GSM9458403: Convergent hiPSC, uninduced, rep2; Homo sapiens; OTHER
Following the protocol of Goel et al. 2023 (https://doi.org/10.1038/s41588-023-01391-1), in brief, cells were crosslinked with both formaldehyde and DSG. The resulting cross-linked chromatin was fragmented using MNase and ligated after end-repair with biotinylated dNTPs as in Micro-C. Following the protocol above, after selection for the ligated junctions, cross-links were reversed and indexed libraries were prepared following the NEB Ultra II DNA Library Prep kit. Using the Twist Target Enrichment protocol and using an oligo pool targeting the regions listed above, the libraries were region-captured, re-amplified, and sent for sequencing.