title: GSM6721852: total RNA polyA purified rep 1; Escherichia coli str. K-12 substr. MG1655; RNA-Seq
library_construction_protocol: Cell lysis was done using a FastPrep24 homogenizer for 20 sec at 4.0 M/s and RNA and was extracted using hot phenol/choloform Performed at Vertis AG using in-house protocols: Depletion of rRNA molecules, Preparation of strand specific cDNA for bacterial whole  transcriptome sequencing, Removal of long poly(A) tails with CRISPR-Cas9