GSM8594520: Episomal_MPRA_RNA3; Mus musculus; OTHER
DNA: Proteinase K digestion followed by isopropanol precipitation. RNA: extracted by Trizol and purified by Zymo RNA Clean and Concentrator column with DNAse digestion. RNA was reverse transcribed with Oligo-dT, reporter transcripts were selectively amplfied, and NGS adapters added during two rounds of PCR. NGS adapter addition by two sequential rounds of PCR