title: RNA-Seq of murine megakaryocyte progenitors (MkPs) from Cdk6 knockout and Cdk6 wild-type CALR-mutant mice
library_construction_protocol: Megakaryocyte progenitors were isolated from mouse bone marrow by means of flow cytometry. The isolated bone marrow was stained for surface markers and the following population was sorted: Lineage (CD11b, Gr-1, Ter119, CD3, CD19) negative, c-Kit positive, Sca-1 negative, CD150 positive and CD41 positive. RNA isolation was performed using RNA-SolvÂ® reagent and manufacturer protocol Libraries were constructed using the Smartseq3 method (Michael Hagemann-Jensen, et al.; Nat Biotechnol. 2020 Jun;38(6):708-714)