title: GSM8648076: spleen_5dpi_non-infection_rep4; Gallus gallus; RNA-Seq
library_construction_protocol: Total RNA was extracted with TRIzol reagent.RNA integrity and concentration was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). After the total RNA samples were tested to be qualified, the MGIEasy RNA library preparation kit was used for library construction. Magnetic beads with oligo (DT) were used for enrichment and purification; Fragment buffer was added to the purified mRNA to make its fragment into a short fragment; the fragment mRNA was used as a template for reverse transcription into double stranded cDNA with six base random primers, and then the product was purified. The purified double stranded cDNA was subjected to end repair, addition of dA tail, linker connection and PCR amplification. After the amplified product was qualified, it was denatured and single stranded cyclized, and the cyclized product was inspected.