title: GSM8715059: Control_KD_RIP_input; Homo sapiens; RIP-Seq
library_construction_protocol: RNA from RIP sample or whole-cell samplewas extracted with TRIzol reagent (Invitrogen) according to the manufacturer's protocol. The RNA from RIP sample or whole-cell sample were utilized to construct whole transcriptome library by the TruSeq Ribo Profile Library Prep Kit (Illumina, United States), following manufacturer's instructions. In brief, rRNA was removed by Illumina Ribo-Zero Gold kit and surplus RNA was purified for end repair and 50-adaptor ligation. Then, reverse transcription was performed with random primers containing 3' adaptor sequences. Finally, the cDNAs were purified and amplified with PCR reaction. The products with 300-500 length were purified and quantified. These libraries were utilized to 150 nt paired-end sequencing with an Illumina Nova seq 6000 system (Novogene, China). Each library was generated a depth of ~8G basepairs (for mRNA-seq sample) or ~6G basepairs (for RIP sample), and then adapters were removed with cutadapt software to obtain cleandata.