title: GSM8965865: RP_C3_MYC; Mus musculus; OTHER
library_construction_protocol: For ChIP-Seq formaldehyde cross-linking was used, followed by Micrococcal nuclease treatment, sonication, and Chromatin IP with specific or control antibodies. CUT&RUN was performed according to published methods using pA-MNase as secondary reagent. Yeast DNA was spiked into the samples after DNA extraction to normalize signal between samples. Cells were processed on an automated system at the Fred Hutch Cancer Center to carry out MNase cleavage, DNA extraction, and library construction. for ChIP-Seq, libraries were prepared using the Kapa system, 25x25 paired-end sequencing was performed on an Illumina HiSeq 2500 instrument. For CUT&RUN, cells were processed on an automated system at the Fred Hutch Cancer Center using KAPA DNA polymerase method for library preparation.