title: Branched-chain amino acid- and polar amino acid-amended culture 3
design: DNA was shipped to Molecular Research DNA Lab (Texas, USA) for sequencing using the updated Earth Microbiome Project (EMP) 16S rRNA primer set91,92: 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 806R (5-GGACTACNVGGGTWTCTAAT-3).