title: ATAC-seq of Homo sapiens: Adult CD8
design: Approximately 500 thousand cells were frozen in RecoveryTM Cell Culture Freezing Medium (Thermofisher #12648010) and stored at -80C. An aliquot of 50 thousand cells was then used for ATAC-seq library preparation following the Omni-ATAC protocol as released by the Kaersten lab (Corces et al. 2017; Amanda Ackermann 2019).  medRxiv preprint doi: https://doi.org/10.1101/2023.07.19.23292550; this version posted July 23, 2023. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license . Library size was checked by TapeStation 4200 and QC was done by quantstudio using the NEBNext Library Quant Kit for Illumina. Sequencing was performed on the NextSeq2000 platform at a target depth of 50 million reads per library and generating 50bp paired-end reads.