title: 16S rRNA Amplicon Sequencing of Rats with Adenine-induced Chronic Kidney Disease
design: DNA was extracted from rats' fecal samples with MOBIO PowerSoil DNA Isolation Kit (12888-100) and stored at -80C in Tris-EDTA buffer solution until use. The V4 region of the 16S rRNA gene was polymerase chain reaction (PCR) amplified using barcoded universal primers (515F-806R) and sequenced with the Illumina MiniSeq platform (2263bp).