title: GSM6925286: GBM, Recurrent, Patient 47; Homo sapiens; RNA-Seq
library_construction_protocol: Library preparation was performed with 1-50 ng of total RNA cDNA was prepared using the SeqPlex RNA Amplification Kit (Sigma-Aldrich, St. Louis, MO, USA) per manufacturer's protocol, and Illumina sequencing adapters were ligated to the ends of cDNA fragments. These were amplified for 12 cycles using primers incorporating unique index tags.