title: scRNA sequencing of human-derived cardiac organoids: ORG_L single cells
design: The Chromium Next GEM Single Cell 3' Reagent Kit v3.1 was used to generate libraries by encapsulating single cells in bead-in-emulsion (GEM) droplets. Each GEM contains a barcoded bead with oligonucleotides, allowing unique labelling of cDNA synthesised from the mRNA of each cell. The process involves: (i) loading cell suspensions onto the Chromium instrument, (ii) reverse transcription GEM generation within each droplet, (iii) barcoded cDNA amplification after emulsion breakage, (iv) library construction by fragmentation, adapter ligation and indexing.