title: GSM8596803: GS cells, Space-3; Mus musculus; RNA-Seq
library_construction_protocol: GS cells were established from testes of 2-3 day old mice of the DBA/2 strain as previously described (Kanatsu-Shinohara et al., 2003, Biol. Reprod. 69, 216-616). Cells were cryopreserved for 6 months on the ISS or on Earch. Upon return to Earth, GS cells were thawed and total RNA samples were extracted by using TRIzol solution (Invitrogen) and purified by the RNeasy cleanup system (QIAGEN, Valencia, CA). The RNAseq libraries were generated using the  Illumina Stranded mRNA Prep Kit (Illumina, San Diego, CA), according to the manufacturer's instructions.