title: GSM8891927: RNA-Seq, GaHV-3-SB-1-infected ESCDL-1 cell line; Gallus gallus; RNA-Seq
library_construction_protocol: RNA was extracted using TRI-reagent (Thermofisher) and 5ug were sent to sequencing (Novogene). 5µg of total RNA was sent to Novogene for the library preparation and sequencing. Library preparation include : ribosomal depletion, linear RNA degradation, RNA fragmentation, reverse transcription, second strand synthesis (using dUTPs), end-repair, A-tailing, adapter ligation, enzyme digestion to remove dUTPs-containing second strand cDNAs, size selection, PCR amplification