title: 16S rRNA gene metabarcoding of Prorocentrum lima: Prorocentrum lima
design: Genomic DNA was extracted from all samples and used to amplify the V3?V4 region of the 16S rRNA gene using primers 341F and 923R with sample-specific barcodes. PCR was performed using Phusion High-Fidelity Master Mix, and products were purified via magnetic bead clean-up. Equimolar pooling was conducted, and sequencing libraries were prepared and quantified using Qubit and real-time PCR. Size distribution was assessed with a Bioanalyzer. Libraries were sequenced on the Illumina NovaSeq platform to generate paired-end reads. Adapter and primer sequences were removed prior to submission.