title: GSM9253545: mRNA, Subjects UNC0218 and UNC0219; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood mononuclear cell preparations were enriched for B cells from the same patient and sequentially labeled with sample tags using the BD Human Single-Cell Multiplexing Kit and BD AbSeq oligo-conjugated antibodies. Single cells were captured, and cDNA was synthesized on beads, followed by targeted amplification of transcripts using the Human Immune Response Panel primers (399 genes) and a custom supplemental panel set (20 targets), as well as amplification of cDNA of sample Tag and AbSeq. Final RNA-seq libraries were prepared by performing the index PCR using a combination of 3 forward index primers (TreSeq D501-D503) and 12 reverse index primers (TrueSeq D701-D712).