title: GSM8543876: Tree Shrew Primary Neurons, PBS-1, 24h, rep4; Tupaia belangeri; miRNA-Seq
library_construction_protocol: Agarose gel electrophoresis was used to assess the degree of RNA degradation and potential contamination. The purity of the RNA (OD260/280 ratio) was measured using Nanodrop, while the RNA concentration was precisely quantified with Qubit. The integrity of the RNA was accurately evaluated using the Agilent 2100 Bioanalyzer. Using the Smal RNA Sample Prep Kit, a library was constructed by leveraging the unique structures of the 3' and 5' ends of small RNA (with a complete phosphate group at the 5' end and a hydroxyl group at the 3' end). Starting with B RNA as the initial sample, adapters were directly added to both ends of the small RNA, followed by reverse transcription to synthesize DNA. The resulting DNA was then amplified by PCR, and the target DNA fragments were separated by PAGE gel electrophoresis. The desired cDNA library was obtained by excising and recovering the gel fragments.