title: GSM9058996: KO3; Mus musculus; RNA-Seq
library_name: GSM9058996
library_construction_protocol: Microglia were FACS-sorted from whole brains, into a collection tube and then total RNA was extracted using PicoPure RNA Isolation Kit (KIT0204, Life Technologies) according to manufacturer's protocol. Library preparation was carried out as described in the Illumina Nextera XT Sample Preparation Guide (Illumina, Inc., San Diego, CA, USA). Thereby 150 pg of input cDNA were tagmented (tagged and fragmented) by the Nextera XT transposome. The products were purified and amplified via a limited-cycle PCR program to generate multiplexed sequencing libraries. For the PCR step 1:5 dilutions of the unique dual indexing (i7 and I5) adapters were used. The libraries were quantified using the KAPA Library Quantification Kit - Illumina/ABI Prism User Guide (Roche Sequencing Solutions, Inc., Pleasanton, CA, USA).