title: GSM8505516: sham 1 ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were treated with 1% formaldehyde for 10 minutes at room temperature to crosslink proteins to DNA. Sonicate up to 500 μl of lysate with several pulses to disrupt the nuclear membrane. The library was constructed by Novogene Corporation (Beijing, China). Subsequently, pair-end sequencing of sample was performed on Illumina platform (Illumina, CA, USA). Library quality was assessed on the Agilent Bioanalyzer 2100 system.