title: L. serriola US96UC23 HiFi reads
design: Lactuca serriola (US96UC23) seedlings were grown in Magenta boxes under sterile conditions in the dark. Seven-day-old etiolated seedlings were harvested and flash frozen in Liquid N2. Etiolated tissue was used for DNA extraction using a CTAB DNA extraction/Sorbitol cleanup protocol. The HiFi library was prepared using PacBio SMRTbell prep kit 3.0 followed by bead clean up and size selection (&gt;13.6kb)