title: patient WH12
design: A custom capture panel of probes with a size of 26.55 Mbp   was designed for Leishmania aethiopica genome, using the reference genome sequence of the L146 strain and synthesized by Agilent Technologies. Noteworthy, this library should work very well with related species like L. tropica. The library preparation was done following SureSelect XT HS Target Enrichment System for Illumina Multiplexed Sequencing Platforms protocol (Agilent Technologies, Santa Clara, USA). In brief, a quantity of 25-200 ng of input gDNA was fragmented using SureSelect Enzymatic Fragmentation kit (Agilent technologies, Santa Clara, USA). Subsequently, adaptor-ligated libraries were prepared, hybridized with the custom probes at a dilution 1:10, and captured with Dynabeads MyOne Streptavidin T1 magnetic beads (Thermo Fisher Scientific, Waltham, USA). After washing steps, the DNA captured by streptavidin beads was amplified by PCR, and purified with AMPure XP beads. The quantity and quality of the libraries were assessed by TapeStation using High Sensitivity D1000 ScreenTape (Agilent Technologies, Santa Clara, USA). Sequencing was done in Genomescan (Leiden, the Netherlands) using Illumina NovaSeq 6000 platform, 150 bp paired-end reads.