title: GSM9347816: SCR_R2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using an Rneasy mini plus kit (Qiagen). RNAseq-PolyA technique was applied by NuCore aeq facility. Briefly, PolyA+RNA was captured and then fragmented and primed for cDNA synthesis. Following cDNA synthesis, 3' ends were adenylated and 5' ends repaired. DNA sequencing adapters were ligated and the ligated fragments were PCR amplified.