title: GSM9372516: Ribo-Seq, Human CD8+ T cells, chronic, donor 5; Homo sapiens; OTHER
library_construction_protocol: Briefly, cells were lysed and triturated through a 26 G needle. Lysates were digested with P1 nuclease. Ribosomes were pelleted by sucrose cushion. Ribosome protected fragments were extracted using the mirRICH method (Choi et al., 2018). Library generation was carried out using the OTTR cDNA library construction kit (Karnateq) following the manufacturer's protocol. 40 ng of RNA per sample was used as input for the OTTR reaction.