title: GSM8296360: R. Hippocampus, C57BL6 mouse, Cre-positive EZH2 wildtype, 4 days post-saline, rep3; Mus musculus; RNA-Seq
library_construction_protocol: On day 4 post-SE, hippocampi were extracted, flash frozen in liquid nitrogen, and stored at -80 degrees Celsius until all samples were accrued. RNA was harvested with Trizol, and clean-up was carried out with the RNeasy Mini Kit (Qiagen). Purified samples were submitted to the Gene Expression core at the University of Wisconsin-Madison Biotechnology Center for library preparation, quality control analysis, and Illumina sequencing (https://biotech.wisc.edu). RNA libraries were prepared via H/M/R (Gold) cytoplasmic and mitochondiral rRNA depletion.