title: GSM8657088: G0_UNxSD-1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 10 mg of frozen mice kidney tissue samples utilizing the Qiagen RNeasy kit (catalog 74106) in accordance with the manufacturer's instructions. The integrity of RNA was rigorously assessed using the Agilent Bioanalyzer RNA 6000 Pico kit (Agilent Technologies, 5067-1513), with only samples having an RNA integrity number (RIN) of at least 6 being utilized for subsequent cDNA synthesis. For library preparation, the NEBNext Ultra RNA Library Prep Kit for Illumina (catalog E7530L) was utilized, following the manufacturer's protocol meticulously. After library construction, RNA-Seq libraries underwent sequencing to achieve a depth of 20 million 2 × 150 paired-end reads.