title: GSM9057931: KOLF2.2J cells, OsTIR1(F74G), parent, biol rep1; Homo sapiens; RNA-Seq
library_name: GSM9057931
library_construction_protocol: Total RNA was isolated using the Quick RNA miniprep kit (Zymo Research, R1057) with DNase I treatment by following the manufacturer's instructions. The integrity of total RNA was assessed through 1% TAE agarose gel electrophoresis. mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L). RNA-Seq libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs, E7760L) and NEBNext Multiplex Oligos (NEB, E7500L) with index primers according to the manufacturer's instructions.