title: soil with cow dung, composted by dung beetles, replicate no.2
design: Specific primers were used for amplification of the V4-V5 region of 16S rDNA, which resulted in amplification of fragments of ~ 420 bp. After adapter linkage, the Illumina NovaSeq platform was used to obtain 2 x 250 bp paired-end sequences. Longer sequences for 16S rDNA could be obtained through read splicing.