title: Genome-wide methylation drift and transcriptomic variability in aged beagle dogs - RNA-seq
design: Genome-wide methylation drift and transcriptomic variability in aged beagle dogs - RNA-seq
library_name: JB-b11-1_p
library_construction_protocol: A total of 24 beagle dogs aged 3 years (n = 8), 5 years (n = 8), and 10 years (n = 8) were investigated for age-related molecular signatures in their methylomes. All dogs were kept at the NIAS under standardized housing conditions that were maintained through continuous monitoring, vaccination, and anthelmintic treatment. The experiment was ethically approved by the Institutional Animal Care and Use Committee of the National Institute of Animal Science (NIAS 2022-586) and Gyeongsang National University (GNU-240802-D0152-01). Whole blood samples were collected using a 21-gauge needle and 5-mL syringe from the jugular vein of each dog. RNA from whole blood was extracted using a PAXgene Blood RNA Kit (762174; QIAGEN, Germantown, MD, USA) following the manufacturerâ€™s instructions.  Libraries were prepared for 150 bp paired-end sequencing using a TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA).