GSM8677444: G23_GM, ATAC; Homo sapiens; ATAC-seq
Nuclei were isolated using the Chromium Nuclei Isolation kit (10X Genomics) according to the manufacturer's protocol (CG000505, revision A), which is compatible with the Single Cell Multiome ATAC + Gene Expression assay.  Briefly, frozen tissue samples were dissociated with a pestle in lysis buffer and passed through a nuclei isolation column. The isolated nuclei were then resuspended in a debris removal buffer, followed by washes to clear any remaining debris. Recovered nuclei were stained with Trypan blue and counted with a hemocytometer. Nuclei integrity was evaluated under a microscope at 40X or 60X magnification, and nuclei were considered viable if they were roundish and with an intact membrane. Libraries were prepared using the Multiome ATAC + Gene Expression kit (10X Genomics) according to the manufacturer's protocol CG000338 revision E.