GSM9699927: BAP1, WT, MLL4, rep1, CUT&Tag; Mus musculus; OTHER
Cells were washed and harvested by wash buffer and incubated with activated conA beads. Then primary antibody was added, followed by Guinea Pig anti-Rabbit IgG antibody. After incubation, pA-Tn5 adapter complex was added. The purified DNA was amplified, then 200-1,000bp fragments were enriched for sequencing. CUT&Tag libraries were prepared by Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyem, TD904)