title: R1703
design: Young leaves of single plants from each lettuce accession were collected, immediately frozen in liquid nitrogen, and stored at 80 C. Genomic DNA from leaf samples was extracted using the cetyltrimethylammonium bromide (CTAB) method. Sequencing libraries with an average insert size of 400 bp were constructed to generated paired-end reads (2150 bp) on an Illumina Novaseq 6000 sequencer at Shanghai Personal Biotechnology, Shanghai, China. All libraries were sequenced with 10 depth.