GSM8449363: ChIP-Seq - SUM149 cells, Control (endogenous ERβ),  INPUT, replicate 1; Homo sapiens; ChIP-Seq
KPL4 cells with different ERβ levels (Control, ERβKO and ERβΚΟ+ERβ) were developed and characterized as previously described in Al Absi et al. (2018). Control and ERβ-expressing SUM149 cells were generated by infection with lentivirus containing the pLenti6/V5-FLAG-ERβ1 (full length ERβ) construct. Cells were maintained in HAM's-F12 media (Corning) supplemented with 7% fetal bovine serum (FBS) (Sigma), 10 mM HEPES (Thermo Fisher), 5 μg/mL insulin (Sigma), 1μg/mL hydrocortisone (Sigma) at 37°C in 5% CO 2. For ligand treatments, cells were cultured in phenol red-free media supplemented with 1% dextran-coated charcoal-treated FBS (Thermo Fisher) for 72 hours prior to treatment with the ERβ agonist LY500307 (ApexBio) or DMSO (Sigma) for 2 hours in the same media. All the cell lines were tested negative for mycoplasma, authenticated by short tandem repeat (STR) profiling and used within three passages after thawing. ChIP libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7645). Quality control of the final libraries was performed on Agilent 2100 Bioanalyzer (Agilent Technologies)