title: GSM9202161: 08_Bacillus_IgG_male_Rep12; Bacillus grandii; OTHER
library_construction_protocol: heads of D.melanogaster and B.grandii were dissected and homogenized  in 100 µl wash buffer (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1x protease inhibitor (Sigma P8340) in nuclease-free H2O) using a handheld pestle. CUT&Tag libraries were amplified using NEBNext Ultra II Q5 Master Mix with standard protocols. Libraries were cleaned up using Ampure XP beads and were pooled together for paired-end sequencing.