16S amplicon data from sediments in oil sand process affected water
Sediment, rhizosphere, and root samples were thawed at 4 C for 72 hours and poured into Petri dishes placed on ice. The roots were sorted while preserving the soil tightly attached to them (rhizosphere). Then, the remaining sediments were thoroughly homogenized, transferred into PowerBead Pro tubes (250 mg), provided with the DNeasy PowerSoil Pro kit (QIAGEN), frozen in liquid nitrogen and stored at -80C.Isolation of DNA was performed with the DNeasy PowerSoil Pro kit. Prokaryotes were targeted by sequencing the V4-V5 region of the 16S rRNA gene  with the 515F-Y  and 926R primer pair.