design: After DNA quality was assessed using Qubit and pulsed-field gel electrophoresis (PFGE), 15 g was used to construct a library using the SMRTbell Express Template Preparation Kit 2.0 with an average fragment length of 15 kb.  Using the PacBio Sequel II instrument, two SMRTcells were sequenced per library for an average of 43 Gb of HiFi reads per sample