title: GSM8217937: M82 H3K4me3 Rep1; Solanum lycopersicum; ChIP-Seq
library_construction_protocol: ChIP-seq assays were performed on 4-week-old tomato fourth branch of leaves according to Bio-protocol of Ramirez-Prado, J.S. et al (2021) by using anti-H3K4me3 (Millipore 07-473), anti-H3K36me3 (Abcam. ab9050) antibodies. ChIP-seq libraries were prepared from 10ng of DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to manufacturer's instructions. One biological replicate was generated for each condition. DNA libraries were checked for quality and quantified using an Agilent 2100 Bioanalyzer (Agilent) and subjected to 1 × 75 bp high-throughput sequencing by NextSeq 500 (Illumina).