title: ITS2 amplicon sequencing Monterey Bay
design: PCR with ITS primer set (5.8SF- 5-TGCTTGTCTGAGTGTCTGTGGA-3; 28SR- 5-TATGCTTAAATTCAGCGGGT-3) designed to specifically target Pseudo-nitzschia ITS2; one-step PCR using the TruFi DNA Polymerase; purified using AMPure XP beads; quantified and pooled.