title: GSM8603600: Plasmid Library, day0, Replicate 2, Amplicon-seq; Bos taurus; OTHER
library_name: GSM8603600
library_construction_protocol: Total DNA was extracted from 5x10^5 cells using QuickExtract reagent (Lucigen) followed by two consecutive PCR to amplify sgRNA amplicon Extracted genomic DNA was amplified and added unique sample adapters, followed by SPRI-beads size selection and a second PCR adding NGS adapters, followed by a second SPRI-beads size selection