title: RNA-Seq of Oreochromis niloticus, nirwana: adult female gonad
design: mRNA was enriched from the total RNA with poly-A enrichment module. mRNA sample was fragmented and hybridized with helper Adaptor Mix, followed by the adapter ligation. cDNA synthesis was performed using the adapter-ligated RNA, followed by PCR amplification to generate the final library sample. The quality and quantity of library samples were determined using Tape Station and Qubit Fluorometer, respectively. Sequencing was conducted 300 cycles (PE150) using Illumina NextSeq 2000