title: TUMOR
design: Total RNA was isolated using the TRIzol reagent (lnvitrogen, USA) and quantifiedusing the NanoDrop ND-1000. MRNA was purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) (NEB, Ipswich, MA). Libraries wereconstructed using the NEBNext Ultra lI RNA Library Prep Kit (NEB), quantified usingthe 2100 Bioanalyzer System (Agilent Technologies Inc.) and sequenced on IlluminaNovaSeg 6000 system (San Diego, CA).