title: HS-2801
design: DNA extracted from tissue using BioSprint automated DNA extraction, quantified by Qubit, normalized to 20 ng/ul, then used for double-digest RAD-sequencing using enzymes NsiI and MspI. Libraries were sequenced in batches of 20 samples per sequencing chip on an Ion Torrent Proton sequencer. Quality trimming and adapter removal was done by cutadapt, and demultiplexing was conducted using process_radtags module of Stacks2. In cases where samples were sequenced on multiple chips, all data was combined into a single fastq file per sample. See manuscript for more details and for citations for tools applied.