GSM8503671: qs864, 090mpi, wt433; Dictyostelium discoideum; RNA-Seq
All cells were collected into RNAlater (Qiagen). Subsequent total RNA extractions from each sample were performed with RNeasy plus Mini kits (Qiagen). Illumina stranded mRNA-seq libraries were prepared and sequenced as 151 bp paired-end reads at University of Minnesota Genome Center (UMGC). Libraries were barcoded, pooled, and sequenced across three lanes to minimize lane (batch) effects.