title: PAW100
design: For RNA sequencing, library preparation was initiated by assessing the quality and quantity of the extracted RNA using Bioanalyzer 2100. High-quality RNA was used to construct sequencing libraries. This process involves RNA fragmentation, synthesis of the first and second cDNA strands, end repair, A-tailing, adapter ligation, and enrichment of the library through PCR. The prepared libraries were quantified and validated for quality before sequencing on an Illumina high-throughput sequencing platform.