title: RNA-Seq of Wild Type Acropora digitifera: Light Dark Conditions at 6am colony B
design: One and a half g RNA from each sample (three biological replicates from each sampling point n=39) was sent for sequencing. RNA samples were prepared using the Illumina TruSeq RNA Library Preparation Kit v2, according to the manufacturers protocol. Libraries from each sampling point were run on lanes of an Illumina NextSeq machine using the multiplexing strategy of the TruSeq V2.5 protocol. The protocol starts with poly-A selection that results in RNA selection only. Single-end reads were obtained for each sample; the reads were 75 bases long. All sequencing libraries were trimmed using TrimeGalore version 0.4.0 (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/) to remove adapters, primers, and low-quality bases.