title: Microbiome in human enamel biofilm with non treatment
design: Libraries were sequenced on the MiniSeq system (Illumina Inc, San Diego, CA, USA) considering a mean of 89,241 reads (2x300 pb) per sample. Sequencing output from the Illumina MiniSeq platform was converted to fastq format and demultiplexed using Illumina Bcl2Fastq 2.18.0.12. The resulting paired-end reads were processed using QIIME 2 2018.11. Index and linker primer sequences were trimmed using the QIIME 2 invocation of cutadapt. The resulting paired-end reads were processed with DADA2 through QIIME 2, including merging paired ends, quality filtering, error correction, and chimera detection. Amplicon sequencing units from DADA2 were assigned taxonomic identifiers with respect to Green Genes release 13_08 using the QIIME 2 q2-feature-classifier.