title: 16S rRNA of mangrove sediment microbiome
design: The total DNA of 0.20  0.04 g of sediment was extracted using the DNeasy PowerSoil Pro extraction kit (QIAGEN, Hilden, Germany) following the manufacturers instructions. The extract quality was confirmed via gel electrophoresis, and the concentration was determined using nanodrop (NanoDrop 2000, Thermo Fisher Scientific, Waltham, Massachusetts, USA), and subsequently, each sample was standardised to 2.5 ng/L. The V4-V5 of the 16S rRNA gene was amplified using the universal earth microbiome project primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5CCGYCAATTYMTTTRAGTTT-3)56. One-step PCR of the target amplicon was performed using Nextera XT v2 index primers. Each sample was amplified in triplicate with each reaction containing 5 L of total DNA in a final reaction volume of 20 L.