title: GSM7246729: NPC, WT, HiC, biol rep1; Homo sapiens; Hi-C
library_construction_protocol: In situ HiC was performed on wildtype and INTS10 heterzygote NPCs (HET2)  using the Arima HiC+ kit (Arima Genomics). Briefly, according to the standard input protocol, 5 million cells were crosslinked then flash-frozen in aliquots of 1 million cells. After quantifying the DNA from 1 million cells, we used two aliquots of cells per condition to meet the amount of DNA (500ng-5ug) required to proceed with the HiC reaction. Digestion and proximity ligation steps were done according to the kit protocol. Proximally ligated DNA underwent quality control checks for ligation efficiency then they were sonicated with the Covaris ME220 sonicator and size selected with DNA purification beads (AMPure XP Beads) to obtain a fragment size distribution of 200-600bp prior to the biotin enrichment step. Finally, libraries were prepared by end repair, dA-tailing, and adaptor ligation using the NEB Next Ultra II DNA Library Prep kit for Illumina, following a protocol tailored for Arima HiC DNA. NEB Next Ultra II DNA Library Prep kit for Illumina, following a protocol tailored for Arima HiC DNA.