title: Dovetail OmniC sequencing data for Charadrius nivosus
design: The Omni-C library was prepared using the DovetailTM Omni-CTM Kit (Dovetail Genomics, Scotts Valley, CA) according to the manufacturers protocol with slight modifications. First, specimen tissue (liver, ID: PMB2274) was thoroughly ground with a mortar and pestle while cooled with liquid nitrogen. Subsequently, chromatin was fixed in place in the nucleus. The suspended chromatin solution was then passed through 100 m and 40 m cell strainers to remove large debris. Fixed chromatin was digested under various conditions of DNase I until a suitable fragment length distribution of DNA molecules was obtained. Chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA was purified from proteins. Purified DNA was treated to remove biotin that was not internal to ligated fragments. An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor. Biotin-containing fragments were then captured using streptavidin beads. The post capture product was split into two replicates prior to PCR enrichment to preserve library complexity with each replicate receiving unique dual indices. Sequencing was performed at the Center for Advanced Technologies at the University of California, San Francisco (UCSF CAT) on an Illumina NovaSeq X platform (Illumina, CA) to generate approximately 100 million 2 x 150 bp read pairs per GB genome size.