title: 16S RNA of human gut metagenome
design: The target fragments of the 16S rRNA gene were amplified with standard primers for the V3V4 region. The purified PCR products were indexed with the KAPA HiFi HotStart ReadyMix (2X) (Roche Diagnostics, Switzerland) and Nextera XT Index Kit (Illumina, USA). The obtained libraries were sequenced using the Illumina MiSeq platform.