title: 16S rRNA Limulus
design: The samples were prepared for sequencing using custom target primer sets (V3-V4) of the Quick-16S NGS Library Prep Kit (Zymo Research, United States). The library was sequenced on Illumina MiSeq with a v3 reagent kit (600 cycles) to generate paired-end reads of ~ 400 base pairs (bp). Sequencing was performed with > 10% PhiX spike-in by the ZymoBIOMICS Service (Zymo Research, United States).
library_name: 6V3V4