title: GSM8128010: RH5_ARV771_6h_RNAseq; Homo sapiens; RNA-Seq
library_construction_protocol: To isolate RNA, RH4 or RH5 cells were washed with PBS, then lysed on ice with RLT using the RNeasy mini kit (Qiagen). PDXs were directly lysed with RLT.  For HiChIP, the Dovetail MNase HiChIP kit (Catalogue #21007) was used. Briefly, cells were fixed with DSG and 37% formaldehyde, treated with MNase, and immunoprecipitated with anti-RNA Pol2 antibody (Santa Cruz Biotechnology, Mouse mAb #sc-47701 X). On-bead bridge incorporation and religation was performed, followed by reversal of crosslinks, proteinase K treatment, and DNA purification. Standard Illumina barcodes were introduced during library preparation.