Sus scrofa ovarian granulosa cells transcriptome profiling by RNA-Seq
This study employed a controlled experiment to investigate the transcriptomic response of porcine ovarian granulosa cells to dietary -carotene supplementation. A total of 20 healthy replacement gilts at 130 days of age were randomly allocated into two dietary groups: a control group (basal diet) and a -carotene-treated group (basal diet supplemented with 10 mg/kg -carotene) for a 60-day trial. Granulosa cells were collected from 4 biologically independent gilts per group at the expected puberty age of 190 days. Strand-specific, paired-end RNA sequencing was performed on the Illumina NovaSeq 6000 platform.