title: GSM9375849: VOA-4627-Replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's protocol, which included on-column DNase treatment to remove residual genomic DNA. 1ug of total RNA was used for the construction of sequencing libraries RNA libraries were prepared by the Molecular Genomics Core (Peter MacCallum Cancer Centre, Australia) using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs), incorporating rRNA depletion to enhance the detection of low-abundance transcripts.