title: GSM8760331: BaF3 cells, NRAS G12D, ETV6 1; Mus musculus; RNA-Seq
library_name: GSM8760331
library_construction_protocol: RNA was harvested using Zymogen Direct-zol RNA Miniprep Kit. Library's were constructed by Novogene Co. using the following methods: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.