GSM8751245: sample.MM.1S_E9_GFP_2; Homo sapiens; RNA-Seq
From the sorted populations, three biological replicates were prepared for each cell line, MM.1S WT and KO respectively, snap frozen in liquid nitrogen, and stored at –80°C. RNA extraction was carried out at the Tissue Procurement Core of Washington University using the QIAamp RNA Mini Kit (Qiagen). Total RNA integrity number (RIN) was determined using Agilent Bioanalyzer or 4200 Tapestation. Library preparation was performed with 10ng of total RNA with a Bioanalyzer RIN score greater than 8.0. ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol.