title: CK3
design: Total RNA was extracted using the TIANGEN RNA Plant Plus Reagent kit. RNA concentration and purity were measured using a Nanodrop 2000 (OD260/280 ratio between 1.82.2, concentration  30 ng/L). RNA quality was assessed using an Agilent 2100 Bioanalyzer (RIN > 6.5), and RNA integrity was verified using a LabChip GX. Qualified samples were used to construct cDNA libraries with the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina. Sequencing was performed on the Illumina NovaSeq 6000 platform in PE150 mode.