title: GSM6670855: SQR_2_S_STRIGA_REP3; Sorghum bicolor; RNA-Seq
library_construction_protocol: Root tissue was manually ground and RNA was extracted with RNaesy Plus Mini kit (Qiagen) with application of QIAshredder columns (Qiagen) and on-column Dnase I (Qiagen) treatment. Total RNA obtained was precipitated with 3M NaOAc pH 5.2 (Thermo Scientific) in 100% ethanol and washed with 70% (v/v) ethanol RNA‑seq libraries were synthesized with QuantSeq 3' mRNA‑Seq Library Prep Kit (Lexogen) according to manufacturer protocol.