title: direct RNA ONT sequencing of P. falciparum UMB-MWI Trophozoites in vitro polyadenylated
design: Libraries were prepared from 150 ng RNA, which was in vitro polyadenylated E. coli Poly(A) Polymerase (New England Biolabs) for 75 sec at 37C, using the SQK-RNA002 kit and sequenced on a MinION flow cell (R9.4.1). Only reads above 200 nt were sequenced. Raw fast5 reads generated with guppy-4.2.2.