GSM9197038: DP_TT-seq_rep1; Mus musculus; OTHER
TTchem-seq on sorted methylation reporter populations was performed following an established protocol (Gregersen et al., 2020). Briefly, cells were labelled for 10 min using 2 mM 4SU, total RNA was isolated and fragmented and streptavidin coated MicroBeads were used to enrich the 4SU‐biotin labeled RNA KAPA RNA HyperPrep kit (Roche) with dual indexing adapters was used to prepare the libraries which were sequenced on a NextSeq 550.