title: K562+EL4 (Time Tag 10), K562(Time Tag 11), EL4 (Time Tag 12) (QC) 215 cells/ μl 3 Time-Tags. ChronoSeq No Stimulus. Only Checking Time-Tags with a Sampling Interval of 7minutes
design: K562+EL4 (Time Tag 10), K562(Time Tag 11), EL4 (Time Tag 12) (QC) 215 cells/ μl 3 Time-Tags. ChronoSeq No Stimulus. Only Checking Time-Tags with a Sampling Interval of 7minutes
library_name: SC_3TP_QC_K562_EL4_p
library_construction_protocol: K562+EL4 (Time Tag 10), K562(Time Tag 11), EL4 (Time Tag 12). Automated cell sampling using ChronoSeq device. Each cell sample is injected with a unique injection of Time-Tagged ChronoSeq beads. https://kanishkasthana.github.io/ChronoSeq/protocol_and_software_for_running_chronoseq_device.html K562 and EL4 cells grown in suspension in DMEM with 10% FBS and 1%PEN-STREP at 5%CO2 and 37C.  https://kanishkasthana.github.io/ChronoSeq/protocol_for_preparing_cells_AdditionalFiltration.html Oligo-DT beads were used to capture mRNA from lysed Single-Cells inside droplets. ChronoSeq library preparation protocol is very similar to Drop-seq. Beads from all time-tags are pooled together for Reverse Transcription followed by PCR amplification. https://kanishkasthana.github.io/ChronoSeq/protocol_library_preparation_for_dropseq_chronoseq_beads.html