title: GSM8504498: Glucose-Pyruvate cells, mRNA; Homo sapiens; OTHER
library_construction_protocol: Single Cell Multiome ATAC + Gene Expression was performed with 10X genomics system using Chromium Next GEM Single Cell Multiome Reagent Kit A (catalog no. 1000282) and ATAC Kit A (catalog no. 1000280). We followed Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits User Guide with the difference that single cell suspension was prepared almost as described in Mimitou et al., 28 (DOGMAseq LLL protocol with NP- 40 buffer). However, to further increase mitochondria DNA coverage, we optimized the lysis step by increasing NP-40 concentration to 0.3% and the time of lysis to 3'. Final libraries were sequenced on Illumina NovaSeq X+ platform (R1 – 28 cycles, i7 – 8 cycles, R2 – 90 cycles).