title: GSM9426402: Tumor; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from tissue and cell samples stored in TRIzol using the Direct-zol RNA kit (Zymo Research, USA), according to the manufacturer's instructions. RNA concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific), and the 260/280 ratio was recorded. RNA integrity was assessed using a Fragment Analyzer system (Agilent, USA). The RNA sequencing library from one patient was prepared using the MGIEasy RNA Library Prep Kit V3.1 (MGI, China) following rRNA depletion with the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme, China). Library circularization was performed using MGIEasy Circularization Module V2.0 (MGI).