16S amplicon data from Carex aquatilis rhizosphere planted in oil sand process affected water
For the rhizosphere, fine roots were transferred into 50-mL Falcon tubes containing ~ 10-15 mL of PowerBead solution (QIAGEN). Tubes were shaken to dislodge the rhizosphere weakly attached to roots, placed in a bath sonicator (Branson 2210 ultrasonic cleaner; frequency of 40 kHz) for 30 sec and centrifuged at 4 C 4,500 rpm for 10 min. Two grams of roots were transferred into new 50-mL Falcon tubes containing fresh PowerBead solution, while the remaining pellets of rhizosphere were kept on ice. Isolation of DNA was performed with the DNeasy PowerSoil Pro kit. Prokaryotes were targeted by sequencing the V4-V5 region of the 16S rRNA gene  with the 515F-Y  and 926R primer pair.