GSM8384307: N251_GT; Homo sapiens; OTHER
Nasal cells were stimulated with 1 μg/mL SARS-CoV-2 S OLP pools (PepTivator, SARS-CoV-2 Prot_S Complete; Miltenyi Biotec) and 1 μg/mL anti-human CD28 and CD49d mAbs (BD Biosciences) for 24 hours. Following stimulation, nasal cells were stained with PE-conjugated anti-CD3 antibody (BD Biosciences), APC-conjugated CD137 antibody (Miltenyi Biotec), and LIVE/DEAD near-IR fluorescent reactive dye (Invitrogen). And then live CD137+CD3+ T cells were sorted using an ARIA II instrument (BD Biosciences). Single-cell CITE-seq library was generated using the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) following the manufacturer's instructions. Libraries were constructed and sequenced at a depth of approximately 33,000 reads per cell for gene, antibody-derived tags (ADTs), and TCR sequence expression using the Novaseq 6000 platform (Illumina).