title: RNA-Seq of tissue : piglet male liver
design: RNA was harvested using RNAsimple Total RNA Kit (Tiangen Biotech Co., Ltd., Beijing, China).  1.3 ug of total RNA was used for the construction of sequencing libraries. The total of 1 g RNA in each sample was used as the input material for RNA sample preparation.According to the manufacturer's suggestion, a NEBNext Ultra RNA Library Prep Kit (Illumina, Nebraska, USA) was used to generate a sequencing library.