title: CUT&RUN of MCF10DCIS cocultured with DCIS-D4 using AIB1 antibody, rep 3
design: DCIS-RFP and DCIS4-GFP cells were cocultured at a 4:1 ratio (DCIS-RFP:DCIS4-GFP) for 48 hours. Cocultures were washed and then changed to serum- and hydrocortisone- free media. Four hours later, cells were switched to culture media (containing horse serum and hydrocortisone) with the addition of 100 nM dexamethasone for one hour. Cell populations were then separated via FACS and the CUT&RUN Assay Kit (Cell Signaling Technology) was performed with 250,000 cells for each reaction.