title: DNA-seq of Escherichia coli isolated from patients with urinary tract infection isolate 8
design: Ribosomal RNA was depleted using NEBNext rRNA Depletion Kit (Bacteria) (New England Biolabs, # E7850). Libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, #E7760). DNA-seq libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England Biolabs, #E7805L). Finally, RNA-seq and DNA-seq libraries were sequenced on the NovaSeq X platform (Illumina).