title: GSM8895451: RM1 macrophages; Mus musculus; RNA-Seq
library_name: GSM8895451
library_construction_protocol: Total RNA was extracted from polarized and irradiated macrophages using WelPrep™ Total RNA Isolation Reagent (Welgene, Gyeongsan, Korea) according to the manufacturer's instructions. Total RNA was fragmented, mRNA was enriched or depleted, followed by reverse transcription and second strand synthesis. After adapter ligation and PCR amplification, ssCircs were converted into DNA nanoballs (DNBs) for PE100/PE150 sequencing.