GSM9127044: WT96H; Mus musculus; RNA-Seq
Total RNA was extracted from snap-frozen liver tissue using TRIzol reagent (Life Technologies, Carlsbad, CA). For baseline (0 hour) samples, RNA was isolated from individual mice (n = 4 per genotype). For the 12 to 96 hour time points, RNA from three biological replicates per genotype per time point was pooled prior to sequencing (n = 3 mice pooled per group). Following Illumina library prep and RNA-seq protocols.