title: GSM8619084: H1944.IFNAR1sg.MPS1i.J2.rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were collected in ice-cold PBS and lysed in RIP buffer with protease inhibitors. Ten percent of the lysate was used for total RNA Input, while the rest was used for dsRNA immunoprecipitation with the J2 antibody. RNA was purified using TRIZOL and the QIAGEN miRNeasy Mini Kit. RNA-seq libraries were prepared using SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian, and DNBSEQ MGIEasy Universal Library Conversion Kit according to the manufacturer's instruction.