GSM8919175: TdTomato+ cells collected from +/+;Aldh1l1+;tdT+/- 9 weeks old mouse brain (Replicate 2); Mus musculus; RNA-Seq
Cortices were isolated at postnatal week 9 from animals injected with tamoxifen at postnatal week 5 and collected for single tdT+ cell sorting. Differently, the single cells were directly sorted into a 1.5 mL Eppendorf tube containing 750 μL of Trizol LS (Thermo Fisher Scientific #10296028). Cell sorting in each tube proceeded until volume reached 1mL and then a new tube with Trizol LS was replaced until the end of the sorting. Total RNA was extracted according to manufacturer's instructions for Trizol reagent. The RNA quality was monitored by Agilent TapeStation 4150. The libraries were prepared using ABClonal mRNA-seq Lib Prep Kit for Illumina by Novegene Corporation Inc.