RNA-seq of human:cholangiocarcinoma subjects
The total exosomal RNA concentration was measured by spectrophotometer (ND2000c  Nanodrop, Thermo Scientific, MA) and miRNA concentration was measured using Qubit microRNA assay kits (Invitrogen, CA) using Qubit 4.0 Fluorometer (Invitrogen, CA). The integrity and quantities of exosomal RNA were determined using the Fragment Analyzer Automated CE System. RNA libraries were constructed from an equal amount of RNA from three distinct groups, utilizing the Ion Total RNA-Seq Kit v2