title: Whole-Genome Sequencing of Levilactobacillus brevis YT108: Rex Rabbit Feces
design: De novo bacterial genome sequencing was conducted utilizing next-generation sequencing technologies. For survey sequencing, Illumina's platform was employed to generate 400 base pair fragments from high-quality DNA samples, followed by 150 bp pair-end sequencing to achieve a minimum genome coverage of 100. The process yielded multiple genomic scaffolds. To enhance assembly completeness, finished sequencing was performed using a combination of Illumina and PacBio platforms, each providing 100 coverage. This strategy ensures the inclusion of all genetic elements, including small plasmids (<15 kb), to obtain a comprehensive genome sequence.