title: 6-OHDA-treated,scRNA-seq
design: For single-cell sequencing of mouse AOM/DSS colorectal tumors in this study, the single-cell sequencing tissues were obtained from 5 control-treated and 5 6-OHDA-treated mice with colorectal cancer mice.Tumor tissues from each group were pooled together and digested for tissue dissociation. After blocking cell surface antigens with anti-mouse CD16/CD32, epithelial cells and immune cells in the tumor were labeled using FITC-labeled EpCAM and FITC-labeled CD45, respectively. The viability of tumor cells was assessed using 7-AAD to distinguish live and dead cells. Lived EpCAM+ and CD45+ cells were sorted using FACS  for single-cell sequencing.