title: Moderate nutritional stress reprograms insulin responses to drive enhanced starvation tolerance in Drosophila melanogaster
library_construction_protocol: The samples were collected on day 13 post the training protocol. Day 13 pre-starvation samples are labelled as t0 trained or t0 fed. The samples post 28 hours of starvation are labelled as t28 trained or t28 fed.  RNA extraction was done using Qiagen miRNeasy Mini kit (Cat. No. 1038703). Drosophila melanogaster (https://ftp.ensembl.org/pub/release-112/fasta/drosophila_melanogaster/dna/) reference genome and annotation were downloaded from NCBI. HISAT2 (4) was used to create the reference index and the trimmed & filtered reads were mapped to the indexed genome using HISAT2. Quantification was performed with HTSeq-count (mode, union) (5).