GSM9049485: WT1; Mus musculus; RNA-Seq
Cortex and hippocampus were dissected and homogenized in Lysis Buffer (EZ Prep Nuclei Kit, Sigma-Aldrich) with RNAase Inhibitor and filtered through a 30 mm MACS strainer. The samples were briefly centrifuged, washed and strained again before resuspended in washing buffer (10 mM Tris buffer pH 8.0, 5 mM KCl, 12.5 mM MgCl2, 1% BSA with RNAse inhibitor). The nuclei were then FACS sorted on a MoFlo Astrios Cell Sorter. The final nuclei suspension was adjusted to 750–1200 nuclei/μL. Sequencing libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell 3' RNA Library & Gel Bead Kit (v3) using the 10x Genomics Chromium X instrument  according to the manufacturer's protocol.