title: RNA-seq, RNA Col0 shoots chitin treated, replicate2
design: RNA-seq was performed on Arabidopsis roots and shoots of each treatment group. The shoots and roots were washed to remove soil, dried on tissue, then flash frozen in liquid nitrogen and stored at -80 C before further use. Total RNA was isolated from the target tissues using the RNeasy Maxi Kit (QIAGEN, Hilden, Germany) following the manufacturer's instructions. To study the overall transcriptional reprogramming in Arabidopsis the RNA samples per treatment were shipped to BGI Tech Solutions Co. Ltd. (Hong Kong, China) for cDNA synthesis, library preparation, and sequencing. Strand specific mRNA sequencing was performed on a DNBSEQ platform (MGI Tech Co., Ltd, Shenzen, China).