title: GSM8761694: Regular RNA-seq profile of N2 C. elegans at 64 hours post L1 seeding; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Animals were transferred to 96-well plates for high-throughput RNA extraction following protocol described in the WPS method paper. Importantly, this method only extracts total RNA from adult animal tissues and leaves any eggs intact. Extracted total RNA was barcoded during reverse transcription to enable early pooling of samples. Multiplexed RNA-seq libraries each containing ~50 samples were constructed following a protocol adapted from the CEL-seq2 single-cell RNA-seq library construction method (Hashimshony, Genome Biology, 2016). For detailed protocols, refer to our WPS method paper. This library construction method produces a multiplexed RNA-seq library comprising ~50 samples. The sample pooling strategy differs from standard WPS due to historical reasons. Specifically, samples from the main plate were pooled by replicates, i.e., samples in the same biological replicate were pooled together. Samples from the supplementary plates were pooled in a mixed manner: the supplementary plate - lib1 contains samples from up to three replicates; the supplementary plate - lib2 contains samples only from replicate #1, except for vector control samples. For regular RNA-seq (WT_64hph_regular_RNA_seq), library construction was performed using BGI Transcriptome Resequencing Pure-sequencing service.