title: GSM6757636: ChIPseq-BCL7A-GFP bcl7a-1-bio1; Arabidopsis; ChIP-Seq
library_construction_protocol: In brief, 14-day-old seedlings (0.5 g for each biological replicate) grown on ½-strength MS medium under long-day  conditions were fixed using 1% formaldehyde under a vacuum for 15 min and then ground into a fine powder in liquid nitrogen. The chromatin was sonicated into 300-500 bp fragments using a Bioruptor sonicator with a 30/45-s on/off cycle (27 total on cycles). Immunoprecipitation was performed using 1 μl of anti-GFP (Abcam, Cat. No. ab290) plus 40 μl Agarose beads (Abcam, Cat. No. 16-157) at 4°C overnight. For ChIP-seq, 1 g of seedlings was used, and the ChIPed DNA was purified using the MinElute PCR purification kit (Qiagen, Cat. No. 28004). Libraries were constructed with 2ng of ChIPed DNA using the VAHTS Universal DNA Library Prep Kit for Illumina V3 (Vazyme Biotech, Cat. No. ND607), VAHTSTM DNA Adapters set3-set6 for Illumina (Vazyme Biotech, Cat. No. N805) and VAHTS DNA Clean Beads (Vazyme Biotech, Cat. No. N411-02) according to the manufacturer's protocol. High-throughput sequencing was performed at Novagene (sequencing method: NovaSeq-PE150).