title: Nanopore sequencing of isolated bacterium
design: Genomic DNA was extracted from fresh patina collected from a Zobell Marine Agar culture (ZMA) culture grown at 30C for 3-5 days. The patina was dissolved in the DNeasy Blood & Tissue Kit (QIAGEN) lysis buffer and processed as described by the manufacturer. The extracted DNA was assessed for quality by electrophoresis on 1.2% agarose gels and quantified using a Qubit 3.0 Fluorometer, Qubit dsDNA BR assay kit (Thermo-Fisher Scientific, Waltham, United States). The two genomes were sequenced using an Oxford Nanopore Technology GridION (Pacific Biosciences) at the Bioscience Core Lab of KAUST.