title: 18S rRNA amplicon sequencing of phytoplankton in the Bohai and Yellow Seas: seasonal surface seawater samples
design: This study aims to investigate the diversity and community composition of phytoplankton in the Bohai and Yellow Seas using 18S rRNA amplicon sequencing. Environmental water samples were collected from multiple stations across different seasons. Total DNA was extracted from the filtered plankton biomass, and the V4 region of the 66S rRNA gene was amplified using eukaryote-specific primers. The resulting amplicons were sequenced using the Illumina MiSeq platform with paired-end reads. This design allows for the assessment of temporal and spatial variations in phytoplankton community structure, providing insights into the ecological responses of marine eukaryotic plankton under changing environmental conditions.