title: 16S-Seq of soil: Yixing, China
design: Microbial DNA was extracted using the HiPure Soil DNA Kits (Magen, Guangzhou, China).Amplicons were extracted from 2%agarose gels and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, U.S.) and quantified using ABI StepOnePlus Real-Time PCR System (Life Technologies, Foster City, USA). Purified amplicons were pooled in equimolar and paired-end sequenced (PE256) on an Illumina platform.