scATAC of mus musculus: fancm wildtype sperm
Each single-gamete ATAC-sequencing library, haploid sperm nuclei were FACS sorted into a 5 mL polypropylene tube containing 300 L NWRB with 0.1% (w/v) BSA. Nuclei were centrifuged at 500  g for 5 minutes at 4oC and the supernatant was aspirated. Nuclei were resuspended in NWRB with 0.1% (w/v) BSA supplemented with 0.01% (v/v) Digitonin and incubated on ice for 5 minutes. Nuclei were centrifuged again at 500  g for 5 minutes at 4oC and the supernatant was aspirated. 500 L of Diluted Nuclei Buffer (10x Genomics) was slowly added to the nuclei without disrupting the pellet and allowed to buffer exchange for 5 minutes on ice. The nuclei were resuspended in 10 L of Diluted Nuclei Buffer (10x Genomics) and quantified using a hemocytometer. Nuclei concentration was adjusted to attain a capture number of around 1,000 nuclei, accounting for loss during subsequent steps. Prepared nuclei were added to the Transposition Mix (10x Genomics) and transposition, library preparation and clean-up for single-cell ATAC-sequencing was performed according to the manufacturers protocol using the Chromium Single-Cell ATAC Kit v1.1 (10x Genomics). Each library was sequenced on the NovaSeq 6000 (Illumina) using a 50/8/16/50 bp read configuration. Tagmentation was used for library selection.