title: scRNA-seq of Mus musculus: tamoxifen AIDCreERT2xR26eYFP mice
design: A single Peyer's patch (PP) of an AIDCreERT2xR26eYFP mouse was injected with tamoxifen at day 0. Immune cells of the injected PP and the two adjacent PPs  were isolated 14 days later. Cells from each PP were hashtagged with Feature Barcode technology (TotalSeq C #1-#3, BioLegend).  Non-naive and naive B cells were FACS sorted using the FACSAria Fusion (BD) equipped with a 70 mm Nozzle.  Cells were further processed using the Chromium Next GEM Single Cell 5' Reagent Kit v2 according to the manufacturer's instructions (10x Genomics, Inc., United States). The experiment aimed for 5000 recovered cells. Sequencing was performed on an Illumina NextSeq 500.