title: scRNA-seq of Homo sapiens - vaccine skin site
design: All skin samples were utilized immediately (i.e. fresh) following     dissociation. Dead cells were immunomagnetically depleted using the Dead Cell     Removal Kit (Miltenyi), washed, and resuspended in PBS with 0.04% bovine serum     albumin at a concentration of 1,000 cells per µL. Approximately 17,000 cells were     loaded onto a 10X Genomics Chromium instrument (10x Genomics). Libraries were     prepared using the Chromium single-cell 5' library and gel bead kit. Following     library construction, quality control, and quantification, libraries were pooled and     sequenced