title: RNA-Seq of mus musculus: male liver
design: cDNA libraries were constructed via : mRNA enrichment with Oligo(dT) magnetic beads  fragmentation (200300 bp)  double-stranded cDNA synthesis  300 bp cDNA fragment selection (including end repair, A-tail addition, and adapter ligation)  Illumina paired-end sequencing. Raw sequencing data were filtered using fastp software by removing low-quality reads, adapter contamination, and unknown bases (N), while retaining reads with a length of 35 bp. The filtered clean data were then aligned to the mouse reference genome using STAR software, with gene expression levels normalized by fragments per kilobase of transcript per million mapped reads (FPKM) and evaluated via RNA-SeQC (alignment rate >85%).