title: RNA-seq of ZF4 cells treated with arsenite and control
design: RNA sequencing was performed on zebrafish embryonic fibroblast (ZF4) cells to investigate the transcriptomic responses to arsenite exposure. Cells were treated with 20 M arsenite for 24 h and compared with untreated control cells. Three biological replicates were collected per group, and total RNA was extracted and sequenced using Illumina technology to identify differentially expressed genes and pathways involved in arsenite-induced cytotoxicity.