GSM8965564: Calu3 cells - Urolithin B - 48hrs - rep 1 (Drug study); Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) for 5–10 minutes, followed by storage at -80°C. RNA was purified using the Bio-Rad RNA Mini Kit and quantified using NanoDrop 2000. Libraries were prepared using the KAPA Stranded mRNA-Seq Kit. mRNA was selected, followed by cDNA synthesis, end repair, adaptor ligation, and PCR amplification. Libraries were multiplexed and sequenced using Illumina NovaSeq 6000 with 2×50 bp paired-end reads.