title: ddRAD-seq of Chorthippis grasshoppers
design: 400ng DNA digested using enzymes EcoRI-Hf & MseI. Followed by adapter ligation for individual barcoding. Samples were pooled into libraries (LX) and size-selected for fragments between 300-400bp using a Blue Pippin. Libraries were amplified with Phusion HF DNA polymerase for 15 cycles in 7 parallel reactions that were then combined (7 parallel reactions per library were combined). Libraries were then purified with CleanNGS magnetic beads and sequenced by Biomarker Technologies (BMK) on a Novaseq6000. Libraries were sequenced for 150bp paired end reads with an average of 3.2M reads per individual.
library_name: RP1493_L3