title: GSM9023336: HPAEC, pressure group, 25 mmHg pressure, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: mRNA was isolated by pooling cells from 3 Luer I slides for each condition using the RNAeasy mini kit (Qiagen), quantified using Nanodrop and evaluated for quality with a bioanalyzer by the UTSW Genomics Core. All samples had a RIN score of 10. 1.5 µg of RNA was used for library construction. Illumina TruSeq® Stranded mRNA Library Preparation Kit (Catalog #20020594) was used for RNA-seq library construction.