title: WGS of Gallus gallus:adult male blood
design: A total amount of 0.2 g DNA per sample was used as input material for the DNA library preparations. Sequencing library was generated using NEBNext UltraTM DNA Library Prep Kit for Illumina (NEB, USA _x0001_Catalog #: E7370L) following manufacturers recommendations and index codes were added to each sample. Briefly, genomic DNA sample was fragmented by sonication to a size of 350 bp. Then DNA fragments were endpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing, followed by further PCR amplification. After PCR products were purified by AMPure XP system (Beverly, USA). Subsequently, library quality was assessed on the Agilent 5400 systemAgilent, USA and quantified by QPCR (1.21 nM).