title: GSM7115834: anti-PD1_Rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA from bulk tumors, MACS-sorted tumor cells, and cultured cells were purified using a GenElute total RNA purification kit (Sigma-Aldrich). PolyA RNA was isolated using NEBNext® Poly(A) mRNA Magnetic Isolation Module, and bar-coded libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). Libraries were pooled and sequenced from a single-end (1X75) on an Illumina NextSeq 500 system using the High output V2 kit (Illumina Inc., San Diego, CA) at a sequencing depth of 23-31 million reads.