GBS of Zea mays: Ab10, K10L2, B Chromosome, and negative control diversity panel
We sent leaf tissue to CD Genomics (Shirley, NY) who extracted DNA using QIAgen DNeasy Plant Kits. They prepared GBS libraries as in https://doi.org/10.1371/journal.pone.0019379 with minimal modification. Basically this involved digesting DNA with ApeKI (New England Biolabs, Ipswich, MA), adding barcoded adapters, and sequencing the libraries on an Illumina NovaSeq6000 using a 1502 paired-end sequencing protocol. These are the raw multiplexed files (for ease of use with GBS specific tools such as TASSEL https://doi.org/10.1093/bioinformatics/btm308).  Refer to https://github.com/dawelab/CDH_Distribution_In_Maize for a full sample description and the key used to demultiplex the fastq file.