title: GSM8577704: effector Lung ILC2, 30ug papain, 3 days, ATAC-seq, replicate2; Mus musculus; ATAC-seq
library_construction_protocol: Female wild-type mice were used for library construction unless otherwise stated. Lung ILC2s (Lin-CD25+ST2+ cells) were sorted by flow cytometry (Beckman Coulter) respectively from naïve (as ILC2nav) or papain-treated (30 μg/day for 3 days) mice (as ILC2eff). ILC2mem were isolated from the lungs of wild-type mice challenged with papain (intranasal administration, 30 μg per day) for 3 days and rested for 2 months.Each biological repeat was pooled about 5×104cells from 2-4 mice. Library was constructed by TruePrepTM DNA Library Prep Kit V2 for Illumina® kit from Vanzyme (Nanjing, China). 1.2×Agencourt AMPure XP beads (Beckman Coulter) were used to purify the libraries.