title: metatranscriptome of iron microbial mat: stream at the Savannah River Site
design: An Illumina Low Input (RNA) library was constructed and sequenced 2x151 using the Illumina NovaSeq platform. Raw reads were processed with BBDuk (version 39.01) to remove contaminants, trim adapter sequences, remove homopolymer tails with 5 Gs, and right quality trim reads where quality drops to 0. BBDuk was also used to remove reads that contained 1 or more 'N' bases, had an average quality score below 10, or had a minimum length <51 bp. Reads mapped with BBMap to contaminants (human, cat, dog, mouse, common microbial contaminants, ribosomal RNA, or known spike-ins) were also removed