title: GSM9374657: 22Rv1-WT-HOTAIR-even-ChIRP-seq-2; Homo sapiens; OTHER
library_construction_protocol: ChIRP-seq: Cells were fixed with glutaraldehyde  for 10 min and nucleus was isolated and sheared to 100-500 bp fragments by sonication, then hybridized with DNA probes. Libraries were prepared using the NEBNext DNA library kit according to the manufacturer's instructions (New England BioLabs), using Illumina novaseq6000. ChIRP-seq libraries were prepared for sequencing using standard Illumina protocols