title: GSM8680474: Control_7_Glandular CD56; Homo sapiens; OTHER
library_construction_protocol: Paraffin-embedded human endometrial biopsies were sectioned and mounted on slides and sent (on dry ice) to the NanoString Technology Access Platform (TAP) for GeoMx digital spatial profiling analysis. Briefly, NanoString GeoMx DSP uses a pool of ~20,000 transcript-specific RNA probes, links each of the probes to a unique, probe specific barcode through a UV cleavable linker. The probe pool was hybridized to target mRNAs on tissue slides (overnight), and slides were then stained using fluorophore-labelled antibodies (“morphology markers”; here CD45, CD56, PanCK, and DNA) and imaged on the GeoMx profiler platform. A total of 3-4 regions of interest (ROIs) were selected per skin tissue sample, and UV light was applied to release and collect the DSP barcodes from selected ROIs. Libraries constructed using NanoString NGS readout protocol where Illumina i5 and i7 dual indexing primers were added to the oligonucleotide tags during PCR to uniquely index each AOI.