title: GSM7888507: wound _cornea; Mus musculus; RNA-Seq
library_construction_protocol: The eyes from 4 normal unwound (UW) mice and 4 mice 24 hours post-wound (W) were collected to extract corneas. The corneas of mice underwent an 18-hour digestion using dispase II (Roche) at 4°C to facilitate the separation of the epithelial layer from other components. Subsequently, the epithelial layer underwent trypsin digestion (Sigma-Aldrich), while the residual tissue was subjected to collagenase A digestion (Roche) at 37°C for a duration of 1 hour (Kawakita et al., 2004). Following this, the dissociated corneal cells from UW and W groups were severally combined and suspended anew, in preparation for 10x Genomic sequencing. Library generation was performed following the Chromium Single Cell 3' Reagents Kits v3 where we target 10,000 cellls per sample for capture.