title: Genome sequencing of Oculina patagonica
design: Genomic DNA was isolated using two rounds of phenol-chloroform extraction from RNAse-A and proteinase K treated cell lysate. Sodium acetate (3 M, pH 5.2) was added to the final supernatant to a final concentration of 0.3 M, followed by the addition of 2 volumes of 100% ice-cold ethanol. The sample was incubated at -20C for 30 min to precipitate DNA. DNA was pelleted by centrifugation at 12,000 g for 20 min at 4C, washed with 1 ml of 70% ice-cold ethanol, and centrifuged again at 12,000 g for 10 min at 4C. After removing the ethanol, the DNA was dried and resuspended in 40 l EB buffer. Genomic DNA was quantified with a Qubit fluorometer and sequenced on an Oxford Nanopore using a PromethION flow cell (R9.4), generating 2.45 M reads with estimated N50 32.74 kb and 2M reads with N50 32.45 kb.
library_name: Ocupat_R1