title: GSM6670822: M5215-1; Mus musculus; RNA-Seq
library_construction_protocol: Mice PBMCs and spleen single cell suspension were extracted, and the dead cells were removed before sequencing Cells from the spleen and PBMCs of infected (3 mice pooled) and uninfected (3 mice pooled) mice were washed once in PBS with 0.04% BSA and loaded to the 10X Genomics Chromium to capture single cells for scRNA-seq following the manufacturer's instructions of 10X Genomics Chromium Single-Cell 5' kit (V2.0). In brief, gel beads containing barcode information were combined with a mixture of cells and enzymes and then encapsulated by droplets of oil surfactant in a microfluidic "double-cross" system to form gel beads-in-emulsions (GEMs), which flowed into a reservoir and were collected. The gel beads were lysed to release the barcode sequence, cDNA fragments were reversed transcribed, and the sample was labeled. After breaking the gel beads and oil droplets, cDNA was broken into fragments of about 200-300 bp using enzymes, and sequencing connectors and primers were added. Finally, polymerase chain reaction (PCR) amplification was performed to obtain the DNA library. Libraries were sequenced on an Illumina NovaSeq 6000 (LC-Bio Technology Co. Ltd., HangZhou, China) sequencing system using a 150 bp paired-end multiplexing run.