title: Metagenomics of barcoded B. theta population: experiment E1 mouse 8 at day 12 from feces
design: DNA was extracted using PowerSoil HTP 96 kits (for homogenized fecal pellets, ceca, and small intestines) and UltraClean 96 microbial kits (for in vitro cultures) [Qiagen]. DNA was added to a tagmentation reaction for 10 min at 55 C before neutralization. In a 10-cycle PCR, adapter regions with TruSeq unique dual indices [Illumina] were attached to permit pooled sequencing and demultiplexing. Clean up on pooled libraries was performed with Ampure XP Beads [Beckman Coulter] prior to sequencing.