title: GSM8861868: PEO1, pLKO.1, Fulvestrant, 48h, 3; Homo sapiens; RNA-Seq
library_name: GSM8861868
library_construction_protocol: Total RNA was extracted from cells using QIAshredder columns (QIAGEN) and RNeasy Mini kit (QIAGEN), followed by DNase digestion. Libraries for RNA sequencing were prepared using the Stranded mRNAseq Library Preparation kit (Illumina, San Diego, CA) as per manufacturer's instructions starting with an input of 500 ng of RNA and 9 cycles of final PCR amplification.  Library size was assessed using the 4200 Tapestation and the High-sensitivity DNA 5000 assay (Agilent, Santa Clara, CA).