GSM8965482: 2CRD_1726; Danio rerio; RNA-Seq
RNA extraction was carried out using the Qiagen micro RNeasy kit and manufacturer's instructions were followed with subsequent Single Cell RT-PCR method for cDNA synthesis and subsequent PCR amplification developed in: Zhou T, Chien MS, Kaleem S, Matsunami H. 2016. Single cell transcriptome analysis of mouse carotid body glomus cells. J Physiol 594:4225-51. Illumina Sequencing Library Preparation method developed in: Zhou T, Chien MS, Kaleem S, Matsunami H. 2016. Single cell transcriptome analysis of mouse carotid body glomus cells. J Physiol 594:4225-51., was used whereby the amplified cDNA was purified with magnetic beads (Agencourt AMPure XP) and 50ng of DNA was tagmentated using the Nextera DNA sample preparation kits (Illumina Inc). Sequencing was carried out by the Duke Sequencing and Genomic Technologies Shared Resource