title: GSM6503144: DIPG13, H3K27M-KO, IR5Gy, rep1; Homo sapiens; OTHER
library_construction_protocol: cells were lysed in TRIzol and total RNA isolated according to the TRIzol protocol (Invitrogen). Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. These strand-specific libraries were made using a universal ligation adapter and dual-index, barcoded primers for PCR. Sequencing was performed on a NovaSeq 6000 (Illumina), yielding 150 bp, paired-ended reads. RNA-seq (Bru-seq)