title: Tridacna crocea egg
library_construction_protocol: Total RNA was extracted using a POLYTRON Homogenizer (Kinematica)  in combination with the Direct-zol RNA Miniprep Kit (Zymo Research). Intact poly(A)+ RNA was isolated with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA-seq library was then prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs) (New England Biolabs).