GSM9156623: Oocyte, 585_514, control; Ovis aries; Bisulfite-Seq
Genomic DNA was extracted from sperm samples using the Quick-DNA Miniprep Plus (Zymo Research). Oocyte and embryo samples were processed for whole-genome bisulfite sequencing (WGBS) using the EZ DNA Methylation Direct Kit (Zymo Research, Irvine, CA, USA). Following bisulfite conversion, library preparation was performed using the Pico Methyl Kit (Zymo Research).