GSM8888329: lncMGC KO high fat diet_4; Mus musculus; OTHER
FFPE blocks were cut into 5μm sections and each samples were placed on the same slide for subsequent simultaneous analysis. Slides were hybridized with RNA probes and stained for UCP-1, CD45, and nuclei. Using the GeoMx DSP instrument, selected regions of interest (ROIs) were segmented into following areas: 1.) UCP1 postive and CD45 negative area, 2.) CD45 postive area and 3.) remaining DNA postive area, and the hybridized RNA probes were collected separately from these three areas. Following the protocol described (GeoMx® DSP NGS Readout) PCR reactions were purified and libraries were paired-end sequenced (2x50) on a NextSeq 2000.