title: RNA-Seq of mus musculus: adult male liver
design: RNA sequencing (RNA-Seq) was performed to profile the hepatic transcriptome in response to pharmacological PPAR activation and its role in acesulfame-K-induced NAFLD. Liver tissues were collected from four experimental groups: wild-type (WT) and PPAR-null (KO) mice treated with either the PPAR agonist Wy-14,643 or a vehicle control, with three biological replicates per group (12 samples total). RNA was extracted, and libraries were constructed using a standard protocol involving mRNA enrichment via poly-A selection, fragmentation, cDNA synthesis, and adapter ligation. Sequencing was carried out on an Illumina platform (HiSeq series) to generate 150-bp paired-end reads.
library_name: WT_CON_3