title: 16Smet metabarcoding of 1L Sterivex filtered deep seawater from Niskin Darya: deep seawater
design: 1L of deep seawater collected with Niskin Sedna was filtered through a 0.2 micron Sterivex. DNA was extracted using the Qiagen PowerSterivex kit. Primers targeting the 16S mitochondrial gene (Kelly et al. 2016) were used to generate amplicons sequenced on an Illumina MiSeq with v3 chemistry generating paired-end reads. Samples were dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B).