title: GSM8170400: EndoC-BH1 Hi-C replicate A, technical replicate 1; Homo sapiens; Hi-C
library_construction_protocol: Hi-C library preparation on two replicates of Endo-C BH1 cells was performed using the Arima-HiC kit according to the manufacturer's protocols. Briefly, cells were crosslinked using formaldehyde. Crosslinked cells were then subject to the Arima-HiC protocol, which utilizes multiple restriction enzymes (targeting ^GATC and G^ANTC) to digest chromatin. Arima-HiC sequencing libraries were prepared by first shearing purified proximally-ligated DNA and then size-selecting 200–600 bp DNA fragments using AmpureXP beads. The size-selected fragments were then enriched using enrichment beads (provided in the Arima-HiC kit). The resulting fragments were converted into Illumina-compatible sequencing libraries with the Swift Accel-NGS 2S Plus DNA Library Kit and Swift 2S Indexing Kit. The purified, PCR-amplified DNA underwent standard QC (qPCR, Bioanalyzer, and KAPA Library Quantification) and was sequenced with unique single indexes on the Illumina NovaSeq 6000 Sequencing System at 2x101 base pairs.