title: GSM9164564: left brain, Day 0, open-field, fish 40E8; Gasterosteus aculeatus; RNA-Seq
library_construction_protocol: Each subject was killed by rapid cervical dissection and the brain was immediately dissected under a microscope in a Sylgaard 184-lined petri-dish. Half of the brain was placed in RNAlater, stored at 4C overnight, and transferred to -20C until RNA extraction. We extracted RNA using Macherey-Nagel NucleoSpin 96 RNA kits with an Eppendorf EpMotion 5075v liquid-handling robot. 242 samples (1.5 μg of RNA in 37.5 μl of RNase-free water) were sent to the Genomic Sequencing and Analysis Facility (GSAF) at the University of Texas at Austin for 3'Tag-seq library preparation and sequencing (6 lanes of Illumina HiSeq 2500, 100 nt reads).