GSM9049709: RNA Isogenic2; Homo sapiens; RNA-Seq
D100 organoids were transferred from culture plates to 1.5 mL tubes and briefly washed in ice-cold DPBS. For each biological replicate, 3 organoids were pooled in the same tube. After complete removal of DPBS, organoids were homogenized using QIAshredder homogenizers (Qiagen, #79656), and total RNA was extracted using the RNeasy Micro Kit (Qiagen, #74004) according to the manufacturer's instructions. Library preparation and RNA sequencing were performed as a service by GENEWIZ/AZENTA and sequenced on a NovaSeq 6000 instrument (Illumina).