title: Oxford Nanopore sequencing of 5hmC-modified synthetic sustech seqs DNA
design: The cytosine in the library is fully substituted by 5hmC during PCR amplification. Library preparation was performed using the Oxford Nanopore Technologies (ONT) Ligation Sequencing Kit (SQK-LSK110) following the manufacturers protocol. The sequencing was carried out on a Nanopore MinION device. Reads are basecalled with reinforced 5hmC DeepSME basecaller at https://github.com/sparkcyf/DeepSME and could be decoded to binary data via https://github.com/sparkcyf/DeepSME_DNA_Storage_Decode_scripts.