ITS amplicon sequencing of fungal community from stem of Sorghum bicolor cv. Kalatur treated with Trichoderma
Samples were collected from roots, stems, seeds, and rhizosphere soils of Sorghum bicolor cultivars Foehn and Kalatur grown under control and bioinoculated conditions. Fungal DNA was extracted and the ITS2 region was amplified using universal ITS primers. Sequencing was performed on an Illumina MiSeq platform to assess fungal community diversity and structure.