title: RNA-seq of zebrafish hearts: tbx2b mutant
design: Hearts were isolated from 48 hpf zebrafish embryos based on heart looping phenotype. Pooled heart tissues were homogenized in TRI Reagent, and total RNA was extracted using the Direct-zol RNA MicroPrep kit (Zymo Research). RNA concentration and integrity were assessed by NanoDrop and Fragment Analyzer before library preparation. RNA-seq libraries were sequenced on the Illumina platform using 150 bp paired-end reads.