title: ="16S amplicon sequencing of biofilm metagenome: "&B13
design: Total genomic DNA was extracted from water and in situ colonized microplastic biofilm samples using commercial DNA isolation kits. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified by PCR using specific primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-14'). The resulting PCR products were purified, pooled, and used to construct amplicon libraries for high-throughput sequencing on the Illumina platform.