GSM8387227: Dhx16_fl3; Mus musculus; RNA-Seq
About 5000 HSC (Lin-Sca-1+c-Kit+CD48-CD150+) were isolated by FACS and the mRNA library constructed using Smart-Seq V4 Ultra Low Input RNA Kit for sequencing (Takara) according to the manufacturer's instruction RNA libraries were prepared for sequencing using standard Illumina protocols The mRNA library constructed using Smart-Seq V4 Ultra Low Input RNA Kit for sequencing (Takara) according to the manufacturer's instruction.RNA libraries were prepared for sequencing using standard Illumina protocols