title: RNA-Seq of synthetic infant gut consortium: bifidobacterium bifidum removed
design: The pellet from 1 ml of each sampled hour culture was resuspended in 2 ml RNAsafe solution and astored at -80 until RNA extraction. RNA was extracted with 1 ml RLT buffer from the RNeasy Kit (QIAGEN) containing 1% -mercaptoethanol (added prior to the use of buffer). The suspension was centrifuged to separate the aqueous phase from the organic phase. The aqueous layer was precipitated in ethanol, and RNA was redissolved in 400 l RLT buffer and further purified using RNeasy columns (QIAGEN) according to the manufacturer's instructions. The size distribution and the quantity of the isolated total RNA samples were determined using an Agilent 2100 bioanalyzer (Agilent) high-resolution electrophoresis system