GSM8785686: FL514; Homo sapiens; RNA-Seq
Frozen liver tissues were processed to generate 10x Genomics-based snRNA-seq data in GENINUS (Seoul, Republic of Korea). Tissues were homogenized, and isolated nuclei was counted using flow cytometry (BD: FACSMelodyTM Cell sorter) 10x Genomics chromium instrument and cDNA synthesis kit v3 were used to generate barcoded cDNA libraries. cDNA library quality was determined using an Agilent Bioanalyzer.