title: skin microbiome of captive Rhinella marina at Middle Point, NT, Australia
design: Captive toads (brought into captivity from the surrounding wild population) held in semi-natural outdoor enclosers were sampled to detect temporal patterns in skin bacterial microbiomes and compare with wild toads. Toad was rinsed with milli-q water before entire body was swabbed with Copan flocked swab avoiding the face and cloaca. Sample was stored in 300uL Zymo DNA/RNA Shield and refrigerated until DNA extraction with Norgen's Microbiome kit. Amplicons of the bacterial V4 region of 16S rRNA gene were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.