title: GSM8683243: day 7 Dox treatment; Homo sapiens; RNA-Seq
library_construction_protocol: Samples were collected on days 1, 3, 5, and 7 following the initiation of Dox treatment. Single cells were cryopreserved in DMEM (Gibco) supplemented with 10% heat-inactivated FBS and 10% DMSO. Prior to sequencing, the cells were thawed, and dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec). An RNA library was generated using the 10x Genomics Chromium Single Cell 3' Library and Gel Bead Kit v3.1, and the amplified cDNA was sequenced on an Illumina NovaSeq 6000 S2 flow cell.