title: GSM9253425: rna_cdca7acdca7b_rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: 14-day old seedlings were harvested from Col-0, cdca7a, cdca7b, cdca7a cdca7b+/-, cdca7a cdca7b, h1.1 h1.2, h1.1 h1.2 cdca7a cdca7b, and ddm1-2. Samples were immediately frozen in liquid nitrogen. DNA were extracted using the DNeasy Plant Mini kit (Qiagen). RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo Research). 500ng of total RNA from seedling tissues were used for RNA-seq library preparation with TruSeq Stranded mRNA kit (Illumina). The final library was sequenced on Illumina Novaseq X plus instruments.