title: GSM8574735: HDH-TnFa,rep1,scRNAseq; Rattus norvegicus; RNA-Seq
library_name: GSM8574735
library_construction_protocol: 1. Remove the cryovial from the liquid nitrogen container and place it directly into a 37 ℃ water bath. Shake it from time to time to melt it as quickly as possible. 2. Remove the cryovial from the 37 ° C water bath, open the lid, use a pipette to aspirate the cell suspension, and add it to the centrifuge tube. 3. Centrifuge, 300rcf, 3.5min: 4. Discard the supernatant, add 1ml PBS+0.01% BSA to resuspend the cells, centrifuge at 300rcf for 3.5 minutes. 5. Repeat step 4 twice 6. Count and use as needed. Sequencing libraries were prepared according to the manufacturer's instructions of 10K Genomics Library Amp Kit