title: Whole genome sequencing of Plasmodium knowlesi Knockout mutants not rescued by duplicated loci
design: Parasites cultured in human RBCs. Genomic DNA extracted using the QIAGEN DNA extraction kit. Library prepartion using NEBNext Ultra II DNA Library Prep Kit. Sequencing was performed on the Illumina NextSeq 2000 system using the P2-300 cycles reagent kit.