title: GSM8036426: K562 cells, DualCRISPR pilot screen of putative K562 enhancers, 15 days, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: The single-cell dual-CRISPR library was introduced into K562-Cas9 expressing cells via spin infection at a final MOI of <0.3. After one week, cells that successfully incorporated the single-cell dual-CRISPR system were sorted based on GFP expression using FACS sort. To prepare for single-cell processing, 0.5 × 106 cells at a concentration of 1,500 cells ml-1 in 0.04% BSA-PBS were used for single-cell RNA and guide RNA capture according to the 10x Genomics protocol. The 10x Genomics Chromium platform was used to generate the single-cell libraries according to the manufacturer's protocol, which were sequenced on the Illumina NovaSeq 6000 platform.