title: Illumina WGS of a Candida dubliniensis isolate
design: DNA samples (100 to 200 ng) were sheared using a Covaris S2 Focused-ultrasonicator and libraries were prepared using the New England Biolabs NEBNext Ultra II DNA Library Prep kit (cat#E7634L) with an average insert size of 450 bp.  PCR-amplified libraries were qualified on an Agilent Technologies 4150 TapeStation using a D1000 ScreenTape assay (cat# 5067-5582 and 5067-5583) and the molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (cat#KK4824).  Libraries were normalized and pooled in preparation for Illumina sequencing