title: Genome-wide gene expression profiling in regenerating ear pinnae in BALB/c mice induced with pharmacological epigenetic therapy involving subcutaneous administration of zebularine and retinoic acid in alginate hydrogel
design: Genome-wide gene expression profiling in regenerating ear pinnae in BALB/c mice induced with pharmacological epigenetic therapy involving subcutaneous administration of zebularine and retinoic acid in alginate hydrogel
library_construction_protocol: The tissues were collected 7 days after the injury, placed immediately in liquid nitrogen and stored at -80Â°C.  Total RNA was extracted from 3 mm rings surrounding the initial punch wounds using RNeasy Mini kit (Qiagen). The quantity of the extracted RNA was determined fluorometrically on Qubit RNA assay (ThermoFisher Scientific), while the RIN (RNA integrity number) was determined using RNA Screen Tape Analysis on 4150 TapeStation System (Agilent). TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat kit (Illumina) was used for library construction, followed by 5' and 3' adapter ligation.