title: Rhizosphere metagenome
design: Rhizosphere isolation was performed by sonicating the roots in Falcon tubes for 30 seconds. DNA extraction from microbial communities present in rhizosphere samples was performed using the PowerSoil DNA kit (Qiagen), following the manufacturer's instructions. The library was constructed using a fragment sequencing approach targeting the V3 and V4 regions of the 16S rRNA gene.