title: metagenome
design: The sediment samples (0.5 g) were extracted for DNA sequencing. DNA was extracted using the CTAB method, and the concentration of the extracted nucleic acids was determined using a Qubit 3.0 fluorometer (Invitrogen, reagent Qubit dsDNA HS Assay Kit). The qualified DNA was then subjected to a series of processes, including digestion, end repair, library amplification, and product purification, collectively forming a sequencing library. Each metagenomic library generated approximately 10 Gb of raw sequencing data. After successfully completing the library quality control process, each sample was subjected to macrogenome sequencing using 150-base paired-end sequencing on an Illumina NovaSeq 6000 platform.