title: GSM6761422: 4sU-seq_HepG2_JTE-607_2; Homo sapiens; OTHER
library_construction_protocol: (1). MPIVA: RNA from in vitro assay is phenol chloroform extracted and polyadenylated RNA is purified using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). (2). 4sU-seq and (3). PAS-seq: RNA is extracted by Trizol. For 4sU-seq, RNA is biotinylated and 4sU labeled RNA is enriched by strepavdin beads and further purified by phenol chloroform extraction (1). MPIVA: Library is constructed by RT-PCR. (2). 4sU-seq and (3). PAS-seq: RNA is fragmented and library is constructed following illumina's protocol