title: RNA-Seq of An Integrated Signaling Network Controls P-Bodies Dynamics and mRNA Translation in Human Glioblastoma
library_construction_protocol: cells were collected using standard protocols  standard protocol standard protocols for cell lines cells where treated with compound for certain number of hours.  standard protocol Total RNA extraction was performed using the TRI reagent (Merck T9424) following the manufacturer's instruction Total RNA concentrations were determined by using NanoDrop spectrophotometer (Thermo Fisher) and their quality were assessed with the TapeStation 4150 (Agilent Technologies). Briefly, 100 ng of RNA of each sample were subjected to mRNAs capture by using polyT-coated beads followed by the indexed library preparation with the SureSelect XT HS2 mRNA Library Preparation System (Agilent Technologies), according to the manufacturerâ€™s instructions