title: GSM6502654: N2 L3 animals, treated with EV control RNAi from hatch, Replicate 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Worms were collected in TRIzol Reagent (Invitrogen), flash frozen in liquid nitrogen, and stored at -80°C prior to RNA isolation. Samples were lysed using metal beads and the Tissuelyser I system (Qiagen). RNA was isolated from lysate using Direct-zol RNA Miniprep kit (Zymo). Approximately 1 ug of RNA  was submitted to Genewiz (Azenta) for sequencing. All total RNA quality control, mRNA-sequencing library preparation, and Illumina sequencing was performed by Genewiz (Azenta). Samples were first verified for RNA integrity scores greater than 8 using an Agilent Tapestation 4200. Illumina library preparation was performed using polyA selection for mRNA species. Approximately 20 million paired-end, 150 base pair reads were obtained per sample.