title: Shotgun metatranscriptomics of mixed algal-bacterial culture: day 5 of time course experiment
design: The QIAseq FastSelect 5S/16S/23S Kit (Qiagen) and the QIAseq FastSelectrRNA Plant Kit were used for simultaneous depletion of bacterial and eukaryotic rRNA. Total RNA quality was further evaluated using the Agilent 4150 TapeStation System with RNA ScreenTape reagents and chips (Agilent Technologies, Santa Clara, CA, USA).