title: GSM9686850: Patient15,PBMC,conB1(RNA-seq); Homo sapiens; RNA-Seq
library_construction_protocol: RNA-seq:Total RNA was extracted using TRIzol reagent according to the manufacturer's instructions. RNA quality and integrity were assessed using an Agilent 2100 Bioanalyzer. ChIP-seq:Cells were crosslinked with 1% formaldehyde for 10 min and quenched with 125 mM glycine. Chromatin was isolated, lysed, and sonicated to generate DNA fragments of 200–500 bp. RNA-seq:RNA-seq libraries were prepared using a poly(A) selection method with the Illumina TruSeq RNA Library Preparation Kit according to the manufacturer's instructions. ChIP-seq:Immunoprecipitated DNA was collected using magnetic beads and used for library construction with the NEBNext Ultra II DNA Library Prep Kit according to the manufacturer's instructions.