title: D0 reference
design: The genomic regions flanking the targeted K/S/T/Y codons were PCR amplified and sent for NGS. The nucleotides around the target codons were extracted and analyzed, then translated into protein sequences. Following sequencing, the nucleotides adjacent to the target codons were extracted for analysis and translated into corresponding protein sequences. To assess mutation frequency, we calculated the percentage of mutated reads relative to the total matched reads.