title: GSM8543462: WT_Smt3_IP_1; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Formaldehyde was add to mid-log phase cells at final concentration of 1% for 20 min. After quenching and cell lysis, sheared chromatin via sonication was incubated with anti-Flag M2 agarose beads and Protein A sepharose for mock IP control. Eluted chromatin fragments were treated with protease then DNA was extracted using a standard phenol/chloroform extraction method. ChIP-seq libraries were constructed using TruSeq DNA Sample prep kits and sequenced on an Illumina Novaseq 6000.