title: GSM9426233: Primary mouse cardiac fibroblasts, untreated, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from cardiac fibroblasts using Trizol (R411, Vazyme). Enrich mRNA from total RNA using Oligo dT magnetic beads. Following fragmentation, synthesize the first strand of cDNA using random hexamer primers, followed by second-strand cDNA synthesis. After end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification, the library is ready.