title: GSM9162436: K2; Homo sapiens; OTHER
library_construction_protocol: Tissues were surgically excised, embedded in optimal cutting temperature (OCT) compound (SAKURA), rapidly frozen on dry ice, and stored at −80 °C until cryosectioning. Samples with an RNA integrity number (RIN) ≥ 7 were sectioned at 10 μm thickness using a cryostat (Leica CM1950) and subsequently subjected to fixation, staining, imaging, pre-permeabilization, and permeabilization. After cDNA transfer from the slides, barcoded cDNA was purified, amplified, fragmented, A-tailed, adaptor-ligated, and index PCR–amplified. Final libraries were quantified using the Qubit High Sensitivity DNA assay (Thermo Fisher Scientific) and assessed for size distribution with a High Sensitivity DNA chip on a Bioanalyzer 2200 (Agilent). All libraries were sequenced by illumina sequencer (Illumina, San Diego, CA) on a 150 bp paired-end run.