title: GSM8599649: CORTEX_VEH_INPUT_LANE2; Rattus norvegicus; ChIP-Seq
library_construction_protocol: Nuclei isolation from rat midbrain and cortical tissue was performed following a previously published protocol with slight modifications DOI: 10.1038/s41596-020-00472-3 For library preparation, the NEBNext Ultra II DNA Library Prep kit (New England BioLabs, E7645) was used for End Prep and Adapter Ligation, following the manufacturer's instructions. Unique barcoded adapters (NextFlex, Bioo Scientific) were added. To decrosslink, libraries were incubated with RNase A (Thermo Scientific, EN0531) and Proteinase K (New England BioLabs, P8107) at 55°C for 1 hour and 65°C overnight. Libraries were then PCR amplified for 14 cycles with NEBNext Ultra II Q5® Master Mix (New England BioLabs, M0544).