title: GSM9253311: MDA-MB-231 cells, dasatinib treatment, 12h_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from cultured cells using TRIzol reagent (Thermo Fisher Scientific, 15596026) and then purified using the RNeasy mini kit (QIAGEN, 74104). Complementary DNA (cDNA) libraries for RNA-seq were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs, E7770) and subjected to quality control. Sequencing was conducted on the NovaSeq 6000 platform (Illumina) using 150-base pair paired-end reads.