title: GSM9529325: S021_Tumor_Pre_R1; Homo sapiens; RNA-Seq
library_construction_protocol: Microdissected material was collected in 0.5 ml Zeiss adhesive cap tubes (Carl Zeiss Microscopy, Jena, Germany; one tube per slide). Dissections were completed within 60 min, with dissected areas ranging from ~7 × 10⁴ to 1.4 × 10⁶ µm². RNA was extracted in 150 µl PKD buffer using the RNeasy FFPE Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. RNA integrity (RIN) and DV200 were assessed with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Whole transcriptome was captured from 1ng of tumoral RNA. Libraries were prepared using Takara Bio SMART-Seq mRNA LP reagents following the manufacturer's recommendations.