title: NGS Pigeon tissue
design: TRIzol-based RNA extraction kit (GeneAll Hybrid-R, South Korea), in accordance with the manufacturers instructions. The extracted RNA  was subjected to cDNA synthesis using SuperScript-III First-Strand Synthesis System (Invitrogen/Thermo Fisher Scientific). The Respiratory Virus Research Panel and Ion AmpliSeq library kit Plus (Invitrogen/Thermo Fisher Scientific) for library preparation. The sequencing was performed on Illumina platform