title: GSM9602738: DRAO_Cuboid; Homo sapiens; RNA-Seq
library_construction_protocol: The organoid samples were washed with PBS solution and then resuspended in 0.25% pancreatic enzyme digestion solution at 37 ° C for 5 minutes.    Then digestion was terminated with 10% serum and centrifuged at 500g for 5 min. After removal of the supernatant, the cell particles were re-suspended in a wash buffer (0.04% bovine serum albumin [BSA] in Dulbecco's phosphate buffered saline [DPBS]).   The final cell concentration was adjusted to 1,000 cells/μL (viability≥80%) in all samples by using  Luna Dual Fluorescence Cell Counter Libraries were prepared with the Chromium Single Cell 3′ Library and Gel Bead Kit v3 (10x Genomics) following the manufacturer's protocol. Briefly, single-cell suspensions were loaded into a Chromium Controller to produce Gel Bead-in-Emulsions (GEMs). Following reverse transcription, cDNA amplification, and library construction, sequencing was performed on an Illumina NovaSeq 6000 platform (PE150; Annoroad Gene Technology).