design: T cells were sorted from the cloudy effluent sampled from peritoneal dialysis patients presenting with acute peritonitis. In brief, density gradient centrifugation with Lymphoprep (Axis-Shield) was used to isolate peripheral blood mononuclear cells from cloudy effluent peritoneal dialysis fluid. Cells were stained with the following reagents: anti-CD14V500 (clone M5E2), anti-CD19V500 (clone HIB19), anti-CCR7PE-Cy7 (clone 3D12) and anti-V2-PE (clone B6) from BD Biosciences; anti-CD4PE-Cy5.5 (clone S3.5), and LIVE/DEAD Fixable Aqua from Thermo Fisher Scientific; anti-CD3APC/Fire 750 (clone SK7), anti-CD8BV711 (clone RPA-T8), anti-CD45RAPE-Dazzle 594 (clone HI100), anti-CD27BV421 (clone M-T271), anti-TCR-PE-Cy5 (clone B1) and anti-V7.2-BV605 (clone 3C10) from BioLegend; anti-CD161-APC (clone 191B8) from Miltenyi; and anti-CXCR3-FITC (clone 49801) from R&D Systems. ViableT cells expressing V2 were identified in the CD3+ lineage and sorted into RNAlater (Thermo Fisher) using a custom-built FACSAria II (BD Biosciences) equipped with FACSDiva software version 8.0 (BD Biosciences). RNA extraction as well as amplification and TCR sequencing was done by iRepertoire (MiSeq v2 500 cycle flow cell).