title: GSM8943899: E18.5 control cortex (Replicate 1); Mus musculus; RNA-Seq
library_construction_protocol: Mouse cortex samples were collected from E18.5 Emx1-Upf2cKO cortices and proceeded to total RNA extraction. All samples passed the QC test in the bioanalyzer analysis (RIN≥9.0). RNA-Seq libraries were constructed using Illumina TruSeq Stranded Total RNA LT Sample Prep kits (Illumina, RS-122-2101) according to the manufacturer's protocol. Briefly, 2 ug total RNA per sample was used for library construction. mRNA was purified and fragmented for first-strand cDNA synthesis. Then, second-strand cDNA synthesis was performed, followed by adenylation of the 3' ends of the double-stranded DNA fragments to facilitate adaptor ligation with unique index sequences. The libraries were conducted to confirm that the fragment sizes ranged from 200 to 650 bp. Indexed samples were pooled in equal amounts before sequencing. All premade RNA-seq libraries were sequenced using NovaSeq X plus platform by Novogene Corporation Inc., generating >120M reads per sample for subsequent analysis.