title: GSM9678628: Mouse 7, Tumor 2, high dose DAC; Mus musculus; OTHER
library_construction_protocol: Total RNA was purified from whole tumors using TriReagent and EconoSpin Columns. Tumors were homogenized by adding 2.8 mm Zirconium beads to the tissue, and homogenization was carried out using a Precellus Homogenizer run at 3 x 15 seconds at 6500 rpm. TCRa and TCRb receptor libraries covering the complemen-tarity determining region 3 (CDR3) were prepared using SMART-Seq Mouse TCR (with UMIs) (Takara, 634815) and SMARTer RNA Unique Dual Index Kit (Takara, 634451) following manufacturer's instructions.