title: GSM8651528: CD8, SGC, 8days, biol rep3; Mus musculus; RNA-Seq
library_construction_protocol: 1x10e6 were suspended in RLT supplemented with 2-mercapthoethanol and snap frozen. Total RNA was isolated with the RNAeasy mini kit (Qiagen). 2 ug total RNA (in RLT buffer) isolated from S. cerevisae was added as a reference. Samples with an RNA integrity number >6 were subjected to library preparation cDNA libraries were generated using the TruSeq Stranded mRNA sample preparation kit (Illumina)