title: Tovar MPRA library cDNA rep 3 from 832/13 INS-1 cell line
design: For the second library, we electroporated 30 ug of plasmid into 30 million 832/13 cells. We lysed cells 24 hours later with Buffer RLT Plus and isolated total RNA with the Qiagen RNeasy Midi Kit. We reverse transcribed 30 ug of DNAse I-treated total RNA using SuperScript IV. We performed a cDNA amplification PCR and sample indexing with all cDNA made.