title: circRNA sequencing of Y79 cells
design: Total RNA was extracted using HiPure Total RNA Mini Kit (Magen, China), with integrity verified via Agilent 2100 Bioanalyzer (RIN >7.0) and quantified by Qubit 3.0 Fluorometer (InvitrogenUSA). rRNA depletion was performed using RNase R (EpicentreUSA), followed by directional cDNA synthesis with NEBNext Ultra Directional RNA Library Prep Kit. Indexed adapters were ligated using KAPA RNA HyperPrep Kit (Kapa BiosystemsUSA), and libraries were amplified by PCR, quantified (Qubit), and pooled equimolarly