title: GSM8483959: proQ-3XFLAG_lcd_rep3; Vibrio cholerae C6706; RIP-Seq
library_construction_protocol: Cells equivalent to 50 OD600 units were collected and cells were lysed using glass beads. RNA-Co-immunoprecipitation using anti-FLAG-antibody following protein G sepharose, Solution was washed and RNA fraction was isolated with phenol-chloroform-isoamylalcohol. RNA was treated with TurboDNase to remove DNA contaminations. cDNA was synthesized by using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB)  according to the manufacturer's instructions. cDNA libraries were column-based purified and cleaned left-sided using the Agencourt AMPure XP kit (Beckman Coulter Genomics). The quality and quantity of cDNA libraries was measured by Bioanalyzer and Qubit, respectively.