title: DNA-seq of genome-wide CRISPR library on Haploid hESCs
library_construction_protocol: Wash cells with PBS x2. Trypsinize (TrypLE Select) the wells with the regular protocol. Spin down the cells after neutralizing the trypsin with a serum-containing medium. count cells freeze pellet gSYNC DNA Extraction Kit (GS100) PCR with Nextera overhangs