title: GSM8619174: donor_3350_visceral fat; Homo sapiens; RNA-Seq
library_construction_protocol: Adipose tissue biopsies with at least 1g  were obtained from adult patients without severe morbid states or obesity complications who had signed in advance a written informed consent before undergoing elective abdominal surgeries. Five patients donated subcutaneous adipose tissue and visceral adipose tissue samples (paired). Tissues were immediately delivered from the operating room to the lab, where they were snap frozen in liquid nitrogen and stored at −80°C. 13,000-16,500 nuclei/sample were  loaded onto 10X Chromium Controller (10X GENOMICS, CA, USA) according to the manufacturer's protocol (Chromium Next GEM Chip C. Chromium Next GEM Single Cell 3' Kits v3.1).