title: GSM9058387: PODO/TERT256 human podocytes, fractal topography, cytokines, rep 1; Homo sapiens; RNA-Seq
library_name: GSM9058387
library_construction_protocol: RNA was extracted using the PicoPure RNA extraction and isolation kit (Applied Biosystems). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non strand specific library. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on the Illumina NovaSeq X Plus platform with a read length of 2x150 paired-end reads to a depth of 20 million reads in each direction.