title: GSM8682831: EVs  from plasma of Dicer cKO mice; 8 w old;2; Mus musculus; miRNA-Seq
library_construction_protocol: The purification of total exosome-derived RNA was carried out by the exoRNeasy Maxi Kit (Qiagen). A total amount of 3 μg total RNA per sample was used as input material for the small RNA library according to protocol of TruSeq Small RNA Sample Prep Kits(Illumina, San Diego, USA). And then we performed the single-end sequencing (1x50bp) on an Illumina Hiseq2500 at the LC-BIO (Hangzhou, China) following the vendor's recommended protocol.