title: GSM8891315: Injured tendon, vehicle treated, day 21, biol rep 2; Rattus norvegicus; miRNA-Seq
library_construction_protocol: EVs were isolated using the qEV single 70nm column (Izon Science, New Zealand) according to the manufacturer's instructions. Total RNA was extracted using the miRNeasy Mini Kit (Qiagen, Germany). 5µL total RNA was used as an input for small RNA sequencing library preparation. To each RNA sample 1µL of mind spike-in standards (TAmiRNA, Austria) were added prior to small RNA library preparation using the RealSeq Biofluids library preparation kit (RealSeq Biosciences, US). Adapter-ligated libraries are amplified using barcoded Illumina reverse primers in combination with the Illumina forward primer. Library quality control was performed using DNA1000 Chip (Agilent, United States). An equimolar pool consisting of all sequencing libraries was prepared and sequenced on an Illumina Novaseq with 100 bp single-end reads.