design: purified RNA was treated with DNase I to remove genomic DNA. cDNA libraries were prepared using the Illumina TruSeq Standard mRNA Library Prep Kit (Illumina Inc., San Diego, CA, USA) and sequenced with Illumina NovaSeq 6000. The raw reads generated underwent an initial quality check using FastQC, and then adapter and low-quality sequences were removed using Trimmomatic. After preprocessing, the trimmed reads were aligned to the Mus musculus (mm10) reference genome using HISAT2 v2.1.0.