title: GSM9635845: 29hpf-ef1a-mCherry_01; Danio rerio; RNA-Seq
library_construction_protocol: 29 hpf dechorionated embryos were enzymatically dissociated into a single cell suspension. Samples were washed twice before resuspending in 400 µL of Resuspension Buffer (10% OptiPrep (Sigma, D1556), 1x PBS, and 0.1% BSA in ddH2O) for single-cell microfluidic barcoding. An ONYX microfluidics platform (Droplet Genomics, DG-ONYX), V2 barcoding PDMS chips (Droplet Genomics DG-CBC2-80-10), and inDrops V3 hydrogel beads were used to encapsulate single cells for cDNA barcoding. Droplet Genomics protocol was followed for library preparation.