GSM8965112: RAW cells pamidronate 140uM treatment + 5.25uM GGOH  replicate 2; Mus musculus; RNA-Seq
Total RNA was extracted in triplicate using the Rneasy kit (Qiagen) RNA quality was assessed using an Agilent TapeStation (Agilent, Palo Alto, CA, USA) with RNA tape and RNA concentration was quantified by Qubit 4.0 spectrophotometer. The library was prepared for sequencing from 300 ng RNA of each sample using the Illumina standard total RNA prep ligation with ribo-zero plus library preparation kit (Illumina, San Diego, CA, USA). The quantity and quality of amplified libraries were evaluated using Qubit (Invitrogen, Carlsbad, CA, USA) and Agilent TapeStation high sensitivity D1000 Screen Tape.