title: GSM7462250: HiC in ZNF274KO 1; Homo sapiens; Hi-C
library_construction_protocol: The Hi-C libraries were prepared using Arima-HiC (Arima Genomics), following the manufacturer's protocol. For all samples, 5 million cells were fixed in 1% methanol-free formaldehyde solution for 10min at RT and the reaction was quenched for 5min with glycine to a final concentration of 125 mM. UMI-4C experiments were performed as described previously (Schwartzman et al. 2016). Briefly, 5 million cells were fixed in 1% methanol-free formaldehyde solution for 10min at RT and the reaction was quenched for 5min with glycine to a final concentration of 125 mM. Cells were lysed in ice-cold Lysis Buffer (10mM Tris HCl pH 8,10mM NaCl, 0.2% NP-40), chromatin was digested with 800U DpnII (NEB), and digested ends were marked with biotin UMI- 4C (https://pubmed.ncbi.nlm.nih.gov/27376768/) is a 3D technique involving  primers and biotinilation/ligation to infer the contacts points of regions of interest. It's very similar to a 4C with the addition of UMI for deduplication.