title: RNA-seq of human melanoma cell line UACC-62 parental and TRIB2 knock-out clones
library_construction_protocol: Cells pellets were collected using Trypsin/EDTA centrifuged and pellet was immediately foren RNA was extracted using the Macherey-Nagel NucleoSpin RNA kit according to the manufacturer´s protocol. Total RNA (50 ng) was used to generate barcoded RNA-seq libraries using the NEBNext Ultra II Directional RNA Library preparation kit (New England Biolabs) according to manufacturer's instructions. First, poly A+ RNA was purified using poly-T oligo- attached magnetic beads followed by fragmentation and first and second cDNA strand synthesis. Next, cDNA ends were repaired and adenylated. The NEBNext adaptor was then ligated followed by second strand removal, uracile excision from the adaptor and PCR amplification.