title: Metagenome of LLD4
design: The PacBio SMRTbell library was prepared for sequencing from the concatenated amplicons following manufacturers protocol.  In this protocol, DNA fragments were treated with DNA damage repair mix, end-repaired, and 5 phosphorylated. PacBio hairpin adapters were ligated to the fragments to create SMRTbell templates for sequencing. The SMRTbell templates were purified using exonuclease treatments and size-selected using the Sage Science BluePippin instrument with a 10 kb lower cutoff depending on DNA quality