title: GSM9056397: WTCD452_MZ_M1; Mus musculus; RNA-Seq
library_construction_protocol: Cell pellets were lysed after FACS in Buffer RLT (QIAGEN) with 1:100 beta-mercaptoethanol then frozen until processing. Lysates were homogenised using QIAshredder spin columns then RNA was extracted using the RNeasy Mini or Micro kit (QIAGEN) with DNase digestion on a QIAcube Connect, all according to the manufacturer's instructions. cDNA libraries were generated using the Watchmaker RNA Library Prep Kit with Polaris Depletion of rRNA and globin transcripts according to the manufacturer's instructions.