title: CK_3 (CRX743595; CRA011888)
library_construction_protocol: The mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase, and second strand cDNA synthesis was performed using DNA Polymerase I and dNTP. After blunt-end adaptor ligation PCR and purification, library quality was assessed on the Agilent Bioanalyzer 2100 system. Then, the clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina, San Diego, CA, USA), and the library preparations were sequenced on an Illumina Novaseq platform to generate 155 bp paired-end reads. submitted_instrument_model: Illumina NovaSeq 5000