title: GSM8377493: 5-NGS126_S17_L001, embryo, CTRL; Bos taurus; RNA-Seq
library_construction_protocol: The total RNA from pools of bovine blastocyst embryos produced in-vitro and treated with or wothout BHB  were extrated with Tryzol  following the manufacturer's instructions. The total RNA was treated with DNase I (Invitrogen; Carlsbad, CA) to avoid DNA contamination. The RNA library preparation from pools of  bovine blastocyst embryos produced in-vitro and treated (n=3) with or wothout BHB (n=3)  were performed with 0.1- 4 µg the total RNA using Illumina TruSeq Stranded mRNA Sample Prep kit. The libraries were quantified by qPCR using the KAPA Library Quantification kit (Illumina; KAPA Biosystems). The sequencing was performed in 1 lane of Hiseq 2500 V4 (2x100pb). PolyA