title: GSM8941780: WT.All.2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were gently fixed in the strainer and quickly chopped, brains were harvested and dissected immediately on ice and dry ice mixture. Hippocampal dissections were flash frozen in liquid nitrogen and stored at -80C prior to further processing. Paired vascular and non-vascular nuclei preparations were loaded onto a Chromium Next GEM Chip G (10xGenomics, PN-1000120) with libraries prepared using a Chromium Next GEM Single Cell 3' Kit v3.1 following manufacturer recommendations (10xGenomics, PN-1000269).   In total four 5XFAD and four WT mice were profiled, corresponding to 16 total samples (bulk and vascular for each). For each sample we targeted 6000 nuclei on average. Libraries were sequenced on a NovaSeq S4 (Illumina) targeting 60,000 reads per nucleus.