title: E2F4-p53 Re-ChIP seq in HCT116 wild-type cells
library_construction_protocol: Cells were harvested from 6 well-plate, followed by trypsinization, crosslinking and fixaton. Cells were grown under asynchronous condition in McCoy media Cells were lysed using a nuclear lysis buffer. Followed by sonication of genomic DNA. 1ng of DNA used for library preparation. The Re-ChIP DNA libraries were constructed using the Kapa Hyperprep Kit with sample purification beads according to the manufacturerâ€™s instructions.