title: Shot-gun sequencing for DNA extracted from marine sediments. DNA extracted with the QIAamp PowerFecal DNA kit (#12830-50 Qiagen) and purified further with the Evrogen DNA clean-up kit.
design: Libraries for shotgun sequencing were prepared from 300500 ng of staring DNA using MGIEasy Universal DNA Library Prep Set (MGI Tech), following the manufacturer's instructions. DNA was sonicated using a Covaris S-220 followed by selection of 200-250 bp-long fragments on magnetic beads (MagBio). The concentration of the prepared libraries was measured using Qubit Flex (Life Technologies) with the dsDNA HS Assay Kit. The quality of the prepared libraries was assessed using Bioanalyzer 2100 with the High Sensitivity DNA kit (Agilent). DNA libraries were further circularized and sequenced by a paired end sequencing using DNBSEQ-G400 with the High-throughput Sequencing Set PE100 following the manufacturer's instructions (MGI Tech) with an average coverage of 100x. FastQ files were generated using the zebracallV2 software (MGI Tech).