title: GSM9160269: Hippocampus, batch 2, excision, sample 2; Mus musculus; RNA-Seq
library_construction_protocol: Before analysis, RNA concentration was quantified at OD260 nm by using a NanoDrop-1000 (Thermo Fisher Scientific, USA), and quality control tests were performed on the Bioanalyzer to ensure there is enough RNA. Aliquots of 10 μg were sent for sequencing at the Australian Genome Research Facility (AGRF), where 1 μg of total RNA was used for sequencing via the Illumina Nova-Seq Control Software (Version 1.6.0.). Real Time Analysis (RTA) (Version 3.4.4) for real-time base calling was used, and it produced a 100bp single end run. The Illumina bcl2fastq (Version 2.20.0.422) pipeline was used to generate the primary sequence data.