title: GSM9342804: Treat_iso15-4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from HCC cells using a commercial RNA extraction kit (Trizol reagent). RNA purity and integrity were verified by NanoDrop and agarose gel electrophoresis, respectively. cDNA libraries were constructed from qualified total RNA. Briefly, mRNA was enriched using oligo(dT) beads, fragmented, and reverse-transcribed into cDNA. After end repair, adapter ligation, and PCR amplification, the libraries were quality-controlled and quantified before sequencing.