title: GSM8127757: β-Thalassemia (Hbb[th3/+]), single haplotype, bone marrow, replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow and spleens were harvested from mice of the following genotypes: WT sing, WT diff, HbbTh3/+ sing, HbbTh3/+ diff. For each sample Ter119+ cells were isolated from 3 x 107 live bone marrow or spleen cells respectively with Anti-Ter-119 MicroBeads, mouse (Miltenyi, 130-049-901) on MS Columns (Miltenyi Biotec, 130-042-201). Total RNA was purified from Ter119+ cells with the RNeasy Plus Mini Kit (Qiagen, 74134) following the manufacturer's instructions. Libraries were prepared with the TruSeq Stranded Total RNA Library Prep Gold Kit (Illumina, 20020599) according to the manufacturer's instructions.