16S amplicon data from oil sand process affected water
Water samples were collected from the mesocosms using sterile Nalgene bottles and shipped on ice to the University of Calgary where they were filtered through a 0.2 m a PES membrane filter prior to DNA extraction with the DNeasy PowerSoil Pro kit (QIAGEN) following the manufacturer's instructions. Isolation of DNA was performed with the DNeasy PowerSoil Pro kit. Prokaryotes were targeted by sequencing the V4-V5 region of the 16S rRNA gene  with the 515F-Y  and 926R primer pair.