title: Transcriptomic profiling of BrO, DMGO and GD2 CAR T cells
design: Transcriptomic profiling of BrO, DMGO and GD2 CAR T cells
library_construction_protocol: DMGOs treated with GD2 CAR T cells were dissociated 14 or 35 days after initial T cell addition with the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat. #130-092-628), as described above for preparation of single cell RNA and tracker seq libraries. Dissociated cells were washed and stained in FC buffer with CD3-APC (1:80; BD Biosciences, clone SK7) and LIVE/DEAD Fixable Near-IR Dead Cell Stain (1:1000; Thermo Fisher) for 30 min at 4ËšC. CD3+ T cells and GFP+ tumour cells were sorted on a CytoFLEX SRT Benchtop Cell Sorter (Beckman Coulter) and immediately processed for scRNA-seq.   Library generation was provided by Leiden Genome Technology Center (Leiden, The Netherlands)