title: GSM6927925: AOL_1082; Mus musculus; RNA-Seq
library_construction_protocol: Organs were harvested at the indicated time points after cardial perfusion with PBS. For fluorescent activated cell sorting (FACS), MyD88pko mice and PodCre/wt x MyD88wt/wt controls were used. Due to low amount of input material, pre-amplification using the Ovation RNASeq System V2 was performed. Total RNA was used for first strand cDNA synthesis, using both poly(T) and random primers, followed by second strand synthesis and isothermal strand-displacement amplification. For library preparation, the Illumina Nextera XT DNA sample preparation protocol was used, with 1 ng cDNA input. After validation (Agilent 2200 TapeStation) and quantification (Invitrogen Qubit System) all six transcriptome libraries were pooled. The pool was quantified using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT system.