title: RNA-seq of Anopheles stephensi 5days old, sugar-fed, male central brain
design: Total RNA was extracted from ACME-fixed central brain samples, using Direct-zol RNA Microprep kit from Zymo research. 3 biological replicates each were prepared from mosquitoes at different physiological conditions of blood-hunger, blood-satiety or no-interest. cDNA library was prepared using NEBNext Ultra II Directional RNA Library Prep kit and paired-end sequencing was carried out on the Illumina Hiseq 2500 sequencing platform.