title: GSM7116135: Hep3B T0; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was isolated using the TIANamp Genomic DNA Kit (#DP304; TIANGEN) as the manufacturer's instructions. PCR was used to amplify sgRNA barcodes (#M0544S; New England Biolabs), and deep sequencing was then performed to assess the sgRNA sequences.