title: HEK293 nutlin-treated, RNA-seq replicate 2
design: RNA-seq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat. E7760S). Library concentration, quality and fragment size were assessed by Qubit fluorometric quantification (Qubit dsDNA HS Assay Kit, InvitrogenTM Q32851) qPCR and Fragment analyzer. 10 multiplexed libraries were pooled and sequenced in one lane on the Illumina HiSeq4000 sequencing platform (100-bp, paired-end reads)