title: GSM8484460: T2DM, ScRNA_seq; Rattus norvegicus; RNA-Seq
library_construction_protocol: We isolated the thoracic aorta and its perivascular adipose tissue (PVAT) from rats for single-cell RNA sequencing (scRNA-seq) and molecular biology experiments. Specifically, rats were anesthetized with an intraperitoneal injection of sodium pentobarbital (60 mg/kg) and perfused transcardially with saline to remove blood. The thoracic cavity was exposed via a midline sternotomy. Carefully, the lungs, liver, kidneys, and spleen were removed to fully expose the heart and aorta. The heart and thoracic aorta were excised and placed in ice-cold PBS. Under the microscope, the heart was removed, and the thoracic aorta along with its surrounding PVAT were carefully separated. The scRNA-seq libraries were constructed using a Chromium Single Cell 3' Reagent Kit v2 (10× Genomics) according to the manufacturer's protocol. Library quantification and quality were assessed by a Qubit fluorometer (Invitrogen)