title: GSM9137339: P1-Post_1, PanCK+; Homo sapiens; OTHER
library_construction_protocol: Blocks of formalin-fixed paraffin-embedded (FFPE) tumors were prepared for spatial transcriptomic analysis. One 5 µm section from each tumor specimen was placed on a glass slide for Nanostring GeoMx analysis (Seattle, WA). The slide was incubated with Ki-67 antibodies and the Regions of interest (ROIs) were chosen. ROIs were selected after histopathological review of tissue quality. ROIs were further selected for based on the presence of significant quantities of epithelial and immune cells, which was a pre-requisite to acquire significant RNA material from each cell type for subsequent spatial transcriptomic analysis. GeoMx Digital Spatial Profiling is a probe-based sequencing method to detect RNA spatially in tissue. We used the GeoMx Human Whole Transcriptome Atlas (18,816 genes) to sequence our tissue samples for the three patients.