title: GSM8648415: Hep3B cells, DMSO control, biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from cells or liver tissue using TRIzolTM (Life Technologies, Grand Island, USA) and purified using RNeasy kit (QIAGEN, Germantown, USA Cat#74106) according manufacturers instruction. For both cells and liver tissue, RNA-Sequencing RNA sample quality was first assessed using Agilent 2100 Bioanalyzer G2938C with the Aligent RNA6000 Nano Kit (Agilent; Santa Clara, CA, USA). Next, the NEBNext Poly(A) mRNA Isolation Module (NEB, Ipswich, MA, USA) was used for mRNA enrichment, followed by NEBNext® Ultra™ II Directional RNA library kit (NEB, Ipswich, MA, USA) library preparation. Cell libraries were sequenced on an Illumia HiSeq 1500 machine (HiSeq Rapid v2 flow cell, 50bp reads yielding 400M clusters). Mouse libraries were sequenced on an Illumina NextSeq 2000 machine (P2, 2x50 bp).