title: GSM9495980: Spry4 G-KO  spermatogonia, BU+PD-D10, rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from fresh spermatogonia isolated by FACS using PicoPure RNA isolation kit (KIT0204, Applied Biosystems). Residual genomic DNA was removed by on-column DNA digestion (79254, Qiagen). The stranded mRNA sequence libraries were generated using the KAPA mRNA HyperPrep Kit (Kapa Biosystems, KK8580).