title: GSM8542244: DRG, Male, 1W, IL, rep 1; Rattus norvegicus; RNA-Seq
library_construction_protocol: Arturus PicoPure kit (with lysis at 30Hz for 2 mins in a Retsch Bead Mill) RNA quality was checked using a 2100 Bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies). The RIN for all samples was ≥ 7,5. DNA libraries suitable for sequencing were prepared from 300-500 ng of total RNA with oligo-dT capture beads for poly-A-mRNA enrichment using the TruSeq Stranded mRNA Library Preparation Kit (Illumina) according to manufacturer's instructions (1/2 volume). After 13-15 cycles of PCR amplification, the size distribution of the barcoded DNA libraries was estimated ~310 bp by electrophoresis on Agilent DNA 1000 Bioanalyzer microfluidic chips. Sequencing of pooled libraries, spiked with 1% PhiX control library, was performed at ~25 million reads/sample in single-end mode with 75 nt read length on the NextSeq 500 platform (Illumina) or 100 nt read lenght on the NextSeq 2000 platform (Illumina).