RNA-seq from the gill of control 1
After a 27-day experimental period, mussels were assessed for reproductive stage, as before, and determined to no longer be carrying glochidia. Mussels were then euthanized to collect tissue samples for gene expression analysis. For each mussel, three tissues were sampled (foot, mantle, gill) and preserved in RNA later, stored at 4 C for 24 h and after stored at -80 C (see: Differential gene expression (DGE) analyses).