title: scRNA-seq of mus musculus: adult skull/dura ZsGreen
design: Single-nucleus RNA-seq libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3 v3.1 (GEM-X Flex) assay. Sample multiplexing was performed using the 10x Genomics CellPlex Kit for Multiplexing (CG000391). Nuclei from different samples were pooled prior to GEM generation. Sequencing was performed on an Illumina NovaSeq X Plus (PE150, 2 lanes). Demultiplexing was performed using Cell Ranger Multi v6.1.2. Library QC and sequencing were conducted by Novogene.