title: Complete genome sequencing of Pseudomonas sp. W52
design: Genomic DNA extraction was performed according to TaKaRa MiniBEST Universal Genomic DNA Extraction Kit protocol. The concentration and quality of extracted DNA was determined using NanoDrop2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA), Qubit dsDNA BR assay kit (Thermo Fisher Scientific, Wilmington, DE, USA) and agarose gel electrophoresis. Purified genomic DNA was transfered into a PromethION flow cell and sequenced on Oxford Nanopore PromethION 24 platform (P24) according to manufacturer instructions.