GSM8922351: PDO131 Patient-derived tumor organoids, RM-044, replicate2; Homo sapiens; RNA-Seq
RNA was extracted using the PureLinkTM RNA Mini Kit (Life Technologies, 12183025) and PureLinkTM DNAse (Life Technologies, 12185010) following the manufacturer's specifications. RNA was eluted using distilled water (Invitrogen, 10977-035) and the concentration was determined using the Nanodrop 1000 spectrophotometer (ThermoFisher) Libraries were prepared using RNA-seq Stranded mRNA Library Illumina and sequenced with Illumina NextSeq 2000 P3. Trim Galore (v0.0.6) was utilized for quality control and adapter trimming of FASTQ files