title: AMO-1 cell line after 8h of Ceritinib, Carfilzomib treatment and their combination
design: AMO-1 cell line after 8h of Ceritinib, Carfilzomib treatment and their combination
library_construction_protocol: Cell were harvested form T75 flask and washed 2x with PBS and snap frozen in Liquid nitrogen Cells were grown in RPMI medium supplemented with 10% FBS and anitbiotics Cells were treated with Carfilzomib 5nM or Ceritinib 4uM or combination or without for 8h Total RNA was extracted by BGI from the submitted tissue samples using TRIzol reagent or a similar phenol-based method, following their standard RNA extraction workflow. RNA integrity and quantity were assessed using Bioanalyzer or similar QC platforms polyadenylated RNA was enriched using oligo(dT) magnetic beads. First- and second-strand cDNA synthesis was performed using reverse transcriptase and random hexamer primers. After end repair, A-tailing, and adaptor ligation, libraries were amplified by PCR. The final libraries were quality-checked using Qubit and Bioanalyzer before sequencing. Library construction followed BGIâ€™s standard mRNA-seq protocol