title: GSM8377722: si-NC_H3K27ac, CUT&Tag seq; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag assay was performed using NovoNGs CUT&Tag 4.0 High-Sensitivity Kit following the manufacturer's instructions. Briefly, a total of 1×105 cells were harvested and bound to ConA magnetic beads. The cells were incubated with primary H3K27ac antibody or primary H3K4me1 antibody at 4 ℃ overnight, and then incubated with Goat Anti-Rabbit IgG H&L at room temperature for 1 h. After washing with wash buffer, the cells were incubated with pAG-Transposome at room temperature for 1 h. Subsequently, the cells were washed with wash buffer, incubated with tagmentation buffer at 37℃ for 1 h, and then the reaction was terminated with stop buffer at 55℃ for 10 min. DNA was extracted using the tagment DNA extract beads. PCR was performed with index primers using extracted DNA to construct CUT&Tag library. The CUT&Tag libraries were performed sequencing using an Illumina NovaSeq 6000 platform.