title: GSM9344780: AT2 Cell, Bleomycin, D14, replicate 1,  MeRIP-seq-IP; Mus musculus; OTHER
library_construction_protocol: Total RNA was extracted using Trizol reagent.  purified mRNAs were fragmented using an RNA fragmentation kit. A portion of the fragmented mRNA (~4%) was reserved as input control, while the remaining fragmented RNA was incubated with an anti-m6A antibody and subjected to m6A immunoprecipitation (IP) using the EpiMark N6-Methyladenosine Enrichment Kit according to the manufacturer's protocol. Both the IP-purified fragmented mRNA and input RNA were used to construct libraries with the SMARTer Stranded Total RNA-seq Kit v2-Pico Input Mammalian, following the manufacturer's instructions.