title: Illumina DNA-Seq of Sophora flavescens
design: Genomic DNA from the young fresh leaves of the same plant were isolated using the same methods as for the Nanopore sequencing. To generate small fragments for sequencing, high-quality genomic DNA was randomly fragmented using a Covaris ultrasonicator (Covaris, USA). Illumina sequencing libraries were constructed using the Truseq nano DNA HT library preparation kit (Illumina, USA)        with targeted insertion size of 350 bp. Purified libraries were loaded and sequenced on the Illumina NovaSeq 6000 platform (Illumina, USA).