title: GSM8301522: Exp1_PKA KD_rep9; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was purified from liver tissue using MagMAX-96 for the Microarrays Total RNA Isolation kit (Ambion by Life Technologies), according to manufacturer specifications. Genomic DNA was removed using MagMAX Turbo DNase buffer and TURBO DNase from the MagMAX kit listed above (Ambion by Life Technologies). mRNA was purified from total RNA using the Dynabeads® mRNA Purification kit (Invitrogen). Strand-specific RNA sequencing (RNA-seq) libraries were prepared using the KAPA mRNA-Seq Library Preparation Kit (Kapa Biosystems). Twelve-cycle PCR was performed to amplify libraries.