title: Tn-Seq of Pseudomonas aeruginosa: PA14 delta oprD
design: Bacterial DNA was extracted with QIAamp minikit (Qiagen) and digested with MmeI enzyme for 4 hours at 37C. Samples were then purified with QIAquick PCR Purification kit (Qiagen) and run on 2% agarose gel to only retrieve fragments between 1500 and 2000 bp, which were then purified with QIAquick Gel Extraction kit (Qiagen). DNA was next ligated to Illumina adapters and amplified by PCR to obtain libraries, which were then sequenced using a NextSeq500.
library_name: 1