title: Nanopore whole genome sequencing of muscle sample from Vietnamese bos gaurus
design: Total DNA was extracted from the muscle sample using the Gentra Puregene Tissue kit (QIAGEN, Germany), following the manufacturers instructions. DNA quantity and quality were assessed using a Qubit 4 Fluorometer (Thermo Scientific, USA) and a NanoDrop 1000 Spectrophotometer (Thermo Scientific, Wilmington, DE).Sequencing libraries were prepared using the Ligation Sequencing Kit v10 (SQK-LSK110) from Oxford Nanopore Technologies (ONT, UK) with modifications as described in Hayes et al. 2021 (9), and sequenced on a PromethION device with R9 flow cells (ONT, UK).