title: RNA-Seq of E. coli Nissle grown on gluc1, replicate 1
design: Total RNA was extracted from Escherichia coli strains K-12 MG1655 and Nissle 1917 grown in M9 minimal medium with either sulfoquinovose or glucose as the sole carbon source. RNA samples were treated with DNase I and subjected to rRNA depletion using the Illumina Ribo-Zero kit. Directional RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, and sequenced as 150 bp paired-end reads on the Illumina NextSeq 500 platform.