title: GSM9295497: strong tumor bio rep3; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Toal RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) according to the manufacturer's protocol. RNA quality was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA,USA) and checked using Rnase free agarose gel electrophoresis. After total RNA was extracted, eukaryotic mRNA was enriched by Oligo(dT) beads. Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversly transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for lumina(NEB #7530,New England Biolabs, Ipswich, MA, USA).