title: GSM8127402: iPSC1 CL1 Producvie ATACseq; Homo sapiens; ATAC-seq
library_construction_protocol: Extraction was carried out when individual hiPSC clones achieved 70~90% confluency, indicating suitability for passaging or cardiomyocytes differentiation. Cell collection was facilitated using 0.5 mM EDTA. Initially, cell counts were prepared for ATAC-seq, with the remaining cells utilized for RNA-seq. ATAC libraries for ATAC-seq were prepared using Omni-ATAC-seq protocol (J. D. Buenrostro et al., Current Protocols in Molecular Biology, 2015). Breifly, nuclear isolation was performed using lysis buffer, and DNA was transposed using Illumina Tn5 transposase, followed by index PCR for tagmentation-based NGS library preparation.