title: Genomic sequencing of Prunus sibirica
design: High-molecular-weight genomic DNA was first extracted and subjected to quality control to ensure integrity and purity. The DNA was then repaired and end-prepped to generate blunt-ended fragments with 5 phosphorylated and 3 A-tailed ends. Subsequently, sequencing adapters provided by Oxford Nanopore Technologies were ligated to the prepared DNA fragments using a ligation-based protocol. These adapters contain motor proteins that facilitate the translocation of DNA molecules through the nanopores during sequencing. The final library was purified to remove excess adapters and small fragments and immediately loaded onto the flow cell for sequencing.