title: RNA-seq of Embryonic Chicken Forelimb Bud (HH25)
design: After limb dissection in PBS, left and right side limbs were lysed with Trizol, and RNA was extracted using 5PRIME Phase Lock Gel Heavy tubes (Quantabio) followed by RNeasy micro (Qiagen) cleanup. All total RNA was diluted to 2 ng/uL and 50 uL were provided to the Harvard Bauer Core for Kappa Hyperprep RNA-seq Library synthesis with poly-A selection (Roche).  Insert sizes of 200-300 were requested and 13 cycles of PCR utilized.  All libraries were sequenced on a Nextseq High 150 (Illumina) as paired-end 37bp reads.