title: GSM9340528: Rat kidney cortex, DEFND, 56 weeks, rat P16, ATAC; Rattus norvegicus; ATAC-seq
library_construction_protocol: Rat kidneys were dissected immediately following euthanasia and flash-frozen in liquid nitrogen. For each preparation, approximately ~35 mg of frozen tissue was processed. Tissues were mechanically dissociated using a gentleMACS™ Tissue Dissociator and nuclei were released by lysis under standard nuclei isolation conditions. Isolated nuclei were either processed using the standard 10x Genomics Chromium Multiome workflow (RNA + ATAC) or subjected to lithium diiodosalicylate pretreatment following the DEFND-seq protocol (Olsen et al. Nature Methods, 2025) to achieve nucleosome depletion. Final nuclei suspensions were loaded onto the 10x Genomics Chromium platform for droplet-based single-cell library preparation according to the manufacturer's protocol (Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide-Rev F). Libraries were generated following standard 10x Multiome (scRNA + scATAC) chemistry and sequenced on Illumina platforms.