title: GSM9566597: tumor, untreated, BCR, rep1; Mus musculus; OTHER
library_construction_protocol: Tumors were minced into 2mm pieces and digested with collagenase A and a simultaneous 150rpm homogenizatino process for 30 min at 37°C. Cells were washed twice with cold PBS 2% FBS and centrifugated 5min at 400 x g to stop the lysis reaction. Single cell suspensions were counted and submitted to 5' single-cell RNA sequencing using the 10x Genomics Single Cell 5' v2.