title: GSM8761976: OXA_d16; Mus musculus; RNA-Seq
library_construction_protocol: ear+neck skin samples were pooled for n=3 mice per library and immediately digested using the Multi Tissue Dissociation Kit 1 (Miltenyi). To enhance the detection rate of fibroblasts, Collagenase Ia (1 mg/mL, Sigma) was used in place of Enzyme D. Digestion was carried out at 37°C for 1 hour with gentle agitation using the GentleMACS Dissociator Program H. The digested cell suspensions were passed sequentially through 100 μm and 35 μm cell strainers to remove undigested tissue fragments. To ensure a high cell viability, the suspension was further processed using the MojoSort™ Mouse Dead Cell Removal Kit (BioLegend) twice. After confirming a cell viability of ≥80%, the cells were resuspended in PBS and proceeded to library preparation according to the protocol provided by 10x Genomics (Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index)) Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index)