title: ATAC-Seq analysis of Wt mouse Ly6C-low blood monocytes
design: ATAC-seq was carried out as described earlier with minor modifications (Buenrostro et al. Nature Methods, 2013). A total of 20,000 cells was sorted in ice-cold PBS. Nuclei were isolated with ATAC lysis buffer (10 mM Tris-HCl [pH7.4], 10 mM NaCl, 3 mM MgCl2, 0.1% IGEPAL) and were used for tagmentation, using Nextera DNA Library Preparation Kit (Illumina). After tagmentation, DNA was purified with MinElute PCR Purification Kit (QIAGEN). Tagmented DNA was amplified with Kapa Hifi HotStart Kit (Kapa Biosystems) using 9 PCR cycles. Amplified libraries were purified again with MinElute PCR Purification Kit. Fragment distribution of libraries was assessed with an Agilent Bioanalyzer, and libraries were sequenced on a HiSeq 2500 platform.