title: GSM9373386: Zebra finch embryo 25 medial hypothalamus E14 control male; Taeniopygia guttata; RNA-Seq
library_construction_protocol: On embryonic day 14, embryos were decapitated and medial hypothalamic punches (1 mm diameter) were collected from 100 Œºm coronal cryosections (10 sections per brain) using a micro-punching technique. Total RNA was extracted using the Zymo Quick-RNA Microprep Kit (cat #R1050). RNA quality and concentration were assessed using NanoDrop spectrophotometry and Agilent 2100 Bioanalyzer. The Genome Centre and Queen Mary University of London prepared mRNA stranded libraries from total RNA using a custom method. Libraries were sequenced on an Illumina NextSeq 500 platform (75 bp paired-end).