title: ATAC-seq of dihydrotestosterone (DHT) stimulated prostate epithelial cell lines RWPE-1-Ctrl, RWPE-1-AR and prostate cancer cell lines LNCaP-pcDNA3.1 and LNCaP-Arhi
library_construction_protocol: Nuclei isolation was performed using published Omni-ATAC protocol (Corces et al. 2017) with the following modification: cell centrifugation was performed with 700 g for 5 min.  For androgen induction studies, RWPE-1 cells were grown in phenol red-free RPMI-1640 medium with 5% charcoal/dextran-treated (CCS) FBS and 1% glutamine for 3 days. LNCaP were grown in same medium but with 10% charcoal/dextran-treated(CCS) FBS. For androgen induction studies, hormone-deprived cells were treated with 0, 1, 10 or 100 nM of dihydrotestosterone (DHT) for 2 hours before nuclei isolation. Does not apply to ATAC-sequencing. See published Omni-ATAC protocol (Corces et al. 2017) ATAC-library preparation was performed using published Omni-ATAC protocol (Corces et al. 2017) with the following modifications: For Tagmentation reaction conditions were adjusted to 45 min in 37 Â°C at 900 RPM. And for the PCR purification steps, MinElute kit (Qiagen) was used and the library purification was finalized using AMPure Xp beads (Beckman Coulter). 