title: GSM8542512: HaCaT cells, COL23A1 OE, 6hpi; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the Reliaprep™ RNA Miniprep Systems (Promega, Germany) according to the manufacturer's instructions. 230ng of total RNA per sample were utilized as input for mRNA enrichment procedure with 'NEBNext® Poly(A) mRNA Magnetic Isolation Module' (E7490L; New England Biolabs) followed by stranded cDNA library generation using 'NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina' (E7760L; New England Biolabs). RNA libraries for RNA-seq were prepared using 'NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina' (E7760L; New England Biolabs) following manufacturer's protocol.