title: GSM9021235: IL-33 prime, rep 2; Mus musculus; ATAC-seq
library_name: GSM9021235
library_construction_protocol: 50,000 live PCMCs were lysed and processed to isolate nuclei, followed by transposition with Tn5 transposase at 37 °C for 30 minutes. Equimolar adaptors were ligated, and libraries were amplified by PCR using the ATAC-seq_Novogene Kit (Novogene). Libraries were purified with AMPure beads and quantified using Qubit. Sequencing was performed on the Illumina NovaSeq platform generating 150bp paired-end reads.