title: WRKY03 DAPseq of Vitis vinifera: WRKY03
design: Genomic DNA was extracted from 1 g of ground young leaves of cv. 'Cabernet franc' and Illumina libraries were prepared as previously described by Galli et al. (2018). The VvWRKY3 open reading frame (ORF) was selected based on polymorphism and allelic data from 123 V. vinifera cultivars, identifying the most representative allele (Magris et al., 2021). The sequence was used to prepare synthetic constructs (Thermo Fisher Scientific) and inserted into the pDONR221 vector. VvWRKY3 ORFs was transferred to the Gateway-compatible pIX-HALO destination vector tagged at the N-term via LR recombination (Bartlett et al., 2017), with a pIX-HALO vector containing the GST sequence serving as a negative control. Successful integration of VviWRKY ORFs was confirmed by diagnostic restriction analysis using XhoI (Thermo Fisher Scientific).