title: STRIPE-seq of CKY5369 C-2
design: Whole RNA extracted from mid log S. cerevisiae cells were DNase(I)d followed by rRNA depletion. Libraries were then prepared following STRIPE-seq protocol by Policastro et al, 2020 with our modifications: 9-base UMI was used; 10-mer RTO was used; dual indexing logos were added in the final step of library amplification. Libraries were purified by 3% polyacrylamide (TBE) gel electrophoresis and sequenced by Illumina NextSeq 2000.