title: GSM8434482: Jurkat-J2, H3K27Ac, ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde solution and quenched with 120 mM Glycine. Samples were then treated with 0.5% Igepal supplemented with 1 mM PMSF, pelleted, snap frozen, and sent to Active Motif (Carlsbad, CA, USA) for H3K27Ac-ChIP-seq analysis. Lysates were sonicated, and the DNA sheared to an average length of 300–500 bp with an EpiShear probe sonicator (Active Motif, cat# 53051). Illumina sequencing libraries were prepared from ChIPs and Input DNAs at Active Motif  on an automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on the Illumina NextSeq 500 platform.