title: GSM8171277: AC16, Non-targeting control siRNA, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated with TRIzol reagent (Thermo Fisher Scientific Inc., MA, USA), followed by RNA purification using the Zymo RNA Clean & Concentrator™ kit (Euroclone, Milan, Italy) according to the manufacturer's instructions. RNA was eluted in RNAse-free water. Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) was used for library preparation following the manufacturer's instructions (library type: fr-secondstrand). Libraries were sequenced on 150bp PE mode.