title: Cell culture cDNA Seq. of Saccharomyces cerevisiae: new1d_KanMX,ski2d_Leu2+P+IVPA-2
design: culutred in CSM media at 20 C and 220 rpm to log phase at OD600nm 0.5-0.9. IVPA=in-vitro poly-adenylation. P=PNK treatment for 3'-healing