title: GSM8542228: Echinococcus multilocularis infected liver, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from right middle lung lobe using Maxwell® RSC simplyRNA Tissue Kit (Promega, AS1340) in combination with a ball mill (Retsch) Sequencing libraries were be prepared at the Core Facility Genomics, Medical University of Vienna, using the NEBNext UltraExpress rRNA Depletion Kit (NEB #E3330) according to manufacturer's protocols. Libraries were QC-checked on a Bioanalyzer 2100 (Agilent) using a High Sensitivity DNA Kit for correct insert size and quantified using Qubit dsDNA HS Assay (Invitrogen). Pooled libraries were sequenced on a P3 flowcell on a NextSeq2000 instrument (Illumina) in 1x50bp single-end sequencing mode.