title: GSM6929065: TXNIP-null in MB135 cells, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from 3X106 cells using the Zymo Research Quick RNA miniprep kit (Zymo Research; R1055). 500 ng of total RNA was used to capture mRNA and construct library using KAPA Stranded mRNA-Seq Kit (KAPA Biosystems; KK8420) according to the manufacturer's protocol. Briefly, after mRNA capturing and fragmentation, cDNA was synthesized. Sequencing adaptors were ligated to the cDNA fragments. The adaptor-ligated cDNA fragments were amplified with library PCR primers that contain barcodes for about 10 cycles.