title: GSM9467382: HEK293T GLO1 cells, Untreated_2; Homo sapiens; RNA-Seq
library_name: GSM9467382
library_construction_protocol: Total RNA was isolated from HEK293T GLO1-/- cells using the RNeasy Plus Mini Kit (Qiagen, 74134) according to the manufacturer's protocol. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA with RIN values of 10 underwent polyA selection and library preparation using mRNA Library Prep Kit (Watchmaker catalog # 7BK0001) according to instructions provided by the manufacturer with 8 cycles of PCR. Samples were barcoded and run on a NovaSeq X in a PE100 run, using the NovaSeq X 10B Reagent Kit (Illumina).