title: GSM6667346: HMC_GVC; Homo sapiens; RNA-Seq
library_construction_protocol: As total RNA was isolated using Trizol method , QC validation and total RNA sequencing were all performed by ebiogen (Seoul, Korea). For control and test RNAs, the construction of library was performed using QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Inc., Austria) according to the manufacturer's instructions. In brief, each 500ng total RNA were prepared and an oligo-dT primer containing an Illumina-compatible sequence at its 5' end was hybridized to the RNA and reverse transcription was performed.