title: RNA-seq of homo sapiens: primary human monocytes
design: For RNA sequencing experiments, all samples were treated in triplicate and libraries were generated using the Kapa mRNA HyperPrep Kit, and they were sequenced on a NextSeq1000/2000 using NextSeq 1000/2000 P2 Reagents (200 Cycles) with dual indexing. Adapters and low-quality bases were trimmed from paired-end raw reads using Trimmomatic (v0.39), followed by alignment to the human genome (GRCh38) using STAR aligner (v2.7.10b). Gene expression was quantified using RSEM (v1.2.29) to generate read counts. Read counts were analyzed using DESeq2 (Love et al., 2014) and gene expression pathways analyzed using DAVID(Huang et al., 2009) and cluster profiler (Yu et al., 2012)