RMC75_EOT_RNA
RNA purity was assessed for quality and then fragmented, and reverse-transcribed into first- and second-strand cDNA followed by adapter ligation. A total of 200 ng of the prepared library was hybridized with biotinylated probes (SureSelect XT HS Human All Exon V8 probes) and captured using streptavidin-coated magnetic beads. Libraries were amplified via PCR, after which the final libraries were normalized and pooled to a 6 nM concentration and sequenced on the Illumina NovaSeq 6000 system. Sequencing runs were done using dual-indexed paired-end read format with a total of 220 cycles