title: ChIPseq H3 of Arabidopsis thaliana : asf1ab Control
design: ChIP was performed from 1g of 3-week-old seedlings. Chromatin shearing was done using the COVARIS system with 130l Covaris microTubes and the following settings to achieve fragments of mononucleosomal size: Intensity: 5, Duty cycle: 15%, Cycles/burst: 300, Duration: 420 sec, Mode: frequent sweeping.To each Arabidopsis IP and corresponding inputs, 10% of Drosophila chromatin was added. ChIP-seq libraries were generated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs), from 10 ng of starting material. Sequencing was performed on a HiSeq2500, with paired-end reads of 50 bp length.