title: GSM8861741: BlaER1, WT, TAK, REP2; Homo sapiens; RNA-Seq
library_name: GSM8861741
library_construction_protocol: Total RNA was extracted using 1mL Trizol reagent and 100uL 1-Bromo-3-chloropropane (BCP). The upper phase was collected, and RNA was precipitated using 2-propanol, washed with 80% ethanol, resuspended in water and quantified. cDNA was generated using 1ug RNA and a High-Capacity cDNA Reverse Transcription Kit (Applied  Biosystems) and diluted in 200uL water. Total RNA-seq libraries were generated using Library prep stranded total RNA Ribozero Plus Ligation (Illumina) following manufacturer's instructions