GSM8968721: SK-N-AS cells of rs1950834-AA, DX 2; Homo sapiens; RNA-Seq
RNA was harvested using RNeasy min plus kit (Qiagen). 2μg taotal RNA was used for sequencing. 1) Perform mRNA enrichment on total RNA using mRNA enrichment method: Use magnetic beads with Oligo dT to enrich mRNA with polyA tails. 2) Fragment the obtained RNA with fragmentation buffer, reverse transcribe using random N6 primers, and then synthesize cDNA double strands to form double-stranded DNA. 3) Fill in the ends of the synthesized double-stranded DNA, 5' end phosphorylate, form a sticky end with an "A" protruding at the 3' end, and then connect a bubble-shaped adapter with a protruding "T" at the 3' end. 4) The connected products undergo PCR amplification using specific primers. 5) Denature the PCR products into single strands, and then use a bridge primer to circularize the single-stranded DNA to obtain a single-stranded circular DNA library.