GSM8785858: HSMM myofiber sheet, condition No4-1; Homo sapiens; RNA-Seq
Myofiber sheets were incubated in an enzymatic digestion solution containing 2% v/v dispase (#354235, Corning) and 15 mg/mL collagenase type II (#LS0041174, Worthington-Biochem) for 3-5 min to detach cells from the gel. The cell suspension was immediately centrifuged to collect the detached cells. RNA was then isolated using the RNeasy Plus Mini Kit (#74104, Qiagen) according to the manufacturer's protocol. Poly(A) RNA preparation was conducted using Poly(A) mRNA magnetic isolation module (New england Biolabs, Ipswich, MA, USA) following the manufacturer's protocol. Library construction was conducted using NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA) following the manufacturer's protocol.