title: GSM9101707: brain organoid, Serum-, replicate 2 (RIP); Homo sapiens; RIP-Seq
library_construction_protocol: Cells were lysed without crosslinking. RNA from input samples was extracted using TRIzol. For RIP samples, PHGDH immunoprecipitation was performed, followed by RNA release and extraction using TRIzol. Libraries were prepared using the NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (E6420S) according to the manufacturer's instructions. A total of 10 ng RNA was used for each library preparation.