ATAC-seq of C57BL/6J, Castaneus, and Caroli mouse livers and C57BL/6J liver tumours
ATAC-seq of C57BL/6J, Castaneus, and Caroli mouse livers and C57BL/6J liver tumours
Livers from mice were isolated and flash frozen at -80°C. A subgroup of C57BL/6J mice were treated with a single intraperitoneal dose of diethylnitrosamine (20 mg/kg) on post-natal day 15 (P15) to induce liver tumours, which were isolated on day P252. Background liver tissue was also collected.  Samples were treated as previously described in Omni-ATAC protocol (Corces et al. 2017), with minor modifications to the nuclear isolation steps (in Step 1, 1 ml of 1× homogeniser buffer was used instead of 2 ml; in Step 4, douncing was performed with 30 strokes instead of 20). ATAC-seq was performed as described previously (Corces et al. 2017).