title: GSM8264304: S. rosetta cell-type transcriptomes:  Thecate, replicate 1; Salpingoeca rosetta; RNA-Seq
library_construction_protocol: 10 million cells were lysed in 100 µl of preferential lysis buffer ( 20 mM Tris-HCl, pH 8.0; 150 mM KCl; 5 mM MgCl2; 250 mM sucrose; 1 mM DTT; 10 mM digitonin; 1 mg/ml sodium heparin; 1 mM Pefabloc SC; 100 mg/ml cycloheximide; 0.5 U/ml Turbo DNase; 1 U/ml SUPERaseIN). This was incubated on ice for 10 minutes, passed ten times through a 30G needle and centrifuged at 6,000 x g for 10 minutes at 4C. The supernatant was collected, brought to 100 µl with RNAse-free water, and RNA was purified using the RNAeasy kit from Qiagen (Cat. No. 74104), and eluted in 30 µl of water. KAPA mRNA HyperPrep kit (KAPA biosystems, Cat. No. KK8580) according to manufacturer's instructions