title: DNA damage triggers activation of immune response to viral pathogens in plants
design: The cleaved RNA fragments are copied into first strand cDNA using reverse transcriptase and random primers. These cDNA fragments then have the addition of a single 'A' base and subsequent ligation of the adapter. The products are then purified and enriched with PCR amplification. We then quantified the PCR yield by Qubit and pooled samples together to make a single strand DNA circle (ssDNA circle), which gave the final library.