title: GSM8760664: CC030 ILC2 Vehicle Mouse 3 Day 6; Mus musculus; RNA-Seq
library_name: GSM8760664
library_construction_protocol: ILC2s were suspended in Single Cell Lysis Buffer (Takara), ILC2 were sorted into Single Cell Lysis Buffer (Takara), and subsequent library preparation and sequencing was performed at the Vanderbilt Technologies for Advanced Genomics (VANTAGE) core lab. The SMART-Seq v4 Ultra Low Input RNA Kit (Takara) was used to isolate RNA. The SMART-Seq v4 Ultra Low Input RNA Kit (Takara) was used to isolate RNA. cDNA was generated from extracted RNA through reverse transcription. cDNA was fragmented, blunt-ended, and adenylated for adaptor ligation and subsequently PCR amplified. Amplified cDNA libraries were quality controlled by bioanalyzer.