title: GSM9458998: ECEV_RNA1; Homo sapiens; ncRNA-Seq
library_construction_protocol: RNA was harvested using Trizol and purified with an RNA Clean & Concentrator-25 kit (Zymo, Tustin, CA, USA). RNA samples were submitted to Novogene (Novogene Corporation Ince., Sacramento, CA, USA) for quality control, library preparation, sequencing, and quantitation as per the in-house standard. The library preparation, genome mapping, and raw gene counts for mRNA-sequencing analysis were performed by Novogene (Novogene America). The following methods were those provided by Novogene. Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18~40 bp were ready for sequencing on Illumina sequencing with SE50. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.