title: Bos taurus Hanwoo PBMC Treponema spp. antigen stimulated sample from Animal3
design: Peripheral blood mononuclear cells (PBMCs) were isolated from clinically healthy Hanwoo cattle. This library was prepared from Animal3 under the Treponema spp. antigen-stimulated condition. For each animal, PBMCs were divided into paired unstimulated and stimulated aliquots. Stimulated samples were incubated for 12 h with a combination of whole cell sonicates from three bovine digital dermatitis-associated Treponema species. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Gold Kit with rRNA depletion and sequenced as 101 bp paired-end reads on an Illumina NovaSeq 6000 platform.
library_name: LIB_T3