title: GSM8619225: 22RV1 PDCD4-S23D1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Mini kit (Qiagen)) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification