title: GSM8944361: IDH1R132H immortalized human astrocytes, siRUNX2, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-Seq, samples were seeded in triplicates and harvested 48 days after the last treatment with Doxycycline and CHIR99021, Repsox or YO-01027 as described above. For RNA-Seq with siControl/siRUNX2 samples were harvested after 72h. RNA was isolated with the Rneasy Mini Kit (Qiagen). UltraLowRNAv4, NEBNext Multiplex Oligos for Illumina UDI. RNA-Seq TruSeq Stranded for sicontrol/siRUNX2.