title: GSM8944123: ENT_TCR; Mus musculus; OTHER
library_construction_protocol: For each treatment group, representative 100mg sections from three individual tumors were pooled and processed. Single cell suspensions were obtained using the mouse tumor dissociation kit (Miltenyi Biotec) and CD45+ cells were isolated with murine TIL isolation kit (Miltenyi Biotec), both according to the manufacturer's instructions. Cell counts were determined with AOPI and isolated cells were resuspended in PBS+0.04% BSA at a concentration of 1.8E6 cells/mL. 200µL of each cell solution was provided. Single-cell RNA sequencing with TCR sequencing was performed using 10X Genomics CellRanger v 7.1.0 according to 10X Genomics 5' v2 Single Cell User Guide. Cell viability was assessed via LunaFx7 (Logos Biosystems) and was >90% for all samples. Sequenced reads were aligned to the 10x Genomics provided mouse reference sequences (refdata-gex-mm10-2020-A for GEX and refdata-cellranger-vdj-GRCm38-alts-ensembl-5.0.0 for TCR).