design: Tempus RNA library construction and sequencing of FFPE PDAC. RNA sequencing was performed as previously described [Beaubier N, et al. Oncotarget. 2019; 10:2384-96]. One hundred ng of RNA for each tumor sample was heat fragmented in the presence of magnesium to an average size of 200 bp. Library preps were hybridized to the xGEN Exome Research Panel v1.0 (Integrated DNA Technologies) and target recovery was performed using Streptavidin-coated beads, followed by amplification with the KAPA HiFi Library Amplification Kit. The amplified target-captured RNA tumor library was sequenced using 2x75bp PE reads to an average of 50M reads on an Illumina HiSeq 4000.