title: Bulk RNA sequencing data of the in vitro lifecycle of the kinetoplastid parasite Trypanosoma cruzi
design: Bulk RNA sequencing data of the in vitro lifecycle of the kinetoplastid parasite Trypanosoma cruzi
library_construction_protocol: Epimastigotes (EP) were collected in the exponential growth phase, from 2 days-old cultures initiated with 3x10/1^5 cells/ml. A mixture of stationary phase epimastigotes and metacyclic trypomastigotes (SE/MT) was obtained from a 10 days-old culture initiated with 3x10/1^5 epimastigotes/ml.  Total RNA was extracted using RNeasy Mini Kit (Qiagen, Hilder, Germany) following the manufacturer's protocol, with an on-column DNase digestion step with the RNAse-Free DNase Set (Qiagen).  Two biological replicates for each life cycle stage were prepared and sent to Novogene Co., Ltd (Cambridge, UK) for rRNA removal, library construction and sequencing. RNA quantity and integrity were measured with an Agilent 2100 bioanalyzer using the RNA Nano 6000 Assay Kit (Agilent Technologies, Santa Clara, CA, USA) and by agarose gel electrophoresis. A total of 1 µg total RNA per sample was used as input material for the lncRNA library preparation. Ribosomal RNA was depleted from total RNA and strand-specific libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (New England BioLabs, Ipswich, MA, USA) following manufacturer's recommendations. Index codes were added to attribute sequences to each sample.