title: GSM8717761: UM-UC-3 cells, shCtrl, Xenograft, 1-5; Homo sapiens; Mus; RNA-Seq
library_construction_protocol: Ctrl and CDH11 KD cell lines were precultured in 12-well plate (2 × 105 cells/well) for 24 hours. Total RNA samples were extracted using RNeasy spin column kits and DNase I. Ctrl and CDH11 KD cell lines were suspended in Matrigel solution (Corning; 1 × 107 cells/ml). The cell suspension (100 μl) was inoculated subcutaneously into 5-week-old nude mice. Tumor tissues were sampled at 4 weeks after transplantation. Tissue pieces sampled from the Xenograft model were lysed using ISOGEN. Total RNA was extracted using RNeasy spin column kits and DNase I. RNA integrity number was verified using an Agilent 2100 Bioanalyzer.RNA-seq was performed on an Illumina NextSeq 500 with a read configuration of 75 bp for single reads; 3 million reads were generated per sample. In xenograft model, RNA-seq was performed on the NovaSeq6000, with a read configuration of 75 bp for single reads; 3 million reads were generated per sample.