title: Long Reads for The Genomic Landscape of Head and Neck Cancer-associated Streptococci
design: Extracted DNA from all isolates was prepared for WGS using the MinION platform with R9.4.1 flow cells (Oxford Nanopore Technologies, Oxford, UK). Library preparation was performed using the Rapid Barcoding Kit 96 (SQK-RBK110.96; Oxford Nanopore Technologies Ltd.) according to the manufacturers instructions. DNA concentrations were measured using the Qubit 4 Fluorometer with the dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA). Base calling was conducted with Guppy (v6.2.11) in super accuracy mode using the dna_r9.4.1_450bps_sup.cfg configuration file.
library_name: ONT_8451DM9