title: eDNA of Nematode:Sediments
design: The DNA fragments are broken using an ultrasonic crusher and the fragmented DNA is end-repaired. Adenine (A) is added at the 3' end and a "Y" junction is attached to the end of the DNA fragments. The ligated product is purified using magnetic beads, the library template is enriched using PCR amplification, and finally, single-stranded DNA fragments are produced by denaturation with sodium hydroxide.