design: RNA was extracted from FFPE tumor tissue and stored at -80°C. Due to RNA degradation, a re-purification step was performed before library preparation. RNA libraries were prepared using the Illumina Stranded mRNA Prep and Exome Panel, followed by quality assessment with an Agilent TapeStation and quantitative PCR. Libraries were normalized, denatured, and sequenced on an Illumina NovaSeq 6000 instrument with a 150 x 150 cycle paired-end run.