title: 16S rRNA gene amplicon sequencing of total culturable bacterial community (plate-wash strategy, PW) under microaerophilic (M) culturing conditions from Leptogorgia sarmentosa
design: Amplicon library preparation followed Illuminas 16S Metagenomic Sequencing Library Preparation protocol. The V4 region of the 16S rRNA gene was amplified with primers 515F/806R (Earth Microbiome Project protocol) and sequenced on an Illumina MiSeq platform.