title: sRNA mock-inoculated tomato leaf 24 hpi
design: Oligonucleotide adapters were ligated to the 5' and 3' ends of the small RNA samples. First-strand cDNA synthesis was performed using M-MLV reverse transcriptase and the 3 adapter as primer. The resulting cDNAs were amplified with PCR using a high fidelity DNA polymerase. The cDNA was purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics) and was analyzed by capillary electrophoresis and sequenced on an Illumina NextSeq500 platform.