title: lcWGS of Aphanius iberus for MobiSeq protocol: Adult tail fin
design: In order to select the transposable element for MobiSeq, one Aphanius iberus sample (Sample Name: Chi_6) was selected for low-coverage whole-genome sequencing. DNA was isolated using the NZY Tissue gDNA Isolation kit, following the manufacturers instructions. DNA was eluted in a final volume of 50 L. A genomic shotgun library was constructed using the TruSeq Nano DNA Library Prep kit (Illumina) strictly following the manufacturers instructions. The library was then quantified with the Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific), and its fragment size distribution was checked in the Agilent 2100 Bioanalyzer using the Agilent DNA 1000 Kit (Agilent Technologies). Afterwards, the library was sequenced in the NovaSeq 6000 PE150 platform (Illumina), obtaining 50 gigabases of raw data.