title: 16S rRNA V4V5 amplicon sequencing of anaerobic digester sludge at THP 155C
design: Genomic DNA was extracted from anaerobic digester sludge. The bacterial and archaeal 16S rRNA V4-V5 region was amplified using primers 515F-Y and 926R. Amplicon libraries were prepared following the Ion Torrent protocol and sequenced on an Ion Torrent S5 system. Reads were processed using a DADA2-based workflow (rANOMALY) to infer amplicon sequence variants.