title: 16S amplicon sequencing of Wt and Per01 fly guts: Blank8
design: DNA was extracted from samples using the DNEasy PowerSoil Pro Kit (Qiagen) according to the manufacturer's protocol. Barcoded PCR primers annealing to the V1-V3 region of the 16S rRNA gene (Fwd, 5-AGAGTTTGATCCTGGCTCAG-3; Rev, 5-ATTACCGCGGCTGCTGG- 3) were used for library generation. The resulting library was sequenced on the Illumina MiSeq using 2x300 bp chemistry.