title: GSM9252217: Ctrl-RNA-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA of control group was extracted using TRIzol Reagent (Invitrogen) following the manufacturer's protocol. RNA was precipitated with isopropanol, washed with 75% ethanol, and resuspended in RNase-free water. RNA of Co-extraction group was isolated using the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) according to the manufacturer's instructions, ensuring compatibility with subsequent proteomic and phosphoproteomic workflows. RNA libraries were constructed using T7-based in vitro transcription amplification following the manufacturer's protocol. Sequencing was performed on an Illumina NovaSeq 6000 platform with 150-bp paired-end reads (PE150).