GSM9128374: Liver tumor, Day 21–28 post-Myc induction, replicate 2; Mus musculus; RNA-Seq
liver and tumor RNAs (five samples per time point) were purified using RNA Easy columns (Qiagen, Inc., Valencia, CA). RNA concentrations were determined with a Nanodrop ND-1000 instrument (NanoDrop Technologies Inc., Wilmington, DE), and RNA integrity was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA) The Sample preparation was replicated using Illumina TruSeq Stranded mRNA kit (San Diego, CA). mRNA was used to generate poly-A+ libraries using oligo-dT beads following 2 rounds of poly-A selection