title: Illumina HiSeq 2000 sinlge-end Sequencing of KAS22011586 (KAE22553760)
design: MiRNA-seq was performed using the ADPKD mouse model (Pkd1, Pkd13 conditional KO) to identify miRNAs involved in cyst formation and related regulatory mechanisms.
library_construction_protocol: Small RNA sample preparation was done according to Illumina's protocol. In brief, 5'and 3' adapters were sequentially ligated to small RNA of 18-30 bases gel purified from 5-10 micro total RNA. Adapter-ligated small RNA was reverse transcribed, amplified and sequenced on a HiSeq2000 Illumina following manufacturer's instructions. Raw data the reads for each miRNA were normalized to the total reads of each individual sample as the standardized to reads per RPM; miRNA counts / total count of each sample x 1 million.