title: ITS DNA sequecning of the metagenome of the phyllosphere and thatch of turfgrass treated with different levels of nitrogen fertilizer
design: The sequencing library for fungal communities was prepared by amplifying the ITS2 region of the rRNA gene using the 5.8S-Fun/ITS4-Fun primers. PCR was performed with the KAPA Biosystems PCR kit, and products were cleaned using the ZR-96 Zymoclean Gel DNA Recovery kit. The amplicons were normalized with the Mag-Bind EquiPure Library Normalization kit, pooled in equal volumes, and quantified using a Qubit 3.0 Fluorometer. The final library was diluted to 4 nM before sequencing on the Illumina MiSeq platform using a 2  300 bp paired-end approach.