title: RNA-Seq of subcutaneous tumors: MC38 tumor bearing mice treated with anti-PD-1 and MI37. 2
design: 1ug of total RNA was used to construct cDNA libraries with the TruSeq RNA library kit. The protocol consisted of polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription and 101nt paired-end sequencing by Illumina NovaSeq 6000. The libraries were quantified using qPCR according to the qPCR Quantification Protocol Guide and qualified using an Agilent Technologies 2100 Bioanalyzer.