title: PCR
design: Sequencing of target capture data from genomic DNA from tissue, library preparation through enzymatic shearing with the NEBnext Ultra II FS kit, targeting 300bp insert size, capture reactions using MyBaits-II kit (Arbor Biosciences, MI, USA; Ref# 170210-32) and protocol described in Wielstra et al. 'Phylogenomics of the adaptive radiation of Triturus newts supports gradual ecological niche expansion towards an incrementally aquatic lifestyle' (2019) - https://doi.org/10.1016/j.ympev.2018.12.032.