title: sgRNA_amplicons_from_Mycobacterium_tuberculosis_H37Rv_Screen_I_5Generation_Post1st_Rec_withoutATc_rep3_of_3
design: sgRNA guide sequences PCR amplified from genomic DNA preparation of passaging experiment after 5 generations and post incubation in 1st stage minus Atc for 1 week with recovery in complete 7H9 media