title: Illumina MiSeq microbiome of Ramalina gomerana
design: Genomic DNA was extracted using the PowerSoil DNA Isolation Kit following the instructions of the manufacturer. The V1V2 hyper-variable region of the bacterial 16S rRNA was used as barcode to prospect microbial diversity. We used universal primers 27F and 338R for amplification. PCR products were checked in 1% agarose gels stained with SYBR Safe DNA Gel Stain and quantified using the Qubit dsDNA HS (High Sensitivity) Assay Kit. Then they were pooled in equimolar concentrations and sequenced on a single MiSeq run (Illumina, USA) using v2 chemistry and 2  250-bp paired-end reads at the RTSF Genomics Core at Michigan State University.