title: GSM6927779: LSD1_HCT116_KO; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted with a HiPure Tissue DNA Mini Kit (Magen, D3121-02). The DNA samples were digested using methylation-insensitive restriction enzyme Mspl. All the cytosines were subjected to methylation-modified sequencing adaptors, DNA fragments with insert lengths within the range of 40-220 base pairs were cut from the gel and bisulfite treatment was carried out using an EZ DNA Methylation Gold Kit (Zymo Research). PCR amplification was then performed to obtain the final DNA library. After quality checks of the DNA library, the samples were sent for Illumina HiSeq 2500/4000 sequencing.