title: GSM9564844: Batch 1 of samples, multiplexed by DNA-barcoded antibodies - GEX; Mus musculus; RNA-Seq
library_construction_protocol: Mice splenocytes were isolated and subjected to cell straining to obtain a splenocyte suspension, stained for CD45, CD3, CD19, CD11b using fluorophore-conjugated antibodies and live/dead dye. Mouse T cells (CD45+ CD3+ CD19- CD11b- dead dye-) were sorted using flow cytometric sorting. The cells were then stained with DNA-barcoded hashtag antibodies (Biolegend TotalSeq-B) according to the manufacturer's instructions and pooled into 2 sets of 10 barcoded samples. The 2 pooled cell suspensions were subjected to 3' single-cell transcriptomic library preparation using the 10X Genomics kit according to the manufacturer's instructions (Single Cell 3' v3). The resulting libraries comprise gene expression (GEX) and antibody-dependent tag (ADT) libraries.