RNA-Seq of bos taurus: fece group A number 6
DNA was extracted using the E.Z.N.A. DNA Kit. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using universal primers 338F and 806R.  PCR amplification was performed on an ABI 9700 PCR instrument. PCR products were purified using the Agencourt AMPure XP Kit. Sequencing libraries were generated using the NEB Next Ultra II DNA Library Prep Kit following the manufacturer's protocol.