title: metagenome of Penares nux
design: Metagenomic DNA was extracted using the DNeasy PowerSoil Pro DNA Kit (Qiagen, USA). The V3-V4 region of the 16S rRNA gene was amplified using 341F and 805R primers with 2X sparQ HiFi PCR Master Mix (QuantaBio, USA). PCR conditions included an initial denaturation at 98C for 2 min, followed by 30 cycles of 98C for 20 s, 60C for 30 s, and 72C for 30 s, with a final extension at 72C for 1 min. Amplicons were purified using sparQ Puremag Beads (QuantaBio, USA) and indexed with Nextera XT index primers. The final library was pooled and diluted to 4 pM before sequencing. Paired-end (2  250 bp) sequencing was performed on an Illumina MiSeq at Omics Sciences and Bioinformatics Center, Chulalongkorn University, Thailand.
library_name: PF01_S64_L001_R1_001