title: RNA-seq of the livers of HFD fed Activin E KO mice
design: RNA-seq of the livers of HFD fed Activin E KO mice
library_construction_protocol: Liver tissue was collected from Activin E KO mice (male). Samples were stored at -80°C until RNA extraction. Total RNA was extracted from liver tissue using Isogen II reagent (Nippon Gene, Tokyo, Japan) according to the manufacturer's protocol. mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module. The RNA-seq library was constructed with the NEBNext Ultra II Directional RNA Library Prep Kit following the manufacturer's instructions, including fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.