title: Transcriptomics of HEK293T cells expressing AFF4-wild type or AFF4-T829/S831/S833/S834/S835A mutant treated with or without insulin
design: RNA was prepared using the Qiagen RNeasy Mini Kit (Qiagen) according to the manufacturers protocol. Total RNA quantity and quality were assessed using the high-sensitivity R6K ScreenTape system (Agilent# 5067-5582). An input of 500 ng total RNA for each sample was indexed separately using the TruSeq Prep RNA Kit v2 (Illumina) according to the manufacturers instructions. Each library was quantified using the Agilent Tapestation (D1000 Screen Tape Agilent# 5067-5582). The indexed libraries were normalized to 1000 pM and subjected to cluster and paired-end sequencing (2x 166 cycles) was performed using P2 300 cycle High Output Kit (Illumina) on a NextSeq 2000 Illumina instrument, yielding a minimum of ~40 million total reads per sample.