title: GSM7868660: 22_doa10_unstable_rep2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Yeast cells were lysed by mechanical shearing and then the plasmids therein were isolated by column-purification. The variable regions were PCR-amplified with the incorporation of unique barcodes for each stability bin. The PCR products were pooled proportionally according to the number of cells sorted into each bin, and then used as the template for another PCR for adding the sample barcodes and illumina sequencing adaptors.