title: MeRIP-seq of MCAO/R(2M06_T_ip) model in rat
design: Total RNA was isolated from  brain and subjected to RiboMinus treatment to remove rRNAs. IP RNA was then subjected to 2 rounds of m6A RNA immunoprecipitation (MeRIP) using an antibody that recognizes m6A. Then Input RNA and IP RNA sequencing libraries were generated using NEBNext Ultra II RNA Library Prep Kit, respectively. PCR products were purified by AMPure XP system, library quality was determined by Agilent Bioanalyzer 2100 system, and then sequenced on an Illumina Novaseq platform 150 PE mode.