title: CFS-treated-4hours
design: Methods: P. aeruginosa PA01 was cultured in TSB at 37C, 150 rpm for 6h, harvested, washed with PBS, and adjusted to a 0.5 McFarland standard. For treatment, the bacterial suspension was mixed with 1/2 CFS from Pediococcus pentosaceus (final 1/4 CFS, 12.5% v/v), while MRS broth served as the control. Samples were incubated at 37C, 120 rpm for 4h. Transcriptomic profiling was performed using paired-end RNA sequencing to compare gene expression between untreated and CFS-treated P. aeruginosa PA01.