title: GSM9166638: Mesenteric arteries, normotensive Sphk1 flox/flox  2; Mus musculus; RNA-Seq
library_name: GSM9166638
library_construction_protocol: Total RNA from mesenteric arteries and aortas of WTf/f control (WTf/f Sham, n=4), AngII-infused WTf/f (WTf/f AngII, n=4), and AngII-infused SMC KO-Sphk1 (SMC KO-Sphk1 AngII, n=5) mice was isolated using PureLink™ RNA Micro Kit (Thermo Fisher, USA) and treated with DNase. The mRNA libraries were prepared using NEBNextUltra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, USA), and samples were sequenced on the Illumina NovaSeq 6000 System using a 2x150bp paired-end configuration.