title: GSM9381610: Huh7 cells, expressing wild-type SIRT6 - rep1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. Indexed RNA-seq libraries were prepared from 250 ng of polyA-selected mRNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs).