title: WGS of Homo Sapiens
design: Genomic DNA was quantified using a Qubit 4.0 Fluorometer (Thermo Fisher Scientific). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturers protocol. DNA was fragmented by acoustic shearing (Covaris LE220), followed by end repair, 3 adenylation, adapter ligation, and limited-cycle PCR enrichment. Libraries were validated using an Agilent TapeStation and quantified by Qubit fluorometry and qPCR (KAPA Biosystems).