title: RNA-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice
design: RNA-seq of quadriceps muscle tissues from 10 months old, 30 months old, 30 months old calorie restricted, 30 months old rapamycin treated adult mice
library_construction_protocol: Mice were sacrificed using CO2 inhalation and muscle were extracted and snap freezed immediately. All procedures were performed in accordance with Swiss regulations for animal experimentation, and approved by the veterinary commission of the Canton Basel-Stadt. Treatment was with rapamycin at 4.2 milligram per kilogram per day Hot phenol extraction of RNA SMARTerÂ® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian