title: 16s rRNA-seq of human gut metagenome
design: DNA from a ZymoBIOMICS mock community was extracted, quality-checked, and amplified targeting 16S rRNA hypervariable regions using one or two primer sets from the Ion 16S kit. Libraries were prepared, indexed, quantified, normalized, and sequenced on an Ion GeneStudio S5 Plus platform. Base calling and demultiplexing were performed with Torrent Suite, and a 100% E. coli sample was included as a positive control. 1S files contain reads from HVRs 2, 4,  and 8. 2S files contain reads from HVRs 2, 3, 4, 6-7, 8, and 9. HVR-2 files contain HVR 2 from samples sequenced with 1S primer set. HVR-4 files contain HVR 4 from samples sequenced with 1S primer set.