title: RNA-seq of PBI121 after transiently overexpressed in Nicotiana benthamiana for 2 d
design: PBI121 transiently overexpressed in Nicotiana benthamiana for 2 d. Leaves were ground into fine powder under the protection of liquid nitrogen. Total RNAs were extracted using the TRIzol (thermofisher, 15596018). A total amount of 1 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using illumina NovaseqTM 6000 (LC Bio Technology CO.,Ltd. Hangzhou, China) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.