title: GSM9372989: KO, female, chow diet, Rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from liver using Qiazol according to the manufacturer's instructions (Qiazol Lysis Reagent, Qiagen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.