title: Whole-Genome Analysis of Escherichia coli Isolated from Milk and Meat Samples in the Greater Accra Region of Ghana
design: Library preparation involves essential materials such as nucleic acids (genomic DNA or cDNA), adapters for sequencing compatibility, enzymes (DNA polymerase, restriction enzymes, T4 DNA ligase) for fragmentation and ligation, buffers and purification kits for optimal reaction conditions, and size selection tools (agarose gel electrophoresis, magnetic beads) for isolating appropriately sized fragments. Key methods include fragmentation of DNA, end repair and A-tailing for adapter preparation, ligation of adapters to DNA fragments, and size selection to purify and select the desired fragment sizes.
library_name: S8_L001