title: GSM7797869: 6B_53192_V_epi; Mus musculus; RNA-Seq
library_construction_protocol: Samples were collected in 1 mL of TRIzol Reagent and stored at -80C. The RNA was extracted using the RNeasy Micro kit (74004; Qiagen) For the PCR steps, we performed 14 cDNA PCR cycles for samples <1ng; 11 cDNA PCR cycles for samples at <10ng; and 8 cDNA PCR cycles for samples between >10ng an <100ng.For the final PCR step, we performed 9 PCR cycles for samples <10ng and 6 PCR cycles for samples >10ng. the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420S) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pair) (E6440S)