title: RNA-Seq of migratory locust:locust brain
design: Brain samples were dissected from migratory locusts with RNH1 knockdown. The TRIzol reagent (Thermo Fisher Scientific, Waltham, USA) was used for RNA extraction. DNase I was used to eliminate DNA contamination after the extraction process. A 2100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, USA) was used to verify the integrity of the RNA samples before sequencing. A TruSeq RNA sample preparation kit (Illumina Inc, SanDiego, USA) with 2 g of RNAs from each sample was used for cDNA library construction, according to the manufacturers recommendations. We used the TruSeq SBS Kit on the HiSeq 2500 platform for pared-end sequencing (2150 bp).