GSM8787029: CoSDC CAR-M, rep3; Homo sapiens; RNA-Seq
The cocultures were trypsinized, washed and resuspended in PBS buffer. EGFP+ CoSDC CAR-Ms were sorted by FACS. Cells were washed and resuspended in PBS buffer+0.1% FBS. scRNA-seq library was constructed using the 10X Genomics Chromium system following manufacturer's instructions. In brief, the suspended cells were loaded into Chromium microfluidic chips and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed. The barcoded cDNA was purified and amplified, followed by fragmenting, A-tailing and ligation with adaptors.