title: GSM8171767: m_COPD_3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted by Trizol. Total amount of 1.5 ug RNA per sample was used as input material for the RNA sample preparations.For tsRNA：total RNA samples are pretreated to remove some RNA modifications that  interfere with small RNA-seq library construction. For RNA-Seq: Total RNA samples were processed by oligo dT enrichment (rRNA removal) and then KAPA Stranded RNA-Seq Library Prep Kit (Illumina) was used to construct the library, and Agilent 2100 was used for quality control. For tsRNA-Seq, the extracted total RNA was subjected to pretreatment using the rtStar™ TRF&tiRNA pretreatment kit (Arraystar, USA) and then sequencing libraries was  prepared utilized the rtStar™ FirstStrand cDNA synthesis kit (3ʹ and 5ʹ adapters) (Arraystar, USA) .