title: HCT116 untreated, MicroC replicate 1
design: Micro-C libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7645) according to manufacturer instructions with a few modifications. The sequencing library was amplified by Kapa HiFi PCR enzyme with lowest possible cycles to reduce PCR duplicates. Library concentration, quality and fragment size were assessed by Qubit fluorometric quantification (Qubit dsDNA HS Assay Kit, InvitrogenTM Q32851) qPCR and Fragment analyzer. 20 multiplexed libraries were pooled and sequenced in 6 lanes on the Illumina NovaSeq sequencing platform (100-bp, paired-end reads)