title: bfr60_DMBA
design: Inguinal mammary glands were sampled from female Sprague-Dawley rats exposed to either 0, 0.06 or 60 mg of BFRs/kg of BW/Day during gestation and lactation. At weaning, dams were transferInguinal mammary glands were sampled from female Sprague-Dawley rats exposed to either 0, 0.06, or 60 mg of BFRs/kg of BW/Day during gestation and lactation. At weaning, dams were transferred to a normal diet, and mammary tumours were induced by DMBA by gavage (2mg/mL in corn oil) on the second day of involution.  Mammary glands were harvested 175 days after gavage and snap frozen. Tissues were reduced to powder in liquid nitrogen, and RNA was extracted using the Aurum total RNA fatty and fibrous tissue kit (Bio-Rad, Ontario, Canada, no. 7326830).red to a normal diet et mammary tumour where induced by DMBA by gavage (2mg/mL in corn oil) at the second day of involution.  Mammary glands were harvested 175 days after gavage and snap frozen. Tissues were reduced to powder in liquid nitrogen, and RNA were extracted using the Aurum total RNA fatty and fibrous tissue kit (Bio-Rad, Ontario, Canada, no. 7326830).