title: GSM8650732: IL1R_KO2_brain_candida_8h; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from whole mouse brain (Il1r-/- and Il1r+/- mice, 8h after infection with 105 CFU C. albicans) using RNeasy plus micro kit (Qiagen) according to the manufacturer's protocol. Libraries were prepared using SMART-Seq RNA Stranded sample kit (Takara Bio) and sequenced by NovaSeq X Plus (Illumina). Sequencing was done using paired-end 150bp reads with a final sequencing depth of 25M paired-end reads per sample on average.