ITS amplicon data from Carex aquatilis roots planted in oil sand process affected water
Two grams of roots were cleaned with a series of sonication, cleared of debris using forceps and transferred into new 50-mL Falcon tubes, containing ~10 mL of fresh PowerBead solution. Cleaned roots were pat dried and cut into small pieces. 100 mg of roots were transferred into 2 mL Eppendorf Safe-Lock tubes, each containing one sterile 5 mm tungsten bead (QIAGEN), frozen in liquid nitrogen and stored at -80 C. Just prior gDNA isolation, frozen roots were ground into a fine powder using the TissueLyser II (QIAGEN) at 26 Hz for  2 xmin 45 s, with snap-freezing  by immersing the tubes in liquid nitrogen between grinding cycles. Isolation of DNA was performed with the DNeasy PowerSoil Pro kit. Fungi were targeted by sequencing the ITS2 region using the primer pair ITS9F  and ITS4R.