title: Allele frequency changs in temporal populations of two timothy cultivars
design: Allele frequency changs in temporal populations of two timothy cultivars
library_construction_protocol: For population genetic studies, approximately 80–100 seeds of each population were sown in two pots and cultivated at ambient room temperatures for 8 weeks. For DNA extraction, approximately an equal amount of tissue from 30 randomly selected plants from each pot was collected and flash-frozen in liquid nitrogen. Plants were grown for 8 weeks at ambient room temperature in green houses DNA extractions were carried out using Promega wizard HMW DNA extraction kit according to the manufacturer's guidelines restriction enzymes - RsaI and HaeIII were choosen to uniformly cover the genome based on pre-experiment 