title: GSM9343368: 769_TCR; Homo sapiens; OTHER
library_construction_protocol: Fresh brain tissue collected from the operating room in ice cold Hibernate® containing penicillin/streptomycin (120U/ml and 100ug/ml respectively) was transferred to magnesium calcium free Hanks Balanced Salt Solution containing HEPES (20mM), glucose (5mM) and penicillin/streptomycin, and finely minced on ice with spring scissors. Tissue fragments were transferred to RPM1 containing 10% human and HEPES (20mM) and digested overnight at room temperature with Type IV collagenase (~800U/ml) followed by fractionation on a 70%:30% Percoll® (Millipore Sigma, St. Louis MO) step gradient. Two 10x Genomics Chromium single cell gene expression libraries (5' gene expression and TCR V(D)J) were made from each sample and sequenced on a NovaSeq 6000 instrument in a SP flow cell (2x50 bp).