title: GSM9053712: KD_3´IMP1_LPS_PeMPs_3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Microglia were seeded on top of a 1 μm-pore membrane of a transwell system. Cells  were transfected with a control siRNA or an Imp1-targeting siRNA, and the lower compartment was then treated with vehicle or LPS. After removing the cell material  from the upper compartment with a cotton swab (see publication for details), total RNA from PeMPs was isolated using the Direct-zol RNA Miniprep kit following manufacturer instructions (ZYMO Research, Irvine, California, USA). RNA quality was addressed in a Bioanalyzer electrophoresis system (Agilent Technologies, Santa Clara, California, 1075 USA) After removing rRNAs, 2 ng of RNA was used to prepare total RNA libraries with the SMARTer Stranded Total RNA-Seq Kit (v3, Takara Bio, Kusatsu, Japan).  Sequencing was performed in a NovaSeq 6000 instrument (paired-end, 2x 100 bp. Illumina, San Diego, California, USA)