title: GSM7889342: FAPs_150; Mus musculus; RNA-Seq
library_construction_protocol: Muscle samples were isolated, minced and digested using Collagenase A for 45 min at 37 °C. Dispase II was added for 30 minutes to the muscle lysates. Muscles slurries were passed 10 times through a syringe and cell strainer and centrifugated. The pellet was re-suspended in Ammonium Chloride Potassium lysis buffer to eliminate red blood cells and centrifuged again. Cells were re-suspended in sorting buffer consisting of 100 µl Hank´s balanced salt solution (HBSS) containing 0.4% FBS. Fibro-adipogenic progenitors were isolated freshly using fluorescence-activated cell sorting (FACS) using the following surface marker profile: CD45-CD11b-CD31-SCA1+. For each single cell library, 4 independent chips using a commercial microfluidics platform (IFC, Fluidigm) were used with a capacity of capturing up to 96 individual cells per loaded chip. For each chip loading, cells were pooled from three mice (i.e. 4 pools per experimental group). Individual cell quality was checked as per manufacturers' recommendation. Successfully captured cells and corresponding cDNA samples of individuals cells were subsequently processed by Smart-Seq2 cDNA library preparation (Clontech) using the Nextera XT Indexing kit (Illumina). Quality control was performed using an Agilent Bioanalyzer DNA High Sensitivity Kit for electropherogram tracing.