title: GSM8792655: SMG1i, Iso-seq, unlabeled; Homo sapiens; OTHER
library_name: GSM8792655
library_construction_protocol: Total RNA was extracted using Trizol Reagent and TimeLapse chemistry was performed as described (Schofield et al., 2018). TL-seq: rRNA depletion was performed by oligo hybridization and digestion with thermostable RNase H (Baldwin et al, 2021). 100 ng of ribodepleted RNA was used as input for the NEBNext Ultra II Directional Library Prep Kit. Iso-seq: 2 µg total RNA was used as input for the Clontech SMARTer PCR cDNA Synthesis kit, followed by PCR amplification. SMRTbell libraries were generated using the SMRTbell Template Prep Kit.