title: GSM9240330: Hoechst-CD133+ GL261 cells, PBS + cytokine cocktail treament, Day 6, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from GL261 cells was extracted using TRIzol™ reagent according to the manufacturer's protocol. The integrity of the extracted RNA was assessed by agarose gel electrophoresis. The RNA concentration was accurately determined using a NanoDrop spectrophotometer. A high-throughput paired-end RNA sequencing library was prepared following the standard Illumina protocol, and library quality was assessed with a Qubit Fluorometer and an Agilent Bioanalyzer.