title: GSM9160579: NIH/3T3, WT, Serum starvation 24 h, Xap5; Mus musculus; OTHER
library_construction_protocol: Following a 24-hour starvation period, NIH/3T3 cells were harvested using 0.25% Trypsin-EDTA. Cell viability was assessed using a hemocytometer with Trypan Blue staining. Only cell suspensions with a viability of 90% or greater were carried forward for CUT&Tag library preparation. A total of 100,000 viable cells were used as input for each CUT&Tag reaction. CUT&Tag libraries for sequencing were prepared according to the manufacturer's protocol using Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme).