title: RNA-Seq Data and Expression Matrices for Brain and Liver from Mice Mutation Accumulation and Control Lines
design: RNA-Seq Data and Expression Matrices for Brain and Liver from Mice Mutation Accumulation and Control Lines
library_construction_protocol: The mouse samples sequenced in this study were part of a large mutation accumulation (MA) experiment described in previous studies (see Publications).  All tissue and RNA extractions were made from male mice with similar ages, ranging from 7 to 11 weeks old. Tissue extractions were always carried out at similar times during the day (10am-12am Central European Time) at the facilities of the Max Planck Institute. The whole brain was used for RNA extraction, and liver samples always came from the same liver lobe. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification