title: GSM8633660: D14_rep2; Mus musculus; RNA-Seq
library_name: GSM8633660
library_construction_protocol: Explanted muscle samples were stabilized in RNAlater (Invitrogen) at -80°C prior to RNA extraction. Tissues were mechanically homogenized in Trizol, followed routine Trizol-based RNA isolation.  In brief, the lysed tissues were treated with chloroform, allowing for a biphasic separation of the RNA content, followed by RNA precipitation in isopropyl alcohol. The purified RNA was then resuspended in RNAase-free water. Bulk RNA sequencing was performed by Novogene Corporation, in which messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads.  cDNA was synthesized followed by non-directional library preparation for sequencing on a Novaseq 6000 instrument.