title: WGS of Vibrio campbellii PH1401
design: For Oxford Nanopore Technologies (ONT) sequencing, DNA was purified using AMPure XP reagent (Beckman Coulter) and enriched for high molecular weight fragments using the SRE XL kit (PacBio, Menlo Park, USA). Libraries were prepared using the ligation sequencing kit (SQK-LSK109) with native barcoding expansion 96 (EXP-NBD196) and sequenced on a MinION with an R9.4.1 flow cell (FLO-MIN106D). Basecalling was performed with Guppy v6.4.2 using the high-accuracy model, producing 11,333 reads totaling 295 Mbp.