title: Streptomyces isolate 4 16S sequencing
design: Genomic DNA was extracted from pure cultures of *Streptomyces* isolates grown on nutrient agar using a bacterial genomic DNA extraction protocol. DNA quality and concentration were assessed using spectrophotometry and agarose gel electrophoresis. Sequencing libraries were prepared using an Illumina-compatible library preparation kit following the manufacturers instructions. Briefly, genomic DNA was fragmented, end-repaired, A-tailed, and ligated with sequencing adapters containing unique indices. The libraries were purified and size-selected to obtain an average insert size of approximately 350 bp. Library quality and concentration were verified prior to sequencing. Paired-end sequencing was performed on an Illumina platform to generate high-quality reads for whole-genome analysis of the *Streptomyces* isolates.