title: 18S amplicon of saline lake protists
design: The extracted DNA was subjected to PCR using primers (Comeau et al., 2011) targeting the V4 hypervariable region of the 18S rRNA gene E572F (CYGCGGTAATTCCAGCTC) and E1009R (AYGGTATCTRATCRTCTTYG)GACTACHVGGGTATCTAATCC).
library_name: cyprus_lakes61