title: RNA-Seq of HT22: IH-3
design: Total RNA was extracted from brain tissues or cells using the TRIzol reagent(Invitrogen, CA, USA) according to the manufacturers instructions. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturers instructions.The libraries were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated.