RNA-seq of rattus: sample -H-2
16S rRNA amplicon sequencing was performed by Genesky Biotechnologies Inc., Shanghai, 201315 (China). Briefly, total genomic DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to the manufacturer's instructions. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified with the primers . Then sequenced the purified amplicons using Illumina NovaSeq 6009 sequencing platform.