title: Small RNA-Seq analysis of alfalfa leaves
design: Briefly, 3 and 5 adaptors were ligated to 3 and 5 ends of small RNAs. Then the first strand cDNA was synthesized after hybridazition with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between 18-40 bp were ready for sequencing on Illumina sequencing with SE50.