title: CK-3
design: The aim of the experiment was to investigate the effect of trpE gene on A. salmonicida.  To understand transcriptomic changes in the absence of trpE, we will employ Illumina NovaSeq 6000 platform for deep transcriptome sequencing. By comparing RNA data from the control group and the experimental group, the aim was to reveal key gene expression patterns affected by pollutants and their potential functions. Samples: The experiment utilizes two sets of cultures: one set is the wild-type A. salmonicida;  the second set is A. salmonicida trpE deletion strain.  Both strains are cultivated under identical conditions to ensure environmental consistency affects expression minimally.