title: Oilfield reservoir 16S rRNA metabarcoding
design: Sequencing libraries were prepared with indexed adapters, quantified using a Qubit fluorometer (Invitrogen, Thermo Fisher Scientific) and real-time PCR (Applied Biosystems), and assessed for fragment size distribution using an Agilent 2100 Bioanalyzer. Quantified libraries were pooled and sequenced on the Illumina MiSeq PE300 platform (Illumina, San Diego, CA, USA) based on the required sequencing depth