title: GSM9137104: Whole trancriptome; Gallus gallus; RNA-Seq
library_construction_protocol: For single-cell dissociation, the dissected tissue was briefly rinsed in cold HBSS and incubated in Accutase (Gibco) for 15 min at 37°C followed by mechanical dissociation. The cell suspension was analyzed with a hemocytometer to assess the quality of the dissociation and evaluate cell density. Dissociated cells were centrifuged at 350 g for 5 min at 4°C and resuspended in 0.25% BSA in PBS. We adapt this protocol with the help of Rhapsody Team to replace “AbSeq” methodology by LMOs identification. We performed a first PCR on the supernatant of BD Rhaspody cell capture beads. The total data depth was of 600GB  for WTA72h1207 and of 12GB for LMO72h1207.