title: Genome of methanococcoides sp. isolate SH24
design: The starting material is DNA (100 ~ 1000ng) passed quality control. DNA is fragmented by acoustic disruption using Covaris S220 and then undergoes end repair, dA-tailing, adapter ligation and purification. The purified DNA is further selected for the right size before PCR amplification for library construction. The preliminary quantification and dilution of the library is performed using Qubit3.0, and then Agilent 2100 was used to determine the inser  size and nucleic acid concentration of the resulting library sample. The effective concentration of each sample library in the library mixture is determined by qPCR before sequencing to ensure the accuracy of the sample concentration and the reliability of the sequencing data. A schematic view of library construction is shown below