title: Transcriptome of Cucurbita moschata: Seed Coat at 15 DAP
design: Seed coat tissue of Cucurbita moschata was collected at 15 days after pollination (15 DAP). Total RNA was extracted and used for cDNA library construction with the Fast RNA-seq Lib Prep Kit V2 (Cat. No. RK20306). Libraries were sequenced on an Illumina NovaSeq X Plus platform with paired-end reads. The C. moschata reference genome (http://cucurbitgenomics.org/v2/ftp/genome/Cucurbita_moschata/v1/) was used for alignment with HISAT2 v2.2.1. Transcript quantification was performed using FeatureCounts, and differential gene expression analysis was conducted with DESeq2 v1.42.0.