RNA-seq of Ixodes scapularis larvae during Borrelia burgdorferi acquisition
Unfed larvae were removed from the incubator and total RNA extraction was performed with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to the manufacturer's specifications.The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.