GSM9196428: RNA-seq of Acinetobacter baumannii A1-1; Acinetobacter baumannii; RNA-Seq
Prokaryotic rRNA depletion to enrich mRNA; fragmentation buffer to shear RNA; first-strand cDNA synthesis with random hexamers; second-strand cDNA synthesis with DNA polymerase I and RNase H; AMPure XP purification; end repair, A-tailing, adapter ligation; size selection with AMPure XP; PCR amplification; library quantification by Qubit and qPCR; insert size checked by Agilent 2100 Bioanalyzer. Standard Illumina RNA-seq library construction following vendor protocols as described: rRNA removal (prokaryote), fragmentation, cDNA synthesis, end repair, A-tailing, adapter ligation, PCR amplification, and AMPure XP-based size selection.