title: AML WXS
design: Genomic DNA was isolated from cultured patient AML cells. Library preparation started with shearing input DNA into smaller fragments, followed with fragment end-repair, dA tailing, ligation of index adaptors, and amplification. The libraries were then hybridized, captured, and amplified with the QIAseq Human Exome probe set following the QIAseq Human Exome Handbook (Qiagen). Each resulting captured library was quantified and its quality accessed by Qubit and Agilent Bioanalyzer. Multiple libraries were pooled in equal molarity and sequenced on an Illumina NovaSeq 6000 sequencer with 150 bp paired-end reads. The GATK was used for SNP and insertion/deletion (indel) discovery according to GATK best practices.
library_name: 0807-08