title: Transcriptome sequence of Crassostrea virginica cg_7
design: For mRNA enrichment and cDNA synthesis, 7 ul of total RNA per sample (containing 30 g RNA on average) was processed using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England BioLabs). Library quantification was performed with the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems), and fragment sizes were checked with a LabChip GX (PerkinElmer) instrument. Libraries were normalized, pooled, denatured in 0.05N NaOH, and neutralized with HTI buffer.