title: GSM9460018: CIr-24h-3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cells using TRIzol reagent (Invitrogen, USA) according to the manufacturer's instructions. RNA concentration and purity were assessed using a NanoDrop spectrophotometer (Thermo Scientific, USA). For each sample, 1.0 μg of total RNA was used for RNA-seq library construction. RNA-seq libraries were constructed from 1.0 μg of total RNA using a standard poly(A) mRNA enrichment protocol following the manufacturer's instructions.