title: GSM9293958: Postnatal day 5 SOM-Cre;Ai47 mouse sample-2; Mus musculus; RNA-Seq
library_construction_protocol: Fresh brains were taken from P5 ChC-GFP, SST-GFP, and VIP-GFP pups, and 300- to 400-μm-thick slices were prepared using a tissue chopper. Cortical strips containing L2/3 cells were dissected from the mPFC and the ACC and dissociated into individual cells in Hibernate E medium containing Pronase E (1 mg/ml) and DNaseI (5 U/mL) at 37°C for 10 min. After washing with Hibernate E medium once, one hundred fifty GFP+ cells were picked up using a glass micropipette under a fluorescence dissecting stereomicroscope. Total RNA was extracted by RNAeasy kit After RNA extraction, the samples were then kept on ice and processed for library preparation using Illumina Seq according to the manufacturer's protocol.