title: GSM8648248: Bulk MARS seq- Naïve3; Gallus gallus; RNA-Seq
library_construction_protocol: RNA was isolated using Trireagent 15ng of purified RNA was used for library preparation with the bulk MARS-seq method, involving barcoding of samples by reverse transcription using an oligo dT primer, pooling of samples, and subsequent molecular reactions for linear amplification and preparation for Illumina sequencing