title: GSM9102832: hrp3Δ-1; Schizosaccharomyces pombe; OTHER
library_construction_protocol: Cells were permeabilized in ice-cold yeast permeabilization buffer, and subjected to a run-on reaction with biotin-labeled nucleotides at 30 ºC. RNA was extracted using the Norgen RNA Extraction Kit, treated with NaOH, and cleaned via ethanol precipitation. Final RNA was resuspended in DEPC-treated water for downstream analysis. RNA was processed using streptavidin bead-based purification and ligation of 3' and 5' adapters. Reverse transcription was performed with Maxima H Minus RT, followed by PCR amplification to the logarithmic phase. Libraries were sequenced at the Vienna BioCenter Core Facilities.