title: GSM6670757: MDA-MB-231, SAP30-KO1, Input for Pol II, rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a ultrasonicator (Brandson). ChIP-seq libraries were prepared using the ThruPLEX DNA-seq kit (Takara). 1 ng of DNA was used as starting material for input and ip samples. Libraries were amplified 13 cycles on the thermocycler. Post amplification libraries were gel-purified for size selection at 300-500bp. Libraries were validated using Tapestation (Agilent) and quantified using Qubit Fluorometer (Invitrogen).