title: GSM8577292: scRNAseq HTO 10x Lane 1; Mus musculus; OTHER
library_construction_protocol: For mammary epithelial and myoepithelial scRNA-seq, thymic IELp (CD1d-tetramer–CD4–CD8α–TCRβ+CD5+CD122+H-2Kb+PD-1+) were adoptively transferred (+IELp) or not (-IELp) into pregnant Rag2-/-c-/- /- at day 10.5 of gestation. Mammary epithelial cells and myoepithelial cells (CD45-CD31-EPCAM+) from Rag2-/-c-/- mice with or without IELp and WT were sorted 8 days post-transfer (G18.5) from samples labeled with TotalSeqC hashtags antibodies (Biolegend) with a Sony MA900 sorter Sorted cells were pooled together and 45,000 cells were loaded to a Chromium Single Cell Controller (10X Genomics) to encapsulate the cells into droplets (1 lanes). The gene expression (GEX) and hashtag oligo (HTO) sequencing libraries were prepared using a Chromium Single Cell 5' v2 reagent kits following the manufacturer's instructions and sequenced on an Illumina Nextseq2000 platform (NextSeq 1000/2000 P3 reagents (200 cycles))