GSM8787541: MIA PaCa-2 shCLPX-1 rep2; Homo sapiens; RNA-Seq
MIA PaCa-2 shNS and shCLPX-1 cells were collected and lysed using TRIzol reagent (Invitrogen) according to the manufacturer's instruction. All samples were frozen at -80 °C before RNA extraction and analysis. The following steps were conducted by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China). RNA-seq transcriptome library was prepared following TruSeqTM RNA sample preparation Kit (Illumina) using 1 mg of total RNA. Shortly, messenger RNA was isolated according to the polyA selection method using oligo(dT) beads and then fragmented by fragmentation buffer. Next, double-stranded cDNA was synthesized using a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina). The synthesized cDNA was subjected to end-repair, phosphorylation, and “A” base addition according to the library construction protocol.