title: Illumina HiSeq 2000 paired-end Sequencing of KAS22011577 (KAE22553310)
design: RNA-seq was performed using the ADPKD mouse model (Pkd1, Pkd39 conditional KO) to identify miRNAs involved in cyst formation and related regulatory mechanisms.
library_construction_protocol: In order to construct cDNA libraries with the TruSeq RNA library kit, 1ug of total RNA was used. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 100nt paired-end sequencing by Illumina HiSeq2000. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2103 Bioanalyzer. The transcript counts in isoform level and gene level were calculated, and the relative transcript abundances were measured in FPKM Fragments Per Kilobase of exon per Million fragments mapped using Cufflinks.