title: RNA-seq of Drosophila melanogaster: Third-instar larval hemocytes
design: The RNA was isolated from approximately 200 larval hemocytes. The isolated total RNA was quantified using Nanodrop. The integrity of RNA was evaluated on 1% agarose gel. Raw reads were checked for base quality and adapter content using FastQC (v0.12.1). Fastp (0.23.2) was used to remove adapter content and trim low-quality bases. The reads were mapped to the Drosophila melanogaster BDGP6.46 reference genome using STAR aligner. The aligned genes were quantified using HTSeq. The genes showing differential expression between the RNAi samples and Control samples were analyzed using the R package DESeq