GSM8787758: HE_cont; Homo sapiens; RNA-Seq
Organoid dissociation was done by adding collagenase, pronase, and DNase at 37°C for 40 minutes in a water bath. Pipetting was carried out every ten minutes. RNA was extracted with PureLink™ RNA Micro Scale Kit (Invitrogen, Waltham, Massachusetts, USA) was used for RNA extraction from sorted cells. RNA libraries were prepared using total RNA with an Ion AmpliSeq Transcriptome Human Gene Expression kit (Thermo Fisher Scientific).