title: DNA-Seq of zebrafish: ELS stool rep 1-5
design: DNA was extracted from zebrafish stool using the QIAmp Fast DNA Stool Mini Kit. 12.5ng of total DNA were amplified using universal primers targeting the V4 region of the bacterial 16S rRNA gene. Each 16S amplicon was purified using the AMPure XP reagent (Beckman Coulter). Each sample was amplified using a limited cycle PCR program, adding Illumina sequencing adapters and dual-index barcodes to the amplicon target. The final libraries were purified using the AMPure XY reagent, quantified, and normalized before pooling.