title: Custom dsDNA primer panel for virus detection in human metagenomic data
design: To detect viruses, we applied our customized primer panel for a series of dsDNA primers. We performed 25 PCR cycles using the KAPA HiFi HotStart ReadyMix (2X) (Roche Diagnostics, Zug, Switzerland). After purification of PCR products with the SPRI bins, we indexed 5 ng of the resulting amplicons with the KAPA HiFi HotStart ReadyMix (2X) (Roche Diagnostics, Switzerland) and the Nextera XT Index Kit (Illumina, USA). We ran 8 cycles of index PCR according to the Illumina protocol. The obtained libraries were sequenced using the Illumina MiSeq platform.