RNA-seq of Caenorhabditis elegans
First strand cDNA was synthesize dusing random oligonucleotides and SuperScript II. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activities and the enzymes were removed. After adenylation of the 5 ends of the DNA fragments, Illumina PE adapter oligonucleotides were ligated to prepare for hybridization.