title: GSM9163331: ATAC-iMGT-Myh6-eGFP-MEFs, siChst7, D1, BiolRep2; Mus musculus; ATAC-seq
library_construction_protocol: At Day 1 post-MGT overexpression, cells were harvested and frozen in 90% FBS with 10% DMSO using a controlled-rate freezing container (Mr. Frosty) at –80C. Approximately 250,000 cells were used per condition. Some cells were reserved for RNA extraction and sample validation. Assay for Transposase-Accessible Chromatin (ATAC) was performed using the Active Motif ATAC-seq Kit (#53150) according to the manufacturer's instructions. Following thawing, cells were lysed, and nuclei were subjected to Tn5 transposase-mediated tagmentation as described in the kit protocol. DNA clean up, library preparation and indexing were performed using reagents provided in the Active Motif kit. Final libraries were sequenced on the Element Biosciences AVITI platform with paired-end, 2×75 bp reads, targeting approximately 50 million reads per sample.