title: GSM8568164: a193es; Homo sapiens; RNA-Seq
library_construction_protocol: The biopsies were immediately transferred and processed in ice-cold phosphate buffered saline. To dissociate the tissue into single cells, the biopsies were chopped with a scalpel and enzymatically digested in multiple rounds using Liberase TH and DNase I at 37°C, with gentle agitation. The resulting cell suspensions were filtered, washed, and prepared for 10x Genomics single-cell RNA sequencing library preparation targeting 5,000-10,000 cells per sample. Library construction was performed following the 10X Genomics 3' v3.1 protocol. In brief, single cells were encapsulated into gel bead-in-emulsions (GEMs) using a chromium controller. PolyA RNA from each cell was reverse transcribed into cDNA. cDNA from all cells were pooled and cleaned up using Dynabeads before being PCR amplified and size selected using AMPure XP beads. Full-length cDNA was then fragmented before adding sequencing primers and indices and doing a final amplication. The final product was again cleaned using AMPure XP beads and sequenced.