title: GSM8602325: PHK-Ctrl-IgG4; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen RNeasy Mini Kit Poly (A) mRNA was subsequently extracted and enriched from total RNA utilizing oligo (dT)-attached magnetic beads according to the manufacturer's (Illumina) instructions. Enriched and purified mRNA was fragmented into approximately 200 nt short mRNA, followed by first-strand cDNA synthesis using random hexamers as primers. Second-strand cDNA was constructed in a buffer containing dNTPs, DNA polymerase I and RNaseH. Appropriate fragments were isolated and enriched by PCR amplification.