title: GSM8990412: Non-senescent BPH epithelial cell, replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA quality was assessed using Nanodrop spectrophotometry and Agilent Bioanalyzer. RNA sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. mRNA was enriched using oligo(dT) beads, fragmented, reverse-transcribed, adapter-ligated, and PCR-amplified. Library quality was checked using qPCR and Bioanalyzer.