title: GSM8600829: atac.wt.treatment_brm014_2uM.dox; Homo sapiens; ATAC-seq
library_construction_protocol: ATAC-seq samples were generated as previously described (Orlando et al. 2020) with the following modifications: 50,000 cells per reaction, omni-ATAC was performed for cell lysis steps (Corces et al. 2017, Grandi et al. 2022) and Tn5 to volume ratio was 1:10. ATAC-seq libraries were generated by PCR amplification for 5 cycles followed by qPCR for determining remaining cycle numbers (~1/3 curve). Following DNA purification with Qiagen MinElute kit, final libraries were double-sided size selected (0.5X and 1.8X) with AMPure beads.