title: soil_R.solanacearum_UW551_and_bacteriophage_PYO4.PYO59_replicate5
design: The 16S sequencing and initial bioinformatic analysis were performed by Novogene using a PE250 strategy on an Illumina Novaseq 6000 using the primers Forward GTGCCAGCMGCCGCGGTAA and Reverse GGACTACHVGGGTWTCTAAT targeting the V4 of the 16S ribosomal RNA. PCR was performed, and the size assesses by 2 % agarose gel electrophoresis. Then the same amount of PCR product was pooled for each sample, end-repaired, A-tailed and ligated to Illumina adapters. Library was quantified using Qubit and RT-qPCR whilst Bioanalyser was used to assess the size of the library.