title: 16S rRNA gene V4V5 amplicon sequencing of hot spring incubation of plastic: Tikitere PHB Biofilm
design: The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers (Caporaso et al. 2011). Each primer combination had a unique barcode corresponding to a unique sample. Barcoded amplicons were normalized and pooled into a single library which was sequenced on a 2x250 Illumina MiSeq run. Sequences were subsequently demultiplexed by barcode into individual files corresponding to each sample.
library_name: 48exp9PHB