RNA-Seq of Wisteria sinensis Sims affected by WMD
Following purification and digestion with DNase I and RNase A, dsRNAs were reverse-transcribed and cDNA was used as a template to perform a PCR-based random amplification. MID-indexed libraries were prepared using the TruSeq Stranded mRNA kit and sequenced in multiplexed format (2x150 bp paired-end) on a Nextseq 500 system (Illumina Inc.).