title: GSM8263753: Tumor, Prot_2.1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from FFPE tumor cores (n= 12 samples) using RNeasy FFPE kits according to the manufacturer's protocol (QIAGEN, Germantown, MD). RNA-seq libraries were generated using Truseq RNA Access Library Prep Kits (TruSeq RNA Exome kits; Illumina) and sequenced on Highseq3000 sequencers using 75 bp paired-end sequencing method (Illumina, San Diego, CA). For transcriptomic analyses, raw RNA-Seq count data were normalized for inter-gene/sample comparison using FPKM, followed by log2(x + 1) transformation, as implemented in the edgeR R/Bioconductor package