title: ITS-actB-29d replicate 3
design: Microbial community DNA was extracted from soil samples using the Quick-DNA Fecal/Soil Microbe Miniprep Kit  according to manufactureres' instructions (Zymo Research, Freiburg, Germany). 16s Amplicons of BrdU-marked "active" DNA samples (Immunocapture technique) and total DNA samples were generated with primer set fITS7 (5'-GTGARTCATCGAATCTTTG-3') (Ihrmark et al., 2012) and ITS4 (5'-TCCTCCGCTTATTGATATGC-3') (White et al., 1994).
library_name: ITS-Cob58