title: Transcriptional dysregulation associated with chromatin accessibility of iPSC and hepatocytes derived from a Cornelia de Lange syndrome patient (scRNA-Seq) [N]
design: Transcriptional dysregulation associated with chromatin accessibility of iPSC and hepatocytes derived from a Cornelia de Lange syndrome patient (scRNA-Seq) [N]
library_construction_protocol: iPSCs or differentiated cells were harvested with 1x Trypsine-EDTA Disaggregated cells (5·000-10·000 cells /sample) were applied to Single Cell RNA sequencing analysis using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10xGenomics, Pleasanton, CA).  Four samples were loaded on a single NextSeq 500/550 High Output Kit v2.5 flow cell, and analyzed on the Illumina NextSeq500 apparatus.