title: tNGS of patient number 99
design: The cDNA synthesis, multiplex PCR pre-amplification of target loci and library preparation were performed using the RP100 Respiratory Pathogen Microorganisms Multiplex Testing Kit (KingCreate, Guangzhou, China). Generated libraries were quantified using Equalbit DNA HS Assay Kit (Vazyme, Nanjing, Jiangsu, China) with Invitrogen Qubit 4.0 Fluorometer (Thermo Fisher, Waltham, MA, USA) to ensure all samples with library density  0.5 ng/L or else subjected for library reconstruction.