GSM9202281: dFlox2M1Dex; Mus musculus; RNA-Seq
The cell lysates were collected after the 4 hour Vehicle or Dex treatment. Total RNA was isolated from the primary macrophages using Qiagen RNeasy mini kit. The integrity of the total RNA was determined using the TapeStation (Agilent Technologies), and RNA concentration was assessed using a Qubit (ThermoFisher Scientific).  A TruSeq RNA kit (Illumina, San Diego, CA) was utilized to prepare the poly(A)-enriched RNA-seq libraries that were sequenced on the Illumina NovaSeq 6000 in a 75-base paired-end mode according to the manufacturer's protocol.