title: GSM7528302: primary cells, lymph nodes [Rewild2_GEX]; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspension from the mesenteric lymph nodes (MLNs) were prepared by mashing the tissues individually through a 70μm cell strainer and washed with RPMI. Cells were then washed with RPMI supplemented with 10% FCS. Library was performed according to the manufacter's instructions (10x Genomics). Briefly, pooled samples from each group were then loaded on a 10X Genomics Next GEM chip and single-cell GEMs were generated on a 10X Chromium Controller. Subsequent steps to generate cDNA and sequencing libraries were performed following 10X Genomics' protocol. Libraries were pooled and sequenced using Illumina NovaSeq SP 100 cycle as per 10X sequencing recommendations.