title: HybridisationCapture:Marine sediment:Tasmania
design: Input: >50ng of DNA in 7 L (from associated post-IS7/IS8 PCR product); Hybridisation-capture with HABBaits1 (Armbrecht et al., SciRep, 2021); hybridisation temperature: 65 C for 3 hours and then 60 C for 37 hours; bead preparation: 2x washing with binding buffer, then addition of binding buffer and 48 L yeast tRNA (= 480 g per 240 mL beads) in a 3rd washing step; vortexing and incubation on a rotary mixer (30 min, room temperature), pelleting on a magnetic rack, and two more washes with binding buffer.