title: GSM9461729: BS2; Ostrinia furnacalis; RNA-Seq
library_construction_protocol: Total RNA was extracted from gut tissues, and RNA integrity was assessed prior to library construction. RNA sequencing libraries were prepared and sequenced on an Illumina HiSeq platform by Novogene Co., Ltd. (Beijing, China). Three biological replicates were included for each treatment. After quality control, library concentrations were measured using a Qubit 2.0 Fluorometer (hermo Fisher Scientific, Waltham, MA, USA), and samples were diluted to 1.5 ng/μL. Insert sizes were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), and effective library concentrations were accurately quantified by qRT-PCR. Libraries were pooled according to effective concentration and target sequencing depth and subsequently subjected to Illumina sequencing.