title: RNAseq of Apis mellifera whole adult treated with uridine
design: Total RNA from whole adult was extracted. The RNA was then treated according to the SLAMseq protocol (Matsushima et al Nature methods 2019). Briefly, 5 ug of RNA were incubated with 10mM IAA and 50mM sodium phosphate in 50% v/v DMSO at 50C for 15mins. The RNA was subsequently purified and treated with 20 U of PNK for 30 mins. The RNA was subsequently purified and sent to the sequencing provider (Novogene) for library preparation and sequencing. Prior to library construction, RNAs of length between 17 and 100 nt were purified. The libraries were then prepared using a small RNA sequencing kit.