title: GSM8683149: pituitary gland, anti-CD115 depletion, biol rep 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated with RNeasy Plus Micro Kit (Qiagen) according to the manufacturer's protocol and analyzed on Advanced Analytical Fragment Analyzer for quality assessment. A starting amount of 100 ng total RNA was used for libary construction. Library preparation was performed using the llumina Stranded mRNA Library Preparation Kit (1000000124518) according to the manufacturer's protocol. The quality of the samples was ensured using Advanced Analytical Fragment Analyzer. Sample concentration was measured with Qubit Fluorometric Quantitation (Life Technologies).