title: Oxford Nanopore sequencing of Solarion arienae genome
design: Four sequencing libraries were prepared using the Ligation Sequencing Kit (SQK-LSK109) from Oxford Nanopore Technologies, following the manufacturers protocol for sequencing genomic DNA by ligation. The libraries were loaded onto two R9.4.1 FLO-MIN106 Spot-On Flow cells and run on a MinION Mk1B device for 24 (1st run) and 48 (2nd run) hours in the software MinKNOW v21.06.0. Three additional duplex sequencing libraries were prepared with the Ligation Sequencing Kit (SQK-LSK109) and the Native Barcoding Expansion 1-12 EXP-NBD104 following the manufacturers protocol for barcoding genomic DNA samples in multiplex libraries. The libraries were loaded onto FLO-MIN106 Flow cells and sequenced as described above. The raw data from singleplex libraries were basecalled using the Super accurate (SUP) model of the Guppy GPU software v6.0.1 with chimera detection and adapter trimming parameters: --do_read_splitting --max_read_split_depth 3 --trim_adapters. The data from the multiplex libraries were demultiplexed with additional parameters for barcoding: --barcode_kits EXP-NBD104 --detect_mid_strand_barcodes --trim_barcodes.