title: GSM8548560: SLE-1 w and w/o IL-4_4 hrs [trans]; Homo sapiens; RNA-Seq
library_name: GSM8548560
library_construction_protocol: Single cells were captured into Gel Bead-in-EMulsions using a 10x chromium controller. Single-cell 5' biased transcriptome libraries were prepared using a 5' Library & Gel Bead Kit (PN-1000014) according to the 10x Genomics manual Single-cell mRNA was reverse transcribed into cDNA, which was then captured by a gel bead carrying barcoding DNA oligos that include a cell barcode and unique molecular identifier (UMI) attached to the VDJ region of the cDNA. The cDNA was subsequently amplified, fragmented, and constructed into a 5' biased library for RNA sequencing.