title: GSM8680433: Mouse 1, TTR>NICD-CAG>tdT HDTVi 3-weeks; Mus musculus; RNA-Seq
library_construction_protocol: Three weeks after HDTVi, the abdominal  vena cava was cannulated with the thoracic  vena cava clamped. The portal vein was snipped and 20 mL HBSS -/- buffer was perfused. Then, HBSS +/+ with 4 mg/mL Liberase was perfused until the liver was digested to acheive a single-cell suspension. Cells were washed with HBSS -/- and incubated with EpCAM fluorophore-conjugated antibody and DAPI. Cells were sorted on FACSymphony S6 into DMEM supplemented with 10% FBS. Manufacturers instructions were followed to partition cells into Gel Beads-in-emulsions (GEMs) together with unique 10X barcodes using the Chromium Controller. cDNA libraries were produced from GEMs to generate a barcoded single cell library for each mouse. Sequencing data were obtained using the Illumina NovaSeq 6000 platform.