title: NextSeq 500 paired-end Sequencing of KAS22011525 (KAE22133390)
design: missing
library_construction_protocol: We extracted Total RNA from the sample with Trizol following the manufacture instruction. Using TruSeq Stranded mRNA Library Prep Kit Illumina, San Diego, CA, we made library construction. Shortly, the experiment proceeded as follows: 1 Strand cDNA synthesis 2 Strand synthesized using dUTPs instead of dTTPs 3 End repair, A-tailing and adaptor ligation 4 PCR amplification. 5 Each library was diluted to 8 pM for 76 cycles of paired-read sequencing 2 X 75bp on the Illumina NextSeq 514 per the manufacturer's recommended protocol.