title: FVB-018
design: Whole genome libraries were constructed using genomice DNA and the KAPA HyperPrep Kit (Roche Sequencing and Life Science) according to the manufacturers protocols, targeting an insert size of 400 base pairs.  Briefly, the protocol entails randomly shearing the DNA using the E220 Focused-ultrasonicator (Covaris), size selection, ligating Illumina specific barcoded adapters, and PCR amplification.  The quality and concentration of the libraries were assessed using the D5000 ScreenTape (Agilent Technologies) and Qubit dsDNA HS Assay (ThermoFisher), respectively, according to the manufacturers instructions. Libraries were sequenced 150 bp paired-end on an Illumina HiSeq X using the TruSeq SBS Kit v3-HS.