title: 18S rRNA gene amplicon of bovine: rumen liquid
design: Rumen samples were collected by using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted using Qiagen Dneasy Power Soil Pro kit. For ciliate protozoa amplicon sequencing primers P-SSU-316F/GIC758R were used.