title: GSM8544680: Ezh2b, wild type, IgG; Mus musculus; OTHER
library_construction_protocol: Utilizing the Hyperactive® Universal CUT&Tag Assay Kit for Illumina (TD903), we performed CUT&Tag experiments as follows: MEFs were harvested at room temperature and incubated with ConA Beads for 10 minutes. They were then incubated with anti-EZH2 (CST, 5246),  anti-H3K27me3 (CST, #9733) antibodies overnight at 4°C. On the next day, the samples were incubated with a secondary antibody at room temperature for 1h, followed by incubation with pA/G-Tnp for 1h.  After DNA purification with the MinElute kit, and the PCR was performed to amplify the library for 12 cycles and libraries were purified with the MinElute kit. Amplified  libraries were sequenced on the Illumina Novaseq 6000 platform. Amplified ChIP-seq libraries were sequenced on the Illumina Novaseq 6000 platform.