title: 25mM Lactose (pre-growth Glucose)
design: Illumina libraries were prepared using a one-step PCR with gDNA as template. 2 L of primers and 10 L of gDNA were used for each 50 L PCR reaction with 25 L 2x Phanta Master Mix (#P515-02  Vanzyme). After a denaturation step at 95C for 1 min, a 30x loop consisting of 15 sec at 95C, 15 sec at 55C (annealing), and 1 min at 72C (elongation), followed by 10 min at 72C was conducted. The PCR amplicons (303 bp) were purified from a 2% agarose gel using Macherey-Nagel NucleoSpin Gel and PCR Clean-up Kit (#REF 740609.50 - Macherey-Nagel). The amount of DNA was quantified by Qubit assay (Q33231 - Invitrogen).