title: Illumina MiSeq paired end sequencing
design: Libraries for sequencing of the fungal ITS2 region were prepared according to Illumina�s �16S Metagenomic Sequencing Library Preparation� guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (�amplicon PCR�). The template specific primers were (without the overhang adapter sequence): ITS9F (5�- GAACGCAGCRAAIIGYGA-3�) and ITS4R (5�- TCCTCCGCTTATTGATATGC-3�).