title: 16S-Seq of mus musculus: heart failure at 5 days
design: We high-throughput sequenced the V3V4 region of the bacterial 16S rRNA gene using 341F and 806R primers. Polymerase chain reaction (PCR) amplifications, PCR product gel extractions, and sequencing library construction were performed according to a previous study. Sample libraries were finally sequenced at BIOZERON Biotech. Co., Ltd. (Shanghai, China) using the Illumina Novaseq6000 platform based on a 250 bp paired-end strategy.