title: GSM9251707: αβ, TH, CRISPR, TCR; Mus musculus; OTHER
library_construction_protocol: The single cell suspensions were stained with CITE-seq antibodies (ADTs and HTOs). Then γδ and αβ T cells were sorted from the thymi and peripheral organs (spleen, liver and lung) of HIS mice transplanted with human HSPCs. Single cells were encapsulated in GEMs ("Gel bead in EMulsion") using the Chromium Next GEM Single Cell 5' Kit v2. After knocking-out (KO) DNTT in the input HSPCs of HIS mice by CRISPR, the single cell suspensions were stained with CITE-seq antibodies (ADTs and HTOs). Then γδ and αβ T cells were sorted from the thymi and spleens of HIS mice transplanted with human HSPCs. Single cells were encapsulated in GEMs using the Chromium Next GEM Single Cell 5' Kit v2 or the GEM-X Universal 5' Gene Expression v3. Separate sc libraries were generated for gene expression (GEX), antibody-derived tags (ADT), and V(D)J TCR according to the user guidelines (CG000330). All steps were carried out using manufacturer-supplied reagents, except for custom primers speciﬁc for TRDC and TRGC constant gene segments (Eurogentec) as described in the paper. For the CRISPR experiment, separate sc libraries were generated for gene expression (GEX), antibody-derived tags (ADT), and V(D)J TCR according to the user guidelines (CG000734). For the CRISPR experiment, all steps were carried out using manufacturer-supplied reagents, except for custom primers speciﬁc for TRDC and TRGC constant gene segments (Eurogentec) as described in the paper.