title: GSM9491777: DIPGXIII-SGF29sgC_Rep1_IgG; Homo sapiens; OTHER
library_construction_protocol: SU-DIPGXIII+Cas9 cells were immobilized on ConA beads, permeabilized with 0.01% Digitonin, incubated with primary antibodies, and then cut with pAG-MNase to release DNA fragments as previously described. Libraries were prepared from 3 ng of enriched DNA using the SMARTer ThruPLEX Plasma-seq kit (Takara Bio, Cat. No. R400681) with dual indexes (Takara Bio, Cat. No. DX34752) according to the manufacturer's protocol. Adapter-ligated libraries were amplified for 11 PCR cycles, purified using AMPure XP beads (Beckman Coulter), and evaluated for fragment size distribution on an Agilent 2100 Bioanalyzer. Library molar concentrations were determined by quantitative PCR with the KAPA Library Quantification Kit (Roche) before pooling for sequencing on an Illumina NovaSeq 6000 to generate 150-bp paired-end reads.