title: GSM9600608: oxLDL-2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cultured CAFs using TRIzol reagent. Subsequently, small RNA fragments (18-30 nt) were enriched and purified via denaturing polyacrylamide gel electrophoresis to isolate high-quality miRNAs for sequencing. Purified miRNAs were sequentially ligated to 3' and 5' adapters. The ligated products were reverse-transcribed into cDNA and amplified by PCR. The final cDNA library was purified and validated for quality prior to sequencing.