title: Bioreactor metatranscriptome (Day 557)
design: RNA sample extracted on Day 557 was used for metatranscriptomic sequencing. Quantification and quality control of RNA samples were conducted with Qubit RNA broad range Assay Kit (Invitrogen) and Agilent RNA tapes (#5067-5576) on the TapeStation 4200 (Agilent # G2991AA), respectively. Then RNA-seq libraries were prepared using 16 ng total RNA input with Illumina Stranded Total RNA Prep with Ribo-Zero Plus (Illumina #20040529). To amplify and add indexes and primer sequences for sequencing, 17 PCR cycleswere carried out based on the library prep protocol. Library quantification, quality control, pooling, and sequencing were performed the same as described above for metagenomic sequencing.