title: Mutational scanning Repeat 2 positive
design: A mutational scanning library was produced based on the PADI4_3 parent sequence in which each internal position was substituted for an NNK codon. An HA-tag was added c-terminal to peptide to allow purification of translated peptides before affinity panning with PADI4. Library was subjected to one round of selection. Libraries were eluted and purified using the HA tag, indexed (Nextera XT indices) and sequenced on a MiSeq platform (Illumina) using a v3 chip as single 151 cycle reads.
library_name: Mutational_scanning_Repeat2_3pos