title: GSM9293054: 786-O cells, shRNA Control, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA sequencing from control and METTL3-knockdown 786-O cells, total RNAs isolated from 786-O cells stably expressing either control shRNA or METTL3-targeted shRNA were subjected to RNA sequencing using the DNBSEQ platform from BGI Genomics (San Jose, CA, USA). Briefly, high-quality (RIN>8) total RNAs isolated from cultured cells by the RNA miniprep kit (Zymo Research) were rRNA-depleted, fragmented (~200 bp), and converted to ds-cDNA. After adapter ligation, size-selected (~200-300 bp) sequencing libraries were prepared, which were then used to produce DNA nanoballs (DNBs), following BGI's DNBSEQ protocol. Sequencing was performed in the DNBSEQ-G400 platform, generating >50 million reads/samples with paired-end 150 bp reads.