title: GSM7838000: Sorted life cells, Primary, patient1, plate KK067; Homo sapiens; RNA-Seq
library_construction_protocol: We have made use of the SORT-seq protocol, which is a partially robotized version of the CEL-seq2 protocol, for scRNA-seq. There is no RNA extraction in the classical sense: the cells within the wells are lysed by heat, and the aformentioned primers will hybridize to the poly-A tail of the mRNAs The following steps take place after primer hybridization: reverse transcription & second-strand synthesis, pooling of wells (one cell per well), in vitro transcription, fragmentation and reverse transcription, PCR amplification of DNA, addition of sequencing adapters: cDNA libraries ready