title: WGS of Bacillus sp. Fil: Christchurch soil isolate
design: Genomic DNA was extracted from a single colony of Bacillus sp. Fil grown on R2A agar using REDExtract-N-Amp Tissue PCR Kit, followed by clean-up with Zymo Clean & Concentrate. Paired-end 151-bp libraries were prepared using the tagmentation- and PCR-based Illumina DNA Prep kit with custom 10-bp unique dual indices, targeting a 280 bp insert size. Libraries were quality-checked on an Agilent TapeStation and sequenced on the Illumina NovaSeq X Plus platform.