title: GSM8718547: HME_MSL1_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using a RNeasy Plus Micro Kit (Qiagen, 74034) For total experiment, ribosomal RNA removal was performed with FastSelect -rRNA HMR Kit (Qiagen, 334386) and libraries were prepared using NEBNext UltraII Directional RNA Library Prep Kit (NEB, E7760). For spike-in experiment, RNA concentration was checked with the nanodrop. 1ug of RNA was taken from the lowest concentrated sample and same volume was taken for all the other samples. 2 μL (1:100) ERCC Spike-in (Thermo Fisher Scientific, 4456740) was added to each sample. Libraries were prepared using NEBNext UltraII Directional RNA Library Prep Kit (NEB, E7760) with polyA enrichment.