design: For MinIONTM sequencer, sheared DNA fragments were straightly end-repaired, 3 adenylated, and then ligated to adaptors pre-loaded with motor proteins. The product was purified using Agencourt AMPure XP Beads (Beckman, A63881). Finally, fragments larger than 1 Kb were screened for single-molecule nanopore DNA sequencing using Multiplex Ligation Sequencing Kit (SQK-MLK111.96-XL) on MinION Flow Cell (ONT, R9.4.1). Basecalling was performed using Guppy version 6.5.6 with default model (ONT). A total 137,688 reads were obtained, followed by trimming using Porechop v0.2.4, and filtering using NanoFilt v2.8.0.