regenerating_skin_rep2
Total RNA was extracted and ribodepleted using a pool of custom DNA oligos complementary to the sequences of planarian (Schmidtea mediterranea) rRNA molecules and RNaseH treatment. The remaining RNA transcripts were used to generate libraries using a Roche KAPA RNA Hyperprep kit and assessed for quality on an Agilent BioAnalyzer. The libraries were then sequenced using paired-end sequencing technology on an SP flowcell of the Illumina NOvaSeq 6000.