title: GSM8656502: Arabidopsis seedlings, ATAC, sav3-2, 7-day-old, LRFR 25h, R1; Arabidopsis thaliana; ATAC-seq
library_construction_protocol: Nuclei were isolated using the INTACT method (You et al., 2017). Briefly, nuclei were conjugated to Dynabeads™ M-280 Streptavidin and captured on the magnetic rack. Supernatant was removed and beads were washed twice with NPB, then left resuspended in NPB on ice. The purified nuclei (25000 – 50000 nuclei) were incubated with Tagment DNA TDE1 Enzyme and Buffer mix at 37°C for 30 min. DNA was purified with Wizard SV Gel and PCR clean-up system. Library was amplified using 2X KAPA HiFi HotStart and Nextera Primer Mix (i7+i5). Amplified libraries were purified with ProNEx Size selective Purification System and only libraries with fragments between 100 bp and 600 bp, peaking at 300 bp were considered.