design: The RNA was isolated using a trizol based method. The integrity and purity of RNA were determined by using an Agilent Bioanalyzer 2100 (Agilent, USA). A total amount of 200 ng of RNA per sample was used as input material for library preparation. mRNA was enriched using oligo(dT) beads. Subsequently, libraries were generated using the Novogene NGS  RNA Library Prep Set (PT042). Quality-controlled and quantified libraries, (using Agilent Bioanalyzer 2100, Qubit and qPCR method) were subjected to sequencing on the Illumina system (PE150, 20 M reads per sample).