GSM8990519: Biological replicate of OV-1; Gallus gallus; RNA-Seq
Total RNA was isolated using Tri reagent (Sigma- Aldrich catalogue no:T9424) as per the manufacturer's protocol. Total RNA was resuspended in 12uL of DEPC treated water. 1uL of the sample was used for analysing the quality using bioanalyser and 1uL was used to check the concentration using Qubit. NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads (Catalog no-E7765L