title: GSM9141221: GBM12_PBS 2; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Rneasy Plus kit from Qiagen and samples were submitted for bulk RNA sequencing Library preparation was performed with the SMARTer® Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc., cat# 634878). This process incorporated RiboGone™ and SMART® (Switching Mechanism at the 5' end of RNA Template) technologies, along with PCR amplification, to generate Illumina-compatible libraries without adapter ligation. RiboGone efficiently depleted rRNA from total RNA before library construction. Library quality was assessed using the TapeStation 2000 (Agilent Technologies), and concentrations were measured with Qubit (Thermo Fisher Scientific) before sequencing. Dual-indexed i5/i7 paired-end libraries were sequenced on the NovaSeq 6000 system (Illumina) with a minimum coverage of 50-60 million reads per sample.