title: fecal sample
design: Full-length 16S rRNA sequencing was conducted at Maintain Biotech (Wuhan, China). Fecal sample collection, and DNA extraction were described as previous described 24. The full-length 16S rDNA (V1-V9 regions) of bacteria was PCR amplified using the universal bacterial primers 27F 5-AGAGTTTGATCCTGGCTCAG-3 and 1492R 5-GGTTACCTTGTTACGACTTO-3. The raw FAST5 data of full-length 16S sequences from Nanopore sequencing was processed using the PacBio SMRT portal (PacBio, Menlo Park, CA, USA) for initial analysis. Amplicons were trimmed on the PacBio platform to discard sequences outside the expected amplicon size range. Taxonomic classification was then assigned based on the Mothur algorithm, referencing the SSUrRNA Database of Silva Database (Version 138.1).