title: GSM8673467: DMNQ_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: 100.000 cells were washed twice in 1 mL ice-cold PBS, by centrifugation at 500 g for 5 min. Pellets were resupended in 100 µL ice-cold ATAC Lysis Buffer and processed with the ATAC.seq kit (Cat # 53150; Active Motif) according to manufacturer's instructions. 33.5 µl tagmented DNA were mixed with 2.5 µl I7 index primer, 2.5 µl 2.5 uM I5 index primer (dual indexing), 1 µl dNTP, 10 µl 5x Q5 Reaction buffer and 0.5 µl Q5 Polymerase and processed accordingly to manufacturer's instructions.