GSM9049490: INTS11 depletion 24hrs, INTS11-degron mESCs rep1; Mus musculus; RNA-Seq
Total RNA was extracted using TRI Reagent and ethanol precipitated. 10 µg of total RNA was further processed to remove the remaining DNA with 4 U of TURBO DNase for 30 min and column purified prior to library construction. Total RNA libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold (Illumina, 20020598) following manufacturer's protocol.