title: I1 (CRX915602; CRA014277)
library_construction_protocol: Transcriptome library construction involves the purification and fragmentation of poly(A) RNA, followed by reverse transcription to generate cDNA, synthesis of U-labeled second-stranded DNAs, and modification of blunt ends with an A-base for adapter ligation. The adapter-ligated fragments undergo size selection, followed PCR amplification to create the final cDNA library with an average insert size of 300+/-50 bp.