title: GSM8601940: e2_dmso_chipExo1_aHA-EHMT2_rep2_20; Mus musculus; OTHER
library_construction_protocol: EHMT-dTAG ESC cells were cultured at 37˚C on feeders in FBS/LIF  conditions. Cells were collected by trypsinization for 3 mins and were washed with PBS followed by centrifugation (500 g, 5 mins, 4˚C). 10 M cells were used per each replicate of library. Pellet was resuspended to have 1 million cells per mL in PBS (RT). Cells were crosslinked with 1% formaldehyde in RT for 10 mins on platform shaker. Crosslinking was quenched with 125 mM glycine for 5 min at RT on platform shaker. Crosslinked cells were centrifuged (500 g, 5 mins, 4˚C) and was washed twice with ice cold PBS. After the final wash and centrifuge, the pellet was snap frozen before extraction. Frozen cell pellets were processed by the Lai lab at Cornell University, Ithaca as described previously in ChIP-exo 5.0 protocol (PMID: 30030442). Frozen cell pellets were processed as described previously in ChIP-exo 5.0 protocol (Rossi et al.).  anti-HA antibody (Abcam 9110) was used for o/n chromatin immunoprecipitation at 4˚C.Immunoprecipitated material was processed as described previously in ChIP-exo 5.0 protocol (Rossi)