title: GSM7359858: A375_DMSO_R1 [DMSO_245]; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed twice with ice cold PBS, then scrapped with 1ml of PBS. Then they were centrifuged (500g for 1min at 4°C) and slowly resuspended in 1ml of lysis buffer (10mM Tris-HCL pH7.5; 5mM MgCl2; 100 mM KCl; 1% Triton X-100), and incubated on ice for 10 minutes. After this, lysates were centrifuged (1000g for 10minutes at 4°C), and RNAs were extracted from cytoplasmic fractions using tri-reagent (Sigma, T9424) following the manufacturer's instructions. RNA samples were sent for sequencing at Novogene (rRNA depletion library preparations, sequencing on Novaseq 6000 2x150)