design: DNA was extracted using the AllPrep DNA/RNA Mini kit (Qiagen #80204). DNA was quantified using the Qubit Flex Fluorometer (Invitrogen), DNA purity was determined using a NanoDrop Eight (Thermo Scientific) and DNA integrity was measured using a 4200 Tapestation (Agilent). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645) and enriched using SureSelectXT Human All Exon V7 (Agilent #5191-4004) probes. Library sizes and quantification were determined by 4200 Tapestation (Agilent), and libraries were subsequently pooled equimolar. The final pool was loaded onto one lane of an S4 v1.5 flow cell (300 cycles) and paired-end sequencing was performed with reads lengths of 150 base pairs (Illumina; 20028312).