DNA target sequencing AD patients
Genomic DNA was extracted using the QIAamp DNA extraction kit (QIAGEN, Germany) and subjected to next-generation sequencing (NGS) using a customized targeted panel encompassing 521 SNPs previously identified as loci of risk for AD was designed based on a comprehensive review of genome-wide association studies (GWAS) and AD-related variants curated from ClinVar and DisGeNET databases