title: Iso-Seq for gene annotation.
design: Total RNA was isolated according to the instruction of Trizol                 method. The integrity, quality and concentration of RNA were detected by NanoDrop                 2000 spectrophotometer (Thermo Fischer Scientific) and Angilent 2100 Bioanalyzer                 (Agilent Technologies). High quality RNA (2g, 300ng/l) was prepared for the next                 step of library construction.