title: Effects of warming and reduced rainfall on necromass-associated bacterial communities
design: To identify the fungi associated with necromass across treatments, total genomic DNA was extracted using Qiagen DNEasy PowerSoil kits. The ITS2 rDNA subunit was PCR amplified using the dual-index barcoding approach described in Gohl et al., (2016). The fungal primer set targeting the ITS2 region was forward primer, 5.8SR (TCGATGAAGAACGCAGCG) and reverse primer, ITS4 (TCCTCCGCTTATTGATATGC). For PCR, 25 cycles were used for amplicon generation. Amplified products were magnetically cleaned using the Agencourt AMPure XP kit and quantified using a Qubit dsDNA HS Fluorometer. Each of the samples were pooled at equimolar concentration into a single library and sequenced on a Illumina NextSeq 2000 platform (2  300 bp chemistry) at the University of Minnesota Genomics Center.
library_name: F3Day14Rep1_ITS