title: CUT&RUN of H3K4me1 in mESC
design: CUT&RUN of H3K4me1 in mESC
library_construction_protocol: 3 biological replicates of 250,000 cells/replicate were processed for each condition. Nuclei were pelleted and frozen after extraction in an isopropyl chamber, and then later thawed on ice to be processed for CUT&RUN in parallel.  CUT&RUN LoV-U for H3K4me was performed as described in (PMID: 36355069), with the following modifications. Antibodies used included anti-H3K4me (antibodies online, ABIN3023251) and anti-rabbit IgG (antibodies online, ABIN101961) at 1:100 dilutions. Library preparation was done using the KAPA Hyper Prep Kit for Illumina sequencing (Cat. #KK8504, KAPA Biosystems) following the manufacturerâ€™s guidelines with modifications, as described in (PMID: 36355069).