title: Single nuclei RNA-seq of Anopheles gambiae : adult female fat body - Injected with E.coli and M. luteus
design: This study used single-nucleus RNA sequencing to investigate cell-specific responses in the female abdominal fat body of Anopheles gambiae following bacterial challenge with E. coli and M. luteus. Fat body nuclei were isolated from challenged and PBS-injected control mosquitoes 4 hours post-injection and sequenced using the 10X Chromium 3 kit and Illumina platform. The aim was to identify fat body cell types and assess differential gene expression between conditions.