title: Labeo catla differential Gene expression analysis of Brain tissue
design: The sequencing using Illumina generated raw reads were pre-processed using Fastp v.0.20.1, FastQC and summarization using MultiQC. The reads were filter bowtie2 v2.4.2 and  assembled using Trinity v2.13.2 followed by clustered using CD-HIT-EST. The transcripts was indexed using Kallisto v0.43.1 and DESeq2 used for DGE analysis