title: Streptomyces brasiliscabiei causing potato common scab in china
design: After DNA sample passed the quality and quantity test, it was randomly interrupted by the Covaris ultrasonic crusher, the whole library was prepared by terminal repair, addition of a tail, addition of sequencing linker, fragment screening, PCR amplification and purification. Get the final DNA library. There are two methods to detect the library qulaity :(1) Aati was used to detect the integrity and insert size of DNA fragments.(2) QPCR was used to detect the effective concentration of the library.After passing the library test, the two-terminal sequencing was performed by pooling different libraries according to the requirements of effective concentration and target off-line data volume.After base calling and BCL2FASTQ transformation, the original sequences obtained by Illumina high-throughput sequencer are image files, and the FASTQ (short for FQ) format of the original sequences (sequenced reads) are obtained, they are called raw data or raw reads. The FASTQ format data contains the sequence information of the sequencing sequence (reads) and the corresponding sequencing quality information, where each reads consists of four lines.