title: RNA-seq of the chest tissue from the female adult of Phlebotomus chinensis
design: RNA was manually extracted from tissue using the Trizol method. RNA sample preparations used 1 g of RNA per sample. Libraries were created with the TruSeq RNA Kit (Illumina) following protocols, adding index codes for sample identification. mRNA was isolated using magnetic beads with poly-T oligos. cDNA synthesis involved random hexamers and M-MuLV RT for the first strand, followed by DNA Polymerase I and RNase H for the second. Exonuclease/polymerase activities made ends blunt, and Illumina Adaptors were ligated after adenylation. AMPure XP purified fragments to 350 bp, which were PCR-amplified. PCR products were cleaned with AMPure XP and quality checked on an Agilent Bioanalyzer 2100. Clustering was done on a cBot with TruSeq PE Kit (Illumina), followed by sequencing on an Illumina Hiseq X platform, generating 150 bp paired-end reads.
library_name: Phc_YQ_FT