title: GSM7868969: CSBm/m, Fem Rep1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were deeply anesthetized with isoflurane and then perfused with 1 × phosphate buffered saline (PBS) through the heart. Kidneys were removed and then washed with PBS solution. Kidney was cut in the middle and flash frozen with liquid nitrogen and stored at -80°C.  RNA from the kidney was isolated using RNeasy (Macherey-Nagel, UK) per manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, follwed by the second strand cDNA sythesis using either dUTP for directional or dTTP for non-directional library.