title: Whole genome sequence (Nanopore) of Burkholderia: PCR-C_Y9
design: First, large fragments of DNA were recovered by Blue Pippin automatic nucleic acid fragment recovery system, and then repaired. Barcode was added by PCR-free method of EXP-NBD104 kit of Oxford Nanopore Technologies Company. Afterwards, the size of fragments was detected by AATI automatic capillary electrophoresis instrument, and the samples were isomolar mixed. Finally, the SQK-LSK109 connection kit was used to link the adapter, and the 10K library was constructed.