title: Illumina sequencing of Pseudovibrio ascidiaceicola 5337
design: Pseudovibrio ascidiaceicola strain 5337 was cultured from the gut of Ciona robusta harvested from Mission Bay, San Diego. The species was isolated by filtering the combined suspension from homogenizing the gut of five animals and plating an aliquot of the filtrate onto BD Difco Marine Agar 2216 (Franklin Lakes, NJ, USA). One of the isolated and purified colonies was incubated overnight at room temperature (20-22C) in Marine Broth with continuous shaking at 120 rpm. DNA extraction was performed on 1 mL of this culture (OD600>=1.2) using the Purelink Microbiome DNA Extraction Kit (Invitrogen, Waltham, MA, USA). Purified DNA samples were sequenced on the Illumina MiSeq 2250 bp platform (San Diego, CA, USA).