Gene profiling of human iPS-ECs with monoallelic and biallelic mutation in HNF1A gene as compared to isogenic hiPS-ECs control cells
Gene profiling of human iPS-ECs with monoallelic and biallelic mutation in HNF1A gene as compared to isogenic hiPS-ECs control cells
Cells were detached with accutase, washed with PBS and lysed with Fenozol. RNA was isolated using Chomczynski method.  Ion AmpliSeq Transcriptome Human Gene Expression Panel was used for generation of the libraries.