title: Indo-Pacific king mackerel muscle HiFi
design: Libraries for single molecule real-time (SMRT) PacBio genome sequencing were constructed following the standard protocols of Pacific Biosciences company. Briefly, high molecule genomic DNA and RNA from several tissues were sheared to ~20 kb targeted size, followed by damage repair and end repair, blunt-end adaptor ligation, and size selection. Finally, the libraries were sequenced on the PacBio Sequel II platforms.