title: Supplementation of curcumin promotes the intestinal structure, immune barrier function and cecal microbiota composition of laying hens in early laying period
design: After library quality control, different libraries were pooled based on the effective concentration and targeted data amount. 5'-end of each library was phosphorylated and cyclized.Subsequently, loop amplification was performed to generate DNA nanoballs. These DNAnanoballs were finally loaded into flowcell with DNBSEQ-T7 for sequencing in NovogeneBioinformatics Technology Co., Ltd (Beijing, China).