title: scRNA-seq of CAR-1
design: Tumor tissues were transported on ice in DMEM/F-12 medium immediately after surgical resection. The tumor was minced into smaller fragments and dissociated into single-cell suspensions. The single-cell suspensions were then loaded into the HIVE CLX system (Honeycomb), and libraries were prepared using the HIVE system and sequenced by Honeycomb. Raw Illumina sequencing reads were aligned to the GRCm38 (mm10) reference genome (Mus musculus) using the BeeNetPLUS (Honeycomb) with default parameters. Subsequently, genes were quantified as UMI counts, and count matrices, aligned BAM, and QC files were generated using the BeeNetPLUS workflow on Terra.bio (Honeycomb).