title: Polar amino acids amended culture 1
design: DNA was shipped to Molecular Research DNA Lab (Texas, USA) for sequencing using the updated Earth Microbiome Project (EMP) 16S rRNA primer set91,92: 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3).