title: Sequencing and analyzing the whole genome of HJ01
design: The SMRT Bell library was constructed using the SMRT Bell TM Template kit (version 2.0). DNA samples that passed electrophoresis detection were fragmented into the desired fragment sizes required for library construction using the Covaris g-TUBE. The DNA fragments underwent DNA damage repair and end repair. Hairpin adapters were ligated to both ends of the DNA fragments using DNA ligase, followed by purification of the DNA fragments using AMpure PB magnetic beads. Specific-sized fragments were selected using Blue Pippin. The SMRT Bell library was subjected to concentration selection using AMpure PB magnetic beads, followed by DNA damage repair. The SMRT Bell library was purified again using AMpure PB magnetic beads. The constructed library was quantified for concentration using Qubit and analyzed for insert size using the Agilent 2100. Finally, sequencing was performed using the PacBio platform.