GSM9127059: v3_J2_input_scramble_3; Homo sapiens; RNA-Seq
J2-immunoprecipiated RNAs were isolated using the Magna RIP Kit (Sigma) and a phenol-chloroform extraction. Input RNA were isolated using a phenol-chloroform extraction. RNA libraries were prepared using an abundance-based rRNA-depleted total RNA kit (Zymo), a polyadenylated RNA kit (Universal Plus), and a probed-based ribosomal RNA depleted total RNA kit (NEBNext/Zymo). All protocols were followed according to the manufacturers' protocols.