Transcriptomic analysis of lymphatic endothelial cells transfected with parasite Brugia malayi microRNA
Total RNA was extracted from the cells using the RNeasy Mini Spin Kit (Qiagen) following manufacturer instructions and used in conjunction with the Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina) for library preparation. Libraries were pooled, diluted, and quality-checked according to manufacturer specifications before loading onto a P3 type flow cell (Illumina) to obtain 151nt paired-end reads.