title: RNA-seq of TFAP2B-overexpressed (Lv_TFAP2B_1) in rat primary neurons
design: Total RNAs were extracted from control transfectants (vector)  and TFAP2B-overexpressed rat primary neurons by Invitrogen TRIzol Plus RNA extraction kit, and sequencing libraries were generated using NEBNext Ultra II RNA Library Prep Kit. PCR products were purified by AMPure XP system, library quality was determined by Agilent Bioanalyzer 2100 system, and then sequenced on an Illumina Novaseq platform 150 PE mode.