title: GSM9428829: 1dpf-C-6-4; Danio rerio; RNA-Seq
library_construction_protocol: Total RNA was isolated using Qiagen Rneasy isolation kit. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification using New England Biolabs kit.