title: GSM9059050: ATAC_MI-Ctrl-3; Homo sapiens; ATAC-seq
library_name: GSM9059050
library_construction_protocol: RNA was extracted using ReliaPrep RNA Cell miniprep system (Promega). 600 ng of total RNA was used for the construction of sequencing libraries. For ATAC-seq, Nextera Tn5 transposase was used for the tagmentation followed by chromatin purification. Library preparation was constructed using the TruSeq stranded total RNA Gold library preparation kit with RiboZero Gold treatment and unique dual indexes according to the manufacturer's instructions. For ATAC-seq, Nextera Tn5 transposase was used for the tagmentation step. The generation of 50 bp paired-end reads was performed using the SP flowcell on a NovaSeq 6000