GSM8885766: Arabidopsis nac42-L1 (SALK_016619C) grown in mediterranean climate infected by S. sclerotiorum replicate 3; Arabidopsis thaliana; RNA-Seq
The border (ring of ~5mm wide) of disease lesions were collected with a scalpel at 30 hours post inoculation (hpi) for temperate and mediterranean acclimation and 48 hpi for continental acclimation,  and frozen in liquid nitrogen. Samples were ground with metal beads (2.5 mm) in a Retschmill apparatus (24hertz for 2x1min). RNA was extracted using the RNAplus kit (Macherey Nagel) following the manufacturer's instructions. A Turbo DNAse treatment (Ambion) was applied to remove genomic DNA. The quality and concentrations of RNAs preparations were assessed with an Agilent Bioanalyzer using the Agilent RNA 6000 Nano kit HiSeq SBS Kit v4