title: 16S rRNA v4 region gene amplicon sequencing data from Lepus americanus feces
design: Total DNA was extracted from 50 mg of dried ground feces for every individual and for a negative control using the QIAamp Powerfecal DNA Kit (Cat. 12830, Qiagen). DNA extracts were quantified using the Quant-IT PicoGreen Kit (Cat. P7589, Invitrogen) and sent to Genome Quebec (Montreal, Canada) for library preparation and Illumina sequencing (MiSeq PE, 250 bp) of the V4 region of the 16S rRNA gene using universal primers 515F and 806R (Walters et al., 2015).