title: RNA-seq of Clogmia albipunctata: cal-zld RNAi, LNC14
design: This embryo was treated by cal-zld RNAi. Two library preperations were made from this embryo. At the desired stage the embryo was homogenized in lysis buffer and a centrifugation step was performed to pellet the nuclei. The nuclei were used for ATAC-seq library preperation and the supernatant was used to isolate RNA for RNA-seq library preperation.RNA was isolated from the ATAC prep by Zymo kit R2030. The RNA-seq library was prepared by the Genomics Facility at the University of Chicago. cDNA was synthesized using Takara Smarter v4 kit following the Takara Ultra-Low Input protocol. Library preperation by Illumina Nextera XT following the manufacturers protocol.Single embryo ATAC-seq library preparations were done essential as described in Drosophila melanogaster (Blythe and Wieschaus 2016; Soluri et al. 2020).This entry is the RNA-seq library.