title: GSM9159948: mouse pancreatic ductal cells, Pten and Brg1 KO, Day21, No6; Mus musculus; RNA-Seq
library_construction_protocol: The pancreases from three HP and three HPB mice were harvested 3 weeks after tamoxifen administration. Each pancreatic sample was digested and pancreatic ductal cells were isolated with cell-specific Dolichos biflorus agglutinin lectin labelling, followed by magnetic bead separation. Total RNA from isolated pancreatic ductal cells was extracted using the RNeasy Micro Kit (Qiagen, Hilden, Germany) RNA libralies for RNA-seq were prepared with SMARTer Ultra Low Input RNA Kit following manufacture's protocols.