title: RNA seq of Glycine max
design: MaterialsWe cultivated Glycine max (GM) in a temperature gradient tunnel (3 m wide, 3 m high, 27 m long), which created a temperature gradient from the outside to the farthest end. GM plants were exposed to higher temperatures for an extended period of one week at the National Institute of Horticultural and Herbal Science, Eumseong, Republic of Korea. mRNA samples were collected from GM leaves at temperature increments of 0-1, 3, 4-5, and 7 degree higher than the outside temperature, labeled as T1, T2, T3, and T4, respectively.MethodsmRNA was extracted using TRIzol, and RNA sequencing was performed by Macrogen.