title: GSM8944065: cancer cells with C2 CAF; Homo sapiens; RNA-Seq
library_construction_protocol: Co-cultured cells were harvested and made a single cell suspension by incubating with accutase in 37°C for 3-4 minutes followed by filtering through a 70 μm cell strainer. After a brief centrifugation at 300g for 3 minutes, cell pellets were resuspended in the cultured low serum media. Using trypan blue, the cells were counted and checked for viability. The suspension having more than 80% cell viability was diluted to a concentration of approximately 1000 cells/µL. Chromium controller (10X Genomics), chromium 3ʹ library, Gel bead kit and finally the chromium Single cell 3ʹ chip kit (10X Genomics) were used according to the manufacturer's instruction to make Single cell gel bead-in emulsion and for sequencing library construction.