design: DNA extraction was performed using the Qiagen DNeasy PowerSoil Pro Kit. Bacterial glycerol stocks were vortexed, pelleted, and resuspended before DNA was extracted and eluted in 50 L. DNA quality and quantity were assessed using Qubit and NanoDrop. Bacterial identity was confirmed via Sanger sequencing of 16S rRNA gene fragments. Libraries were prepared for whole genome sequencing using the Illumina DNA Prep (M) Tagmentation Kit with indexed adapters. Library quality was assessed using the TapeStation, pooled at 4 nM, and denatured to 8 pM before sequencing on an Illumina MiSeq system using V2 chemistry (2x250 bp). Sample was sequenced in triplicate to maximize depth.