title: GSM9347869: impdh2-0 knockdown 4 days after germination replicate 2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA was extracted from leaves  using the NucleoSpin® RNA Plant Kit (Macherey-Nagel) according to the manufacturer's instructions. 50 mg of fresh plant material was used for this purpose. The RNA was eluted from the column with 40 μl of RNase-free ddH2O Library construction was perfomed by Novogene Sequencing Service. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection