title: Lates calcarifer,Muscle, Oxford Nanopore Reads
design: Genomic DNA was isolated from muscle tissue of a single fish using Phenol:chloroform method. DNA quality and quantity were assessed by Nanodrop 1000 spectrophotometer, Qubit 4.0 Fluorometer and agarose gel. Sequencing library was constructed using using Oxford Nanopore Native Barcoding Kit (Oxford Nanopore #96 v14) and assessed using Qubit 4.0 fluorometer and Agilent Tapestation . The libraries were size sequenced on PromethIon system.