title: Verification of B. anserina BA2 F27 plasmid topology and size by enzymatic digestion and ONT adaptive smapling sequencing
design: Genomic DNA was treated with different enzymes to determine the topology and size of the F27 plasmids. The DNA was sequenced with a MinION Mk1b instrument using an R10.4.1 flow cell with the SQK-NBD114.24 library preparation kit. Two libraries were prepard for B. coriaceae Co53 to generate enough reads to analyze. They are specified as lib1 and lib3. MinKNOW v22.12.7 (lib1) and v23.04.3 (lib3)  were used to collect the data with the adaptive sampling enrich function enabled with B. coriaceae Co53s  F27 plasmid RefSeq sequence provided in FASTA format (NZ_CP075082.1).  The data were basecalled with Guppy v6.5.7 using the HAC model, a Q score filter of 10 and the --detect_mid_strand_adapter options. The samples were demultiplexed using the same version of Guppy with the --detect_mid_strand_barcodes option. Four fastq files are provided for each library (lib1 and lib3), 1) reads from genomic DNA that was not treated with enzyme, 2) reads from genomic DNA treated with S1 nuclease only, 3) reads from genomic DNA treated with the EcoRV restriction enzyme, and 4) reads from genomic DNA treated with S1 nuclease and EcoRV enzyme.