title: GSM8821582: siMETTL3 JURKAT (6h) rep1-resequenced; Homo sapiens; RNA-Seq
library_construction_protocol: Cells from each group and time points were collected and total RNA was harvested with Trizol reagent. NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB #E7770)and NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB #E7760) were used with 400 ng of total RNA for the construction of sequencing libraries. ERCC spike-in were added to total RNA before library construction, following the protocol recommended by ERCC spike-in kit manufacturer. RNA libraries were prepared for sequencing using standard Illumina protocols.