title: GSM8544868: NKcells, LDL, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed using the RNeasy Mini Kit (74104, Qiagen, USA) per manufacturers' recommendation. An additional step of DNase I treatment was performed, and cells were washed twice thereafter. Concentration was determined using NanoDrop Spectrophotometer. Libraries were prepared using SMART-Seq V4 (Takara Bio) and Nextera XT kit (Illumina) and were sequenced on Illumina Novaseq for paired-end 100bps using standard Illumina sequencing protocol.