title: RNA-Seq of poplar: stems of Expansin OE trangenic plants
design: The mRNA was enriched with magnetic beads containing Oligo (dT), and randomly interrupt mRNA by adding Fragmentation Buffer. The  first cDNA and second strand were synthesized Using mRNA as a template, and  the purified double stranded cDNA is subjected to end repair, A-tail addition and sequencing adapters connection, followed by fragment size selection using AMPure XP beads. Finally, a cDNA library was obtained through PCR enrichment.