title: GSM8549020: Mut_Th2_1; Mus musculus; ATAC-seq
library_name: GSM8549020
library_construction_protocol: Sorted naïve CD4 T cells were activated and expanded using anti-CD3 (1ug/mL) and anti-CD28 (3ug/mL) in complete IMDM media in presence of antigen presenting cells (mitomycin C treated T-cell depleted splenocytes) Ten thousand cells were pelleted and washed with 50 μL 1 × PBS. After lysis, nuclei were pelleted by centrifuging at 500 × g for 10 min, and re-suspended in 50 μL transposase mixture (25 μl of 2x TD buffer, 2.5 μl of TDE1, 0.5 μl of 1% digitonin, 22 μl of nuclease-free water) (#20034197, Illumina; #G9441, Promega) for incubattion at 37°C with shaking at 300 rpm for 30 min. The tagmentated DNAs were then purified using a QIAGEN MinElute kit and amplified with 10 or 11 cycles of PCR based on the amplification curve