title: GSM8376269: Sirt7-/-_Pax5K198R_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from frozen cell pellets using the Maxwell RSC simplyRNA Tissue Kit mRNA was enriched using oligo dT beads and fragmented. First strand cDNA was synthesized using random primers, and second strand cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of the 3' adenylated cDNA fragments. U-labeled second-strand template was digested with UDG and amplified by PCR. After QC, library was circularized and amplified to make DNA nanoball before sequencing.