title: WGBS LRV_0_1 Daphnia magna control conditions
design: Directional bisulfite libraries were prepared by fragmenting genomic DNA to 100-300 bp by sonication. DNA-end repair was then carried out along with ligation of methylated sequencing adaptors. The ZYMO EZ DNA Methylation-Gold kit was used for bisulfite treatment with subsequent desaltation, size selection, PCR amplification and final size selection before sequencing. A 1% unmethylated lambda spike was included in the sequencing run to assess bisulfite conversion rates in downstream analyses.