title: GSM9342064: Clrn1-/-Clrn2-/-_Rep2_Organ of Corti P21; Mus musculus; RNA-Seq
library_construction_protocol: RNA extraction and purification was performed using the NucleoSpin RNA, Mini kit for RNA purification (Macherey-Nagel, 740955.50) RNA libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina protocol according supplier recommendations. Briefly, the key stages of the protocol are successively: the purification of Poly-A containing mRNA molecules using poly-T oligo attached magnetic beads from 100ng total RNA (with the Magnetic mRNA Isolation Kit from NEB), a fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces, double strand cDNA synthesis and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing.