GSM8964738: FXS6; Homo sapiens; RNA-Seq
RNA was extracted using TRIzol™ Reagent (ThermoFisher Scientific # 15596026). The RNA obtained was treated with TURBO™ DNase (Invitrogen # AM2238) and was further purified using the RNA clean and concentrator kit (ZymoResearch # 11-325). RNA quality and abundance were assessed using fragment analysis (Agilent/Advanced Analytical). The quality scores ranged from 3.3 to 6.1 across the samples. Strand-specific RNA-seq libraries were constructed with 500ng of total RNA using ribosomal RNA depletion and the NEBNext Ultra II Directional RNA library prep kit for Illumina. Samples were PCR amplified for 9 cycles and sequenced using the Illumina NextSeq 2000 P4 XLEAP-SBS kit with 2 x 100 nucleotide paired-end reads.