title: GSM8944005: SCC-25, RARG KO, H3K27me3, biol rep 1; Homo sapiens; OTHER
library_construction_protocol: SCC-25 cells were fixed the next day for 2 minutes with 0.1% methanol-free formaldehyde directly in the dish before quenching with 20x Glycine for 2 minutes. Cells were trypsinized and collected, washed with 1% FCS in 1XPBS, and resuspended in Nuclei Extraction Buffer (Epicypher) for 10 minutes on ice.400,000 nuclei per sample were added to activated ConA beads and processed according to  Epicypher's CUTANA Cross-linking Protocol (v1.9, 2022). ). SNAP-CUTANA™ K-MetStat Panel of d-nucleosomes was spiked into IgG, H3K4me3, and H3K27me3 samples. E. coli DNA was spiked into all samples for single scalar normalization. IDT-xGen kit