title: RNA-seq of HepG2 cell line
design: RNA extraction, library preparation, and next-generation sequencingRNA was extracted using the FastPure Complex Tissue/Cell Total RNA Isolation Kit purchased from Vazyme. Eukaryotic RNA isolates were quantified using a fluorometric RiboGreen assay. The total RNA integrity was assessed using capillary electrophoresis. Only samples higher than 1 microgram with a RIN of 8 or greater proceeded to sequencing. Total RNA samples were converted to Illumina sequencing libraries using VAHTS Universal V10 RNA-seq Library Prep Kit for Illumina.