title: Whole genome sequencing of Equus kiang: adult male
design: After DNA quality control using the Femto Pulse system (Agilent, USA), DNA was randomly sheared into 1520 kb fragments using the Megaruptor 3 system (Diagenode) and then purified. Subsequently, DNA repair, end A-tailing, adapter ligation, and nuclease treatment were performed sequentially using the SMRTbell prep kit 3.0. The library was further size-selected and purified using the PippinHT system (Sage Science, USA). Finally, sequencing was completed on the PacBio Revio platform with the Revio Enzyme Binding Kit (Pacific Biosciences, USA) to obtain high-quality genomic sequencing data.