title: Bioreactor metagenome (Day 456)
design: DNA sample extracted on Day 456 was used for metagenomic sequencing. Libraries were prepared with Nextera DNA Prep Library Preparation Kit (Illumina # 20060059) following the manufacturers guidelines. Library preparation and bead clean-up were further conducted using Mantis Liquid Handler (Formulatrix). A sequencing pool was created by pooling Nextera DNA Prep libraries at equimolar amounts of 2nM/library. The quantification of the library pool was conducted in triplicate with the Qubit dsDNA HS Assay Kit (Invitrogen). Quality control of the library was performed on the Agilent D1000 HS tapes (#5067-5582) using the TapeStation 4200 (Agilent # G2991AA) following the manufacturers instructions. Sequencing was performed on the Illumina NovaSeq6000 using the SP kit v1.5, for 2  150bp paired-end reads, at the Australian Centre for Ecogenomics, Brisbane, Australia.