title: RNAseq of A. thaliana cytochrome c mutant
design: Library construction and sequencing was performed by BGI Americas Corporation, Cambridge, MA, USA. PolyA RNA was selected using oligo dT beads. First-strand cDNA was performed using random N6-primed reverse transcription, followed by second-strand cDNA synthesis with dUTP instead of dTTP. cDNA was subjected to end-repair and was 3' adenylated. Adaptors were ligated to 3' adenylated cDNA fragments. The dUTP-marked strand was selectively degraded by Uracil-DNA-Glycosylase and the remaining strand was amplified to generate the cDNA library. After PCR amplification, ssDNA was cyclized using splint oligonucleotides and DNA ligase.