title: GSM8514762: sup-tRNA, Total RNA, Biol Replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: 48 h after transfection cells were lysed in buffer (20 mM Tris pH 7.4, 100 mM NaCl, 10 mM MgCl2, 100 ug/mL cycloheximide, 1% (w/v) Triton X-100, 25 U/ml Turbo DNase I) and passaged through a 25-gauge needle six times. Insoluble material was removed by centrifugation and total RNA was extracted from the soluble material using miRNeasy Kit (Qiagen). Ribosome protected fragments were prepared using a sucrose cushion following RNAse I digestion as described previously (PMID: 33377062). Libraries were prepared as described previously (PMID: 26876183)