title: Transcriptional profiling of murine terminal ileum between C57BL/6 (WT) and Psen2KO mice, and Psen2KO (v2, control) and PsenDobKO mice
design: Transcriptional profiling of murine terminal ileum between C57BL/6 (WT) and Psen2KO mice, and Psen2KO (v2, control) and PsenDobKO mice
library_name: D4_6611_p
library_construction_protocol: Mice were sacrificed and its terminal ileum was collected RNA isolation Psen2/double knockout experiment: The NucleoSpinÂ® RNA Kit (Macherey Nagel) was used to isolate total RNA from tissues. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.