title: GSM6761454: KO3; Mus musculus; RNA-Seq
library_construction_protocol: Three weeks old female mice were intraperitoneally injected with 5 IU of PMSG (Ningbo San Sheng Biotech, Ningbo, China) for 48 h to stimulate follicle development, followed by injection with 5 IU human chorionic gonadotropin (HCG; Ningbo San Sheng Biotech) to induce ovulation. Fresh ovary granulosa cells were extracted from mice ovary and used for RNA-seq Total RNA was isolated from cells/tissues using the Magzol Reagent (Magen, China)according to the manufac- turer's protocol, The quantity and integrity of RNA yield was assessed by using the K5500(BeijingKaiao, China) and the Agilent 2200 TapeStation (Agilent Technologies, USA) separately. Briefly, the mRNA was enriched by oligodT according to instructions of NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB,USA).And then fragmented to approximately 200bp. Subsequently, theRNA fragmentswere subjected to first strand and second strand cDNA synthesis following by adaptor ligation and enrichment with a low-cycleaccording to in- structions ofNEBNext® UltraTM RNA Library Prep Kit for Illumina. The purified library products were evaluated using the Agilent 2200 TapeStation and Qubit(Thermo Fisher Scientific, USA). The libraries were sequenced by Illumina (Illumina, USA) with paired-end 150bp