GSM8885760: C15 clone repeat 3; Homo sapiens; RNA-Seq
Total RNA was isolated with RNAeasy (Qiagen) and quality controlled using a Qubit instrument. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.