title: GSM9637140: Wildtype B-cell progenitors; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow from a C57BL/6J and an IκB – mouse was isolated and lymphoid progenitors were magnetically enriched as previously described using the biotinylated antibodies at a concentration of 1:50 in staining panel 12. Following enrichment of the lymphoid progenitors, the cells were surface marker-stained using staining panel 9 as previously described. The cells were then sorted with the BD FACSAriaIII sorter in UCLA Janis V. Flow Cytometry Core Laboratory with adequate compensation setup by using UltraComp eBeads™ Compensation Beads and live cells with staining antibodies prior to actual sample sorting. We sorted approximately 204,000 WT and IκB – fraction A-B cells and added 10ng of yeast RNA (strain BY7473, generous gift from T.L. Johnson lab) per 100,000 cells as an internal control. The RNA samples were then submitted to UCLA Technology Center for Genomics & Bioinformatics for library preparation and were sequenced using Novaseq X Plus 10B (1250M/lane).