title: Sequel II sequencing; Raw reads: L-TCD-01
design: SMRTbell libraries were generated according to the procedure & checklist 'Preparing whole genome and metagenome libraries using SMRTbell® prep kit 3.0', (102-166-600 REV02 MAR2023,  Pacific Biosciences) with the following modifications: genomic DNA was sheared using Speed 35 on the Megaruptor 3 (Diagenode) and an additional size selection with diluted AMPure PB beads (35% v/v) was performed on the SMRTbell library to remove fragments <5kb. The final libraries were sequenced on a Sequel IIe platform (Pacific Biosciences) using Sequel II Binding Kit 3.2, Sequencing Primer 3.2, Sequel II Sequencing Kit 2.0 and a 30hr movie time.