title: GSM8601836: RF054_S2_3; Homo sapiens; RNA-Seq
library_construction_protocol: NucleoSpin RNA Plus, Mini kit for RNA purification with DNA removal column MACHEREY-NAGEL Item number: 740984.50 NEBNext Ultra II Directional RNA Library Prep with ribodepletion We performed a NEBNext Ultra II Directional RNA Library Prep with ribodepletion starting from 51 ng of RNAs (see table below), according to NEB protocol NEBNext Ultra II Directional RNA Library Prep Kit, Version 4.0_4/21 (internal exp code PHX056). We quantified the resulting libraries by qubit DNA HS and performed profile analysis by TS4200. All libraries had sufficient yields (see table below), and all looked very good apart from RF053_S3_2 and RF054_M_3, which were still OK but with suboptimal characteristics (see comments in the table below).