title: Enterovirus RNA from wastewater
design: Enterovirus RNA was concentrated from 350 mL of wastewater using glycine buffer elution followed by polyethylene glycol (PEG 8000) and NaCl precipitation. Viral RNA was extracted using the Maxwell RSC Viral Total Nucleic Acid kit (Promega), reverse transcribed and amplified with primers 5NCR/CRE-R targeting the entire capsid coding region and part of the 5UTR. PCR products were purified, quantified by Qubit, and sequenced using Illumina MiSeq (paired-end 150 bp).