title: Here analysed gene expression changes after Traumatic Brain Injury (TBI) and subsequent exposure to ISRIB in the brain of adult Danio rerio. 4 groups are available: control (C), traumatic brain injury (TBI; T), ISRIB injection (I) TBI and ISRIB injection (TI).
design: RNA isolation was performed using the ExtractRNA reagent following the standard protocol for TriZol-mediated extraction. The RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program. Total RNA was used for isolation of poly(A)-fraction using NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's recommendations. RNA was next quantified on Quntus fluorometer and used for library preparation using NEBNext UltraTM Directional RNA Library PrepKit for Illumina with NEBNext Multiplex Oligos for Illumina according to the manufacturer's recommendations. The quality of libraries was confirmed by capillary electrophoresis using the QIAxcel Advanced program. Pair-end sequencing was performed using Illumina HiSeq2500 with a read length of 110 bp.