title: RNA-seq of rosa hybrida: flower petals
design: Enrich eukaryotic mRNA using Oligo(dT)-coated magnetic beads. Fragment mRNA with fragmentation buffer, then synthesize first and second cDNA strands followed by purification. Perform end repair, A-tailing, and sequencing adapter ligation on purified cDNA. Select fragment size using AMPure XP beads, then enrich the cDNA library through PCR amplification.