title: DNA-Seq of enoicyla pusilla: adult male specimen
design: A library was prepared with the  SMRTbell Express prep kit 3.0 (PacBio, Menlo Park, CA, USA) according to the instructions of Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 (PacBio, Version 01, April 2022). Total input DNA was approximately 820 ng . Quality control of the final library was again performed on the Quantus Fluorometer and the Agilent 4150 TapeStation system. Annealing of sequencing primers, binding of sequencing polymerase, and purification of polymerase-bound SMRTbell complexes were performed using the Revio polymerase kit (PacBio, Menlo Park, CA, USA). The loading concentration for sequencing was 260 pM. Long-read whole-genome sequencing was performed on a PacBio Revio instrument (PacBio, Menlo Park, CA, USA), 24 h HiFi sequencing.