design: The method for analyzing exogenous insertion fragments is to use software pbmm2 to align Reads to the insertion fragment in order to obtain Redas that can determine the insertion site. Then, these Reads are aligned to the reference genome and insertion fragment using Blast, and filtered under the following conditions: 1 This reads can be compared to both inserted fragments and reference genes. 2. The length of the reads aligned to the insertion sequence is greater than 100bp, and the length aligned to the reference genome is greater than 1000bp.Filter according to the above conditions and organize the comparison of several modes in the report.