title: 16S Amplicon sequencing from sand dunes of Sonoran desert
design: DNA was extracted using the DNeasy PowerSoil Pro kit (Qiagen). The V3V4 region of the 16S rRNA gene was amplified using primers 341F and 805R. Libraries were prepared with Nextera XT and sequenced on an Illumina MiSeq platform (300 bp paired-end).