title: GeCKO-B sgRNA library
design: Original GeCKO-Blasticidin sgRNA library used for the screen. The 190bp library for sequencing was generated by PCR from the maxipreped lentiCRISPR library using primers GF4 (5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNTGTTCTTGTGGAAAGGACGAAACACCG-3) and GRfixed (5-CAAGCAGAAGACGGCATACGagatACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAAC-3).