title: GSM6668412: Untreated untransformed library, replicate 1; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Cells washed 3x in sterile water, then DNA extracted with the "smash and grab" protocol of Hoffman and Winston. Polymerase chain reaction was used to amplify and extract DNA barcodes from chromosomal DNA. Oligos used included 6-nucleotide multiplex indices specific for each experiment. Oligos contained regions homologous to barcode-flanking regions and also included adapter sequences to bind to the flow cell of the Illumina HiSeq 2000/2500 Equal amounts of PCR amplified barcodes were pooled for DNA sequencing (single-end read) using the Illumina HiSeq 2500 platform (Bauer Core Facility, Harvard Univsersity)