title: RNA-seq of Vibrio vulnificus: wild type_1
design: RNA purity was determined by assaying 1 microliter of total RNA extract on a NanoDrop One spectrophotometer. Total RNA integrity was checked using an Agilent Technologies 2100 Bioanalyzer with an RNA Integrity Number (RIN) value. mRNA sequencing libraries were prepared according to the manufacturers instructions (Illumina Truseq stranded mRNA library prep kit). RNA sequencing was performed on the Illumina HiSeq 2500 platform using paired end 100bp sequencing.