title: GSM8036874: Bbs8 KO iWAT SVF, p1, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Progenitor cell isolation from the stromal vascular fraction (SVF) was performed as previously described (Sieckmann et al., 2022).Tissues were minced, and digested with collagenase II and DNAse I at 37°C for 30 min. The digestion was quenched by adding AT buffer (0.5% BSA in PBS). The dissociated cells were passed through a 100-μm filter (Corning). The stromal vascular fraction, was resuspended in red blood cell lysis buffer (BioLegend) and further filtered through a 70-μm filter (Corning). Seq-Well S3 protocol