title: RNA seq of Pomacea canaliculata: Haemocytes
design: Full-length cDNA libraries were prepared from 200 ng of total RNA using the Oxford Nanopore cDNA-PCR Sequencing Kit (SQK-PCS111). Following reverse transcription and strand-switching, transcripts were amplified via 14 PCR cycles to select full-length sequences. Purified cDNA (AMPure XP beads) was quantified via Qubit and checked with Agilent TapeStation D5000. 25 fmol of the final library were loaded onto a FLO-MIN106 flow cell and sequenced on a MinION Mk1C for 64 hours.