design: Genomic DNA samples were quantified using the Qubit Fluorometer 3.0 and approximately 25ng of genomic DNA was used for sample assessment. Automated dual indexed libraries were constructed with ~300-500ng of genomic DNA utilizing the KAPA Hyper Prep Library Kit (Roche Diagnostics, Cat # 7962363001) on the SciCloneG3 NGS NGS instrument (Perkin Elmer) targeting 250bp inserts.  Libraries were pooled (500ng each) pre-capture generating  1-2g library pools.  Each library pool was hybridized overnight with the xGen Exome Hyb Panel v1 (IDT, Cat # 1056115) and 1 ul of the diluted TERT promoter probe. The molarity of each library capture pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit (Roche Diagnostics, cat # 7959478001/ 7960085001) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument.  Normalized library pools were sequenced on a NovaSeq6000 S4 Flow Cell using the XP workflow and a PE 151 cycle sequencing recipe according to manufacturer protocol targeting 24Gb per library.