title: GSM8505826: Tg-infected TS-CTB RNA; Homo sapiens; OTHER
library_construction_protocol: Adherent cells from CTB and EVT samples were trypsinized for 12 minutes with 1:1 TrypLE and PBS. Nonadherent 3D STB were collected from wells. Trypsinized cells were collected by pipetting and centrifuged for 250 x g for 5 minutes. Nuclear extraction was performed according to 10x Genomics Chromium Single Cell Nuclei Isolation protocols. Nuclei viability was tested prior to use in library preparation Library construction was performed according to 10X Genomics Chromium single nuclei multiome ATAC _ Gene Expression protocols