title: RNA-Seq of apple calli
design: Transcriptome sequencing was performed on apple callus tissues transformed with the control empty vector and those overexpressing the PpSAMS gene, respectively. Based on the Illumina sequencing platform, Oligo dT magnetic beads were used to enrich mRNA from total RNA. After fragmentation, the first - strand cDNA was synthesized using random hexamer primers, followed by the synthesis of the second - strand cDNA. After end repair, A - tail addition, adapter ligation, fragment selection, amplification and purification, the library was ready.