title: GSM8652354: DHA MDA-MB-231 (sh-LUC) rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed with the QIAzol Lysis Reagent (Qiagen, Germany). RNA isolation was performed using miRNeasy Micro Kit (Qiagen, Germany) according to the manufacturer's protocol. Nanodrop ND-1000 (Thermo Fisher Scientific, USA) was used to assess quantity and purity of the extracted RNA. Total RNA samples were also QC-checked using Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Libraries for mRNA sequencing were prepared from total RNA samples using MGIEasy RNA Library Prep Set (MGI Tech Co., China). Ribosomal RNA was depleted using the MGIEasy rRNA Depletion Kit V1.2 (MGI Tech Co., China), following the manufacturer's instructions.