title: GSM8547985: fetal liver, E14.5, BulkRNA-seq, CD127posRunx3negRORgtneg_gtposRunx3neg_3; Mus musculus; RNA-Seq
library_name: GSM8547985
library_construction_protocol: Runx3-negative CLPs fractions and RORγt-mid fractions were each cultured on TSt4/DLL4 for 10-12 days. After collection by pipetting, the RORγt-positive cells with or without Runx3 expression were re-sorted respectively. Bulk RNA-seq was performed on 50-200 sorted cells using the SMART-Seq HT PLUS Kit following the manufacturer's instructions (Takara Bio).