title: GSM8890976: MT829_CUT_RUN_SALL4_SV20_WT_D5_rep1; Homo sapiens; OTHER
library_construction_protocol: RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, T2010). RNA libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760). 1 μg of total RNA input was used for each library. Libraries were sequenced on Illumina NextSeq 2000, producing 60bp paired-end reads. Barcoded CUT&RUN libraries were made with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) using NEBNext Multiplex Oligos Dual Index Primers for Illumina (NEB, E7600S), and sequenced on Illumina NextSeq2000, producing 50 bp paired-end reads Barcoded CHIP-seq libraries were made with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) using NEBNext Multiplex Oligos Dual Index Primers for Illumina (NEB, E7600S), and sequenced on Illumina NextSeq2000, producing 50 bp paired-end reads ATAC-seq was performed using 50,000 cells per sample. Libraries were prepared using the ATAC-seq Kit (Active Motif, 53150) and sequenced on either Illumina NextSeq2000, producing 60 bp paired-end reads or 150 bp paired-end reads.