title: Whole genome illumina sequencing of Melinaea marseus adult thorax tissue
design: DNA extractions were carried out using the Qiagen DNeasy Blood and Tissue Kit, Qiagen MagAttract High Molecular Weight kit, QiaAmp DNA mini kit, or a PureLink digestion and lysis step followed by a magnetic bead DNA extraction52. DNA concentration was quantified using Qubit Fluorometer (Invitrogen) and purity assessed using NanoDrop (Thermo Fisher Scientific). For each sample, 350 bp fragment libraries were made using NEBNext Ultra II FS Kit, or using TN5-transposase-mediated tagmentation (adapted from53), or following the manufacturers guidelines with the Illumina DNA PCR-free library prep kit and sequenced (150 bp paired-end) on Illumina NovaSeq 6000 or NovaSeq X machines.