title: 16S rRNA: Tank D3 Surface Week 2
design: eDNA material was collected by filtering water samples using 0.22um GF/C filters (Whatman, USA). DNA was stored under -80C until further extraction. DNA was later extracted using DNEasy Power Water Kit (QIAGEN, Germany). Obtained DNA concentration was measured using Nanodrop spectrophotometer (Thermo Fisher Scientific, USA). Sequencing library was prepared using 16S Barcoding Kit 1-24 (SQK-16S024) (Oxford Nanopore Technologies, UK). Obtained PCR products were purified using AMPure XP beads (Beckman Coulter, USA) and later sequenced on a MinION Mk1C sequencing platform