title: GBS data of leaf
design: Fifteen P. americana populations were used for genotyping. A genotyping - by - sequencing (GBS) approach was employed, using EcoRI and NlaIII restriction enzymes for digestion. The GBS library was constructed with the Illumina TruSeq DNA Library Preparation Kit following the manufacturer's protocol. Sequencing was performed on the Illumina HiSeq 2500 platform.