title: GSM9235064: PRC1-null HFSC H2AK119ub Cut&Run-1; Mus musculus; OTHER
library_construction_protocol: 120,000 FACS-enriched quiescent HFSCs were isolated from the back skin of P55 C57BL/6 wildtype mice. Cut&Run was conducted using the CUTANA™ ChIC/CUT&RUN Kit (Epicypher, 14-1048) following a previously described protocol optimized for FACS-isolated epidermal keratinocytes. Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) and NEBNext Multiplex Oligos (NEB, E6609L) for Illumina following the manufacturer's instructions. PCR-amplified libraries were purified using 1 X ratio of Agencourt AMPure-XP Beads (Beckman) and eluted in 15μl TE buffer.