title: GSM8656997: 10X lane 3; Homo sapiens; RNA-Seq
library_construction_protocol: Breast tissues were chopped into a slurry, then digested overnight in collagenase type III and hyaluronidase in a spinner flask at 37°C. Filtration through 100 µm cell strainer was used to collect epithelium-enriched tissue microfragments, which were then cryopreserved. For sequencing experiment, the tissue microfragments were thawed and digested to single cells by trypsin treatment, followed by DNAseI and dispase. Samples were then labeled by MULTIseq LMO barcodes, and then pooled and sorted for live single cells as DAPI-negative cells. The single live cells were encapsulated into droplets on the 10X Chromium Controller, and library prep for sequencing was performed according to the 10X Chromium Single Cell 3' v3.1 User Guide Rev D and MULTI-seq library preparation protocol (McGinnis et al. 2019).