title: Raw reads: Trichechus manatus brain IsoSeq library
design: PacBio IsoSeq library created from cDNA extracted from West Indian manatee brain. To create read clusters, ccs reads were called using pacbio ccs, including those reads with a read quality of 90%. PolyA tails were trimmed and rapid concatemers were identified and removed using pacbio isoseq refine then clusters were identified using pacbio isoseq cluster2. The reads contain high quality (predicted accuracy greater than 99%) and low quality (predicted accuracy less than 99%) clusters