title: GSM8680607: HCC1395_DMSO; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted with DNeasy Blood & Tissue Kit (Qiagen, 69506) for UWB1.289 and MDAMB436 and with ReliaPrep large volume HT gDNA isolation system for COV362 and HCC1395 (Promega, A2751) sgRNA sequences with primers forward: F: 5′- AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNCTTGTGGAAAGGACGAAACACCG-3′, reverse: R: 5′-CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ and purified with Kapa Pure Beads (Roche, KK8802)