title: GSM6928364: HCLOs, PCO, EP300; Homo sapiens; ChIP-Seq
library_construction_protocol: Organoids were fixed with 1% formaldehyde for 15 minutes. After quenching and cell lysis, chromatin was sonicated using a Bioruptor Pico sonication device (Diagenode). ChIP DNA was obtained by following the manufacturer's protocol for High-Sensitivity ChIP Kit, Abcam using ChIP Grade Anti-EP300/KAT3B antibody, Abcam. ChIP-seq libraries were prepared using the NEB Next Ultra II DNA Library Kit by Medgenome, Inc. 50 ng of DNA was provided as starting material for input and ip samples for sequencing. 40M (PE100) total reads per sample were generated for each sample.