title: cfDNA WGBS
design: Library construction was performed on 25 ng of cfDNA using the Hyper Prep Kit (Kapa Biosystems) with NEXTFlex Bisulfite-Seq Barcodes (Bioo Scientific) and methylated adapters (IDT) along with HiFi Uracil+ polymerase (Kapa Biosystems) for library amplification. NEXTFlex Bisulfite-Seq Barcodes were used at a final concentration of 7.5 uM and the EZ-96 DNA Methylation-Lightning MagPrep kit (Zymo Research) was used for bisulfite conversion of the adapter-ligated cfDNA prior to library amplification. Libraries were sequenced using paired-end 100bp in the platform of HiSeq2500 (Illumina) with a 20% spike of PhiX.