title: GSM8371996: Hues9; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol (Invitrogen), and reverse transcription was performed with 1 μg of RNA using PrimeScriptTM RT Master Mix (TaKaRa). For RNA-seq analysis, total RNA was extracted using a mirVana miRNA Isolation Kit (Ambion). Samples with an RNA integrity number (RIN) >7, as measured using an Agilent 2100 Bioanalyzer (Agilent Technologies), were subjected to sequencing analysis. cDNA libraries were constructed using a TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequenced with the Illumina NovaSeq 6000 system