title: 16S rRNA sequences
design: The forward 27F: AGRGTTYGATYMTGGCTCAG and reverse 1492R: RGYTACCTTGTTACGACTT universal primer set was used to amplify the full-length 16S rRNA gene from the genomic DNA extracted from each sample. Both the forward and reverse 16S primers were tailed with barcode sequences to allow for multiplexed sequencing.