title: 16S of human: Drug-Sensitive Subject 18
design: A stool sample was collected from each participant at the time of the enrollment by spontaneous or enema defecation using a self-collection stool kit (CeGat GmbH, Tbingen, Baden-Wrttemberg, Germany). The sampling kit contained a liquid-based DNA preservation solution and samples were stored at room temperature (20-25 C) until DNA extraction. Total DNA was extracted using the Zymo BiomicsDNA/Rna Miniprep Kit (Zymo Research) and quality was checked with Qubit dsDNA BR or HS (Thermo Fisher), as per manufacturers protocol. Twenty ng of DNA were used for library preparation by amplifying the V3-V4 hypervariable regions of the bacterial 16S rRNA gene using the Zymo Quick 16S NGS library preparation kit (Zymo Research). The final libraries were pooled and sequenced with MiSeq Sequencing System (Illumina) for 2x250 base paired-end reads.