title: GSM8263483: miRNC_1; Homo sapiens; RNA-Seq
library_construction_protocol: TOTAL RNA was extracted from the tissue samples, the concentration and purity of the extracted RNA was examined using Nanodrop2000, RNA integrity was examined by agarose gel electrophoresis, and the Agilent5300 determined the RQN values were determined by Agilent5300. For single library construction, the total RNA amount was 1 μg, the concentration was ≥ 30 ng/μL, the RQN was > 6.5, and the OD260/280 was between 1.8 and 2.2. Total RNA was first extracted and mRNA was enriched by Oligo dT, then fragmented mRNA was synthesized into cDNA by reverse transcription, ligated into adapter, and sequenced by NovaSeq X Plus platform after fragment screening and library enrichment.