title: GSM7796953: L1 GC n2; Homo sapiens; miRNA-Seq
library_construction_protocol: RNA was isolated directly from extracellular vesicles using the miRNeasy Serum/Plasma Advanced Kit (QIAGEN, #217204) and following the vendor's instructions. The SMARTer® smRNA-Seq Kit for Illumina® (Takara (Tokyo, Japan), #635030) was used and vendor's instructions were followed with slight modifications. The libraries were then bead-purified, by  incubating with AMPURE beads (Beckmann Coulter (Brea, California, USA), #A63881).  For size exclusion, a similar protocol was repeated using SPRI beads (Beckmann Coulter, #B23318) and a sample-to-beads ratio of 1:1.  The libraries were sequenced on a Nextseq550 mid output 150 cycles v2.5 flowcell spiking in 30% PhiX control v.3.