title: DNA-Seq of fish spoilage: muscle Day 1
design: The whole DNA present in the fish tissues was extracted and the 16S rRNA gene amplicon libraries were prepared using the ONT 16S barcoding kit. Barcoding of amplicons was performed using ONTs native barcode primers provided in the kit, and equimolar amounts of barcoded amplicons were pooled. The final pooled library was prepared by adding the sequencing adapters using the ONT ligation-based chemistry. Sequencing was performed on PromethION 2 Solo platform (Oxford Nanopore Technologies, Oxford, UK)