GSM8384958: BAT_DTR-_D4#3; Mus musculus; RNA-Seq
For RNA.seq on Tregs: The cells were double-sorted directly into 5 μl TCL (Qiagen) buffer containing 1% 2-mercaptoenthonol (Sigma). The cells were sorted as DAPI-CD45+Tcrb+CD4+Thy1.1+ from fat tissues of Foxp3.Th1.1 reporter mice at 8 weeks.  For whole tissue experimentes 2 ng RNA was added to 5 μl TCL (Qiagen) buffer containing 1% 2-mercaptoenthonol (Sigma). Total RNA was purified using RNAClean XP beads (Beckman Coulter). Polyadenylated mRNA was selected using an anchored oligo(dT) primer (5′–AAGCAGTGGTATCAACGCAGAGTACT30VN-3) and then reverse transcribed to cDNA. First-strand cDNA was subjected to limited PCR amplification followed by Tn5 transposon-based fragmentation using the Nextera XT DNA Library Preparation Kit (Illumina). Libraries were constructed following the Smart-seq2 RNA-seq library preparation protocol.