title: GSM8296525: PBS_scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Single cells were obtained by cell sorting, staining for lineage markers (Lin), cKit/CD117, and Annexin V.  Prior to sorting, DAPI was added for viability.  Cells stained as Lin-cKit+Annexin V-DAPI- were sorted, pooled from each group, and added to the 10X chip. Single cell library was prepared using “Chromium Next GEM Chip G Single Cell Kit”, 16 rxns PN-1000127 and “Chromium Next GEM Single Cell 3ʹ Kit v3.1”, 4 rxns PN-1000269 and “Dual Index Kit TT Set A”, 96 rxns PN-1000215, following “Chromium Next GEM Single Cell 3' Reagent Kits v3.1(Dual Index) User Guide” (Document number CG000315). single-cell 3' mRNA Transcriptomics