title: scRNA seq of MG53high and MG53low TAMs
library_construction_protocol: We sorted live, CD45+ cells from single-cell suspensions of tumors from 3 mice using a FACS (BD Biosciences).  The single-cell suspension was subjected to quality control with the following criteria: cell viability must meet the requirements (>80% by AO/PI fluorescent staining or >75% by trypan blue exclusion), cell concentration between 700-1,200 cells/μL, cell diameter ranging from 5 to 30 μm, total cell count up to 100,000, clean suspension background with no significant debris or impurities. After quality control, the viable cells were washed, resuspended, and prepared as a single-cell suspension at an appropriate concentration. The cell suspension was then loaded according to the target cell recovery count. Following loading, the formation of GEMs (Gel Bead-In-Emulsions) was monitored. If GEMs formed successfully, they were transferred to a PCR tube for subsequent reverse transcription and library construction. Once the constructed library passed quality control, it was subjected to sequencing.