title: GSM9343837: CART19 cells, vehicle, RNA-seq 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was purified with the RNeasy Mini Plus Kit (Qiagen). ATAC-seq DNA was prepared using the Active Motif ATAC-Seq Kit following the supplier's recommended protocol. CUT&RUN DNA was obtained using the Active Motif ChIC/CUT&RUN Assay Kit, including antibody binding, MNase digestion, and DNA recovery steps as instructed. RNA-seq libraries were prepared using the Illumina Stranded mRNA Library Prep Kit with Illumina DNA/RNA Unique Dual Indices, following the manufacturer's instructions.ATAC-seq libraries were generated using the Active Motif ATAC-Seq Kit according to the manufacturer's protocol. CUT&RUN DNA libraries were constructed using the Illumina DNA Library Prep Kit following the supplier's recommended procedures.