design: DNA was extracted from 5 mcL of nectar samples using the DNeasy                 Blood and Tissue Kit (QIAGEN), following the manufacturer's instructions. The ITS2                 region (~100-400 bp) was amplified using the forward primers ITS3NGS1, ITS3NGS3, and                 ITS3NGS5, and the reverse primer ITS4NG (Tedersoo et al.,                 2014).