title: K562 cells treated with 10ng/ml TNFα, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags
design: K562 cells treated with 10ng/ml TNFα, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags
library_construction_protocol: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification. 