title: Menadione reduces the expression of virulence- and colonisation-associated genes in Helicobacter pylori
design: Menadione reduces the expression of virulence- and colonisation-associated genes in Helicobacter pylori
library_construction_protocol: Bacteria were harvested by centrifugation and the cell pellets were submerged in Trizol and shipped on dry ice to Macrogen Inc. (South Korea) for RNA extraction and sequencing. Samples were relabelled in a randomised order before sending to the sequencing provider, to avoid any potential for introduction of a technical batch effect based on which group (treated or untreated) the samples belonged to. Total RNA extraction was conducted by Macrogen Inc. (South Korea). Total RNA was quality checked to confirm that all were of acceptable RNA Integrity Number (RIN >7). Libraries were constructed using the Illumina TruSeq Stranded Total RNA (Illumina Inc, San Diego) protocol with ribosomal RNA depletion (Ribo-Zero).