title: GSM8689076: NIPC 4 hours post perfusion T2-rep 4; Rattus norvegicus; RNA-Seq
library_construction_protocol: Left ventricle tissue samples were homogenized in liquid nitrogen. Total RNA was then extracted from homogenized tissue samples of all experimental groups using TRIzol reagent® (Invitrogen Australia, Mt Waverely, Vic, Australia) and Direct-zolTM RNA miniprep kit (Zymo Research; Irvine CA, USA), NEBNext® Ribo-Zero Magnetic kit (rRNA removal) module (New England Biolabs, Ipswich, MA) was used to enrich mRNA from 1μg of total RNA. Barcoded libraries were generated using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (New England Biolabs) following the manufacturer's instructions. Deep sequencing was performed using Illumina NovaSeq PE 150 (San Diego, CA)