title: Dovetail's Omni-C sequencing data from Ursus americanus: blood sample from adult male L20-20
design: The Omni-C library was prepared using DovetailTM Omni-CTM Kit (Dovetail Genomics, CA) according to the manufacturers protocol with slight modifications. Briefly, chromatin was fixed in place in the nucleus. Fixed chromatin was digested with DNase I then extracted. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA purified from proteins. Purified DNA was treated to remove biotin that was not internal to ligated fragments. An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor. Biotin-containing fragments were then captured using streptavidin beads. The post capture product was split into two replicates prior to PCR enrichment to preserve library complexity with each replicate receiving unique dual indices. The library was sequenced on an Illumina NovaSeq platform to generate approximately 100 million 2 x 150 bp read pairs per GB genome size.