RNA-Seq of Callicarpa nudiflora: mature leaf
The mRNA of eukaryotic transcriptomes is enriched using Oligo(dT) beads based on its polyA tail. The mRNA is then fragmented and used as a template to synthesize first-strand cDNA with random primers, followed by second-strand cDNA synthesis using DNA polymerase. The double-stranded cDNA is purified, end-repaired, A-tailed, ligated with adapters, size-selected, and enriched by PCR to obtain the final cDNA library.