title: 16S rRNA gene V4V5 amplicon sequencing of Antarctic soils:HK_2
design: The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers (Caporaso et al. 2011). Each primer combination had a unique barcode corresponding to a unique sample. Barcodeded amplicons were normalized and pooled into a single library which was sequenced on a 2x150 Illumina MiSeq run. Sequences were were subsequently demultiplexed by barcode into individual files corresponding to each sample.
library_name: 2024_02_14_HK_2_16S