title: GSM9262844: SM8_NFIX_HCcontrol_2x; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from sorted cells using RNeasyMicro Kit (Cat.No. 4004, QIAGEN). RNA integrity (RINR8.0) was assessed using the High Sensitivity RNA Analysis Kit (DNF-472-0500, Agilent) on a 12-Capillary Fragment Analyzer. cDNA synthesis and Illumina sequencing libraries with 8-bp single indices were constructed from 10 ng total RNA using the Revelo RNA-seq High Sensitivity kit (Cat.No. 30184149, TECAN).