title: GSM8717834: 8505c-TRA; Homo sapiens; OTHER
library_construction_protocol: After harvesting the cells from different groups, we extracted the genomic DNA. Library construction for NGS was performed by two-step PCR. The PCR products were purified and then sequenced on a HiSeq 2500 by Azenta Next Generation Sequencing Services. Each library was sequenced at 30–40 million reads to achieve ∼300 × average coverage over the CRISPR library. Library construction for NGS was performed by two-step PCR. The PCR products were purified and then sequenced on a HiSeq 2500 by Azenta Next Generation Sequencing Services. Each library was sequenced at 30–40 million reads to achieve ∼300 × average coverage over the CRISPR library.