title: RNAseq of Arabidopsis germinating seeds
design: RNA was isolated from 50 mg dry material with 2 ml of polysome extraction buffer. 50-L of supernatant was used to extract total RNA by TriPure Isolation Reagent (Roche, Basel, Switzerland), followed by clean-up using RNeasy Mini spin columns (Qiagen, Hilden, Germany). 5 g of total RNA were subjected to rRNA depletion using Illumina Ribo-Zero Plus rRNA Depletion Kit following the manufacturers instructions. The rRNA-depleted RNA was used to construct sequencing libraries using the ARTseq/TruSeq Ribo Profile Kit (Illumina).