title: GSM8125770: PLF-PBS; Mus musculus; RNA-Seq
library_construction_protocol: Mice were immunized intraperitoneally (i.p.)  with or without 10 µgTLR stimulation in 100 µL PBS, peritoneal cavity cells were obtained by peritoneal cavity lavage twice with 5 mL of ice-cold PBS. They were collected 6 hrs after injection for cDCs (live MHCII+ CD11c+) FACS-sorting for scRNA-seq library preparation. The scRNA-seq libraries were constructed using the Chromium Single Cell 3ʹ Library, Gel Bead & Multiplex Kit (10x Genomics, V3.1) following the manufacturer's instructions.