title: GSM8828011: HSJD-DIPG-007 Day 5 6 Gy; Homo sapiens; RNA-Seq
library_name: GSM8828011
library_construction_protocol: Cells were stored in RLT with beta mercaptoethanol snap frozen at -80. Gentle thawing, and RNA extraction was carried out as per instructions, RNA was extracted from both non-irradiated (proliferative) and irradiated (senescent) human DMG cells using the Rneasy Micro kit (Qiagen). Total RNA integrity was validated using Agilent's 4200 Tapestation, with all samples having RIN values >9.4. UCL genomics core facility prepared cDNA libraries using 250ng of total RNA and the KAPA mRNA HyperPrep Kit. High-quality libraries were confirmed on the Agilent TapeStation 4200. Samples were pooled, normalized to 4nM using the Normalase assay, and sequenced on the NextSeq 500 instrument with a 75bp paired-end run.