title: GSM8577212: Pglyrp1-0-1; Mus musculus; RNA-Seq
library_construction_protocol: RNeasy Mini Plus kit (Qiagen) Libraries were synthesized using TruSeq Stranded mRNA sample preparation kit from 500 ng of purified total RNA and indexed adapters according to the manufacturer's protocol (Illumina). The final libraries were quantified using a Qubit fluorometer (Agilent Technologies), and RT-qPCR was performed using the Kapa Biosystems library quantification kit according to the manufacturer's protocol. Pooled libraries were subjected to 35 bp paired-end sequencing according to the manufacturer's protocol (Illumina NextSeq 500). The targeted sequencing depth was set at 30 million paired-end reads per sample