title: GSM8889738: SF-8628-K27WT_rep4; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Qiagen RNeasy Mini Kit (Qiagen, Cat# 74104) following the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer. Only samples with RIN > 8.0 were used for downstream processing. RNA-seq libraries were prepared using standard Illumina protocols. Poly(A)+ RNA was isolated from total RNA, fragmented, and reverse transcribed to generate cDNA. Libraries were end-repaired, A-tailed, adapter-ligated, and PCR-amplified. Sequencing was performed on an Illumina platform with 150 bp paired-end reads.