title: GSM8485826: Liver, INT-767, Rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Liver tissue was harvested from the left lateral lobe and snap frozen in liquid nitrogen. Tissue sections (about 50 mg) were homogenized in lysis buffer containing protease inhibitors and used for RNA extraction using NucleoSpin Plus RNA columns (Macherey-Nagel). The quantity of the RNA was analyzed using a Nano Drop 2000 spectrophotometer (Thermo Scientific, United States) RNA-seq libraries were prepared with NeoPrep (Illumina Inc., San Diego, CA, US) using Illumina TruSeq stranded mRNA Library kit for NeoPrep (Illumina) and sequenced on the NextSeq 500 (Illumina) with NSQ 500 hi-Output KT v2 (75 CYS; Illumina).