title: WGS of Colletes collaris: adult male chest
design: Sequencing libraries were generated using Truseq Nano DNA HT Sample preparation Kit (Illumina USA). The DNA sample was fragmented by sonication to a size of 350bp, then DNA fragments were end polished, A-tailed, and ligated with the full-length adapter for Illumina sequencing with further PCR amplification. At last, PCR products were purified (AMPure XP system) and libraries were analyzed for size distribution by Agilent2100 Bioanalyzer and quantified using real-time PCR.