title: GSM9054595: CD33+cells from pancreatic cancer tissue.; Homo sapiens; RNA-Seq
library_construction_protocol: Surgically resected pancreatic cancer tissues from patients were minced into small fragments using a razor blade inin RPMI-1640 medium containing 10% FBS. Each sample was filtered through a 100-μm cell strainer and treated with Tumor & Tissue Dissociation Reagent to generate a single-cell suspension. Human myeloid cells were identified as CD45+ and CD33+. To isolate human myeloid cells from the single-cell suspentions, the cells were stained with the CD45 and CD33 monoclonal antibodies and Fixable Viability Dye. Then, the human myeloid cells were isolated by cell sorting with FACSAria II. single-cell sequencing libraries were prepared according to the manufacture's instruaction (Chromium single cell protocol 3' Library & Gel Bead kit, 10x Genomics).