title: sequences of fungi
design: Universal primer sets with specific barcode were used to amplify the V3-V4 hypervariable region of bacterial and archaeal 16S rRNA genes, including 515F (5'-GTGYCAGCMGCCGCGGTA-3') and 909R (5'-CCCCGYCAATTCMTTTRAGT-3'). The ITS1 region of fungi was amplified by using the primers ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS3 (5'-GCTGCGTTCTTCATCGATGC-74').
library_name: G231_9d_4_fungi