title: Shotgun metagenomics: female adult gut Diaprepes abbreviatus
design: DNA was extracted from full guts using the ZymoBIOMICS-96 MagBead DNA kit, following manufacturers instructions. Libraries were then prepared using the Illumina DNA Library Prep Kit (Illumina, San Diego, CA) following manufacturers instructions using unique dual-index 10 bp barcodes with Nextera adapters (Illumina, San Diego, CA). Libraries were then pooled in equal abundance, and the final pool was quantified using qPCR and TapeStation (Agilent Technologies, Santa Clara, CA), and sequenced using NovaSeq X (Illumina, San Diego, CA). The ZymoBIOMICS Microbial Community Standard (Zymo Research, Irvine, CA) was used as a positive control for each DNA extraction and for each library preparation. Negative controls, including a blank extraction control and a blank library preparation control, were added to assess cross-contamination and bioburden.