title: GSM9210278: HepG2 CS RNAi; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was harvested using Trizol reagent. 1 μg total RNA was used for library preparation. Poly(A) mRNA was isolated using oligo(dT) beads.  mRNA was fragmented with divalent cations and high temperature. Random Primers were used to prime cDNA synthesis, and first strand cDNA and the second-strand cDNA synthesized. Double-stranded cDNA was treated to repair both ends and add a dA-tail, followed by a T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was then performed using DNA Clean Beads. .Each sample was amplified by PCR using adapter sequence primers at both ends.  Libraries were multiplexed and loaded on an Illumina HiSeq/ Illumina Novaseq/ MGI2000 instrument for sequencing using a 2x150 paired-end (PE) configuration according to manufacturer's instructions