title: Mosquito #33, DENV-exposed, collected at 11DPF - carcass
design: RNA was extracted from a single mosquito carcass with TRIzol and the NucleoSpin RNA kit (Macherey-Nagel). Libraries were rRNA-depleted with the Zymo-Seq RiboFree kit (Zymo Research) and indexed with the Illumina Stranded mRNA kit. Libraries were sequenced on an Illumina instrument and yielded a minimum output of 30mln 2x100bp reads.