title: mtscATAC-seq of MSK-143 PBMC-derived monocytes
design: Immune cells were first isolated from dissociated glioma samples via CD45+ MACS followed by FACS purification for mGAM and non-mGAM populations. Peripheral blood from the same patient was collected and PBMCs isolated using gradient separation (Ficoll-Paque PLUS, Cytiva, USA) followed by CD14 MACS sorting to obtain monocytes. Purified viable cells were used as input to the mtscATAC-seq protocol, and libraries were prepared using the Chromium Next GEM single-cell ATAC reagent kit v2.0 following the manufacturers instructions with the modifications described in the mtscATAC-seq protocol 60,122. Library size and quality were analyzed before fragmentation and after final purification. Libraries were sequenced on an Illumina NovaSeq X (Illumina, USA).
library_name: MSK-143_mono