title: Site 14 subsoil sample 5:RNA-seq of soil
design: DNA was extracted from soil using the DNeasy PowerSoil Pro kit (QIAGEN); the V4 region of the 16S rRNA gene was amplified using the barcoded primer set 515F (GTGCCAGCMGCCGCGGTAA) and 806R (GGACTACHVGGGTWTCTAAT).