title: 16S rRNA amplicon sequencing of Homo sapiens: oral tissue samples from  oral squamous cell carcinoma patients6
design: The 16S rRNA gene amplicon sequencing libraries were constructed as follows: Genomic DNA was used as a template for PCR amplification using barcoded universal primers and Premix Taq (TaKaRa). The resulting PCR products were quantified, pooled in equimolar amounts, and purified using the E.Z.N.A. Gel Extraction Kit. The final sequencing library was prepared from the purified amplicon pool using the NEBNext Ultra DNA Library Prep Kit for Illumina, following the manufacturer's standard protocol.