GSM9155821: CK1i_2125; Mus musculus; RNA-Seq
The splenic leukemic B cells from AT recipient mice were sorted out of the bulk splenocytes as described above. Cells were further spun, lysed and processed for RNA isolation using ReliaPrep Cell Miniprep System (Promega, #Z6011) according to manufacturer's instructions. The library preparation was done using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen, #015.96)    in combination with UMI Second Strand Synthesis Module for QuantSeq FWD (Lexogen, #081.96) in accordance with the manufacturer's instructions. The sequencing was performed at the Illumina NextSeq500 sequencer with read length 1x75 bp.