18srRNA-seq of Tick
Total DNA was extracted using the NucleoSpin DNA Insect Kit (Macherey-Nagel, Dren, Germany), following the manufacturers instructions. The 16S rDNA V4 region was amplified by polymerase chain reaction (PCR) using the primers 515F401-TCGTCGGCAGCGTCAGATG343,22131207,22853700,18948169,17110992,22131208,22853699,7168707,22929425,5587867,5850525,23414153,8447777,244