title: GSM8170908: EU-seq of GFP-FKBP12F36V-Ctcf (Y226A/F228A) ESC mutant control, replicate2.; Mus musculus; OTHER
library_construction_protocol: Following 5-EU labelling, cells were quickly washed (1x) with PBS, and immediately lysed in RLT buffer and RNA was prepared using RNeasy Mini Kit (Qiagen). The labelled RNA was subsequently enriched using the Click-iT® Nascent RNA Capture Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. EU-seq libraries were prepared using the NEBNext Ultra kit (NEB) as per manufacturer's instructions and subsequently sequenced using a Next-Seq 500 Sequencer (Illumina).