title: RNA-Seq of Gallus gallus: female embryonic reproductive crest
design: Five samples of the same gender were mixed to build a pool to extract total RNA 1) Eukaryotic mRNA was enriched with magnetic beads with Oligo (dT), which binds to the ployA tail of the mRNA by A-T complementary pairing 2) The mRNA was broken into short fragments by adding fragmentation buffer, and the mRNA was used as a template for synthesising one-stranded cDNA with six-base random primers ( The mRNA was used as a template to synthesise one-stranded cDNA with random hexamers, and two-stranded cDNA was synthesised with the addition of buffer, dNTPs and DNA polymerase I. 3) Purification of the double-stranded cDNA using AMPure XP beads, and the purified double-stranded cDNA was then subjected to end repair, addition of A tails and connection to sequencing junctions. 4) Selection of fragment size by using AMPure XP beads. The final cDNA library was selected by fragment size selection using AMPure XP beads and finally enriched by PCR.Translated with www.DeepL.com/Translator (free version)