title: Illumina NovaSeq 6000 paired-end Sequencing of KAS22023238 (KAE22250350)
design: In order to understand the host-pathogen interaction in the early stage of infection, an in vitro culture passage model was constructed. RNA-seq analysis was performed by extracting RNA from PBMC cells 24 hours and 72 hours after MAP infection.
library_construction_protocol: Extraction of total RNA from bovine PBMC was performed by Qiagen RNEasy kit. Sequencing library was constructed with Illumina TruSeq Stranded mRNA Sample Preparation kit