title: Amplicon sequencing of microbial communities: controls
design: Control sample. Total DNA was extracted using the NucleoSpin 96 Soil kit (Macherey-Nagel). Amplification of the 16S V3-V4 (341F/785R) region was performed based on 2-step PCR procedure (with 28 and 8 cycles, respectively). Illumina Miseq with 300 bp paired-end reads (v3 reagents).