title: GSM6670648: U2OS, SoF K492A GR-HaloTag, 0hdex, rep1; Homo sapiens; OTHER
library_construction_protocol: RNAs labeled with 4-thiouridine (4sU) were isolated as described previously (Methods Mol Biol. 2017;1648:169-176. doi: 10.1007/978-1-4939-7204-3_13). Briefly, TRIzol was applied to cell dishes to lyse and extract RNA. Total RNA was subjected to biotinylation to modify 4sU-labeled RNAs. The biotinylation reagent used was biotin-MTSEA-XX at 0.1 mg/mL (Biotium). Biotinylated RNAs were isolated using magnetic streptavidin beads. Libraries were prepared using the KAPA RNA HyperPrep kit with RiboErase. Adapters used were IDT xGen UDI-UMI Adapters.