GSM9340444: 3_KRAS_noDox_biStellate_run2; Homo sapiens; RNA-Seq
Cells were fixed using the GEM-X Flex Sample Preparation v2 Kit (10x Genomics) according to the manufacturer's protocol (CG000782). Multiplexed libraries were prepared following the 10x Genomics user guide “GEM-X Flex Gene Expression for Multiplexed Samples” (CG000787) using the Chromium X (10x Genomics) and the Chromium Human Transcriptome Probe Set v1.1.0 (10x Genomics). A self-designed customized probe was added, targeting the sequence of the mCherry reporter of the KRASG12D construct (LHS-CCTTGGCACCCGAGAATTCCAATTACGGGGCCGTCGGAGGGGAAGT and RHS-phospho-TGGTGCCGCGCAGCTTCACCTTGTA-ACGCGGTTAGCACGTA-NN-XXXXXXXX-CGGTCCTAGCAA). Two independent sequencing runs were conducted. The first run included all samples of mono- and bi-cultures, while bi-cultures were additionally sequenced in a second run.