title: GSM8498491: tumor, ctrl, rep1, CSP; Mus musculus; OTHER
library_construction_protocol: Tissues were harvested and samples were transferred into microcentrifuge tubes containing 1 mL TRIzol. RNA was then isolated using Qiagen RNeasy Mini Columns (74004; Qiagen, Germantown, MD) according to the manufacturer's recommendations and including an on-column DNase I digestion. Yield and integrity of RNA was confirmed by reading absorbance at 260, 280, and 230 nm using a NanoDrop ND-1000 (NanoDrop Technologies; ThermoFisher Scientific, Inc., Wilmington, DE, USA) and with the Agilent 2200 Tapestation (Agilent Technologies, Inc.). Library preparation and paired-end 150 bp (PE150, Illumina) RNA sequencing was performed by Novogene (Novogene Corporation, Sacramento, USA).