title: 16S rRNA sequencing of bacterial communities: tracheal aspirates from trachesotomy patients
design: FL-16S (27f - 1492r) primers were utilized for amplification. Oxford Nanopore Technologies SQK-NBD114 native barcoding kit for library preparation of the PCR products. Sequencing was performed on the PromethION 2 Solo platform using a FLO-PRO114M Flow Cell (R10 version). Sequencing was performed with a translocation speed of 400bps. Base calling was performed on the GridION using the super-accurate basecalling model with barcode trimming enabled.   MinKNOW version: 24.06.10 Dorado version: 7.4.19