GSM8539481: STm LT2, MUC2, 3 h, Sequencing Run 1, Bio Rep 1; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; RNA-Seq
RNA was extracted with the MasterPure RNA Purification Kit (Biosearch Technologies), and DNA was eliminated using the Turbo DNA-Free Kit (Ambion). The Agilent 2100 Bioanalyzer (Agilent Technologies) was used to assess RNA integrity. rRNA was depleted with the NEBNext rRNA Depletion Kit for bacteria (NEB). The Illumina HiSeq platform was used for the sequencing mucin-, glycan-treated, and monosaccharide pool-treated samples with a single-end protocol and read lengths of 40 nucleotides. The Illumina NovaSeq platform was used for sequencing HexNAc-treated samples with a paired-end protocol and read lengths of 50 nucleotides.