title: day 23 ECNLD-REV Washout "OFF
design: DNA libraries were prepared using the Nextera XT DNA Library Preparation kit (Illumina) and IDT Unique Dual Indexes with total DNA input of 1 ng. Genomic DNA was fragmented using a proportional amount  fragmentation enzyme. Unique dual indexes were added to each sample followed by 12 cycles of PCR to construct libraries. DNA libraries were purified using AMpure magnetic beads (Beckman Coulter) and eluted in QIAGEN EB buffer. DNA libraries were quantified using Qubit 4 fluorometer and Qubit dsDNA HS Assay kit. Libraries were then sequenced on Illumina HiSeq X platform 2x150 bp.