GSM9196533: Multiome, Patient 3, RCC, ATAC; Homo sapiens; ATAC-seq
Single Cell Multiome ATAC + Gene Expression was performed with 10X genomics system using Chromium Next GEM Single Cell Multiome Reagent Kit A (catalog no. 1000282) and ATAC Kit A (catalog no. 1000280) following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide and demonstrated protocol - Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing. Cells were lysed for 4min and resuspended in Diluted Nuclei Buffer (10x Genomics, PN- 2000207). Lysis efficiency and nuclei concentration was evaluated on Countess II automatic cell counter by trypan blue staining.  Nuclei were loaded in the transposition reaction, targeting recovery of 10,000 nuclei after encapsulation. After transposition reaction nuclei were encapsulated and barcoded.