title: RNA-seq of Hedychium coronarium: flower
design: total RNA from four tissue samples was pooled and poly(A)+ mRNA was enriched using oligo-dT magnetic beads. Following RNA fragmentation, cDNA was synthesized using random hexamer primers. The library was constructed through a series of steps including end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification, followed by quality control assessment