title: GSM9460209: CJ_XC_LR_018_T; Homo sapiens; OTHER
library_construction_protocol: Cell suspensions were then washed, counted, pooled, concentrated, and subjected to 5′ single-cell RNA sequencing using the Chromium Next GEM Single Cell 5′ Kit v2 and Chromium Next GEM Chip K Single Cell Kit (10x Genomics, Pleasanton, CA, USA), targeting a recovery of 10,000 cells using manufacturer's protocols. Sequencing libraries were prepared using the Library Construction Kit (10x Genomics, Pleasanton, CA, USA) and sequenced on an Illumina NextSeq 2000. Cells were stained using TotalSeq-C hashtag and CITE-Seq antibodies. Cell suspensions were then washed, counted, pooled, concentrated, and subjected to 5′ single-cell RNA sequencing using the Chromium Next GEM Single Cell 5′ Kit v2 and Chromium Next GEM Chip K Single Cell Kit, targeting a recovery of 10,000 cells. Sequencing libraries were prepared using the 10X Genomics Library Construction Kit and sequenced on an Illumina NextSeq 2000.