title: Adult non-failing heart 16: H3K27ac ChIPseq of human left atria cardiac myocyte nuclei
design: 5,000-10,000 sorted CM nuclei were used for ChIP-seq according to the manufacturer's protocol (Low Cell ChIP Kit, Active motif, 53086) using an antibody targeting H3K27ac (Active motif, 39133). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) according to manufacturers instructions. We monitored library amplification upon addition of EvaGreen using a qPCR cycler (Agilent). Amplification was stopped when the fluorescent signal reached the inflection point. We used Bowtie289 to assign the sequencing data to the corresponding position in the human genome hg19 in paired-end mode. Patient-sensitive genetic information was removed using BAMboozle (DOI:  10.1038/s41467-021-26152-8, hg19) using the parameter --strict.
library_name: LA-CM_9_ChIPseq_H3K27ac