title: GSM9466786: ESI017, PPS, 24hr, rep1; Homo sapiens; RNA-Seq
library_name: GSM9466786
library_construction_protocol: Cells were dissociated with Accutase, pelleted at 1000 rpm for 4 min, and lysed in Lysis Solution. Pellets were snap-frozen in liquid nitrogen and stored at −80 °C until processing. Total RNA was extracted using the RNAqueous-Micro Total RNA Isolation Kit (Invitrogen) according to the manufacturer's protocol and stored at −20 °C until library preparation. RNA libraries for RNA-seq were prepared using the Illumina poly(A)-selected stranded mRNA workflow (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, NEB) following the manufacturer's protocol. Sequencing was performed as paired-end 2 × 75 bp reads on an Illumina NextSeq 2000 platform by Novogene Corporation Inc.