title: Pooled multi-species plant RNA samples for PacBio Iso-Seq transcriptome sequencing
design: RNA was extracted from plant tissues using the Spectrum Plant Total RNA Kit (Sigma-Aldrich) or the RNeasy Plant Mini Kit (Qiagen). RNA integrity was assessed using an Agilent TapeStation instrument. Libraries were prepared following bulk Iso-Seq SMRTbell Prep Kit 3.0 (Pacific Biosciences). Sequencing was performed on PacBio Sequel II platform using SMRT Cell 8M and the Sequel II Sequencing Kit, according to the manufacturer's recommendations.