title: GSM9168076: A549 cells, VILMIR, 1 ng/mL IFN, rep2; Homo sapiens; RNA-Seq
library_name: GSM9168076
library_construction_protocol: Total RNA was isolated from cells following the TRIzol isolation method (Invitrogen). All samples were quantified and assayed to confirm minimum RNA integrity number of at least 9.7 using an Agilent TapeStation 4150. 500 ng of total RNA per sample underwent mRNA capture and was then fragmented at 94°C for 6 min. Sequencing libraries were prepared according to the manufacturer's protocol using eleven cycles of final amplification (KAPA mRNA HyperPrep Kit, catalog no. KK8580 and KAPA UDI Adapter Kit, catalog no. KK8727). Libraries underwent QC prior to sequencing using an Agilent TapeStation 4150.