Bisulfite-Seq of 14DAP endosperm from WT-rep3 (for zmros1ab)
The standard CTAB method was used to prepare DNA for whole-genome bisulfite sequencing. Libraries were constructed using 1 g of genomic DNA based on a previously published protocol (Li et al., 2018). Library quality was checked using Qubit and gel electrophoresis. Sequencing was performed on Illumina NovaSeq X plus under the paired-end mode.