title: GSM8545660: D, rep5; Homo sapiens; RNA-Seq
library_construction_protocol: Naïve (CD19+ CD20+ IgD+ CD27- CXCR5+ CD11c-), DN1 (CD19+ CD20+ IgD- CD27- CXCR5+ CD11c-), and switched memory (CD19+ CD20+ IgD- CD27+ CXCR5+ CD11c-) B cells were FACS purified from cryopreserved PBMC's using a FACS Aria Fusion cell sorter. Total RNA was isolated from sorted B cell populations using the RNAeasy Plus Micro kit (Qiagen) according to the manufacturer's instructions. RNA quality was assessed via Agilent 4200 Tapestation and RNA concentration was determined using a Qubit Flex (Fisher Scientific). Total RNA (4 ng/sample) was processed using the SMARTer Stranded Total RNA-seq Kit v3-Pico Input Mammalian (Takara) to create RNA-seq libraries.