title: GSM9023249: C57BL/6_Mtb_HN878_d14_BAL_2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from lung homogenates using the DirectZol Mini Kit with on-column DNase I digestion (Zymo), as per manufacturer's instructions. BAL cell pellets were resuspended in TRI reagent and vortexed at high speed for ≥10 seconds to lyse. RNA was extracted from BAL lysates using the DirectZol Mini Kit with on-column DNase I digestion (Zymo), as per manufacturer's instructions. RNA integrity of all samples was determined to range from 5.7 – 9.4 (median 7.9) RNA-seq libraries from whole BAL and lung of HN878-infected C57BL/6 and C3HeB/FeJ mice and from lung tissue samples obtained from anti-IFNAR-treated HN878-infected C3HeB/FeJ mice were prepared using the total RNA library prep kit with Polaris ribosomal RNA depletion (Watchmaker Genomics)