title: GSM8568069: pbs-fat body-6h_3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was extracted using Trizol. 1 µg of total RNA was used for library construction. Eukaryotic mRNA was enriched using magnetic beads with Oligo(dT). Subsequently, fragmentation buffer was added to break the mRNA into short fragments. Using the mRNA as a template, first-strand cDNA was synthesized with random hexamer primers. Then, a buffer, dNTPs, DNA polymerase I, and RNase H were added to synthesize the second-strand cDNA, which was purified using AMPure XP beads. The purified double-stranded cDNA underwent end repair, A-tailing, and adapter ligation, followed by size selection using AMPure XP beads. Finally, PCR amplification was performed, and the PCR products were purified with AMPure XP beads to obtain the final library.