title: GSM6927784: 28.5_F2_Ctrl_Rad; Danio rerio; RNA-Seq
library_construction_protocol: Total mRNA was extracted from liver tissue of the zebrafish using Qiagen's miRNeasy Mini kit (Qiagen, Hilden, Germany). To prepare mRNA seq kit (poly A capture, KAPA RNA hyper)(Illumina, San Diego, CA, USA);  was utilized; 100ng of total input muscle RNA was used in accordance with the manufacturer's protocol. High through-put RNA sequencing (RNAseq) was performed at the Queens University Belfast, Genomics Core Technology Unit on an Illumina Next SEQ 2000 instruments with the mRNA library sequenced to a minimum depth of 50 million reads using forward stranded  PE50 strategy.