title: Bacillus thuringiensis
design: WGS of bacterial isolate. Genomic DNA extraction was performed according to the GeneJet Genomic DNA purification kits protocol. The concentration and quality of extracted DNA was determined using Nanodrop Lite spectrophotometer (Thermo Scientific, Wilmington, DE, USA), Qubit dsDNA BR assay (Thermo Fisher Scientific, Wilmington, DE, USA) and agarose gel electrophoresis. Illumina libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (NEB, USA) and sequenced on the NovaSeq 6000 platform using the 2  150 bp paired-end read strategy.
library_name: CRE1-Illumina