title: GSM6668928: P2_TN; Homo sapiens; RNA-Seq
library_construction_protocol: We used RosetteSep T cell enrichment kit (StemCell) to isolate untouched CD3 T cells. Each sample was washed once with RPMI and 3 times with PBS + 2% BSA before labeling and pooling. Specimens were then filtered through 70 um strainers (Fisher). Cell concentration, singularity, and viability were confirmed with a hematocytometer before submission for scRNA-Seq (10X Genomics). This experiment was done using the Chromium Single Cell V(D)J Reagent Kits (v1 Chemistry) AKA 5'