GSM8787485: iPSC-Neuron, Day 21, CROP-seq, rep1; Homo sapiens; RNA-Seq
These cells were dissociated using papain, then pelleted at 300 xg for 5 minutes. These were resuspended and washed in dPBS. The centrifugation and wash steps were repeated for a total of 4 washes to completely remove papain and cell debris. The resuspension was counted and diluted to target 20k cells for a single 10X Chromium GEM-X 3' chip. Cells were prepared according to 10x Genomics general sample preparation protocol. sgRNA enrichment was performed using three semi-nested PCR reactions.