DSE-lung5-rnaseq
Poly(A)+ mRNA was purified from total RNA using the Dynabeads mRNA Purification Kit (Invitrogen, USA). Sequencing libraries were prepared with the MGIEasy RNA Library Prep Kit V3.1 (MGI, China) according to the manufacturers protocol, and purified using MGIEasy DNA Clean Beads (MGI, China). The qualified libraries were subsequently subjected to paired-end sequencing on the DNBSEQ-T7 platform (MGI, China).