title: GSM6670934: 3 dpa, scRNA-seq; Danio rerio; RNA-Seq
library_construction_protocol: Tissue from 20 adult zebrafish was pooled for each group. Regenerative caudal fin tissue was collected into 1x PBS on ice, diced, then washed with L-15 media supplemented with 1X GlutaMax, 1X Antibiotic-Antimycotic and 1X Penicillin-Streptomycin (all Thermo Fisher). The tissue was then enzymatically digested using 0.25% Trypsin-EDTA (Thermo Fisher) and Liberase DL (2 mg/ml, Sigma) at room temperature for 20 min followed by mechanical dissociation by pipet titration every 5 minutes. Enzymatic digestion was inhibited by the addition of 20% fetal bovine serum. Cells were filtered through a 100 μm cell strainer and then pelleted by centrifugation at 0.2 g for 2.5 minutes. The cell pellet was washed with PBS and re-suspended in PBS + 0.04% bovine serum albumin. Library was performed according to the manufacturer'