title: GSM9205001: Germinal center B cells (CD19+IgD-CD95+CD38-) isolated from mice immunised with NP-KLH (D7) (H3K27me3 ChIP); Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed in 0.6% formaldehyde for 8 minutes, while rotating, before quenching with 1.25M glycine and washed thrice with Dulbecco's phosphate buffered saline. Cell were lysed via a BioRuptor sonicator. Histone modification-specific antibodies were used to pull down DNA fragments via chromatin immunoprecipitation and input sampels representing the fragmented DNA prior to pulldown were generated for each sample. ChIP-processed and input control samples were processed using the MGI Easy DNA FS Library preparation kit V1 (MGI Tech) and sequenced using a MGISEQ-2000RS High-Throughput Sequencing Set (PE100).