title: RNA-Seq of Homo sapiens Rectal cancer derived organoids, unirradiated.
design: RNA was extracted from cells using the RNeasy Plus Mini Kit (Qiagen) according to the manufacturers protocol and at the indicated time points. Generation of sequencing libraries was performed using the Truseq RNA stranded poly A Library Preparation Kit (Illumina, San Diego, CA, USA). Sequencing was performed on an Illumina NextSeq2000 (1 x 50 bp). Sequencing reads were mapped to human reference GRCh37 using STAR (v2.7.3a).