GSM8503701: Old replicate 4, snRNAseq; Mus musculus; RNA-Seq
Nuclei were extracted using the Nuclei EZ Prep kit (Millipore Sigma), following the manufacturer's protocol. Frozen hippocampi were homogenized in lysis buffer and centrifuged to twice to isolate the nuclei from the cell debris. The nuclei were resuspended in glycerol-containing buffer and stored at -80ºC. The libraries were prepped with the goal of 5000 nuclei per sample using the Chromium Single Cell 3′ Library and Gel Bead kit v3.1, Chromium Next GEM Chip G, and Dual Index Kit TT Set A (10X Genomics). single nuclei RNA-seq