title: GSM8859096: Time 6 UVB1_B (rep3); Lactuca sativa; RNA-Seq
library_construction_protocol: Total RNA was isolated from the leaves (100mg) following a TRIZOL based method with modifications as previously described (Leonetti et al. 2021,  https://doi.org/10.1111/nph.16932). The quality of the RNA for each sample has been assured through Spectrophotometric evaluation (Nanodrop OneC, Thermo Fisher Scientific) and RNA integration Number (RIN) using Bio analyzer (Agilent Technologies Inc., USA), and qualified samples were proceeded to library construction. RNA amplification and cDNA library was prepared using the Nextera XT (Illumina, San Diego, CA, USA) by random fragmentation of the cDNA samples, followed by 5' and 3' adapter ligation. Each fragment was then amplified into distinct, clonal clusters through bridge amplification. The purified amplification products were sequenced on a Novaseq-PE 150 platform. RNA amplification and cDNA library was prepared using the Nextera XT (Illumina, San Diego, CA, USA) by random fragmentation of the cDNA samples, followed by 5' and 3' adapter ligation. Each fragment was then amplified into distinct, clonal clusters through bridge amplification. The purified amplification products were sequenced on a Novaseq-PE 150 platform.