title: ChIP-seq of M. smegmatis MKD8 deletion of whiB6 with 20 minute induction of 3xHA-N-term-WhiB6 in TSBT broth
design: Cells were cultured in TSBT broth to OD600 ~ 0.8. ATc was added to 100 ng/mL and culturing was continued for 20 minutes. Cultures were crosslinked with 1% formaldehyde and quenched with excess glycine. Cells were pulverized in a Retsch cryomill and DNA sheared by 30 min in a Bioruptor sonicator. DNA-protein complexes were collected on EZview Red Anti-HA Affinity Gel. Immunoprecipitated DNA was purified with the QiaQuick PCR purification kit. Libraries were prepared from the enriched DNA fragments using the NextUltra II Library Prep kit (NEB). Over 10M paired-end reads were mapped to the reference genome for MACS2 analysis.