title: GSM9378301: Mnk3, CRISPRa, scramble, With stimulation, replicate 1, merged; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested by spin column (Qiagen RNeasy kit) for RT-qPCR and RNAseq, in triplicate. Standard polyA selected bulk mRNA seq libraries were generated for Illumina 100bp paired-end sequencing (Illumina platform, University of Cincinnati Genomics, Epigenomics, and Sequencing Core, Cincinnati, OH, USA). RNAseq samples were performed in triplicate.