title: Whole genome nanopore sequencing of mouse cerebellum (PromethION)_technical replicate 2
design: High molecular weight genomic DNA was extracted from these cerebella using the QIAGEN MagAttract HMW DNA Kit (QIAGEN, 67563) using a modified protocol available from the Oxford Nanopore Technologies Community website (Oxford Nanopore Technologies, 2022). DNA was sheared to a target length of 8kb using a Covaris g-TUBE (Covaris, 520079) and sequenced on high-duplex R10.4.1HD PromethION flow cells protocol with the Ligation Sequencing Kit v14 (SQK-LSK114). For each sample, one R10.4.1HD flow cell was used.