title: HP1, H3-K27me3, and H3-K9me3 Cut & Tag of Het (Cbx1+/+, Cbx3+/+, Cbx5+/-) and TKO (Cbx1-/-, Cbx3-/-, Cbx5-/-) bipotential mouse embryonic liver (BMEL) cells
library_construction_protocol: BMEL cells were trypsinized and permeabilized with NE1 buffer as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. BMEL cells were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% fetal bovine serum (FBS), 1% penicillin-streptomycin, 10 µg/mL insulin, 30 ng/mL IGF-II, and 50 ng/mL EGF, at 37 °C under 5% CO2. Permeabilized cells were treated for Cut & Tag experiments as per https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3. Libraries were PCR amplified for Illumina sequencing using primers described in https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3