title: RNA-Seq of Homo sapiens: renal cell carcinoma tumor without venous invasion
design: Bulk RNA sequencing of renal cell carcinoma specimens from the Rodriguez cohort. Tissue types include primary tumor, tumor thrombus extending into the venous system (RITE), and normal adjacent kidney tissue. Total RNA was extracted using TRIzol reagent. Ribosomal RNA was depleted and libraries were prepared using the KAPA Stranded RNA-Seq Kit with RiboErase. Libraries were sequenced on an Illumina NextSeq 500 platform producing 75 bp paired-end reads.