title: GSM8577690: SH-SY5Y cells SGC707 C; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the MJzol Animal RNA Isolation Kit (Majorivd) following the manufacturer's standard protocol. The extracted RNA was then purified using the RNAClean XP Kit (Beckman Coulter) and treated with RNase-Free DNase to remove residual genomic DNA. RNA integrity was assessed using either the Agilent 2100 Bioanalyzer or Agilent 4200 TapeStation (Agilent Technologies). RNA concentration and purity were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific) and the NanoDrop ND-2000 Spectrophotometer (Thermo Fisher Scientific). Subsequent mRNA isolation and fragmentation were performed on the purified total RNA, followed by first- and second-strand cDNA synthesis. The cDNA was subjected to end-repair, 3′-end adenylation, adaptor ligation, and enrichment to construct the mRNA sequencing libraries by VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina®. Library concentrations were quantified using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific), and fragment size distributions were assessed using the Agilent 4200 TapeStation (Agilent Technologies). Sequencing was performed on an Illumina NovaSeq 6000 platform in paired-end 150 bp mode (PE150).