design: Adult sponges were collected in the summer from the underside of a ferry dock in Lake Constance near Kressbronn, Germany on 14 July 2023 and kept in cool water for one day until processing in the lab, where individual sponge were dissected to remove other animal contaminants, flash frozen, and stored at -80C until used for experiments. RNA was extracted using the Qiagen DNA/RNA Allprep Micro Kit (Qiagen #80284), and the resulting extracts were quantified using a Nanodrop 1000. RNA integrity was assessed on the 5300 Fragment Analyzer with RNA Quality number of 9.6. Isoseq cDNA was made using the Kinnex iso-seq cDNA kit and quality was checked using the Agilent High Sensitivity D5000 ScreenTape. Sequencing was performed at the Yale Keck Microarray Shared Resource Facility on a PacBio Revio.