title: human bladder metagenome
design: DNA from 32 FFPE bladder cancer samples (Table S1) was extracted using a MagPure Soil DNA LQ Kit. The V3V4 region of 16S rRNA was amplified with F343/R798 primers and sequenced on an Illumina NovaSeq 6000 (250 bp paired-end reads). Data were processed via QIIME2: adapter trimming, denoising, chimera removal, and annotation against the Silva database (RRID:SCR_006423) (v138). Differential abundance was analyzed by ANOVA/Kruskal-Wallis and LEfSe (RRID:SCR_014609).