title: amplicon sequencing of the fungal ITS2 region: low_salinity_root
design: Amplicon sequencing analysis by targeting the ITS2 region for fungi (amplified using the ITS7fITS4r primer pair). A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the reverse primer that served as a barcode to identify sequencing products in multiplexed libraries. Amplicons were sequenced via MiSeq Illumina v3 2300 bp paired-end reads (Greek Genome Center, Biomedical Research Foundation Academy of Athens (BRFAA)).