title: RNA-Seq of YT103: Weissella kandleri
design: The experiment used the TruSeq Stranded Total RNA Library Prep Kit to construct the library. In the dNTPs reagent for synthesizing the second strand of cDNA, dUTP was used instead of dTTP, making the second strand of cDNA contain A/U/C/G. Before PCR amplification, UNG enzyme was used to digest the second strand of cDNA, so that the library only contains the first strand of cDNA.Sequencing on the MachineQuantified with Qubit 4.0, mixed and loaded onto the machine according to data ratios;Bridge PCR amplification is carried out on the cBot to generate clusters;Sequence on the machine.