title: Human lung cancer organoid mRNA seq: osimertinib-resistant organoids
design: Total RNA was processed at Xiamen HaploX Co., Ltd. and subsequently sequenced on an Illumina HiSeq X Ten sequencer. RNA reads were mapped, and reads per kilobase of transcript, per million mapped reads were calculated. Differentially expressed genes (DEGs) were defined using the edgeR package with false discovery rate-corrected P  0.05 and fold change  2. The clusterProfiler R package was used to conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of DEGs.