title: GSM8793841: ATAC EYFP-PHF13-dPEST2-rep1; Homo sapiens; ATAC-seq
library_name: GSM8793841
library_construction_protocol: ATAC-seq - Following sorting, nuclei were isolated using the Nuclei EZ Prep Kit following the manufacturer's instructions for suspension cells. The isolated nuclei were stored at -80°C in RTL buffer supplemented with 2M dithiothreitol (DTT). DNA was extracted from the nuclei employing the ATACeasy Plus Mini Kit, following the manufacturer's guidelines. ATAC-seq - DNA was purified using Zymo DNA Clean & Concentrator Kit and eluted with 20 µl of elution buffer. The resulting DNA was amplified using NEBNext High-Fidelity 2x PCR Master Mix and a unique combination of Barcoded PCR Primer ( sequences provided in Buenrostro et al. 2013)