title: Pig lung microbiome
design: A total of 30ng of qualified genomic DNA was used as input for PCR amplification using either 16S rRNA gene or ITS region fusion primers. Amplified products were purified using Agencourt AMPure XP beads, eluted in Elution Buffer, and subsequently labelled for library construction. The quality and concentration of the libraries were assessed using an Agilent 2100 Bioanalyzer. Libraries that met the quality thresholds were sequenced on the DNBSEQ platform by BGI.