title: F1
design: "Genomic DNA was extracted using the Cetyltrimethyl Ammonium Bromide (CTAB) method with minor modifications to improve yield and purity. The DNA concentration, quality, and integrity were assessed using a Qubit Fluorometer (Invitrogen, USA) and a NanoDrop Spectrophotometer (Thermo Scientific, USA). Sequencing libraries were prepared using the Illumina TruSeq DNA Sample Preparation Kit and the Template Prep Kit (Pacific Biosciences, USA), following the respective manufacturers' protocols. Genomic DNA was fragmented using a Covaris system to generate fragments approximately 300-350 bp in size. The DNA was subjected to double-end repair, an 'A' base was added to the 3' ends, and sequencing adapters were ligated. Adapter-ligated fragments were enriched through PCR amplification and purified using magnetic beads. Library quality and fragment size distribution were verified using an Agilent Bioanalyzer 2100 and quantified using the Quant-iT PicoGreen dsDNA Assay Kit.