title: GSM9057764: Tumor-bearing Gprc5a-/- lung tissue 8 [ST-D1]; Mus musculus; OTHER
library_name: GSM9057764
library_construction_protocol: Visium ST experiment n = 13 samples: For Gprc5a−/− mouse lung tissues analyzed by ST, we studied mice at EOE and at 7 months post exposure to either saline or NNK (2 mice per treatment and timepoint with each mouse lung split across two 6.5 x 6.5 mm regions of interest/capture areas; n = 8 mice; n = 16 libraries total). ST data was generated from 2 regions per mouse (i.e. 4 regions per treatment and timepoint) using 10x Genomics' Visium platform (6.5 × 6.5 mm ROI and capture areas) and following manufacturer's recommendations outlined in Visium Spatial Gene Expression Reagent Kits for FFPE User Guide (10x Genomics, CF000407). Visium ST experiment n = 6 samples: For ST profiling of lungs of NNK-exposed Gprc5a−/− mice which received anti- IL-1𝛽 or control IgG and analyzed at 3 and at 7 months post end of NNK, we employed the Visium CytAssist platform from 10x Genomics. One 11 × 11 mm region of interest was selected for each mouse lung and ST was performed on the selected archived and de-cover-slipped H&E slides by carefully positioning the 10x Genomics CytAssist gaskets to select and process these regions of interest. ST profiling was done following manufacturer recommendations outlined in Visium CytAssist Spatial Gene Expression Reagent Kits User Guide (10x Genomics, CG000495). cDNA