title: Illumina whole genome resequencing of Hishimonus sellatus
design: Whole genome resequencing was performed using the Illumina platform for genome survey and assembly polishing. Genomic DNA was extracted from the pooled samples and fragmented into a size range of 300-400 bp using ultrasonic shearing. The fragments were end-repaired, A-tailed, and ligated with Illumina sequencing adapters. The adapter-ligated fragments were size-selected and amplified by PCR. The library quality was checked using an Agilent 2100 Bioanalyzer and Q-PCR. The final library was sequenced on an Illumina NovaSeq 6000 system to generate 150 bp paired-end reads.