title: GSM8505709: K14_E6-E7 mouse 4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was prepared by homogenization of whole tongue epithelia in TRIzol®  (Invitrogen) followed by phenol:chloroform extraction and RNeasy Mini Kt based column purification with on-column DNase treatment (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification