title: RNA-seq of intestinal biopsy, UC non-inflamed
design: Total RNA was extracted using the Nucleospin RNA Plus kit (Macherey-Nagel) according to the manufacturers instructions. The purity of the RNA was assessed through spectrophotometric analysis with the NanoDrop ND-1000 (Thermo Fisher Scientific). Concentration and RNA quality were evaluated with the Qubit Fluorometer (Qubit Broad Range RNA kit, Thermo Fisher Scientific) and 2100 Bioanalyzer (RNA 6000 Nano kit, Agilent Technologies). The library was poly-A enriched for bulk RNA sequencing on the Illumina NovaSeq 6000 (v1.5 reagent kit) with 150bp unstranded paired-end reads.