title: GSM9382264: WTC11 hiPSC, Passage 52, high purity hPSM-CM differentiation, D8, Well Replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: For both the multi-omic collection experiment and the RNA sequencing only collection, RNA was harvested from Trizol-collected samples by tri-phasic extraction followed by purification and concentration with Zymo Direct-zol MiniPrep Plus columns (including on-column DNAase treatment). 500ng of total RNA per sample was used for the construction of sequencing libraries. For the multi-omic collection experiment, mRNA libraries for RNA sequencing were constructed using the Illumina TruSeq Stranded mRNA kit and sequenced on an Illumina NovaSeq6000. For the RNA sequencing only collection experiment, total RNA libraries for RNA sequencing were constructed using the Takara SMARTer Stranded Total RNA Kit - HI Mammalian and sequenced on an Illumina NovaSeq 6000.