GSM9017649: day 20 Hepato-biliary Organoids in Maturation Medium, Donor 16087; Homo sapiens; RNA-Seq
Total RNA was isolated from organoids, tissue and primary cells using the RNeasy Mini Kit (QIAGEN) following the manufacturer's instructions. The isolated total RNA was passed through an additional round of DNase treatment using the Zymo Research Direct-zol RNA Miniprep kit (Cat. #R2050) 250ng of total RNA was used as input per sample to prepare mRNA library with the Kapa mRNA HyperPrep Kit (Roche Cat. #08098123702). 12 PCR cycles were used to generate the amplified cDNA library as per the kit recommendations.