whole-exome sequencing of mus musculus: adult female spleen
Samples were snap frozen and sent to Novogene Ltd for DNA extraction and whole exome sequencing (WES). Briefly, genomic DNA was sheared into fragments (180-280 bp), repaired, A-tailed, and ligated with Illumina adapters. After PCR amplification, size selection, and purification, libraries were hybridized with biotin-labeled probes to capture gene exons. Non-hybridized fragments were removed, and the captured libraries were enriched via PCR. Quantification and size distribution of the libraries were assessed using Qubit, real-time PCR, and a bioanalyzer. Libraries were pooled based on concentration and sequenced on Illumina platforms using the PE150 strategy