GSM8888989: A431, parental, rep 1; Homo sapiens; RNA-Seq
Cell lysis and RNA isolation was done using Norgen's total RNA purification kit (#17200, Norgen Biotek) Indexed PolyA RNA-Seq libraries were prepared using 200ng of total RNA and 14 cycles of amplification with the Agilent SureSelect Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent Technologies, Inc., Cat No: G9691A). Libraries were quality checked by Bioanalyzer (Agilent) and quantified by qPCR using a Kapa Library Quantification Kit (Kapa Biosystems Inc., Cat No: KK4835).