GSM9156994: 4dpi_ATAC; Mus musculus; ATAC-seq
Small intestines were removed from mice and flushed with ice-cold PBS. Intestines were cut into 2mm pieces and washed with ice-cold PBS, and incuabted in 2mM EDTA solution for 40 minutes at 4℃ while on a shaker. Following whole intestinal fragments were shaken vigoursouly in ice-cold PBS to isolate epithelial segments. Solution was then enriched for crypts by passing liquid through a 70 µm strainer. 10X genomics Single Cell Multiome (ATAC + Gene Expression) 10X genomics Multiome ATAC + Gene Expression kit was used and manufactures instructions were followed