title: 16S metabarcoding v3-v4 of microbiota of Alouatta seniculus
design: The samples were preserved in a lysis buffer with proteinase K for subsequent DNA extraction in the laboratory. DNA extraction was performed using the NMeasy PowerSoil commercial kit protocol. The extracted DNA was diluted in 50 l of resuspension solution. The quality of the DNA was assessed using Colibr with 2 l of DNA, and DNA integrity was confirmed by visualization on a 1% agarose gel (w/v). Finally, the DNA from each sample was sent for metabarcoding sequencing targeting the v3-v4 region of the 16S rRNA gene.