RNA-seq of mus musculus: Sertoli cell line
Library prep was done with the True Seq Standard Total RNA Library Prep Gold kit (Illumina) and experiments were run on a NextSeq 500 instrument using a High Output Flow Cell V2.5 cartridge (Illumina). Reads were trimmed using the R Trimmomatic tool with a sliding window average across four bases with minimu quality of 20. Paired reads were aligned to the reference mouse genome GRCm38 (mm10) using R HISAT2.