title: WGS of Homo sapiens: PTA-amplified single DLD-1 cell 1 from clone E (UG sequencing)
design: This study utilizes an in vitro single-cell evolutionary model of DLD-1 (ATCC CCL-221) cells, systematically expanded and cloned over three filial generations (~90 days). Single cells from the parental population and selected clones were isolated via flow sorting and subjected to whole genome amplification using Primary Template-directed Amplification (PTA; BioSkryb ResolveDNA v1). Sequencing libraries were prepared using the Kapa HyperPlus workflow, converted for compatibility with the Ultima Genomics (UG) platform, and sequenced at the New York Genome Center (NYGC) to an average depth of 30 per cell.