title: GSM8943230: cut_19_Fam114a1_KD; Mus musculus; RNA-Seq
library_construction_protocol: Primary tumors from control (shCoo2), FAM114A1-KD#1, and FAM114A1-KD#2 Py8119 cell inoculated mice were collected and processed into formalin-fixed paraffin-embedded (FFPE) tumors. Six control FFPE tumors were combined into one group termed as shCoo2 group. Three FAM114A1-KD#1 FFPE tumors and three FAM114A1-KD#2 FFPE tumors were combined into the FAM114A1-KD group. shCoo2 and FAM114A1-KD FFPE samples were thawed and divided equally for processing using the standard 10X Flex assays, following the respective recommended protocols. Single-cell RNA sequencing was performed with 10X Chromium Single Cell Gene Expression Flex on the Illumina Novaseq 6000.