title: GSM9358853: MC38 tumor, Control, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were mechanically dissociated and enzymatically digested using the Mouse Tumor Dissociation Kit (Miltenyi Biotec) following the manufacturer's instructions. Spleens were collected, minced with a razor blade, and passed through a 70 μm cell strainer. Cells were washed in culture medium containing 10% fetal bovine serum (FBS; Gibco) and treated with ammonium chloride solution (STEMCELL) to lyse red blood cells (RBCs). Approximately 20,000 single cells per sample were loaded onto the 10× Chromium system for barcoding and cDNA synthesis, following the manufacturer's instructions. Quality control of cDNA and libraries was assessed using TapeStation before sequencing on the NovaSeq platform (Illumina), targeting a median read depth of 30,000 reads per cell.