design: All samples consisted of previously extracted genome provided by the respective manufacturer (American Tissue Culture Collection (ATCC) Virginia, USA and Zymo Research California, USA). Sequencing libraries were prepared using Oxford Nanopore Rapid Sequencing gDNA kit (SQK-RAD004) according to the manufacturers specifications. Sequencing was performed on a MinION MK1C device using a R9.4.1 version flow cell. Sequences were collected for 1 hour.