title: Hi-C chromatin interaction mapping of Hishimonus sellatus
design: A Hi-C library was constructed to assist in chromosome-level scaffolding of the genome assembly. Fresh tissue samples were crosslinked with formaldehyde to fix chromatin interactions in situ. The crosslinked DNA was digested with a restriction enzyme, followed by biotin filling of the overhangs and proximity ligation to join interacting DNA fragments. After reversing the crosslinks, DNA was purified and sheared. Biotinylated fragments were enriched using streptavidin beads. The resulting DNA was used to prepare a standard Illumina paired-end library, which was sequenced on the Illumina NovaSeq 6000 platform to generate paired-end reads, capturing long-range genomic interactions.