title: GSM9382725: Liver_Treg_counts_GEX; Mus musculus; RNA-Seq
library_construction_protocol: Muscle and colon were enzymatically dissociated and digested with collagenase II/dispase to single cell suspensions. Tregs from hindlimb muscles and colons of Foxp3Gfp, Foxp3-cre x Maffl/fl (MafΔTreg)  and Foxp3-cre x Mafwt/wt (Maf-wt) were sorted by flow cytometry 3 days post CTX injury. Single cells were then encapsulated in nanodroplets according to the 10x Genomics protocol. 10X Genomics library prep (3' V2 or 5' V3) Library were sequenced using a HiSeq500.