title: GSM9103589: freshwater sponge w/algae, light, S2_Emu5; Ephydatia muelleri; RNA-Seq
library_construction_protocol: Total RNA extracted from flash frozen cultured sponge tissue using Norgen Biotek Animal tissue kit Poly(A) RNA sequencing library was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. RNA integrity was checked with Agilent Technologies 2100 Bioanalyzer. Poly(A) tail-containing mRNAs were purified using oligo-(dT) magnetic beads with two rounds of purification.