title: GSM7868739: MM1S BRD4 1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked for 10 min with 1% formaldehyde at 37C. This reaction was subsequently quenched with 125 mM glycine for 5 min and 5-10 million fixed cells were used for the ChIP experiments. Chromatin from fixed cells was fragmented by sonication with a Covaris E220, and the solubilized chromatin was incubated with the indicated antibody overnight at 4C. Antibody-chromatin complexes were pulled down by incubation with Protein G-Dynabeads (Thermo Fisher Scientific) for 3 hours at 4C, washed, and eluted. The samples then underwent crosslink reversal, RNase A (Roche) treatment, and proteinase K (Thermo Scientific) treatment before the captured DNA was extracted using AMP Pure beads (Beckman Coulter) ChIP-seq libraries were made using the Ion Plus Fragment Library Kit (Thermo Fisher).