title: Oxford Nanopore sequencing of Pseudomonas aeruginosa: 5hmC-modified PAO1 strain genomic DNA
design: Based on the genome assembly from BioProject PRJNA331 (RefSeq assembly: GCF_000006765.1, assembly name: ASM676v1). The cytosine in the library are fully substitute by 5hmC during PCR amplification. Library preparation was performed using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-LSK110) following the manufacturers protocol. The sequencing was carried out on a Nanopore MinION device. Reads are basecalled with reinforced 5hmC DeepSME basecaller at https://github.com/sparkcyf/DeepSME.