GSM8965346: 35 weeks shell gland breeder # 30 egg in shell gland (active calcification); Gallus gallus; RNA-Seq
RNA was extracted using TRIzol, followong standard company protocol for animal tissue RNA concentration was measured by Qubit 3.0 using RNA BR Assay Kit (Thermo Fisher Scientific). RNA quality was determined with the Agilent Bioanalyzer Agilent 2100 Bioanalyzer (Agilent Technologies) and only samples with an RNA integrity number (RIN) of &gt;7 were further used for RNA-sequencing. mRNA NGS libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) according to the manufacturer's protocol without further modifications.