title: GSM9343593: LAO1KO mouse colon, DSS, replicate A; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from colon tissue using the ISOGEN II Reagent (Nippon Gene). The quality of the isolated total RNA was confirmed and the grade of RNA in all samples was unified. RNA-seq libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions. Sequencing was performed by Macrogen Japan on Illumina NovaSeqX platform with paired-end 150 bp reads.