title: GSM8942450: IFNg01; Homo sapiens; RNA-Seq
library_construction_protocol: NKX2-1GFP+ cells were sorted by FACS (FACS Aria; BD Biosciences) and lysed directly into 700 µl of Qiazol lysis reagent (Qiagen). Total RNA was extracted using the miRNeasy Micro Kit (Qiagen) following the manufacturer's instructions. RNA quality was verified using the Bioanalyzer 2100 (Agilent) and the RNA 6000 Nano Kit. RNA libraries were constructed using the Ovation Solo RNA-seq System (NuGEN) according to the manufacturer's instructions. cDNA libraries were indexed, quantified using the Quant-iT PicoGreen Kit (Life Technologies), and fragment size was verified using the Bioanalyzer 2100 (Agilent) with the DNA 1000 Kit. Libraries were multiplexed and sequenced on the Illumina HiSeq 1500 system (high-output mode, 2 × 100 bp).