GSM8787529: HC_NR_pos GEX; Homo sapiens; RNA-Seq
A total of 20 unique samples were hashed using BioLegend Total-Seq B antibodies. Cells were sorted and approximately 12,000 cells/sample were targeted and loaded on the 10X Chromium Controller (10X Genomics). We used the Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 (Dual Index) with Feature Barcode technology for Cell Surface Protein and Cell Multiplexing and performed cDNA synthesis, amplification, and library preparation for gene expression and hashtag antibodies according to manufacturer's protocol. cDNA and final libraries were quality checked for fragment size by capillary electrophoresis using the Tapestation 4200 (Agilent), and quantified using the Qubit Flex assay (Thermo Fisher), before pooling and sequencing on a NovaSeq 6000 S4 v1.5 Flowcell; Illumina) to a depth of > 20,000 reads/cell for gene expression and > 5,000 reads/cell for hashtag antibody libraries.