title: GSM7869274: culture_ChIP_MeCP2_KD_TOP2b_A300-950A_IP_2; Mus musculus; ChIP-Seq
library_construction_protocol: At 12 days in vitro (DIV12), primary neuron cultures were fixed 1% formaldehyde for 10 minutes. After quenching and cell lysis, to fragment DNA, the lysates were sonicated with Covaris E220 sonicator (5% Duty Factory, 140 Peak Incidence Power, 200 cycles per burst, milliTUBE 1mL AFA Fiber). ChIP was performed with H3K27ac (Abcam ab4729) and TOP2B (Fortis Life Sciences, A300-950A). ChIP libraries for H3K27ac were generated using Ovation Ultralow Library System V2 (NuGEN) and for TOP2B  using Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences). Libraries were pooled to a final concentration of 4-10nM and reads were generated on the Illumina Nextseq 500 with the Center for Genome Science at Washington University in St. Louis, typically yielding 15-40 million single-end reads per sample.