design: gDNA was isolated using the QIAamp DNA Mini Kit. The DNA quality was checked on Fragment analyzer and fragmented to the desired length using Biouptor pico. The library preparation for sequencing was done on Biomek FX with the Kapa hyper prep kit for DNA. Truncated universal stub adapters were used for ligation, and indexed primers were used during PCR amplification to complete the adapters and enrich the libraries for adapter-ligated fragments. Samples were checked for quality on an AATi Fragment Analyzer. Quantitative PCR was used to calculate sequence-able morality with the Kapa Biosystems Illumina Quant qPCR Kits. Libraries were pooled evenly by molarity and sequenced on an Illumina NovaSeq6000 150PE S4.