title: GSM9103651: U-2 OS Opto-IRE1, blue light + siXBP1 24 hours, rep 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolated with TRIzol and chloroform, followed by purification with a spin-column kit (RNA Clean & Concentrator-5, Zymo Research #R1015). polyA-RNA was reverse transcribed with poly-dT barcoded oligos and PCR amplified in the manner of SMARTer cDNA synthesis. This cDNA was multiplexed, cleaned, and prepared via the Oxford Nanopore Ligation Sequencing v14 kit.