title: GSM9159921: Epi-iPSC cells, DMSO-treated control day 9 kidney organoid differentiation, biol rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Rneasy Mini Kit (Qiagen). 1μg of total RNA was used for the construction of sequencing libraries. Isolation of Poly(A) mRNA was conducted using the NEBNext Poly(A) Magnetic Isolation Module. Following isolation of poly(a) mRNA, library preparation was conducted using the NEBNext Ultra II RNA Library Prep Kit for Illumina per manufacturer's instructions, individual samples were barcoded using NEBNext Multiplex Oligos for Illumina, and equal sample volumes were pooled prior to sample QC.