title: 16S rRNA metagenomic sequencing of diseased shrimp: intestine
design: DNA was extracted from shrimp intestine using DNeasy PowerSoil Pro DNA Kit. The V3-V4 variable region of the bacterial 16S rRNA was amplified by PCR using 2X sparQ HiFi PCR master Mix. PCR product of approximately 550 bp was purified and indexed with forward and reverse Nextera XT index primer. Then, 16S rRNA amplicon of approximately 630 bp were cleaned, pooled, diluted, and loaded onto an Illumina MiSeq.