title: GSM7868427: RCC4, IFNg + TNF treated, biol rep 2, Illumina; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were first washed with ice-cold PBS (Gibco), followed by lysis with Qiazol (Qiagen). Total RNA was extracted using RNeasy RNA extraction kit (Qiagen) with an additional Dnase I digestion (QIAGEN) according to manufacturer's instruction. Poly(A)+ RNA molecules were enriched from total RNA with the Dynabeads mRNA purification kit (Thermo Fisher). Sequencing libraries were generated using Ultra III directional RNA library prep kit (NEB) according to manufacturer's instructions.