title: GSM9262865: fars1_RNAi_2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. Synchronized L4-prefertile wild-type worms under control or fars-1 RNAi conditons were maintained on OP50 at 20°C, and harvested after washing three times independently using M9 buffer. Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. A sequencing library was constructed and paired-end RNA sequencing using Illumina NovaSeq 6000 platform was performed (Macrogen, South Korea).