title: GSM9460836: CONDRO_SCB_LPS_4_L1_R1_001_mQ6i5Qk9l264.fastq.gz; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was isolated from primary chondrocytes using the miRNeasy Micro Kit (Qiagen), following the manufacturer's instructions. To improve ligation and reverse transcription efficiency of modified tRNA fragments, RNA was pre-treated using the rtStar™ tRF&tiRNA Pretreatment Kit RNA library preparation was conductuced by the QIAseq miRNA Library Illumina kit (#331502, Qiagen) accordingly to the manufacturer's protocol