title: 16s rRNA of mus musculus: fecal sample
design: Genomic DNA from mouse feces was extracted via CTAB/SDS. The 16S rRNA V4 region was amplified with primers 515F/806R using Phusion High-Fidelity PCR Master Mix. Amplicons were bead-purified, pooled equimolarly, and gel-extracted. Libraries were prepared with the Illumina TruSeq DNA PCR-Free Kit and sequenced on a NovaSeq 6000 for paired-end reads.