design: DNA extracted from homogenized bulk ichthyoplankton samples using salt extraction protocol, 1st PCR amplification using MiFish 12S or modified PS1 Teleost Primers with heterogeneity spacers and Nextera adaptor sequences, 2nd PCR using combinatorial Nextera i5/i7 indices, purified using Ampure XP magnetic beads and quantification via QuBit broad range kit, Illumina MiSeq using the V2 (150bp x 2)