title: Ovis dalli dalli (40071) long-read WGS: male muscle
design: 5 g of unsheared genomic DNA was used for library construction with the Pacific Biosciences Express Template Prep Kit version 2.0 (Pacific Biosciences, Menlo Park, CA). A post-library construction sizing step, i.e., the Blue Pippin system (Sage Science, Beverly, MA), was also included to remove small, degraded fragments. Sequencing was performed on the Sequel II sequencer (Pacific Biosciences) in the continuous long read (CLR) mode. We used four flow cells and a 15-hour movie acquisition time. The PacBios P filter step removed low-quality reads and adaptors from the raw reads.