title: GSM9344433: CAR22/41BB/CD3z-short linker, D7 chronic stimulation, rep 1; Homo sapiens; OTHER
library_construction_protocol: CUT&RUN sample preparation was performed using the CUTANA CUT&RUN kit (Epicypher 14-1048). Briefly, 500,000 nuclei per reaction were extracted from thawed CAR T cells using nuclei extraction buffer (EpiCypher 21-1026). 1ug of antibody (control IgG, H3K4Me3 or cJUN) was used to pull down fragments. Sequencing libraries were prepared using the CUTANA Library Prep Kit (Epicypher 14-1001 & 14-1002) and following manufacturer instructions. CUT&RUN sample preparation was performed using the CUTANA CUT&RUN kit (Epicypher 14-1048). Briefly, 500,000 nuclei per reaction were extracted from thawed CAR T cells using nuclei extraction buffer (EpiCypher 21-1026). 1ug of antibody (control IgG, H3K4Me3 or cJUN) was used to pull down fragments. Sequencing libraries were prepared using the CUTANA Library Prep Kit (Epicypher 14-1001 & 14-1002) and following manufacturer instructions.