title: RNA-Seq of  amoeba infected with bacteria
design: 5106 pure amoebae cells  infected with bacteria in the logarithmic phase, which were preserved in 1 mL of TRIzol reagent (Invitrogen, Carlsbad, USA) and frozen using liquid nitrogen. Majorbio Technology Co., Ltd (Shanghai, China) conducted the bioinformatics analysis using the Illumina NovaSeq 6000 platform (Illumina, CA, USA).