title: GSM8619527: root, rep1; Populus trichocarpa; RNA-Seq
library_construction_protocol: Single-nucleus RNA-sequencing (snRNA-Seq) was conducted on nuclei isolated from pioneer root fragments corresponding to the primary stages of vascular tissue development. The method for nuclei isolation was adapted from Dorrity et al. (2021) with minor modifications for Populus roots. Single-nucleus transcriptomic libraries were prepared with the Chromium Controller and the Chromium Next GEM Single Cell 3′ Reagent Kit (10x Genomics, USA), following the manufacturer's instructions. Approximately 20,000 nuclei were combined with reverse transcription reagents and processed on the Chromium Next GEM Chip G to produce gel beads in emulsion (GEMs). This process released primers from the gel beads, facilitating the synthesis of barcoded cDNA from captured mRNA.