title: GSM8943285: AB18634; Mus musculus; RNA-Seq
library_construction_protocol: Dorsal and ventral sides of the mouse ears were manually separated and cut into small pieces and digested in serum-free Dulbecco's modified Eagle's medium (DMEM, Sigma-Aldrich) containing Liberase TL (Thermolysin Low) Research Grade (0.25 mg/ml, Roche) for 75 min at 37°C. Digested tissue was smashed and filtered through a 70-µm cell strainer to obtain single-cell suspensions. Afterwards, cells were washed and resuspended in FACS buffer (PBS, 0.5% BSA and 2 mM EDTA) and prepared for flow cytometry surface marker staining and single-cell sort. 3' end mRNA libraries were prepared for sequencing using SPID-seq (Yofe et al, Cancer Discovery 2023) Library are single end library. R2 contain cell barcode and UMI only.