title: GSM8760243: Human iMPC-derived chondrocyte #1; Homo sapiens; RNA-Seq
library_name: GSM8760243
library_construction_protocol: For bulk RNA‐seq experiments, cell pellets made by iMPCs or MSCs were induced in the chondrogenic medium (containing TGFβ3 or TGFβ+BMP4) for 56 days, and then lysed in QIAzol from the RNeasy Plus Mini Kit (Qiagen, Germantown, MD, USA) for RNA extraction. Extracted RNA were quantitated Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific) followed by the RNA quality check using Fragment Analyzer (Agilent Technologies, Santa Clara, CA). For each sample, RNA libraries were prepared from 500ng RNA using the KAPA mRNA HyperPrep Kit (Roche, Indianapolis, IN) according to manufacturer's protocol, followed by quality check using Fragment Analyzer (Agilent Technologies) and fluorescent quantification on the Infinite F Nano + (Tecan, Männedorf, Switzerland). The libraries were normalized and pooled, and then sequenced using NovaSeq6000 platform (Illumina, San Diego, CA,) to an average of 50M 100PE reads.