title: Lyophilized Levain - ITS
design: DNA samples were subjected to amplification of the entire 16S rRNA gene using primers 27F and 1492R (Lane, 1991) (~1.6 kb fragment) and of the ITS1ITS2 region using primers ITS5-1737F and ITS4-2409R (White et al., 1990) (650950 bp fragment). Amplicons were visualized on agarose gel and quantified. The resulting fragments were used to construct sequencing libraries and sequenced on the MinION platform (Oxford Nanopore) using Flongle Flow Cell (FLO-FLG001).