title: GSM7869238: culture_eTIP_WT_500uM_ETOP_TOP2B_A300-950A_3; Mus musculus; OTHER
library_construction_protocol: Acute cortical slices were treated with 0.5mM etoposide (VP-16) for 15 minutes at 37oC. To fragment DNA, the lysates were sonicated with Covaris E220 sonicator (5% Duty Factory, 140 Peak Incidence Power, 200 cycles per burst, milliTUBE 1mL AFA Fiber). eTIP was performed with TOP2B (Fortis Life Sciences, A300-950A) or TOP2B (Santa Cruz, sc-13059). At 12 days in vitro (DIV12), primary neuron cultures were treated with etoposide (VP-16) and incubated at 37oC. To fragment DNA, the lysates were sonicated with Covaris E220 sonicator (5% Duty Factory, 140 Peak Incidence Power, 200 cycles per burst, milliTUBE 1mL AFA Fiber). eTIP was performed with TOP2B (Fortis Life Sciences, A300-950A) or TOP2B (Santa Cruz, sc-13059). eTIP libraries were generated using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences). Libraries were pooled to a final concentration of 4-10nM and reads were generated on the Illumina HiSeq 3000 with the Genome Technology Access Center or generated on the Illumina Nextseq 500 with the Center for Genome Science at Washington University in St. Louis, typically yielding 15-40 million single-end reads per sample.