title: GSM9162383: Pig P3 Skin, scRNA-seq; Sus scrofa; RNA-Seq
library_construction_protocol: scRNA-seq: Epidermal and dermal cells were isolated from E90, P3, P10, or 6-month old pig skin as previously described (Jensen et al., 2010), with the addition of Elastase (Worthington Biosciences), Hyaluronidase (Sigma-Aldritch), and Collagenase IV (Worthington Biosciences) enzymes to the dermal digestion solution to obtain single-cell suspensions of unsorted epidermal and dermal cells. Stereo-seq: Fixed-frozen cryo skin samples from P3, P10, and 6-month old pigs were sectioned at 10um for use with Complete Genomics Stereo-seq T FF V1.2 kit. scRNA-seq: Epidermal and dermal single-cell suspensions were mixed 1:1 and processed for 10x Genomics scRNA-seq 3' V3 Kit to generate scRNA-seq libraries. Libraries were sequenced on an Illumina NovaSeq PE150. Stereo-seq: libraries from the Complete Genomics Stereo-seq T FF V1.2 kit were sequenced using a DNBSEQ-T7.