title: GSM7888229: CVS_F_71702; Homo sapiens; RNA-Seq
library_construction_protocol: Placenta tissue was submerged in RNAlater RNA stabilization reagent (QIAGEN) and stored at -80C until further processing. Tissue was thawed on ice and homogenized using sonication and/or by passing tissue through needles, then total RNA was isolated using the AllPrep DNA/RNA/miRNA Universal kit (QIAGEN). Polyadenylated RNA was selected during the library preparation process. For library preparation, 1 ug of the total RNA elution was used with the Illumina TruSeq Stranded mRNA library preparation kit (Illumina), with polyA mRNA selection then cDNA synthesis using SuperScript II reverse transcriptase (Invitrogen) and random primers. The cDNA was converted into double stranded DNA and PCR-amplified, then purified with Agencourt AMPure XP beads (Beckman Coulter). Sample libraries were multiplexed and sequenced on a NovaSeq 6000 platform (Illumina) using 75bp single-end mRNA-sequencing, with average 30 million reads per sample.