title: GSM8656516: Cyclosporin A-treated sample; Mus musculus; RNA-Seq
library_construction_protocol: Nuclear dissociation was performed using the Nuclei EZ Prep Kit. A sliced, snap-frozen kidney was minced and homogenized in ice-cold EZ Lysis Buffer. After incubation on ice, the homogenate was filtered through a 40-μm strainer and centrifuged. The pellet was resuspended with EZ Lysis Buffer, centrifuged, resuspended in Dulbecco's phosphate buffered saline with 1% fetal bovine serum and filtered through a 5-μm strainer. Formation of gel beads in emulsion, barcoding, cDNA amplification and library construction were performed according to the manufacturer's instructions for Chromium Next GEM Single Cell 3ʹ Kit v3.1 (10x Genomics).