title: GSM8217136: ATAC, Lung, Ext1_KO,rep2; Mus musculus; ATAC-seq
library_construction_protocol: Lung tissues were digested with 5 U/mL Dispase (Corning, 354235), 0.025 mg/mL DNase I (Roche, 10104159001) and 480 U/mL Collagenase type I (Thermo Fisher, 17100017) in PBS solution at 37 °C for 1 h. Lung tissues were then transferred to C-Tubes (Miltenyi,130-093-237) and were mechanically dissociated on a gentleMACS Dissociator (Miltenyi). Samples were passed through 70 μm strainer and were centrifuged at 300 g for 5 min. Samples were treated with 1X RBC lysis buffer (Biolegend, 420301) for 5 min on ice. Cells were then collected in FACS buffer (PBS containing 1mM EDTA, 25mM HEPES and 0.5% BSA) for downstream experiments. 50,000 cells were captured and nuclei were isolated using lysis buffer (10mM Tris-HCl, pH 7.4, 10mM NaCl, 3 mM MgCl2, 0.1% IGEPAL CA-630). Nuclei were subjected to a transposition reaction and library construction using  TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, TD502) and TruePrep® Index Kit V2 for Illumina (Vazyme, TD202).