title: GSM8504208: PC3_WT_2; Homo sapiens; OTHER
library_construction_protocol: Total RNA was isolated using Trizol reagent (Invitrogen), according to manufacturer's instructions. Rapid gel extraction was used, followed by overnight precipitation at −20 °C for RNA extraction from the polyacrylamide gels. Ribosomal RNA depletion was not performed. Prepared RPF libraries were analyzed on a 2100 Bioanalyzer using a 7500 chip (Agilent, Santa Clara, CA) and quantified by qPCR (Cat.no. 07960140001, Roche, Switzerland) and subsequently diluted to a 10 nM concentration. Next, the libraries were pooled and loaded with 1.5 pM on a NextSeq550Dx system (RUO mode) and sequenced with 75-base single-read using a 75 Cycles High Output Kit v2.5 (Illumina Inc., San Diego).