title: GBS seq of Elymus cognata
design: DNA was digested with the CHG methylation sensitive restriction enzymes PstI and MspI and ligated to the sequencing adapters using T4 DNA ligase. The ligation products were deactivated in 96 well plate then pooled into a single library. The pooled DNA was used as template for PCR with a short extension time of 30 sec to enrich the library.