title: GSM8761351: Csa brain 12m 1_5; Mus musculus; RNA-Seq
library_construction_protocol: Brains were pulverized using mortal and pestle, and RNA was extracted using TRIzol-chloroform standard protocol. Quantification was performed using Qubit RNA High Sensitivity kit, and quality was analyzed using Bioanalyzer. Poly-A RNA were enriched using Poly(A)Purist Kit (ThermoFisher). mRNA libraries were prepared using SOLiD Total RNA-Seq Kit (ThermoFisher) according to manufacturer's instructions. Each sample was sequenced 5 times using AB SOLiD System (Thermo Fisher) in a 2x75bp format at the Core facility for scientific research in University of São Paulo (CEFAP-USP).