title: GSM9138313: RNA-seq of Atf4 exon2-3 Control mice  E11.5  heart replicate 4; Mus musculus; RNA-Seq
library_name: GSM9138313
library_construction_protocol: Total RNA was extracted from cultured primary cortical neurons using the Quick-RNA Miniprep Kit (Zymo Research; Cat# R1054) following the manufacturer's instructions. RNA concentration and purity were assessed using a Nanodrop spectrophotometer. For downstream applications, equal amounts of RNA were used for either RNA-seq library preparation or reverse transcription into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Invitrogen™). RNA-seq libraries were constructed following ribosomal RNA (rRNA) depletion protocols to enrich for non-rRNA transcripts. Total RNA was subjected to rRNA depletion using a commercial RNA depletion kit according to the manufacturer's instructions. Libraries were then generated from the rRNA-depleted RNA and sequenced on an Illumina NovaSeq 6000 platform using paired-end sequencing.