title: GSM7360841: Sut5-PSC; Mus musculus; RNA-Seq
library_construction_protocol: Total mRNA was freshly extracted from FACS-isolated CSC populations from P7 Ctsk-Cre mice (n=5) using RNeasy Plus Micro Kit (Qiagen, cat. 74034). Total RNA integrity was checked using a 2100 Bioanalyzer (Agilent Technologies). cDNA synthesis and amplification were performed by SMART-Seq v4 ultra-low input RNA kit (Takara Bio, USA) starting with 1 ng of total RNA from each sample. 150 pg of qualified full-length double-strand cDNA was used and processed for Illumina library construction with the Nextera XT DNA Library Preparation Kits (Illumina). Then the normalized cDNA libraries were pooled and sequenced on an Illumina NovaSeq6000 sequencer with pair-end 50 cycles.