GSM8853967: ControlKD_CandR_SRCAP; Mus musculus; OTHER
After treatment, nuclei were extracted as outlined in CUT&RUN. Nuclei were bound to ConcanavalinA beads, washed, followed by successive addition of primary antibodie followed by proteinA-Mnase. Cleavage of Mnase was initiated by Ca** addition and stopped by chelation. Fragments were released and deep sequencing libraries were prepared.