title: GSM7528131: 403_DC12W + Lixi; Rattus norvegicus; RNA-Seq
library_construction_protocol: After a total duration of hyperglycemia of 12 weeks, rats were killed, and the eyes were obtained and stored at -80°C until further use. Total retinal RNA was isolated using Trizol reagent according to the manufacturer's protocol (Invitrogen, Carlsbad, CA, USA). Six retinae total RNA samples from each group were randomly selected for RNA-sequencing to obtain an unbiased view of retinal transcriptome. mRNA-focused library preparation with total RNA and using an Ilumina sampe prep kit. Thereafter, single-end sequencing with 50bp reads. Per sample 40x10E6 to 50x10E6 reads were obtained.