title: scRNA-seq using PB10X - VDJ of Homo Sapiens: jurkat cells
design: The PB10X protocol for cDNA generation is described in detail in the paper. The PB10X-compatible cDNA libraries were further processed with the Chromium Single-cell Human TCR Amplification kit PN-1000252 (10X Genomics), the 10X Library Construction kit PN1000190 (10X Genomics), and the Dual Index TT Set A PN-1000215 (10X Genomics), following the 10X 5 V(D)J Amplification and V(D)J Dual Index Library construction protocol. The required volumes were halved while maintaining all prescribed concentrations as specified in the protocol. 0.5 ng of cDNA per pool was used as input for the first V(D)J amplification step. The Human T Cell Mix 1 and 2 v2 (10X Genomics) were utilized specifically for V(D)J amplifications. The first V(D)J amplification was carried out using 12 cycles, while the second V(D)J amplification was carried out using 10 cycles. For the V(D)J library construction, a minimum input of 6 ng V(D)J amplified cDNA was employed, and a total of 8 cycles was applied for the sample index PCR. The PB10X libraries were sequenced across two AVITI sequencer runs, reading 26 nt for read 1, 90 nt for read 2, 10 nt for P7 index and 10 nt for P5 index.