title: Lake Rishateni water
design: The library kit for metagenome shotgun sequencing used in this study is TruSeq DNA PCR-Free Kit. Briefly, the sequencing library was prepared by using random fragmentation of the gDNAs followed by 5' and 3' ligation using P5 and P7 TruSeq DNA PCR-free kit adapters.  These adapters contain sequences for clustering and sequencing primers. Afterwards, the library quality and quantity were fluorometric assessed in order to measure library concentration and fragment size distribution. Lastly, the libraries were loaded into the sequencer; Illumina sequencing by synthesis technology was utilized via NovaSeq sequencer.