title: GSM8505177: KO, Model, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Lung tissue from WT_Model and KO_Model mice (n=1, respectively) were harvested. Cell preparation followed by 10x Genomics® Cell Preparation Guide. The cell suspension was loaded into Chromium microfluidic chips with 3' chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (NovaSeq), according to the manufacturer's instructions (Illumina). scRNA-seq library was constructed according to the instruction of the Single Cell 3' Reagent Kit v3.1 (10x Genomics).