title: 16S rRNA gene amplicon sequencing of soil bacteria (E treatment, Rep4)
design: Total genomic DNA was extracted from soil samples. The V3-V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers F: ACTCCTACGGGAGGCAGCA and R: GGACTACHVGGGTWTCTA (process version: v3.2). Amplicons were purified and used to construct an Illumina sequencing library, which was sequenced with paired-end reads.
library_name: Bacteria_E4_raw_lib