title: GSM8547980: fetal liver, E14.5, BulkRNA-seq, RORgtmid_gtpos_Runx3pos_3; Mus musculus; RNA-Seq
library_name: GSM8547980
library_construction_protocol: Runx3-negative CLPs fractions and RORγt-mid fractions were each cultured on TSt4/DLL4 for 10-12 days. After collection by pipetting, the RORγt-positive cells with or without Runx3 expression were re-sorted respectively. Bulk RNA-seq was performed on 50-200 sorted cells using the SMART-Seq HT PLUS Kit following the manufacturer's instructions (Takara Bio).