title: GSM9056155: MTA1-INPUT2; Homo sapiens; RIP-Seq
library_name: GSM9056155
library_construction_protocol: UV Crosslinking (254 nm) to covalently link RNA-binding proteins (RBPs) to RNA. Cell Lysis using NP-40/Triton-based buffer with protease/RNase inhibitors. RNA Fragmentation (optional, using RNase I) to generate ~50–100 nt RNA fragments. Immunoprecipitation (IP) with target-specific antibodies and Protein A/G beads. RNA Purification via protease digestion and phenol-chloroform extraction. RNA End Repair、Reverse Transcription (RT)、cDNA Purification & Amplification、Sequencing