title: whole genome CRISPR Screening (Brunello library) on AMO-1 cell line treated with Ceritnib
design: whole genome CRISPR Screening (Brunello library) on AMO-1 cell line treated with Ceritnib
library_construction_protocol: Cells were harvested and DNA was isolated and stored in -80 degree DNA was isolated using Qiagen Blood & Cell Culture DNA Kits 500/g cca 50-60-mio cells PCR libraries were prepared according to established protocol and primers published here doi: 10.1158/0008-5472.CAN-20-3323.