title: sGff_culture_biol_rep1
design: sGff-IAEA-CC (~106 cells) at passage 12 were pelleted at 12,000g for 15 mins at 4C and resuspended in RNAlater. Total RNA was extracted from the PBS washed samples using the Total RNA Miniprep kit following standard protocols. Ribodepleted RNA-seq libraries were prepared by the Yale Center for Genomic Analysis and sequenced on the NovaSeq 6000 platform. Raw reads were quality filtered (Phred score >20 and read length >50 bp) and adapter-trimmed using fastp v0.24.0.
library_name: cult1