title: Mouse MHC-adapted Cryptococcus neoformans H99W
design: For RNA libraries, strains were grown in YNB to OD 1.0 and total RNA was isolated following the standard TRIzol protocol. The NEBNext Poly(A) mRNA Magnetic Isolation Module (cat. no. E7490S) was used to enrich mRNA from 1 g of total RNA. Libraries were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (cat. no. E7760S). Libraries were sequenced on an Illumina NovaSeq SP200 with paired-end reads of 100 bp for each strain. Each library consisted of roughly 1316 million sequences, with output files in fastq format.
library_name: B2_12602_RNA_3