title: GSM9232266: PT2, PB, GEM; Homo sapiens; OTHER
library_construction_protocol: Single-cell suspensions were obtained from tumor, non-tumor, and blood samples. Tumor tissues were digested enzymatically and filtered; PBMCs were isolated by Ficoll density gradient centrifugation. Cells were washed, counted, and viability was confirmed prior to library preparation. Single-cell 5' RNA-seq libraries were prepared using the Chromium Single Cell 5' Library and Gel Bead Kit (10X Genomics) following the manufacturer's protocol, and sequenced on an Illumina NovaSeq 6000.