title: Precision targeting of C3+ reactive astrocyte subpopulation with endogenous ADAR in iPSC-derived model
library_construction_protocol: Astrocytes derived from iPSCs were detached from MatriGel coated plate with Accutase. Cells were then rinsed with PBS and incubated in Trizol (Thermo Fisher 15596026) for 10 minutes at room temperature. RNA was extracted using a Direct-zol RNA miniprep kit (Zymo R2050) according to the manufacturerâ€™s instruction. This process was repeated two additional times from independent iPSC differentiations (biological n=3).  Raw sequencing reads were obtained for the 9paired-end samples.FASTQ reads were mapped to human genome (hg19) by HISAT2 (version 2.1.0)