title: GSM8686461: 1376_infected; Mus musculus; RNA-Seq
library_name: GSM8686461
library_construction_protocol: For RNA extraction, cells were collected and placed in TRIzol™ for SARS-CoV-2 inactivation. RNA was extracted from TRIzol™ and then column purified (QIAGEN, RNeasy Kit 74106). 2μg total RNA was used for the contstruction of the sequecncing libraries. The paired-end 150 sequencing reads generated by Illumina sequencing are mapped to the genome using the STAR algorithm with default settings. Alignment information for each read is stored in the BAM format. nfcore/rnaseq pipeline is applied to perform the alignment.