design: Sample libraries were prepared using QuantSeq 3 mRNA-Seq V2 Library Prep Kits (Lexogen, GmbH, Austria) with Unique Dual Index (UDI) and QuantSeq Unique Molecular Identifiers (UMI). Completed libraries were QCd and quantified using a combination of Qubit dsDNA HS and Agilent 4200 TapeStation HS DNA1000 assays. Sequencing was performed in a 1x100bp single read format using Illumina NovaSeq SP flow cells (Illumina, Inc., San Diego, CA, USA).