title: GSM8861120: Telo-HUVECs, KRAS G12V, doxycycline induction, no treatment, replicate 2; Homo sapiens; RNA-Seq
library_name: GSM8861120
library_construction_protocol: RNA extraction was done using the TRIzol reagent and followed standard RNA extraction protocol. RNA samples (1μg/μL; 10μL) were submitted to Novogene Corporation Inc. for sequencing and downstream processing. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.