title: RNAseq of homo sapiens ureteric urothelium: BKPyV infected
design: Ureteric urothelial cells were isolated from excess ureteric tissue following renal transplant. Urothelial cells were dissociated from underlying stroma, expanded and split before biomimetic re-differentiation. Cell cultures were maintained to generate a tight barrier as measured by TEER. Half of replicate cultures were then infected with BKPyV and maintained to 21 days post infection when RNA was extracted using TRIzol. Matched control mock cultures were also harvested at the 21 day timepoint.