title: scRNA-seq using PB10X - gene expression of Homo Sapiens: jurkat cells
design: The PB10X protocol for cDNA generation is described in detail in the paper. The PB10X-compatible cDNA libraries were further processed with the 10X Library Construction kit PN1000190 (10X Genomics) and the Dual Index TT Set A PN-1000215 (10X Genomics), following the 10X 5 Gene Expression (GEX) Dual Index Library construction protocol. The required volumes were halved while maintaining all prescribed concentrations as specified in the protocol. An input of 4-5 ng cDNA per pool was used. The final sample index PCR was carried out using 16 cycles of amplification. The PB10X libraries were sequenced across two AVITI sequencer runs, reading 26 nt for read 1, 90 nt for read 2, 10 nt for P7 index and 10 nt for P5 index.