title: Nicrophorus tomentosus oral secretion
design: Isolated DNA was  used as a template to PCR amplify the 16S rRNA V4 hypervariable region using the 515F and 806R prokaryotic-specific primer pair modified to include the Illumina overhang adaptors. PCR products were cleaned using PureLink PCR cleanup kit (Life Technologies, Carlsbad, California), and the clean product was used in a second PCR reaction to attach the dual indices and Illumina sequencing adapters using Nexterra XT index kit v2 (Illumina Inc., San Diego, California). These second PCR products were then cleaned using PureLink gel extraction kit (Life Technologies, Carlsbad, California), individually quantified, and pooled using the Illumina library pooling calculator to prepare a 100 pM library that was then sequenced using the paired-end Illumina iSeq-100 sequencing system.