title: GSM8171481: Kmt2d +/+ line 3, D7, exp2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were homogenized with TRIzol reagent, followed by phenol-chloroform separation. Total RNA was purified from the aqueous phase using the Zymo RNA Clean and Concentrator-5 kit with DNase I treatment. Library preparation was performed with the NEBNext Ultra II RNA Library Prep kit in conjunction with NEBNext Poly(A) mRNA Magnetic Isolation Module.