title: Term-seq of Escherichia coli K12 MG1655
design: E. coli wild-type strains were grown in LB media in the presence of kanamycin (25 ug/ml) shaking at 37 C. The cells were collected in the mid-exponential phase. RNA was dephosphorylated using the CIP enzyme and rRNA was depleted using a Microbe-express rRNA depletion kit. Unique 2,3-dideoxy RNA oligonucleotide was ligated to the 3' ends of RNA molecules (AAUGAGACACUGAGAUCCAGUCGAUGAGCUA-ddC).