title: ITS4Seq of Ans FH1
design: The extracted DNA of the screening strain was used as a template for PCR amplification using the universal primers ITS1 (5'-TCCGTAGGTGAACCTGCGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3'), and the amplified PCR products were sent to Tsingke Biotechnology (Beijing, China) for sequencing. Sanger sequencing of PCR amplicons without library construction