title: GSM8487913: Intratumoral macrophage from mouse #746 with Cxcr4 KO in macrophage; Mus musculus; RNA-Seq
library_name: GSM8487913
library_construction_protocol: Primary tumor samples were snap frozen and homogenized. Total RNA were extracted using RNAeasy mini-prep kit from Qiagen The integrity of total RNA samples were assessed on Bioanalyzer 2100 using RNA 6000 Pico chip (Agilent Technologies, CA).  Messenger RNA was enriched from these samples using QIAseq FastSelect - rRNA HMR Kit (Qiagen, CA), fragmented and converted to cDNA and Illumina sequencing library using the PrepX RNA-seq library preparation protocol on the Apollo 324TM NGS Library Prep System (Takara Bio, CA).