GSM8785822: Cryptocercus punctulatus was dissociated into single nuclei; Cryptocercus punctulatus; RNA-Seq
One adult specimen of Cryptocercus punctulatus was kept in the Bourguignon lab at Okinawa Institute of Technology, Japan. After slicing with ethanol-cleaned scissors and pestle maceration (~20 strokes) in 1 ml homogenisation buffer (Supplementary Table 4), the slurry was centrifuged for 10 minutes at 1000g and 4 ℃, and resuspended in 1ml PBS 0.5% BSA, then filtered through a 100 μm (Falcon 352360) and 35 μm cell strainers to eliminate large debris and immediately moved to the precalibrated sorter (Sony SH800S). 0.1% DAPI was added and nuclei were sorted within 20 minutes using a gate DAPI+ FSClow. After sorting, nuclei were inspected under an epifluorescence microscope, pelletted and resuspended after removing 700 μl of supernatant, then finally adjusted to 700-1200 nuclei / μl using a hemocytometer. Libraries were constructed using a 10X Genomics Chromium with 3' chemistry v3 and dual-index Nextera-equivalent barcodes, and sequenced at a total depth of 621,418,196 reads pairs.