title: amplicon sequencing of the bacterial V4 region (16S rRNA): low_salinity_root
design: Amplicon sequencing analysis by targeting the V4 region of the of the 16S SSU rRNA gene for prokaryotes (amplified with 515f - 806r primer pair). A two-step amplification protocol was implemented, to construct amplicon libraries. A unique 12-bp index was added to the forward primer to served as a barcode to identify sequencing products in multiplexed libraries. Amplicons were sequenced via MiSeq Illumina v3 2300 bp paired-end reads (Greek Genome Center, Biomedical Research Foundation Academy of Athens (BRFAA))