title: GSM9055566: AsPC1, KDM4C KO1, H3K27Ac, rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: AsPC1 parental and KDM4C knockout cells were crosslinked with 1% formaldehyde for 10 min at room temperature, followed by quenching with 125 mM glycine for 5 minutes.  Chromatin was prepared by sonication to generate DNA fragments ranging from 200-600bp. ChIP was performed overnight using DiaMag Protein A-conjugated antibodies against H3K27ac (ab4729, Abcam), and H3K36me3 (ab9050, Abcam).   Immunocomplexes were collected the following day using DynaMag, then washed, and reverse crosslinked overnight, followed by DNA extraction. Libraries were prepared using NEB Ultra II DNA library prep kit (New England Biolabs), according to the manufacturer's protocol.