GSM9597760: Control (AcGFP) human adipose-derived stem cells replicate 2; Homo sapiens; RNA-Seq
Total RNA was extracted using GeneJET™ RNA Purification Kit  according to the manufacturer's instructions. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Libraries were prepared by end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Library quality was assessed using Qubit, real-time PCR, and bioanalyzer, and libraries were sequenced on an Illumina platform.