title: GSM8655319: patient-derived organoid , PDO_siCTRL_rep2; Homo sapiens; RNA-Seq
library_name: GSM8655319
library_construction_protocol: RNA-seq (n=2 for siCTRL, n=2 for siEZH2, n=2 for siADAR1 and n=2 for siADAR2) was isolated from patient-derived organoid were used to detect RNA editing. To analyze the impact of EZH2 inhibitor on RNA editing, DMSO, EPZ6438 and GSK126 treated C4-2 cells were used to do high depth RNA-seq. eCLIP-seq of igG were profomed to use as additional control for RNA binding target in C4-2. The RNA was isolated using RNeasy Plus Mini Kit (Qiagen) and subjected to admerahealth for library preparation and sequencing using the Novaseq platform. RNA samples were harvested 72 hours post-second knockdown. RNA was isolated using the Qiagen miRNA isolation kit, and knockdown was confirmed by RT-qPCR before sending samples for sequencing. For high-throughput sequencing of RNA-seq and IgG RNA samples were shipped to Admera for library preparation and sequencing using the Novaseq platform.