title: RNA-Seq of mus musculus: adult female colon
design: Total RNA was extracted using TRIzol and assessed by Agilent 5300 Bioanalyzer and NanoDrop ND-2000; libraries were prepared using Illumina stranded protocols for standard samples and SMART-Seq V4 for low-input samples, quantified with Qubit, and sequenced on a NovaSeq X Plus platform.