title: experimental wetland soil
design: experimental wetlands system rhizosphere Soil DNA was extracted from the rhizosphere soil using the CTAB method and quantified before PCR amplification. Specific primers were used for targeted amplification. PCR involved 32 cycles of denaturation, annealing, and extension, and the products were verified by agarose gel electrophoresis. Rhizosphere samples for 16s rDNA were sequenced on the Illumina NovaSeq platform and paired-end reads were processed and quality-filtered using FLASH and fqtrim. Chimeric sequences were removed with Vsearch, and feature tables were generated with DADA2.