title: LdoS J-IP Agarose beads
design: DNA fragments containing base J were immunoprecipitated using antibodies against base J (PMCPMC3684241) and Protein A Agarose beads. After purification using AMPure XP beads, they were end-repaired and ligated with adapters before PCR amplification using NEBNext Ultra II Q5 High Fidelity DNA Polymerase.
library_name: JM085