title: GSM9564862: Sperm from HFD, replicate 2; Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent and 1 µg of RNA was used for sequencing library construction. Mature sperm were isolated from epididymis and three individual sperm samples were pooled to generate one biological replicate. RNA libraries for smallRNA-seq were prepared using TruSeq Small RNA Library Prep Kit following manufacture's protocols.