GSM8921790: Vehicle liver metastasis Epcam+/CD45- sorted epithelial cells; Mus musculus; RNA-Seq
FACS-sorted Epcam+/CD45- epithelial cells were concentrated by centrifugation (500g for 10 min at 4ºC) and counted. Cells were partitioned into Gel Bead In Emulsions (GEMs) with a target of 10.000 cells per sample using the Chromium Controller system (10X Genomics). cDNA sequencing libraries were prepared using the Chromium Single Cell 3ʹ Kit v3 (PN-1000075). cDNA was amplified, quality checked, and quantified on an Agilent Bioanalyzer High Sensitivity chip (Agilent Technologies), then used for Gene Expression (GEX) library preparation (PN-220103 Chromium i7 Sample Index Plate). Final libraries were indexed by PCR and verified for size and concentration. Sequencing was performed on an Illumina NovaSeq 6000 to obtain approximately 40.000 reads per cell.