title: GSM7468687: cut-and-run_FOXM1_rep1; Homo sapiens; OTHER
library_construction_protocol: CUT&RUN assay libraries for primary renal proximal tubular epithelial cells  were generated with the CUTANA kit (EpiCypher, 14-1048). 500,000 cells were mixed and incubated with Concanavalin A (ConA) conjugated paramagnetic beads. Antibody to FOXM1 was added to target samples and IgG was added to negative controls. The remaining steps were performed according to manufacturer's instructions. Library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, E7645S) with manufacturer's instructions, including minor modifications indicated by CUTANA described above. cut-and-run with CUTANA kit (EpiCypher, 14-1048)