GSM8594164: Ctl lung, HDM/OVA; Mus musculus; RNA-Seq
On embryonic day 14, either PBS or LPS was administered to the pregnant mice. When the offspring reached postnatal day 10, they were sensitized and challenged intranasally with HDM and OVA. Lungs were harvested, and the tissue was digested with collagenase IV and DNase I, followed by filtration through a 70 μm strainer to obtain a single-cell suspension. From the lung cells, 5,000 CD4- and 5,000 CD4+ cells were isolated using FACS from the CD45+ cells. Library was contracted with 10x genomics Chromium Next GEM single cell 5` V2 kit accorting to the manufacterer`s recommendations.