title: RNA-seq of Equus caballus: Equine Endometrial Organoids and Tissue
design: Endometrial tissue (END) was processed for organoid culture, histology, IHC, and bulk- and single-cell RNA sequencing (RNAseq and scRNAseq, respectively). Organoids derived from seeding the single-cell suspension were classified as passage-0 (P0). After two subsequent passages, they were referred to as P2 organoids. To ensure sufficient organoid numbers for downstream analyses, including RNAseq, histology, IHC, and viability assessment, these organoids were allowed several days to regenerate and proliferate post-passage. Organoid harvesting followed the standard protocol used for passaging. Remaining organoids were cryopreserved in a solution containing 5% DMSO, 40% Fetal Bovine Serum (FBS), and 55% Advanced DMEM/F12. Cryopreserved samples were kept at -80C for 24 hours using Mr. Frosty freezing container before being transferred to liquid nitrogen for long-term storage.
library_name: Sample_9I_Diestrus_Tissue