title: Lactiplantibacillus plantarum
design: Add modified DNA polymerase and dNTPs labeled with four fluorescent markers. Each cycle incorporates only a single base. Scan the reaction plate surface with a laser to identify the nucleotide type polymerized in the first round of each template sequence. Chemically cleave the "fluorescent group" and "termination group" to restore the 3' end sticky structure, enabling the polymerization of the second nucleotide. Compile the fluorescence signals collected in each round to determine the sequence of the template DNA fragment.For Nanopore sequencing, after library preparation, add the DNA library at a specific concentration and volume into the Flow cell, then transfer the Flow cell to the Oxford Nanopore PromethION sequencer for real-time single-molecule sequencing.
library_name: Nanopore PromethION