title: RNA-seq of mouse: AL, Weeek 80
design: Total RNA was isolated from the frozen MFP tissues stored at -80oC. Tissue samples were homogenized in Direct-zol (Zymo Research, Irvine, CA, USA) with a tissue homogenizer. After homogenization, the remaining tissue residual was removed from suspension and RNA isolation was performed with a Direct-zol RNA MiniPrep Kit according to the manufacturers instructions (Zymo Research, Irvine, CA, USA, Cat No: R2052). RNA concentrations were determined with a NanoDrop 2000 spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA) and total RNA integrity was checked by 1% agarose gel electrophoresis. RNA samples were sequenced using the Illumina Novaseq (Illumina, San Diego, CA, USA) sequencer at the Murdoch University Genomics Centre, Perth, WA, Australia, resulting in more than 50 million paired reads per sample on average.