title: mWGS sequencing of rhizophere microbiome from rye grown in colluvial Gleyic-Colluvic Regosol (replicate A) at pre-flowering
design: Genomic DNA was obtained from 0.4 g of the rhizosphere pellet using the DNeasy PowerLyzer PowerSoil Kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer instructions. The lyses step was performed with a FastPrep-24 bead beater at a speed of 3400 rpm (MP Biomedical, LLC Goddard Irvine, CA, USA)