title: GSM8890614: TRT3 (J7); Bos taurus; RNA-Seq
library_construction_protocol: According to the manufacturer`s instructions, total RNA was obtained from liver tissue using TRIzol reagent (Molecular Research Center, Cincinnati, Ohio, USA) RNA reverse transcription, library preparation, and RNA sequencing (RNA-seq) were carried out at BGI Co., Ltd. (Shenzhen, China). Briefly, total RNA was enriched for mRNA by poly(A) selection using oligo(dT) magnetic beads, followed by reverse transcription and cDNA synthesis. The resulting double-stranded cDNA underwent end repair, 5'-phosphorylation, and 3'-adenylation to prepare for adapter ligation. The adapter-ligated products were then PCR amplified, denatured, and circularized using bridging primers to create single-stranded circular DNA libraries. Sequencing was performed on the DNBSEQ platform with PE500 (BGI Co., Ltd., Shenzhen, China).