title: GSM8821987: Enteric Neurons under CR infection in Colon, 10x single-nucleus RNAseq, Gene Expression; Mus musculus; OTHER
library_construction_protocol: Flow-sorted GFP+ nuclei were processed through the GemCode Single Cell Platform using the GemCode Gel Bead, Chip and 3'-end Library Kits by 10X Genomics,following the manufacturer's protocol Flow-sorted ILCs and Th cells were processed through the GemCode Single Cell Platform using the GemCode Gel Bead, Chip and 3'-end Library Kits by 10X Genomics,following the manufacturer's protocol The nuclei/ cells were partitioned into the GemCode instrument.The products were subjected to reverse transcription, emulsion breaking, cDNA amplification, and sample index attachment (10×Genomics). Libraries were pair-end (150+ 150 bp)