title: bacterial 16S rRNA gene V1-V9 amplicon-based microbiome profile of dental plaque-dervied in vitro culture
design: DNA was isolated from dental plaque-dervied in vitro cultures using the Fast DNA Spin Kit for Soil (MP Biomedicals Germany GmbH, Eschwege, Germany). Bacterial full-length 16S rDNA genes were amplified using the bacteria-specific primer pair 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT). Preparation for sequencing, including ligation of barcoded SMRTbell Adapters, followed PacBio protocols
library_name: ALS025__m54055_220218_224915