title: 16s of biofilm in untreated plates in summer replicate 2
design: For the generation of amplicons and library preparation of prokaryotes, 16S rRNA gene was amplified, respectively. PCR was performed using the following primer sets: 16S rRNA (V3-V4 region) Bakt_341F 5-CCTACGGGNGGCWGCAG-3and Bakt_805R 5-GACTACHVGGGTATCTAATCC-3.