Pacbio HiFi sequencing of Thinopyrum intermedium for genome assembly
High-molecular weight genomic DNA of Thinopyrum intermedium was sheared to a target size of approximately 20 kb. Library preparation was performed using the SMRTbell Express Template Prep Kit 2.0. The resulting libraries were sequenced on a Pacific Biosciences Sequel II platform in CCS mode. Raw data were processed into high-precision HiFi reads.