title: GSM7888157: PDO Day 28; Homo sapiens; RNA-Seq
library_construction_protocol: Tumor pieces and PDOs were dissociated with Trypsin, centrifuged, then resuspended in 0.1% BSA in PBS and strained through a 30 µm filter. Cells were then centrifuged and resuspended in freezing medium consisting of PDOs medium supplemented with 10% Fetal Bovine Serum (Gibco, 10270106) and 10% DMSO and placed in cryovials (10X Genomics.com, 2021). Transcriptome single-cell datasets have been generated using the Chromium Next GEM Single Cell 3´v3.1 (10X Genomics)