title: ONT-seq of Eleutheronema tetradactylum
design: For ONT sequencing, we constructed an ultra-long library and then one flow cell was sequenced on a PromethION platform (Oxford Nanopore Technologies Co., UK). The raw reads were first refined to remove those with quality value (QV) below 7. Subsequently, Porechop (https://github. com/rrwick/Porechop) was applied to eliminate adaptors, and Filtlong (https://github.com/ rrwick/Filtlong) was employed to filter out those reads shorter than 30kb and mean read quality scores less than 90%.