title: GSM8789968: rTreg, PBS#6, Foxp3AIDTIR1(F74G), Spl+LNs; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using an in-house kit. Sorted cells were lysed with a buffer containing guanidine thiocyanate, Triton X-100 and 𝛽-mercapthoethanol. RNA was captured using magnetic beads. Following a series of wash steps, the beads were incubated with a DNAse I-containing buffer (NEB, cat #M0303) for 15 minutes at 37°C to degrade genomic DNA. After washing, the RNA was eluted in 20uL nuclease-free water for 5 mins at 60°C from pre-dried beads. RNA quality and concentration were assessed using the Agilent DNF-471 RNA kit and Agilent Fragment Analyzer. SMARTer Stranded Total Pico Input RNA Kit v3 (Takara, cat. #634487) was used according to manufactor protocol.