title: GSM6928226: WT_m6A MeRIP-seq_rep1; Arabidopsis thaliana; RIP-Seq
library_construction_protocol: Total RNA was extracted from Arabidopsis seedlings using Trizol (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instruction. Poly(A) mRNA was extracted from total RNA using a Dynabeads Oligo (dT)25 kit (Thermo Fisher, Waltham, MA, USA). The RNA was then fragmented into oligonucleotides using a Magnesium RNA Fragmentation Module (NEB), the fragmented RNA was incubated with m6A-specific antibody (Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl, and 0.5% Igepal CA-630), and the bound RNA was recovered. The RNA from IP fraction and input control fraction was converted to a cDNA library, and the paired-end libraries with an average insert size of approximate 100±50 base pair were subjected to sequencing