RNA-seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings
RNA-seq of 24hpf Zebrafish larvae comparing foxg1a mutants to WT siblings
Heterozygous mutant (5bp deletion 32 bp from canonical start codon; AAATG) foxg1a adult zebrafish in a dlx5-6;GFP background were incrossed and progeny collected. Dlx+ interneuron phenotype was used to identify homozygous (no interneurons in telencephalon) and heterozygous (reduced number of interneurons in telencephalon). Genotyping was confirmed with RNA-sequencing reads at the foxg1a gene locus to ensure correct genotyping. RNA was extracted from single 24hpf zebrafish using the Qiagen RNeasy Micro Kit (Qiagen, 74004). RNA concentration was measured using the Qubit reagent kit and a small sample was tested for RNA integrity using a bioanalyzer instrument. Only samples with an RNA integrity number of 9 or above were used in this experiment. Total RNA Library was repapred using the Illumina TruSeq Stranded Total RNA Library kit. Ribosomal RNAs were depleted using Ribo-Zero Gold.