title: bacterial 16S rRNA gene V1-V9 amplicon-based microbiome profile of dental plaque sample
design: Supragingival biofilms were collected using cotton swabs, transferred into RTF buffer, and disrupted by vortexing. DNA was isolated using the Fast DNA Spin Kit for Soil (MP Biomedicals Germany GmbH, Eschwege, Germany). Bacterial full-length 16S rDNA genes were amplified using the bacteria-specific primer pair 27F (AGRGTTYGATYMTGGCTCAG) and 1492R (RGYTACCTTGTTACGACTT). Preparation for sequencing, including ligation of barcoded SMRTbell Adapters, followed PacBio protocols.
library_name: ALS005__m54055_220218_122116