title: ISO-seq of Spirodela polyrhiza clone 9509 along with Lemna minor clone 8623
design: Total RNA was extracted from 100 mg of plant material was flash-frozen upon harvesting and ground. The samples were homogenized in 1 mL of TRIzolTM (ThermoFisher, #15596018) using Silamat S6 (Ivoclar Vivadent). RNA was extracted according to manufacturers instructions. Iso-Seq libraries were constructed using SMRTbell prep kit 3.0 from RNA