GSM9017977: AML3_UNT_rep1; Homo sapiens; RNA-Seq
Whole-cell RNA from one million cells of OCI-AML3 cells with or without RA treatment were purified using RNeasy Mini Kit (QIAGEN Cat#74134) Non strand specific library  Messenger RNA was purified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was  synthesized using random hexamer primers followed by the second  strand cDNA synthesis. The library was ready after end repair, A-tailing,  adapter ligation, size selection, amplification, and purification. The  library was checked with Qubit and real-time PCR for quantification and  bioanalyzer for size distribution detection.