title: GSM9163365: RNA-iMGT-Myh6-eGFP-MEFs, siControl, D3, BiolRep3; Mus musculus; RNA-Seq
library_construction_protocol: On day 0 post-siRNA knockdown, day 1 post-MGT overexpression, and day 3 post-MGT overexpression, RNA was extracted from the cells using the Zymo Research Quick-RNA MircoPrep Kit (#R1051) following the manufacturer's recommendations.Cells were pooled from 40 wells per sample for each biological replicate. Library preparation was performed by the La Jolla Institute for Immunology Next Gen Sequencing core using NEB poly-A kit mRNA-seq Library Prep following the manufacturer's recommendations.