title: GSM8989956: MCF10A, 2.5 µM etoposide; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell suspensions were prepared following the 10x Genomics “Cell Preparation for Single Cell Protocols” guide with a viability of >95%. Single-cell encapsulation, barcoding, cDNA amplification, library preparation, and sequencing were performed by the University of Colorado Anschutz Genomics Shared Resource facility. Cells were encapsulated into droplets using the Chromium Controller, where reverse transcription occurred within individual gel beads-in-emulsion (GEMs). Single-cell RNA sequencing (scRNA-seq) libraries were generated using the 10x Genomics Chromium platform with the 3' mRNA capture kit. Single-cell suspensions were prepared following the 10x Genomics “Cell Preparation for Single Cell Protocols” guide with a viability of >95%. Single-cell encapsulation, barcoding, cDNA amplification, library preparation, and sequencing were performed by the University of Colorado Anschutz Genomics Shared Resource facility. Cells were encapsulated into droplets using the Chromium Controller, where reverse transcription occurred within individual gel beads-in-emulsion (GEMs). Following barcoding and cDNA amplification, sequencing libraries were constructed according to the manufacturer's protocol. Libraries were sequenced on an Illumina NovaSeqX to a depth of 81,810 reads per cell for untreated, 74,214 reads per cell for 2.5µM, 81,254 reads per cell for 10µM, and 76,973 reads per cell for 25µM.