title: GSM8689038: Zbtb32; Mus musculus; ChIP-Seq
library_construction_protocol: ChIP assay was performed using Active Motif's ChIP assay kit (53035) according to manufacturer's instructions with slight modifications ChIP samples were prepared according to the Active Motif protocol. In brief, CD8+ T cells were activated with anti-CD3/CD28 for 48 h, after 24h of infection with Zbtb32-HA-GFP or Zbtb32-HA-GFP and Bcl6-RFP. Infected CD8+ T cells were purified and cultured in the presence of IL-2 (10U/mL) for another 48h. ChIP assay was performed using Active Motif's ChIP assay kit (53035) according to manufacturer's instructions with slight modifications. A total of 1 x 107 cells were lysed and digested with shearing enzyme followed by sonication. Antibody against HA tag (3724, clone C29F4; Cell Signaling Technology) was added to supernatant followed by Dynabeads Protein A (LifeTechnologies) pulling down target fragment.