title: GSM9426096: Primary human epidermal KCs (PCS-200-011, ATCC), acne, indole-3-acrylic acid (IAA), rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNAiso Plus reagent (Takara Bio, Shiga, Japan) total RNA was extracted from KCs treated under the same sequential protocol described above (C. acnes exposure for 24 h → medium replacement → metabolite treatment for 24 h). RNA integrity was confirmed before library preparation using a TruSeq RNA Library Prep Kit (Illumina, San Diego, CA, USA). Sequencing was conducted in paired-end 100 bp mode on the NextSeq 550 platform (Macrogen, Seoul, Korea).