title: Anopheles coluzzii RNAseq after ENH07 silencing
design: Anopheles coluzzii RNAseq after ENH07 silencing
library_construction_protocol: Three-day old female females Anopheles coluzzii mosquitoes were injected with dsENH07 or dsGFP, and after 2 days, total RNAs from three biological replicates were extracted and sequenced. For each biological replicate, pools of ~10 mosquitoes were extracted per treatment group. Three-day-old cold-anaesthetized mosquito females were injected using the NanoJect II (Drummond) either with 1.5mg of an equal quantity of ds1, ds2 and ds3 (500 ng each per mosquito) or with 1.5mg of dsGFP for the control group as described above. Two days post-injection, for each condition, total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.). The use of 3dsRNA was to optimize the targeting and silencing of the ENH07 eRNA. Three independent experiments were performed and the extracted total RNA from each condition and each experiment were submitted to transcriptome analysis.  total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.) The libraries were prepared with the illumina TruSeq Stranded mRNA kit