title: GSM8990425: Non-senescent BPH epithelial rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Invitrogen) following the manufacturer's instructions. RNA quality was assessed using NanoDrop and Bioanalyzer. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Poly(A)+ RNA was purified, fragmented, and reverse-transcribed. Libraries were adapter-ligated, PCR-amplified, and quality-checked.