genome-wide CRISPR/Cas9 knockout (GeCKO) screening of BEAS-2B cells treated with TGF-beta1
BEAS-2B cells were transduced with GeCKO v2 sgRNA library to generate the BEAS-2B-GeCKO cells. Cells were then treated with TGF-beta1, followed by Genome-wide CRISPR/Cas9 knockout (GeCKO) screening. We analyzed two samples (generation of BEAS-2B-GeCKO cells and TGF-beta1 treatment) using the Illumina next-generation sequencing. Genomic DNA was extracted from TGF-1-resistant BEAS-2B-GeCKO cells. The gRNA sequences and single guide RNA sequences were amplified by polymerase chain reaction. The distribution of sgRNA was assessed by Illumina next-generation sequencing.