design: DNA was extracted using the MagMAX Microbiome Ultra Nucleic Acid Isolation kit with bead beating.  Sequencing libraries were prepared using the Oxford Nanopore SQK-LSK114 native barcoding kit with Long Fragment Buffer.  Libraries were sequenced on the GridION platform using a FLOW-MIN114 Spot-ON flow cell (R10) with a translocation speed of 400 bps.  Base calling was performed on the GridION using the super-accurate base calling model.