title: pacbio hifi data
design: PacBio HiFi sequencing was performed at the University of Washington USA, as previously described in (https://www.biorxiv.org/content/10.1101/2022.12.01.518658v2) with some modifications. Briefly, high-molecular-weight DNA was extracted from the flash-frozen peripheral blood samples using the QIAGEN MagAttract HMW DNA Kit (p/n 67563). After quality and quantity checks (Agilent FEMTO Pulse p/n M5330AA & FP-1002-0275, Denovix DS-11 FX, Thermo Fisher Q32854,) DNA was sheared on Megaruptor 3 (Diagenode B06010003 & E07010003) twice using settings 29/31 to obtain a peak size around 22 Kb. SMRTbell library preparation with the SMRTbell Express Template Prep Kit 2.0 (PacBio p/n 100-938-900) and size selection on Sage Science PippinHT (p/n HPE7510) were performed as previously described in https://www.biorxiv.org/content/10.1101/2022.12.01.518658v2. Next, sequencing was performed using 3-4 SMRT cells on the Sequel II platform with chemistry version 2.2, 30-hr movie times and 2 hr pre-extension (PacBio p/n 101-894-200)