title: This raw genome sequence corresponds to Desulforudis DRI-14, formerly known as Clostridia bacterium DRI-14.
design: The DNA was sheared to an average fragment length of 500 bp using a Covaris S220, according to the manufacturers recommendations, to construct a shotgun library for sequencing. A Nugen Ovation Ultralow Library construction kit was used for end repair, adaptor ligation, and amplification. Eighteen cycles of amplification were necessary to produce the amount of material required for sequencing. The resulting sample was sequenced on an Illumina MiSeq, using a 2x250bp paired-end kit, resulting in 10.6 million pairs (21 million individual 250bp reads) from 60% of a lane.