title: Archaeal 16S rRNA amplicon data sampled and sequenced from the National Park of the Tablas de Daimiel (Spain)
design: DNA extraction was performed using a DNeasy PowerSoil kit (Qiagen) following manufacturer instructions. To amplify the archaeal V3-V5 region, we used the primer pair Arch349F (5-GYGCASCAGKCGMGAAW-3) and Arch915R (5-GTGCTCCCCCGCCAATTCCT-3). PCR conditions were as follow: initial denaturation at 95C for 3 minutes, followed by 28 cycles of annealing (95C for 30 seconds, 55C for 30 seconds, and 72C for 30 seconds), and a final extension at 72C for 5 minutes.
library_name: Arc_E-S_349F-915R