GSM8787748: PRNE_NEPC_M4851; Mus musculus; Bisulfite-Seq
DNA methylation profiling was performed on biopsy punched tumor tissues of histopathologically confirmed foci of adenocarcinomas and NEPC from mouse primary tumor. DNA was extracted using Nucleospin Tissue kit (Macherey Nagel, 740952). For DNA methylation profile from patient-derived organoids, DNA was extracted from cell pellets of each. For EZH2 knockout lines, DNA was extracted from cell pellets after 14 days of nucleofection and validating the knockout. Library preparation for single-end 100 base-pairs(SE 100) RRBS libraries, sequencing, and post-processing of the raw data was performed at Epigenomics Core at Weill Cornell Medicine. The resulting libraries were normalized, pooled, multiplexed, and clustered on flow cells and sequenced for 100 cycles of single-end reads on an Illumina NovaSeq 6000 instrument.