title: THE EFFECT OF MANNOSE ON LPS-INDUCED MACROPHAGES
library_construction_protocol: Mouse monocyte/macrophage-like cell, RAW264.7 cells were cultured in PPMI-1640 medium supplemented with 10% fetal bovine serum。RAW264.7 cells were seeded at 1×106 cells/well in six-well plates in PPMI-1640 medium plus 1% fetal bovine serum overnight for cell synchronization.  The whole RNA was extracted with TRIzolTM Reagent. The isolated RNA is reverse-transcribed and form cDNA libraries