title: H3K27Ac C&R in LNCaP etoh treated replicate 1
design: Library selection was performed using CUT&RUN approach. CUT&RUN experiments were performed as instructed by the manufacturer (Cut&Run Assay Kit, Cell Signaling Technology, USA), using 100,000 cells per condition, in experimental duplicates. The antibodies used and their concentrations are described in the Supplementary Methods. As recommended in the kit, purified spike-in DNA from Saccharomyces cerevisiae was added to the samples, and DNA input samples were generated through sonication. Samples were purified with the kit DNA Purification buffers and spin columns (ChIP, Cut&Run) (Cat. #14209, Cell Signaling Technology). Sequencing libraries were prepared with the kits SimpleChIP ChIP-seq Multiplex Oligos for Illumina (Cell Signaling Technology) and DNA Library Prep Kit for Illumina (Cell Signaling Technology #56795). Sequencing was performed in a DNBSEQ platform (BGI Genomics, China), with 50bp pair-ended sequencing for CUT&RUN samples and 100bp pair-ended sequencing for input samples.