title: GSM8577601: B1_HLA-A2_CAR_unstim; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Qiagen RNeasy Micro Kit and RNA was eluted in 14 µL RNAse-free water. RNA quality was assessed using the Tapestation system 4200 and High-Sensitivity RNA screentape (Agilent). 7 µl of the RNA was used for generating RNA-seq libraries using the SMART-seq Stranded Kit from Takara. Indexed libraries were pooled in an equimolar ratio and sequenced on an Illumina NextSeq 550 machine with NextSeq 500/550 High Output Kit v2.5 (75 cycles) or NextSeq2000