title: GSM9458962: B6 mTSCs; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using a commercial RNA extraction kit (Qiagen) and treated with DNase I (Ambion) to remove residual genomic DNA. 500 ng of total RNA was subjected to ribosomal RNA depletion using the NEBNext rRNA Depletion Kit v2. Strand-specific libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's instructions.