16s of environment: small pool
DNA was extracted from the swabs using a DNeasy PowerSoil HTP 96 kit (Qiagen) with the bead-beating step consisting of 90 seconds on a Biospec 96 machine. All swabs were tested in duplicate. We used a one-step PCR library preparation and dual-indexed paired-end sequencing to sequence the microbiome of each salamander skin swab sample and all controls. The V3V5 region of the 16S rRNA gene (380 bp) was amplified from samples and controls (field, extraction, and PCR controls) using the universal primers 515F-Y and 939R and sequenced the library on one MiSeq (Illumina) run (2 x 300 V3 kit)