GSM9635390: Wildtype K562, sample 1, untreated, replicates 1 and 2; Homo sapiens; RNA-Seq
RNA was isolated using Trizol and quantified using RNA HS assay kit (Thermofisher) on Qubit flurometer (Thermofisher). The library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760S/L). Final libraries were quantified using Qubit 4.0 fluorometer (Thermofisher #Q33238) using DNA HS assay kit (Thermofisher #Q32851) following manufacturer's protocol.