title: RNA-Seq of Solanum galapagense & lycopersicum: 8 week old plants at 5 leaf stage harvested distal roots
design: After 21 days of treatment, the bottom 5-cm from the root tips (three biological replicates) were excised and triple washed with DI water, stored in RNAse free 50-ml Falcon tubes (USA Scientific, Ocala, FL, USA), frozen in liquid N within ~10 min, and stored at -80 C until processing. Total RNA was extracted using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, Saint Louis, MO, USA), in-column DNAse treated, purified and concentrated using the RNA Clean and ConcentratorTM (Zymo Research, Irvine, CA, USA). All samples had an A260/280 1.8 and an RNA Integrity Number 8.