title: GSM9377043: Spatial transcriptomics of whole bone marrow from KrasG12D/+ mouse; Mus musculus; OTHER
library_construction_protocol: Frozen bone marrow sections (10 μm) were cut using a cryostat, placed on BMKMANU S1000 spatial transcriptomics slides, fixed in 3.7–3.8% formaldehyde, stained with H&E, and imaged using bright-field microscopy. Pre-permeabilization was performed with collagenase and BSA in HBSS, followed by pepsin-mediated permeabilization to release RNA for library preparation. Spatial transcriptomic libraries were generated following BMKMANU S1000 protocols, including reverse transcription, second-strand synthesis, adapter ligation, indexing PCR, and purification. Libraries were sequenced on Illumina NovaSeq 6000 with 150-bp paired-end reads, targeting ≥50,000 reads per spot.