title: Illumina ITS2 amplicon sequencing
design: DNA was extracted from each of the 11 bulk community samples separately. Bulk samples (Table 1) were homogenized using sterilized mortar and pestles; and DNA was extracted from ca. two to four g. of homogenized material using the PowerMax Soil DNA Isolation Kit (Qiagen). From each bulk sample, we amplified a portion of the internal transcribed spacer region  the standard barcoding region for fungi [7]  from each meta-community DNA extraction. Specifically, the hyper-variable ITS2 region was amplified using polymerase chain reaction PCR with primers ITS3F (GCATCGATGAAGAACGCAGC) with ITS4R (TCCTCCGCTTATTGATATGC). PCR products were sequenced at RTL Genomics (Lubbock, TX), using 2x300 paired-end sequencing on the Illumina MiSeq platform.