title: Bacillus thuringiensis
design: WGS of bacterial isolate. Genomic DNA extraction was performed according to the GeneJet Genomic DNA purification kits protocol. The concentration and quality of extracted DNA was determined using Nanodrop Lite spectrophotometer (Thermo Scientific, Wilmington, DE, USA), Qubit dsDNA BR assay (Thermo Fisher Scientific, Wilmington, DE, USA) and agarose gel electrophoresis. Libraries were prepared with the Oxford Nanopore Rapid Barcoding Kit 24 v14 (SQK-RBK114.24) following the manufacturers protocol (PCR-free, transposase-mediated tagmentation with inline barcodes). Barcoded libraries were pooled equimolarly without size selection. The pooled library was loaded onto a FLO-MIN114 (R10.4.1) flow cell and sequenced on a MinION Mk1C under MinKNOW control. Basecalling and demultiplexing were performed with ONT Dorado (Q20+ sup model for R10.4.1) with adapter/barcode trimming enabled; pass reads were retained for downstream analysis.
library_name: CRE1-NanoP