title: GSM8830376: IP; Homo sapiens; OTHER
library_construction_protocol: Hyperactive pG-MNase CUT&RUN Assay Kit (Vazyme, China) was used to perform the experiment according to the manufacturer's instructions. Living hASCs were collected and bound using Concanavalin A-coated magnetic beads pro. Cell membranes were permeabilized using Digitonin. Through the mediation of PHC2/IgG antibody and Protein G, the MNase nuclease fused to Protein G targeted and cleaved the DNA sequence near the target protein. Finally, DNA fragmentation was terminated and released, and DNA extraction and purification were conducted. Immunoprecipitated DNA was used to construct sequencing libraries following the protocol provided by the I NEXTFLEX® ChIP-Seq Library Prep Kit for Illumina® Sequencing（NOVA-5143-02, Bioo Scientific） and sequenced on Illumina Novaseq 6000 with PE 150 method.