title: GSM8488300: fWT2A_H3K27ac; Mus musculus; ChIP-Seq
library_name: GSM8488300
library_construction_protocol: We used a native ChIP approach. For this 40-60 mg of frozen liver tissue was homogenized via a tissue grinder. After lysis, the nuclei pellet was digested with 20 U MNase for 15 min at 20 °C. Libraries from purified DNA were prepared using the NEBNext Ultra II DNA Library Prep kit following the manufacturer's instructions. After PCR enrichment of adaptor-ligated DNA, libraries were size selected at 150-700 bp in length using AMPure XP beads (Beckman Coulter) following the instructions of SPRIselect User Guide. Libraries were controlled via BioAnalyzer (Agilent) measurements using the High Sensitivity DNA