title: GSM9262934: RNAseq_RPM_tumor_ent_1; Mus musculus; RNA-Seq
library_construction_protocol: Flash-frozen tumor tissue was manually dissected with a scalpel blade and then fully homogenized using a TissueLyzer. After centrifugation, supernatant from the tissue lysates was transferred to an AllPrep DNA spin column (Qiagen) for DNA extraction. Flow-through from the column was transferred to a RNeasy spin column (Qiagen) for RNA purification. Using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775) and  NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490)