title: RNA-Seq of mIDUA-W392X mouse brain: Scramble gRNA
design: RNA was extracted from mouse brain using the Qiagen RNeasy Plus Universal Mini kit and reverse transcribed  using oligo d(T)23VN and Thermo SuperScript IV.  Target sequences were PCR amplified using IDUA-specific primers possessing Illumina adapter overhangs and KAPA HiFi HotStart DNA polymerase.