title: GSM9491684: SAS cell Negative Control; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent following the manufacturer's instructions. RNA quality and quantity were assessed using a NanoDrop spectrophotometer and Agilent Bioanalyzer. Total RNA was extracted and quality controlled using Nanodrop and Qubit. mRNA was enriched using Oligo(dT) magnetic beads. Sequencing libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit (or equivalent). The workflow included mRNA fragmentation, cDNA synthesis using random primers, end repair, A-tailing, adapter ligation, PCR amplification, and purification with AMPure XP beads. Library quality was assessed by Qubit and Agilent Bioanalyzer prior to sequencing.