title: RNA-seq (3'BRB-seq), Col-0, low nitrogen (0.5 mM NH4NO3), Day 6 drought, leaf, replicate 3
design: Total RNA was extracted from Arabidopsis (Col-0 and nlp7)  leaves using the Qiagen RNeasy Plant Mini Kit, following the manufacturers instructions. Libraries were constructed using the Bulk RNA Barcoding and Sequencing (BRB-seq) protocol (Alpern et al., 2019), which introduces sample-specific barcodes during reverse transcription, allowing early multiplexing. After pooling and amplification, sequencing-ready libraries were generated following the BRB-seq workflow. Libraries were sequenced on an Illumina platform, and data are provided to SRA as demultiplexed FASTQ files per sample.