GSM8815445: T12.1 StnI100nM 12h replicate 1; Homo sapiens; RNA-Seq
The cells were then collected, washed with PBS, and RNA extraction was performed within 2h post lysis using Qiagen RNeasy kit (QIAGEN, Venlo, The Netherlands) according to manufacturer's directions. RNA integrity was checked in 2% agarose gels. RNA purity and concentration were assessed using NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA USA). The RNA samples were sent for sequencing to Biomarker Technologies (BMKGENE, Beijing, China) Library preparation for Transcriptome sequencing. A total amount of 1 μg RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, Shanghai) Co., Ltd.) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.