title: V3-V4 16S rRNA of bat gut metagenome: Nyctalus noctula
design: DNA from fecal samples was extracted with the SKYAmp Stool DNA Kit (SkyGen, Moscow, Russia). V3-V4 16S rRNA library preparation was conducted with the Quick- 16S NGS Library Prep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturers protocols. Primers used for the amplification of V3-V4 regions of the 16S rRNA gene were 341F (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG) and 806R (GACTACNVGGGTMTCTAATCC). The final library was sequenced on Illumina MiSeq with a v3 Reagent Kit (600 cycles) according to the manufacturers instructions (Illumina, San Diego, CA, USA).