GSM8787065: Nox1 WT Repeat 3; Mus musculus; RNA-Seq
Lyse colonoids recovered from Matrigel in TRIzol™ Reagent. Then isolate RNA according to manufacturer's instructions. The quality of RNA samples was determined using an Agilent 5400 Bioanalyzer, and all samples for sequencing had RNA integrity (RIN) numbers >8. cDNA library construction using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® was performed by the Novogene Corporation Inc. (Sacramento, CA), and cDNA libraries were sequenced on an Illumina HiSeq 2000 or Illumina Novaseq 6000 instruments.