title: GSM8513844: PR8 immune P14 replicate A, GEX; Mus musculus; OTHER
library_construction_protocol: After enzymatic and mechanical tissue diggestions, enriched lymphocyte cell suspensions were stained with Live/Dead dye (Ghost-Dye 510, Tonbo), fluorescent-conjugated antibodies (CD8b (RPA-T8), Thy1.1 (HIS51), CD45.1 (A20), Thy1.2 (30-H12)), and oligonucleotide-conjugated Biolegend TotalSeq-A antibodies (30 min at 4ºC ). P14 (Live, CD8a+, Thy1.1+ and CD45.1+) P14 T cells were sorted using a FACS Aria II (BD Biosciences). We followed the 10X Genomics Chromium Single Cell 3' v3 protocol to prepare RNA, antibody-derived-tag (ADT), and hashtag oligos (HTO) libraries.