title: Transcriptome of Bacillus licheniformis on hydrolysis lignin
design: rRNA was removed from total RNA, and then cDNA was synthesized using hexamer primers. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and a bioanalyzer for size distribution detection.  After quality control, Illumina sequencing was performed based on Sequencing by synthesis approach.