title: Caki1 Kaiso KO rep4
design: otal RNA was extracted from cells with TRIzol reagent according to the manufacturers instructions (Thermo Fisher Scientific) in triplicate. Quality was checked with BioAnalyser and RNA 6000 Nano Kit (Agilent Technologies, Santa Clara, CA, USA). PolyA RNA was purified with Dynabeads mRNA Purification Kit (Ambion, Inc., Austin, TX, USA). An Illumina library was made from polyA RNA with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manual