title: scRNA-seq of Mus musculus tamoxifen injected AIDCreERT2xR26eYFP mice
design: An AIDCreERT2xR26eYFP mouse was i.p. injected with tamoxifen at day 0. 3 weeks later immune cells from the small intestine were isolated and IgA+ cells were enriched via magnetic beads. IgA+ cells were further processed using the Chromium Next GEM Single Cell 5' Reagent Kit v2 according to the manufacturer's instructions (10x Genomics, Inc., United States). The experiment aimed for 5000 recovered cells. Sequencing was performed on an Illumina NextSeq 500.