design: The whole genome was sequenced on the PacBio Sequel II System (https://www.pacb.com/products-and-services/pacbio-systems/sequel/) based on the single-molecule real-time (SMRT) sequencing technology. The template library was constructed using SMRTbell Template Prep Kit 1.0 (product code 100-259-100) and SMRTbell Damage Repair Kit (product code 100-465-900). Following the procedure described in the PacBio brochure >20 kb Template Preparation Using BluePippin Size-Selection System (15 -20 kb Cutoff) for Sequel Systems, the quality DNA was fragmented with g-TUBE (covaries, 520079) and concentrated with AMPure PB magnetic beads with the fragments eluted with the Pacific Biosciences Elution Buffer. The fragments were damage-repaired with ExoVII, end-repaired with End Repair Mix and ligated with the blunt adapter. After removing away the failed ligation products with ExoIII and ExoVII, the ligation products were purified twice with AMPure PB Beads, and subjected to size selection using BluePippin Size-Selection System. The yielded fragments were bead-purified, damage-repaired, and used as the ~20 kb SMRTbell templates. The templates were annealed with primers and bound to DNA polymerase using the PacBio DNA/Polymerase Kit and magnetic beads, and loaded onto PacBio Sequel Systems to read the sequence of templates.