title: GSM7256696: Nr3c1-Foxp3Cre_tumorTreg_1; Mus musculus; RNA-Seq
library_construction_protocol: Solid tumors were mechanically isolated, digested with Liberase TL and DNase I, tumor cells depleted on a Percoll gradient, and infiltrating cells washed and stained with Blue Live/Dead dye and antibodies against CD45, CD4, CD25, and sorted on a BD FACSAria Fusion. Total RNA was extracted using Qiagen RNeasy mini kits. The SMARTer Ultra Low Input RNA Kit was used to generate cDNA, which was then made into a sequencing ready library using the Nextera® XT DNA Sample Preparation Kit from Illumina. The final purified sequencing ready library was quantitated by qPCR before cluster generation and sequencing on Illumina NextSeq 2000 P2 run.