title: GSM8855356: NTg, Female, sepsis, 3 mon - 3; Mus musculus; RNA-Seq
library_construction_protocol: Frozen pulverized forebrain tissue was incubated in TRIzol reagent (Invitrogen) followed by phase separation and RNA isolation with ethanol precipitation. RNA was further purified with PureLink RNA Mini kit with on-column DNase treatment (Invitrogen). RNA quality and quantity were measured with the NanoDrop Microvolume spectrophotometers (Thermo) and on the Agilent Bioanalyzer 2100 with the Eurkaryote Total RNA Nano chip. Poly(A) RNA sequencing libraries were prepared following Illumina's TruSeq-stranded mRNA sample preparation protocol. Quality control analysis and quantification of the sequencing library was performed using Agilent Technologies 2100 Bioanalyzer High Sensitivity DNA chip. Paired-end sequencing was performed on Illumina's NoveSeq6000 sequencing system.