title: GSM8514049: PDX tumor, NBI-961, replicate 3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated from the TM00098 PDX tumors according to the manufacturer's protocol (RNeasy Plus Mini Kit, Qiagen, Hilden, Germany). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.