title: GSM7744868: GST scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Thymic single cell suspensions were obtained by mechanical digestion in the presence of DNase I (100 µg/ml) and Liberase TM (50 µg/ml) for 40 min and washed PBS 5%BSA 1mM EDTA. Samples were enriched for CD45- cells using the autoMACS® Pro Separator with the Depletes program (Miltenyi). Viable CD31+ EC and EpCAM+ TEC were sorted using a BD FACSAriaIII cell sorter. Sorted EC and TEC from each sample were pooled and loaded in a single capture well for subsequent 10x Genomics Single Cell 5' v32 workflow, targeting a capture of 10,000 cells. Libraries were performed according to the manufacter's instructions (single cell 5' v2 protocol, 10x Genomics).  The resulting libraries containing distinct i5 and i7 indexes were pooled and sequenced together on an Illumina NextSeq2000 platform using a P2 flow cell (100 cycles).