GSM8992134: mES-E14-NANOG-FKBP-dTAG_Rep2; Mus musculus; RNA-Seq
For each of the three replicates, 5x10⁷ cells were incubated with 500 μM 4-thiouridine (Enzo/Jena Bioscience, NU-1156S) for 5 minutes, followed by pelleting and lysis in Trizol (Thermofisher, 15596026). cDNA synthesis and sequencing library preparation were done using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760), following the protocol for purified mRNA.