title: GSM8862713: WT3_D2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested at 24, 48hrs of activation of CD4+ T-cells. Total RNA was extracted using RNeasy Plus Micro kit (Qiagen). 1.5ug of RNA was used for construction of sequencing libraries. 100ng of the RNA was used to perform Agilent TapeStation QC analysis to verify RNA integrity and generate RNA Integrity number (RIN). Using Nova-Seq 6000 S1 200 cycle reagent kit, 25 million pair-ended reads were recorded for construction of mRNA libraries.