title: GSM9101997: HT1080_6KO_1; Homo sapiens; RNA-Seq
library_construction_protocol: 3x10^6 cells were pelleted, resuspended in MEM with 5% glycerol, and frozen at -80 °C. Cells were shipped on dry ice, and total RNA extracted by Creative Genomics using a commercially available kit. Quality of RNA was verified using RNA Screen Tape (Agilent). RNA was rRNA-depleted using the Ribo-Zero plus kit (Illumina). Libraries were prepared using the PE150 platform (Illumina) following the manufacturer's instructions.