title: 50:50 mixture of U937 and K562 cells treated with 0.5μg/ml of LPS, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags
design: 50:50 mixture of U937 and K562 cells treated with 0.5μg/ml of LPS, profiled with ChronoSeq for 12 samples barcoded with unique Time-Tags
library_construction_protocol: Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html Cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C. https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html 0.5ug/ml LPS manually added to Cell Sample Reservoir 5 minutes after first sample/time-tag Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together, then split into four equal parts for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html