title: 6 months KO
design: RNA sequencing libraries were prepared using mRNA isolated from total RNA via poly-T oligo-attached magnetic beads. The mRNA was fragmented and converted to cDNA using random hexamer primers. Libraries were prepared using an unstranded workflow, including steps of end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Sequencing was performed on the NovaSeq X Plus platform (PE150).