title: GSM8432953: DSP-1001660016238-F-E01; Mus musculus; OTHER
library_construction_protocol: One brain hemisphere was fixed in 4% paraformaldehyde (PFA) for 24 h, cryoprotected in escalating sucrose gradients, embedded, and cryosectioned in coronal plane sections onto histologic slides [10 μm thickness for spatial transcriptomics sections, 14 μm thickness for subsequent fluorescent in situ hybridization (FISH)] and stored at –80 °C. Spatial transcriptomics was performed by GeoMx DSP (NanoString, Seattle, WA), a platform for spatially analyzing gene expression in tissue in a highly multiplexed manner Other: Spatial transcriptomics-Nanostring GeoMx DSP