Nanopore sequencing of gDNA from Scheffersomyces stipitis isolate NRRL Y-50859
DNA was extracted from an overnight culture using a Qiagen genomic tip 100/G kit (Qiagen, catalog no. 10243) according to manufacturers protocol. MinION (Oxford Nanopore, Oxford, UK) sequencing was performed on a DNA library prepared from size-selected gDNA. DNA fragments greater than 30 kb were selected using a Blue Pippin (Sage Science) and concentrated using AmPure beads. From this, a DNA library was prepared using a 1D ligation sequencing kit (SQK-LSK108) and run on the Oxford Nanopore MinION flow-cell FLOMIN 106D.