title: GSM9160022: U2OS cells siNSUN2 +4OHT; Homo sapiens; OTHER
library_construction_protocol: RNA was extracted by Trizol/chloroform RNA extraction, and further cleaning (to get rid of DNA contamination) with Monarch® Total RNA Miniprep Kit (NEB, T2010S) by following manufacturer's protocol. Sequencing library preparation for RNA004 MinION flow-cell was carried out using the Direct RNA Sequencing Kit (SQK-RNA004) from Oxford Nanopore Technologies (ONT)