title: Nanopore DNA-Seq of Sophora flavescens
design: Young fresh leaves were collected and immediately used for high-quality genomic DNA isolation with the CTAB (cetyltrimethylammonium bromide) method. The quality of isolated genomic DNAs was examined using agarose gel electrophoresis, and then high-quality genomic DNA was randomly fragmented using a Megaruptor (Diagenode, NJ, USA). High molecular weight (HMW) DNA fragments were selected using the BluePippin system (Sage Science, USA), and then prepared and ligated with adapters using Nanopore SQK-LSK109 (Oxford Nanopore technologies, USA). Ligated DNA libraries were examined again using a Qubit and loaded on to Nanopore Flow cells R9.4, and sequenced on the PromethION platform (Oxford Nanopore technologies, USA).