WES of mus musculus: 6-8 weeks male
Whole Exome Sequencing: Ultrasound is used to fragment DNA sequences into random fragments. The fragmented DNA is then sequentially subjected to end repair, 3 end AA addition, and adapter ligation to prepare a DNA library. The constructed library is then hybridized and captured using the Agilent SureSelect Human All Exon V6/V8 liquid-phase kit, followed by magnetic bead adsorption and elution to obtain the target exon sequences. These sequences are then amplified via PCR to form a sequencing library.