title: 16S of arid soil microbial community: SAMP8-S1-16S_S83
design: DNA was extracted from 250 mg of soil using the DNeasy PowerSoil Pro Kit. T he variable V3 and V4 regions of the 16S rRNA gene were amplified for each sample using the primers Forward = 5'-CCTACGGGNGGCWGCAG-3 and Reverse = 5'-GACTACHVGGGTATCTAATCC-3 (Klindworth et al. 2013). Both primers were extended with Illumina overhang adaptors on their 5 end. Combined amplicon pools were sequenced in an Illumina MiSeq platform using the v3-600 cycle kit.