title: GSM9253009: Heart_Ex1_WT_1; Mus musculus; RNA-Seq
library_construction_protocol: Flash-frozen cardiac samples were homogenized in 1 ml of TRIzol (Thermo Fisher Scientific, 15596026) using Precellys Evolution. RNA was isolated using RNeasy Micro Kit (Qiagen, 74004) as per the provider's instructions. RNA-seq libraries (n=3 mice per group) were prepared using KAPA mRNA HyperPrep (Kapa Biosystems, kk8580) kit following manufacturer's specifications.Paired-end sequencing (50 cycles) was performed on a NextSeq 2000 Sequencer (Illumina)