title: GSM9679931: Sham-operated mouse brain, replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from ischemic hemispheres using Magzol Reagent (Magen) following the manufacturer's instructions. RNA quantity and quality were assessed using K5500 spectrophotometer (Kaiao) and Agilent 2200 TapeStation. Poly(A)+ mRNA was enriched using NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB), fragmented to ~200 bp, and processed with NEBNext® Ultra™ RNA Library Prep Kit for Illumina. Libraries were validated using Agilent 2200 TapeStation and Qubit (Thermo Fisher).