title: GSM8434756: Tgfbr1 MKO (flox/flox; LysMcre) fed MASH diet  for 30 weeks, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were isolated from frozen liver tissues using salt-Tris based protocol. Isolated nuclei were stained with propidium iodide before FACS sorting. Sorted nuclei were processed for snRNA sequencing. Total liver RNA was extracted from frozen tissues using RNA extract kit (Invitrogen, Cat: 12183025) Bulk RNA libraries were prepared using high-throughput sequenicng set following the user manual; snRNA libraries were prepared using standard Illumina and 10X genomics Chromium single cell guide