title: GSM6760632: Knockout Mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Bladders were collected and stored in RNA later (Qiagen, Valencia, CA). Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA) and treated with DNAse I (Ambion, Austin, TX) to remove genomic DNA. Preparation of sequencing libraries and Illumina sequencing were carried out at Novogene (Sacramento, CA). Briefly, sequencing libraries were built using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, MA, USA)