title: GSM9294393: Whole Human saphenous vein, Day 0, patient #1; Homo sapiens; OTHER
library_construction_protocol: Formalin-fixed, paraffin-embedded (FFPE) human saphenous vein tissues were sectioned at 5 µm thickness using a microtome. Sections were mounted onto 10x Genomics Visium Spatial Gene Expression FFPE slides, baked, deparaffinized, and H&E-stained according to the manufacturer's Visium FFPE Tissue Preparation Guide. Slide brightfield images were acquired prior to probe hybridization and library construction. Spatial transcriptomics libraries were prepared using the 10x Genomics Visium Spatial Gene Expression FFPE kit according to the manufacturer's instructions. Following probe hybridization, ligation, reverse transcription, and cDNA amplification, libraries were indexed and pooled. The pooled libraries were sequenced as 28×91 bp paired-end reads on an Illumina NovaSeq system following the Visium FFPE recommended workflow.