title: GSM8713734: PANC-1, PKR siRNA 2, rep3, Run1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from PANC-1 cells using TRIzol reagent (Thermo Fisher Scientific). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit from Illumina, the NEBNext Poly(A) mRNA Magnetic Isolation Module, and NEBNext Multiplex Oligos from Illumina (New England Biolabs, Ipswich, MA, USA).