GSM8594544: Human Colon Cancer and Normal Adjacent Tissue (Pool 3 Library 4); Homo sapiens; RNA-Seq
Cells were dissociated from 50 µm FFPE curls from CRC tissue samples (n=5 patients) and a subset of paired normal adjacent tissues (n = 3 patients) using the Demonstrated Protocol for Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling (CG000632) Flex library preparation followed the Chromium Fixed RNA Profiling for Multiplexed Samples User Guide (CG000527, RevD). Each sample was hybridized with a unique probe barcode in an individual reaction. After hybridization, samples were multiplexed in equal cell proportions, washed, and processed collectively, across multiple GEM lanes as technical replicates, using the Chromium X instrument.