GSM9049552: Day 7, intravenous injection, experimental repeat 1, CITE-seq (ADT); Mus musculus; OTHER
ILC2s were sorted into complete media, spun down, and resuspended in 1x PBS + 0.4% BSA. Samples were then counted and loaded for emulsion droplet construction on a 10X Genomic IX controller. Gene Expression and ADT/HTO library preparation was performed by combining the instructions in the TotalSeq A Dual Index Protocol and 10X Genomics Chromium Single Cell 3′ Reagent Kits V3.1. One quarter of the cDNA was used to build the gene expression libraries, and indexed using the 10X, Set TT A index plate. Gene Expression libraries were pooled based off the projected number of cells captured and sequenced using an Ilumina NextSeq 2000, aiming for 20,000 reads per cell. For the ADT/HTO libraries, one quarter of the cDNA generated was used to build the ADT/HTO libraries and indexed using the TotalSeq A indexes listed in the protocol. ADT/HTO libraries were pooled based on the number of cells captured and sequenced using an Illumina NextSeq 2000, aiming for 5,500 reads/cell. All libraries were sequenced together in a single sequencing run.