title: GSM8630784: TEP Stage_IFNA_Exp_2_Rep_2; Homo sapiens; RNA-Seq
library_construction_protocol: After the TEP stage, cells were dissociated into a single-cell suspension using Accutase (AT104) for 2–3 cycles of 5-minute incubations. Subsequently, cells were stained with EpCAM-APC, and the EpCAM-high population was sorted using a BD FACS Aria II SORP. Cell viability was assessed using 7-AAD staining. Total RNA was extracted from the sorted cells using the Qiagen RNeasy Kit. Illumina Stranded mRNA Prep 50-200ng Total RNA input were used to construct libraries followed by pooling libraries into NovaSeq6000 lanes at 2 x 100 bp read length.