title: 16S rRNA V1-V2 region metagenome: adult forager gut
design: Microbial genomic DNA was extracted from the honey bee gastrointestinal tracts using the DNeasy UltraClean Microbial Kit (Qiagen). The hypervariable V1-V2 region of the 16S rRNA gene from each sample was amplified using 27F and 338R primers barcoded with unique octameric multiplex identifiers using a dual indexing approach on an Illumina MiSeq sequencing platform.