title: GSM7868541: hiPSC kucg-2 CRIPSRi sgRNA growth screen ctrl rep2; Homo sapiens; OTHER
library_construction_protocol: 5*106 cells were harvested per screen and gDNA was extracted using the Nucleospin Blood Kit (Macherey&Nagel). Per screening condition, 20 μg of DNA was amplified using NEBNext Ultra II Q5 Mix with two primer sets flanking the sgRNA sequence sgRNA-seq with custom sequencing primer