title: GSM8170493: ATAC sequencing, BCPAP, 5-Azacytidine treated, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: genomic DNA was extracted from the collected tissue samples using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). as outlined in the manufacturer's recommendations and guideline. After DNA extraction, the DNA concentration and purity were assessed using Nanodrop (Thermo Fisher, Carlsbad, CA, US). ATAC libraries of BCPAP were prepared as done in previous study. In brief, the procedure is as follows. Cells are lysed following preparation guidelines, with fresh ATAC-seq lysis and wash buffers. Cells are pelleted and supernatant removed before resuspension in lysis buffer. After incubation, samples are diluted, washed, and pelleted again. The transposition mix is applied, followed by incubation. Post-incubation, DNA is cleaned and concentrated. Barcoding involves assigning unique adapters, PCR amplification, and cleanup. Library concentrations are quantified for sequencing. Then, the libraries were sequenced on a HiSeq2500 sequencer (Illumina, San Diego, CA, USA) with 2X 100bp paired-end reads featuring unique dual index, generating 2Gb of sequencing data from each sample.