title: 16S Endophytic F15WWS2MB Offspring Seeds Replicate2
design: Seed surface was sterilized with ethanol 70% during 2 min, followed by 2 min in bleach 50% with a droplet of Tween-20 and five rinses with sterilized MiliQ water. DNA extraction was performed using 250 mg of seed tissue homogenized by maceration in liquid nitrogen, using DNeasy PowerSoil Pro kit, following manufacturer`s instructions. Then the 16S rDNA V3-V4 region was amplified by PCR using 341F and 806R primers. Samples were sequenced using Illumina Novaseq PE 2x250bp reads.