title: GSM8996849: male, WT/WT, Snord116+/+Ctg-/-, ZT03 biol rep 6, 12:12 L:D entrainment, circadian atlas; Mus musculus; RNA-Seq
library_construction_protocol: RNA were isolated from 10 - 20 mg prefrontal cortex using All Prep DNA/RNA/Protein kit (Qiagen). RNA was submitted for library preparation and sequencing to Novogene. Libraries were prepared using NEBNext Ultra II Directional RNA library prep kit for Illumina. Ribosomal RNA was cleaned up using NEBNext rRNA Depletion kit. Libraries were sequenced on the Novaseq S4.