title: GSM9210437: AL2PX; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using Agilent 2100 RNA Nano 6000 Assay Kit. 1.3 ug of total RNA was usedfor the construction of sequencing libraries. Polyadenylated (polyA+) mRNAs from eukaryotic samples were enriched using oligo(dT) magnetic beads. Purified mRNA was fragmented by incubation with Fragmentation Buffer. First-strand cDNA synthesis was then performed using fragmented RNA as template with random hexamer priming. Second-strand cDNA synthesis was subsequently carried out using Second Strand Marking Buffer and Second Strand/End Repair Enzyme Mix. The resulting double-stranded cDNA underwent end repair, 3′-end adenylation, and adapter ligation. Size-selected fragments were purified using magnetic beads, followed by PCR amplification to complete library preparation.