16S amplicon sequencing of skin nasal and Rectal bacteria in early stage baby
DNA was extracted from samples using the DNEasy PowerSoil Pro Kit (Qiagen) according to the manufacturer's protocol. Barcoded PCR primers annealing to the V1-V2 region of the 16S rRNA gene were used for library generation. The resulting library was sequenced on the Illumina MiSeq using 2x300 bp chemistry.