title: bulk RNAseq sample for subject A004 post tick removal
design: Samples were homogenized under liquid nitrogen using a mortar and pestle. Tissues were then resuspended in 1 mL of lysis buffer from the RNeasy Fibrous Tissue Mini Kit (Qiagen) and processed as per the manufacturer's protocol. mRNA was captured through rRNA depletion and used for RNAseq libraries, which were seqenced with 50bp single end reads on a HiSeq 2500