title: GSM8672963: pituitary gland, WT; Danio rerio; RNA-Seq
library_construction_protocol: After dissection, zebrafish pituitaries from WT or amh-/- females were washed with cold PBS or fresh Ringer's solution for 5-10 min, followed by mechanical and enzymatic dissociation in protease solution (0.25% trypsin (Life Technologies), 400 mg/ml collagenase D (Sigma) and 1 mM EDTA in PBS) and incubated at 28.5 °C for 20-30 min or until full dissociation was observed. Dissociation was stopped by the addition of 6X stop solution (6 mM CaCl2 and 30% foetal bovine serum (FBS) in PBS). The samples were filtered with a 40 μm cell strainer, centrifuged (2000 rpm, 5 min, 4 °C) and then resuspended in 1X PBS. Single-cell cDNA libraries were synthesized using 10X Genomics Chromium Single Cell 3' Library & Gel Bead Kit v3.1 according to the manufacturer's instructions.