title: CRISPR screen:mus musculus: H22 xenografts
design: The mouse GeCKOv2 library, used in this study contains 130,209 sgRNAs targeting 21,786 genes. The stable Cas9-expressing H22 cell line was transduced with mouse GeCKOv2 library, which contains 130,209 sgRNAs targeting 21,786 genes, and injected subcutaneously into mice, BALB/c or BALB/c/Nude, which were then treated with vehicle and sorafenib for 14 days. After treatment, tumors were harvested and genomic DNA from whole tumor tissue was isolated. Sequences of sgRNAs were amplified by PCR, and the amplicons were sequenced using next-generation sequencing. Significantly depleted sgRNAs were identified using the MAGeCK algorithm.