design: Ten-day-old Arabidopsis WT (Col-0) seed seedlings were mock-treated or exposed to 0.5% DMSO for 3h. Approximately 20 million reads of 50 base pair (bp) paired-ended sequences were generated per sample. The raw reads were mapped onto the Arabidopsis gene model based on HISAT. Then, the number of reads mapped to each gene was then counted using HTSeq.