RNA-seq of Epidendrum fulgens: adult flowers
The samples were prepared using the Illumina Truseq stranded mRNA library prep, this kit uses oligo dT coated magnetic beads to enrich mRNA by picking out polyA tails, the method of first and second strand synthesis allows for strand identification. The resulting libraries have unique indexes to allow identification of each sample. Libraries were then normalized, pooled equimolar and sequenced on a Miseq nano cartridge PE 100bp for each read. According to the Illumina dilute and denature protocol for loading. The pool was rebalanced based on the Miseq results and the final pool was sequenced on an S4 200 cycle Novaseq cartridge PE 2x100, accessed via Wales Gene Park. The target read depth for each sample was 100M reads.