title: GSM8598119: MM468_VPRFOSL1_gRNA3; Homo sapiens; OTHER
library_construction_protocol: 1x10^6 cells were blocked for 10min at 4°C with 5uL of human Trustain FcX (Biolegend, Ref: 422301) and stained for 30min at 4°C with 1ug of TotalSeq A antibodies (Biolegend, 1 antibody per sample for MDA-MB-468 GOF VPR control, VPR FOSL1 gRNA2, VPR FOSL1 gRNA3, HA control and HA FOSL1). After washes, cells were filtered and counted. A pool of 6 stained samples was prepared by mixing of 25 000 cells per sample (finale concentration of the pool: 2000 cells/uL) Approximately 16,000 cells were loaded on a Chromium Single Cell Controller Instrument (Chromium Single Cell 3ʹv3, 10X Genomics, Ref: PN-1000075) according to the manufacturer's instructions. Samples and libraries were prepared according to the manufacturer's instructions. Libraries were sequenced on a NovaSeq 6000 (Illumina) in PE 28-8-91 with a coverage of 50,000 reads/cell.