title: Multi-omics profiling of jejunum and duodenum in Longyan Shan Partridge ducks with divergent residual feed intake SN_20
design: Total genomic DNA was extracted from the samples using the E.Z.N.A. Soil DNA Kit. The quality, concentration, and purity of the extracted DNA were assessed via agarose gel electrophoresis and a NanoDrop2000 spectrophotometer. The V3-V4 hypervariable region of the 16S rRNA gene was amplified via PCR using the 338F/806R primer set. The PCR products were purified, and sequencing libraries were constructed using the NEXTFLEX Rapid DNA-Seq Kit. Sequencing was performed on an Illumina Nextseq2000 platform.
library_name: SN_20