title: GSM9252693: UTD T cells, Cas9 WT mRNA, mock, 48hrs post EP, donor ND637; Homo sapiens; RNA-Seq
library_construction_protocol: Cultured T cells were preserved in RNAprotect Cell Reagent with storage at -80C until processing. Total RNA was extracted using the Qiagen RNeasy Plus Universal Mini Kit. Strand-specific RNA sequencing libraries were prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB)