title: GSM9234441: CEA106S_rep_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from infected THP-1–derived macrophages using TRIzol reagent (Invitrogen, 15596018) followed by chloroform phase separation. The RNA was treated with DNase I (Sigma, AMPD1-1KT) to remove genomic DNA contamination. RNA integrity and purity were assessed prior to library preparation. Library preparation and sequencing were performed by BMKGene. Total RNA was enriched for mRNA using oligo(dT)-attached magnetic beads and fragmented. First- and second-strand cDNA synthesis was performed using random hexamer priming, followed by purification with AMPure XP beads. Double-stranded cDNA was end-repaired, adenylated, and ligated to sequencing adapters. Size-selected fragments (300–400 bp) were PCR-amplified to generate the final libraries. Library quality was assessed using Qubit 2.0 and Agilent 2100 Bioanalyzer. Sequencing was performed on an Illumina NovaSeq 6000 platform in paired-end 150 bp (PE150) mode by BMKGene.