title: Amplicon metabarcoding (MiSeq) of prokaryotic metagenome (16SV4): stream substrates
design: 16S v4 targeted using 515F and 806R primers based on Parada et al. 2016 and Apprill et al. 2015. Parada, A. E., Needham, D. M., & Fuhrman, J. A. (2016). Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environmental microbiology, 18(5), 1403-1414. Apprill, A., McNally, S., Parsons, R., & Weber, L. (2015). Minor revision to V4 region SSU rRNA 806R gene primer greatly increases detection of SAR11 bacterioplankton. Aquatic microbial ecology, 75(2), 129-137.