GSM8503012: P9 Tracheal, current-smoker; Homo sapiens; RNA-Seq
Single cell suspensions were obtained from the tracheal brushes as per previously described protocols (dx.doi.org/10.17504/protocols.io.qubdwsn) Single cell RNA libraries were prepared using 10x Chromium 5' V2 Gene Expression kit. The single cell suspension was loaded with the master mix, partitioning oil and gel beads into the chip to generate GEMs (gel bead-in emulsion), containing 10x barcoded cDNA from poly-A RNA. The pooled cDNA was then cleaned up using Silane Dynabeads, amplified by PCR and separated by size for 5' gene expression library construction.