title: 16S metabarcoding of Artemisia-related samples
design: DNA was extracted using the DNeasy Plant Pro Kit (QIAGEN, Germany) from 100 mg ground tissue of root and shoot following the manufacturers instructions. For bulk and rhizospheric soils, 250 mg of soil was processed using DNA extraction with the DNeasy PowerSoil Pro Kit (QIAGEN, Germany). Then, bacterial 16S rRNA genes were amplified from the V5-V6 regions using the primers CS1:799F - CS2:1115R. Then, PCR reactions were performed in a final volume of 25L containing 12.5L Platinum Direct PCR Universal Master Mix (ThermoFisher, Temara, Morocco). PCR products were purified using AMPure XP beads (Beckman Coulter, USA) washed twice with ethanol, air-dried and resuspended in 10 mM Tris (pH 8.5).  A secondary PCR reaction with unique indexing primers (Fluidigm, Markham, ON, Canada) was performed to add sample-specific barcodes and sequencing adapters. Sequencing was performed on al Illumina Miseq platform (MegaFlex, Casablanca, Morocco) using MiSeq Reagent Kit V3 (600 cycles).