title: ATAC-seq analysis of ARID1A-deficient TOV112D human ovarian cancer cells treated with the G9a inhibitor BIX01294
design: ATAC-seq analysis of ARID1A-deficient TOV112D human ovarian cancer cells treated with the G9a inhibitor BIX01294
library_construction_protocol: TOV112D cells transduced with either shScramble or shARID1A were used to construct the ATAC-seq library. Libraries were assembled using the Omni-ATAC protocol26. Briefly, 50,000 cells were lysed in ice-cold ATAC-Resuspension Buffer for 3 minutes to isolate nuclei Libraries were assembled using the Omni-ATAC protocol26. Briefly, 50,000 cells were lysed in ice-cold ATAC-Resuspension Buffer for 3 minutes to isolate nuclei, which were then subjected to a transposition reaction with Tn5 transposase (Illumina, San Diego, CA, USA) at 37 â„ƒ for 30 minutes. After purification using the Zymo DNA Clean and Concentrator-5 Kit, the transposed DNA fragments underwent an initial amplification of five cycles, were quantified via real-time polymerase chain reaction, and were subsequently amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs).