title: MMEJ reporter, ISce1 cut, Polqi treated
design: Genomic DNA was extracted using QuickExtract (Biosearch Technologies, #QE09050). One PCR reaction was done on each condition using Illumina MiSeq adapter sequences linked to primers that amplify a 78 bp sequence around the ISceI cut region of the reporter. Amplification was done in 50 L reactions with Q5 High-Fidelity 2X Master Mix (New England Biolabs, #M0492S), 1 g template, and 0.25 M of primers for 30 cycles. Products were purified using gel extraction of the bands in 0.6% agarose gel. Using manufacturer instructions, concentration was calculated using TapeStation 4150 (Agilent, #G2992AA). Libraries were pooled at 2.5 nM and sequenced at the University of Colorado Genomics Shared Resource (RRID:SCR_021984).