title: GSM9161334: Mega_Sublibrary1_CRISPR; Homo sapiens; OTHER
library_construction_protocol: Cells were fixed and stored at -80°C according to a proprietary protocol from Parse Biosciences (Evercode Cell Fixation v3) We used a combinatorial barcoding approach from Parse Biosciences (Seattle, USA). Samples were processed according to Parse Biosciences Manual (Evercode WT Mini and Mega v3 with CRISPR Detect), (sub-)libraries were prepared for transcriptomics and seperately for sgRNA detection, each library was tested on Agilents TapeStation before submitting for sequencing. Each library is pooled due to combinatorial barcoding, and cell identidies are determined during analysis using an individual sample processing sheet.