title: Target-Capture of Cannabis sativa: leaf
design: DNA was fragmented using ultrasound bath sonicator for 30 min, DNA fragments were END-repaird and cleaned using MagSi-NGSPREP Plus, samples with magnetic beads were separated and beads were washed. Adapters were ligated and NGS libraries were amplified and cleaned using MagSI-NGSPREP beads. Libraries were quantified using qPCR and pooled into four reactions. Hybridization Capture-based Target Enrichment was performed. Four captured reactions were poold into one single reaction. For sequencing, the Ion Torrent PITM Controle Ion SphereTM Particles were used. Sequencing was performed on an Ion ProtonTM Sequencer using the Ion PITM v3 Chip.