title: GSM8432666: Ser3_cladeII_ND_time20_replicate1; Streptococcus pneumoniae; RNA-Seq
library_construction_protocol: Cultures were then centrifuged at 5000g for 10 mins. The pellet was resuspended in Buffer RLT and transferred to a MP biomedicals Lysing matrix B tube. Bacterial cells were disrupted in the TissueLyser (Qiagen) for 5 mins at 30Hz and supernatant was used as lysate input into the Qiagen RNeasy mini kit. Purified RNA was measured using Qubit and stored at -80℃. cDNA libraries were made using Illumina stranded RNA library preparation kits with RiboZero Plus rRNA depletion. Libraries were sequenced on NextSeq 550 to a target depth of 12M paired end reads (2x50bp) per sample.