title: Amplicon_culturomics from field-grown crop plants
design: Amplified the V4 region of the 16S rRNA gene using the V4_515F (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTGCCAGCMGCCGCGGTAA -3) and V4_R (5- GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC -3) primers of DNA extracted from barley spikes to compareautomated and manual library preparation methods.