title: GJT_D1_Nano1 (CRX742066; CRA011861)
library_construction_protocol: A total amount of 2 microg DNA per sample was used as input material for the ONT library preparations. After the sample was qualified, size-select of long DNA fragments were performed using the BluePippin system (Sage Science, USA). Next, the ends of DNA fragments were repaired and A-ligation reaction were conducted with NEBNext Ultra II End Repair/dA-tailing Kit (Cat# E7546). The adapter in the LSK109 kit was used for further ligation reaction and Qubit 3.0 Fluorometer (Invitrogen, USA) was used to quantify the size of Library fragments. submitted_instrument_model: OXFORD_NANOPORE PromethION