title: GSM8606136: Steer 3; Bos taurus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent (Zymo Research, Irvine, CA) and the Direct-zol RNA MiniPrep Plus Kit (Zymo Research) Total RNA was extracted and fragmented, followed by first-strand cDNA synthesis using random hexamer primers. Second-strand cDNA synthesis was performed using a dUTP mix. The resulting double-stranded cDNA was purified using the AMPure XP system. After end repair and adaptor ligation, the adaptor-linked cDNA was again purified with the AMPure XP system and enriched through 10 cycles of PCR. The final cDNA library was purified using the AMPure XP system, pooled, and paired-end sequenced on an Illumina platform.