title: day 10 silk gland nuclear matrix associated DNA sequence_replicate 1
design: DNA libraries were prepared with Illumina-compatible NEXTflex Rapid DNA sequencing Bundle (BIOO Scientific, Inc. U.S.A.) at Genotypic Technology Pvt. Ltd., Bangalore, India. 50 ng of genomic DNA was sheared using Covaris S2 sonicator (Covaris, Woburn, Massachusetts, USA) . About 1 0 ng of fragmented DNA were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTflex Rapid DNA sequencing bundle kit protocol.Adapter-ligated DNA was purified using CamSelect NGS beads (Cambrian Bioworks, # CBWD010 ) . Resultant fragments were amplified for 8 cycles of PCR using Illumina-compatible primers provided in the NEXTflex Rapid DNA sequencing Bundle. Final PCR products (sequencing libraries) were purified with CamSelect NGS beads (Cambrian Bioworks, # CBWD010 ). Finally, the sequencing libraries were quantified by Qubit fluorometer (Thermo Fisher Scientific, MA, USA) and the fragment size distributions were analyzed on Agilent 4150 TapeStation