title: GSM8989852: SSM2,24H,sample2; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell RNA sequencing was performed at two critical timepoints: 24 hours post primary immunization and 7 days post-secondary immunization. Pulmonary tissues were harvested and mechanically dissociated to prepare single-cell suspensions. Aliquots containing approximately 10,000 viable cells per mouse were processed through a Chromium Single Cell Controller (10X Genomics) to generate gel bead-in-emulsions (GEMs). cDNA libraries were prepared using the Chromium Single Cell 5' Kit (v2, PN-1000165) and Single Cell V(D)J Enrichment Kit (PN-1000215), following standardized protocols for reverse transcription, cDNA amplification, and library construction. Final sequencing was conducted on an Illumina NovaSeq 6000 platform through Novogene's commercial sequencing services.