title: sbcer6/sbkcs6 at boot stage RNA-seq_replicate2
design: WT, bm41, and sbcer6 plants were grown under normal conditions in a greenhouse (as described above). Total RNA was extracted from leaf sheaths at the boot stage and growing point differentiation stage for WT, at the growing point differentiation stage for bm41 and boot stage for sbcer6, using the RiboPure RNA Purification Kit (AM1924; Invitrogen), according to the manufacturers instructions. Standard Illumina RNA-Seq library preparation and RNA-Seq were performed by a commercial sequencing provider. The RNA-seq reads were aligned to the sorghum BTx623 v3 reference genome using STAR (Dobin et al., 2013).