title: Short-read, paired-end DNA sequencing of unclassified bacterial virus, phage lysate DNA
design: Phage lysates were collected from bacterial lawns exhibiting near-confluent lysis after infection by pure phage isolates. Top agar was scraped into a glass universal bottle containing 3 ml of LB and homogenized using a sterile pipette. The suspension was then centrifuged (4500 x g, 4 , 20 min). The obtained supernatants were filtered through a 0.22 m filter and stored in bijoux bottles at 4 . Phage titer was estimated by counting the number of plaques obtained from phage lysate dilutions, plated out. Genomic DNA of phages was obtained from 450 L of high-titer phage lysates (~1010 PFU/mL) using a standard phenol/chloroform method as described by Chen et al. (2017).