Nano Kit (Agilent) and depleted of rRNA using RiboMinus Eukaryote Kit v2 (Ambion). DNA libraries were constructed using Ion Total RNA-seq Kit v2 (Thermo Fisher Scientific) and sequenced on an Ion Torrent Proton DNA sequencer (Thermo Fisher Scientific).