title: RNA-seq of malignant pleural mesothelioma cell line MSTO-211H upon silencing of lncRNA linc00941
design: RNA-seq of malignant pleural mesothelioma cell line MSTO-211H upon silencing of lncRNA linc00941
library_construction_protocol: Cells were collected after 72 hours from transfection. MSTO-211H were cultured in RPMI GlutaMax medium added with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Cells were grown at 37°C, 5% CO2. cells were reverse-transfected with siRNA against linc00941  or Negative Control siRNA at a final concentration of 20 nM. Total RNA was purified with Maxwell® RSC simplyRNA Cells (Promega). RNAseq libraries were prepared by Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit starting from 500 ng of RNA. RNA quality was assessed by Bioanalyzer RNA 6000 nano kit.