title: PacBio HiFi sequencing of Hishimonus sellatus
design: High molecular weight (HMW) genomic DNA was extracted from pooled whole bodies of Hishimonus sellatus using a standard genomic DNA extraction kit suitable for long-read sequencing. The quality and integrity of the DNA were assessed using pulsed-field gel electrophoresis and a Qubit fluorometer. For PacBio HiFi library preparation, the gDNA was sheared to an average size of 15-20 kb. The sheared DNA underwent DNA damage repair, end-repair, and ligation of hairpin adapters to form SMRTbell templates. The SMRTbell library was size-selected to remove short fragments. Sequencing was performed on the PacBio Sequel II platform in Circular Consensus Sequencing (CCS) mode to generate high-fidelity (HiFi) long reads for de novo genome assembly.