title: RNAseq of human PBMCS: Pt01 day 15
design: RNA was extracted using the AllPrep DNA/RNA kit. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.  After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis.  The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification and purification.