GSM8927042: X1249 H3K27ac; Mus musculus; ChIP-Seq
Mouse epidermis single cell suspension Crosslinked samples by addition of formaldehyde (Fisher chemical, F79) to 1% and incubating for 10min at RT at 1000rpm in thermomixer. Quenched crosslinking reaction by the addition of glycine (Fisher scientific, BP381) to 125mM and incubating at 1000rpm for 5min at RT. Centrifuge at 1200rpm for 5min at 4°C. Resuspend pellet with 1mL cold DPBS (Gibco, 14190-136) and centrifuge at 1200rpm for 5min at 4°C. Remove supernatant and store cell pellet at -80°C; (n = 3 for all groups, WT females, Utx-cKO females). Primary antibodies used: H3K27ac (Abcam, ab4729); : UTX (D3Q1I) Rabbit mAb (#33510, Cell signaling) Lot 4.