title: GSM8619206: wildtype mouse gingival tissue, injured, Day 3, biol rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Samples are total RNAs that were extracted from oral tissue using Qiagen miRNeasy Mini Kit. They are all collected at one time point. For Total RNA sequencing service, RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Ribosomal RNA was removed, followed by fragmentation with divalent cation buffers in elevated temperature. The sequencing library was prepared following Illumina's TruSeq-stranded-total-RNA-sample preparation protocol. Quality control analysis and quantification of the sequencing library were performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA Chip. Paired-ended sequencing was performed on Illumina's NovaSeq 6000 sequencing system. The following diagram depicts the workflow of library preparation for total RNA sequencing. Total RNA Sequencing