title: GSM9024096: Huh7 cells cultured under Mix-Embed condition, replicate 2; Homo sapiens; RNA-Seq
library_name: GSM9024096
library_construction_protocol: Total RNA was extracted using TRIzol RNA isolation Reagents (Invitrogen) following the manufacturer's instructions. Messenger RNA (mRNA) was purified from total RNA using poly-T oligo-attached magnetic beads. The purified mRNA was fragmented, and first-strand cDNA synthesis was performed using random hexamer primers. Subsequently, second-strand cDNA synthesis was conducted, followed by end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Final libraries were checked for concentration and quality using Bioanalyzer 2100 (Agilent Technologies, CA, USA). Libraries that passed quality control were pooled based on effective concentration and target data output, and sequencing was performed on the Illumina NovaSeq 6000 platform using paired-end 150 bp reads.