title: GSM7528447: Mature leaf, H3K4me3, ChIP; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: anti-H3ac (06-599, Millipore) and anti-H3K4me3 (07-473, Milipore) antibodies were used to expract histone-DNA complex based on the phenol/chloroform/isoamyl alcohol-isopropanol DNA purification method Sequencing libraries were prepared according to manufacturere's instructions of TruSeq ChIP sample Preparation (Illumina). 10 ng of ChIPed DNAs were end-repaired to create 5' phosphoryated, blunt-ended dsDNA molecules. Following end-repair, these DNA fragments were subjected to addition of a single 'A' base, and TruSeq DNA UD indexing adapters were ligated. The products were then purified and enriched through PCR to create the final library. The libraries were quantified using the TapeStation D1000 Screen Tape (Agilent Technologies)