title: GSM8561368: M13 phage, NNK codon encoded cyclic peptide library, against AB42, no replicate; Inovirus M13; OTHER
library_construction_protocol: M13 phage pool in 2xYT medium was used directly as template for PCRs M13 phage pool was directly used as template in PCR using appropriate primers specified in this study. Q5 DNA polymerase (from NEB) and buffer were used. Minimal number of cycles was used in PCR reactions to amplify gene of interest from the M13 phage genome. PCR products were purified and pooled as libraries. Amplicons for NGS using Illumina NovaSeq 6000