title: GSM8966506: LNCaP, R1881, pGIPZ, GATA2 ChIP; Homo sapiens; ChIP-Seq
library_name: GSM8966506
library_construction_protocol: PCa cells were crosslinked with 1% formaldehyde for 10 min at room temperature with gentle rotation and then quenched for 5 min with 0.125 M glycine. Chromatin was sonicated to an average length of 200–600 bp using an E220 focused-ultrasonicator (Covaris). ChIP–seq libraries were prepared from 3–5 ng of ChIPed DNA using NEBNext Ultra II DNA Library Prep Kit, according to the manufacturer's instructions.