title: Subsample of Basque Lake: Control_neg1 Forward read
design: Genomic DNA libraries were prepared using the Nextera XT Library Prep Kit (Illumina, San Diego, USA) and sequenced on an lllumina NovaSeq 6000 (Illumina, San Diego, USA) using a 250 bp paired end (Bankevich et al., 2012; Bolger et al., 2014).  Coverage of 53-fold was achieved, calculated using BWA, SAMtools (v0.1.19), and BEDTools genomecov (v2.2.7) with default settings (Li et al., 2009; Li and Durbin, 2009; Quinlan and Hall, 2010).