title: RNA-seq of mus musculus: adult male aorta
design: Total aorta RNA was isolated, and RNA sequencing and data analysis were performed by Novogene Bioinformatics (Beijing, China) as previously reported [34]. Differentially expressed genes (DEGs) were assessed with the following threshold: adjusted P value <0.05 and |log2 Fold Change|>0.585. Gene set enrichment analysis (GSEA) was performed via GSEA v4.1.0. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) sets were independently used for GSEA. The enrichment scores (ESs) were calculated based on weighted KolmogorovSmirnov-like statistics, and the magnitude can indicate the relationship between a gene set and the phenotype. The higher the ES of the gene set, the higher the possibility that it was considered enriched in a particular group