title: genome resequencing  of Oncomelania hupensis
design: Foot tissues were extracted, rinsed with PBS buffer, and rapidly frozen in liquid nitrogen. Genomic DNA was extracted from all samples using the DNeasy Blood & Tissue Kit (Qiagen). DNA concentration was measured with a Qubit 4.0 fluorometer. A total of 0.2 g of DNA per sample was used to construct sequencing libraries. Briefly, genomic DNA was fragmented to ~350 bp by sonication, end-polished, A-tailed, and ligated to Illumina adapters, followed by PCR amplification. PCR products were purified using the AMPure XP system (Beverly, USA). The quality of the libraries was assessed on an Agilent 5400 system (Agilent, USA) and quantified using QPCR at 1.5 nM concentration. Libraries were pooled and sequenced on the Illumina NovaSeq 6000 platform (PE150 strategy) by Novogene Bioinformatics Technology Co., Ltd. (Beijing, China) based on library concentration and required data output.