title: GSM8171543: 1mm bone defect, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: The regenerative tissues from the defect gap were harvested at 3 weeks post-surgery and dissociated in 2 mg/mL Collagenase P and 2 mg/mL pronase at room temperature for 1 hour. The erythrocytes were removed using anti-GYPA and Streptavidin Microbubbles. Subsequently, the cell suspension was filtered through a 30 μm strainer to yield a single-cell suspension. Two sets of single-cell libraries were prepared: one from cells derived from a 1 mm bone defect (control, n=3) and the other from cells obtained from a 3 mm bone defect (nonunion, n=3). For each sample, ten thousand cells were loaded for processed to generate single-cell mRNA libraries using the Chromium Single Cell 3′ kit (v3.1 Chemistry, 10× Genomics Inc).