title: Amblyomma americanum unfed replicate 1
design: Intact midguts (MG) from unfed Amblyomma americanum adult females were collected and stored in RNAlater solution. The average weight of the group unfed is 4.7 mg. RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.