title: GSM8990317: Roflumilast 250 nM replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: After treatment, cells were lysed in 100 μL of lysis buffer, and total RNA was isolated using the SV 96 Total RNA Isolation System (Promega) following the manufacturer's instructions. RNA concentration was measured using the Quant-iT™ RiboGreen™ RNA Assay Kit (Invitrogen) and stored at −20°C until further processing. RNA sequencing libraries were prepared and sequenced using the DNBSEQ-G400 platform (BGI protocol).