title: GSM9300824: Cisplatin R2; Homo sapiens; RIP-Seq
library_construction_protocol: The cells were collected, washed twice in ice-cold PBS, and pelleted at 300 × g for 5 min at 4 °C. Pellets were resuspended in 500 µL lysis buffer provided with RNA immunoprecipitation kit (MBL Life Science, RN1005) , vortexed, incubated for 10 min at 4 °C, and centrifuged at 12,000 × g for 5 min. . The lysates were incubated overnight at 4 °C with15 µg of BG4 antibody (Anti-DNA/RNA BG4, scFv fragment, FLAG-tagged, Absolute antibody, Ab00174-30.126). Llysates were incubated with washed M2 beads for 2 hours at 4 °C. The beads were then washed three times with 1 mL of wash buffer. Bead-bound RNA was extracted using the TRIzol method. The rRNA-depleted stranded libraries were prepared with the KAPA RNA HyperPrep Kit with HMR RiboErase (Roche, KR1351 v4.21). Library quality was assessed using the TapeStation D1000 High Sensitivity assay. The libraries were sequenced at a depth of 40 million reads.