title: GSM7745729: Heart, P1, B6, MI, Rep2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized and hearts were extensively perfused with cold PBS to removal peripheral cells. Infarcted myocardium was excised, minced with fine scissors, and digested with Collagenase Type II and DNase at 37°C for 30 minutes with gentle shaking. Larger pieces were broken down through vigorous pipetting and filtered through a 40 µm cell strainer. After RBC lysis, samples underwent dead cell removal (StemCell Technologies) by Annexin V staining. Next, samples underwent a CD45-positive selection (StemCell Technologies) to enrich cardiac leukocytes. Enriched cells and flow-through cells were both counted and recombined at a ratio of 2 CD45+ cells for every 1 non-CD45+ cell. Combined single cell suspensions were diluted to target a recovery of 5,000 cells per sample. The single cell RNA-sequencing libraries were prepared using the 10X Genomics Single Cell 3' Gene Expression (v3 or v3.1 chemistry) pipeline.