title: B15
design: DNA samples were sheared into ~400 bp fragments using a Covaris M220 Focused Acoustic Shearer following  manufactures protocol. Illumina sequencing libraries were prepared from the sheared fragments using  the NEXTFLEX Rapid DNA-Seq Kit. 5primer ends were first end-repaired and phosphorylated. Next ,  the 3 ends were A-tailed and ligated to sequencing adapters. The third step is to enrich the  adapters-ligated products using PCR. The prepared libraries then were used for paired-end Illumina  sequencing (2  150 bp) on Illumina Novaseq 6000 (or other new sequenator) (Illumina Inc., San Diego, CA, USA)