title: Floral composition of Vespula vulgaris nests through chloroplast metabarcoding
design: Amplicon sequencing of the P6 loop of trnL (UAA) intron was conducted on an Illumina HiSeq Platform (Illumina, San Diego, USA) by Azenta Life Sciences (Suzhou, China). Amplicons cover a short fragment (30-143 bp) of this intron. Automated cluster generation and 250 paired-end sequencing with dual reads were carried out following the manufacturers instructions. Double-end sequencing was then performed on both positive and negative reads, with the initial read joining. Sequences containing ambiguous bases (sequence of N) were filtered out, retaining only sequences > 50 base pairs.