title: Whole-metagenome sequencing of sludge: P.LL WWTP, Week 1
design: 250 mg of sludge was weighed using a precision scale (Mettler Toledo, MS-S/MS-L model), transferred into sterile Eppendorf tubes, and stored at 20C until DNA extraction. Extraction of DNA from sludge samples was performed using the DNeasy PowerSoil kit (Qiagen Laboratories, Inc.) according to the manufacturer's instructions. The concentration and the quality of the DNA was tested using a NanoDrop Spectrophotometer 8000 (Thermofisher Scientific, Inc), and all of the DNA extracts were stored at 20C until DNA library preparation. DNA samples were sent to Novogene UK for DNA library preparation with the NEBNext Ultra IIDNA Library Prep Kit (Cat No. E7645; New England Biolabs), and for paired-end 150 bp sequencing on a single lane on a NovaSeq X Plus Series platform (Illumina) using the 25B flow cell.