GSM9047553: HLC19 cell line transfected with Empty control vector treated with DMSO_replicate 1; Homo sapiens; RNA-Seq
Medium overlaying matrigel-embedded spheroids was removed, and 1 ml of QIAzol reagent (Qiagen, USA) was added to dissolve matrigel and contained cells. From this moment on, the RNA extraction protocol has been carried out as per the manufacturer's instructions. RNA was then assyed for concentration and purity, and DNAse treatment was then carried out using the Turbo DNA-free kit (Ambion, Thermo Fisher Scientific, USA). Libraries were templated with the Ion Chef and sequenced using a 540 chip on the Ion GeneStudio S5 Prime system (Thermo Fisher Scientific,Waltham, MA, USA).