title: HiC illumina DNA-Seq of Sophora flavescens
design: The Hi-C library was prepared using a modified method according to the protocol from Ramani et al.. In summary, young fresh leaves from the same plant were collected, and fixed using formaldehyde. Then fixed tissues were homogenised and centrifuged to isolate nuclei. Cross-linked chromatin was digested with DpnII and labelled with Biotin, and then was ligated using T4 DNA ligase. DNA was purified and examined using agarose gel electrophoresis. Finally, the library      was prepared and sequencing was carried out according to the above-mentioned Illumina sequencing protocol.