title: scRNA-seq of Mus Musculus Raji-bearing mouse spleen CD45+ cells (CAR-Toci treatment)
design: Spleen samples were harvested from Raji tumor-bearing humanized NSG-SGM3 mice (pooled from one male, one female). Tissues were homogenized to single-cell suspensions then subjected to magnetic bead-based cell sorting to isolate CD45+ cells. Single-cell RNA sequencing libraries were prepared using a 10x Genomics Chromium Controller, and libraries were sequenced on the Illumina NovaSeq SP platform with 50-bp paired-end reads.