title: GSM8546956: HCT116 DP1 CRISPR clone 1, biological replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using TRIzol, as per the manufacturer's instructions RNA from MCF7 cells was subsequently enriched using the NEBNext Poly(A) mRNA magnetic isolation module as per the manufacturer's instructions. cDNA libraries were made using the NEBNext ultra directional RNA library prep kit for Illumina, as per the manufacturer's instructions. RNA from HCT116 cells was used to prepare libraries by BGI Genomics.  Briefly, mRNAs were isolated using oligo(dT)-based mRNA enrichment.  mRNA was fragmented and reverse transcribed with random primer, prior to second strand synthesis with dUTP, end repair and 3' A-tailing. This was followed by adaptor ligation and digestion of U-labelled second strand template with UDG, prior to PCR amplification. Library quality control was performed before circularisation and amplification to make DNA nanoballs.