title: 16S V3-V4 amplicon sequencing for freshwater. DNA extracted with the Magen HiPure Bacterial DNA Kit (#D314602 Magen); biological replicate 2
design: Amplification of the V3-V4 region of 16S rRNA and library preparations were performed according to the Illumina manual (16S Metagenomic Sequencing Library Preparation. Part # 15044223 Rev. B). The amplicon libraries were barcoded, pooled in a single batch and sequenced in Evrogen using NovaSeq 6000, 2 x 250 bp paired-end protocol.