ATAC-seq of d21 c13 Prdm1 KO TPEX
TPEX were sorted from spleens of LCMV-c13 infected mice on 21 dpi. Cells were treated in DNase for 30 minutes, followed by two washes in PBS. Nuclei were isolated for tagmentation according to the OmniATAC pipeline using the using the Illumina Tagment DNA TDE1 Enzyme and Buffer Small Kit (Illumina, Cat # 20034197) and indexed with the IDT for Illumina DNA UD Index Set D (Illumina, Cat # 20027213). Amplified libraries were cleaned up using double-sided bead purification with AMPure XP beads (Beckman Coulter, Cat # A63880).