title: GSM8600354: WT_D7; Mus musculus; RNA-Seq
library_construction_protocol: Right lobes of the lungs were collected and cut into small pieces and digested with 180 U/ml Collagenase Type 2 (Worthington Biochemical) for 30 min at 37 °C, followed by disruption using gentleMACS tissue dissociator (Miltenyi). Single cell suspension was obtained after red blood cell lysis, filtration through 70 μm mesh, and washed with flow cytometry buffer. Single cell suspension was centrifuged and re-suspended in flow cytometry buffer For library preparation, we utilized the Chromium Single Cell 3′ Reagent Kit from 10x Genomics, strictly adhering to the manufacturer's guidelines