title: GSM8656331: L. japonicus ASN symbiotic conditions, nodulated roots, biological replicate 2; Lotus japonicus; RNA-Seq
library_construction_protocol: Tissue was flash-freezed in liquid N2, ground with mortar and pestle and RNA extracted with a modified CTAB protocol Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP.