title: GSM6669722: Myocardial scar control_5; Mus musculus; RNA-Seq
library_construction_protocol: Infarcted heart tissue was dissected and stored in RNA later solution at 4°C overnight. Afterwards, samples were stored at -80°C until RNA extraction. RNA was extracted from mouse cardiac tissue (infarcted area) by using the tissue RNA isolation kit (RNeasy mini – Qiagen) according to the manufacturer's instructions RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for all samples was ≥7.5. DNA libraries suitable for sequencing were prepared from 50 ng of total RNA, with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer's instructions (½ volume). After 17 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~310 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips.