title: GSM9050603: 1-month, thalamus, heterozygous replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Brain dissection was performed in the cryostat using frozen brain tissues collected from Srrm2 +/- mutant mice and wild-type littermates. RNA was prepared from microdissected tissues using RNeasy Mini Kit (Qiagen). 200 ng of isolated total RNA from each sample was used for the construction of sequencing libraries. Libraries were prepared using Illumina TruSeq Stranded mRNA Kit (Illumina) following the manufacturer's instructions.