design: The nuclei were isolated from frozen mouse brain tissue samples and constructed 3 single cell gene expression libraries (the latest GEM-X v4 version) using the 10x Genomics Chromium-X system. The libraries were sequenced with ~200 million PE150 reads per sample on Illumina NovaSeq X Plus. The sequencing reads were analyzed with the mouse reference genome GRCm39-2024-A using Cell Ranger v8.0.