title: Treated1
design: Leaf tissues of biostimulant treated (through seed priming) and control plants were harvested from 90 days old plants (72 hours after 250mM salt treatment) and stored in RNA stabilizing solution (SRL BioLit 71969) following manufacturers protocol until the RNA extraction process was carried out. Extraction was carried out by Trizol method. Extracted RNA quantity is checked on Qubit flurometer (Thermofisher #Q33238) using RNA HS assay kit (Thermofisher #Q32851) following manufacturers protocol. To measure the purity of the extraction we also measured the concentration of the samples on Nanodrop 1000. Finally, to obtain the RIN values of the samples, Tapestation 4150 using HS RNA screen tape was utilized. The library preparation was carried out using SMART-Seq Library Prep Kit (R40047). Final libraries were quantified using Qubit 4.0 fluorometer (Thermofisher #Q33238) using DNA HS assay kit (Thermofisher #Q32851) following manufacturers protocol. To identify the insert size of the library, it was queried on Tapestation 4150 (Agilent) utilizing highly sensitive D1000 screentapes (Agilent # 5067- 5582) following manufacturers protocol.