title: shotgun metagenomics sequencing of human stool
design: The DNA library was generated using NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) for Illumina with parameters as followed: 500 ng input DNA and 5 min at 37C for fragmentation; > 550-bp DNA fragments for size selection; primers from NEBNext Multiplex Oligos for Illumina Kit (New England Biolabs, Ipswich, MA, USA) for barcoding. The libraries were sequenced on the Illumina NovaSeq (2 × 150 bp).