title: RNA-Seq of Melanotic schwannoma
design: After elution and purification, the double-stranded cDNA underwent terminal repair, base A addition, and sequencing splice treatment. It was then subjected to splice connection and PCR amplification. After the amplified products passed quality inspection, denaturation and single-strand cyclization were carried out, and the cyclization products were inspected. Qualified cyclization products were made by DNB, and the constructed DNB was loaded into the MGI platform sequencer for computer sequencing. The sequencing strategy employed was PE150.