title: Clinical and molecular characterization of vancomycin-resistant enterococci from immunocompromised host and impact on human cell viability in an in vitro colon model
design: DNA extraction was carried out following the manufacturers practice provided by QIAGEN QIAamp DNA Mini Kit (Ref. 51304, QIAGEN, 40724 Hilden, Germany). DNA was quantified using both the Eppendorf BioPhotometer D30 and the fluorimeter Qubit dsDNA BR Assay Kit (Ref. 32850, Invitrogen, 92008 Carlsbad, CA, USA).A concentration of 100 ng of each sample have been used for sequencing by NGS. This was carried out on a Illumina MiSeq platform according to the manufacturers instructions provided in the Illumina DNA Prep  (M) Tagmentation (24 Samples) for Illumina (Ref. 20018707, Illumina, Inc., 92122, San Diego, CA, USA). Indexes were provided with Nextera DNA CD Indexes Illumina (24 Indexes, 24 Samples) (Ref. 20019105, Illumina, Inc., 92122, San Diego, CA, USA). Denature and dilute libraries were performed following the "Denature and Dilute Libraries Guide" protocol provided by Illumina (Document # 1000000025416 v09), choosing as Loading Concentration 8,5 pM. Finally, sequencing was performed using the MiSeq Reagent Kits v3 (Ref. 15043895, Illumina, Inc., 92122, San Diego, CA, USA).
library_name: Ef29