title: RNA-Seq of Coscinodiscus radiatus
design: C. radiatus cells were collected during light cycle, filtered through 40 m and 10 m membranes, and treated with TRIzol reagent for RNA extraction. After incubation and centrifugation, diatom frustules were pelleted. RNA was extracted using the Direct-zol RNA Miniprep kit and assessed for concentration and quality with Bioanalyzer and Qubit assays.