title: GSM8549445: WT-3; Mus musculus; RNA-Seq
library_name: GSM8549445
library_construction_protocol: Mice were perfused with ice cold saline and the hippocampal tissue was dissected and stored in -80 before RNA extraction. Total RNA was extracted and assessed for quality using an Agilent Tapestation 4200, and samples with an RNA Integrity Number (RIN) greater than 8.0 were used. RNA sequencing libraries were generated using the Illumina® Stranded mRNA Prep (Illumina, San Diego, CA). 500 ng of RNA was used for each sample and processed following manufacturer's instructions. libraries were multiplexed and sequenced with 100 basepair (bp) Paired End reads (PE100) to a depth of approximately 25 millionreads per sample on an Illumina NovaSeq 6000.