NextSeq 2000 sequencing
Low-input DiCLIP CATL library from 6-well HUVEC culture, anti-HuR, biological replicate 1.
Low-input DiCLIP libraries were generated from formaldehyde-fixed HUVECs cultured in 6-well plates. Lysates were incubated without RNase inhibitors to allow endogenous trimming, then subjected to anti-HuR or control IgG immunoprecipitation, papain-mediated elution, de-crosslinking, and RNA purification. Libraries were prepared with the BioLiqX Small RNA-Seq Kit (HB202A) using the CATL semi-ligation workflow and sequenced as single-end 150 nt reads on an Illumina NextSeq 2000.