title: GSM8859716: MT946_NCC d5 NOG rep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, T2010). RNA libraries were constructed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7760). 300 ng of total RNA input was used for each library. Libraries were sequenced on Illumina NovaSeq X Plus, producing 150bp paired-end reads. Barcoded libraries were made with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645L) using NEBNext Multiplex Oligos Dual Index Primers for Illumina (NEB, E7600S), and sequenced on Illumina NovaSeq X Plus, producing 150 bp paired-end reads. ATAC-seq was performed using 100,000 cells per sample. Libraries were prepared using the ATAC-seq Kit (Active Motif, 53150) and sequenced on Illumina NovaSeq X Plus producing 150 bp paired-end reads.