title: 16S bacteria DNA seq: SERC BiodiversiTREE plot 66
design: DNA was extracted from homogenized soil samples using the PowerSoil Pro DNA extraction kit. Samples were then processed with metabarcoding amplification and Illumina NovaSeq Sequencing through the University of Tennessee, Knoxville Sequencing Core facility. The primers used for 16S rRNA bacterial genes were S-D-Bact-0341- b-S-17/S-D-Bact-0785-a-A-21 and S-D-Bact-0008-a-S-16/S-D-Bact-0907-a-A-20