title: Bombus haematurus Whole Genome Sequence
design: 1 microgram DNA was fragmented using Covaris (Covaris, LLC). 200-400 bp fragments were selected using magnetic beads. End repair and 3' adenylation was carried out, followed by adapter ligation. PCR amplification and circularization was carried out. The single stranded circular DNA was used as the library. After quality control, the library was amplified using phi29, creating DNA nanoballs. These were loaded into patterned nanoarray and sequenced using combinatorial Probe-Anchor Synthesis, on a DNBSEQ-G400 platform(MGI Tech)