title: GSM8217731: Mouse colon, ChronicColitis1; Mus musculus; RNA-Seq
library_construction_protocol: Intestinal tissues were prepared from C57BL/6 female mice after induction of the acute or chronic colitis model and homogenized for scRNA-seq analysis. We used Chromium Next GEM Single Cell 3p RNA library v3.1. After dissociation, single cells, reagents, and a single Gel Bead containing barcoded oligonucleotides were encapsulated into nanoliter-scale GEMs (Gel Bead in emulsion) using the Next GEM Technology. We then sampled a pool of about 3,500,000 10x Barcodes to separately index each cell's transcriptome and cell surface protein by partitioning thousands of cells into nanoliter-scale Gel Beads-in-emulsion (GEMs), where all generated DNA molecules share a common 10x Barcode. - The poly(dT) primer captures poly-adenylated mRNA and barcoded, full-length cDNA is produced. Single Cell 3' GEX and feature barcode libraries were sequenced on the Illumina sequencing system. For paired-end sequencing, two reads were sequenced from both ends of the fragment. Library preparation and sequencing were conducted by Macrogen (Seoul, Korea).