title: legumes comparative transcriptomics
design: we chose comparative transcriptomics to try to understand why the two species differed in nitrogen fixation. To obtain a more comprehensive transcriptome, we selected various tissue types from two different plants, including leaves, stems, root systems, and root nodules. Efforts were made to ensure that the sampling locations across the two plant species corresponded and had consistent growth patterns. Root nodules that were developmentally mature and had a single weight greater than 0.005 g were chosen as the material for sequencing.