title: WGS of tobacco-planting soil
design: 30 to 50 ng of DNA was sonicated to a 100 - 800 bp size range using the Covaris ME220 (Covaris, USA). DNA fragments were subsequently end repaired and 3-adenylated before Illumina adapters were added by using the NEBNext Sample Reagent Set (New England Biolabs). Ligation products were purified by Agencourt AMPure XP Nucleic Acid Purification Kit (Beckman Coulter, Germany). High-quality libraries were constructed using the Next Ultra IIDNA Library Preparation Kit (NEB, UK), and DNA fragments were PCR-amplified using Next Q5 Hot Start HiFi PCR PreMix (NEB, UK). After library profile analysis using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA) and qPCR quantification (MxPro, Agilent Technologies, USA), each library was sequenced using 101 base-length read chemistry in a paired-end flow cell on Illumina sequencing machines (Illumina, USA).