GSM8594503: ATAC-seq_THP-1_SA_rep2; Homo sapiens; ATAC-seq
THP-1 cells were seeded at a density of 5×105 cells ml-1 in 6-well plates and exposing the cells to 200 ng ml-1 PMA) for 24 h. After SA, HS and control treatment, cells were centrifuged for 10 min at 1,000 g (4 °C) using a swinging-bucket rotor, and the supernatant was carefully removed to avoid disturbing the cell pellet. The cells were lysed for 5 min in 50 μl of cold resuspension buffer (RS buffer, 0.1% NP-40, 0.1% Tween-20, 0.01% digitonin). The nuclei were pelleted for 10 min at 1,000g (4 °C). The supernatant was carefully removed resuspended in 50 μl of transposition mix (16.5 μl TW buffer, 4 μl TTE Mix V50, 10 μl 5×TTBL, 0.5 μl of 1% digitonin, 0.5 μl of 10% Tween-20 and 18.5 μl double-distilled water) and incubated for 30 min at 37 °C on a thermomixer. The DNA was then purified using ATAC DNA Extract Beads according to the manufacturer's instructions and eluted in 21 μl of elution buffer. ATAC-seq was performed with Hyperactive ATAC-seq Library Prep Kit (Vazyme).The DNA was then purified using ATAC DNA Extract Beads according to the manufacturer's instructions and eluted in 21 μl of elution buffer. Libraries were amplified as originally described and the final preparations were purified using ATAC DNA Clean Beads.