title: GSM9380847: Het 1 5 dpf; Danio rerio; RNA-Seq
library_construction_protocol: RNA was extracted from composite samples of 4-6 whole zebrafish using RNAzol reagent Performed by NovoGene Corporation as follows: "Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. Then the second strand cDNA was synthesized using dUTP, instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification."