title: RNA-seq of C. elegans: E. faecalis V583 volatile exposure
design: C. elegans and bacterial strains were cultured separately according to standard growth protocols on  6cm plates until worms reached the L4 larval stage. C. elegans were fed E. coli strain OP50 and cultured at 20C throughout the experiment. To begin volatile exposure, the C. elegans and bacteria-containing plates were placed together without lids in a 235ml sealed plastic container for 24 hours until young adulthood, collected in M9 and rinsed 3x with M9. Animals were then flash-frozen in TRIzol (Invitrogen) and freeze/thawed 3x in liquid nitrogen. RNA was purified using a RNeasy Mini Kit (Qiagen). RNASeq library preparation (4 biological replications per treatment) was performed using Roche products, KAPA Biosystems mRNA HyperPrep Kit and KAPA Unique Dual Index Adapters. Thermo Fisher Scientific NanoDrop and Qubit instruments were used to measure nucleic acid concentrations.  Agilent BioAnalyzer was used to determine the quality of pre-library Total RNA and RNASeq libraries.