title: Primary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Bulk RNAseq
design: Primary human CD34+ cells for modeling ETO2-GLIS2 leukemogenesis - Bulk RNAseq
library_construction_protocol: FL or CB CD34+ cells subjected to lentiviral transduction with a CTRL or ETO2::GLIS2 encoding particles, selection in vitro or in vivo where enriched for GFP+ cells by flow sorting Cells were lysed and RNA extract using RNeasy MiniKit (Qiagen).  cDNA libraries were synthesized from 250 ng total RNA using TruSeq Stranded mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).