title: GSM8890456: Bone marrow-derived MSC, aplastic anemia, male, 15 years age, cell passage number = P6; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was performed by using High Pure RNA Isolation Kit (Roche) according to the manufacturer's protocol and RNA was quantified in NanoDrop® (Thermo Fisher Scientific). Quality control (QC) of RNA samples was then performed with Fragment Analyzer™ (Agilent Technologies) and analyzed with ProSize data analysis software 4.0.2.7. Full-length complementary DNAs (cDNAs) were generated following the SMART-Seq2 protocol as described by Picelli, 2014. Quality control of cDNA was performed with Fragment Analyzer™ (Agilent Technologies) and ProSize data analysis software 4.0.2.7. All samples passed QC. Library preparation was performed following the Nextera library preparation protocol (Nextera® XT DNA Library Preparation kit, Illumina), as previously described by Baym, 2015. Libraries were confirmed by Fragment Analyzer™ (Agilent Technologies) and then sequenced in NextSeq2000 (Illumina). 100 bp single-end reads were obtained. For this experiment, a yield of about 25 million reads per sample was requested.