title: GSM8126210: RNA-seq Normoxia 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cell pellets collapsed from the detached cells in the ATAC-seq procedure were washed with 1× iced cold PBS and lysed with 1 ml Trizol (Ambion) at room temperature for 5 minutes. To the lysed sample, 200 µl of chloroform was added, and it was then mixed vigorously using a vortex. The mixture was placed on ice for 10 minutes. Following this, samples were centrifuged at 12,000 g at 4°C for 15 minutes. The supernatants were carefully collected and mixed with equal volumes of ethanol. The mixture was transferred to the column and RNA was extracted followed by RNA clean & concentrator-5 (Zymo) with DNase I treatment. The RNA-seq library was constructed using the RiboMinus with stranded RNA hyper prep kit (Zymo) with an input of 1 µg RNA. Sequencing data were generated by Illumina Nextseq 550 with 150 bp paired-end sequence.