title: GSM8485734: D4_GE, replicate 2, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: The uterine tissue was extracted and cut into 2–3 mm fragments, which were then subjected to digestion at 37 °C for 1 h using pancreatin and dispase. Subsequently, the epithelial layer was carefully separated from the middle of the tissue fragments under a stereomicroscope, followed by rinsing with 1× PBS. The remaining tissue fragments were subjected to Accumax solution digestion, and the resulting cell suspension was filtered through a nylon mesh to remove excess cell debris and muscle layer. After separating the epithelial layer and muscle layer, red fluorescent positive cells, namely glandular epithelial cells, were obtained through flow cytometry sorting. Single-cell suspensions were loaded to 10x Chromium to capture single cell according to the manufacturer's instructions of 10X Genomics Chromium Single-Cell 3' kit (V3). The following cDNA amplification and library construction steps were performed according to the standard protocol.