title: 16SRNA-seq of Onggole: in vitro sample
design: Sequencing of the 16S rRNA gene was performed at NovogeneAIT Genomics (located in Biopolis, Queenstown District, Singapore) using the Illumina MiSeq platform, a high-throughput sequencing system. Primer and barcode trimming, sequence quality control, alpha diversity analysis, and taxonomic assignment were conducted using QIIME2