title: Illumina MiSeq paired end sequencing
design: Libraries for sequencing were prepared according to Illumina�s �16S Metagenomic Sequencing Library Preparation� guide, with the exception of using Qiagen HotStar Taq DNA polymerase for the first PCR (�amplicon PCR�). The template specific primers were (without the overhang adapter sequence): 515F-5 (5�- GTGYCAGCMGCCGCGGTAA-3�) and 926R (5�- CCGYCAATTYMTTTRAGTTT-3�).