title: GSM9674456: SNI3; Mus musculus; RNA-Seq
library_construction_protocol: At day-28 post-surgery, nuclei were extracted using the detergent-based chemical lysis method from PMC8964935 using 0.5% Triton X-100, RNase inhibitors, and DTT to disrupt cell membranes and preserve RNA in the nuclei. These nuclei were stained with NeuN and Hoecsht before FANS. Following FANS, nuclei were counted and  assessed using trypan blue inclusion.. Approximately 25,000 nuclei for each condition Sham, SNI and SNI+Transplant (3 biological replicates for Sham/SNI, 2 for Tranplant; all males) were captured in the 10X Genomics Chromium Controller using the Single Cell 3' Gene Expression kit (v3.1). Gel bead-in-emulsion (GEM) generation, reverse transcription, and cDNA amplification were performed according to the manufacturer's protocol. Libraries were constructed with their respective library preparation kits and quantified by Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform to achieve a minimum depth of ~60,000 reads per cell.