title: GSM8547869: Lung, samples 9, 13-16; Mus musculus; RNA-Seq
library_name: GSM8547869
library_construction_protocol: Mice lungs were digested Mice lungs were harvested, minced with a razor blade, digested in Collagenase IV (1.6mg/ml) + DNAse I (10ug/ml) in DMEM buffer supplemented with Hepes (10mM) for 30 min. Lungs were triturated with a 1mL pipette and cell suspensions were filtered (40um). 2 different mice were pooled for each sample. RBC were lysed (Gibco, #A10492-01) before staining with viability dye (Biolegend 423105) and anti-CD45 (103107). 500000 live immune cells were sorted on a FACS ARIA III (BD) and harvested in collection buffer (PBS+ 0.04% BSA + 50% FBS). Cells were then fixed, before single cell library preparation using Chromium 10x fixed RNA kits and instructions (#PN-1000414 and #PN-1000497).