title: GSM9538385: MDA-MB-231 cells, CTRV2_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from control and RNASEH2A-deficient MDA-MB-231 cells were extracted using a Total RNA Kit I (RNeasy Kit, Qiagen). RNA from three different passages per group was extracted according to the kit manufacturer's instructions. RNA purity and concentration were assessed using a NanoDrop spectrophotometer (Thermo Scientific), and RNA integrity was evaluated using the Agilent 2100 Bioanalyzer system (Agilent Technologies). Samples with RNA integrity number (RIN) ≥ 7.0 and A260/A280 ratio between 1.8–2.1 were used for library preparation. Strand-specific mRNA library constructed using the poly-A enrichment method.