title: DNA-Seq of Oncorhynchus Kisutch (OKe)
design: DNA was extracted muscle tissue of salmon using Qiagen DNeasy Blood and Tissue Kit. Tissues of the samples were lysed in the presence of Proteinase K and lysis buffer. Then, the purified DNA samples were amplified using the mitochondrial COI gene by PCR reaction. The samples that presented the expected amplicons of DNA (650bp) had been sent to FIRST BASE for purification and sequencing using Sanger Sequencing. The obtained sequences were visualised, aligned and edited using Chromas and BioEdit.