title: Whole exome sequencing derived from NSCLC Organoid
design: Genomic DNA was extracted and randomly fragmented into sizes of approximately 180280 bp.  T4 DNA ligase was then employed to ligate Illumina sequencing adapters to both ends of the fragmented DNA. The adapter-ligated library was hybridized in solution to a biotin-labeled probe library, enabling the capture of exonic regions. Sequencing was conducted on the NovaSeq-PE150 system.