title: GSM8861339: CHD7_WT_male_01; Mus musculus; RNA-Seq
library_name: GSM8861339
library_construction_protocol: Total RNA was extracted using the RNeasy kit (Qiagen), following the manufacturer's protocol. RNA libraries were prepared using the QIAseq Stranded Total RNA library Kit (Qiagen) with an input of 100 ng RNA.  Ribosomal RNA depletion was performed using the QIAseq FastSelect HMR kit (Qiagen).  RNA was then fragmented, reverse-transcribed into cDNA, and ligated with Illumina sequencing adaptors.  Size selection for libraries was performed using SPRIselect beads (Beckman Coulter), and library purity was assessed on the Tapestation 4200 (Agilent) using the High Sensitivity DNA 1000 tape, targeting a size of approximately 300 bp.