Hi-C sequencing of Thinopyrum intermedium for genome assembly
Fresh leaf tissues from Thinopyrum intermedium were fixed in 2% formaldehyde solution prepared in MS buffer. Nuclei were isolated by homogenizing the tissue in liquid nitrogen, followed by filtration through a cell strainer. The cross-linked DNA was subjection to digestion using the restriction enzyme DpnII at 37C. Biotin-labeled adapters were ligated to the sticky ends of the digested fragments to form chimeric junctions. These fragments were enriched and trimmed to approximately 450 bp. Quality-controlled Hi-C libraries were sequenced on the DNBSEQ-T7 platform.