title: Intestinal tissue sample was processed within 3 hours of collection with epithelial stripping followed by collagenase digestion to generate a single cell suspension and submitted to single cell RNA sequencing.
design: Cells were loaded on a 10x Chromium controller and processed following the NextGEM v3.1 single-index 3' Chemistry (10x Genomics, CG000204_ChromiumNextGEMSingleCell3'v3.1_Rev D). Libraries were sequenced on an Illumina HiSeq with the following read configuration: R1 (cell barcode and UMI): 28bp, i7 index: 8bp, R2 (insert): 96bp.