Proof of principle for iPS2-10X-seq: timecourse experiment during 2D cardiac differentiation
Proof of principle for iPS2-10X-seq: timecourse experiment during 2D cardiac differentiation
Single cell suspension obtained after harvesting cells with versene. 10X protocol with 10X user guide CG00315 Rev E. 1.6 x 10^4 cells were loaded on a 10X chip G of a 10X 3' v3 kit. 10X user guide CG00315 Rev E for the cDNA and library construction of the gex library. The Barcode library follows the iPS2-seq protocol enrichment with a custom primer on the TET repressor.