title: Lasiodiplodia theobromae cultured on PDB medium treated with EC30 pyriofenone for 12 hours
design: Mycelial plugs (6 mm diameter) from the edge of 3-day-old colony of the strain CGMCC3.20151 was transferred to PDB medium and inoculated in a incubator at 28C for 36 h. And were treated at pyriofenone EC30 concentration for 12h. Mycelia were ground into fine powder under the protection of liquid nitrogen.  About 100 mg mycelia powder were used for RNA extraction. Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure.  The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) , high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library.