GSM8889220: Gingival tissue, SIIN, Day 7, rep2; Mus musculus; RNA-Seq
Tissues were homogenized in RLT buffer using a Beadblaster (Benchmark) and Omni Bead Ruptor ceramic bead tubes (1.4 mm), and RNA was extracted using a RNeasy mini kit (QIAGEN) libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina), and sequencing was performed on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads. The sequencing depth achieved an average of 30 million reads per sample.