title: GSM8754979: LCM_E13_4; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction was performed with Qiagens RNeasy micro RNA kit. 1ng of RNA was used for cDNA and amplified for 15 cycles. cDNA libraries were made using the protocol Smart-seq2 for sensitive full-length transcriptome profiling in single cells. cDNA libraries were tagmented using home-made Tn5 enzyme and amplified for 11 cycles. Tn5 transposase and tagmentation procedures for massively scaled sequencing projects and Nextera dual indexes (Illumina).