title: 16S rRNA gene amplicon sequencing of seawater bacterial communities from an experimental aquatic system
design: Seawater samples were collected from an experimental aquatic system containing sterilized or non-sterilized bioballs with shrimp feed supplementation. Two experimental groups were established, each with four biological replicates. Samples were collected over a 25-day experimental period on days 1, 4, 11, and 18. Bacterial DNA was extracted from seawater samples and subjected to 16S rRNA gene amplicon sequencing targeting the V3V4 regi