WGS of Candida spp.
For extraction of genomic DNA, cells from single colonies of each candidemia isolate were picked and grown overnight at 30C in four ml cultures of YPD broth (1% [wt/vol] Difco yeast extract, 2% [wt/vol] Bacto peptone, 2% [wt/vol] dextrose) with shaking. DNA was then extracted with the Qiagen Genomic-tip 100/G using the Qiagen genomic buffer set (Qiagen, Hilden, Germany), following the manufacturers yeast protocol. Cell wall digestion was accomplished with lyticase (catalog number L2524; Sigma-Aldrich St. Louis, Missouri, USA). Library preparation and 2x150 bp paired end sequencing was performed using the Illumina NovaSeq platform.