title: RNA_Seq_D9C_SG
design: RNA-seq libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit, involving mRNA isolation, fragmentation, cDNA synthesis, adapter ligation, PCR amplification, and quality validation using Qubit Fluorometer and Agilent 4150 TapeStation, followed by downstream analysis including trimming, alignment (STAR), read counting, and functional annotation/pathway analysis.