title: GSM8686542: UCSD-AML1 DMSO 6hr rep2 [RNA-Seq]; Homo sapiens; RNA-Seq
library_name: GSM8686542
library_construction_protocol: Total RNA was extracted using the RNeasy Micro kit (QIAGEN, 74004) from 10,000-50,000 cells sorted or harvested in 25 µl Buffer RLT Plus with 1% BME. RNA libraries for RNA-seq were prepared using the SmartSeq2 protocol from the reverse transcription step using 10 ng of RNA. The whole transcriptome amplification step was set at ten cycles. Bulk RNA libraries were pooled at appropriate molar ratios to obtain at least 20 million reads per library. Libraries were subject to paired-end sequencing with 150 cycles.