design: HiC-sequencing libraries were generated using the Arima-HiC+ kit followed by the Swift Accel-NGS2S Plus library preparation kit with some modifications. 50-100 adult female flies were collected, flash frozen, and pulverised into a fine powder using a pellet pestle. Crosslinking, lysis, chromatin fragmentation, repair and biotinylation, ligation, shearing, and pull-down were all performed according to the Arima-HiC+ protocol. Libraries were generated using the Swift-Accel-NGS 2S Plus library preparation kit and PCR amplified for 8-10 cycles. The final libraries were then quality-checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific). The multiplexed libraries were sequenced as 150bp paired-end reads on the Illumina Novaseq 6000 SP.