title: mock scRNA-seq using PB10X cDNA with ONT  of Homo Sapiens: Loucy cells
design: The PB10X protocol for cDNA generation is described in detail in the paper. In this optimization experiment, 10 pg of priorly extracted RNA from the Loucy cell line was used per well to mimic the amount of RNA that can be expected from a single cell. In this experiment, a single unblocked TSO was used for all 96 wells. The obtained cDNA was used as input for Oxford Nanopore Technologies PromethION sequencing, as stated in the SQK-NBD114.24  protocol using R10.4.1. flow cells. Basecalling was performed using MinKNOW 23.07.12 in superhigh-accuracy mode.