title: GSM9386526: D7_CTX_BM; Mus musculus; RNA-Seq
library_construction_protocol: Live cells were enriched using the EasySep Dead Cell Removal kit (Stemcell Technologies). Equal numbers of cells from three individual mice under each condition were mixed and the pooled samples were used for downstream sequencing. Bone marrow cells were stained with anti-CD11b and anti-Ly6C antibodies and sorted for CD11b+Ly6Chi monocytes by Bigfoot Spectral Cell Sorter. The purity of sorted monocytes was greater than 98%. scRNA-seq libraries were generated using 10X 3' single cell mRNAseq V3 reagents and sequenced using an Illumina NovaSeq 6000 sequencer.