title: GSM8128162: Sample patient pool 1; Homo sapiens; RNA-Seq
library_construction_protocol: Single cell capture, cDNA amplification, and library construction were completed using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10x Genomics, cat#1000127, 1000128) according to manufacturer's protocols. SPRIselect Reagent (Beckman Coulter, cat#B23317) was used for cDNA clean up, and quality control steps were completed using Bioanalyzer High Sensitivity DNA Kits (Agilent, cat#5067-4626). Two 10x Genomics Single Cell Chromium 3' mRNA libraries were made (S1 and S2) and sequenced as part of one Illumina NovaSeq S1 run. Each sample had a sequencing yield of greater than 399 million reads. The sequencing run was performed using recommended parameters with 28 cycles + 90 cycles asymmetric run.