GSM9230035: Lung, isoLCA, rep2; Mus musculus; RNA-Seq
Obtained lung homogenates were first filtered through 70 µm and subsequently 40 µm cell strainers, and centrifuged for 10 min, 300 xg at 4˚C. Erythrocytes were lysed with RBC lysis solution for 15 min at 4˚C. Cells were then prepared according to ScaleBioTM fixation protocol. ScaleBio™ Single Cell RNA Sequencing Kit v1.1