title: GSM8577698: ShamOpe; Mus musculus; RNA-Seq
library_construction_protocol: Tissue frozen with liquid nitrogen was placed between aluminum foil on dry ice and crashed with a hammer. Crushed tissue was placed in Lysis Buffer to extract nuclei and debris was removed using Debris Removal Solution. DAPI was added to the resulting nuclear suspension and DAPI-positive nuclei were sorted and collected using a BD FACS Aria III instrument. Collected nuclei were washed with PBS containing 1% Bovine Serum Albumin (BSA) and resuspended at the appropriate concentration. Libraries were prepared using the Chromium Next GEM Single Cell 3' Kit v3.1(10x Genomics) according to the manufacturer's protocol.