Metagenome Seq of canine feces: zr3336_50
Total genomic DNA was extracted from the individual fecal samples using QI-Aamp DNA Stool Mini Kit (Qiagen) following the manufacturers instructions. The individual DNA samples were sequenced and analyzed using ZymoBIOMICS shotgun sequencing pipeline (Zymo Research, Irvine, CA), and ZymoBIOMICS bioinformatics analysis pipeline. Briefly, genomic DNA samples were profiled with shotgun meta-genomic sequencing. Sequencing libraries were prepared with the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA) with up to 100 ng DNA input following the manufacturer's protocol using internal dual-index 8 bp barcodes with Nextera adapters (Illumina, San Diego, CA). The ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA) was used as a positive control for each targeted library preparation. Negative controls (i.e., blank library preparation control) were in-cluded to assess the level of bioburden carried by the wet lab process. All libraries were quantified with TapeStation (Agilent Technologies, Santa Clara, CA) and then pooled in equal abundance. The final pool was quantified using qPCR. The final library was sequenced (paired-end, 2 150 bp) on the Illumina NovaSeq.