title: Single-cell RNA sequencing of human fetal heart tissue
design: Flash-frozen human fetal heart tissues from a single donor were used to prepare the library. Nuclei were isolated through mechanical homogenization with a Dounce tissue grinder and purified with an iodixanol density gradient. Chromatin accessibility (ATAC-seq) libraries were constructed using Tn5 transposition as the library selection strategy, and RNA libraries were prepared for gene expression profiling. Library construction was performed using the 10x Genomics Chromium Single Cell Multiome ATAC + Gene Expression kit (CG000338 Rev F), the Chromium Next GEM Chip J Single Cell Kit (PN-1000234), and the Chromium Single Cell Multiome ATAC + Gene Expression Reagent Bundle (PN-1000283). Libraries were indexed with Single Index Kit N Set A (PN-1000212) for scATAC-seq and Dual Index Kit TT Set A (PN-1000215) for snRNA-seq.