title: GSM8714403: Ef1a>FLEX[Ascl1] (2w); Mus musculus; RNA-Seq
library_construction_protocol: Following euthanasia, pools of >4 retinas were dissociated using the Worthington Papain Dissociation System (catalog no. LK003150) according to the manufacturer's instructions. Cells were then spun at 4 °C at 400 × g for 10 min and resuspended in neurobasal medium (Gibco no. 21103049), 10% fetal bovine serum (Clontech), B27 (Invitrogen), N2 (Invitrogen), 1 mM l-glutamine (Invitrogen), and 1% penicillin-streptomycin (Invitrogen). The cell suspension was passed through a 35-μm filter and then sorted using a BD FACSAria III cell sorter (BD Bioscience) to retrieve TdT+ cells. Following FACS purification, TdT+ cells were centrifuged at 300g at 4ºC for 5 mins. Supernatant was removed to achieve an approximate concentration of 1000 cells per µl. scRNA-seq libraries were constructed with a targeted cell recovery of 6,000-8,000 cells using the Chromium Next GEM single Cell 3' Reagent kits v3.1 (Dual Index) following the manufacturer's instructions.