title: RNAseq of HnRNPF-WT-1
design: Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturers protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V10 RNA-seq Library Prep Kit (Premixed Version) according to the manufacturers instructions.