title: Genomic DNA, Illumina 150 bp paired-end reads, Bradysia coprophila adult X'X female-producing female heads, library 1, sequencing rep 1, Edinburgh
design: Genomic DNA was extracted using a version of the Qiagen DNeasy Blood and Tissue Kit extraction protocol modified for high-molecular weight (HMW) extractions and quantified using the Qubit (ThermoFisher). DNA from 50-60 pooled X'X heads was used for sequencing on the Illumina NovaSeq S1 platform for paired-end 150bp reads with 350bp inserts. The sequencing was performed by Novogene where it was arbitrarily sequenced across two lanes, producing two sequencing replicates of vastly different sizes. This is arbitrarily sequencing replicate #1 from that library, and contains the majority of reads.