title: Ms-S2 (CRX1831120; CRA027607)
library_construction_protocol: Use Oligo dT magnetic beads to enrich mRNA from total RNA. After fragmentation, use random hexamer primers to synthesize the first strand of cDNA, followed by synthesis of the second strand of cDNA. After end repair, A-tail addition, adapter ligation, fragment selection, amplification, and purification, the library is ready.