RNA-seq of leaf tissue of Thinopyrum intermedium
Total RNA were collected from the leaf tissue of Thinopyrum intermedium. All fresh tissues were immediately frozen in liquid nitrogen to preserve RNA integrity. RNA extraction was performed for each sample using TRIzol Reagent (Invitrogen), following the manufacturers protocol. Paired-end RNA sequencing (RNA-seq) libraries were prepared according to the standard manufacturer instructions and sequenced on the DNBSEQ-T7 platform.