title: GSM9109926: Plate3_TF_KLF4_P3C4_dose0.5_rep1; Homo sapiens; RNA-Seq
library_construction_protocol: At 48 hours post transfection, we removed the media, added 32uL Trizol to the cells in each well to extract RNA, mixed thoroughly by pipetting, snap froze in dry ice + EtOH and stored the plate at -80C until ready for RNA purification. After thawing the Trizol prepped cells on ice, we used the Zymo Direct-zol-96 RNA kits (Zymo #R2056) to purify RNA. For RNA library preparation, we sent the total RNA to Novogene for bulk unstranded mRNA library preparation with PolyA enrichment and sequencing on an Illumina NovaSeq X Plus (paired end 150 cycles).