E coli
Shotgun metagenomic libraries were prepared Illumina DNA Prep (M)                 Tagmentation protocol. Libraries were amplified with 5 PCR cycles and validated with                 fragment size distribution averaging 534 bp (range: 462-586 bp). Paired-end                 sequencing (2x151 bp) was performed on Illumina NovaSeq 6000 using S2 flow cell with                 1% PhiX control.