title: 16.29 (CRX733397; CRA011697)
library_construction_protocol: Total DNA was extracted using the ALFA-SEQ Advanced Soil DNA Kit. The V4 hypervariable region was amplified of the prokaryotic 16S rRNA gene of bacteria and archaea, and 12 bp barcodes were added to the 30-ends of the primers to distinguish different samples. Then equal molar amounts of DNA were pooled for library construction.