title: genome of chicken primordial germ cells: male LINE/M strain
design: the qualified DNA samples are randomly fragmented by Covaris and the fragments are collected by magnetic beads. Adenine are added to 3end of endrepaired DNA fragments before adaptor ligation. The ligation products are then cyclized and then amplified by linear isothermal Rolling-Circle Replication and DNA NanoBall technology. Sequencing of these DNA libraries is performed with BGISEQ sequencing platform.