title: GSM8263583: 3C_Tko_smc_rep2; Thermococcus kodakarensis; OTHER
library_construction_protocol: Cells were crosslinked, permeabilized, and treated with AluI and HaeIII to digest genomic DNA. The insoluble fraction was collected and used for proximity ligation. After reversal of crosslinks, the DNA was extracted with phenol:chloroform:isoamyl alcohol, precipitated in ethanol, and sonicated. Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina with Sample Purification Beads.