title: GSM8756025: AT2 cells, knockout, H3K27me3 CUT&RUN, rep 2; Mus musculus; OTHER
library_construction_protocol: AT2 cell CUT&RUN-seq was performed as described in the associated paper. CUT&RUN was carried on isolated chromatin from sorted cells (~100,000 from pools of two mice per sample) isolated by flow cytometry based on AT2 cell specific Sftpc-CreER reporter recombination. The KAPA HyperPrep kit (Roche, Basel, Switzerland) was used for library preparation. Assessment of library quality and quantity was done with Qubit 2.0 DNA HS Assay (ThermoFisher, Massachusetts, USA), Tapestation High Sensitivity D1000 Assay (Agilent Technologies, California, USA).