title: cDNA-amplicon sequencing of bioreactor sample : bacterial community in a PNAD2 reactor (PNAD2-C)
design: Biomass sample used for NGS was collected from a PNAD2 reactor at 98 d. The biomass was used for the RNA extraction. The cDNA was immediately synthesized after the RNA extraction. The obtained cDNA was used as template for high throughput sequencing. A universal bacteria primer set (i.e., 341F and 805R) was used to construct the library.