title: GSM8679806: GF tissue, 6M after graft, 4; Sus scrofa; RNA-Seq
library_construction_protocol: RNA was isolated using Trizol reagent (Invitrogen). RNA quality was assessed by Agilent 4200 TapeStation System (Agilent Technologies), and RNA quantification was performed using Qubit (Thermo Fisher). the construction of library was performed using QuantSeq 3' mRNA-Seq Library Prep Kit (Lexogen, Austria) according to the manufacturer's instructions.