RNA-seq of HepG2 cells (HepG2_PL)
Total RNA was extracted from HepG2 cells using Trizol reagent (Invitrogen) or Maxwell RSC miRNA Kit (Promega). RNA quality was checked with Agilent 4200 TapeStation and quantified using Qubit (Thermo Fisher). Libraries were prepared using QuantSeq 3 mRNA-Seq Library Prep Kit FWD (Lexogen) according to the manufacturers instructions. Sequencing was performed as single-end 75 bp on an Illumina NextSeq 500/550 platform. Downstream analysis included QC (FastQC), trimming (bbduk), alignment (STAR), quantification (HTSeq-count), and normalization (Conorm).