title: RNA-Seq of mus musculus: dendritic cells
design: DC cultures (n=3 per group) were harvested two days after infection, and RNA was isolated using the RNeasy Mini Kit according to the Manufacturers instructions (QAIGEN).  RNA integrity was assessed with an Agilent Bioanalyzer 2100 system (Agilent Technologies) with recorded RIN 7-9 values.  Stranded mRNA sequencing libraries were sequenced using the NextSeq 150 cycle kit (Illumina Inc) generating 75 bp paired-end reads.