GSM8942617: pbmc,HC3; Homo sapiens; RNA-Seq
Total RNA was extracted from PBMCs using Trizol reagent (Vazyme). RNA quality control was performed using NanoPhotometer and Agilent 2100 RNA Nano 6000 Assay Kit (AgilentTechnologies, CA, USA). Each sample was prepared using a total of 5 μg RNA. Libraries were generated by TruSeq® RNA Sample Prep Kit (Illumina, USA) following manufacturer's recommendations.