title: RNAseq of Mustela putorius furo: Adult female brain, survivor
design: RNA extracted from the brains of eight ferrets from the second challenge experiment were subjected to RNA sequencing. An aliquot of diluted ERCC ExFold RNA Spike-Mix 1 was added to the RNA, per manufacturer's guidelines, and the RNA was DNase-treated using ezDNase enzyme. RNA-seq libraries were prepared from 100, 500, or 1000 ng of the DNase-treated RNA using the TruSeq Stranded mRNA Library Prep Kit following a slightly modified manufacturers protocol, where Superscript II was replaced with Superscript III reverse transcriptase to generate cDNA. Libraries were sequenced on a NextSeq 2000 using the NextSeq 2000 P3 Reagent Kit (100-cycle) for 1 x 100 bp reads. The reads were aligned to the ENSEMBL ferret genome using HiSAT.