title: DNA metagenome sequencing of stony corals and seawater
design: NEBNext UltraTM II DNA Library Prep Kit for Illumina was used for library preparation, following the manufacturers recommendations. Briefly, the genomic DNA was fragmented by acoustic shearing with a Covaris S220 instrument. Fragmented DNA was cleaned and end-repaired. Adapters were ligated after adenylation of the 3ends, followed by enrichment by limited cycle PCR. DNA libraries were validated using a High Sensitivity D1000 ScreenTape on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA). The DNA libraries were also quantified by real-time PCR (Applied Biosystems, Carlsbad, CA, USA). The sequencing libraries were clustered onto a 25B flowcell on the Illumina NovaSeq X Plus instrument according to the manufacturers instructions. The samples were sequenced using a 2x150 paired-end (PE) configuration.