title: rpd3 complemented with catalytic dead RPD3
design: Cell pellets for genomic DNA (gDNA) isolation were harvested through pelleting 1 mL of culture via centrifugation at 12000 rpm. Cells were washed and resuspended in 1X TE before being subjected to DNA extraction using the Qiagen Gentra Puregene Yeast/Bacteria kit (158567) as previously described [20]. Purified gDNA was treated with RNase A before being quantified and submitted for library preparation using the Illumina DNA prep kit and Whole Genome Sequencing (WGS) at SeqCenter. Paired-end (PE) sequencing was performed using an Illumina NovaSeq X Plus at SeqCenter.