title: GSM7796561: WT_Retina_LG_24mo_24; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from 100mg of liver tissue or whole retina using a RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.  RNA purity and concentration were assessed using a NanoDropTM spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA).  A 4200 TapeStation System (Agilent Technologies, Santa Clara, CA, USA) was used with RNA screentapes to generate RNA integrity numbers (RIN) for RNA samples. All RNA samples selected for RNA-seq library preparation had a RIN greater than 7. RNA-seq libraries were prepared using a TruSeq Stranded mRNA Sample Prep Kit (Illumina, San Diego, CA, USA)