RNA-Seq of Synechococcus elongatus: wild-type cells at ZT16, replicate 1
Total RNA was extracted and rRNA was depleted. First-strand cDNA was synthesized with random hexamer primers. Second-strand synthesis used dUTPs for strand specificity. Libraries were prepared via end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Quality was assessed by Qubit, qPCR, and Bioanalyzer. Sequencing was performed on the Illumina platform with paired-end reads.