title: ddRADseq of P. troodica: Pt67
design: RADseq libraries were constructed following the protocol described by Peterson et al. (2012), using 800ng of genomic DNA as starting material and two restriciton enzymes (SbfI and MspI) for DNA digestion. A combination of Illumina sequencing adapters and custom barcodes were used to tag each specimen. Size-selection of fragment in the range of 415-515 bp was performed with Pippin Prep.