title: CLIA somatic whole exome sequencing of human melanoma (primary tumor)
design: Library construction was performed as described in Fisher et al. (PMID 21205303) with select modifications, including use of adapter ligation with IDT palindromic forked adapters with unique dual-indexed molecular barcode sequences. Kapa HyperPrep reagents in 96-reaction kit format were used for end repair and A-tailing, adapter ligation, and library enrichment PCR. Libraries within a library construction plate were pooled prior to hybridization. Hybridization and capture were then performed using the relevant components of Twist Bioscience's Broad Custom Exome Bait following the manufacturer's suggested protocol with select exceptions. All hybridization and capture steps were automated on the Agilent Bravo liquid handling system.