title: Illumina NovaSeq 6000 paired-end Sequencing of KAS23051982 (KAE23573880)
design: To construct cDNA libraries with the TruSeq Stranded mRNA LT Sample Prep Kit, total RNA was used. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100nt paired-end sequencing by Illumina NovaSeq 6000. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2100 Bioanalyzer.
library_construction_protocol: TruSeq Stranded mRNA LT Sample Prep Kit