GSM9456973: adenoma tissue, control conditions; Mus musculus; OTHER
Tissue was processed as described in the Visium HD protocol with the following modifications: the OCT-embedded swiss rolls were sectioned on a cryostat at 10 μm thickness and mounted on a Fisherbrand™ Superfrost™ Plus glass slide. The slides were fixed using 4% PFA and stained using H&E method. During that process the wash buffers were supplemented with either ribonucleoside vanadyl complex or a commercial RNAse inhibitor. After imaging of the H&E staining, the samples were destained and permeabilized using 1% SDS and then pre-chilled 70% methanol. Libraries were prepared per Visium HD protocol.