title: GSM6929041: Dbx2_constitutive_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from frozen cell pellets of control and Dbx2-overexpressing non-adherent cultures using Qiagen RNeasy kits Total RNA (100 ng) from each sample was prepared using QuantSeq 3'mRNA-Seq Library prep kit (Lexogen, Vienna, Austria) according to manufacturer's instructions. The amplified fragmented cDNA of 300 bp in size were sequenced in single-end mode using the NextSeq500 (Illumina) with a read length of 101 bp.