title: human blood
design: Whole-exome sequencing of human blood DNA was performed to identify germline/somatic variants associated with Isolated frontosphenoidal craniosynostosis. Genomic DNA was extracted from one peripheral blood sample. Exonic regions were enriched using IDT xGen Exome Research Panel v1.0 and libraries were prepared following the manufacturers protocol. Paired-end 150 bp sequencing was carried out on an Illumina HiSeq 4000.