title: PS15m_Input
design: RNA pol II sample, 150-bp paired-end reads. Overnight, YPD broth cultures were processed for ChIP as described in previous research. Briefly, cultures were crosslinked, lysed, and chromatin sonicated throughout 35 cycles 30 s ON/OFF in a Bioruptor (Diogenode) to obtain 100-300 bp chromatin fragments. Sheared chromatin aliquots were stored as input DNA controls. ChIP-grade antibodies -H3K9me2 (ab1220, Abcam) and -RNA pol II (39497, Active Motif), as well as protein A or G magnetic beads accordingly, were utilized to immunoprecipitate chromatin-bound DNA. After washing and decrosslinking, DNA was purified by a phenol/chloroform-based extraction method. Libraries were prepared using Roche KAPA HyperPrep Kits and sequenced with Illumina NovaSeq X Plus sequencing system for 150-bp paired-end reads.