title: GSM8690775: Decr2 knock down; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were isolated from mice. For TIL isolation, tumors were digested using an enzyme mix containing Collagenase (1mg/mL, Sigma-Aldrich: C5138), DNAse (200 µg/mL, Sigma-Aldrich: D5025), and Hyaluronidase (100 μg/mL, Sigma-Aldrich: H6254) for 30 min at 37oC while rotating. The tumor suspension was filtered through a 50 μm filter and washed with PBS. Tumour and immune cells were pre-enriched using density gradient centrifugation (Ficoll, Sigma-Aldrich). The cell pellet was stained with live/death staining dyev and anti-CD45 antibodies. The CD45+ live cells were sorted by FACs big foot. Library was performed according to manufacter's instructions (single cell 3' v2 protocol, 10X Genomics)