title: GSM8591230: WT_noTrp_exp2_biol_rep_3; Mus musculus; RNA-Seq
library_construction_protocol: To study transcriptional response in mouse tissues, total RNA was isolated from the homogenates by trizol (1:10 ratio) method. mRNA was purified by NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) from Turbo-DNAse treated total RNA. A NEB Next® Ultra Library Preparation Kit (NEB E7530S) was used to prepare 0.5 µg of total RNA for RNA-seq.