title: Illumina iSeq 100 paired end sequencing of SAMD00521519
library_construction_protocol: "Fish eDNA was amplified using MiFish primers (Miya et al. 2015) and subsequently indexed for illumina paired end sequencing. The PCR products were pooled and then purified with AMPure XP (BeckmanCoulter). The target size amplicon (approximately 370 bp) was excized using 2% E-Gel SizeSelect (ThermoFisher Scientific) and quantified using a Qubit dsDNA HS assay kit and Qubit 4 Fluorometer (ThermoFisher Scientific). Its purity was evaluated using Qsep1 Bio-Fragment Analyzer (BiOptic) or Agilent 2100 BioAnalyzer (Agilent). The library diluted to 40 pM with 10 mM Tris-HCl buffer (pH 8.5) or Buffer EB (Qiagen) was sequenced with an iSeq 100 (illumina, Inc.), using iSeq 100 i1 Reagent v2 with 30% PhiX (illumina, Inc.) spike-in for quality control."