Shotgun metagenomic sequencing of Vaccinium myrtillus: Tromso replicate 2 leaf sample
Genomic DNA was extracted from the leaf surfaces of Vaccinium myrtillus using the DNeasy PowerSoil Kit (QIAGEN) according to the manufacturer's instructions. Shotgun metagenomic libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit with random fragmentation and dual indexing. Libraries were size-selected to enrich for ~350 bp fragments and quality-checked using Bioanalyzer. Paired-end sequencing (2  300 bp) was performed on the Illumina MiSeq platform.