title: 18S rRNA - Based Sequencing
design: 30ng qualified DNA template and the 16S rRNA fusion primers are                 added forPolymerase chain reaction (PCR). All PCR products are purified by                 AgencourtAMPure XP beads, dissolved in Elution Buffer and eventually labeled to                 finish libraryconstruction. Library size and concentration are detected by Agilent                 2100Bioanalyzer. Qualified libraries are sequenced according to their insert                 size.