RNA-Seq of thermophilic bioreactor: end of operational Phase I
DNA isolation was performed with the DNeasy PowerSoil Pro Kit (QIAGEN, Germany). PCR amplification of 16S rRNA gene V4 and V5 hypervariable regions was carried out with 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 907R primers (5'-CCGTCAATTCCTTTGAGTTT-3').