title: HTS of microbial communities on decaying fungal necromass and adjacent soil
design: To identify the bacteria present in soil and on fungal necromass, total genomic DNA was extracted using Qiagen DNEasy PowerSoil kits. The 16S rDNA subunit was PCR amplified using a barcoded bacteria-specific 515F-806R primer set under cycling conditions detailed in Caporaso et al. (2012). Amplified products were magnetically cleaned using the Agencourt AMPure XP kit (Beckman Coulter, Brea, CA, USA) and quantified using a Qubit dsDNA HS Fluorometer (Life Technologies, Carlsbad, CA, USA). Each of the samples were pooled into a single library and sequenced at the University of Minnesota Genomics Center using the 2 x 300 bp paired-end MiSeq Illumina platform.
library_name: 16S-68