title: 16S rRNA of sludge microorganisms: denitrification system sludge
design: Library construction was performed using the NEXTFLEX Rapid DNA-Seq Kit: (1) junction linkage; (2) removal of junction self-associated fragments using magnetic bead screening; (3) enrichment of library templates using PCR amplification; and (5) magnetic bead recycling of PCR products to obtain the final library.