title: RNA-seq of native heart valves: alcified aortic valve disease
design: Heart valve fragments were homogenized in TRIzol, and total RNA was extracted with DNase treatment, followed by quality and quantity assessment using Agilent Bioanalyzer 2100, NanoDrop 2000, and Qubit 4 Fluorometer. Poly(A)+ mRNA was isolated, converted into barcoded libraries, quality-checked, quantified by qPCR, and sequenced on the MGIseq-2000.