title: QR035Orchid fleck virus
design: The cDNA library was constructed using the Illumina TruSeq Stranded Total RNA Library Prep Plant kit (Illumina, Inc., San Diego, CA, USA) following the modified Illumina Ribo-Zero Total RNA recommendations. The quality and quantity of the unique dual-indexed cDNA libraries were analyzed using a 4200 TapeStation instrument (Agilent, Santa Clara, CA, USA), and Qubit Flex Fluorometer with dsDNA High Sensitivity kit. Pair-end sequencing was conducted using a NextSeq 550 system with 2  75 bp (V2) Hi-output sequencing reagent kits (Illumina, San Diego, CA, USA).