title: Transcriptomics analysis of involvement of miRNA 465 in hybrid mouse sterility
design: Transcriptomics analysis of involvement of miRNA 465 in hybrid mouse sterility
library_name: juT-30_s
library_construction_protocol: Samples were obtained from testes of juvenile males, and extracted with Qiazol lysis solution  Total RNA was isolated by the Qiagen miRNeasy mini kit (217004), according to the manufacturer's protocol. For the library construction, the KAPA mRNA HyperPrep Kit with Poly(A) mRNA selection (Roche) was used with starting amount of 1000 ng of total RNA.