title: Whole genome sequencing of Equus kiang: adult male
design: To the purified DNA, 10 New England Biolabs (NEB) Buffer 3.1 and 150 U of DPN II enzyme were added for digestion. Then, 10 mM dTTP, 10 mM dCTP, 10 mM dGTP, 5 mM biotin-14-dATP, water, and 40 U of Klenow enzyme were added to fill in the sticky ends. After ligation was completed, cross-linking was reversed, and the DNA was purified using the QIAamp DNA Mini Kit and sheared to a length of 400 bp. Dynabeads MyOne Streptavidin C1 magnetic beads were used to enrich the ligated adapters. The NEBNext Ultra II DNA Library Prep Kit was employed to construct Hi-C libraries for Illumina sequencing, and the final libraries were sequenced on the MGI-2000 platform.