GSM8925987: Mouse 161 PBMCs, PEG+PBS, Whole Transcriptome (M6GEX); Mus musculus; RNA-Seq
Whole blood was spun at 1000 g for 10 min to collect the buffy coat followed by red blood cell lysis. Cells were then washed and assessed for viability. 16,500 cells per condition were loaded onto a 10X Chromium instrument (10X Genomics) per the manufacturer's instructions. Single-cell RNA and TCR libraries were generated using the Chromium Single Cell 5′ Library Construction (10X Genomics, Cat.# PN-1000190) and Chromium Single Cell Mouse TCR Amplification kits (10X Genomics, Cat.# PN-1000254) per user guide. Quality control of the completed libraries was performed using the Bioanalyzer High Sensitivity DNA Kit (Agilent, Cat.# 5067–4626) and then sequenced using the Illumina NovaSeq platform.