WGS of rhizospheric soil metagenome: 12a
The PCR amplicons were bead purified using AMPure XP magnetic beads to remove PCR reagents and other contaminant and subsequently used for library preparation involving end prep, barcode ligation (EXP-NBD104 & EXP-NBD114) and adaptor ligation, as per the protocol (Native barcoding of Amplicons) prescribed from Oxford Nanopore Technologies (ONT). In the final step, the library was processed using SQL-LSK 109 sequencing kit and 300- 350 ng library was loaded onto the MinION Mk1B sequencer. Sequencing was carried out to generate ~1,50,000 reads per sample including cumulative reads of Bacteria, Archaea and Fungi.