title: QIC-seq of Huh7.5.1 cells challenged with DENV, WNV, YFV, and LGTV. QIC-seq of Hap1 cells challenged with DENV and WNV
design: QIC-seq of Huh7.5.1 cells challenged with DENV, WNV, YFV, and LGTV. QIC-seq of Hap1 cells challenged with DENV and WNV
library_name: Sample 9_p
library_construction_protocol: After 48 hours of mock or infection, cells were washed, and trypsinized and resuspended in PBS and BSA to prevent clumping.  Cells were grown in complete DMEM or IMDM media supplemented with 10% FBS.  Cells were either mock infected or infected with virus at varying MOIs for 48 hours.  Cells were lysed, and RT-PCR was used to capture host transcriptome, viral genomes, and sgRNAs to identify perturbed cells. cDNA was size selected to generate a larger cDNA library (transcriptomic) and a smaller DNA library (viral and CRISPR) .  10X genomics 5' VDJ protocol was followed for the transcriptomic library, READ2 was placed onto cDNA in small viral and CRISPR library by PCR before re-incorporation into the remaining protocol. 