title: GSM8504271: 501TS14; Amaranthus powellii; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Direct-zol RNA Miniprep kit by Zymo Research. Libraries were generated using 250 ng of total RNA. Enrichment of mRNA and library preparation were performed using Illumina Stranded mRNA Prep kit (Illumina) following manufacturer's instructions. A single sample of RNA extracted from susceptible tissue was used for PacBio sequencing. cDNA libraries were prepared using Pacific Biosciences Preparing Iso-Seq libraries using SMRTbell prep kit 3.0 protocol.