title: RNA-seq of mouse melanoma macrphage(PBS)
design: For analysing gene expression in early infiltrated macrophages in tumors, 6-8 weeks old WT mice of either sex were subcutaneously implanted at 0hr with B16F10 murine melanoma cells, 5million/mouse. Treatments (PBS/RHP) were administered intraperitoneally at 0hr and 24 hrs. Animals were sacrificed 40hrs after tumor implantation. Tumor infiltrating macrophages ((Live CD45+DUMP-(Ly6G-CD3-CD19-CD11c-)CD11b+F4/80+)) were FACS sorted for RNA-Seq. Samples from 4-5 mice were pooled to make a single sample and subjected to sorting.(CD45+DUMP(Ly6GCD19-CD3-CD11c-)- CD11b+F4/80+ cells were FACS sorted for bulk RNA sequencing.