title: GSM8498586: RPE1-hTERT TP53-/- PACKO Cas9 ZRANB3-/- T18_A; Homo sapiens; OTHER
library_construction_protocol: DNA was harvested by QIAamp Blood Maxi Kit (QIAGEN) Genome-integrated sgRNA sequences were amplified by PCR using Q5 Mastermix (NEB Next UltraII, New Endland Biolabs, M0544S). i5 and i7 multiplexing barcodes were introduced in a second round of PCR CRISPR screens