PI4KA6
PTECs were isolated from Pi4kawt/wtKspCre-ERT2 and Pi4kafl/flKspCre-ERT2 mice. Total RNA was extracted using TRIzol reagent (under RNase-free conditions) according to the manufacturer's protocol. Briefly, cells were rinsed with PBS, gently detached by pipetting, and pelleted by centrifugation at 500  g for 5 minutes at 4C. Each sample containing up to 10 cells was lysed in 1mL TRIzol, and total RNA was purified via phenolchloroform extraction. RNA quality and integrity were assessed with NanoDrop and Agilent Bioanalyzer. RNA sequencing and library construction were performed by Tsingke Biotechnology Co., Ltd. (Beijing, China) using poly(A) selection and paired-end sequencing on an Illumina platform.