design: Next-generation sequencing libraries were prepared using the 10x Genomics Chromium Single Cell 3 Reagent Kit v3 according to the manufacturer's instructions. Libraries were uniquely indexed using the Chromium Dual Index Kit, pooled, and sequenced on an Illumina NovaSeq 6000 sequencer using a paired-end, dual-indexing run strategy. Sequencing for each library aimed for 20,000 mean reads per cell. The data were then processed using the Cell Ranger pipeline (10x Genomics, version 6.1.2) for demultiplexing, alignment of sequencing reads to the mm10 mouse transcriptome, and generation of feature-barcode matrices