design: We used total RNA extracted from 2,000,000 T cells for cDNA synthesis with a common 5-RACE adapter using the SMART Library Construction Kit (Clontech Laboratories, Mountain View, CA, USA). TCR and TCR cDNAs were amplified by PCR using a forward primer for the SMART adapter and a reverse primer corresponding to the constant region of the TCR and TCR genes. Illumina index sequences with barcodes were added using the Nextera XT Index kit (Illumina, San Diego, CA, USA), and the prepared libraries were sequenced by 300-bp paired-end reads on the Illumina MiSeq System using MiSeq Reagent v3 600-cycles kit (Illumina).