title: GSM8720261: MCF7 Everolimus-Resistant, DMSO, Bio rep 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were collected for bulk RNA-seq  after 12 hours of treatment. Cells were pooled, washed with PBS, and pellets were frozen at -80°C for subsequent RNA isolation. RNA was isolated using the Qiagen RNeasy® Plus Mini Kit (Cat. No: 74136) following the manufacturer's protocol. Library preparation was performed on the samples using the Illumina TruSeq Stranded mRNA Kit following the manufacturer's protocol.