title: Zhendan958_V15_Anther_CK3
design: The RNA of maize anthers was isolated and purified using TRIzol (Thermofisher, 15596018) according to the operation protocol provided by the manufacturer. Then, the total RNA was quality-controlled for quantity, purity, and integrity. The mRNA with PolyA (polyadenylic acid) was specifically captured using oligo (dT) magnetic beads (Dynabeads Oligo, cat.25-61005, Thermo Fisher, USA) through two rounds of purification. The captured mRNA was fragmented at high temperature, reverse-transcribed into cDNA, and followed by second-strand synthesis to generate double-stranded DNA. Meanwhile, dUTP Solution was incorporated to fill the double-stranded DNA ends, add an A base to each end, screen and purify the fragments. The second strand was digested with UDG, followed by PCR to generate a strand-specific library (~300 bp)