title: water-supplemented diet, microbiota-depleted microbial treatment - AW2
design: Bacterial 16S rRNA gene regions were sequenced by a commercial provider (StarSeq, Mainz, Germany) on a MiSeq platform (Illumina) using double indexing and a paired end approach with a read length of 300 nucleotides. primers 341F (5- CCTACGGGNGGCWGCAG-3) and 806R (5-GACTACNVGGGTWTCTAATCC-3) were used to sequence the V3-V4 regions of the bacterial 16Ss rRNA
library_name: AW2