title: RNA-Seq of zebrafish: B355
design: RNA was isolated and purified by the RNeasy Mini Kit (Qiagen, Germany) with three replicates per group. Total RNA quality was assessed using RNA agarose gel electrophoresis, and RNA quantity was determined using a spectrofluorometer (MySpec, VWR, Radnor, United States). Complementary DNA (cDNA) and the generated libraries were prepared according to the manufacture protocol and sequenced using the Illumina NovaSeq 6000sequencing platforms at the University of Southern Denmark.