title: PacBio HiFi sequencing of Gambusia sexradiata sulfidic ecotype: adult, male, skeletal muscle
design: Tissues were homogenized using an Omni Bead Ruptor 96 using a 2.8-mm ceramic bead per sample or by cryofracture using liquid nitrogen and a Covarias tissueTUBE. A 2-hour proteinase K digestion was included to increase yield due to small amounts of initial tissue input. DNA concentration was assessed using the Qubit High Sensitivity DNA kit in triplicate, Quantus Fluorometer, and Nanodrop. DNA quality and fragment size were evaluated using an Agilent Femto Pulse system and TapeStation. SMRTbell library preparation and sequencing was performed at the UC Davis Genome Center using two SMRT cells on a PacBio Revio sequencer.