title: GSM6721856: T24 cells, sgNC_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total amounts and integrity of RNA were assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) Sequencing libraries were constructed by NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Qubit2.0 Fluorometer, Agilent 2100 bioanalyzer and Quantitative real-time PCR (Q-PCR) were carried out successively to ensure the quality of the library.