title: GSM8757908: IZ/ACP organoids from hiPSCs (no TP); Homo sapiens; RNA-Seq
library_construction_protocol: ExpLBM cells were washed with PBS (−) and dissociated with accutase at 37°C for 5 min, while IZ/ACP and GPC organoids were washed with PBS (−) and then treated with 900 U/mL collagenase (type II) (Gibco) for 60 min at 37°C, followed by gentle pipetting using a serological pipette to dislodge and dissociate aggregates. Cells were sorted on a FACSAria III Cell Sorter (BD Biosciences) to remove dead cells and debris via forward scatter, side scatter, and DAPI/calcein-AM staining. A total of 10,000 cells were analyzed. A Chromium Next GEM Single Cell 3′ GEM Kit v3.1 (10x Genomics) was used for library preparation. Dissociated cells were separated into single droplets with unique molecular barcodes and then processed following the manufacturer's protocol. Quality checks of the samples were performed using high sensitivity D5000 screen tape (Agilent) and the Agilent 4150 TapeStation system (Agilent). Sequencing was performed using DNBseq (MGI).