title: miRNA Expression Profiles in Burkitt and Hodgkin lymphoma cell lines upon Ionizing Radiation Exposure
design: miRNA Expression Profiles in Burkitt and Hodgkin lymphoma cell lines upon Ionizing Radiation Exposure
library_construction_protocol: Burkitt lymphoma and Hodgkin lymphoma cell lines were exposed to ionizing radiation at a dose of 4 Gy and collected 1h, 4h and 12h upon irradiation, control unirradiated cells were collected at 4h timepoint.  Total RNA was extracted using the miRNeasy Mini Kit  (QIAGEN, Cat. No. 1038703). This kit is specifically designed for the isolation of high-quality RNA, including small RNAs such as miRNA, from a variety of sample types. After addition of chloroform, Heavy 5PRIME Phase Lock GelTM (QuantaBio, Cat. No. 2302830) was applied to improve organic/inorganic phase separation. Quality control of RNA was performed using Bioanalyzer. A gel-free, bead-based size selection procedure was applied to isolate RNA fragments ranging between 15 and 40 nucleotides. Libraries were generated with Illumina QIAseq miRNA Library Kit and integrated UMI technology was applied.