title: GSM9466888: E8.5 CD1 WT sample 1; Mus musculus; RNA-Seq
library_name: GSM9466888
library_construction_protocol: Under a dissection scope, optic vesicles (OVs) and surrounding mostly anterior tissues were collected in DMEM with 10% FBS at 4°C. Samples were organized as biological duplicates, comprised of 8 embryos (16 OVs) per replicate, per timepoint. Briefly, samples were centrifuged (4°C, 300xg), DMEM removed, and incubated with 1.0 mL of 0.25% Trypsin-EDTA at room temp for 5 mins and neutralized with FBS (10%). Samples were centrifuged (4°C, 300xg),  resuspended in 250 µl of PBS 10% FBS, and filtered through a 40 µm strainer to remove aggregates. Cell viability (>80%) was evaluated by using trypan blue and a hemocytometer. Sequencing libraries were prepared using the 10x Chromium Next GEM Single Cell 3ʹ Kit v3.1 according to the manufacturer's instructions by the Albert Einstein College of Medicine Genomics Core. Libraries were sequenced by Albert Einstein College of Medicine Epigenomics Core with the Illumina NextSeq500 at 200 million reads per sample (~20,000 reads per cell) using paired-end sequencing.