GSM8594717: WT_replicate 1; Yersinia pseudotuberculosis; RNA-Seq
Strains were grown overnight  at 26°C and subsequently diluted 1:25 into fresh media and cultured for 5h at 26°C at which point samples were taken. Total RNA was extracted using a Trizol RNA isolation kit according to the manufacturer's protocol. RNA concentration was measured using Qubit® RNA Assay Kit in Qubit® 2.0 Flurometer (Life Technologies, CA, USA). RNA was sent to Novogene Bioinformatics Institute, there, RNA degradation and contamination was monitored on 1 % agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). RNA integrity and quantitation was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).