title: GSM8861792: M17_2D_Dy5_2; Mus musculus; RNA-Seq
library_name: GSM8861792
library_construction_protocol: All samples were collected and total RNA of each sample was isolated using the GeneJET RNA Isolation Kit (Fisher Scientific). The samples underwent library preparation and bulk RNA sequencing by Novogene Corporation. CASAVA base recognition (Base Calling) was used to convert original image data files from Illumina sequencing platform to sequenced reads in FASTQ format. A series filtration criteria was applied based on uncertain nucleotides constituting more than 10% of either read (n>10%) and with low-quality nucleotides (base quality less than 5) constituting more than 50 per cent of the read. Next, alignment of raw reads was performed to reference genome using HISAT2. To estimate gene expression levels, the fragments per kilobase of transcript sequence per million base pairs sequenced (FPKM) was quantified for each aligned read. This accounted for both sequencing depth and gene length on counting of fragments.