title: GSM8548422: Treg, VDJ-seq, mRNA-derived cDNA; Mus musculus; OTHER
library_name: GSM8548422
library_construction_protocol: Cells were sorted into 0.04% BSA/PBS, pelleted, and then resuspended at 1000-1500 cells/ul Library was prepared using Chromium Single Cell 5ʹ v3 protocol with V(D)J enrichment for immune profiling (10x Genomics). For T-cell receptor (TCR) library construction, TCR transcripts were enriched from amplified cDNA by PCR. Subsequently, enriched PCR product was fragmented and end-repaired, size-selected, PCR-amplified with sample-indexing primers, and size-selected. For antibody-derived tagged library, we followed the protocol provided by BioLegend (HTO, TotalSeq-A)