title: Leucobacter sp. WKLB1 genome
design: To isolate the microbes, we first crushed the pegmatites and suspended them in phosphate-buffered saline (PBS). This suspension was then applied to LB agar medium and incubated at 20C.  We obtained pure colonies by repeatedly picking single colonies from the plates. A single colony was then selected and incubated in LB liquid medium for 64 hours. After incubation, the cells were harvested by centrifugation (5000 G, 5 min., 4C), and the medium was removed.  Genomic DNA was extracted from the lysate using liquid nitrogen and the NucleoBond HMW DNA kit (Macherey-Nagel) by following the manufacturer's protocol with an extended incubation time of one hour. Long-read sequencing libraries were prepared using the Rapid Barcoding Kit (SQK-RBK114.24, Oxford Nanopore Technologies) and sequenced on the MinION Mk1B device using MinKNOW v.24.11.8 (Oxford Nanopore Technologies).  Sequenced reads were basecalled, demultiplexed, and trimmed for adapter sequences using Dorado v.0.9.0 (super accurate mode).