Amplicon Sequencing of Forest Litter: Unburnt Top Litter
After extracting total DNA from the samples, specific primers containing barcode sequences were synthesized based on the full-length primer sequences. PCR amplification was performed, and the resulting products were purified, quantified, and normalized to construct sequencing libraries (SMRT Bell libraries). The constructed libraries first underwent quality control inspection. Libraries that passed quality control were sequenced using the PacBio Sequel II platform. The raw data output from PacBio Sequel II is in BAM format. CCS (Circular Consensus Sequence) files were derived using the SMRT Link analysis software. Data from different samples were identified according to their barcode sequences and converted into FASTQ format data.