title: GSM8602349: male, chow diet, sample 1; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq using frozen liver samples were performed using our previous optimized protocol (Zhang, Z. et al. Nat Commun, 2020). The number of nuclei in each sample was counted using Trypan Blue (Thermo Fisher) and a hemocytometer, and 75,000 nuclei were extracted and centrifuged at 500 g for 5 min at 4°C and used for the transposition reaction. ATAC-Seq libraries were constructed using the Nextera Tn5 Transposase and DNA library preparation kit (Illumina) as described (Buenrostro et al., Curr Protoc Mol Biol, 2015). We performed size selection usign AMPure XP magnetic beads.