title: GSM8128039: panicles, OsM2E_WT1; Oryza sativa; RNA-Seq
library_construction_protocol: First, total RNA was extracted from panicle tissue using the TRIzol R  reagent (Invitrogen, Carlsbad, CA, USA). RNA was then treated with DNAse I (M0303S  NEB, Massachusetts, USA) to remove contaminating gDNA according to the manufacturer's  protocol. RNA quality and concentration were examined on a 4150 TapeStation (Catalog:  G2992AA, Agilent Technologies, California, USA) and Qubit (Life Technologies, California,  USA), respectively. RNA of integrity number (RIN) > 7 was used for library construction.  Total RNA of 500 ng was used to enrich mRNA using the NEBNext Poly (A) mRNA magnetic isolation module (Catalog: E7490, New England Biolabs) following the  manufacturer's protocol. Enriched mRNAs were used for library preparation using the  NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England  Biolabs). Sequence data was generated using the Illumina HiSeq, pair-end 2X150bp chemistry.