title: GSM9206987: CUTRUN_H3K4me3_ETV3WT_01; Mus musculus; OTHER
library_construction_protocol: CUT&RUN was performed on ~2.5x105 cells using the CUT&RUN Assay Kit (Cell Signaling Technology) according to the manufacturer protocol. Briefly, cells were washed and immobilized with Concanavalin A beads. 2 µL of H3K4me3 (C42D8) Rabbit mAb was added to each sample and incubated overnight. The tubes were washed, pAG-MNase enzyme was added, and activated with calcium chloride. DNA was purified using DNA Purification Buffers and Spin Columns (Cell Signaling Technology). Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) according to the manufacturer protocol.