title: GSM8579884: RNA_seq_KMS27-002_CN; Homo sapiens; RNA-Seq
library_construction_protocol: KMS27 and KMS18 cell lines were maintained in RPMI1640 medium supplemented with 10% FBS and 1% glutamine. Total RNA was extracted from an equal number of viable cells using QIAzol Lysis Reagent (Qiagen) and purified from DNA contamination through a digestion step with the RNase-Free DNase Set (Qiagen). RNA libraries were generated using an equal amount of RNA from each cell line sample, following the protocol of the Illumina Stranded Total RNA Prep kit, with an initial ribosomial depletion step using Ribo-Zero Plus from Illumina.