title: ChIPSeq of npc
design: ChIPSeq libraries for BRN2 were prepared using NPCs from 3 ASD lines and 3 control lines. About 10 million NPCs for each library were collected between passages 6-9 at day 3 (about 80% confluency) and cross linked using 4% formaldehyde for 10 mins at room temperature. The cells were resuspended in lysis buffer, sonicated, and incubated with antibody (POU3F2 (D2C1L) from Cell Signaling) linked DynaBeads Protein G overnight at 4 C.