title: GSM9531569: Vehicle blastocysts, control, rep 1; Bos taurus; OTHER
library_construction_protocol: Total DNA (nuclear and mitochondrial) was extracted from all samples using the Quick DNA Microprep kit (Zymo Research) after a proteinase K digestion (50mg/mL, 50oC, 20min). Cytosines present in total DNA samples were enzymatically converted prior to library preparation using the NEBNext® Enzymatic Methyl-seq Kit (New England Biolabs). The protocol followed the manufacturer's instructions, including the addition of positive (puc19) and negative (lambda DNA) methylation controls to allow the calculation of conversion efficiency. The quality and quantity of each library was verified with Agilent TapeStation. Sequencing was performed in one lane of NovaSeq 6000 PE 150 bp (Illumina).