title: GSM8036729: 3_BAY2506856_100nM_1h; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNAeasy Kit (Qiagen, #74181), and cDNA was prepared using the LunaScript® RT SuperMix Kit (New England Biolabs, #E3010) according to manufacturer's instructions. TruSeq Stranded mRNA Library Prep (Illumina, RS-122-2103) was used to construct cDNA libraries starting with an input of 400 ng of total RNA per sample. cDNA libraries were pooled and clustered to a single-read sequencing flow cell (Illumina cBOT, HiSeq SR Cluster Kit v4, GD-401-4001 and sequenced on an Illumina HiSeq 2500 instrument (HiSeq SBS Kit v4 (50 Cycle Kit), FC-401-4002).