title: GSM9056739: A431 cells shp53, WWOX Wild-Type, Input DNA; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde (methanol free, Thermo Scientific 28906) for 10 min at room temperature and quenched with glycine, 125 mM final concentration.Cells were sonicated using bioruptor sonicator to produce chromatin fragments of ~200–300 bp. The chromatin was immunoprecipitated by incubation with 5 μl of anti- ΔNp63 antibody (Rb polyclonal anti- ΔNp63 antibody (ab203826)). Immune complexes were captured with protein G Dynabeads. DNA was precipitated by phenol/chloroform/isoamylalcohol extraction. The ChIPed and the Input DNA were used to prepare libraries by Kappa Hyperprep kit