title: RNA-Seq of Charonia tritonis: Adult mantle tissue
design: Total RNA was extracted from tissue samples. RNA concentration and purity were measured using a Nanodrop 2000 spectrophotometer, with the requirements that RNA concentration must be 300 ng/L and the OD260/280 ratio must fall between 1.8 and 2.2. RNA integrity was assessed through agarose gel electrophoresis to detect RNA degradation or genomic DNA contamination. The RNA integrity number (RIN), 28S to 18S rRNA ratio, electrophoretogram baseline elevation, and 5S rRNA peak were precisely evaluated using an Agilent 2100 Bioanalyzer. High-quality RNA-seq reads were mapped to the C. tritonis reference genome using HISAT2 v2.0.8