title: GSM9253450: dna_cdca7acdca7b_rep2; Arabidopsis thaliana; Bisulfite-Seq
library_construction_protocol: 14-day old seedlings were harvested from Col-0, cdca7a, cdca7b, cdca7a cdca7b+/-, cdca7a cdca7b, h1.1 h1.2, h1.1 h1.2 cdca7a cdca7b, and ddm1-2. Samples were immediately frozen in liquid nitrogen. DNA were extracted using the DNeasy Plant Mini kit (Qiagen). RNA was extracted using the Direct-zol RNA MiniPrep kit (Zymo Research). A total of 100ng DNA from was sheared to ~200bp using the Covaris S2 (Covaris). Then the libraries were constructed using the Ovation Ultralow Methyl-seq kit (NuGEN), and bisulfite conversion was achieved using the Epitect Bisulfite Conversion kit (QIAGEN). Finally, the libraries were sequenced on Illumina Novaseq X plus instruments.