title: RNA-seq of Bordetella pertussis WT BPSM and crpH knockout strains cultivated in high and low aeration
library_construction_protocol: Bacterial suspensions (8 ml) were collected at mid-exponential phase and centrifuged at 4,000 rpm for 15 minutes at 4Â°C after adding 2 mL of a phenol:ethanol solution (5:95, v/v). The bacteria were grown with or without agitation in SS medium supplemented with 20 mM MgSO4. Total RNA was extracted using Tri-Reagent, followed by two consecutive DNase I treatments, each followed by purification using AMPure XP beads to remove genomic DNA and contaminating solvents. Strand-specific RNA-seq libraries were prepared with the QIAseq Stranded RNA Library Kit according to the manufacturer's instructions.