title: GSM7469361: acute-treated control 5; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from flash-frozen hypothalamus using Zymo research quick RNA miniprep kit (cat # 11-328) according to the manufacturer's recommendations. Isolated RNA was sent for library construction and sequencing by Novogene Corporation Inc. RNA was poly-A selected using poly-T oligo-attached magnetic beads, fragmented, reverse transcribed using random hexamer primers followed by second strand cDNA synthesis using dTTP for non-directional library preparation. Samples underwent end repair, A-tailing, adapter ligated, size selected, amplified, and purified. Illumina libraries were quantified using Qubit and qPCR and analyzed for size distribution using a bioanalyzer.