title: Amplicon-Seq of Homo sapiens: HEK293T cell line; multiple targeted loci; tool=ZF-V, V3; rep2 (PE150)
design: Genomic DNA was extracted from HEK293T. ~250 bp regions flanking CRISPR-edited loci were PCR-amplified with locus-specific primers, pooled per sample, purified, and indexed by a second PCR to add Illumina adapters and unique dual indexes. Libraries were quantified, normalized, and sequenced by GENEWIZ (Azenta) on an Illumina platform using paired-end 150 bp (PE150). Data are used to quantify editing efficiency at targeted loci.