title: Primary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Single cell RNAseq
design: Primary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Single cell RNAseq
library_construction_protocol: Hematopoietic cells expressing the ETO2-GLIS2 fusion were purified by flow cytometry including for cell viability (using Sytox blue-negative cells). Sample preparation was done at room temperature. Single-cell suspensions were loaded onto a Chromium Single Cell Chip (10x Genomics) according to the manufacturer's instructions for co-encapsulation with barcoded Gel Beads at a target capture rate of ~10,000 individual cells per sample. Captured mRNAs were barcoded during cDNA synthesis using the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 (10X Genomics) according to the manufacturer's instructions. All samples were processed simultaneously with the Chromium Controller (10X Genomics) and the resulting libraries were prepared in parallel in a single batch.