title: GSM8680610: WT_PF_2; Solanum lycopersicum; RNA-Seq
library_construction_protocol: The plant total RNA was extracted using a TRIzol kit (Invitrogen) according to the manufacturer's instructions. A total amount of 2 μg RNA per sample was used as input material for the RNA sample preparations. RNA library for RNA-seq was prepared as fllows: mRNA was purified from total RNA using polyT and then fragmented into 300~350 bp fragments, the first strand cDNA was reverse-transcribed using fragmented RNA and dNTPs (dATP, dTTP, dCTP and dGTP) and second strand cDNA synthesis was subsequently performed. Remaining overhangs of double-strand cDNA were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, sequencing adaptors were ligated to the cDNA and the library fragments were purified. The template was enriched by PCR, and the PCR product was purified to obtain the final library.the libraries were sequenced on the DNBSEQ-T7 ，150 pair-end.