title: GSM7744912: 3152PBLs-PSPB6; Bos taurus; RNA-Seq
library_construction_protocol: Blood was collected at the end of the treatment schedule, 12 hours after last infusion (8:30 am of day 17 or day 20). Blood was collected from tail vein into K3 EDTA vacuette tubes (Greiner Bio- one) and peripheral blood leukocytes (PBLs) were isolated as described in Kamat et al., (2016). RNA was isolated from PBLs isolate (15 million cells) following homogenization in 800 μL of TRIzol reagent (Life Technologies). Total RNA was purified using RNA Clean and Concentrator kit (Zymo Research) to eliminate contaminating DNA and reagents. RNA samples were shipped on dry ice to BGI laboratory (Beijing Genomics Institute, Shenzhen, China) where RNA quality and quantity were assessed using Agilent 2100 Bioanalyzer (Agilent Technologies). All samples met the quality criteria for RNA sequencing, showing RNA integrity number (RIN) between 8 and 9.9 values. Preparation of mRNA library and sequencing were performed following BGI DNBSEQ technology platform with paired end 100 bp (PE100).