title: GSM8297389: PN0524_0002-Cold-MTG; Homo sapiens; Bisulfite-Seq
library_construction_protocol: For gDNA purification samples were added to the nuclease-free water (Thermo AM9939) to the final volume of 100µl. Then 3µl of RNase A (Thermo R1253) was added to the samples and incubated for 20 minutes at 37oC. Further DNA was purified using Monarch® Genomic DNA Purification Kit (NEB T3010S) according to the manufacturer's instructions with minor modifications. 1µl of Proteinase K (Thermo EO0491) was added to the samples and 100µl of lysis buffer was added and incubated for 5 min at 57oC followed by 400µl of binding buffer was added and loaded to the purification columns. Washed twice with wash buffer and eluted with 15µl of elution buffer. Purified DNA samples were quantified using Qubit high-sensitivity dsDNA Quantification assay (Thermo Q32854). The quality of the gDNA was analysed using Agilent TapeStation systems with high-sensitivity genomic DNA tapes. Library preparation performed with NEBNext Enzymatic Methyl-Seq Kit.