title: scRNAseq of macaca mulatta: Bone marrow cells differentiated into osteoclast
design: Bone marrow samples obtained through the Monkey Alcohol Tissue Research Resource (www.matrr.com) were from controls and macaques that engaged in very heavy drinking throughout the 12-month ethanol exposure. The bone marrow mononuclear cells were incubated in MEM supplemented with M-CSF overnight.  Non-adherent cells were then collected and re-plated with MEM supplemented M-CSF and RANKL. The media was replaced every 48 hours. 24 hours after the third media change, the culture supernatant was discarded, and the cells were collected and labeled with hashtag oligonucleotides (Biolegend cat #: 394641, 394643, 394645).