title: lcWGS of thrigmopoeus truculentus_tt17
design: Genomic DNA was extracted from the received spider leg tissue samples using commercially available QIAamp DNA Mini Kit (Qiagen) as per manufacturers instruction. The quality and quantity of the extracted DNA sample was checked on NanoDrop followed by agarose gel electrophoresis. The QC passed and confirmed DNA samples were processed for paired-end sequencing library using QIAseq FX DNA Library Preparation Kit as per manufacturers instruction. Briefly, approximately 100ng of DNA was processed for fragmented, end-repaired and adaptor ligation in a single tube as per the kit protocol. The end-repaired DNA was processed for size selection using AMPure XP bead. The size-selected products were PCR amplified with the index primer as described in the kit protocol. The PCR enriched libraries were analyzed on 4200 Tape Station system (Agilent Technologies) using D1000 Screen tape as per manufacturer instructions. After obtaining the Qubit concentration for the library and the mean peak size from Agilent Tape Station profile, the PE Illumina library was loaded onto Illumina for cluster generation and sequencing. Paired-End sequencing allows the template fragments to be sequenced in both the forward and reverse directions on Illumina. The kit reagents were used in binding of samples to complementary adapter oligoes on paired-end flow cell. The adapters were designed to allow selective cleavage of the forward strands after re-synthesis of the reverse strand during sequencing. The copied reverse strand was used to sequence from the opposite end of the fragment.