title: GSM9495128: S6k2-RK 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from samples using column-based RNA isolation methods following the manufacturer's instructions. RNA quantity and integrity were assessed prior to library preparation. Poly(A)+ mRNA was enriched from total RNA and strand-specific sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina according to the manufacturer's protocol. Libraries were adaptor-ligated, PCR-amplified, and sequenced as paired-end 150 bp reads on an Illumina platform, generating approximately 40 million paired-end reads per sample.