title: Total RNA-seq of Saccharomyces cerevisiae: humanized hsh155 yeast DMSO incubation Lane 1 Replicate 1
design: After treatment, total RNA was extracted from cells. The quality of extracted RNA was assessed using High Sensitivity RNA TapeStation (Agilent). rRNA depletion was using the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit. After barcoding and amplification, libraries were then sequenced using the NovaSeq 6000 system (Illumina).