title: ONT direct cDNA of Puccinia striiformis f. sp. tritici isolate 104 E137 A-: 6 dpi, replicate 4
design: Snap freeze infected wheat leaves followed by RNA extraction, poly-A enrichment, 1st and 2nd strand cDNA synthesis. Sequenced on FLO-PRO114M flowcells. Sequenced at Biomolecular Resource Facility at the Australian National University. Basecalled with dorado dna_r10.4.1_e8.2_400bps_sup@v4.2.0