title: DNA-seq of 5% mutation:KRAS 12C+13D
design: Firstly, the sequencing in this experiment was performed using a Qcell-3841 flow cell on a QNome-3841 hex (QitanTech, China).However, the required fields "platform" and "instrument_model" in this table do not include the sequencing instrument options we used, so we can only select the OXFORD_NANOPORE option, which is similar as QNome-3841 hex. It is important to note that we did not use OXFORD_NANOPORE for sequencing; the sequencing platform we used is QNome-3841 hex (QitanTech, China). We are only filling in the existing sequencing platforms in the table due to its limitations. Therefore, we hope that the SRA staff can manually modify the sequencing platform information to the correct details upon receiving our form. The correct information is: the "platform" column should all be "QitanTech"; the "instrument_model" column should all be "QNome-3841 hex." Secondly, the library construction strategy we used is based on designing primers with specific adapter sequences on both ends of the PCR products, followed by rolling circle amplification.