GSM8787567: G000304_2_GEX; Mus musculus; RNA-Seq
Lymph nodes were harvested and processed to form a single cell suspension. Samples were stained for surface antigen expression using indicated antibodies and TotalSeq HashTags (BioLegend) for 30 minutes at 4°C, followed by viability dye staining for 10 minutes at 4°C. Sample suspensions were sorted using BD FACSAria Fusion Flow Cytometer (BD Biosciences) to isolate antigen specific P14 CD8+ T cells. The library prep was done according to the 10X Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) CG000315. Sequencing was done on the NextSeq2000 on a P3 flowcell.