E004RNAseq4
Cells were collected from healthy donors or indicated patients with written informed consents, and were sorted on FACSAria II Cell Sorter (BD Biosciences). RNA from the tumor cells of the patients was extracted using TRIzol reagent (Invitrogen Cat# 15596026). RNA sequencing (RNA-seq) and processing were performed in Novogene Technology CO., Ltd., Tianjin, China. Using an Illumina Novaseq 6000 platform, sequencing was done in a 2150 paired-end (PE) configuration.