title: Talaromyces marneffei S50
design: The liquid culture solution of T. marneffei was subjected to ultrasonic dispersion. Genomic DNA was extracted from each isolate using a rapid fungi genomic DNA isolation kit (Sangon Biotech, Shanghai, China). After undergoing quality control, the DNA was randomly fragmented using a Covaris ultrasonic crusher. The resulting library was prepared through performing terminal repair, adding A-tials, adding sequencing linkers, and purifying. The library was then preliminarily quantified using Qubit 2.0, and the inserted fragments were analyzed using Agilent 2100 and q-PCR. Based to the desired concentration and target data, different libraries were prepared and loaded onto the flowcell. The cBOT was used to cluster the libraries, which were then sequenced using the NovaSeq6000 platform (Illumina, USA).