16S v3v4 seq of mus musculus vaginal swab
Zymo Quick-16S Plus NGS Library Prep Kit (#D6421). V3/V4 amplicon. Primers used: 341f (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG, 17 bp) and 806r (GACTACNVGGGTMTCTAATCC, 24 bp). The forward primer 341f is a mixture of the two sequences listed. Sequencing was performed on the Illumina NextSeq2000 platform using a 600 cycle flow cell kit to produce 2x300bp paired reads. 30-40% PhiX control (unindexed) was spiked into the library pool to support optimal base calling of low diversity libraries on patterned flow cells.