title: GSM8714561: Liver, Rat 11; Rattus norvegicus; RNA-Seq
library_construction_protocol: Total RNA was isolated from liver using the Direct-Zol RNA Miniprep kit (Zymo Research) and concentrations were determined by Qubit (ThermoFisher Scientific). RNA quality was determined using Bioanalyzer (Beckman Coulter, Inc.), with RIN values > 7. Hepatic RNA samples were processed on Apollo324 for polyA selection prior to library preparation, which was performed using the PrepX mRNA library prep kit for Illumina (Takara Bio USA, Inc.). Sample products were measured for quality and quantity using the Bioanalyzer High Sensitivity DNA chips, with final pooled libraries quantified using the Qubit High Sensitivity kit.