title: RNA-Seq of Homo sapiens: A375 cell line - melanoma study
design: Cell culture and RNA extraction: The A375 cell line was cultured to a density of 80-90% in Dulbecco 's Modified Eagle Medium (DMEM) medium containing 10% fetal bovine serum.Cells were maintained at 37  C in 5% CO2 prior to collection for RNA extraction.Extraction of total RNA, including DNase I treatment to remove contamination of genomic DNA, was performed using the Qiagen RNeasy Mini Kit following the manufacturer 's protocol.Library Construction: Total RNA extracted was used to construct sequencing libraries.Library construction was performed using the Illumina TruSeq RNA Sample Preparation Kit following the manufacturer 's protocol.This process includes fragmentation of RNA, cDNA synthesis, end repair, adaptor ligation, and library amplification.After library construction, quantification and quality check of the libraries were performed using a Qubit fluorometer and an Agilent 2100 Bioanalyzer.Sequencing: The library was sequenced at both ends on the Illumina NovaSeq 6000 platform with a read length of 150 bp.Prior to sequencing, the concentration of libraries was normalized to nM grade and cluster generation and sequencing were performed as recommended by Illumina.
library_name: CA028_2