title: GSM9678135: Micro-C of RIVA ARID1A Het ARID2 KO clone 2 rep 2; Homo sapiens; OTHER
library_construction_protocol: Two ARID1A wild-type and two heterozygous clones were used. Cells were crosslinked with 1% formaldehyde at room temperature for 10 min and quenched with 750 mM Tris-HCl (pH 7.4). Chromatin was digested with micrococcal nuclease. Digested DNA ends were repaired using biotinylated oligos and subjected to proximity ligation. For library preparation, biotynilated DNA was purified with streptavidin beads. For library preparation, the DNA was bound to MyOne Streptavidin C1 beads from Invitrogen, washed with TBW, and processed using the NEB Ultra II DNA Library Prep Kit. Libraries were amplified using 10 PCR cycles, quantified via BioAnalyzer and Tapestation, and then sequenced on the NovoSeq X Plus platform using the PE150 SP Kit.