title: Transcriptome capture RNA-Seq of human renal cell carcinoma (primary tumor)
design: Libraries were generated from total RNA using Illumina's TruSeq RNA     Access Library Prep Kit. Synthesis of the second strand of cDNA was followed by     indexed adapter ligation. Subsequent PCR amplification enriched for adapted     fragments. The resultant cDNA libraries were hybridized to capture probes targeting     the exome and blocking oligos to reduce off-target binding. Streptavidin magnetic     beads were used to capture the library-bound probes, followed by washing steps.     After enrichment the libraries were quantified and then pooled equimolarly. All     library construction steps were automated on a liquid handling     system.