title: Sleep deprivation disturbs diurnal rhythmicity of gut microbiota and inflammatory responses in mice
design: Sleep deprivation disturbs diurnal rhythmicity of gut microbiota and inflammatory responses in mice
library_name: Sample 33_p
library_construction_protocol: Control and Sleep deprivation groups of mouse ileum feces were harvested at time points ZT1,ZT5,ZT9,ZT13,ZT17 and ZT21. Mouse fecal bacterial DNA was extracted with Power Lyzer Power soil DNA isolation kit (catalog number: 12855-100, QIAGEN, Germantown, MD, USA)  Library was created by primers (341F/805R) designed to target the V3 and V4 regions of 16S rDNA, generate an amplicon about 465 bp in size.