title: RS-1-2
design: fungal primers ITS1F and ITS2R [Walters et al., 2015]. Each primer set also contained the Fluidigm adaptor primers CS1F and CS2R [Green et al. 2015].(PCR setting: 25 cycles of 10 sec at 95C, followed by 1 sec at 50C (for 16S) and 52C (for ITS), followed by 1 sec at 68C).Amplicon sequencing was conducted using a paired end 2x250 bp cycle run on Illumina MiSeqV2 chemistry at Rush University Genomics and Microbiome Vore Facility