title: Transcriptome of Dro-cytosolic-0-4h
design: The method to purify poly-adenylated RNA of ribosome-associated RNA was optimized using the RiboMinus Eukaryote Kit for RNA-Seq (#A10837-08, Ambion) to delete ribosomal RNAs (rRNAs) and Dynabeads oligo (dT)25 (#61002, Life Technologies) purification to select RNAs with poly-adenylated tails. The strand-specific RNA-seq library of the ribosome-associated poly-adenylated RNA was prepared using the Illumina TruSeq Stranded mRNA Sample Preparation Kit (A10837-08, Ambion).