title: RNAseq data for phs004321
design: Total RNA was isolated from each hNPC line. For each experimental week, hNPCs were lifted with Accutase (Thermo Fisher Scientific A1110501), lysed, and stored in TriZol (Thermo 15-596-026) at -80C for subsequent purification of total RNA using RNeasy Mini Kits and on-column DNase digestion (Qiagen 74106, 79256). We quantified RNA isolates using fluorescence-based Quant-iT RNA assay kits (Thermo Q33140). Mean RNA integrity number (RIN) across all samples was 9.86 (SD = 0.39). Library preparation was performed using a stranded total RNA kit (KAPA KR0934) after samples were depleted of ribosomal RNA (KAPA KR1151). RNA-seq libraries were sequenced at the NYGC to an average sequencing depth of 55M (SD = 13M) paired-end reads (2x100bp) per sample on the Illumina Novaseq platform.