title: RNA-seq profiling of Listeria monocytogenes infected macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls
library_construction_protocol: Macrophages lacking Irf2, Irf9, or Irf2 and Irf9, as well as scramble controls were infected with Listeria monocytogenes or uninfected under mock conditions and total RNA was harvested 2 hours post infection. Three independent experiments were performed. Gene knockouts were generated via cas9 guide transduction into cas9-expressing cells.  Total RNA was isolated using TRIzol (Fisher) and Chloroform, and the PureLink RNA Mini Kit (12183018A, Ambion). RNA was then DNase I treated (NEB), and purified using RNA clean and concentrator columns (Zymo). PolyA mRNA enrichment and library preparation was performed by Novogene Corporation Inc.