title: GSM7796642: Col0_DAS44_rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was extracted from approximately 100 mg of rosette tissue using the SIGMA Spectrum Plant total RNA KIT (1003037777) according to the manufacturer's instructions with on-column DNase treatment. RNA concentration, quality, and purity was determined using a Bioanalyzer Agilent 2100. All the samples had RIN value between 6.50 and 8.50. BGI prepared the libraries and performed the sequencing. The ribosomal RNA (rRNA) pool was depleted by polyA enrichment. Finally, the strand specific libraries were sequenced with DNBseq platform (paired-end 150 bp reads with a coverage of 40 M reads per library) DNBSEQ Eukaryotic Strand-specific mRNA library