title: GSM8434961: Rescued, scRNAseq; Drosophila melanogaster; RNA-Seq
library_construction_protocol: The midgut region of 30 Drosophila intestines per sample was harvested in PBS with 2% BSA on ice. Samples were transferred into a tube containing 100µL trypsin (0.75%) and 100µL elastase (2mg/ml). Samples are incubated at 37C for 15 minutes with gentle pipetting in between. 250uL Schneider's media is added into the tubes and tubes are transferred onto ice. Samples are filtered by using 40um strainer and centrifuged for 1.5 minutes at 1000 rcf at 4C. The supernatant was removed and cells were washed with 400uL PBS. After the second wash, 200uL PBS (0.4% BSA) was added to the pellet, and viability was measured using Trypan blue (viability should be close to 90%). Libraries were constructed by the UTSW sequencing core using standardt 10XGenomics protocols.