title: GSM9605355: WT-HeLa, HSV-1 infection 20h,rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells with RAP80 knockdown were infected with HSV-1, and total RNA was extracted at indicated time points using a standard kit. After quality validation, mRNA was enriched via oligo(dT) beads, and strand-specific RNA-seq libraries were constructed. Libraries were quantified, pooled, and sequenced on a NovaSeq 6000 (Illumina) platform according to the manufacturer's instructions, generating paired-end 150 bp reads. Total RNA was extracted and DNase-treated. Poly(A) mRNA was isolated, fragmented, and used to construct a stranded RNA-seq library via first- and second-strand cDNA synthesis, adapter ligation, and PCR amplification. Libraries were sequenced on an Illumina platform (paired-end 150 bp) following standard Illumina protocols.