GSM8892316: BRC from B-lineage cells from MM6; Homo sapiens; OTHER
EDTA-anticoagulated BM aspirates were stained with the following combination of monoclonal antibodies prior to FACS: CD3-FITC, CD19-PeCy7, CD38-APCH7, CD45-V500, CD56-PE, CD138-V450, CD300e-APC and 7-AAD-PerCP (viability). For scRNA/BCR-seq studies, CD19+veCD138-ve B cells and CD138+ve PC (independently of CD19 expression) were sorted into 100 µL of Lysis/Binding Buffer (Thermo Fisher Scientific, Waltham, MA). Cells were isolated in a FACSAria IIb flow cytometer (Becton Dickinson Biosciences [BDB], San Jose, CA) and sorted into 100 µL of Lysis/Binding Buffer (Thermo Fisher Scientific, Waltham, MA) for subsequent sequencing studies. scRNA/BCR-seq was performed using 10X Genomics Single Cell 5′ Solution v1 and v2 following the manufacturer's protocol (10X Genomics, CA, USA). Libraries were sequenced in a NextSeq550 (Illumina, CA, USA) for gene expression and BCR data.