title: Lamin-B1 CUT&Tag of Emu: adult female left brain
design: The CUT&Tag experiments were performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, TD-903) following the manufacturer's instructions. In brief, dispersed cells were attached to paramagnetic concanavalin A (ConA) beads and incubated with Anti-Lamin B1 antibody (Abcam, ab16048) on a rotator at 4C overnight. Subsequently, the supernatant was incubated with highly active pA/G-Tn5 transposases at room temperature for 1 hour and followed by fragmentation at 37C for an additional hour. Libraries were prepared using the fragmented DNA and indexed with the TruePrep Index Kit for Illumina DNA (Vazyme, TD-202). Sequencing was then performed via paired-end 150 bp reads on the NovaSeq 2500 platform (Illumina, China, Novogene).
library_name: Emu_F_L_B_LMNB1