title: CRISPR screen of WT RPE-1 hTERT RNF4 KO (T12)
design: Cells were transduced with a custom DDR focused CRISPR guide RNA library using a lentiviral vector (MOI ~0.2) at >1000-fold library representation. Succesfully transduced cells were selected using Puromycin for 48 h (T0), and then split into three replicates (A,B,C) that were subsequently cultured independently and passaged every three days.