GSM9128203: Parental 4T1 CD45; Mus musculus; RNA-Seq
Tumors were digested in RPMI with 2 mg/mL collagenase A and 100 U/mL hyaluronidase at 37°C for 2 hours, filtered through 70 μm and 40 μm strainers, treated with RBC lysis buffer, and separated into CD45+ and CD45- fractions using CD45 magnetic beads (Miltenyi). Single cells were fixed using the Chromium Next GEM Fixed RNA Sample Prep Kit (10x Genomics) and libraries were constructed using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 following manufacturer's protocol.