title: Whole genome HiFi sequencing of Aldabrachelys gigantea: Tank2
design: High Molecular Weight DNA Extraction (HMW DNA) was extracted from blood with the MagAttract HMW DNA kit (Qiagen) with minor modifications.  HMW DNA (8ug) were sheared on a Megaruptor 3 (Diagenode, NJ). Sheared DNAs were size selected for fragments 10kb to 20kb in a BluePippin (Sage Science, MA) using a 0.75% agarose gel Cassette.  The PacBio HiFi library was prepared from 2ug of size-selected DNA with the SMRTBell Express Template Prep kit 2.0.