title: Bulk RNA-seq of immortalized N/TERT keratinocytes stimulated with IFNγ
library_construction_protocol: TRIzol is added directly to the cells in a 6-well plate after overnight stimulation.  Immortalized N/TERT keratinocytes are seeded on a 6-well plate and treated at 80% confluency with 50ng/ml of IFN-gamma (or untreated) overnight. Total RNA extraction was performed using TRIzol reagent. Isopropyl alcohol is added to the the cells homogenized in TRIzol, followed by centrifugation. Library construction was conducted via standard protocol  with Macrogen (South Korea).