title: GSM8505980: HEK293T-DGCR8-KO (1A5) small RNA library replicate 1; Homo sapiens; miRNA-Seq
library_construction_protocol: The total RNAs were isolated from the cells by using TRIzol Reagent (Invitrogen, 15596018) following the manufacturer's mannual. The small RNA fraction was separated from extracted total RNA using 12% denaturing urea-PAGE and was purified using the IPA precipitation method. Small RNA libraries were then prepared using NEBNext® Small RNA Library Prep Set for Illumina (E7330S) with the purified small RNA fraction as input. The final dsDNA libraries were subjected to a next-generation sequencing platform in a paired-end sequencing mode.