title: GFP8
design: To analyze the gut microbiota, fecal samples from rats were collected, and bacterial ge-nomic DNA was extracted using the E.Z.N.A. Stool DNA Kit (OMEGA, Norcross, GA, USA). The extracted genomic DNA was quantified and used as a template to amplify the hypervariable regions (V3/V4) of the 16S rRNA gene. The resulting PCR products were purified, and their concentrations were normalized to achieve equimolar ratios. Paired-end sequencing libraries were constructed using the TruSeq DNA Sample Prep Kit (Illumina, San Diego, USA), followed by high-throughput sequencing performed on an Illumina MiSeq platform at Majorbio Technology Co., Ltd. (Shanghai, China)