title: The raw data of chloroplast genome sequence of Codonopsis pilosula (Franch.) Nannf.
design: A total amount of 0.2 g DNA per sample was used as input material for the DNA librarypreparations. Briefly, genomic DNA sample was fragmented by sonication to a size of 350bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for sequencing, followed by further PCR amplification. After PCR products were purified by AMPure XP system (Beverly, USA). Subsequently, library quality was assessed on the Agilent 5400 systemAgilent, USA and quantified by QPCR (1.5 nM). After library quality control, different libraries were pooled based on the effective concentration and targeted data amount. 5'-end of each library was phosphorylated and cyclized. Subsequently, loop amplification was performed to generate DNA nanoballs. These DNA nanoballs were finally loaded into flowcell with DNBSEQ-T7 for sequencing in Beijing Biomics Tech Co., Ltd (Beijing, China).