title: Microbiome of Tomato field microbiome
design: Genomic DNA was extracted from 150 to 200 mg of cecal contents using the PureLink Microbiome DNA Purification Kit (Life Technologies, Invitrogen Corp.), combined with RNAse treatment (10 units/hr). After quality control with electrophoresis and nanodrop,  the 16S rRNA V4-V5 variable region was amplified, purified, and sequenced. Amplicon libraries were prepared by using Phusion High-Fidelity PCR Kit (New England Biolabs Inc, Ipswich, MA, USA). PCR products were cleaned using AMPure XP PCR (Beckman Coulter Inc, Beverly MA, USA) and sequenced using Illumina MiSeq 300-base paired-end kit at the Molecular and Cellular Imaging Center (https://mcic.osu.edu/)