title: RNA-seq experiment of multiple myeloma cells under different treatment conditions against epigenetic modifiers
design: RNA-seq experiment of multiple myeloma cells under different treatment conditions against epigenetic modifiers
library_construction_protocol: INA-6, OPM2 and KMS-28PE were selected for combinatorial treatment response analysis. INA-6 and KMS28-PE cells were seeded at 100.000 cells/ml while OPM2 cells were seeded at 200.000 cells/mL 24h prior treatment with H2O and DMSO (Sigma-Aldrich) or 50nM of AZA (Abcam) and 5μM or 10μM of A366 (Tocris Bio-techne). AZA treatment was administrated every day while A366 treatment was added every 3 days. Media and reagent refreshment was performed every 3 days. All experiments were performed in three independent cell batches per cell line. RNA was extracted using the RNeasy Micro Kit (Qiagen; Hilden; Germany #74004) as described by the manufacturer. Sequencing libraries were prepared from 100ng total RNA using the Illumina Stranded Total RNA library preparation kit with Ribo-Zero Plus treatment (cat# 20040525/20040529, Illumina Inc.)