title: 16S metabarcoding of soil sample ERSHU271-16S-R1
design: 16S rRNA gene metabarcoding of soil samples for biodiversity assessment. Target gene: 16S rRNA. Target taxonomic group: Bacteria. Barcoding approach: two-step PCR.
library_construction_protocol: Amplicon library prepared using a two-step PCR approach. Forward primer 515F (GTGYCAGCMGCCGCGGTAA), reverse primer 926R (CCGYCAATTYMTTTRAGTTT). Primer references: https://doi.org/10.1111/1462-2920.13023; https://doi.org/10.1186/1471-2105-12-38. Sequencing performed at SciLifeLabs. Sequencing platform: Illumina NovaSeq 6000.