GSM8594561: P2 Human Colon Cancer; Homo sapiens; OTHER
5 µm sections were taken from the FFPE tissue blocks with a microtome (Epredia HM355S). Sections were adjacent or near-adjacent (within 5-10 µm of each other). Sectioning followed the Visium CytAssist Spatial Gene Expression for FFPE – Tissue Preparation Guide (CG000518, Rev C). 5 µm FFPE serial sections were placed on standard glass slides and H&E-stained following the Demonstrated Protocol Visium CytAssist Spatial Gene Expression for FFPE – Deparaffinization, H&E Staining, Imaging & Decrosslinking (CG000520). Imaging was performed on the slide scanner Olympus V200 at 20x magnification. The CytAssist instrument was used to facilitate the transfer of transcriptomic probes from the standard glass slide to the Visium CytAssist Spatial Gene Expression Slide, v2, 11 mm capture area. Probe hybridization, probe ligation, release, extension, pre-amplification, and library preparation followed the Visium CytAssist Spatial Gene Expression Reagent Kits User Guide (CG000495). Sequencing was performed on the NovaSeq 6000 (28 cycles Read 1, 10 cycles i7, 10 cycles i5, 90 cycles Read 2).