design: Collection of industrial effluent contaminated soil samples, followed by DNA extraction using Dneasy power soil pro kit protocol, DNA quantification and QC using Gel electrophoresis and Qubit, Library preparation and validation using QIAseq FX DNA Library kit for illumina and purification using 1X AMPure XP beads kit. Validation by Qubit and it was done using Agilent D5000 Tapescreen system in Tapestation system (Catalog:G299AA, Agilent), followed by Sequencing was done by using Illumina NovaSeq 6000.