Transcriptomic analysis of monosomy X (45,X) tissues during early human fetal and placental development
Transcriptomic analysis of monosomy X (45,X) tissues during early human fetal and placental development
Human fetal tissue samples used for bulk-RNA seq were obtained with ethical approval and informed consent from the Human Developmental Biology Resource (http://www.hdbr.org).The age was estimated from foot length and knee-heel length in relation to standard growth data. Samples were karyotyped by quantitative PCR (chromosomes 13, 15, 16, 18, 21, 22 and X and Y) to determine the sex of the fetus. Samples were stored in -70C until use. Total RNA was extracted from human fetal tissue samples using the AllPrep DNA/RNA Mini Kit (Qiagen). cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche) on a Hamilton Starlet robotic platform (Hamilton) and quality was analyzed using a Tapestation 4200 platform (Agilent technologies).