title: GSM9261343: Transcriptome pool samples from brain of 3 months‑old mice (wild type and CSTB‑deficient); Mus musculus; RNA-Seq
library_name: GSM9261343
library_construction_protocol: Total RNA was extracted from frozen brain tissue using the RNeasy Plus Mini Kit (Qiagen), following the manufacturer's protocol. Tissue was homogenized in RLT Plus buffer with 2-mercaptoethanol using a SpeedMill PLUS bead homogenizer. RNA integrity was assessed using Agilent Tapestation. Libraries were prepared using a Drop-seq-based bulk RNA-seq protocol (as in Macosko et al., 2015 and Freitag et al., 2020). Sequencing-ready libraries were generated with sample-specific barcodes and unique molecular identifiers (UMIs) to enable multiplexing.