title: DNA-Seq of Fritillaria biflora
design: For the DNA extraction, a modified CTAB protocol following Aboul-Ftooh et al., 2019 is implemented, utilizing a 2% CTAB solution with 2% PVP-40 in Fast Prep tubes containing Lysing Matrix A. During leaf sampling, 0.05g to 0.06g of leaf tissue, excluding the midrib for medium to large leaves, is macerated in a Fast Prep tube with 800ul of CTAB buffer. The Fast Prep homogenizer is employed with a 30-second cycle for tissue maceration.
library_name: GBI166_FB72