title: GSM9379332: Full Length RNase E replicate 2; Mycolicibacterium smegmatis; RNA-Seq
library_construction_protocol: RNA was extracted as described in (Vargas-Blanco DA, Zhou Y, Zamalloa LG, Antonelli T, Shell SS 2019. mRNA Degradation Rates Are Coupled to Metabolic Status in Mycobacterium smegmatis. mBio 10:10.1128/mbio.00957-19.) rRNA was depleted as described in (Culviner, P.H., Guegler, C.K. and Laub, M.T. (2020) A simple, cost-effective, and robust method for rRNA depletion in RNA-sequencing studies. MBio, 11, 10.1128/mbio. 00010-00020.). Libraries were constructed as described in Culviner, Peter H. et al. Cell, Volume 188, Issue 23, 6620 - 6635.e14.