title: RNAseq of WT HEC-1-A endometrial cancer cells + Beta-cateninD32N
design: NT5E WT and NT5E KO triplicate cultures of HEC-1-A cells were transfected at 80% confluency with either empty vector, D32N b-catenin, G34R b-catenin, or S37F b-catenin. RNA was extracted from cells 48 hours post-transfection using the RNeasy Plus Kit and QIAshredder columns according to manufacturers instructions. RNA sample QC and mRNA library preparation was performed by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers and the Illumina NovaSeqX PE150 platform was used for sequencing.