title: GSM9059485: DLD1_PP10; Homo sapiens; RNA-Seq
library_name: GSM9059485
library_construction_protocol: RNA was extracted by using Trizol Total RNA was used as input material for the RNA sample preparations.  Briefly, mRNA was purified from total RNA by using poly-T oligo-attached  magnetic beads. Fragmentation was carried out using divalent cations under  elevated temperature in First Strand Synthesis Reaction Buffer(5X). First strand  cDNA was synthesized using random hexamer primer and M- MuLV Reverse  Transcriptase, then use RNaseH to degrade the RNA. Second strand cDNA  synthesis was subsequently performed using DNA Polymerase I and dNTP.  Remaining  overhangs  exonuclease/polymerase  were  activities.  converted  After  into  blunt  adenylation  ends  via  of 3' ends  of DNA fragments, Adaptor with hairpin loop structure were  ligated  to  prepare  for hybridization. In  order to select cDNA  fragments of preferentially 370~420 bp in length, the library fragments  were purified with AMPure XP system (BeckmanCoulter,Beverly, USA).  Then PCR amplification, the PCR product was purified by AMPure XP beads, and  the library was finally obtained