title: GSM6928823: SJINF001-D ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: Following FACS, 50,000 leukemic cells were immediately processed for ATACseq by the NYU Genome Technology Center. ATAC libraries were generated based on the protocol by Buenrostro et al. Briefly, cells are resuspended in cold lysis buffer (10 mM Tris Cl, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630, pH 7.4) and centrifuged for 1 minuted at 500xg. with one modification. Nuclei were tagmented using Nextera (Illumina) Tagmentation DNA buffer and enzyme.