title: GSM9024980: BMDMs WT, Hepa1-6 CM treated, R2; Mus musculus; ATAC-seq
library_name: GSM9024980
library_construction_protocol: Density gradient centrifugation was performed to isolate cell nuclei. TruePrep DNA Library Prep Kit V2 for Illumina (TD501, Vazyme, Nanjing, China) was used to perform the transposition according to the manufacturer's recommendations. The transposed DNA fragments were purified by MinElute PCR Purification Kit (QIAGEN, Hilden, Germany). Library quality control was performed using the QSep-400 (BiOptic, Taipei, China) and Qubit 3.0 (Thermo Fisher Scientific, Wilmingto, USA). Sequencing was carried out on the Illumina Novaseq X platform (Illumina, San Diego, USA) with paired-end 150 bp (PE150) mode. Library construction and sequencing were performed at Biomarker Technologies (Hongkong) Co., Limited.