title: GSM8513882: Isogenic control, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from MN cultures using the miRNeasy micro kit (Qiagen; Cat#217004) with DNase treatment (Qiagen; Cat#79256) following the manufacturer's instructions. Samples with RNA integrity numbers above 6.5 determined by a Bioanalyzer (Agilent) were used for library preparation. RNA libraries were prepared using the NEB mRNA stranded library preparation kit  following the manufacturer's instructions.