title: GSM9056213: kdrl:mCherry+ endothelial cells at 30 hours post fertilization crip2-/-;crip3-/-; Danio rerio; RNA-Seq
library_construction_protocol: kdrl:mCherry+ cells were isolated from transgenic fluorescent zebrafish embryos using FACS Aria. Sorted Cell populations were loaded on a Chromium Single Cell Controller (10X Genomics) to generate single-cell gel beads in emulsion (GEMs) by using  Single Cell 3' Reagents Kits v3.1 (10X Genomics). Captured cells were lysed and the released RNA was barcoded through reverse transcription in individual GEMs (Zheng et al., 2017). ScRNA-Seq libraries were prepared using Single Cell 3' library Gel Bead Kit v3.1 (10X Genomics) following the manufacture's instructions.