title: GSM8855569: SETD2CI_input; Mus musculus; ChIP-Seq
library_construction_protocol: ESCs were fixed with 1% formaldehyde (Thermo Fisher) and sheared with E220 focused ultrasonicator (Covaris), respectively. Sheared chromatins were mixed with lysate from HEK293T cells processed identically as spike-in for normalization. Mixed chromatin were incubated at 4℃ overnight with antibodies. Protein A/G beads (Santa Cruz Biotechnology) were then incubated with the chromatin and antibody mixture for 2 hours at 4℃. Beads were washed, reverse-crosslinked, and DNA were purified with Qiaquick PCR purification kit (Qiagen). KAPA HyperPrep Kit (Roche) was used to prepare ChIP-Rx libraries.