Pig 16S
Five l of diluted DNA from each library were mixed for pooling libraries and denatured with NaOH, diluted with hybridization buffer and heat denatured. Five % PhiX served as an internal control. For sequencing on the MiSeq Illumina platform instrument, a v3  v4 Miseq sequencing kit (2250 bp paired-end reads; Illumina) was used.