title: GSM8597824: U2OS-CTRL3 ASO-3 days-rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from frozen cell pellets using Qiagen RNeasy Mini kit following manufacturer's instructions (Qiagen, Catalog No. 74106). RNA samples were quantified using Qubit 4.0 Fluorometer (Thermo Fisher) and RNA integrity was checked with RNA Kit on Agilent 5300 Fragment Analyzer (Agilent Technologies). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Catalog No. E7775) for Illumina following manufacturer's instructions RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Catalog No. E7775) for Illumina following manufacturer's instructions