title: GSM9161649: OVD_10_control; Mus musculus; RNA-Seq
library_construction_protocol: Plug-positive females were euthanized by cervical dislocation and tissue was dissected. Unpaired control females were euthanized and dissected in the morning, when determined to be in estrus. For each female the FRT was dissected, collecting tissue from both uterine horns (Us), both uterotubal junctions (UTJs) using precise microdissection and both oviducts (OVDs) (Fig. 1). For each female one of each U, UTJ and OVD was placed in RNAlater for RNA extraction. RNA was extracted from tissues stored in RNAlater using the RNeasy Plus Mini (uterine horn samples) or the RNeasy Plus Micro (for UTJ and OVD) kits (Qiagen) following the manufacturers recommendations. Samples were stored at -80º until sequencing. Samples were sequenced on a Illumina NovaSeq X platform, producing ~30 M, 150bp, paired-end reads per sample. Sample quality control and library preparation was performed by the sequencing facility (Novogene Europe). RNA integrity ranged from 6.3 to 9.8.