title: The strains extracted from the liver of the black-spotted side-striped frog were purified, cultured, and sequenced
design: Dominant bacteria with the same colony morphology were isolated from the skin, liver and brain tissues and temporarily named HBG0512. A single colony was picked and added to 100 L sterile water to prepare a bacterial suspension. The bacterial genomic DNA was extracted according to the instructions of the bacterial DNA extraction kit (Sangon Biotechnology, Shanghai). The 16SrDNArRNA universal primers (27F: AGAGTTTGATCCTGGCTCAG; 1492R: GGTTACCTTGTTACGACTT) were used for amplification. The amplified products were detected by 1% agarose gel electrophoresis and then sent to Sangon Biotechnology (Shanghai) Co., Ltd. for sequencing.