title: GSM9301454: aPS-MyeloidCapture2; Mus musculus; RNA-Seq
library_construction_protocol: Spleen were digested in 2mg/ml Collagenase D for 30 min at 37C. Samples were passed throught a 70um strainer, cells were then washed and stained for CD11b+, CD3, NK1.1, CD19. CD11b+ CD3- NK1.1- CD19- myeloid cells were sorted for scRNAseq. Single cell RNAseq libraries were made using the Chromium single cell 5' Library and Gel Bead kit (10x Genomics). Sorted cells were captured into Gel Beads-in-emulsion (GEM). Samples were reverse transcribed, and cDNA was isolated and pooled. Barcoded cDNA was fragmented, end repaired, and A-tails were followed by PCR.