title: RP-1240_Haq-RC0281-T-01_v3_Exome
design: Tumor-enriched tissue was dissected from FFPE tissue section slides, and concurrent DNA and RNA extraction was performed using a QIAGEN AllPrep FFPE DNA/RNA extraction kit. Library preparation and WES were performed for each sample. Peripheral lymphocytes were collected for a germline DNA sample. Hybridization and capture were performed with Illumina's Rapid Capture Exome Kit for WES and then sequenced with Illumina HiSeq as previously described36. Each run was a 76 bp paired-end, and Broad Picard Pipeline was used for de-multiplexing and data aggregation. WES from the newly sequenced cohort and previously published patients were aligned to hg19 to enable consistent downstream comparative analysis.
library_name: RP-1240_Haq-RC0281-T-01_v3_Exome_OnPrem