title: GSM8793349: Cultured CDSCs; Mus musculus; RNA-Seq
library_name: GSM8793349
library_construction_protocol: The primary and cultured CDSCs at passaged P2-P3 underwent single cell RNA-seq (scRNA-seq) (MobiDrop, China). Briefly, the cell viability was determined by staining with Trypan blue (Beyotime, C360I-2) under a microscope. 20000 viable cells were prepared for application to a Chromium Controller (10x Genomics, USA), where the chromium Single Cell 3' v3.1 kit (1000121, 10x Genomics, USA) was used to generate oligo (dT)-primed cDNA libraries. These libraries were quantified using the Agilent TapeStation High sensitivity D5000 ScreenTape and subsequently analyzed via Next Generation Sequencing (Nova-Seq 6000, Illumine, USA) with a data output of 200G.