title: RAD-seq of phyteuma nigrum: leaf
design: Preparation of RAD libraries followed Paun et al. (2016) except using PstI  for digestion, 150 ng of DNA as starting material, and taking 72 individuals per library. A final size selection was done with a Pippin Prep (Sage Science, Beverly, MA, USA) targeting the size range 250-850 bp.  Libraries were sequenced on the Illumina HiSeq2000 v4 platform using single-end 100 bp reads at the the Next Generation Sequencing Facility at Vienna BioCenter Core Facilities