GSM8449535: cct1-2 DNase-Seq; Saccharomyces cerevisiae; OTHER
DNA was isolated, resolved on a 1% agarose gel, the DNAs in the size range of 100-650 bp were excised. Gel-excised DNA was purified using a QIAprep Spin Miniprep columns (Qiagen). The libraries were prepared with Illumina's 'TruSeq DNAseq Sample Prep kit' with one modification: adaptors were diluted 1:20. The libraries were quantitated by qPCR, and sequenced on two lanes for 101 cycles on a HiSeq2000 using a TruSeq SBS sequencing kit version 3 and processed with Casava 1.8.2. The samples were sequenced on Illumina HiSeq2000.