title: GSM8577309: HD-C_Pos; Homo sapiens; RNA-Seq
library_construction_protocol: B cells were isolated from frozen PBMC via 3 different methods: positive MACS (EasySep Human CD19 Positive Selection Kit II; StemCell Tech), negative MACS (EasySep Human B cell Isolation kit; StemCell Tech), and FACS (sorted Live/CD19+ cells; MACSQuant Tyto Cell Sorter, Miltenyi). RNA was isolated via the RNeasy Plus Micro kit (Qiagen). Libraries were prepared using the TruSeq Stranded mRNA library prep kit (Illumina) following manufacturer's protocol.