PRJEB98918-FCs09-16S
DNA extraction was performed using the DNeasy PowerSoil Pro kit (QIAGEN) following the manufacturer's protocol. DNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer. PCR amplification of the V3-V4 hypervariable region of the 16S rRNA gene was performed using the 341F-785R primer pair, followed by PCR product purification.