title: 16S rRNA seq of anaerobic digesters: bioaugmentation (semi-cont)
design: Isolated DNA from the effluent of anaerobic digesters were amplified with primers 341F and 806R targeting the V3 -V4 hypervariable region of the 16S rRNA. The 16SrRNA library was prepared and the gene libraries (2x300bp) were sequenced on the Illumina Miseq sequencer.