title: GSM8991924: MCF10CA1a Exo Replicate 1; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA from sEVs isolated from the conditioned medium of MCF10 cell line series or cultured cells was prepared using TRIzol reagent (Thermo-Fisher), followed by column clean-up using the Monarch RNA Cleanup kit according to the manufacturer's protocol for whole transcriptome isolation (New England Biolabs, T2030S). RNA quality was checked using an Agilent 2100 Bioanalyzer, and concentrations from ThermoFisher's NanoDrop readings were used. Small RNA libraries were constructed using the New England Biolabs (NEB) NEBNext Multiplex Small RNA Library Prep Set for Illumina sequencing and the NEB standard protocol