GSM8920574: Heart ventricle, Nr4a2-icTg mouse 2, spikein, NR4A2 (ChIP); Mus musculus; ChIP-Seq
ChIP was performed using 30 µg of chromatin precleared with protein A agarose beads and 5.5 ug of antibody. 200 ng of D. melanogaster chromatin and 0.4 µg of drosophila-specific H2Av antibody (Active Motif; Cat. no. 39715) were added to each reaction for normalization purposes. Complexes were washed and eluted from beads with SDS buffer. ChIP and pooled chromatin input samples were subjected to RNase and proteinase K treatment. Crosslinks were reversed by incubation at 65°C and DNA was purified. ChIP-seq libraries were prepared using the DNA Library Prep Kit for Illumina (Active Motif).