GSM8785874: WT, replicate 1(ChIP); Mus musculus; ChIP-Seq
Tissue was washed in cold PBS and cross-linked with 1% formaldehyde for 10 minutes and then quenched by addition of glycine. Afterwards, samples were lysed and chromatins were obtained on ice. Chromatins were sonicated to get soluble sheared chromatin (average DNA length of 200-500 bp). Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the I NEXTFLEX ChIP-seq Library Prep Kit for Illumina Sequencing（NOVA-5143-02，Bio Scientific）and sequenced on Illumina Novaseq 6000 with PE 150 method