title: GSM9022589: AM, GATA2 -/-, IL33, 24 hour, Rep2; Mus musculus; RNA-Seq
library_name: GSM9022589
library_construction_protocol: RNA was extracted with the Direct-zol RNA MicroPrep Kit (Zymo Research). Libraries were generated with the Illumina Stranded mRNA Library Prep kit according to the manufacturer's instructions. Following adapter ligation, 15 cycles of indexing PCR were completed, using IDT for Illumina RNA UD Indexes. Libraries were normalized and pooled to 2 nM by calculating the concentration based off the fragment size (base pairs) and the concentration of the libraries. Sequencing was performed on an Illumina NextSeq 2000, using a P3 200 flow cell. The pooled library was loaded at 750 pM and sequencing was carried out with read lengths of 2x58 bp, with a target of 25 million reads per sample.