title: GSM8618722: Input.SJ.BM.9.5Gy.2; Mus musculus; ChIP-Seq
library_construction_protocol: Briefly, approximately 20x10^6 cells were crosslinked with 1% formaldehyde solution, quenched with glycine (0.125 M) and harvested in cold PBS. Sonication of crosslinked chromatin was performed using a Bioruptor® UCD-200TM Sonicator (Diagenode) with optimized cycles for each cell type. Immunoprecipitation of sonicated material was performed with antibodies against VDR (D2K6W) – Cell Signaling) or IgG (Rabbit IgG sc2729 – Santa Cruz) for 16 hours, and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed, reverse crosslinked, and treated sequentially with RNase and proteinase K prior to DNA isolation. Sequencing libraries prepared from immunoprecipitated DNA. ChIP-Seq libraries were prepared using Illumina's TruSeq Stranded protocol.