title: GSM8792070: WT Hela cells, ATAC-seq, biol rep 1; Homo sapiens; ATAC-seq
library_name: GSM8792070
library_construction_protocol: The nuclei were extracted from G1/S-transition synchronized cells using lysis buffer and centrifuged at 500×g for 5 minutes at 4°C. ATAC-seq was performed using the Vazyme Hyperactive ATAC-Seq Library Prep Kit for Illumina (#TD711) according to the manufacturer's instructions.The nuclei were extracted from G1/S-transition synchronized cells using lysis buffer and centrifuged at 500×g for 5 minutes at 4°C. Nuclei pellets were resuspended in the Tn5 transposase reaction mix and incubated at 37°C for 30 minutes. Following transposition, equimolar adapters were ligated, and the resulting libraries were amplified by PCR. Libraries were purified using AMPure beads and quantified using a Qubit fluorometer. Sequencing was conducted on an Illumina NovaSeq 6000 platform with 150 bp paired-end reads.