title: GSM9372961: P15 PTHrP-mCherry+ scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Mandibular molars were dissected and enzymatically dissociated. Single-cell suspensions were filtered (70 µm) and resuspended in DPBS/1% FBS. PTHrP-mCherry⁺ cells were isolated by FACS (BD FACSAria III; 407/488/561/633 nm; 100-µm nozzle) directly into ice-cold buffer. Single-cell libraries were prepared with the 10x Genomics Chromium Single Cell 3' kit (v3.1) following the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeq 6000.