title: Mitochondrial DNA sequencing of Homo sapiens: U3035 Glioblastoma Cell Line
design: Library source was DNA extracted from isolated mitochondria. The SparQ DNA Fragment and Library Prep kit was used to build the mitochondrial DNA libraries using 1 to 4 ng of template according to protocol. The completed libraries were quantified using a Qubit 4 Fluorometer and run on an Agilent Bioanalyzer with the High Sensitivity DNA chip to determine the average size of each library. The libraries were pooled together, at an equal molar ratio, and run on a Miseq with an Illumina V2 300 cycle (PE150bp) reagent kit.