title: Root transcriptomic response of three potato cultivars (Desirée, Innovator, Mozart) to salt stress (NaCl=125mM) after 6h and 24h
design: Root transcriptomic response of three potato cultivars (Desirée, Innovator, Mozart) to salt stress (NaCl=125mM) after 6h and 24h
library_construction_protocol: Roots were collected at ZT10 (time point 6h) and the following day at ZT4 (time point 24h). Three biological replicates were used and each biological replicate is composed of the roots of three plants. Tissues were flash-frozen in liquid nitrogen and subsequently ground with a mortar.  Plants were grown in hydroponic conditions (0.5MS, 0.025mM MES). Plants were grown in a growth chamber with a 16h light / 8h dark light cycle.  NaCl was added to the hydroponic tanks to a final concentration of 125mM  RNA was extracted with FavorPrep™ Plant Total RNA Mini Kit from FAVORGEN. DNA was degraded in the column with RNase-free DNase I (Roche).  RNA library preparation was conducted by Novogene Co., LTD (Cambridge, United Kingdom)