Botrytis-cinerea treated with Spinetoram for 12 hours, with a final concentration of EC90
Mycelial plugs (4 mm diameter) from the edge of 3-day-old colony of the CGMCC3.20932 was transferred to PDB medium culture for two days,with treated with EC90 concentration of Spinetoram , and inoculated in a shake cultivationat 25C for 12h. The RNA of the total sample was isolated and purified by TRIzol (thermofisher, 15596018) according to the operation scheme provided by the manufacturer.  Then the quantity and purity of total RNA was controlled by NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of RNA was detected by Bioanalyzer 2100 (Agilent, CA, USA). The concentration was >50ng/L, RIN value >7.0, and total RNA>4g.