title: GSM9207337: CnR_HMGN1_rep1; Homo sapiens; OTHER
library_construction_protocol: Cut and Run samples for HMGN1-Flag. Cardiomyocytes differentiated from human induced pluripotent stem cells (hiPSCs) were collected at day 20 for CUT&RUN analysis. Cells were dissociated using 0.25% Trypsin-EDTA (1X) (Gibco, 25200-072) and neutralized with 1% FBS in PBS. For each condition, 150,000 cells per sample were processed according to the manufacturer's instructions using the CUTANA™ CUT&RUN Kit (Epicypher, 14-1048). Cells were first washed and bound to activated Concanavalin A (ConA)–coated magnetic beads in the Bead Activation Buffer. Nuclei were permeabilized with Digitonin-containing buffer, and all buffers were supplemented with cOmplete™ Mini, EDTA-free Protease Inhibitor Cocktail (Millipore Sigma, 11836170001). CUTANA CUT&RUN Epicypher