title: GSM7467756: Msn2DBD_Gal4AD_lib_nativeloc_D04_2; Saccharomyces cerevisiae BY4741; RNA-Seq
library_construction_protocol: RNA-seq: Modified protocol of the nucleospin 96 RNA kit (Macherey-Nagel, Duren, Germany). Specifically, cell lysis was done in a 96 deep-well plate by adding 450 μl of lysis buffer containing 1 M sorbitol (Sigma-Aldrich), 100 mM EDTA, and 0.45 μl lyticase (10 IU/μl). The plate was incubated at 30°C for 30 minutes in order to break the cell wall and then centrifuged for 10 minutes at 2,500 rpm, and the supernatant was removed. From this point, extraction proceeded as in the protocol of nucleospin 96 RNA kit, only substituting β-mercaptoethanol with DTT 1M. RNA libraries were created as described before (Chapal et al., 2019).