title: GSM8541859: COPD_136; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted after cell lysis by the RNeasy Mini Kit (Qiagen, Venlo, Limburg, Netherlands). To completely remove any possible contaminating DNA, an on-column DNase digestion with the RNase-free DNase set (Qiagen) was performed during total RNA isolation. RNA libraries for RNA-seq were prepared using QUANTSEQ 3' mRNA-seq library prep Kit (LEXOGENE) following manufacturer's protocols.