title: GSM8994799: Low passage, non-shipped, not irradiated HDF sample 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA extraction was done using 1mL TRIZOL as described above, with the addition of an on-column DNase digestion as described by the from Invitrogen PureLink RNA Mini Kit's instructions and was eluted in ultra-pure sterile water (Invitrogen). Library preparation and sequencing was performed at the University of Minnesota Genomics Center. Unique dual indexed paired-end TruSeq stranded libraries were created and pooled following rRNA depletion using the Ribo-Zero Plus rRNA Depletion kit. All libraries were gel size selected (≈ 200bp) and sequenced using an Illumina NovaSeq X Lane with a 2 x 150bp flow cell. Libraries were sequenced to a mean depth of ≥20M reads.