title: RNA-seq of Homo sapiens: 7658_XX
design: ~10,000 PBMCs per sample were processed using the 10X Genomics Chromium Controller with the Next GEM Single Cell 3' v3.1 Reagent Kit, according to manufacturer's directions. After first strand synthesis was complete, cDNA was pooled for bulk processing as a single sample. The sample was fragmented, end-repaired, A-tailed and ligated with universal adapters. A second sample barcode was added during the PCR step, allowing for unique library identification. Samples were sequenced using a NovaSeq 6000 S4 flow cell with a read length of 150x150 base pairs. Quality-control analysis was completed using the ThermoFisher Scientific Qubit Fluorometric Quantification 3 and the Agilent 2100 Bioanalyzer.