title: GSM9159570: psoriasis vulgaris patient 2; Homo sapiens; OTHER
library_construction_protocol: Approximately 2–3 million PBMCs were centrifuged to obtain a cell pellet, which was then processed for DNA extraction using the Monarch Genomic DNA Purification Kit (T3010, New England Biolabs), following the protocol for Cells, Blood, and Tissues. The eluted DNA was quantified using a Qubit 4 Fluorometer (Invitrogen), and its purity was assessed with a Nanophotometer N50 (Implen). DNA fragment size was evaluated using the TapeStation (4150 Agilent), targeting a fragment length of approximately 10–12 kb. After shearing, 2,000 ng of DNA was used as the input for preparing the library with the Ligation Sequencing Kit V14 (SQK-LSK114, Oxford Nanopore Technologies), according to the Reduced Representation Methylation Sequencing (RRMS) protocol.