title: Single Nucleus RNA-seq of soybean leaf
design: Nuclei isolation was performed from Si+ and Si- soybean leaf tissues as previously described (DAgostino et al., 2023). Briefly, the tissue was ground in a nuclei isolation buffer and incubated briefly on a slow shaker. After incubation the nuclei suspension is passed through 100 m, 70 m, 30 m, and 10 m cell strainers. The suspension was spun at 1000g for 5 minutes. The nuclei pellet was resuspended in wash buffer and spun again at 1000g for 2 minutes. The nuclei were stained with propidium iodide and counted with the Luna-FL (Logos Biosystems). The nuclei were then loaded onto the 10x Genomics chip as per 10x genomics recommendations
library_name: L1