title: GRAS-Di genotyping
library_construction_protocol: Genomic DNA extracted from mouse tissues was subjected to GRAS-Di (Genotyping by Random Amplicon Sequencing - Direct) library preparation according to the protocol provided by Toyota Motor Corporation and described by Hosoya et al. (Mol Ecol Resour 2019). Random primers (64 types) were used in the first PCR, followed by a second PCR for index tagging. Libraries were sequenced on the MGI DNBSEQ-G400RS platform to generate 238 bp paired-end reads.