title: WGS of Thlaspi densiflorum
design: Before NGS library constructions, three qualified DNA samples (three replicates) were fragmented into short-insert 400-500 bp fragments, then fragment size distributions were checked using the QIAxcel Advanced System (Qiagen) and quantified using the Qubit dsDNA HS Assay Kit (Life Technologies). Short-insert libraries of 500 bp fragments were constructed using the Illumina Nextera DNA Library Prep Kit instructions (Illumina). All libraries were sequenced on the Illumina NovaSeq 6000 platform to generate paired-end reads of 150 bp at Genoks LifeScience Sequencing Center, Ankara, Turkey.