title: diabetic foot ulcer skin swab DNA
design: Genomic DNA was quantified using a Qubit fluorometer and assessed for integrity using an Agilent TapeStation. DNA was fragmented to an average insert size of approximately 300350 bp using acoustic shearing. Fragmented DNA underwent end-repair and A-tailing followed by ligation of Illumina-compatible adapters containing unique dual indexes. Adapter-ligated fragments were purified and size-selected using AMPure XP beads to remove small fragments and adapter dimers. Where required, libraries were enriched by limited-cycle PCR amplification. Final libraries were assessed for fragment size distribution on a TapeStation and quantified by qPCR. Libraries were normalized, pooled equimolarly, denatured, and diluted according to Illumina guidelines before sequencing on an Illumina HiSeq 4000 platform using paired-end chemistry