title: GSM8633483: CML shControl+alum-5; Mus musculus; OTHER
library_name: GSM8633483
library_construction_protocol: RNA was isolated from the transplanted mBMCs using the RNeasy plus micro kit (Qiagen 74034). Nested PCR was performed to enrich the bcr-abl kinase domain (KD) with bcr-abl specific primer set 1 (bcr-F: ACAGCATTCCGC TGACCATCAATAAG; abl-R1: ATGGTCCAGAGGATCGCTCTCT) and abl kinase domain primer set 2 (abl-F:  TGGTTCATCATCATTCAACGGTGG; abl-R2: TCCACTTCGTCTGAGATACTGGATT). The library was constructed with Nextera XT DNA Library Prep kit( Illumina, FC-131-1024  ) and purified with SPRIselect beads (Beckman Coulter, A66514). The multiplexed libraries were loaded for next-generation sequencing (NGS) on Illumina HiSeq 4000 using single-ended 50bp mode.