GSM9127482: F123_mESC_H3K27ac_Ab Biological repliacate 1; Mus musculus; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 mins and lysed with nuclear lysis buffer containing NP-40. Chromtain was then sheared with a Covaris. Sonicated chromatin underwent immunoprecipitation with antibody of choice then DNA was purified. NEB II Ultra DNA library prep kit was used for library prep with the ChIP DNA.