title: GSM8651520: CD8, EPZ, 8days, biol rep2, INP; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% methanol free formaldehyde for 10 minutes & quenched with 125 mM glycine for 5 minutes. Quenced cells were lysed in 50 mM Tris-HCl pH8, 10 mM EDTA pH8, 1 % SDS + protease inhibitor cocktail, and sonicated for 3 minutes (30sec on, 30 sec off) on the Bioruptor PICO. Yeast chromatin was spiked in (1:1000 based on DNA concentration) prior to immunoprecipitation to allow for downstream normalization. The library was prepped using the KAPA genomic DNA prep Hyper, purified and quantified using the Bioanalyzer DNA 7500.