title: GSM8164416: HV1 CD4+ and CD8+ T-cells, TCR; Mus musculus; OTHER
library_construction_protocol: A 50:50 mixture of CD45.2+ HV1 CD4 T cells as well as CD44+ CD8 T cells were sorted from the indicated experimental treatment arms. The sorted T cells were labeled with treatment arm-specific hashtag oligonucleotide (HTO) antibodies, as well as CITE-Seq antibody derived oligonucleotide tag (ADT) antibodies to CD4, CD39, CD4b, PD1, TIM3 and LAG3. Cells were captured and single cell sequencing for gene expression (GEX), sample-specific hashtag and protein feature barcodes, and T-cell receptor (TCR) repertoires was performed using the 10X Genomics Chromium Next GEM Single Cell 5' (ver. 1.1) reagents