title: 16S rRNA V3V4 amplicon sequencing of enrichment 5
design: Liquid cultivation was performed using five different media, which were evaluated for their ability to enrich haloarchaeal communities: artificial seawater (ASW), Marine Broth (Difco) adjusted to 195g L-1 NaCl, R2A medium (HIMEDIA) at 3.12g L-1 prepared in 100% brine, a 1:1(v/v) mixture of brine and R2A (1.56g L-1), and brine supplemented with 0.1%(w/v) yeast extract and 0.075%(w/v) casamino acids. Each medium was inoculated with 5%(v/v) Lunenburg brine and incubated at 37C with shaking at 150rpm. After 32days, 2mL of each wellgrown culture were harvested by centrifugation (10min, ~14.100 g) and processed for amplicon sequencing as described in Section. All enrichments were performed in triplicates.