title: GSM8681822: HUDEP-2 Guide 4 Rep1; Homo sapiens; RNA-Seq
library_construction_protocol: HUDEP2 cells were harvested and washed in 1× PBS before lysis and RNA was isolated using TRI™ reagent (Sigma-Aldrich) according to the manufacturer's instructions. RNA was purified using the RNeasy® Mini Kit and RNase-free DNase set (Qiagen) according to the manufacturer's instructions. For CD34 cells, RNA samples were extracted with QIAGEN RNeasy micro Plus kit. Stranded mRNA prep (Illumina) sequencing libraries were generated and RNA sequencing was performed by the Ramaciotti Centre for Genomics using NextSeq 1×75bp high output or NovaSeq 2x100bp flow cells (Illumina). For CD34 cells, RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.