title: Amplicon Sequencing of Mother's milk
design: Human milk microbiome 16S rRNA amplicon survey of early and late lactation samples collected in Guatemala. Milk was collected by full manual expression by a trained midwife after nipple/areola cleaning with 70% ethanol, stored on ice, aliquoted at the field laboratory, transferred on dry ice, and stored at -80C. DNA was extracted from 1 mL milk using the Qiagen DNeasy Blood and Tissue kit. The V1-V3 region of the bacterial 16S rRNA gene was amplified with primers 27F and 533R and sequenced on the Illumina MiSeq platform.