title: GSM6670954: RNA-Seq dcl1-24/hyl1-2, 21 C, rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Seedlings (50-100 mg) were collected by flash freezing in liquid nitrogen and stored at −80 °C until processing. Samples were ground to a fine powder in liquid nitrogen, and RNA was extracted using a Quick- RNA MiniPrep kit with on-column DNase I digestion (Zymo Research, Irvine, CA) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first-strand cDNA was synthesized using random hexamer primers, followed by the second-strand cDNA synthesis.