title: GSM8217192: PD1-Resistant MM409; Homo sapiens; RNA-Seq
library_construction_protocol: 500 ng of total RNA was purified from tumor biopsies using Qiagen Rneasy Kit according to the manufacturer's instructions. The RNA-Seq libraries were prepared from the purified RNA by using TruSeq RNA Library Prep Kit v2 (Illumina) for gene expression analyses. Paired-end RNA-Seq was performed with HiSeq (Illumina). An average of ~40 million paired-end reads.