title: Transcriptomic analysis of the interactions between CDIPTOSP and STAU1
library_construction_protocol: OC cell lines (SKOV3, A2780, OVCAR3, HO8910 and CaoV3) and a normal ovarian epithelial cell line (IOSE80) were obtained from the Cell Bank of Chinese Academy of Sciences (Shanghai, China), and cultured in a humidified incubator at 37°C with 5% CO2. RPMI-1640 medium (Gibco, USA) supplemented with 1% penicillin/streptomycin (PS) (NCM Biotech, China) and 10% fetal bovine serum (FSP500, ExCell Bio, China) was used to culture IOSE80, SKOV3, A2780,HO8910 and CaoV3 cells. OVCAR3 cells were cultured in RPMI-1640 medium containing 20% fetal bovine serum. SiRNAs (Gene Pharma, Shanghai, China) targeting CDIPTOSP,STAU1, and a negative control (si-NC) were transfected into SKOV3 and A2780 cells using Lipofectamine 2000 (Invitrogen, USA). Total RNA was extracted from cells transfected for 48 hours using TRIzol reagent (Vazyme, Nanjing, China). Subsequently, reverse transcription was performed using the HiScript III RT SuperMix for qPCR kit (Vazyme) in accordance with the manufacturer's instructions.  RNA-Seq on SKOV3 cells was performed by LC-Bio Technology (Hangzhou, China), involving library preparation with poly-A enrichment, cDNA synthesis, and sequencing on an Novaseq™ 6000 platform.