title: Transcriptomic assessment of parental and radioresistant human cell line models of prostate cancer
design: Transcriptomic assessment of parental and radioresistant human cell line models of prostate cancer
library_construction_protocol: For each sample, pellets containing up to 10^6 cells were collected by trypsinisation and centrifugation, followed by snap-freezing on dry ice. Bulk RNA was extracted from pellets using Qiagen AllPrep DNA/RNA Mini kit (Cat. No. 80204), according to the manufacturer's instructions. Individual libraries were generated from 500ng total RNA using Lexogen's QuantSeq 3' mRNA-Seq V2 Library Prep Kit (FWD) for Illumina with UDI 12nt, according to the manufacturer's protocols. 