title: GSM8128026: panicles, MOCK_B5; Oryza sativa; ChIP-Seq
library_construction_protocol: MNaseI based chromatin was prepared with DNA fragments ranging from 150bp to 900bp. For the ChIP, 8μg of chromatin was used and incubated with 6μg of affinity-purified anti-OsMADS2 antibody. The DNA-protein complexes were pulled down using protein-A magnetic beads.  Simultaneously, a negative control sample using rabbit IgG antibody (mock IP) instead of the  anti-OsMADS2 antibody was processed in the same way. Immunoprecipitated DNA was eluted using the elution buffer provided in the kit and reverse cross-linked using 2M RNase A  and 4M proteinase K in 5M NaCl for 6 hours at 65˚C. Reverse-crosslinked ChIP DNA was purified using the phenol/chloroform purification and precipitation method. Approximately 6ng of ChIP DNA was processed to prepare a library for NGS by using the Kapa kit, library amplification was performed before the double-size selection. ChIP-Seq was performed using the Illumina HiSeq2500 machine at the Functional Genomics Center Zurich