GSM9127727: Day63,head,female,rep2-2; Drosophila melanogaster; RNA-Seq
We strictly randomized all steps of RNA extraction, library preparation and sequencing. We extracted total RNA using the RNeasy Mini Kit (Qiagen, Inc., catalog number 74104). We depleted ribosomal RNA (rRNA) using the Ribo-Zero Gold Kit (Epicentre, Inc.). We barcoded cDNA from each RNA sample was using NEXTflex DNA Barcodes (Bioo Scientific, Inc.) with an Illumina TruSeq compatible protocol. The pooled library samples were clustered on an Illumina cBot and sequenced on the Illumina Hiseq2500 platform, using 125 bp single-read v4 chemistry.