title: GSM8714182: Pt-13_Pri3; Homo sapiens; OTHER
library_construction_protocol: The frozen tissue samples were embedded in OCT, and then cryo-sectioned to a thickness of 10μm using a cryostat. FFPE tissue sections from PDX models were cut to a thickness of 5μm using a microtome. Subsequently, these sections were stained with Hematoxylin and Eosin (H&E) for histological examination. After sectioning onto the Visium slides, sample processing was performed as described in the Visium 10x Genomics protocol for fresh-frozen tissue samples (15 minutes were used for permealization time) or the Visium CytAssist Spatial Gene Expression protocol for FFPE tissue samples. Library preparation for frozen tissue samples was performed as described in  the Visium Spatial Gene Expression Reagent Kit (PN-1000189), Library Construction Kit (PN-1000196), and Dual Index Plate TT Set A (PN-3000431) as described in the Visium protocol for fresh frozen tissue samples (CG000239). FFPE tissue sections for PDX models were prepared following the 6.5 x 6.5 mm Visium CytAssist Spatial Gene Expression protocol (CG000518 for tissue preparation, CG000520 for deparaffinization and H&E staining, and CG000495 for library construction, 10X Genomics).