title: GSM8264822: WT, Ad; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: L1 larvae were collected in a starved state following the overnight hatching period. For adult worms, starved L1 were plated on NGM agar with food and grown at 20 degrees C for approximately 68 h Poly(A)+ RNA-seq libraries were prepared from 3 µg total RNA from either late embryos or L1 larvae (for analysis of miR-35–42 target mRNAs) or from 10 µg total RNA from gravid adults (for analysis of protein-coding genes near dysregulated piRNAs on Chromosome IV) using the stranded NEXTFLEX Rapid Directional RNA-seq kit (Perkin Elmer).