title: GSM8601730: hsdMS, 43°C, #1; Pseudomonas aeruginosa; OTHER
library_construction_protocol: Following cell lysis, the Zymo Genomic DNA Clean and ConcentratorTM kit was used as instructed to extract DNA. For each DNA sample, 100 ng was fragmented using NEBNext® dsDNA Fragmentase (NEB, catalog no. M0348S) at 37 °C for 20 min and then denatured in 0.1 M NaOH at 37 °C for 5 min. The denatured DNA was neutralized by 10% HOAc, purified by the Oligo Clean & Concentrator kit (Zymo), and mixed with 0.001% (w:w) spike-in probes containing one 6mA site and one inosine site (GTG TCT GGT GTT CTG TCG TGT GCT ACT C/iN6Me-dA/T CCG ATC TCG CAT C/ideoxyI/T CAC AGT ATT CGT CGT ATG AGA CAC AAC TAC ATG CTT GTC CGC TCT TGT GTC GGC T).