title: 16S rRNA gene sequencing of swine jejunum mucosa
design: Swabs were transported back to the lab and vortexed vigorously for 30 seconds in PBS . A 0.25 mL aliquot of the resulting PBS solution was used for DNA extraction with the ZymoBIOMICS 96 MagBead DNA Kit . The V4 region of the 16S rRNA gene was amplified and sequenced using the dual index approach described in Kozich et al. with the Accuprime Pfx Supermix. The pooled and normalized libraries were sequenced on the Illumina Miseq (MiSeq Reagent Kit V2 500 cycles).
library_name: JS89