title: RNA-Seq of Equus Caballus: young female blood
design: Peripheral blood samples were collected from horses, total RNA extraction was performed using the Trizol method,  amplification of IGH from the IGHV gene segments and the constant region was performed by multiplex PCR. After library preparation and quantification, sequencing of the horses antibody heavy chains was performed. This procedure was carried out on the Illumina MiSeq 2x300 bp platform.