title: GSM9560213: Female Down syndrome 2,iPSC [E250027119]; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells and tissues using TRIzol reagent. RNA quality was evaluated using an Agilent 2200 Bioanalyzer, and only samples with an RNA integrity number >8.0 were used for library construction. After rRNA depletion, RNA was fragmented to 150–200 bp, and single‑end cDNA libraries were generated using the Ion Total RNA‑Seq Kit v2.0 (Life Technologies) following reverse transcription, adapter ligation, and PCR enrichment. Library concentration was quantified using an Agilent 2200 Bioanalyzer. Sequencing was performed at NovelBio Corp. Laboratory (Shanghai) on an Ion Proton system using Ion PITM Template OT2 200 Kit v2.0 and Ion PI Sequencing 200 Kit v2.0 (Life Technologies).