title: RNASeq of human immune cells
design: RNA isolation from purified cell populations (monocytes, neutrophils, or eosinophils) stored in RNAlater was performed using TRIzol (Invitrogen, USA), followed by RNA cleanup with RNeasy Mini Kit columns (Qiagen, USA). Isopropanol/water (1:1) was used for RNA precipitation. RNA integrity was assessed using an Agilent 2100 bioanalyzer. Poly(A)-enriched cDNA libraries were generated using the TruSeq v2 sample preparation kit (Illumina, CA) and checked for quality and quantity using bioanalyzer and quantitative PCR.