GSM8449344: RNA-Seq - KPL4 cells, ERβ-KO + exogenous ERβ, replicate 3; Homo sapiens; RNA-Seq
Total RNA was isolated using the Qiagen RNeasy Mini kit (Qiagen) and quantitated in NanoDrop 1000 spectrophotometer (Thermo Fisher). The quality of RNA was evaluated by capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies). Libraries for total RNA were prepared using the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus (Illumina)