title: GSM8861543: C4; Mus musculus; RNA-Seq
library_name: GSM8861543
library_construction_protocol: RNA was extracted using the Trizol method, and qualified RNA was used to construct a sequencing library. After Sample QC, the mRNA was hybridized with Oligo（dT）probe and Captured by Magnetic Beads. The mRNA was then reverse-transcripted to first strand DNA. First strand DNA was used as template to synthesis second strand DNA, obtaining dsDNA. The dTTP tailed adaptor was ligated to both ends of the dsDNA fragments. The ligation product was amplified by PCR, and circularized to get single-stranded circular(ssCir) library. The ssCir library was then amplified through rolling circle amplification (RCA) to obtain DNA nanoball(DNB).