title: GSM8761092: Arabidopsis Wild type callus, biol rep1; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Samples were ground, and total RNA was extracted using the RNeasy Plant Mini Kit (Qiagen) following the manufacturer's instructions, including treatment with RNase-free DNase (Qiagen). Each RNA sample was loaded on agarose gel for integrity validation and quantified using NanoDrop. Sequencing was done at the Technion Genome Centre (TGC) according to Illumina's protocols. The TruSeq RNA V2 sample prep kit (RS-122) was used. Total RNA was polyA-selected, followed by fragmentation and random hexamer-primed reverse transcription. Indexed adapters were added, and cDNA was amplified by PCR for 15 cycles. Every four indexed libraries were loaded onto an individual lane of Illumina's HiSeq 2500 system.