title: ChIPseq
library_construction_protocol: Sorted cells were fixed with 1% PFA for 10 minutes at room temperature and then quenched with glycine. Fixed cells were then centrifuged at 600 g, 5 minutes, 4C and the supernatant was removed. Cell pellets were stored at -80C until use. Cell pellet was resuspended in 100 ul nuclear lysis buffer (50 mM Tris-HCl pH 7.5, 10 mM EDTA, 1% SDS), supplemented with 1x protease inhibitor cocktail (Roche, 11873580001) and incubated on ice for 10 minutes. Sonication was performed on the Picorupter platform (Diagenode) for 5 cycles of 30s ON/30s OFF. Sheared chromatin was centrifuged at 10,000 g, 10 minutes, 4C and the clarified supernatant was transferred to a new DNA LoBind Eppendorf tube (Eppendorf). Chromatin immunoprecipitation was performed using the iDeal ChIP-seq kit for Histones (Diagenode; C01010173) as per manufacturer's recommendations. The reverse-crosslinked reaction was column purified with ChIP DNA Clean & Concentrator (Zymo Research; D5205) and library preparation was performed with the KAPA HyperPrep kit (Roche; KK8504) according to manufacturer's instructions. Libraries were sequenced on the NovaSeq platform (Illumina).