title: GSM8301061: BMDM, LPS-bead positive, replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation was performed using TRIzol (Invitrogen), two sequential chloroform extractions, Glycoblue carrier (Thermo Fisher), isopropanol precipitation, and washes with 75% ethanol. RNA was quantified with the Bioanalyzer RNA 6000 Pico Kit (Agilent) cDNA libraries were constructed and amplified using the SMART-Seq v4 Ultra Low Input RNA kit (TaKaRa) per the manufacturers instructions. Libraries were amplified and then sequenced on an IlluminaNovaSeq 6000 (150bp paired-end).