title: GSM8218100: MBO, Day 14, BA patient No.2, scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: MBOs were washed with DPBS and incubated with Accutase at 37 °C for 10 mins to dissociate into single cells. The dissociated cells were filtered thorough 30 µm cell strainer and resuspended in William's E medium containing 0.01% BSA. Libraries were constructed according to the the manufacter's instructions (Chromium Single Cell 3' Reagent Kit v3.1 protocol with Chromium platform, 10× Genomics), aiming for 10,000 cells. The barcoded cDNA was cleaned with Silane DynaBeads and amplified using 14 PCR cycles. Full-length, barcoded cDNA was then enzymatically fragmented, sized-selected using SPRIselect Reagent, adapter-ligated, and amplified for library construction. During the library construction, Illumina R2 primer sequence, paired-end constructs with P5 and P7 sequences, and sample indexes were added.