title: GSM9533765: H2A-TerminaTOR cells, cycloheximide and PDGF treatment, replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNA extraction kit (Zymol).1 µg of total RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.