metagenomic analysis of scalp bacterial microbiota of 4 volunteer after treatment
Post-treatment sample from a 4*4 cm area on the hairy part of the scalp after 3-hour incubation with 1 mL of the enzyme solution (0,25% chitinase (100 U/g) and 0,25% chitosanase (200 U/g)). DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, USA) according to the manufacturers protocol. Metagenomic libraries were prepared following the Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System protocol. The V3V4 region of the 16S rRNA gene was amplified with the following primers: forward (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3) and reverse (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC-3). Amplicons were indexed and sequenced on an Illumina MiSeq system (Illumina, USA) in 2  300 bp paired-end mode.