WT H9s (-)SHH 1
mRNA was prepared using the PureLink RNA Mini Kit (ThermoFisher Scientific 12183018A) before handing off samples to Vanderbilt University Medical Center (VUMC) Vanderbilt Technologies for Advanced Genomics (VANTAGE). VANTAGE performed RNA quality control (QC) (with all samples achieving a QC assessment of 98% or higher) before moving towards stranded mRNA (NEB) library preparation and Novaseq X series PE150 sequencing.