title: RNA-Seq of Saccharomyces paradoxus: zero Ty1 #2 YPD + HU
design: RNA was extracted using the RNAqueous Phenol-free Total RNA Isolation kit, poly(A) RNA was purified using two rounds of oligo(dT) magnetic beads, and fragmented at high temperature in divalent cation buffer. DNA library was constructed by first strand cDNA synthesis, second strand synthesis with dUTP, end repair, 3' adenylation, adapter ligation, UDG treatment, and PCR.