title: Transcriptomic analysis of nuclear receptor(LXR, FXR) modulation, mTOR inhibition, and nutrient stress on human HepG2 cells
design: Transcriptomic analysis of nuclear receptor(LXR, FXR) modulation, mTOR inhibition, and nutrient stress on human HepG2 cells
library_construction_protocol: HepG2 cell line was ordered from ATCC (HB-8065) and cultured. Cells were harvested directly from cell culture and no dissection was perormed. HepG2 cells were cultivated in DMEM (Gibco, 41965) containing 10% FBS (Gibco, 10500064) and 1% penicillin/streptomycin (Gibco, 15140122) Cells were treated with treated with GW3965 (5µM), GW4064 (5 µM) alone or combined with mTORC1 inhibitor Torin 1 (TOI), or combined with glucose/glutamine starved media (GLUST). DMSO with final concentration of 0.1% (v/v) was used as control treatment.  RNA was extracted using Trizol reagent (Invitrogen, 15596026) according to the manufacturer's instructions. QuantSeq 3' mRNA library preparation kit (Lexogen) according to manufacturer's instructions.  