title: GSM7868391: OBR1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated by Trizol, followed by precipitation using absolute alcohol and cleaned-up using Quick-RNA miniprep kit (Zymo Research). Samples were prepared following the NEB Next stranded RNA with the Poly(A) mRNA magnetic isolation module (NEB #E7490). Samples were standardized to 160 ng input, with 15 minutes fragmentation, no size selection, 1:30 adapter dilution, and 13 PCR cycles. Samples were pooled equimolarly and size selected with a 0.6x bead ratio. Samples were sequenced on the Illumina NextSeq 2000 platform with a 100 paired end sequencing program on a P2 flowcell.