design: Tumor DNA was derived from the BM where the percentage of blast cells was higher than 80%. Germline DNA was obtained from the tail when the mice were 4-weeks old (pre-leukemic). Genomic DNA libraries were prepared from sheared DNA with the HyperPrep Library Preparation Kit (Roche PN 07962363001). Paired-end 150 cycle sequencing was performed on a NovaSeq 6000 (Illumina). Illumina paired-end reads were preprocessed and were mapped to the mouse reference genome (mm10) with BWA.