title: RNA-Seq of mus musculus: adult female tumor
design: Total RNA was extracted using TRIzol, and RNA quality was assessed with an Agilent Bioanalyzer and agarose gel electrophoresis. Eukaryotic mRNA was enriched with oligo(dT) beads, fragmented, and reverse transcribed into cDNA. The cDNA was end-repaired, A-tailed, adapter-ligated, purified, size-selected, and PCR-amplified to create sequencing libraries.