title: GSM8264555: Decidua, DT treated 3; Mus musculus; RNA-Seq
library_construction_protocol: Decidual tissues were homogenized and RNA extracted as described previously (12). Successful DNA removal was confirmed and RNA concentration was measured using a Nano-drop Spectrophotometer ND- 1000 (Thermo Fisher Scientific), and RNA integrity was assessed by RNA Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA) to ensure all RNA samples had an RNA integrity number of >7. To measure mRNA expression, total DNase-treated RNA was reversed transcribed into cDNA from 800 ng RNA primed with 50 ng random hexamers using SuperscriptIV Reverse Transcription kit (Life Technologies) according to the manufacturer's instruction. qPCR was performed using 20 ng cDNA and PCR primers Stranded polyA libraries prepared using Nugen Universal Plus mRNA-seq (Part No. 0508), protocol M01442 v2.