RNA-Seq of anaerobic sludge mixture
DNA isolation was performed with the DNeasy PowerSoil Pro Kit (QIAGEN, Germany). PCR amplification of 16S rRNA gene V4 and V5 hypervariable regions was carried out with 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 907R primers (5'-CCGTCAATTCCTTTGAGTTT-3').