title: WGS of Mycobacterium abscessus subsp. abscessus: clinical lung sputum isolate
design: Genomic DNA was fragmented using a Covaris LE220 instrument and processed with the NEBNext Ultra II DNA Library Prep Kit for Illumina. DNA was end-repaired, 3 adenylated, and ligated to adapters, followed by limited-cycle PCR enrichment. Libraries were validated with the Agilent 5300 Fragment Analyzer and quantified using the Qubit 4.0 Fluorometer. Multiplexed libraries were sequenced on an Illumina NovaSeq 6000 using a 2150 bp paired-end (v1.5) configuration. Image analysis and base calling were performed using NovaSeq Control Software v1.7. Raw .bcl files were converted to FASTQ and demultiplexed with bcl2fastq v2.20, allowing one mismatch for index recognition.