title: GRBV Epigenetics Asymptomatic Replicate 2
design: An epigenomics sequencing library was prepared using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) following the ligation sequencing amplicons protocol. Linear double-stranded GRBV DNA (200 fmol), obtained from circular ssDNA, was used from asymptomatic greenhouse samples (three biological replicates). DNA ends were repaired with the NEBNext Ultra II End Repair/dA-Tailing Module, and barcodes were ligated using NEB Blunt/TA Ligase Master Mix. AMPure XP beads (1.5X) were used for cleanup before pooling the barcoded samples. Sequencing adapters were then ligated using the NEBNext Quick Ligation Module, followed by another bead cleanup. The final library was quantified with the Qubit 4 Fluorometer and the Qubit 1 dsDNA HS Assay Kit.