title: GSM8543618: Root, control (R), 15DAT, replicate 2; Oryza sativa; RNA-Seq
library_construction_protocol: Rice plants were rinsed with distilled water and the distal portions, up to 1 cm from the apices, of rice roots were cut with a sterile blade and immediately frozen in liquid nitrogen. RNA from the roots of three biological replicates per treatment at 15 DAT was isolated with Qiagen RNeasy Plant Mini Kit and treated with Qiagen Rnase-Free Dnase Set. Stranded mRNA libraries were prepared by Novogene with poly-T oligo-attached magnetic beads, followed by random hexamer priming and second strand cDNA synthesis with dUTP.