title: GSM8577445: WildType_2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from HEK293T and LEDGF KO cells with RNeasy Plus Mini kit (Qiagen) following manufacturer's instructions. Approximately 1 µg of total RNA per sample was purified with PolyA extraction, and then purified mRNAs were used to make RNA-Seq libraries with specific barcodes using Illumina TruSeq Stranded mRNA Library Prep Kit. All the RNA-Seq libraries were pooled together and sequenced using illumina NovaSeq 6000 to generate approximately ∼40 million 2×100 paired-end reads for each sample. The raw data were demultiplexed and analyzed further.