title: RNA-Seq of sludge: SA
design: RNA extraction was performed using Soil RNA Extraction Kit (Majorbio, Shanghai, China). After RNA extraction, RNA concentration and purity were examined, RNA integrity was examined by 1% agarose gel electrophoresis, and RQN values were examined by Agilent 5300. The rRNA in the RNA samples was removed using the RiboCop rRNA Depletion Kit for Mixed Bacterial Samples (lexogen, USA) kit to obtain the target mRNA. rRNA was prepared using Illumina Stranded mRNA Prep, Ligation (Illumina, USA) for library construction and Illumina NovaSeq 6000 (Illumina, USA) sequencing platform for macro-transcript sequencing.
library_name: L1EIL1706268-SA