title: GSM8215355: HSC_matrigelmonoculture_day7_C1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using a RNeasy Micro Kit (Qiagen, Hilden, Germany) including an on-column DNase-I treatment to minimize DNA contamination. The libraries were prepared following the protocol for Smart-seq2. Following reverse transcription, an Agilent 4200 TapeStation System was used to evaluate the quality of the cDNAs. Using an Illumina Nextera XT kit 1.0 ng of amplified cDNA from each sample was tagmented and amplified. The resulting libraries were pooled, double-sided size selected (0.5x followed by 0.8x ratio using Beckman Ampure XP beads) and quantified using an Agilent 4200 TapeStation System.