title: GSM9050950: CUT&Tag for exogenous (V5-tagged) Mecp2 in murine neurons, biol rep 2; Mus musculus; OTHER
library_construction_protocol: Nuclei from 100,000 cells were isolated and immobilized on Concanavalin A-coated beads. They were then incubated overnight at 4°C with primary antibodies (anti-MeCP2, anti-V5, or anti-Smarcb1). Following incubation with a secondary antibody, pA-Tn5 transposase was applied to fragment and tag antibody-bound DNA sites. Following tagmentation, amplicon libraries were generated using the Nextera® DNA Library Prep Kit (Illumina). Barcoded libraries were pooled and sequenced on the NovaSeq 6000 platform.