design: The raw FASTQ read files were de-multiplexed into multiple files by barcode and a custom Perl script was used to extract usable reads from each BAM file. The reads were then aligned and mapped against the potato (Solanum tuberosum) reference  (http://spuddb.uga.edu/dm_v6_1_download.shtml) genome (Pham et al., 2020) using the Burrows-Wheeler Alignment (BWA), BWA MEM algorithm version 0.7.13r1126 (Li and Durbin, 2010, Li, 2013). Reads were converted from BAM to fastq.