title: GSM7798043: cardiomyocyte, DC2555, ATAC-seq; Homo sapiens; ATAC-seq
library_construction_protocol: DNA was tagmented using Illumina Tagment DNA TDE1 Enzyme and Buffer Kits (Illumina, #20034198) on permeablized cardiomyocytes (0.1% digitonin, Promega , #G9441) and then purified using DNA Clean and Concentrator (Zymo Research, #D4014). Libraries were prepared using Nextera DNA Library Prep Kit (Illumina, #15028211) complemented with Nextera Index Kit (Illumina, #15055289). Libraries were amplified using 11 cycles on the thermocycler and cleaned with Ampure XP Beads (Beckman Coulter, #A63881). Libraires were validated using Agilent High Sensitivity D1000 ScreenTape.