title: metagenomic sequencing of a microbial community grown on ethylene glycol
design: At the end of the experiment, two 10 mL subsamples were collected from each culture, centrifuged at 10,000 x g for 10 minutes, and decanted. The resulting cell pellets were used for DNA and RNA extractions using MP Biomedicals extraction kits according to the manufacturer instructions. Illumina metagenomic sequencing was performed at the University of Utah Huntsman Cancer Institute High-throughput Genomics Facility. Metagenomics libraries were produced from extracted DNA using the Illumina Nextera DNA Flex Library Prep. Libraries were sequenced on the Illumina NovaSeq using a paired end 2 x 150 bp kit. Metatranscriptomic sequencing was performed at the Microbial Genome Sequencing Center (MiGS)