title: 16srRNA of ILFS soil:H45
design: The V3-V4 region of the 16S rDNA was amplified to access the abundance of prokaryotes (bacteria and archaea) from samples by using the updated primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3). The paired-end sequencing with 2 x 250 bp reads was performed in Illumina NovaSeq 6000 platform. Both amplification and sequencing were performed by Novogene Bioinformatics Technology using standard approaches as defined by the Earth Microbiome Project.