title: GSM7360636: RPE-1 hTERT FUCCI H3K9me3 rep 4; Homo sapiens; OTHER
library_construction_protocol: TrypLE digestion for RPE-1 hTERT FUCCI cells Custom library protocol. Briefly, cells were incubated with the antibodies listed above and a pA-MNase fusion protein. Next, individual cells were sorted into 384-well plate. Subsequently, MNase digestion was initiated through the addition of calcium. Next, MNase digestion was stopped and DNA released by Proteinase K digestion. DNA fragments were end-repaired by Klenow Large Fragment and PNK. Repaired ends were ligated to custom barcoded adapters containing a UMI, Illumina handle and T7 promoter. After IVT and PCR, sequencing libraries were assessed with Qubit and Bioanalyzer and normalized libraries loaded onto Illumina sequencer according to the manufacturers instructions. Illumina RPI small RNA adapters