title: GSM8824039: Brg1-AID_CnR_MyoD-ER_4OHT_72h_T7; Mus musculus; OTHER
library_construction_protocol: CUT&RUN was performed using CUTANA™ ChIC/CUT&RUN Kit (#14-1048, Epicypher), following the manufacturer's instructions with the following modifications. 0.5 million cells were used for each CUT&RUN reaction. Cells were gently fixed with 0.1% formaldehyde for 1 min. Cells were resuspended in cold Nuclei Extract Buffer (20 mM HEPES pH 7.9, 10 mM KCl, 0.1% Triton X-100, 20% Glycerol, 1 mM MgCl2 supplemented with protease inhibitor and 0.5 mM Spermidine), lysed on ice for 10 mins, and centrifuged at 600 g for 3 min 4°C. Nuclei were resuspended in 100 uL cold Nuclei Extract Buffer and incubated with activated 10 uL/reaction Concanavalin A beads for 10 min at RT. The following steps are performed according to the manufacturer's instructions. Libraries were prepared following the instruction of CUTANA™ CUT&RUN Library Prep Kit for Illumina sequencing. CUT&RUN enriched DNA were used to construct libraries, using NEBNext® Ultra™ II DNA Library Prep kit (#E7645L, NEB) with AMPure XP magnetic beads (Beckman Coulter).