title: Element sequencing of HiC library of adult male whole body pool Quadrastichus erythrinae USDA-ARS-TPGMBRU Wild
design: Tissue from 12 adult males was cross-linked, digested using the enzymes DdeI and DpnII, and proximity ligated. After proximity ligation and nucleic acid purification, the sample was size-selected to enrich for DNA fragments 200-600 bp. Following sample purification using SPRI-beads, library preparation was performed using the NEB Next Ultra II DNA Library Prep Kit. The resulting HiC library was sequenced on one 2 x 150 Cloudbreak FS Sequencing Kit on an Element Biosciences Aviti system to approximately 400,000 proximity ligations.