title: CRISPR-screening of Homo sapiens, ctrl
design: A CRISPR screeningbased experimental strategy was employed. HUES8 cells were transduced with a metabolism-related sgRNA library delivered by lentivirus, followed by differentiation toward definitive endoderm. Cell populations representing the top 30% and bottom 30% of CXCR6-positive fractions were isolated and subjected to sequencing.