title: GSM9231493: static3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction of hiPSC-derived organoids were performed with TRIzol reagent, and samples were kept at -80 °C until further processing. Following the manufacturer's instructions, RNA was isolated using the Macherey-Nagel Nucleospin RNA isolation kit. Library preparation is made by SZAOMİCS using the Illumina Stranded total RNA Prep (Ribo-Zero Plus) Kit. The total-RNA samples are depleted from abundant transcripts, such as ribosomal RNA, using Ribo-Zero Plus depletion reagents. The samples are then chemically fragmented and turned into cDNA using reverse transcriptase, while subsequent ligation and amplification steps add the Illumina index-adapters for clustering and sequencing.