GSM9017902: Breast cancer; Homo sapiens; miRNA-Seq
Total RNA was extracted from the samples using the MagZol Reagent kit (Magen Company). The RNA concentration was detected by a nanodrop instrument, and the RNA fragment distribution was analyzed by an Agilent 2200 instrument. The NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, USA) kit was used to construct small RNA libraries.