title: 16S rRNA-seq of mus musculus: adult feces
design: fecal samples were collected into autoclaved Eppendorf tubes and snap frozen at -80C. DNA extraction from fecal samples was performed using QIAamp PowerFecal Pro DNA Kit. Barcoded dual-index universal bacterial primers 563F and 926R were used to PCR-amplify the V4-V5 hypervariable region of the 16S rRNA gene. Illumina compatible libraries were generated using the Qiagen QIASeq 1-step amplicon kit and sequencing was performed on the Illumina MiSeq platform using 2x250 paired-end reads.