title: F114
design: The qualified DNA samples were randomly broken into 350 BP fragments by a Covaris crusher. The NEB Next UltraTM II DNA Library Prep Kit was used to build the Library, and the reagents and consumables recommended by the manual were strictly used. The whole library was prepared by the steps of end repair, ployA tail addition, sequencing linker addition, purification and PCR amplification. The constructed library was sequenced by ILLUMINATM