title: GSM8941218: PTBP1-immunoprecipitated RNAseq Primar culture Sertoli cell_3; Mus musculus; RIP-Seq
library_construction_protocol: NucleoSpin RNA plus (TaKaRa). For RNAseq of FACSed Sertoli cells, Poly-A RNA was isolated from 100 ng of total purified RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490), and converted into a sequencing library using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7420). For PTBP1-immunoprecipitated RNAseq, RNA-seq libraries from total RNA were generated using NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB).