title: GSM9252206: SCCE-P3; Homo sapiens; RNA-Seq
library_construction_protocol: 50 μm FFPE curls were dissociated with the Bioyou® Nuclei lsolation Kit for FFPE Tissue (52301-10, Shanghai Biotechnology Corporation). Approximately 600,000 nucleus were washed, counted, and resuspended, loading 16,000 nucleus per each of four GEM wells (targeting 10,000 recovered cells) on a single Chromium X chip. Sequencing libraries were generated following the User Guide (CG000477). Sequencing libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). Libraries were sequenced on an Illumina NovaSeq6000 (Illumina) using 2x150 chemistry. 50 μm FFPE curls were dissociated with the Bioyou® Nuclei lsolation Kit for FFPE Tissue (52301-10, Shanghai Biotechnology Corporation). Approximately 600,000 nucleus were washed, counted, and resuspended, loading 16,000 nucleus per each of four GEM wells (targeting 10,000 recovered cells) on a single Chromium X chip. Sequencing libraries were generated following the User Guide (CG000477). Sequencing libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). Libraries were sequenced on an Illumina NovaSeq6000 (Illumina) using 2x150 chemistry.