title: RNA-Seq of rattus norvegicus: adult male left ventricle
design: First-strand cDNA was generated using random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis.The synthesized cDNA was subjected to end-repair and then was 3 adenylated. Adapters were ligated to the ends of these 3 adenylated cDNA fragments.This process was to amplify the cDNA fragments with adapters from previous step. PCR products were purified with Ampure XP Beads (AGENCOURT), and dissolved in EB solution.Library was validated on the Agilent Technologies 2100 bioanalyzer.The double stranded PCR products were heat denatured and circularized by the splint oligo sequence. The single strand circle DNA (ssCir DNA) were formatted as the final library.