title: GSM9263578: RNA_TotalNK_NK11_6hr_3_IGO_12001_19; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq: RNA was isolated from sorted cells and/or stimulated cells as described using PicoPure RNA Isolation Kit (ThermoFisher Scientific, cat. KIT0214). After RiboGreen quantification and quality control by Agilent BioAnalyzer. For ATAC-seq: ATAC-seq was performed as described previously. Briefly, sorted, stimulated cells were washed in cold PBS and lysed. The transposition reaction was incubated at 37 °C for 30 min. DNA was cleaned with the MinElute PCR Purification kit (QIAGEN, cat. 28004). For RNA-seq: RNA amplified using SMART-Seq v.4 Ultra Low Input RNA kit (Clontech, cat. 63488). Subsequently, amplified complementary DNA was used to prepare libraries with KAPA HyperPrep Kit (Kapa Biosystems, cat. KK8504). Samples were barcoded and ran on NovaSeq6000 in a 100bp/100bp paired-end run. For ATAC-seq: DNA materials were amplified and the final product was cleaned by AMPure XP beads (Beckman Coulter catalog no. A63882) at a 1× ratio, and size selection was performed at a 0.5× ratio. Libraries were sequenced on a NovaSeq6000 in a 100 bp/100 bp paired-end run, using the NovaSeq6000 S2 or S4 Reagent kit (Illumina).