design: After crosslinking the samples, cell lysis was performed, and a portion of the sample was extracted to assess quality.Once the quality check was passed, the process proceeded to the Hi-C fragment preparation stage. The chromatin was digested using the restriction enzyme MboI (hereafter, any mention of restriction sites and digested fragments refers to this enzyme), and an aliquot was taken to evaluate the digestion efficiency. The Hi-C sample was then prepared through biotin labeling, blunt-end ligation, and DNA purification, followed by a quality assessment of the extracted DNA.Upon passing quality control, the sample proceeded to the standard library construction workflow. The Hi-C fragments underwent the removal of terminal biotin labels, ultrasonic fragmentation, end repair, A-tailing, and capture of biotin-labeled fragments. Sequencing adapters were then ligated to form adapter-ligated products. Finally, PCR conditions were optimized, and amplification was performed to obtain the final library products.