title: GSM8442446: miR-342-3p sponge - biological replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Cells were sorted with the Flow cytometry (FC) before sequencing.Adherent cells were washed with PBS and incubated in 0,05% trypsin for 3min 37°C, then detached by pipetting and collected in FACS tubes. 500k-1mln cells/sample were sorted according to mOrange positivity and viability. After sorting, cells were pelleted 300g for 5min 4°C, resuspended in 700uL QIAzol buffer and total RNA was extracted with miRNeasy Micro kit (QIAgen/ 217084) following manifacturer instruction. Libraries were prepared from total RNA as service by Genewiz-Azenta.