title: GSM7797972: AZ2456; Homo sapiens; RNA-Seq
library_construction_protocol: Tumors were disaggregated using the hard tissue homogenizing CK28-2 mL kit (#P000916-LYSKO-A, Bertin Technologies) following standard procedures in a Precellys® Evolution Homogeniser (Bertin Technologies). Total RNA from tumors was isolated using the RNeasey mini kit (#74104, Qiagen). Total RNA was quantified by Qubit® RNA BR Assay kit (Thermo Fisher Scientific) and the RNA integrity was estimated by using RNA 6000 Nano Bioanalyzer 2100 Assay (Agilent). RNASeq libraries were prepared with KAPA Stranded mRNA-Seq Illumina® Platforms Kit (Roche) following the manufacturer´s recommendations starting with 500 ng of total RNA as the input material. The library was quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay.