title: GSM8597046: Lepatt, scRNAseq; Lepidiolamprologus attenuatus; RNA-Seq
library_construction_protocol: Intestinal samples were flushed, chopped and dissociated at 25ºC for 20 minutes in pre-warmed DMEM/F-12 medium 1X (Fisher Scientific) supplemented with 1 mg / mL of dispase II (Sigma). Suspensions were centrifuged at 500 rcf for 7 minutes at 4ºC, resuspended in 10% Fetal Bovine Serum (FBS) in DMEM/F-12, filtered with a 20 µm cell strainer (CellTrics), centrifuged again with the same conditions, resuspended in 10% FBS in DMEM/F-12, and centrifuged one last time at low speed (200-250 rcf) for 5 minutes at 4ºC. Following instructions from 10X Genomics Chromium Next GEM  Gene Expression 3' v3.1