title: GSM9462482: ICC CAF cells, shSLC2A1, Hypoxic condition, 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Fast Pure Cell / Tissue Total RNA Isolation Kit (Vazyme). The minimum total amount of RNA used for constructing the sequencing library was 3.24 μg Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.