title: 16S rRNA gene sequencing of gut samples from C57BL/6J
design: DNA was extracted from fecal or gastrointestinal samples using the ZymoBIOMICS Quick-DNA Fecal/Soil Microbe 96 Magbead Kit. We targeted the V3V4 region of the 16S rRNA with the 341F and 805R primers. Library preparation was carried out with dual-indexing using Nextera XT Index Kit Set A and Set D (Illumina)