title: metabarcoding of seawater eDNA: MiFish-U targeting 12S for Actinopterygii
design: eDNA extracted with QIAGEN Dneasy Blood and Tissue kit, PCR performed with KAPA HiFi and MiFish-U primers, size-selection with MagBead purifier, 2nd PCR library preparation with Illumina TruSeq indices, sequencing on MiSeq