title: 18S rRNA amplicon from oil exposure experiment
design: 100 mL sampels were collected at each time point and filtered through a 0.22 um filter. The filter was flash frozen in liquid nitrogen and stored at -80C. The DNA was extracted using a modified CTAB method and the V4-5 region of the 16S rRNA gene and the V4 region of the 18S rRNA gene were amplified with fusion primers. Cleaned products were pooled and sequenced using 300 bp paired-end methods on an Illumina MiSeq.