DAP-seq was performed by purified SMXL7 proteins incubated with prepared Col-0 gDNA library
DAP-seq library preparation: Attach short DNA sequencing adaptors to purified and fragmented gDNA.Target protein expression and purification: Clone the target gene into an expression vector, express in a protein expression system, and purify.Protein-DNA binding: Incubate purified protein with the DNA library, allowing specific binding.Washing and elution: Remove unbound DNA, then elute bound DNA fragments.Library quantification and sequencing: Calculate library concentration and sequence using an Illumina platform.Control samples: Prepare negative-control mock DAP-seq libraries without protein addition for comparative analysis.