title: WGS of VLP isolated from Schneider's medium (supplemented with 20% FBS and 1% L-glutamine)
design: Virus-like particles are isolated from samples by centrifuging culture supernatant and filtering through a 0.2uM filter, with the filtrate concentrated in SM buffer using Amicon-15 centrifugal filter and incubated with DNase I and RNase. Nucleic acid was extracted using AllPrep DNA/RNA Mini Kit. RNA was reverse transcribed into cDNA using a SuperScript III First-Strand Synthesis System Kit.