title: Chloroplast Genome sequencing of Saxifraga josephii"
design: DNA library construction and sequencing were performed a pair-end (PE) library with 350 bp insert size fragments using TruSeq DNA sample preparation kits (Sangon, Shanghai, China). Therefore, we sequenced at least 1.2 GB of clean data for S. josephii. The detailed next-generation sequencing was conducted on the Illumina Hiseq 2500 platform by Sangon Biotech (Shanghai, China). Then, we trimmed the low-quality reads by NGSQCToolkit v2.3.3 (Patel and Jain 2012). After removing the low-quality sequences, the clean reads were assembled using MIRA v4.0.2 (Chevreux et al. 2004) and MITObim v1.8 (Hahn et al. 2013, Guan et al. 2023) with the cp genome of S. josephii (PP849681), as reference. A total of 208,396 bp reads generated with average coverage of 266.7. Then, we used DOGMA (http://dogma.ccbb.utexas.edu/) (Mehmood et al. 2020) and Geneious v8.0.2 to annotate the chloroplast genome (Katoh et al. 2013).