title: GSM8791745: shRNA targets at Ahcy, no treatment, rep2; Drosophila melanogaster; RNA-Seq
library_construction_protocol: We affinity enriched photoreceptor nuclei for RNA isolation as previously described (Jauregui-Lozano et al., 2021, Genetics). Nuclear RNA was purified using ZYMO Direct-zol RNA Microprep Kits (#R2061). RNA library were prepared using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (E7760S). rRNA was depleted with lab-designed probes using NEBNext® RNA Depletion Core Reagent Set with RNA Sample Purification Beads (E7870L).  Libraries were sequenced using NovaSeq Plus.