title: Whole genome sequencing of Trichoderma asperellum BRFT7
design: Nanopore whole genome sequencing (WGS) library preparation is a ligation-based, workflow optimized for high molecular weight genomic DNA. The protocol begins with DNA repair and end-preparation to generate blunt, dA-tailed ends suitable for ligation.Unique native barcodes can be attached to each sample, enabling multiplexing without amplification and preserving native DNA modifications. Sequencing adapters are then ligated, and the library is purified using magnetic beads. The final quantified library is loaded onto an R10.4.1 flow cell.