Pupal wings (fore- and hind-wings), 40% stage, female, short RNA sequencing
Tissues were stored in TRI-reagent, Total RNA was isolated, and submitted to Azenta NGS (Genewiz) for miRNA purification using the Qiagen miRNeasy Kit, library preparation, and sequencing at a target depth of 10M reads. Small RNA-seq libraries (miRNA, siRNA, piRNA) were prepared by size fractionation with adapter ligation to 5 phosphate