title: scRNAseq of hek293t wt
design: Single-cell RNA-seq was performed using the Chromium Single Cell 3' Solution (10x Genomics) following the manufacturers protocol. Briefly, single-cell suspensions of HEK293T cells and HEK293T-CTCF+/- cells were loaded onto a Chromium Controller to generate nanoliter-scale Gel Bead-in-Emulsions (GEMs), enabling the encapsulation of individual cells with barcoded beads. After cell lysis, RNA molecules were reverse-transcribed to cDNA, incorporating unique molecular identifiers (UMIs) and cell-specific barcodes. The resulting cDNA was amplified and used for library preparation following the Chromium Single Cell 3' Library Construction Kit protocol. Libraries were  circularized, prepared DNBs and sequenced on an MGISEQ-2000 platform to generate paired-end 100 bp reads.