design: The mRNA profiling of ac4C in lung adenocarcinoma (LUAD) is unknown. We made the acetylated RNA immunoprecipitation sequencing to identify ac4C peaks on mRNA of human LUAD tissues and adjacent non-tumor tissues, and differences of acetylation and mRNA between the two groups were analyzed. In addition, bioinformatics analysis was conducted to explore the function of ac4C-specific acetylated transcripts. Our study revealed that the degree of ac4C in mRNA in LUAD was significantly higher than in adjacent tissues and was concentrated mainly in the coding sequences with a implications in a wide range of cellular functions.