title: GSM8689086: RS_HS_MCF7_siCTL; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNeasy Mini Kit (Qiagen) following the manufacturer's protocol. DNase I in column digestion was included to ensure the RNA quality. RNA library preparation was performed by using NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina (E7420L) and paired-end sequencing was performed with Illumina HiSeq 4000 platform at RiboBio Co., Ltd. or Amogene Biotech Co., Ltd.