title: pETDuet-dsn3PLS-DYW + pCOLA_His6-RIP2
design: Total RNA was extracted from 1 mL of E. coli bacterial pellet as for the detection of RNA editing. RNA concentration and integrity were assessed using a Qubit 2.0 Fluorometer (Invitrogen) with the Qubit RNA BR Assay Kit (Invitrogen Ref No. Q10211) and an Agilent 2100 Bioanalyzer (Agilent Technologies) with the RNA 6000 Nano Kit (Agilent, Cat No. 5067-1511). RNA libraries were treated with the FastSelect rRNA depletion kit from Qiagen for bacterial depletion and a directional library preparation was used at Cornell RNA Core facility. The libraries were sequenced on an Illumina NovaSeq X instrument with unpaired-end 150 bp reads.