title: ITS amplicon seq of fungal: surrounding soil
design: DNA was extracted from 0.5 g of soil with the PowerSoil DNA Isolation kit (MoBio Laboratories, Carlsbad, CA, USA). After DNA concentrations were quality checked and quantified, all samples were stored at -80C until sequencing analysis. The library construction and sequencing of  ITS gene were performed1. The universal primer sets,  ITS1F and ITS2R for the fungal ITS1, were used in this study
library_name: Fm.RS.ITS.2