title: GSM9293234: INPUT-36; Oryza sativa; ChIP-Seq
library_construction_protocol: A total of 2 g of seedling shoots from transgenic 35S:OsWRKY36-Myc plants were harvested and fixed in a crosslinking solution containing 1% (w/v) formaldehyde under vacuum for 30 min on ice. crosslinking was terminated by treating with 0.125 mM glycine under vacuum for 5 min. ChIP assay was performed using the EpiQuik™ Plant ChIP Kit (Epigentek). The DNA precipitated by ChIP was purified. Purified DNA was amplified by PCR using two primers that could anneal with both ends of the linker.