GSM9370995: Sh3-1A; Homo sapiens; RNA-Seq
The concentration of RNA in each sample was measured using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The OD260/OD280 ratio was used as an indicator of RNA purity. RNA samples were considered pure if the OD260/OD280 ratio ranged from 1.8 to 2.1, with the corresponding QC Results marked as "Pass". Denaturing agarose gel electrophoresis was performed to assess RNA integrity and genomic DNA (gDNA) contamination. Library quality was evaluated using an Agilent 2100 Bioanalyzer. Briefly, total RNA was used for removing the rRNAs with NEBNext rRNA Depletion Kit (New England Biolabs, Inc., Massachusetts, USA) following the manufacturer's instructions. RNA libraries were constructed by using NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, Inc., Massachusetts,USA) according to the manufacturer's instructions. Libraries were controlled for quality and quantified using the BioAnalyzer 2100 system (Agilent Technologies, Inc., USA). Library sequencing was performed on an Illumina Novaseq instrument with 150bp paired end reads