title: GSM8542574: murine plexiform neurofibroma, snRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: PNF bearing sciatic nerve tissues pooled from five, 11-month-old Nf1flox/flox;PostnCre+ mice were harvested, flash frozen at -80°C and shipped on dry ice to Novogene for single nucleus capture, library preparation and sequencing.  Single nuclei suspensions were loaded onto Chromium Chips (10x Genomics) for 3' library preparation according to the manufacturer's recommendations. Library quality control was performed by Qubit 2.0 for preliminary determination of the library concentration, Agilent 2100 to determine the insert size, and Q-PCR to precisely quantify the library effective concentration.