title: GSM8372559: Dlk1+ hepatoblasts, siRab35, replicate3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Mini Kit (Qiagen, Cat. No. 74104 50) including the DNase I treatment step (Qiagen, cat. No. 79254). RNA libraries for RNA-seq were prepared using 300 ng input total RNA, mRNA was enriched by poly-dT pull down with NEBNext Poly(A), libraries were prepared withNEBNext Ultra II Directional RNA Library Prep kit for Illumina following manufacturer's protocols. 75bp single read sequencing on Illumina NextSeq 500.