title: GSM8542320: MM_MDS_3; Homo sapiens; RNA-Seq
library_construction_protocol: BM cells were isolated from bone marrow aspirates by Ficoll gradient separation of the mononuclear cell fraction. Isolated mononuclear cells were resuspended in 90% fetal bovine serum (FBS) with 10% dimethyl sulfoxide and stored at -80 °C. scRNA-seq was performed on viably thawed BM samples and then loaded onto a microfluidic chip and scRNA-seq libraries generation were performed following the manufacturer's instructions (Singleron GEXSCOPE Single Cell RNAseq Library Kit, Singleron Biotechnologies).