title: V4 region 16S Illumina Sequences: Effect of Long-Term Azithromycin Treatment on Gut Microbial Diversity in Children and Adolescents with HIV-associated Chronic Lung Disease
design: A total of 384 barcoded libraries were processed in a single sequencing run. A two-step PCR was performed by targeting the V4 region of the 16S rRNA gene. Libraries were purified using the Agencourt Ampure beads and pooled to a normalized concentration. Pools were sequenced on the MiSeq V3 600 cycle kit using a 15% PhiX spike-in.
library_name: RS-B1027-BR29011027-Zim-BREATHE-main01-BR0027-run01-P1-D04