title: mountain wetland rrna seqs
design: Total DNA was isolated from 0.5 g of soil samples using DNeasy PowerSoil Kit according to the manufacturers instructions. The V3V4 variable region of the prokaryotic 16S rRNA genes was obtained by PCR with primers 341F and 806R. PCR fragments were barcoded using Nextera XT Index Kit v2
library_name: T132