title: T2_CARBC_NGS
design: Genomic DNA from CAR T cells harvested directly from in vivo tissues was isolated using a PureLink Genomic DNA Mini Kit (ThermoFisher), following manufacturers instructions. CAR BCs were amplified using primers specific for the 5UTR within the CAR constructs, which also included handles with Illumina-compatible adapters. PCR reactions were purified using AMPure XP beads (Beckman), following manufacturers instructions, short-read sequencing via the Genewiz Amplicon-EZ service.