title: RNA-seq of Brucella abortus delta vjbR with DMF vehicle control
design: Bacterial cultures were grown to expontential phased, and stressed for ~30 minutes in the indicated chemical. The culture was then killed and stabalized with an equal volume of 1:1 ethanol::acetone. RNA was extracted via phenol:chloroform and purified with ethanol pricipitation. Ribosomal RNA was depleted, followed by RNA fragmentation and random priming. First and second strand cDNA was synthesized followed by end repair, 5' phosphorolaytion and dA-tailing. Adaptors were ligated on, followed by PCR enrichment and sequencing.
library_name: delta vjbR DMF 3