title: GSM8484033: 293FT cells transfected with BLU-VIPR containing NTC gRNA (replicate 2) (Dark); Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from cells exposed to light, and from cells kept in the dark, using the Monarch Genomic DNA Purification Kit following the manufacturer's protocol (New England Biolabs). A pooled sequencing librarry was generated by equimolar pooling of indexed genomic PCR products of endogenous NEAT1. The PCR products were isolated using QIAquick Gel Extraction Kit (Qiagen) and quality control was performed with the Agilent High Sensitivity D1000 ScreenTape System (Agilent Technologies).