title: First day sample after Fusarium graminearum inoculation replication 3
design: Total RNA was extracted using Trizol reagent (Invitrogen, CA, USA), and its integrity was estimated by Agilent Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) through measuring the RNA integrity number (RIN). Total RNA was digested with Ribo-Zero rRNA Removal Kit (Epicentre, Madison, WI, USA) to obtain rRNA-depleted RNA, and then was digested with RNase R (Epicentre, Madison, WI, USA) to remove linear RNAs. Remaining RNA was used for library construction using NEB Next Ultra Directional RNA Library Prep Kit (NEB, USA).