title: GSM8213719: dpi1_Young_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from homogenized lung tissue using TRizol with standard protocol For polyA based mRNA-seq, 1 μg total RNAs were used for library construction. mRNA was enriched and purified using Library Preparation VAHTSTM mRNA Capture Beads (Vazyme, NR401-01). Next, purified mRNAs were fragmented, followed by cDNA synthesis, second-strand synthesis, end repair, adaptor ligation and amplification using VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, NR605-01) and VAHTS DNA Clean Beads. Libraries were sequenced for on average 20 million pair-end reads.