title: Whole-genome sequencing of human clinical isolates from Queretaro Mexico
design: The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, followed by 5' and 3' adapter ligation. Alternatively, "tagmentation" combines the fragmentation and ligation reactions into a single step that greatly increases the efficiency of the library preparation process. Adapter-ligated fragments are then PCR amplified and gel purified. For cluster generation, the library is loaded into a flow cell where fragments are captured on a lawnof surface-bound oligos complementary to the library adapters. As all 4 reversible,terminator-bound dNTPs are persent during each sequencing cycle, natural competition minimizesincorporation bias and greatly reduces raw error rates compared to other technologies.