title: MiSeq_sequencing_of_PylRS_sequences_after_PANCE
design: Samples were prepared according to the AmpliSeq protocol by Cruaud et al., 2017. A total of eight primer pairs were used for gene amplification, designed to cover the entire gene length twice. Samples were purified from adapters and short fragments using Trimmomatic v0.39. Sequencing was then performed on an Illumina MiSeq.