title: Microbial communtiy composition of Illes Pond X
design: A part of the 16S rRNA gene was amplified with fusion primers containing Bacteria-specific sequences Bakt_341F (5'-CCT ACG GGN GGC WGC AG-3', Herlemann et al. 2011 ISME J) and modified Bakt_805R (5'-GAC TAC NVG GGT ATC TAA TCC-3', Herlemann et al. 2011 ISME J; Takahashi et al. 2014 Plos One), and Archaea-specific A519F (5'-CAG CMG CCG CGG TAA-3' and Arch855R (5'-TCC CCC GCC AAT TCC TTT AA-3') (Klindworth et al. 2013 Nucleic Acids Res) with Fluidigm CS1 and CS2 universal sequences at their 5' ends. Eukaryotic primers 574*F (5'-CGGTAAYTCCAGCTCYAV-3') and 1132R (5'-CCGTCAATTHCTTYAART-3') (Hugerth et al. 2014 Plos One) were used for amplifying part of the 18S rRNA gene fused with the same Fluidigm CS1 and CS2 adapters. The resulting PCR products were mixed before sequencing in 1:1:1 ratio.