title: 16S V5-V7 amplicons from Schizaphis graminum
design: DNA was extracted using the DNeasy Blood and Tissue Kit for DNA Isolation (Qiagen, Germantown, MD) following its instructions.  PCR was performed with V5-V7 primers 799F (degenerate AACMGGATTAGATACCCKG) and 1193R (ACGTCATCCCCACCTTCC).  The libraries were barcoded, pooled, and sequenced on an Illumina Novaseq 6000 to generate 2x250-base paired-end reads.