title: R4D4_2
design: The quality of the isolated RNA was determined with a Fragment Analyzer (Agilent, Santa Clara, California, USA). The Stranded Total RNA Prep with Ribo-Zero Plus (Illumina, Inc, California, USA) was used in the succeeding steps. Briefly, total RNA samples (100-1000 ng) were depleted with ribosomal RNA and then reverse-transcribed into double-stranded cDNA. The cDNA samples were fragmented, end-repaired and adenylated before ligation of TruSeq adapters containing unique dual indices (UDI) for multiplexing. Fragments containing TruSeq adapters on both ends were selectively enriched with PCR.  The quality and quantity of the enriched libraries were validated using a Fragment Analyzer (Agilent, Santa Clara, California, USA).  The product was a smear with an average fragment size of approximately 260 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.
library_name: R4D4_2