title: GSM8568166: donor1, CD8+ T cell, 2-week CMV stimulated, Dextramer, V(D)J; Homo sapiens; OTHER
library_construction_protocol: After the cell sorting in Dextramer staining, collected T cell suspension was transferred to laminar wash plate (~80μl in each well) and incubated at room temperature for 30min, followed by washing 5 times with DPBS in Laminar Wash HT2000. Then, the protocol of 10X Genomics Chromium Next GEM Single Cell 5' Kits were used to resuspend T cells into a proper concentration to achieve targeted cell recovery and prepare the V(D)J library for single-cell TCR sequencing. Followed the protocol of 10X Genomics Chromium Next GEM Single Cell 5' Kits