title: 16S of Podocnemis unifilis: hatched egg
design: We extracted bacterial genomic DNA from swab samples following standard protocols for the Nucleo Spin 96 Soil extraction kit (Macherey-Nagel,Germany). We performed a two-step PCR with the primers 515 Fand 806R to sequence a 291-bp fragmen to the hypervariable V4 region of bacterial 16S rRNA and followed the Fluidigm (Acces Array Barcode Library for Illumina Sequencers-384, Single Direction, Fluidigm Corporation, South San Francisco, CA, USA) protocol for sequencing on the Illumina MiSeq sequencing platform