title: GSM7116297: wild type replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: retinas were dissected in cold PBS(311-010-CL, Wisent), enucleated and cutinto 2mm pieces. Retinal tissues were dissociated intosingle cell suspensionsusingaPapain dissociation system (LK003150, WorthingtonBiochemical Corp) according to the manufacturer's instructions andthenresuspended in PBSwith 0.04% BSA. Dead cells were eliminated using a Dead Cell Removal Kit (130-090-101,Miltenyi Biotec) with MS columns and a gentleMACS™ Octo Dissociator according to themanufacturer's instructions. Cell number and viability were determined usingaCountess®II FLAutomated CellCounter (ThermoFisherScientific). Cells were diluted in 1X PBS with 0.04%bovine serum albumin (BSA)to 1X 10^6 cells/ml and cellular concentration and viability wereaccessed again, withonly samples with 90% viability and higherused. Live cells were processedusing theChromium Next GEMSingleCell 5'ReagentKit v2 (10X genomics, Cat # 1000263).For each reaction, 16500 cells were loaded onto GEM Chip K for an expected recovery of10,000 cells. Gel Beads-in-emulsion were generated using the Chromium Controller followed bycDNA generation and amplification (13 cycles) as per manufacturer's instructions. For eachsample, 50ng of cDNA was used for library generation