title: GSM8754188: Rv1, control, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Two biological replicates (1x105 living cells per replicate) were used for sample preparation, following the ATAC-seq kit manual (Active Motif). In brief, cell nuclei were extracted using ATAC lysis buffer and incubated with 50 μl of Tagmentation Master Mix at 37° C for 30 minutes in a thermomixer. The transposed DNA was purified with spin column. we prepared sequencing library using Nextera index kit. The library was purified with double-sided SPRI beads and library fragment size was evaluated using an Agilent Bioanalyzer.