title: GSM9376190: human.lung.CAF.1.uM.TEADi.2; Homo sapiens; RNA-Seq
library_construction_protocol: After 48 hours of treatment, cells were harvested using 1X Gibco TrypLE (Thermo Fisher Scientific) and centrifuged at 5000 rpm for 5 minutes to collect cell pellets. Total RNA was isolated using the QIAGEN RNeasy Plus Mini Kit (QIAGEN) following the manufacturer's instructions. RNA quality was assessed using High Sensitivity RNA ScreenTape on TapeStation 4200 (Agilent Technologies) and quantified using the Quant-iT RiboGreen RNA Kit (Thermo Fisher Scientific) on a Victor X2 Multilabel Microplate Reader. Sequencing libraries were generated using the Illumina Stranded mRNA kit (Illumina) with an input of 100-1000 nanograms of total RNA. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The average library size was determined using D5000 ScreenTape on TapeStation 4200 (Agilent Technologies). Libraries were pooled and sequenced on NovaSeq X Plus (Illumina) to generate 30 million single-end 50-base pair reads for each sample.