title: Mystus gulio genome, HiFi reads, Pacbio Sequel II, Library 1
design: High molecular weight genomic DNA was isolated from muscle tissue of a single female fish using QIAGEN Genomic-tip 100/G Midi kit (Qiagen, Hilden, Germany). DNA shearing was performed on Megaruptor 3 system (Diagenode, Belgium). Sequencing library was constructed using SMRTbell Express template Preparation Kit 3.0 (Pacific Biosciences, California, USA). The libraries were size selected using AMPure PB beads (Pacific Biosciences, California, USA). The library was loaded onto 8M SMRT cell and sequenced on PacBio Sequel II system in CCS/HiFi mode.