title: 16S rRNA amplicon sequencing of South Pacific microbes from GO-SHIP P18 line
design: RNA was extracted using the NucleoMag RNA kit (Macherey-Nagel). The V4-V5 region of 16S was targeted with the degenerate primer pair F515 (GTGNCAGCMGCCG CGGTAA) and R926 (CCGYCAATTYMTTTRAGTTT).