title: GSM6927986: Male Aedes albopictus-1; Aedes albopictus; ncRNA-Seq
library_construction_protocol: total RNA  was extracted with Takara RNAiso Plus (Takara, Dalian, China) circRNA-seq library was prepared with approximately 1μg of total RNA using KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina® (Kapa Biosystems, Inc., Woburn, MA) and using RNase R (geneseed, Inc., Guangzhou, China) to digest linear RNA . Briefly, ribosomal RNA was removed from the total RNA. Next, the ribominus RNAs were fragmented and then first strand and directional second strand synthesis were performed. Then the A tailing and adapter ligation were performed with the purified cDNA. Finally, the purified, adapter-ligated DNA was amplified. The library quality and concentration was assessed by utilizing a DNA 1000 chip on an Agilent 2100 Bioanalyzer. HiSeq X10 PE150 circRNA-seq