title: GSM8990031: Lc MV RNA 24h Z2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from the Caco-2 cells using the miRNeasy Micro Kit (Qiagen) according to the manufacturer's instructions. DNA libraries were prepared with the high-throughput MGISP-960 sample preparation system using the MGIEasy rRNA Depletion Kit (MGI Technologies) and MGIEasy RNA Library Prep Set (MGI Technologies) according to the manufacturer's recommendations. After PCR, different barcoded samples were pooled in equal amount and circularized to generate single stranded DNA libraries (ssDNA). DNA nanoball (DNB) were generated by rolling circle amplification. Paired-end sequencing (PE100) was performed using DNBSEQ-G400RS High-throughput Sequencing Reagent Set (MGI Technologies) on the DNBSEQ-G400 instrument.