title: Nanopore sequencing of Bipolaris sorokiniana ND93-1
design: High molecular weight DNA was extracted from the fungus using the DNeasy Plant Mini Kit (QIAGEN). The DNA was size-selected to greater than 15Kb fragments with a Blue Pippin.  The size-selected DNA was used to make libraries with the Nanopore Sequencing Kit ( SQK-LSK109) following the manufacturers protocol and sequenced on a R9.4.1  flowcell. GPU-basecalling was performed with the guppy 3.6.1 high-accuracy model.