title: GSM8542428: 1uM STM2457_A; Homo sapiens; OTHER
library_construction_protocol: Epitranscriptomic profiling was conducted using AlidaBio's EpiPlex RNA library preparation kit (#EP100100) using 50 ng of purified, polyA-selected RNA as input. Poly-A selection was performed using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490L). In brief, purified RNA is mixed with spike-in controls, fragmented to ~ 140bp, end-repaired and ligated to the P7 adapter. RNA fragments are captured by specific RNA modification binders. The captured RNA fragments are random primed by bead-anchored P5 adapters. The immobilized P5 adapters comprise the MBC, which is transferred to the cDNA during reverse transcription. PCR amplification concludes the library preparation and paired-end sequencing is used to read the UMI, MBC along with the RNA sequence. A solution control is processed in parallel, skipping the bead exposure