title: Genomic library of Paenibacillus sp. WG02
design: We first isolated the microbes by suspending the pegmetites in phosphate-buffered saline (PBS) and by applying to LB (WG01) or to R2A (WG02) agar medium incubated at 20C. Single colony was then picked and incubated in LB liquid medium for 48 hours and stored as a glycerol stock. Subsequently, a single colony was again picked and incubated at 30C for 10 hours in LB liquid medium. Subsequently, genome DNA was extracted using NucleoBond HMW DNA kit (Macherey-Nagel, Dren, Germany) according to the manufacturers protocol. Long read sequencing libraries were prepared using Rapid Barcoding Kit (SQK-RBK114.24, Oxford Nanopore Technologies) and sequenced on the GridION device using R10.4.1 Flowcell (FLO-MIN114, Oxford Nanopore Technologies). Sequenced reads were basecalled, demultiplexed, and trimmed for the adaptor sequences using Guppy 6.5.7 (super accurate mode).