title: Whole genome sequencing:Human gut derived probiotics 3
design: Genomic DNA was isolated with NucleoSpin Microbial DNA Mini kit according to the manufacturer`s protocol. Isolated genomic DNA was quantified with Equalbit 1x dsDNA HS Assay Kit (Vazyme Biotech Co., Nanjing, China) and quality assessed by Agarose Gel. Library preparation was performed using KAPA Hyper Prep kit with PCR (Roche, Basel, Switzerland) per manufacturers recommendations. Library quality and quantity were assessed with Qubit 2.0 DNA HS Assay (ThermoFisher, Massachusetts, USA), Tapestation High Sensitivity D1000 Assay (Agilent Technologies, California, USA), and QuantStudio  5 System (Applied Biosystems, California, USA). Illumina 8-nt dual-indices were used. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina NovaSeqX Plus platform (Illumina, California, USA) with a read length configuration of 150 PE for 6.667M PE reads (3.335 in each direction) per sample.