title: GSM8853961: skin, mc2rKO, rep2; Danio rerio; ssRNA-seq
library_construction_protocol: A maximum of 30 mg of skin tissue was employed for total RNA extraction from each individual using the RNeasy Mini Kit (QIAGEN GmbH, Germany), in accordance with the manufacturer's instructions. The integrity of the extracted RNA was verified to ensure that the RNA integrity number (RIN) was greater than 8. Strand-specific mRNA libraries were then sequenced on an Illumina NovaSeq X Plus platform at Novogene CO (China), generating paired-end reads of 150 base pairs (bp) in length (23M reads on average).