title: GSM8889930: GFP+CD25+CD4+ cells from Cre control MC38 tumor Mouse2 exp1; Mus musculus; RNA-Seq
library_construction_protocol: MC38 tumor ( Day 12) was taken out and enzymetically digested to make single cell suspension, negatively magnetically purified to mostly get untouched CD4 cells, these cells were then sorted based on CD45+CD90.2+CD4+CD25+GFP+ directly into RNA lysis buffer.(Tim-3 stain was included to confirm effective Knockout of Tim-3 in Tim-3 KO(FLEX4) mouse. SmartSeq cDNA / Nextera XT libraries