design: RNA-sequencing libraries were generated using the NEBNext Poly(A) mRNA Magnetic Isolation Module followed by the NEBNext Ultra II Directional RNA Library Kit for Illumina. Briefly, adult females were dissected in ice-cold PBS and 40-50 ovaries were flash-frozen and stored at -80C until further processing. Total RNA was collected from the Lexogen TraPR Small RNA isolation columns using TRIzol LS (after small RNA isolation), and subsequently purified with the addition of chloroform and isopropanol. After purification, 2.5g of RNA was measured by Qubit RNA HS Assay kit (Thermo Scientific) and used for poly(A) isolation and library preparation. Libraries were amplified for 8 cycles, and quality checked and quantified for multiplexing using the Bioanalyzer High-Sensitivity DNA kit (Agilent) and Qubit dsDNA HS Assay kit (Thermo Scientific). The multiplexed libraries were sequenced as 150bp paired-end reads on the Illumina Novaseq 6000 SP.