GSM9048868: ein2-5, untreated, rep1; Arabidopsis thaliana; RNA-Seq
Total RNA was isolated according to Logemann et al. (1987) and contaminant DNA was removed with the TURBO DNA-free kit (Thermo Fisher). 250 ng per sample were used for sequencing mRNA was isolated with poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. After end repair, A-tailing and adapter ligation, size selection, amplification, and purification, the libraries were checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and paired-end sequenced on the Illumina platform NovaSeq X Plus (PE150).