title: GSM9252558: Lama4-/- cortex sample1; Mus musculus; RNA-Seq
library_construction_protocol: Brains were removed, minced into small pieces in ice cold DMEM (Sigma-Aldrich) and digested for 60 min at 37ºC in 30 U/ml papain (LK003153; Worthington Biochemical), 40 µg/ml DNase type IV (LS006331; Worthington Biochemical) and 0.125 mg/ml Liberase TM (5401119001; Sigma-Aldrich) in DMEM. Myelin was removed by centrifugation at 1000 x g for 10 min through 22% BSA. Libraries were prepared according to the manufacturer's instructions using Chromium Single Cell 3' Library & Gel Bead Kit v3 (10X Genomics)