title: Heat stress transcriptome analysis in Euglena agilis
design: Heat stress transcriptome analysis in Euglena agilis
library_construction_protocol: The culture was maintained in 100-mL test tubes containing 50 mL of media with aeration (50 mL/min) and constant illumination (100 μmol photon/m2s) in four biological replicates.  We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation. We cultured E. agilis in mAC media at 23°C for more than 3 days and transferred the cells to a temperature of 35°C and incubated them for 8 and 24 h for mRNA preparation. Total RNA was extracted from the cells cultured at 23°C (control) and 35°C for 8 and 24 h using NucleoSpin RNA (Macherey-Nagel).  Sequencing libraries were constructed using a TruSeq Stranded mRNA Sample Preparation Kit (Illumina, Inc.).