title: Heat Shock HEK293 R4
design: HEK293 (1 Mio. cells) were plated on petri dishes (Greiner, diameter 10 cm, REF: 664160). After 48 h, plates were sealed with Parafilm and submerged in a water bath. Heat shock treatment was 42 C for 45 min. Plates were put back at 37 C to let the cells recover from heat shock. Non-induced cells were maintained at 37 C.