title: GSM7360734: Calu3 cells - Andes virus + 6AZA - 48hrs - rep 3 (Drug study) [Sample 46_AA-3]; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were harvested in TriZol and RNA was extracted using the BioRad RNA Mini kit Libraries were prepared with KAPA Stranded mRNA-Seq Kit followed by second strand synthesis converting cDNA:RNA hybrid to double-stranded cDNA (dscDNA), and incorporates dUTP into the second cDNA strand.  cDNA generation is followed by end repair to generate blunt ends, A-tailing, adaptor ligation and PCR amplification. Different adaptors were used for multiplexing samples in one lane.