K2
Pancreatic tissues (20 to 25 mg) were collected in ice-cold RNAlater stabilization solution (AM7020, Thermo Fisher Scientific) and subjected to RNA extraction within 4 hours. Total RNA was extracted using Trizol reagent (15596018CN, Invitrogen). RNA quantity was measured by the NanoDrop ND-100 (Thermo Fisher Scientific) and RNA integrity was checked by Bioanalyzer 2200 (Agilent Technologies). cDNA library construction and RNA sequencing were performed on an Illumina HiSeqTM 2500 platform at OE Biotech Company (Shanghai, China).