title: GSM9539124: MCF-7 NFκB-, 10Gy IR + TNF-ɑ, 4h, rep4; Homo sapiens; RNA-Seq
library_construction_protocol: In time-points post-treatment of interest, cells were lysed in 500 µL of TRIzol reagent (Thermo-Fisher, cat. 15596018) to extract the total RNA. Lysates were then purified using silica membrane columns (NucleoSpin RNA Plus, Machery-Nagel, Carlo Erba, cat FC140955N), thus extracting the RNA that was quantified and tested for purity by NanoDrop fluorometer (Thermo-Fisher) Sequencing libraries were prepared using the Standard Input mRNA library preparation protocol for NovaSeq6000, generating 1×100 nt single-end reads, and sequenced on the Illumina NovaSeq 6000 platform (Illumina, San Diego,450CA, USA).