title: GSM8762040: EaR2 L R3; Erwinia amylovora; RNA-Seq
library_construction_protocol: Cells were pelleted (10,000 xg, 2.5 min), rinsed with PBS, and cell pellets treated with RNAprotect Bacteria Reagent (Qiagen, Valencia, CA) diluted in PBS according to manufacturer's instructions. Treated cell pellets were stored at -80 ºC until use. RNA was extracted according to Rivas et al (2001), and cleaned and concentrated according to Kharadi et al (2022). RNA yield and quality was measured via spectrophotometry and agarose-gel electrophoresis. RNA libraries were prepared at Cornell's TREx facility, using NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs, USA) and NEB Next Ultra II directional Library Prep kit(New England Biolabs, USA) for Illumina (1000 ng total RNA) and sequenced on Illumina NovaSeq Sequencer, producing 2 x150 nucleotide paired-end reads.