GSM9371031: UM-SCC-4 shNSD2; Homo sapiens; RNA-Seq
Total RNA was extracted from each samples with the Quick-RNA MiniPrep kit (Zymo Research ##D4020). RNA quality and integrity were assessed with an Agilent 2100 bioanalyzer using an RNA 6000 Nano Kit (Agilent Technologies) mRNA-Seq library was prepared from 500 ng of total RNA with the TruSeq Stranded Total RNA (Illumina)