Amplicon seq of CH4 biofiltration biofilm
16S rRNA gene amplicon sequencing of the V3-V4 hypervariable regions was performed by Massey Genome Services (Massey University, A-NZ). The single step polymerase chain reaction (PCR) method was used to prepare the amplicon library using Massey Genome Services dual index PDC primers which flank either side of the V3-V4 hypervariable regions (16Sf V3: CCTACGGGAGGCAGCAG and 16Sr V4: GGACTACHVGGGTWTCTAAT).