title: GSM9342082: experiment 2, scrambled control, sample 1; Homo sapiens; RNA-Seq
library_construction_protocol: For chromatin RNA sequencing, cells were harvested and washed in PBS. Soluble RNA was released by resuspending the cell pellet in ice-cold extraction buffer (described above) with 1U/µl of RNAse inhibitor (New England Biolabs) and incubating on ice for 5 min. Extracted cells were spun down at 1300 g for 4 min and lyzed in qiazol (Qiagen). Cells were shredded using the QIAshredder homogenizer, and separated into organic and aqueous phases after the addition of chloroform and spinning in phase lock gel tubes (Tiangen). Ethanol was added to the aqueous phase containing RNA, and the RNA was collected in RNeasy Mini kit columns (Qiagen). RNA was washed and diluted according to the RNeasy kit protocol. The quality of RNA was verified using nanodrop and by bioanalyser measurements. Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus was used for library preparation. Samples were paired-end sequenced using Illumina NovaSeq6000.