title: GSM8497720: tibia, DEX control, 4; Mus musculus; RNA-Seq
library_construction_protocol: The frozen bone tissue was pulverized under liquid nitrogen using a Freezer/Mill 6775 (SPEX). For the extraction of RNA, TRIzol reagent was applied to the crushed tissue, followed by chloroform addition for phase separation. The RNA-containing aqueous phase was isolated and purified using the Direct-zol MicroPrep kit. Total RNA was extracted from mouse tibiae and used for library construction using the SMARTer Stranded Total RNA-Seq Kit v2—Pico Input Mammalian (Takara Bio, 634412).