title: RNA-seq of mus musculus:adult male spleen N84
design: Total RNA was extracted based on the manufacturers protocol by using TRIzol reagent (Invitrogen). RNA libraries were prepared using NEBNextUltra II Directional RNA Library Prep Kit for Illumina (NEB) based on the manufacturers instructions. Illumina HiSeq X Ten platform (150-bp paired-end reads) was adopted by OEBiotech Co. Ltd. to sequence the libraries.