title: GSM9102445: Multiome, KO Cerebral organoids, 51 day, Rep1,  RNA; Homo sapiens; RNA-Seq
library_construction_protocol: Nuclei were isolated from fresh hiPSC-derived brain organoid tissues (51 days and 74 days) following the 10X Genomics demonstrated protocol for Single Cell Multiome ATAC + Gene Expression sequencing (CG000338). Organoids were triturated in N2/B27 media, filtered through a 70 μm strainer, lysed, and washed. After resuspension in chilled diluted nuclei buffer, nuclei concentrations were determined using a Countess II FL automated cell counter. The sequencing libraries were constructed using the 10X Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression protocol (CG000338). Nnuclei were processed using the 10X Genomics Next GEM Single Cell 3' Gene Expression Library Preparation v3.1 kit with unique dual indexing (UDI), suitable for generating dual modality libraries (gene expression and chromatin accessibility) for sequencing on the Illumina NovaSeq platform