title: PhaseII Replicate 2
design: Total RNA was extracted using an RNeasy Mini Kit (QIAGEN, 74104) according to the manufacturer's instructions. RNA was treated with DNAse I, eluted in 50 L RNase-free water and quantified using a Qubit 4 fluorometer (Invitrogen) or NanoDrop One (ThermoScientific). A total of 1 g RNA was reverse transcribed using the SuperScript III First-Strand Synthesis System for RT-PCR (Invitrogen, 18080-051) according to the manufacturer's instructions and 2 L was used in PCR reactions.  Amplification of cDNA was carried out with Ex Taq DNA polymerase (Takara, RR001A).