title: GSM9102940: ChIP_RNAPIISer2P_ETOP; Homo sapiens; ChIP-Seq
library_construction_protocol: RPE-1 cells were crosslinked with 1% formaldehyde for 10 minutes at 37ºC. Crosslinking reaction was quenched with 0.136 M glycine for 5 minutes at RT. Chromatin was sonicated using Bioruptor Pico (Diagenode) to obtain chromatin fragment size of 250-500 bp. Chromatin associated to each RNAPII Ser2P was obtained by incubating sonicated chromatin with the corresponding antibody. DNA was purified using QIAquick® PCR Purification Kit (QIAGEN). ChIPmentation was carried out using Tn5A Enzyme during the  and libraries were amplified using NEBNext® High-Fidelity 2X PCR Master Mix (NEB). Libraries were purified with Sera-Mag Select Beads (CYTIVA).