title: Jute wastewater_500 - T1:R1
design: Genomic DNA was quantified using a Qubit 4.0 fluorometer. The V3V4 region (~459 bp) of the 16S rRNA gene was amplified using specific primers (V3-F and V4-R) with Illumina overhang adapter sequences. Amplicons were purified with AMPure XP beads, followed by index PCR using the Nextera XT Index Kit. Indexed libraries were purified with AMPure XP beads and quality-checked on an Agilent TapeStation 4150 using High Sensitivity D1000 ScreenTape. Final library concentration was measured by Qubit HS and qPCR before pooling. Sequencing was performed on the Illumina MiSeq platform using a paired-end 300 bp (PE300) kit.