title: GSM6757490: Control_reoxy, replicate 4, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Mice lungs were digested with  0.4 mg/ml Liberase and 10 mg/ml elastase. Each sample was dissociated using the gentle MACS Dissociator system following the manufacturer's protocol qnd incubate 37°C for 30 min. The cell suspension was placed on ice, neutralized with cold PBS with 5% BSA, and filtered through a 70 μm cell strainer. The cell suspension was depleted of CD45-positive cells and enriched for EC using CD31 microbeads according to the manufacturer's instructions, and collected in cold PBS with 2% BSA.  Stanford Genome Sequencing Service Center for 10X chromium single-cell RNA-seq paired end library preparation. Library was performed according to the manufacter's instructions (single cell 3' v3.1 protocol, 10X Genomics).