title: MSG of Erythranthe guttata x Erythranthe nudata F2s
design: 50 ng of genomic DNA from each individual was digested with the restriction enzyme Csp6I and 96 custom-designed adapters, each carrying a unique 6 bp barcode, were ligated to the restriction fragments. The ligation reactions were combined, fragments were size-selected, and 8 standard Illumina flow-cell adaptors were added using PCR with custom designed primers.