title: GSM9075398: 1836_2023; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol reagent and further purified/Dnase treated with the RNeasy Mini Kit cleanup protocol (Qiagen, Hilden, Germany). mRNA was isolated from 1.1 μg of total RNA and used to generate dual-indexed cDNA libraries with the Illumina stranded mRNA ligation kit (Illumina) and UD Indexes Set A (Illumina). Ten cycles of PCR amplification were applied to all libraries. Sequencing-ready libraries were quantified using the Qubit dsDNA HS assay (Invitrogen) and quality controlled with the Bioanalyzer 2100 DNA HS assay (Agilent). An equimolar pool was prepared with the six libraries and submitted for sequencing on a NovaSeq6000 S4 (Illumina). More than 71 Gbp were produced, with a minimum of 36.5 million of paired-end reads per sample.