title: GSM8600518: SPY-S4 Hi-C; Sus scrofa; Hi-C
library_construction_protocol: 2.0×106 cells were fixed with 37% formaldehyde and quenched formaldehyde with glycine for 10min at RT. Cells were washed by PBS for three times and then centrifugal pellet of cells, snap-frozen, and stored at -80℃. Resuspended cell pellets in lysis buffer on ice for 50min and then the nuclei were digested with 200U MboI for 1h at 37℃. Filled restriction fragment overhangs and sheared DNAs to 300-500bp, biotinylated DNAs were pulled down with streptavidin beads. Hi-C：Submission of DNA reads to Illumina NovaSeq 6000 for sequencing, we generated data from 6 samples (two for SPG, two for SPY and two for RS), totaling approximately 2.7 billion valid interactions, with an average of 448 million valid interactions per sample.