title: GSM9426594: MC38-high-CD47; Mus musculus; OTHER
library_construction_protocol: Ccells were resuspended in PBS for flow cytometry selection (the top 30% with the highest signals and bottom 30% with the lowest signals cell populations, respectively). Cells were collected for genomic DNA extraction (Qiagen Kit). PCR reaction was performed to amplify gRNAs inserts via primers harboring Illumina TruSeq adapters with i5 and i7 barcodes. The resulting libraries were sequenced using an Illumina HiSeq 2500 system. The Mouse Toronto KnockOut (mTKO) CRISPR Library contains 94,528 gRNAs targeting 19,463 protein-coding genes (4 gRNAs/ gene) and 418 EGFP-, LacZ-, and luciferase-targeted control gRNAs.