title: =_xlfn.CONCAT("RADseq of Yellowtail Rockfish: ",B214)
design: Genomic DNA was extracted using the Nexttec DNA isolation kit following the manufacturers protocol. DNA concentration was normalized to 125 ng in 10 L of molecular-grade water. Restriction site-associated DNA sequencing (RADseq) libraries were prepared using a version of the Ali et al., (2016) protocol without the targeted bait capture step, referred to in the literature as BestRAD. Each library was assessed for quality on a 1% agarose gel and a Bioanalyzer DNA 1000 kit. Libraries were pooled in equimolar amounts and sequenced on a NovaSeq (paired-end, 116 bp or 150 bp) at the University of Oregon, either an S4 or SP run type.