title: GSM7868612: L642A/W161A/T159A PADI2_2; Homo sapiens; RNA-Seq
library_construction_protocol: GFP-positive singlet cells and live cells were suspended in 1ml of PBS+BSA 0.05%. Cells were centrifuged at 400 rcf during 5 min at 4° in order to bring the cell concentration to 300-1000 cells/µl. Cell concentration and viability were determined by manual counting using a Neubauer chamber and staining the cells with Trypan blue. Cells were partitioned into Gel Bead-In-Emulsions (GEMs) by using the Chromium Controller system (10X Genomics), with a target recovery of 5000 total cells per sample. NovaSeq 6000 using the following sequencing conditions: 28 bp (Read 1) + 10 bp (i7 index) + 10 bp (i5 index) + 90 bp (Read 2), to obtain approximately 20-30.000 reads per cell.