Pig_16S
Total bacterial DNA was extracted from faecal samples  using the FastDNA SPIN Kit for Soil (DBA Italia, Segrate, Italy) following the manufacturer's instructions. The library formation and sequencing of the 16S rRNA gene were carried out using the MiSeq Reagent Kit V3-V4 (Illumina Netherlands, Eindhoven the Netherlands) on a MiSeq-Illumina platform.