title: GSM7528333: WT hippocampus snRNA-seq 2; Mus musculus; OTHER
library_construction_protocol: Mice were sacrificed and the hippocampus of each mouse was dissected and placed in a lysis buffer (10mM Tris pH 7.4, 10mM NaCl, 3mM MgCl2, 0.1% NP-40 and 1:1000 RNasIN),  according to 10x genomics Nuclei isolation for single cell sequencing protocol. The pellet containing the nuclei was resuspended in a wash buffer (PBS, 1%BSA and 1:1000 RNasIN). 1000/ul nuclei from each sample were taken for library preparation using 10x Genomics Chromium Single Cell Kit Version 3.1 according to manufacturer protocol. All libraries were pooled and sequenced on NovaSeq 6000 to an average depth of approximately 200,000,000 reads per sample.