title: GSM8686103: TOP3BKO_HT1080_S4; Homo sapiens; RNA-Seq
library_name: GSM8686103
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Invitrogen), followed by further purification using PureLink RNA Mini Kit (Cat No. 12183018A;Invitrogen) with DNase treatment (QIAGEN). RNA quality and quantity were analyzed with 2100 Expert (Agilent). RIN (the RNA Integrity Number) of all samples were above 8.0. NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) were used. NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, NEB, E6440) were used with 13 cycles of PCR amplification. The final 750 pM library was sequenced paired-end with dual index (101x8x8x101) using the Illumina NextSeq2000 P2 Reagents (200cycles) on the Illumina NextSeq2000 instrument. The total number of reads was 550M (see below for individual sample).