Fondecyt 1240264 Exp 3 202
DNA extracted using bead beating and DNeasy PowerSoil Pro kit (Quiagen). PCR amplification of V3-V4 regions of archaeal rRNA gene. Illumina P5 and P7 adaptors attached to Illumina Nextera XT Index Kit v2 indices. Mixture was loaded in the Illumina platform at 2  300 Illumina paired forward and reverse cycles run with FastQ.