title: GSM8487846: RNA-control_rep4; Mus musculus; RNA-Seq
library_name: GSM8487846
library_construction_protocol: PicoPure™ RNA Isolation Kit was used to isolate the RNA from 3 pooled testis per replicate Two hundred and fifty ng of RNA were diluted to 50 uL in nuclease-free water and RNA libraries were prepared according to the TruSeq Stranded mRNA Library Prep protocol (Illumina, #20020594). HighPrep PCR beads were used for cleanup steps (MagBio Genomics, #AC-60050). SuperScript II Reverse Transcriptase was used for second strand synthesis (Invitrogen #100004925). cDNA was amplified with 14 PCR cycles.