title: Element sequencing of RNASeq library of adult male abdomen pool Quadrastichus erythrinae USDA-ARS-TPGMBRU Wild
design: Total RNA was extracted from a pool of four male abdomens through homogenization of snap frozen tissue in Tri Reagents and then extraction using the Zymo Direct-zol Magbead Total RNA kit (Zymo Research, Irvine, CA) on a Kingfisher Flex 96 system (ThermoFisher, Waltham, MA). From total RNA, a poly(A) cDNA library was generated using the NEB Ultra II RNA Library Prep Kit (New England Biolabs, Ipswich, MA) using oligo(dT) selection. The resulting library was sequenced on one 2 x 150 Cloudbreak FS Sequencing Kit on an Element Biosciences Aviti system.