title: GSM7796983: Arabidopsis,overexpression ,three weeks; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: total RNA isolation using the E.Z.N.A Plant RNA Kit (R6827; Omega Bio-tek). All total RNA samples were treated with DNase I during extraction. RNA quality and concentration (>200 ng/μl) was examined by electrophoresis and cDNA from 50 ng total RNA was generated. Then 3 µg of the cDNA was fragmented, and 400 ng of the fragmented cDNA was used with an average size of 400 bp for library preparation (QiaQuick PCR extraction kit; QIAGEN). The libraries from each genotypes were sequenced on an illumine HiSeq 2000 (100 base paired end reads).