title: Typha minima_gen_L2_UDI332. (CRX1830454; CRA027597)
library_construction_protocol: Strand-specific mRNA sequencing libraries prepared using Illumina TruSeq Stranded mRNA LT Sample Prep Kit (polyA selection). Total RNA extracted from root tissue was enriched for polyadenylated transcripts using oligo-dT magnetic beads. Enriched mRNA was fragmented (median insert size: 350 bp), reverse-transcribed into cDNA with dUTP-based strand marking, end-repaired, A-tailed, and ligated with Illumina adapters. Libraries amplified with 15 PCR cycles. Final size selection performed using AMPure XP beads. Sequencing on NovaSeq 6000 in PE150 mode.