title: GSM9374655: Tissue cells from the infected joint; Mus musculus; RNA-Seq
library_construction_protocol: Infected knee tissues were homogenized in phosphate-buffered saline (PBS) to generate a single-cell suspension. Cells were stained with Zombie Live/Dead dye and CD45-APC for 30 minutes at 4 °C, followed by washing and resuspension in PBS supplemented with 10% fetal bovine serum (FBS). Live CD45⁺ cells were sorted using a FACSAria cell sorter and subsequently resuspended in PBS containing 10% FBS. Library was prepared using a GEM-X Universal 3' Gene Expression v4 4-plex kit (10x Genomics) according to the manufacturer's instruction.