title: Genome sequence of WQ 349k1
design: Genomic DNA was randomly broken by the Covaris ultrasonic crusher with fragments of approximately 350 bp. NEBNextUltra DNA Library Prep Kit for Illumina (NEB, USA) was used to construct a DNA library for sequencing. The DNA fragments were processed by terminal repair, adding A-tail, adding sequencing splice, purification and PCR amplification. Qubit 2.0 was used for preliminary quantification of the library. Agilent 2100 was used to detect the inserted fragments of the library. qPCR was used to accurately quantify the effective concentration of the library. The draft genome of strain WQ 349k1 was sequenced by using Illumina NovaSeq PE150 sequencing platform.
library_name: Tao Lu