GSM8785850: H3K27AC-AK58-spike_rep1; Triticum aestivum; ChIP-Seq
Total RNA was extracted with TRIzolTM Reagent (Invitrogen; 15596-026), in ATAC-seq the nuclei were obtained using flow cytometry, in Chip-seq the wheat spike cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using an  focused-ultrasonicator (Covaris). All library was constructed by Berry Genomics (Beijing, China), and sequenced with Illumina NovaSeq 6000. All sequencing data were 150bp paired-end reads.