title: GSM8791418: D51-Scrambled; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using RNeasy Mini Kit (Qiagene, #74104) with DNA digestion (Qiagene, #79254) according to manufacturers instructions. RNA pola-A enrichment, preparation and sequencing was performed by Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Sequencing was perforimed using Illumina NovaSeq platform (HWI-ST1276).