GSM9101219: DENV2, Native, TMO modified Rep 1; Orthoflavivirus denguei; OTHER
Dengue virions were collected from infected cells and then sujected to a sucrose gradient followed by ultracentrifugation. The resultant fraction was collected and RNA extraction was performed using Trizol. MaP-RT was performed using random priming. Two rounds of PCR were performed to add Illumina adapter sequences and barcodes.