title: RNA-seq of Myotis lucifugus: JV6-SW, 6 hours, control, Rep3
design: 500,000 cells were treated in cell culture media (DMEM/F12, 10% FBS, 1% pen-strep) supplemented with either 100uM neocarzinostatin (Sigma-Aldrich N9162) or with 4.4mM vehicle (MES buffer, Sigma-Aldrich M1317) for 6 hours in triplicate on a 6-well dish. Cells were gently washed twice with DPBS before lysing in-place with ice-cold Buffer RLT Plus (Qiagen 1053393) supplemented with 40mM DTT. After cell lysis, RNA was extracted following the RNEasy Plus Mini Kit protocol (Qiagen 74134). Poly-A selection,  library preparation, and sequencing was performed by Novogene (Davis, CA, USA).