title: GSM9383013: R. parkeri, 0hpi, rep 1, pre-capture; Rickettsia parkeri str. Portsmouth; RNA-Seq
library_construction_protocol: RNA was collected by aspirating cell culture medium and adding 500 µL of Trizol Reagent (Thermo Fisher Scientific 15596026) to each well. Each replicate used material from 1–2 wells. 0 hpi samples were collected by adding purified R. parkeri directly to Trizol Reagent. RNA was extracted using PureLink RNA mini kit (Thermo Fisher Scientific 12183018A) following manufacturer's Trizol extraction protocol. Total RNA from infected host cells was used to create stranded, barcoded cDNA libraries using Twist RNA Library Prep Kit (Twist Biosciences 107061) according to manufacturer's protocol. cDNA libraries from replicates of the same biological condition were pooled together. R. parkeri cDNA was enriched from these pooled libraries using the Twist standard hyb and wash kit v2 (Twist Biosciences 105561), our custom R. parkeri probe panel (Twist Biosciences), and Twist's target enrichment standard hybridization v2 protocol.