Microglia isolated from injured but untreated mice (7 days post-TBI), following the adoptive transfer of FoxP3+ Tregs from injured mice treated with nasal isotype (Iso), Biological Replicate #1
splenic/cervical lymph nodes CD4+ FoxP3+ Tregs from isotype and anti-CD3 treated donor mice (CD45.2) and CD4+ FoxP3(GFP) negative population from anti-CD3 treated donor mice (CD45.2) at 7 days post-TBI were intraperitoneally transferred into injured but untreated recipient mice (CD45.1). Bulk RNA-sequencing analysis was performed on microglia isolated from the ipsilateral hemisphere of injured but untreated mice at 7 days post-TBI following adoptive transfer of CD4+Foxp3+ Tregs from TBI-Iso mice, CD4+Foxp3+ Tregs from TBI-aCD3 mice, and CD4+ T cells depleted of Tregs from TBI-aCD3 mice.