title: DNA-seq of Homo Sapiens
design: Blood plasma samples were centrifuged for 15 min at 1900 g and 4 C. The supernatants were collected and centrifuged for 10 min at 16,000 g and 4 C and then stored at 80 C. Circulating tumor DNA (ctDNA) from blood plasma was isolated using the QIAamp MinElute ccfDNA Midi Kit. Libraries were prepared using the Solo-test Driver panel (53.8 Kb, 474 amplicons) based on a multiplex PCR with two pools of primers. Amplicons were partially digested and ligated to universal adapters, purified on magnetic beads, and amplified with index primers. The index primers encode individual 8-base indices as well as an adaptor sequence, which can then bind to the flow cell through the adaptor sequence, and they can be identified through the sequencing of its unique index combination. Libraries were sequenced on a Genolab M (GeneMind, Changsha, China) and FASTASeq300 (GeneMind, Changsha, China) at a read length of 2  150 bp.