title: GSM8725919: experiment  RTase, condition: Superscript 50 °C, sample 1; Mus musculus; RNA-Seq
library_construction_protocol: prime-seq - tissue lysis and homogenization in RLT+ buffer + 1 % B-Mercaptoethanol prime-seq, the prime-seq original protocol  [1] , the original protocol with adaptions and the new, improved protocol  version (Pförtner et al, this study) 1 Janjic, A., Wange, L.E., Bagnoli, J.W. et al. Prime-seq, efficient and powerful bulk RNA sequencing. Genome Biol 23, 88 (2022). https://doi.org/10.1186/s13059-022-02660-8