title: Target capture sequencing for T-DNA insertion characterization in potato
design: For NGS library prep, samples normalized to 200 ng of fragmented DNA were end-repaired, ligated with adapters (NEBNext Ultra II DNA Library Prep Kit for Illumina, cat. E7645, NEB, Ipswich, MA); dual-indexed with NEBNext multiplex oligos for Illumina set I & set II (New England Biolabs catalog no. E7334S and E7500S); size selected and cleaned up using Agencourt AMPure XP beads (cat. A63881, Beckman Coulter, Brea, CA).