title: GSM8689803: Akt_Notch OE rep 2; Mus musculus; RNA-Seq
library_construction_protocol: After one week of overexpression or at humane endpoint, primary mouse liver tumor samples were dissected and placed into TRIzol (Invitrogen). Samples were crushed and then homogenized using QIAshredder (Qiagen). For bulk RNA sequencing, libraries were prepared according to the dUTP protocol and paired-end sequencing (2x100bp) was performed on the NovaSeq 6000 platform (Illumina).