title: GSM9101268: Frontal_RE_P018; Homo sapiens; RNA-Seq
library_construction_protocol: Brain tissue samples from epileptogenic regions were collected during resective epilepsy surgery, immediately snap-frozen on dry ice, and stored at -80°C until processing. Nuclei were isolated by mechanical and enzymatic dissociation of frozen tissue following 10X Genomics guidelines for single-nucleus RNA-seq. Isolated nuclei were filtered and counted prior to library preparation. Single-nucleus RNA libraries were constructed using the 10X Genomics Chromium Single Cell 3' v3.1 kit, following the manufacturer's Nuc-Seq protocol. Approximately 7,500 nuclei per sample were encapsulated and barcoded using the Chromium controller. Libraries were prepared with a target depth of ~150,000 reads per nucleus, following 10X Genomics recommendations.