title: GSM9253090: Human, Islets, shControl, Replicate 1 (RRID: SAMN47834917); Homo sapiens; RNA-Seq
library_construction_protocol: approximately 100 pseudoislets per condition were collected and RNA was isolated according to the manufacturer's protocol (Zymo Research). cDNA was first synthesized using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Clontech Laboratories, Inc.). A dual indexed cDNA library was then prepared using Nextera XT DNA Library Prep Kit (Illumina, Inc.). Each library was quantified, and its quality accessed by Qubit and Agilent Bioanalyzer, and multiple libraries were pooled in equal molarity. The average size of the library insert was approximately 300-400 bp