title: Control_2 granulosa cell RNA-seq library
design: Granulosa cells were collected from female Mus musculus in three groups: Control, cyclophosphamide plus PBS (Cy+PBS), and cyclophosphamide plus embryonic stem cell-derived extracellular vesicles (Cy+EVs). Four mice were included in each group, and two biological replicates per group were prepared by pooling granulosa cells from two mice per replicate. Total RNA was extracted using TRIzol reagent (Invitrogen), and RNA quality was assessed using NanoDrop and an Agilent Bioanalyzer 2100; samples with RIN > 7.0 were used for library construction. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, followed by cDNA library preparation according to standard protocols. Libraries were sequenced on an Illumina NovaSeq 6000 platform using paired-end 150 bp reads.