title: GSM8862111: Parental DU145 cells biological rep 2; Homo sapiens; RNA-Seq
library_name: GSM8862111
library_construction_protocol: RNA was extracted from cells using TRIzol, followed by a chloroform treatment and RNA purified using the RNA clean and concentrator kit (Zymo Research). Library preparation was performed using SMARTScribe reverse transcriptase (Takara bio) with 160 ng of RNA following the manufacturer's protocol. Library amplification was performed using Advantage 2 DNA polymerase (Takara Bio). After cDNA fragmentation, the library underwent tagmentation and purified with SPRI beads (Beckman Coulter).