title: GSM9293953: Single-cell RNA-seq of human iPSC-derived lung organoid; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell suspensions were obtained using enzymatic dissociation, followed by cell counting and viability assessment. Single-cell libraries were constructed according to the manufacturer's protocol for the Chromium Single Cell 3' v4 kit (10x Genomics). In brief, cells and reagents were loaded onto a Chromium Chip to generate Gel Beads-in-emulsion (GEMs), where mRNAs from individual cells were barcoded during reverse transcription. Full-length cDNA was amplified, fragmented, end-repaired, and ligated to sequencing adapters. Indexed sequencing libraries were purified and quality-checked before sequencing on an Illumina platform.