title: GSM9491689: PARCB1, 2W, Rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA from the initial and endpoint cells was isolated using QIAamp DNA Blood Midi Kit (Qiagen, 51183). sgRNA libraries were constructed following a published protocol (Joung J et al., Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening, Nat Protoc., 2017.). In addition, the complete library plasmid was also used as a template to build a sequencing library to estimate the sgRNA distribution in the lentiviral vectors.