title: GSM7794701: Dm, adult, Ctrl, rep1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was harvested using TRI Reagent (Sigma-Aldrich). 1 ug of total RNA was used for the construction of sequencing libraries. ChIP DNA was pulled down by H3K9ac antibody in HEK293T cells. DNA for CUT&Tag-seq was cleavaged Under Target & Tagmentation by RNPC3 or HDAC3 antibody in HEK293T cells. RNA libraries for RNA-seq were prepared using Fast RNA-seq Lib Prep Kit V2 following manufacturer's protocols. DNA libraries for CUT&Tag-seq were prepared using CUT&Tag Assay Kit (pAG-Tn5) following manufacturer's protocols. DNA libraries for ChIP-seq were prepared using Scale ssDNA-seq Lib Prep Kit following manufacturer's protocols.