MiSeq rhesus macaque: eye microbiome
DNA extractions were performed using the Macherey-Nagel DNeasy-96-PowerSoil Pro kit. Samples were processed into NGS libraries using Illumina 16S Metagenomic Sequencing Library Preparation using an adjusted low biomass protocol. 11.5ul of stock sample was used as input into the first stage PCR. Adjustments to the library prep protocol to account for the low DNA input included: a reduction in PCR bead clean up elution volumes to 30ul for PCR clean up 1 and to 20ul for PCR clean up 2; adjustments to the indexing reaction include 3x volume increase in amplicon used along with a removal of PCR grade water. Libraries were sequenced on two MiSeq 600 cycle v3 sequencing runs, PE300 with 25% phiX spike in due to the low diversity nature of the libraries.