title: RNA-seq of common vetch
design: Oligo(dT)-attached magnetic beads were used to purify mRNA. Purified mRNA  was fragmented into small pieces and  First-strand cDNA was generated using random hexamer-primed reverse transcription,  followed by a second-strand cDNA synthesis and purified using AMPure XP Beads.  Afterwards, A-Tailing Mix and RNA Index Adapters were added by incubating to end  cDNA repair. The cDNA fragments obtained from previous steps were amplified by  PCR, and products were purified by Ampure XP Bead, get the final library.