title: VisiumHD spatial transcriptomics of untreated ASS-ILD patient
design: The Visium HD Spatial Gene Expression platform (Visium HD, 10X Genomics), capable of achieving single-cell scale spatial resolution, was utilized in this study. In accordance with the Visium HD FFPE Tissue Preparation Handbook (CG000684, 10X Genomics), tissue sections of 10 m were prepared from FFPE blocks, ensuring high RNA quality (DV200  30%). After H&E staining, probes were hybridized, ligated, eluted, and subsequently used for library construction based on the Visium HD Spatial Gene Expression Reagent Kits User Guide (CG000685). The resulting libraries were sequenced on the Illumina NovaSeq X platform using a paired-end approach.