title: Human Gut Metagenome
design: DNA library was prepared with the Ligation sequencing kit (SQK-LSK109) (Oxford Nanopore Technologies (ONT), Oxford, UK), then loaded onto a R9.4.1 MinION flow cell (FLO-MIN106) and sequenced on the ONT MinION Mk1B device (MIN-101B). Basecalling and demultiplexing of sequence reads was performed with Guppy v4.2.2 and with assistance by MinKNOW GUI v4.1.22. Raw sequencing reads were stored in FastQ format for further computational analysis.