title: Prime editing efficiency experiment in MCF10A cells for Val765Ile, D14 measurement of replicate 1
design: Genomic DNA was extracted by resuspending cell pellets in QuickExtract buffer (Lucigen, #QE09050) at a final concentration of 1600 cells/L and incubating them 5 min at 65C, 5 min at 68C and 10 min at 98C. 9.5 L of genomic DNA (15,200 cells) was then used for deep sequencing.