title: GSM8618804: VR23_+WR-1; Homo sapiens; OTHER
library_construction_protocol: DNA was isolated using the DNeasy Blood & Tissue Kit (Qiagen). We amplified the barcodes from the CloneTracker XP™ library and added the Illumina adaptors as well as unique sample index sequences for multiplex sequencing using the NGS Prep Kit for Barcode libraries in pScribe (LNGS-300). After quantification by Fragment Analyzer High Sensitivity NGS kit (Agilent Technologies, Santa Clara, CA, USA) we first combined PCR products from each sample at the same amount, then we purified and concentrated the pool using QIAGEN QIAquick PCR Purification Kit (QIAGEN) following the manufacturer's protocol. We quantified the libraries pool using NanoDrop spectrophotometer