title: RNA-seq of Homo sapiens: ABCG2+ L3.6pl tumorspheres
design: Total RNA was extracted from ABCG2+ L3.6pl tumorspheres with Trizol reagent (Thermo Fisher Scientific, cat. 15596018) and purified using Direct-zol RNA miniprep kit (Zymo Research, cat. R2050) according to the manufacturers instructions. Ribosomal RNA (rRNA) was depleted using NEBNEXT rRNA Depletion Kit v2 (Human/Mouse/Rat) (New England Biolabs, cat. E7400S) as per the manufacturers instructions. RNA-seq libraries were prepared using NEBNEXT Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, cat. E7760S) according to the manufacturers instructions. Fragment size distribution of cDNA libraries was determined using Agilent 4150 TapeStation system (Agilent Technologies). RNA-sequencing of 3 biological replicates per each treatment condition was performed at CD Genomics (New York, USA) using Illumina Hiseq with paired-end 150 bp (PE150) platform.