title: SMART-Seq of untreated and auxin-treated blastocysts for OGT degradation
design: SMART-Seq of untreated and auxin-treated blastocysts for OGT degradation
library_construction_protocol: Embryos were generated through IVF, then cultured in a standard mammalian cell incubator in KSOM medium. Modified Smart-Seq2 protocol using SuperScript IV RT and tagmentation procedure previously described (Hennig et al. 2018).