title: GSM8824279: MFe15; Mus musculus; RNA-Seq
library_construction_protocol: We placed dissected tissue in ice cold DMEM w/o Phenol Red (Hyclone #SH30284.02). We dissociated tissues using the gentleMACS Octo Dissociator using the soft tumor program 37C_m_TDK1. We placed the cell suspension in a MACS SmartStrainer (70 µM) placed in a 15 mL conical tube, then washed it in 10 mL of DMEM. We then centrifuged the sample at 300 G for 7 min. at 4°C, removed the supernatant, and resuspended cells in 100 µL of PBS w/ 0.04% BSA. We processed cell suspensions with a 10X Single Cell 3' v3.1 kit, aiming to isolate ~10,000 cells Libraries were prepared according to the 10X Genomics protocol