title: White Preadipocytes Diff R1
design: Whole transcriptome sequencing (RNA-seq) was performed using the following approach and quality acceptance criteria. RNA was extracted as described above. RNA was quality tested on an Agilent TapeStation, and all samples passed a minimum RIN score of 6.5, OD 260/280 above 1.8, and a concentration above 100 ng/L. Sequencing libraries were prepared using Illumina's TruSeq mRNA Stranded (Illumina, San Diego, CA) and indexed using Illumina Nextera RNA-seq barcoding kit. RNA-seq libraries were then subjected to 2  100 paired-end sequencing on an Illumina NextSeq 2000 at ATCC's Sequencing & Bioinformatics Center.