title: Basal transcriptome of Arabidopsis amino acid transporter mutants
library_construction_protocol: Samples were harvested, washed once in sterile MilliQ water, and snap-frozen in liquid nitrogen Seeds were surface-sterilised and stratified for three days in the dark at 4C, then germinated on full-strength MS media (1% sucrose, 1% agar, pH5.8) for eight days at 22C under 24h light Seedlings were transferred from solid media to liquid half-strength MS Basal (pH5.7) media and incubated at 22Â°C for 48h under Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit Frozen samples are milled in extraction buffer and total RNA extracted using the QIAGEN Plant RNeasy kit