title: GSM8861337: KOStra8 mouse 3; Mus musculus; RNA-Seq
library_name: GSM8861337
library_construction_protocol: Total RNA was extracted from the testis using RNA-Stat 60 (IsoTex Diagnostics), chloroform, followed by purification using a total RNA extraction kit (Omega Bio-Tek-R6834-02) with DNaseI digestion (Roche). RNA quality and concentration were examined using the Agilent tapeStation and AccuBlue Broad Range RNA Quantitation assay, respectively. Library constructions were done according to the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc., Massachusetts, USA). Final libraries were assessed for quantity and quality with an average size of 430bp. Equimolar pooling of libraries was performed based on QC values and sequenced on an Illumina Novaseq platform with 150PE reads (Illumina, California, USA).