title: GSM6928506: cWT,Pol II Ser5P,rep1; Mus musculus; ChIP-Seq
library_construction_protocol: approximately 100,000 sorted primary microglias each genotype were harvested and washed with PBS and once with wash buffe in the CUT&Tag Assay Kit ( Vazyme, TD903). The primary microglias are bound to Concanavalin A-coated magnetic beads, and the cell membrane is permeabilized by Digitonin. Genomic DNA regions of interest were isolated using  antibodies against  H3K27Ac (Active Motif, catalog number 39133，1:100)，H3K4me3 (Active Motif, catalog number39159,1:100), Poll (Active Motif, catalog number 39133，1:100), Poll ser5p (Active Motif, catalog number 39133，1:100), Pu.1 (Active Motif, catalog number39159,1:100), respectively. The enzyme pA-Tn5 Transposase precisely binds the DNA sequence near the target protein under the antibody guidance and results in factor-targeted tagmentation. DNA sequence is tagmented, with adapters (Vazyme, ,TD202) added at the same time at both ends, which can be enriched by PCR to form the sequencing-ready libraries.