title: GSM9436112: CSDS mouse hippocampus RNA-seq_MG4; Mus musculus; RNA-Seq
library_construction_protocol: For each group (CG, MG, and ME), six mice were randomly selected. Hippocampal tissues were rapidly dissected, snap-frozen in liquid nitrogen, and stored at −80 °C. Total RNA was extracted using the MJZol total RNA extraction kit (Majorbio, Shanghai, China) according to the manufacturer's instructions. RNA concentration and purity were assessed with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA), and RNA integrity was evaluated using an Agilent 5300 Bioanalyzer (Agilent Technologies, USA). Only high-quality RNA samples with an RNA Integrity Number (RIN) ≥ 7 were used for library preparation. RNA-seq libraries were prepared using the Stranded mRNA Prep and Ligation protocol (Illumina, USA), including poly(A) RNA enrichment, fragmentation, cDNA synthesis, adaptor ligation, and PCR amplification. Libraries were sequenced on the Illumina NovaSeq X Plus platform.