title: GSM8673356: UACC257_WT_DOX_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA samples were prepared using QIAshredder (Qiagen) and RNeasy Plus Mini Kit (Qiagen) according to the manufacturer's recommendations. MELHO cell line RNA-Seq libraries were prepared from 250 ng of total RNA using the TruSeq Stranded Total RNA Library Prep Gold kit (Illumina, 20020599), according to the manufacturer's instructions (Illumina TruSeq Stranded mRNA Sample Preparation Guide, 15031047 Rev. E, October 2013). UACC257 cell line RNA libraries for RNA-Seq were prepared using the TruSeq Stranded mRNA Library Prep kit following manufacturer's protocol.