title: GSM6761407: KO-H4h Input; Mus musculus; ChIP-Seq
library_construction_protocol: Three weeks old female mice were intraperitoneally injected with 5 IU of PMSG (Ningbo San Sheng Biotech, Ningbo, China) for 48 h to stimulate follicle development, followed by injection with 5 IU human chorionic gonadotropin (HCG; Ningbo San Sheng Biotech) to induce ovulation. Fresh ovary granulosa cells were extracted from mice ovary and a ChIP assay was performed using a Simple ChIP® Enzymatic Chromatin IP Kit (9003, Cell Signaling Technology) in accordance with the manufacturer's instructions. The CHIP DNA was assessed by Agilent 2200 TapeStation(Agilent Technologies, USA) and Qubit(Thermo Fisher Scientifc,USA) .The ChIP-Seq libraries were constructed following the Illumina ChIP-Seq library gener- ation protocol. Briefly, chip DNA was fragmented to approximately 200bp , Subsequently, the fragments were repaired end and added A-tailing following by adaptor ligation. The adaptor-ligated DNA was enrichment with a low-cycle according to instructions of NEBNext® UltraTMDNA Library Prep Kit for Illumina (NEB, USA). The DNA library products were assessed using the Agilent 2200 TapeStation(Agilent Technologies, USA) and Qubit (Thermo Fisher Scientific, USA), the libraries were then sequenced with pair-end 150 bp by Illumina platform  (Illumina,USA)