title: GSM8686043: 05_WT_HT1080; Homo sapiens; OTHER
library_name: GSM8686043
library_construction_protocol: Genomic DNA from cell cultures were purified after SDS and Pro-K mediated lysis, fragmented using a mix of restriction enzymes (HindIII, SspI, EcoRI, BsrGI and  XbaI), subjected to S9.6 antibody immunoprecipitation and sonication as described in Sanz et al., 2021, journal of Visualized Experiments. The kits used for the library prep were NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs, NEB, E6440) were used with 13 cycles of PCR amplification. The final 750 pM library was sequenced paired-end with dual index (60x8x8x60) using the Illumina NextSeq2000 P2 Reagents (100cycles) on the Illumina NextSeq2000 instrument. DNA: RNA Immunoprecipitation using S9.6 antibody followed by high-throughput sequencing (DRIP-seq)