title: GSM8543290: HepAD38 shACSS2-3; Homo sapiens; OTHER
library_construction_protocol: 50000 cell was harvested and incubated with concanavalin A coated magnetic beads for 15 min at RT. Then, bead-bound cells was resuspended and incubated with the appropriate primary antibody (H3K27ac) overnight at 4℃. Afterwards,  secondary antibody was added to this mixture for 30 min at RT. The magnet stand was used to remove unbound antibodies.pA-Tn5 adapter complex was added at this step at RT for 1 h,then,remove unbound pA-Tn5 protein.Next,cells were resuspended in tagmentation buffer at 37℃ for 1 h. After that, immunoprecipitated DNA was saved. Library was sequenced on Illumina novaseq 6000 with PE 150 method