title: GSM9233071: ATAC-Seq, IMR-90 SV40-T cells, Empty, TGFβ1, rep 1; Homo sapiens; ATAC-seq
library_construction_protocol: A total of 1 × 10⁵ cells were washed with PBS, resuspended in ATAC-seq lysis buffer and incubated with Tagmentation master mix at 37°C for 30 min in a thermomixer at 1,000 rpm. Libraries were prepared using the Active Motif ATAC-seq Kit (53150), indexed with i5 and i7 primers, and size-selected using AMPure XP beads (Beckman Coulter, A63881) at a 0.5–1.3× ratio.