title: GSM9050233: 35FValR; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from liver using TRIzol Reagent according to the manufacturer's protocol (Thermo Fisher Scientific). The concentration and purity of RNA were determined by absorbance at 260/280 nm using Nanodrop (Thermo Fisher Scientific) and samples were diluted to roughly 1000 ng/µl. RNA was submitted to the University of Wisconsin-Madison Biotechnology Center Gene Expression Center (RRID:SCR_017757) & DNA Sequencing Facility (RRID:SCR_017759). Each RNA sample was assayed on an Agilent RNA NanoChip to assess its integrity.  RNA samples that met Illumina's TruSeq Stranded Total RNA Reference Guide (#1000000040499 v00) (Illumina, San Diego, CA) quality criteria were prepared for sequencing following the protocol as recommended using a 250ng total RNA input.  Libraries were multiplexed and sequenced on the NovaSeq6000 sequencer.  Reads were aligned to the mouse (Mus musculus) with genome-build GRCm38.p5 of accession NCBI:GCA_000001635.7 and expected counts were generated with ensembl gene IDs (Zerbino et al., 2018). Illumina's TruSeq Stranded Total RNA Reference Guide (#1000000040499 v00)