title: GSM8862382: HET_Female_H3K79me2_R1; Mus musculus; ChIP-Seq
library_construction_protocol: Cortical tissue was homogenized with a douncer in homogenization buffer supplemented with 1 mM DTT, 0.15 mM spermine, 0.5 mM spermidine, RNasin ® Plus Ribonuclease Inhibitor (Promega N2611), and EDTA-free protease inhibitor (Roche). Nuclei were isolated using an iodixanol density gradient and ultradentrifugation. TruSeq ChIP Library Preparation Kit