title: RNA-Seq of Citrus: leaves
design: RNA was extracted using the TRIzol method, and RNA integrity was checked by 1% agarose gel electrophoresis. The extracted total RNA was stored in a 80 C freezer and then transported on dry ice to Xinjiang Aidesen Biotechnology Co., Ltd. (Urumqi, China) for sequencing. The extracted RNA was fragmented in a PCR plate placed in a magnetic plate holder. The fragmented mRNA was reverse transcribed into cDNA using Superscript II and random primers (Invitrogen, Carlsbad, California, USA). Data filtering and quality control were performed using fastp software, and the resulting clean data were used for subsequent analysis [24]. The reads were aligned in HISAT2 using the citrus genome as a reference, and String Tie was used to quantify the aligned reads [25, 26].