title: GSM8966068: JHULX33_Empty_CE5; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol reagent (Invitrogen). mRNA was was poly(A) enriched using the NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Biolabs) kit. 50 ng RNA was used for library construction. RNA libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer's protocol.