GSM9598336: 94_DEN_T_CA_4; Mus musculus; RNA-Seq
The mouse liver and liver tumour tissues were snap frozen in liquid nitrogen and stored at −80°C. Total RNA from liver or tumor tissues was extracted using Favor Prep Total RNA Mini Kit (Favorgen, Cat. FABRK 001) with DNase I treatment (Qiagen, Cat. 79256) according to the manufacturer's instructions and stored at −80°C until use. The RNA-seq libraries were prepared from 100 ng total RNA of liver or tumor tissues using Truseq stranded mRNA library prep kit (Illumina, Cat. 20020595). The libraries were sequenced with signle-end 100bp on NovaSeq 6000 (Illumina) to yield a median of 20 million reads per sample.