title: GSM8018548: RT-17; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen adipose tissue was disrupted using the Qiagen TissueRuptor (VA and PLHIV cohorts) or ceramic beads and grinder system (MP Biomedicals LLC FastPrep-24 5G and Lysing Matrix D tubes with 1.4mm Ceramic beads for the discovery cohort) in Qiazol Lysis Reagent. RNA was purified using the Qiagen RNEasy Plus Mini kit. An on-column DNAse digestion was performed for the discovery and VA cohorts. NEBNext Ultra II RNA Library Prep Kit (NEB) per manufacturer's instructions