title: Repeated Measures Bulk RNA-Seq Analysis in Interictal Migraineurs vs. Healthy Controls
design: Repeated Measures Bulk RNA-Seq Analysis in Interictal Migraineurs vs. Healthy Controls
library_construction_protocol: Whole blood samples were harvested in PAXgene Blood RNA tubes (Qiagen, Venlo, The Netherlands) and snap frozen. PAXgene Blood mRNA kit (Qiagen, Venlo, The Netherlands) was used with  QiaCube instrument at Institute of Psychiatry, Psychology & Neuroscience, King's College London (United Kingdom) according to the manufacturerâ€™s instructions.  The NEBNext Ultra Directional RNA Library Prep Kit for Illumina was employed for sample processing. Sample preparation adhered to the \"NEBNext Ultra Directional RNA Library Prep Kit for Illumina\" protocol (NEB #E7420S/L).