title: GSM8686523: Donor 1, vehicle; Homo sapiens; RNA-Seq
library_name: GSM8686523
library_construction_protocol: Breast microstructures were centrifuged, washed with HBSS containing 0.1% BSA, and incubated with pre-warmed TrypLE for 15 minutes. The dissociation mixture was pipetted, followed by the addition of complete HPLM media. After centrifugation, cells were treated with RBC lysis buffer, washed, and resuspended in HPLM media with Dispase II and DNase I for further dissociation. Cells were passed through an 18G needle, filtered, and washed again before being suspended in HBSS with 0.04%BSA. All the procedures were done using Chromium Next GEM Single Cell 3' Reagent kits v3.1, dual indexing; following the 10x Genomics user guide # CG000315