title: RNA-seq of Cobalt and Nickel treatments to HepG2 cells
design: RNA-seq of Cobalt and Nickel treatments to HepG2 cells
library_construction_protocol: cells were pelleted using 0.25 % trypsin-EDTA for detaching, followed by washing with PBS and centrifugation at 2370 x g and 4 Â°C for 4 min.  After removing the supernatant, total RNA of the cell pellet was isolated using RNeasy Plus Mini Kit which includes DNAse digestion (Qiagen).  Poly-A RNA libraries were prepared using the D-Plex mRNA-seq Kit for Illumina (Hologic Diagenode)