title: GSM8542218: LCMV_CL13_Gp33_IL7; Mus musculus; RNA-Seq
library_construction_protocol: 8 days after the treatment, splenocytes were isolated from the mice and resuspended in ice cold FACS buffer (Ca2+/Mg2+ PBS supplemented with 4% FBS and 2mM EDTA). Splenocytes were stained with antibodies and LCMV-GP33 Tetramer in FACS buffer on ice for 30min. Cells were washed and resuspended in FACS buffer, LCMV-GP33 Tet+ CD8 T cells were sorted using FACSAria II(BD) Library was contructed using manufactures protcol for single cell 5' v2 10x genomics