title: GSM8543246: st21_dorsal_Dam_rep2; Xenopus laevis; OTHER
library_construction_protocol: For Drosophila DamID, 20-50 third instar larval brains were dissected. For mouse samples, in utero electroporation with DamID plasmids was performed on E13.5 embryos. Electroporated cortex was microdissected. For Xenopus samples, mRNA was injected into stage 11 or stage 21 embryos. All tissue collections were followed by genomic DNA extraction with Qiagen DNAeasy blood and tissue kit and processed for DamID according to previous protocols before library prep. Samples prepared for Illumina sequencing according to a modified TruSeq protocol.