title: 16S rRNA sequencing of human fecal microbiota
design: Total DNA was isolated from the individual human donors, the pooled inoculum, and the 48 h in vitro fermentation samples using the FastDNA SPIN Kit for Soil (MP Biomedicals, Solon, OH), following the manufacturers protocol. The extracted DNA was then used for high throughput 16S rRNA gene sequencing on an Illumina MiSeq platform, performed by RTL Genomics (Lubbock, TX)