title: GSM8761311: HFD Zzef1f/f-RipCre+, replicate 1, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Purified mouse islets were treated with trypsin-EDTA (0.05%) at 37 ℃ for 4 min. and gently pipetted. The cell suspension was stained with 0.4% trypan blue to assess cell viability under microscopic observation. Cells with greater than 85% viability were qualified for library construction process. Three mice islet samples (βZKO-Mip and Mip-Cre mice, βZKO-Rip and RIP-Cre mice) were used for one biological capture, and all observations and insights in the single cell analyses derived from two biological replicate per genotype. All sequencing date were captured with two independent experiments. The single-cell suspension of islets was loaded into the 10X Genomics Chromium Controller (10x 3' Genomics, USA) for encapsulation, barcoding, and cDNA synthesis, following the manufacturer's protocol