title: Exome sequencing results for sample s_C_DEMAKC_N001_d
design: DNA isolation proceeded with the DNeasy Blood & Tissue Kit (QIAGEN catalog # 69504). DNA was eluted in 0.5X Buffer AE heated to 55°C. Nucleic acids were subsequently extracted using the AllPrep DNA/RNA Mini Kit (QIAGEN catalog # 80204) according to the manufacturer's instructions. Exome sequencing libraries were made following PicoGreen quantification and quality control by Agilent BioAnalyzer, 57-500ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. After sample barcoding, 100-500ng of library were captured by hybridization using the xGen Exome Research Panel v1.0 (IDT) according to the manufacturers' protocols. PCR amplification of the post-capture libraries was carried out for 8 cycles. Samples were run on a HiSeq 4000 in a PE100 run, using the HiSeq 3000/4000 SBS Kit (Illumina). Normal and tumor samples were covered to an average of 97X and 164X, respectively.