title: RNA-Seq of mus musculus: adult female coloncontent
design: This study aimed to investigate the effects of a novel treatment on gut microbiota composition in a mouse model. A total of 18 mice were divided into three groups: a normal control group (Control, n=6), a model group (DSS, n=6) with induced colitis, and a treatment group (QYD, n=6) receiving QYD after disease induction. Colonic content samples were collected post-intervention, and the V3-V4 region of the 16S rRNA gene was sequenced using an Illumina NovaSeq 6000 platform in 2250 bp paired-end mode.