GSM9156514: U251-D28W, biol rep 3; Homo sapiens; RNA-Seq
RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA integrity was verified using an Agilent Bioanalyzer 2100 (RIN > 8). RNA libraries were prepared using the TruSeq RNA Library Prep Kit (Illumina) and sequenced on an Illumina HiSeq 2500 to generate 150 bp paired-end reads.