title: GSM8442989: HCT16T61AKO1 MeRIP; Homo sapiens; OTHER
library_construction_protocol: RNA fragments are first ligated at 3' end before the chemical reactions. Briefly, the process involves the following steps: They, whether undergoing biotin pulldown or not, are then subject to reverse transcription before 3' adaptor ligation to the resulting cDNA. Only those fragments with internal m1A sites are expected to generate misincorporation during reverse transcription that can be further detected using high-throughput sequencing.