title: GSM8411066: T0_Rep1; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted and fragmented in 0.2 M NaOH for 10 minutes on ice. Biotinylated nascent RNAs were then purified on M-280 streptavidin beads (Invitrogen). Enzymatic steps were conducted with RppH (NEB) and PNK (NEB) to remove the 5' cap, repair the triphosphate, and repair the 5' hydroxyl ends. Adapter ligation and reverse transcription were performed with SuperScript III (Invitrogen), followed by PCR amplification. Libraries were size-selected with AMpure XP (Beckman Coulter)