title: GSM8762137: Wwox_WT3_BM; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from Vk*MYC:Wwox WT-BM (n=3), Vk*MYC:Wwox KO-BM (n=3), and Vk*MYC:Wwox KO-PBT (n=4) samples using TRIzol reagent (Invitrogen) following the manufacturer's protocol. RNA concentration and integrity were measured on an Agilent 2100 Bioanalyzer (Agilent Technologies), and samples with RNA integrity number over 8.0 were used for sequencing. Directional mRNA-seq libraries were constructed using the ScriptSeq v2 RNA-Seq Library Preparation Kit (Epicentre). We performed 76 nt paired-end sequencing on an Illumina HiSeq3000 platform, obtaining ∼40 million tags per sample.