title: 16S amplicon sequencing Monterey Bay
design: PCR with V4-V5 primer set 515 F (5-GTGYCAGCMGCCGCGGTAA-3) and 926 R (5-CCGYCAATTYMTTTRAGTTT-3); one-step PCR using the TruFi DNA Polymerase; purified using AMPure XP beads; quantified and pooled.