title: V3-V4 region of the 16S rRNA gene in mouse fecal sample (ABX 0.5X Week 6 Rep 4)
design: Six-week-old male C57BL/6Narl mice were randomly assigned to three experimental groups based on treatment: (1) Control (CON), which received standard drinking water; (2) Abx_0.2X, which received drinking water supplemented with a 0.2X concentration of an antibiotic cocktail; and (3) Abx_0.5X, which received a 0.5X concentration of the same antibiotic cocktail. The 1X antibiotic cocktail consisted of ampicillin (1 g/L), metronidazole (1 g/L), vancomycin (0.5 g/L), and neomycin (1 g/L). The treatment duration was 6 weeks, during which all groups were monitored for body weight, and fecal samples were collected weekly. DNA was extracted from the fecal samples using the QIAamp PowerFecal Pro DNA Kit (QIAGEN, Netherlands) according to the manufacturers instructions. The V3-V4 hypervariable region of the 16S rRNA gene was amplified using universal primers (forward: 5-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3 and reverse: 5-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3). The PCR products were purified, quantified, and sequenced using the Illumina MiSeq platform (2  300 bp paired-end reads). This sequencing data was used to analyze the composition and diversity of the gut microbiota under different antibiotic regimens.