title: 16s rRNA of AMD water samples
design: The water samples to be tested were mixed, 50 mL was measured, and the sterilized 0.22 m filter membrane was used for suction filtration, and the bacteria-enriched filter membrane was stored in a sterilized 10 mL centrifuge tube, and the above operations were repeated three times, sealed and placed in a -80 C freezer, and handed over to Shanghai Biotechnology Co., Ltd. for high-throughput sequencing of 18s rRNA gene fragments.