title: GSM9565685: IRE1-KO-IRE1-mNeonGreen + RocA + DOX (Rep3); Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Direct-zol RNA Miniprep Plus kit (Zymo Research) according to the manufacturer's protocols. The yield and the integrity of RNA were determined by the spectrophotometer NanoDrop 2000. Libraries were prepared according to the Watchmaker mRNA Library Prep Kits (Watchmaker Genomics 7BK0001-096) protocol, using 200 ng of RNA input, and sequenced on a NextSeq 2000 using XLEAP-SBS chemistry.