title: WGS of Vibrio campbellii PH1401
design: V. campbellii strain PH1401 was cultured on marine agar and subsequently grown in Luria-Bertani broth supplemented with 2.5% NaCl at 30C overnight. Genomic DNA was extracted using the Nanobind HMW DNA extraction kit (PacBio, Menlo Park, USA) and sequenced using both Illumina and Oxford Nanopore Technologies (ONT) platforms. For Illumina sequencing, genomic DNA was sheared to ~350 bp using a Covaris M220 focused ultrasonicator and prepared without PCR amplification using the KAPA LTP library preparation kit (KK8232, Roche). Paired-end sequencing was performed on a NovaSeq 6000 using an S4 reagent kit v1.5 (300 cycles), generating 9,639,510 total reads totaling 1455.4 Mbp.