scRNA-seq of human PDAC- female pancreas
10,000 cells were loaded onto the Chromium Next GEM Chip K.  Gene expression (GEX) and TCR libraries were generated according to the 10x protocol.  Briefly, after droplet generation, cDNA was generated by reverse transcription, purified using Silane DynaBead clean-up, and then amplified via PCR for sixteen cycles.  Paired 5 GEX and TCR libraries for each sample were constructed and sequenced on the NovaSeq (Illumina) platform at a depth of 50,000 reads per cell for the GEX libraries and 5,000 reads per cell with dual indexing for the TCR libraries.