title: GSM7797159: S2, HS, DMSO, Pol2S5P, rep2; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: samples were were fixed in 1% PFA for 10 min at RT, homogenized using a pestle in 300 ml of RIPA buffer, and sonicated with Bioruptor (Diagenode). Immunoprecipitations were carried out with 5 µg antibody overnight at 4°C in a rotating wheel. ChIP libraries were prepared for Illumina NextSeq 500 using NEBNext ChIP-Seq DNA Sample Prep Master Mix Set for Illumina (NEB E6240) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1) (NEB E7335) according to the manufacturer's protocols.