GSM8449624: HCT116, anti-GFP, 250, replicate 3; Homo sapiens; OTHER
Cut&Run was performed as described by Skene et al., 2018 (https://doi.org/10.1038/nprot.2018.015). Each sample used 100,000 cells and protein A-MNase digest step used 40min incubation. Libraries were prepared using KAPA Hyper Prep Kit (Ref# 7962347001-KK8502) following the manufacturer's protocol. Adapters used were IDT for Illumina TruSeq UDI indexes (Ref# 20020178). Library amplification was performed using 13-16x cycles. Samples were sequenced on Illumina NovaSeq 6000 platform for PE150 sequencing.