title: WGS of Francisella tularensis : FSC237clpB lineage A2 passage 4
design: Sequencing libraries were prepared from 100ng DNA using the TruSeq Nano DNA sample preparation kit (cat# FC-121-4001/4002, Illumina Inc.) targeting an insert size of 350bp. The library preparation was performed according to the manufacturers' instructions (guid#15041110, revC). A sequencing library for the phage PhiX was included as 1% spike-in in the sequencing run. Sequencing performed using HiSeq2500, PE 125 bp read length, and v4 sequencing chemistry.