title: GSM8821031: Wildtype 22º replicate 4; Rickettsia rickettsii str. 'Sheila Smith'; RNA-Seq
library_construction_protocol: Samples were treated with Trizol. RNA was purified using the Direct-zol RNA miniprep Plus kit (Zymo) with the optional DNase I treatment. The RNA Clean and Concentration-100 kit (Zymo) with the optional DNase I treatment was used to purify the RNA. For the genotype study, ribosomal RNA was depleted using the QIAseq FastSelect -rRNA HMR combined with -5S/16S/23S bacteria kit (Qiagen).  Sequencing libraries were generated from the rRNA-depleted RNAs using the Illumina Stranded mRNA Prep, Ligation kit, beginning at the first strand synthesis step (Illumina). For the temperature study, sequencing libraries were generated from total RNA using the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus library preparation kit.