title: RNA-seq of Marchantia polymorpha gemmae during dormancy release
design: RNA-seq of Marchantia polymorpha gemmae during dormancy release
library_construction_protocol: Dormant gemmae were collected from gemmae cups of adult thalli. Gemmae were sampled within a 5 minutes period for each of the three biological replicates. M. polymorpha sp. ruderalis was grown on soil under neutral day photoperiod (ND; 12/12; day/night). Plants were grown under cool white fluorescent light (50–60 µmol photons m−2 s−1) at 20°C constant temperature. RNA was extracted using an RNeasy Plant Mini Kit (Qiagen). Individual cDNA libraries were constructed using the TruSeq stranded mRNA library preparation kit with Poly-A selection (Illumina Inc.).