title: GSM7350610: 6hpa_control_H3K9me3_rep2; Mus musculus; OTHER
library_construction_protocol: 50 blastomeres were used per reaction. Each sample were bound with 5 μL concanavalin-coated magnetic beads and incubated with 1 μg antibody at 4℃ for 2 hours. Deluted pA-MNase was incubated with samples at 4℃ for 1 hour and chromatin cleavage was initiated with 100mM CaCl2,for 15 minutes at 0℃. RNA and proteins were then digested. Fragmented DNA was purified by phenol chloroform followed by ethanol purification. Libraries were generated using the KAPA HyperPrep Kit for the Illumina platform (kk8504), following the manufacturer's instrunctions. Paired-end 150 bp sequencing was performed in NovaSeq (Illumina) platform in Berry Genomics and Novogen.