title: GSM6721513: HEKa + Activated + Treatment with Rapamycin 2 [HEKT17_RAP2_1]; Homo sapiens; RNA-Seq
library_construction_protocol: All groups were harvested in trizol and immidiately transferred to -80℃ fridge. A magnetic bead system was used to separate messenger RNA from total RNA.  Second strand cDNA was synthesized using either dUTP (for directional library) or dTTP (for non-directional library) after fragmentation. This library was ready for amplification and purification following end repair and A-tailing. We had the directional library ready for amplification and purification after end repair and A-tailing. The library was quantified using Qubit, and the size distribution was detected with a bioanalyzer. Amount of data and effective library concentration will be pooled and sequenced on Illumina platforms.  The index-coded samples were clustered as directed by the manufacturer. These were then sequenced on an Illumina platform to obtain paired-end reads.