title: Single-cell RNA sequencing of female Schistosoma mansoni after 2 days of DMSO treatment
design: virgin females from single-sex infections were placed into 12-well plates with 25 worms per well and cultured in 3 mL A169 medium supplemented with 50 M BATT. The first day of the experiment was recorded as day 0. Media were replaced every other day until harvest at day 2. As controls, worms cultured in parallel were treated with DMSO. Each sample included ~200 virgins and three biological replicates were performed in each group.