title: GSM8761164: TBI026_2VST_CD4; Homo sapiens; RNA-Seq
library_construction_protocol: PBMC were separated by Ficoll-Hypaque gradient centrifugation and stored as viable PBMC in liquid nitrogen.Later PBMC were thawed, stained with anti-CD3, CD4, CD14, CD16, CD20, and CD56 antibodies and cells were sorted as CD4 and monocytes.otal RNA was extracted from sorted CD4 T cells and monocytes using the Quick RNA Microprep kit (Zymo research CA, USA) according to the manufacturer's instructions. RNA concentration and quality were assessed using a Qubit fluorometer (Life Technologies, USA) and TapeStation 2200 system (Agilent Technologies, USA). Libraries were prepared using the Ovation Solo RNA sequence system (NuGen Technology, CA USA) following manufactures protocol and RNA sequencing was performed using the Illumina MiSeq 2x150 bp paired-end sequencing platform.