title: GSM9101491: notag_ip_replicate1; Caenorhabditis elegans; RIP-Seq
library_construction_protocol: C. elegans were crosslinked with 1.8% formaldehyde and sonicated. Lysates were centrifuged, and the supernatant was incubated with Anti-FLAG® M2 magnetic beads (Millipore Sigma, M8823). Bound RNAs were reverse crosslinked and extracted using TRIzol. Libraries were prepared following a previously described protocol (Guo et al., 2024, Mol. Cell, PMC10997485).