title: GSM9165718: ICR 4-cell embryos, 0.1 mg/mL Nanoplastic treated, replication 2; Mus musculus; RNA-Seq
library_name: GSM9165718
library_construction_protocol: Total RNA was extracted from pools of 40 embryos per group using Rneasy Micro Kit ((Qiagen) following the manufacturer's protocol. RNA integrity was assessed using an Agilent 4200 TapeStation. Total RNA was used to construct sequencing libraries with the Discover-sc WTA Kit V2 (Vazyme, N711) and TruePrep Flexible DNA Library Prep Kit for Illumina (Vazyme, TD504) following the manufacturer's instructions. Briefly, cDNA was synthesized via reverse transcription, amplified, fragmented, and ligated with sequencing adapters. Index PCR was performed using the TruePrep Index Kit V2 for Illumina (Vazyme, TD202). Final libraries were quantified using qPCR and assessed with Agilent 4200 TapeStation. Sequencing was performed on the NovaSeq 6000 platform (Illumina).