title: scRNA-seq using Smart-seq3xpress of Homo Sapiens: jurkat cells
design: Library preparation was carried out with the improved version V.2. of the SS3X protocol as deposited on protocols.io on 96 sorted Jurkat cells. Only minor adjustments were made in comparison with this version of the protocol. Modifications include adjusting the input of Tn5 transposase enzyme (Diagenode) to 0.03 L per reaction. Additionally, the use of 0.2% SDS to stop the tagmentation reaction and the inclusion of 0.025% Tween-20 within the index PCR mix to counteract the effects of SDS were omitted. Furthermore, the index PCR mix was refined by utilizing only 0.1 M/each of each index primer, and 0.750 ng of tRNA carrier (ThermoFisher) was added to each reaction to enhance yield. The SS3X libraries were also sequenced on two AVITI sequencer runs, generating PE150 reads.