title: GSM8858627: SW1990 cell ，Vector-1; Homo sapiens; OTHER
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes.  After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). CUT&Tag libraries were prepared using the KAPA HTP Library Preparation Kit complemented withNEXTflex DNA Barcodes from Bio Scientific.10ng of DNA was used as starting material for input and ip samples.   Libraries were amplified using 13 cycles on the thermocycler.   Post amplification libraries were size selected at250-450bp in length using AgencourtAMPure XP beads from Beckman Coulter.   Libraries were validated using the Agilent High SensitivityDNA Kit.