title: GSM8127863: GLICO_320_GOLIM4CRISPRi; Homo sapiens; RNA-Seq
library_construction_protocol: After 1 week of co-culture, GLICOs were dissociated with trypsin and single-cell and GFP+/DAPI- cells were sorted. Cells from both samples were submitted for 10x scRNAseq. Library was performed according to the manufacter's instructions (single cell 3' v3 protocol, 10x Genomics). Sorted GFP+/DAPI- GSCs were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel bead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Ilumina R1 primer sequence, Unique Molecular Identifier (UMI) and the 10x Barcode. The pooled barcoded cDNA  was amplified by PCR and the apropriate sized fragments were selected with SPRIselect reagent for subsequent library construction. During the library construction Ilumina R2 primer sequence, paired-end constructs and a sample index were added.