title: GSM8681203: CX18A5; Mus musculus; RNA-Seq
library_construction_protocol: Samples were treated with RNALater (Ambion) and preserved at -80º until the RNA extraction procedure. Total RNA was isolated with the RNeasy Kit (Qiagen) following the manufacturer's instructions. The integrity of RNA was assessed with an RNA 6000 Nano chip with a Bioanalyzer platform (Agilent), and samples with RNA integrity number (RIN) ≥7 were included. RNA samples were quantified using Qubit (Thermo) and 1000 ng were used for constructing cDNA libraries with TruSeq Stranded mRNA Library Preparation Kit (Illumina) following the manufacturer's protocol. Libraries were quantified in Quibit and sequenced in the Illumina HiSeq 2500 platform to produce 2×100 bp reads.