title: Genome resequencing of Phaffia rhodozyma: M1-3
design: Briefly, genomic DNA of the ARTP mutants M1-3 was fragmented to 350500 bp using a Covaris 220 ultrasonicator (Covaris, USA). Following gDNA fragmentation, a genomic DNA library was constructed using TruSeq DNA LT Sample Prep Kit (Illumina, USA), which included end repair, adapter ligation, and 8-cycle PCR amplification. Sequencing was performed by Illumina X Ten, yielding 1.38 Gb raw data.