title: GSM9372698: Cells isolated from KrasG12D;Ptf1a-CreERTM mouse #2; 4-Hydroxytamoxifen treatment for 1 day; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolation from cells using Qiagen RNeasy Plus Mini Kit (Cat. 74134) using manufacturer's instructions. A minimum of 23 ng/uL of RNA was submitted to GENEWIZ from Azenta Life Sciences. ERCC RNA Spike-In Mix (Cat: #4456740) from ThermoFisher Scientific, was added to normalized total RNA prior to library preparation per manufacturer's protocol. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA).