title: RNA-Seq protoplasts OE GATA4 for 8 hours, repeat 3
design: 10 g of a plasmid containing GATA TF or GFP driven by the double 35S promoter was transformed into 200,000 Arabidopsis leaf protoplasts. Each construct was transformed in two replicates into two independent protoplast preparations, resulting in four replicates per construct. After 8 h incubation at 25C under constant light (85 mol/m2/s), samples were centrifuged at 450xg for 5 min at room temperature, and pellets were snap frozen and stored at -70C. RNA purified from these samples was used to construct SMART-Seq3 libraries in multiple technical replicates per library and sequenced on Illumina NovaSeq X.