GSM8503026: anti-PD1,replicate 2, scRNAseq; Mus musculus; RNA-Seq
Fresh bladder tissues were collected from BBN-induced bladder carcinogenesis mice. For the quality check and counting of single cell suspension, the cell survival rate is generally above 80%. The cells that have passed the test are washed and resuspended to prepare a suitable cell concentration of 700~1200 cells/ul for 10x Genomics ChromiumTM. The system is operated on the machine. Sorted cell suspension from mouse bladder was loaded on a well of 10x Chromium single cell instrument (10X Genomics). Barcoding, cDNA synthesis and library construction were administered using the manufacturer's instructions.