title: GSM8597851: sample1(d3)_CD8_lung_GEX; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh renal tumors were collected in Hank's Balanced Salt Solution (HBSS). After being mechanically dissociated with a scalpel, they were digested with DNAse I (30IU/mL, Roche) and Collagenase D (1mg/mL, Roche) at 37°C for one hour. Primary cells were then isolated by successive filtration on a 70μm Falcon cell strainer (BD Falcon) and a 20μm Falcon cell strainer (BD Falcon) and counted on a Malassez chamber.Cells were then stained with a fixable viability stain FVS 520 (eBioscience), an anti-CD8a labeled with APC fluorophore (Biolegend) and an anti-NKP46 labeled with BV421 (Biolegend) in order to eliminate natural killer cells from the analysis during the cell sorting. RNA libraries were prepared for sequencing using standard Illumina protocols