title: A spatial map of human liver cirrhosis reveals the patho-architecture and gene signatures associated with cell state transitions during liver disease - Spatial Transcriptomics (10X Visium) validation cohort
design: A spatial map of human liver cirrhosis reveals the patho-architecture and gene signatures associated with cell state transitions during liver disease - Spatial Transcriptomics (10X Visium) validation cohort
library_construction_protocol: Liver tissue was obtained following ethical approval and informed consent (National Research Ethics Service, REC14/NW1260/22) from the Manchester Foundation Trust Biobank. Fresh, unfixed tissue was obtained intraoperatively from patients undergoing surgical liver resections at the Manchester Royal Infirmary. Tissue was snap frozen within 30 minutes of collection and stored at -80ËšC.  Used 10X Fresh Frozen Visium (ie polyA capture)  sample collection protocol, detailed in 03.CG000239_VisiumSpatialGeneExpression_UserGuide_RevC.pdf Used 10X Fresh Frozen Visium (ie polyA capture)  sample collection protocol, detailed in 03.CG000239_VisiumSpatialGeneExpression_UserGuide_RevC.pdf