16S rDNA from Corn leaves inoculated with Clavibacter nebraskensis and treated with 200 uL HEPES Buffer, replicate 1
PCR was used to amplify the V4 region of 16S rRNA. Sequencing was done by the University of Minnesota Genomics Center using barcoded primers and the dual indexing method. Amplicons were gel purified, pooled, and pairedend sequenced at a read length of 300 nucleotides on the Illumina MiSeq platform.