title: GSM8821280: colon_infected_d5_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Transcriptome sequencing (bulk RNA-seq) was performed on the colons of BALB/c on different days after Salmonella Typhimurium gavage (Days 0,1,3,5 and 7, n=3). The colons were washed 5 times with PBS, preserved in RNALater (Beyotime) at -80℃ until further use. The RNA extraction, RNA library preparation and sequencing were carried out by Novogene. sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, USA), with index-coded samples clustered according to the manufacturer's instructions using a cBot Cluster Generation System and the TruSeq PE Cluster Kit v3-cBot-HS (Illumina). Libraries were sequenced using an Illumina NovaSeq 6000 to produce 150 bp paired-end reads.