design: HDLEC (LEC) cells were infected with KSHV strain BAC16 at an MOI of 1 (70% cells infected). Virus was adsorbed in a low volume of media for 8 hr at 37 degrees C, after which viral inoculum was removed and replaced with fresh media. 0 hour time point was when virus was added and cells were first placed at 37 degrees C to incubate. Total RNA was isolated at 24 hours post infection using the Direct-zol RNA MiniPrep Kit. ERCC spike-in controls were added to total RNA. RNA was ribominus selected and directional cDNA libraries were generated using either Stranded Total RNA Prep with Ribo-Zero Plus (Illumina # 20040525) or TruSeq Stranded Total RNA Ribo-Zero Gold (Illumina #RS-122-2303).