title: small RNA sequencing of Naegleria fowleri: extracellular vesicles
design: Extraction and purification of exosomal RNAs from isolated EVs via chromatography, addition of Illumina barcodes and amplification, library size-selection and PAGE purification, and library QC via Bioanalyzer. Following this, libraries were sequenced on an Illumina NextSeq instrument with 1x75 bp single-end reads at an approximate depth of 10-15 million reads per sample.