title: GSM7367039: DMD-4V Day 15 post transduction n1; Homo sapiens; ATAC-seq
library_construction_protocol: The ATAC-seq libraries were generated using 50,000 cells from two technical replicates of 1 DMD sample. The samples were used as detailed in (Corces et al. 2017). In brief, nuclei were isolated and were incubated with a transposase solution (with a final concentration of transposase buffer 1X, 100 nM of transposase, 0.01% of digitonin, 0.1% of Tween-20 and PBS 0.33X) and incubated in a thermomixer with shaking at 1000 rpm for 30 min at 37°C. The mixture was cleaned using DNA Clean & Concentrator-TM-5 kit (Zymo #D4003). Transpose DNA was amplified by 12 cycles of PCR using SureSelectQXT Library Prep for WGS (Agilent #9684). The library quality and fragment size were quantified using an Agilent bioanalyzer 2100 before to be sequenced on Illumina HiSeq 4000 platform with paired-end sequencing.