title: P2-input
design: Total RNA from tissue samples was extracted using TRIzol reagent (Invitrogen). Then, RNA-seq and m6A-seq were performed simultaneously (Aksomics Inc., Shanghai, China). For RNA-seq, 1 g of total RNA from tissue was subjected to poly(A) mRNA isolation with Dynabeads Oligo-dT. A cDNA library was constructed based on the protocol for the TruSeq RNA Library Prep Kit v2 (Illumina). Ultimately, 2  150 bp paired-end (PE150) sequencing was performed on an Illumina NovaSeq 6000. For MeRIP-seq, polyadenylated RNAs was enriched from total RNA and was further fragmented into approximately 100-nucleotide-long oligonucleotides. Protein-A/G beads was incubated with anti-m6A affinity purified antibody for 30 minutes at room temperature. The mixture was then immunoprecipitated by incubating with fragmented mRNA at 4C for 2 hours. High-throughput m6A was performed on the Illumina NovaSeq 6000. KAPA Stranded RNA-seq Kit was used to construct m6A-modified mRNA and input RNA sequencing libraries separately.