title: GSM8889946: TGFBa; Homo sapiens; RNA-Seq
library_construction_protocol: Organoids were dissociated to single cells using the Miltenyi Neural Tissue Dissociation kit (P). Singe-cell RNA-seq libraries were generated using Chromium Next GEM Single Cell 3ʹ Kit v3.1 (1000268, 1000120, 1000215, 10x Genomics) according to 10x Genomics user manual CG000315 Rev F. Briefly, cell concentration, viability and percentage of aggregation of single-cell suspensions were quantified on the automatic cell counter Countess 3 FL (AMQAF2000, Thermo Fisher Scientific) with Trypan Blue Stain (T10282, Thermo Fischer Scientific). For each condition, cells were loaded into one lane of a Chromium Chip G (1000120, 10x Genomics) with a target recovery of 1000 - 10000 cells. Single-cell encapsulation, barcoding and library preparation were performed according to the manufacturer's guidelines. Libraries were quantified with Qubit 1X dsDNA HS (Q33231, Thermo Fischer Scientific) and Bioanalyzer High Sensitivity DNA Kit (5067-4626, Agilent) and sequenced on the Illumina NovaSeq 6000 platform.