title: GSM8377761: 200nM Aphidicolin treated hIPSC labeled with double pulse of EdU plate 3; Homo sapiens; OTHER
library_construction_protocol: cells were sorted into 384 well plates and protK treated prior to library construction See van den Berg et al.,(scEdU-seq, Nature Methods, 2024) scEdU-seq library strategy was used. Briefly, cells were lysed with protein digestion. Subsequently, DNA was fragemented with NlaIII and  adapters were ligation followed by amplification with IVT and PCR