title: WGS of Neobacillus driksii 179-J C42 HS
design: Samples were collected from the floor of the Spacecraft Assembly Facility at the Jet Propulsion Laboratory Samples were collected from 1 m2 of the floor using a 23x23 cm premoistened polyester wipe, placed in a glass bottle containing phosphate buffer saline, thoroughly mixed for 30 s, and concentrated using 0.45 m contracting pipette tip (InnovaPrep, Drexel, MO, USA). An aliquot of 425 L was heat shocked (80C, 15 min), and grown on tryptic soy agar incubated at 32C. Strain 179-C4-2-HS was restreaked from cryostock on TSA and genomic DNA isolated using a Zymo QuikSpin DNA extraction kit. Libraries for Nanopore sequencing were prepared using an SQK-LSK110 Ligation Sequencing kit (Oxford Nanopore) and sequenced on an R9.4.1 flow cell, with base calling via MinKNOW/Guppy.
library_name: 179-J C42 HS ONT