title: KRSE4-3 ROV SED 1
design: The DNA was extracted from each sample (up to 10 g) using DNeasy PowerMax Soil Kit (Qiagen, Hilden, Germany) following the manufacturers protocol. DNA concentration and integrity were checked using Qubit dsDNA HS Assay and Qubit fluorometer 4.0 (ThermoFisher Scientific, Waltham, USA) and agarose gel (1%) electrophoresis, respectively. The microbial DNA extracted was subjected to bacterial and archeal 16S and ITS rRNA,  gene sequencing at MR DNA (Molecular Research LP, Shallowater, USA). The DNA was amplified using the universal primer for fungal studies ITS1 (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3)