title: GRBV Transcriptomics Symptomatic Replicate 1
design: A transcriptomic analysis was performed on RNA from six symptomatic and asymptomatic samples using the PCR-cDNA Barcoding Kit (SQK-PCB111.24). Total RNA (500 ng) with an RNA IQ score >8 was used for strand-switching reverse transcription with Maxima H Minus Reverse Transcriptase and a poly(A)-targeting adapter. Unique barcodes were added during PCR amplification using LongAmp Hot Start Taq 2X Master Mix to enrich full-length transcripts. The amplified cDNA was purified with AMPure XP beads, quantified using Qubit and Agilent assays, and pooled in equimolar amounts. Sequencing adapters were then ligated using the Native Barcoding Kit 96 V14 (SQK-NBD114.96) for library preparation.