title: Long-read Amplicon Sequencing of lDE11 Plasmid Library
design: Sample plasmid was miniprepped and then the cassette amplified using limited cycle PCR. PCR products were cleaned up with AMPure beads and prepped for library sequencing according to a standard ONT protocol using the ligation sequencing kit (SQK-LSK109). If necessary to achieve adequate depth, multiple sequencing runs were conducted on a single library and the reads pooled.