title: RNA-seq of dihydrotestosterone (DHT) stimulated prostate epithelial cell lines RWPE-1-Ctrl, RWPE-1-AR and prostate cancer cell lines LNCaP-pcDNA3.1 and LNCaP-Arhi
library_construction_protocol: Cells were lyzed directly to cell culture vessels with TRIzol reagent (Thermo Fisher Scientific) For androgen induction studies, RWPE-1 cells were grown in phenol red-free RPMI-1640 medium with 5% charcoal/dextran-treated (CCS) FBS and 1% glutamine for 3 days. LNCaP were grown in same medium but with 10% charcoal/dextran-treated(CCS) FBS. For androgen induction studies, hormone-deprived cells were treated with 0, 1, 10 or 100 nM of dihydrotestosterone (DHT) for 4 hours before cell lysis. TRIzol protocol (Thermo Fisher Scientific) + RNEasy kit (Qiagen) for on-column DNase I treatment with RNase-Free DNase Set (Qiagen) Standard Illumina polyA enrichment libraries, done by Novogene in the seuquencing center in Hong Kong, or in Cambridge Sequencing Center