title: GSM9097161: Du145-P, Non-targeting guide, Replica #3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted with NucleoSpin™ RNA Plus Isolation Kit (Macherey Nagel, 740984). Cell Signaling Technology, CUT&RUN Assay Kit #86652  was used and DNA elution was performed according to manufacturer's protocols. Eukaryotic Strand-specific Transcriptome Library Construction & NEBNext® Ultra™ II DNA Library Prep Kit for Illumina