title: GSM9538444: NUGC4,WT,rep2,WGBS; Homo sapiens; Bisulfite-Seq
library_construction_protocol: Genomic DNA was extracted from approximately 100,000 cells. Purified genomic DNA was fragmented to an average size of 300–500 bp using a Covaris S220 sonicator. Fragments were purified with 1.6× SPRI Select beads. Libraries were constructed following standard Illumina protocols, including end repair and adenylation, and EM-seq™ methylated adapters were ligated. Bisulfite conversion was performed according to the EZ DNA Methylation-Gold Kit protocol (Zymo Research, USA). The resulting single-stranded DNA fragments were PCR-amplified using the KAPA HiFi HotStart Uracil+ ReadyMix (Roche Diagnostics) and purified.