title: RNA-seq of mus musculus:C57_ACE_2
design: The RNA-seq library construction process was performed as follows: Total RNA was processed using mRNA enrichment, where mRNA with polyA tails was enriched using magnetic beads coated with Oligo(dT). The enriched RNA was fragmented using a fragmentation buffer. Random N6 primers were used for reverse transcription to synthesize the first cDNA strand, followed by second-strand synthesis to generate double-stranded DNA. The double-stranded DNA underwent end repair (blunting and 5' phosphorylation) and was A-tailed at the 3' end to create cohesive termini. A Y-shaped adapter with a single "T" overhang at the 3' end was ligated to the A-tailed DNA. The ligation products were amplified via PCR using adapter-specific primers. The PCR products were heat-denatured into single-stranded DNA and circularized using a bridge primer to form single-stranded circular DNA libraries. Finally, the constructed libraries underwent quality control (QC), and qualified libraries were subjected to sequencing.