title: GSM8651130: striatal nuclei, Dlx5/6-Cre;WT, Mouse ID#475; Mus musculus; ATAC-seq
library_construction_protocol: tissue was homogenized in homogenization buffer (0.32M Sucrose, 5mM CaCl2, 10mM Tris-Cl pH 8, 3mM MgAc2, 0.1% Triton X-100, 0.1mM EDTA), pippetted onto a sucrose cushion, then centrifuged for 90 min at 100,000g. . Nuclei were resuspended in cold lysis buffer (100 µl per 100,000 cells; 10 mM Tris-Cl (pH 7.5), 10 mM NaCl, 3 mM MgCl2, 0.1% (vol/vol) NP-40, 0.1% (vol/vol) Tween-20 and 0.01% (vol/vol) digitonin) and washed in wash buffer (10 mM Tris-Cl (pH 7.5), 10 mM NaCl, 3 mM MgCl2 and 0.1% (vol/vol) Tween-20). Transposition was performed with Tagment DNA TDE1 (Illumina, 15027865). Transposition reactions were cleaned with AMPure XP beads (Beckman, A63880). libraries were generated by PCR with NEBNext High-Fidelity  2× PCR Master Mix (NEB, M0541).