title: GSM9385098: CUTRUN_H3K27ac_DMSO_treated_parental_H226_cells; Homo sapiens; OTHER
library_name: GSM9385098
library_construction_protocol: CUT&RUN profiling of H226 and H226-7883R cells was performed using the CUTANA ChIC/CUT&RUN Kit (Epicypher, #14-1048) following the manufacturer's instructions. Briefly, cells were prepared as single-cell suspensions, immobilized on activated ConA beads, and incubated with SNAP-Certified™ histone modification antibodies: H3K27ac (EpiCypher 13-0059, clone 2114-3E4) and H3K4me3 (EpiCypher 13-0060) at ~0.5 µg per reaction. After primary antibody binding, pAG-MNase was added and chromatin digestion activated with CaCl₂. Released DNA fragments were purified and quantified. Sequencing libraries were constructed using the CUTANA CUT&RUN Library Prep Kit (Epicypher, #14-1001/14-1002), with end repair, adapter ligation, and SPRIselect bead cleanups according to the manufacturer's protocol. Libraries were assessed on a TapeStation and sequenced as paired-end reads (~10 million PE reads per sample). Library preparation was conducted according to manufacturer's recommendations.