title: epidural tissues
design: Single-cell suspensions (1105 cells/ml) with PBS (HyClone) were loaded into microfluidic devices using the Singleron Matrix Single Cell Processing System (Singleron). Subsequently, the scRNA-seq libraries were constructed according to the protocol of the GEXSCOPE Single Cell RNA Library Kits (Singleron ) 66. Individual libraries were diluted to 4 nM and pooled for sequencing. At last, pools were sequenced on Illumina novaseq6000 with 150 bp paired end reads.