design: DNA was digested using 3 enzyme combinations (PstI/MseI, KasI/AciI and KasI/HpyCH4IV) following a protocol with steps modified from Peterson et al. (2012). DNA samples were individually ligated to specific P1-n barcoded adapters, size selected using Nucleomag NGS clean-up and size select kit (Macherey-Nagel). Adapter-ligated fragments were then PCR-amplified with uniquely indexed PCR-P2 primer sequences to allow for post-sequencing demultiplexing. PCR products were then quantified using Qubit dsDNA HS assay kit, and then size-selected with Pippin-Prep (1.5% agarose dye free gel cassette, Sage Science, Beverly, Massachusetts, USA).