title: Head RNA-seq replicate 3: Drosophila mojavensis wrigleyi (subspecies)
design: The RNA extraction was assessed with the RNeasy Plus Kit for 10 female heads (10 days old). The library preparation was performed using stranded mRNA-seq with standard quantity (ligation), and 100 bp paired-end reads were generated on an Illumina HiSeq 4000. Illumina adaptors and low-quality reads were removed for downstream analysis with Trimommatic v.0.39, parameters: LEADING:3; TRAILING:3; SLIDINGWINDOW:4:15; HEADCROP:10; CROP:90.