title: GSM8990086: DMEM_EV_FL1_unconditioned_medium_2; Homo sapiens; miRNA-Seq
library_construction_protocol: Total EV-RNA was isolated from 400 µl EV fraction with the highest particle concentration using miRNeasy Serum/Plasma Kit (Qiagen) according to the manufacturer's instructions with modifications. DNA libraries were prepared using the MGIEasy smallRNA Library Prep Kit (MGI Technologies) according to the manufacturer's recommendations. In brief, 100 ng of RNA from conditioned or unconditioned medium EVs were used as input. Amplified PCR products were separated using 6 % TBE gel electrophoresis. Different barcoded samples were pooled in equal amount and circularized to generate single stranded DNA libraries (ssDNA). DNA nanoball (DNB) preparation and single-end sequencing (SE50) was carried out as a service at BGI (Shenzhen, China).