title: GSM7797034: mouse choroid plexus from control mouse sacrificed at time 28; Mus musculus; RNA-Seq
library_construction_protocol: After exposure to constant darkness to verify absence of locomotor activity rhythms as a marker of SCN lesioning success, the mice of both groups (n = 12 per group) were returned to the original LD12:12 regime for one week to synchronize with the LD cycle. Subsequently, the lights were not switched on in the morning (assigned as circadian time, CT0), and mice remained in constant darkness for the following two days during which they were sacrificed every 4 h (from CT0 to CT44, 12 time points). ChP from the 4th ventricle was removed by pincers and immediately immersed into RNAlater. FoxJ1-ERT2-Cre+/-; Bmal1fl/fl (KO) mice were sacrificed at two time points; 7 h after the lights on for the day time point, i.e., Zeitgeber time (ZT)7 and 6 h after the lights off for the night time point, i.e., ZT18, at Taipei Medical University, Taipei, Taiwan. Whole brains were removed, frozen on dry ice, embedded in OCT compound (Sakura Finetek), and then cold-shipped to Prague. Upon arrival, they were sectioned on cryocut and ChP from the 4th ventricle was sampled by laser-capture microdissection. rRNA depletion, polyA RNA enrichment, library preparation using KAPA mRNA HyperPrep Kit (Roche, Switzerland; for WT samples) or Smarter Stranded totRNA-seq low input kit (Takara, Japan; for KO samples)