title: RNA-Seq of mus musculus: polyclonal Naive CD4 T cells from SLO
design: Total RNA was extracted from physically sorted CD4+ T cell populations using a QIAGEN RNeasy Kit according to the manufacturers instructions. Purified RNA quality control (Agilent sizing and RiboGreen quantification), library creation (Takara SMARTer Stranded Total RNA-seq Kit v2  Pico Input Mammalian), and sequencing (Illumina NovaSeq SP flow cell 2x50-bp paired-end run) were carried out at the University of Minnesota Genomics Center.