title: GSM8018692: Kaposi's sarcoma-derived cell line, size matched input 1; Homo sapiens; RIP-Seq
library_construction_protocol: 48 h hours post induction the cells were washed and crosslinking with 150 mJ/cm2 of UV light at 254 nm . Cell were lysed and sonicated with Diagenode Biorupter Sonication System with a low setting, at 4°C for 5 min 30 sec on/30 sec off. RNA was cleaved by RNase I for 3 minutes at 37°C. 3' RNA adapter were ligated to the RNA. RT performed by SuperScript III using a primer for the RNA adapter. Second adapter were ligated to the 3' end of the cDNA. Library amplify by PCR using Q5 polymerase. Library were size selected by 3% low melt agarose gel