title: RNA-seq of human breast cancer cell line MDA-MB-231 treated with T025 against untreated controls
design: RNA-seq of human breast cancer cell line MDA-MB-231 treated with T025 against untreated controls
library_construction_protocol: A total of 30 million MDA-MB-231 cells were seeded in a 6-well plate. The following day, cells were treated with 1 ÂµM T-025 or 0.1% DMSO for 24 hours. Total RNA was isolated using the RNeasy plus mini kit (Qiagen). Samples from 3 biological replicates were used. RNA quality and quantity were measured on an Agilent-4200 Bioanalyzer. Stranded PolyA-selected libraries were prepared using the DNBseq platform.