title: GSM8831016: Vehicle RELA Naive RNA replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Total cellular RNA was extracted using the GenCatch Total RNA Mini Prep Kit (Epoch Life Science, Missouri City, TX, USA) following the manufacturer's protocol. The extracted RNA was assessed for quality and quantity using a NanoDrop spectrophotometer (Thermo Fisher Scientific) and Qubit Fluorometer (Invitrogen). RNA integrity was verified using an Agilent Bioanalyzer or TapeStation. 1 μg of total RNA was used for downstream applications, including library preparation. RNA libraries for RNA sequencing were prepared using Novogene and following the Illumina manufacturer's protocol.