16S DNA seq of mat microbiome: Great Salt Lake
DNA was extracted using Zymo Quick-DNA Fecal/Soil MicroPrep Kits and amplified using Earth Microbiome Project protocols (515f/806r primer pair for 16S rRNA). Pooled PCR product was purified using the Qiagen Gel Extraction Kit. Sequencing was performed via Illumina Miseq v2 chemistry with paired-end 250 bp reads.