title: AMPLICON of sediment: Oct22, E 3-4
design: DNA from sediments and enrichment cultures was extracted using the DNeasy PowerSoil Kit from Qiagen GmbH (Hilden, DE). Sequencing was performed by Novogene Europe (Cambridge, UK) and their standard workflow was followed. The bacterial 16S V3-V4 region was targeted using the primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). Libraries were sequenced on a paired-end Illumina NovaSeq 6000 platform (San Diego, CA, US) to generate 250 bp paired-end raw reads.