title: GSM8855840: ex vivo isolated, cultured Th17 cells, donor 17-3; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted by using a DNeasy Blood & Tissue Kit (QIAGEN). Genomic DNA was sheared by using a Covaris S220 system (Duty Cycle: 5%, Intensity: 3, Cycles per Burst: 200, Time: 70 sec). The DNA sequencing library was generated from sheared DNA using a NEBNext® Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) and the NEBNext® Multiplex Oligos for Illumina® (Methylated Adaptor, Index Primers Set 1). Generated libraries were bisulfite converted by using the EZ DNA Methylation Kit (Zymo Research). Bisulfite converted libraries were PCR amplified by utilizing the KAPA Hot Start Uracil PCR Master Mix (Kapa Biosystems) for 9 cycles. Finally, libraries were sequenced on Illumina HiSeq 2500 using the TruSeq SBS Kit v3 – HS (Illumina).