title: MAHD-383
design: "V4 region of 16S rRNA amplified with 515F/806R style primers; dual indexing per Kozich et al.; libraries cleaned and normalized with SequalPrep; pooled plates; quantified by KAPA qPCR; MiSeq v2 chemistry 2x250 with 1015% PhiX; demultiplex per IEM sample sheet."