JDM,lesional skin,enrollment
      For mRNA extraction from tape strips, we placed individual tape strips in Eppendorf tubes and added RNeasy Lysis (RLT) buffer containing 2% beta-mercaptoethanol to the first tube, followed by three ultrasonication cycles (cycle: 30 seconds on, 30 seconds off) using the Bioruptor Pico sonication system in the cold room. First tube lysate was transferred to the second tube, and sonication was repeated, continuing until we pooled lysate from all 20 tape strips. mRNA was subsequently isolated from the pooled tape strip lysate using the QIAGEN RNeasy plus mini kit. In sequential thawing batches, mRNA was isolated from BD PAXgene RNA tubes using PAXgene blood RNA kits following the manufacturer's instructions. The SMARTer Stranded Total RNA-Seq kit v3 – Pico Input Mammalian was used to generate RNA-seq libraries. Illumina sequencing was performed by the University of Michigan Advanced Genomics Core (AGC) using the NovaSeq platform and targeting 20 million reads per sample. 