title: GSM6764993: CEN.PK113-7D mtRNA Glucose replicate 2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNA was extracted using the miRNeasy Mini kit (Qiagen) with on-column DNA digestion Samples were poly(A) tailed enzymatically using using E. coli Poly(A) Polymerase (New England Biolabs #M0276), then purified using the miRNeasy Mini kit (Qiagen). Poly(A)-tailed RNA samples were used for library preparation using the Direct RNA Sequencing Kit (SQK-RNA002, Oxford Nanopore Technologies [ONT], Oxford, UK), following manufacturer's instructions.