title: GSM9466543: MCF10DCIS CD63-NanoLuC 738 Brain 28 days post-implantation replicate 2; Mus musculus; RNA-Seq
library_name: GSM9466543
library_construction_protocol: Total RNA was isolated from homogenized brain tissue collected at 3 and 28 dpi from mice implanted with MCF10DCIS CD63-NLuc cells using TRIzol reagent and purified using a Monarch Total RNA MiniPrep Kit (NEB, T2010S). Following purification,  RNA was checked for quality using the Agilent TapeStation and concentrations from ThermoFisher's NanoDrop readings were used. Stranded RNA-seq libraries were constructed using the NEBNext poly(A) mRNA isolation kit followed directly by the IDT XGen Broad Range RNA Library Prep Kit and the established protocols. Library construction was performed using 1 µg of RNA. Each library was indexed during library construction for multiplex sequencing, quantified using Invitrogen's Qubit 4 fluorometer, and checked for size and quality on Agilent's TapeStation.