title: GSM8714993: A53; Rattus norvegicus; RNA-Seq
library_construction_protocol: On Day 11 or 21, rats were sacked, kidneys harvested and glomeruli were isolated by sieve method. Total RNA was extracted. The mRNA libraries were generated using the following kits and conditions: NEBNext® Ultra™ II Directional (stranded) RNA Library Prep Kit for Illumina (NEB #E7760L) NEBNext Poly (A) mRNA Magnetic Isolation Module (NEB #E7490) NEBNext Multiplex Oligos for Illumina Unique Dual Index Primer Pairs (NEB #6442S/L) Library input amount: 200ng total RNA (quantified using Qubit Fluorometer) and total RNA achieved a RIN of >7 Fragmentation: 10 minutes PCR: 12x Libraries were sequenced with Illumina NovaSeq 6000 flow cell using paired-end 150-bp format to at least 17 million passed-filter clusters/sample (equivalent to 34 million reads)