GSM8921828: AKTP MTO mouse tumor organoids, RM-044 6h, replicate 2; Mus musculus; RNA-Seq
RNA was extracted using TRIzol reagent followed by chloroform phase separation. Total RNA was subjected to alkylation by iodoacetamide (Sigma, 10mM) for 15 min and RNA was repurified by ethanol precipitation. 500ng alkylated RNA were used as input for generating 3'-end mRNA sequencing libraries using a commercially available kit (QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina and PCR Add-on Kit for Illumina, Lexogen). Deep sequencing was performed using NovaSeqX platforms (Illumina).