title: Virome of Lynx rufus: feces
design: We used the Twist Bioscience Comprehensive Viral Research Panel for targeted enrichment of animal-infecting viruses. We used DNA and RNA (co-extracted) from scat and buccal swabs of bobcats as input for the Twist Total Nucleic Acids Library Preparation EF Kit 2.0 for Viral Pathogen Detection and Characterization protocol  (Twist Bioscience). The libraries were then subjected to hybridization using the Twist Target Enrichment Standard Hybridization v2 Protocol (Twist Bioscience) and 75 bp PE sequencing on a NextSeq 500.