GSM8965473: NC3; Homo sapiens; RNA-Seq
Total RNA was extracted from liver tissue using Trizol reagent (Servicebio, Wuhan, China). mRNA was then reverse-transcribed into cDNA using a reverse transcription kit (Servicebio, Wuhan, China). The SYBR Green (Biosharp, Wuhan, China) reaction system was loaded into a 96-well plate, and PCR amplification was performed according to the manufacturer's instructions. β-Actin was used as the reference gene. RNA was extracted from cell samples using the Trizol method and its integrity was assessed using the Agilent 2100 Bioanalyzer. mRNA was enriched via poly(A) selection, then fragmented and reverse-transcribed to synthesize cDNA. A cDNA library was constructed and high-throughput sequencing was performed on the RNA library using the Illumina sequencing platform to generate raw data