title: GSM6761460: HAP1,ATO biol rep1; Homo sapiens; OTHER
library_construction_protocol: the genomic DNA was extracted by the TIANamp Genomic DNA Kit (#DP304, TIANGEN). A total amount of 1μg DNA per sample was used as input material for the DNA sample preparations. Sequencing libraries were generated using NEB Next® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's recommendationsandindexcodeswereaddedtoattributesequencestoeachsample. Briefly， the DNA samples were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing.After the DNA products were purified by AMPure XP system (Beckman Coulter, Beverly, USA), DNA concentration was measured by Qubit®3.0 Flurometer (Invitrogen, USA), libraries were analyzed for size distribution by Agilent 2100 Bioanalyzer and quantified by real-time PCR (>2 nM