title: Gene expression and immune cell heterogeneity in inbred Amur tiger
design: Gene expression and immune cell heterogeneity in inbred Amur tiger
library_construction_protocol: All samples were collected with the informed consent of the owners, and the collection process was approved by the Laboratory Animal Welfare and Ethics Committee of Jilin Agricultural University. Firstly, the cell suspension was placed into Chromium microfluidic chips with 3' chemistry, and a 10x Chromium Controller (10X Genomics) was employed to barcode the chips. Afterward, the RNA from barcoded cells was reverse-transcribed, and sequencing libraries were constructed using the Chromium Single Cell 3' reagent kit (10X Genomics) according to the manufacturer's instructions. 