title: Nanopore sequencing of Cas9 amplified from adenovirus in MACE: Campaign 1 Passage 1
design: Adenoviral DNA was isolated from 200 microliters of virus-containing media using the QuickDNA/RNA Viral Kit (Zymo Research). The dCas9 open reading frame was then PCR-amplified using Q5 HighFidelity DNA Polymerase (NEB) and dCas9-specific primers.