title: singlecell_RNAseq of B cells peritoneal lavage_run2
design: B cells from Nmur1Cre-T2A-GFP Id2flox/flox mice and littermate controls were applied to the 10X Genomics workflow for cell capturing and scRNA gene expression (GEX) library preparation using the Chromium Single Cell 5 v2 Library & Gel Bead Kit (10x Genomics). Final GEX libraries were obtained after fragmentation, adapter ligation, and final Index PCR using the Single Index Kit TT Set A. Qubit HS DNA assay kit (Life Technologies) was used for library quantification and fragment sizes were determined using the Fragment Analyzer with the HS NGS Fragment Kit (16000bp) (Agilent). Sequencing was performed on a NextSeq2000 device (Illumina) using a NextSeq 1000/2000 P2 reagent (200 cycles) with the recommended sequencing conditions for 5 GEX libraries (read1: 26nt, read2: 90nt, index1: 10nt, index2: 10).