title: Genome resequencing of the Scaly-foot Snail: Adult foot tissue
design: For DNA extraction, the foot tissues (50-100 mg) were thawed and extracted using DNeasy Blood & Tissue Kits (Qiagen, Germany). DNA quality and integrity were assessed via NanoDrop spectrophotometry, agarose gel electrophoresis, and Qubit fluorometry. Sequencing libraries (350 bp insert size) were prepared from purified DNA using the NEBNext Ultra II DNA Library Prep Kit for sequencing on the Illumina NovaSeq 6000 platform (paired-end 150 bp reads).