title: GSM7745946: CRISPR-seq-ESC-1; Mus musculus; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted using the phenol/chloroform/isoamyl alcohol (25:24:1 ratio) method. Genomic DNA was subjected to DNA bisulfite conversion using the Methylation-GoldTM Kit (ZYMO RESEARCH, #D5005). DNA were then used for PCR amplification using the NEB Taq DNA polymerase (NEB, #M0273S). The PCR products were then purified by the QIAquick PCR purification kit (Qiagen, #28104). Paired-end Tru-Seq compatible Illumina libraries were prepared by the Center for Computational and Integrative Biology (CCIB) DNA Core at Massachusetts General Hospital. Briefly, partial Illumina compatible adapters with unique barcodes were ligated onto each sample during library construction. Subsequently, a low-cycle PCR amplification step completeed the addition of the full-length adapter sequences.