title: GSM8686015: JMC200-2-5, rep 3; Candida albicans SC5314; ATAC-seq
library_name: GSM8686015
library_construction_protocol: ATAC-seq libraries were prepared from aliquots of 50,000 cells using the method described in Corces et al. Briefly, cells were incubated in hypotonic buffer in the presence of digitonin, washed, centrifuged to collect permeabilized samples, and resuspended in tagmentation buffer with Tn5 transposase (Illumina, San Diego, CA). Tagmented DNA was isolated with the Qiagen MinElute Reaction Cleanup kit and a dual-indexed library was prepared by subsequent PCR with Illumina-compatible primers and purified with AMPure XP beads (Beckman Coulter, Indianapolis, IN). Library quantity and quality were assessed by Qubit and Bioanalyzer (Agilent, Santa Clara, CA) assays, respectively, and paired-end sequencing performed on an Illumina NovaSeq 6000.