title: GSM8619266: HepG2 cell, PAOA, MPRA DNA barcode reads replicate 2; Homo sapiens; OTHER
library_construction_protocol: Cells were harvested using the Quick DNA/RNA Miniprep Kit (ZYMO, D7001) for simultaneous DNA and RNA extraction, with an additional DNase treatment (TaKaRa) to ensure DNA-free RNA. For each biological replicate, 240 µg of RNA and 48 µg of DNA were used for RNA/DNA-Lib construction, by using SuperScript II (for cDNA synthesis) and NEBNext® High-Fidelity 2X PCR Master Mix (for PCR amplification).