title: GSM8943677: DMSO 4sUDRB-seq 0 min rep2; Homo sapiens; OTHER
library_construction_protocol: Total RNA was extracted using TRNzol according to the manufacturer's instructions. The total RNA was fragmented by treatment with NaOH at a final concentration of 0.2 M for 15 minutes, followed by neutralization with Tris-HCl (pH 6.8) at a final concentration of 0.5 M. Fragmented RNA was purified by isopropanol precipitation. After biotinylation of 4sU-labeled RNA, purification of biotinylated RNA with Dynabeads M-280 streptavidin and rRNA depletion, strand-specific RNA-seq libraries were constructed as previously described (Zhong et al., 2011). Libraries were sequenced on Illumina NovaSeq X Plus.