title: Ber_RNA-seq-root
design: Total RNA was extracted using the R6827 Plant RNA Kit (Omega Bio-Tek, Norcross, GA, USA) and RNA samples were examined using  NanoDrop One spectrophotometer (NanoDrop Technologies, DE) and Qubit 3.0 Fluorometer (Life Technologies, Carlsbad, CA, USA). mRNA was extracted from total RNA, and the library was constructed through a series of steps including enrichment, cutting, cDNA synthesis, PCR amplification, and cyclization