title: Long-read sequencing of Antarctic soils
design: For the long-read data, genomic DNA was submitted to KeyGene (Wageningen, the Netherlands) for Oxford Nanopore sequencing. Quality control included DNA quantification and size assessment using the Femto Pulse system. Purified DNA was used for library preparation with the SQK-LSK110 kit and sequenced on a FLO-PRO002 (R9.4.1) flow cell using the PromethION P24 platform over 72 hours.