GSM8650162: cortex,Olig2,E15; Mus musculus; RNA-Seq
ScRNA-seq were performed on FACS sorted tdT+ cells from the P1 Olig2f/f;IS-reporter mice cortex which were electroporated of pCAG-Cre plasmid at E15.The cortex were immediately submerged in fresh ice-cold Hanks balanced salt solution and  was then cut into pieces and dissociated into a single-cell suspension using a Papain Cell Dissociation Kit according to the manufacturer's instructions. The Chromium dropletbased sequencing platform (10X Genomics) was used to generate scRNA-Seq libraries, following the manufacturer's instructions (manual document part number: CG00052 Rev C). The cDNA libraries were purified, quantified using an Agilent 2100 Bioanalyzer, and sequenced on an Illumina NovaSeq 6000 or Illumina Hiseq4000.