title: WGS of CWBI, population LT-04, 15 transfers, clone 01
design: For Illumina sequencing, genomic DNA was isolated from 1 mL of culture using a PureLink Genomic DNA Mini Kit (Invitrogen). A Qubit 2.0 spectrophotometer with the dsDNA Broad Range Quantitation kit (Invitrogen) was used to measure DNA concentrations. Up to 10 ng of genomic DNA was used as input to the IDT DNA Lib Prep EZ using the xGen Deceleration Module according to the manufacturers instructions except that all reactions used 20% reaction volumes. Unique combinations of six-base dual barcodes were used with each sample. Libraries were pooled and sequenced to obtain paired-end 150-base reads.