title: GSM8213677: SPF-A, feces; Sus scrofa; RNA-Seq
library_construction_protocol: DNA was extracted from the stool samples and 16S amplicon libraries were constructed using Herculase Ⅱ Fusion DNA Polymerase Nextera XT Index Kit V2 (Illumina, San Diego, CA, USA) through the services provided by GnC Bio Co. (Daejeon, Korea). Sequences of 16S rRNA at V3 and V4 region were targeted to amplify. The quality and quantity of amplicon libraries were assessed using D1000 ScreenTape (Agilent, Palo Alto, CA, USA) and Picogreen (Agilent), respectively.