title: GSM8263838: H_F1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated Cells were washed twice with cold PBS and subsequently lysed in TRIzol (Invitrogen) and stored at -20 C. RNA isolation was performed according to the TRIzol RNA isolation protocol entirely for qPCR samples or until the phase separation step for the RNA-sequencing samples. Subsequently, for these samples the aqueous phase (containing the RNA) was transferred to an RNeasy mini kit (Qiagen) column to continue RNA isolation. Subsequently, RNeasy RNA isolation protocol was followed from the addition of 70% ethanol onwards. DNA contamination was removed using TURBO DNA-free Kit (Invitrogen) according to protocol. RNA sample quality control, mRNA library preparation (poly A enrichment) and sequencing (Illumina NovaSeq PE150) was performed by Novogene