title: GSM8680597: ATAC_UTX_CD8+ Progenitor_3; Mus musculus; ATAC-seq
library_construction_protocol: Sorted cells were performed Omni-ATAC-seq protocol the sorted cells were treated with lysis buffer for 3 min on ice, and the extracted nuclei were resuspended in the transposition mix containing 2.5 ul transposase (Illumina) and incubated at 37oC for 30 min with 1,000 rpm shaker. The products were purified with DNA Clean and Concentrator-5 Kit (Zymo Research) and then amplified with PCR for 11 cycles using barcoding primers provided by previous literature. PCR products were performed size selection using AMPure XP Bead (Beckman coulter) to purify DNA fragments from 150-1,000 bp. The concentration and quality control were measured by Qubit and Tapestation.