title: Trimmed_RNA_seq_for_BW25113_sample_Replicate_4_Medium
design: RNA extractions performed on cells sorted into Qiagen RNAprotect. Enzymatic lysis and proteinase K digestion of bacteria performed following protocol 4 in RNAprotect handbook. Purification of total RNA from bacterial lysate using Qiagen RNeasy Mini Kit following protocol 7. DNase digestion using the Qiagen RNase-Free DNase set. rRNA depletion performed with Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit.