design: RNA quality control and quantification was performed on the TapeStation 4100, and libraries were prepared with 100 ng input using the QuantSeq 3 mRNA-seq Library Prep Kit. Prepared RNA libraries were sequenced on the NovaSeq 6000 (>10M reads per sample). Reads were aligned to the mouse genome (mm10) with STAR for alignment followed by ht-seq count for tabulation for each gene region.