title: GSM8577124: Control6Right; Mus musculus; RNA-Seq
library_construction_protocol: Single, live synovial CD45+ cells were isolated by flow cytometry sorting and subsequently analyzed by scRNA-seq. Approximately 1000 cells/µL were loaded into an eight-channel microfluidic chip, which encapsulated them with gel beads (GEMs) and barcoded with a unique molecular identifier (UMI) using 10xGenomics Single Cell 3′ reagent kit following manufacturer instructions (10xGenomics, Pleasanton, CA). A single-cell RNA-seq library from each biological replicate was run independently on Illumina NovaSeq flow cell with 26x98 bp reads and generated approximately 38,000-1,000,000 mean reads per library capturing 700-16000 cells per mice (see details in sTable 3).