title: Illumina NovaSeq6000 Sequencing of Caur126 Transcriptomic Paired-end Library
design: Illumina NovaSeq6000 2x151 sequencing of Caur126 transcriptomic paired-end dual index library
library_construction_protocol: Total RNA isolation was done by ZymoBiomic RNA Miniprep Kit (R2001), then followed by cleaning up with DNase treatment and Monarch RNA cleanup up kit (T2030L).  Ribosomal RNAs were removed by QIAseq FastSelect -rRNA Yeast kit (334315).  First and second strand cDNAs were synthesized with Illumina stranded total RNA prep kit (20040529) and cleaned up with AMPureXP beads (A63881). Dual-index libraries for Illumina sequencing were created from selective PCR amplification of anchor-ligated cDNA fragments. Libraries were assayed for quality and concentration by Agilent 2100 Bioanalyzer and DNA 1000 kit.