title: RNASeq of MCF7 cells: siCTRL rep1
design: MCF7 cell line was transfected with sip53 or siCTRL. After 72 h cells were collected and RNA was isolated with TRI Reagent. mRNA was isolated with NEBNext Poly(A) mRNA Magnetic Isolation Module. Next mRNA was converted do cDNA using NEBNext Ultra II RNA First Strand Synthesis Module and NEBNext Ultra II Non Directional RNA Second Strand Module according to manufacturer instructions. The purified cDNA was used for RNAseq library preparation using MGIEasy PCR Free DNA Library Prep Set according to the protocol. Sequencing was performed using DNBSEQ G400.