title: Genomic DNA of Hippoboscas camelina male
design: The sequencing library was prepared using the Ligation Sequencing Kit (SQK-LSK109). High molecular weight genomic DNA (gDNA) was extracted, and its quality and quantity were assessed. For the library preparation, 1 g (or 100-200 fmol) of gDNA was used. In cases where DNA fragmentation was necessary, the input amount could be reduced to as low as 100 ng. The process began with size selection of the DNA, followed by an end-prep and nick repair step. During this stage, the DNA ends were repaired and prepared for adapter attachment. The next step involved the ligation of sequencing adapters, using components provided in the kit, to the prepared DNA ends. The ligation reaction included Ligation Buffer (LNB), NEBNext Quick T4 DNA Ligase, and Adapter Mix (AMX). The reaction mixture was incubated to facilitate the attachment of the adapters. After ligation, the library was cleaned up using  AMPure XP beads to remove excess adapters and other reaction components. The cleaned library was eluted in Elution Buffer (EB) and quantified using a Qubit fluorometer. The final prepared library was loaded onto a SpotON Flow Cell using the appropriate amount of Sequencing Buffer (SQB) and Loading Beads (LB), as per the MinION device requirements.