title: GSM6722022: Hdac1 CUT&RUN in naïve CD4 cells [nai.hdac1.1]; Mus musculus; OTHER
library_construction_protocol: Sorted naive CD4+ T or Tfh cells were crossed-linked with formaldehyde, and then incubated with Hdac1 antibody overnight, followed by incubation with Protein A/G-MNase, calcium-mediated MNase activation. The reaction was stopped and then genomic DNA fragments were extracted. The DNA segments from Hdac1 Cut&Run were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-X platform.