title: TauP301L transgenic
design: The Illumina NovaSeq PE150 sequencing strategy was utilised to generate one lane of sequencing data, yielding approximately 120 gigabytes of raw data. The raw image files from high-throughput sequencing were processed using CASAVA for base calling, resulting in sequenced reads stored in FASTQ format. FASTQ is a prevalent text format for the storage of biological sequences alongside their corresponding quality scores. In data generated by the Illumina NovaSeq PE150 platform, both paired-end reads are 150 base pairs (bp) in length. For 10X Genomics libraries, the first 26 base pairs of read 1 contain cell barcode and UMI (unique molecular identifier) information, which is crucial for distinguishing the origin of read 2. It is considered that the bases following the initial 26 base pairs in read 1 are non-informative. In read 311 bp are regarded as informative data.