title: WGS of P.putida phage
design: For Nanopore sequencing, the genomic DNA was end-polished and A-tailed using NEBNext ultra II end repair kit (NewEnglandBiolabs, MA, USA). The end-prepared samples were barcoded using Blunt TAligase master mix (M0367L). The library was prepared using a ligating sequencing kit (SQL-NBD 114.24) from Oxford Nanopore Technologies (ONT) double-stranded DNA fragments using NEB Quick T4 DNA Ligase (New England Biolabs, MA, USA). Purification of the samples were done with AMPure XP beads. The prepared library was quantified using Qubit and sequenced on Nanopore PromethION system, (PromethION P24 and Data Acquisition Unit, ONT, Oxford, UK) using PromethION flow cell (FLO-PRO114M).
library_name: Nanopore