title: NC8_1
design: Libraries were built from 250 ng of total RNA using the NEBNext Multiplex Small RNA Library Prep Set for Illumina following manufacturers recommendations (New England Biolabs). Thirteen PCR cycles were completed to finalize the library preparation. The resulting indexed libraries were size-selected and purified using SPRI beads (Beckman-Coulter) with 1X ratio. Quantification and quality assessment of each library were performed with Qubit 4.0 (HS DNA kit, Thermofisher) and 4150 Tapestation analyzer (D5000 ScreenTape kit, Agilent) respectively. Libraries were pooled in an equimolar manner and sequenced on an Illumina NextSeq 500 sequencer with HighOutput reagents. A total of 420M sequence reads were obtained with 63M to 73M of reads per sample.