title: 16S rRNA Amplicon Sequencing of Homo sapiens stool sample
design: Microbial DNA was extracted from stool samples using the QIAamp DNA Stool Mini Kit (Qiagen) following the manufacturers instructions. The stool microbiome was characterized by sequencing the variable region 3 and 4 amplicons of 16S rRNA gene according to the 16S metagenomic sequencing library protocol of Illumina. The library was denatured with NaOH, diluted, mixed with 5% PhiX control and finally loaded on MiSeq for high-throughput sequencing (2 x 300 cycle V3 kit).
library_name: CVID-1_Lib_17