title: GSM9527706: Visium_alpha_MHC_MerCreMer_Het; Mus musculus; OTHER
library_construction_protocol: Mouse hearts were harvested, fixed in formalin, and embedded in paraffin. FFPE blocks were sectioned at 5 μm thickness and mounted on microscope slides. Sections were deparaffinized, H&E stained, imaged, and processed using the 10x Genomics Visium CytAssist Spatial Gene Expression FFPE workflow, including decrosslinking, probe hybridization, and RNA capture according to manufacturer's instructions. Spatial transcriptomic libraries were generated using the 10x Genomics Visium CytAssist Spatial Gene Expression for FFPE protocol. Following probe hybridization and extension, libraries were constructed with paired-end dual indexing. Libraries were quantified, quality controlled, and sequenced on an Illumina NovaSeq platform (28 cycles Read 1, 10 cycles i7, 10 cycles i5, 50 cycles Read 2).