title: GSM7114940: WT_H3K27Me3_Rep2; Mus musculus; ChIP-Seq
library_construction_protocol: MEFs were fixed in 1% formaldehyde in PBS for 20 min and quenched with 125 mM glycine for 5 min at room temperature. Sonicated chromatin extract was immunoprecipitated overnight with corresponding antibodies. Protein A sepharose was used to capture immune complexes. Immune complexes were washed, de-crosslinked and DNA was extracted. ChIP-seq libraries were prepared using the ThruPLEX® DNA-seq Kit V2 (Rubicon Genomics, Ann Arbor, MI)