title: CH_EGSFR2218 (GEX)
design: Approximately 16,000-25,000 nuclei per sample were loaded per channel of the Chromium Next GEM Chip J for processing on the 10x Chromium Controller (10x Genomics, Pleasanton, CA, USA) followed by transposition or cDNA generation and library construction according to manufacturer's instructions (Chromium Next GEM Single Cell Multime ATAC + Gene Expression User Guide, Rev F). Libraries were normalized and pooled for sequencing on two NovaSeq SP-100 flow cells (Illumina, Inc., San Diego, CA, USA).