title: WGS of human intestine organoid with GH
design: Genomic DNA samples were assessed for concentration using a Qubit fluorometer (ThermoFisher Scientific, Waltham, MA) and for quality using the 4200 TapeStation (Agilent Technologies, Santa Clara, CA).  Five hundred ng of gDNA was used for library construction using the Illumina DNA Prep library preparation kit (Illumina, San Diego, CA) following the manufacturers user guide.  Five cycles of PCR were performed.  Library concentration was measured via Qubit and library size via the TapeStation. Multiplexed libraries were pooled and sequenced on a NovaSeq 6000 (Illumina, San Diego, CA) at 25X coverage using 2x150 paired-end sequencing