title: GSM8574804: GL261_CC_hyp_rep2_RNAseq; Mus musculus; RNA-Seq
library_name: GSM8574804
library_construction_protocol: For RNAseq cells from hypoxia or normoxia conditions were fixed on ice with 3% Glyoxal fixation solution as was described previously (Channathodiyil, PLoS One. 2021). RNA was isolated using RNeasy Plus Kit (Qiagen) according to manufacturer's instructions and the quality was assessed with the Agilent 2100 Bioanalyzer with an RNA 6000 Pico Kit (Agilent Technologies). PolyA enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems). The RNAseq libraries were sequenced at 2x151 bp paired-end on NovaSeq 6000. Two biological replicates per condition were analysed.