title: GSM6761291: LPS_2h_R3; Mus musculus; RNA-Seq
library_construction_protocol: chromatin-associated-RNA-seq libraries were prepared for NextSeq500 sequencing following standard protocols. Library preparation was performed following the SMART-seq2 protocol (Picelli et al. 2014b). Briefly, 5 ng of chromatin associated RNA were reverse transcribed with template-switching using random primers and an LNA-containing template-switching oligo (TSO). The resulting cDNA was pre-amplified, purified and tagmented with Tn5 transposase produced in-house. cDNA fragments generated after tagmentation were gap-repaired, enriched by PCR and purified to create the final cDNA library for Illumina NextSeq500 sequencing. RNAseq_Smartseq2