design: Three daughters of a female individual, all infected by Wolbachia, were dissected, and the DNA of their ovaries, nerve chains and hemolymph was extracted with the NucleoBond HMW DNA kit (Macherey-Nagel). The sequencing library was prepared using the SQK-LSK109 Ligation Sequencing Kit (Oxford Nanopore Technologies). The library was sequenced with an R9.4.1 flow cell on a MinION Mk1B. Reads were basecalled with Guppy 6.3.8 with the FAST model.