title: GSM6761961: F1_pre-cDC1-2; Mus musculus; ATAC-seq
library_construction_protocol: 5.0 × 104 cells were lysed in ice-cold ATAC-Resuspension Buffer (RSB) containing 0.1% NP40, 0.1% Tween-20 and 0.01% digitonin at 4 °C for 3 min, then washed with ATAC-RSB containing only 0.1% Tween-20. Nuclei were spun down by centrifugation and incubated in 50 mL of transposition buffer (25 mL 2×TD buffer, 16.5 mL PBS, 0.5 mL 1% digitonin, 0.5 mL 10% Tween-20, 5 mL H2O, 2.5 mL TDE1 Tagment DNA Enzyme (Illumina Tagment DNA TDE1 Enzyme and Buffer Kit, Illumina)) at 37 °C for 30 min in a thermomixer with 1000 rpm mixing. Transposed DNA was purified with a DNA Clean & Concentrator Kit (Zymo Research). Libraries were prepared with a Nextera DNA Library Prep Kit.