title: GSM8218477: Brain-Hippocampus, P15, WT, F, pup1; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were isolated from fresh frozen mouse hippocampal brain tissue using a sucrose gradient ultracentrifugation protocol. Nuclei were separated into 3 populations using FANS: NeuN+ nuclei, Olig2+ nuclei, and the double negative nuclei. Approximately 42000 nuclei per sample were loaded onto the Chromium single cell chips (10x Genomics), with each sample consisting of all double negative nuclei, supplemented with an equal amount of neuronal and oligodendrocyte-lineage nuclei up to 42000. This strategy has previously been used to enrich for less abundant CNS cell types (Gerrits et al., Acta Neuropathologica, 2021). Single-nucleus cDNA libraries were constructed and dual-indexed using the Chromium Single Cell 3′ Reagents Kit v3 and corresponding user guide (10× Genomics).