title: GSM7246679: Day 5 infection Tfh-1; Mus musculus; RNA-Seq
library_construction_protocol: For infected recipient mice, on day 5 (not peak response, but the Tfh and non-Tfh cell fate are committed), isolate Tfh and non-Tfh cells from the spleen by cell FACS sorting. RNA was harvested using the NucleoSpin RNA Columns of MACHEREY-NAGEL. 1.5-2.0 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.