title: GSM9564227: 10U-L3-P4-LD; Respiratory syncytial virus; RNA-Seq
library_construction_protocol: Total RNA was extracted from virus containing supernatants with Trizol-LS according to the manufacturer's instructions. Figure 3: All WT and R2-10U samples: Library construction proceeded with 200ng of total RNA using the TruSeq Stranded Total Sample Preparation kit (Illumina) with Ribo-Zero ribosomal RNA depletion. Figure 6 and 7: WT< R2-10U and R2-8U sequencing was performed on a NovaSeq X Plus sequencer (Illumina) generating paired-end reads giving ~20 million reads per end.