title: WGS
design: ake the glycerol bacteria stored at -80, streak culture and activate in LB liquid medium to logarithmic phase, centrifuge 4mL bacterial solution at 4000g and 4 for 10min, discard the supernatant, wash the precipitate with sterile water 2-3 times to ensure the bacterial precipitate 2g, send 2-3 parallel samples, store at -80 and send for inspection with dry ice.