title: Piglet ileon digestate sample, block 6 ,5%BC treatment, day 26, high weigth, animal R003
design: DNA extraction was performed using the QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturers instruction. The DNA was extracted from digestates using a bead beating method (Zaheer et al., 2018) with minor modifications: frozen fecal sample was diluted with 0.9% saline (1:2) before processing, and the homogenization step was done in a Bead Ruptor at 4,000 rpm during 3 minutes. For all samples, the DNA integrity was verified by electrophoresis in 1% agarose gel. and quantified using the NanoDrop apparatus and the Quant-iT PicoGreen kit (Invitrogen)