title: HiC Library of Ajwa Date Palm
design: Chromatin was fixed in place with formaldehyde in nuclei and then extracted. Fixed chromatin was digested with DNAse I, chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter containing ends. After proximity ligation, crosslinks were reversed and the DNA purified. Purified DNA was treated to remove biotin that was not internal to ligated fragments. Sequencing libraries were generated using a NEBNext Ultra library preparation kit (New England Biolabs, Ipswich, Massachusetts, United States) and Illumina-compatible adapters. Biotin-containing fragments were isolated using streptavidin beads before PCR enrichment of each library.
library_name: Ajwa.DTG-OmniC-53