GSM9100173: Splenic CD8+ T cells, But, rep2; Mus musculus; ssRNA-seq
Viable CD8+Vα2+ single cells from control and butyrate treated gBT-I cells in vitro cultures were purified by flow cytometry using a BD Influx (> 98% purity), resuspended in 0.04% BSA and filtered through a 70 µm sieve before being captured in droplets for library preparation. Library preparation was done using the 10X Genomics Chromium Single Cell 3' V3.1 chemistry.