title: GSM9558142: RNA-Seq for control hSCs - Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from SCs cultures by TriFast Il (Euroclone, Pero, Italy),according to the manufacturer's protocol, then quantified with Nano-Drop2000 (ThermoFisher Scientific, Monza, Italy). Each sample of 500 ng total RNA was processed to obtain a libraryof indexed fragments using an Illumina Stranded mRNA kit (San Diego, CA, USA).Equimolar quantities of indexed libraries for each sample were loaded for sequencing ontwo flow cells Illumina MidOutput 150 cycles and sequenced using IlluminaNextSeq550dx (RUO mode).