title: GSM8301149: TNBC_0182; Homo sapiens; RNA-Seq
library_construction_protocol: TNBC tissues from African American AA and EA women with or without obesity from Louisiana were used as source of RNA. Covaris kits (truXTRAC FFPE total NA (column)) were used for RNA extraction following the manufacturer's instructions. The RNA was treated with DNaseI, quantified by Qubit (Invitrogen), and qualified high sensitivity RNA Agilent chips Genomic libraries were constructed using the Illumina TruSeq RNA Exome protocol. Briefly, 300 ng of total RNA (DV200≥30) were fragmented and used as template for the generation of double-stranded cDNA to which adapters were ligated. DNA fragments were enriched by PCR and the size of the fragments checked on Agilent Bioanalyzer 2100 DNA chips. Exome regions are captured, cleaned, pooled and enriched by PCR. Finally, the library quality and concentration are checked and diluted to the suggested final concentration for sequencing in an Illumina NextSeq500 at 2 x 75 bp