title: GSM7528398: iALI-Control+_scRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Trypsin was used during 15 minutes at 37°C, to dissociate iALI cells from Transwells. Dissociated cells were then filtered through a 40 μm porosity (Corning) on PneumaCult™-ALI Medium and then centrifuged (1200 rpm for 5 min). Cells were resuspended in 1,5 mL of PneumaCult™-ALI Medium and labeled with isotype-PE or CD326 antibody  during 30 min at 4°C. Cells were washed with media complemented with BSA (0,01%) and then sorted on BD FACSAria™, with MRI platform. 112 700 alive epithelial cells were collected and 10 000 cells were captured for single cell RNA sequencing. Libraries were constructed according to the manufacturer's instructions using Chromium™ Single Cell 3' v3.1 Reagent Kits and a Chromium instrument.