title: Iso-Seq of Arctopsyche grandis: silk glands
design: RNA was extracted from seven silk gland and two thoracic muscle samples of Arctopsyche grandis using a Trizol method, and the RNA quality was assessed with a bioanalyzer. Iso-Seq libraries were constructed with bc04 to bc12 barcodes. Barcoded cDNAs were pooled and processed with the Kinnex method. Long-read sequencing was performed on a PacBio Revio at the DNA Sequencing Center at Brigham Young University (Utah, USA).