title: Transcriptome sequencing of Hishimonus sellatus
design: Total RNA was extracted from Hishimonus sellatus samples using Trizol reagent following the manufacturer's instructions. RNA integrity and quantity were verified using an Agilent 2100 Bioanalyzer. Poly(A) mRNA was enriched using oligo(dT) magnetic beads. The enriched mRNA was fragmented into short pieces. First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase, followed by second-strand cDNA synthesis using DNA Polymerase I and RNase H. The cDNA fragments were end-repaired, A-tailed, and ligated with sequencing adapters. After PCR amplification, the library was sequenced on an Illumina NovaSeq 6000 platform (PE150) to support gene prediction and annotation.