title: GSM9131362: CTL, Donor AR012; Homo sapiens; RNA-Seq
library_construction_protocol: Skin biopsies were incubated overnight in 0.4% dispase (Life Technologies) in Hank's Balanced Saline Solution (Gibco) at 4°C. Epidermis and dermis were separated. Epidermis was digested in 0.25% Trypsin-EDTA (Gibco) with 10U/mL DNase I (Thermo Scientific) for 1 hour at 37°C, quenched with FBS (Atlanta Biologicals), and strained through a 40μM mesh. Dermis was minced, digested in 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma) in plain medium for 1.5 hours at 37°C, and strained through a 40μM mesh. Epidermal and dermal cells were combined in 1:1 ratio. The 10x Chromium system with chemistry v3 was used by the University of Michigan Advanced Genomics Core to generate libraries, which were subsequently sequenced on the Illumina NovaSeq 6000 platform, generating 150-base pair paired-end reads.