title: GSM9372900: TTseq measurement of nascent RNA from control KD OSCs after incubuation with 4sU, replicate2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted using NucleoSpin® RNA Kit from Macherey-Nagel, the TT-seq experiment was performed following the protocol in (B. Schwalb et al., 2016) with modifications Libraries were prepared using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB)