title: A Single-Center Retrospective Study on the Molecular Epidemiological Characteristics of different Klebsiella pneumoniae infections in Northern China
design: Sequencing library was generated using Rapid Plus DNA Lib Prep Kit for Illumina (Cat.No.RK20208) following manufacturers standard protocol. Individual index sequences were then tagged to each sample. Briefly, genomic DNA samples were fragmented by sonication to an average size of 350 bp. These inserts were then endpolished, A-tailed, and ligated with the adapters, followed by PCR amplification. PCR products were then purified using AMPure XP system (Beverly, USA). The quality of the library was examined using the Agilent 5400 system (Agilent, USA) and measured by qPCR (1.5 nM). The qualified libraries were pooled and sequenced on Illumina platform to generate paired-end reads with length of 150 bp.