title: NIOT-MBT
design: Genomic DNA was extracted from an axenic bacterial isolate and used for library preparation following the Illumina tagmentation-based library preparation protocol. Briefly, genomic DNA was subjected to enzymatic fragmentation and adapter tagging (tagmentation), followed by post-tagmentation cleanup. Tagmented DNA was PCR-amplified to generate sequencing-ready libraries, which were subsequently purified. Library size distribution and quality were assessed using an Agilent TapeStation system. DNA concentration at all steps was measured using the Qubit Fluorometer with appropriate controls. Final libraries were pooled and sequenced (2  150 bp) on the Illumina NextSeq 2000 platform. High-quality sequencing data (>Q30) were used for downstream analysis.