Sham-Isotype (Iso) 1 Month (1m) post-TBI, delayed treatment, Biological Replicate #5
Bulk RNA-sequencing analysis was performed on sorted microglia single cell suspensions from the ipsilateral hemisphere of the mouse brains using the microglia-specific 4D4+ antibody at 30 days following traumatic brain injury. Preparation for RNA libraries for sequencing was performed using standard Illumina protocols. For the delayed treatment experiment, the therapeutic window of nasal anti-CD3 treatment was delayed to 3 days following injury.