title: GSM8859660: SPL9OE_Cu_excess_Rep2; Oryza sativa Indica Group; ChIP-Seq
library_construction_protocol: Sheared chromatin from 21 day old seedlings of 3XFlag:SPL9OE (T2 generation) were grown in appropriate copper concentrations and immunoprecipitated with Anti-FLAG M2 Magnetic Beads-M8823. Washes, elution, de-crosslinking and DNA.  purification was done as described. The purified DNA was taken for library preparation. . The ChIP library was constructed using NEBNext® Ultra™ II DNA Library Prep with Sample Purification Beads (Catalog no-E7103L) as per manufacturer's instructions. The adapter sequence used AGATCGGAAGAGCACACGTCTGAACTCCAGTCA and AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT