title: GSM8263449: Spleen_Fed [RNA-Seq]; Mus musculus; RNA-Seq
library_construction_protocol: Tissue samples from the same experimental group were pooled equally and approximately 15-40 mg of pooled frozen tissue powder was homogenized 20-25 times in a Dounce homogenizer with 1.2 ml of Trizol reagent (Invitrogen, Catalog No. 15596018) on ice. The RNAs that have been extracted by Trizol were purified using the MagMAX RNA extraction kit (Thermo Fisher Scientific, Cat.AM1830). RNA libraries were prepared for sequencing using standard Illumina kit and protocols; strand-specific