+2DG_Day 10_Replicate3
Overnight culture of S. cereviae wild-type 1278b was spotted on nitrogen-limiting media containing 2% (w/v) glucose (SLAD) with and without sub-inhibitory concentration of 2-Deoxy-D-Glucose (2DG). The plates were incubated at 30 C and cells from colonies were collected on day 5 (D5) and day 10 (D10) for RNA isolation.