title: Ahemp_genome
design: High molecular weight genomic DNA (HMW gDNA) was extracted from a coral nubbin of A. hemprichii preserved in DESS buffer using the Qiagen genomic tip 100/g kit following the manufacturers instructions. High quality HMW gDNA was used to construct a short-reads library utilizing the NEBNext Ultra II DNA kit following the manufacturers instructions. Briefly, 1 g of gDNA was fragmented by Adaptive Focused Acoustic technology (Covaris, Woburn, Massachusetts, USA) to a target size of 300 bp to achieve overlapping paired-end reads. The fragmented DNA was end-repaired and dA-tailed for Illumina adapter ligation. Adapter-ligated DNA was purified and size selected for fragments within the 300-400 base pair range using AMPURE XP magnetic beads (Beckman Coulter, Brea, California, USA). The resulting product was subjected to PCR amplification using the NEBNext primer cocktail and Q5 Master Mix. The final library was quality-checked by assessing both its size (~400 bp) using a 2100 Bioanalyzer (Agilent, Santa Clara, California, USA) and its concentration using a Qubit fluorometer and the Qubit High Sensitivity dsDNA assay kit (Thermo Fisher Scientific, Waltham, Massachusetts, USA). The paired-end library was shipped to the Macrogen facilities to be sequenced on one Illumina HiSeq2500 lane (rapid run 2 x 251 bp).