GSM9635497: sci-RNA-seq3_Arabidopsis_Day0_to_Day4_Biological_Replicate_1_Technical_Replicate_1; Arabidopsis thaliana; RNA-Seq
Frozen tissue from each time point was crushed using a bead bashing approach, and nuclei released from homogenate by resuspending in Nuclei Buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl2). The resulting suspension was passed through a 30-um filter. Library construction was performed following the sci-RNA-seq3 workflow