GSM8889073: SK-N-BE(2)c, ASO10_ATAC, rep3; Homo sapiens; ATAC-seq
25 0000 SK-N-BE(2)c cells were seeded, and next day transfected with ASO2, ASO10, or NCA. Forty-eight hours post transfection cells washed once with PBS, pelleted, and genomic DNA was extracted from 90% of the pellet. Genomic DNA was tagmented by Tn5 transposase and DNA libraries were generated as described previously by Roels et al. 2020 (Nat Immunol, 21, 1280-1292). Libraries were quantified by RT-qPCR with the KAPA Library Quantification kit and were pooled equimolarly. 1.9 pM of the final pool and 1% PhiX were loaded, and paired-end sequencing was performed on a NextSeq 500 instrument (NextSeq 500/550 High-Output kit, 150 cycles) with a 150 bp read length.