title: DNA-seq of deep seafloor sediment: Sediment1cm_BHT
design: The metagenomic sequence libraries were constructed using the Illumina DNA Prep (M) Tagmentation Kit with Nextera DNA CD Indexes for barcoding according to the manufacturers instructions. The quality of the constructed DNA libraries was evaluated using an Agilent 2100 Bioanalyzer with a High Sensitivity DNA Kit according to the manufacturers instructions. Paired-end sequencing (150 bp  2) was performed using the HiSeq X platform.