design: Using 5-10g of high-quality genomic DNA fragmented into ~10Kb segments (or 20Kb for large genomes) via Covaris g-TUBE, a SMRTbell library was constructed with the DNA Template Prep Kit, then loaded onto a PacBio Sequel sequencing chip via passive diffusion with sequencing primers and polymerase for single-molecule real-time (SMRT) sequencing.