title: Illumina sequencing of Codonopsis javanica from Vietnam
design: Total genomic DNA was extracted from fresh leaf tissue using the Isolate II Plant DNA Kit (Bioline, UK) following the manufacturers instructions. DNA quality was evaluated by 1% agarose gel electrophoresis, and concentration/purity were measured with a NanoDrop spectrophotometer (ThermoScientific, USA). Approximately 500 ng of high-quality DNA, after quality control, was subjected to acoustic fragmentation on a Covaris S220 instrument, followed by end-repair, dA-tailing, adapter ligation, purification, and size selection to generate sequencing libraries. Libraries were PCR-amplified, quantified (including qPCR to estimate effective concentration), and pooled. Library preparation, QC, cluster generation, and sequencing were performed on an Illumina NovaSeq 6000 platform to produce paired-end 150 bp reads.
library_name: TV-LHD2620_L8