title: eccDNA of extracellular vesicles derived from human dermal fibroblasts:donor2
design: DNA was isolated using a DNA Plasmid Isolation Kit, then subjected to treatment with endonuclease MssI, followed by treatment with ATP-dependent Exonuclease V. Samples were then subjected to rolling circle amplification (RCA), using the Phi29 DNA polymerase. Following RCA, samples were carried forward to sequencing library preparation, following Oxford Nanopores LSK-109 and NBD104 preparation protocol, and sequenced on the MinIon Mk1B device (Oxford Nanopore Technologies,UK).