title: Isolation of Pseudomonas aeruginosa phage genomes from Sewage
design: 3 DNA sample was further taken for DNA library preparation using Twist EF Library Prep Kit for Illumina (ref_100572 Lot no._2500002597). First, the DNA was fragmented in desired size then end repaired and mono-adenylated at 3 end in a single enzymatic reaction. Next,adapters are ligated to the DNA fragments in a T4-DNA ligase-based reaction. Following ligation, to make it available as a substrate for PCR based indexing in the next step. During PCR, barcodes were incorporated using unique primers for each of the samples thereby enabling multiplexing (See, Table1 : List of indexes used for respective samples). All Prepared libraries were checked for fragment distribution by 5300 Fragment analyzer system.