title: GSM8263659: Drosophila testes, 32h PHS, total lysate, Replicate 1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: For Polysome profiling, RNA was isolated by acid phenol-chloroform extraction and fractions were combined based on the absorbance profiles at A260 nm to create 6 groups: free, 40S, 60S, 80S, 2-3 ribosomes and 4+ ribosomes RNA libraries for RNA-seq were prepared using QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) following manufacturer's protocols.