title: GSM9059226: Control Ly6Ghi CD101+ rep 3; Mus musculus; ATAC-seq
library_name: GSM9059226
library_construction_protocol: tdTOM+ neutrophil subpopulations (50000) were sorted from AML (Meis1-HoxA9 overexpression) mice and controls nad were washed once with cold phosphate-buffered saline (PBS) and resuspended with 50 µl cold ATAC-Resuspension Buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 3 mM Mg2Cl, 0.1% NP-40, 0.1% Tween-20, 0.01% Digitonin, and protease inhibitor). After incubation on ice for 3 minutes, the cell nuclei were collected by centrifugation at 500 g for 5 mins at 4°C. Tn5 transposition of nuclei pellets was carried out at 37°C for 30 min using the DNA Sample Preparation Kit from TruePrep Index Kit V2 for Illumina (Vazyme, TD501). The transposed DNA was purified using DNA purification columns (Zymo Research, D4003) and subjected to PCR amplification for library generation. ATAC-seq libraries were sequenced on the Illumina HiSeq 4000 platform for paired-end 150 cycles.