GSM9422931: Proestrus rep3 snMultiome(ATAC); Mus musculus; ATAC-seq
Frozen ventral hippocampal tissue was homogenized and nuclei were isolated using a sucrose gradient-based protocol. Briefly, tissue was lysed in ice-cold buffer, layered over a sucrose cushion, and centrifuged to pellet nuclei. Isolated nuclei were washed, resuspended in nuclei buffer supplemented with RNase inhibitor, counted, and assessed for quality prior to library preparation. Single-nucleus multiome libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit according to the manufacturer's instructions. Isolated nuclei were loaded onto the Chromium controller to generate gel bead-in-emulsions (GEMs), enabling parallel barcoding of RNA and chromatin accessibility from individual nuclei. Separate gene expression and ATAC libraries were constructed and sequenced independently.