GSM8925991: K5:Smo organoids treated with TGFb inhibitor (CTRL) - 3 days rep1; Mus musculus; RNA-Seq
Organoids were harvested and collected in TRK lysis buffer. Total RNA was extracted using E.Z.N.A Total RNA Kit. RNA quality was checked before library preparation using a Bioanalyzer 2100. Libraries were obtained using the Ovation Solo RNA-seq System (NuGen) following manufacturer's recommendations and loaded on a Novaseq 6000 (Illumina)