title: degradome sequence
design: Poly(A) RNA was purified from total plant RNA (20ug) using poly T oligo-attached magnetic beads after two rounds of purification. The 5 adapters were ligated to the 5 end of the 3 cleaved mRNA fragments from miRNA-induced cleavage by RNA ligase. Bioti-nylated Random Primers and mRNA were mixed for reverse transcription and amplified by PCR to construct a cDNA library with an average insert size of 200-400 bp. Finally, we performed the 50 bp single-end sequencing on an Illumina HiSeq 2500 (LC Bio, China) following the vendors recommended protocol.