title: RNA-seq of older human skeletal muscle following placebo or acipimox
design: ; fragmented mRNA was synthesized into first strand cDNA using random primers; second strand cDNA was synthesized with dUTP instead of dTTP; synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments; Digest the U-labeled second-strand template with Uracil-DNA-Glycosylase (UDG) and perform PCR amplification; Library quality control; Library circularization; The library was amplified to make DNA nanoball (DNB); Sequencing on DNBSEQ (DNBSEQ Technology) platform