title: 16S rRNA of top-down SynComs
design: gDNA was extracted following protocols from Song et al., mSystems, then DNA was submitted to SeqCenter for sequencing. The samples were sequenced for 50k reads and every sample reached >80k reads with most samples achieving >100k reads. Samples were prepared using Zymo Researchs Quick-16S kit with phased primers targeting the V3/V4 regions of the 16S gene with the following sequences: CCTACGGGDGGCWGCAGCCTAYGGGGYGCWGCAG (forward) and GACTACHVGGGTATCTAATCC (reverse). Following clean up and normalization, samples were sequenced on a P1 600cyc NextSeq2000 Flowcell to generate 2x301bp paired end (PE) reads.