title: GSM8291524: 96hrs, scRNASEQ; Homo sapiens; RNA-Seq
library_construction_protocol: hTLSs were collected from 96WPs and pooled together. TypLE was added to the samples and incubated at 37°C for 15-20 minutes. Samples were then gently pipetted up and down in TrypLE until dissociated into single cells. Cells were then spun in the centrifuge at 170g for 4 minutes, resuspended in PBS-/- + 0.04% BSA, and kept on ice until further processing. SingleCell 3' v3