design: Tempus xT panel DNA library construction and sequencing of FFPE PDAC and matched normal blood. DNA sequencing of 596 genes was performed as previously described [Beaubier N, et al. Oncotarget. 2019; 10,2384-96]. Briefly, 100 nanograms (ng) of DNA for each tumor sample was mechanically sheared to an average size of 200 base pairs (bp) using a Covaris Ultrasonicator. DNA libraries were prepared using the KAPA Hyper Prep Kit, hybridized to the xT probe set, and amplified with the KAPA HiFi HotStart ReadyMix. The amplified target-captured DNA tumor library was sequenced using 2x126bp PE reads to an average unique on-target depth of 500x (tumor) and 150x (normal) on an Illumina HiSeq 4000. Samples were further assessed for uniformity with each sample required to have 95% of all targeted bp sequenced to a minimum depth of 300x.