title: GSM9381396: R1_Ctrl; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were loaded onto a Chip A Single-Cell Kit v2.1 (MobiDrop (Zhejiang) Co., Ltd., Cat. No. S050100301, Zhejiang, China) to generate droplets with MobiNova-100 (MobiDrop (Zhejiang) Co., Ltd., Cat. No. A1A40001). Each cell was prepared in a droplet that contained a gel bead linked with up to millions of oligos (cell unique barcodes). After encapsulation, the droplets are cut by light with a MobiNovaSP-100 (MobiDrop (Zhejiang) Co., Ltd., Cat. No. A2A40001), while the oligos diffused into the reaction mixture. The mRNAs were captured by cell barcodes with cDNA amplification in droplets. Following reverse transcription, cDNAs with barcodes were amplified, and a library was constructed using the High Throughput Single-Cell 3' Transcriptome Kit v2.1 (MobiDrop (Zhejiang) Co., Ltd., Cat. No. S050200301) and the 3' Dual Index Kit (MobiDrop (Zhejiang) Co., Ltd., Cat. No. S050300301). The resulting libraries were subsequently sequenced on an Illumina NovaSeq Xplus System. Generating the “water in oil” droplets based on the microflu-idics technology, by identifying the fluorescence signal in the droplets, and deflecting droplets to different channels using dielectrophoresis, thereby fulfilling highly efficient cell sorting.MobiNova-100 Single Cell System utilizes patented barcoding and microfluidic technologies to achieve reliable single cell partitioning and effective cell capture in minutes.