title: GSM8301372: Ctrl-1; Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA was extracted from the exosomes of M0 microglia(Ctrl) and M1 microglia(OGD-R) using miRNeasy Mini Kit, and the RNA molecules in a size range of 18-30 nt were enriched by polyacrylamide gel electrophoresis. After a quality inspection of total RNA, rRNA was removed using a Ribo zero rRNA removal kit (Ribobio, Guangzhou, China). Subsequently, RNase R was used to digest linear RNA, and the reverse transcription was performed to synthesize dsDNA. Next, the end repair was carried out, followed by the ligation of sequencing adapters. Finally, after the PCR amplification and library quality inspection, the library was constructed.. circular RNA-Seq