title: WGS of vancomycin-resistant Enterococcus faecium strain VRE81
design: The genomic DNA was randomly sheared into shorter fragments. The obtained fragments were then end-repaired, A-tailed, and further ligated with Illumina adapters. The resulting fragments with adapters were size selected, PCR amplified unless otherwise specified as PCR-free, before proceeding for purification. The library was quantified through Qubit and qPCR, and size distribution detected with fragment analyzer. Quantified libraries were pooled and sequenced on Illumina NovaSeq X Plus platform.