title: GSM9603782: HepG2, media, Rep2; Homo sapiens; OTHER
library_construction_protocol: RNA was harvested using Rneasy mini kit (Qiagen) from HepG2. RNA-seq library is prepared using KAPA RNA HyperPrep kit (Roche): we used 2ug total RNA and each library was assigned unique i5 and i7 primers to aid in demultiplexing post-sequencing. The library was PCR amplified with the following program (98°C for 45s; 98°C for 15s, 60°C for 30s, 7°2C for 30s and repeat for 11 cycles); 72°C for 1min). PCR reactions from each sample were combined and purified using 0.8X KAPA HyperPure Beads (Roche). Sample concentration post-purification was measured using Qubit™ dsDNA Quantification Assay Kits (Invitrogen).