title: GSM6928079: Zfp352OE,ATAC; Mus musculus; ATAC-seq
library_construction_protocol: mESC pellet was treated transposase at 37°C for 30min ATAC-seq was performed as previously described 69 using MagicSeq Tn5 DNA Library Prep Kit for Illumina (Magic-Bio, M3141). mESC pellet was treated transposase at 37°C for 30min, purified using MinElute PCR Purification Kit (QIAGEN, 28006), and amplified using 1xNEBnext PCR master mix (NEB, M0541S) using custom Nextera PCR primers 1 and 2. Upon purification with MinElute PCR Purification Kit (QIAGEN, 28006). Libraries were subjected to Nova pair-end 150bp sequencing.