title: GSM8434923: RPE1, AURKB+INCENP, INPUT, R1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 min at room temp and reaction was stopped by adding glycine to a final 0.125M concentration. After cells were washed and lysed, chromatin was sonicated with a Bioruptur Plus (Diagenode). Libraries were prepared using the ThruPLEX DNA-Seq kit (Takara Bio). Adaptors used in the kit include an index (bar code) specific for each sample/library. Samples were normalized and mixed at 1 nM into the same pool. Libraries were amplified using 100 cycles to obtain 50 bp paired-end reads.