title: GSM9532577: Acute Myeloid Leukemia Patient Blood Sample, PS_754422; Homo sapiens; RNA-Seq
library_construction_protocol: ChIP DNA: Primary tissue samples were cross-linked with 1% formaldehyde for 10 min at room temperature and quenched with 125 mM glycine. Nuclei were isolated, chromatin was sheared to ~200–500 bp using a Covaris E220, and immunoprecipitation was performed overnight at 4°C with anti-H3K79me2 (Abcam, ab3594). DNA was purified using the MinElute PCR Purification Kit (Qiagen). RNA: Total RNA was extracted using TRIzol (Invitrogen) followed by DNase I treatment. RNA integrity was assessed by Agilent 2100 Bioanalyzer and samples with RIN ≥ 7 were used. ChIP-Seq Library: ChIP DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced on an Illumina NextSeq 3000. RNA-Seq Library: Poly(A)+ RNA was enriched using oligo(dT) magnetic beads. Strand-specific libraries were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) and sequenced on an Illumina NextSeq 3000 (~50M reads per sample).