title: GSM8689926: U2OS cells, cisplatin, Pol I (ChIP), rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% paraformaldehyde in phosphate buffered saline for 10 min at RT, then quenched with 125mM glycine. Fixed cells were lysed in RIPA buffer on ice, and chromatin was sheared using a Diagenode Bioruptor sonicator. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit, for Illumina (New England Biolabs, #E7600S) and NEBNext Ultra II DNA Library Prep Kit, for Illumina (New England Biolabs, #E7645S). 10 ng of DNA was used for input samples and for 1 ng of DNA was used IP sample. Libraries were amplified using 12 cycles on a thermocycler. Post amplification libraries were size selected at 200-400 bp in length using Omega Mag-Bind TotalPure NGS beads. Libraries were validated on an fragment analyzer using an Agilent NGS Fragment kit.