title: BB5
design: The V4-V5 hypervariable regions of the 16S rRNA gene (amplicon size ~480 bp) were amplified from soil DNA using universal bacterial primers 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 907R (5'-CCGTCAATTCMTTTRAGTTT-3'). Paired-end libraries were constructed with Illumina TruSeq Nano DNA LT Kit and sequenced on the Illumina NovaSeq 6000 platform with 2250 bp chemistry (NovaSeq 6000 SP Reagent Kit).