title: GSM9253257: corresponding adjacent tissues of patient 2; Homo sapiens; RNA-Seq
library_construction_protocol: cervical squamous cell carcinoma tissues and corresponding adjacent tissues were taken by pathologists from normal and tumor regions. Tissues were washed by PBS and put into a gentle Tube full of MACS Tissue Storage solution The cell suspension was loaded onto the Rhapsody™ Cartridge (BD) to generate single-cell magnetic beads in the microwells according to the manufacturer's protocol. Captured cells were lysed and the released RNA were barcoded through reverse transcription in individual microwells.     The cDNA was generated and then amplified, the quality assessed using an Agilent 4200 (performed by CapitalBio Technology, Beijing). Whole transcriptome libraries were prepared using the BD Resolve single cell whole transcriptome amplification workflow. The libraries were sequenced using an Illumina Novaseq6000 sequencer with a sequencing depth of at least 50,000 reads per cell with pair-end 150 bp (PE150) reading strategy