title: GSM8483704: hESC_DE_wt_2, ATACseq; Homo sapiens; ATAC-seq
library_construction_protocol: hESC-DE cells were collected from cultures and 50,000 cells for each sample were lysed using an adjusted lysis buffer based on the Omni-ATAC protocol, followed by a transposition reaction with hyperactive Tn5 transposase. The DNA fragments were purified using a MinElute Reaction Cleanup Kit (Qiagen, Germantown, MD). Library construction was performed according to the manufacturer's recommendations of the Nextera DNA Prep Kit. Libraries were purified using AMPure XP beads to remove primer dimers. The library quality was evaluated using the TapeStation D1000 ScreenTape.