title: rat 210, tMCAO, left (contralateral) hemisphere
design: tMCAO in experimental rats was initiated by endovascular occlusion of right middle cerebral artery using monofilament for 90 minutes followed by reperfusion. The anaesthesia was induced by 3% isoflurane and maintained by 1.5-2% isoflurane. Anesthesia was used only for the filament insertion and removal, while in between these procedures the rats were taken out of anesthesia. At 24 h after tMCAO operation, rats were decapitated. The subcortical structures of the brain were isolated from the left (contralateral) hemisphere. The RNAse R-treated fraction from total RNA (circular RNA-fraction) was used for RNA-seq. Sequencing was carried out using an Illumina HiSeq 2500 instrument. At least 20 million reads (1/200 nt) were generated.