title: GSM9232429: Cohouse old 4; Mus musculus; OTHER
library_construction_protocol: tool DNA was purified using DNeasy PowerSoil Pro Kit (Qiagen, Cat. No. 47014) according to manufacturer's instructions. DNA was amplified using the KAPA Hifi HotStart ReadyMix (Roche, KK2602) and the F27/R338 primer-pair targeting the V1V2 region of the 16S gene (F27: 5'- AGAGTTTGATCCTGGCTCAG-3', R338: 5'- TGCTGCCTCCCGTAGGAGT-3'). After PCR amplification, the pooled library underwent bead- based size selection using AMPure XP beads (Beckman Coulter, A63881) and was sequenced using 500 bp paired-end sequencing (Illumina MiSeq).