16S rRNA sequencing for WT mice at baseline (day 0) and 2 weeks after LCWE-injection (Day 14)
Bacteria 16S rRNA gene amplicons were generated using high-fidelity Platinum SuperFi Polymerase (Life Technologies) using the primers 8F-overhang (5 TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGAGAGTTTGATCMTGGCTCAG-3) and R357-overhang (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCTGCTGCCTYCCGTA-3) with the 48Cannealing temperatures and 25 PCR cycles. The underlined regions of the oligonucleotide sequence indicate the Illumina adapter sequence.