title: Phase II-d
design: For the Bacteria domain, the V3V4 regions of the 16S rRNA gene (~444 bp) were amplified using the universal primers 341FB (CCTACGGGNGGCWGCAG) and 785RB (GACTACHVGGGTATCTAATCC) [25]. For the Archaea domain, the V5V6 regions of the 16S rRNA gene (~450 bp) were amplified using primers Ar915aF (AGGAATTGGCGGGGGAGCAC) and Ar1386R (GCGGTGTGTGCAAGGAGC)