16S rRNA of Cephalothrix simula metagenome sample 20
C. cf. simula samples were dissected into 20 mg cross sections. Total genomic DNA was extracted from each sample using an E.Z.N.A. Mollusc DNA Kit (Omega Bio-tek, USA). A 16S rDNA metabarcoding approach was used to investigate the biotic diversity in C. cf. simula body wall. Universal eukaryotic primers were used to target the V3-V4 variable region of the 16S rDNA. The V3-V4 16S rRNA amplicon library preparation and Illumina NovaSeq 6000 paired-end sequencing (2x250 bp)