Male mouse dorsal hippocampus, injected with Acetate, collected 30m after injection from home cage, replicate M4
Total RNA was extracted using TRIzol-chloroform and the RNeasy Mini Kit (Qiagen, Cat. No 74104) including RNase-free DNase (Qiagen, Cat. No. 79254) treatment according to manufacturer protocols. Total RNA quality was assessed using High Sensitivity RNA ScreenTape Analysis on the TapeStation system (Agilent, Cat. No 5067-5579 and 5067-5580). mRNA was isolated from up to 150 ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Cat. No. E7490), and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Cat. No. E7770) in coordination with compatible NEBNext Oligos for Illumina (New England Biolabs, Cat. No. E7335 and E7500).