title: GSM8635074: balixafortide/anti-PD-1 tumor 3; Mus musculus; RNA-Seq
library_construction_protocol: Tumors removed from mice; digested with collagenase/DNAse I in Miltenyi OctoDissociator; following lysis of red blood cells, isolation of CD45+ cells with immunomagnetic beads; recovered cells used at a ratio of 3:2 CD45+ to CD45- cells for DropSeq single cell RNA sequencing bar coded single cells with polyA enrichment for RNA for sequencing