title: RNA-Seq of cerebral: 20-70d
design: Library enrichment: PCR amplification for 15 cycles; Target band recovery using 2% agarose gel electrophoresis; Quantification with TBS380 (Picogreen) and mixing based on data ratio for sequencing; Bridge PCR amplification on cBot to generate clusters; Sequencing on Illumina platform (PE library, read length 2150 bp).