title: vernalization 42days
design: The library was constructed using the NEBNext UltraTM RNA Library Prep Kit (NEB, USA), with a slight modification in the process. VAHTS mRNA Capture beans (Vazyme Biotech, Nanjing China) were used for the isolation of mRNA, and VAHTS DNA clean beans (Vazyme Biotech, Nanjing, China) were used for the purification of double-stranded cDNA and PCR products, and the selection of fragments of connecting products. The constructed cDNA library of wheat leaves subjected to different vernalization treatments was then sequenced using the Illumina HiSeq 4036 sequencing system (Illumina, USA).