title: GSM9610957: GFP_pos_SIX2_plasmid_rep3; Mus musculus; RNA-Seq
library_name: GSM9610957
library_construction_protocol: A minimum of 50,000 cells, cultured in high serum-containing medium and at low density, were collected per sample for RNA extraction (Quick-RNA, Zymogen, ZR1050) following the manufacturer's protocol. Reverse transcription was performed using the SuperScript III Reverse Transcriptase (Invitrogen, 18080-093) following the manufacturer's guidelines. cDNA was generated following the SMART-Seq2 protocol. The RNA-Seq libraries were fragmented using Tn5 transposases with adaptors and amplified using PCR.