design: Barley (Hordeum vulgare cv. RGT Planet) grown in glasshouse for three weeks. Sample comprised of 24 (2 mm) biopsy punches taken from leaf tissue; 24 punches were made up from 3 punches taken from two regions 10-15 cm from the tip of the second leaf from 4 leaves, each from a different plant. Total RNA was extracted using Norgen Plant/Fungi Total RNA Purification Kit. Library was prepared using the Agilent SureSelect XT HS2 mRNA Library Preparation Kit and sequenced on an Illumina NovaSeq 6000 (paired end, 150 bp).