title: whole genome sequencing of cydonia oblonga: cultivar Fontenay
design: High molecular weight (HMW) DNA extraction: DNA was extracted from leaves using the protocol provided by Oxford Nanopore. DNA purity and concentrations were measured using the DeNovix QFX Fluorometer (DeNovix Inc.). HMW DNA size-range distribution was assessed using Agilent Genomic DNA ScreenTape Assay for 4150 TapeStation System (G2992AA, Agilent). Whole-genome long-read sequencing:  Libraries preparation and sequencing of Fontenay HMW DNA extractions were performed at MIGALs genomic center using ONT Ligation sequencing kit V14 (LSK-114) and ONT Ultra-long sequencing kit V14 (ULK-114) with a PromethION P2 Solo sequencing machine (Oxford Nanopore Technologies).