title: GSM8619120: H1944.DMSO.rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Approximately 50,000 cells were lysed and transposition reaction was performed with the Tn5 Transposase at 37°C for 30 minutes. The reaction liquid was purified with Qiagen MinElute PCR Purification Kit. Five cycles of PCR with custom Nextera PCR primers74 were conducted using NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs). The additional number of PCR cycles was determined by qPCR of the partly amplified products. The PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter: A63881), using double size selection (left ratio: 1.4x, right ratio: 0.5x) according to the manufacturer's protocol.