title: GSM9376614: RNA-seq, leaf, Koshihikari, green manure, Year 2023, rep4; Oryza sativa; RNA-Seq
library_construction_protocol: The largest leaf of vegetative 'Koshihikari' plants sampled at 47 DAT (2023) and 38 DAT (2024) was collected. Each leaf (one biological replicate) was collected between 09:00 and 10:00, immediately frozen in liquid nitrogen and stocked until sample crushing. Frozen samples were crushed by a multi-specimen cell disruption device (BMS-A20TP; BMS) just before RNA extraction. Total RNA from each sample was extracted using an RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's protocol. Three to four biological replicates were obtained for each cultivation condition. RNA-sequencing (RNA-seq) libraries were prepared using the NEBNext Ultra II Directional mRNA-seq kit for Illumina (New England Biolabs), following the manufacturer's protocols.