title: AJU1c metagenome- Fungi
design: Amplicon sequencing of bacterial, archaeal, and fungal communities using Oxford Nanopore Technologies (ONT). PCR amplicons were purified using AMPure XP beads and barcoded using the ONT Native Barcoding Kit. Libraries were prepared with the SQK-LSK109 ligation kit and sequenced on a MinION Mk1B platform.