title: GSM8656898: 56_COR-86_COR-Ago; Homo sapiens; RNA-Seq
library_construction_protocol: The library was sequenced on a NovaSeq 6000 using NovaSeq 6000 SP Reagent Kit 100 cycles (#20,027,464, Illumina) with 17*-8–105* cycle reads (* addition of one cycle according to Illumina's recommendation). The library was prepared from 10 ng of total RNA in 4 µL. The mRNA poly-A tails were tagged with universal adapters, well-specific barcodes, and unique molecular identifiers (UMIs) during template-switching reverse transcriptase (Thermo Scientific™, EP0751, Maxima H Minus Reverse Transcriptase). Barcoded cDNAs from 96 samples were then pooled and purified using a Zymo kit. cDNAs were treated with exonuclease I (NEB, M0293S) before being amplified (12 cycles) and fragmented using a transposon-fragmentation approach that enriched for 3′ ends of cDNA. The library was sequenced on a NovaSeq 6000 using NovaSeq 6000 SP Reagent Kit 100 cycles (#20,027,464, Illumina) with 17*-8–105* cycle reads (* addition of one cycle according to Illumina's recommendation).