title: GSM8889712: Hippocampus nuclears, WT, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were deeply anesthetized with isoflurane and decapitated. The hippocampus from both hemispheres was dissected and rapidly frozen in liquid nitrogen. The tissue was stored at -80°C until further processing for single-nucleus isolation for snRNA-seq. The Chromium Single Cell 3' Protocol enables to profile 3' digital gene expression of 500 - 10,000 individual cells per sample. Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-scale GEMs (Gel Bead in emulsion) using the Next GEM Technology.Next GEM Technology samples a pool of about 3,500,000 10x Barcodes to separately index each cell's transcriptome and cell surface protein. It does so by partitioning thousands of cells into nanoliter-scale Gel Beads-in-emulsion (GEMs), where all generated DNA molecules share a common 10x Barcode. The poly(dT) primer captures poly-adenylated mRNA and barcoded, full-length cDNA is produced. The Single Cell 3' v3 Gel Beads include two additional primer sequences, that facilitate capture and priming of targets containing interested feature.