title: Metagenome
design: Genomic DNA was extracted from an 8 m filter following the filtration of 2 mL concentrated UC22 protist culture. The ZymoBIOMICS DNA Miniprep Kit was used for extraction, and DNA quantity was determined with a Qubit HS dsDNA assay (Invitrogen). A library was prepared using the Native Barcoding Kit 24 V14 according to the manufacturers protocol and sequenced on MinION flowcells, R10.4.1.