ITS amplicon-seq: 1400m_p2 (2022)
Soil DNA was extracted using a DNeasy PowerSoil Pro Kit and quantified by a Nanodrop spectrophotometer. PCR amplification was performed using the ITS3F and ITS4R primers. The amplified products were purified using QIAquick PCR purification kit and sequencing was performed using the MiSeq platform.