title: Bulk community RNA-Seq sample (53-210 micron size fraction) on January 20th, 2023 from RES2.5 100-200 m
design: Mixed zooplankton samples were size fractionated using 210 and 50 m mesh sieves. After seawater removal, samples preserved in DNA/RNA Shield (Zymo catalog # R1100) were stored initially at -40C and then moved to -80C, prior to DNA/RNA extraction (Quick-DNA/RNA Miniprep Plus Kit, Zymo catalog # D7003). Extracted RNA samples were shipped on dry ice to the sequencing facility (Genomics & Cell Characterization Core Facility [GC3F] at the University of Oregon. Poly-A selected gene libraries were generated using the NEBNext Poly(A) mRNA Magnetic Isolation Module, multiplexed and sequenced using a single lane of Illumina NovaSeq 6000 SP (paired-end sequencing, 2x150 bp).