title: transcriptomic sequencing
design: RNA was extracted from the bacterial samples using Invitrogen TRIzol RNA Kit, and the total RNA was examined for quality, and then the rRNA was removed from the total RNA using Zymo-Seq RiboFree Total RNA Library Kit. rRNA was fragmented by ion interruption and used for the construction of libraries. The quality of the constructed libraries was checked using an Agilent 2100 Bioanalyzer and an Agilent High Sensitivity DNA Kit. The libraries were subjected to Paired-end (PE) sequencing using Next-Generation Sequencing (NGS) technology based on the Illumina sequencing platform.