title: RNA-Seq of Artemisia ludoviciana: leaf
design: Total RNA was extracted from fresh plant samples using Quick-RNA Plant Miniprep Kit. The integrity of the isolated total RNA was assessed using a Qsep100 bio-fragment analyzer, and the quality and quantity were evaluated with a NanoDrop 2000 spectrophotometer and a Qubit 4 fluorometer, respectively. The RNA-seq library was constructed using the TrueSeq Stranded Total RNA kit. The rRNA was removed from the total RNA sample using the Ribo-Zero Plant depletion system. The quantity and quality of the cDNA library were evaluated using a Qsep100 bio-fragment analyzer. The cDNA library was sequenced using the Illumina NextSeq 550 platform in a paired-end 150 base run.