title: Transcriptomics: water (negative control)
design: Negative control (PCR grade water). Total RNA nucleic acid was extracted by Qiagen RNAeasy Mini Kit. RQ1 RNase-Free DNase for DNA removal. rRNA depletion by Low Input Ribominus Eukaryote System v2. cDNA synthesis by SuperScript Double-Stranded cDNA Synthesis kit. Libraries were prepared using Nextera XT DNA Library Preparation kit.