title: Mitochondrial Pyruvate Carrier Inhibition Activates the Integrated Stress Response in Human Hair Follicles - RNAseq
design: Mitochondrial Pyruvate Carrier Inhibition Activates the Integrated Stress Response in Human Hair Follicles - RNAseq
library_name: Sample 5_p
library_construction_protocol: Human hair follicle samples were obtained from consenting patients from the Crown Clinic, Manchester and were handled and stores in line with HTA regulations and ethical approval.  Human hair follicles were cultured in an ex vivo organ culture model as described previously (Purba et al., 2019). Human hair follicles were subject to treatment with UK-5099 (10 and 40 µM Tocris #4186) for 48 h before isolation, embedding in frozen medium and freezing in liquid nitrogen or storage in RNA later.  Total RNA was extracted using RNeasy Micro Kit (Qiagen #74004), according to the manufacturer's protocol. Total RNA was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Quality and integrity of the RNA samples were assessed using a 2200 TapeStation (Agilent Technologies). Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.