title: RNA-Seq of cricetulus griseus: low temperature/pH
design: Total RNA extraction was performed with the RNeasy Plus Mini kit (Qiagen) according to  manufacturers protocol. Quality and integrity were validated on a NanoDrop 8000 UV-Vis spectrophotometer (Thermo Scientific, MA, USA) and Agilent 2100 Bioanalyzer System (Agilent, Santa Clara, CA) with RNA 6000 Nano kit (Agilent), respectively. All samples had RNA integrity numbers (RIN) values >9. RNA samples were normalized to a standard input concentration (1 g of total RNA) and an Illumina compatible sequencing library was prepared with the TruSeq RNA sample preparation v2.0 kit (Illumina, San Diego, CA) following the manufacturers recommended procedures. The resulting cDNA libraries were quality validated on an Agilent 2100 Bioanalyzer System to ensure proper fragment distribution (~260 bp) and effective removal of adapter dimers. Sequence libraries were multiplexed and sequence data collected on 2 lanes of a HiSeq 2500 (Illumina, San Diego, CA) at the Genomics division of David H. Murdock Research Institute (Kannapolis, NC) using a 2101 bp, paired end read type. Raw sequence reads were demultiplexed on the instrument .