title: GSM8681011: CS_R1; Mus musculus; RNA-Seq
library_construction_protocol: Abdominal aortae were collected and snap frozen in liquid nitrogen, frozen aortae were digested according to the manufacturers protocol for the RNeasy Mini Kit (Qiagen, catalog#74104) Libraries were prepared with 100ng of total RNA using Stranded Total RNA prep with Ribo-Zero Plus kit (Illumina) with 13 cycles of amplification used. Final cDNA libraries were size validated using Agilent Bioanalyzer or Tapestation and concentration validated by qPCR (Kapa Biosystems/Roche). All libraries were normalized and pooled, denatured with 0.2N NaOH and diluted to a final concentration of 250 pM. Pooled libraries were loaded onto an Illumina Novaseq V1.5 cartridge for cluster generation and sequencing on an Illumina Novaseq 6000 instrument (Illumina) Paired-end 101 bp protocol to achieve approximately 30-40 million reads per sample.