title: GSM9294129: Caco2_IFNl1_noninfected; Homo sapiens; OTHER
library_construction_protocol: DNA was isolated with two consecutive phenol-chloroform extractions and recovered by ethanol precipitation. a PCR reaction using barcoded primers to allow for demultiplexing of the samples after sequencing was performed using the Herculase II Fusion DNA Polymerase (Agilent) and DNA oligos. The resultant PCR products were purified using a QIAquick PCR Purification kit (Qiagen, 28104) and subjected to a second PCR before being purified using Agencourt AMPure XP Beads (Beckman Coulter Life Sciences).