title: GSM9466443: Mouse PFC EAE, replicate 7; Mus musculus; RNA-Seq
library_name: GSM9466443
library_construction_protocol: The PFC of control and EAE mice were isolated and maintained in RNA-later stabilization reagent (QIAGEN). Total RNA was isolated and DNase digested using the RNAeasy Mini Kit (Qiagen) according to manufacturer's instruction. RNA libraries for RNA-seq were prepared with Universal Plus mRNA-Seq with NuQuant (Tecan) following manufacturer's instructions and sequenced using a 150 bp paired-end format on an Illumina NovaSeq 6000.