title: WES-Seq of tumor adjacent tissue from P1017343
design: Genomic DNA was extracted from tumor and tumor-adjacent tissues using a commercial kit (TIANGEN Biotech, DP341) following the manufacturer's instructions. DNA quality and concentration were assessed by Nanodrop OneC and Qubit 3.0 fluorometer. DNA integrity was verified by 0.5% agarose gel electrophoresis. DNA libraries were prepared from 50200 ng of high-quality genomic DNA. Exome regions were captured using the xGen Exome Hyb Panel v2 (IDT). Captured libraries were PCR amplified, size-selected (200500 bp), and quantified prior to sequencing on the DNBSEQ-T7 platform (MGI) using 150 bp paired-end reads.