GSM9050255: ACC67, dinaciclib, 24 h, biol repl 1; Homo sapiens; RNA-Seq
Total RNA was extracted using the RNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. cDNAs were then subjected to end repair, A-tailing, adapter ligation, size selection, amplification, and purification.