title: GSM8605855: col, Submergence , rep3(bak1-3 bkk1-1); Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Germinating seeds or six-day-old seedlings with or without submergence treatment were harvested and ground into a powder in liquid nitrogen. Total RNA was extracted using a Plant RNA Kit (Omega, R6827). Messenger RNA was purified from total RNA using poly-T oligo attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. After library quality control, quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.