title: 20 rep HEK vs. HeLa comparison HeLa R2
design: For HEK293 and HeLa transcriptional comparison, cells were cultured and maintained in an incubator at 37C and 5% CO2. HEK293 and HeLa cells were cultured in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% FBS, 1% penicillin-streptomycin, and 1% L-glutamine. Once the cells were confluent, the medium was removed and cells were washed once with 1 mL DPBS. The cells were resuspended with 0.5 mL Trizol and collected for Trizol RNA isolation.