title: COI amplicon sequencing of bulk insect sample
design: Amplicon libraries were prepared using a two-step PCR approach, as described in Marquina et al. (2021). We amplified a 458-bp region of the COI gene using BF3-BR2 primers (Elbrecht et al., 2019) and sequenced the library pools on Illumina NovaSeq 6000 (2 x 250bp reads).