title: LACE-seq of mus musculus: germinal vesicle stage oocytes
design: The Nanopore sequencing library was prepared with the Ligation Sequencing Kit 1D (Oxford Nanopore Technologies, ONT, SQK-LSK114) following manufacturers instructions. Briefly, cDNA fragments underwent end repair and dA-tailing utilizing the Ultra II End Prep module. Subsequently, the dA-tailed cDNA fragments were ligated to the 1D adapter using the Quick Ligation Module. The resulting libraries were precisely quantified and quality-checked and then load it onto a PromethION flow cell for sequencing on the Oxford Nanopore PromethION platform.