GSM9423113: Liver_WT_653, Male; Mus musculus; RNA-Seq
Total RNA was isolated using the RNeasy Plus Micro Kit (QIAGEN, 74034) according to the manufacturer's instructions. Snap-frozen tissues were homogenized in the lysis buffer provided in the kit using a bead-based tissue homogenizer. RNA quality was assessed on an Agilent TapeStation at the CCR Genomics Core, National Cancer Institute. RNA-seq libraries were prepared from total RNA using Illumina Stranded mRNA Prep and sequenced on an Illumina NovaSeq X Plus platform using paired-end sequencing at the Frederick Sequencing and Genomics Core.