title: GSM9240923: MOLM-13, PTBP1, biol rep 2 (CUT&Run); Homo sapiens; OTHER
library_construction_protocol: DNA was extracted using the phenol–chloroform extraction method and precipitated using chilled 75% ethanol. DNA pellets were dissolved in 30 µl of 0.1 × TE (1 mM Tris pH 8, 0.1 mM EDTA) supplemented with a 1:400 dilution of 10 mg/ml RNase A (Thermo Scientific cat. no. EN0531) and incubated in a 37 °C water bath for 15 min. Libraries were amplified by addition of 2 µl each of barcoded 10 mM i5 and i7 primer solutions and NEBNext HiFi 2 × PCR Master mix (NEB cat. no. M0541) with 13 rounds of amplification as described previously. Sequencing libraries were purified using a 1.3× ratio of HighPrep PCR Cleanup beads (MagBio genomics cat. no. AC-60500) as per manufacturer's instructions and eluted in 0.1 × TE. Library quality and concentrations were determined using the D1000 TapeStation system (Agilent).