title: Illumina Miseq of bacterial (16S) epiphyte/endophyte populations in Honeycrisp stem/calyx end tissue
design: The V4 region of the bacterial 16S region was amplified using the16S primers 515FB (5'-GTGYCAGCMGCCGCGGTAA-3) and 806RB (5'-GGACTACNVGGGTWTCTAAT-3) (Walters et al 2015). In order to prevent the amplification of mitochondrial and ribosomal sequences, these primers were used in conjunction with Peptide Nucleic Acids (PNAs) (PNA Bio) (Lundberg et al 2013). Following DNA purification, reference primers 515FB and 806RB were used to amplify the bacterial 16S region for Illumina MiSeq.