title: GSM9239673: Alpha-beta  neurons, 24 hours after pupa formation, rep 5; Drosophila melanogaster; RNA-Seq
library_construction_protocol: Brains were dissected a cold Ringer's solution, incubated with collagenase/dispase mix at 29ºC, washed in dissociation solution, mechanically dissociated into single cells and transferred via 35um mesh. 1000 GFP+ positive cells were sorted directly into 100μl Pico-Pure RNA isolation kit extraction buffer followed by RNA extraction using the kit or stored in -80 for later use. Libraries were prepared based on the smart-seq2 protocol (Picelli et al., 2014). Briefly, Reverse transcription was performed using Oligo-dT primers, followed by PCR.The amplified cDNA underwent library generation using Nextera XT (Illumina)