title: GSM9159671: Control_10kPA_R2; Homo sapiens; RNA-Seq
library_construction_protocol: RNeasy Kits for RNA Purification, Qiagen Library preparation for bulk RNA-seq was performed with 500 ng to 1 ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits. Messenger RNA was reverse-transcribed to yield complementary DNA (cDNA) using SuperScript III reverse transcriptase (Life Technologies) and random hexamers. A second-strand reaction was performed to yield ds-cDNA. cDNA was blunt-ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends.