mRNA-seq_UT-1
Total RNA was extracted from HEK293T cells transfected with RNA editors and targeting or non targeting guides, with untransfected cells as controls. RNA quality was confirmed (RQN > 8). PolyA mRNA was isolated using the NEB mRNA Isolation Kit, and stranded RNA seq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit. Libraries were sequenced on an Illumina NovaSeq X platform (2 x 150 bp) with a target depth of 40 million clusters per sample. The data were used for transcriptome wide detection of A to I editing events and to compare off target editing profiles between Cas13 based REPAIR and Cas9 based RETREAT systems.