title: ScRNA-Seq of mus musculus: adult male colon
design: ScRNA-seq libraries were prepared using the DNBelab C Series High-throughput Single-Cell RNA Library Preparation Set (MGI, 940-001818-00), following a workflow that included single-cell suspension processing, droplet encapsulation, emulsion breakage, mRNA capture, reverse transcription, cDNA amplification, and purification. The amplified cDNA was sheared into 300-500 bp fragments, followed by indexed library construction according to the manufacturers protocol, with quality assessment performed using the Qubit ssDNA Assay Kit (Thermo Fisher Scientific) and Agilent Bioanalyzer 2100.
library_name: A95