title: RNA-Seq of mus musculus primary cortical astrocytes: EVs from infected neurons, replicate 3
design: Primary astrocytes were plated at 1x10^5 cells per well in 2 mL in 6 well plates. One day after plating 2 uL of Dexamethasone (100 nM) was added to each well. One day after Dexamethasone treatment EVs from infected neurons (2 ug/mL) were added and incubated for 24 hours. Following incubation samples were lysed with the Qiagen RNeasy Mini Kit according to the manufacturer's protocol.