title: Variable effects of ectomycorrhizal fungi on litter decomposition in temperate pine forests
design: To identify the ectomycorrhizal and saprotrophic fungi present across sites and treatments, total genomic DNA was extracted using Qiagen DNEasy PowerSoil kits. The ITS2 rDNA subunit was PCR amplified using a barcoded fungal-specific ITS5.8SFun-ITS4Fun primer set under cycling conditions detailed in Taylor et al. (2016). Amplified products were magnetically cleaned using the Agencourt AMPure XP kit and quantified using a Qubit dsDNA HS Fluorometer. Each of the samples were pooled into a single library and sequenced at the University of Minnesota Genomics Center using the 2 x 300 bp paired-end MiSeq Illumina platform.