title: Single cell RNA-seq of human GM12878 cells infected with Sendai virus and treated with aphidicolin against untreated control
design: Single cell RNA-seq of human GM12878 cells infected with Sendai virus and treated with aphidicolin against untreated control
library_construction_protocol: When cells are 80% confluent, aphidicolin or DMSO at 1uM concentration was added to the media and incubated for 12 hours at 37C. Sendai Virus or media was added to the media and incubated for additional 12 hours at 37C. Cells were collected at the end of 24h incubation. GM12878 cells were cultured with RPMI media supplemented with 10% FBS 37C with 5% CO2. When cells are 80% confluent, aphidicolin or DMSO at 1uM concentration was added to the media and incubated for 12 hours at 37C. Sendai Virus or media was added to the media and incubated for 12 hours at 37C. RNA extraction from each cell was performed according to 10x Chromium Single Cell 3' protocol. Library construction was performed according to 10x Chromium Single Cell 3' protocol.