title: RNA-Seq of Mus musculus:Lung
design: Total RNA was extracted from lung tissues collected on day 3 post-SARS-CoV-2 infection using the TRIzol reagent following the manufacturers protocol. RNA quality and quantity were assessed using an Agilent 2100 Bioanalyzer. mRNA was isolated using oligo(dT)-attached magnetic beads to capture polyadenylated transcripts. The mRNA was converted to first-strand cDNA using reverse transcription, followed by amplification to generate second-strand cDNA. Libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina, including adapter ligation and PCR enrichment steps. Final libraries were quantified and sequenced on an Illumina NovaSeq 6000 platform, generating 150 bp paired-end reads.