GSM8449263: 11325GEPool01-N__Decid_tissue; Mus musculus; RNA-Seq
Lesions were dissected, pooled from each mouse and placed in 2ml ice-cold DMEM. Tissues were cut into small pieces using a scalpel and digested. Peritoneal lavage cells were recovered by injected 7ml ice cold RPMI into the peritoneal cavoity, followed by gentle massage and recovery. RBCs were lysed and cell were blocked. 200,000 CD45+ cells were FACS sorted into 1ml PBS + 2% FBS using a FACS Fusion. Cells were barcoded using a 10X Genomics Chromium ControllerTM using established pipelines