title: RNA-Seq of rat: lung
design: For high group replication 1, rats were anesthetized and fixed in the supine position, a rat-specific laryngoscope was inserted, and a pre-prepared 50 l PM2.5 suspension (PM2.5-80 mg/ml) was slowly injected. The rats were exposed every two days for a total of 12 exposures. At the end of the experiment, the lungs of the rats were removed for miRNA transcriptome analysis.