title: GSM8545696: PN5; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) accordingtothemanufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed usingtheAgilent2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The libraries were constructedusingVAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions. Thetranscriptome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China)