Pacbio-Seq of Callicarpa nudiflora: young leaf
A total of 2 ug of DNA per sample was used for library preparation. Genomic DNA was sheared using g-TUBEs (Covaris, USA) to the desired fragment size. The DNA fragments were then repaired, end-polished, and ligated with stem-loop adaptors for PacBio sequencing. Unligated fragments were removed with exonuclease, and target fragments were selected using BluePippin (Sage Science, USA). The SMRTbell library was purified with AMPure PB, and its fragment size was analyzed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Sequencing was performed on a PacBio Sequel II instrument with the Sequel II Sequencing Kit 2.0 at Nextomics.