title: GSM8504452: UM-Chor1, T-DARPin_B1, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Cell pellets were resuspended with 350 μL RLT buffer on ice, lysed with a 26-gauge needle, and processed with the RNAeasy Mini Kit (Qiagen), including a DNAse I digestion step. Eluted RNA was immediately frozen at −80°C with 1 µL RNaseOUT (Invitrogen) after quantification with a NanoDrop One system (Thermo Scientific). RNA purity was confirmed (RIN > 8) on a TapeStation (Agilent). Libraries were prepared using Illumina TruSeq Stranded Kit according to manufacturer's recommendations.