title: Nanopore GridION sequencing of SARS-CoV-2 Nasopharyngeal sample isolate V06110 using Long-range PCR primers
design: Viral RNA was reverse transcribed using LunaScript RT SuperMix Kit to generate cDNA. cDNA amplification was carried out in two pools of long-range primers with amplicon size of 4500 nucleotides (PMID: 38440140) followed by library preparation using rapid barcoding kit SQK-RBK004 from Oxford Nanopore. Sequencing was carried out for 24 hours using MinKNOW in GridION.