title: Short Reads for The Genomic Landscape of Head and Neck Cancer-associated Streptococci
design: Following taxonomic identification using ONT LRS, isolates belonging to the Streptococcus genus were selected for SRS-WGS through the Australian Genome Research Facility (AGRF). Genomic DNA was enzymatically fragmented, and sequencing libraries were prepared using the Nextera XT DNA Library Prep Kit with unique dual indices added via low-cycle PCR. Libraries were purified, quantified, and manually normalised before sequencing. Paired-end sequencing (2  150 bp) was performed on the Illumina NovaSeq 6000 platform using S4 flow cell chemistry. Image analysis and base calling were performed in real time using NovaSeq Control Software (NCS v1.2.0.28691) and Real-Time Analysis (RTA v4.6.7). Raw base call (BCL) files were converted to FASTQ format using the Illumina DRAGEN BCL Convert pipeline (v4.0.3). All sequencing data met AGRF quality control standards.
library_name: Illumina_8633AN4