title: Shotgun:Marine sediment:Tasmania
design: Frozen sediment (0.25g), Combined extraction (Armbrecht et al., MER, 2020), 20uL library input, blunt-end repair, adapter ligation, Bst fill-in reaction, 1st amplificiation (IS7/IS8) with 22 cycles, 2nd amplification (IS4/GaII-Index) with 13 cycles, cleanup with beads, bead-based size-selection (<~500bp), Tapestation quantification