title: GSM8760131: RNA sequencing from gastruloids embedded at 72h in 1mM Dextran hydrogel replicate 1; Mus musculus; RNA-Seq
library_name: GSM8760131
library_construction_protocol: Gastruloids in 96-well plates (control and negative control) were collected into falcon tubes. All gastruloids were then washed twice with cold PBS (Ca++/Mg++) before being snap-freezed in liquid nitrogen, and stored at -80°C until RNA extraction. RNeasy Mini kit (Qiagen) with on-column DNase digestion was used for RNA extraction following manufacturer's instructions. RNA quality was assessed on a TapeStation TS4200, all RNA samples showed quality number (RIN) above 9. RNAseq library preparation with Poly-A selection was performed with 550 ng of RNA using the Illumina stranded mRNA ligation and following the manufacturer's protocol 1000000124518 v01. Libraries were quantified by qubit DNA HS and profile analysis was done on TapeStation TS4200. Libraries were sequenced on Novaseq 6000, with paired end 75 bp reads.