title: GSM8601934: e2Merv_dtag_bulk_72hr_aIgG_cnr_rep4; Mus musculus; OTHER
library_construction_protocol: CUT&RUN on MERVL GFP- EHMT dTAG lines were treated with DMSO/dTAG for 72hrs , Sorted for gfp positive and negative population. Cut and Run performed using anti-DPPA4 antibody  was performed on 250k-450k cells per sample using BioMag Plus Concanavalin A beads (ConA beads, PolySciences NC1358578), 1:100 anti-DPPA4  ((AF3730)) or anti-IgG (EpiCypher 130042), and ProteinA/G MNase (EpiCypher). Fragmented DNA was isolated by phenolchloroform extraction and EtOH precipitation CUT&RUN libraries were constructed using the Takara Thruplex Library Prep and Unique Dual Index kits. Cycles for PCR amplification were determined individually for each sample by qPCR. Libraries were size selected using SPRI beads (Beckman Coulter)