RNA-seq of onion:sterile pollen
Total RNA from flower buds with three biological replicates of MS and MF plants were extracted using TRIzol reagent (Thermo Fisher Scientific, MA, USA) following the manufacturer's procedure. The quantity and purity of RNA were assessed using the NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA), and RNA integrity was assessed by the Bioanalyzer 2100 (Agilent, CA, USA). Subsequently, samples that met the quality criteria were processed for library construction and sequencing on an Illumina NovaseqTM 6009 platform by LC-Bio Technology CO., Ltd. (Hangzhou, China).