title: GSM8514777: Rep1_IWRR, 1day; Bos taurus; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent according to the manufacturer's instructions. RNA concentration was determined using a Qubit 2.0 Flurometer. All samples passed the RNA integrity tests assessed with an Agilent 5400 Bioanalyzer. Total RNA was subjected to mRNA purification using poly-T oligo-attached magnetic beads. The RNA sequencing library was constructed using NEBNext Ultra RNA Library Prep Kit following the manufacturer's protocol. After synthesis of the first and second strand cDNA, library fragments were purified by AMPure XP system.