title: GSM9437074: Fast16h, wild type mouse liver, isolated pericentral (PC) hepatocytes, RNAPII (ChIP); Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes at room temperature and quenched with glycine. Chromatin was sonicated using Bioruptor Pico device (Diagenode). ChIP-seq libraries were prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, #E7645) with NEBNext® Multiplex Oligos for Illumina® single Index Primers Set 1 and Set 2 (New England Biolabs, #E7335 and #E7500)