title: MPO977
design: High-molecular-weight DNA (2 g) was fragmented using a G-TUBE, followed by DNA damage repair and A-tailing using the NEBNext FFPE DNA Repair Mix and the NEBNext Ultra II End Repair/dA-Tailing Module. Barcode sequences were added, and sequencing adapters were ligated using the Non-Amplification Barcode Extension Kits 1-12 or 13-24