title: GSM8826747: B16F10 cells, PEtracer mismatch kinetic screening, Day 14, gene expression library, rep 1; Mus musculus; RNA-Seq
library_name: GSM8826747
library_construction_protocol: Single cell suspensions were washed with PBS + 0.04% BSA and captured using the 10X Genomics Next GEM Single Cell 3ʹ Kit v3.1 Gene expression libraries were prepared using the 10X Genomics Next GEM Single Cell 3ʹ Kit v3.1 according to the manufacturer's instructions. Targeted lineage tracing cassette, puromycin/blasticidin barcode, and pegRNA libraries were prepared from amplified cDNA using two or three rounds of PCR with target-specific amplification primers and sample indexing primers.