title: GSM8218122: Ck0315_8; Mus musculus; RNA-Seq
library_construction_protocol: For samples 1-16, total RNAs were isolated using RNeasy Plus Mini Kit (Qiagen). 300 ng of total RNA was used for the construction of RNA-seq library. The quality of total RNA was detected using Qubit RNA IQ Assay Kits. Poly(A) mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB#E7490). The RNA-seq libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7760S) following manufacture's protocol. The library concentrations were meansured using Qubit 4 fluorometer. The fragment distributions of the libraries were detected using Agile 2100 Bioanalyzer.