title: Amplicon sequencing of Influenza A barcoded virus from guinea pig nasal wash following aerosol contact with an infected animal
design: Viral RNA extraction was performed using the QiaAmp Viral RNA kit, followed by a one-step RT-PCR reaction using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity DNA Polymerase and primers targeting the barcoded region of our virus (forward: ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGAACAACACTAAACAACAGGC, reverse: GACTGGAGTTCAGACGTGTGCTCTTCCGATCTGCTTTTCCATCGTGACAACT). PCR products were purified using a column-based kit, then quantified using the Qubit dsDNA quantification kit prior to paired end sequencing.