title: GSM8377616: OVCAR8 Control rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated by using TRIzol (Invitrogen) according to the manufacturer's instructions and quantified using a NanoDrop 3300 Fluorospectrometer. The RNA-sequencing (RNA-seq) libraries were prepared from 1 μg of total RNA of OVCAR5, and OVCAR8 mono- or co-culture. Libraries were generated using the NEBNext Ultra II RNA library prep kit from Illumina (New England Biolabs, Inc.). Library preparation was performed accoding to manufacter's protocol.