title: GSM8547964: fetal fetus, E14.5, BulkRNA-seq, RORgt_LTi_4; Mus musculus; RNA-Seq
library_construction_protocol: E14.5 fetal livers were separated under a microscope, and single-cell suspension was generated by pipetting with a 1000-micropipette. The cell suspension  was then filtered through a 100 μm strainer and resuspended in FACS buffer (2% FBS/0.05% NaN3/1% PBS). Bulk RNA-seq was performed on 50-200 sorted cells using the SMART-Seq HT PLUS Kit following the manufacturer's instructions (Takara Bio).