Mus_musculus_hippocamp_Gld2_KO_rep3_Illumina
Total hippocampal RNA was extracted with RNeasy Lipid Tissue Mini Kit (Qiagen), purified with KAPA Pure Beads (Roche). RNA was ribodepleted with RiboErase (KAPA Biosystems) and the libraries were prepared from 1ug of RNA. The Libraries were fragmented by 5 min incubation at 94oC. The libraries were enriched with 9 amplification cycles. The quality was assessed with High Sensitivity D1000 Reagents on Agilent TapeStation 2200. The libraries were sequenced using Illumina NovaSeq 6000 sequencing platform in 2x100nt pair-end mode.