title: GSM9374486: ACTN1_ACTN1-parent_repAtx1_5.000000uM_A1_techrep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using a custom silica column–based purification kit similar in principle to Qiagen RNA purification systems. 500ng RNA was used for library construction Libraries constructed using Lexogen 3'QuantSeq (Rev) kit with custom first-strand-synthesis primers containing UMIs for PCR deduplication