title: C.elegans iron homoeostasis
library_construction_protocol: The worms were cultivated on 8P agar plates coated with NA22 E. coli at 20 °C, 750 worms were isolated after treatment for RNA isolation.  RNA was isolated from 750 worms in 1 mL TRI reagent® (Sigma-Aldrich). Isolated RNA was purified with the RNA clean and concentrator™-5 Kit (Zymo Research) according to the manufacturers instructions.  Library preparation was performed with 300 ng RNA using the NEBNext® Ultra™ II directional RNA library prep kit for Ilumina® with 14 PCR cycles according to manufacturers instructions.