title: GSM9561852: Human glioma tissue, MR3 treatment, Patient P1; Homo sapiens; RNA-Seq
library_construction_protocol: Frozen tumor tissues were pulverized in liquid nitrogen using DEPC-treated, autoclaved mortars and pestles. Approximately 0.5 mL STAT60 (AMSBIO) was added during homogenization, followed by an additional 0.5 mL to reach a final volume of 1 mL. Total RNA was isolated using chloroform-based phase separation according to the manufacturer's instructions. RNA pellets were resuspended in 20–40 μL nuclease-free water. RNA concentration and purity were assessed using a NanoDrop spectrophotometer. Samples with concentrations >50 ng/μL and 260/280 ratios >1.7 were considered suitable for sequencing. RNA-seq libraries were prepared by Novogene from high-quality total RNA samples following standard Illumina-compatible protocols. Poly(A) selection was used to enrich for mRNA, followed by fragmentation, reverse transcription, adaptor ligation, and PCR amplification. Libraries were quality-controlled and sequenced on an Illumina platform to generate high-throughput transcriptomic data.