title: Impact of Aging on CD146+ Mesenchymal Stromal Cells-Mediated Regulation of Bone Marrow CD34⁺ Hematopoietic Stem/Progenitor Cell Mobilization
library_construction_protocol: BM cells were isolated from femoral heads, otherwise discarded as surgical waste, obtained upon written informed consent from subjects undergoing hip replacement surgery due to coxarthrosis.  CD146⁺ BMSCs from each subject were plated at a density of 1.5 × 10⁵ cells per well in 6-well plates. After 3 days of incubation the total RNA was isolated using the miRNeasy Kit (QIAGEN, Hilden, Germany; cat # 217084), including an on-column DNase digestion step. RNA was subsequently precipitated with ethanol to further improve purity. Sequencing libraries were generated from 400 ng of total RNA as input with the TruSeq Stranded Total RNA kit (Illumina), according to the manufacturer's instruction.