title: GSM8690474: WT Tumor_1; Mus musculus; RNA-Seq
library_construction_protocol: For sc-RNA sequence, tumors were excised and minced into 1–2 mm fragments in a sterile petri dish.  The tissue fragments were digested in a buffer containing 10 mM HEPES, 1.25 mg/mL collagenase D, 0.85 mg/mL collagenase V, 30 U/mL DNase I, 1 mg/mL dispase II, and 10% fetal bovine serum (FBS) in Hank's Balanced Salt Solution (HBSS). Digestion was carried out on a rotator at 37°C for 45 minutes. Post-digestion, the cell suspension was filtered sequentially through 70 µm and 40 µm cell strainers (BD) to remove debris and large aggregates. The filtered cells were centrifuged at 600×g for 5 minutes at 4°C to pellet the single-cell suspension. The resulting suspension was used for single-cell RNA-sequencing. Gene expression libraries were aligned to the mm10 genome using Cell Ranger 5.0.1 (10X Genomics) with default parameters to generate gene-cell count matrices.