title: RNA-Seq of leavesMedicago sativa L.
design: RNA was extracted from the leaves using Trizol regent (Invitrogen, Carlsbad, CA, USA). Evaluated quantity was using Nanodrop 2000 (NanoDrop, Wilmington, DE, USA), and detected integrity was by Agilent 2100 Bioanalyzer (Agilent Technologies, Inc, Santa Clara, CA, USA). RNA samples with a ratio of 260/280nm greater than 1.8 and an RNA integrity number (RIN) greater than 7 were selected for further processes. Sequencing of cDNA library using the Illumina HiSeq 2000 platform.