title: Bacterial 16S rRNA amplicon data sampled and sequenced from the National Park of the Tablas de Daimiel (Spain)
design: DNA extraction was performed using a DNeasy PowerSoil kit (Qiagen) following manufacturer instructions. To amplify the bacterial V3-V4 region, we used the primer pair Bact341F (5-CCTACGGGNGGCWGCAG-3) and Bact785R (5-GACTACHVGGGTATCTAATCC-3). PCR conditions were as follow: initial denaturation at 95C for 3 minutes, followed by 25 cycles of annealing (95C for 30 seconds, 55C for 30 seconds, and 72C for 30 seconds), and a final extension at 72C for 5 minutes.
library_name: Bact_CB-F_341F-785R