design: Illumina-compatible libraries constructed using NEBNext Ultra II DNA Prep Kit (E7645S) with 35050bp insert sizes. Fungal mycelia were enzymatically lysed with 0.1% chitinase (Sigma C6137) prior to CTAB-based DNA extraction. Size selection performed with ProNex beads (Promega NG2001) at 0.6/0.8 ratios. PCR amplification: 12 cycles using KAPA HiFi HotStart (Roche 07962355001). Sequenced on NovaSeq 6000 PE150. Qubit 4.0: 42.5 ng/L; Bioanalyzer DIN=8.2. Experimental details validated per NCBI Fungal Genome Submission Guidelines 2025.