title: GSM8541723: DFY_SAL2; Rattus norvegicus; RNA-Seq
library_construction_protocol: The fresh right lungs of each animal were rinsed and immediately collected in RNAlater™ (Sigma) for RNA preservation, and then they were homogenised in QIAzol® lysis reagent (QIAGEN) with gentleMACS™ Dissociator (Miltenyi Biotec). RNA was extracted from the lung homogenates using the QIAcube robotic workstation with the miRNeasy Mini Kit (QIAGEN), including the DNase digestion protocol. The QuantSeq FWD (Lexogen) kit was used to prepare libraries for RNA-Seq, following manufacturer's protocol.