title: GSM8761487: SW1990 biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the NucleoSpin® RNA Plus kit. 150 ng of RNA was processed through the Watchmaker RNA Library Prep Kit with Polaris™ Depletion, version v1.1.1122. The following modifications were made : (1) library prep reagents were reduced to 50%, (2) fragmentation was performed for intact/high quality RNA (fragmentation for 5 minutes at 85°C), (3) the optional 2nd post-ligation cleanup was performed and (4) libraries were amplified using 14 PCR cycles.