title: Treatment_2_mice_Duodenum_good
design: Male C57BL/6 mice (n=10/group) received 2 mg/kg OTA or DMSO control via gavage for 7 days after acclimatization. Analyses included histology (H&E/IHC), TEM, biochemical assays (MDA, iron, antioxidants, LPS-ELISA), RNA-seq (Illumina NovaSeq, edgeR/Limma), qRT-PCR/Western blot/IF for ferroptosis genes (e.g., SLC7A11), and 16S rRNA sequencing (V3-V4, QIIME2/SILVA) of cecal contents with diversity/correlation stats.
library_name: RNA-Treatment-5