title: GSM9566785: Wollemia pollen sRNA replicate2; Wollemia nobilis; ncRNA-Seq
library_construction_protocol: Total RNA from leaf (4 technical replicates), mature pollen (3 technical replicates) and, ovules (3 technical replicates) was isolated using CTAB buffer (2% CTAB, 2% PVP-K30, 100 mM Tris-HCl pH 8.0, 25 mM EDTA, 2 mM NaCl, 0.5 g/L spermidine, 2% ß-mercaptoethanol). NEXTFLEX Small RNA-seq kit v3 (Perkin Elmer) was used according to the manufacturer's instructions to prepare the small RNA libraries.