title: GSM8631181: MEFs, P3, replicate 2, MeRIP; Mus musculus; OTHER
library_construction_protocol: IP group: RNA was extracted from MEFs and fragmented to ~150 nucleotides by RNA fragmentation reagents. Fragmented RNA was incubated with m6A primary antibody (Synaptic Systems, Germany) at 4°C for 2 h for immunoprecipitation. Eluted RNA was precipitated by 75% ethanol and treated with RNasin (Ambion, USA) according to the manufacturer's instructions.Input group: total RNA was extracted from MEFs and TruSeq Stranded mRNA Sample Pre-Kit (Illumina, USA) was utilized to prepare the library. TruSeq Stranded mRNA Sample Pre-Kit (Illumina, USA) was utilized to prepare the library from immunoprecipitated RNA.