title: GSM9258448: Human T cells_TP53_WT5_rep12; Homo sapiens; RNA-Seq
library_construction_protocol: RNA isolation and library preparation were performed according to the Prime-seq protocol. RNA was isolated from cell lysates and cDNA synthesized using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific, EP0753) with barcoded oligo-dT primers (E3V7NEXT) and a custom template-switching oligo. Libaries were prepared from 30 ng of pooled cDNA. Libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, E7805S) using a prime-seq specific custom ligation-adapter and dual-indexing with TruSeq i5 and Nextera i7 primers via PCR.