GSM8385874: PM_1,scRNAseq; Homo sapiens; RNA-Seq
The cell suspension was loaded into Chromium microfluidic chips with 3'(v2 or v3depends on project) chemistry and barcoded with a 10x Chromium Controller (10XGenomics). RNA from the barcoded cells was subsequently reverse-transcribed Sequencing libraries constructed with reagents from a Chromium Single Cell 3' v2(v2 or v3, depends on project) reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (HiSeq 2000 or NovaSeq ,depends on project) according to the manufacturer's instructions (Illumina)