title: GSM8944339: rat retina, postnatal day 90, treated with hNPCs, rep 2; Rattus norvegicus; RNA-Seq
library_construction_protocol: Rats retinas were pooled, digested with papain, and dissociated into single-cell suspensions by manual trituration in ovomucoid solution. Single-cell suspensions from hNPC-treated and untreated retinas were labeled with DAPI. PKH26-labeled hNPCs, along with hNPC-treated and untreated retinal cells, were sorted using a FACS sorter and collected into 1.7 mL RNase-free tubes containing 2% BSA in PBS. Library preparation was performed using the 10x Chromium v3 single-cell reagent kit, following the manufacturer's protocol.