design: NEBnext Ultra II FS DNA library preparation kit following manufacturers specifications, with 10-100ng of input DNA and enzymatic fragmentation for 10 minutes to achieve average insert sizes of 300-400 bp. Final amplification consisted of 4-6 cycles as recommended by the manufacturer. Twenty-eight individually dual-indexed genomic libraries were pooled and sequenced on a single lane of the NovaSeq S4 300 cycle to achieve 60X coverage using 2 x 150 bp paired end reads.