title: GSM6663241: Digested tumor, Untreated, Day 5; Mus musculus; RNA-Seq
library_construction_protocol: Subcutaneous tumors were dissected, cut into small chunks, and digested with Collagenase IV (Millipore Sigma) for 30 min at 37C, followed by physical disruption through a 100uM cell strainer. Tumor cells were stained with CD45.2, endogenous immune cells were stained with CD45.1, and dead cells were stained with Fixable Live/Dead stain for FACS based purification. Live CD45.1+ immune cells and live CD45.2+ tumor cells were FACS isolated, counted, and pooled at a 1:1 ratio. Library was performed according to the manufacter's instructions (single cell 5' v2 protocol, 10x Genomics)