title: GSM9205082: AW,striatum tissue,A53T; Mus musculus; RNA-Seq
library_construction_protocol: Mouse midbrain and striatum sites were taken, washed three times with pre-chilled Hanks Balanced Salt Solution (HBSS), and then cut into 2-3 mm blocks. After digestion, the tissue blocks were spin-digested with GEXSCOPE® Tissue Dissociation Solution (Singleron) at 37°C for 2.5 hours; they were then filtered through 70 μm and 40 μm sterile filters and centrifuged at 360 × g for 5 minutes. Subsequently, the supernatant was discarded, and the cell pellet was suspended in 600 µL of phosphate-buffered saline (PBS, HyClone). Samples were stained with Tissue Blue (Biorad, USA) and cell viability was estimated using a phase contrast light microscope (Nikon, Japan).Single-cell suspensions with 1×105 cells/mL in concentration in PBS (HyClone) were prepared. Single-cell suspensions were then loaded onto microfluidic devices and scRNA-seq libraries were constructed according to Singleron GEXSCOPER protocol by GEXSCOPER Single-Cell RNA Library Kit(Singleron Biotechnologies) .Individual libraries were diluted to 4nM and pooled for sequencing. Pools were sequenced on Illumina HiSeq X with 150 bp paired end reads.