title: bacterial metataxonomics 16S rRNA from fermented vegetable;  mixed fermentation of Beetroot/red onion collected the day 22 Mar 2021; sample code (VS-T30-B)
design: The DNA was extracted using Master Pure complete DNA and RNA purification kit (Epicentre, Madison, WI, USA). DNA quality and concentration was evaluated with NanoDrop spectrophotometer (Thermo Scientific, Milan, Italy). Library of V3-V4 region were constructed from the 16S rRNA gene region of bacterial DNA.PCR products were purified by means of an Agencourt AMPure kit (Beckman Coulter, Milan, Italy), and the resulting products were tagged with sequencing adapters using the Nextera XT library preparation kit (Illumina Inc, San Diego, CA). Sequencing was performed using a MiSeq Illumina instrument (Illumina) with V3 chemistry, which generated 2X250 bp paired-end reads. MiSeq Control Software, V2.3.0.3, RTA, v1.18.42.0, and CASAVA, v1.8.2, were used for the base-calling and Illumina barcode demultiplexing processes.