title: RNA seq of Dunaliella salina cultured under blue light for 15 days
design: After the sample testing is qualified, the library construction is carried out, and the main process is as follows: (1) Enrichment of eukaryotic mRNA using magnetic beads with Oligo (dT); (2) Add Fragmentation Buffer to randomly interrupt mRNA; (3) Using mRNA as a template, synthesize the first and second cDNA strands, and purify the cDNA; (4) The purified double stranded cDNA was subjected to terminal repair, followed by the addition of an A tail and ligation of sequencing adapters, and then fragment size selection was performed using AMPure XP beads; (6) Finally, the cDNA library was obtained by PCR enrichment.