title: 10x of female Trichiurus japonicus, liver
design: Fish liver tissue is dissociated into a single-cell suspension, filtered, and viability-checked. Cells are loaded onto the 10x Genomics Chromium system for GEM generation, barcoding, and reverse transcription. cDNA is amplified, fragmented, ligated, and indexed to construct libraries, followed by quality control and sequencing on an Illumina platform.
library_name: 10x