title: Tagmentation sequencing of DRT2-expressing E. coli: uninfected cells
design: DNA was extracted by pelleting cultures followed by alkaline lysis and column purification (Qiagen Spin Miniprep Kit). DNA (100 ng for each sample) was mixed with 10 ng of spike-in DNA (pMW342, Table S1) and tagmented with Tn5, amplified with barcoded primers, and sequenced using a MiSeq in single-end mode (250 bp read 1).