title: Historical RNA-seq of Jacquinia keyensis: Jacquinia-Herbarium
design: Leaf tissue was collected from an herbarium press tissue packet. Approximately 40 mg of tissue was ground to a fine powder in 1.5 ml tubes that were exposed to liquid nitrogen. RNA was extracted using the Sigma Spectrum Plant Total RNA kit (Spectrum Plant Total RNA Kit ; Sigma ; Cat no STRN50) with additional DNase digestion step (On-Column DNase I Digestion Set ; Sigma ; Cat no DNASE70) following protocol modifications explained in (https://doi.org/10.1093/aob/mcae002). RNA concentration and purity was assessed with a NanoDrop Lite Spectrophotometer (Thermo Scientific). Ribosomal RNAs were removed using the Fastselect kit (Qiagen), and the RNAseq libraries were prepared with the Kapa Hyper Stranded mRNA library kit (Roche). Paired-end 150 bp was carried out on a single lane of an Illumina NovaSeq X.