title: sequences of bacteria
design: After PCR amplification and purification of the target fragment, a sequencing library was established using Illumina's TruSeq Nano DNA LT Library Prep Kit. Double ended sequencing of community DNA fragments using MiSeq Reagent Kit V3 reagent through Illumina MiSeq platform.  The raw data obtained from high-throughput sequencing was subjected to sequence denoising and clustering using QIIME2's dada2 analysis process, and the obtained ASVs were annotated using the Greengenes database.