title: Illumina sequence of LR-PCR amplified mitochondrion of Dyopedos bispinis, voucher ZMMU WS19175
design: DNA was extracted using a QIAamp Fast DNA Tissue Kit. Eight pairs of primers for long-range PCR amplification of the mitochondrial genome were designed based on the reference genome. The Long-Range PCR products were generated using Phanta Max Super-Fidelity DNA  Polymerase (Vazyme, China). Then, the amplicons belonging to one sample were pooled proportionally to their total genomic concentration. Each pool was sheared on a Covaris S220 (Covaris, Woburn, MA, USA) to a target size of ~350400 bp. The libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit by New England Biolabs (NEB, MA, USA) and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 3) by NEB following manufacturer protocol. The constructed libraries were sequenced on an Illumina MiniSeq with a paired-end read length of 150 and NextSeq550 with a paired-end read length of 150.