title: Scraping-derived Organoid DNA from Subject hT316
design: The whole exome library preparation was performed using the IDT xGEN DNA library preparation and hybridization following the manufacturer's instructions. Briefly, 100 ng of purified DNA isolated from PDOs was sheared to 400 ng using a Covaris S2 sonicator, followed by a series of AMPure XP bead purifications, after End Repair and ligation of unique barcodes via 11 PCR cycles.  Libraries were Qubit quantified, pooled equi-mass and hybridized overnight with blocking oligos with IDT xGEN Exome Hyb panel V2 capture probes.  Targeted sequences were bound with streptavidin beads, underwent additional PCR amplification and purification, and final QC performed using Qubit quantification and Tape Station qualification. Sequencing was performed using the Singular platform, the library pools were converted using Singular-specific primers and 6 PCR cycles. A final QC was completed before sequencing. The WES pool was sequenced in three F3 flowcells Singular G4 PE150.
library_name: hT316-S-PDO-DNA