title: GSM8542706: HeLa_siNC_untreated_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: For large samples, total RNA was extracted following the standard TRIzol protocol (Invitrogen) for bulk RNA library construction. For small samples, mRNA was isolated with Oligo d(T)25 Magnetic Beads (NEB, S1419S) and directly subjected to Smart-seq2 protocol, as detailed in Plate-based scMAPIT-seq. RNA-seq library was generated using the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, NR604) or Smart-seq2 protocol.