title: GSM7796973: 2nd_allmixed,biol rep1,scRNAseq,ADT_HTO; Mus musculus; OTHER
library_construction_protocol: CD45+ hematopoietic and CD45- nonhematopoietic cells were FACs-sorted from the ears of either naïve or infected WT and eoCre : Il4/13f/f mice and mixed at 9:1 ratio (CD45+:CD45-),  and then processed through the Chromium Single cell 3' v3.1 Library kit (10x Genomics) per the manufacturer's protocol. Library preparation was performed according to the manufacter's instructions (single cell 3' v2 protocol, 10x genomics). Briefly, 8,300 sorted cells were loaded for GEM formation, targeting 5,000 single cells for analysis from each ear. The RNAs from lysed single cells in gel beads were reversed transcribed into cDNA with Barcodes. Library fragment-size distribution was assessed using the Bioanalyzer 2100 and the DNA high-sensitivity chip (Agilent Technologies), followed by amplification, fragmentation, adapter ligation, and size selection to generate both 3' gene expression libraries and cell surface protein libraries.