title: GSM8035774: Exponential cells, assay 3, 30 min; Staphylococcus epidermidis; RNA-Seq
library_construction_protocol: Samples for RNA isolation were taken from the non-infected cultures shortly before infection (0 min) and from the infected cultures at 5, 15, and 30 min post-infection. Samples were immediately mixed with a 1:10 volume of an ice-cold stop solution. Total RNA extraction was performed using the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions with some modifications. Genomic DNA was degraded using the TURBO DNA-free™ Kit (Thermo Scientific). RNA integrity was determined using the Bioanalyzer 2100 equipment and an RNA 6000 Pico Kit (Agilent Technologies). Samples from three independent experiments with an RNA Integrity Number (RIN) above 8 were selected for RNA-seq. RNA samples were sent to Novogene (UK) for library preparation and prokaryotic RNA sequencing using the Illumina NovaSeq 6000 Sequencing System (paired-end 150 bp).