title: WGS of Vibrio agarivorans isolated from Sargassum fluitans
design: DNA sample quality assessment, DNA library preparation, and sequencing were conducted at Azenta Life Sciences (South Plainfield, NJ, USA). DNA clean-up was performed using a 0.8x AMPure XP bead cleanup protocol (Beckman Coulter, CA, USA). Genomic DNA was quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), and integrity was assessed using the TapeStation (Agilent Technologies, Palo Alto, CA, USA). The sample was then prepared using the Native Barcoding Sequencing Kit V14 (Oxford Nanopore Technologies) according to the manufacturers protocol. Briefly, genomic DNA fragments were end-repaired, barcoded adaptors were ligated, the mixture was pooled, the motor protein was added, and the mixture was loaded onto a PromethION flow cell. The libraries were run with live SUP base-calling with demultiplexing using MinKNOW to generate individual fastq.gz files.