title: GSM8543368: Mov10 Deletion whole brain AGO2 Input, replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Cryopreserved whole brains of WT and Mov10 Deletion P0 pups were ground and UV-crossinliked before lysis using eCLIP lysis mix and sonication. After this AGO2 was immunoprecipitated dephosphorylated with alkaline phosphatase (FastAP, Thermo Fisher) and T4 PNK (NEB) and an RNA adapter was ligated to the 3' ends. RNA adapter ligation, IP-western, reverse transcription, DNA adapter ligation, and PCR amplification were performed as described [PMID27018577].