title: GSM8673007: GFP_liver_infected_Ec2; Mus musculus; RNA-Seq
library_construction_protocol: Neonatal livers were dissociated using the same instrument with the “37C_m_LIDK_1” protocol and the Miltenyi Biotec murine liver dissociation kit. Following dissociation, tissues were pelleted at 350 x g for 5 min at 4˚C and ACK lysed for 4 min at room temperature. ACK was neutralized with 10% FBS in RPMI and pelleted at 350 x g for 5 min. Cell debris was removed by putting cells through 40 µm strainer in a 50 mL conical tube and pelleted. Cells were counted and assessed for viability using the ThermoFisher Scientific Countess 3 Automated Cell Counter (Waltham, MA, USA). Downstream steps were performed according to the 10X Genomics manufacturer recommendations for the 10X Genomics Chromium Next GEM Single Cell 3' Kit v3.1 (Product 1000269). The library was prepared using the 10X Genomics Chromium Controller available in the WVU FCSCCF by the WVU Genomics Core Facility.