title: GSM8170496: WT_SteadyState_GEX_rep2; Mus musculus; RNA-Seq
library_construction_protocol: For all scRNA-seq experiments, samples were centrifuged (950g for 5 min) and then pellets were resuspended in 1x PBS plus 0.04% BSA (Amresco) at a concentration of 1x106 cells per mL. Single-cell suspensions were loaded onto a Chromium Controller (10x Genomics) to generate 10,000 single-cell gel beads in emulsion per sample, and each sample was loaded into a separate channel. The cDNA was amplified (11 cycles) and either used for TCR enrichment/library preparation with the Chromium Single Cell V(D)J TCR kit or for preparation of a gene expression library. For scRNA-seq, libraries were prepared using the Chromium Next GEM 5' v2 (dual index) and Gel Bead kit (10x Genomics).