title: RNAseq of ccRCC cells with GPR132 knockout
design: RNAseq of ccRCC cells with GPR132 knockout
library_construction_protocol: RNA collection cells were grown to 70% confluency prior to harvest cells were infected with pLentiCRIPRv2 encoding gRNA to GPR132 RNA was harvested by Trizol extraction cDNA synthesis by standard oligo dT priming