title: RNA-Seq of mus musculus: kidney
design: The library construction method and sequencing process are carried out according to thefollowing steps: 1.Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNAwith poly A tail;2. mRNA molecules were fragmented into small pieces;3. The fragmented mRNA was synthesized into first strand cDNA using random primers;4. The second strand cDNA was synthesized with dUTP instead of dTTP;5.The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors wereligated to the ends of these 3' adenylated cDNA fragments;6.Digest the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG) andperform PCR amplification;7. Library quality control;8. Library circularization;9. The library was amplified to make DNA nanoball (DNB);10. Sequencing on DNBSEQ (DNBSEQ Technology) platform.