title: GSM6762256: WT, liver, rep1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Samples were digested by Proteinase K at 55⁰C overnight and genomic DNA was extracted using the Quick-gDNA MiniPrep kit (Zymo Research). Genomic DNA (100ng) was digested with TaqaI at 65⁰C for 2h followed by MspI at 37⁰C overnight. Following enzymatic digestion, samples were used for library generation using the Ovation RRBS Methyl-Seq System (Tecan). In brief, digested DNA was randomly ligated, and, following fragment end repair, bisulfite converted using the EpiTect Fast DNA Bisulfite Kit (Qiagen). After conversion and clean-up, samples were amplified resuming the Ovation RRBS Methyl-Seq System protocol for library amplification and purification. Libraries were measured using Agilent 2200 TapeStation System and quantified using the KAPA Library Quant Kit ABI Prism qPCR Mix (Roche).