title: Intra- and Inter-host Evolution of Human Norovirus in Healthy Adults
design: RNA was extracted from stool samples and RNA extracts were converted to cDNA using NEBNext RNA First Strand Synthesis Module and NEBNext Ultra II Directional RNA Second Strand Synthesis Module. Illumina unique dual barcodes adapters were ligated onto purified cDNA and libraries were amplified using the Kapa HiFi HotStart Library Amplification kit. Pooled cDNA libraries were hybridized with biotin-labeled probes from the HuNoV Panel (Twist Biosciences, Inc) at 70C for 16 hours. The post-capture pool was further PCR amplified for 12-20 cycles and final libraries were sequenced on Illumina NovaSeq 6000 S4 flow cell, to generate 2150 bp paired-end reads.