title: RNA-Seq of Xanthomonas oryzae pv. oryzae strain N1
design: Total RNA was extracted from tissue samples and its quality assessed. rRNA was removed from total RNA to enrich mRNA for prokaryotes. mRNA was fragmented to approximately 200 bp, reverse-transcribed into cDNA, and second-strand cDNA containing uracil was synthesized. After end repair and A-tailing, adapters were ligated, and the second strand was digested using UNG enzyme. Libraries were PCR-amplified, quantified, pooled, and sequenced on a next-generation platform with paired-end reads (2150 bp/300 bp). This workflow ensures high-quality transcriptome analysis suitable for various biological samples.