Rongchang pig testis - heat stress
This study aimed to investigate the protective mechanism of lycopene against heat stress-induced reproductive damage in Rongchang pigs. Testis and cauda epididymidis tissues were collected from three groups: control (25C, standard diet), heat stress (HS, 38C for 8h/d for 21d, standard diet), and HS + lycopene (100mg/kg lycopene supplemented in diet). Total RNA was extracted, and cDNA libraries were constructed for Illumina-based RNA-Seq to analyze gene expression profiles.