title: Tk rxn, H2O treated 65-3h
design: Synthetic constructs were in vitro transcribed and treated with TkNat10 and cofactors at the specified time and temperature, then subjected to ac4C-seq using either NaCNBH3 or a vehicle (ddH2O) control. RNA was reverse transcribed, amplified, barcoded, and analyzed by Illumina sequencing.