title: CRISPRi-seq of Streptococcus pneumoniae in zebrafish embryo meningitis model: doxycyline treatment
design: A doxycycline-inducible S. pneumoniae D39V CRISPRi library was used in a zebrafish embryo meningitis model treated with doxycycline, after which bacteria were collected from whole animals followed by genomic DNA isolation, amplicon library construction and sequencing