title: GSM8549729: mouse2_BMDM_β2AGO_LPS; Mus musculus; ATAC-seq
library_construction_protocol: Library prep was performed according to Buenrostro et al., 2015 (Curr Protoc Mol Biol. 109:21.29.1-21.29.9). Fifty thousand BMDM were centrifuged and washed with cold PBS. The cell pellet was resuspended with cold hypotonic lysis buffer. The lysate was centrifuged and the supernatant was discarded. The crude nuclei were treated with the transposition reaction mix containing Tn5 transposase (Nextera) and incubated at 37ºC for 30 minutes. DNA clean up was performed with the Qiagen Minelute PCR Purification Kit. The transposed DNA was PCR with primer 1 and barcode primer 2 at non-saturating conditions. The amplified DNA was size selected after agarose gel electrophoresis and only fragments ranging from 100bp to 1000bp were recovered. DNA purification from the gel was performed with the Qiagen QIAquick Gel Extraction Kit.