title: PacBio HiFi of Galeolaria caespitosa sperm
design: High-molecular-weight genomic DNA was extracted from sperm using Qiagens Blood & Cell Culture DNA Kit and protocol. DNA yield and mean fragment size were assessed using a Qubit fluorometer and pulsed-field gel electrophoresis, respectively, and purity was confirmed using 260/280 and 260/230 absorbance ratios on a Nanodrop spectrophotometer. A PacBio HiFi library was then prepared using PacBios SMRTbell Express Template Prep Kit and protocol, and sequenced in two SMRT Cells on the PacBio Sequel II platform (Cantata Bio, California, USA) to ~59x sequence coverage.