title: Single-Cell RNA Sequencing of Brain Endothelial Cells in 12-Month-Old Male 5XFAD and Wild-Type Mice
design: Single-Cell RNA Sequencing of Brain Endothelial Cells in 12-Month-Old Male 5XFAD and Wild-Type Mice
library_construction_protocol: Brains from male hemizygous 5XFAD (n=3) and wild-type control mice (n=3) aged 12 months were used. Brain tissue (excluding olfactory bulbs and cerebellum) was dissociated into a single cell suspension, and Fluorescence Activated Cell Sorting (FACS) was used to isolate CD31+, CD45- cells Following Fluorescence Activated Cell Sorting (FACS), total RNA was extracted from the isolated CD31+, CD45- brain endothelial cells. Nucleic acid isolation was performed according to the manufacturer's protocol. Single-cell gene expression libraries were generated using the 10x Genomics Chromium Next GEM Single Cell 5'Gene Expression, v2 Chemistry kit. Libraries were prepared according to the manufacturer's protocol, and raw data were later processed using Cell Ranger against the GRCm39-2024-A reference