title: TCR-Seq of PBMC from P980589
design: Total RNA was extracted from peripheral blood using the PAXgene Blood RNA Kit (Qiagen, Cat. No. 762174) according to the manufacturer's instructions. After RNA extraction, genomic DNA was removed using DNase I (Thermo Scientific, Cat. No. EN0521). RNA quantity and purity were assessed using a Nanodrop OneC spectrophotometer, and RNA integrity was evaluated via 1.5% agarose gel electrophoresis. RNA concentration was further quantified using a Qubit 3 fluorometer with the Qubit RNA HS Assay Kit.Approximately 2 g of total RNA from each sample was used for TCR repertoire sequencing. Libraries were prepared using the KC-Digital Stranded TCR-seq Library Prep Kit for Illumina 150 (Seqhealth Technology Co., Ltd., Cat. No. DT0813-02), which employs unique molecular identifiers (UMIs) with 8 random bases to eliminate PCR and sequencing duplication bias. The resulting library fragments (250500 bp) were enriched, purified, and quantified. Sequencing was performed on an Illumina NovaSeq system using paired-end 150 bp (PE150) mode.