title: GSM8650657: XX, XO, XY, Del1, Del2, Del3 [scRNA-seq]; Mus musculus; RNA-Seq
library_construction_protocol: Cells were dissociated using trypsin digestion and subsequently washed in incubation buffer (1x PBS, 0.5% BSA,  2mM EDTA) and filtered through a Falcon 40um Cell Strainer (Corning, 352340). Cell death exclusion was performed by adding DAPI (Sigma-Aldrich, D9542). Sorting was performed on a BD FACSAria Fusion (BD Biosciences) and performed by expert operators at the VIB FACS core. Cells were sorted into a 384 well plate. Single-cell RNA-seq libraries were prepared by following a previously published protocol (Hagemann-Jensen et al. 2022), with minor modifications. Library quality was checked using an Agilent Tapestation as well as an Agilent 2100 Bioanalyzer. Library concentration was checked with a Qubit fluorometer (Qubit dsDNA HS Assay). Single-cell RNA-seq libraries were sequenced in paired-end mode (150bp) by BGI Genomics (Shenzhen, China) on a DNBSEQ-G400 sequencer (MGI Tech).