title: GSM6928338: Ascites_BEX_14; Homo sapiens; RNA-Seq
library_construction_protocol: After the 48h antibody treatments, ascites cells were washed twice with 0.04% BSA-PBS, filtered through 70 μm nylon nets and resulting cell suspensions subjected to single-cell RNA-sequencing. Single-cell partitioning and library preparation were done according to manufacturer's instructions using Chromium Next GEM Single Cell 3' GEM Library & Gel Bead Kit v3.1 and Chromium Next GEM Chip G Single Cell Kit (10X Genomics). For batch 1, single indexes were used (protocol CG000204) and for batch 2, dual indexes were used (protocol CG000315).