title: RNA-seq of Arabidopsis thaliana root: wild type
design: RNA was extracted with TRIzol reagent, DNA was digested by DNase I and RNA was double purified by LiCl precipitation. The quality of RNA was checked by the RNA BR Tape and the concentration was determined by the Qubit BR RNA kit. RNA-seq libraries were prepared with 1 g purified RNA using the TruSeq Stranded mRNA kit from Illumina, according to the kit manual.