GSM8711489: PBS_1_D7; Mus musculus; RNA-Seq
Total RNA was extracted from colon tissue previously washed from luminal content. Samples were homogenized in TRIZOL. Homogenized tissues were then added with chloroform in order to separate RNA from genomic DNA and proteins. RNA isolation and purification required isopropanol (RNA precipitation) and 70% ethanol (RNA wash). Total RNA was resuspended in Nuclease-free water. Illumina standard protocol