design: The DNA isolated from the supernatant and pellet fractions of the 2nd ampule of the 1916 mixed bacterial vaccine was amplified using Whole Genome Amplification (WGA) with the REPLI-g Advanced DNA Single Cell Kit (Qiagen, Germantown, MD). Illumina sequencing libraries were constructed from the amplified DNA using the ThruPLEX DNA-Seq Kit (Takara Bio USA, San Jose, CA) and sequenced on the Illumina MiSeq.