title: Gene expression profiling of melanoma cells following intracardiac injection in NOD-SCID mice: a comprehensive in vivo model
library_construction_protocol: The following melanoma cell lines namely LCP, LCM, WM266, SKMel28 and A375 were pursued from the American Type Culture Collection, USA and cultured in specific standard conditions following the manufacturer's instructions. RNA extraction was performed using the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. RNA-seq sequencing libraries were constructed by the Ion AmpliSeq™ Library Kit 2.0 (Life Technologies) starting from about 100 ng of the total RNA. 