title: RNA-seq of PMA-activated human monocytes THP-1
design: Directional cDNA libraries were created by means of the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina. Size selection of DNA fragments was performed on Agencourt AMPure XP beads (Beckman Coulter, USA). Next, PCR enrichment of the adapter-ligated library was conducted (six cycles of PCR)