title: GSM8443017: SI, 20w Naïve WT, rep1; Mus musculus; RNA-Seq
library_construction_protocol: tissue RNA were extracted by TRIzol reagent (Invitrogen) following manufactory instructions. RNA samples are denatured at suitable temperature to open their secondary structure, and mRNA is enriched by oligo (dT) -attached magnetic beads. RNAs are fragmented, and prepared for cDNA synthesis,then double-stranded cDNA fragments are subjected to end-repair,and ligated with adaptors.the products were amplified, denatured and cyclized, and finally applied for sequencing on BGISEQ-500