title: GSM7797828: Donor liver biopsy-20; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the flash-frozen liver tissue samples using the miRNeasy Mini Kit (217004; Qiagen, Hilden, Germany) and stored at -80°C. The libraries were constructed using Illumina Stranded Total RNA Prep with Ribo-Zero (20040525; Illumina, California, United States) RNAseq was performed using Illumina NovaSeq 6000 (Illumina). Sequencing was paired-end (2x100 bp) at a read depth of 50 million reads per sample. Fastq generation was performed upon run completion using the Illumina basespace cloud.