title: adhering community of PS group at 5 weeks #4
design: The DNA from the plastic (biofilm) and bacterial suspension (planktonic bacteria) samples was extracted using the DNeasy Powersoil Kit (Qiagen, Germany) and cleaned up using the Genomic DNA Clean & Concentrator (ZymoResearch, USA) following the manufacturers protocols. The V4 and V5 regions of 16S rRNA genes were amplified using the primers 515F (5-GTGCCAGCMGCCGCGG-3) and 907R (5-CCGTCAATCMTTTRAGTTT-158)