title: GSM7116184: CRISPR ATRX KO clone KO-A_MSCV IDH1-R132H_rep 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from monolayer cell cultures using miRNeasy Mini kit (Qiagen, Germantown, MD). 300ng of RNA was used for cDNA prep and construction of sequencing libraries using KAPA Stranded mRNA-Seq kit for Illumina platforms - KR0960 - v6.17. Library construction was performed as per manufacturer's instructions on Sciclone G3 Liquid Handler, 10 PCR cycles.