title: GSM8543640: naïve bladder, immune cell, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Mouse bladder was dissected into small pieces, followed by digestion in RPMI 1640 medium containing 0.34 U/mL Liberase and 10 μg/mL DNase I for 1 h at 37°C. The tissue suspension was filtered through a 70 μm nylon mesh strainer and washed in cold PBS twice.The bladder single-cell suspension was obtained from a pool of 5 naïve 8-10weeks adult female C57BL/6 mice and sorted for CD45+ immune cells at a purity higher than 95% (Beckman MoFlo Astrios EQ). The cell suspension was loaded into Chromium microfluidic chips with 3' chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (HiSeq 2000) according to the manufacturer's instructions.