title: metagenome: fermentation broth
design: Microbial genomic DNA was extracted using the MagicPure Size Selection DNA Beads Kit (Transgen, Beijing, China) according to the manufacturers protocol. The integrity of genomic DNA was assessed via agarose gel electrophoresis, and the concentration and quality of genomic DNA were determined using the Qubit (Invitrogen Qubit3.0 Spectrophotometer, Thermo Fisher Scientific, USA). DNA libraries were prepared using a ligation-dependent method with a DNA library preparation kit (Genesky, China). The 500-600 bp range of the sequencing library was size-selected and recovered using MagicPure Size Selection DNA Beads (Transgen, Beijing). The libraries were sequenced on NovaSeq 6000 platform (Illumina USA) and generated 10G bps of 2154 bp pair-end reads.