title: GSM8542599: PC3_rep2, PCHI-C; Homo sapiens; OTHER
library_construction_protocol: PCHiC libraries were prepared from 4-5 x 1e7 cells per library (two biological replicates per cell line using in-nucleus ligation). The HiC libraries were amplified using the SureSelectXT ILM Indexing pre-capture primers (Agilent Technologies) with 8 PCR amplification cycles. Each HiC library (750 ng) was hybridized and captured individually using the SureSelectXT Target Enrichment System reagents and protocol (Agilent Technologies). After library enrichment, a post-capture PCR amplification step was carried out using SureSelectXT ILM Indexing post-capture primers (Agilent Technologies) with 14–16 PCR amplification cycles.