16S V3-V4 sequencing of microbiome associated with cultural heritage: wall pink discoloration
DNA amplification was carried out using modified primers with universal tails. Amplified DNA was purified with Thermolabile Exonuclease I (NEB). Unique Dual Indexes (UDI) were linked to the universal tails in a second PCR step. Samples were normalized and the library was loaded on Illumina Miseq instrument and sequenced with a 300 PE strategy. The quality of the reads was checked.