title: Genome sequencing of Serratia plymuthica
design: Cells of S. phymuthica SWSY-3.47 were grown at 30 °C in YEM medium containing 0.5% chitin powder (Junsei Chemical Co., Tokyo, Japan). Approximately 2.0 × 10^9 cells were collected, and the genomic DNA was purified using NucleoBond® HMW DNA (TaKaRa), following the manufacturer's protocol. Long read sequencing libraries were prepared and multiplexed using the Rapid Barcoding Kit (SQK-RBK114; Oxford Nanopore Technologies). Libraries were sequenced using a FLO-MIN114 Flowcell with GridION X5 device (GridION software release 21.05.25; Oxford Nanopore Technologies). Long reads were basecalled, demultiplexed, and trimmed for the adaptor sequences using guppy 6.4.6 (super accurate mode).