title: 16S RNA-Seq of AD Bacterial metagenome
design: Genomic DNA was extracted and purified from all the cells of each sample, then all 16S ribosomal RNA genes were amplified by PCR using oligonucleotides targeting conserved regions common to all microorganisms (16S-RNAr rgion V4-V5). The sequence of each obtained fragment was then determined by high-throughput sequencing.