title: Amplicon_seq_rumen_fluid_ 41706 EX D70
design: Beef bulls were offered different diets that had potential methane mitigating properties. Rumen fluid was collected from the bulls at 3 timepoints; day 0 (pre supplementation), d 35 (mid supplementation), d 70 (end of supplementation). Rumen fluid was snap frozen in liquid nitrogen and stored at -80 C. Rumen fluid was thawed and DNA was extracted using the DNeasy PowerSoil Pro Kit (QIAGEN GmbH, Qiagen Strasse 1, 40724 Hilden, Germany) . DNA was sent to Edinburgh Genetics for amplification, library preparation and amplicon sequencing of bacteria, protozoa, archaea and fungi.