title: GSM8857474: ATACseq_MCF7_KO_NT_rep1; Homo sapiens; ATAC-seq
library_construction_protocol: Sample preparation was conducted according to omni-ATAC protocol previously described by Grandi et al.  Briefly, 50,000 cells per sample were used, and for each condition three replicates were analyzed. Cell lysis was performed using ATAC-seq Lysis Buffer (10 mM Tris–HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2, 0.1% NP40, 0.1% Tween-20 and 0.01% Digitonin). Following washes (10 mM Tris–HCl pH 7.5, 10 mM NaCl, 3 mM MgCl2 and 0.1% Tween-20) nuclei were incubated with 2.5ul of Nextera Tn5 enzyme (TDE1, Illumina) for 0.5h at 37°C. Transposition was terminated by adding 5 volumes of DNA Binding Buffer from the DNA Clean and Concentrator-5 Kit (Zymo Research, D4014) and tagmentated DNA was isolated using the same kit. Libraries were prepared using NEBNext Ultra II Q5 2× Master Mix (New England Biolabs, M0544S), followed by library quantification using NEBNext Library Quant Kit (New England Biolabs, E7630) and additional amplification. DNA concentration was measured with a Qubit fluorometer (Life Technologies) and library sizes were determined using TapeStation (Agilent Technologies).