title: GSM9161372: HCT116-3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Total RNA Extractor（Trizol）kit (B511311, Sangon, China) according to the manufacturer's protocol, and treated with RNase-free DNase I to remove genomic DNA contamination. RNA integrity was evaluated with a 1.0% agarose gel. Thereafter, the quality and quantity of RNA were assessed using a NanoPhotometer ® spectrophotometer (IMPLEN, CA, USA) and a Qubit® 2.0 Flurometer (Invitrogen). The high quality RNA samples were subsequently submitted to the Sangon Biotech (Shanghai) Co., Ltd. for library preparation and sequencing. Sequencing libraries were generated using VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® following manufacturer's recommendations and index codes were added to attribute sequences to each sample