title: RNAseq data of mixed reproductive tissues (inflorescence meristems, flower buds at various developmental stages, open flowers, fruits) of Hibiscus richardsonii, forward reads
design: Whole bud, flowers, inflorescence meristems and fruit tissue were collected and flash frozen in liquid nitrogen. Per species/population, three biological replicates were collected with each biological replicate corresponding to one individual plant. Frozen samples were homogenized using a pestle and mortar and total RNA was extracted using the Spectrum Plant Total RNA Kit (Sigma STRN250). ~40 mg of tissue was used for each RNA extraction. An on-column DNase I treatment (Sigma DNASE70) was performed between the first two column washes. RNA was eluted in 30 l nuclease-free H2O (Promega MC1191) and purity was analyzed using a NanoDrop (ThermoFisher Scientific) and RNA ScreenTape (Agilent 5067-5576, 5067-5577, 5067-5578) on the Agilent TapeStation 2200 system.  Samples with minimum 1.7 g total RNA (>50 ng l-1), and A260/280 value of 2.0 were shipped on dry ice to Azenta Life Sciences, Leipzig (formerly GeneWiz) for RNA-seq library preparation and sequencing. Library preparation was performed with polyA selection and sequencing using an Illumina HiSeq machine (2x 150 bp configuration, ~350 M raw paired-end reads per lane, at least 30M reads per sample).
library_name: HRichardsonii_Forward