title: GSM8263547: CC-5325 strains, WT, mt–, vegetative stage, H3K27ac, ABCAM; Chlamydomonas reinhardtii; ChIP-Seq
library_construction_protocol: Cells were fixed with 0.35% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using Covaris S220. ChIP-seq libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina V3 (Cat. ND607, Vazyme). 1 ng of DNA was used as starting material for input and ip samples. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were size selected at 250-450bp in length using VAHTS DNA Clean Beads (Cat. N411, Vazyme).