title: RNA-Seq of T. indictiva from male gonad
design: RNA template were isolated with AllPrep DNA/RNA Micro Kit (Qiagen) following manufacturers protocol. The quality of RNA was checked with Agilent 2100 Bioanalyzer (Agilent Technologies) and the sample concentration was measured using Qubit RNA High Sensitivity Assay Kit (Thermo Fisher Scientific). Construction of the cDNA libraries and sequencing of the 150bp long PE reads on Illumina NovaSeq 6000 were carried out by Novogene (United Kingdom)