title: Bulk K562 and EL4 cells mixed with 12 Time-Tags for verification (QC) for ChronoSeq Beads
design: Bulk K562 and EL4 cells mixed with 12 Time-Tags for verification (QC) for ChronoSeq Beads
library_name: BULK_QC_12TP_K562_EL4_p
library_construction_protocol: Cells were manually mixed directly with the beads. 10μl of Time-Tagged beads at 450 beads/μl suspended in lysis buffer were directly mixed with 8μl of mouse or human cells at 600cells/μl. Odd numbered Time-Tags were mixed with human(K562) cells while even numbered Time-Tags were mixed with mouse(EL4) cells. K562 and EL4 cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. Beads are washed once with 6X SSC buffer and then twice with 1.25X RT buffer. Beads were then pooled together for a single RT reaction. very similar to Drop-seq. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html