title: RNA-seq of C. osculatum (s.s.) third larval stage
design: Following the manufacturers protocol, the cDNA library was prepared using TruSeq Stranded mRNA kit (Illumina, San Diego, USA). The size- fractionated cDNA was end-repaired and adaptor-ligated according to the manufacturers protocol (Illumina). Ligated products of 200 bp were excised from agarose gels and PCR amplified.