title: Antimicrobial mechanism of Lactobacillus plantarum against fish pathogens
design: Engineered DNA polymerase and 4 fluorescent labels are added dNTPs, which incorporate only a single base per cycle; Scan the surface of the plate with a laser and read the first round of each template sequence The type of nucleotide that should be polymerized; The "fluorophore" and "stopper" are chemically cleaved to restore the 3' end stickiness and continue to polymerize Combine the second nucleotide; The results of the fluorescent signal collected in each round are counted to obtain the sequence of the template DNA fragment. about Nanopore sequencing, after the library is completed, a certain concentration and volume of DNA library is added to the flow cell, and the flow cell is added Transfer to an Oxford Nanopore PromethION sequencer for real-time single-molecule sequencing
library_name: Nanopore:Illumina