title: ITS3-4 Sequencing: Cat Rectal Microbiome
design: DNA was extracted using the QIAamp PowerFecal Pro Kit. Fungal ITS3_4 was amplified (forward 5-GCATCGATGAAGAACGCAGC-3 and reverse 5-TCCTCCGCTTATTGATATGC-3), primer dimers removed, and DNA evaluated on a Qubit and Fragment Analyzer. The pool was and loaded onto the MiSeq V2 Nano . The libraries were sequenced from both ends to a total read length of 250nt from each end. The raw fastq files were converted into demultiplexed compressed fastq files using bcl2fastq 2.20.