title: GSM8759878: Plasmodium falciparum treated with 0mM GlcN (repeat 1); Plasmodium falciparum 3D7; RNA-Seq
library_name: GSM8759878
library_construction_protocol: PfAP2-V parasites in the ring, trophozoite, and schizont stages were treated for one cycle in 5 mM GlcN . Parasite survival was monitored using Hoechst 33342 (Beyotime, #C1027, China) according to the manufacturer's instructions, and all pure iRBCs were obtained by sorting using an Aria III flow cytometer (BD Biosciences, USA). Two single-cell libraries were obtained using the protocol provided with the Chromium™ Single Cell 3 Reagent Kit (V3 Chemistry). The final library pool was sequenced on an Illumina NovaSeq 6000 instrument using 150-base-pair paired-end reads.