title: GSM8861874: 5-PEO1-siNC-SP1-IP_ChIP; Homo sapiens; ChIP-Seq
library_name: GSM8861874
library_construction_protocol: 10 million cells were crosslinked with 1% formaldehyde for 10 min and quenched with 0.125 M glycine for 10 min. After cell lysis, chromatin was sonicated using a Covaris Ultrasonicator. ChIP DNA was purified using a ChIP DNA Clean & Concentrator kit (Zymo Research). ChIP DNA was used to prepare libraries for Next Generation Sequencing using the NEBNext Ultra II DNA kit (New England Biolabs, Ipswich, MA), according to manufacturer's directions. Overall library size was determined using the 4200 Tapestation and the High-sensitivity DNA 5000 assay (Agilent, Santa Clara, CA) and libraries were quantitated using the Qubit 2.0 Fluorometer (Thermofisher, Waltham, MA). Libraries were pooled and Next Generation Sequencing with a paired-end 2x60 bp run length was done on the Nextseq 2000 (Illumina, San Diego, CA).