title: Single-cell transcriptomic sequence of E15.5 mice: cortex tissue
design: Samples are first prepared into a single-cell suspension, followed by cell counting and cell viability measurements, if any 80%, adjust the cell concentration to 1000/l. The prepared cell suspension is encased in droplets by using a microfluidic chip, beads with cell barcode sequences and cells are collected, and the cells are lysed in the droplets, so that the mRNA in the cells is connected to the cell barcode on the bead to form Single Cell GEMs (Gel Bead-in-Emulsions), which are subjected to RT reaction in the droplets, and then demulsifiedto perform library construction of cDNA.