title: GSM6928182: mESC-dPiga-H3K4me3-BS03468A; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were dissociated using TryPE-Select, spun-down and media aspirate. Pellets were either processed immediately or stored at -80 deg. Genomic DNA was extracted using the DNeasy Blood & Tissue kit (QIAGEN 69506). Sheared chromatin from crosslinked mESCs was immunoprecipitated using H3K4me3 antibody (Diagenode C15410003-50) conjugated to M-280 Dynabeads (Invitrogen 11204D), followed by reverse crosslinking and treatment with Proteinase K/RNase A prior to DNA library preparation.