title: Control samples
design: DNA isolated using the Qiagen DNeasy Powersoil Kit (cat# 12888). Libraries made with a modified Illumina 16S Meta-genomic Sequencing Library Preparation workflow, amplifying the v3/v4 of the 16S rRNA gene. Final libraries, approximately 630 base pairs (bp) in length, pooled in equal amounts based on fluorescence quantification, and quantitated via qPCR. Pooled library sequenced on an Illumina MiSeq using the MiSeq reagent kit v3 600 cycle chemistry (cat# MS-102-3003, Illumina, Inc., San Diego, CA, USA) at loading density of 68 pM with 20% PhiX, generating roughly 20 million, 300 bp paired-end reads.