title: Single Amplified Genome sample from Haplosporidium nelsoni
design: To generate a reference resource for this parasite, we obtained a genome sequence from a single cell isolated from infected oyster host (Crassostrea virginica) hemolymph following panning-based enrichment. A microfluidic iBiochip device captured the single cell, and its genomic DNA was amplified using the QIAGEN REPLI-g Single Cell Kit (cat. #150343) with multiple displacement amplification (MDA) method. DNA quantity and purity (A260/280, A260/230) were assessed with Implen nanophotometer and Qubit 4 fluorometer (Thermo Fisher Scientific). MSX DNA was confirmed by qPCR (low Ct) for each sample prior to further analysis.