title: RNA-Seq of Lilium
design: High-quality RNA was enriched by Oligo (dT) beads. The enriched mRNA was fragmented into short fragments and reverse transcripted into cDNA with random primers. The cDNA fragments were purified with QiaQuick PCR extraction kit, end-repaired and ligated to Illumina sequencing adapters. The ligation products were selected according to their size by agarose gel electrophoresis, and initially amplified by PCR. The PCR production was constructed into a cDNA library and sequenced