title: NGS Chicken Bursa of Fabricius
design: Total RNA was extracted using a TRIzol-based RNA extraction kit (GeneAll Hybrid-R, South Korea) in accordance with the manufacturers instructions. The extracted RNA was subjected to complementary DNA (cDNA) synthesis using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, USA). The RNA was used as a template to amplify the complete genomic segments of infectious bursal disease virus (IBDV), while Newcastle disease virus (NDV) genomic fragments were amplified from the synthesized cDNA using overlapping amplicon primers.