title: Illumina MiSeq paired-end Sequencing of KAS22011037 (KAE22126770)
design: Each of three to four groups of six 4-weeks old female mice (C57BL/6) group was orally admisitrated with each of 200 ul of V. vulnificus MO6-24/O wild type (CFU 10+e7) or luxS-deletion mutant (CFU 10+e22), every day for four weeks. At starting point and ending point of the experiment, stool samples from mice were collected, and microbiome was analyzed (Chun Lab)
library_construction_protocol: RNA was extracted from wild type V. vulnificus strain MO6-24/O grown in LB broth to an A600 of 1.0. The primer PE-leuO, which is complementary to the coding strand of leuO, was 32P-end labeled and used to synthesize cDNA from 1 microgram of total RNA using PrimeScript RT Enzyme mix I Takara Bio Inc., Japan. The same primer was used to generate a sequencing ladder using the Top DNA Sequencing Kit Bioneer, Daejeon, Korea. The resulting primer extension product and sequencing ladders were resolved on a 6% polyacrylamide sequencing gel. The gel was dried and read using a BAS-1500 Imaging Plate Fujifilm, Tokyo, Japan.