title: RNA-seq of M. squalidus: Adult Female Midgut 2
design: Libraries were prepared using the Illumina TruSeq Stranded RNA Core Kit. mRNA was isolated with magnetic beads and fragmented before reverse transcription into first-strand cDNA. The synthesis of double-strand cDNA was completed using DNA polymerase I and RNase H (Illumina). cDNA fragments were amplified, adenylated, ligated to adapters and enriched with PCR to create a final cDNA library. Amplified fragments length and integrity was verified through electrophoresis on 1.5% agarose gel, using DNA ladder 1kb (Thermo Fisher Scientific) as a reference parameter.