title: GSM8762122: Mus musculus Macrophage CD11cCre Casp8flox/flox Female Old, Replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Brain tissues were harvested immediately following perfusion with ice-cold HBSS. Brains were dissociated using a combination of mechanical and enzymatic digestion (Liberase TL and DNase I), and cell populations were sorted by FACS based on surface markers. RNA was extracted from FACS-purified populations using the PicoPure RNA Isolation Kit following the manufacturer's protocol. RNA-seq libraries were prepared using the QuantSeq 3′ mRNA-Seq Library Prep Kit for Illumina (Lexogen Inc.). Libraries were indexed and amplified according to the manufacturer's instructions. Samples were excluded based on experimental and technical factors that impact their quality.