title: GSM8340254: SUM1315, Hypoxia 24 hr -1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation was performed using the RNeasy Mini Kit. For library construction and subsequent sequencing, 500 ng of total RNA was utilized. RNA library preparations and sequencing were performed using the NEBNext Ultra RNA Library Prep Kit. Libraries were validated on the Agilent TapeStation and quantified with the Qubit 2.0 Fluorometer and qPCR.