title: GSM7648550: lps1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA purity and quantification were identified using a NanoDrop 2000 spectrophotometer (Thermo Scientific, USA), RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions. Transcriptome libraries were constructed using the VAHTS Universal V5 RNA-seq Library Prep kit according to the manufacturer's instructions. Transcriptome sequencing and analysis were performed by Shanghai Ouyi Biotechnology Co., LTD (Shanghai, China).