GSM9156789: HTO_MG; Mus musculus; RNA-Seq
Brains were excised from mice transcardially perfused with PBS, and CD11b+ CD45+ Ly6G- CD206- microglia were collected by CytoFlex SRT(Beckman Coulter, Inc). Single-cell suspensions were processed using a Chromium Controller (10x Genomics) to generate single-cell gel beads in GEMs. scRNA-seq libraries were prepared using the Chromium Next GEM Single Cell 3' Gel Bead and Library Kit v3.1, following the manufacturer's instructions. Sequencing was performed using a NovaSeq 6000 at the Laboratory for Research Support of the Institute of Bioregulatory Medicine at Kyushu University.