title: a plant
design: High-quality total DNA was extracted from fresh leaves, and its purity and quantity were evaluated using the NanoDrop 1000 and Qubit from ThermoFisher Scientific. A 15-kb library was constructed using a SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences, CA, USA). The construction included DNA shearing, AMPure PB bead purification, ssDNA overhang removal, damage repair, end repair, hairpin adapter ligation, and library bead purification. Following quality control, a SMRTbell library was obtained. The library was sequenced on the PacBio Revio platform (Pacific Biosciences, CA, USA). Raw data were processed using the CCS algorithm (version 6.0.0, parameters: minPasses 3  minPredictedAccuracy 0.99 maxLength 21,000) to generate highly accurate HiFi reads.
library_name: 10