GSM9422747: HTX-33_WT_3, control; Komagataella pastoris; RNA-Seq
Total RNA was extracted from tissue samples, and its concentration and purity were measured using a Nanodrop 2000. RNA integrity was assessed by agarose gel electrophoresis, and the RQN value was determined using an Agilent 5300 system. For each library construction, the RNA requirements were as follows: total amount 1 μg, concentration 30 ng/μL, RQN > 6.5, and OD260/280 ratio between 1.8 and 2.2 RNA-seq transcriptome library was prepared following Illumina ® Stranded mRNA Prep, Ligation (SanDiego, CA) using 1μg of total RNA.