title: HSM6_FLG_2 (CRX1175991; CRA018626)
library_construction_protocol: 1. Take a certain amount of total RNA samples, and use oligo dT beads to enrich mRNA with poly A tail;2. mRNA molecules were fragmented into small pieces;3. The fragmented mRNA was synthesized into first strand cDNA using random primers;4. The second strand cDNA was synthesized;5. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments;6. PCR amplification and purification;7. Library quality control;8. Library circularization;9. The library was amplified to make DNA nanoball (DNB);23. Sequencing on DNBSEQ (DNBSEQ Technology) platform.