title: GSM9206155: MULTI_Etv3WT_GEX; Mus musculus; RNA-Seq
library_construction_protocol: Cell suspensions were enriched for DCs by negative selection. Briefly, sdLN cell suspensions were stained with biotinylated antibodies against TER-119, TCR-β, CD3, NK1.1, CD19, and Ly6G (Thermo Fisher Scientific and Biolegend), incubated with streptavidin microbeads (Miltenyi Biotec) and passed through LS columns attached to MidiMACS or QuadroMACS separators (Miltenyi Biotec) according to the manufacturer protocol. Cells in the flow-through were collected. After enrichment, ~21,000 Etv3+/+ and 54,000 Etv3-/- migratory DCs (migDCs) were sorted on FACSAria II (BD Biosciences) at the Cytometry & Cell Sorting Laboratory at NYUGSoM. migDCs = Live singlets lineage- (CD3- TCR-β- B220- CD19- NK1.1- NKp46- Siglec-F- Ly6G-) CD11c+ MHC-IIhi. Nuclei were isolated according to the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (GEX) protocol (10X Genomics) using an optimized 4 minutes of lysis. Libraries were prepared according to the 10X Genomics Single Cell Multiome ATAC Kit.