title: GSM8681189: MC38_IgG_m04_bulk; Mus musculus; RNA-Seq
library_construction_protocol: Two sections measuring 100 microns of each fresh frozen tumor block were collected in microcentrifuge tubes. The tubes were kept on dry ice and shipped to Azenta Life Sciences (South Plainfield, New Jersey, USA) for downstream RNA sequencing. Total RNA was extracted from fresh frozen tissue samples using Qiagen RNeasy Plus Universal mini kit (Qiagen cat# 73404), followed by poly(A) enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs cat# E7490). Strand-specific RNA sequencing library was prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs cat# E7760S) following manufacturer's instructions.