title: RNA-Seq of Diadumene lineata: whole body after Agent #3 exposure
design: Total RNA was extracted from basal tissue of Diadumene lineata using phenol-chloroform. mRNA was enriched with oligo(dT) beads, fragmented, and reverse-transcribed into double-stranded cDNA using random hexamers. Libraries were prepared with the TruSeq RNA Sample Prep Kit, including end repair, A-tailing, adapter ligation, size selection (AMPure XP beads), and PCR enrichment. Final libraries were quantified by Qubit and qPCR, pooled equimolarly, and sequenced on Illumina NextSeq 550.
library_name: YA1_lib