title: GSM8855044: Mouse retina, WT; Mus musculus; RNA-Seq
library_construction_protocol: Mouse retinas were harvested and nucleus extraction was performed using the Miltenyi Nuclei Extraction Buffer (Miltenyi Biotec) according to the manufacturer's guidelines. Upon isolation, the nuclei were counted using trypan blue and a Countess III Automated Cell Counter (Thermo Fisher Scientific, Waltham, MA, USA). SnRNA libraries were prepared using the Chromium Single Cell 3' kit (10X Genomics) and sequenced on an Illumina platform using standard protocols. After obtaining the sequencing data, we used Cell Ranger (v8.0) with default parameters to generate a filtered_feature_bc_matrix.h5 file containing cell barcodes and transcript counts for each sample.