title: HiFi of Haemaphysalis longicornis
design: A single individual  was prepared to undergo DNA extraction and PacBio HiFi preparation. The sample was homogenized in a 1.5 mL tube in a liquid nitrogen bath with a liquid nitrogen-cooled pestle. The Qiagen MagAttract HMW DNA Kit (Qiagen, Hilden, Germany) was used to obtain sufficiently high-molecular weight DNA. Immediately following homogenization, 200 l 1X PBS, 20 l Proteinase K, 8 l RNase A, and 150 l Buffer AL were added to the sample. Tube was slowly inverted to mix. Sample was incubated for two hours at 25C and 900 rpm. Lysate was transferred to a 2.0 mL tube using a wide-bore pipette tip following brief centrifugation. Qiagens fresh or frozen tissue protocol was followed starting with the addition of Buffer MB.  Modifications to the protocol were as follows. All washes and rinses were incubated for 1 minute. DNA was eluted with 105 Buffer AE and incubated at room temperature for 5 minutes at 1400 rpm.
library_name: HiFi