title: GSM6585509: RNA-seq DE 24h biol rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq:  Total RNA was extracted using Quick-RNA MiniPrep kits (ZYMO research; R1055).  cDNA was produced by using High Capacity cDNA Reverse Transcription Kit (AppliedBiosystems; 4368817) RNA-seq: 500ng of total RNA underwent polyA selection and TruSeq library preparation following Illumina (TruSeq Stranded mRNA LT Kit; RS-122-2102), with 8 cycles of PCR. Samples were barcoded and run on a HiSeq 4000 or NovaSeq 6000 platform in a PE50 run, using the HiSeq 3000/4000 SBS Kit or NovaSeq 6000 SP or S2 Reagent Kit (100 Cycles) (Illumina).