title: GSM8484160: total_lung_control_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using an RNA purification kit (Qiagen) RNA quality was assessed using the Agilent 2100 Bioanalyzer. Total RNA (500 ng) was prepared for sequencing using polydT-mediated cDNA synthesis according to the manufacturer´s (Illumina) instructions. Subsequent library preparation was performed using the NEBNext RNA library preparation kit for Illumina. Library quality was assessed using the Fragment Analyzer (AATI) followed by library quantification (Illumina library quantification kit). Sequencing was carried out on a NovaSeq 6000 platform (Illumina).