title: GSM9205728: WT, alpha-factor, Rep2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Total RNA was prepared using SDS, hot acid phenol, and chloroform Libraries for RNA-sequencing were prepared using "Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus" kits (with 50-nt sequencing) from total RNA. During the reverse-transcription step of library preparation, removal of cytoplasmic and mitochondrial rRNA was done using the QIAseq FastSelect -rRNA Yeast Kit.