title: Alcaligenes phage SRA-1 isolation and genomic characterization.
design: The phage genome extracted following Soleimani et al. (2021). Briefly, the phage suspension was treated with DNase I and RNase A (Sinaclon Co, Tehran, IRAN) to remove undesirable debris of bacterial nucleic acids. Then the phage was sedimented with pure acetone (pH: 5.5 adjusted by acetic acid) (v/v 1:4) and centrifuged (15000 g, 5 min). Next, the phage pellet was lysed by lysis buffer (Potassium-iodide 5M) and add to a silica-based spin column. It washed with washing buffer A (10 mM NaCl, 1 mM Tris-HCl pH 7.5 in ethanol 80 %), centrifuged (15000g, 1 Min) and then washed with washing buffer B (95 % ethanol), centrifuged (15000g, 1 Min) and dried at room temperature. Then, sediment of DNA on silica-base of column suspended in 80 l nuclease free water (DEPC treated water). The phage genome DNA was stored at - 20 C until sequencing. DNA libraries were prepared by DNA fragmentation, adapter ligation, and amplification, and then subjected to the whole-genome DNA sequencing with 2 150 bp paired end reads, carried out by the Novogen Company (United Kingdom) and sequenced by Illumina NovoSeq 6000.