title: 18S ITS1-ITS2 amplicons from Schizaphis graminum
design: DNA was extracted using the DNeasy Blood and Tissue Kit for DNA Isolation (Qiagen, Germantown, MD) following its instructions.  PCR was performed with primers ITS1F (CTTGGTCATTTAGAGGAAGTAA) and ITS2R (GCTGCGTTCTTCATCGATGC).  The libraries were barcoded, pooled, and sequenced on an Illumina Novaseq 6000 to generate 2x250-base paired-end reads.