title: GSM8217623: CHD6_CUT&Tag_HEK293_rep3; Homo sapiens; OTHER
library_construction_protocol: Harvest HEK293 cells were grown with indicated treatments and count the cells.  Cells should be intact and in a homogenous, single-cell suspension.  Immunoprecipitated using indicated antibodies, transposition reaction and purification DNA fragments and amplify transposed DNA fragments by PCR. DNA libraries were prepared according to NovoNGS CUT&Tag 4.0 High-Sensitivity Kit for Illumina and sequenced on an Illumina NovaSeq6000 platform to generate 150 bp paired-end reads.