title: GSM9570518: Zebra finch embryo whole brain E14 heat call male E39; Taeniopygia guttata; Bisulfite-Seq
library_name: GSM9570518
library_construction_protocol: Whole brains were dissected from E14 embryos immediately after decapitation, embedded in OCT compound, flash-frozen on dry ice, and stored at −80°C. Genomic DNA was extracted from whole-brain tissue for RRBS library preparation. RRBS libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (NEB E7645S) and NEBNext Multiplex Oligos for Illumina (NEB E7355S) from 300 ng input genomic DNA per sample. Fragments were size-selected to ~250 bp, PCR-amplified for 9 cycles, and assessed by Qubit fluorometer and Agilent 2100 Bioanalyzer. Libraries were enriched for CpG-rich regions by MspI and TaqI digestion and sequenced on an Illumina HiSeq 2000 in 50 bp single-end mode.