title: ATAC-seq on batch of embryonated haecon-5 eggs
design: For each egg batch, ~100 mg of pelleted eggs was used for nuclei isolation and subsequently two ATAC-seq libraries were constructed according to manufacturers instructions (ATAC-seq animal tissues kit, Hologic Diagenode, New Jersey, USA). The libraries were quantified using the Qubit dsDNA High Sensitivity assay (Invitrogen, Thermofisher, Scientifics, Waltham, USA), and the quality of the library size was assessed using the 4200 TapeStation System Genomic DNA ScreenTape Assay (Agilent Technologies, Waldbronn, Germany). Each library was sequenced on one lane of a NovaSeq X (10B, 300 cycles; Illumina, San Diego, CA, USA) platform, generating 150 bp paired end reads stored in FASTQ format.