title: RNA-seq of Human Fibroblast
design: Total RNA was isolated from cultured cells using standard column-based purification and quantified by spectrophotometry. To minimize contamination by genomic DNA, all RNA samples were treated with DNase prior to library construction. For cDNA synthesis, DNase-treated RNA was reverse transcribed using random hexamer primers and reverse transcriptase to generate cDNA.
library_name: A01