title: Black soybean oil treated mice
design: Project enriches mRNA with Oligo(dT) beads, fragments to ~300 bp. Synthesizes cDNA, builds 450 - bp library. Enriches by PCR, checks quality, measures concentration. Mixes indexed libraries, dilutes to 2 nM, denatures. After RNA prep and library build, does paired - end NGS on Illumina.