title: Reduced Representation Bisulfite Sequencing of Sus scrofa: Brain Tissue from C4B
design: Genomic DNA was extracted from total DNA was extracted from Sus scrofa tissue using the DNeasy Blood & Tissue Kit (QIAGEN). DNAs were bisulfite treated using the EZ DNA Methylation-Gold Kit (Zymo Research). Reduced Representative Bisulfite Sequencing RRBS libraries were produced following the Nuggens Ovation RRBS Methyl-Seq System and 100 bp single end runs were sequenced on an Illumina NovaSeq platform in a NovaSeq S1 Flow-cell.