title: mock scRNA-seq using PB10X - gene expression with unblocked TSO  of Homo Sapiens: Loucy cells
design: The PB10X protocol for cDNA generation is described in detail in the paper. In this optimization experiment, 10 pg of priorly extracted RNA from the Loucy cell line was used per well to mimic the amount of RNA that can be expected from a single cell. In this experiment, a single unblocked TSO was used for all 96 wells. Library construction of the obtained cDNA was performed using 10X Single Cell 5 GEX Dual Index Library construction protocol, followed by paired-end Illumina NovaSeq 6000 sequencing, with read 1 28 nucleotides and read 2 90 nucleotides.