title: GSM7797879: A375-1st; Homo sapiens; RNA-Seq
library_construction_protocol: To extract RNA, cells were gathered and washed once with PBS. The RNeasy Mini Kit (QIAGEN) was employed following the manufacturer's instructions to isolate total RNA. To prevent genomic DNA contamination, an on-column DNase digest was carried out using RNase-Free DNase (QIAGEN) for 15 minutes at room temperature (RT). The concentration and quality of the RNA were assessed using a NanoDrop ND1000 spectrophotometer (PEQLAB Biotechnologie GmbH, Erlangen, Germany). The strandedlibrarywas constructed using theDNBSEQ Eukaryotic Strand-specific mRNA librarykit (BGI Genomics, Shenzhen, China)