title: Hi-C of Acrossocheilus parallens: sample 1
design: For genome scaffolding, Hi-C libraries were prepared from the muscle tissue of the same female individual and used to assist the PacBio genome assembly. The Hi-C library construction process included cell crosslinking, cell lysis, chromatin digestion with MboI, biotin labeling, proximal chromatin DNA ligation, and DNA purification. All procedures were performed in accordance with previously reported standard experimental protocols. After quality control using an Agilent 2100 Bioanalyzer and qPCR validation, the qualified Hi-C libraries were sequenced on the Illumina Novaseq 6000 platform with a PE 150 bp mode.