title: GSM8597765: human OFC, OF69, CONTROL; Homo sapiens; RNA-Seq
library_construction_protocol: human: Total RNA was extracted using Invitrogen ToTally RNA Kit (Life Technologies); rat: RNA extraction from the isolated tissue was conducted by TRIzol and isopropanol precipitation human: RNA-seq libraries were prepared from 1 ug of total RNA using Illumina's TruSeq RNA library preparation kit V2; rat: Using the SMART-Seq v4 Ultra Low Input RNA Kit (Clontech), reverse transcription was performed on polyadenylated transcripts, along with second-strand synthesis of full-length cDNA and amplification of the product (Azenta Life Sciences, Chelmsford, MA).