title: WGS of soil metagenome (Winter, Ridge, 3)
design: Samples were sieved (need to check the mesh mm) to remove large debris (e.g., roots, twigs), homogenized using aseptically clean blender, and 0.25-0.28 gr were used for DNA extraction using the Qiagen DNAeasy PowerSoil extraction kit. Next, DNA extracts were processed at the UCI Genomics Research and Technology Hub (https://research.uci.edu/shared-facility/genomics-research-and-technology-hub/). DNA extract concentrations were normalized and metagenomic DNA libraries were created using Illumina DNA Prep kit and indexed with unique sets of dual barcodes. Finally, Paired-End sequencing (150 cycles) was performed on an Illumina NovaSeq 6000.