title: CUT&RUN data of Chd4 and Mbd3 in mouse embryonic stem cells
design: CUT&RUN data of Chd4 and Mbd3 in mouse embryonic stem cells
library_construction_protocol: Cells were cultured on 10cm plates. Cells from all conditions were harvested at the same time using accutase and spun down for 3 min, 600 x g in a swinging bucket rotor at room temperature. Cells were washed in PBS and processed immediately. ES cells were cultured on 0.1% gelatin-coated plates in 2i/LIF conditions. DNA was extracted using Zymo DNA Clean and Concentrator Kit-5 (Cat. No. D4014). Library preparation for sequencing was carried out in the SCI facilities. 