design: TCR library preparation protocol consists of first a T4 ligation step to the 3 end of the TCR cDNA which allows the amplification of all possible rearrangements using a single set of primers per locus and the incorporation of a UMI attached to each cDNA TCR molecule that enables correction for PCR and sequencing errors. The final library was prepared for sequencing according to the Illumina NovaSeq protocol immediately prior to loading. Sample was loaded at 1.2 pM with 1520% of PhiX DNA at 1.8 pM. All samples were run in parallel, yielding 5 million reads per sample (2x150 bp paired end).