title: soil fungal metagenome_ZS8
design: The purified DNA was sent to Guangdong Magigene Biotechnology Co., Ltd. (China) for high-throughput sequencing. The first PCR used ITS1-2 region universal primers to amplify the target region from the extracted DNA. A second, shorter PCR attaches full Illumina sequencing adapters, including unique dual indices (barcodes), to the amplicons from the first step. Following PCR, libraries are purified to remove primers and primers dimers, quantified, normalized to equal concentrations, and pooled into a final library for sequencing on platforms like Illumina MiSeq. After sequencing, the barcode sequences were removed, resulting in standard FASTQ files for each sample.
library_name: ZS8