title: MDA-MB-361
design: DNA was extracted from cells using the DNeasy Blood and Tissue Kit (Qiagen) according to manufacturers instructions. The Genomics and Epigenomics Shared Resource (GESR) at Georgetown University Medical Center performed the WGS. Paired-end, indexed libraries for human WGS were constructed from 1.0 g of gDNA using the TruSeq DNA PCR-Free Library Prep Kit (Illumina) according to the manufacturers instructions. Briefly, DNA was fragmented using a Covaris M220 Focused-ultrasonicator (Covaris) using settings 230 for a 350-bp insert size. Library quality was assessed with a BioAnalyzer 2100 using the High Sensitivity DNA kit (Agilent Technologies). The libraries were quantified using the Kapa Library Quantification Kit Illumina Platforms (Kapa Biosystems). The denatured and diluted libraries were sequenced on a NextSeq 550 System (Illumina) using v2.5 High Output 300 cycle kit with 1% PhiX to an average sequencing depth of 50x coverage.