title: GSM8891594: BON1_Day0_A; Homo sapiens; OTHER
library_construction_protocol: Both day 0 and day 14 samples were harvested for genomic DNA (gDNA) isolation using DNAeasy blood and tissue kit (Qiagen) in accordance with the manufacturer's protocol. For the amplification procedure, Herculase II Fusion DNA Polymerase (Agilent Technologies) was applied to each sample starting from 5 µg gDNA. First-round forward primer: TTTGCATATACGATACAAGGCTG; First-round reverse primer: TCAAGATCTAGTTACGCCAAGC; Second-round forward primer: TTTCTTGGGTAGTTTGCAGTTTT; Second-round reverse primer: TCAAGATCTAGTTACGCCAAGC. Then the amplified DNA was purified by Select-a-Size DNA Clean & Concentrator kit (Zymo Research) and 6% Novex TBE gel (Thermo). After isolating PCR products from gel using Gel Breaker Tubes and Gel Filters (BioChain), the end-repair and A-tail were added for New England Biolabs (NEB) adapter ligation for library establishment. At last, 2 × KAPA HiFi HotStart mix and NEB dual code barcode were used to enrich adapter-ligated DNA fragments for final library preparation, which was sequenced using an Illumina NovaSeq 6000.