title: GSM9166029: PBMCs MPXV infected, 5dpi; Homo sapiens; Orthopoxvirus monkeypox; RNA-Seq
library_construction_protocol: Single-cell RNA-seq libraries were prepared with the 10× Genomics platform using the Chromium Next GEM Single Cell 3' Reagent Kits v.3.1 following the manufacturer's instructions. Single-cell RNA-seq libraries were prepared with the 10× Genomics platform using the Chromium Next GEM Single Cell 3' Reagent Kits v.3.1 following the manufacturer's instructions. Quality control of the libraries was performed with the KAPA Library Quantification Kit and Agilent TapeStation. Libraries were sequenced on a HiSeq4000 using the following sequencing mode: read 1: 28 bp, read 2: 91–100 bp, Index i7: 8 bp. The libraries were sequenced to reach ∼20,000 reads per cell.