title: GSM8170503: WT_Treg_scRNAseq_rep1; Mus musculus; RNA-Seq
library_construction_protocol: For all scRNA-seq experiments, samples were centrifuged (950g for 5 min) and then pellets were resuspended in 1x PBS plus 0.04% BSA (Amresco) at a concentration of 1x106 cells per mL. Single-cell suspensions were loaded onto a Chromium Controller (10x Genomics) to generate 10,000 single-cell gel beads in emulsion per sample, and each sample was loaded into a separate channel. Libraries were prepared using the Chromium Next GEM Single Cell 3' v3.1 (dual index) and Gel Bead kit (10x Genomics).