title: GSM9161329: Bone Marrow Gene Expression library; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow was harvested from femurs and tibias, and LSK cells were isolated using fluorescence-activated cell sorting (FACS) based on the Lin⁻Sca-1⁺c-Kit⁺ phenotype. After sorting, the LSK cells are incubated with Hash-Tagging Oligonucleotides (HTOs). These HTOs contain a cell-specific barcode, allowing for multiplexing of samples. The cells are then pooled together.The sorted cells were then sequenced on an Illumina NovaSeq 6000 platform.