title: Aged mouse, combination #3, Gastrocnemius
design: Total RNA was extracted from intestinal and muscle tissues using QIAzol Lysis Reagent and the RNeasy Mini Kit. RNA integrity was confirmed with RIN > 7.0. Libraries were prepared from 1 ?g of total RNA using the TruSeq Stranded mRNA Sample Preparation Kit. Paired-end (2 x 100 bp) sequencing was performed on the Illumina NovaSeq 6000 platform.