title: RNA-Seq of Bixa orellena: Flower
design: Samples were harvested for RNA extraction and flash-frozen in liquid nitrogen and stored at -20C. RNA was extracted using the RNEasy Plant Mini Kit (Qiagan), following the manufacturers recommendations. After quality control, RNA-Seq libraries were developed using the KAPA mRNA Hyper Prep., following the manufacturers recommendations. In-house 8-bp dual indexed adapters were used. Sequencing was carried out at the DNA Technologies and Expression Analysis Cores at the UC Davis Genome Center.