title: GSM8724691: WT_54 after 2 hours extended light at ZT10 in short day entrained 12-day seedlings; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Total RNA was isolated from whole plant tissue using the Qiagen RNeasy Plant Mini Kit. Residual genomic DNA was removed using the Invitrogen Turbo DNA-free kit, according to the manufacturer's protocol. Libraries were prepared with the NEBNext Ultra II Directional Library Prep Kit for Illumina, using the NEBNext poly(A) magnetic isolation module.