title: GSM6928667: Parental 231 cells, rep 1; Homo sapiens; ATAC-seq
library_construction_protocol: Briefly, 250,000 cells were trypsinized and pelleted, washed once with cold 1X PBS, resuspended, and lysed in 50µL of ATAC-lysis buffer (ATAC Resuspension buffer: 10mM Tris-HCl pH 7.4, 3mM MgCl2, 10mM NaCl containing 0.1% NP40, 0.1% Tween-20 and 0.1% Digitonin), and incubated for 3 to 4 minutes on ice. Lysis was quenched with 1mL of wash buffer (ATAC-resuspension buffer containing 0.10% Tween-20), lysed nuclei were counted using Countess II cell counter. Nuclei were pelleted and resuspended in transposition mixture using an in-house prepared Tn5 transposase as described by Picellie et al. 2014.