title: GSM8513974: High DIPs, sc-RNAseq; Mus musculus; RNA-Seq
library_construction_protocol: The turbinate and adjacent mucosal tissues were isolated at 48 hpi. The tissues of three mice in each group were mixed together and digested with LiberaseTM (Roche) and DNase I (Roche) for 1 h at 37 ℃. After filtering through a 70-mm strainer, red blood cells were lysed using ACK buffer (Thermo Fisher). Cells were counted and prepared as single-cell suspensions for scRNA-seq. scRNA-seq library preparation was conducted using Chromium Next GEM Single Cell 3' Reagent Kit v3.1 according to the manufacturer's instructions for 10x Genomics. Briefly, mRNA from approximately 1.2×104 cells was reverse-transcribed into cDNA, and a unique barcode was added using poly(T) RT primers. After cDNA amplification, the scRNA-seq library was sequenced using the Illumina NovaSeq6000 sequencing platform.