Stranded RNA-seq of LdoS after 24 hr of axenic differentiation (rep 2)
L. donovani promastigotes were exposed to the differentiation signal                 (37C, pH 5.5) and grown for the time indicated before RNA was isolated using TriZOL                 (Invitrogen). Purified RNA was treated with 20 units of DNase I (Ambion) and was                 precipitated using 1/10 volumes 3M sodium acetate (Sigma) and 3 volumes 100%                 ethanol. RNA was pelleted by centrifugation at 15,000 g-1 and the resulting pellet                 was washed with cold 75% ethanol and resuspended in RNAse-free water (Ambion).                 TruSeq Stranded Total RNA Library Prep Kit from illumina and its standared protocol                 was used to prepare RNAseq libraries from total RNA.