title: Illumina RNA sequencing of Sepia officinalis, skin (anterior lateral dorsal)
design: RNA was isolated from 5 mg of flash-frozen tissues (brain, skin and retina). First, tissue samples were homogenized in TRIzol using a homogenizer (VDI12/S12N-5S, VWR, Germany) following DNAseI treatment and column purification according to the Direct-zol RNA Micro prep kit from ZymoResearch (R2062). The RNA integrity and quantity were measured with the Qubit fluorometer (Invitrogen, Q33216) and the 2100 Bioanalyzer (Agilent Technologies, Germany). RNA-seq libraries were prepared from 300 ng of total RNA, using the Illumina TruSeq stranded mRNA library prep kit (Cat:20020594) and IDT for Illumina xGen UDI-UMI Adapters (Cat:10005903). Libraries were sequenced on an Illumina NextSeq500 using Mid output reagents (300 cyc) with 145 bp paired-end reads.