title: GSM8861769: Erythroid cells ffrom alpha thalassemia major donor, transduced with A2 UV renamed EV-a-UV; Homo sapiens; RNA-Seq
library_name: GSM8861769
library_construction_protocol: On day 16 of in vitro erythroid differentiation, 120,000 cells were collected and resuspended in 100uL of Phosphate Buffered Saline (PBS) (Corning) with 0.05% Bovine Serum Albumin (BSA) (Sigma Aldrich, Cat# A7979-12) for single cell RNA sequencing conducted by the UCLA Technology Center for Genomics and Bioinformatics Single-cell libraries were constructed using the Chromium Next GEM Single Cell 3' Kit v.3.1 (10X Genomics) targeting 10k cells, following manufacturer's protocol. The libraries were sequenced on the Illumina NovaSeq X+ paired-end platform (on a 10B flow cell with 150+10+24+150 cycles) and data de-multiplexing was performed using BCLConvert software v4.3.13 (Illumina).