GSM8853881: fatal brains, multiplexed: Ctrl, VPA, VPA + Ro; Mus musculus; RNA-Seq
Briefly, viable cells were isolated from neural tissue using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec), following the manufacturer's protocol. The resulting single-cell suspension was sorted using the BD FACS Aria Cell Sorter to enrich for viable single cells. Library preparation was conducted according to the BD Rhapsody™ WTA Amplification Kit protocol. Initial sequencing was performed using an Illumina NovaSeq, and the Sample Tag sequences were subsequently sequenced on a separate Illumina MiSeq to enhance tag read coverage.