GSM9196761: H9; Mus musculus; RNA-Seq
Kidney tissue was lysed in RLT buffer and homogenized. RNAeasy Micro or Mini kit (Qiagen) was used to extract mRNA according to the manufacturer's protocol. Library preparation and sequencing was performed as described previously (Hagemann-Jensen M, et al. Single-cell RNA counting at allele and isoform resolution using Smart-seq3. Nature biotechnology. 2020;38(6):708-14.).