title: Paired-end illumina sequencing of Shigella boydii
design: The Kapa Hyperplus kit was used to prepare the library. The nucleic acid was subjected to enzymatic fragmentation to produce dsDNA fragments. It was followed by end repair and A-tailing to produce end-repaired, 5-phosphorylated, 3-dA-tailed dsDNA fragments. This was followed by adapter ligation where dsDNA adapters with 3-dTMP overhangs are ligated to the 3-dA-tailed molecules. The generated library was then amplified with minimal PCR cycles to avoid bias. The amplified library is then subjected to clean-up usingmagneticbeads