title: Bulk whole-genome sequencing from Aedes aegypti: refractory (0 L3 Dirofilaria immitis)
design: Mosquitoes pertaining to each bulk population (refractory or susceptible) were homogenized by bead beating and DNA was extracted with the Quick-DNA Tissue/Insect Miniprep Kit. Recovered gDNA was prepared for sequencing according to the Celero PCR Workflow with Enzymatic Fragmentation. Quality and quantity of finished libraries were assesed using an Agilent Tapestation and Qubit dsDNA HS Assay Kit prior to sequencing. Two separate sequencing runs were completed to increase coverage of each library.