title: GSM9073024: Mouse liver ChIP IgG control biol rep 1; Mus musculus; ChIP-Seq
library_construction_protocol: Mouse livers were perfused and tissue was digested into a single cell suspensions. ChIP-sequencing DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina for the library prep (NEB E7645). For CUT&RUN DNA preparation, low passage number cell lines were harvested, and genomic binding sites were isolated according to the manufacturer's protocol (Epicypher 14-1048). NGS libraries were prepared according to the manufacturer's protocol (Epicypher 14-1001). Samples were sequenced with 50 bp paired end flow cells using an Illumina NextSeq2000 system