title: GSM9566557: empty vector biol rep1; Listeria seeligeri; RNA-Seq
library_construction_protocol: Cultures were pelleted via centrifugation and pellets were resuspended in 100 μL of 1X PBS with 2 mg/mL lysozyme and incubated for 5 min in a 37°C water bath. After incubation, 10 μL of 10% sarkosyl was added to each sample and resuspended before adding 300μL TRI reagent. Samples were vortexed and 400μL of 100% ethanol was added. Each sample solution was then purified using the Zymo Direct-zol RNA Miniprep Kit to the manufacturer's instructions. RNA-extraction samples were treated with DNAse and column purified using the Zymo RNA Clean & Concentrator kit per the manufacturer's instructions. Performed by Azenta (Strand-specific RNA-seq) including rRNA depletion