title: RNA-Seq of homo sapiens: gastric tissue
design: Gastrectomy specimens from formalin fixed and paraffin embedded (FFPE) samples were used to identify tissue sections positive for foci of intramucosal signet ring cells (SRC). All SRC foci were microscopically identified as regions of interest (ROIs) based on morphology. An area of adjacent NEP of similar size was selected from each specimen. Excision of SRC ROIs and NEP tissue was performed using laser capture microdissection (LCM). RNA was extracted using the AllPrep DNA/RNA FFPE Kit (80234, Qiagen) following the manufacturer's instructions. Sample preparation was performed using the NEB NEBnext Ultra Low Input Total RNA with rRNA Depletion kit. cDNA libraries were sequenced on the NovaSeq 6000 platform using paired-end sequencing with 100 bp read lengths.