title: cultivable_Cistanche deserticola_Orobanchaceae_site 3_IC10
design: DNA was extracted from various disinfected plant organs using the innuPREP Plant DNA kit (Analytik Jena AG, Berlin, Germany) protocol. For endophytic cultivated bacteria, genomic DNA was extracted using the DNeasy PowerSoil kit (Qiagen). 16S primers targeting the V3 and V4 regions of the 16S rRNA gene were used, resulting in the construction of three libraries: the root endophytic plant microbiome library, the green part endophytic plant microbiome library, and the culturable endophytic bacteria library.