title: GSM8600886: Donor P013, section 1; Homo sapiens; OTHER
library_construction_protocol: Tissue blocks containing the full span from pia to white matter were embedded in optimal cutting temperature compound (OCT) and stored at -80°C. The OCT-embedded samples were flash frozen in a 10×10 mm2 cryomold which was either frozen directly in liquid nitrogen or placed in a container of isopentane (Merck) which was in turn placed in liquid nitrogen at the same level as the isopentane.  10 μm-thick sections were cut from frozen blocks using a cryostat (Leica CM1860/CM1950) and collected onto the Spatial Gene Expression slide capture areas.  Hematoxylin and Eosin (H&E) staining was performed on Visium Spatial Gene Expression slides as detailed in the 10x Genomics demonstrated protocol guide CG000160 Rev B. Visium libraries were prepared from the stained tissue sections following the Visium Spatial Gene Expression Reagents Kit User Guide (CG000239, Rev D). At Step 1.1 the tissue was permeabilised for 12 minutes as determined using the Visium Spatial Gene Expression Tissue Optimisation User Guide (CG000238, Rev D). At Step 3.2, cDNA was amplified using 20 cycles. Visium Spatial Gene Expression Reagents Kit