title: Oryza sativa stem sample:SQ24073575-KO2-KO2_combined_R1
design: ATAC-seq sequencing and analysis were performed on stem tissues of WT and KO rice plants at the jointing stage quality assessment, and library sequencing. After confirming that the sample genomic DNA passed quality inspection, it was fragmented via mechanical shearing (ultrasonication). The fragmented DNA was then subjected to a series of steps including fragment purification, end repair, 94'-end A-tailing, and adapter ligation. Following this, size selection was performed using agarose gel electrophoresis. The selected fragments were subsequently amplified by PCR to generate the final sequencing library. The constructed library underwent rigorous quality control, and only those libraries passing the quality assessment were proceeded to the sequencing stage.