design: Library preparation and sequencing were performed using the NEB Ultra II FS DNA PCR-free Library Prep Kit following the standard protocol on a Hamilton Liquid Handling Robot except for modifications: SPRI cleanup following ligation was performed with a single sided cleanup step, following library preparation 10 cycles of PCR were performed to amplify reads. Libraries were pooled at 120pM in 1,000uL and sequencing was performed on an UltimaGenomics sequencer with the Baseline 1.5 workflow, performing 116 cycles for 300bp length read sequencing.