title: GSM9315950: RNA-Seq, Tetraodon ventral retina, biol rep4; Tetraodon nigroviridis; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Invitrogen_ThermoFIsher). mRNA enriched librraies were preparaed and sequenced at Novogene (https://www.novogene.com/us-en). Briefly, for each sample, 1 µg RNA was used to generated RNA sequencing librarires using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB,USA), follwing the manufacturee's recommendation.