Potter Cove sediment slurry SIP incubation - 13C Acetate.Lepidocrocite at 25 degrees C - RNA Light fraction - Bacteria 16Sr RNA
RNA was extracted from slurry with a phenol-chloroform protocol. RNA was density separated into 14 fraction by ultra-centrifugation. Selected fractions were pooled for cDNA synthesis. PCRs were performed using primers with unique 8 bp barcodes at forward and reverse primers. Multiple samples were pooled into one library for sequencing. Some samples were sequenced on multiple sequence lanes and libraries in order to retrieve sufficient reads; during demultiplexing reads were sorted by read direction forward-reverse or reverse-forward using the primer sequence, resulting in multiple paired fastq files (=runs) for the same ENA experiment