title: GSM8549792: MV411_RNA_JS8-100p_mouse3-0_240411_GTCCGC_S6; Homo sapiens; RNA-Seq
library_construction_protocol: Mouse cell depletion was performed on whole bone marrow cells using the Mouse Cell Depletion Kit and LS columns (Miltenyi Biotec). For RNA isolation, 1x10^6 human cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturers instructions for cell ine experiments. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA).