title: Omni-C Galeolaria caespitosa
design: A Dovetail Omni-C library was prepared from muscle tissue, using Dovetails Omni-C Kit and protocol. Chromatin was fixed in place in the nucleus using formaldehyde, before digestion with DNAse I. Chromatin ends were repaired and ligated to a biotinylated bridge adapter, followed by proximity ligation of adapter-containing ends and reversal of crosslinks. DNA was then purified and treated to remove biotin outside ligated fragments. The library was constructed using NEBNext Ultra enzymes and an Illumina-compatible adapter, with biotin-containing fragments isolated by streptavidin beads before PCR enrichment. The Omni-C library was sequenced on the Illumina HiSeqX platform (Cantata Bio, California, USA) to ~30x sequence coverage.