title: GSM8793784: MDA-MB-231_Control_1st_Rep; Homo sapiens; RNA-Seq
library_name: GSM8793784
library_construction_protocol: RNA was extracted from cell pellets containing (at least) 0.5 × 106 cells using the RNeasy Plus kit (#74134, Qiagen) following the manufacturer's protocol RNA sequencing (RNA-seq) libraries were prepared using the Stranded mRNA Prep, Ligation Kit (Illumina) according to the manufacturer's instructions. Briefly, mRNA was purified from 1 µg total RNA using oligo(dT) beads, poly(A)+ RNA was fragmented to 150 bp and converted into cDNA, and cDNA fragments were end-repaired, adenylated on the 3' end, adapter-ligated, and amplified with 12 cycles of PCR. The final libraries were validated using a Qubit 2.0 Fluorometer (Life Technologies) and a Bioanalyzer 2100 system. All barcoded libraries were pooled and sequenced 2x75bp paired-end on an Illumina NextSeq550 platform to obtain a minimum of 10 Mio reads per sample.