title: gut metagenome of Homo sapiens
design: We employed the QIAamp Fast DNA Stool Mini Kit (Qiagen, Germany) to extract fecal bacterial DNA from each sample through a more advanced protocol. Subsequently, we constructed DNA libraries with approximately 500 bp insert sizes for each sample, following the Illumina TruSeq DNA Sample Prep v2 Guide (Illumina, Inc., San Diego, CA, USA). Subjecting all samples to 150 bp paired-end sequencing on an Illumina X-ten platform (San Diego, CA, USA) enabled the alignment of clean reads to the NCBI database (National Center for Biological Information, http://www.).nih.gov) for the detection of known bacteria, fungi, viruses, and archaea by SOAPaligner 2.21.
library_name: PD4E