GSM9042863: 2.5_3h_WT_IntS11_mislocalized_Micro-C; Drosophila melanogaster; Hi-C
Embryos were dechorionated for 5 min in 50% bleach and rinsed with PBS. The embryos were resuspended in PBST (PBS with 0.1% Triton X-100) and fixed with 3 mM DSG for 15 min at room temperature. The embryos were then subjected to a second crosslinking step using freshly prepared 1% formaldehyde at room temperature for 30 minutes, followed by washing with wash buffer. Subsequently, the embryos were resuspended in wash buffer and homogenized with a loose stroke 100 times, followed by centrifugation at 2000 g for 5 minutes at 4℃ to collect the pellet. The cell pellet was digested with MNase and subsequent steps were carried out according to the kit's protocol. Micro-C was performed using a Dovetail Micro-C kit (Cantata Bio) according to the manufacturer's protocol.