title: 16SrRNA gene amplicon sequencing of forrest soil microbiome
design: To analyze the microbial diversity and taxonomic composition of the samples, DNA extracts were sent to the IKMB sequencing facility (minimum 10k reads  per sample; Kiel University, Germany). Illumina MiSeq amplicon sequencing of the bacterial 16S rRNA gene was performed using primers targeting the V3-V4 region (V3F: 5-CCTACGGGAGGCAGCAG-3 V4R: 5-GGACTACHVGGGTWTCTAAT-3) (Caporaso et al., 2011).