title: whole genome sequence data of Andrena androfovea (new species) from Oklahoma
design: DNA was extracted from the tissue of three thoracic legs for each specimen. We used the Zymo Quick-DNA Miniprep Plus Kit (Zymo Research Corp.) according to the manufacturers protocol, except that we eluted the DNA of the spin column with 120 l of elution buffer. After DNA extraction, we quantified double-stranded DNA with a Qubit 3.0 fluormeter (Thermo Fisher Scientific, Inc.) and assessed fragmentation by running 2 l of each sample on a high sensitivity screening tape in a 4150 TapeStation System (Agilent Technologies, Inc.). We sheared a total of 50 ng to an approximate target fragment size of 300-500 bp with a BioRuptor 300 (Diagenode, Inc.). Following sonication, we prepared sequencing libraries for Illumina platforms using a KAPA HyperPrep Kit (F. Hoffmann-La Roche, Ltd.). We followed the protocol outlined in Branstetter et al. (2021), which starts with a purification step of the input DNA using magnetic beads at a 3:1 ratio (3.0x SPRI). The libraries were dual-indexed (Glenn et al., 2019) and we used the 2x TaKaRa Ex Premier DNA Polymerase mastermix (Takara Bio USA, Inc.) instead of the KAPA kits PCR mastermix. We ran a total of 15 PCR cycles, pooled the samples at equimolar concentrations and size selected the pool to a fragment distribution of 250-650 bp with a BluePippin device (Sage Science, Inc.). Sequencing was carried out on an Illumina NovaSeq X Plus sequencer and a 10B flow cell by Novogene Co., Ltd.