title: GSM7868349: DRG neurons, MSMP depletion, replicate2; Mus musculus; RNA-Seq
library_construction_protocol: Muscle spindle macrophages (MSMP) were FACS-sorted from Pv:ChR2 mice after optogenetically activating proprioceptive neurons. L4-6 DRGs were freshly dissected from MSMP depletion mice and homogenised with homogenisation buffer. Neuronal nuclei from homogenised DRG were sorted by FACS. Sorted MSMP or DRG neuronal nuclei were collected in cold PBS and processed for 10x single-cell RNA sequencing according to 10X Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) protocol. The library of MSMP or DRG was sequenced by Illumina NovaSeq 6000 platform. read length 150bp