title: GSM8600361: RMS, replicate6, scRNA; Homo sapiens; RNA-Seq
library_construction_protocol: Freshly harvest tumor transplanted on NSG mice were dissociated using tumor dissociation kit (Miltenyi Biotec, cat log. 130-095-929) according to the manufacturer's instruction to get single cell suspensions. Single cells dissociated from harvest tumor were immediately processed for library preparation using 10X Genomics Chromium Chip A/B Single Cell kit and Single Cell GEM, Library & Gel Bead kit (cat. nos. 1000092/100075 and 1000073/1000074), according to manufacturer protocols.