title: GSM8577695: brain_5; Mus musculus; RNA-Seq
library_construction_protocol: Tissue was obtained from mouse brains by shaving with a razor blade. The resulting tissue was washed in PBS and dissociated into single cells using the Adult Brain Dissociation Kit. RNA-seq library was constructed using a modified sciRNA-seq protocol. The target cells were sorted into 384-well plates with barcoded oligo dT primer for each well (1 cell / well). After reverse transcription in each well, cDNA was pre-amplified using the xGen™ ssDNA & Low-Input DNA Prep (IDT). Purified cDNAs were tagmentationed with Amplicon Tagment enzyme (Illumina) and PCR for library construction was done by using NEBNext High-Fidelity 2× PCR Master Mix (NEB).