title: GSM8673249: E53 human metacarpal, digit 1, rep 1; Homo sapiens; ATAC-seq
library_construction_protocol: Embryonic tissue was microdissected under a light microscope in 5% fetal bovine serum in Dulbecco's Modified Eagle Medium (DMEM) on ice. The pooled phalanges (proximal and distal for digit I; proximal, intermediate, and distal for digits II-V) and metapodials were collected separately for all digits in both the fore- and hind limbs. The right and left sides of each element were pooled together. ATAC-seq protocol was performed as described in Young et al., 2022. After ATAC-seq, samples were then sent out for 1-2 rounds of sequencing on an Illumina NovaSeq 6000 at the Harvard Bauer Core in order to obtain at least 40 million reads per sample. ATAC-seq protocol was performed as described in Young et al., 2022.