design: Single nuclei RNA-seq data was generated using the 10x Genomics Chromium platform. Vagal ganglia were dissected and pooled from 4-5 mice. Nuclei were isolated using Minute single nucleus isolatin kit. The samples were processed using the 10x Genomics Single Cell 3' RNA-Seq protocol, and the sequencing was performed on an Illumina NovaSeq 6000 with paired-end reads as described by the 10x user guide.