title: GSM8547801: Hepatoblast, Myh9 siRNA-transfected, replicate 1; Mus musculus; RNA-Seq
library_name: GSM8547801
library_construction_protocol: RNA was isolated using the RNeasy Mini Kit (Qiagen; 74104) including the DNase I (Qiagen; 79254) treatment. Cells were lysed with RNeasy lysis buffer supplemented with DTT. mRNA was isolated from the total RNA by poly-dT enrichment using the NEBNext Poly(A) mRNA Magnetic Isolation Module according to the manufacturer's instructions, fragmented to 350 nt, and libraries were prepared using the NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina. For Illumina flowcell production, samples were equimolarly pooled and distributed on all lanes for 100bp paired-end sequencing on Illumina NovaSeq 6000.