title: GSM7256796: E17.5_ChIP_input-for-H3K4me3-H3K27me3-CTCF_wildtype_rep1; Mus musculus; ChIP-Seq
library_construction_protocol: Crosslinked diencephalon tissue from E11.5 or E17.5 was used for nuclei isolation, sonication, and chromatin extraction. Each replicate consists of tissue from 3 (at E17.5) or 6 (at E11.5) pooled embryos from the same litter. Chromatin immunopecipitation was performed with H3K27ac (Active Motif #39133), H3K4me3 (Cell Signaling #9751S), H3K27me3 (Cell Signaling #9733S), or CTCF (Diagenode #C15410210-50) antibody-coated magnetic beads (Protein G Dynabeads, Invitrogen) as described in doi:10.1101/pdb.prot084848. Libraries were prepared and sequenced (2×150 bp) using standard Illumina protocols on an Illumina NovaSeq 6000.