title: GSM9252750: S_Xnr_2.5; Xenopus laevis; RNA-Seq
library_construction_protocol: RNA was extracted using the acid-guanidinium thiocyanate phenol chloroform method (Chomczynski and Sacchi, 1987) and cDNA was synthesised using the RevertAid reverse transcriptase kit (ThermoFisher). The Xenopus RNA samples were converted to cDNA libraries using a Bioo NextFlex directional v.2 kit and were sequenced at Bristol University's sequencing facility. The human RNA samples were converted to cDNA libraries using Illumina Truseq stranded mRNA library kit and sequenced at the Genome Research Hub, School of Biosciences, Cardiff University.