title: GSM8486088: G751C_5L24_Ontogency; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from each sample using the Paxgene Blood RNA kit (catalog no.: 762164;Qiagen, Valencia, CA, USA) following the manufacturer's protocol. RNA quantification and quality assessment was performed using an Agilent 2100 Bioanalyzer (Santa Clara, CA, USA) The NEBNextPoly(A) mRNA Magnetic Isolation Module (catalog no.: E7490L, NEB; Ipswich, MA, USA) was used to capture poly-adenylated RNA. Strand-specific cDNA libraries were generated using the KAPA RNAHyperPrep Kit (catalog no.: 08098107702, Roche; Basel, Switzerland) on a Freedom EVO 100 (Tecan,Männedorf, Switzerland).