title: GSM6670968: miRNA-Seq Col-0, 21 C, rep3; Arabidopsis thaliana; miRNA-Seq
library_construction_protocol: Seedlings (50-100 mg) were collected by flash freezing in liquid nitrogen and stored at −80 °C until processing. Samples were ground to a fine powder in liquid nitrogen, and RNA was extracted using a Quick- RNA MiniPrep kit with on-column DNase I digestion (Zymo Research, Irvine, CA). 10 µg total RNA from 4-d-old seedlings grown under either 21oC or 27oC was resolved in a 15% urea-PAGE gel, and the 15-40-nt region was excised, and small RNAs were purified from the gel. Small RNA libraries were constructed using the NEBNext Multiplex Small-RNA Library Prep Set for Illumina (E7300).