title: control, targeted Sequencing of ALP1in the strain with DSB and dCas9-binding between DSB and can1
design: After induction of double-strand breaks (DSB) and dCas9 binding, followed by bisulfite treatment, colonies were selected on canavanine-containing plates. These colonies were then pooled and cultured in liquid medium supplemented with canavanine. Genomic DNA was extracted from the culture and used as a template for PCR amplification of the CAN1 or ALP1 genes. The resulting PCR products were subjected to paired-end sequencing to determine the mutation profiles.