title: 16S rRNA-Seq of hunman: adult male kidney
design: The 16S RNA sequencing used extracted DNA as the template for PCR amplification of bacterial 16S rRNA genes. The V3-V4 variable regions of 16S rRNA genes were amplified with universal primers. PCR amplified products were visualized and purified. The final purified amplicons were quantified using the Qubit dsDNA Assay Kit. The DNA concentrations were adjusted for high-throughput sequencing. Sequencing was performed on Illumina NovaSeq 6000 platform, generating 250-bp paired-end reads. The raw data were acquired in FASTQ format.