16S amplicon data from Carex aquatilis roots planted in oil sand process affected water
Two grams of roots were cleaned with a series of sonication, cleared of debris using forceps and transferred into new 50-mL Falcon tubes, containing ~10 mL of fresh PowerBead solution. Cleaned roots were pat dried and cut into small pieces. 100 mg of roots were transferred into 2 mL Eppendorf Safe-Lock tubes, each containing one sterile 5 mm tungsten bead (QIAGEN), frozen in liquid nitrogen and stored at -80 C. Just prior gDNA isolation, frozen roots were ground into a fine powder using the TissueLyser II (QIAGEN) at 26 Hz for  2 xmin 45 s, with snap-freezing  by immersing the tubes in liquid nitrogen between grinding cycles. Isolation of DNA was performed with the DNeasy PowerSoil Pro kit. Prokaryotes were targeted by sequencing the V4-V5 region of the 16S rRNA gene  with the 515F-Y  and 926R primer pair.