title: GSM8127423: MGG4 input, BRD9, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using Bioruptor Pico (Diagenode). ChIP-seq libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina complemented with the NEBNext Multiplex Oligos for Illumina. 2-5 ng of DNA was used as starting material for input and IP sample. Libraries were amplified using 14 cycles on the thermocycler. Post amplification libraries were purified using AMPure XP beads from Beckman Coulter. Libraries were validated using the Agilent High Sensitivity DNA Kit.