title: Identification of Differentially Expressed miRNAs Involved in Vascular Aging and Association with the Endocrine Hormone Regulation Pathways
design: Identification of Differentially Expressed miRNAs Involved in Vascular Aging and Association with the Endocrine Hormone Regulation Pathways
library_name: GS0276_p
library_construction_protocol: We used the data from the Namgaram project consisting of a total of 1,010 subjects at the age of 50 or above, recruited from the southern site (Jinju) in Korea, all the individuals were measured for age-related qualitative and quantitative traits, including age, grip strength, muscle strength score, and vascular endothelial functions. We randomly selected 3 samples from the top 10% and bottom 10% of RHI in each age group of 50s and 80s, and performed small RNA sequencing on a total of 12 samples. Exosomes were isolated from serum using the Exo2Dâ„¢-EV isolation kit for RNA analysis (for plasma/serurn). RNA was then isolated using the miRNeasy Serum/Plasma Advenced Kit (217204). Individual libraries were generated SMARTer smRNA-Seq for Illumina Kit (Illumina, San Diego, CA, USA)