GSM9458439: Upstream tandem hiPSC, induced, rep2; Homo sapiens; OTHER
Cells were bound to ConA beads alongside spike-in Drosophila nuclei, permeabilized, and bound with an anti-EGFP nanobody fused to MNase. The MNase was activated after washing. Processed as in CUT&RUN, indexed libraries were prepared following the NEB Ultra II DNA Library Prep kit.