title: GSM9058495: RBNS_ZC3H4_WDR82_1200nM_rep2; synthetic construct; OTHER
library_name: GSM9058495
library_construction_protocol: RNAs with a random region of 40nt were prepared by in vitro transcription and incubated with an increasing concentration of the three zinc fingers motif or ZC3H4-WDR82 omplex (0, 5, 20, 80, 320, 1200, 2400 and 6000 nM). Complexes were eluted from the beads by incubation in 50 μL elution buffer (10 mM Tris-HCl pH 7.0, 1mM EDTA, 1% SDS) at 70°C for 10 min. in agitation at 1200 rpm in a Thermomixer. RNA was extracted using the Trizol LS Reagent following the manufacturet's instruction The extracted RNA was reverse transcribed into cDNA. Illumina sequencing libraries were generated by amplifying the resulting cDNA via PCR with Phusion DNA Polymerase (NEB M0530L). Primers containing Illumina adapters and a single index were used for this amplification.