GSM8539475: A549 cells, deep_sequencing, shD3#1, biol_rep_1; Homo sapiens; RNA-Seq
RNeasy Mini Kit (QIAGEN, 74104) was used for RNA extraction. An additional step of DNase digestion was incorporated after the first wash following the RNase-Free DNase Set protocol (QIAGEN, 79254). 13 µl of rRNA depleted RNA sample were used for the preparation of the RNA-seq library. The NEXTFLEX® Rapid Directional RNA- Seq Kit 2.0 protocol was followed to perform all the remaining steps for library construction steps.