title: DNA sequencing of strain MYR-2 in Miyun Lake sediment
design: living MTB cells was amplified using the REPLI-g Single Cell Kit (Qiagen, Germany), following the manufacturers protocols. Amplification products were then purified, and small fragments (e.g., less than 4000 bp) were eliminated using the QIAEX II Gel Extraction Kit (Qiagen, Germany). Purified genome DNA was sequenced with an Illumina NovaSeq 6000 with the pair-end strategy of 150-bp reads with an average 270-bp insert size