title: GSM8503840: striatum TAAR5-WT mice, 10 months, animal 1; Mus musculus; RNA-Seq
library_construction_protocol: Brains were dissected using Rodent brain matrices (1 mm coronal section, cat. # 69808-C, Electron Microscopy Sciences). The slice between AP 2 mm and Bregma was isolated. The cortex was removed from the slice and the remaining striatum was used for RNA isolation. Total RNA was isolated using ExtractRNA (cat. # BC032, Evrogen). RNA quality was checked by capillary electrophoresis using the QIAxcel Advanced program (QIAGEN, Germany). rRNA was removed from total RNA using MGIEasy rRNA Depletion Kit MGIEasy rRNA Depletion Kit according to the manufacturer's recommendations, then RNA was quantified on a Quntus fluorimeter (Promega, USA) and used for preparation of directional libraries using MGIEasy RNA Directional Library Prep Set according to the manufacturer's recommendations. The quality of libraries was checked by capillary electrophoresis using QIAxcel Advanced program (QIAGEN, Germany).