title: Whole-metagenome sequencing of wastewater effluent: F.LL WWTP, Week 1
design: Water samples were collected in sterile 2 L glass containers, maintained at 4C, and filtered within 24 h of arrival at the laboratory. Filtration was performed through 0.22-m nylon membrane filters (Whatman, Maidstone, UK) using a filtration device and a vacuum pump until membrane saturation, and the filters were stored at 20C until DNA extraction. Extraction of DNA from filters was performed using the DNeasy PowerSoil kit (Qiagen Laboratories, Inc.) according to the manufacturer's instructions. The concentration and the quality of the DNA was tested using a NanoDrop Spectrophotometer 8000 (Thermofisher Scientific, Inc), and all of the DNA extracts were stored at 20C until DNA library preparation. DNA samples were sent to Novogene UK for DNA library preparation with the NEBNext Ultra IIDNA Library Prep Kit (Cat No. E7645; New England Biolabs), and for paired-end 150 bp sequencing on a single lane on a NovaSeq X Plus Series platform (Illumina) using the 25B flow cell.