title: GSM8213675: BAL Cells, Silica Exposed, Complicated Silicosis, Patient 7; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from BALcells using a RNeasy kit (Qiagen, #74106) following the manufacturer's instructions. Illumina's TruSeq stranded RNA sample preparation protocol (Illumina, San Diego, California, USA). This protocol includes the following steps: cDNA Synthesis: (Purification of mRNA (via oligo(dT) beads), Fragmentation of mRNA with divalent cations and heat, 1st strand cDNA synthesis (randomly primed), 2nd strand cDNA synthesis; cDNA library preparation: DNA fragment end repair (blunt ending of DNA fragments), 3' Adenylation of DNA fragments, Sequencing adapter ligation (utilizing T-A pairing of adapter and DNA fragments), Amplification of library via PCR