title: Binding of full length p73 (TAp73alpha) and cancer-specific variant (DNp73beta) to genome of melanoma cell line SK-Mel-29
library_construction_protocol: CUT&RUN assays were conducted on SK-Mel-29 melanoma cells transduced with Ad.TAp73α, Ad.DNp73β or the Ad.GFP control, following the manufacturer's protocol (CUT&RUN Assay Kit, Cell Signaling, USA).  Human SK-Mel-29 and SaOS-2 cell lines were maintained at 37 °C in a humidified atmosphere with 5% CO₂ in Dulbecco's modified Eagle's medium (DMEM; PAN-Biotech, Germany), containing pyruvate for SK-Mel-29 and pyruvate-free for SaOS-2. Media were supplemented with 10% fetal calf serum (FCS; PAN-Biotech, Germany), 0.1 mM non-essential amino acids, 50 U/mL penicillin, and 50 µg/mL streptomycin. Adenoviral vectors encoding TAp73α, DNp73β, or GFP have been reported previously (Stiewe et al. 2002; Buhlmann et al. 2008). Infections of SK-Mel-29 were performed at a multiplicity of infection selected to achieve 100% transduction of the target cells. Immunoprecipitation was performed with p73 antibody (1:1000; Thermo Fisher Scientific, USA) libraries were prepared with the DNA Library Prep Kit for Illumina Systems (Cell Signaling, USA) according to the instructions