title: Transcriptome capture RNA-Seq of human melanoma (primary tumor)
design: Libraries were generated from total RNA using  Illumina's TruSeq RNA Access Library Prep Kit. Synthesis of the second strand of cDNA was followed by indexed adapter ligation. Subsequent PCR amplification enriched for adapted fragments. The resultant cDNA libraries were hybridized to capture probes targeting the exome and blocking oligos to reduce off-target binding. Streptavidin magnetic beads were used to capture the library-bound probes, followed by washing steps. After enrichment the libraries were quantified and then pooled equimolarly.  All library construction steps were automated on a liquid handling system.