title: GSM7115965: 4C-Seq melanogaster imaginal disc HP6/Umbrea; Drosophila melanogaster; OTHER
library_construction_protocol: imaginal disc tissue is dissected in 1x PBS in 10m intervals, flash frozen. Single nucleus suspension, then double digestion using DpnII and XbaI. proximity ligation, amplification with divergent hp6/umbrea primers with i5/i7 adapters double digest with 4-cutters (dpnii, csp6i), proximity ligation, amplification