title: RNA-seq of Euphorbia lateriflora stem tissue
design: cDNA library were prepared (by the Genomics lab in the Bioscience Technology Facility at the University of York) from 1 ug total RNA from each tissues using the NEBNext Ultra II Directional Library prep kit from Illumina in conjunction with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs), according to the manufacturers instructions. A 13-minute fragmentation time was used when eluting mRNA from polyA magnetic beads. Amplification of the final library involved 9 cycles of PCR using the NEBNext multiplex Oligos from Illumina (New England Biolabs). The sample was then run for paired-end 150 base sequencing on an Illumina NovaSeq 6000 instrument.