title: Fecal microbiome of IBS mouse model
design: 16S rRNA (V3-V4) sequencing of fecal microbiota from IBS-model mice (TNBS+CRS, C57BL/6J). DNA was amplified with 341F/805R primers and libraries were prepared using the Illumina 16S Metagenomic Sequencing Library Kit. Paired-end sequencing (2250 bp) was performed on Illumina NovaSeq 6000. Data processed with QIIME2 (DADA2, Silva v138).