18S amplicon data from oil sand process affected water
Water samples were collected from the mesocosms using sterile Nalgene bottles and shipped on ice to the University of Calgary where they were filtered through a 0.2 m a PES membrane filter prior to DNA extraction with the DNeasy PowerSoil Pro kit (QIAGEN) following the manufacturer's instructions. Isolation of DNA was performed with the DNeasy PowerSoil Pro kit.  Eukaryotes were targeted by sequencing the V4 region of the 18S rRNA gene for the eukaryotes with the 565F and 948R primer pair.