title: GSM8793119: LH tissue from subject KM198; Mesocricetus auratus; RNA-Seq
library_name: GSM8793119
library_construction_protocol: Samples were homogenized in 100 μl lysis buffer (Thermo Fisher Scientific, Waltham, MA; MagMax Total RNA isolation kit, Cat. No. A27828) with 0.7% beta-mercaptoethanol by vortexing at 3000 rpm speed for 15–20 s. Lysates were incubated at room temperature for 5 min and stored at − 80°C until RNA extraction. Once dissection of all individuals was completed, we proceed to RNA extraction using the KingFisher Flex (Thermo Fisher Scientific, Cat. No. 5400630l) with an additional DNase step to remove DNA contamination added according to the manufacturer's protocol. Samples from each brain region of all animals were extracted in the same batch. RNA quality was determined using RNA 6000 Nano Assay with BioAnalyzer (Agilent Technologies, Santa Clara, CA) and RNA concentration was determined with Quant-it RNA High Sensitivity assay kit (Thermo Fisher Scientific, Cat. No. Q33140). RNA samples were normalized to 100 ng/μl and stored at − 80°C before sequencing. All samples were processed in the same batch throughout the process. Libraries were constructed with a protocol modified from (Meyer et al., 2011) and (Lohman et al., 2016). Reads were sequenced on the NovaSeq 6000 SR100 with minimum reads of 4 million and the target reads per sample of 5 million.