title: GSM9427897: Immortalized periodontal ligament cells compressive force replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Direct-zol RNA Microprep Kit according to the manufacturer's instructions. RNA quality was assessed using a NanoDrop 2000 spectrophotometer. RNA libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit (Lexogen), a poly(A)-based method, with 500 ng of total RNA per sample, following the manufacturer's protocol.