Pichia kudriavzevii NBRC0584 genome
Genomic DNA was quantified using the QuantiFluor dsDNA System (Promega) and assessed for quality using the 5200 Fragment Analyzer System (Agilent Technologies). The DNA was sheared to ~1025 kb using the Megaruptor 3 (Diagenode), and a PCR-free library was prepared with the SMRTbell Prep Kit 3.0 (PacBio). Sequencing was performed on the PacBio Revio platform following the manufacturers protocol.