title: GSM9494010: DynaMITE-control; Homo sapiens; RNA-Seq
library_construction_protocol: Dynamic single-cell RNAseq (DynaMITE-seq) : Component cells of the MITE devices were extracted from the devices for single-cell RNA sequencing following enzymatic digestion of collagen-hydrogel gel. First, MITE devices were incubated for 30 min with a solution of digestive enzymes comprising 100 U/mL collagenase type IV (Life Technologies) and enzymes from the human tumor dissociation kit (Miltenyi Biotec). Devices were then washed with 0.25% trypsin-EDTA (Thermo Fisher Scientific) for 5 mins and cells were collected by washing the device twice with Vasculife with 10% FBS. Single cell suspensions were loaded onto a 10X Chromium instrument (10X Genomics) per the manufacturer's instructions. Single-cell RNA libraries were generated using the Chromium Single Cell 5' Library Construction (10X Genomics) per user guide. Quality control of the completed libraries was performed using the Bioanalyzer High Sensitivity DNA Kit (Agilent) and then sequenced using the Illumina NextSeq 500 platform.