GSM8965581: CREB_antibody_E165 testis; Mus musculus; OTHER
Male gonads were dissected and dissociated into individual cells by pipetting. The resulting cell suspension was filtered using a 70 µm filter, and 100,000 gonadal cells were used for CUT&RUN assays following an alternative protocol from Protocol.io (dx.doi.org/10.17504/protocols.io.zcpf2vn). DNA was quantified and prepared for sequencing using the NEBNext Ultra II DNA Library Prep Kit for Illumina.