title: GSM8301909: PDX2263_day5_sgNeg_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from 0.5~5 million cells using Direct-zol RNA Miniprep Plus kit (ZYMO) or RNeasy Mini Kit (Qiagen) with DNase I treatment in column. For MOLM-13 and MV4;11 cells, RNA-seq libraries were prepared using QuantSeq 3' mRNA-seq Library Prep Kit (Lexogen) with 0.5-2 µg of total RNA according to the manufacturer's protocol. For PDX-2263 cells, RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina. Library quality was checked by High Sensitivity Bioanalyzer (Agilent) and libraries with different barcodes were pooled and sequenced on the Nextseq 500 platform with 75 or 150 bp single-end reads.