GSM8449007: CD45 BFP Bone marrow 1ary Transplant 4 weeks rep1; Mus musculus; OTHER
Genomic DNA (gDNA) from cells containing gRNA library (KDR or bone marrow cells) was extracted using Qiagen DNeasy Blood and Tissue Kits (Qiagen). gRNAs integrated into gDNA were amplified using TAKARA PrimeSTAR polymerase and library was constructed using the same TAKARA Prime Star enzyme Library construction was prepared as previously described in Joung et al. 2017 (https://doi.org/10.1038/nprot.2017.016). genomic DNA (gDNA) was sequenced using Illumina sequencer HiSeq 2500 (1x50bp)