GSM8650303: U-87MG_CD47-CRISPR-Knockout_rep1; Homo sapiens; RNA-Seq
Total RNA was extracted using TRIzol (Thermo Fisher Scientific, 15596018) according to the manufacturer's protocol. RNA integrity was established with Bioanalyzer 2100 (Agilent) trace. 1 μg of total RNA was then subjected to NEB polyA selection with oligo dT beads (New England Biolabs, E7490). Enriched polyA RNA was utilized for library preparation using the KAPA RNA Hyperprep kit (Roche, 08098107702) following manufacturer's guidelines.