title: metagenomic analysis of wood samples
design: Fungal communities were analysed using meta rDNA amplicon sequencing. PCR was performed using 30 ng of DNA template and specific primers for the ITS1 region. PCR products were purified with Agencourt AMPure XP beads (Beckman Coulter Life Sciences, US), eluted in elution buffer, and subsequently labelled to complete library construction. Library size and concentration were assessed using the Agilent 2100 Bioanalyzer (Aligent, US). Libraries meeting quality criteria were multiplexed and sequenced on the DNBSeq platform according to their insert size