title: GSM6873876: gan1162; Mus musculus; RNA-Seq
library_construction_protocol: Freshly collected liver cells from mice were washed with DPBS, diced and then extracted using Liver Dissociation Kit (mouse) with gentleMACS Dissociator from Miltenyi Biotec according to the manufacturer's instructions. Then the resuspended samples were sequentially applied to 70μm and 30μm strainer. The red blood cells were lysed with Red Blood Cell Lysis Solution. Then, after washing, the isolated liver cells were centrifuged with 50g for 30 seconds to remove most of hepatocytes. Next, cells in supernatant were washed and re-suspended in DPBS. A small portion of the cells were used for checking viability by staining with a Viability Dye. Finally, cells were washed in DPBS, centrifuged and resuspended in RPMI1640 medium with 5% BSA. Library was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x Genomics).