title: GSM8217229: PCdNK_B (replicate 1); Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from dissociated tumor tissue using the RNAqueous Total RNA Isolation Kit (Ambion) Sequencing library generation, mRNA-seq and data analysis were performed by Novogene. A total amount of 2 μg RNA/sample was used as the input material. Messenger RNA was purified from total RNA using poly-A oligo-attached magnetic beads.  After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. End repair, A-tailing, adaptor ligation, size selection, amplification and purification were performed.