GSM9196959: HUVEC, siNC, 2day, rep3; Homo sapiens; RNA-Seq
Total RNA was extracted from sorted endothelial cells or HUVECs using TRIzol reagent, with quality assessed by NanoDrop 2000 and Agilent 2100/4200. For RNA-seq, libraries were constructed by enriching mRNA with polyT, synthesizing cDNA, adding adapters, and amplifying via PCR.