title: 16S rRNA sequencing of bacterial communities: tracheal aspirates from trachesotomy patients
design: FL-16S (27f - 1492r) primers were utilized for amplification. Oxford Nanopore Technologies SQK-NBD114 native barcoding kit for library preparation of the PCR products. Sequencing was performed on the GridION platform using a FLOW-MIN114 Spot-ON Flow Cell, R20 version with a translocation speed of 400bps.  Base calling was performed on the GridION using the super-accurate basecalling model with barcode trimming enabled.   MinKNOW version: 23.07.5 Guppy version: 7.0.37
library_name: 77985_NP_M_REP2_4692