title: JZ-D6
design: Genomic DNA was extracted from each sample of rumen content using a FastDNA SPIN for Soil Kit (MP Biomedicals, Solon, USA), following the manufacturer's instructions. The quality and quantity of the extracted DNA were assessed using a NanoDrop spectrophotometer 2000 (Thermo Fisher Scientific, USA) and 1% agarose gel electrophoresis. Samples with a 1.8 absorbance ratio (260/280 nm) and a clear band on the gel were used for further analysis. DNA libraries were prepared using the Illumina TruSeq DNA Library Preparation Kit according to the manufacturer's protocol. Briefly, the DNA was fragmented using a Covaris S2 Focused Ultrasonicator (Thermo Fisher Scientific, USA) to achieve an average size of approximately 300500 bp. Then, the fragmented DNA was end-repaired and ligated with sequencing adapters. The libraries were then size-selected and PCR-amplified. The quality of the libraries was assessed using an Agilent Bioanalyzer 2100(Agilent Technologies, Inc., Santa Clara, USA), and the libraries were quantified using qPCR. Sequencing was performed on the Illumina HiSeq platform (HiSeq X-10, Beijing, China) using a paired-end 150 bp (PE150) strategy.