title: GSM9168092: Multiome, heart, LPS and vehicle,ATAC; Mus musculus; ATAC-seq
library_name: GSM9168092
library_construction_protocol: Nuclei were extracted from hearts using the Chromium Nuclei Isolation kit (10x Genomics). For sample preparation on the 10x Genomics platform, the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns PN-1000283, Chromium Next GEM Chip J Single Cell Kit, 48 rxns PN-1000234, Single Index Kit N Set A, 96 rxns PN-1000212 (ATAC), Dual Index Kit TT Set A, 96 rxns PN-1000215 (3v3.1 GEX), were used. Multiome 3v3.1 GEX and ATAC libraries were prepared as recommended by 10x Genomics (ChromiumNextGEM Multiome_ATAC_GEX_User_Guide_RevD). The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer's protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument.