title: Sir3 EcoG2ID with nanopore sequencing in S.cerevisiae with sir2D or sir4D and NLSEcoG2ID with nanopore sequencing in S.cerevisiae with sir2D,sir3D or sir4D :saturated cultures
design: In vivo footprinting from a saturated culture using Sir3EcoG2 with a sir4 or sir2 deletion, or NLSEcoG2 with a sir2, sir3 or sir4 deletion and direct detection of Adenine methylation by nanopore sequencing (Oxford Nanopore). multi-read fast5 files basecalled with guppy basecaller and res_dna_r941_min_modbases-all-context_v001.cfg . Library strategy: Multiplexed library mix with the Rapid Barcoding Kit (RBK404: Sir3EcoG2sir2D;RBK405: Sir3EcoG2sir4D;RBK410: NLSEcoG2sir2D;RBK411: NLSEcoG2sir3D;RBK4112 NLSEcoG2sir4D).