title: Isolation of Pseudomonas aeruginosa phage genomes from Sewage
design: Library preparation was performed using QIASeq FX DNA Library Preparation protocol(Cat#180475) by following manufacturers instructions. About 50 ng of Qubit quantified DNAwas enzymatically fragmented, end-repaired and A-tailed in a one-tube reaction using the FXEnzyme Mix provided in the QIASeq FX DNA kit. The end-repaired and adenylated fragmentswere subjected to adapter ligation, whereby index-incorporated Illumina adapter was ligated, togenerate sequencing library. The library was subjected to 6 cycles of Indexing-PCR (InitialDenaturation at 98C for 2 min, cycling (98C for 20sec, 60C for 30sec, 72C for 30sec) andfinal extension at 72C for 1min) to enrich the adapter-tagged fragments. Finally, the amplified library was purified using Cambrian Bioworks CamSelect NGS(#CBWD010) followed by library quality control check. Illumina-compatible sequencinglibraries were quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and itsfragment size distribution was analyzed on Agilent 4150 TapeStation.