title: GSM9380033: HeLa, RO3306, actD, 1 h, bio rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed in PBS and resuspended in TRIzol Reagent. All RNA isolations were performed with Phasemaker™ tubes according to the manufacturer's protocol (Invitrogen, A33248). Total RNA was resuspended in water with 4 pg spike-in RNA (equal mixture of in vitro transcribed Fluc and Nluc) per 2 ug total RNA for experiments where spike-in RNA was used for normalization. For each sample, 500 ng total RNA was used for library prep. For NOVASEQSP sequencing runs, libraries were prepared using the KAPAHyperRiboErase kit according to manufacturer's instructions. For AVITI sequencing runs, libraries were prepared using the Watchmaker QiaSeq FastSelect Ribodepletion kit according to manufacturer's instructions.