title: GSM8679789: Baseline_LNenzaR_bioRep2; Homo sapiens; OTHER
library_construction_protocol: From the collected cells, genomic DNA was isolated by PureLink Genomic DNA mini kit (Invitrogen, K1820) according to the manufacturer's instructions. Integrated sgRNA sequences were amplified by PCR reaction. For this, 4 μg genomic DNA was amplified for 25 cycles by using Kapa HiFi HotStart ReadyMix (Roche, KK2602) mixed with 10 μM external forward and reverse primers. To introduce sequencing adapters, external PCR products were pooled and amplified for 15 cycles using internal primers. PCR product was gel-isolated. After quality check, PCR products were sequenced by Genewiz, USA. Each screen was repeated three times.