16s rRNA-seq of mus musculus: gut
PCR amplification of V3-V4 region was performed with primers 341F and 806R connecting with barcodes. The PCR products with proper size were selected by 2% agarose gel electrophoresis. Same amount of PCR products from each sample was pooled, end-repaired, A-tailed and further ligated with Illumina adapters. Libraries were sequenced on a paired-end Illumina platform to generate 250bp paired-end raw reads. Barcodes and primers are still present in the submitted raw reads. Samples were demultiplexed prior to submission by the sequencing facility.