title: GSM8513026: mid_organoids8644113; Homo sapiens; RNA-Seq
library_name: GSM8513026
library_construction_protocol: For spatial transcriptomics: Fresh frozen tissue were OCT embedded, and 10µm frozen sections were cut with cryostat. For single-cell transcriptomics: samples were dissociated, treated with papain, equilibrated with 02:C02, minced, PBS wahed, dissociated again, triturated and finally pelleted For spatial transcriptomics: Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's construction. For single-cell transcriptomics: single cell suspensions were prepared and processed using the Chromium Single Cell 3' Reagent Kits v3