Methylation-sensitive SMiLE-seq sequencing data (illumina) of human transcription factors profiled in the Codebook/GRECO-BiT collaboration
Methylation-sensitive SMiLE-seq sequencing data (illumina) of human transcription factors profiled in the Codebook/GRECO-BiT collaboration
Input libraries were sequenced without any filtering. Eluted libraries (data from experiments) underwent selection based on TFs used in the SMILE-seq procedure. DNA libraries were collected after the SMiLE-seq procedure, purified and amplified via PCR. The amplified libraries were size selected using AMPureXP beads and amplified with i5 and i7 Nextera adapters to prepare for sequencing. Libraries were ordered as hand-mixed DNA oligos from IDT and synthesized via enzymatic reaction using DNA Polymerase I (Large Klenow Fragment). DNA libraries that contained the methylation barcode were enzymatically methylated using the M.Sss I methyltransferase.