design: The genomic DNA was extracted using the Genomic-tip 20/G system (Qiagen). A genomic DNA library for sequencing was prepared and multiplexed using the rapid barcoding kit (product no. SQK-RBK004, Oxford Nanopore Technologies). The library was sequenced with FLO-MIN111 flow cell (Oxford Nanopore Technologies) on a GridION X5 device (GridION software release 21.10.5, Oxford Nanopore Technologies), base-called, demultiplexed, and adapter-trimmed by guppy version 6.3.8 (Super-Accurate Mode).