title: 16S sequencing of mouse gut microbiome: TLR5 Intestine-Specific Overexpression Mice
design: PCR amplification of the V3-V4 region of the 16S rRNA gene was performed on the DNA samples, using primers provided by Shanghai Meiji Biological Company, and constructing the library according to its recommendations. Use the Illumina MiSeq platform for high-throughput sequencing to obtain raw sequencing data.