GSM8919956: 28hpf_sox17N_RNA_rep2; Danio rerio; RNA-Seq
Cell populations were isolated according to fluorescence profiles using a BD FACSAria Fusion flow cytometer.  For RNA-seq cells were pelleted and total RNA extracted using QIAGEN Rneasy Mini kits. RNA-seq libraries were produced from 10 ng total RNA each using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina according to the manufacturer's instructions and were sequenced using Novaseq 6000-S4-type flow cell.