title: CpA5T_2
design: Initial quantification is performed with Qubit2.0 Fluorometer and the library is diluted to 1.5ng/ul. The insert size of the library is then tested with Agilent 2100 bioanalyzer. qRT-PCR can accurately quantify the effective concentration of the library (the effective concentration of the library is higher than 2nM) to ensure the quality of the library.