title: Transcriptomic profiling of rat epicardial cells under normoxic, hypoxic and reoxygenation conditions
library_construction_protocol: Cell lines from the culture were collected after epicardial conditioned media collection.  Total RNA was extracted from stressed EMCs using QIAzol Lysis Reagent (Qiagen) followed by purification with the Reasy Mini Kit (Qiagen) according to the manufacturerâ€™s instructions. RNA concentration and purity were assessed using a Nanodrop 2000 spectrophotometer (Thermo Scientific), with samples exhibiting A260/A280 ratios between 1.8 and 2.1 and A260/A230 ratios > 2.0 considered suitable for downstream analysis. RNA integrity was further verified using an Agilent 5400 Fragment Analyzer, with RNA integrity numbers (RIN) > 8.0 required for library preparation.  RNA samples were sent to Novogene (UK) for library construction, quality control and sequencing. 