title: 16s rDNA of wastewater
design: Purified PCR products were quantified by Qubit3.0 (Life Invitrogen) and every twenty-four amplicons whose barcodes were different were mixed equally. The pooled DNA product was used to construct Illumina Pair-End library following Illuminas genomic DNA library preparation procedure. Then the amplicon library was paired-end sequenced (2  300) on an NGS platform (Shanghai BIOZERON Biotech. Co., Ltd ) according to the standard protocols.