title: GSM8993670: RPE-1 hTERT FUCCI H3K27me3 rep 4 no TAPS ctrl; Homo sapiens; OTHER
library_name: GSM8993670
library_construction_protocol: TrypLE digestion for RPE-1 hTERT FUCCI cells Custom library protocol. Briefly, cells were incubated with the antibodies listed above and a pA-MNase fusion protein. Next, individual cells were sorted into 384-well plate. Subsequently, MNase digestion was initiated through the addition of calcium. Next, MNase digestion was stopped and DNA released by Proteinase K digestion. DNA fragments were end-repaired by Klenow Large Fragment and PNK. Repaired ends were ligated to custom barcoded adapters containing a UMI, Illumina handle and T7 promoter. After IVT and PCR, sequencing libraries were assessed with Qubit and Bioanalyzer and normalized libraries loaded onto Illumina sequencer according to the manufacturers instructions.