title: scATAC-seq of first and second trimester human reproductive tract
design: scATAC-seq of first and second trimester human reproductive tract
library_name: HCA_F_GON11173192_and_HCA_F_GON11212447_p
library_construction_protocol: Single-nuclei suspensions were isolated from dissociated cells when performing scATAC-seq, following the manufacturers' instructions, and from frozen tissue sections when performing multiomic snRNA-seq/scATAC-seq. For the latter, thick (300 µm) sections were cryosectioned, and kept in a tube on dry ice until subsequent processing. Nuclei were released via Dounce homogenisation as described in detail in the protocols.io.  For the scATAC-seq and multimodal snRNA-seq/scATAC-seq experiments, cells were loaded according to the manufacturer's protocol for the Chromium Single Cell ATAC v2  and Chromium Next GEM Single Cell Multiome ATAC+Gene Expression (10X Genomics) to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer's protocol. 