title: GSM9076096: iPSC-derived neurons, Day 28, XPO7+/+, replication 2; Homo sapiens; RNA-Seq
library_name: GSM9076096
library_construction_protocol: Total RNA was extracted from frozen cell pellets using the Qiagen RNeasy Plus Micro Kit following the manufacturer's instructions. RNA concentration was measured with the Qubit 2.0 Fluorometer (Life Technologies), and RNA integrity was evaluated using the Agilent TapeStation 4200. RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit (NEB) with poly(A) selection. mRNA was enriched with Oligod(T) beads and fragmented at 94°C for 15 minutes. First- and second-strand cDNA synthesis was followed by end repair, 3' adenylation, adapter ligation, and PCR enrichment. Libraries were validated using the Agilent TapeStation and quantified via Qubit and qPCR (KAPA Biosystems) before sequencing on the Illumina NovaSeq platform (2×150 bp paired-end).