title: GSM9051951: Bone marrow, l-FA HSCs, rep3; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell    amplification     was     performed     using     SMART-Seq®    HT     Kit(Takara, USA)according to the manufacturer's instructions. Single cells were lysed in buffer containing free  dNTP and  oligo(dT)  oligonucleotides.  The  RNA fragments  were  copied  into  first  strand cDNA using reverse transcriptase and  several templateless C nucleotides. The oligonucleotide primers  were  then  hybridized  with  poly(C)  to  form  Second  strand  cDNA.  cDNA  was  were purified and enriched by PCR The  cDNA  librarys  were  constructed  using  the  KAPA Hyper  Prep  Kit（KAPA,  USA） according to the manufacturer's instructions. Purified libraries were quantified and validated by Qubit® 3.0 Fluorometer and Agilent 2100 bioanalyzer to confirm the insert size and calculate the mole concentration. Cluster was generated by cBot with the library diluted to 10 pM and then were sequenced on the Illumina NovaSeq 6000 (Illumina, USA).