title: Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
design: Biomolecular Condensate Coronas from Nanoparticle Intracellular Processing Are Transferred Between Cells Allowing Cytosolic and Nuclear Access
library_construction_protocol: Cells were exposed to nanoparticle suspension for 10 min (pulse). The suspension was removed. Cells then were incubated complete medium containing FBS for 4 h (chase). After the chase, the supernatants were collected and clarified to remove cell debris. The exported particle complexes were pelleted by centrifugation at 12,000 x g for 40 min at 4 Â°C.  A549 cells were cultured in normal growth media. The cells were treated with nanoparticles for 10 min pulse followed by 4 hour chase. The conditioned media were collected. We used QIAGEN miRNeasy Micro Kit (Cat. No. 217084) to extract RNA. MGIEasy RNA Library Prep Set