title: GSM7831294: mWake(Cre/+), Lateral amygdala, scRNA, LA3; Mus musculus; RNA-Seq
library_construction_protocol: For scRNA-Seq, tissues were dissociated in papain (Worthington) and debris was removed using OptiPrep density gradient media following cell dissociation. Cells were then processed immediately for scRNA-Seq. scRNA-Seq following 10x V3.1 guideline For snRNA-Seq, tissues were processed following a modified 10x Genomics protocol. Lysis buffer containing (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% Nonidet-P40, 0.01% Digitonin, 1 U/ml Rnase inhibitor, 1% BSA) was added into a tube containing the lateral amygdalae and incubated on ice for 15 min with gentle pestle grinding (5 strokes every 3 min).  Wash buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.2 U/ml RNase inhibitor, 1% BSA) was added, and nuclei were raw through a 50 μm filter. Debris was removed using OptiPrep density gradient media and nuclei morphology was accessed under the light microscope.  Nuclei were then immediately processed for snRNA-Seq.