title: GSM9206999: live CD8+CD90.1+P1-3 transgenic TCRTAG CD8 T cells isolated 2d post activation in LMTAG infected spleens, rep 3; Mus musculus; ATAC-seq
library_construction_protocol: CD8 T cells were FACS-sorted into FCS containing tubes and after sort 10% DMSO were added, mixed and stored at -80°C until all samples were collected. Profiling of Chromatin was performed by ATAC-seq. Cells were thawed and washed in cold PBS and lysed. After cell lysis, transposition happened at 42ºC for 45 min. After purification of the DNA with the MinElute PCR purification kit (Qiagen), material was amplified for 5 cycles. Additional PCR cycles were evaluated by real time PCR. Final product was cleaned by Ampure Beads at a 1.5x ratio.