title: RNA sequencing of Notopterygium incisum seeds
design: The RNA of the total sample was isolated and purified using TRIzol (thermofisher, 15596018). The amount and purity of the total RNA were then controlled using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA) and the integrity of the RNA was tested using Bioanalyzer 2100 (Agilent, CA, USA). When the RNA purity and integrity were qualified, the library was constructed. After the library quality inspection was qualified, it was sequenced using Illumina NovaseqTM 6000, and the sequencing read length was double-end 2*150bp (PE150).