title: GSM8218087: Male RISP knockout_1; Mus musculus; RNA-Seq
library_construction_protocol: Heart tissue was shredded to disrupt the cells (QIAshredder catalog no. 79656, QIAGEN) allowing total RNA to be isolated (RNeasy mini kit, catalog no. 74104, QIAGEN) RNA-seq libraries were generated (NEB Next RNA Ultra library preparation kit with poly(A) enrichment module) from 500 ng of total RNA. RNA-seq- libraries were pooled and sequenced on a NextSeq 500 instrument (Illumina) using the High Output 75 cycles V2 kit to an average depth of 10M reads per sample.