design: For lytic reactivation, subconfluent monolayers of iSLK-BAC16 were induced with 1 ug/mL Doxycycline, 1 mM Sodium Butyrate in 1x DMEM media supplemented with 2% Tet-approved FBS. 0 hour time point was when induction media was added and cells were first placed at 37 degrees C to incubate. RNA was collected at 0, 24 or 72 hours post induction. ERCC spike-in controls were added to total RNA.