title: GSM9307814: Patient_4_Basal; Homo sapiens; RNA-Seq
library_construction_protocol: The granulocytes layer was carefully taken, and lysed for RNA isolation by AllPrep DNA/RNA/protein mini kit (Qiagen, Hilden, Germany), following the manufacturer's protocol. 10 ng of total RNA from each sample was used as input for first-strand cDNA synthesis and subsequent cDNA amplification. Illumina sequencing libraries were prepared using the SMART-Seq mRNA LP protocol from Takara Bio (Takara Bio Inc, San Jose, USA), following the manufacturer's instructions.