title: Amplicon sequencing of soybean rhizosphere soil after inoculation with rhizobia
design: PCRs for amplicon sequencing were conducted on soybean rhizosphere DNA with 16S rRNA primers (from Caporaso et  al., 2012) and rpoB (targetting alphaproteobacteria primers designed in Wardell G. thesis 2024) for a time series experiment under different rhizobia inoculation treatments with 5 plant replicates. Amplicons were cleaned, indexed and sequenced at the Centre for Genomics Research (University of Liverpool), on Illumina Miseq (2x250 bp) in two sequencing pools (m1 and m2). Raw Fastq files were trimmed for the presence of Illumina adapter sequences using Cutadapt v1.2.1. Singlet and paired reads are provided.