title: Bulk RNA sequencing of mutant MAPT over-expression in neural organoids
library_construction_protocol: Day 90 organoids were collected from culture media, washed in PBS, and then homogenized in RLT buffer with BME. RNA samples were extracted using the QIAGEN RNeasy micro plus kit. Purified RNA samples were submitted to the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core for bulk sequencing. cDNA libraries were prepared with a stranded mRNA (polyA-selected) library preparation kit.