title: GSM8682841: endometrial stromal cells, E2_P57; Homo sapiens; RNA-Seq
library_construction_protocol: Medium for estrogen treatment was phenol red-free DMEM/F12 (1:1, vol/vol) medium (0% FBS- DMEM/F12) without FBS and with antibiotic-antifungal mixture solution (1:100, vol/vol). medium) was used; E2(Cayman chemical, Michigan)ethanol solution (10-5 mol/L), E4(Tront Research Chemicals, Canada) ethanol solution (10-3 mol/L) or EE(Pharmaceutical and Medical Device Regulatory Science Society of Japan, Japan) ethanol solution (10-6 mol/L) was diluted in 0% FBS- DMEM/F12 medium to obtain E2 (10-6, 10-8, 10-9, 10-10 mol/L), E4 ( 10-6, 10-8, 10-9, 10-10 mol/L), or EE (10-9, 10-11, 10-12, 10-13 mol/L) medium. The control was 0% FBS- DMEM/F12 medium without estrogen.　 Endometriosis cells were cultured for 3-48 hours in medium for estrogen treatment. RNA libraries for RNA-seq were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and  NEBNext Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.