title: 16S of rhizospheric soil of mexican lime trees overexpressing CsPP16-lysozyme and CsPP16-defensin
design: The study was conducted in genetically modified C. aurantifolia trees expressing human lysozyme C and human -defensin-2, with composite soil samples collected and DNA extracted from rhizospheric soil using the PowerSoil DNA Isolation Kit, followed by amplification of barcoded amplicons of the V3-V4 variable region of 16S rRNA through PCR and subsequent library construction and high-throughput sequencing using the Ion Torrent PGM System.