title: RNASEQ of homo sapiens: Melanoma biopsy collected prior to previous PD-1/L1 therapy (archival)
design: RNA was extracted from biopsies (stored in formalin-fixed paraffin-embedded blocks or slides) using the Covaris truXTRAC FFPE tNA Ultra kit. RNA was processed by cDNA hybridized capture for transcriptome profiling from 100ng of RNA on the Agilent BRAVO platform using the Agilent SureSelect XT HS2 RNA library preparation kit with the Agilent SureSelect Exome v7 capture probe. Libraries were pooled for a final concentration of 2nM and sequenced on the Illumina NovaSeq X platform (S4 flow cell, 2x150bp reads). Data is proved in the form of raw fastq files.