title: GSM9205793: Hepatopancreas, healthy, atlas1; Penaeus vannamei; RNA-Seq
library_construction_protocol: Hepatopancreas was removed from shrimp, gently chopped into pieces, and placed into 2 mL of dispase for 15 min at 37°C. After treatment, 10 mL of phosphate buffered saline (PBS) was added, and the tissue was gently passed through a 70 μm and then 40 μm cell sieve to create single-cell suspensions. 10x Genomics 3' GEM-X kit (v4) and Chromium X Instrument following manufacterer protocol