title: L. serriola US96UC23 ONT reads
design: Lactuca serriola (US96UC23) seedlings were grown in Magenta boxes under sterile conditions in the dark. Seven-day-old etiolated seedlings were harvested and flash frozen in Liquid N2. Etiolated tissue was used for DNA extraction using a CTAB DNA extraction/Sorbitol cleanup protocol. ONT libraries were prepared with the LSK109 kit. ONT sequencing was performed using PromethION and base called with Guppy(v5_0_16_sup)