title: Complete genome sequence Tenacibaculum maritimum
design: The complete genome of T. maritimum SP9.1 strain, belonging to the O4 serotype and isolated from diseased Atlantic salmon (Salmo salar) in Spain, has been sequenced. Stock cultures of this strain were preserved at 80C in cryovials containing Flexibacter maritimus medium (FMM; Condalab, Spain) supplemented with 8% glycerol (8). For sequencing, a single colony from an FMM agar plate, incubated at 28C for 24 h, was used to inoculate 5 mL of FMM broth, which was again incubated at 28C for 24 h. Genomic DNA was extracted using the MagAttract HMW DNA kit (Qiagen, Germany), following manufacturers protocol for Purification of High-Molecular-Weight Genomic DNA from Gram-Negative Bacteria. The DNA integrity was verified by electrophoresis and Nanodrop analysis, and its concentration was quantified using an Invitrogen Qubit 3.0 fluorometer (Thermo Fisher Scientific, USA). Sequencing was performed on a PacBio Sequel IIe platform (10 kb library), with library preparation and sequencing services provided by the Central Support Service for Experimental Research (SCSIE) at the University of Valencia (Valencia, Spain). The SMRTbell Express Template Preparation Kit v3.0 was used for library construction. The PacBio sequencing yielded 343,665 reads with a Q20 score. A de novo genome assembly was performed using Flye version 2.9-b1768 with a long-read only assembly approach (Table 1) (9).