RNA-Seq of homo sapiens: adult glioblastoma
RNA was extracted from cell pellets using the RNeasy kit (Qiagen), quantified using a Qubit kit (Thermo Fisher Scientific) purity was assessed by 260/280 and 260/230 ratios using a Nanodrop (Thermo Fisher Scientific) . Libraries were prepared for mRNA analysis and sequenced on an Illumina Novaseq X using 150bp paired - end mode by Novogene Corp Inc., CA.