title: GSM9436333: testicular cells, low chorus at 6 hrs, biol rep 6; Pseudacris maculata; RNA-Seq
library_construction_protocol: total RNA was purified from the homogenates using NucleoZOL reagents with NucleoSpin RNA columns (Macherey-Nagel, Germany). RNA concentrations were measured with a Qubit 4 Fluorometer (Invitrogen™, Thermo Fisher Scientific, Waltham, MA, USA), and RNA integrity (RIN) was assessed using an Agilent 4150 TapeStation system (Agilent Technologies, Inc., Santa Clara, CA, USA). RNA sequencing library was performed by LC Sciences Inc. (https://lcsciences.com, Houston, TX, USA) and was prepared following Illumina's TruSeq-stranded-mRNA sample preparation protocol. Paired-ended sequencing was performed on Illumina's NovaSeq 6000 sequencing system.