GSM8413600: HeLa, siSPT6siPNUTS, rep1; Homo sapiens; RNA-Seq
Total RNAs were extracted using the ReliaPrepTM RNA Miniprep Systems (Promega, Z6012) as per the manufacturer's instructions. Sample quality was verified using the Bioanalyzer RNA 6000 Nano kit (Agilent) Libraries were prepared following the Stranded Total RNA Prep Ligation with Ribo-Zero Plus protocol from Illumina, starting from 200 ng of high-quality total RNA (RIN > 9). Paired end (2 × 59) sequencing was performed on an Illumina NextSeq 2000 platform. Demultiplexing and quality control were conducted with the AOZAN software (ENS, Paris) at the GENOM'IC platform, Institut Cochin.