title: SHIV cDNA amplicon transcripts: rhesus macaque blood plasma
design: cDNA synthesized from viral RNA with SuperScript III Reverse Transcriptase, forward and reverse env region primers GCATGGAATAAAACTTTACAAGCGGT and CCACTTTATATTTATATAATTCACTTCTC (HXB2 reference NT coordinates 7233-7257 and 7661-7689) used, sequenced with an Illumina MiSeq 500V2 Nano kit with 20% PhiX spike-in