title: RNA-seq of Serritaenia testaceovaginata aka Mesotaenium testaceovaginatum (Zygnematophyceae, Streptophyta) treated with ultraviolet radiation against untreated control
design: RNA-seq of Serritaenia testaceovaginata aka Mesotaenium testaceovaginatum (Zygnematophyceae, Streptophyta) treated with ultraviolet radiation against untreated control
library_construction_protocol: Algal cells were collected by centrifugation (500 g, 5 min), lysed as described in (Gerbracht et al., 2022) Algae were pre-grown in tissue culture flasks in liquid culture medium KW (Table S1) at 30 µmol m-2 s-1 photosynthetically active radiation (PAR) (LinearZ SunLike LEDs, 5700K, Lumitronix, Hechingen) with a 14/10 h light/dark cycle for four weeks. Before the start of the light experiment, the cells were transferred to Petri dishes with fresh medium and acclimatized to higher PAR conditions (120 µmol m-2 s-1, 14/10 h) for ten days.  During the experiment the algae were exposed to the above-mentioned LEDs and the UVB Broadband TL fluorescent tube lamp (20 W, Philips, Hamburg), resulting in 120 µmol m-2 s-1 PAR, 400 µW cm-2 UVB (280-315 nm), and 150 µW cm-2 UVA (315-400 nm). While PAR was applied in the regular 14/10 h photoperiod, UVR was applied for 4 h at noon. Control cells were covered with a Makrolon® polycarbonate plate that blocks UVR < 390 nm (Busch & Hess, 2021). The experiment was run in triplicates for three days at 16 °C. After the third UVR exposure, when the the algae displayed slight extracellular pigmentation, all samples were subjected to RNA isolation. RNA isolation with the TRIzol Reagent (Thermo Fisher Scientific Inc., Waltham) according to the manufacturer's protocol RNA samples were submitted to the Cologne Center for Genomics (CCG) (Cologne, Germany) for paired-end mRNA library preparation (Illumina TruSeq mRNA stranded, Illumina, San Diego)