title: 16S rRNA gene metabarcoding of surface water sample: collected at Lu site on Feb_2023
design: Surface water samples were vacuum filtered using sterile 0.2 m pore size cellulose nitrate membranes until saturated. Genomic DNA was extracted from the filters using the DNeasy PowerWater Kit (Qiagen), following the manufacturer's protocol. Mechanical disruption was performed with a vortex for 15 minutes. DNA elution was carried out in 100 l nuclease-free water, and DNA samples were stored at -80 C. Additionally, a PCR quality control test was performed by amplifying the V4-V5 regions of the 16S rRNA gene with the primers Bakt-341-F (CCTACGGGNGGCWGCAG) and Bakt-805R (GACTACHVGGGTATCTAATCC). Samples were shipped to Novogene Corporation Inc. for high-throughput sequencing of the 16S rRNA V4 region on the Ilumina NovaSeq 6000 platform, generating paired reads of 250 bases each, with a minimum of 30000 reads per sample.
library_name: S36_Lu