title: Methylation mimic mutations of progesterone receptor AF1 impair gene-specific regulation through stabilized chromatin interactions
library_construction_protocol: Following hormone treatment, the culture media in 60-mm dishes were removed thoroughly. 1ml of TRIzol was added to each plate for cell lysis. The cultured cells were lysed in cold TRIzol reagent, and total RNA was isolated according to the manufacturer's protocol (Life Technologies, MD, USA). RNA was precipitated using isopropanol and pelleted at 12000xg, 4°C for 15 minutes. The pelleted RNA was washed twice with 75% ethanol prepared in DEPC-treated water, briefly air-dried and resuspended in DEPC-treated water.  . DNA free RNA samples were submitted to Genome Institute of Singapore (Agency for Science, Technology and Research) for library preparation and paired-end sequencing using Illumina HiSeq4000 platform. 