title: GSM6883139: 4_Ventricular tissue, P23. biol rep 01; Mus musculus; RNA-Seq
library_construction_protocol: Heart (ventricular) tissue samples were homogenized in TriZol regaent. Total RNA was extracted using chloroform-isopropanol protocol. Isolated total RNA was purified using DNA-free kit (Ambio) according to manufacturer's instructions. RNA quality and integrity was analyzed using NanoDrop 2000 spectrophotometer and Agilent 4200 Tapestation system. The smRNA-sequencing libraries were constructed by using SMARTer smRNA-Seq kit for Illumina (Takara Bio) following manufacturer's recommended standard protocol.