title: DKO enterololin-resisant mutant: LolC V2
design: E. coli BW25113 bamBtolC was grown in 3 ml LB medium overnight at 37 C with shaking. ~108 CFU in 100 l liquid LB was deposited onto solid LB plates supplemented with enterololin at varying concentrations ranging from 0.0625 g ml1 to 2 g ml1. Plates were incubated at 37 C and monitored every 24 h for the emergence of enterololin mutants. On the emergence of colonies, these were purified by re-streaking onto solid LB and solid LB supplemented with enterololin at the same concentration from which the colonies were originally collected. Seven independent enterololin-resistant strains were subsequently selected for whole-genome sequencing.