title: scRNA-seq of HSEs: 3D_ED
design: Single-cell RNA sequencing libraries were generated using the Chromium Next GEM Single Cell 3 Kit v3.1 (10x Genomics) following the manufacturers protocol. Cells (NHEKs, NHDFs, and HUVECs) were enzymatically dissociated, and polyadenylated mRNA was captured and reverse-transcribed in GEMs. cDNA was amplified, fragmented, and prepared into sequencing libraries. Libraries were sequenced on an Illumina NovaSeq 6000 platform (paired-end 150 bp), with data aligned to the human reference genome (hg38) for downstream analysis.