title: GSM9535618: Knockout, female mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted directly from the cell pellets using the Qiagen RNAeasy Micor Kit following manufacturer's protocols. Total RNA was eluted and was quality checked on an Agilent Fragment Analyzer and Qubit (Invitrogen). Total RNA (100 ng) was used to generate RNA libraries following the Illumina RiboZero Total Stranded RNA library prep kit with ribosomal RNA removal. Following RNA library preparation and quality control, final libraries were pooled to 10 nM and the concentration of the pool was determined using the QuantaBio Universal qPCR reaction kit. After final dilution and denaturing, the pooled library was loaded onto a NovaSeq6000 (PE100) for sequencing at 250 pM as final concentration.