title: scRNAseq of in vitro coculture of neurons and astrocytes
design: Tri cocultures of neurons and astrocytes were performed . Then, cells were counted and processed for library preparation according to manufacturers instruction (10X Genomics). To avoid the batch effect all samples were prepared and sequenced at the same time. Cells were loaded onto a Chromium Next GEM Chip J with reverse transcriptase master mix, gel beads and partitioning oil. Gel beads in emulsion (GEMs) were generated and cDNA was purified with DynaBeads and library quantification was performed. Sequencing was performed on a NovaSeq (Illumina).