title: GSM8217921: lumbar spinal cord, 393.37; Macaca nemestrina; RNA-Seq
library_construction_protocol: RNA was isolated from 100 mg frozen lumbar spinal cord samples using RNA-STAT60 reagent followed by cleaning with Promega RQ1 DNase set (M6101) and Qiagen RNeasy Mini Kit (74104). For the transcriptome sequence sample preparation, the NEBNext rRNA Depletion Kit (E6310) was used to purify isolated 1 ug RNA samples according to the manufacturer's recommendations. cDNA libraries were prepared with the NEBNext Ultra II Directional Library Prep with Sample Purification Beads (E7765) according to the manufacturer's recommendations. cDNA libraries were submitted to the Johns Hopkins University Genetic Resources Core Facility for sequencing.