title: GSM8861278: Liver, Downstream, Greenside Darters, biol rep 2; Etheostoma blennioides; RNA-Seq
library_name: GSM8861278
library_construction_protocol: RNA was extracted using QIAzol and chloroform (Qiagen), precipitated with isopropanol, eluted with 50μl of nuclease free water. 2 ug of totla RNA was used for the construction of sequencing libraries RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following manufacturer's protocols