title: 18srRNA-seq of Gull
design: Total DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, Carlsbad, CA, USA), following the manufacturers instructions. The 18S rDNA V9 region was amplified by polymerase chain reaction (PCR) using the primers 1391f (5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGGTACACACCGCCCGTC-3) and EukBr (5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGTGATCCTTCTGCAGGTT CACCTAC-3)