title: GSM9460179: CE LFD 4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from BAT using TRIzol and chloroform, and purified using a Qiagen RNeasy Mini Kit. To remove contaminating genomic DNA, on-column digestion with Qiagen DNase I was performed as per the manufacturer's instructions. Ribosomal RNA was depleted from total RNA using Biostate AI's proprietary rRNA depletion technology. The remaining RNA was reverse transcribed into cDNA, with each sample barcoded using Biostate AI's Barcode-Integrated Reverse Transcription (BIRT) technology. Sequencing libraries were prepared using TruSeq system dual-index primers (8 bp i5 and i7) and adapters. Libraries were sequenced on the Illumina NovaSeq X Plus platform using paired-end 150 bp reads (151+8+8+151).