title: Pore-C data
design: 10 L of nucleated blood collected from a Bengalese Finch was used in the Pore-C restriction enzyme protocol (v4) from ONTs website. The protocol was modified by using only  of the recommended NIaIII enzyme due to limited inventory. Custom bead selection was performed at the end of the protocol, and Elution 1 from the sample was used for library preparation. Library preparation was carried out using the Ligation Sequencing Kit V14 (SQK-LSK114) following the ONT protocol (GDX_9163_v114_revI_29Jun2022). The AMPure XP steps were modified to use a 1X volume to ensure retention of smaller DNA fragments. Sequencing was performed on an R10.4.1 Flow Cell (FLO-PRO114M) on the PromethION 48 sequencer (PRO-SEQ048). The flow cell was washed using the ONT wash kit (EXP-WSH004) and reloaded with fresh libraries every 24 hours for a total runtime of 96 hours.