title: GSM6761428: non-treated 4 h biol rep 2 [C4h2]; Mus musculus; RNA-Seq
library_construction_protocol: Live CD4+ T cells were sorted on a FACS Aria cell sorter and collected into eppendorfs. Cells were lysed in 100uL extraction buffer using PicoPure RNA extraction kit according to manufacturer's instructions. An on column DNase digest was performed. Libraries were constructed using Lexogen Quantseq 3' mRNA Forward kits according to the manufacturer's instructions using a starting amount of 5ng of RNA and UMIs 3' mRNA-seq FWD UMI