title: GSM8680368: EPI2_RUN4_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: Cells from surgical intestinal tissue were defrosted. Tissue was processed to yield high viability single cells, enriched for Epithelial/Stromal/Immune fractions and then cells were tagged with CITE-seq and hashing antibodies. Stromal cells were further subjected to LeviCell enrichment for high quality cells. Cells were pooled and loaded on to the 10X platform for single-cell RNA sequencing. Pooled tagged samples were loaded onto the 10X sc-RNA seq platform, with GEM generation and RNA RT, followed by generation of Gene expression (GEX), Antibody tagged oligo (ADT - with CITE-seq proteins and hashing information and T-cell receptor (TCR) library amplification and indexing, as per protocol CG000186 (Rev A). RNA-Seq paired-end