title: Gut pool metagenome of Apis mellifera ligustica exposed to BLUE1 pigment and Glyphosate
design: The 16S rRNA gene was used as a phylogenetic marker to identify bacterial taxa of the gut microbiota using the short (470 bp) hypervariable regions V3-V4 of this conserved gene (Primer 341F: CCTAYGGGRBGCASCAG. Primer 806R: GGACTACNNGGGTATCTAAT). The amplification of the 16S rRNA gene, purification, library construction and high-throughput sequencing services were hired at Novogene Corporation Inc. (Sacramento, USA).