title: GSM9055877: mESCs, IP; Mus musculus; RIP-Seq
library_construction_protocol: Cells were harvested and cross-linked with 1% formaldehyde at room temperature for 10 minutes. The reaction was quenched by adding 1ml of 1.25 M glycine and incubated at room temperature for 5 min, and then resuspended with RIP lysis buffer (50 mM HEPES (pH 7.5), 0.4 M NaCl, 1 mM EDTA, 1 mM DTT, 0.5% TritonX-100,10% Glycerol, 0.1 M PMSF, 1.52 mg/ml protease inhibitor cocktail, 40 U/μl RNase inhibitor). Anti-FLAG M2 magnetic beads (Sigma-Aldrich, M8823 were used for immunoprecipitation. Library construction and sequencing were conducted by Novogene in Tianjin, China.