title: GSM9202683: Gcn4_WTAA SM_induced_with Rapamycin_Replicate 2; Saccharomyces cerevisiae; ChIP-Seq
library_construction_protocol: Sonicated chromatin extracts were immunoprecipitated with anti-TBP (from Joseph Reese's lab), anti-Rpb1 (Biolegend, Cat#664906), anti-Gcn4 (in-house),and anti-TFIIB (from Stephen Buratowski's lab). Immunoprecipitated chromatin were treated with proteinase K and DNA were extracted with chloroform and precipitated with iso-propanol. Libraries were prepared using DNA Library Prep Kit for llumina (Cat# E7370) from New England Biolabs.