title: Nanopore sequencing of Wolbachia endosymbiont of Rhagoletis cingulata wCin2-SW: Wolbachia reads
design: An ONT LSK109 ligation library was prepared from HMW DNA from a single Rhagoletis fly and sequenced on a R9.4.1 MinION flowcell. Raw data was basecalled using the Guppy v6.01 with the SUP model and quality filtered (>Q8 and >500 bp). Reads were mapped to the assembled Wolbachia genome with minimap2 and unmapped reads were dropped with Samtools.