title: GSM9391895: Mono_Young_1; Mus musculus; RNA-Seq
library_construction_protocol: Purification of RNA was performed with RNeasy Mini Columns (QIAGEN, Valencia, CA) with RNase-free DNase solution (QIAGEN) applied to degrade contaminating genomic DNA. RNA quantity was assessed with Nanodrop spectrophotometry (ThermoFisher). cDNA libraries were prepared using 100 ng of total RNA according to the manufacturer's instructions for the Illumina Stranded mRNA Prep, Ligation (Illumina, San Diego, CA). The concentration and size distribution of the completed libraries were determined with the Agilent TapeStation's D1000 ScreenTape and Qubit fluorometry.