title: V3-V4 region 16S rRNA sequencing of Homo sapiens: gut microbiota
design: DNA was extracted from fecal samples using the QIAamp PowerFecal Pro DNA Kit (Qiagen). The V3V4 region of the 16S rRNA gene was amplified using primers Bakt_341F and Bakt_805R. Sequencing was performed on the Illumina MiSeq platform, generating 2x300 bp paired-end reads with an average depth of 100,000 reads per sample.
library_name: lib_FMF_C12