dRNA-Seq of S. cerevisiae
Total RNA was extracted using NEB Monarch RNA Isolation Kit (T2110S). Agilent TapeStation was used for RNA quality control. 1 g total RNA was used for the sample preparation using Oxford Nanopore Direct RNA Sequencing kit (SQK-RNA004) following manufacture protocol. The sample is then sequenced using MK1B MinION with FLO-MIN004RA flow cell.