title: GSM8483899: RNAseq-A375-Control-R1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from TSA-treated and untreated A375 cells using the Qiagen RNAeasy plus mini kit (Cat. No. 74134). Cells were lysed and homogenized, spun down in gDNA eliminator columns to remove any genomic DNA. All samples were washed with ethanol and the total RNA was eluted. The quality and quantity of the RNA were quantified using Agilent Bioanalyzer Nano RNA kit. All the libraries used were characterized by a RIN value between 9 and 10. RNA was sent to Oklahoma Medical Research Foundation for library preparation (using an rRNA depletion approach) and sequencing.