GSM9202333: E16.5 embryonic BAT, replicate 3; Mus musculus; RNA-Seq
Tissues were lysed in TRIzol (Invitrogen #15596018) using a TissueLyser II (QIAGEN) for 4 minutes at 24 Hz in pre-chilled tube adapters. Total RNA of BAT from E15.5-E18.5 embryos and P0.5 neonates was isolated using TRIzol and purified using PureLink RNA Mini columns (Invitrogen #12183025). Library preparation with poly(A) selection and Illumina sequencing were completed by Azenta/GENEWIZ.