Smu-3'RR sequencing of mus musculus
S-3RRrec junctions were amplified with primers containing specific linkers to Illumina adapter sequences. Library concentrations were determined by Qubit DsDNA high sensitivity (Invitrogen) and fragment size was assessed through 2100 Bioanalyzer (Agilent). Sequencing libraries were sequenced using 300bp paired-ended sequencing on a MiSeq (Illumina). Forward and reverse reads were merged and were analyzed using CSReport (Boyer et al., Journal of Immunology 2017).