title: Illumina NovaSeq 6000 paired-end Sequencing of NC (KAE23759922)
design: lentiviruses with TTR-targeted shRNAmiR were produced through lipofectamin2000, transducted into cells, and total RNAs were extracted via Trizol. Then RNAseq library prep was performed via NEBNext Ultra II RNA Library Prep Kit for Illumina
library_construction_protocol: lentiviruses with TTR-targeted shRNAmiR were produced through lipofectamin2000, transducted into cells, and total RNAs were extracted via Trizol. Then RNAseq library prep was performed via NEBNext Ultra II RNA Library Prep Kit for Illumina