title: GSM9567084: B2KO.UTI.3; Mus musculus; RNA-Seq
library_construction_protocol: Bladders were homogenized in 500 µL TRIzol reagent (Thermo Fisher Scientific; #15596026) using a handheld homogenizer. Lysates were centrifuged at 17,000 x g for 5 min at 4 °C. RNA was purified from 450 µL lysate using the Zymo Direct-zol RNA Miniprep Kit (Zymo Research; R2050) according to the manufacturer's protocol. RNA concentration and purity were assessed by ThermoScientific Nanodrop One (cat# 13-400-525). Sequencing was done on a NovaSeq X Plus, producing paired end 150bp reads.