title: GSM9231981: xsmallrna_160; Drosophila virilis; miRNA-Seq
library_construction_protocol: Females of the 101 and 1051.47 strains were collected for DNA extraction and aged for 10-15 days. Genomic DNA was isolated from thirty pairs of ovaries using high-salt denaturing buffer containing 100 mM Tris-HCl (pH = 8), 10 mM EDTA (pH = 8), 350 mM NaCl, 2% SDS, and 7 M Urea following phenol-chloroform extraction. Before sequencing, we performed a size selection of high molecular weight DNA by depleting short DNA fragments below 10 kb using the Short Read Eliminator Kit (Circulomics, USA) The preparation of DNA libraries for sequencing was carried out using the 1D ligation SQK-LSK109 kit (Oxford Nanopore Technologies (ONT), UK), by the recommended protocol from ONT. Sequencing was performed on a flow cell FLO-MIN-106D r9.4 (ONT, UK). The result was an average of 9.5 billion bases sequenced, with a median length of sequenced fragments from 20 to 40 kb for different libraries.