title: RNA-Seq of Maruca vitrata: Suseptiable sample 2
design: RNA was isolated from the 4th instar larvae of insecticide susceptible and resistant populations of Maruca vitrata NEBNext Poly (A) mRNA magnetic isolation module (New England Biolabs) by following the manufacturers protocol. The enriched mRNAs were further taken for the library preparation using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). RNA from three biological replicates of each population was prepared and RNA concentration was measured by Qubit dsDNA HS (High Sensitivity) Assay Kit (ThermoFisher  Scientific) in QubitTM 3.0 fluorometer (Thermo Fisher Scientific, USA) and the integrity was assessed by using Agilent D1000 ScreenTape System (Agilent) in a 4150 TapeStation System (Agilent Technologies, USA).  For each sample, 1l of the purified library was mixed with 3l of D1000 sample buffer and vortexed using IKA vortexer at 2000rpm for 1min and spun down to collect the sample to the bottom of the strip. The strip was then loaded on the Agilent 4150 TapeStation system. The qualified libraries were sequenced on Illuminas HiSeq 2500 sequencer (Clevergene, India) and 150 bp paired-end reads were obtained with an average coverage of 150X. Approximately 6 GB data was obtained for each of the samples.