title: GSM8441363: HC_21_Tconv_Exvivo; Homo sapiens; ATAC-seq
library_construction_protocol: na Cells were pelleted by centrifugation at 500 x g for 7 min at 4°C, then resuspended with 50 μl of transposase mixture (25 μL of 2x TD buffer (Illumina), 2.5 μL of TDE1 (Illumina), 0.5 μL of 1% digitonin (Thermo Fisher), 22 μL of nuclease-free water). Transposition reactions were incubated at 37°C for 30 minutes in a thermal shaker with agitation at 300 RPM. Transposed DNA was purified using a MinElute Reaction Cleanup kit (QIAgen) and purified DNA was eluted in 20 μL elution buffer. Transposed fragments were amplified and purified as described previously with modified primers. Libraries were quantified using qPCR (KAPA Library Quantification Kit) prior to sequencing. All Fast-ATAC libraries were sequenced using a 2x100 bp paired-end protocol on the HiSeq 4000 Sequencing System (Illumina).