title: ISO-Seq of Chorispora bungeana:c3
design: Total RNA are synthesized to first-strand cDNA using Clontech SMARTer PCR cDNA Synthesis Kit. After PCR Optimization, a large-Scale PCR is performed to synthesize second strand cDNA. After another large-Scale PCR, the DNA is ready for SMRTbell Template Preparation and sequencing(we pooled a BluePippin size selection library to no size selection library before sequencing, The arranges of size selection are >4kb).