title: GSM9381110: healthy donor 2 bulk RNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: For bulk RNA-sequencing, PBMCs from renal cell carcinoma and healthy patients were isolated and prepared as described above. Once resuspended, 150,000 cells were aliquoted. From these cells, RNA was extracted using the Invitrogen RNAqueous-Micro Total RNA Isolation Kit (#AM1931), and reverse transcription and tagmentation were conducted according to Picelli et al. using the SmartSeq2 protocol. For bulk RNA-sequencing, after cDNA synthesis and tagmentation, the library was amplified with the Nextera XT DNA Sample Preparation Kit (#FC-131-1096) according to protocol. Samples were then sequenced on one lane of the Illumina Hiseq4000 with paired end 100-bp read length.