CD46 gene-editing confers ex vivo BVDV resistance in fibroblasts from cloned Angus calves
Dynamic pools of indexed libraries were sequenced with massively parallel sequencing machines (either NextSeq500 or NextSeq2000, Illumina Inc.) with the appropriate kits producing 2  150bp paired-end reads.  Pooled samples were sequenced until a minimum of 40 Gb of data was collected with greater than Q20 quality for each animal, thereby producing at least 13.3-fold mapped read coverage for each index (90% of reads mapped to a 2.7Gb reference genome).