title: GSM9103652: U-2 OS WT, 0.5µM SMG1 inhibitor 11j 2 hours, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolated with TRIzol and chloroform, followed by purification with a spin-column kit (RNA Clean & Concentrator-5, Zymo Research #R1015). polyA-RNA was reverse transcribed with poly-dT barcoded oligos and PCR amplified in the manner of SMARTer cDNA synthesis. This cDNA was multiplexed, cleaned, and prepared via the Oxford Nanopore Ligation Sequencing v14 kit.