GSM8964977: pigMLO_D28_A; Sus scrofa; RNA-Seq
Single-cell suspensions were processed using the Chromium X system and GEM-X Single Cell 3' v4 Kit (10x Genomics, CG000731). Libraries were constructed via reverse transcription, cDNA amplification, adapter ligation, and PCR enrichment. Quality was assessed with qPCR and Agilent TapeStation. Single-cell RNA sequencing (scRNA-seq) libraries were prepared using the Chromium X platform following the 10x Genomics GEM-X Single Cell 3' Reagent Kit v4 protocol (CG000731).