title: GSM9380483: bulk, baseline, E9, RNA; Homo sapiens; RNA-Seq
library_construction_protocol: for bulk RNA-Seq, total RNA was extracted using miRNeasy Mini Kite and homogenized in Qiazol using stainless steel beads. Chloroform was then used for phase separation. RNA containing an aqueous layer was further purified using the RNeasy MinElute spin column. RNA was finally eluted in RNase-free water. for sn-multi-omics, nuclei were isolated using the Chromium Nuclei Isolation Kit. Nuclei were directly processed for droplet capture for single cell multiome ATAC + gene expression using a chromium controller. For bulk RNA-seq, librairies were prepared using Ribo-Zero gold sample preparation kit. Library concentrations were measured using KAPA Illumina Library Quantification Kit. Pooled libraries were sequenced on Illumina NovaSeq6000 sequencer with 150-bp Paired-End chemistry. For sn-multi-omics, Chromium Next GEM Single Cell Multiome ATAC + Gene v1 chemistry was used to create single nuclei ATAC and RNA libraries from the same cell. Libraries were sequenced on an Illumina Novaseq 6000 with a 150 bp paired end read setup.