title: mRNA mock-inoculated tomato leaf 24 hpi
design: Poly(A)+ RNA was isolated from total RNA and fragmented with ultrasound. First-strand cDNA synthesis was primed with a N6 randomized primer. Illumina TruSeq adapters were ligated to the 5' and 3' ends of the cDNA. The cDNA was amplified using a proof reading enzyme, purified using AMPure XP kit and sequenced on an Illumina NextSeq500 platform.