title: WGS homo sapien: homogenized stool
design: WMS library prep was carried out using Covaris focused-ultrasonication for fragmentation; Samples were fragmented using the following settings: Intensity: 10%; Duty cycle: 10%; Cycle/burst (CPB): 100; Time: 1 min. Fragmented DNA was analyzed using the Tapestation and average peak size was approximately 280 bp. Fragmented DNA was indexed using the New England Biolab (NEB) NEBNext Ultra II DNA Library Prep Kit for Illumina with NEBNext Multiplex oligos for Illumina (NEB cat # E7645S/L; E6440) following the instruction manual (V6.1_5/20) with 50 L of fragmented DNA as input; this corresponded to approximately 200  300 ng of DNA input