title: GSM9055773: PfHP1-DiCre ER rep3; Plasmodium falciparum; RNA-Seq
library_construction_protocol: Ring stage (~10 hours post infection, hpi) parasites were double sorbitol synchronized by 5% sorbitol. The samples were spun down and harvested using 0.015% saponin. The cell pellet was resuspended in Trizol and isolated by Isoprapanol precipitation. The isolated RNA was subjected to polyA enrichment using NEB polyA mRNA magnetic isolation module (E7490). The library preparation was done using the NEB Ultra II RNA kit (E7770). And  for the DiCre line samples NEBNext Ultra™ II Directional RNA Library Prep with Sample Purification Beads (E7765) was used for the library preparation. The library quality was tested using a DNA high sensitivity kit in Agilent bioanalyzer. The library amount was estimated using Qubit DNA high sensitivity kit. A final pool of 1.7 pM library was loaded on a 75 cycle High output illumina sequencing kit.