title: m6A_MeRIP_AD_2 (CRX755084; CRA012046)
library_construction_protocol: Total RNAs were extracted and fragmented to around 100 nt. The m6A-containing RNA fragments were incubated with Dynabeads protein A  pre-coupled with anti- m6A antibody. Following immunoprecipitation, the RNA-antibody-beads complex was washed thoroughly and then the RNA fragments were eluted from the beads through Proteinase K digestion. After incubation, the supernatant containing m6A-enriched RNAs were collected into a new tube and purified with RNA extraction solution followed by ethanol precipitation. Then, the purified RNAs were subjected to library construction with the SMARTer Stranded Total RNA-seq Kit v2. Sequencing was performed on Illumina Novaseq 6000 sequencing platform with paired-end 150 bp read length.