title: GSM8821723: EV control (replicate 2), WAPL ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde for 10 min and quenched with 0.4M glycine for 5 min ChIP assays were performed according to the ENCODE protocol with modifications. For anti-WAPL ChIP (Proteintech, 16370-1-AP), 20 million cells per ChIP were used, with 5 μg of antibody for each ChIP. ChIP DNA was purified, and 10 μl of the ChIP DNA was used as input to construct the ChIP library using the ThruPLEX DNA-Seq Kit (Takara, R400676). The input DNA from the same biological replicate was used to construct the corresponding ChIP input library.