title: 1835_CA_Fungus_Pilot_SN-36_Slant_2
design: Extracted DNA was quantified using the Quant-iT dsDNA Assay Kits (Thermo Fisher Scientific, Carlsbad, CA, USA). Fluorescence was measured on a FLUOstar Omega (BMG LabTech, Ortenberg, Germany). Sequencing libraries were prepared using a custom modified version of the DNA prep kit (Illumina, San Diego, CA, USA) based on the Hackflex protocol (1) using 20 ng input DNA. The quality of the sequencing libraries was assessed by measuring concentration on a Qubit 3.0 fluorometer using the Qubit dsDNA quantification assay kit (Thermo Fisher Scientific) and by performing a fragment analysis using the TapeStation 4200 with the D5000 Screen Tape assay kit (Agilent, Santa Clara, CA, USA). Quality checked final libraries were multiplexed at equimolar ratios and sequenced on a NovaSeq 6000 (Illumina) using a 200-cycle S4 reagent kit generating 2x100 bp paired-end reads. As described in PMID: 35014949.