title: Fungal ITS 2 amplicon sequencing of Soil metagenome ITS1
design: The fungal community composition was analyzed by targeting the ITS2 region using PCR primer pair ITS 3 (5'TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGCATCGTGAAGAACGCAGC3') as forward primer and ITS 4 (5'GTCTCGTGGGCTCGGAGATGTGATAAGAGACAGTCCTCCGCTTATTGATATGC3') reverse primer of r RNA gene from soil sample. Amplicon libraries were prepared using PCR and sequenced on illumina Mi sequence platform with paired end reads. The data were generated for diversity analysis.