title: GSM9101307: Foxl1KO-heterozygous rep6; Mus musculus; RNA-Seq
library_construction_protocol: Proximal half of the embryonic small intestine (E15.5) was dissected, minced, and digested enzymatically using collagenase type II and DNase I to obtain single-cell suspensions. Cells were filtered, resuspended in PBS with 0.05% BSA, and live cells were enriched by FACS sorting. Viability and concentration were assessed using Trypan blue staining. The extracted single-cell suspension was immediately processed for library construction. Single-cell RNA-seq libraries were prepared using the 10x Genomics Chromium Single Cell 3' Library & Gel Bead Kit v2 according to the manufacturer's protocol. Approximately 5,000 cells per sample were loaded onto a 10x Genomics Chromium Single Cell Controller. The resulting cDNA libraries were quantified and quality-checked using an Agilent 2100 Bioanalyzer and Qubit Fluorometer, then sequenced on an Illumina NovaSeq 6000 instrument.