title: GSM8504563: Day3_RNA_poly_II_CTCF-KO-2; Mus musculus; OTHER
library_construction_protocol: hCD2-Cre, Rosa26-lsl-GFP, and CTCF wild-type or floxed P14 CD8+ T cells were adoptively transferred into CD45-disparate recipients followed by infection with LCMV-Clone 13. At 72 hours (day 3) post-infection, GFP+ P14 cells were sorted and purified for pSer5 RNA polymerase II CUT&RUN analysis. The DNA segments from pSer5 RNAPII Cut&Run were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina HiseqX platform.