title: GSM8036062: Crosslinked, IPSC, control,CHMP7 IN, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)