title: GSM8125769: pLN-TLR stimulation; Mus musculus; RNA-Seq
library_construction_protocol: Mice were immunized subcutaneously (s.c.) in the rear footpads with or without 10 µg TLR stimulation in 40 µL PBS. Popliteal lymph node cells were collected 48 hrs after TLR stimulation  for cDCs (live MHCII+ CD11c+) FACS-sorting for scRNA-seq library preparation. The scRNA-seq libraries were constructed using the Chromium Single Cell 3ʹ Library, Gel Bead & Multiplex Kit (10x Genomics, V3.1) following the manufacturer's instructions.