title: GSM9059431: Hs578T_CT5; Homo sapiens; RNA-Seq
library_name: GSM9059431
library_construction_protocol: Total RNA was extracted using the NZY miRNA Isolation & RNA Clean-up Kit (MB13402, NZYtech) following the manufacturer's protocol. The purity and concentration of the RNA from each sample were assessed using an Agilent TapeStation with an RNA ScreenTape Analysis Kit. mRNA enrichment was subsequently performed using polyA-tail-connected magnetic beads and oligos. This was followed by double-stranded DNA synthesis and polymerase chain reaction (PCR) amplification using specific primers. The PCR products were subjected to thermal denaturation to produce single-stranded DNA, which was cyclized into a circular DNA library using bridge primers. Sequencing was conducted using the DNBSEQ platform (BGI Genomics Co.).