RNASeq of Homo sapiens sample KLE Ctrl 1
RNA was extracted from cultured cells with Qiagen's RNAeasy Plus kit, ribosomally depleted using Takara's Ribogone Mammalian kit. cDNA was made using Clontech's SMARTer Universal Low Input RNA Kit for Sequencing, and barcodes were added using the Ion Plus Fragment Library Kit. Two individually barcoded samples were sequenced together on an Ion Torrent S5XL by the Analytical and Translational Genomics Shared Resource at UNMCCC.