title: GSM6636959: A549_Mock_biolrep1; Homo sapiens; RNA-Seq
library_construction_protocol: Samples for bulk RNA sequencing were lysed in Trizol Reagent and total RNA was extracted using the miRNeasy mini kit (Qiagen) per the manufacturer's instructions. DNAse treatment was performed on isolated RNA using the RNA Clean and Concentrator Kit (Zymo). Total RNA was examined for quantity and quality using the TapeStation (Agilent) and Quant-It RNA (ThermoFisher) systems. RNA samples with sufficient material (10 pg–10 ng) were passed to whole-transcriptome library preparation using the SMART-Seq v4 PLUS Kit (Takara Bio) following the manufacturer's instructions. Briefly, total RNA inputs were normalized to 10ng in 10.5 µl going into preparation. 3' ends of cDNA were then adenylated prior to ligation with adapters utilizing unique dual indices (96 UDIs) to barcode samples to allow for efficient pooling and high throughput sequencing. Libraries were enriched with PCR, with all samples undergoing 14 cycles of amplification prior to purification and pooling for sequencing.