title: GSM9566325: Wildtype neural progenitor cells R4; Homo sapiens; RNA-Seq
library_construction_protocol: Each replicate was harvested from one 100% confluent 10 cm culture dish, comprising approximately 3-5x10^6 cells. Cells were first washed with ice-cold 1x PBS, before scraping them off the dish with ice-cold 900 µL TRIzol for resuspension. Cell suspensions were immediately transferred to RNase-free 1.5 mL tubes and stored at -80 °C and handed over to Gene Center (LMU Munich) for RNA extraction, library preparation, and RNA sequencing. RNA extraction and library preparation were performed using the SMARTer Stranded Total RNA-Seq Kit v3 (Takara).