title: GSM9137205: Primary keratinocytes (strain Km), 12h of culture, S1 substrate, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the RNeasy mini kit (Qiagen). Each experiment was performed with three technical replicates per condition. Technical replicates were pooled together. The quality of the RNA was checked on a Qubit 2.0 Fluorometer (Thermo Fisher).Samples submitted for sequencing had an RNA integrity number of 9.9-10.0 Messenger RNA (mRNA) selection, library preparation and sequencing were performed by GENEWIZ Inc., as described (Cujba et al., 2022) standardized Illumina protocols.