GSM8785637: Multimodal, T cells, D5. Empty Vec rep 1-3, ADT; Mus musculus; OTHER
Live CD8ab+ T cells were sorted and antibody labeled single cell suspensions were subjected to counting and viability checks before dilution to a concentration of 700-1000 cells/ml. For each treatment group, cells previously labelled with a unique HashTag antibody (N=3 per treatment group) were pooled. Single cell Gene Expression and Total-Seq C libraries were generated using the 10x Genomics Chromium Controller with 5' Gene Expression reagents with Feature Barcoding following the manufacturer's protocol (10x Genomics) before paired-end sequencing (Illumina NovaSeq X Plus).