GSM8889078: iPSC-derived cerebral organoids, CEP290 CRISPR gene edited, clone 2, day 79; Homo sapiens; RNA-Seq
Three whole cerebal organoids were pooled washed three times in HBSS per sample. Dissociation was performed using the gentleMACS™ Dissociator with the Neural Tissue Dissociation Kit in C tubes. The enzyme mix was stopped by adding 5 mL of HBSS + 5% FBS after dissociation and in between rotation steps. Cell suspension was passed through a 40 μM cell strainer and FACS tube strainer. Lastly, ultra-slow centrifugation at 90 g for 10 min was performed. Library was constructed according to the 10X Genomics protocol.