title: GSM8514416: Salt_RNASeq; Mesembryanthemum crystallinum; RNA-Seq
library_construction_protocol: Total RNA was extracted from 15 seedlings using 1 mL TRIzol reagent (Invitrogen) according to the manufacturer's instruction. The library construction involved several steps: initially, mRNA was isolated from total RNA using magnetic beads with poly-T oligonucleotides to target poly-A containing mRNA, followed by heating to fragment the isolated mRNA. This was then reverse transcribed into cDNA using random primers. Adaptors were then attached to both the 3' and 5' ends, followed by PCR to amplify the fragments. The PCR-amplified cDNA was assessed with both Experion™ and the Qubit™ 3.0 fluorometer (Invitrogen™, U.S.A.) to ensure that the fragment length and concentration met the requirements for the Illumina® Hiseq 2000 sequencing platform.