title: GSM8377210: RNA-Seq B-cells_Y_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq Poly-A based RNA sequencing libraries were generated as described by us (Sun et al.  Nat Comms 2021;  https://doi.org/10.1038/s41467-021-22863-0.). In brief, a 3-prime biased approach was used to generate sequencing libraries. An 8 bp sample index and a 10 bp unique molecular identifier (UMI) were added during initial poly(A) priming and pooled samples were amplified using a template-switching oligonucleotide. The Illumina P5 (5′