title: GSM8482365: Fresh tumor 3, B16F10 tumor bearing mice treated with M44 vaccine; Mus musculus; RNA-Seq
library_construction_protocol: Fresh tumor tissue was frozen in liquid nitrogen after dissection and then stored at -80 ℃. RNA was extracted from the tumors using the RNeasy Plus Mini Kit (Cat#74134, Qiagen, DE). RNA libraries for RNA-seq were prepared using Illumina TruseqTM RNA sample prep Kit following manufacturer's protocols.