title: Pore-C sequencing of adult, female desert hairy scorpion
design: 150 mg of ground tissue was utilized to crosslink cells, which were then exposed to permeabilization solution, denatured chromatin, and NlaIII enzyme digestion. Reversal of crosslinking occurred through suspension incubation with periodic rotation. Subsequently, 1.5 g of purified DNA was employed for library preparation using the SQK-LSK114 kit and protocol. DNA repair was carried out at 20 C for 20 minutes, followed by enzyme inactivation at 65 C for 10 minutes. Lastly, the PromethION flow cell was run for 96 hours.