title: Colletotrichum fructicola
design: For RNA-seq analysis, mycelia were harvested by filtration from PDB mixed cultures after inoculation with conidia at 25C for 48 h. To explore gene expression during leaf infection, fully expanded leaves with uniform size were sprayed until runoff with a conidial suspension. Each strain was used to inoculate four leaves, which were then incubated in moist chambers at 25C. At 72 h post inoculation (hpi), four leaves inoculated with the same strain were mixed as one sample.