title: RNA-Seq of dendronotus iris: adult brain and eyes
design: Dissected brains from 15 animals were flash-frozen and pooled, and total RNA was extracted using a QIAGEN RNeasy Plus Universal Midi Kit. RNA quality was assessed with a Bioanalyzer 2100 (Agilent) and sent to Beckman Genomics where messenger RNAs were isolated from each sample using poly-A capture, converted to cDNA, and Illumina sequencing adaptors were ligated for sequencing on an Illumina HiSeq 2500.