title: GSM8723707: LPBrain，rep1; Larimichthys polyactis; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol (Invitrogen, USA) according to the manufacturer's instructions. The concentration of RNA was assessed using a NanoDrop (Thermo Fisher Scientific, USA), and the quality was evaluated on an Agilent 2100 bioanalyzer (Thermo Fisher Scientific, USA). All samples with an RNA integrity number (RIN) ≥ 7.0 were used to prepare sequencing libraries to ensure high-quality sequencing data. mRNA was purified using oligo (dT)-attached magnetic beads and fragmented into small pieces. cDNA was synthesized using random hexamer-primed reverse transcription. Subsequently, A-Tailing Mix and RNA Index Adapters were added and incubated for end repair. The cDNA fragments obtained from the previous step were amplified by PCR, and the double-stranded PCR products were heated, denatured and circularized by the splint oligo sequence to obtain the final library.