title: GSM9204879: Colon Tumor from injected AKPS SI tumor Organoid Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: For tissues, pieces of tumor or intestinal tissue were sampled from mice, placed in RNAlater and stored at -80°C before extraction. For organoids, confluent organoid samples were grown and a cell pellet was snapped frozen on dry ice. RNA was extracted from samples using the RNeasy Mini Kit (Qiagen) following manufacturer's instructions. For tissues, homogenisation was done by using Precellys ceramic bead filled tubes in a Precellys Evolution machine (Bertin Instruments). For organoid pellets, cells were dissociated using pipetting in the 1st step of the extraction protocol. Libraries for cluster generation and DNA sequencing were prepared using an Illumina TruSeq RNA LT kit, then run on an Illumina NextSeq 500 (2x36 cycles, paired end, single index).