GSM9668985: NEC14, ALId7, VEH, rep2; Homo sapiens; ATAC-seq
Cells were washed once with 1X PBS and dissociated from cytology brushes prior to counting. Approximately 50K cells were pelleted and processed in duplicate or quadruplicate as described in the Omni-ATAC-seq protocol published by Corces et al (Nature Methods, 2017). Uniquely indexed sequencing adapters from the Nextera DNA CD Indexes Kit were added to both ends of transposed fragment libraries by PCR. Following final purification and size selection for 100-1000 bp fragments, libraries were pooled and sequenced on an Illumina NextSeq instrument using 150 bp paired-end reads.