title: GSM8673388: CnT_UACC257_WT_DOX_rep2; Homo sapiens; OTHER
library_construction_protocol: Protocol as in "Barbosa et al., Nat Commun. 2023 Jul 3;14(1):3907": 400,000 cells were harvested, washed and mixed with concanavalin A-coated magnetic beads; Cells were suspended in a buffer containing the primary anti-HA antibody (overnight at 4 °C), followed by secondary antibody (1 hour, room temperature). After addition of the adapter complex, washing steps and reaction termination, DNA was extracted and PCR reactions were performed with indexing primers to generate the library.