title: GSM9343667: DKC1_KD_C6_biol rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from the SW620 cells using TRIzol reagent (Ambion, Life technologies) as per the instructions provided by the manufacturer. Quality of the isolated RNA was assessed using 2100 Bioanalyzer (Agilent Technologies). Library was prepared using Illumina TruSeq Stranded mRNA Library prep kit (Reference Guide # 1000000040498 v00) as per manufacturer's instructions. Paired-end sequencing was performed at Macrogen Inc. (South Korea) on NovaSeq 6000 platform with a 150bp read length and more than 25M reads recorded per sample.