title: GSM8543616: 4NQO + SP2509 Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions from mouse oral cavities and oral cavity lesions dissociated as described above were loaded on a 10X genomics chip B at concentrations varying between 805,000 and 1,930,000 cells/mL with viability over 90% for a targeted cell recovery of 6,000 cells per sample and processed using the 10X Genomics 3'v3  kit according to the manufacturer's protocol (10x Genomics, CA, USA). Following the 10X Genomics Chromium controller, the gel beads in emulsion (GEMs) were transferred to strip tubes and subsequently placed into a thermal cycler using the following parameters: 45 min at 53 °C, 5 min at 85 °C, and hold at 4 °C. The samples were then stored overnight at -20 °C. The next day, the samples were thawed at room temperature before adding the recovery agent to break the GEMs. Subsequently, the cDNA was purified and amplified, and gene expression libraries were generated and purified 10X Genomics 3'v3  kit according to the manufacturer's protocol (10x Genomics, CA, USA).