title: GSM8443054: HLM140_2, scRNAseq of 1 sample; Homo sapiens; RNA-Seq
library_construction_protocol: The PBMCs were thawed and fluorescence-activated cell sorting (FACS) was performed to isolate live CD3+TCRαβ-TCRγδ+ cells and live CD3+TCRαβ+TCRγδ- cells. These sorted T cells were then used to generate single-cell RNA sequencing (scRNAseq) libraries with the Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 (for infants) and v2 (for 5-11-year-old children), following the manufacturer's protocol (10x Genomics).