title: GSM8577721: WT female Lung, IL-33, 4 days,  ATAC-seq, replicate1; Mus musculus; ATAC-seq
library_construction_protocol: Female mice were used for library construction unless otherwise stated. Lung ILC2s were purified from IL-33-treated Il5Cre/+Smarca4+/+(Ctrl) and Il5Cre/+Smarca4f/f (KO) mice. Mice were intraperitoneally injected with 500 ng recombinant mouse rmIL-33 (BioLegend) in 100 μl PBS for 4 consecutive days. On day 5, lungs were harvested. Each biological repeat was pooled about 5×104cells from 2-4 mice. Library was constructed by TruePrepTM DNA Library Prep Kit V2 for Illumina® kit from Vanzyme (Nanjing, China). 1.2×Agencourt AMPure XP beads (Beckman Coulter) were used to purify the libraries.