title: ONT sequencing: Piscinibacterium candidicorallinum LMG 29480
design: Piscinibacterium candidicorallinum LMG 29480 was obtained from the BCCM/LMG culture collection (Ghent, Belgium). Cells grown aerobically in  R2A broth were harvested by centrifugation (3000g, 4C, 30 min), followed by DNA extraction using a GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). Library preparation was performed with 1.5g high-molecular-weight DNA and an Oxford Nanopore Technologies (ONT) ligation sequencing kit (SQK-LSK114). Intentional shearing and size selection were not performed prior to library preparation. The library was sequenced on an ONT MinION Mk1C device with an R10.4.1 flow cell. Dorado v 7.4.12 was used for base calling using the SUP model and adapter/barcode removal.