title: GSM8597023: Erecya1_p4, bulkRNA; Eretmodus cyanostictus; RNA-Seq
library_construction_protocol: Each intestine was divided into five equally long parts along the anterior-posterior axis, and each section was processed independently. The sections were first mechanically chopped with a razor blade, then homogenized on a FastPrep 24 instrument (MP BioMedicals), and total RNA was extracted using a Zymo Direct-Zol RNA Miniprep kit (Zymo Research), following the manufacturer's protocol. Illumina Truseq library preparation protocol