title: DR-O2
design: The sequencing libraries were generated with the low sample RNA input, SMART-Seq v4 Ultra Low Input RNA Kit was used according to the manufacturers instructions for the cDNA synthesis.  Amplified cDNA was purified using Agencourt AMPure XP bead kit. Sequencing libraries were prepared with the NexteraXT DNA Library Preparation kit (Illumina FC-131-1096) using IDT-ILMN Nextera DNA UD Indexes (96 Indexes). Each library was uniquely indexed and then sized and quantified by capillary electrophoresis (TapeStation, Agilent). The indexed libraries were normalized, pooled and then sequenced on an Illumina NovaSeq 6000 system.