title: GSM8599802: KO3_GAS; Mus musculus; RNA-Seq
library_construction_protocol: RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) After fragmentation of the mRNA, the first-strand cDNA is synthesized using random hexamer primers, followed by the synthesis of the second-strand cDNA. After end repair, A-tailing, adapter ligation, fragment selection, amplification, and purification, the library is ready