RNA-Seq of Mus musculus: heart muscle tissue from male mice treated with atorvastatin, replicate 2
Total RNA was extracted using the RNAeasy Mini Kit (Qiagen, Germany). Poly(A)+ mRNA was isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, USA). cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit, followed by purification using NEBNext Sample Purification Beads. The libraries were pooled and sequenced on the NovaSeq 6000 (Illumina, USA).