title: GSM8942528: Input_rep2_H9TELC; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were crosslinked with 1% formaldehyde (Pierce cat. no. 28906) for 10 minutes at room temperature on a horizontal rocker and quenched with 2.5 M glycine. After nuclear lysis, chromatin was sheared in microtube-500 AFA (Covaris cat. no. 520185) using Covaris E220 Focused-Ultrasonicator with settings 5% duty, PiP 140, 15 minutes in 30 second bursts, cycles 200. Sequencing libraries were generated using 50% of the volume (8 uL)  from each ChIP using the KAPA Hyper Prep Kit (KAPA Biosystems, Wilmington, MA, USA) following manufacturer's instructions using 13 cycles of amplification. The quality of the library was assessed using High Sensitivity D1000 kit on a 4200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA).