title: GSM8262202: Mouse Kidney, Mdm2 Ex 5/6, Untreated Control, Rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Whole tissue RNA was extracted using the Qiagen RNeasy kit (Qiagen, 74104) RNA was tested on an Agilent 2200 TapeStation (D1000 screentape) using RNA screentape and only samples with a RIN>7 were further processed for library preparation. 20ng/ml of RNA were used to prepare libraries using the TruSeq Stranded mRNA Kit. Agilent 2200 Tapestation was used to assess library quality and Qubit (Thermo Fisher Scientific) was used to check concentration. The libraries were then run on the Illumina NextSeq 500 using the High Output 75 cycles kit (paired end, 2x36 bp cycle, dual index (I5 and I7 Illumina)). TruSeq Stranded mRNA Kit