title: Targeted T cell receptor beta chain sequencing
design: TR beta chain (TRB) gene rearrangements were amplified on genomic DNA isolated from PBMCs. In order to accurately capture the true repertoire diversity and avoid introducing biases in the analysis, the starting absolute PBMC count was standardized at 10  10 cells for all samples. Amplification of TRBV-TRBD-TRBJ gene rearrangements, library construction and sequencing were performed as previously described. A paired-end sequencing protocol was followed with the MiSeq Reagent Kit v3 (2  300 bp) on the MiSeq Benchtop Sequencer (Illumina).
library_name: RE01