title: GSM9162634: iPSC-derived neural progenitors, trisomy 21, Cell line C13, Library C13_P7_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from ~2-10 mg of fetal human brain tissue using the RNeasy Plus Micro kit (Qiagen) according to manufacturer's instructions. In vitro differentiated NPC or neuron cultures (neurons matured to day 30) were dissociated with Accutase, pelleted, and flash frozen. Samples were stored in -80C until further processing. Library preparation and bulk-RNA-seq were performed using AccuraCode® RNAseq Kit (Singleron Biotechnologies).