title: RNA-seq (NEBNext Ultra RNA-seq), nlp7 mutant, leaf protoplasts, transfected with empty vector control, replicate 1
design: Total RNA was extracted from Arabidopsis nlp7 mutant  leaf protoplasts (22-day-old plants). Protoplasts were transiently transfected with either NLP7 construct (pBOB11-NLP7-GFP) or empty vector control, treated with CHX (35 M, 20 min) and DEX (10 M, 3 h). GFP-positive cells were isolated by FACS. RNA-seq libraries were prepared with the NEBNext Ultra RNA Library Prep Kit for Illumina and sequenced on an Illumina HiScanSQ platform.