GSM8386324: Control brain tissue lysate Day2-day5 1; Drosophila melanogaster; RNA-Seq
RNA was isolated manually using Trizol . cDNA was prepared using SMART-Seq-v4 Ultra (Takara) Kit following manufacturer's protocols. Nextera XT DNA library kit (Illumina- FC-131-1024) was used for library preparation with 1ng of cDNA, following manufacturer's protocol.