title: 16S Endophytic F16WWS1MB  Roots Replicate1
design: 10g of roots were placed in 50mL Falcon tubes. Falcon tubes were filled with minimum medium, shaken 10min at max speed at 4C. After this, supernatant were discarded. Then, root surface was sterilized with serial washes in ethanol 70% during 3 min, followed by 5 min in NAClO 2.5% and five rinses with sterilized MiliQ water. DNA extraction was performed using 250 mg of root tissue homogenized by maceration in liquid nitrogen, using DNeasy PowerSoil Pro kit, following manufacturer`s instructions. Then the 16S rDNA V3-V4 region was amplified by PCR using 341F and 806R primers. Samples were sequenced using Illumina Novaseq PE 2x250bp reads.