GSM8385880: HBV-replicate-PBMC1(Tag07mm), HBV-replicate-PBMC2(Tag08mm),scRNAseq; Mus musculus; RNA-Seq
Intrahepatic mononuclear cells (MNCs) were isolated from perfused livers using 10mL PBS followed by filtering through a 70 μM cell strainer and gradient centrifugation on Percoll (40% and 70% Percoll, #17089101; Cytiva). Mononuclear cells from spleens were also filtered through a 70 μM cell strainer (#352350; Corning, NY, USA) and resuspended in red blood cell (RBC) lysis buffer. For scRNA-seq, we utilized the BD Rhapsody system (Becton Dickinson). MNCs were labelled with sample tags (Ms Single Cell Sample Multiplexing Kit), counted, and multiplexed. Cell capture and library preparation were performed using the BD Rhapsody Whole Transcriptome Analysis (WTA) Reagent Kit (Becton Dickinson) following the manufacturer's protocol, and libraries were sequenced on MGI Tech DNBSEQ-G400RS.