title: RNA-seq of untreated H460 cells
design: For RNA extraction, the RNA Solo Kit (Evrogen, Russia) was used with DNase I treatment step in accordance with manufactures recommendations. RNA quality and concentration were evaluated on 2100 Bioanalyzer (Agilent Technologies, USA) by Ag-ilent RNA 6000 Nano Kit (Agilent Technologies, USA) and Qubit 2.0 (Life Technologies, USA) with Qubit RNA BR Assay Kit (Life Technologies, USA) respectively. For cDNA library preparation, 1 g of total RNA, the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Hitchin, United Kingdom) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) were used. cDNA library quality and concentration were evaluated on 2100 Bioanalyzer (Agilent Technologies, USA) with Agilent DNA 1000 Kit (Agilent Technologies, USA) and Qubit 2.0 (Life Technologies, USA) with Qubit dsDNA HS Assay Kit (Life Tech-nologies, USA) respectively. Transcriptome sequencing was performed on NextSeq 500 (Illumina, USA) with a read length of 86 bp.