title: GSM8690331: Caco-2, DFC, Deoxygenated medium, 13DPS, Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed with ice-cold lysis buffer (4 M GITC, 0.02 mM Tris-HCl, 10 mM NaAcetate, 25 mM EDTA, 0.1% Triton X-100, 2 mM DTT) and RNA was extracted by a phenol-chloroform extraction. Ribosomal RNA was depleted uisng NEBNext rRNA Depletion Kit (Human/Mouse/Rat). RNA libraries were then prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit.