title: GSM7889103: Standard _secondary heat step_microchem; Homo sapiens; RNA-Seq
library_construction_protocol: Human iPSC-derived alveolar epithelial type II cells were dissociated using dispase and trypsin, and FACS-sorted on calcein blue-positive live cells using a Mo-Flo Astrios Cell Sorter. 10X Chromium System, with altered variables: addition of a secondary heat step during cDNA synthesis, and thawing of sample in secondary container of 5% MicroChem Plus prior to adding Recovery Agent