title: GSM8487957: Whole rosette tissue, ref4-3, 21 day, RNA, rep2; Arabidopsis thaliana; RNA-Seq
library_name: GSM8487957
library_construction_protocol: Total RNA was extracted from 21-day-old shoot tissue of 5 plants that had not bolted and that were randomly selected from 5 individual pots using a RNeasy Plant Mini Kit (Qiagen; catalog no. 74904) following with the DNase I digestion and RNA clean up included in an RNA Clean & Concentrator kit (Zymo Research; catalog no. 1014). Three biological replicates were collected for each indicated genotype. 1 μg of total RNA of each sample was used for cDNA library construction. cDNA library construction was performed using an NEBNextUltraII Directional RNA Library Prep C (NEB; catalog no. E7760S) combined with an NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB; catalog no. E7490S) according to the manufacturer's manual.