title: RNA-Seq of mus musculus: adult male colon
design: messenger RNA was isolated according to polyA selection method by oligo(dT) beads and then fragmented by fragmentation buffer firstly, double-stranded cDNA was synthesized, the synthesized cDNA was subjected to end-repair, phosphorylation and adapter addition.Libraries were size selected for cDNA target fragments of 300 bp, followed by PCR amplified  for 15 PCR cycles. After quantified by Qubit 4.0, the sequencing library was performed on NovaSeq X Plus platform.