GSM9017765: mESC,H2AZ1KIflag,3; Mus musculus; OTHER
Cells were washed with PBS and permeabilized using the reagents provided in the Vazyme CUT&Tag Assay Kit (Cat# TD904), following the manufacturer's protocol. Primary antibody was incubated with the cells overnight at 4°C. After washing, cells were incubated with the secondary antibody according to the kit instructions. Following tagmentation using the Vazyme CUT&Tag Assay Kit (Cat# TD904), DNA was purified and used for library construction with the Vazyme VAHTS Universal DNA Library Prep Kit for Illumina (Cat# TD202), according to the manufacturer's protocol. Libraries were PCR-amplified with indexed primers, size-selected, and sequenced on an Illumina platform.