title: Tn-seq of Pectobacterium aquaticum A212-S19-A16 after growth in TSB1/2 agar plate ; replicate 2
design: First, DNA was extracted from bacteria and digested with the enzyme MmeI, which cuts near the transposon insertion sites. The resulting DNA fragments containing the transposon and adjacent genomic DNA were isolated and tagged with unique barcoded adaptors. These adaptors contain sequences that allow for PCR amplification and sequencing.The transposon-adjacent DNA was then amplified by PCR, further purified, and checked for quality. Finally, the prepared DNA libraries was sequenced using high-throughput sequencing technology. This sequencing data can then be used to map the location of transposon insertions across the genome and study their effects.