title: 16S rRNA gene amplicon sequence of broiler chicken caecal microbiome
design: The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using universal primers 341F and 805R. PRC amplifications were performed in triplicate for each sample, and the resulting products were pooled to minimise amplification bias. Sequencing was performed on an Illumina MiSeq platform using the MiSeq v3 reagent kit (2 _ 300 bp paired-end reads), with a PhiX control library included as an internal sequencing control, following the standard Illumina MiSeq System User Guide.