WGS of Malurus melanocephalus
Genomic DNA was extracted from each sample using DNeasy blood and tissue extraction kits (Qiagen, California, USA) and DNA concentrations were determined using a Qubit fluorometer (Life Technologies, California, USA). We used 200 ng of DNA from each sample to prepare individually barcoded libraries with a 550 bp insert size following the protocol for the TruSeq Nano DNA Library Prep kit (Illumina, California, USA). The libraries of the 20 plumage transition zone birds were pooled and then sequenced together on two lanes of on an Illumina NextSeq500 (2  150 bp) at the Cornell University Biotechnology Resource Center. The remaining 12 libraries of the two allopatric population birds were sequenced on a third lane of an Illumina NextSeq500 lane (2  150 bp).