title: RNA-seq of PSM tailbud tissues isolated from wild-type and cytoPFKFB3 mouse embryos cultured in different glucose concentration
library_construction_protocol: Conditional cytoPFKFB3 mice (Miyazawa et al., eLIFE, 2022) were crossed with Hprt-Cre mice, and females were dissected 10 days after a detection of vaginal plug. Isolated PSM tissues were cultured for three hours either in 0.5 mM or 2.0 mM glucose condition. RNA-seq libraries were prepared following the Smart-seq2 described in Picelli et al., Nat Protoc, 2014