design: Libraries for scRNA-seq were prepared using the 10x Genomics Chromium Single Cell 3' Library and Gel bead Kit V2 (CG00052_RevF) according to the manufacturer's protocol for each human prostatectomy tissue punch. The cDNA libraries were sequenced (150 bp paired-end) on the Illumina HiSeqX platform. Each sequenced library was demultiplexed to FASTQ files using Cell Ranger (10x Genomics).