title: RNA-Seq of hepatocytes
design: Prior to library preparation total RNA samples were evaluated for their integrity using the Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit (Agilent). Libraries for 6 samples were prepared using the Ion AmpliSeq Transcriptome Mouse Gene Expression Kit that covers over 20,000 mouse RefSeq genes in a single assay. Libraries were prepared according to the manufacturer's protocol using 60ng of total RNA as the input material. Six barcoded libraries were combined and pooled in equimolar concentrations and subsequently sequenced on the Ion PI Chip v3 using the Ion Proton Sequencer and the Ion PI Hi-Q Sequencing 200 chemistry. The primary bioinformatic analyses including ltering and alignment of reads were carried out using the Torrent Suite Server.