title: HAEC_atac_oxldl3_batch2
design: HAECs were cultured and harvested at passage 4-5. ATAC-seq libraries were prepared according to the Omni-ATAC protocol. The quality of DNA samples was analysed using a TapeStation 2200 with High Sensitivity D1000 ScreenTape (Agilent), with successful transposition indicated by fragments piling up with a nucleosome-free peak at around 25 bp and larger peaks between 160~200 bp apart. Indexed libraries were quantified by qPCR using a KAPA Library Quantification kit (Roche) and pooled in equimolar amounts for sequencing with the Oxford Genomics Centre. Libraries were sequenced on a NovaSeq 6000 device (Illumina with the target depth of 112.5 M pairs of 150 bp reads per sample.