title: GSM8760258: JHUBK5; Homo sapiens; RNA-Seq
library_name: GSM8760258
library_construction_protocol: Libraries were prepared using the NEBNext UltraExpress RNA Library Prep Kit (New England Biolabs, Ipswich, MA), following the manufacturer's protocol. mRNA was enriched using poly(A) selection. Library quality and size distribution were assessed using a Tapestation (Agilent) and quantified by Qubit (Thermo Fisher Scientific). Final libraries were pooled equimolarly and sequenced on an Illumina NovaSeq X Plus platform using a 25B lane configuration with paired-end 150 bp (PE150) reads with a minimum of 20M Reads per sample.