title: GSM9531710: B16gp_P14. FTY720, lung metastasis, D21; Mus musculus; RNA-Seq
library_construction_protocol: By using fluorescence-activated cell sorting, CD8+ T cells were obtained from the livers or lungs of chronic virus-infected mice and from metastatic lungs/livers of tumor-engrafted mice. Then, the CD8+ T cells were performed with single-cell RNA-seq library construction. For single-cell RNA-seq library construction, the libraries were prepared using Chromium Next GEM Single Cell 3' reagent Kits (10x Genomics) according to the manufacturer's instructions. Briefly, sorted CD8 T cells were suspended in DPBS containing 0.04% BSA and captured in droplets using a Chromium Single Cell Controller instrument (10x Genomics). After reverse transcription and cell barcoding in droplets, cDNA was purified and PCR amplified, followed by fragmentation, end-repair, double-size selection and PCR amplification with sample-indexing primers.