title: GSM8483620: P4-3 (Patient 4, Region 3); Homo sapiens; RNA-Seq
library_construction_protocol: The sections collected in tubes were first washed in PBS to remove the blood, centrifuged, and the tissue pellets were used for subsequent DNA, RNA, and protein extraction with the AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Hilden, Germany) as per the manufacturer's protocol. The quality control and concentration of the RNA samples were measured using the Agilent 2100 Bioanalyzer system. Illumina RiboZero GOLD was used for ribosomal depletion from samples and Illumina TruSeq Stranded total RNA was used for RNA library construction. Multiplex sequencing in 0.25 lane on NovaSeq6000 (NovaSeq Control Software 1.7.5/RTA v3.4.4) with more than 50M reads/sample and a sequencing depth of 2 x 150 pairs, using 'NovaSeqXp' workflow in 'S4' mode flowcell. Out of 16 samples, 4 samples (P1-R2, P4-R2, P2-R3, P2-R4) failed the reception control library quality control step and were not sequenced. RNA-seq, Illumina TruSeq Stranded total RNA, Illumina RiboZero GOLD, -, 1301