title: GSM9381240: Kat6a WT proliferating, rep 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy mini kit (Qiagen, 74106) for proliferating cells or RNeasy micro kit (Qiagen, 74004) for differentiating cells according to the manufacturer's instructions, with the addition of the DNase I supplemental protocol.  RNA concentration and quality was analysed on the Agilent 4200 Tapestation using RNA tape (Agilent, 5067-5579). 200 ng of RNA per sample was used to generate stranded sequencing libraries using the TruSeq Stranded mRNA (100 ng plus kit, Illumina) according to the manufacturer's instructions. The Samples were barcoded, pooled and sequenced on a NextSeq2000 instrument (Illumina).