title: GSM8301083: control, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: The fresh samples were cut into small pieces after washing with Hanks balanced salt solution (HBSS). A mixture of hyaluronidase, collagenase I, and DNase I was used to digest the tissues at a constant low rate, and gradient centrifugation followed by using a 40 μm filter to remove tissue debris. Then, the cell suspensions were cleaned with DPBS solution excluding calcium and magnesium ions. The 10× genomics technology platform was used for library construction. For specific experimental procedures, please refer to the descriptions of manufacter. The general procedures are as follows: single-cell suspensions are injected and combined with gel beads containing barcode information  with a mixture of cells and enzymes. Then, it enters the reservoir separated by oil to form GEMs, ensuring that the mRNA in cells is only bound to the captured sequences of one gel bead. Subsequently, the gel beads within the GEMs were dissolved, and the cells were lysed to release mRNA, which was reverse-transcribed to produce the barcoded cDNA for sequencing. After the liquid oil reservoir was disrupted, PCR amplification was performed using cDNA as a template.