title: GSM9252628: Intestinal epithelial cells from the ileum C57BL/6J mouse 7; Mus musculus; RNA-Seq
library_construction_protocol: Intestinal segments (duodenum and ileum; ~50mg) from Vipr1fl and Vipr1∆Villin mice were snap frozen and pulverised on dry ice. Tissues were resuspended in fixation buffer and fixed overnight according to 10x Genomics Demonstrated protocol for tissue fixation and dissociation (CG000553). Samples were quenched, dissociated on GentleMACS Octo dissociator, stained with AO/PI and counted on Luna Fx7 following the same manual. Cells were then hybridised overnight with 10x Genomics Mouse probes for Flex protocols, according to the 10x Genomics Flex protocol for Multiplexed samples (CG000527). The rest of the steps in the protocol included hybridisation, counting on Luna Fx7 and pooling the samples into one reaction which was loaded onto 10x Genomics chip. Libraries were also constructed according to the manufacturers protocol.