title: GSM8756954: 1h/LB-rep2, SVb3-3; Vibrio cholerae C6706; RNA-Seq
library_construction_protocol: Bacterial cultures were then pelleted, and total RNA was isolated using Lysozyme and RNeasy (Qiagen) per manufacturer's instructions. RNA was depleted of ribosomal RNA using a ribosomal depletion kit (NEB) and RNA-seq libraries were constructed using NEBNext Ultra II Directional kit per manufacturer's instructions.