title: RNASeq of control and ME2 kd cells
design: Stranded RNA-seq libraries were constructed using NEBNext poly(A) mRNA isolation kit followed directly by IDTs XGen Broad Range RNA Library Prep Kit and the established protocols.  The library construction was done using up to 1 g of RNA. Each of the libraries was indexed during library construction in order to multiplex for sequencing. Libraries were quantified using Invitrogens Qubit 4 fluorometer and checked for size and quality on Agilents 2100 Bioanalyzer.  Samples were normalized and pooled onto a 150 paired end run on Illuminas NextSeq 2000 Platform to obtain 20M reads per sample.