title: Whole genome sequncing of Erysipelotrichaceae bacterium isolated from a human-derived FMT product
design: Bacteria from FMT product isolated on YCFA agar. Resuspended in 300ml sterile PBS and placed in a PowerbeadPro tube (QIAGEN). Lysed by bead beating (Precellys 24 5000 2 x 20 seconds). DNA was purified from 50ml of lysed solution using 30ml of Illumina Purification beads (20060059) and eluted in 30ml of Nuclease Free Water. Illumina libraries were prepared using Illumina DNA Prep (20060059) at quarter reaction volumes and sequenced on a NextSeq2000 P4 XLEAP-SBS Reagent Kit (300 Cycles) (20100992)