title: GSM8853929: Human sickle HSPCs RNA - donor1 edited; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from edited HSPCs 48h following electroporation (ZD7001, Zymo Research). RNA quality and concentrations were assessed by capillary electrophoresis using High Sensitivity RNA reagents with the Fragment Analyzer (Agilent Technologies). Bulk mRNA-seq libraries were prepared by the Imagine Genomic Core Facility (Paris, France) starting from 50ng of total RNA using the NEBNext® Single Cell/Low Input RNA Library Prep Kit according to manufacturer's guidelines.