title: GSM9051161: U138, siControl, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Qiazol (Qiagen, IT), purified from DNA contamination through a DNase I (Qiagen, IT) digestion step and further enriched by Qiagen RNeasy columns specific for gene expression profiling (Qiagen, IT). Quantity and integrity of the extracted RNA were assessed using a NanoDrop Spectrophotometer (NanoDrop Technologies, DE) and a TapeStation System (Agilent), respectively. RNA libraries for sequencing were generated in triplicate using the same amount of RNA for each sample according to the Illumina Stranded Total RNA Prep kit, with an initial ribosomal depletion step using Ribo-Zero Plus (Illumina, CA). The libraries were quantified by qPCR and sequenced in paired-end mode (2x100 bp) with NovaSeq 6000 System (Illumina, CA). For each sample generated by the Illumina platform, a pre-process step for quality control was performed to assess sequence data quality and to discard low-quality reads