title: GSM7115645: MEF-siControl_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNAs were extracted from 5x105 MEFs, triplicates each experimental group, using Direct-zol RNA miniprep plus kit (Cat#R2073, Zymo Research). 1.5 μl of ERCC ExFold RNA spike-in mix 1 (1: 10 dilution, Cat#4456739, ThermoFisher Scientific Inc.) was added to each siControl RNA sample and 1.5 μl of mix 2 (1:10 dilution) was added to each siHsf1 RNA sample. Sequencing libraries were prepared using Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit.