title: ChIP-seq of potato: leaves 9h dark 2
design: eaves of 2-week-old StBBX20-mGFP-His transgenic line collected upon 6 hours of light and 9 hours were cross-linked in 1% formaldehyde under a vacuum for 25 min. ~2.5 g of cross-linked leaves were ground to a powder in liquid nitrogen and subjected to nuclei isolation. Sonicated chromatin was used for immunoprecipitation with polyclonal anti-GFP(FL) antibody (Santa Cruz Biotechnology, Inc.) The immunoprecipitated protein-DNA complex was then purified using protein A agarose beads. 5-10 ng CHIP-enriched, fragmented DNA was used for library preparation using the TruSeq ChIP Library Preparation Kit (Illumina).
library_name: CHIP_BBX20_D2