title: GSM9103777: SCN9A_2544 treated L6-DRG Rep5; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was isolated via phenol chloroform separation followed by purification of the aqueous/ethanol fraction using an RNeasy 96 plate (Qiagen) according to standard kit protocol, using a vacuum manifold. Library preparation was performed using the NEBNext® Ultra™ II RNA Library Prep Kit using poly(A) enrichment.  Samples were barcoded and multiplexed, and paired end 150 bp (2 x 150bp) sequencing was executed with an Illumina NovaSeq machine.