title: RNA-Seq of Zebrafish embryos (Ctrl)
design: Total RNA was extracted from zebrafish embryos microinjecting of empty plasmid. Library preparations were conducted using a mix of 250ng total RNA and 0.25 ng Spike-in RNA Variant Control Mix E2 (Lexogen, Austria) with the TruSeq RNA Library Prep Kit (Illumina). The ready-to-sequence libraries werequantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries(Kapa Biosystems, USA), and The ready-to-sequence libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (Kapa Biosystems, USA), and library profiles were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA).
library_name: ZF_Ctrl_4