title: GSM9491916: IgG; Mus musculus; OTHER
library_construction_protocol: Cells (4.0 × 10^5 per sample) were processed using the Hyperactive™ In-Situ ChIP Library Prep Kit for Illumina (Vazyme TD901–TD902) following the manufacturer's instructions. Briefly, cells were immobilized on concanavalin A beads, permeabilized with digitonin, and sequentially incubated with anti-EGR1 primary antibody and secondary antibody (Thermo). Hyperactive pA-Tn5 (Vazyme) was then applied to bind antibody-targeted chromatin, followed by in situ tagmentation to generate sequencing-ready DNA fragments. After tagmentation, DNA fragments were PCR-amplified using P5/P7 indexed primers (as provided in the kit). Libraries were assessed for size distribution and quality using an Agilent 2100 Bioanalyzer, quantified per standard Illumina-compatible procedures, and sequenced on an Illumina NovaSeq 6000 platform to generate paired-end 150 bp reads (PE150).