title: GSM8855185: YT cells,OE226,1; Homo sapiens; RNA-Seq
library_construction_protocol: Lyse the sample in TRIzol or lysis buffer, add chloroform (if using TRIzol), and centrifuge to separate phases.Transfer the RNA-containing aqueous phase, precipitate RNA with isopropanol, wash with ethanol, and resuspend in RNase-free water. Convert extracted RNA into cDNA, fragment it, add adapters, and amplify to create a sequencing-ready library.