GSM9196796: trophoblast cells from women with normal pregnancy; Homo sapiens; RNA-Seq
RNA samples from six placental samples from women with normal pregnancies and six placental samples from women with severe PE were separately extracted and pooled into two groups for RNA sequencing. Library preparation and validation quality checks were performed before next-generation sequencing. Ribodepletion was performed using Ribo-Zero H/M/R Gold. Using an Agilent Technologies 2100 Bioanalyzer, the total RNA integrity was checked, and samples with an RNA integrity number equal to or greater than 7 were retained. The template size distribution was checked on an Agilent Technologies 2100 Bioanalyzer using a DNA 1000 chip to verify the size of the PCR-enriched fragments. Transcriptome sequencing was performed with the Illumina platform.