Gamma/delta TCR VDJ library from hashed LHES samples
Four consented LHES samples (P4_LHES; P3_LHES_PRE; P3_LHES_POST; P5_iHES) were hashed using BioLegend TotalSeq-C human hashtags (C0251–C0255) and pooled into a single 10x Genomics Chromium 5′ v2 library. A single cell capture produced separate libraries: gene expression (GEX), alpha/beta (a/b) VDJ, gamma/delta (g/d) VDJ and antibody-derived tag (ADT). Libraries were sequenced on an Illumina NovaSeq 6000 and processed with Cell Ranger 6.1.0 against GRCh38. Demultiplexing was performed using Seurat HTODemux. HTO mapping: P3_LHES_PRE→HTO3 (TTCCGCCTCTCTTTG), P3_LHES_POST→HTO5 (AAGTATCGTTTCGCA), P4_LHES→HTO2 (TGATGGCCTATTGGG), P5_iHES→HTO4 (AGTAAGTTCAGCGTA). See FSM_library and FSM README for details.