title: GBS of Pinus radiata
design: High-quality DNA extraction was performed following the protocol proposed by Diaz, Mathiasen and Premoli (2020). Libraries for Illumina genotyping by sequencing (GBS) were prepared following the procedure described by Poland et al. (2012) in the plateforme d'analyses gnomiques of the Institut de Biologie Intgrative et des Systmes (IBIS, Universit Laval, Qubec, Canada), and SbfI/MspI were used as restriction enzymes. Plate barcoding was used on a single lane of an Illumina HiSeq 4000 as described in Colston-Nepali et al. (2019). Libraries were optimized for paired-end sequencing. Sequencing was performed at Genome Qubec Inc. in Montreal, Canada.