title: GSM8634399: Base editing efficiency of circular gRNA with AC5 linker at 9 gene sites with Cas12f-ABE(C-AC5 group), repeat3; Homo sapiens; OTHER
library_construction_protocol: The HEK293T transfected cells were washed with 1x PBS, and harvested by addition of lysis buffer with proteinase K, 55℃ overnight. And then the genomic DNA was extracted with the steps of ethanol precipitation. The targeted genome regions of interest were amplified with the first round PCR by Gold Medal Mix to add unique indexes for each sample. Each sample was then amplified by the second round PCR using P5 and P7 primers. All PCR products were mixed into one pot and purified by DNA Clean Beads.