title: GSM8484744: Unexposed animals, sample 1b; Mus musculus; RNA-Seq
library_construction_protocol: Portions of the ventral brainstem were dissected and tissue was manually dissociated with a dounce homogenizer. Nuclei were then isolated using differential centrifugation. Enrichment for cholinergic nuclei was achieved by collecting GFP+ nuclei via FACS. Individual nuclei were captured through the 10x Genomics Platform (3' Kit v. 3.1). Libraries were generated following manufacturer's directions (10x Single Cell 3' v3.1). Some samples were sequenced multiple times to achieve sufficient coverage. Paired experimental and control samples were always multiplexed and sequenced together on a flow cell.