title: RNA-Seq of sediments: vent 3
design: Total DNA extraction was performed using MoBio PowerSoilTM following the manufacturer's protocol from approximately 1g aliquots from every vent and background sediment sample, ~0.5 g of 4 out of 5 mat samples (M1, M2, M3, M4) and duplicate 250 mL background water samples (BW1 and BW2) filtered through 0.2 m polycarbonate Whatman Nucleopore Track-Etched Membranes. Prokaryotic communities were analyzed by sequencing the v3  v4 hypervariable region of the 16S rRNA gene. Sequencing libraries were generated using the primer pair S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 and the Herculase II Fusion DNA Plymerase Nextera XT Index Kit V2 kit. Libraries were sequenced on the Illumina MiSeq platform (Macrogen, Korea) to generate 300bp paired end reads.