title: GSM9558492: RNA-Seq of Human sorted BM terminal erythroblasts-H4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from sorted BM primary erythroid progenitor cells or terminal erythroblasts. RNA isolation, cDNA library construction, and sequencing were performed at the Annoroad Gene Technology Institute using the Illumina platform with a PE150 strategy. RNA was extracted from sorted bone marrow-derived erythroid progenitor cells and terminal erythroblasts, followed by library preparation and Illumina PE150 sequencing. After quality control with fastp, reads were aligned to the hg38 reference genome using kallisto for expression quantification. Differential expression analysis was performed with DESeq2 (|FC|≥1, p<0.05, adjusted p<0.25). Principal component analysis (PCA) was conducted on normalized counts, and Gene Ontology (GO) enrichment analysis was performed using Metascape (p<0.001) to functionally characterize differentially expressed genes.