title: GSM9604316: SNI, 7DPA, Replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: After euthanasia, tissue distal to the metacarpophalangeal joint was collected, the nail was removed, and samples were stored at −80 degrees celsius. Frozen tissue was mechanically disrupted using rotational shearing blades, homogenized using QIAshredder columns, and total RNA was isolated with DNase treatment using the RNeasy Plus Mini Kit (QIAGEN) according to the manufacturer's instructions. RNA-seq libraries were prepared from 500 ng of total RNA following poly(A) mRNA enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module. Strand-specific cDNA libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit, amplified for nine PCR cycles, and prepared according to the manufacturer's protocol.