title: GSM9562076: MGS1 orthotopic model pooled replicate 1 (Control); Mus musculus; RNA-Seq
library_construction_protocol: Whole brains were grossly dissected and digested into single cell suspension using collagenase (20µl/ml; Roche) and DNase (1µl/ml; Roche) in RPMI 1640 medium (Roswell Park Memorial Institute; Corning) supplemented with 2% fetal bovine serum (FBS). The CD45+ population was isolated using Percoll and spiked 1:1 with CD45- before sequencing to obtain sufficient immune cells for profiling. scRNAseq library was constructed with the Chromium GEM-X Single Cell kit for a sequencing depth of 20,000 reads per cell. 20,000 cells were targeted for each sample