title: GSM9684776: Colonic eosinophils isolated from water (control)-treated C57Bl/6 WT mice,1,D6; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNA extraction kit (GenDopt) according to the manufacturer's instructions. RNA-seq libraries were prepared by Novogene Co., Ltd. (Beijing, China). Briefy, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. The final library was obtained after adapter ligation and PCR enrichment, and sequenced on the [Illumina NovaSeq X Plus] platform.