title: GSM8889978: HA::alg-1, rde-4(syb6665), nrfl-1, RNAi, input, rep3; Caenorhabditis elegans; ncRNA-Seq
library_construction_protocol: Worms were washed from plates and rinsed with M9 3 times and flash frozed in liquid nitrogen. Total RNA from cell lysates or Argonaute co-immunoprecipitates was isolated using trizol/chloroform and isopropanol precipitation. Small RNAs (~16-30-nt) were purified from total RNA by size selection using 17% polyacrylamide gels and in some instances treated with NEB RNA 5' Pyrophosphohydrolase (RppH) to facilitate capture of 22G-RNAs. Libraries were then prepared according to the NEBNext Multiplex Small RNA kit protocol. PCR products (~134-148 bp) were size selected using 10% polyacrylamide gels.