title: DNA-seq of Homo Sapiens
design: Tumor DNA was isolated from archival FFPE blocks using the ExtractDNA FFPE kit. Libraries were prepared using the Solo-test Driver panel (53.8 Kb, 474 amplicons) based on a multiplex PCR with two pools of primers. Amplicons were partially digested and ligated to universal adapters, purified on magnetic beads, and amplified with index primers. The index primers encode individual 8-base indices as well as an adaptor sequence, which can then bind to the flow cell through the adaptor sequence, and they can be identified through the sequencing of its unique index combination. Libraries were sequenced on a Genolab M (GeneMind, Changsha, China) and FASTASeq300 (GeneMind, Changsha, China) at a read length of 2  150 bp.