title: HT1080 N74D Capsid Mutant HIV Infected - Technical Replicate 3
design: HT1080-CPSF6-mNGreen cells were infected with VSV-G pseudotyped wild type or N74D capsid mutant HIV-1-NL4-3 at a multiplicity of infection (MOI) of 10 by spinoculation or mock-infected. RNA was extracted from each treatment group at 6 hours post-infection, and RNA samples were used for next-generation sequencing. Libraries were prepared using a NEBNext Ultra II kit with poly(A) selection, and samples were sequenced using a NovaSeq X Plus with 150 bp paired-end reads.
library_name: HT1080_N74D_Rep_3