title: GSM7797266: Engrafted_hPSC_Untreated. PAX7-_rep6; Homo sapiens; RNA-Seq
library_construction_protocol: Mice tibialis anterior skeletal muscle was flash frozen and sectioned at 10µm onto glass coverslips Following overnigh hybridization, same day primary and secondary antibody stain: Cyto13 (488), ACTC1 (Rb IgG-555), Lamin AC and Spectrin (Ms IgG2b 594), PAX7 (Ms IgG1-647) Manufacturer protocol was followed except with modifications: 0.025 µg/mL Proteinase K for 5 minutes .  No antigen retrieval.  No 65C baking step.  Primary and secondy antibody staining performed