design: DNA extraction was performed using the PowerSoil DNA Isolation Kit (Mobio) according to the manufacturers instructions, using 200 mg of processed roots and 500 mg of soil. Additionally, two negative controls were prepared by performing DNA extraction with only the kit reagents, without any sample addition, to evaluate possible contamination. Purified DNA was quantified using a Qubit 4 Fluorometer (ThermoFisher).The bacterial community composition was determined by amplification and sequencing of the V5-V7 region of the 16S rRNA genes, using primers 799F (5-AACMGGATTAGATACCCKG-3) and 1193R (5-ACGTCATCCCCACCTTCC-3)