title: GSM9650450: Male HFD paw epidermis replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Glabrous hind paw skin was harvested and incubated in Dispase (2.3 mg/ml, Sigma-Aldrich D4693) overnight at 4°C. The epidermis was gently peeled off from the dermis and incubated in TrypLE Express (Gibco 12604-013) for 10 min at 37°C. Single cells were collected in RPMI 1640 and centrifuged at 300g for 5 min at 4°C. Single-cell suspensions were loaded onto the 10x Genomics Chromium controller using the Chromium Single Cell 3' Gene Expression kit (v3.1 chemistry), targeting 5,000-8,000 cells per sample.