title: GSM8653162: 09_NCIH510_sgBRD4_1_Hypoxia_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted using the RNeasy mini kit from Qiagen (Qiagen, #74106) according to the procedure. mRNAs were enriched using the NEBNext poly(A) mRNA magnetic isolation module (NEB, #E7490L). Libraries were prepared using the NEBNext Ultra II Directional RNA library preparation kit (NEB, #E7760L) and sequenced on the NovaSeq 6000 (29).