title: GSM8968743: Immune Cells from CT2A bearing wildtype mouse; Mus musculus; RNA-Seq
library_construction_protocol: CT-2A tumor cells were implanted intracranially, and the resulting tumor was removed after 14 days and digested into single-cell suspension using the adult brain dissociation kit (Miltenyi) according to the manufacturers instructions. CD45+ cells were isolated via magnetic bead isolation (Miltenyi) and submitted to the Northwestern University NUseq facility core for single cell library preparation and sequencing. AOPI fluorescent staining was used to determine cell number and viability Nexcelom Cellometer Auto2000 with. Sixteen thousand cells were loaded into the Chromium Controller (10X Genomics, PN-120223) on a Chromium Next GEM Chip K (10X Genomics, PN-1000127), and processed to generate single cell gel beads in the emulsion (GEM) according to the manufacturer's protocol. The cDNA and library were generated using the Chromium Next GEM Single Cell 5' Reagent Kits v2 (10X Genomics, PN-1000283) according to the manufacturer's manual.