title: plasmodium ovale in anopheles epiroticus
design: Target DNA regions were first amplified by PCR using gene-specific primers. PCR products were purified using SPRI beads and quantified with a Qubit fluorometer. The amplicons were then subjected to library preparation using the NEBNext Ultra II DNA Library Prep Kit. Briefly, purified PCR products were end-repaired, A-tailed, and ligated to Illumina-compatible adapters. Adapter-ligated fragments were size-selected and PCR-amplified to enrich the final library.
library_name: plasm20250518B