title: GSM9293764: OCI-Ly7_HDAC3i_AZA_rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Lymphoma cells were pelleted, RNA was isolated using Trizol reagent and the Direct-zol RNA MicroPrep kit (Zymo Research) according to the manufacturers instructions mRNA enrichment was performed on total RNA using oligo(dT)-attached magnetic beads. The enriched mRNA with poly(A) tails was fragmented using a fragmentation buffer, followed by reverse transcription using random N6 primers to synthesize DNA double strands. The synthesized doublestranded DNA was then end-repaired and 5'-phosphorylated, with a protruding 'A' at the 3' end forming a blunt end, followed by ligation of a bubble-shaped adapter with a protruding 'T' at the 3' end. The ligation products were PCR amplified using specific primers. The PCR products were denatured to single strands, and then single-stranded circular DNA libraries were generated using a bridged primer. The constructed libraries were quality-checked and sequenced after passing the quality control.