title: FSC3_1
design: RNA was extracted using PureLink RNA Kit (Ambion, USA, Cat # 12183018A). Five PCR-positive individual T1 plants, representing the selected transgenic family, were used, and sesame capsules were harvested at different developmental stages for RNA isolation, three biological replicas, for each developmental stage. Similarly, five non-transgenic plants were used as capsule sources and pooled together for RNA isolation from false septs tissues (four developmental stages). The RNA was treated with Turbo DNA-free (Ambion, USA, cat #AM1907). RNA quality and concentration were determined using Experion Automated Electrophoresis System by Bio-Rad (Cat No. 7007010). The integrity of RNA was assessed using an Agilent 2100 bioanalyze. The samples were prepared, and BGI-China constructed and synthesized the cDNA library.