title: GSM9205681: Ste12_WT_none_2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Following bead beating and sonication, chromatin from TAP-tagged yeast strains was incubated overnight on IgG-conjugated magnetic Sepharose beads. Libraries were prepared from immunoprecipitated DNA as described in the ChIP-exo 5.1 protocol (Rossi et al. 2018) pooled at equimolar concentration, and size selected by agarose gel excision (200-500bp). Sequencing was performed in-house on an Element Biosciences AVITI sequencer (2 x 75bp high output kit) to an average of 10M paired-end reads per sample, with data management, quality control, and processing through the PEGR automated workflow (Shao et al. 2022).