title: GSM8684054: Homo sapiens, Human non-small cell lung cancer cells, knock out CHD6, biol rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from each sample using the TRIzol Total RNA Extraction Reagent Kit (Takara, Cat.No.DP424), with each sample yielding >2μg of total RNA. Libraries were constructed by APExBIO according to the manufacturer's instructions for Illumina library preparation. Briefly, polyA-tailed mRNA was captured using oligo(dT) magnetic beads, the captured mRNA was fragmented, and then reverse transcribed and converted to double-stranded cDNA. The cDNA products were purified using the AMPure XP system (Beckman Coulter, Beverly, USA).