title: GSM9058789: BasalCells, E15.5, IgG, Rep2; Mus musculus; OTHER
library_name: GSM9058789
library_construction_protocol: Epcam+α6+ double positive basal cells were sorted from dorsal skin of embryos into FACS buffer (5% FBS in HBSS buffer, w/o Ca2+/Mg2+). Cells suspension was split for H3K9me3 and IgG before further processing. Then standard CUT&RUN procedure was performed. Briefly, cells were first bound to Concanavalin A beads, and then incubated with primary antibodies (αH3K9me3 or αIgG), Protein A/G MNase, underwent chromatin digestion and eventually the released DNA fragments were extracted using phenol chloroform methods. NEBNext Ultra II DNA Library Prep kit was used to prepare sequencing library, following manufacturer's instructions