benign_FT_NON-BRCAmut_59
200-250ng of extracted DNA using the IDT xGen Methylation-Sequencing DNA Library Preparation Kit (IDT, Cat #10009824, Accel-NGS Methyl-Seq DNA Library kit (v3.0), Swift Biosciences, Cat. #30024). DNA was sheared following the manufacturers protocol to an average size of 250bp, and sheared DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo Research, Cat. #D5005) with an elution volume of 15 ul. Following adapter ligation, 6 cycles of library amplification were performed.