title: GSM8821980: Tfap2a_Control1; Mus musculus; RNA-Seq
library_construction_protocol: Mouse kidneys of 12-week-old male Hoxb7Cre+;Tfap2afl/fl or control mice (n = 2 per group) were dissected and washed in ice-cold PBS. A middle slice of 1–2 mm was taken, and the cortex was trimmed to enrich for medullary cell types. Mouse kidney specimens were stored in pre-cooled RNAlater (Invitrogen # AM7020) at 4°C for 24 hours and then stored at -80°C until nuclei isolation as reported in Leiz, Hinze et al (J Vis Exp, 2021). All samples were subjected to single-nuc sequencing following the 10x Genomics protocol (Document #CG000204 Rev D) for Chromium Next GEM Single Cell 3' v3.1 chemistry targeting 9,000–10,000 nuclei.