GSM8920567: Rv0042c IDAPSeq: 300nM protein, replicate 2; Mycobacterium tuberculosis; OTHER
See publication for complete details. Sheared Mtb genomic DNA (mean ~300 bp) was incubated with N-terminal HIS tagged SlfR protein. Protein:gDNA was enriched by binding to Talon resin and washing off unbound gDNA. Bound gDNA was eluted. Eluted gDNA was prepared for Illumina sequencing using an NEBNext Ultra II DNA Library Prep Kit.