title: WGS of Colletes collaris: adult male chest
design: After the sample was qualified, size-select of long DNA fragments were performed using the PippinHT system (Sage Science, USA). Next, the ends of DNA fragments were repaired, and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit. The adapter in the SQK-LSK109 (Oxford Nanopore Technologies, UK) was used for further ligation reaction and DNA liabrary was measured by Qubit 4.0 Fluorometer (Invitrogen, USA).