title: GSM8653692: VICs, shVAPB-1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from cells using the FastPure Cell/Tissue Total RNA Isolation Kit (Vazyme, China). 1µg of total RNA was used for the construction of squencing libraries. After the total RNA samples were tested to be qualified, the MGIEasy RNA  library preparation kit was used for library construction.  Magnetic beads  with oligo (DT) were used for enrichment and purification;  Fragment  buffer was added to the purified mRNA to make its fragment into a short  fragment;  the fragment mRNA was used as a template for reverse  transcription into double stranded cDNA with six base random primers,  and then the product was purified.  The purified double stranded cDNA was  subjected to end repair, addition of dA tail, linker connection and PCR  amplification.  After the amplified product was qualified, it was denatured  and single stranded cyclized, and the cyclized product was inspected