title: cDNA
design: Total RNA was extracted from each sample using the TRIzol reagent (Invitrogen, CA, USA) in accordance with the manufacturers instructions. Using the Agilent 2100 Bioanalyzer and NanoDrop 2000 spectrophotometers from Thermo Scientific and Thermo Scientific, respectively, the purity, quantity, and integrity of RNA were assessed. After that, the libraries were built with the VAHTS Universal V6 RNA-seq Library Prep Kit in accordance with the manual provided by the manufacturer. Following the libraries 150 bp paired-end read generation on an Ilumina Novaseq 6000 platform, the poor-quality raw reads were eliminated to produce the clean reads.