title: RNA-Seq of chicken:ileum tissue
design: otal RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to themanufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop 2000spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed usingVAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturers instructions.