title: Pustulosa necki RNA pool-seq
design: All RNA was extracted using the RNeasy kit following the manufacturers protocol (Qiagen). RNA quality and integrity were determined using a NanoDropTM and an Agilent Bioanalyzer (Santa Clara, CA, USA), respectively. Messenger RNA was isolated from 150 ng of total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer; Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit, miniaturized to 2/5 reaction volume and automated on a Sciclone NGSx liquid handler. All libraries were sequenced by the Texas A&M AgriLife Genomics and Bioinformatics Service on an Illumina NovaSeq S4 XP using 2 X 150 bp reads.