title: GSM8793955: 8-week old C57BL/6J mouse lung papain+ sorted cells - GEX; Mus musculus; RNA-Seq
library_name: GSM8793955
library_construction_protocol: Eight-week-old C57BL/6J mice (species Mus musculus) were treated intranasally with 10 µg of Alexa Fluor 647-labeled cysteine protease papain in a 100 µL volume. Twenty-four hours later, lungs were processed into a single-cell suspension, and papain⁺ cells were isolated using fluorescence-activated cell sorting (FACS). Cell suspension and 10x barcoded gel beads were loaded into the 10x Chromium™ Single Cell Chip G (PN 1000120) to capture single cells in nanoliter-scale oil droplets using the 10x Genomics Chromium X Controller with Chromium Next GEM Single Cell 3' v3.1 reagents (PN-1000268). The single-cell gene expression libraries were constructed following the 10x Genomics 3' Next GEM 3.1 kit instructions. The libraries were then sequenced on an Illumina NovaSeq 6000, targeting 20,000 reads per cell. 10x Single Cell Genomics