title: GSM8457546: As3mtd2d3_tg, E15.5, replicate2, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: For E15, freshly collected isocortex from AS3MTd2d3-Tg (mCherry) or littermate (no signal) embryos were flushed with ice-cold HBSS (Mg2+/Ca2+ free) to remove debris. Single cell isolation was performed by incubation of tissue with digestion buffer (0.1% DNase I + 0.25% Trypsin) in 37°C  incubator for 10 min. Added neurobasal media with 10mM HEPES and passed through the Pasteur pipette several times. Stored in ice before the library generation step. For P60, freshly collected isocortex from AS3MTd2d3-Tg (mCherry) or littermate (no signal) mice were flushed with ice-cold HBSS (Mg2+/Ca2+ free) to remove debris. Transferred the tissues to MACS® Tissue Storage Solution in ice before the automated nuclei isolation was performed by Singulater 100. For E15.5 scRNA-seq and P60 snRNA-seq, we used 'Chromium Single Cell 3p RNA library v3' kit. Library construction was performed following 'Chromium Single Cell 3' Reagent Kit User Guide v3'.