title: GSM9204136: Somatosensory Cortex, Female, P7, KO, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: From each mouse the somatosensory cortex, the cerebellum and the hippocampus were collected. Tissues were homogenized in TRIzol Reagent (Thermo Fisher Scientific); total RNA was extracted with the RNeasy Mini Kit. (Qiagen, no. 74104). A total of 500 ng of RNA was used for sequencing. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library prep (Illumina, catalog number 20020595) in accordance with manufacturer recommendations, and using IDT for Illumina TruSeq DNA UD Indices (Illumina, catalog number 20022370) for adapters.