title: single nuclei RNAseq of Daphnia magna: old, non-reproducing, rep2
design: Using the NIM nuclei isolation protocol, we isolated nuclei from each sample of combined individuals with nuclei counted by the standard Cellaca AO/PI cell counting method and purified by FACS method. Libraries were prepared using Chromium Single Cell 5' v2 protocol and cleaned up using double-sided SPRI cleanup protocol.