title: eDNA amplicon metabarcoding: marine environmental samples
design: This project sampled environmental DNA from seawater samples collected by CTD rosette in St. Anns Bank Marine Protected in Nova Scotia, Canada. Water samples were filtered through a 0.22um filter in 2021 and 2022, and through a 1.2um filter from 2023 onward. Genetic markers include the 12S MiFishU marker and 16S to target fish, and COI to target general biodiversity using the Leray or modified LerayXT primers. The raw fastq files per marker per year are provided here.