title: Hippocampal Transcriptomic Signatures of Stress Resilience and Susceptibility in the CSDS Mouse Model 7 hours after the last stress
design: Hippocampal Transcriptomic Signatures of Stress Resilience and Susceptibility in the CSDS Mouse Model 7 hours after the last stress
library_name: 8_p
library_construction_protocol: Defeated and control animals underwent a final defeat session and were euthanized by decapitation 7 hours after sensory interaction to preserve brain tissue integrity. Hippocampi were dissected and snap-frozen in liquid nitrogen Total RNA was extracted from hippocampal extract with TRIzol Reagent (Invitrogen) following manufacturer protocol and resuspended in DEPC water. RNA concentration and quality was assessed by 4200 Tape Station (Agilent).  Universal Plus mRNA-Seq kit (Tecan Genomics, Redwood City, CA) has been used for library preparation following the manufacturerâ€™s instructions (library type: fr-secondstrand). RNA samples were quantified and quality tested by Agilent 2100 Bioanalyzer RNA assay (Agilent technologies, Santa Clara, CA) or Caliper (PerkinElmer, Waltham, MA). Final libraries were checked with both Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) and Agilent Bioanalyzer DNA assay or Caliper (PerkinElmer, Waltham, MA).