title: GSM8895342: Monocytes, 125D+LPS, non-primed, ATAC replicate 2; Homo sapiens; ATAC-seq
library_name: GSM8895342
library_construction_protocol: ATAC-seq: nuclei were isolated from 1.25 × 10⁶ cells using Buffer A (15 mM Tris-HCl, pH 8.0; 15 mM NaCl; 60 mM KCl; 1 mM EDTA; 0.5 mM EGTA; 0.5 mM spermidine; 1% protease inhibitor) supplemented with 0.04% IGEPAL. A total of 100,000 nuclei were subjected to transposition using a Tn5 transposase reaction mix (Illumina), containing Tn5 transposase, 0.01% digitonin, and 0.1% Tween-20. The reaction was incubated for 30 minutes at 37 °C in a Thermomixer. Following purification with the DNA Clean & Concentrator-5 Kit (Zymo Research), the ATAC-seq libraries were amplified via PCR, purified, and size-selected using SPRISelect purification beads (Beckman Coulter). Library quality was assessed with a Bioanalyzer (Agilent High-Sensitivity DNA Kit), and the quantified, indexed libraries were pooled for sequencing. ATAC-seq  libraries were sequenced on an Illumina NextSeq500 system (Illumina) at 75 bp read length using standard protocols at the Gene Core facility of the EMBL (Heidelberg, Germany).