design: Libraries for NGS were prepared using a KAPA HyperPlus Kit (Roche, USA) according to the manufacturers protocol. DNA fragmentation time was 15 minutes to achieve the average fragment length of 350 bp. Target enrichment was carried out using KAPA HyperCap hybridization probes (Roche, USA). Mass parallel sequencing was performed on the MiSeq platform (Illumina, USA)