title: Draft genome sequence of Petromyzon marinus: white blood cells
design: A total of 1 g of purified DNA was used for library preparation with the Ligation Sequencing DNA V14 kit (SQK-LSK114, Oxford Nanopore Technologies) and the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB, E7180S), following the manufacturers protocols. The prepared library was applied to a PromethION Flow Cell (R10.4.1, FLO-PRO114M, Oxford Nanopore Technologies) and sequenced using the PromethION 2 Solo (Oxford Nanopore Technologies) for approximately 24 hours. Sequencing was monitored using MinKNOW (version 23.11.7). Variant calling was performed using Dorado (version 0.9.1) in super accuracy mode with the basecalling model dna_r10.4.1_e8.2_400bps_sup@v5.0.0. The file format was converted from BAM to FASTQ using Samtools (version 1.21)