GSM8919694: WT5_eWAT; Mus musculus; RNA-Seq
Total RNA was extracted using the RNeasy Mini Kit (Catalogue# 74104, Qiagen) following the manufacturer's instructions. The procedure involved homogenizing tissue samples in the provided lysis buffer to release RNA, followed by applying the lysate to RNeasy spin columns, where RNA binds to the column membrane. After several washing steps to eliminate contaminants, high-purity RNA was eluted in RNase-free water. RNA libraries for sequencing in DNBSEQ-G400 were prepared using the MGIEasy RNA Library Prep Set according to the manufacturer's protocol. This process involved extracting and enriching total RNA, followed by fragmentation, cDNA synthesis, and ligation of specialized adapters to prepare the samples for sequencing.