title: GSM9235552: 25c_ERC_SVB; Homo sapiens; RNA-Seq
library_name: GSM9235552
library_construction_protocol: Entorhinal cortex was dissected from fresh frozen postmortem human tissue, 100 micron sections were obtained via cryo sectioning. Frozen cryosections were homogenized in chilled Nuclei EZ Lysis Buffer, then filtered and centrifuged to isolate nuclei. Pellets were washed in PBS containing 1% BSA and RNase Inhibitor, stained with propidium iodide, and passed through 35 μm strainers. Fluorescence-activated nuclear sorting (FANS) was performed on a MoFlo Legacy Cell Sorter to select whole, singlet, G0/G1 nuclei. Library preparation for Chromium Next Gem Single Cell 3' v3.1 was completed according to the manufacturer's protocol.