title: GSM8170572: L3_Syp_IP_R1; Drosophila melanogaster; RIP-Seq
library_construction_protocol: Whole larval and larval head samples were frozen and ground into find powder in liquid nitrogen. These samples were crosslinked with four rounds of 0.2 J/cm2 UV light irradiation at 254 nm. Dissected brains were crosslinked with four rounds of 0.15 J/cm2 UV light in PBS. Lysates were prepared in iCLIP lysis buffer (Buchbender et al. 2020) supplemented with AEBSF and RVC. Libraries were constructed using iCLIP2 procotol (Buchbender et al. 2020)