title: GSM9238322: Multiplexed GEMX flex data Tumour Organoids; Mus musculus; RNA-Seq
library_name: GSM9238322
library_construction_protocol: Tumour organoids were harvested 72-hours after seeding, digested using TrpLE + Rocki and manually discociated into a single cell suspension. Tumour organoids were processed using a GEM-X Flex Sample preparation kit, before being incubated overnight at 4 degrees celcius in GEM-X flex Fixation Buffer B. Samples were washed in PBS then resuspended in GEM-X flex Quench Buffer, then dissiciated in RPMI and 5mg/ml Liberase TH. Homogenisation was then performed on a GentleMACS Octo Dissociator, cells were filtered with a 30 um strainer, pelleted and resuspended in GEM-X flex Quench buffer, then counted using equal parts of cell suspension and ReadyCount Green/Red Viability Stain on a CellDrop Automated Cell Counter. Libraries were prepared using a 10X Genomics Gem-X Flex Gene Expression kit with the Mouse Transcriptome Probe panel, according to the manufacturer's protocol