title: GSM8720705: E2F1 WT, SETD6 WT, Replicate 2; Homo sapiens; RNA-Seq
library_name: GSM8720705
library_construction_protocol: For the DU145 CRISPR/Cas9 generated SETD6 knock-out, single clones were confirmed by sequencing as described in previously (Kublanovsky et al., 2023) total RNA was isolated from approximately 1 × 106 cells by using the RNeasy® Mini Kit (Qiagen). The extracted RNA was quantified by NanoDrop1000 and reverse-transcribed into complementary DNA (cDNA) to generate RNA-seq libraries.