GSM9634905: HUVEC, PBMC and LPS and Abatacept, Donor 3; Homo sapiens; RNA-Seq
PBMCs from the supernatant were harvested. HUVECs were trysinised from the well plates. RNA from each sample was isolated using the RNeasy Mini Kit (Qiagen). RNA libraries for RNA Seq were prepared using QuantSeq 3'mRNA-Seq Library Prep Kit v2 with Unique Dual Indices (UDIs) FWD for Illumina following manufacturor's protocol.