title: GSM9467235: E-2; Rattus norvegicus; miRNA-Seq
library_name: GSM9467235
library_construction_protocol: Total RNA was extracted from tissue samples using Trizol reagent (Invitrogen, USA) following the manufacturer's instructions. RNA purity and concentration were evaluated using a NanoDrop 2000 spectrophotometer (Thermo Scientific). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies). Small RNA libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer's protocol. Briefly, 1 µg of total RNA was used as input. 3' and 5' SR adaptors were ligated to the RNA, followed by reverse transcription and PCR amplification. The PCR products were size-selected using 6% polyacrylamide gel electrophoresis (PAGE) to isolate fragments of ~140-160 bp.