title: L3MBTL3 ChIP-seq of homo sapiens: breast cancer cell SUM159PT
design: Cells were cross-linked using 1% formaldehyde and sonicated to generate DNA fragments of about 500bp. For immunoprecipitation, Dynabeads Protein A or G magnetic beads (Invitrogen) were incubated with antibodies and chromatin fraction of cell lysate overnight at 4 C with rotation. After washing, the protein-DNA complexes were eluted. The sequencing libraries were generated using NEBNext Ultra DNA Library Prep Kit from Illumina (NEB,USA) by following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using HiSeq Rapid Duo cBot Sample Loading Kit (Illumia) according to the manufacturer's instructions. The libraries were sequenced on the Illumina NovaSeq 6000.