title: GSM6585400: T14_plus_P3; Nodularia spumigena CCY9414; RNA-Seq
library_construction_protocol: Cell cultures were preserved with a 5% phenol(v/v in ethanol) stop solution for 20 min prior to cell harvesting via centrifugation. Cell pellets were suspended in 1 ml TRIzol reagent, followed by bead-beating, incubation at 65°C for 10 min, and RNA extraction according to Steunou et al. (2006). RNA extracts were DNase I digested for 30 min and purified with phenol/chloroform followed by sodium acetate precipitation. mRNA non-stranded library preparation with rRNA depletion performed by LGC, Biosearch Technologies, Berlin, Germany