title: GSM8685903: LNCaP_sgT33-5_MC_C2_DMSO_RNA-seq_rep2; Homo sapiens; RNA-Seq
library_name: GSM8685903
library_construction_protocol: RNA was extracted using the RNeasy mini kit (Qiagen) following manufacture instructions. Quality of the RNA extraction was assessed using the 2100 Bioanalzer (Agilent, RNA 6000 Nano Kit) and selecting samples with a RNA Integrity Number (RIN) above 9. PolyA+ stranded RNA library was prepared using the Illumina Stranded mRNA Prep kit and quality was assessed by using the 2100 Bioanalyzer (Agilent, DNA 7500 kit). RNA-seq libraries have been pooled equimolarly and sequenced using NovaSeq6000 (Illumina) sequencer with a 51bp Paired-End reads setup.