title: WGS of Clonostachys rosea: mycelia grown on PDA
design: The strain of C. rosea utilized in our study was provided by the Scion Research Institute in Rotorua, New Zealand. To isolate DNA from C. rosea, we used a PowerSoil DNA Isolation Kit (MoBio Laboratories, Carlsbad, CA, USA). First, the fungal strain was grown on Potato Dextrose Agar (PDA) plates at 25C for five days, and then approximately 200 mg of mycelium was scraped off the plate using a sterile loop and transferred to a 2 mL PowerBead tube. We then followed the manufacturers instructions for DNA extraction, except for the mechanical lysis step, which was performed using a TissueLyzer II (Qiagen, Valencia, CA, USA) for 2 min at 30 Hz. After extraction, the genomic DNA was cleaned and concentrated using a DNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA, USA), following the manufacturers instructions and the pellet was resuspended in UltraPure DNase/Rnase-Free Distilled Water (20 l; Cat No. 10977015; Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). The quality and quantity of the extracted genomic DNA were initially assessed using a Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA, USA) and agarose gel electrophoresis to ensure the DNA was of high integrity and of sufficient concentration for DNA sequencing.  Genomic DNA was stored for downstream applications at -80 C. Whole genome sequencing was performed by Auckland Genomics Ltd.  (University of Auckland, NZ). Auckland Genomics performed library preparation and sequenced the sample DNA on an Illumina MiSeq platform using a 500-cycle MiSeq Nano 2 x 250 bp kit, as per the manufacturers protocols.