title: GSM6928351: RNA-Seq FZX_Le_C1; Litchi chinensis; RNA-Seq
library_construction_protocol: Total RNA was extracted from the treated leaves by Plant RNA Kit (Promega, LS1040). In the construction of Strand-specific RNA-seq (removal of ribosomal RNA), the epicenter Ribo-ZeroTM kit was used to remove the ribosomal RNA. Then, rRNA-depleted RNA was fragmented and used as a template to construct the cDNA library.The qualities of the library was further tested following these steps: 1) Initial quantification was performed using Qubit 2.0, and the insert size of the library was tested using Agilent 2100. 2) The Q-PCR method was used to accurately quantify the effective concentration of the library (effective library concentration > 2 nM). After the library's quality tests were passed, the library performed pooling according to the amount of target data, followed by sequencing on the Illumina NovaSeq platform. ssRNA-Seq (stranded RNA-seq)