title: GSM8893216: Methylobacterium extorquens SLI 505 in 50 mM methanol, biological replicate C; Methylorubrum extorquens; RNA-Seq
library_name: GSM8893216
library_construction_protocol: 40 mL of culture were harvested at mid-exponential phase (OD600 of 0.5-1 for all conditions) by centrifugation at 4°C at 2000 x g for 10 minutes. Total RNA was extracted using the Qiagen RNeasy Kit and quantified. rRNA depletion using the Ribo-Zero RNA Plus rRNA Depletion Kit (Illumina) was performed by SeqCoast Genomics. Library preparation and Illumina Hi-Seq sequencing was performed by SeqCoast Genomics.