title: piRNA-Seq of homo sapiens
design: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA) following the manufacturers procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0. Approximately 1 ug of total RNA were used to prepare small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). And then we performed the single-end sequencing (50 bp) on an Illumina Hiseq2500 at the OBIO (ShangHai, China) following the vendors recommended protocol.