title: 16S rRNA gene sequencing of gut microbiota from T2DM patient (female, age 53) without hearing impairment
design: Fecal DNA extracted using PowerSoil DNA Isolation Kit. V3-V4 hypervariable region of 16S rRNA gene amplified using primers 341F/806R. Paired-end sequencing (2x250 bp) on Illumina MiSeq platform.