Tiled RT-PCR of SARS-CoV-2 from wastewater.
Total nucleic acid from Ceres Nanotrap concentrate of wastewater influent treated with TurboDNase, concentrated, reverse transcribed with LunaScript, amplified with ARTIC v5.3.2 primers using NEB Q5, converted into Illumina-compatible libraries using NEB Ultra II DNA kit, and sequenced on an Illumina system 300 PE (XLEAP-SBS) or 250 PE (standard SBS).