title: GSM8600662: p20185-s002_20-17_16_138_D0; Macaca mulatta; RNA-Seq
library_construction_protocol: RNA was isolated from blood samples stored in PAXgene tubes at the Yerkes National Primate Center for library preparation (www.yerkes.emory.edu/nhp_genomics_core/). NA quality was assessed using an Agilent 4200 TapeStation and concentration using the Qubit RNA HS assay (Thermo Fisher Scientific). Globin transcripts in the blood RNA were blocked with the FastSelect Globin Reagent (QIAGEN) before library preparation. Libraries were prepared using the Clontech SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) in combination with the NexteraXT DNA Library Preparation Kit to append dual-indexed adapter sequences (Illumina). Libraries were validated by capillary electrophoresis on an Agilent 4200 TapeStation, pooled at equimolar concentrations, and sequenced on an Illumina NovaSeq 6000 at 100SR, yielding 25 to 30 million reads per sample. Raw reads were examined for quality issues using FastQC (www.bioinformatics.babraham.ac.uk/projects/fastqc/) to ensure that library generation and sequencing are suitable for further analysis.