title: DNA sequencing of HT-PAMDA library for SSM_39 enzyme on Plate2 at 32 min
design: HT-PAMDA was used to measure Cas9 PAM preferences by incubating Cas9gRNA complexes with a plasmid library encoding all possible PAMs adjacent to a fixed protospacer. PAM-specific cleavage rates were inferred from time-resolved depletion of sequences measured by Illumina amplicon sequencing.
library_name: SSM_39_Plate2_32min_CCAAG