title: 16S rRNA amplicon sequencing of Cervus canadensis: interdigital skin
design: Genomic DNA (gDNA) was extracted from tissue samples using the ZymoBIOMICS DNA Miniprep Kit, and cell pellets were subject to mechanical homogenization and DNA purification using the ZymoBIOMICS MagBead DNA/RNA Kit (Zymo Research). DNA samples were prepared for targeted sequencing using the Quick-16S Plus NGS Library Prep Kit and Primer Set targeting V3-V4. The final pooled library was cleaned up with the Select-a-Size DNA Clean & Concentrator. The final library was sequenced on Illumina NextSeq 2000 with a p1(Illumina, Sand Diego, CA) reagent kit (600 cycles). The sequencing was performed with 25% PhiX spike-in.
library_name: 22692_138