title: HIC-Seq of Gibbotettix parvipulvillus: adult male specimen
design: Hi-C libraries were prepared from an adult male Gibbotettix parvipulvillus specimen following an in situ Hi-C protocol with the restriction enzyme Mbo I. Briefly, chromatin was cross-linked with formaldehyde, digested with the restriction enzyme, and proximity-ligated to create chimeric molecules representing 3D genomic interactions. After reverse cross-linking, DNA was purified, fragmented, and prepared for paired-end sequencing (2150 bp) on the BGISEQ-500 platform.