title: WGS of Homo sapiens: PTA-amplified single DLD-1 cell 2 from clone H (Illumina sequencing)
design: This study utilizes an in vitro single-cell evolutionary model of DLD-1 (ATCC CCL-221) cells, systematically expanded and cloned over three filial generations (~90 days). Single cells from the parental population and selected clones were isolated via flow sorting and subjected to whole genome amplification using Primary Template-directed Amplification (PTA; BioSkryb ResolveDNA v1). Sequencing libraries were prepared using the Kapa HyperPlus workflow and sequenced using an Illumina NovaSeq6000 at the New York Genome Center (NYGC).