title: Neutrophil ifCAF conditioned media 18 samples bulk RNA-seq
library_construction_protocol: Co-cultures of  pancreatic stellate cells and tumour organoids were dissociated to single with dispase and trypsin. Cells were stained with DAPI for viability and with MHCII antibody. PSC were isolated based on mKate2 fluorescence and MHCII expression.  500 cells were sorted directly into lysis buffer without RNA extraction Cell lysates were reverse transcribed directly in the lysis buffer as per the Smart-Seq2 protocol. Libraries were generated using nextera technique as per Smart-Seq2 protocol.