title: GSM8996762: shcaf1-Hindgut-Nanopore; Drosophila melanogaster; OTHER
library_name: GSM8996762
library_construction_protocol: Genomic DNA from fly samples was extracted using Buffer A (100 mM Tris-HCl pH7.5, 100 mM EDTA, 100 mM NaCl, 0.5% SDS) and Buffer B (28.6% 5 M potassium acetate and 71.4% 6 M lithium chloride) reagents. Genomic DNA from fetal livers was extracted by using lysis buffer (100 mM Tris-HCl pH7.5, 50 mM NaCl, 5 mM EDTA, 0.2% SDS) with Protease K. Genomic DNA extracted from Drosophila hindguts was sent toBenagen for quality control and Nanopore Sequencing.