WGS of Pomacea nobilis
Genomic DNA was sheared to an average fragment size of ~500 bp using a Covaris M220 ultrasonicator. Sequencing libraries were prepared following the blunt-end single-tube (BEST) protocol for double-stranded DNA, incorporating dual-indexed Illumina adapters during PCR amplification. Libraries were purified and quality-checked prior to sequencing. Equimolar pooled libraries were submitted to Novogene Europe for whole genome sequencing on an Illumina NovaSeq X platform, generating 150 bp paired-end reads.