design: Genomic DNA was extracted following procedures described in the FastDNA SPIN for Soil DNA Extraction kit (MP Biomedicals). The extracted DNA quality was assessed based on the parameters of purity (260/280 nm ratio) and quantity (ng L?) for each sample. Those were measured between 1.8 and 2.0 using a Nanodrop 2000 spectrophotometer (Nanodrop Inc., Wilmington, DE). The 0.8% agarose gel electrophoresis was also carried out to confirm integrity. NGS Genomic Solutions (Piracicaba, SP/ Brazil) processed the sequencing following Illumina Inc.s protocol. HiSeq Illumina paired-end sequencing of the V4 region (400-450 bp) of 16S rRNA with primers 515F e 806R (CCTAYGGGRBGCASCAG, GGACTACNNGGGTATCTAAT). The sequencing was analyzed in R language and the taxonomic classification was referenced using the SILVA database (DADA2 software).