title: GSM9241214: No6; Mus musculus; OTHER
library_construction_protocol: At 6 days post-injection, the mice were sacrificed 30 minutes after the final procedure. Immediately after euthanasia, thoracic tissues were collected from the left lung parenchyma adjacent to the chest wall. The samples were fixed in 4% paraformaldehyde for 24 hours. For spatial transcriptomic analysis, tissues were sectioned at 5 μm thickness. Visium Mouse Transcriptome Probe Set and Visium V4 Slide - Probe-based v2 chemistry were used to extract total RNA and hybridize transcriptomic probes to the Visium slide. Sequencing library preparation were performed according to standard protocol of the Visium CytAssist Spatial Gene Expression Reagent Kits User Guide.