title: Transcriptomic Responses of Laser Capture Microdissected Nephrons from Rat Kidneys Exposed to Single administration of 5 mg/kg Cisplatin: 29 days study - second technical replicate of the RNA sequencing
design: Transcriptomic Responses of Laser Capture Microdissected Nephrons from Rat Kidneys Exposed to Single administration of 5 mg/kg Cisplatin: 29 days study - second technical replicate of the RNA sequencing
library_construction_protocol: The kidneys were fixed with formaldehyde and embedded in paraffined. Prior to the collection, the kidneys were sectioned with 8 um thickness in a PEN membrane for laser capture microdissection purposes. On the collection day, the sections were stained with H&E staining and the regions of interest were collected. The samples were collected until reaching a total of ~200000 micrometer square of the surface area. The collected samples were frozen at -80'C until sequencing process. All the processes were according to BioSpyder-BioClavis protocol. All the processes were according to BioSpyder-BioClavis protocol.