title: GSM8989545: RNA-seq of EVs from uterine fluid , ART patient, sample 31; Homo sapiens; RNA-Seq
library_construction_protocol: Uterine fluid samples were obtained by lavage of the endometrial cavity with 2.5 of physiologic solution using a balloon hysterosonosonography catheter to avoid vaginal contamination. Extracellular vesicles were isolated- after mucus disgregation by vortexing- using standard ultracentrifugation protocol.  RNA was harvested using  RNase micro KIT of QIAGEN with DNAse treatment step in coloumn. To perform transcriptome sequencing on UF-EVs, cDNA libraries were prepared using the SMART-Seq V4 Ultra Low Input RNA kit