title: GSM8652566: Muscle, HFD, Control (Bmal1fx/fx;Vhlfx/fx), Animal 5; Mus musculus; RNA-Seq
library_construction_protocol: TRIzol was added to snap frozen whole gastrocnemius muscle and was homogenized using the Qiagen TissueLyser. Following homogenization, RNA was extracted using the Direct-zol RNA Kit (Zymo Research). 300ng of RNA was used for library construction. Libraries for RNA-seq were prepared using the Lexogen QuantSeq 3' mRNA-seq FWD Kit for Illuming sequencing according to manufacturer's protocols.