title: RNA-Seq of Chelymorpha alternans: larval foregut symbiotic organs
design: Larval foregut symbiotic organs were dissected and snap-frozen in liquid nitrogen. Total RNA was extracted using the QIAGEN RNeasy Mini Kit according to the protocol 4: Enzymatic Lysis and Proteinase K Digestion of Bacteria starting from step 7. RNA was quantified using the QubitTM RNA High Sensitivity (HS) kit. 40 ng of total RNA were used as input for RNA sequencing library preparation. Ribosomal RNA was depleted from total RNA using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat). RNA-Seq libraries were constructed using the NEBNext UltraTM Directional II RNA Library Prep kit.