title: DNA metabarcoding library of 18S: bot layer of nest
design: The libraries were prepared using a two-step PCR method: the first PCR using universal 18S primers: SSU F04 [5-GCTTGTCTCAAAGATTAAGCC-3]SSU R22 [5-GCCTGCTGCCTTCCTTGGA-3]  (Blaxter et al. 1998) with illumina overgangs (PCR conditions: 95"C x 5' 35x [95C x 30" + 51C x 1'30" + 72C x 1'] 72C x 10), and the seccond PCR with Nextera XT Index Kit (Illumina, 2013). Pooled purified libraries were sequenced on an AVITI (Element Bioscience) (2  300 cycles).