HTAS snails and trematodes
The workflow simultaneously amplify one nuclear and three mitochondrial markers of the snails, and one nuclear and four mitochondrial markers of the trematodes. These included two fragments of the snails cytochrome c oxidase subunit 1 (COI1_snail and COI2_snail), a fragment of NADH dehydrogenase subunit 1 (NAD1_snail), a fragment of the internal transcribed spacer 1 of the rRNA cluster (ITS1_snail) and for the trematodes two fragments of COI (COI1_trematode and COI2_trematode), one fragment of NAD1 (NAD1_trematode), one fragment of cytochrome b (cytb_trematode) and one fragment of internal transcribed spacer 2 (ITS2_trematode). Details in https://doi.org/10.1111/1755-0998.13752