title: GSM7360614: Card9KO rep2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were euthanized with CO2 and immediately transcardially perfused with 20 ml 1X PBS. Brains were collected and their meninges and choroid plexus removed then the MACS-sorting protocol was used to isolate microglia as described (Norris et al., 2018). Tissues were homogenized by gently mashing through a 70 um cell strainer.  After centrifugation the cell pellet was resuspended in MACS buffer (Miltenyi Biotec, 130-0910376) to proceed with column purification of microglia using CD11b microbeads (Miltenyi, 130-093-634). We then performed sorting utilizing LS columns and a QuadroMACS magnet (Miltenyi, 130-042-401 and 130-091-051) according to manufacturer's instructions. Column-bound cells were analyzed for purity by flow cytometry and submitted for RNA-seq. RNA libraries were prepared for sequencing by GENEWIZ