title: RNAseq, gill, Etroplus suratensis, 15 ppt, 3 hours, replicate 2
design: Total RNA was isolated using Trizol reagent and purified with Nucleospin RNA clean-up kit (Macherey-Nagel, Germany). The RNA quantity was estimated with Nanodrop 2000 (ThermoFisher Scientific, Massachusetts, USA) and the RNA integrity was checked with Agilent 2100 bioanalyzer (Agilent Technologies, California, USA). The cDNA library was prepared with KAPA Hyper Prep kit (Roche, Basel, Switzerland) and sequenced on Illumina Novaseq6000 platform to generate 2 X 150 bp paired-end RNAseq reads.