title: mpox3-V1.0 (CRX754523; CRA012029)
library_construction_protocol: a 10 microL volume of extracted DNA was subjected to whole genome amplification using the ATOPlex Multiplex PCR Amplification kit (MGI, China). PCR product was purified using MGI Clean Beads (MGI, China) and then prepared as a library using the MGIEasy Fast PCR-Free FS DNA Library Prep Kit (MGI, Shenzhen, China), according the manufacturer's protocol. The libraries were quantified using a Qubit 2.0 instrument (Invitrogen, USA) and normalized. Subsequently, the normalized libraries were circularized using the DNBSEQ OneStep DNB Make Reagent Kit (MGI, China) and converted into DNA nanoballs (DNBs). Finally, the DNB libraries were sequenced on the MGISEQ-2000 platform with SE100+10+12 according to the manufacturer's standard protocol. submitted_instrument_model: BGISEQ-2000