title: DNA-Seq of novel lytic phage
design: High quality genomic DNA (gDNA) was isolated from the received culture using QIAamp DNA mini kit (Qiagen). gDNA that passed the preliminary agarose gel inspection was further subjected to purity check spectrophotometrically to measure the A260/280 ratio and its concentration was measured using Qubit broad range dsDNA quantification kit (Thermo Fisher Scientific). 250ng of total DNA was used as input for library preparation using QIASeq FX DNA kit (Qiagen) to fragment and obtain adapter ligated and indexed library as per manufacturers instructions. The indexed library was sequenced on an Illumina NextSeq 2000 using a 300 cycle paired end chemistry.
library_name: RJ_SW_PA_24_12_N3