title: GSM8544838: HAP1_RACK1KO_VacV_polysome_2; Homo sapiens; OTHER
library_construction_protocol: cell lysates were prepared for sucrose gradient centrifugation and polysome fractionation then RNA was extracted from total, 80S and polysome pooled fractions using RNA extraction kits (QIAGEN, 74104). mRNA samples were prepared using NEB ultraII non-directional kit with polyA selection kit (NEB, #E7775)