title: GSM9052586: Liver, CCl4, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Liver tissues from Brd4fl/fl, Brd4LSECKO mice were perfused according to the protocol of LSEC isolation to obtain single cell suspension. Then cells were centrifuged at 50 g for 5 minutes to remove hepatocytes. The collected suspension was centrifuged at 300 g for 10 minutes to obtain cell pellet, which was washed twice and resuspended in cold PBS with 0.04% BSA. Then cells were counted using a hemocytometer. After counting the cells, 10,000 cells were decided as the appropriate target volume for each sample. The samples were then loaded onto the 10x Genomics single-cell-A chip. After single-cell suspension was prepared, the following steps were execuded following the Chromium Single Cell 3' Reagent v3 user guide. In brief, droplets were generated with the single cells and barcoded beads, after which cDNA synthesis was performed. The droplets were broken, then cDNA was amplified and libraries were constructed. Finally, the single-cell libraries were sequenced on the Illumina platform.