title: RNA-seq of OE-44 callus
design: mRNA enrichment was performed on total RNA using oligo(dT)-attached magnetic beads. Theenriched mRNA with poly(A) tails was fragmented using a fragmentation buffer, followed by reversetranscription using random N6 primers to synthesize cDNA double strands. The synthesized doublestranded DNA was then end-repaired and 5'-phosphorylated, with a protruding 'A' at the 3' endforming a blunt end, followed by ligation of a bubble-shaped adapter with a protruding 'T' at the 3'end. The ligation products were PCR amplified using specific primers. The PCR products weredenatured to single strands, and then single-stranded circular DNA libraries were generated using abridged primer. The constructed libraries were quality-checked and sequenced after passing thequality control.