title: GSM9055818: Sample GSF-F-138 treated with Etoh for 72h; Homo sapiens; RNA-Seq
library_construction_protocol: The cells were lysed in RNA-extraction buffer, and total RNA was extracted using the RNeasy Mini Kit with on-column DNase digestion. 1μg of total RNA was used for the construction of sequencing libraries. RNA libraries for the mRNA sequencing were prepared using the Illumina Stranded mRNA Prep Ligation kit following the manufacturer's protocol.