title: intestinal microbiota of juvenile European seabass
design: Bacterial DNA was extracted from the whole intestinal contents of 15 juvenile European seabass fed with test diets (3 replicates per treatment). For DNA isolation we used the Invitrogen PureLink Microbiome DNA Purification Kit (Carlsbad, CA, USA) according to the manufacturers protocol. Library preparation was based on Nextera's two-step PCR, including purification and pooling. The variable region V4 of the 16S rRNA gene was sequenced with the MiSeq2000 Next Generation System (Illumina, San Diego, CA, USA) by the commercial service of Microsynth AG (Balgach, Switzerland).