title: GSM9253510: Lesional UD skin, scratching-induced, TG; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was manually and enzymatically dissociated in dispase and then in accutase and collagenase at 37°C. Cell suspensions were filtered with 40 µm cell strainers, washed, and counted prior to capture. Single cell suspension was captured using the 10x Genomics Chromium GEM-X Single Cell 5' v3 kit. Cell suspensions were loaded into a GEM-X chip along with partitioning oil and gel beads. Library construction was performed according to the manufacturer's specifications (User Guide CG000733) for the 5' v3 chemistry.