title: GSM8125772: PBMC-TLR stimulation; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood by using Ficoll (Solarbio, #P8900) gradient centrifugation. PBMC DCs were sorted as Lin- (CD3/CD19/CD14/) CD123- CD11C+ HLA-DR+ from freshly isolated PBMCs (purity>93%). The scRNA-seq libraries were constructed using the Chromium Single Cell 3ʹ Library, Gel Bead & Multiplex Kit (10x Genomics, V3.1) following the manufacturer's instructions.