title: 16S rRNA gene sequencing of the lake sediments
design: PCR amplification of 16S rRNA gene was performed using 27F and 1492R universal primers. Library preparation was performed using Oxford Nanopore Native Barcoding Kit 24 V14. Sequencing was preformed using pores of version R10.4.1. Basecalling was performed with Dorado v7.4.14 using SAP calling model.