title: GSM8969382: TN cerebellum; Mus musculus; OTHER
library_construction_protocol: 60 min after fear conditioning or retrieval test, mice were anesthetized with isoflurane and perfused with normal saline. Extracted brains were flash frozen in isopropyl alcohol at -70 °C. Frozen brains were embedded in optimal cutting temperature (OCT) compound and were stored at -80 °C until sectioning. Visium libraries were constructed following the manufacturer's instructions. Sequencing was performed on a Novaseq 6000 platform using S1 sequencing reagents (Illumina, Cat No. 20028318), targeting 120 million reads per library using dual indexing.