title: GSM8542336: SL_1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were deeply anaesthetised with 0.14 g/kg sodium pentobarbital 24 h after the second hit. Acute brain slices were prepared and hippocampi were dissected and dissociated using the papain dissociation system (Worthington, Lakewood, NJ, USA; Cat# LK003153). Cells were loaded onto a 10 X Genomics Chromium chip (10X Genomics, Pleasanton, CA, USA) according to the manufacturer's recommendations. Reverse transcriptions and library preparation were performed using the 10X Genomics Single Cell v2 Kit (Chromium Single Cell 30 Library & Gel Bead Kit v2, 10x Genomics) according to the 10x Genomics protocol. Sequencing was performed on the Illumina NovaSeq 6000 (from Novogene, Beijing, China).