title: 19Y000268
design: An overnight culture of E. coli #0960 and #0268 was prepared in LB containing 100 g/mL ampicillin and 50 g/mL kanamycin. A 1mL pellet was taken and DNA was extracted using ethanol precipitation and AMPure clean-up. Pellets were resuspended in nuclease-free water and transferred to a lysing matrix B tube (MP Biomedicals) and lysed using bead-beating (1min at 25Hz). Samples were centrifuged at high speed and sodium acetate (1/10 volume) and ice-cold ethanol (96-100%; volume equal to the total amount of supernatant) was added to the supernatant and incubated at -20C overnight. Samples were centrifuged at high speed and the pellet was washed twice with 70% ethanol, before being dried and resuspended in nuclease-free water. Samples were then mixed with AMPure XP beads (Beckman Coulter) and incubated at room temperature for 5 minutes. Using a magnet to avoid removing DNA bound to the AMPure beads, samples were washed twice with 70% ethanol. Following an air-drying step, nuclease-free water was added to the beads to resuspend DNA into the supernatant.Source Biosciences performed whole-genome Illumina sequencing on a NovaSeq 600 to generate 10M 150bp paired end reads.
library_name: 19Y000268