title: pETDuet-dsn3PLS-DYW_genomic DNA library
design: Genomic DNA (gDNA) was extracted from 1 mL of E. coli bacterial pellet for genomic analysis. DNA concentration and integrity were assessed using a Qubit 2.0 Fluorometer (Invitrogen) with the Qubit dsDNA BR Assay Kit (Invitrogen Ref No. Q32850) and an Agilent 2100 Bioanalyzer (Agilent Technologies) with the DNA 1000 Kit (Agilent, Cat No. 5067-1504). The gDNA was randomly fragmented, and a library was prepared using standard Illumina library preparation protocols. The libraries were sequenced on an Illumina NovaSeq 6000 instrument with paired-end 150 bp reads.