title: GSM7796176: GEM08Tg; Mus musculus; RNA-Seq
library_construction_protocol: Animals were sacrificed and livers perfused with 0.9% NaCl buffer. Livers were collected, minced, digested with Collagenase and DNase, and subsequently passed through a 100μm filter. Hepatic lymphocytes were then purified by a 2-step Percoll gradient.For live/dead cell discrimination, the ZombieDyeNIR dye was used according to the manufacturer's guidelines. In collaboration with the DKFZ FACS core facility, a FACS Aria II machine and a FACS Aria FUSION machine were employed for sorting CD45+ cells. Single-cell 3' RNA (gene expression) libraries were generated according to the 'Chromium Single Cell 3' Reagents Kits User Guide (v3.1 Chemistry)' (#CG000204, 10x Genomics). Library construction was performed with Chromium Single Cell 3' Reagent Kits v3 (10x Genomics) following the manufacturer's protocol.