title: Whole exome sequencing of murine Ink4a/Arf knockout NK cells
library_construction_protocol: Samples of 5 in vitro transformed Ink4a/Arf KO NK cell lines (2 Ink4a/Arf -/- and 3 Ink4a/ARF-/- Rag2-/-) and 12 leukemic Ink4a/Arf KO NK cell lines (6 Ink4a/Arf -/- and 6 Ink4a/Arf-/- Rag2-/- ) were harvested, pelleted, snap-frozen and stored at -80°C. Samples of NK cells isolated from the spleen of healthy/untransformed mice were cultured in the presence of IL2 for 6-8 days and then harvested, pelleted, snap-frozen and stored at -80°C. DNA was isolated from snap frozen cell pellets using the Monarch Genomic DNA Purification Kit (New England Biolabs). Library preparation via enzymatic fragmentation and target enrichment by hybridization was performed using the Twist Bioscience Kit with Twist Mouse Exome panel (Twist Bioscience).