title: GSM9050630: B cells, RNA, Spleen 1; Homo sapiens; RNA-Seq
library_construction_protocol: B cells (CD45+CD19+) sorted from the dissociated spleens of EBV infected mice. The quality of the single-cell suspension was evaluated with a hemocytometer under a Leica DM IL LED Fluo microscope, and the quantity was assessed using an automated cell counter (LUNA-FX7, Logos). Approximately 20,000 cells per sample were loaded into the 10X Chromium controller. Library preparation followed the manufacturer's guidelines specified in the Chromium Next GEM Single Cell 5' Reagent Kits User Guide (v2 Chemistry Dual Index) with BCR sequencing. For sequencing, the resulting libraries were processed on an Illumina NovaSeq X platform with a 150 bp paired-end reads configuration. An average depth of approximately 50,000 reads per cell was achieved during sequencing.