title: Transcriptome analysis of rattus norvegicus fed cafeteria diet: adult female brain
design: Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Kit (Illumina) from rat brain total RNA extracted with the Direct-Zol Mini Prep Kit. Libraries underwent adapter ligation, reverse transcription, PCR amplification, and AMPure XP bead-based size selection. Quality was assessed using the Agilent 4200 TapeStation, and sequencing was performed on the Illumina NextSeq500 (175 bp, ~10M reads/sample).
library_name: CafD