title: GSM8504461: HaCaT cells, non-treated control, 12 hours, replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Fungal transcriptome: Total RNA was isolated from C. albicans cells after their coincubation with HaCaT epithelial cells (MOI 10:1) for 4 hours at 37C, in the presence of 5% CO2. MOI = pathogen : host. Total RNA was isolated from cell lines using the RNeasy® Plus Mini Kit, according to the manufacturer's suggestions. Total RNA was isolated from yeast cells with the RiboPure Yeast Kit according to the manufacturer's suggestions. mRNA sequencing library preparation and mRNA sequencing (Illumina) was performed by specialists at Novogene according to their standard library preparation protocols.