title: GSM9636528: 4D NMP, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: NMP cultures subjected to bulk-RNA sequencing were first washed with 1X DPBS to remove the media. Cells were then lysed by directly adding 600μL of RLT buffer in the culture wells, and the lysate is then collected and flash frozen with liquid nitrogen to be stored at -80ºC. The total RNA was then purified using Qiagen's RNeasy kit, and eluted in 50μL of RNAse-free water. Using a microvolume spectrophotometer, RNA concentrations were determined and diluted to 1μg/μL. RNA samples were further subjected to quality control assessment using Agilent Technologies 2100 Bioanalyzer and only samples with a RIN score >8.0 were used for library construction using Universal plus mRNA sequencing kit (NuGEN).