title: RNA-Seq of human hematopoietic stem and progenitor cells to study membrane repair following electroporation (EP) or filtroporation (FP) disruption based strategies for intracellular delivery
library_construction_protocol: Wild type and KO Jurkat cells (American Type Culture Collection, Inc., ATCC) were cultured in 1× Roswell Park Memorial Institute Medium (RPMI) 1640. Total RNA was extracted and purified from HSPCs after cell lysis (~400,000 cells per condition in triplicates) using a Qiagen RNeasy Mini Kit.  RNA libraries for RNA-seq were prepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads following the manufacturer's protocol which included reverse transcription of mRNA, ligation of adaptors, and PCR enrichment of libraries. Libary pool was 10nM.