title: RNAseq of organ-cultured human hair follicles exposed to MEKi (Cobimetinib)
design: RNAseq of organ-cultured human hair follicles exposed to MEKi (Cobimetinib)
library_construction_protocol: Tissue samples from hair transplant surgeries were collected with written informed consent and ethics approval granted from UK North-West Haydock Research Ethics Committee (19/NW/0082). Isolated full-length anagen VI hair follicles were cultured 24hr as previously described (PMID: 26284830). Media was replaced every 48hrs with the following additions: Cobimetinib 1 μM (Selleckchem); or vehicle control DMSO (0.5%).   Total RNA was extracted from 6-8 full-length anagen VI hair follicles using the RNAeasy mini kit (Qiagen, Manchester, UK) according to manufacturer's protocol.  Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF).  Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.