title: GSM8478283: put2.mock2.H3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated using the TRI Reagent® method (Sigma-Aldrich) following SAR assays. First, the lower (8, 9, and 10) leaves were inoculated with Pst (OD600=0.005) to induce local priming (SAR) or mock (MgCl2 10 mM, pH 7.0), and 24 hpi the uninoculated upper leaves (13, 14, and 15) were collected for RNA extraction. Then, 48 h after primary infection, upper leaves (13, 14, and 15) were systemically challenged with Pst (OD600=0.0005). These systemically challenged leaves were collected for RNA extraction at 24 hpi. Three biological replicates, each containing over 20 µg total RNA, were submitted to GENEWIZ® (https://www.genewiz.com/) for standard mRNA sequencing (by poly-A selection) using the Illumina HiSeq platform with a 2x150 bp configuration.