title: RNAseq of Arabidopsis germinating seeds
design: RNA was isolated from 50 mg dry material with 2 ml of polysome extraction buffer. A 200-L aliquot of supernatant digested with RNase I nuclease. Fragmented RNA was purified and size separated, gel slices from 26 to 34 nt were excised. RNA was recovered from the excised gel slices (Ingolia et al., 2012). Ribo-seq libraries were constructed according to ARTseq/TruSeq Ribo Profile Kit manual.