title: Strand-specific RNA-seq in Euglena agilis
design: Strand-specific RNA-seq in Euglena agilis
library_construction_protocol: We cultured E. agilis in mAC, AF-6, and C media for mRNA preparation. E Each culture was maintained in 100 mL volume test tubes for more than 3 days using 50 mL of either culture media type at 29°C with aeration (50 mL/min) and constant illumination (100 μmol photon/m2s).  Total RNA was extracted from cells using NucleoSpin RNA (Macherey-Nagel, USA). A sequencing library was constructed using a TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina, Inc.) according to the manufacturer's instructions (TruSeq Stranded mRNA Sample Preparation Guide Rev. E; Illumina, Inc.).