title: Hi-C seq of the Cibotium barometz: young fronds
design: DNA extraction and Hi-C library preparation High-molecular-weight DNA was extracted from young fronds of Cibotium barometz using a modified CTAB method . Fresh tissue was ground in liquid nitrogen, and DNA was isolated using CTAB buffer, followed by chloroform extraction and isopropanol precipitation. The quality and quantity of extracted DNA were assessed using Nanodrop spectrophotometry and agarose gel electrophoresis. Hi-C libraries were prepared following the in situ Hi-C protocol with minor modifications. Briefly, chromatin was fixed with formaldehyde, digested with HindIII restriction enzyme, filled in with biotinylated nucleotides, and proximity ligated. After reversal of crosslinks, the DNA was purified and sheared to 300-500 bp fragments. Biotinylated fragments were captured on streptavidin beads, and sequencing adapters were added to construct the final Hi-C libraries.Sequencing Hi-C libraries were sequenced on an Illumina NovaSeq 6000 platform using 150 bp paired-end reads.