title: GSM9252148: Lung 2; Mus musculus; RNA-Seq
library_construction_protocol: 1) RNA from CERA cardiomyocytes and CL2 cardiomyocytes was extracted using the Illustra RNAspin Mini Kit (Cytiva). 2) RNA from human left ventricle was extracted using the TRIzol reagent with TissueLyser LT (Qiagen). Extracted RNA was treated with Qiagen RNAse-Free Dnase Set and purified usign the RNase Mini kit (Qiagen). 3) RNA from mouse tissues was extracted using the Aurum™ Total RNA Fatty and Fibrous Tissue Kit (BioRad). 300 ng of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase with an oligo(dT) primer and a template-switch oligo containing a UMI. The resulting cDNA was purified with SPRIselect beads, PCR-amplified with gene-specific and TSO-binding primers, and subjected to bead-based clean-up. Biotinylated amplicons were captured with streptavidin beads, re-amplified, and purified. Final libraries were prepared using the Oxford Nanopore Native Barcoding Kit (SQK-NBD114.24) and sequenced on a PromethION FLO-PRO114M flow cell.