A26 - olfactory bulb from autopsy sample
Total RNA was extracted using AllPrep Mini Kit (Qiagen, Germany), according to manufacture protocol. RNA integrity was accessed by RNA Screen Tape (Agilent technologies, EUA). The cDNA libraries were prepared by SELA (High-throughput Sequencing Facility, University of So Paulo) using QuantiSeq 3 mRNA-Seq Library Prep Kit (FWD) (Lexogen). Library inspection and quantification were performed by qPCR (Kappa Library Quantification Kit, EUA) and TapeStation 2200 (Agilent Technologies, EUA), respectively. Libraries were pooled and sequenced on NextSeq 550 (Illumina).