title: GSM9351392: Rat facial nerve tissue following injury  Model1; Rattus norvegicus; RNA-Seq
library_name: GSM9351392
library_construction_protocol: Total RNA was extracted from samples using TRIzol reagent. RNA concentration was measured using NanoDrop. The integrity of RNA was assessed by agarose gel electrophoresis. Sequencing libraries were constructed following the standard Illumina protocol. Briefly, mRNA was enriched from total RNA using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into double-stranded cDNA. After end repair, A-tailing, and adapter ligation, the cDNA fragments were PCR-amplified. Final library quality and size distribution were validated using Qubit and an Agilent Fragment Analyzer.