title: GSM9343186: KA092: KP4 - snRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Nuclei from KP4 were isolated using the nuclei isolation kit (CG000505, 10x Genomics) and the manufacturer protocol. Briefly, 1e6 cells were trypsinized and washed once with cold PBS. Cells were then dissociated with lysis buffer and passed through nuclei isolation column. After centrifugation at 16,000 rcf and 4C , nuclei pellet was resuspended in debris removal buffe and washed with cold PBS and 10% BSA . Next, Intact nuclei were resuspended in the resuspension buffer containing 20X Nuclei Buffer, Reducing Agent B and nuclease-free water. Nuclei were profiled for both gene expression and open chromatin using 10x Genomics Chromium Single Cell ATAC-Seq microfluidic cell processing (10x Genomics) and sequenced on an Illumina NovaSeq 6000Multiome data were analyzed using the Signac package