design: RNA was extracted using RNA PowerSoil Total RNA Isolation Kit (Qiagen, Valencia, CA, USA). TURBO DNA-free Kit (ThermoFisher) was used to remove genomic DNA and purified using RNeasy Plus MicroKit (Qiagen). rRNA was removed using Pan-Pro (RNA-Seq) riboPOOL 24 reaction Kit (siTOOLs Biotech). Then, RNA was prepared for sequencing using Superscript IV VILO Master Mix (ThermoFisher/Invitrogen). The library was prepared by Next Ultra II Directional RNA Library Prep Kit (NEB), and was sequenced on a NextSeq2000 (Illumina) platform in the Millard and Muriel Jacobs Genetics and Genomics Laboratory at the California Institute of Technology, generating 2150 bp paired-end reads with an average insert length of 300 bp.