title: GSM8758973: Human Retinoblastoma Tumor Replicate 2 - Sample_83268; Homo sapiens; RNA-Seq
library_name: GSM8758973
library_construction_protocol: For each specimen, 3–7 10μm formalin-fixed, paraffin-embedded sections were cut from a single representative block per case, using macrodissection with a scalpel as needed, to enrich tumor content. RNA was isolated using the Qiagen Allprep formalin-fixed, paraffin-embedded DNA/RNA kit (Qiagen, Valencia, CA) and quantified as previously described (PMID: 27102345). Library preparation and sequencing was performed using TruSeq® RNA Access Library Preparation Kit (Illumina) and Illumina HiSeq 4000 Libraries platform at University of Michigan Sequencing Core. Qiagen Allprep formalin-fixed, paraffin-embedded DNA/RNA kit (Qiagen, Valencia, CA)