title: GSM8432720: M 7_WT+library; Mus musculus; OTHER
library_construction_protocol: DirectPCR Lysis reagent (Viagen Biotech #301-C) SgRNA sequences were  amplified using NebNext High-Fidelity 2X PCR Master Mix (New England Biolabs #M0541S), following the manufacturer's instructions, using primers containing sample barcodes