title: RNA-seq of yolk from an infected live embryo
design: Ribosomal RNA (rRNA) depletion targeting both eukaryotic and prokaryotic rRNAs using the Illumina Ribo-Zero Gold rRNA Removal Kit (Epidemiology).Strand-specific cDNA libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, USA)