title: GSM8171694: testis with normal spermatogenesis_1 (characterization of the hTP cells); Homo sapiens; RNA-Seq
library_construction_protocol: The cell samples were lysed in 350 µl of RLT buffer with β-mercaptoethanol and RNA, DNA and protein were extracted according to the manufacturer's protocol AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany). The RNA samples were additionally purified according to the manufacturer's protocol of Turbo DNA-free Kit (Thermo Fisher Scientific, MT, USA). Sequencing libraries of cell samples were prepared using the the TruSeq Stranded Total RNA with Ribo-Zero Human (Illumina) and sequenced on an Illumina NovaSeq6000  platform Paired end 150bp sequencing