title: 5'-P RNA-seq of M. abscessus: VapC5-Expressing 6 h
design: 5' P libraries were constructed as previously described (Schifano et al., 2014 Nat. Comm.). Briefly, adaptors were ligated with T4 RNA ligase 1, followed by Reverse transcription with Superscript IV, and PCR with adaptor-specific primers.