title: GSM9425934: MBNL1 (4056) eCLIP in K562 cells, IP replicate 2; Homo sapiens; OTHER
library_construction_protocol: Cells were UV crosslinked at 400 mJ/cm2, lysed, and treated with RNase I. Target RBP was immunoprecipitated with specific antibody. RNA was dephosphorylated, ligated to 3' RNA adaptor, and run on SDS-PAGE. Protein-RNA complexes were transferred to nitrocellulose membrane, excised, and treated with proteinase K. RNA was reverse transcribed with AffinityScript (Agilent) and size-selected on TBE-Urea gel. 5' Illumina adaptor was ligated, and cDNA was PCR amplified. Libraries were sequenced on Illumina platforms. Detailed protocol in Van Nostrand et al., 2017, DOI: 10.1007/978-1-4939-7204-3_14.