title: GSM9538425: NUGC4,ATAC,KO,rep2; Homo sapiens; ATAC-seq
library_construction_protocol: Approximately 100,000 cells were isolated and permeabilized. The permeabilized nuclei were resuspended in 50 µl tagmentation buffer (33 mM Tris-Ac, 66 mM K-Ac, 11 mM Mg-Ac, 16% DMF, pH 7.8) with 1 µl Tn5 transposase and incubated at 37°C for 30 min. Fragmented DNA was purified using QIAquick PCR Purification Kit (Qiagen, #28106). Purified tagmented DNA was amplified using NEBNext High-Fidelity 2X PCR Master Mix (NEB, #M0541L). Libraries were purified, quality-checked, and size-selected. Paired-end 150 bp sequencing was performed on an Illumina NovaSeq X Plus platform.