title: GSM6670711: MDA-MB-231, parental, ATAC, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were washed with PBS and re-suspended in lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Transposition reaction was applied using Nextera XT Index Kit (Illumina), followed by DNA purification using a MinElute PCR Purification Kit (QIAGEN). The transposed DNA fragments were then subjected to 5 cycles PCR for pre-amplification first, and then amplified by PCR for additional 5 cycles.The amplified libraries subjected to gel size selection to purify 200-500 bp fragments.Libraries were validated using Tapestation (Agilent) and quantified using Qubit Fluorometer (Invitrogen)