RNA-Seq of FCMF strain: rhizoid2
Total RNA was extracted from the rhizoid colony of the FCMF strain using the RNeasy Mini Kit (Qiagen, Hilden, Germany). The extracted RNA was then treated with RNase H to remove ribosomal RNA and subsequently fragmented. cDNA was synthesized using random primers, purified, and subjected to end-repair and 3' adenylation. Adapters were ligated to the adenylated cDNA fragments, followed by paired-end sequencing (100 bp reads) on the MGISEQ-2000 platform (MGI Tech Co., Ltd., Shenzhen, China).