title: GSM9306954: M. tuberculosis H37Rv wild-type untreated biological replicate 1; Mycobacterium tuberculosis H37Rv; RNA-Seq
library_construction_protocol: Bacterial pellets (15 mL culture) were resuspended in TRIzol with 0.1 mm zirconia beads and lysed using a Precellys Evolution homogenizer (3 cycles, 30 seconds at 7,400 rpm). RNA was extracted with chloroform, precipitated with ethanol, and purified on RNeasy columns (Qiagen) with on-column DNase I digestion. RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit with polyA selection following manufacturer's protocols. Libraries were sequenced on Illumina NovaSeq X Plus platform (2×150 bp paired-end reads).