title: GSM9602065: 1P0_rep_1; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was extracted from synchronized C. elegans using TRIzol reagent (Invitrogen，USA) following the manufacturer's protocol. PolyA-selected mRNA was used for library preparation with the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB #E7530). mRNA was fragmented, reverse-transcribed, and converted to double-stranded cDNA. After end repair, A-tailing, and adapter ligation (NEB Next Multiplex Oligos), libraries were size-selected (250–300 bp) using AMPure XP beads and PCR-amplified