GSM9017812: GD32-sh50-rep2; Homo sapiens; RNA-Seq
RNA was extracted from primary tumor and liver metastasis tissue by RNA Extraction Kit (Takara, 9597) following manufacturer's instructions. KI cell line CUT&RUN seq: CUT&RUN experiment was conducted following Skene et al's protocol (doi.org/10.17504/protocols.io.zcpf2vn) KI cell line CUT&RUN seq: Genomic DNA was extracted using the Blood & Cell Culture DNA Midi Kit (Qiagen, 13343). KI cell line CRISPR screen: CRISPR screen was conducted following zhang feng's protocal(doi: 10.1126/science.1247005). genomic DNA was extracted using the Blood & Cell Culture DNA Midi Kit (Qiagen, 13343). RNA-seq: 1 μg total RNA was used for following library preparation by VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (NR605). The libraries were sequenced on a NovaSeq 6000 System using 2x150 bp paired-end configuration. KI cell line CUT&RUN seq: Libraries were prepared using the Ovation Ultralow System V2 (Invitrogen, Q32854) KI cell line CRISPR screen: The sgRNA regions within the genomic DNA were amplified with Next® Ultra™ II Q5® Master Mix (NEB, M0544X) and sequenced  on the Illumina platform