title: GSM7868203: Eosinophil-3T; Mus musculus; RNA-Seq
library_construction_protocol: Sorted eosinophils were centrifuged and RNA isolated with the Qiagen RNeasy RNA isolation kit per manufacturer's instructions. RNA quantity and quality were assessed using Qubit RNA quantification and electropherogram analysis. cDNA libraries were generated with the Illumina Stranded Total RNA Prep Ligation w/ RiboZero Plus kit. The libraries were multiplexed and 100bp paired-end sequencing performed in one lane of a NovaSeq 6000 SP flow cell using the Illumina XP workflow.