GSM9126838: DPTILs, Donor49, LowCelltrace, rep1; Homo sapiens; OTHER
Genomic DNA was isolated using Zymo's QuickDNA Miniprep Plus kit. Genomic DNA was split across 100 μl PCR reactions (22 cycles at 98 °C for 10 s, 60 °C for 10 s, and 72 °C for 25 s) with NEBNext 2× Master Mix and up to 3 μg of genomic DNA per reaction. PCRs were pooled together for each sample and purified using double-sided (SPRI)bead selection at 0.5× and 1.0×. Libraries were run on a 1% agarose gel to confirm amplicon size and quantified using Qubit's dsDNA High Sensitivity assay. Libraries were diluted to 2 nM, pooled together at equal volumes, run on a High Sensitivity D1000 tape (Agilent) to confirm amplicon size, and sequenced using Illumina's MiSeq Reagent Kit v2 (50 cycles). Primers used for the gDNA PCR are listed in Supplemental Table 4.