title: Synergistic effects of 5-Fluorouracil in combination with Salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in Colorectal Cancer
library_construction_protocol: Each group of 2x106 cells was used for eukaryotic mRNA sequencing Each group of 2x106 cells was used for eukaryotic mRNA sequencing, total RNA was provided from the cell samples, RNA concentration and purity were detected using Nanodrop2000, and then a whole-genome kit was used for RNA library construction. Use Oligo (dT) magnetic beads with polyA for base complementary pairing to isolate and enrich the mRNA. Then add fragmentation buffer to randomly fragment the mRNA. 300bp small fragments are sorted by magnetic beads and reverse transcribed into double fragments using a reverse transcription kit. Strand cDNA, fill it with End Repair Mix and connect to the adapter. After the above processing, the Illumina platform: i) library enrichment, PCR amplification cycles; ii) 2% agarose recovery; iii) TBS380 (Picogreen) machine; iv) cBot bridge PCR amplification to generate clusters; v) PE library, read length 2Ã—150bp, sequenced. 