title: GSM8433495: RAB1430 - CONTROL rep1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized at 15 weeks old and liver were extracted. Total RNA samples (500ng; RQS/RIN average 7.1, range 5.9-8.6) were converted into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015). Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. Primers from both steps contain Illumina-compatible sequences. Libraries were completed by PCR, and sequenced on an Illumina instrument.