title: RNA-seq in subcutaneous tumor tissue of RM1 from male  Plau-/- mice
design: Total RNA was extracted from the tumor tissues of WT and uPA/ mice, and all RNA-seq samples were quality-controlled quality control, library preparation, and analysis by Genedenovo Biotechnology Co., Ltd. (Guangzhou, China). R version 4.4.0 was used for statistical analysis. A differential gene expression (DEGs) analysis was performed using the DESeq2 R package, with genes with a P-value  0.05 and a log2 fold change of +1 or 1 considered significantly upregulated and downregulated, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the upregulated and downregulated genes were conducted using the ClusterProfiler R package; pathways with an FDR  0.05 were considered significant.