title: GSM9262945: Spatial-Seq, Synovium, hip OA, OA001; Homo sapiens; OTHER
library_construction_protocol: Human FAI (n = 4) and hip OA (n = 6) synovial tissues were isolated and placed in collection medium (DMEM/F-12, GlutaMAX™ supplement, #10-565-018, Gibco™, with 1% Penicillin-Streptomycin (10,000 U/mL), #15140122, Gibco™, and 10% Fetal Bovine Serum, #F0926-100, Sigma-Aldrich). Harvested synovial tissues were then transferred to 10% neutral buffered formalin solution. Upon 24-hour fixation at room temperature and paraffin embedding, FAI and hip OA synovial samples were sectioned at 8 m in an RNase-free environment (#7003 ThermoFischer) and placed on the individual spatially barcoded capture areas of a 10X Genomics Visium Gene Expression Slide (10X Genomics, Pleasanton, CA). Following tissue placement, slide drying, and overnight incubation in a desiccator, the slide was deparaffinized, H&E stained, and imaged using a VS120 Slide Scanner (Olympic) at 20X magnification. Sequence ready libraries were then constructed according to the manufacturer's instructions (10X Genomics, CG000407). Final libraries were sequenced on the NovaSeq 6000 sequencer (Illumina, San Diego, CA) to obtain at least 50,000 reads per spot.