title: RNA-seq of Ectopic endometriotic lesion isolated from an experimental mouse model of endometriosis
design: Total RNA was extracted from biological samples and subjected to quality control according to sample and product requirements. Polyadenylated mRNA was enriched from total RNA using oligo(dT) magnetic beads after heat denaturation to disrupt RNA secondary structures. The purified mRNA was then fragmented under controlled temperature and time conditions to generate appropriately sized RNA fragments.