title: GSM8823489: 2284-Pediatric HGG-H3; Homo sapiens; RNA-Seq
library_construction_protocol: Approximately 10 mg of tumor tissue was allocated for transcriptomic analysis. RNA extraction was performed using the Qiagen RNeasy Lipid Tissue Mini Kit (Catalog #74804). RNA extractions were quantified using RNA Nano Chips (Cat. #5067-1511) on an Agilent 2100 BioAnalyzer. RNA-Seq library preps were constructed using the Illumina TruSeq Stranded mRNA Library Prep kit (Cat #20020595) according to the manufacturers recommendations.  Input was 500ng of total RNA with 10 cycles of PCR amplification.  Final libraries were visualized using High Sensitivity DNA ScreenTape (Agilent, Cat. #5067-5584) on the Agilent TapeStation 2200 instrument.  Quant-It (Invitrogen, Cat. P11495) was used for final concentration determination and libraries were pooled equimolar.  The pool was sequenced single-end on an Illumina HiSeq4000 100 Cycle flowcell with 2% PhiX spike-in.