title: Chloroplast Genome Sequencing and Comparative Genomics Analysis of Three Hypericum Species
design: 1. With 1  Establishing a DNA initial quantity library;2. Covaris M220 ultrasound breaks DNA to 300-500bp;3. Leveling, adding A at the 3 'end, and connecting the index connector (TruSeq)  Nano DNA Sample Prep Kit;4. Library enrichment, PCR amplification of 8 cycles;5. 2% agarose gel recovery target band (Certified Low Range Ultra Agarose);6. Quantitative analysis of TBS380 (Picogrreen), mixed on machine according to data ratio;7. Perform bridge PCR amplification on the cBot solid-phase carrier to generate clusters;8. Illumina NovaSeq sequencing platform for 2 * 150bp sequencing.