WGS of rhizospheric soil metagenome: 2a
The PCR amplicons were bead purified using AMPure XP magnetic beads to remove PCR reagents and other contaminant and subsequently used for library preparation involving end prep, barcode ligation (EXP-NBD104 & EXP-NBD114) and adaptor ligation, as per the protocol (Native barcoding of Amplicons) prescribed from Oxford Nanopore Technologies (ONT). In the final step, the library was processed using SQL-LSK 109 sequencing kit and 300- 350 ng library was loaded onto the MinION Mk1B sequencer. Sequencing was carried out to generate ~1,50,000 reads per sample including cumulative reads of Bacteria, Archaea and Fungi.