title: GSM9058421: BV2_norm_dmso_rep2_RNAseq; Mus musculus; RNA-Seq
library_name: GSM9058421
library_construction_protocol: Cells cultured under hypoxic or normoxic conditions, either as monocultures or co-cultures, were fixed on ice using a 3% glyoxal fixation solution, as previously described (Channathodiyil et al., PLoS One, 2021). Total RNA was isolated using the RNeasy Plus Kit (Qiagen), following the manufacturer's instructions. RNA quality was assessed using the Agilent 2100 Bioanalyzer with the RNA 6000 Pico Kit (Agilent Technologies). PolyA enriched RNA libraries were prepared with the KAPA Stranded mRNA Sample Preparation Kit (Kapa Biosystems). The RNAseq libraries were sequenced at 2x151 bp paired-end on NovaSeq 6000 at the Laboratory of Sequencing (Nencki Institute).