title: GSM8619366: MASH, replicate4, snATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: Frozen human liver tissue was homogenized using a Dounce homogenizer in lysis dilution buffer. The homogenate was centrifuged, and the pellet was resuspended in lysis dilution buffer. The nuclei were washed and resuspended in lysis dilution buffer. Nuclei were further purified using the Nuclei Isolation Kit (Sigma, Cat# NUC201) with a 1.8 M sucrose cushion, following the manufacturer's instructions. After centrifugation at 30,000 g (approximately 13,000 rpm) for 45 minutes at 4 °C, the nuclei pellet was collected and resuspended in PBS containing 1% BSA. The nuclei were filtered through a 40 μm cell strainer before loading onto the 10x Genomics Chromium Controller. snATAC-seq libraries were prepared according to the manufacturer's instructions of 10XGenomics (PN-1000176)