title: D. melanogaster WGS
design: Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer recommendations (NEB #E7645) from 100 ng of DNA. The quality of libraries was tested by capillary electrophoresis using QIAxcel Advanced (QIAGEN, Germany) and the median peak lengths of the analyzed libraries was 375 bp. Library preparation and pair-end sequencing of 150 nucleotides of inserts were performed in Biobankcore facility of St. Petersburg State University Research Park using HiSeq2500