Hi-C-Seq of Callicarpa nudiflora: young leaf
Freshly harvested leaves were cut into 2 cm pieces and vacuum-infiltrated with nuclei isolation buffer containing 2% formaldehyde. Glycine was added to stop crosslinking, followed by further vacuum infiltration. The fixed tissue was ground into powder and suspended in nuclei isolation buffer to isolate nuclei. These nuclei were digested with DpnII and labeled with biotin-14-dCTP. Non-ligated DNA ends were cleaned up using T4 DNA polymerase. The ligated DNA was fragmented into 300-600 bp pieces, repaired, A-tailed, and purified using biotin-streptavidin pull-down. Finally, the Hi-C libraries were quantified and sequenced on the MGI-2000 platform.