title: Development and characterization of Fluorescent Protein-Tagged Vibrio harveyi strains as a versatile tool for studying infection dynamics and strain interactions.
design: DNA was fragmented and subjected to size selection, followed by end-repair and the addition of "A" tails. Adapters were then ligated to the fragments, and a second round of size selection was performed. This was followed by PCR amplification to enrich the library, and comprehensive quality control (QC) was conducted to ensure the library's integrity. Low-quality reads and adapter-contaminated sequences were filtered from the raw data to ensure high-quality output. Data processing, including contamination removal and validation of usable reads, was carried out using SOAPnuke