title: Nanopore barcoding of Acari and Collembola
design: Amplicon sequencing of 6,016 individually processed soil arthropod specimens, including 5,609 mites (Acari) and 407 springtails (Collembola), along with 128 negative controls. Each specimen was PCR-amplified using indexed primers targeting a ~650 bp fragment of the mitochondrial COI barcode region. Amplicons were subsequently pooled into a single library and sequenced on an Oxford Nanopore GridION platform (Oxford Nanopore Technologies). Basecalling was performed using Dorado, and reads are provided as a single FASTQ file prior to demultiplexing. Information required for demultiplexing and for linking sequences to individual specimens, including primer indices and specimen-level metadata, is available in Dryad (https://doi.org/10.5061/dryad.j9kd51ct5).