title: GSM8633718: Tg_c403_5_TransTag; Danio rerio; OTHER
library_name: GSM8633718
library_construction_protocol: 50 mL of alkaline lysis buffer (25mM NaOH, pH=12) were added to samples, and samples were heated at 95℃ for 30minutes. After brief vortex, 50 mL of neutralizing buffer (100mM Tris-HCl, pH=8) was added. Genomic DNAs were further purified using Zymo Research DNA Clean and Concentrator kit. About 100ng genomic DNAs were used as inputs to generate TransTag libraries to identify transgene insertion sites following the protocol provided in this manuscript