title: GSM9459171: enriched- cells stably expressing cytosolic BS2-TRFLE and pAC-2 treated, biol rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted with QIAGEN Rneasy Plus Mini Kit, followed by Click reaction with biotin and enrichment of biotinylated RNA. 60 µg RNA per sample was used as input. The enriched RNAs for all the samples were subjected to polyA capture and subsequently the DNA library with dual-index adapters was generated and amplified using the mRNA Hyper Prep Kit (Kapa Biosystems, KK8581) following manufacturer's protocol