design: The shotgun type DNA library was prepared with the NEBNext UltraTM II FS DNA Library Prep Kit for Illumina (New England BioLabs inc.) following the manufacturer's protocol. Ten ng of DNA was used as starting material for library preparation. The fragmentation was optimized to obtain inserts of 100-250bp size. A size selection step with AMPure XP beads (Beckman Coulter) was added after the adapter ligation. A Unique Dual Index Primer Pair (UDI) was used during the PCR enrichment step (New England BioLabs inc.). Libraries were quantified using the QubitTM dsDNA HS Assay Kit (InvitrogenTM). Average size fragment was determined using a Bioanalyzer (Agilent) instrument. The library was sequenced with a depth of 50M reads on NovaSeq 6000 S1 (v1.5): paired end 100pb.