Rep-Seq of zebrafish TU IgM without immunization: adult kidney fish4
A method was designed for constructing a TU strain zebrafish B-cell receptor (BCR) library based on the SMARTer Human BCR IgG IgM HKL Profiling Kit. In the 4 individuals without immunization, a single TU zebrafish kidney was utilized to construct a library of fish4 IgM.