title: GSM8679744: RNA-seq, K562, Donor + B2M RNP, Mock; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq, T7 RNA was extracted from IST reactions by TRIzol LS and column purification. For Superb-seq, combinatorially barcoded cDNA was generated by Evercode WT Mini kit (Parse Biosciences) and extracted from fixed cells by SPRI beads according to Evercode kit instructions. RNA-seq library was prepared by QIAseq UPXome kit (QIAGEN). Single-cell Superb-seq library was prepared by Evercode WT Mini kit (Parse Biosciences). Both libraries were prepared according to kit instructions.