title: GSM7866370: Jurkat cells, unstimulated, replicate 3, RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified from cells with the Maxwell RSC 48 instrument using the Maxwell RSC simplyRNA Cells Kit (AS1340, Promega). RNA-seq library preparation was performed by Novogene using standard protocols. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, PCR amplification, and purification.