title: GSM9077093: Sensitive 443; Homo sapiens; miRNA-Seq
library_name: GSM9077093
library_construction_protocol: Total RNA was extracted from microdissected chemo-refractory and chemo-sensitive HGSC tumor tissues using the RNAqueous-Micro kit (Thermo Fisher Scientific, Waltham, MA, USA), and the amount and quality of small RNA in the total RNA samples were determined using an Agilent small RNA kit (Agilent Technologies, Santa Clara, CA, USA) on an Agilent 2100 Bioanalyzer (Agilent Technologies). 5 ng of small RNAs were subjected to library construction using the Ion Total RNA-Seq kit v2 (Life Technologies Corp., Carlsbad, CA, USA), according to the manufacturer's small RNA protocol.