GSM8449014: EBs 144h WT rep3; Mus musculus; RNA-Seq
144h EBs were dislodged by TrypLE, and alive cells were isolated by FACS. Cellular suspension was counted manually with a hemocytometer, and visualized under a ZEISS Primovert Microscope. Cells were resuspended in PBS + BSA 0.04% to a final concentration of 700-1200 cells/μl per sample.  Number of cells encapsulated varied from 10000 to 14000 depending on the sample, to achieve a final target cell recovery of 5000-7000 cells. Library was performed according to the manufacturer's instructions (single cell 3' v3 protocol, 10x Genomics). Resulting libraries were quantified and quality checked by using the 2200 TapeStation® (Agilent).