title: RNA-seq of Homo sapiens:Huh7-A2-Combo-3
design: Total RNA was extracted using TRIzol Reagent (Invitrogen) and assessed for quality with the Agilent 2100/2200 Bioanalyzer (Agilent Technologies) and NanoDrop (Thermo Fisher Scientific).  RNA libraries for RNA-seq were prepared for sequencing using standard illumina protocols.