title: GSM8759902: WT_GCMP1; Mus musculus; ATAC-seq
library_name: GSM8759902
library_construction_protocol: Nuclei were collected and resuspended into 50 μl tagmentation mix, which contains 10 μl 5×TTBL (Vazyme), 5 μl TTE Mix V50 and 35 μl ddH2O. Samples were incubated at 37 °C for 30 min. After tagmentation, fragmented DNA was isolated by using VAHTS DNA Clean Beads. ATAC libraries were amplified for 12 PCR cycles using TruePrepTM Index Kit V2 for Illumina (Vazyme #TD202). 0.55×VAHTSTM DNA Clean Beads were used to exclude the High–molecular weight DNA, followed by positive selection with 1×beads. High-quality DNA libraries were sequenced on the Illumina HiSeq2000.