title: GSM8463249: hASCs cells, Passed_1; Homo sapiens; RNA-Seq
library_construction_protocol: After the neutralization and removal of trypsin, the cell pellet was resuspended in 𝛼-MEM and subdivided into aliquots containing 0.5 × 106 cells. Cells were passed through the microfluidic chip and collected at the outlet. After squeezing, hASCs were left for 5 min at room temperature. Then, RNA was extracted from the cells using an RNeasy Mini Kit (Qiagen) and quantified using a NanoDrop spectrophotometer. Next, the mRNA samples were converted to cDNA using the High-Capacity cDNA Conversion Kit (Applied Biosystems,USA). RNA libraries for Rna-seq were prepared using SMARTER RNA-Seq Library Prep Kit following manufacturer's protocols