title: WGS dopamine deficient Drosophila: hi_100
design: DNA isolation from whole flies was performed using an alcohol precipitation protocol. Fragmentation, end repair, dA-tailing, and size selection was then achieved using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina, (NE Biolabs,  Beverly, MA).  Indexing primers for multiplexing were subsequently ligated using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (NE Biolabs,  Beverly, MA