title: Plasmodesmal closure triggers stress responses
library_construction_protocol: Leaf material was collected from 5 week old plants and frozen in liquid nitrogen following dissection. Leaf discs were cut with a tissue corer from three plants and were pooled as a single biological replicate. Arabidopsis thaliana plants were grown on MS plates for 5 weeks at 10 h light and 14h dark at 22 Â°C. Leaves were painted with either a mock treatment (water + 0.001% DMSO) or estradiol (20uM + 0.001% DMSO). Leaf tissue was homogenised using a Genogrinder and each Eppendorf tube contained 2 steel ball bearings.  Total RNA was extracted from leaf tissue using the Qiagen Plant RNeasy mini kit according to the manufacturers instructions. Samples were given rigorous treatment with Turbo DNase was undertaken to remove genomic DNA contamination.  Library construction was outsourced to Novogene. Libraries were constructed for paired end reads, using oligo-dT enrichment.