title: DNA reseq of Roman S. pilchardus
design: Double stranded DNA Illumina libraries were built from the extracts. A blunt-end library was constructed on 21.25 l of DNA extract following the protocols outlined by Maricic et al. (2010) and Meyer and Kircher (2010). The amplified libraries were run on Agilent 2100 Bioanalyzer High Sensitivity DNA chip. The sequencing libraries were pooled with other libraries and sequenced in one lane on a llumina HiSeq 2000 (100 cycles, single read mode) at Novogene and SciLife Lab.