title: CTX-M-15 producing Klebsiella pneumoniae ST709 blood isolate
design: Sequencing libraries were prepared using KAPA HyperPlus Kit (Roche,124 Grenzacherstrasse, Basel, Switzerland).Briefly, 10ng of genomic DNA was used as input material for library preparation, where, enzymatic fragmentation of genomic DNA is performed, followed by end repair to generate blunt end fragments. The DNA fragments were further subjected to adenylation followed by adapter ligation and amplification using limited cycle PCR, during which, dual indices were added to the end of the DNA fragments. The amplified product was subsequently purified using a bead based clean up step generating libraries for use on an Illumina sequencing system. The quality of libraries were checked using high sensitivity D1000 Screen Tape in Agilent 2200 TapeStation system and quantified using dsDNA High Sensitivity kit in Qubit system (Thermo Scientific). Paired end (2x250bp) sequencing of these libraries were performed in Novaseq 6000 (Illumina).