title: Illumina sequencing reads from axenic cultures of Epithemia clementina
design: Isolation of genomic DNA for Illumina sequencing  DNA was extracted from axenic E. clementina cultures following the QIAGEN DNeasy Plant Pro Kit (69206) protocol. For the lysis step, cell suspension was transferred to the kits tissue-disrupting tubes included along with 100mg 0.5mm autoclaved glass beads added and placed in a bead-beater and shaken for one minute. 300ng of the isolated axenic E. clementina DNA was used as input to the NEBNext Ultra II FS DNA Library Kit for Illumina (E7805S). A fragmentation time of 16min was used for a target insert size of 200-450bp. Samples were indexed with NEBNext Multiplex Oligos for Illumina Dual Index Primers Set 1 (E7600S). DNA concentration of resulting libraries was determined with a Qubit dsDNA Quantification Assay High Sensitivity kit (Q32851). Final libraries were checked for quality and size-range using an Agilent Bioanalyzer High-Sensitivity DNA chip. The final mean insert size was 440bp, with a well-formed size distribution around the mean and minimal adapter dimers. The library was sequenced on an Illumina NextSeq 2000 P3 for 2 x 150bp reads.