title: RNA-seq of Human H1299 Cells: Experiment knock down SNRPG Replicate 3
design: Total RNA was isolated with Trizol (15596026, Invitrogen, Waltham, MA, USA) according to the manufacturers instructions. A total amount of 1 g RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology (Shanghai) Co., Ltd.) following manufacturers recommendations and index codes were added to attribute sequences to each sample.