title: PacBio Revio sequencing of Pinus ponderosa var. ponderosa: buds have been set vegetative/pollen buds tissue (CBP02004C)
design: WGS PacBio analysis of normal buds have been set vegetative/pollen buds tissue (CBP02004C) (library ID F165874_tra75207, run ID 110659), constructed from sample accession SAMN53858304 for study accession PRJNA1187666. DNA extractions were performed at Bio S&T using the Bio S&T: Modified CTAB method was used to extract HMW DNA from ponderosa pine buds. Wide-bore pipette tips were used to reduce DNA shearing. Sequencing was performed at the Genome Sciences Centre (Vancouver, BC, Canada) using the PacBio Revio.
library_name: F165874_tra75207