title: Amplicon_seq of water_15:short_term
design: primer pair 27_BCtail-FW and 1492R_BCtail-RV; 5 extension for the barcoding PCR step; PCR amplification using the Phire Tissue direct PCR Master Mix; Multiplexing using the PCR Barcoding Expansion Pack 1-96; Library preparation using the sequencing kit SQK-LSK109; 48h sequencing run using a R9.4.1 flow cell