design: The Omni-C library was prepared using Dovetail TM Omni-C TM Kit (Dovetail Genomics, ScottsValley, CA) according to the manufacturers protocol with slight modifications. Briefly,chromatin was fixed in place in the nucleus. Fixed chromatin was digested with DNase I thenextracted. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followedby proximity ligation of adapter containing ends. After proximity ligation, crosslinks werereversed and the DNA was purified from proteins. Purified DNA was treated to remove biotinthat was not internal to ligated fragments. An NGS library was generated using an NEB Ultra IIDNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor. Biotin-containing fragments were then captured using streptavidin beads. The post capture productwas split into two replicates prior to PCR enrichment to preserve library complexity with eachreplicate receiving unique dual indices. Sequencing was performed at the Center for AdvancedTechnologies at the University of California, San Francisco (UCSF CAT) on an Illumina NovaSeq Xplatform (Illumina, CA) to generate approximately 100 million 2 x 150 bp read pairs per GBgenome size.UCSF Acknowledgment (per their request)Deep Sequencing of the Omni-C libraries was performed at the UCSF CAT, supported by UCSFPBBR, RRP IMIA, and NIH 1S10OD028511-01 grants.