title: GSM8627577: H9 cells,CRISPR/Cas9 NLRP7 1; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA of each of above listed samples was extracted using TRIzol reagent(Invitrogen, cat. NO 15596026) following the methods by Chomczynski et al (DOI:10.1006/abio.1987.9999). A amount of 2 μg total RNA per sample was used for stranded RNA sequencing library preparation using polyA selection method and KC-DigitalTM Stranded mRNA Library Prep Kit(Catalog NO. DR08502, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.