Single-Cell V(D)J sequencing of mice
Single-cell suspensions were loaded to 10x Chromium to capture 8000 single cell according to the manufacturers instructions of 10X Genomics Chromium Single-Cell 3 kit (V3) .The following cDNA amplification and library construction steps were performed according to the standard protocol. Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run,150bp) by LC-Bio Technology co.ltd., (HangZhou,China) at a minimum depth of 20,000 reads per cell.