title: Sediment Bacteria
design: For Bacteria and Eukaryota, primers were used for PCR amplification of SSU rRNA gene fragments as described in The Earth Microbiome Project (Caporaso et al., 2011; Stoeck et al., 2010). For Archaea, the primer pair used was published in Takahashi et al. (2014). The primers contained 5 common sequence tags (known as common sequences 1 and 2, CS1 and CS2 (Naqib et al., 2018). Amplicons were generated using a two-stage PCR amplification protocol.