GSM8650290: Trigeminal ganglia, pooled, HH1516, biological replicate 2, technical replicate 1; Gallus gallus; RNA-Seq
Total RNA was isolated from pooled trigeminal ganglia using RNAqueous Total RNA Isolation kit (ThermoFisher). RNA concentration and integrity were quantified with Nanodrop (ThermoFisher) and Agilent 2100 Bioanalyzer. PolyA-enriched cDNA libraries were prepared using TruSeq sample preparation kit (Illumina), followed by assessment of quality and quantity with the Bioanalyzer.