title: Aristolochic acid II treatment
library_construction_protocol: Amplified yeast genomic DNA (10 µg DNA, one µg per µl) was treated with 0.25 mM AAII solution in 50 µl of 50 mM potassium phosphate buffer, pH 5.8. AAII was activated by 1 mg of zinc dust and incubated at 37 °C for 30 minutes. AAII-treated DNA was purified by using 1.8x Ampure XP beads and eluted in 25 µl 10 mM Tris-HCl, pH 8.0.                