title: GSM9528686: SNB19 TES-expressing, biological replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from SNB19 cells using the TRIzol reagent isolation system (Sigma-Aldrich) according to manufacturer instructions. RNA quantity and quality were assessed using a TapeStation instrument (Agilent Technologies), and only samples with RNA integrity number (RIN) > 8 were used for library preparation. RNA-seq libraries were generated from 1 ug of total RNA. RNA quantity and quality were assessed using a TapeStation instrument (Agilent Technologies), and only samples with an RNA integrity number (RIN) > 8 were used. RNA was processed according to the TruSeq Stranded mRNA Library Prep Kit protocol (Illumina), following the manufacturer instructions. Libraries were quantified, pooled, and sequenced on an Illumina HiSeq 3000 platform to generate 76-bp stranded paired-end reads using Illumina TruSeq technology.