title: GSM8761320: A8_Donor02_NonNeut; Homo sapiens; RNA-Seq
library_construction_protocol: To prepare lymph node mononuclear cells (LNMCs) for 10x single-cell RNA sequencing, lymph node tissue was mechanically and enzymatically dissociating to obtain a single-cell suspension. Next, mononuclear cells were isolated using density gradient centrifugation, the cells were filtered using 10x's protocol and highly viable cells were used for single-cell RNA-sequencing. Library preparation was performed following the manufacturer's instructions (10x Genomics, Single Cell 3' v3 protocol). Briefly, cells were resuspended in the master mix and loaded with partitioning oil and gel beads into the microfluidic chip to form gel bead-in-emulsion (GEM) droplets. Poly-A RNA from cell lysates within each GEM was reverse transcribed into cDNA. Illumina's paired-end  library constructs containing P5 and P7 sequences, along with a sample index, were generated and sequenced on BGISEQ 500.