title: GSM9376144: Pig brain cervical spinal cord, Animal ID 14-9, male, UBE3A mat. deletion; Sus scrofa; RNA-Seq
library_construction_protocol: Tissues were flash frozen on liquid nitrogen and stored at -80*C until use. RNA was extracted from 20 mg of tissue using the Qiagen RNeasy kit (74104) following the manufacturer's protocol. RNA quantity was verified using the Qubit RNA BR assay (Thermo Fisher Scientific, Q10210) and quality was checked using an Agilent TapeStation.  RNA libraries were prepared from 1 µg of total RNA with RIN > 6.1 by TIGSS using the TruSeq mRNA stranded kit (Illumina, 20020595).  Paired end, 150 base pair sequencing on the NovaSeq 6000 at a depth of 10-20 million reads per sample was performed.