sRNA-Seq of mango: mesocarp 3 h
Total RNA was extracted from the mango samples (Lpez-Gmez and Gmez-Lim, 1992) and treated with DNase I. Two grams of RNA from samples collected at 1, 3, 6, and 24 hours post-HWT were sent for library preparation and sequencing (Novogene Co.). Briefly, 3 and 5 adaptors were ligated to the 3' and 5' ends of small RNA, respectively. Then, the first strand cDNA was synthesized after hybridization with the reverse transcription primer, and the twelve small-RNA libraries (three biological replicates per time point) were generated through PCR enrichment, purified, and size selected.