title: DNA-Seq of sponge metagenome: Petrosia ficiformis
design: Petrosia ficiformis was collected by SCUBA in January 2014 at depths of 27.3, from the Achziv nature marine reserve, Mediterranean Sea, Israel. The sponge was collected in compliance with permit 40246/2014 from the Israel Nature and National Parks Protection Authority. Only cortex tissue was used for further DNA extraction. The microbial DNA fraction was enriched using New England Biolab's NEBNext microbiome DNA enrichment kit.Genomic DNA was fragmented by sonication using Covaris S2 (Covaris, Woburn, MA). DNA libraries were prepared using the KAPA Hyper DNA library preparation kit with further Pippin Prep (Sage Scientific) size selection to 800 to 1,000bp. Low-quality (minimum-quality threshold = 20) and short (minimum-length threshold = 50) reads and reads with ambiguous bases (N) were trimmed with the software sickle version 1.33, using a sliding-window approach