title: D1-D2 gene amplicon of bovine: rumen liquid
design: Rumen samples were collected by using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted using Qiagen Dneasy Power Soil Pro kit. For rumen fungi sequencing primers F: GCGTTTRRCACCASTGTTGTT and R: GTCAACATCCTAAGYGTAGGTA were used.