RNA seq of Dunaliella salina cultured under red light for 15 days
After the sample testing is qualified, the library construction is carried out, and the main process is as follows: (1) Enrichment of eukaryotic mRNA using magnetic beads with Oligo (dT); (2) Add Fragmentation Buffer to randomly interrupt mRNA; (3) Using mRNA as a template, synthesize the first and second cDNA strands, and purify the cDNA; (4) The purified double stranded cDNA was subjected to terminal repair, followed by the addition of an A tail and ligation of sequencing adapters, and then fragment size selection was performed using AMPure XP beads; (8) Finally, the cDNA library was obtained by PCR enrichment.