title: GSM6757655: AHH-1 cells, control, 0 Gy [AHH102]; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA of samples for radiation differential expression circular RNA screening was isolated by TRIzol reagent (Ambion, 15596026) at post-irradiation 4 h. he libraries were constructed via Total RNA-seq (H/M/R) Library Prep Kit for Illumina (Vazyme，China). The size of the libraries was detected by Agilent 2100 Bioanalyzer. And the library's effective concentrations were accurately determined by Q-PCR (effective concentrations > 3 nM).