title: Expression of the the sepiapterin reductase (SPR) gene based on RNA-seq data from throat skin collected from outside of the main color patch for a Uta stansburiana: Orange throat-color morph
design: The focal tissue was throat skin just outside of the main colored region, which was typically white in color, but which sometimes had a very faint amount of the color found in the main color patch. RNA was extracted using a New England Biolabs Monarch Total RNA Miniprep kit.  Libraries were prepared with a KAPA (Roche) stranded mRNA-Seq Kit with unique dual indexes.   The sequence data was cleaned with HTStream and mapped with STAR to the Uta stansburiana orange-morph genome.  The bam file is a region from positions 1020000-1040000 on the contig ptg000029l, which encompasses the SPR gene.  The SPR gene, including 5prime and 3prime untranslated regions is between positions 1025607-1031171.  The positions of the coding sequence of SPR are between positions 1030545-1030902, 1027502-1027792, and 1026365-1026555.