title: hTERT RPE-1 WT cells, untreated, replicate 3
design: RNA-Seq libraries were prepared with KAPA Stranded mRNA-Seq Illumina Platforms Kit (Roche) following the manufacturer's recommendations. Briefly, 500 ng of total RNA underwent poly-A enrichment, fragmentation, and second-strand synthesis with dUTP. The resulting cDNA was adenylated, ligated with adaptors (Integrated DNA Technologies), and enriched with 15 PCR cycles.