title: GSM9056159: MTA1_1; Homo sapiens; RNA-Seq
library_construction_protocol: The total RNA was extracted from PC-3 cells using TRIzol Reagent (Invitrogen, cat. NO 15596026) following the methods by Chomczynski. DNA digestion was carried out after RNA extraction by DNase I. RNA quality was determined by examining A260/A280 with NanodropTM OneCspectrophotometer (Thermo Fisher Scientific Inc). RNA Integrity was confirmed by 1.5% agarose gel electrophoresis. Qualified RNAs were finally quantified by Qubit3.0 with QubitTM RNA Broad Range Assay kit (Life Technologies, Q10210). The cDNA library was prepared according to the manufacturer's protocol, using the SMARTer Stranded Total RNA-Seq Kit version 2 (634413; Takara/Clontech). Sequencing was performed on the Illumina NovaSeq system with 150 nt paired - end reads.