title: RNA-Seq of 84K poplar
design: Total RNA was initially extracted from the leaves of 84K poplar. The concentration and purity of the RNA were measured using a Nanodrop 2000 spectrophotometer, while RNA integrity was assessed by agarose gel electrophoresis and the RQN value was determined using an Agilent 5300 system. mRNA was enriched from the total RNA using magnetic beads with Oligo (dT), then randomly fragmented into approximately 300 bp segments. These fragments were reverse-transcribed to synthesize cDNA, followed by end repair, A-tailing, and adapter ligation.