title: GSM9262390: FD AML, Control, 2, Input; Mus musculus; ChIP-Seq
library_construction_protocol: At the end of the treatment protocol cells were crosslinked using 1% formaldehyde for 10 minutes and quenced with the addition of 0.125M glycine. Nuclear preparation was preforming using the truCHIP Chromatin Shearing Kit (Covaris) and chromatin was sonicated using a S220 ultrasonicator (Covaris) for 5 minutes. Immunoprecipitation for H3K27ac (Abcam 4729). ChIP-seq libraries were preparied using Illumina libary preparation kit.