title: GSM9293313: DN2089, Saline, Male; Mus musculus; RNA-Seq
library_construction_protocol: RNA from iBAT was extracted using Tri-Isolate RNA Pure Kit (IBI Scientific, IB47631) following the manufacturer's directions RNA samples were tested for RIN and sequenced by the Genome Center Core at Columbia University Irving Medical Center. Poly-A pull-down method was used to enrich mRNAs from total RNA samples followed by library construction using Illumina TruSeq chemistry. The final PCR step was modified using the KAPA HiFi HotStart Ready Mix.