design: RNA was isolated from Col-0WT  plants that had been grown 10 days in 0 ppm PFOS before PFOS treatment plants were transferred to sterile water for 1 days. Water was replaced by 20 ppm PFOS for 3 or 6 hr. Ten seedlings were pooled for each biological replicate, and three biological replicates per treatment were sequenced. Seedlings were ground in liquid nitrogen and RNA was isolated using the Spectrum Total RNA Extraction Kit (Sigma Aldrich). RNA libraries were made using poly-A selection. 150 bp paired end reads were sequenced.