design: Environmental DNA extracted following Miya et al. (2016) cartidge filter protocol using QIAamp mini DNA kit (QIAGEN). Sequenced with a mutliplex of Miya et al. (2015) MiFish-U-F, MiFish-U-R and a custom primer (GIQHerp-F) to target fish and herptile taxa. Library prep consisted of 3-step PCR: (1) enrichment of target, (2) annealing of Illumina dapters, (3) incorperation of unique barcode sequences. Run on Illumina MiSeq system v2 300-cycle chemistry, library loaded at 8pM with 10% PhiX control spike in.