title: GSM8434573: tile5-abundance-control-rep1_merge_R1; Homo sapiens; synthetic construct; OTHER
library_construction_protocol: For both abundance and interaction assays and all conditions, plasmid DNA was extracted from 9 OD units of yeast cells using the ChargeSwitch Plasmid Yeast Mini kit (Invitrogen). Site saturation mutagensis libraries comprising MLH1 missense variants in the C-terminal domain of human MLH1 were prepared in each tile and cloned into pDONR221 by Twist Bioscience. Site saturation mutagensis in tiles