title: ITSrRNA:pea rhizosphere
design: At the pea vegetative stage, rhizosphere soil samples from three plants in each treatment per plot were collected for DNA extraction. Genomic DNA of rhizosphere soil fraction was extracted using a PowerSoil kit (Qiagen, Valencia, CA, USA) . ITS1 region  was amplified using ITS1F/ITS2_Nextera primer pairs (White et al. 1990; Gohl et al. 2016). The PCR product was diluted 1:100 with molecular-grade water. The diluted amplicons were indexed using barcoded PCR primers (Gohl, et al., 2016). Barcoded amplicons were normalized using SequalPrep kits (Invitrogen).