16S rRNA amplicon of microbial community: PPSV-2 anodic biofilm
16S rRNA gene V3-V4 amplicon libraries were prepared from genomic DNA extracted from anodic biofilm samples. The V3-V4 region was amplified with primers 338F/806R using a two-step PCR protocol, followed by adapter/index addition, bead purification, Qubit quantification, equimolar pooling, and Illumina MiSeq paired-end 2  300 bp sequencing.