title: GSM9167965: P37_BrM_ROI_4_Protein; Homo sapiens; OTHER
library_name: GSM9167965
library_construction_protocol: ROIs (300 µm) from high- and low-TIL tumor regions (92 glioma, 90 brain metastases) were UV-cleaved after staining with Alexa Fluor 488–SYTO 13 (nuclei) and Alexa Fluor 594–anti-CD3 (TILs). Released RNA and protein oligos from the same ROIs were collected from FFPE tissue and used for library preparation. RNA libraries: GeoMx Whole Transcriptome Atlas (~18,000 genes) + custom pan-microbial probe panel (incuding exploratory probes not tested in this study). Protein libraries: Immuno-Oncology Protein Atlas (>570 targets). Indexed per manufacturer's Spatial Proteogenomic Library Prep Protocol. Libraries were pooled at a 2:1 (RNA:protein) molar ratio and sequenced on Illumina NovaSeq 6000 S2 (paired-end, 2 × 27 bp, i5/i7 dual indexing).