title: GSM7796030: WT + DMXAA [EV_D_4]; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Quick-RNA miniprep kit (Zymo Research). Greater than 1 microgram of RNA was sent for RNA sequencing. Libraries were constructed at Novogene. polyA-tailed mRNA was enriched with olig(dT) beads. mRNA was randomly fragmented with fragmentation buffer and cDNA was synthesized with random hexamer primers prior to second strand synthesis (Illumina). Terminal repair, A ligation, and sequencing adaptor ligation were performed prior to size selection and PCR enrichment.