title: RNA-Seq of Bacillus mojavensis: children skin
design: Sequencing was performed by Shanghai Meiji Biotechnology Co., Ltd. Genomic DNA was extracted using the Bacterial DNA Extraction Kit (GeneRay, Shanghai, China) following the manufacturers instructions. After quality control, sequencing was conducted using the second-generation Illumina platform and the third-generation PacBio Sequel IIe platform. Genome assembly, based on third-generation sequencing data, was performed using Unicycler v0.4.8. Coding sequences (CDSs) were predicted using Glimmer or Prodigal v2.6.3[18], while plasmid genes were identified with GeneMarkS[19], tRNA and rRNA predictions were conducted using tRNAscan-SE v2.0, and Barrnap v0.9 (https://github.com/tseemann/barrnap), respectively. Circular genome maps illustrating genomic features were generated with CGView software. Predicted protein-coding sequences were annotated via BLAST alignment against the COG, GO, KEGG, and CAZyme databases. Biosynthetic gene clusters (BGCs) associated with antibacterial compound synthesis in the VT275 genome were analyzed using the online tools AntiSMASH and Bagel4.