title: GSM9252873: RNASeq7_T4_sgROSA; Mus musculus; RNA-Seq
library_construction_protocol: For each starting sample, cells were lysed and 1 µg of total RNA was isolated for RNA-Seq while 9 µg of total RNA was digested with RNase I to isolate ribosome protected RNA footprints (RPFs). Both sample types were taken through rRNA depletion and RNA-seq samples were heat fragmented. Both sample types then underwent adapter ligation. Ligated RNA was reverse transcribed, libraries were generated via PCR, and then sequenced on a NextSeq 2000.