title: 16S rRNA high-throughput amplicon sequencing of seawater samples (Caniogan Reef)
design: Bacterial 16S rRNA V3-V4 hypervariableregion was amplified using Herculase II Fusion DNA Polymerase Nextera XT IndexKit V2 kit (Agilent Technologies, Santa Clara, CA, USA) with primers Bakt_341F (5-CCTACGGGNGGCWGCAG-3) and Bakt_805R (5-GACTACHVGGGTATCTAA-TCC-3)