title: GSM8724527: HFF cells, H3K9me3, Mock, rep1; Homo sapiens; OTHER
library_construction_protocol: Cells were resuspended in Trizol (Invitrogen) and RNA extracted from Trizol. RNA-sequencing libraries were constructed using commercial kits (New England Biolabs) total RNA-seq with rRNA depletion as indicated; CUT&RUN was performed according to the CUT&RUN: Targeted in situ genome-wide profiling with high efficiency for low cell numbers V.3 protocol.