title: MinION sequencing
library_construction_protocol: Libraries for PCR-cDNA sequencing were prepared following the instructions in the PCR-cDNA sequencing protocol (SEQK-PCB109) from Oxford Nanopore Technologies with minor modifications. Briefly, the VN Primer was replaced with a custom 3� cDNA RT primer (5_- ACTTGCCTGTCGCTCTATCTTCATTGATGGTGCCTACAG-3_, 2 �M).