title: EDTA iron fertilizer treatment group citrus rhizosphere soil sample 4 (CRX1824888; CRA027379)
library_construction_protocol: Soil genomic DNA was extracted using a Soil Genome DNA Extraction Kit according to the manufacturer's instructions. The V3-V4 region of the bacterial 16S rRNA gene was amplified using 2xPhantaMax Master Mix and primers 341F and 806R. PCR reaction contained 30 ng of template and fusion PCR primers. The PCR program was as follows: 95 deg C for 3 min; 30 cycles of 95 deg C for 15 s, 56 deg C for 15 s, and 72 deg C for 45 s; final extension at 72 deg C for 5 min. Amplicons were purified using DNA magnetic beads, circularized to generate single-stranded DNA, and amplified via phi29-mediated rolling circle replication to produce DNBs. Sequencing was performed on the MGISEQ-2000 platform using paired-end 300 bp cPAS chemistry. submitted_instrument_model: MGISEQ-2000