title: GSM6721370: CTRL1101_iMeLC_bulkRNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: TET1 CTRL and CDKO hESCs were differentiated as described above into iMeLCs for bulk RNAseq.  Sorted cells or cell pellets were lysed in 350μL of RLT buffer (QIAGEN), and total RNA was extracted with RNeasy micro kit (QIAGEN, 74004). Libraries were constructed with SMARTer Stranded Total RNA Sample Prep Kit (Takara 634861) following the manufacturer's instructions.