title: GSM9528944: Breast cancer serum sample 20664-010, control; Homo sapiens; miRNA-Seq
library_construction_protocol: Total RNA was extracted from 200 µL of serum using the miRNeasy Serum/Plasma Kit (Qiagen) according to the manufacturer's instructions. Raw FASTQ files were generated for each sample. Reads were processed using Cutadapt for UMI correction, Bowtie2 for alignment to Homo sapiens miRNA sequences from miRBase v22.1, and edgeR (Bioconductor) for differential expression analysis. Counts were normalized using the Trimmed Mean of M-values (TMM) method and log₂-transformed to obtain ΔCq values. Sequence annotation was performed against the GRCh37 human reference genome and miRBase v22.1.