title: Thiolutin Is a Potential Fungicide for Controlling Phytophthora nicotianae and Its Mechanistic Study
design: This study aims to investigate the effect of thiolutin treatment on the gene expression profile of Phytophthora nicotianae, a pathogenic oomycete causing significant damage to agricultural crops. The experiment was designed to compare the transcriptomic changes between treated and untreated P. nicotianae samples. We cultured P. nicotianae isolates in liquid media under controlled conditions. The treatment group was exposed to a specific concentration of thiolutin for a defined period, while the control group was maintained under identical conditions without the treatment. Three biological replicates were prepared for both the treatment and control groups. RNA was extracted from the mycelial tissues, and RNA-Seq was performed using paired-end sequencing. The study seeks to identify differentially expressed genes in response to thiolutin treatment, which may provide insights into the molecular mechanisms underlying the antifungal activity of thiolutin and potential targets for disease control strategies.