title: GSM8511302: BJ8142,BJ8143,BJ8144, Mouse sperm, scHi-C; Mus musculus; Hi-C
library_name: GSM8511302
library_construction_protocol: All animal experimental procedures were carried out following the Institutional Animal Care and Use Committee (IACUC) guidelines of Peking University. 10–12-week-old C57BL/6J male mice were purchased from Peking University Laboratory Animal Centre. The single-cell preamplification product was diluted with water to 5 ng/uL. 2 μL of each diluted sample was transposed by the addition of 3 μL Transposition Mixture (1 μL 5 X TTBL, 0.25 μL TTE Mix V50 (Vazyme TD501), 1.75 μL water) and incubated at 55 °C for 10 min. Transposition was stopped by adding 1.25 μL 0.2% SDS and then the plate was incubated at room temperature for 10 min. Then, 2 μL 5 μM i5 unique dual (UD) index and 2 μ