title: skin microbiome of wild Rhinella marina at Middle Point, NT, Australia
design: Wild toads were hand-captured and sampled at night to detect temporal patterns of skin bacterial microbiomes and compare with toads housed in semi-natural captive enclosures. Toad was rinsed with milli-q water before entire body was swabbed with Copan flocked swab avoiding the face and cloaca. Sample was stored in 300uL Zymo DNA/RNA Shield and refrigerated until DNA extraction with Norgen's Microbiome kit. Amplicons of the bacterial V4 region of 16S rRNA gene were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.