title: bacterial community analysis of sediments from cold seep of cauvery Mannar basin
design: Amplicon library was made using Nexetra XT index Kit (Illumina Inc.) according to the 16s rRNA gene metagenomic sequencing library preparation protocol. Then, the V3-V4 region of the 16s rRNA gene was amplified using the following primers: forward primer: 5'-GCCTACGGGNGGCWGCAG-3 and reverse primer: 5'-ACTACHVGGGTATCTAATCC-3. Amplicons that have passed the QC with the Illumina adaptors were then amplified using i5 and i7 primers as per the standard Illumina protocol which is then followed by analysis of the amplified amplicons on 4200 Tape Station system (Agilent Technologies) using D1000 Screen tape.