title: GSM8301850: AMW; 1:1; 9-143-16; Homo sapiens; OTHER
library_construction_protocol: Cellular or plant total RNA was extracted from cells or plant tissue using TRIzol reagent (Ambion by Life Technologies) and Direct-zol RNA miniprep kit (Zymo Research) following the manufacturer's protocol. After RNA extraction, two rounds of Poly(A) enrichment were conducted using DynaBeads mRNA Direct Purification kit (Thermo Fisher Scientific) following the manufacturer's protocol with some modifications. Following the CAM-seq treatment, RNA samples were purified and sequencing libraries were prepared using the cDNA ligation method. Briefly, the RNA was end-repaired and ligated with a 3' adapter, then reverse transcribed into cDNA under various enzymatic and RT conditions. Subsequently, the cDNA was ligated with a cDNA adapter. The sequencing libraries were then amplified using a dual-index primer for Illumina sequencing.