title: GSM9021739: Lung__Pos_2; Mus musculus; RNA-Seq
library_name: GSM9021739
library_construction_protocol: After mRNA-S plus Ad5-S immunization, CD4+ T cells in the BAL, lung parenchyma, and draining lymph nodes in IL-21-VFP reporter mice were enriched with negative selection by using biotin-labeled CD8a, TER-119, and B220 antibody and streptavidin magnetic beads (Miltenyi Biotec) before surface markers staining. Live CD4+CD44+VFP+ and CD4+CD44+VFP- cells were sorted by Sony MA900 Cell Sorter and then resuspended in Trizol for RNA extraction. Two replicates of each cell subset were obtained. RNA quality was assessed and sequenced using Illumina 2 x 150 platform provided by Admera Health. cDNA libraries construction, sequencing alignment to the mouse reference genome through services were provided by Admera Health.