title: Oxford Nanopore sequencing of Vibrio cholerae: 5hmC-modified E1 strain genomic DNA
design: Based on the genome assembly from BioProject PRJNA888235 (RefSeq assembly: GCF_026013235.1, assembly name: ASM2601323v1). The cytosine in the library are fully substitute by 5hmC during PCR amplification. Library preparation was performed using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-LSK110) following the manufacturers protocol. The sequencing was carried out on a Nanopore MinION device. Reads are basecalled with reinforced 5hmC DeepSME basecaller at https://github.com/sparkcyf/DeepSME.