title: GSM9426189: BMDMs, non-treatment and IL4-treatment, WT or Smyd5 KO; Mus musculus; RNA-Seq
library_construction_protocol: BMDMs were  lysed for 1 min at 4°C with pre-cold lysis buffer (100 mM Tris-HCl, pH 7.5, 1% SDS, 500mM LiCl, 10mM EDTA, 5mM dithiothreitol). Then, total polyA RNA was extracted by oligodT-beads Using the primer-seq protocols build the Mix RNA-seq library. Briefly, after marking each sample with a barcode, the samples are mixed to form a mix library and then sequenced simultaneously.