title: GSM8821209: Whole adult Botrylloides diegensis colony including zooids and vasculature.; Botrylloides diegensis; ssRNA-seq
library_construction_protocol: A mature colony, in stage A of blastogenesis with active filtering, newly emerged primary buds, and no signs of secondary buds was processed using the ACME dissociation method García-Castro, H., et. al. (2021). ACME dissociation: A versatile cell fixation-dissociation method for single-cell transcriptomics. Genome Biology, 22(1), 89. https://doi.org/10.1186/s13059-021-02302-5 Library preparation was carried out according to the 10X Genomics protocol for 3' Gene Expression (v3) user guide (10X Genomics CG000183 Rev C). The combined cDNA libraries were sequenced with a length of 150 bp on an Illumina NovaSeq 6000 sequencing platform.