title: HAEC_rna_oxldl2_batch1
design: HAECs were cultured and harvested at passage 4-5. Total RNA was purified using RNeasy Micro Kit (Qiagen) after TRIzol and chloroform extraction according to the manufacturers protocol. The quality of RNA samples was analysed using a TapeStation 2200 with High Sensitivity RNA ScreenTape (Agilent Technologies). Samples with high integrity (RIN score>9) were submitted directly to the Oxford Genomics Centre for subsequent PolyA-enrichment and TruSeq library preparation. For each sample, 1 ug of total RNA was submitted and the final library was sequenced on a NovaSeq 6000 device with a target depth of 37.5 million pairs of 150 bp reads.