GSM8449001: Pool 12 of 4 specimens; Mus musculus; RNA-Seq
Tumors were harvested and frozen in DMEM/10% FCS with 10% DMSO. We isolated RNA using the RNeasy (QIAGEN) RNA extraction kit. Library preparation was performed using NextGEM single cell 5' version 2 chemistry and according to the manufacturer's protocol (10x Genomics, CG000330). Four biological specimens were pooled in each library using TotalSeq-A hashing antibiodies (BioLegend).