title: GSM8171418: Photoreceptor nuclei, dsRNA targets at Trx, ZT8, rep3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 400 flies are decapitated by vortexing after being flash frozen in liquid nitrogen. Fly heads are separated using pre-chilled sieves of different sizes. The fly heads are then homogenized using glass homogenizer and the homogenate (cell debris and intact nuclei) are incubated with Anti-GFP-attached beads to enrich GFP-tagged photoreceptor nuclei. Photoreceptor nuclear RNA are extracted using ZYMO Direct-zol RNA Microprep Kits (#R2061). cRNA library is prepared using Ovation® SoLo RNA-Seq System (#0502-32) that has Drosophila ribosomal RNA depletion built into the protocol.