title: GSM9102530: vehicle, KO, 8h, Rep2; Mus musculus; RNA-Seq
library_construction_protocol: At the end of the treatment, the medium was removed, and cells were washed with 1X PBS and lysed with Trizol. RNA was extracted using the ReliaPrep™ RNA Cell Miniprep System (Promega – Z6012) according to the manufacturer's instructions. RNA-seq libraries were prepared using SMART-Seq® mRNA LP kit (Takara Bio) following the manufacturer's protocol.