title: RNA-Seq of Cistanche
design: universal primers 27F (5' AGAGTTTGATCCTGGCTCAG 3') and 1492R (5' GGTTACCTTGTTACGACTT 3') synthesized by Beijing Dingguo Changsheng Biotech-nology Co., Ltd. were used. The re-inoculated and purified single colony was used as the DNA template to prepare a 30 L reaction system: 15 L of Taq enzyme, 11 L of ddH2O, 1 L (10 mol/L) of upstream primer, and 1 L (10 mol/L) of downstream primer. Then, a PCR amplification reaction was performed with the following program: initial denaturation at 94C for 5 min; denaturation at 94C for 30 s, annealing at 54C for 30 s, and extension at 72C for 45 s, for 35 cycles; and a final extension at 72C for 7 min.