title: GSM8170613: day 6 post-infection brains, pooled mice; Mus musculus; RNA-Seq
library_construction_protocol: Myeloid cells from the brain were sorted as live CD45+CD11b+ singlets, and collected into RPMI media supplemented with 10% FBS and 1% Pen/Strep, keeping cells cold during the sort. Sorted cells were spun down and resuspended into PBS supplemented with 0.04% BSA at a concentration of approximately 1000 cells/uL. Cells were loaded on a GemCode Single-Cell Instrument (10X Genomics) to generate single-cell gel bead-in emulsions (GEMs). Library was constructed according to manufacturer's instructions (single cell 3' v2 or v3 protocol, 10x genomics)