title: GSM9055324: N153SLSL/LSL  mouse3; Mus musculus; RNA-Seq
library_construction_protocol: Cerebellar tissues were homogenized and total RNA was isolated with TRI Reagent™  (MilliporeSigma). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.