title: GSM8792032: HGC27 cells, Treat; Homo sapiens; RNA-Seq
library_name: GSM8792032
library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. Library preparation simplifies with a kit combining double-strand synthesis, end repair, and dA-tailing. mRNA is enriched with VAHTS mRNA Capture Beads（Vazyme, China） , then fragmented, and used for double-stranded cDNA synthesis. The cDNA is then purified, and adapters are ligated. Libraries are quality-checked using the Agilent Bioanalyzer and Qubit.