title: 16S rRNA V3 amplicon sequencing of cloacal and oral microbiota from Crocodylus acutus
design: The library preparation was performed by amplifying the V3 region of the 16S rRNA gene with the primers 16S-V3_338f and 16S-V3_533r, following a standard protocol to obtain ~196 bp amplicons. Real-time PCR was performed with Nextera XT indexes and specific amplification conditions. The products were visualized by gel electrophoresis, quantified, purified with AMPure XP magnetic beads, normalized to 4 nM, and sequenced on a MiniSeq platform (Illumina) with paired-end (2  150 bp) reads.