title: GSM8631081: Brain, Sham-GFP, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Nuclei extraction was performed using the Miltenyi Nuclei Extraction Buffer (Miltenyi Biotec, Auburn, CA, USA) following manufacturer's guidelines with gentle MACS Dissociation and C tubes. Library preparation on isolated nuclei was performed with 10X Genomics Chromium Single Cell 3' kit v3.1, according to standard manufacturer's specifications.