title: Environmental DNA metabarcoding of Macao wetland (12S)
design: Environmental DNA was extracted from surface water samples collected from urban wetlands in Macao. DNA extracts were used as templates for PCR amplification targeting a ~170 bp fragment of the mitochondrial 12S rRNA gene using MiFish-U primers. Amplicons were purified and pooled for library preparation following BGIs standard amplicon sequencing workflow. Libraries were quantified with an Agilent 2100 Bioanalyzer, then sequenced on the DNBSEQ-G400 platform using paired-end 300 bp reads.