title: Comparative analysis of single-cell and spatial Nanopore long-read methods
design: Comparative analysis of single-cell and spatial Nanopore long-read methods
library_construction_protocol: Nanopore sequencing libraries were prepared with the Single Cell sequencing on Promethion protocol (Nanopore) with the SQK-PCS11 kit. We re-amplified 10 ng of the 10x Genomics PCR product for 4 cycles with 5′-CAGCTTTCTGTTGGTGCTGATATTGCAAGCAGTGGTATCAACGCAGAG-3′ and 5′ Biotine-CAGACACTTGCCTGTCGCTCTATCTTCCTACACGACGCTCTTCCGATCT 3′. After 0,8x AmpureXP purification to remove excess biotinylated primers, biotinylated is bound to Dynabeads™ M-280 Streptavidin beads (Invitrogen) and amplified with the primers cPRM for 4 cycles.