title: GSM9164309: PPKS mouse pancreas, non-targeted sgRNA, survival endpoint, mouse 23830; Mus musculus; RNA-Seq
library_construction_protocol: total RNA was extracted from flash frozen pancreas tissues using Qiagen Allprep Mini kit (including optional on-column DNase treatment) Directional mRNA-seq libraries were generated using KAPA mRNA HyperPrep kit, libraries were single-indexed using Illumina TruSeq single index adapters. 800 ng total RNA was used to generate libraries. Libraries were sequenced on an Illumina NextSeq550 sequencer (75 cycles, single end)