Proof of principle for iPS2-10X-seq: cardioids experiment during 3D cardiac differentiation
Proof of principle for iPS2-10X-seq: cardioids experiment during 3D cardiac differentiation
Digestion with versene and trippleE. Then cells were labeled with CMO, sorted for vital cells and then loaded on the 10X controller. 10X genomics technology with CG000388 Rev B (with cell multiplexing oligos). Cells were loaded on a chip G. CG000388 Rev B (with cell multiplexing oligos). hPS-2seq barcode enrichment protocol was performed with custom primers binding on the TET repressor.