title: RNA-seq of Sorghum bicolor: Nampungchal seedling leaf
design: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs). mRNA was isolated with the Poly(A) RNA Selection Kit (LEXOGEN), converted to cDNA, indexed, PCR-enriched, and sequenced as paired-end 100 bp reads on an Illumina NovaSeq 6000 platform.