Rapid evolution of reproduction without blood feeding in an invasive vector mosquito
Head tissue was disrupted in a Spex Genogrinder 2100 (2 x 1500 RPM for 2 minutes). RNA was extracted using a Zymo Direct-Zol kit (Zymo Research) and mRNA was isolated. Integrity was assessed with an RNA chip (Bioanalyzer 2100). Samples were utilized for paired-end, barcoded, and stranded library construction with the Universal Plus mRNA-Seq protocol (Tecan Genomics). Libraries were sequenced on a single lane.