title: RNA-Seq of Chorispora bungeana: UV9h-3
design: The process begins by fragmenting purified mRNA into small pieces using divalent cations. These fragments are then converted into first-strand cDNA with reverse transcriptase and random primers. Second-strand cDNA is synthesized using DNA Polymerase I and RNase H. Adapters are ligated to the resulting cDNA fragments. The products are purified and enriched through PCR amplification. Finally, samples are pooled together to form a single-strand DNA circle (ssDNA circle), creating the final library.