title: Lactobacillus crispatus MA line 61
design: The samples were normalized to target 100 ng of total input for each sample, and libraries were prepared using the Twist Biosciences kit (P/N:104207) following a miniaturized version of the manufacturers protocol. The libraries were quantitated and pooled for downstream processing. The pool quality is assessed using the Agilent Bioanalyzer and quantified using a qPCR-based method with the KAPA Library Quantification Kit (P/N: KK4873) and the QuantStudio 12K instrument. Prepared libraries were pooled in equimolar ratios, and the resulting pool was subjected to cluster generation using the NovaSeq 6000 System, following the manufacturer's protocols.