title: Single-cell transcriptomes of two Protaspididae (Cercozoa)
design: Single cells of two Protaspididae were isolated by micropipetting from ocean surface water on Quadra Island (collected with a hand-tow plankton net). After imaging, cells were washed 3-4 times in sterile seawater, deposited in a PCR tube and lysed using 3-4 freeze-thaw cycles. This lysate was then used as input into SmartSeq2 (Picelli et al 2014) to generate single-cell transcriptomes using 24 PCR amplification cycles.