title: GSM8259871: p2 utricle, gentamycin+BMP4, CUT&Tag; Mus musculus; OTHER
library_construction_protocol: Accutase was used for cell detachment and tissue dissociation of the utricle explants for 15–20 min at 37°C with gentle trituration every 5 min (50 times with a 200 µl low‐retention pipette). Lysates containing a total of 10,000 cells were obatined Lysates containing a total of 10,000 cells were incubated at room temperature with concanavalin A-coated magnetic beads for 10 minutes and then with the primary antibody against c-Fos (1:50 dilution; ab25949, Abcam) or IgG control antibody (normal rabbit IgG: Millipore cat. No. 12-370) overnight at 4°C, with secondary antibodies (1:100 dilution; anti-rabbit IgG antibody, goat monoclonal: Millipore AP132) for 2 h, and then with pA/G-Tn5 adapter complex for 1 h. DNAs were extracted using a DNA purification kit containing selection beads (Yeasen, 12601ES03), and a fixed amount of E. coli DNA was added for spike-in calibration. Libraries were prepared using the Hieff NGS Tagment Index Kit for Illumina (96 index) (Yeasen, 12610ES96), and the size distribution of the libraries was determined using an Agilent 4200 TapeStation. Paired-end 150 bp sequencing was carried out on a NovaSeq 6000 platform following the manufacturer's instructions.