title: GSM8217935: Kyp ATACseqRep1; Solanum lycopersicum; ATAC-seq
library_construction_protocol: Approximately 100 mg of 4-weeks-old tomato fourth leaves were ground, passed through a 100 µm filter and the nuclei were isolated using nuclei isolation buffer (0.25M sucrose, 10mM Tris-HCl pH8, 10mM MgCl2, 1% Triton X-100, 5mM 2-mercapto-ethanol, 0.1 mM  protease inhibitors). The nuclei were re-suspended in 2× TD buffer (20 mM Tris-HCl pH 7.6, 10 mM MgCl2, Dimethyl Formamide 20%) and 2.5 µl of Tn5 transposase (Illumina FC-121-1030) were added. Transposition reaction was performed at 37 °C for 30 min, and DNA was purified using a Qiagen MinElute Kit (QIAGEN, Cat.No.28004). DNA libraries were amplified for a total of 10 cycles as described by Buenrostro et al. (Nat Methods.2013) and Jegu et al. (Genome Biol. 2017). DNA libraries were checked for quality and quantified using a 2100 Bioanalyzer (Agilent) and subjected to 2 × 75 bp high-throughput sequencing by NextSeq 500 (Illumina).