title: RNA-Seq of Anastrepha ludens: brown pupae male from GUA10 GSS
design: Total RNA was isolated using TRISure (Bioline Meridian Bioscience) and the Direct-zol-96 MagBead RNA kit (Zymo Research), followed by TURBO DNase (Invitrogen) treatment and clean-up with the RNA Clean & Concentrator-5 kit (Zymo Research). Libraries were preparation using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the Poly(A) mRNA Magnetic Isolation Module protocol, and sequenced on a AVITI platform (Element Biosciences) in a 150 bp paired-end run.