title: RNA-Seq of mus musculus: 15-week-old female Hdac4 V275E knock-in mice (wild-type, heterozygous, and homozygous)
design: Total RNA was extracted from paraspinal muscle, spinal cord, and vertebrae of 15-week-old female Hdac4 V275E knock-in mice (wild-type, heterozygous, and homozygous) using TRIzol reagent. RNA quality was assessed using a Bioanalyzer. PolyA-enriched mRNA libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit according to the manufacturers instructions. After library construction, preliminary quantification was performed using a Qubit 2.0 Fluorometer, and libraries were diluted to 1.5 ng/L. The insert size was examined using an Agilent 2100 Bioanalyzer. Libraries with the expected insert size were accurately quantified by qRT-PCR, and only those with an effective concentration greater than 1.5 nM were used for sequencing. Sequencing was conducted on an Illumina NovaSeq X Plus platform with paired-end 150 bp reads.