title: GSM7798045: cardiomyocyte, H3K4me1, CUT&RUN; Homo sapiens; OTHER
library_construction_protocol: Cells were prepared using the CUT&RUN Assay Kit (CST, #86652). CUT&RUN fragments were purified using Monarch PCR & DNA Cleanup Kit with oligonucleotide cleanup protocol (New England Biolabs, #T1030L). Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, #E7645L) complemented with NEBNext Multiplex Oligos for Illumina (New England Biolabs, #E6440L). End repair and A tailing were performed for 30 minutes at 20C and then 1 hour at 50C. DNA was cleaned using 0.9X Ampure XP Beads (Beckman Coulter, #A63881). Libraries were amplified using 13 cycles on the thermocycler and cleaned with 0.9X Ampure XP Beads (Beckman Coulter, #A63881). Libraires were validated using Agilent High Sensitivity D1000 ScreenTape.