GSM8594252: DOHH2_AAVS_BSL_1; Homo sapiens; RNA-Seq
Total RNA was purified using the Trizol reagent followed by RNAeasy column purification (Qiagen). RNA was assessed using the Bioanalyzer (Agilent #5067-1513) and a Qubit RNA High Sensitivity assay (Thermofisher # Q32855), and 50ng of RNA input was processed The libraries were barcoded, pooled, and sequenced using paired-end, 151 bp sequencing at the University of Michigan Advanced Genomics Core.