title: 16S rRNA Sequencing of Sphagnum multifibrosumcapitula
design: Sequencing libraries were constructed using 16S rRNA gene amplicons. Total DNA was extracted from frozen samples first. Nested PCR was performed with Q5 High-Fidelity DNA Polymerase (New England Biolabs) to amplify the V6-V9 hypervariable region of 16S rRNA gene (second-round primers: 926F/1392R). PCR products were purified using AXYGEN Gel Extraction Kit, then quantified via Microplate reader FLx800 (BioTek) with Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Final libraries were prepared for paired-end sequencing on Illumina MiSeq platform, using MiSeq Reagent Kit V3 (617 cycles).