title: GSM8681032: AAA; Mus musculus; RNA-Seq
library_construction_protocol: A total of 5159 cells from three different experimental groups (Naïve: 738, Room Air: 1736, Smoke: 584, and Aneurysm: 2685) were analyzed. Viability for the samples were as follows: Naïve (73%), RA (91%), Aneurysm (94%). Following counting, the appropriate volume for each sample was calculated for a target capture 2000-4000 cells. Samples below the required cell concentration as defined by the user guide (i.e., <400 cells/µl) were pelleted and re-suspended in a reduced volume and counted again using a haemocytometer prior to loading onto the 10x Genomics single-cell-A chip. Libraries were prepared as outlined in the 10x Genomics Single Cell 3′ v2 Reagent Kit user guide Samples were sequenced on a HiSeq 2500 with the following run parameters: Read 1—26 cycles, read 2—98 cycles, index 1—8 cycles. Samples were sequenced to a read depth of ~60,000 reads/cell and run through the 10X Genomics cell ranger platform.