title: GSM7868714: L363_EV_Ctrl2; Homo sapiens; ATAC-seq
library_construction_protocol: Nuclei from 50,000 cells were isolated by spinning at 500g for 10 minutes, all supernatant was pipet decanted, and pelleted cells were resuspended in 25 uL Tagmentation Reaction Mix composed of 12.5 uL Tagment DNA Buffer (Illumina), 0.02% Digitonin, 0.1% Tween-20, 2.5 uL TN5 enzyme (Illumina). Tagmentation was performed for 1 hour at 37C. Tagmented DNA was isolated by adding Tagmentation Clean-up Buffer (326 mM NaCl, 109 mM EDTA, 0.63% SDS, 20 ug Proteinase K) and incubating at 40C for 1 hour and both a negative (0.7x) and positive (1.2x) SPRI bead (Kapa) selection. Libraries were amplified 12 times with Hifi polymerase (Kapa) and Nextera barcoded primers (Illumina) prior to 1x SPRI bead clean-up and sequencing on a Novaseq (Illumina).