title: Control replicate 2
design: Yeast cultures of Saccharomyces cerevisiae CZ were treated with 0 mg/L octanoic acid in YPD medium (containing 1% ethanol as solvent control) for 6 hours at 30C with 180 rpm shaking. Total RNA was extracted using TRIzol reagent, followed by DNase I treatment and purification with Qiagen RNeasy columns. RNA integrity was verified via Agilent Bioanalyzer (RIN > 8.0).   Strand-specific RNA-seq libraries were prepared with the Illumina TruSeq Stranded mRNA Kit. Polyadenylated mRNA was enriched using oligo-dT beads, fragmented, and reverse-transcribed into cDNA. After adenylation of 3' ends, Illumina adapters were ligated, and libraries were amplified with 12 PCR cycles.   Paired-end sequencing (2150 bp) was performed on the Illumina NovaSeq 6000 platform, generating ~10 Gb raw data per sample. Three biological replicates were included for each treatment group.