title: RNA-seq of mouse Th17
design: rRNA depletion was performed using Ribo-Zero Human/rat/mice Ribo-Zero plus rRNA Depletion Kit (Illumina). Library preparation was performed using 1 g of rRNA-depleted total RNA using Truseq Stranded Total RNA LT Sample Prep Gold kit, without molecular barcodes. Sample preparation followed preparation using the NovaSeq 6000 S4 Reagent Kit (Illumina) according to manufacturers instructions for RNA sequencing submission. The sequencing was performed with paired-ends (100 bp) using the Illumina Novaseq 6,000 Platform in the Center for Cell-Based Therapy Facility at Ribeirao Preto Medical School Ribeiro Preto.