title: RNAseq of Drosophila mojavensis adult aristae
design: To extract RNA from D. mojavensis aristae the tissue was homogenized in TRIzol (Invitrogen) and RNA was purified using RNeasy Micro Kit (Qiagen). RNA sample quality evaluation, library preparations and sequencing was carried out at the NUSeq Core facility, Northwestern University. RNA integrity and concentration was assessed by on-chip electrophoresis with the Bioanalyzer (Agilent). The SMART-seq V4 Ultra low input RNA kit (Takara Bio) was used to construct a cDNA library from total RNA and paired-end sequencing was carried out with the Illumina HiSeq2000 sequencing system.