Mus_musculus_hippocamp_Gld2_KO_rep2_ONT_DRS
Total hippocampal RNA was extracted with RNeasy Lipid Tissue Mini Kit (Qiagen), purified with KAPA Pure Beads (Roche) and quality checked on Bioanalyzer 2100 (Agilent). 4.5-5 ug of total RNA was mixed with 150-200 ng of oligo(dT)25-enriched mRNA from Saccharomyces cerevisiae, and spiked with 5 ng of in vitro synthesized poly(A) standards. Sequencing libraries were prepared with Direct RNA Sequencing Kit (Oxford Nanopore) SQK-RNA002. Sequencing was performed on a MinION device equipped with R9.4.1 FLO-MIN006 flow cell and controlled with MinKNOW software.