title: GSM9295441: RNA-Seq wild-type rep 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using Trizol LS followed by a cleanup column (Zymo).  RNA content was quantified using a nanodrop and samples were sent for library preparation and sequencing at Novogene.  For Ribo-seq, corticsal samples were digested with Rnase T1 and monosomes were separated on sucrose gradients.  RNA from monosome fractions were then purified and subjected to library preparation. RNA-Seq libraries were prepared using standard poly(A) mRNA procedures by Novogene.  Ribo-Seq libraries were prepared using the small RNA library preparation kit (NEB) as per the manufacturer's instructions.