title: Untreated control
library_construction_protocol: Yeast genomic DNA was extracted from the haploid Saccharomyces cerevisiae strain CEN.PK113-7D by using the MasterPure Yeast DNA Purification Kit (MPY80200, LGC). High molecular weight yeast genomic DNA was uniformly amplified by using the REPLI-G mini whole genome amplification kit (150023, Qiagen). In brief, 20 ng of yeast genomic DNA was used as a template and amplified by incubating with REPLI-G DNA Polymerase at 30 Â°C for 16 hours. Amplified yeast genomic DNA was purified by using 1.8x Ampure XP beads (10136224, Beckman Coulter) and eluted in 10 mM Tris-HCl, pH 8.0, or double-distilled water. Amplified yeast genomic DNA was fragmented and size-selected for 10-15 Kb by using g-tube (520079, Covaris).