title: BGISEQ-500 paired end sequencing; RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations
design: RNA-seq of Arabidopsis thaliana plants treated with putrescine, spermine, flg22 and combinations
library_construction_protocol: Polyamine, flg22 and mock (water) treatments were performed in three biological replicates by leaf- infiltration of 5-week-old wild-type (Col-0) plants. Infiltrated leaves were collected and frozen in liquid nitrogen at 24 h of treatment for total RNA extraction. Seeds of the different genotypes were sown on a soil mixture containing peat moss (40%), vermiculite (50%) and perlite (10%), and stratified in the dark at 4°C for 2-3 days. Plants were grown at 20–22℃ under 12-hour light/ 12-hour dark photoperiod cycles. Leaf-infiltration with 1-ml needless syringe Total RNA was extracted using TriZol (Thermo Fisher) and further purified using RNeasy kit (Qiagen) according to manufacturer's instructions. Libraries were prepared using the MGIEasy RNA Library Prep kit (MGI Tech) according to manufacturer's instructions