CSR sequencing of mus musculus
CSR junctions were amplified as described (Boyer et al, Journal of Immunology 2017) and used to  prepare next generation sequencing (NGS) libraries (Ion Xpress Plus Fragment Library Kit, Life technologies, Thermofisher, 447269). Briefly Libraries of 200-bp read length were prepared from 100 ng equally mixed triplicate PCR products as a starting material. Library concentrations were determined by Qubit DsDNA high sensitivity (Invitrogen), and an equimolar mixture was prepared. Libraries were sequenced with an Ion S5 chip (Life Technologies). FastQ were analyzed using CSReport (Boyer et al., Journal of Immunology 2017).