title: RNA-Seq of turbot
design: RNA was isolated using the Maxwell RSC simplyRNA Tissue Kit (Promega) with an automated Maxwell RSC 48 Instrument following the guidelines outlined by the manufacturer. Double-stranded cDNA libraries were constructed using the TruSeq Stranded mRNA LT Sample kit (Illumina, San Diego, CA, USA). Paired-end 150 bp (PE150) sequencing was carried out on an Illumina NovaSeq 6000 sequencer. Both library preparation and sequencing were performed at Macrogen, Inc. (Seoul, Republic of Korea).