title: 16S-V4 DNA-Seq of Homo sapiens: adult female noninflamed mucosa of terminal ileum
design: Genomic DNA was extracted with a QIAamp R DNA mini kit according to the manufacturers instructions. 30 ng of qualified genomic DNA samples and their corresponding fusion primers were selected for PCR, and parameters were set for amplification. Furthermore, Agencourt AMPure XP magnetic beads were used to purify the PCR amplification products and subsequently dissolve them in elution buffer, which was labeled to complete the database construction. An Agilent 2100 BioAnalyzer was used to detect the range and concentration of fragments in the library. The HiSeq platform was selected for sequencing of qualified libraries according to the size of the inserted fragments.