title: GSM8577374: SUP-B15_+PON_PCHIC; Homo sapiens; OTHER
library_construction_protocol: Cells were counted and a total of 20 million cells were harvested to be processed with the promoter-capture HiC protocol as described in Schoenfelder et al. (doi:10.3791/57320). Briefly, paraformaldehyde crosslinked nuclei were digested with HindIII, with the ends labelled with biotin and subsequently ligated. Reverse crosslink was performed and the DNA was sonicated and subsequently purified with streptavidin beads. Library construction was prepared according to the protocol described in Schoenfelder et al (doi:10.3791/57320). Briefly, xGen Stubby Adapter and UDI primer set was utilised to index each HiC library using the Phusion polymerase. Following sequencing adapter ligation, the library was purified with SPRI beads. To generate PCHiC library, biotinylated RNA probes were used to capture promoter sequences from the HiC library and amplified by PCR and subsequently purified. PCHiC library was sequenced by paired-end sequencing.