design: Add an appropriate amount of Trizol at a ratio of 1ml Tirzol per 2  106 cells to fully digest the cells and extract total RNA.Transfer the cell suspension to a 1.5ml RNase free centrifuge tube, use a 1ml pipette tip to repeatedly blow and mix until Trizol is clear and transparent. Mark the sample and store it in a -80  freezer.Perform quality checks on the purity, concentration, and integrity of the extracted total RNA. After strict quality control, use the Illumina HiSeq 4001 platform to build a library and obtain raw sequencing data files in FASTQ format