title: GSM8504264: ARPE-19 MGME1-/-, inducible mito-ApaLI, Dox induced for 12 hours, rep #1; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 cells were collected and frozen in 10% DMSO in culture media in -80°C before submission to the Integrated Genomics Operation (IGO) core at Memorial Sloan Kettering Cancer Center. Library preparation followed standard ATAC-seq protocol without nuclei isolation (Buenrostro, J.D., Giresi, P.G., Zaba, L.C., Chang, H.Y., and Greenleaf, W.J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature methods 10, 1213-1218. 10.1038/nmeth.2688.).