title: 16S rRNA seq of mouse: feces IKO
design: DNA was extracted from mouse feces using the CTAB/SDS method. 16S rRNA genes in distinct regions (V3V4) were amplified using Phusion High-Fidelity PCR Master Mix (NEB). Following manufacturers recommendations, sequencing libraries were generated with NEBNext Ultra IIDNA Library Prep Kit (Cat No. E7645).