title: ITS sequences in four different processing groups(M treatment, Rep8)
design: Sequencing libraries were constructed for fungal ITS amplicon sequencing from coal mine soil samples. The ITS1 region was amplified using the primer pair F: CTTGGT CATTTAGAGGAAGTAA and R: GCTGCGTTCTTCATCGAT. Libraries were sequenced on an Illumina platform with paired-end sequencing (data type: paired-end). Subsequent bioinformatics analysis included RDA/CCA to explore relationships between fungal community structure and environmental factors.
library_name: Fungus_M8_raw_lib