RNA-Seq of OA G20: Biofilm PC_2
Library preparation and RNA sequencing were carried out at the Oklahoma Medical Research Foundation NGS Core Facility (Oklahoma City, OK, USA). The process involved several key steps: initially, ribosomal RNA (rRNA) was removed using the RiboCop rRNA Depletion Kit (Lexogen, Greenland, NH, USA). Following this, cDNA libraries were constructed using the Swift Rapid RNA Library Kit, adhering to the manufacturer's instructions (Swift Biosciences, Ann Arbor, MI, USA). The quality of the prepared libraries was assessed through Kapa qPCR and an Agilent Tapestation 4150 system (Agilent Technologies, Santa Clara, CA, USA). Sequencing was conducted on the Illumina NovaSeq platform, utilizing an S4 flow cell with a 150-base pair paired-end configuration. This setup generated approximately 20 million reads per sample, with 10 million reads per direction. All experiments were performed in 4 biological replicates to ensure statistical reliability