title: Transcriptomics of mus musculus a male liverwGF 1-2)
design: The technical route of RNA-seq mainly includes RNA extraction, nucleic acid detection, library construction, sequencing on the machine, data quality control and data analysis. Firstly, RNA extraction and nucleic acid detection are carried out to obtain high-quality RNA samples. Then, library construction is performed, followed by sequencing on the machine to obtain a large amount of short-read sequence data. Subsequently, data quality control is conducted to remove low-quality sequences and impurities, ensuring the accuracy of the data. Finally, data analysis is carried out, including reference genome alignment, quantification of gene expression levels, identification of differentially expressed genes, functional enrichment analysis, and identification of alternative splicing and new genes. Through these steps, we can comprehensively understand the expression and functional enrichment of inter-group differentially expressed genes, providing a theoretical basis for exploring the molecular mechanisms of biological development, stress response, and the occurrence and development of diseases and tumors.