title: GSM9349909: MM134+vehicle; DNA repair; Homo sapiens; OTHER
library_construction_protocol: Single cell suspensions were prepared according to 10X Genomics recommendations. Briefly, cells were quickly washed with 0.25% trypsin (ThermoFisher) and then incubated in 0.25% trypsin for 5 min at 37°C. Trypsin digestion was quenched by the addition of cell culture medium. Cells were isolated by centrifugation at 150 × g for 3 min (these same conditions were used for all cell washes). Cells were washed twice with cold PBS containing 0.04% BSA. Cells were resuspended in 500 μl PBS with 0.04% BSA and filtered through a Flowmi™ Tip Strainer. RNA-Seq