title: GSM8127699: Lip_DETA_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using Quick-RNA MiniPrep Kit (Genesee Scientific: 11-328). When necessary, samples were further concentrated and purified with the RNeasy MinElute Cleanup Kit (Qiagen: 74804). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.