GSM9458511: Upstream tandem hiPSC, uninduced, Pol II (ser2), rep2; Homo sapiens; OTHER
Cells were bound to ConA beads alongside spike-in Drosophila nuclei, then permeabilized and bound with one of four primary antibodies: Pol II (ser2) (Cell Signaling Technologies 13499S), H3K27ac (Active Motif 39133), H3K27me3 (Active Motif 91167), or H3K4me3 (Active Motif 39915). Cells were prepared following the CUT&Tag-IT Express kit (Active Motif 53177). Nextera-compatible combinatorial dual-index primers were used to index the libraries (Active Motif 53155).