design: Genomic DNAwas extracted using a Phenol-Chloroform-based extraction protocol.Before librray preparation, purified genomic DNA and spike in DNA were combined and sonicated using Covaris S2 to create 200300 bp fragments.  The NEBNext Enzymatic Methyl-seq (EM-seq, New England BioLabs) kit was utilized to construct Methyl-seq libraries. Before generating the libraries,