title: GSM8377632: tomato fruit pericarps, slalkbh2-25, rep1; Solanum lycopersicum; RNA-Seq
library_construction_protocol: Total RNA was extracted by using the hot-phenol method from the wild-type and slalkbh2 mutant (slalkbh2-25 and slalkbh2-28) fruits at the mature green (MG) stage. mRNAs were isolated from the total RNA and then fragmented, followed by RNA-seq library construction. For library construction, 50 ng of fragmented mRNAs were used for library construction with NEBNext ultra RNA library prepare kit for illumina (NEB, E7530). High-throughput sequencing was performed on the illumina HiSeq X sequencer with a paired-end read length of 150 bp according to the standard protocols.