title: GSM9234546: HEK293T-PRPS1-HA-dTAG-PRPSAP1-V5-BromoTag_AGB1_Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Qiagen Rneasy Kit 1ug total RNA was submitted to Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.