title: Genome sequencing of Cereus jamacaru: adult root
design: High-molecular-weight DNA (~20 g) was sheared to 1520 kb using the Megaruptor 3 (Diagenode). A PacBio HiFi library was prepared with the SMRTbell Express Template Prep Kit 2.0 and size-selected on a Pippin HT system (Sage Science) with an 810 kb cutoff. The final library was quantified using the Qubit HS kit (Invitrogen) and assessed for fragment size on the Femto Pulse System (Agilent). Sequencing was performed on the PacBio Sequel II platform using the Sequel II Binding Kit 2.0 and Sequencing Kit 2.0. The library was loaded onto three 8M SMRT cells and sequenced in Circular Consensus Sequencing (CCS) mode with 30-hour runs per cell.