title: metabarcoding of seawater eDNA: MiFish-U targeting 12S for Actinopterygii
design: eDNA extracted with QIAGEN Dneasy Blood and Tissue kit, PCR performed with MiFish-U primers, size-selection with GeneRead Size Selection kit, 2nd PCR library preparation with Illumina TruSeq indices, sequencing on MiSeq with v2 Nano 300 kit