title: 18S amplicon sequencing Monterey Bay
design: The 18SV4 region was PCR amplified using the primer pair V4F (CCAGCASCYGCGGTAATTCC ) and V4RB (ACTTTCGTTCTTGATYR); one-step PCR using the TruFi DNA Polymerase; purified using AMPure XP beads; quantified and pooled.