title: 16S rRNA amplicon sequencing of the snout and cloacal microbiota of Crocodylus intermedius
design: DNA extraction was performed as described in Rudolf et al., (2018). The 16S rRNA gene was amplified using universal V4 primers 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 805R (5-GGACTACHVGGGTWTCTAAT-3) following protocols from the Earth Microbiome Project (https://earthmicrobiome.org/protocols-and-standards/). Sequencing was performed on an Illumina MiSeq platform.
library_name: B52