title: RNA-seq of Homo sapiens: blood donors
design: The viral RNA was extracted from the serum of blood donors using the MagMax Viral Pathogen kit. The ds-cDNA was synthesized using the SuperScript VILO MasterMix combined with the NEBNext Second Strand Synthesis module. The ds-cDNA was purified using the PureLink PCR Purification kit, quantified with the Qubit Assay DNA HS Kit, and used for library construction with the TruSeq Nano DNA Kit. Sequencing was performed with the NextSeq 500/550 High Output Kit v2.5.
library_name: DD4