title: GSM9103782: mutant cells, clone 37, 9 weeks and 4 days of differentiation; Homo sapiens; RNA-Seq
library_construction_protocol: Extended culture (S7 plus 5 weeks) iPSC-β cell aggregates were washed twice with EDTA and dissociated with Accumax (Sigma Aldrich) for 16 min at 37°C. Cells were washed twice in 1% BSA PBS, centrifuged at 300 g for 5 min, filtered using a cell strainer capped tube with 35 µM nylon mesh (Corning, US), counted and adjusted to a 1000 cell/µL concentration. Cell number and viability were evaluated by two researchers using a haemocytometer, after staining with 0.4% Trypan blue (Sigma Aldrich). Between 4910 to 9721 cells were loaded per experiment into the 10× Chromium Single Cell Platform (10X Genomics) using the Next GEM Single Cell 3′ library and Gel Bead Kit (v3.1 chemistry). Cells were processed according to manufacturer's instructions