title: GSM8376935: MH1, CACPR; Mus musculus; RNA-Seq
library_construction_protocol: Kidney nuclei were extracted using standard protocol by Singulomics. Single nucleus RNA-seq for "CACPR" and "Sham for CACPR" samples were performed using the Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (10x Genomics) by Singluomics targeting 10,000 nuclei per sample. The resulting cDNA was profiled on a Bioanalyzer NanoChip (Agilent), then used as a template for library preparation according to the reagent kit protocol. ( https://www.10xgenomics.com/support/single-cell-gene-expression/documentation/steps/library-prep/chromium-single-cell-3-reagent-kits-user-guide-v-3-1-chemistry ) snRNAseq