title: BSA-seq of the Clubroot Resistance Locus in Brassica Oleracea
design: To randomly shear DNA into fragments of approximately 350 bp using ultrasonic methods, the DNA fragments then undergo end repair, 3' end addition of an 'A', ligation with sequencing adapters, purification, and PCR amplification to complete the construction of the sequencing library. After the library passes quality control, sequencing is performed using the Illumina HiSeq platform.