title: Transcriptome Sequencing of Human Sample Pt_165_baseline
design: Transcriptome sequencing of 20 human individuals from diverse ethnic backgrounds to identify rare genetic variants associated with atezolizumab + bevacizumab treatment-refractory metastatic HPV+ anal cancer, using Nimblegen_RNA_SeqCap_V3 library preparation kit and  Illumina NovaSeq 6000 platform and STAR for read alignment to the GRCh37 reference genome.