title: GSM8377026: 20233_SetPoint_TCRgd; Homo sapiens; RNA-Seq
library_construction_protocol: 200 cells were directly sorted into 1x lysis buffer supplemented with 6U RNase inhibitor (both Takara Bio). Post sort, sample tubes were vortexed, incubated at RT for 2 minutes, and snap frozen on dry ice prior to storage at -80˚ C. Upon thawing, cDNA synthesis was performed using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) as per the manufacturer's instructions. A total of 150-300pg amplified cDNA was used as input for library preparation