title: PacBio Transcriptome Sequencing of Patagonian toothfish
design: Using the SMARTer PCR cDNA Synthesis Kit(Clontech 634925), RNA is synthesized to cDNA. 1 g of total RNA used in each cDNA synthesis reaction. The SMRTbell library was sequenced using SMRT 2 cells and extracted High Quality Isoseq sequences.