GSM8889335: BPRNF SI Tum, replicate 3; Mus musculus; RNA-Seq
Samples were homogenised and digested using a McIlwain Tissue CHopper and the GentleMACS Octo Dissociator in combination with the Mouse tumor dissociation kit (Miltenyi Biotec). Debris was removed, and then cells were sorted using a FACSAria sorter (BD Biosciences) and DAPI to remove dead cells. Cells were loaded into a Chromium Chip G using a 10x Chromium Single-Cell 3' v3 Gel Bead kit and Library (10x Genomics), then libraries were analysed using the Bioanalyzer High Sensitivity DNA kit (Agilent technologies). Sequencing was performed on an Illumina NovaSeq 6000 with paired-end 150-base reads.