title: PacBioHiFi of Mesembryanthemum crystallinum: leaf
design: Genome DNA was extracted from of M. crystallinum leaves using Nucleobond HMW DNA Kit (Takara-Bio, Kusatsu, Japan) according to an instruction manual. DNA purity was determined by a Qubit Fluorometer. PacBio nanopore HiFI Library construction and sequence was done with SQK-LSK112 (Oxford Nanopore Technologies, Oxford, UK) kit according to the protocol modified by (Naito, 2023) and sequence was performed on PromethION R10.4 flowcell (Oxford Nanopore Technologies, Oxford, UK) according to the instructions.