title: GSM9459793: whole colonic tissue, healthy control, T5224-treated, rep 3; Mus musculus; OTHER
library_construction_protocol: Genomic DNA was extracted using the Qiagen DNeasy Blood & Tissue Kit from OCT sections. For each sample, 200 ng of DNA was resuspended in water to 48 uL, mixed with control DNA from the NEBNext Enzymatic Methyl-seq V2 Kit total and sonicated on a Covaris S220 to 200 and 600 bp. Sonicated DNA was then processed per EM-seq V2 protocol