design: Genomic DNA (gDNA) was extracted using the QIAamp DNA Micro Kit (#56304). The PCR amplification was performed with AmpliTaq Gold 360 Master Mix (#4398876) under specific cycling conditions: an initial denaturation at 95C for 10 minutes, followed by 40 cycles of denaturation at 95C for 30 seconds, annealing at 60C for 30 seconds, and extension at 72C for 30 seconds, concluding with a final extension at 72C for 7 minutes. The following sequences were used to make amplicon: F-CAGAGAAACCGGCATGATGG and R- TGTCACATGGTTGCACTTAGG. Following PCR, the amplicons underwent hybridization witha detailed temperature protocol: initial denaturation at 95C for 5 minutes, a gradient annealing from 95C down to 85C at a rate of -2C per second, and then from 85C down to 25C at -1C per second. After hybridization, T7 Endonuclease I was added to each sample and incubated for 30 minutes at 37C. Finally, the samples were analyzed on a 2% agarose gel. PCR products were sequenced by PCR amplicon sequencing in MGH CCIB DNA Core.