title: GSM9162699: fetal brain, trisomy 21, Library B12D1; Homo sapiens; RNA-Seq
library_construction_protocol: Fresh-frozen fetal tissue from surgical terminations of pregancy from UK hospitals was collected by the "Human Developmental Biology Resource" (HDBR, https://www.hdbr.org/). Total RNA was extracted from ~2-10 mg of fetal human brain tissue using the RNeasy Plus Micro kit (Qiagen) according to manufacturer's instructions. RNA-seq was then performed by the NIHR Imperial BRC Genomics Facility using the NEBNext rRNA Depletion kit v2 (Human/Mouse/Rat) and NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina. Libraries were sequenced in PE75 mode.