title: GSM9491802: PLC-M0; Homo sapiens; RNA-Seq
library_construction_protocol: The Isolated mitochondria and whole cells were lysed in TRIzol reagent (Life Technologies). RNA from mitochondria and whole cells was extracted using TRIzol reagent according to the manufacturer's instructions. For total RNA and mitochondrial RNA sequencing, rRNA is removed from the total RNA sample using TruSeq Stranded Total RNA Library Prep Gold (Illumina), and sequencing libraries were generated using NEB Next Ultra Directional RNA Library Prep Kit for Illumina following the manufacturer's recommendations. The libraries were subjected to 150 nt paired-end sequencing with an Illumina Nova seq 6000 system. Each library generated a depth of ~10 G read pairs.