title: Deep Sequencing of Korean Jindo Dog Reveals Evolutionary Trajectory of Coat Color Variations
design: Deep Sequencing of Korean Jindo Dog Reveals Evolutionary Trajectory of Coat Color Variations
library_construction_protocol: For whole genome sequencing, whole blood samples were collected from 113 Jindo dogs. The experiment was ethically approved by the National Institute of Animal Science, Rural Development Administration (approval no: NIAS2018-275). Total DNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. DNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ DNA Sample Preparation Kit (Illumina, San Diego, CA, USA).