title: Spatial transcriptomics and proteomics of post-transplant HCC recurrence using 10x Visium CytAssist
design: Spatial transcriptomics and proteomics of post-transplant HCC recurrence using 10x Visium CytAssist
library_construction_protocol: Human liver tumor samples were collected during surgical resection (primary and recurrent lesions). Sections (5 µm) were mounted onto 10x Genomics Visium slides (11 mm) using CytAssist, H&E stained, imaged, and stored at −80 °C. De-identified metadata (age, sex) were retained. No bulk extraction was performed. Poly-A RNA was captured in situ according to the 10x Genomics Visium manufacturer's instructions. For protein, oligo-conjugated antibodies were applied following the CytAssist Spatial Protein procedure, and excess reagents were removed by the recommended washes. Visium gene expression (GEX) libraries and Feature Barcode antibody-derived tag (ADT) libraries were prepared with 10x Genomics kits following the manufacturer's protocols. Libraries were quantified (Qubit) and quality-checked (Bioanalyzer/Tapestation) before pooling.