title: WGS of Saccharomyces cerevisiae CRI-SPA-Map isolates
design: Genomic DNA was isolated in six 96-well plates after zymolyase treatment using a Quick DNA 96 Plus Kit from Omega. The DNA was tagmented (Illumina, Nextera kit) and then PCR amplified using unique Illumina i5 and i7 index primer pairs. Samples from each 96-well plate were pooled and size fractionated by gel-electrophoresis to obtain fragments in the 450550 bp size range. The 6 size-fractionated pools where combined in equal concentration and sequencing of 150-bp paired-end reads was performed on an Illumina NovaSeq 6000.