title: RNA-seq of Polygonatum Odoratum rhizome
design: 1 g of total RNA of the rhizome of two cultivars was prepared for cDNA synthesis using an NEBNextUltra II RNA Library Prep Kit (New England Biolabs Inc., Ipswich, MA, USA) according to previously published protocol (Zhou et al., 2022). The quantity and purity of the library were checked using a Qubit 2.0 Fluorometer (Life Technologies, CA, USA) and a Bioanalyzer 2100 system (Agilent Technologies, Santa Clara, CA, USA). Finally, the RNA sequencing was performed using an Illumina NovaSeq 6000 platform (Illumina) by Shanghai OE Biotech Co., Ltd. (Shanghai, China).