title: GSM8966220: PolyA minus RNA-seq in E14 (TOT2N variant) cells containing stable integration of TetR constuct, replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from cells using the RNeasy Mini Kit (Qiagen). PolyA-minus RNA was isolated using the NEBNext Poly(A) mRNA magnetic isolation module (retaining the unbound fraction). DNA libraries were prepared using the NEB Next Ultra II Directional RNA library preparation kit for Illumina (E7760).