title: 16S rRNA of mus musculus: weaned male feces
design: This study aimed to investigate the impact of early-life overfeeding (ELOF) on the gut microbiota and its role in metabolic imprinting. We performed PacBio long-read, full-length 16S rRNA gene sequencing on fecal samples collected from a mouse model of ELOF and their normal-diet controls. The goal was to achieve species-level resolution of the microbial community structure to identify specific taxa, such as Lactobacillus johnsonii, that are associated with ELOF-induced metabolic disorders.