GSM9049851: Cell YNBG, 0 h, rep 3; Debaryomyces hansenii CBS767; RNA-Seq
Total RNA was extracted using the Schmitt et al. (1990) hot phenol method in AE buffer (50 mM sodium acetate, 10 mM EDTA, pH 5.2). RNA quality was checked on denaturing agarose gels. Poly(A)+ mRNA was isolated using oligo(dT) beads, fragmented, and reverse transcribed. Libraries were prepared by end repair, adapter ligation, size selection (~150 bp), and PCR, Novogene Co.