title: WGS of Trichophyton indotineae
design: Isolated cultures of each sample were diluted in 1mL sterile saline and submitted to DNA extraction using the Power Soil Pro DNA Isolation Kit (Qiagen) according to the manufacturers instructions. The library preparation for NGS (Next Generation Sequencing) were performed using Nextera XT kit (Illumina). Briefly, the total DNA of each sample was tagmented by transposomes and submitted to few cycles of amplification for dual index adapters ligation. The libraries were then purified, qualified by Tapestation (Agilent) and quantified by Qubit spectrophotometer (Thermofisher). The resulting libraries were pooled at equimolar concentrations and submitted to 2x 150bp paired end reads using Illumina NextSeq 550 sequencing platform (Illumina).