title: GSM9349803: 241018_SSc_FB_siRNA_RUNX1_12; Homo sapiens; RNA-Seq
library_construction_protocol: Cell pellet was resuspended in 1X PBS+0.04% BSA, and cell viability was assessed with 0.4% Trypan Blue. Cells were fixed and permeabilized with 10X Genomics fix/perm buffer for 16 hours at 4°C, followed by incubation with quenching and enhancer buffer on ice, as per the instructions in Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (10X Genomics, catalog number PN-1000414). Samples were stored in 10% glycerol until sequencing. Sequencing was performed at the University of Michigan Advanced Genomics Core.