title: CRE long-read WGS
design: Genomic DNA extracted from pure bacterial cultures was quantified using a NanoDrop 2000 spectrophotometer, and DNA purity and integrity were assessed prior to library preparation. Approximately 1 ug of genomic DNA per isolate was used as input material. Sequencing libraries were prepared using the Rapid Barcoding Kit (SQK-RBK114.24) according to the manufacturers instructions. Barcoded samples were pooled and sequenced on a MinION Mk1C device using R10.4.1 flow cells.