title: GSM8625477: Ctbp1/2dKO vehicle rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the ReliaPrep RNA Miniprep System (Promega #Z6012) according to the manufacturers instructions. In brief samples were mixed with 2-propanol and nucleic acids were bound to a silica membrane by column centrifugation. To remove DNA, DNAseI was added to the membrane and incubated for 15 min before the membrane was washed thrice and dried. RNA was eluted in 30 µl nuclease-free water. 1 ug of RNA was quality controled using the Agilent 2100 Bioanalyzer with the RNA 6000 Nano kit and libraries prepared using Illumina's TruSeq unstranded mRNA Library Preperation kit.