title: 16S rRNA gene amplicon sequencing of mouse fecal microbiome: ZT20H2
design: Genomic DNA was extracted from mouse fecal samples. The V3V4 region of the bacterial 16S rRNA gene was PCR-amplified and indexed libraries were prepared following standard amplicon library preparation procedures. Libraries were sequenced on an Illumina NovaSeq 6000 platform to generate paired-end reads, and raw demultiplexed FASTQ files were submitted to the SRA.