title: GSM9650700: MDA-MB-231 cells, TRIM27-KO, Olaparib, 24h, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from cultured cells using TRIzol reagent. RNA purity and concentration were routinely verified prior to subsequent library construction. Subsequently, 1 μg of high-quality total RNA was utilized to prepare mRNA sequencing libraries. Library sequencing and the following downstream bioinformatics analyses were performed by Frasergen Bioinformatics Co., Ltd. (Wuhan, China). mRNA was enriched from total RNA via oligo(dT) magnetic beads, followed by fragmentation and cDNA synthesis. The sequencing library was constructed through end repair, A-tailing, adapter ligation, and PCR amplification.