title: GSM9131587: AC16, untransfected control, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed with Trizol (Cat# 15596018, Invitrogen) 48 hours post-transfection. Lysates were centrifuged at 13500 × g for 5 minutes and RNA extraction was performed using Direct-zol RNA Kit (Cat# R2050, Zymo PolyA enrichment, library construction, and short-read sequencing were carried out at Novogene USA. Sequencing was performed in paired-end mode with a read length of 150 base pairs and depth of ~20 G per sample on an Illumina NovaSeq platform.