title: 16S V4 of Surfside Parking Structure
design: AccuPrime High Fidelity kit and 515F/806R primers containing single-end barcodes targeting the variable region 4 of the 16S rRNA gene were used. The PCR products were pooled and commercially sequenced using Illumina MiSeq paired end 2  250 bp flow cell and the MiSeq Reagent Kit V3 (600 Cycles; llumina).Cutadapt v2.10 was used to remove adapters from sequencing reads and to truncate reads using the quality trimming algorithm based on a desired Q-score of 30. After truncation, reads of less than 50 base pairs in length were discarded. Quality-controlled reads were then used to generate ASVs via dada2 v1.16.0. Paired FASTQ reads were trimmed and filtered to remove reads containing Ns or >2 sequencing errors per read. Forward and reverse reads were merged by overlapping sequences, and chimeras were removed.