title: GSM9056533: Wild-type mouse kidney control WT1; Mus musculus; RNA-Seq
library_name: GSM9056533
library_construction_protocol: Total RNA was extracted using the miRNeasy RNA purification kit (Qiagen) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.