GSM9050260: MAS; Mus musculus; RNA-Seq
Complete blood count was performed on Hemavet 950 FS (Drew Scientific Inc). For scRNA-seq and cytokine release assays, peripheral blood neutrophils were isolated from whole 5 blood by magnetic negative selection with Neutrophil Isolation Kit, mouse (Miltenyi Biotec, Germany). Isolated blood neutrophils from 4 mice per conditions were pooled and resuspended at 700-1,200 cells/ μl in DPBS containing 0.5 U/μl of Protector RNase inhibitor (Roche, Basel, Switzerland ). 16,000 cells per sample were sequenced with 10x Genomics (Pleasanton, CA, USA) version Chromium NextGEM (Chemistry: 3'v3 Assay). Barcoded libraries were sequenced using the Illumina NovaSeq platform.