RNA-Seq of Greenland shark (Somniosus microcephalus): adult retina 2
Retinal transcriptomes were sequenced for three individuals for S. microcephalus. Briefly, total RNA was extracted from one RNAlater-fixed retina from each of three adult sharks using the QuickRNA Miniprep kit (Zymo). RNA quality was assessed on an Agilent TapeStation and library preparation and sequencing was performed by the Department of Biosystems Science and Engineering (ETH Zurich). Individual libraries were prepared using TruSeq stranded total RNA ribo-zero gold kit (Illumina), pooled, and sequenced as 100-bp paired-end reads using BRAVO Sequencing on an Illumina NovaSeq6000 with an S4 flow cell (200 cycles).