title: Th1 of Homo sapiens, WGBS, control
design: Genomic DNA, spiked with lambda DNA, was fragmented into pieces ranging from 200 to 400 base pairs. These fragments were then treated with bisulfite to produce single-stranded DNA (ssDNA). Next, methylation sequencing adapters were ligated to the ssDNA, followed by synthesis of double-stranded DNA to create the library. The library was then prepared by size selection and PCR amplification. It was quantified using Qubit and real-time PCR, and the size distribution was analyzed using a bioanalyzer. The quantified libraries were pooled and sequenced on Illumina platforms.