title: Transcriptomic characterization of circadian clock gene-mutated human pluripotent stem cell lines
library_construction_protocol: Wild-type and mutant hESCs were maintained in mTeSR™ Plus medium (STEMCELL Technologies, Vancouver, Canada) on culture plates coated with diluted Matrigel (Corning Inc., Corning, NY, USA) in DMEM/F-12 medium (Thermo Fisher Scientific Inc., Waltham, MA, USA). Colonies were passaged every 5–7 days using ReLeSR™ (STEMCELL Technologies) and resuspended in medium supplemented with 10 μM Y-27632 (STEMCELL Technologies).  Total RNA was extracted from hESCs using the miRNeasy Mini Kit (Qiagen), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies). Ribosomal RNA was depleted using the Ribo-Zero™ reagent (Illumina). cDNA libraries were prepared with the TruSeq™ Stranded Total RNA Prep Kit (Illumina) according to the manufacturer's protocol.