title: Novel substrate affinity of FaCCR1 and FaCCR1 / FaOCT4 expression control the content of medium -chain esters in strawberry fruit
design: Novel substrate affinity of FaCCR1 and FaCCR1 / FaOCT4 expression control the content of medium -chain esters in strawberry fruit
library_construction_protocol: Fruits from contrasting lines with high or low MCE content. Each bulked pool was generated with equivalent amount of ripe fruit from 10 F1 lines from the '232' x '1392' mapping population selected based on high or low concentration of six selected medium-chain esters Standard commercial strawberry cultivation in the Huelva region (Spain) involves growing plants under plastic tunnel structures to optimize microclimate conditions. The soil is prepared through disinfection and then covered with black polyethylene mulch, which reduces weed growth, conserves soil moisture, and regulates soil temperature for improved plant establishment and yield. Total RNA was extracted from 300 mg fruit tissue following the CTAB protocol described in Gambino et al., 2008. The integrity of the RNA samples was assessed by agarose gel electrophoresis and further verified using a 2100 Bioanalyzer (Agilent, Folsom, CA), which resulted in RIN values ranging between 8.0 and 8.9 for the six samples. RNA was break in suitably small pieces by enzymatic treatment. After fragmentation, partial Illumina adapters were ligated to the RNA fragments' ends, which facilitates subsequent reverse transcription, amplification, and sequencing. Fragmentation and adapter ligation together ensure that RNA fragments are compatible with Illumina sequencing and retain strand information for antisense transcript detection.