RNA sequencing of tammar wallaby: d148 pouch young testis, sample ID "7927"
RNA prepared from snap-frozen tissue using the GenElute Mammalian Total RNA Miniprep Kit (Sigma-Aldrich, RTN70-1KT). Long-read PCR-cDNA libraries prepared with barcoding kit (ONT, SQK-PCB109) starting from 100 ng total RNA and 16 cycles of PCR with 2 min 30 sec extension time. 50 ng of a barcoded pool with sequencing adapters were loaded onto PromethION flow cell (FLO-PRO002, R9.4.1) and run on PromethION P24 (MinKNOW 21.10.8). Reads basecalled and demultiplexed with guppy 5.0.17 in super-accurate mode, without adapter trimming using q>10 for pass.