title: PacBio HiFi sequencing data for Helminthoglypta traskii
design: The HiFi SMRTbell library was constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences, Menlo Park, CA; Cat. #100-938-900) according to the manufacturer's instructions. HMW gDNA was sheared to a target DNA size distribution between 15 kb  18 kb using Diagenodes Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated using 0.45X of AMPure PB beads (Pacific Biosciences, Menlo Park, CA; Cat. #100-265-900) for the removal of single-strand overhangs at 37C for 15 minutes, followed by further enzymatic steps of DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 10 minutes and 65C for 30 minutes, and ligation of overhang adapters v3 at 20C for 60 minutes. The SMRTbell library was purified and concentrated with 1X AMPure PB beads for nuclease treatment at 37C for 30 minutes and size selection using the BluePippin/PippinHT system (Sage Science, Beverly, MA; Cat #BLF7510/HPE7510) to collect fragments greater than 7-9 kb. The 15  20 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using three 8M SMRT cells, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel IIe sequencer.
library_name: CCGP_gDNA0101