title: GSM8690875: DQGD128; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA from seeds was extracted according to the protocol (Meng and Feldman 2010) except for the last precipitation performed at -80 °C for 1 hour. RNA from flowers was extracted with TRI Reagent (EUROMEDEX, TR118-100) following the manufacturer's protocol. Total RNA was then purified using the RNeasy MinElute Cleanup kit (Qiagen, 74204). Total RNA was treated with DNase I (Thermo Fisher Scientific, EN0521) and purified using the RNeasy MinElute Clean-up (Qiagen, 74204). RNA-seq libraries were then produced from 500 ng of DNase-treated RNAs using the TruSeq Stranded Total RNA Library Prep Plant (Illumina, 20020610) according to manufacturer's instructions. Sequencing was performed on an Illumina NextSeq 2000 system by the GenomEast platform (paired-end sequencing, 2 × 50 bases).