title: Shotgun metagenomics of mixed microbial community: digestate on day 5
design: For DNA extraction, microbial samples were processed using the DNeasy PowerSoil Kit (QIAGEN, Hilden, Germany), following the manufacturers protocol with an additional purification step using phenol:chloroform:isoamyl alcohol (IAA) to increase DNA purity. Total nucleic acid (RNA/DNA) was extracted using the RiboPure-Yeast Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA). The concentration and purity of nucleic acids were assessed before and after DNase treatment using a NanoDrop 2000 spectrophotometer and Qubit fluorometer (Thermo Fisher Scientific).