title: RNA-seq of 67NR: relacorilant mammary tumor
design: 200,000 cells were injected bilaterally into the mammary fat pad of 8 week old female BALB/c mice. All mice were injected with 80% 67NR parental + 20% 67NR5 mixed cells. Relacorilant, 30 mg/kg, or vehicle control treatment started one week after cells were injected. Drug was administered via oral gavage every Monday, Wednesday and Friday. Primary tumors were removed on Day 21. Total RNA was extracted from mouse mammary gland tumors using RNeasy Mini Kit and MagNA Lyser Green Beads with the MagNA Lyser. RNA was sent to Novogene Corporation Inc. and RNA-seq libraries were prepared using the TruSeq Total RNA library Prep Kit. 150 nucleotide paired-end sequencing was performed on NovaSeq 6000 S4.