title: GSM8568080: 6_FKDL210250685-1a; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspension from spleens and lymph nodes were firstly removed the CD5+, CD19+ and CD11b+ cells via SAV conjugated beads. Then two million live cells from spleen and lymph node were stained with mix1 (CD45.2-PE-cy7, CD11c, CD172α-FITC, MHCII- APC-eFluor780 and XCR1-APC), and all cells from aorta were stained with mix2 (CD45.2-PE-cy7, CD11b-super bright 600, CD11c-PE-cy5.5, MHCII- FITC, CD3- Alexa Fluor 700, CD19- Alexa Fluor 700, Ly6G- Alexa Fluor 700 and XCR1-APC) for 30 minutes at 4 °C. After washing with 1 mL FACS buffer two times, cells from different organs were stained with different anti-CD45 (30-F11) antibodies conjugated with barcode oligos from BD™ Ms Single Cell Sample Multiplexing Kit (BD Bioscience, Cat: 633793). Finally, cells were resuspended with Sytox blue and CD11c+ MHCII+ XCR1+ cells were sorted and subjected to the BD Rhapsody Express system. each group was labelled with sample tag, then built the whole transcriptome analysis (WTA) and sample tag library (TAG) according to the manufacter's instructions (BD Rhapsody™ Single-Cell Analysis System Instrument User Guide (Doc ID 214062))