title: GSM8548255: Skin Biopsy, P, Day4, rep5; Mus musculus; RNA-Seq
library_name: GSM8548255
library_construction_protocol: Wound samples were freshly harvested and snap frozen in liquid nitrogen from mice at day 2, 4 and 12 post injury, or unwounded animals as indicated. Total RNA was extracted using TRIzol reagent (Invitrogen, Thermo Fisher Scientific), purified with chloroform, isopropanol and 75% ice cold ethanol, dissolved in RNase free water. 500 ng total RNA per sample submitted to the Center for Genomic Innovation at the University of Connecticut for library preparation (TruSeq Stranded mRNA) and sequencing on the Illumina NovaSeq 6000 platform.