title: GSM8653733: EKD_Glc_IEVs; Mus musculus; RNA-Seq
library_construction_protocol: NovoNGS® CUT&Tag 4.0 High-Sensitivity Kit (for Illumina®)(Cat. No.:N259-YH01) After quality inspection, cell concentration of the single-cell suspensions was adjusted to 200 cells/μL, and then single cell capture and library construction were performed according to the instructions of the DECODER single cell 3' kit v1.0（Dynamic Biosystems, 2203106, 2203206, 2203306, 2203406）. The cDNA library was constructed with the v1.0 protocol of DECODER Single cell 3 'kit by Dynamic Biosystems (Suzhou, China). The library was quantitatively examined after fragmentation, adaptor ligation and sample index PCR.