title: GSM7360701: Control, 16hr , CT2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Rneasy mini kit (Qiagen). 1.5μg of total RNA was used for the construction of sequencing libraries. RNA integrity was checked using Agilent Technologies 2100 Bioanalyzer. (poly (A) tail containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification. Following purification, poly (A) RNA was fragmented using divalent cation buffer in elevated temperature. Poly(a) RNA sequncing library was prepared using Illumina's Truseq-stranded mRNA sample preparation protocol. Quality control analysis and quantification of the sequencing library were performed using Agilent Technologies 2100 bioanalyzer high sensitivity DNA chip. Paired-ended sequencing was performed on Illumina's Novaseq 6000 sequencing system.