title: Illumina NovaSeq 6000 paired end sequencing; RNA-seq of splenic B cells from Pbx1-CKO and Ctrl mice
design: RNA-seq of splenic B cells from Pbx1-CKO and Ctrl mice
library_construction_protocol: Splenic B cells from Pbx1-Ctrl and Pbx1-CKO mice stimulated with anti-IgM (10ug/mL) for 6h were harvested. Total RNA was extracted using TRIzol reagent (15596018, Thermo Fisher) Total RNA was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.