RNA-Seq of Treatment-Resistant Asthma Human Sputum
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) and quantified with the Qubit fluorometer (Life Technologies). RNA-seq libraries were then generated using the Ion Total RNA-Seq Kit v2 (Life Technologies) and amplified. Finally, templates were prepared with the Ion 540 Chip Kit (Life Technologies) and loaded onto the Ion Torrent platform for sequencing.