title: Stool metagenome of PBC patient
design: Stool samples were collected using additive-free mail-in kits returned to Mayo Clinic overnight on ice packs and stored at -80oC until further processing. The University of Minnesota Genomics Center performed DNA extraction using Qiagen's DNeasy 96 PowerSoil Pro QIAcube HT Kit. Fungal internal transcribed spacer 2 (ITS2) sequencing was performed on an Illumina MiSeq with 300 bp paired end reads.