title: GSM9168283: Tet2 P53 tail control BF42; Mus musculus; OTHER
library_name: GSM9168283
library_construction_protocol: Genomic DNA was extracted using Qiagen Blood and Tissue Kit and Puregene Kits. After PicoGreen quantification, 100 ng of mouse genomic DNA were used for library construction using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR. After sample barcoding, 90-220 ng of each library were pooled and captured by hybridization with the mm_IMPACT (Nimblegen) (Integrated Mutation Profiling of Actionable Cancer Targets) assay, which captures all protein-coding exons and select introns of 578 cancer-related genes. Capture pools were sequenced on the HiSeq 4000 or NovaSeq 6000, using the HiSeq 3000/4000 SBS Kit or NovaSeq 6000 Reagent Kit (Illumina), respectively, for PE100 reads. Following these criteria, the average coverage was 361X for tumor samples and 191X for normals.