GSM8853901: P1P6MLL_WT_morula_embryos_Rep4; Mus musculus; RNA-Seq
For bulk RNA-seq of PRC1-WT and -CD FGOs, ten oocytes were pooled to generate each RNA-seq library. The zona pellucida was removed by brief incubation in acidic Tyrode's solution. All samples for RNA-seq were washed three times in 0.2% BSA/PBS before being transferred into a 0.2-ml tube with ~0.5μl carryover. For RNA-seq, libraries were prepared as previously described (Inoue et al., Genes Dev. 2018). Briefly, cDNA was prepared by SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (TAKARA, 634890) according to the manufacture's instructions. Two hundreds picograms of cDNA were used for the library construction by using Nextera XT DNA Library Preparation Kit (Illumina, FC-131-1024) according to the manufacture's instructions.