title: sGff_hemolymph_biol_rep2
design: Hemolymph was pooled from 35 surface-steralized sGff-positive females and stored in RNAlater. Total RNA was extracted from the PBS washed samples using the Total RNA Miniprep kit following standard protocols. Ribodepleted RNA-seq libraries were prepared by the Yale Center for Genomic Analysis and sequenced on the NovaSeq 6000 platform. Raw reads were quality filtered (Phred score >20 and read length >50 bp) and adapter-trimmed using fastp v0.24.0.
library_name: hemo2