sRNA-seq of arabidopsis leaves PsJN Bc infected 3
After adapter ligation to both ends of small RNA, first strand cDNA was synthesized during hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing. The library was checked with Qubit and real-time PCR for quantification and Agilent 2100 Bioanalyzer for size distribution detection. Single-end 50-bp sequencing of small RNA libraries was performed on Illumina NovaSeq 6000 platform.