title: Microorganism
design: The microorganism was sequenced at the Genomic Sequencing Center of the University of Antioquia  Colombia. They used sequencing of the extracted genomic DNA from bacterial strains, employing Truseq Nano DNA (350) libraries and the Illumina NovaSeq platform, which generated paired-end reads of 150 bases. This analysis confirmed the species of the microorganism of interest, which was Enterobacter hormaechei subsp. xiangfangensis. Additionally, the bioinformatic analysis began with the fastq files, which were reviewed using FastQC to assess the quality of the reads. Then, assembly was performed using the SPADES and MEGAHIT programs. No significant differences were found between the two, as they were initially analyzed with the BUSCO program, which evaluated the completeness of the assembly by comparing it to conserved genes of specific lineages. The assembly was then further analyzed using the QUAST program to assess the quality of the assembly in terms of contig N50, total size, assembly errors, and other quality metrics, where no differences were observed. Based on this, it was decided to work with the SPADES assembly. The next step was annotating the assembly to assign functions and descriptions to the present genes, using the Prokka program for prokaryotic organisms.
library_name: B-S65-1