title: RNA-seq of Embryonic Chilean Tinamou Forelimb Bud (HH18)
design: RNA was extracted using the standard protocol for the PicoPure RNA Isolation kit (Arcturus). All total RNA was diluted to 2 ng/uL and 50 uL were provided to the Harvard Bauer Core for Kappa Hyperprep RNA-seq Library synthesis with poly-A selection (Roche).  Insert sizes of 200-300 were requested and 13 cycles of PCR utilized.  All libraries were sequenced on a Nextseq High 150 (Illumina) as paired-end 37bp reads.