GSM9156745: BLZ_Jung_Tam_2; Mus musculus; RNA-Seq
Brains were excised from mice transcardially perfused with PBS and 500 cells of CD11b+ CD45+ Ly6G- CD206- microglia were collected into 30uL of direct lysis buffer (TaKaRa #635013) by CytoFlex SRT(Beckman Coulter, Inc). Libraries were prepared using 15uL out of 30uL lysate by CEL-Seq2(Hashimshony T et al.) method individually. 10nt of UMI followed by 9nt of cell barcode are in Read1 and 81nt of insert reads are in Read2.