title: GSM8855661: cortex, methylphenidate 2; Mus musculus; RNA-Seq
library_construction_protocol: Cerebral cortex collected from 6-week-old F2 mice was flash-frozen in liquid nitrogen and stored at -80℃ until total RNA was extracted. Total RNA was isolated from the tissue using RNeasy Mini Kit (QIAGEN Inc., CA USA)" Poly(A) RNA was prepared by the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, Ipswich, MA, USA). Sequencing libraries were prepared by the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA, USA).