title: Nanopore sequence of Pseudooceanicola nitratireducens NEH7
design: High-molecular-weight genomic DNA was extracted using the Monarch HMW DNA Extraction Kit (#T3010), following the manufacturers protocol. Libraries were prepared using the Oxford Nanopore Rapid Barcoding Kit 24 (SQK-RBK114.24) and sequenced on a MinION R10.4.1 flow cell (FLO-MIN114). Basecalling was performed with Dorado (via MinKNOW v24.11), and adapter trimming was performed using Porechop_ABI v0.5.0. Long reads were quality- and length-filtered using nanoq v0.10.0 (--min_length 500, -q 8 -E 50)