GSM8503712: Lmod2_pro_wt_rep1; Mus musculus; OTHER
The thoroughly ground heart tissue was cross-linked with 2% formaldehyde and quenched with 0.2 M glycine. DNA was then digested with nuclease in situ. 500 ng of DNA was used as a template and linearly amplified using 5' biotin-labeled probes. The ssDNA was enriched using Dynabeads™ M-280 magnetic beads. Illumina sequencing adaptors were introduced, followed by PCR using target-specific primers. Sequencing was performed on the Illumina NovaSeq platform.