title: 16S gene amplicon sequencing of DEP degrading microbial consortium
design: DNA was extracted from using the Macherey Nagel Nucleospin Microbial DNA kit. Extracted DNA was sent to BGI for sequencing. The V3-V4 hypervariable region was amplified using the 338F (ACTCCTACGGGAGGCAGCAG) and 806R (GGACTACHVGGGTWTCTAAT) primers. Sequencing on the DNBSEQ-G400 platform in the paired-end mode with a read length of 300 bp per read.
library_name: 34