title: GSM9564402: PC9-Cas9 cells, Untreated, replicate 1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was isolated using the Wizard Genomic DNA Purification Kit (Promega). Sequencing libraries were generated using 2 sequential PCR reactions. The first PCR amplified the sgRNAs from genomic DNA, and the second PCR added multiplexing barcodes.