title: GSM9161546: sibling biol rep1; Danio rerio; RNA-Seq
library_construction_protocol: Embryos from tubgcp6+/- intercrosses were genotyped at 7 dpf to identify mutants and  siblings. Then, total RNA was extracted from tubgcp6 mutants and their siblings using Trizol reagent (15596026, Invitrogen) according to the manufacturer's instructions. The RNA sequencing libraries were constructed by NEBNext® UltraTM RNA Library Prep Kit for Illumina® RNA (NEB; E7770)  according to the manufacturer's instructions.