GSM8448721: HC_T_NT_R9; Homo sapiens; RNA-Seq
RNA was isolated from Hofbauer cells using the RNAeasy kit (Qiagen, Hilden, Germany). Nucleic acid concentration and purity were assessed using a Nanodrop (Thermo Fisher) and Bioanalyzer (Agilent). RNA integrity numbers (RIN) <7 were excluded from further analysis. Stranded mRNA libraries were constructed involving mRNA enrichment, RNA fragmentation, cDNA synthesis, end repair, A-tailing, adaptor ligation, PCR amplification, single-strand separation, cyclization, and DNA nanoball synthesis.