title: RNA-Seq of homo sapiens: nasal sample
design: Nasal and blood samples were collected from adults with newly confirmed HIV and TB symptoms. Total cellular RNA from human nasal and PAXgene blood samples was extracted using the RNeasy Plus Micro Kit protocol. To generate the cDNA library, the NEBNext rRNA Depletion Kit was used to remove the ribosomal RNA. Illumina-compatible RNA-seq library was then prepared using the NEB Next Ultra II RNASeq library preparation kit. The cDNA libraries were purified with AmpureXP beads and quantified using an Agilent TapeStation and a Qubit 4 Fluorometer.  Libraries were sequenced on the Illumina NovaSeq S2 FC 6000 to generate paired-end RNA-seq data. The goal of the study was to identify nasal and blood gene expression differences between adults with microbiologically confirmed active TB disease and adults without TB disease.