title: GSM9232048: CITEseq, RO_676(#1)+RO_681(#2), Pancreas, ADT; Mus musculus; OTHER
library_construction_protocol: CD45+ cells sorted from treated mice, loaded on a GemCode Single-cell Instrument (10x Genomics, Inc., Pleasanton, CA) to generate single-cell GEMs (Gel Bead-in-EMulsions). Single-cell RNA sequencing libraries were prepared using GemCode Single-cell 3ʹ V3.1 Gel Bead and Library Kit (10x Genomics) The cDNA amplification mix was modified to include ADT PCR additive primer (5'-CCTTGGCACCCGAGAATT*C*C-3' where * indicates a phosphonothioate bond) and HTO PCR additive primer (5'-GTGACTGGAGTTCAGACGTGTGC*T*C-3').