title: GSM8263900: Dura-LM-D7-2; Mus musculus; RNA-Seq
library_construction_protocol: The dura was dissected from the skull, then was minced into small pieces and digested with 1 mL of 1 mg/mL Collagenase VIII (#C2139, Sigma) and 0.1 mg/mL of DNase I (#DN25, Sigma) in 5%-FBS DMEM in a water bath at 37°C for 15 minutes. The enzymes were neutralized with 5 mL 5%-FBS DMEM, and samples were filtered through a 40 μm cell strainer, followed by centrifugation at 400×g for 5 minutes at 4°C. Sequencing libraries were conducted using the BD Rhapsody Single-Cell Analysis System (BD Biosciences), following the manufacture's protocol. Briefly, dura cells were loaded on BD Rhapsody Cartridge, followed by reverse transcription and exonuclease I digestion, library preparation, and sequencing on NovaSeq 6000 (Illumina) with 150-bp paired-end run.