title: Poultry waste treatment
design: DNA was extracted using a Nucleospin soil DNA extraction kit (Macherey-Nagel, Dren, Germany) following the manufacturers instructions. The internal transcribed spacer (ITS1 and ITS2) regions of the fungal ribosomal RNA gene were amplified using the universal primer pairs ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS2 (GCTGCGTTCTTCATCGATGC). The amplicons were gel purified, end-repaired, and Illumina-specific adaptor sequences were ligated. After quantification, the samples were individually indexed and underwent an additional purification step. Sequencing was carried out on Illuminas NovaSeq 6000 platform using a paired-end 2  150 bp configuration, generating approximately 20 Mb of data per sample.