title: GSM8862142: THP-1 cells,infected with H37Rv,Day1, treated with 1,25(OH)2D3-2; Homo sapiens; RNA-Seq
library_name: GSM8862142
library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit (Qiagen, GER,74106). Paired-end libraries were synthesized using the TruSeq™ RNA Sample Preparation Kit (Illumina, USA, RS-122-2001) following the TruSeq™ RNA Sample Preparation Guide. In brief, poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads. Subsequently, the mRNA was fragmented into small pieces using divalent cations at 94℃ for 8 min. The cleaved RNA fragments were then used to generate first-strand cDNA with reverse transcriptase and random primers. This was followed by the synthesis of second-strand cDNA using DNA Polymerase I and RNase H. The resulting cDNA fragments underwent an end repair process, the addition of a single 'A' base, and ligation of the adapters.