title: GSM7469222: ATP7B_m_noCu_1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted after a growth of three days on a NGM  agar plate for each condition Total RNA was quantified using the Qubit 4.0 fluorimetric Assay (Thermo Fisher Scientific). Libraries were prepared from 125 ng of total RNA using the NEGEDIA Digital mRNA-seq research grade sequencing service (Next Generation Diagnostic srl; https://doi.org/10.1038/s41598-017-14892-x) which included library preparation, quality assessment and sequencing on a NovaSeq 6000 sequencing system using a single-end, 100 cycle strategy (Illumina Inc.). NEGEDIA Digital mRNA-seq