title: GSM8485737: Xenobot, day 7, sample 3; Xenopus laevis; RNA-Seq
library_construction_protocol: A sample of Xenobot consisted of 50 pooled Xenobots and each was repeated 3 times. A sample of age-matched Xenopus embryos consisted of 10 pooled stage 35 Xenopus embryos and each was repeated 3 times. Tissue was extracted using TRI Reagent (Molecular Research Center, Inc - MRC) as per the manufacturer's protocol, and total RNA quality and quantity was assessed using a NanoDrop spectrophotometer (Thermo Fisher Scientific). RNA library preparation was done with the Illumina Stranded Total RNA with Ribo-Zero Plus. Libraries were then multiplexed, and an rRNA depletion run using single-end, 75-nucleotide sequencing was performed on NextSeq 550 High Output 75 cycles.