title: RNA-seq of pooled developmental stages comprising 4 larvae of different sizes, 2 pupae, and 5 adult workers.
design: RNA extraction with Trizol followed by enrichment of mRNA with NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490) with the NEBNext Ultra ll Directional RNA library Prep Kit for Illumina (#E7760S) according to the manufacturers instructions.