title: GSM8757265: Liver CD45+ cells enriched with F4/80+ cells, WT, PBS, ADT; Mus musculus; OTHER
library_construction_protocol: Liver tissue was mechanically fragmented and enzymatically digested with Collagenase D and DNAse I. Liver leukocytes were isolated via density centrifugation with 37.5 % Percoll. Cells were stained with fluoresence-activated cell sorting (FACS) antibodies and CITE-seq antibodies. Sorted CD45+ liver cells were enriched with CD3-CD19-CD45+F4/80+ cells. Cellular suspensions were loaded on a Chromium Single Cell 5′ GEM, Library & Gel Bead Kit (5' (v2) Dual index, 10X Genomics) according to the manufacturer's instructions.