title: GSM8619182: H3A110C_SC1; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: RNA was extracted from whole yeast cells using hot acid phenol, followed by a further phenol-cholorform extraction and ethanol precipitation. Prior to preparing RNA-seq libraries for Illumina NovaSeq sequencing, contaminating DNA was digested using Turbo DNase (Thermo Fisher). RNA quality was assessed by Agilent 2200 Tapestation. Stranded mRNA (Poly-A) libraries were constructed with automated NGS library construction and sequenced by UCLA Technology Center for Genomics & Bioinformatics