title: WGS of Peromyscus leucopus: ear punch tissue sample
design: DNA was extracted from ear punch tissue samples using Gentra Puregene Tissue Kits. The purity of extracted DNA was assessed by measuring 260/280 absorbance ratios with a NanoDrop Spectrophotometer and sample concentrations were measured using the Qubit dsDNA HS Assay. Prior to library preparation, the presence of high-molecular weight DNA was confirmed using agarose gel electrophoresis. Library preparation and sequencing were conducted by the University of Wisconsin-Madison Biotechnology Center. Sequencing libraries were constructed with the Illumina DNA Library Prep using 100-500 ng of input DNA per sample. Purified and pooled libraries were sequenced on the Illumina NovaSeq6000 platform to generate paired-end 150 bp reads. Each mouse was sequenced across multiple lanes, ranging from 3-10 lanes per individual. Distinct sequencing lanes are denoted in FASTQ filenames by the L00* identifier. A second round of sequencing was conducted for 77 mice using new libraries. Reads generated by this distinct sequencing experiment have FASTQ filenames prefixed with "secondRound".