title: Amplicon seq using universal eukaryotic 18S primers from Black-capped petrel fecal sample
design: We extracted DNA from fecal samples using the Qiagen QIAamp Fast DNA Stool kit. We used the universal eukaryotic primers developed by McInnes et al. (2017a) to amplify the v7 region of the small subunit rDNA (hereafter 18S), allowing us to theoretically identify all prey to the family- or order-level. We visualized PCR products using gel electrophoresis before being diluted and sent for adapter ligation and sequencing at the Hubbard Center for Genome Studies at the University of New Hampshire.