title: DNA-Seq, Warri
design: Genomic DNA was extracted from the soil samples using the ZymoBIOMICS DNA Miniprep Kit. Genomic DNA samples were PCR amplified using a universal primer pair 27F and 1492R - targeting the V1 -V9 region of the bacterial 16S rRNA gene. The quality and quantity of the extracted RNA was measured using a NanoDrop (Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer). Samples were processed at Inqaba Biotechnical Industries, a commercial NGS service provider, for sequencing. Following quantification, the samples were individually indexed (NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)) for further purification.
library_name: SD1