title: GSM8656374: Wild-type M. tuberculosis, stationary phase, replicate 4; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: Cultures were crash cooled with  frozen PBS and centrifuged at 4°C for 10 minutes at 4100 g. Cell pellets were resuspended in 1.2 ml Trizol and RNA extracted as manufacturer's instructions with the final RNA pellet being dissolved in 40 µl water. The RNA was DNase (Qiagen) treated for 10 minutes at 30°C followed by a second on-column DNase treatment using the Zymo RNA Clean and Concentrate columns and finally eluted in water Illumina TruSeq Paired-End kit as manufactures protocol