title: GSM8991176: PBS_CXCL10_1; Homo sapiens/Mus musculus xenograft; RNA-Seq
library_construction_protocol: Tumors were dissociated using the Tumor Dissociation Kit, mouse (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's protocol. The single-cell suspension was fixed using the Evercode Cell Fixation Kit (Parse Biosciences, Seattle, WA) and libraries were constructed using the Parse Biosciences platform according to the manufacturer's protocol. The Parse Biosciences Evercode WT Mini protocol, a simplified version of their scRNA-seq workflow, uses a split-pool combinatorial barcoding approach to profile single-cell transcriptomes without microfluidics. Instead of compartmentalizing cells into droplets, the protocol treats each cell as its own reaction compartment, using a series of barcoding rounds to label transcripts with unique sequences. This allows for scalable analysis of many cells and samples with standard lab equipment. Libraries were sequenced on Illumina NextSeq 2000 system (RRID:SCR_023614, Illumina, Inc., San Diego, CA).