title: Multiome_p4_pituitary
design: Sn multiome was performed following the Chromium Single Cell Multiome ATAC and Gene Expression Reagent Kits V1 User Guide (10x Genomics, Pleasanton, CA). Nuclei were counted using propidium iodide fluorescence (Revvity Cellometer counter), transposition was performed in 10 ul at 37 C for 60 min, targeting 500-20,000 nuclei, before loading of the Chromium Chip J (PN-2000264) for GEM generation and barcoding. Following post-GEM cleanup, libraries were pre-amplified by PCR, after which the sample was split into three parts: one part for generating the snRNA-seq library, one part for the snATACseq library, and the rest was kept at -20C. SnATAC and snRNA libraries were indexed for multiplexing (Chromium i7 Sample Index N, Set A kit PN-3000262, and Chromium i7 Sample Index TT, Set A kit PN-3000431 respectively).