title: PacBio HiFi sequencing data from female Sorex ornatus (CJC3344/MVZ:Mamm:240319)
design: The HiFi SMRTbell libraries were constructed using the SMRTbell Express Template Prep Kit v2.0 (Pacific Biosciences, Menlo Park, CA; Cat. #100-938-900) and SMRTbell prep kit 3.0 (Pacific Biosciences, Menlo Park, CA; Cat. #102-182-700) according to the manufacturer's instructions.  HMW gDNA was sheared to a target DNA size distribution between 15 kb  18 kb using Diagenodes Megaruptor 3 system (Diagenode, Belgium; cat. B06010003). The sheared gDNA was concentrated by AMPure PB beads (Pacific Biosciences, Menlo Park, CA; Cat. #100-265-900) for multiple enzymatic reactions such as removal of single-strand overhangs, DNA damage repair, end-repair/A-tailing, overhang adapter ligation, and nuclease treatment. The first two SMRTbell libraries were size selected using the BluePippin/PippinHT system (Sage Science, Beverly, MA; Cat #BLF7510/HPE7510) according to the v2.0 protocol to collect fragments greater than 7-9 kb. The third SMRTbell library was size selected using 3.1X of 35% v/v diluted AMPure PB beads to progressively remove SMRTbell templates <5kb according to the SMRTbell prep kit 3.0 protocol. The HiFi SMRTbell libraries were sequenced at UC Davis DNA Technologies Core (Davis, CA) using 5 SMRT cells (Pacific Biosciences, Menlo Park, CA; Cat #101-389-001), Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.
library_name: CCGP_gDNA0071_2