title: GSM9258379: AdipoqF2KO mice sample 3; Mus musculus; RNA-Seq
library_construction_protocol: Jejunal mucosal scrapings were collected immediately following euthanasia by scraping the luminal surface of opened jejunum segments on ice. Tissue samples were flash-frozen in liquid nitrogen and stored at −80 °C until RNA extraction. Total RNA was extracted using TRIzol Reagent (Life Technologies) following the manufacturer's protocol. Phase separation was performed using chloroform, and the aqueous RNA phase was recovered and purified using the RNeasy Mini Kit (Qiagen). RNA integrity was confirmed by bioanalyzer before sequencing. RNA libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, which includes poly-A selection to enrich for mRNA. Libraries were sequenced on an Illumina NovaSeq X Plus system using single-end 75bp reads according to manufacturer protocols.