title: sequences of gene
design: Samples were sequenced and analyzed by Shanghai Personal Biotechnology Cp. Ltd. Briefly, sequencing libraries were constructed by TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), quantified using qRTPCR and the Agilent high sensitivity DNA assay on Bioanalyzer 2100 system (Agilent), and then sequenced on Illumina Novaseq 6000 system to generate 150bp pair-end reads. The reads, filtered by FastQC, were mapped to human genome (hg38) using HISAT2 software. Read counts were calculated using HTSeq v0.9.1 and output as FPKM upon normalization.
library_name: Normal-2