title: GSM8217330: WT; Mus musculus; RNA-Seq
library_construction_protocol: Briefly, mice were anesthetized with intraperitoneal Avertin injection (1 mL/40 g body weight) and the pancreas was infused with 3 mL of a collagenase P solution (Sigma Chemical, St. Louis, MO; 0.5 mg/mL in HBSS). The pancreases were subsequently removed and incubated at 37°C for approximately 12 minutes until complete digestion was achieved. Digestion was stopped by adding 30 mL of HBSS, followed by three washes of 20 mL HBSS. The isolated islets were carefully selected under a stereoscopic microscope using a pipette and then cultured in RPMI-1640 supplemented with 10% FBS and 100 U/mL penicillin-streptomycin. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.