title: GSM9381101: Small intestine tissue from mice treated with SM102-LNP (biological replicate 2); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from small intestinal tissue using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. RNA quality and quantity were assessed using  Agilent 2100 Bioanalyzer. mRNA libraries were prepared using the BGI Optimal Dual-Module mRNA Library Preparation Kit. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse transcribed into cDNA. Double-stranded cDNA was end-repaired, A-tailed, adapter-ligated, PCR-amplified, circularized, and converted into DNA nanoballs for sequencing.