title: GSM7648540: NB, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Using collagenase D and DNase1, the lung biopsies were minced and digested to isolate individual lung cells, following the protocol described. Dead cells were excluded using the DeadCell Removal kit (Miltenyi Biotic,130-090-101), as per the manufacturer's instructions. Subsequently, live cells were enumerated with a hemocytometer, resuspended in 2% BSA at a density of 3,000 cells/uL, and processed with the 10x Genomics GemCode Single-cell instrument to create single-cell Gel Bead-In-EMlusions (GEMs). Libraries were generated and sequenced from the cDNAs with Chromium Next GEM Single Cell 3' Reagent Kits v 2. cDNA libraries were sequenced on an Illumina Novaseq6000 platform (Illumina).