title: Illumina NovaSeq 6000 paired end sequencing
library_construction_protocol: Oligo-dT beads were prepared using TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA, USA). Ligation was used to add the sequencing adapter and Illuminaï¿½s indexes and P5 and P7 was added with PCR for efficient sequencing. The libraries were sequenced on a NovaSeq6000 (Illumina, San Diego, CA, USA) and parameters were Paired End 150 base pairs (PE:150) with 8bp for the indexes.