title: GSM8685990: scRNA-seq of ATRIP and HC1; Homo sapiens; RNA-Seq
library_construction_protocol: PBMCs and live B cells (following FACS) were resuspended and stored pending sequencing in buffer containing PBS + 0.04% BSA. The cells were stained with TotalSeq antibodies (ADT's and HTOs). Single cells were encapsulated in Gel-Beads-in emulsion (GEMs) using the Chromium controller 3. Single-cell cDNA, FB5P (TotalSeq C ADT/HTO), BCR-enriched and TCR-enriched libraries were prepared using the SI-TT-F11 sample index with 14 PCR cycles and a final reaction volume of 35 µL. Quality control (QC) was conducted using the cDNA Bioanalyzer. The run follows the standard expectations as described by Illumina as we get 2441M raw Passed Filter (PF) reads for the full lane, while the %>=Q30 is 95% for Read 1 and 92% for Read 2.