title: GSM9161719: GCM1 knockout organoids gene expression; Homo sapiens; RNA-Seq
library_construction_protocol: Single nuclei were isolated from organoids using the Chromium Nuclei Isolation Kit (10x Genomics) following manufacturer's protocol. Organoids were dissociated and nuclei were purified for multiome analysis. Approximately 10,000 nuclei per sample were processed for library preparation. Single-nucleus multiome libraries were prepared using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression kit (10x Genomics) according to manufacturer specifications. Approximately 10,000 nuclei were loaded onto a 10x Genomics Chromium Controller, enabling simultaneous profiling of chromatin accessibility and gene expression from individual nuclei. Libraries were sequenced on MGI DNBSEQ G400 with approximately 400M reads per lane across 2 lanes.