title: GSM8760226: cKO_sample1; Mus musculus; RNA-Seq
library_name: GSM8760226
library_construction_protocol: Skin tissues were harvested and placed inside aluminum cylinders (5 mm inner diameter) that had been cleaned with RNase Away (Molecular Bio Products) and filled with 60 μL of RNase-free ultrapure water. The cylinders were submerged in liquid nitrogen for 1 minute to freeze the tissues. The frozen tissues were stored at -80°C and transferred to a cryostat for sectioning prior to RNA extraction. The frozen sample was removed from the cylinder while keeping it on ice and mounted on a cryostat chuck using OCT compound. Approximately 20 frozen sections (20 μm each) were carefully cut and transferred to pre-chilled 2 mL tubes using cold tweezers. This process was repeated until the entire sample was sectioned. After sectioning, 1 mL of QIAzol was added to the tubes, and the contents were vortexed for 60 seconds. The samples were kept on ice until RNA purification. RNA was isolated and purified using the Quick-RNA Micro Prep Kit (Zymo Research), and RNA concentration and purity were evaluated using a Bioanalyzer (Agilent 2100). libraries were constructed by Novogene (Sacramento, CA) from extracted and purified RNA