title: GSM8890280: UMUC5_4h_Veh_1; Homo sapiens; OTHER
library_construction_protocol: Cells were lysed directly in TRIzol followed by isolation of total RNA. Bru-labeled RNA was immunocaptured using anti-BrdU antibodies (BD Biosciences). Strand-specific libraries were prepared from a modified protocol using the TruSeq Kit (Illumina) as previously described (Paulsen et al., 2014; Paulsen et al., 2013). This protocol used single-index barcoded ligation adapters and universal primers for PCR. Ribosomal RNA was reduced via QiaSeq FastSelect (Qiagen) prior to first strand synthesis. These strand-specific libraries were made using a universal ligation adapter and dual-index, barcoded primers for PCR. Size selection for all libraries was via agarose gel excision.