GSM8449533: cct1-2 DMSO Rep2; Saccharomyces cerevisiae; RNA-Seq
Biotinylated RNA was collected using glycogen blocked MyOne Streptavidin C1 Dynabeads (Invitrogen). Libraries were constructed as described in Gvozdenov et al. 2023. Briefly, 5' and 3' primers were ligated onto RNA fragments, cDNA was made and amplified with index primers. Total RNA was subjected to polyA selection and fragmentation using Magnesium RNA Fragmentation Module (NEB) before biotinylation.