title: GSM9106326: mutant cells, Day 31 of differentiation,  biol rep 4; Homo sapiens; RNA-Seq
library_name: GSM9106326
library_construction_protocol: Total RTotal RNA was extracted from 1.023, TMEM167A V59E.37 and V59E.48 iPSC-β cell differentiationstages S4, S5, S6, S7 and long-term culture (n=4 per stage and per iPSC line)using RNeasy Plus Micro Kit (Qiagen, Venlo, Netherlands), according to the manufacturer's recommendation. For the library preparation, all samples had at least 200 ng high-quality RNA with RNA integrity number (RIN) higher than 7, as calculated by Agilent Bioanalyzer. RNA libraries for RNA-seq were prepared using the Abclonal strand-specific mRNA library prep kit following manifacturer's protocol