title: GSM8761831: H05shabroLA2167sclero; Solanum habrochaites; RNA-Seq
library_construction_protocol: RNA was isolated using the Quick-RNA Plant Kit (ZYMO RESEARCH). We pooled 2x2cm parts of two independent leaves into DNA/RNA-shield (ZYMO RESEARCH). 0.5ng/µL RNA was used for library preparation. We used the 3' mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices (12nt) with PCR Add-on and Reamplification Kit V2 for Illumina, and the SIRV-Set 3 for in-house library preparation, following the manufacturers iinstructions.