title: ddRAD of Aricia agestis from UK: adult female
design: DNA was extracted from the head and thorax using a Qiagen DNeasy Blood and Tissue kit. DNA was eluted in EB buffer and quantified using a Qubit 2.0 fluorometer. ddRAD libraries were prepared following Patterson et al. 2012. Briefly, genomic DNA was digested with PstI and EcoRI restriction enzymes. 48 individual libraries were pooled and insert sizes of 350-400bp were selected for each pool. Each pool was sequenced on an Illumina sequencing lane.