title: GSM7868635: RA12 SFMC ++; Homo sapiens; RNA-Seq
library_construction_protocol: High quality RNA was isolated from RA CD206+CD163+ and CD206-CD163- synovial tissue and fluid using the RNAqueous total RNA isolation kit (BD Biosciences and the purity of RNA samples was assessed using a NanoDrop 2000 spectrophotometer (ThermoFisher). RNA samples were reverse transcribed and sequence libraries were generated by Janssen Pharmaceutical, Spring House, PA, USA, using the NuGen Ovation Universal RNA-seq System. The resulting sequencing libraries were analysed using the Cali-per LabChip GX and quantified using KAPA qPCR.