title: GSM9137177: 4DNEX5YGEMN1, Homo sapiens - U2OS, input-condition3enhancementG - anti-THRAP3, B2 T1; Homo sapiens; OTHER
library_construction_protocol: Cell were fixed with 1.6% paraformaldehyde for 20 minutes. After quenching, TSA-reaction (proximity-labelling) and cell lysis, genomic DNA was sonicated using Biorupter Pico sonicator (Diagenode). TSA-Seq libraries were constructed using TruSeq ChIP Library Preparation Kit (Illumina). 3-10 ng DNA was used as the starting material for input and ip samples. Libraries were amplified for 8 cycles  in a thermocycler. Post amplification, libraries were purified using  Beckman Coulter AMPure XP beads. Concentrations of individual libraries were estimated by Qubit high sensitivity DNA kit and also by qPCR and then pooled accordingly. Pooled libraries were sequenced from one end of the fragments on a NovaSeq X Plus with V1.0 sequencing kits.  Fastq files were generated and demultiplexed with the bcl-convert v4.1.7 Conversion Software (Illumina).