title: RNAseq of Wolffia brasiliensis clone 7522
design: Approximately 100 mg of plant material from W. brasiliensis was flash-frozen and ground to a fine powder using 1mm glass beads (Carl Roth, #A554.1) on a SilamatS6 (Ivoclar Vivadent). RNA was then extracted with the Rneasy Plant Mini Kit (Qiagen#74904) was used following manufacturers instructions. Illumina short read libraries were prepared using the NEBNext. Ultra II RNA Library Prep Kit for Illumina following manufacturers instructions