title: GSM8598093: MM468_HACtrl276_d0_n2_HAFOSL1; Homo sapiens; OTHER
library_construction_protocol: The CUT&Tag-IT™ Assay Kit (Active Motif, Ref: 53160) was used with 1:50 Anti-Rabbit antibodies for FOSL1 (Cell Signaling, Ref: #5281), HA (Epicypher, Ref: 13-2010) and H3K27ac (Cell Signaling, Ref: #8173) following manufacturer instructions. Starting cell number was 500 000 cells for control and GOF cells down to 100 000 for persister cells. Digitonin concentration (0,05%) was maintained in all buffers, completing the kit with additional digitonin (Cell Signaling, Ref: #16359). Sequencing libraries were prepared using Nextera™- Compatible Multiplex Primers (Active Motif, Ref: 53155). CUT&Tag libraries were sequenced on a NovaSeq 6000 (Illumina) in PE100 mode.