title: GSM8494124: endothelial cells，high glucose; Danio rerio; RNA-Seq
library_construction_protocol: Embryos were transferred into a 15 mL falcon tube with 10 mL phosphate buffered saline (PBS) containing 0.25% trypsin and incubated in a rolling machine for 30 min at 28°C during which they were pipetted up and down per 5 min. After centrifuging for 5 min at 800 g at 4°C, cells were resuspended in PBS containing 0.25% trypsin and 15% fetal calf serum (FCS) to stop the digestion and centrifuged for 5 min at 800 g at 4°C. Cells were rinsed with PBS containing 2% FCS for 2 times and resuspended in PBS. EGFP positive cells were first enriched by fluorescence-activated cell sorting (FACS), and then went through library preparation following the standard protocol named Chromium Single Cell 3'Reagent Kits v3 from 10X Genomics Company.