title: Single-cell genomic sequencing of multiple displacement amplification products from aerobic anoxygenic phototrophic bacteria cells by Raman-based single-cell sorting
design: total amount of 1g DNA per sample was used as input material for DNA sequencing preparations. Sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) following manufacturers recommendations and index codes were added to attribute sequences to each sample. Briefly, the DNA sample was fragmented by sonication to a size of 350 bp, then DNA fragments were end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing with further PCR amplification.