title: RNA-Seq of toxoplasma
design: After the library QC, it is pooled according to the effective concentrationand the target data output required for the experiment, then subjectedtoIllumina sequencing, which produces 150 bp paired-end reads. Thebasicprinciple of the sequencing is "Sequencing by Synthesis," where fluorescently labeled dNTPs, DNA polymerase, and adapter primersareadded to the sequencing flow cell for amplification. As each sequencingcluster extends its complementary strand, the addition of each fluorescently labeled dNTP releases a corresponding fluorescencesignal. The sequencer captures these fluorescence signals and converts theminto sequencing peaks through computer software, thereby obtainingthesequence information of the target fragment.