GSM9100532: Colon content HFD, rep 6; Rattus norvegicus; OTHER
Total genomic DNA was extracted using the QIAmp PowerFecal Pro DNA Kit, 10 ng of DNA was used for library preparation Sequencing of pooled libraries, spiked with 10% PhiX control library, was performed at 600 K reads/sample in paired-end mode with 2x150 nt read length on the MiniSeq platform (Illumina). For this, custom primers were spiked into the Illumina primers for both, normal and index reads.