title: RNA-Seq of human ocular fibroblasts: TGF-beta1+artesunate treated
design: After the sample is qualified, magnetic beads with Oligo (dT) are used to enrich eukaryotic mRNA. Then add fragmentation buffer. The mRNA is randomly fragmented. Using mRNA as a template, six-base random primers (random hexamers) are used to synthesize cDNA. First strand, then add buffer, dNTPs and DNA polymerase I to synthesize the second strand of cDNA, and then use AMPure XP beads purification of double-stranded cDNA. The purified double-stranded cDNA was then end-repaired, A-tailed, and connected to sequencing adapters, and then used with AMPure XP beads fragment size selection was performed, and finally PCR enrichment was performed to obtain the final cDNA library.