title: GSM9378494: Left ventricular, MI+PBS; Mus musculus; RNA-Seq
library_construction_protocol: heart tissue was homogenized and lysed with Triton X‐100 in RNase‐free water for nucleus isolation. Isolated nuclei were purified, centrifuged, and resuspended in PBS with RNase inhibitor, then diluted to 700 nuclei/μL for standardized 10x Genomics capture library preparation protocol with 10x Genomics Chromium Next GEM 3′ Single Cell Reagent kits version 3.1 (10x Genomics, Pleasanton, CA)