title: Bulk RNAseq of Rhesus PFC Exposed to THC Prenatally
design: RNA extracted from frozen lung samples was sent to Novogene for RNA QC, library preparation, and sequencing.  Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were quantified with Qubit and real-time PCR and assessed for size distribution with a bioanalyzer. Quantified libraries were pooled and sequenced on an Illumina NovaSeq 6000 with 30M (150bp paired-end) reads per sample.
library_name: C_RNA_6