title: GSM9316398: Ripk1KO NIT-1 beta-cells TNF_IFN replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from NIT-1 CTL and Ripk1Δ beta-cells treated with vehicle or TNFα+IFNγ. RNA integrity was assessed prior to library preparation according to sequencing core facility guidelines. RNA-seq libraries were prepared from total RNA by the institutional sequencing core using a stranded mRNA-seq workflow with poly(A) selection following standard protocols. Libraries were barcoded, quantified, pooled, and sequenced on an Illumina NovaSeq 6000 as paired-end 2×100 bp reads.