title: GSM8689443: mRNA-Seq for EZH2 splicing reporter CRASP-Seq assay in HEPG2 cells; Homo sapiens; OTHER
library_construction_protocol: Total RNA was extracted using RNeasy plus miniprep RNA isolation kit and polyA mRNAs were enriched using oligo-dT magentic beads cDNA synthesis was performed using primers with Unique Molecular Identifier (UMI) sequences.  cDNAs were PCR amplified using illumina sequencing indexes such as i7,P7,i5, and P5 for paired-end sequencing. Next-generation sequencing was performed on an Illumina NovaSeq 6000 platform (200 cycles kit) except for LMNA Base Editor screens which were performed on an Illumina NovaSeq X Plus platform.