title: Figured shrub-like, 09/06/2023, fraction 1
design: To extract total RNA, plant samples were homogenized in CTAB lysis buffer (pH 4.85.0) and mercaptoethanol was added to a final concentration of 2%. Then a mixture of chloroform-isoamyl alcohol (24:1) was added. RNA was precipitated with 25 mM LiCl and then purified again with SDS buffer (1 M NaCl, 0.5% SDS, 10 mMTris-HCl (pH 8.0), 1 mM EDTA). RNA was reprecipitated with absolute isopropanol