title: GSM9375250: Th1 cells, Control cohort, Biol rep 7; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy Micro Kit (74004, Qiagen, Canada), as per manufacturer's guidelines. RNA purity was assessed using the Nanodrop Spectrophotometer (ThermoFisher, Canada) prior to storage at -80C. Total RNA purity was assessed prior to library preparation. RNA samples were processed at the McGill Genome Centre (Quebec, Canada) using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) with poly (A) mRNA enrichment. Libraries were generated according to standard NEBNext protocols including mRNA capture, fragmentation, first- and second-strand cDNA synthesis, end repair, adaptor ligation, and PCR amplification. Libraries underwent Ampure XP bead clean-up and quality assessment (LibQC), including removal of primer dimer fragments, before pooling and sequencing on an Illumina NovaSeq X Plus platform using paired-end reads.