Microglia isolated from injured but untreated mice (1 month post-TBI), following the adoptive transfer of Total CD4+ Tregs from injured mice treated with nasal anti-CD3 (aCD3), Biological Replicate #3
total splenic CD4+ T cells from isotype and aCD3-treated donor mice (CD45.2) and CD4+ FoxP3 GFP negative population from aCD3-treated donor mice (CD45.2) at 7 days post-TBI were intraperitoneally transferred into untreated but TBI-injured recipient mice (CD45.1). Bulk RNA-sequencing analysis was performed on microglia isolated from the ipsilateral hemisphere of injured but untreated mice at 1 month post-TBI following adoptive transfer of total CD4+ T cells from TBI-Iso mice, total CD4+ T cells from TBI-aCD3 mice, and CD4+ T cells depleted of FoxP3+ cells from TBI-aCD3 mice.