title: DNBSEQ-G400 paired end sequencing of SAMD00884446
library_name: TE_Sample2_CALHM3
library_construction_protocol: "To perform target enrichment of CALHM3 cDNA, we developed a custom targeted strategy employing 3-4 rounds of linear amplification and PCR in a nested manner. The amplified cDNA products were fragmented using Chromium GEM-X Single Cell 3' Reagent Kits v4 (10x Genomics) and were selected with SPRIselect reagent for subsequent library construction."