design: Methods: 12 week-old mice were treated with gossypol at a dosage of 80 mg/kg for comparison with a control (DMSO) group. The gossypol solution was administered via oral gavage for two weeks at Nantong University, School of Life Sciences, Jiangsu, China. Following treatment, tissue samples from the liver, ovary, and testis were collected for transcriptome sequencing in three biological replicates. A total of 18 samples were quickly submerged in liquid nitrogen and stored at 80 C.