title: SC-HTO seq of mus musculus: adult female thymus
design: Thymocyte suspension from WT and CD3eI173A were stained with different TotalSeq-C0304, -C0305 and -C0306 anti-mouse Hashtag 4/5/6/ Antibody hashtag reagents, then following stained with CD4, CD8 fluorescent antibodies and Live/Dead dye with recommended concentrations at 4 for 30 min. Then sorted CD4 SP (CD4+CD8-), CD8 SP (CD4-CD8+) and DP (CD4+CD8+) T cells by flow cytometry. The CD4 SP T cells from WT and CD3eI173A were mixed for single-cell sequencing.
library_name: 2210WFLW1_1HTO