title: GSM8970260: Curioseeker Spatial Transcriptomics, vascularized human lung organoids (vHLuO); Homo sapiens; OTHER
library_construction_protocol: 5 scrolls of tissue sections of 10um thickness were collected to derive an RNA integrity number (RIN) to test the tissue's RNA quality. All organoids' RIN numbers were higher than 8 to pass the quality test. One 10um thickness tissue section was placed on the Curio Seeker tile and mounted with 30um section of CryoCube. Then, the section was processed for hybridization, reverse transcription, and tissue clearance to yield Curio Seeker Bead resuspension, according to the manufacture's instruction. 11 cycles of PCR was utilized for second-string synthesis and cDNA amplification. The purified cDNA was applied for Tagmentation with Dual Indexing Primer V2 and consequent library cleanup and QC