title: RNA-seq for subject 260-1101, primary tumor
design: RNA-Seq libraries were prepared using a transcriptome capture approach. 850 ng of RNA was used for first strand cDNA synthesis using Illumina's TruSeq RNA Exome kit. Synthesis of the second strand of cDNA was followed by indexed adapter ligation. Subsequent PCR amplification enriched for adapted fragments. The amplified libraries were quantified using an automated PicoGreen assay.