GSM8853466: 10daysChronicRestraint_3h_CRS3_v21; Mus musculus; RNA-Seq
One snap-frozen ventral hippocampus per animal was homogenized in 500 µl Trizol (Invitrogen 15596018) using metal beads (Qiagen 69989) in a tissue lyser bead mill (Qiagen, Germany) at 4°C for 2 min at 20 Hz. RNA was extracted according to the manufacturer's recommendations. The CRS1 experiment was prepared using the Illumina TruSeq stranded RNA kit, while the other libraries were prepared at Novogene using the NEBNext® Ultra RNA Library Prep Kit.