GSM9156069: Brunello Plasmid; Homo sapiens; OTHER
For DNA extraction we used Qiagens QIAamp DNA Blood Maxi Kit (#51194), for each DNA extraction we used pellets from 100Mio cells sgRNA amplification and library construction were performed using a single step PCR with customized primers. For each sample 24 seperate PCRs with 10µg DNA per sample were performed using Agilents Herculase II PCR Kit. PCR products were size selected using AMPure beads and a TapeStation (Agilent) was used for Quality control.