title: GSM8263541: BMDMs, Nudt21 KO4, 0hr; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of BMDMs from WT and Nudt21-cKO mice was extracted using TRIZOL (catalog no. 15596026, Invitrogen) and was used as input material for the RNA sample preparations. RNA purification, reverse transcription, library construction, and sequencing were performed by Novogene Co., Ltd. Paired-end RNA-Seq was performed to yield more than 250 million reads per sample, which allowed us to obtain not only transcript expression but also alternative PAS usage. Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.