GSM8594568: P2 Human Colon Cancer.; Homo sapiens; OTHER
5 µm sections were taken from the FFPE tissue blocks with a microtome (Epredia HM355S). Sections were adjacent or near-adjacent (within 5-10 µm of each other). Sectioning followed the Visium CytAssist Spatial Gene Expression for FFPE – Tissue Preparation Guide (CG000518, Rev C). We placed FFPE tissue sections on plain glass slides for deparaffinization, H&E staining and imaging following the Visium HD FFPE Tissue Preparation Handbook (CG000684). Probe hybridization, probe ligation, slide preparation, probe release, extension, library construction, and sequencing followed the Visium HD Spatial Gene Expression Reagent Kits User Guide (CG000685). Sequencing was performed on an Illumina NovaSeq 6000 with paired-end reads (43 cycles Read 1, 10 cycles i7, 10 cycles i5, 50 cycles Read 2).