title: GSM9291930: S2353_Rosa26KO_Young; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Direct-zol RNA Miniprep Plus Kit (Zymo Research). 250 ng of total RNA was used for the construction of sequencing libraries. polyA RNA isolation and library preparation was performed with the Watchmaker mRNA Library Prep Kit (Watchmaker Genomics: 7BK0001 ) with xGEN Stubby adaptors (IDT, Cat: 336338420) and xGEN 10nt UDIs (IDT, Cat: 336338436). Libraries were sequenced with the Element Biosciences AVITI Sequencing platform using the AVITI 2x75 High Output Cloudbreak Freestyle Kit (Element Biosciences, Cat: 860-00015).