title: RNA-seq of Oryzias latipes: stage 15 homozygous mutant embryo
design: DNAse tratment using TURBO DNA-free kit (Invitrogen). Ribodepletion using riboPOOL rRNA depletion, Danio rerio standard RNAseq kit (siTOOLs Biotech). Library preparation using TruSeq Stranded mRNA kit (Illumina). Chemical fragmentation. First strand sytnthesis using random primers. dTTP replaced by dUTP during second strand synthesis. A base addition on extremities. Anchor ligation. PCR amplification that also allows indexing. Library quality check using Fragment analyzer and qPCR.
library_name: s15-Mut-2