title: GSM9557480: Pcp2-Cre;NfascFx/Fx;AnkGFx/Fx cerebellar hemisphere (Pcp2-DKO), biol rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Cerebellar hemispheres were flash-frozen in liquid N2. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) with on-column DNase digestion according to the manufacturer's instructions. RNA integrity was assessed on an Agilent 4200 TapeStation; all samples had RIN >= 8.2. Poly(A)+ RNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Strand-specific libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit with unique dual indices, pooled equimolarly, and sequenced on an Illumina NovaSeq 6000 S4 flow cell.