title: single RNA-seq for mus musculus:colon,DSS-induced colitis
design: We isolated colonic cells from wild-type and VISTA-KO mice treated with dextran sulfate sodium. The CD45+ immune cells were further isolated through flowcytometry sorting procedure. The single cell RNA sequencing was then applied to analyse the role of VISTA upon immune response in colitic mice. Sample tags were used for wild-type or VISTA-KO annotation since all immune cells were pooled into one sample for sequencing process.