title: IbCHYR1,day30,CHIP
design: leaf tissue (2 g) was cross-linked in 1% formaldehyde under a vacuum. The cross-linking was stopped by the addition of 0.125 M of Gly. The sample was ground to a powder in liquid nitrogen and subjected to nuclear isolation. Anti-IbCHYR1 (1:150 dilutions) antibodies were used to immunoprecipitate the proteinDNA complex, and the precipitated DNA was recovered.