StudyX_Hs_09_kk+
RNA extraction using Quick-RNATM MiniPrep Plus kit (Zymo Research, Catalog No. R1057); rRNA depletion with NEBNext rRNA depletion kit (New England Biolabs, #E6310L); QC with Nanodrop ONEC (Thermo Scientific), A260/280  1.8, RIN > 5.5; Library preparation using NEBNext Ultra RNA Library Prep Kit (New England Biolabs, #E7530L); Size selection 250600 bp using Agilent 4200 TapeStation (D5000 high sensitivity kit); Sequencing on Illumina HiSeq 2500, 150 bp paired-end.