title: GSM6883993: eWAT, wildtype, ZT10, rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from tissues using TRI reagent (T9424, Sigma-Aldrich) and isolated using RNeasy Mini Kit (74106, Qiagen). From this, 500-1000 ng RNA was used for reverse transcription with the High Capacity cDNA Reverse Transcription kit (4368814, Applied Biosystems). RNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA with Ribo-Zero Gold protocol (Illumina)