GSM8594166: LCKFREE_IAV_D10 targeted scRNAseq; Mus musculus; RNA-Seq
Spleens were harvested into Hank's balanced salt solution (HBSS) and mashed through a 70μm cell strainer. Red blood cell lysis was performed by resuspending cells in 1ml red blood cell lysing buffer (Sigma Aldrich, Cat. 11814389001) for 4 min before quenching with 9ml HBSS. Cells were then filtered with 70μm cell strainer Library was performed according to manufacter's instructions (BD Rhapsody Single-cell Analysis System)