title: GSM8127879: LNP-Ctrl, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were harvested 14 days lfollowing LNP-pE285K-mAb or LNP-Ctrl treatment, single cell suspensions were prepared and stained with APC-Cy7-conjugated anti-mouse CD45 antibody. Briefly, dead cells were excluded by staining with DAPI viability dye  and Fc receptors were blocked prior to the addition of PE-conjugated anti-mouse CD45  antibody. 1,0000 CD45+ live cells were sorted from each tumor sample using a FACSAria IIIu instrument. Equal numbers of sorted CD45+ cells from 5 mice per group were pooled, pelleted and resuspended concentrated by centrifugation, and resuspended at a density of 1 × 103 cells/μl in RPMI1640 medium containing 10% FBS. Approximately 10000 cells were allocated for the 10 × Genomics 5′ v 2 single-cell assay. TCR/BCR libraries were prepared using the Chromium™ single cell V(D)J enrichment kit (10× Genomics). Reverse transcription, cDNA amplification and library preparation were executed following the manufacturer's protocol. Approximately 10,000 cells were allocated for the 10 × Genomics 5′ v2 single-cell assay. TCR/BCR libraries were prepared using the Chromium™ single cell V(D)J enrichment kit (10× Genomics). Reverse transcription, cDNA amplification and library preparation were executed following the manufacturer's protocol.