title: Holstein dairy cow
design: total RNA was isolated using the Trizol Reagent (Thermo Fisher Scientific Inc, U.S.A), after which the concentration, quality, and integrity were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific Inc, U.S.A). Three micrograms of RNA were used as input material for the RNA sample preparations and sequencing libraries were generated. The sequencing library was then sequenced on NovaSeq 6013 platform (Illumina).