title: GSM8656208: KB2; Rattus; RNA-Seq
library_construction_protocol: After the total RNA samples were tested and qualified, they were purified according to the characteristics of mRNA from different sources. Eukaryotic mRNA has a polyA tail structure at the 3' end, and magnetic beads with Oligo (dT) are used for enrichment and purification. Add Fragmentation Bufer to the purified mRNA to make it fragmented into short fragments. The fragmented RNA is then used as a template for reverse transcription using random primers to synthesize the first strand of cDNA, and the second strand of cDNA is synthesized by adding 2nd Strand Marking Bufer and 2nd Strand/End Repair Enzyme Mix. After end repair, addition of base A, addition of sequencing connector, recovery of target fragments by magnetic bead screening, and PCR amplification, thus completing the entire library preparation.