title: GSM8505539: SLR14-5; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from tumors using the Qiagen RNeasy kit Library preparation was completed using the NEBNext® rRNA Depletion kit andprotocol(P/N: E7850X, NEB, Ipswich, MA) per manufacturer recommendations. The kit employs an RNaseH-based method to deplete both cytoplasmic and mitochondrial rRNA. After ribosomal depletion, the RNA samples underwent mRNA enrichment using oligo(dT) beads, followed by thermal fragmentation using divalent cations under elevated temperature. First-strand cDNA synthesis was performed using random hexamer primers, while second-strand cDNA synthesis was carried out using DNA Polymerase I and RNase H. End repair, A-tailing, and adapter ligation was performed to generate the final cDNA library. The library quality was assessed using a Bioanalyzer and quantified using a qPCR-based method with the KAPA Library Quantification Kit (P/N: KK4873) and the QuantStudio 12K instrument. NEBNext® rRNA Depletion