title: Amplification of AIV: Water sample from Cerea
design: 8 l RNA were reverse transcribed using the LunaScript  RT SuperMix Kit (New England Biolabs) and the HA gene was amplified with a multiplex PCR using two primer pools targeting clade 2.3.4.4b viruses (available upon request) and the Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs). Amplicons were sequenced on an Illumina MiSeqTM in 150 paired-end mode.