title: FL-cDNA for pachytene
design: Samples from vegetative growth, pachytene, and metaphase II stages were collected. Total RNA was extracted and quality-checked, then reverse-transcribed into cDNA. Sequencing libraries were prepared by adapter ligation using the Oxford Nanopore SQK-PCS109 PCR-cDNA Sequencing Kit. The final cDNA libraries were loaded onto an Oxford Nanopore PromethION sequencer for sequencing.