title: RNA-seq of lithobates pipiens skin NC101
design: The Qiagen QIAamp PowerFecal Pro DNA Isolation Kit was used to extract bacterial DNA from GI tissue, skin swabs, and natural and autoclaved pond water samples, following manufacturer's instructions. Extracted DNA was stored in sterile microcentrifuge tubes at -80C. For sequencing services, frozen DNA was shipped on dry ice to the University of Illinois at Chicagos Genome Research Core (UIC GRC). At the UIC GRC, libraries were prepared via amplification of the V4 region of the 16S rRNA gene using 515F and 806R primers on an Illumina MiSeq platform (2x300 paired end reads).