title: GSM9251589: S2* cells, IMD, replicate 2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA-seq: Total RNA was extracted with the Zymo Direct-zol RNA miniprep kit STARR-seq: Total RNA was extracted with RNeasy Maxi kit (Qiagen) ATAC-seq: Hemocytes were collected by placing 20-25 anesthetized flies in 0.5 mL tubes with 3 20G needle holes at the bottom. Flies were spun at 6,000xg at 4°C for 1 min into ATAC-seq lysis buffer (Grandi et al. 2022). RNA-seq: Library prep with polyA selection was completed by Azenta (Genewiz) STARR-seq: Library construction followed the protocol from Neumayr et al. 2019. mRNA was isolated with oligo dT beads, Reverse transcription was done with SuperScript® III reverse transcriptase, before junction PCR to isolated STARR-seq specific transcripts ATAC-seq: Lysate was treated with Tn5 for tagmentation, before clean up with Monarch PCR clean up kit (NEB). Barcoding PCR was completed with Illumina primers and qPCR quantification with NEBNext Library Quant Kit (New England Biolabs)