title: GSM8264546: LSK cells, Control mouse; Mus musculus; OTHER
library_construction_protocol: Cells were captured with Concanavalin A beads and incubated with anti-H3K27me3 antibody (# 9733, Cell Signaling Technology) at 4°C overnight. Mouse Anti-rabbit IgG secondary antibody was added the next day. Cells were then incubated with pA/G-Tnp for 1h and the DNA fragmentation was performed. Libraries were constructed using Hyperactive Universal CUT&Tag Assay Kit for Illumina (#TD903, Vazyme) and TruePrep Index Kit V2 for Illumina (#TD202, Vazyme) according to the manufacturer's protocol