title: 16S rRNA amplicon sequencing of Hydra microbiome low LPS replicate 1
design: 16S rRNA gene libraries were prepared from the DNA extracts using the 16S Barcoding Kit 1-24 (SQK-16S024) (Oxford Nanopore Technologies, UK) according to the manufacturers instructions. Nuclease-free water (Omega Bio-tek, Inc., US) was used as a negative control, and ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, US) was used as a positive control. Xpert ampliFi Hotstart 2X Mastermix (GRiSP Lda., PT) was used for amplification. The libraries were purified using AMPure XP beads (Beckman Coulter, Inc., US).