title: GSM8716294: HUVEC cells exposed to 14 µg/ml bleomycin for 24h, replicate 4; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cells after 24h, 48h, and 72h of exposure using the RNeasy Mini kit (Qiagen). DNase 1 (Thermo Scientific, EN0521) treatment was used prior to downstream analyses. RNA-Seq libraries were prepared using the Novogene NGS RNA Library Prep Set (PT042) including polyA-tailed mRNA enrichment. RNA-Seq was performed in two batches i.e., the two exposure experiments of bleomycin and TGF-beta, respectively.