title: GSM9563748: Monocyte-derived macrophages from Donor ND607b, Untrained, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: 100,000 cells were collected per replicate and lysed in buffer containing 10% NP-40, 10% Tween-20, and 1% digitonin for 3 minutes on ice, then transposed with Tn5 for 30 minutes at 37˚C. Transposed DNA was then isolated with a Zymo DNA Clean and Concentrator kit. Libraries were amplified by PCR with custom ATACseq primers, purified by double-sided AMPure XP magnetic bead selection, and quantified by BioAnalyzer High Sensitivity chips.