title: GSM8073572: RdrpoS_LBagar_1; Vibrio cholerae O1 biovar El Tor; RNA-Seq
library_construction_protocol: TRIzol-Chloroform total RNA extraction was performed as stated in manufacturers instructions (Invitrogen/Fisher), with the exception that when isopropanol was added, an equal volume of High Salt solution (0.8M Sodium Citrate, 1.2M NaCl) was also added to account for high levels of polysaccharide present. Samples were DNAase treated, and RiboZero Plus was used for rRNA depletion. Illumina Stranded RNA library preparation (no modifcations from protocol)