title: DNA-seq of wild bulk sweet sorghum
design: The library was prepared using 2025 ng of DNA, after which the samples were concurrently fragmented and an adapter sequence added. After that, the adapters were employed in a limited-cycle PCR to introduce unique indices into each sample. The final library concentrations were determined using the Qubit dsDNA HS Assay Kit (Life Technologies) after the libraries were prepared, and the average size of the library was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were pooled and diluted to 0.6 nM before being sequenced for 300 cycles paired-end on the NovaSeq system (Illumina) 6000 system.