title: RNA-Seq of  grape roots
design: Transcriptome sequencing was performed on grape roots with and without arbuscular mycorrhizal treatment, respectively. Based on the Illumina sequencing platform, Oligo dT magnetic beads were used to enrich mRNA from total RNA. After fragmentation, random hexamer primers were used to synthesize the first - strand cDNA, followed by the synthesis of the second - strand cDNA. After end repair, A - tail addition, adapter ligation, fragment selection, amplification, and purification, the library was ready.