title: GSM7869285: culture_RNA_MeCP2_OE_7; Mus musculus; RNA-Seq
library_construction_protocol: At 12 days in vitro (DIV12), primary neuron cultures were harvested and total RNA was extracted using RLT buffer following RNeasy Mini Kit (Qiagen). RNA was rRNA depleted using NEBNext rRNA Depletion Kit (NEB). RNA libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit with NEBNext Mulitplex Oligos for Illumina (NEB) following manufacturer's protocols. Libraries were pooled to a final concentration of 4-10nM and reads were generated on the Illumina Nextseq 500 with the Center for Genome Science at Washington University in St. Louis, typically yielding 15-40 million single-end reads per sample.