title: GSM8789814: HeLa cells, DMD ko, siUTRN, biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Total RNA Zol-Out™ (A&A Biotechnology) kit and RNA Clean & Concentrator™-5 with DNase I (ZYMO Research). 1000 ng/ul of total RNA was used for rRNA depletion. 2 ng/ul depleted RNA was used for the construcion of sequencing librabies. RNA libraries for RNA-seq were prepared using RiboCop rRNA Depletion Kit V2 (Lexogen) and RNA-Seq V2 Library Prep Kit with UDIs (Lexogen) following the Long Insert Sizes (RTL) manufacturer's protocols.