RNA-seq of Morella Rubra replicate 3
Using an RNA Easy Kit (Qiagen), the total RNAs of fruit were extracted, immediately frozen in liquid nitrogen, and stored at 80C until use. According to the instruction of the manufacturer, after purification and DNA digestion with RNase-free DNase (Qiagen), the RNA samples (5 g per sample) were analyzed using an Agilent 2100 Bioanalyzer (28S/18S =1.8-2.0) and a NanoDrop ND-2000 (A 260/A 280 =1.9-2.1). The mRNA Capture Beads were thoroughly mixed on a 4-D rotator and equilibrated for 30 min before use.