title: RNA-seq of tumor tissue from metastatic colorectal cancer patient
design: Total RNA was extracted using the RNeasy Mini Kit (Qiagen). RNA-seq libraries were prepared with the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara Bio) using rRNA depletion and sequenced on Illumina NovaSeq X Plus (paired-end 150 bp).