title: Soil-Lake Inundation Moat Experiment_Water Column
design: DNA was extracted with a CTAB method followed by purification by phenol-chloroform-isoamyl alcohol.  The 18S rRNA gene was amplified from samples using the Earth Microbiome primers 1391f and EukBr with Illumina adaptors.  Samples were cleaned with Sequal Prep and indexed with Nextera XT library prep kit.