title: RNA-seq of E0771: mix mammary tumor
design: One million cells were injected bilaterally into the mammary fat pad of 8 week old female C57BL/6 mice. The three categories of mice were as follows: E0771 pLX304-empty vector overexpression, E0771 pLX304-AIB15 overexpression, and 80% E0771-EV + 20% E0771-5 (E0771-Mix).  Primary tumors were removed on days 28 and 31. Total RNA was extracted from mouse mammary gland tumors using RNeasy Mini Kit and MagNA Lyser Green Beads with the MagNA Lyser. RNA was sent to Novogene Corporation Inc. and RNA-seq libraries were prepared using the TruSeq Total RNA library Prep Kit (Illumina). 150 nucleotide paired-end sequencing was performed on NovaSeq 6000 S4.