title: GSM8862825: 12week Wild Heart 2; Mus musculus; RNA-Seq
library_construction_protocol: total RNAs from mouse hearts at the ages of 12 weeks were extracted using the RNeasy Plus Universal Mini Kit (Qiagen). Nine representative libraries were prepared for the three groups (Wild-type n=3, Heterozygote type n=3 and Homozygote type n=3) by the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA). The sequencing libraries were clustered on a flow cell. After clustering, the flow cell was loaded on the Illumina NovaSeq instrument 6000 (Illumina, San Diego, CA, USA) according to manufacturer's instructions. The samples were sequenced using a 2x150bp Paired End (PE) configuration. RNA libraries were prepared for sequencing using standard Illumina protocols.