title: GSM9021222: Young tumor, Rep3; Mus musculus; RNA-Seq
library_name: GSM9021222
library_construction_protocol: Total RNA was isolated from tumor pieces (n=6 for young and old) using the PureLink RNA Mini Kit (Invitrogen, Cat# 12183025,) according to the manufacturer's instructions. Isolated RNAs were quality-controlled using Agilent 4200 TapeStation system (Agilent Technologies) and only preparations with RIN > 8 were used to prepare cDNA libraries. PolyA RNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and bar-coded libraries were constructed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB).