title: GSM8218591: 8dpf larvae, wt-1; Danio rerio; RNA-Seq
library_construction_protocol: Wt and grwd1 crispants at 8dpf  were euthanized by immersion in ice water for 10 minutes and then were collected for RNA extraction. N=15 larvae were pooled for each group. RNA were extracted using RNAeasy mini kit(Qiagen). 600ng of total RNA were used for the construction of sequencing libraries. RNA library preparation follows the Illumina® Stranded Total RNA Prep with Ribo-Zero Plus protocol to generate polyA-enriched, rRNA-depleted libraries. Illumina NovaSeq - S4 flowcell