design: Soil DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA). DNA was fragmented to150 bp using the Covaris M220 (Gene Company Limited, China), and paired-end libraries were constructed using the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific, USA). Sequencing was performed on the DNBSeq G898 platform (BGI Genomics, Shenzhen, China).