title: LRS of aquaculture pond water breeding crayfish
design: Total genomic DNA was extracted using the DNeasy PowerSoil/Water Kits. For second-generation sequencing, DNA library was constructed using VAHTS Universal Plus DNA Library Prep Kit for MGI V2 , and then sequenced on the MGI DNBSEQ-T7 (PE=150 bp). For Oxford Nanopore sequencing, the libraries were prepared using the SQKLSK110 ligation kit and using the standard protocol. The purified library was loaded onto primed R9.4 Spot-On Flow Cells and sequenced using a PromethION sequencer.