title: RNA-Seq of C.elegans: L4 WT
design: Total RNA was extracted using the RNAiso Plus kit from Takara. The concentration of RNA samples was determined using the Bioanalyzer system (Alignment 2100). RNA library construction was followed the protocol provided by New England Biolabs' NEBNext Ulitra RNA Library Prep Kit for Illumina. Sequencing was carried out on an Illumina HiSeq2000 instrument.