title: GSM8376412: PBMCs, Batch 8, Feature barcodes; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs Samples labeled with TotalSeq C hashtag antibodies were pooled together to generate mixtures of 5 samples per batch (total 8 batches). Pooled samples were loaded into a 10X Genomics microfluidic platform (Single Cell 5' v2 Reagent Kit), targeting 12,000 – 16,000 cells per batch. Sequencing was performed on the NovaSeq6000 S4 flow cell targeting 50,000 reads per cell for the gene expression libraries and 5,000 reads per cell for the feature barcode libraries. Libraries were processed according to manufacturer's instructions. 10X scRNA-seq using 5' v2 chemistry