title: Genomic DNA, PacBio SMRT sequencing long reads, Bradysia coprophila adult X'X female-producing female heads, Edinburgh
design: Genomic DNA was extracted using a version of the Qiagen DNeasy Blood and Tissue Kit extraction protocol modified for high-molecular weight (HMW) extractions and quantified using the Qubit (ThermoFisher). DNA from 30-40 pooled X'X heads was used for sequencing on the PacBio Single Molecule Real Time (SMRT) sequencing Sequel platform for long reads.