title: GSM8991392: 3A3A_NTC_30d; Homo sapiens; Bisulfite-Seq
library_construction_protocol: To enrich the EGFP+ cells, FACS sorting was performed after 72 h and the sorted cells were subjected directly for DNA, RNA and protein extraction using AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Venlo, Netherlands). we employed QIAseq Targeted Methyl Panel technology. The primer panels to enrich these regions were designed by QIAGEN (Hilden, Germany) both for the BACH2 (258 CpGs, 33 loci, 22,768 bp) and CXCR4 (212 CpGs, 13 loci, 10,871 bp) gRNAs (Supple. Table 2). Briefly, 110 ng DNA was bisulfite converted using EpiTect Fast Bisulfite Conversion Kit according to the manufacturer's instructions. Converted DNA fragments were attached to unique molecular identifier (UMI) and libraries were created using the QIAseq Targeted Methyl Panel protocol and were amplified for 18 cycles.