title: RNA-seq of Bombyx mori: Sxl- late-pupal soma
design: Tissues were lysed with Trizol and total RNA was isolated using Direct-zol RNA Miniprep Plus Kit (Zymo Research). rRNA depleted libraries were generated using Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research) and indexed with Zymo-Seq Unique Dual Index (UDI) Primer Plate (Zymo Research) according to the manufacturers instructions. Libraries were sequenced on a NovaSeq 6000 SP100 flow cell (50 bp paired-end sequencing, Illumina).