title: GSM8377623: WT3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted with the standard TRIzol RNA extraction protocol. RNA quality and quantity were evaluated using a 2100 bioanalyzer (Agilent) and the Quant-iT RiboGreen RNA Assay Kit (Thermo Fisher, R11490). One hundred nanograms of RNA was used to generate the libraries using the TruSeq Stranded mRNA kit (Illumina, 20020594). These libraries were sequenced on an Illumina NovaSeq sequencer on an SP flow cell.