title: GSM9308034: RNA-seq ADCV, chromatin fraction, Adult 30m opto to 24hr, rep5; Mus musculus; RNA-Seq
library_construction_protocol: For NEUROD1/2 knockout mice, cerebella were removed, microdissected, and total RNA from ADCV was extracted with Trizol. For optostimulation, the medial part of the ADCV was homogenized and spun down. The supernatant was removed and the RNA in the pellet representing the chromatin fraction was extracted with Trizol. Purified RNA was treated with NEBNext rRNA depletion kit  (#E6310). RNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit (#E7760).