title: GSM8301033: HC, replicate3, scRNAseq; Macaca fascicularis; RNA-Seq
library_construction_protocol: First, 3 mL of Ficoll solution (Lymphoprep, Stemcell Technology) was added to a 15 mL centrifuge tube. The centrifuge tube was tilted, and the bone marrow (less than 3 mL) was transferred to the top of the Ficoll solution against the wall of the centrifuge tube. Next, the centrifuge tube was centrifuged at 800 × g for 20 minutes (acceleration 6, brake 2) at room temperature. Finally, the BMMC at the interface were carefully harvested. The isolation BMMC were captured by the 10X genomics chromium Single Cell 5' solution. Then, reverse transcription was performed on drops and the cDNA was amplified following multiple amplification schemes from 10X genomics, including extension, purification, adapter connection. Sequencing libraries were quantified using the Qubit High Sensitivity DNA assay and NGS3K assay. The library was sequenced by Novaseq illumina (Illumina, San Diego, CA) on a 150 bp paired-end run. All the single cell library preparation was according to the 10X genomics Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index).