title: WGS of Cardamine amara STE_H
design: Genomic DNA was extracted from silica-gel-dried leaves using sorbitol extraction method and then purified using AMPure XP (Beckman Coulter Inc., Brea, California, USA).  Samples were genotyped for genome-wide single nucleotide polymorphisms (SNPs) using the double digest RADseq protocol of Arnold et al. 2015 with modifications (see Knotek et al., 2020). The libraries were sequenced with 300 cycles (2150 bp paired-end/PE reads) using the Illumina HiSeq platform.