title: GSM8616980: hiPSC-ExN CTCF deletion bio rep 2; Homo sapiens; Hi-C
library_construction_protocol: HiC libraries were generated using the Arima-HiC+ kit (P/N A101020) according to the manufacturer's protocol. Briefly, differentiated ExNs were fixed in culture plates with 2% PFA (Fisher Scientific F79-500) at room temperature for 10 min. About 0.5 million cells were used as input for each HiC library. The crosslinked chromatin DNA was treated with restriction digestion, biotinylation, proximal ligation. The proximally ligated chromatin was sheared with a Covaris S220 sonicator to fragments between 300 and 1,000 bp. The shared chromatin was then indexed with the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences, 21024) and amplified with KAPA Library Amplification Kit (Roche, KK2620). The final libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced on NovaSeq with paired-end 100bp sequencing.