GSM8920646: Lung, TTI-101, 4; Mus musculus; RNA-Seq
Mouse lung RNA was extracted by placing cryopreserved lung tissue into tubes containing chrome plated steel beads and QIAzol (Qiagen). Tubes were then shaken on a Mini-BeadBeater 16 (BioSpec) for 10-20 seconds twice, incubating samples on ice between each round. Homogenates were then passed through a QIAshredder (Qiagen) for further cellular lysis. RNA extraction was then performed using the RNeasy Mini kit (Qiagen) following the manufacturer's instructions. RNA libraries for RNA-seq were prepared using Illumina's TruSeq Stranded mRNA Library kit (Cat# 20020594)