title: GSM8545358: TvEV3; Homo sapiens; RNA-Seq
library_construction_protocol: Direct-zolTM RNA Miniprep Kit (Zymo Research; Cat. #R2071) according to the manufacturer's instructions Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.