title: GSM8790276: SLE, Donor 00171, NoTx; Homo sapiens; RNA-Seq
library_construction_protocol: Skin biopsies were transported to the laboratory on ice in Hanks Balanced Salt Solution (HBSS) buffer (Gibco) and processed immediately. Biopsies were incubated in 0.4% dispase (Life Technologies) in Hank's Balanced Saline Solution (Gibco) overnight at 4 degrees Celsius to separate epidermis from dermis. Digestion of the epidermis was performed in 0.25% Trypsin-EDTA (Gibco) with 10U/mL DNaseI (Thermo Scientific) for 1 hour at 37°C. Simultaneously, dermal samples were dissociated mechanically and then digested in 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma) with 10U/mL DNaseI in plain medium for 1.5 hours at 37 degrees Celsius. Following their respective incubation times, epidermal and dermal samples were strained through a 40uM mesh filter and centrifuged. Cells were subsequently mixed at a ratio of 1:1 before being submitted for single cell RNA sequencing. The 10x Chromium system with chemistry v3 was used by the University of Michigan Advanced Genomics Core to generate libraries, which were subsequently sequenced on the Illumina NovaSeq 6000 platform, generating 150-base pair paired-end reads.