title: Fecal Microbiome sample of Cancer Patients Undergoing Immune Checkpoint Blockade
design: Extracted DNA was shipped frozen overnight to Diversigen, Inc. to undergo Illumina library prep and sequencing. Library preparation was adapted from the Illumina Nextera DNA Flex protocol, where genomic DNA was tagmented using bead-linked transposomes, followed by low cycle number PCR (5-8 cycles) to attach unique dual (UD) indexes. The libraries were pooled and sequenced on the NovaSeq6000 platform using the 2x150 bp paired-end protocol on an S4 flow cell. The instrument yielding between 7.5-12 Gb of data per subject sample.