title: RNA-Seq of Clostridiodes difficile deltaerm deltaCD25890 replicate 2
design: RNA for RNA-Seq was extracted from two independent biological replicates of WT and CD25890 mutant C. difficile strains, after 10 h of growth in SM, using a RNeasy Kit (Qiagen). Contaminating genomic DNA was depleted by two DNase treatments (Promega), according to manufacturer's recommendations. DNAse-treated RNA (5 g) was mRNA enriched using a Ribo-Zero Magnetic Kit (Epicentre).