design: Our HiChew design process simplifies down to four key steps before PCR. First, we crosslink the DNA strands together by formaldehyde. Next, we treat the DNA on the GATC motif using DpnII. Then, we connect nearby digested DNA ends, leaving behind a GATC ligation scar this is based on the principle of proximal ligation. After cell breakdown, we can choose either a ligation-based or Tn593-based DNA library construction method, depending on the amount of DNA and your specific requirements.