title: 16S rRNA gene amplicon sequencing of metagenome: germ-free Bama pig cecum, 21 days
design: The V3V4 region of the 16S rRNA gene was amplified using primers ACTCCTACGGGAGGCAGCA and GGACTACHVGGGTWTCTAAT. Amplicon libraries were generated by PCR and sequenced using a paired-end strategy. Sequence data were processed using DADA2 (v1.1) with taxonomic assignment based on the gg2_2022_10 reference database.