design: For RNA-seq, total RNA was extracted using the plant RNA purification kit (TaKaRa, 9769). The cDNA library was generated on the BGISEQ-500 platform of the Institute of genomics (www.genomics.org.cn), and the transcriptomes were sequenced using the paired-end methods. Bad or low quality data (Ns) (~5%) were removed from the raw data using the SOAPnuke (V1.5.2) software, and clean reads were mapped to the Arabidopsis genome (TAIR 10, https://www.ncbi.nlm.nih.gov/assembly/GCF_000001735.4 ) using HISAT (V2.1.0)