title: GSM8434021: reHMGA1-2HR-2; Homo sapiens; ATAC-seq
library_construction_protocol: Samples were further processed by Azenta. See below. ATAC-seq library preparation and sequencing reactions were conducted at Azenta Life Sciences (South Plainfield, NJ, USA). Live cell samples were thawed, washed, and treated with DNAse I (Life Tech, Cat. #EN0521) to remove genomic DNA contamination. Live cell samples were quantified and assessed for viability using a Countess Automated Cell Counter (ThermoFisher Scientific, Waltham, MA, USA). After cell lysis and cytosol removal, nuclei were treated with Tn5 enzyme (Illumina, Cat. #20034197) for 30 minutes at 37°C and purified with Minelute PCR Purification Kit (Qiagen, Cat. #28004) to produce tagmented DNA samples. Tagmented DNA was barcoded with Nextera Index Kit v2 (Illumina, Cat. #FC-131-2001) and amplified via PCR prior to a SPRI Bead cleanup to yield purified DNA libraries. The sequencing libraries were clustered on a flowcell. After clustering, the flowcell was loaded on the Illumina instrument according to manufacturer's instructions. The samples were sequenced using a 2x150bp Paired End (PE) configuration.