title: Donor T2 + ILC2
design: T cells were cultured alone or with ILCs at 2:1 ratio in a trans-well system with CD3/28 activation beads for 24 hours. Cells were debeaded and further cultured for a total of 3 days. Some conditions received anti-IL-9 blocking antibody. At Day 3 RNA was extracted. 500ng of RNA was used to prepare Illumina HiSeq libraries according to manufacturer's instructions (TruSeq Stranded RNA kit) and sequenced as single pass 50bp reads on the Illumina HiSeq4000.
library_name: T22