title: RNAseq of primary human Adipose-derived Mesenchymal Stromal Cells stimulated for 2 days with 20ng/mL IFN-gamma (versus unstimulated control) in presence of 10 nM rapamycin or DMSO (at 1:10000 dilution) as vehicle control
library_construction_protocol: Adipose-derived Mesenchymal Stromal Cells were lysed using RLT lysis buffer from RNAeasy kit  Total RNA was isolated using mini RNeasy kit (Qiagen) with an on-column DNAse I digestion step, according to the manufacturer instructions. The quantity and quality of extracted RNA was evaluated using the NanoDrop One spectrophotometer and the Agilent 2100 BioAnalyzer. Libraries were constructed by the Beijing Genomics Institute (BGI) on a DNBSEQ Eukaryotic Stranded Transcriptome library preparation for Stranded mRNA Library. All RNA integrity numbers were above 9.