title: Oxford Nanopore shotgun metagenomic sequencing of KJA hot spring biofilm
design: Shotgun metagenomic sequencing was performed on DNA extracted from a thermophilic hot spring biofilm using the Oxford Nanopore Technologies PromethION platform. Sequencing was conducted using an R10.4.1 flow cell with e8.2 chemistry at a translocation speed of 400 bp/s, and basecalling was performed using Guppy v4.3.0 in high-accuracy mode.