title: GSM8377850: P2_CFM_Day8_A1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were cultured for eight days. Total RNA was isolated from cell culture (n=3) usinginnuPREPDNA/RNA Mini Kit (AnalytikJena, Jena, Germany) as recommended by the manufacturer's protocol. RNA quality was determined with aBioDropDuo+ spectral photometer (Biochrom, Holliston, USA). PolyA-selected libraries were prepared from 200 ng of total RNA usingQuantSeq3′mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen), according to the manufacturer's instructions. Size distribution and quality of the libraries were assessedby fragment analyzer (Agilent) and final libraries were sequenced in 75 bp single-end mode on a NextSeq2000 with P3 chemistry.