title: GSM8508621: BB010162; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA extracted from plasma samples of W. bancrofti-infected (n=20) and normal healthy volunteers (n=5) using the Norgen Plasma/Serum RNA Purification kit. After checking for quality control using Eukaryote total RNA Pico kit on Agilent BioAnalyzer 2100, barcoded Illumina TruSeq stranded total RNA libraries were generated for paired end sequencing using NextSeq's HiSeq3000/4000 platform with a read length of 76 (2 x 76 cycles). All samples had 20-28 million pass filter reads with more than 95% of bases above the quality score of Q30.