title: GSM9252615: CHATCre-Ribotag_Input_AMPH1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy micro kit (Qiagen) RNA sequencing libraries from Pvalb-RiboTag mouse striatal tissue were prepared using a modified SMART-seq2 protocol (Picelli et al., 2014). Total RNA (6–11 ng) was quantified (Qubit RNA BR Assay) and integrity assessed (Agilent Fragment Analyzer). Reverse transcription was performed with SuperScript II, oligo-dT30VN primers, template-switching oligonucleotides, and betaine. The resulting cDNA was amplified by 8 PCR cycles (KAPA HiFi HotStart), purified (AMPure XP beads), and quality-checked (Bioanalyzer HS DNA Kit). Two hundred nanograms of amplified cDNA was tagmented (Nextera XT Kit) and subjected to 12 cycles of indexed PCR amplification. Libraries were further purified, quantified, and sequenced on an Illumina NovaSeq 6000 system (paired-end 2 × 51 bp) with dual indexing, followed by image analysis and quality scoring using RTA v3.4.4.