title: GSM8598015: EXO1; Homo sapiens; RNA-Seq
library_construction_protocol: Long total RNA-Seq libraries were constructed using the TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero Gold (Illumina, San Diego, CA). The conditioned supernatants of MSCs were collected and centrifuged at 300 ×g for 10 min. The supernatant was centrifuged at 10000 ×g for 30 min and filtered through a sterile 0.22-μm pore filter. The filtered solution was ultracentrifuged at 120,000 ×g for 2 h. Lastly, the supernatant was discarded to obtain the exosome precipitate