title: GSM8217319: E10.0 posterior; Monodelphis domestica; RNA-Seq
library_construction_protocol: Samples were incubated on FACSmax Cell Dissociation Solution (Amsbio, T200100) with 10% Papain (30 U/mg, Sigma-Aldrich, 10108014001) for 11-12 min at 37°C to dissociate the cells. Samples were then resuspended in HBSS with 5% FBS, 10 µM rock inhibitor (Stemcell Technologies, Y-27632), and 1x non-essential aminoacids (Life Technologies, 11140050). Cells were filtered through 35 µm cell strainers (Falcon, 352235) and 20 µm filters (Miltenyi Biotec, 130-101-812). Libraries were prepared using the Single Index Kit T Set A (PN-1000213) from the 10X Genomics Chromium Next GEM Single Cell 3' Kit v3.1 (PN-1000121)