title: GSM9262914: si-NS+AngII; Mus musculus; ATAC-seq
library_construction_protocol: Add 1M DNase, mix thoroughly, and incubate at 37℃ for 30 minutes. Then, add 1 ml of pre-cooled EPITM ATAC Lysis Buffer and mix again. DNA were resuspended in 25 µL of 2×HiFi PCR mix, 1 µL of each Nextera i5 primer（N5xx） and Nextera i7 primer（N7xx）.The following PCR program was performed: 72 °C for 5 min, 98 °C for 1 min, 14 cycles at 98 °C for 15 s, 60 °C for 30 s, and 72 °C for 1 min.