title: GSM8601702: 7298-JF-5; Homo sapiens; RNA-Seq
library_construction_protocol: Half of a 6 mm biopsy was cryopreserved in CryoStor CS10 media (BioLife Solutions). Samples were thawed on ice, washed briefly in cold Hank's Balanced Saline Solution (Gibco, Thermo Fisher Scientific) to remove residual CryoStor CS10 media, and bisected before being enzymatically digested in either 0.25% Trypsin-EDTA (Gibco, Thermo Fisher Scientific) with 10U/mL DNase I (Thermo Fisher Scientific) for 1 hour at 37°C, quenched with FBS (Atlanta Biologicals), or 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma) with 10U/ml DNase I in plain medium for 1.5 hours at 37°C with rotation. The resulting cell suspensions were filtered through 70-μm cell strainers twice and re-suspended in PBS containing 0.04% BSA. Dermal and epidermal cells were combined in a 2:1 ratio. libraries were constructed by the University of Michigan Advanced Genomics Core on the 10X Genomics Chromium system with chemistry v2 and v3