title: GSM8209954: OCI-AML3, gTSR3, Replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy mini kit (Cat# 74104, QIAGEN) with DNase I treatment (Cat# 79254, QIAGEN). RNA quality was assessed with TapeStation RNA ScreenTape & Reagents (Agilent). Library preparation was performed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (Cat # E7760, New England Biolabs) and the NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) (Cat# E6440, E6442 New England Biolabs) in conjunction with NEBNext® Poly(A) mRNA Magnetic Isolation Module (Cat# E7490, New England Biolabs), with a starting input of 500 ng RNA.