title: GSM8655936: Model group rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from aortic arch tissue using TRIzol reagent. Tissue was ground in liquid nitrogen, mixed with TRIzol, and incubated at room temperature for 15 minutes. After chloroform addition and centrifugation, RNA was precipitated with isopropanol, washed with 75% ethanol, and dissolved in RNase-free water. 1.5 μg RNA per sample was used for library preparation using NEBNext® UltraTM RNA Library Prep Kit for Illumina®. mRNA was purified, fragmented, and reverse transcribed to cDNA, followed by second-strand synthesis, end repair, and adaptor ligation. After size selection and USER enzyme treatment, PCR amplification was performed.