title: GSM8603802: Lactate; Mus musculus; OTHER
library_name: GSM8603802
library_construction_protocol: NaCl or lactate (25 mM) treated neuro2a cells were collected and bounded to Concanavalin A–coated beads. Subsequently, cells were resuspended in antibody buffer and incubated with primary antibodies against H4K12la and secondary antibodies in order. The samples were incubated with pA-Tn5 transposase. After transposon activation and tagmentation, DNA was isolated, amplified, and purified to construct library. The library for sequencing was constructed and VAHTS DNA Clean Beads (N411, Vazyme Biotech) were used for purification steps. The library was quantified with VAHTS Library Quantification Kit for Illumina (Vazyme Biotech) and sequenced on an Illumina Novaseq 150PE system.