title: GSM8656754: U2OS-Cas9 cells, Untreated, replicate B; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Sequencing libraries were generated using 2 sequential PCR reactions: the first to amplify sgRNAs from genomic DNA, and the second to add multiplexing barcodes.