title: GSM9168253: female T cells; Mus musculus; RNA-Seq
library_name: GSM9168253
library_construction_protocol: Tumors were digested at 37°C using type IV collagenase (Stemcell Technologies, catalog number 07427), red blood cells were lysed using RBC lysis buffer (Thermo-Fisher Scientific, catalog number 00-4333-57), and homogenates were passed through 100-micron strainers to obtain a single cell suspension. Viable CD4+ T cells were isolated by fluorescence-activated cell sorting (FACS). Single cell RNA libraries from 10,000-20,000 cells per sample were generated using a Chromium Controller and the GEM-X Single Cell 5' kit (catalog number 1000707) from 10X Genomics. Library sequencing was performed by the Genome Technology Access Center at Washington University in St. Louis.