title: 2022_Rosebud_Top_P_LTC11
design: gDNA extraction was completed using ThermoFisher MagMax DNA Ultra 2.0 with Cell and Tissue Extraction Buffer isolation kits on KingFisher robotics. Genomic DNA quantification was achieved using ThermoQuant-It assay kits and normalization using Beckman Echo acoustic liquid handling system, also with KingFisher robotics. Library preparation was completed using SbfI enzyme digest followed by ligation of multiplex RAD adaptors.