title: =A25
design: The library was prepared using the NEBNext DNA Library Prep Kit following standard protocols. The DNA was fragmented, end-repaired, and A-tailed to facilitate adapter ligation. After ligation of sequencing adapters, the library was enriched through PCR amplification. Post-amplification, purification and size selection steps were performed to ensure the quality and consistency of the library. The final library was quantified and validated before being submitted for sequencing.