design: Total RNA was extracted using the PureLink RNA Mini Kit (Ambion, Life Technologies, California USA) following the manufacturers instructions and sent to Genoma Mayor Sequencing Services (Santiago, Chile), where it was purified to retain only mRNA. RNA quality and quantity were determined using an Agilent 2100 Bioanalyzer (Agilent Technologies, California USA). Samples with an RNA integrity number >7 were subjected to 2100 bp sequencing (Illumina HiSeq 4000).