title: Whole-genome sequence of Staphylococcus epidermidis MRSE 52-2
design: Genomic DNA (gDNA) was extracted from a single bacterial colony cultured in LB broth using the Wizard Genomic DNA Purification Kit (Promega). A 200-bp genomic library was prepared from 1 g of gDNA using the Ion Plus Fragment Library Kit (Life Technologies). Briefly, gDNA was mechanically fragmented to a size range of 50-500 bp (mean 200 bp). The fragmented DNA was then end-repaired, ligated with adapters/barcodes, and size-selected to 330 bp. Subsequently, the genomic library was clonally amplified and enriched. Sequencing was performed using the Ion Torrent PGM platform (Life Technologies) with 200-bp chemistry on a 318 chip.