title: GSM8790285: 19-1XX-preinfusion rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Trizol Two ng RNA with integrity numbers ranging from 9.1 to 9.3 underwent amplification using the SMART-seq v4 Ultra Low Input RNA Kit (63488, Clonetech) with 12 cycles of amplification: 95°C for 60 s, 12 times (98°C for 10 s, 65°C for 30 s, 68°C for 180 s) and 72°C for 10 min. Subsequently, 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (KK8504, Kapa Biosystems) using 8 cycles of PCR. The final DNA library was double-size purified (0.8-1x) with Ampure XP beads (Beckman coulter)