title: GSM8377797: 2118R; Mus musculus; RNA-Seq
library_construction_protocol: Islets were isolated using the standard collagenase/protease digestion method. The pancreatic duct was cannulated and distended with 4°C collagenase/protease solution. Islets were separated from the exocrine tissue using Histopaque-1077. Hand-picked islets were cultured overnight at 37°C in RPMI-1640 media containing 10% FBS and 1% Penicillin/Streptomycin. Cells were dissociated using accutase digestion. RNA libraries were prepared for sequencing using standard 10X Genomics protocols