title: GSM8964720: anaerobic_rep2; Escherichia coli; RNA-Seq
library_construction_protocol: Total RNA was prepared using a PuroMAG™ Total RNA Purification Kit (Luna Nanotech Inc.), following the vendor's manual, including a 15 minute DNase-treatment at room temperature. RNA quality and concentration were assessed using an Agilent TapeStation and a Nanodrop, respectively. One microgram of total RNA underwent ribosomal RNA removal using RiboRid, and the resulting rRNA-depleted RNA was used to make RNA-Seq libraries with a Kapa Biosystems (Roche) RNA HyperPrep Kit, following the manufacturer's protocol.