title: RNA-seq of An. darlingi adult female
design: Tissue from mosquitoes grouped in each pool was homogenized in 750 l of TRIzol (Thermo Fischer) using tungsten carbide beads in a TissueLyser2 equipment (Qiagen). Total RNA extraction was performed with QIAzol lysis reagent (Qiagen), and the resulting extract was purified using RNeasy Mini Kit columns (Qiagen). Subsequently, the cDNA library was prepared with the Illumina TruSeq stranded kit for total RNA (Illumina). To enrich non-ribosomal RNA, the Ribo-Zero-Gold kit (Illumina) was utilized. Sequencing was conducted in paired-end mode on a NovaSeq6000.