title: Nanopore PCR-cDNA sequencing of drosophila melanogaster: adult head
design: Total RNAs were in vitro polyadenylated using E. coli Poly(A) Polymerase (NEB, catalog #: M0276S). RNA libraries were prepared from 500 ng of in vitro polyadenylated RNAs using the PCR-cDNA barcoding kit from Oxford Nanopore (ONT, catalog #: SQK-PCB114.24) as per the manufacturer's instructions.