title: GSM9603121: IR + low Sodium butyrate HepG2 replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from HepG2 cells using TRIzol reagent according to the manufacturer's instructions. RNA purity and concentration were assessed using a NanoDrop spectrophotometer, and RNA integrity was evaluated using agarose gel electrophoresis or an Agilent Bioanalyzer. RNA-seq libraries were prepared using a standard Illumina library preparation protocol. Briefly, mRNA was enriched using poly(A) selection, followed by fragmentation, cDNA synthesis, end repair, adapter ligation, and PCR amplification. The libraries were sequenced on an DNBSEQ-T7 platform to generate paired-end reads.