title: GSM8442784: in vitro cells, Tgfb treated, day 12; Mus musculus; RNA-Seq
library_construction_protocol: Both microdissected limb paddes and high density cell cultures were dissociated using collagenase supplemented with DNAse. We confirmed that the viability of the cells post dissociation was greater than 80%. Cells were counted and loaded onto the 10X chip following the 10x v3.1 protocol RNA libraries were prepared using the 10x Chromium v2 3' kit following the manufacturer's protocols