title: GSM9166696: MacacaPFCsnRNA_P0_1_2; Macaca fascicularis; RNA-Seq
library_name: GSM9166696
library_construction_protocol: Macaque prefrontal cortex samples across developmental stages were collected in ice-cold oxygenated artificial cerebrospinal fluid at PH 7.4.  Samples were transferred into 2 ml ice-cold EZ PREP and were homogenized by grinding with a glass Dounce tissue grinder . Transfer the tissue homogenate into a 15 ml tube, add 2 ml of EZ PREP, incubate on ice for 5 minutes and centrifuge at 500 g at 4℃ for 5 minutes. The supernatant was removed and the nuclei were resuspended in 4 ml ice-cold EZ PREP, and the incubation and centrifugation were repeated once. The libraries were generated by using the Chromium Next GEM Single Cell 3' Kit v3 according to the 10x GENOMICS official instructions, and processed on the Illumina platform for sequencing with 150 bp paired-end reads