title: PromethION sequencing
design: HG002 (Coriell Institute, GM24385) was cultured in RPMI1640 (Gibco, 11875093) with 15% fetal bovine serum (Bovogen, SFBS-AU) at 37Â°C with 5% CO2. DNA was extracted using Nanobind CBB kit (PacBio, 102-301-900). DNA was sheared to ~65 kb using the Megaruptor 3 (Diagenode) and libraries prepared using high duplex Ligation Sequencing Kit V14 (SQK-LSK114, Oxford Nanopore Technologies) protocol and sequenced on a R10 Version HD Flow Cell (FLO-PRO114HD, Oxford Nanopore Technologies) at 5kHz sampling rate.