title: RNA-seq of O. vittatus: tail sample
design: Tissue samples were removed from RNALater and homogenized using a TissueRuptor (Qiagen). Total RNA was extracted from each sample with TRI Reagent (Sigma) using the manufacturers protocol. The concentration and integrity of RNA were examined using a Thermo-Fisher Scientific NanoDrop 8000 Spectrophotometer and an Agilent 4150 TapeStation. PolyA-selected RNA-seq libraries were prepared at g-INCPM, Weizmann Institutre of Science, Rehovot, Israel using the INCPM-mRNAseq protocol.