title: Guard cells of Chenopodium quinoa treated with salt and ABA
design: Guard cells of Chenopodium quinoa treated with salt and ABA
library_name: Cq_ABA_rep1_p
library_construction_protocol: Guard cells were enriched using the blender method (Bauer et al., 2013). Chenopodium quinoa (cv.5020) plants were cultivated in a greenhouse (at 22°C / 20°C day / night under a 16h light regime at 300 µmol m-2 s-1 white light) using common potting soil. NaCl- treatment was started after 2 weeks of growth. The NaCl concentration in the irrigation water started at 25 mM NaCl and was increased stepwise by doubling the concentration every second day up to 200 mM or 500 mM. After 5 weeks of salt treatment plants were used for experiments. For ABA stimulation of guard cells, ABA (50 µM ± ABA, Sigma) was fed to the stomata via the petiole of detached leaves, as the dense epidermal bladder growth prevented the usual spray treatment. Guard cells were enriched using the blender method (Bauer et al., 2013) and DNase I treated. Library preparation and RNAseq were carried out as described in the Illumina TruSeq Stranded mRNA Sample Preparation Guide, the Illumina NextSeq 500 System Guide (Illumina, Inc., San Diego, CA, USA), and the KAPA Library Quantification Kit - Illumina/ABI Prism User Guide (Kapa Biosystems, Inc., Woburn, MA, USA).