title: GSM8484869: Small intestine, Muscle, rep3; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspensions of GFP+ muscularis cells were stained with trypan blue to assess viability and cell concentration. Approximately 4,000 cells per sample were then loaded onto the 10X chromium controller. Libraries were prepped according to the manufacturer's protocols (Chromium Next GEM Single Cell 3' Reagent Kit v3.1 and dual index). Samples were pooled 1:1 prior to sequencing using the Illumina NextSeq 500 150 cycle kit.