title: H3K4me3 ChIP-seq of Emu: adult female left ovary
design: Native ChIP-seq of H3K4me3 histone marks was performed following the protocol described in Saleh et al. 2008. ChIPed and input DNA were purified using phenol/chloroform/isoamyl alcohol (25:24:1) extraction and ethanol precipitation, followed by dissolution in H2O. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, NEB, #E7645) and sequenced with paired-end 150 bp reads on the Nova HiSeq2500 platform (Illumina, China, Novagene).
library_name: Emu_F_L_O_H3K4me3