design: Hi-C specifi ccapture: After the samples were tested and qualified, the library construction was carried out, and the main processes were as follows:1. cell cross-linking: fix the sample using formaldehyde, cross-link the intracellular protein with DNA and DNA with DNA to preserve their interaction relationship and maintain the 3D structure in the cell;2. endonuclease cleavage: DNA is enzymatically cleaved using restriction endonucleases to produce sticky ends on both sides of the crosslink. The restriction endonuclease generally used is DpnII;3. end repair: using the end repair mechanism, biotin-labeled bases are introduced to facilitate subsequent DNA purification and capture;4. cyclization: the DNA after end repair is cyclized between DNA fragments containing interactions to ensure that the location of the interoperable DNA is identified during subsequent sequencing and analysis;5. DNA purification and capture: Decrosslink the DNA, purify the DNA, fragment it into 300 bp -700 bp fragments, and use magnetic beads to capture the DNA fragments containing interactions for library construction.