title: GSM9429435: daf-16(lf); daf-2(lf), all cells, rep 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: RNA was extracted from sorted cells using TRIzol LS followed by chloroform and cleaned up using Zymo RNA Clean & Concentrator-5. For single-end samples, RNA was assessed by Fragment Analyzer, and high quality total RNA was subjected to library preparation using the Illumina TruSeq Stranded mRNA Library Preparation Kit (Cat# 20020594) according to the manufacturer's instructions. For paired-end samples, RNA quality was assessed by Agilent Bioanalyzer 2100. The full length cDNA library was first generated from high quality total RNA using TaKaRa SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat# 634890) according to manufacturer protocol. The final barcoded sequencing libraries were prepared from the full length cDNA using Illumina Nextera Flex according to manufacturer protocol.