title: RNA-Seq of Aurantiochytrium sp. PKU#Mn25: multiple factors based adaptive laboratory evolution
design: The total RNA extraction and transcriptome sequencing service was provided by Hangzhou Lianchuan Biotechnology Co. Ltd. The experimental process included library preparation and sequencing. The total RNA was extracted from frozen samples using an RNA kit (containing Trizol reagent). The quality and integrity of the extracted RNA were assessed using Nanodrop (Thermo Scientific? brand) and Agilent 2100 Bioanalyzer (Agilent Technologies, USA), while the RNA concentration was accurately determined using the Qubit RNA Assay Kit (Life Invitrogen, USA). For high-quality RNA samples, 2 g of total RNA was used to construct an RNA library, and deep sequencing analysis was performed on the Illumina HiSeq 2509 sequencing platform. Sequencing data were subjected to strict quality control, including removal of repetitive sequences, adapter sequences, and low-quality reads.
library_name: M4C_48_1