title: GSM9492106: H69EZ-GV HLA positive 2; Homo sapiens; RNA-Seq
library_construction_protocol: For cell line comparison, RNA was isolated directly from duplicate or triplicate samples with the Qiagen (Hilden, Germany) RNeasy isolation kit (Cat# 8028) using on-column DNAse digestion. RNA libraries were prepared from 250 ng total RNA using the Illumina Exome Capture kit per manufacturer's instructions. RNA-sequencing (RNA-seq) was performed per the standard protocols at the Dana-Farber Molecular Biology Core Facilities with Illumina NextSeq 500 instrument. Data quality controls and replicate correlation were evaluated using VIPER software package. RNA libraries were prepared from 250 ng total RNA using the Illumina Exome Capture kit per manufacturer's instructions. RNA-sequencing (RNA-seq) was performed per the standard protocols at the Dana-Farber Molecular Biology Core Facilities with Illumina NextSeq 500 instrument.