title: GSM9162751: t_3h_FPL, rep 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using the PurelinkTM RNA Mini Kit (Thermo, Cat no. 12183025) according to the manufacturer's instructions. Library preparation was conducted according to the Illumina Stranded mRNA Prep reference guide (Illumina, Cat no. 1000000124518 v02). PCR cycles were adjusted based on RNA input (12 and 11 cycles for 3h and 24h samples, respectively). A pool of 24 libraries with paired-end and 50 bp-long reads was sequenced on the NovaSeq system (Illumina). The average number of reads per sample was approximately 25 million. There are 6 replicates for each sample group.