title: GSM9103315: rrp1Δ-expA-1; Schizosaccharomyces pombe; MNase-Seq
library_construction_protocol: MNase-seq was performed by adapting a published protocol (Lanterman et. al, 2009). Briefly, cells were fixed with formaldehyde, and converted to spheroplasts using Zymolyase. Chromatin was digested with MNase, and DNA was extracted using phenol-chloroform and ethanol precipitation. Mononucleosomal DNA was size-selected via agarose gel extraction. Library preparation was performed using the NEBNext Ultra II kit (NEB Cat. No. E7645L) with custom barcoded adapters. Libraries were quality-checked using an Agilent Fragment Analyzer and quantified with a Kapa Library Quantification Kit (Roche Cat. No. KK4903). Sequencing was conducted on an Illumina sequencer.