minION of Planococcus koreensis: gDNA
Genomic DNA isolation was completed from a culture grown in LB media using the Monarch Genomic DNA Purification Kit (#T3010S). The isolate was gram-variable, thus gDNA was prepared using the Gram-positive and Gram-negative extraction protocols and individually sequenced. Library preparation was performed using the Oxford Nanopore Technologies Rapid PCR Barcoding Kit 24 V14 (SQK-RPB114.24). Protocol was followed using the manufacturers protocol except for the number of PCR cycles which was set to 25 cycles. Sequencing was performed with Oxford Nanopore Technologies MinION Flow Cell R10 Version (FLO-MIN114)