title: GSM9240278: MIII.0; Mus musculus; RNA-Seq
library_construction_protocol: RNA extracted with Zymo quick-RNA MicroPrep kit according to the manufactuer's instructions Library preparation was performed starting with 5 ng of total RNA using the SMART-Seq Stranded Kit (Cat. #634444; Takara Bio) together with the SMARTer RNA Unique Dual Index Kit (Cat. #634452; Takara Bio) to generate ribosome-depleted libraries. Library quality was assessed on a Fragment Analyzer (Advanced Analytical, Ames, IA, USA) with the High Sensitivity NGS Fragment Analysis Kit (Cat. #DNF-474), confirming excellent quality (mean concentration 121 ± 41 nmol/L; mean library size 456 ± 29 bp). Samples were pooled to equal molarity and sequenced in paired-end 51-base mode (plus 8 bp index 1 and 8 bp index 2) on an Illumina NovaSeq 6000 using an SP flow cell.