title: 16s rRNA seq of Nilaparvata lugens: F0 control R2
design: DNA was extracted from whole insect bodies using the Qiagen DNeasy Blood & Tissue Kit. The V3V4 region of the 16S rRNA gene (~459 bp) was PCR-amplified using region-specific primers with Illumina overhang adapters. Amplicons were purified using Ampure XP beads and indexed with Nextera XT Index Kit. Libraries were checked using Agilent TapeStation 4150, quantified via Qubit HS and qPCR, and sequenced using the Illumina MiSeq PE300 platform.