title: oxidative_stres_fecal_cultures
design: The effect of oxidative stress on the community composition of fecal microbiota cultures was addressed using 16S rRNA gene sequencing. Cell pellets underwent pre-processing utilizing the Maxwell HT 96 gDNA Blood Isolation Kit (Promega AG, Dbendorf, Switzerland) and a FastPrep homogenizer (FastPrep-24TM; MP Biomedicals, Illkirch-Graffenstaden, France) according to supplier's guidelines. Subsequently, DNA extraction was carried out using the KingFisher Flex instrument (Thermo Fisher Scientific, Waltham, Massachusetts, United States). Amplification and barcoding of the V4 hypervariable region of the 16S rRNA gene were accomplished through polymerase chain reaction (PCR) with the barcoded primer 515F (5-GTGCCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) (Integrated DNA Technologies, Leuven, Belgium)32,33. The combined library was sequenced using the amplifying primers 515F and 806R and the Illumina MiSeq platform at the Genetic Diversity Centre (ETH Zurich, Switzerland).
library_name: lib_93