Foxo Het reporter negative rep 3
cDNA synthetised from RNA extracted with the Qiagen AllPrep DNA/RNA micro kit using Invitrogen SuperScript II Reverse Transcriptase and Template Switching Oligo, followed by full-length amplification using Roches KAPA HiFi HotStart & IS-PCR primer. Libraries were prepared using Nextera XT DNA with DNA/RNA UD indexes Set A & Tagmentation kits.