title: K_CK_1
design: Root samples from each treatment were frozen in dry ice and sent to Major Biomedical Technology Co., Ltd. (Shanghai, China) for RNA sequencing using Illumina Novaseq 6000 and library preparation with the Illumina TruseqTM RNA sample prep Kit. Three biological replicates were set for each treatment. After quality control using Fastp, the sequences were aligned to the Nicotiana_tabacum reference genome (Ntab-K326, https://solgenomics.net/organism/ Nicotiana_tabacum/genome) using HISAT2.