title: GSM9162387: WT brain, 11months, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNAs were isolated using TRIzol  per the manufacturer's instructions. RNA was quantified using Nanodrop and stored at −80°C until shipped to RNA Sequencing Total RNA libraries were prepared with the Pico Input SMARTer Stranded Total RNA-seq Kit (Takara). Briefly, 10 ng of RNA from each sample was reverse transcribed using random priming and reverse transcriptase. Full-length cDNA was obtained with SMART (Switching Mechanism At 5′end of RNA Template) technology, which preserves the strand orientation of the RNAs. Ribosomal cDNAs were hybridized to mammalian-specific R-Probes and cleaved by ZapR. Libraries containing Illumina adapters with TruSeq HT indexes were then pooled and loaded onto the Hiseq 2500 platform. Single end reads at 75 base pair with 30 million reads per sample were generated for subsequent bioinformatic analysis.