title: Raw isoSeq reads from pleural ganglia on the side of long-term sensitization training, 1 day after training
design: 300ng of total RNA were converted to cDNA with the Iso-Seq Express Oligo Kit. cDNAs were converted into a library with the SMRTBell Prep kit 3.0 (Pacific Biosciences) Each library was sequenced on 1 SMRTcell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 30hs movie time.