title: RNAseq of Breast Implanted ALK-negative anaplastic large cell lymphoma cell line (TLBR2) upon silencing of a novel lncRNA specifically associated with the  ALK-negative anaplastic large cell lymphomas.
design: RNAseq of Breast Implanted ALK-negative anaplastic large cell lymphoma cell line (TLBR2) upon silencing of a novel lncRNA specifically associated with the  ALK-negative anaplastic large cell lymphomas.
library_construction_protocol: Cells were collected after 24 hours from lncMAAT silencing. Cells were cultured in RPMI-1640 medium supplemented with 10% FBS and 20U/ml IL2. TLBR-2 cells were transfected with 50nM Antisense LNA GapmeRs concentration. Antisense LNA GapmeR transfections was performed using the Cell Line Nucleofector Kit SF and Amaxa 4D Nucleofector (program DS-130). 24 hours after transfection, cells were harvested for RNA extraction. Antisense LNA GapmeR Negative Control (Cat. No. / ID: 33951, Qiagen) was used as negative control. Total RNA from cells was extracted by TRIzol (Thermo Fisher Scientific) according to the manufactureâ€™s instructions. Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.