title: Transcriptome of Staphylococcus equorum strain BC9 growing on cheese with Penicillium strain 277
design: Total RNA was extracted from biofilms growing on cheese using a phenol-chloroform approach supplemented with lysostaphin. Bacterial and fungal rRNA were depleted with NEBNext rRNA Depletion Kit in tandem with a custom designed pool of complementary Penicillium rRNA sequences. mRNA was then prepped using NEBNext Ultra II RNA Library Prep Kit for Illumina and sent to Tufts University Core Facility Genomics, where it was pooled and sequenced for RNA-seq library construction. Libraries were sequenced on a NextSeq 550.