title: GSM8567972: mmYS-pre, Young Sed, Pre-Ex, Male2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from powdered tissues were isolated using the RNeasy Plus Universal Mini Kit (Qiagen, Cat# 73404). 1.2 μg of total RNA was used to isolate poly(A)-containing RNA with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Cat# E7490S). 1 ng of poly(A)-mRNA isolates was used synthesize barcoded PCR-cDNA libraries (Oxford Nanopore Technologies®, Cat# SQK-PCB109).