design: To generate chromatin conformation data, we first isolated nuclei following the protocol from Workman et al. (2019). Approximately 1g of sterile leaf tissue was ground in liquid nitrogen for nuclei isolation; we ensured that intact nuclei were obtained by staining with DAPI and examining under a fluorescent scope. The pelleted nuclei were washed with PBS, snap frozen in liquid nitrogen, and then used as the input into the Omni-C Mammalian Cell protocol following the manufacturers protocol (Dovetail Genomics, CA, USA) using a 1:10 dilution of the nuclease enzyme mix and 25-30 min digestion.