title: GSM9075861: FLOX1; Mus musculus; RNA-Seq
library_name: GSM9075861
library_construction_protocol: Single nuclei were isolated from scWAT of GPSM1f/f (n = 3) and GPSM1ΔTreg (n = 3) mice using a sucrose gradient-based nuclei isolation protocol. Nuclei were stained with DAPI and sorted by fluorescence-activated cell sorting (FACS) to ensure high purity. Single-nucleus RNA-seq libraries were generated using the 10x Genomics Chromium Single Cell 3' Library, following the manufacturer's instructions. Briefly, GCs were resuspended in the master mikx andloaded together with partitioning oil and gel beads into the chip to generate the gelbead-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every singleGEM was retrotranscripted to cDNA, which contains an llumina R1 primer sequence.Unigue Molecular ldentifier (UMl) and the 10x Barcode. The pooled barcoded cDNAwas then cleaned up with Silane DynaBeads, amplified by PCR and the apropiatedsized fragments were selected with SPRlselect reagent for subsequent libraryconstruction. During the library construction llumina R2 primer sequence, paired-endconstructs with P5 and P7 sequences and a sample index were added.