title: GSM7796944: HiC, lens epithelium, replicate 1; Mus musculus; Hi-C
library_construction_protocol: HiC: Lenses were dissected at P0.5 and then micro-dissected into lens epithelium and lens fiber under a dissection microscope. Each replicate of lens tissue was comprised of either 30 lens epithelium, or 30 lens fiber samples. Samples were kept on dry ice for the duration of the micro-dissection process. Samples were then homogenized using a disposable pestle tissue grinder. Homogenized tissues were fixed in 2.0% formaldehyde for 10 mins at room temperature. Formaldehyde was quenched with 0.125 M glycine to stop the reaction. The crosslinking method for mESCs was the same. ChIP-seq: 200 P0.5 lenses were obtained from CD-1 mice, microdissected into lens epithelium and lens fibers and stored in liquid nitrogen prior the use as we described earlier (Sun et al. 2015). The Hi-C library was generated using the Arima-HiC kit according to the manufacturers protocols (A510008) and performed by the NYU Langone Health Genome Technology Center. The ChIP-seq library was generated by Active Motif: Immunoprecipitation was performed on 6 µg chromatin from microdissected lens cells with 5 µl anti-CTCF antibody (ActiveMotif cat. # 61311, lot #11219006).