title: GSM7256977: CD28 CAR, day 14, scRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: CAR T cells from chronic stimulation cultures were isolated using flow-based sorting. Cells were processed using the 10x Genomics Chromium Single Cell V(D)J Reagent Kits (10x Genomics, PN-1000006, PN-1000020, PN-120262) to generate single-cell emulsions for barcoding, reverse transcription and cDNA amplification. Immediately following these steps, 10x barcoded fragments were pooled and attached to standard Illumina adaptors to generate a barcoded single-cell RNA library.