title: GSM8822328: CB_12MN; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei were isolated from the fresh-frozen cerebellum as previously described (Tsai et al., 2023). NeuN-negative nuclei were separately sorted into 1.5 mL DNA LoBind tubes containing 1 mL of Dijon buffer. A total of 50,000 single nuclei were sorted per sample. Nuclei were then counted, and their concentrations were adjusted to approximately 1 million nuclei/mL. Library preparation was performed using the Chromium Single Cell 3ʹ Library & Gel Bead Kit v3 (10X Genomics). The nuclei/master mix solution was adjusted to target 10,000 nuclei per sample, with an equal distribution of 5,000 nuclei from male and 5,000 from female mice. The prepared samples were loaded onto a standard Chromium Controller (10X Genomics) for processing. Sequencing of the 10X snRNA-seq libraries was conducted by Novogene Co. Inc.