title: GSM8824327: NACA_SeRP_from_sucrose_cushion_IP_translatome_replicate1; Homo sapiens; OTHER
library_construction_protocol: RNA from ribosome fractions was extracted using hot acid phenol extraction as described in Günnigmann et al. 2023. For samples 5-14, ribosome profiling libraries were prepared as described in Günnigmann et al. 2023. For samples 1-4, ribosome profiling libraries were prepared using the NEXTflex Small RNA-Seq Kit v3 (PerkinElmer).