title: RNA-Seq of apple: Auxin applied group day 1
design: Transcriptomic Analysis by RNA Sequencing RNA isolation from apple seeds was performed using the RNeasy Plant Mini Kit (Qiagen, USA), and DNA cleanup was performed according to the instructions of the RNase-Free DNase Kit (Qiagen, USA). The quantity and quality of total RNA were determined using Nanodrop (USA) and visualized by 2 % agarose gel electrophoresis. mRNA libraries were prepared using The Ribo-Zero rRNA Removal Kit (Plant) (EpiCentre, Biotechnologies, America) and The NEBNext UltraTM RNA Library Prep Kit for Illumina (New England Biolabs, Beijing, China) compatible with the Illumina HiSeq4000 system.