title: GSM9162198: scSLAM-seq of adult zebrafish hearts, injured, 6hpci replicate 2; Danio rerio; RNA-Seq
library_construction_protocol: Zebrafish hearts were dissociated in HBSS supplemented with Pluronic-F68 and liberase (Roche). The cell suspension was then fixed in methanol and thiol modification performed by incubation in iodoacetamide overnight. A detailed protocol can be found in the source publication. Libraries were constructed following manufacturers instructions of the 10X Genomics 3' GEX NextGEM v3.1 protocol.