title: GSM9539094: NEF,S1,snATACseq; Mus musculus; ATAC-seq
library_construction_protocol: LSKs, cKit+ and monocytes were sorted from exNef and exGFP treated mice. Single cells were encapsulated in Gel-beads in emulsion (GEMs) using the 10X Controller with a Chromium Single Cell 3' GEM Library and Gel Bead kit (Baker Heart and Diabetes Institute) Transcriptomic (snRNA) and genomic (snATAC) libraries were prepared by loading 11,500 nuclei per lane and following Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kits User Guide with all required proprietary kits and equipment including the 10xGenomics Chromium Controller (1000204, 10xGenomics), Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Kit (PN- 1000283, 10xGenomics), Chromium Next GEM Chip J Single Cell Kit (PN-1000230, 10xGenomics) and Dual Index Kit TT Set A (PN-1000215, 10xGenomics). Libraries were pooled evenly and paired-end sequencing on an Illumina NovaSeq 6000 was performed.