title: RNA-Seq of Hibiscus mutabilis: leaf
design: High-quality genomic DNA was extracted from plant tissue using the CTAB method. Sequencing libraries were constructed using the MGIEasy enzymatic fragmentation DNA Library Prep Kit (MGI). The procedure included: random fragmentation of genomic DNA using Frag Enzyme II; size selection of target fragments with magnetic beads; end repair and A-tailing; ligation of MGIEasy DNA adapters; PCR amplification and purification; followed by denaturation, single-strand circularization, and enzymatic digestion to finalize the library. Paired-end sequencing was performed on the DNBSEQ-400 platform. Library preparation and sequencing were conducted by Xi'an Haorui Gene Technology Co., Ltd.