title: GSM8968419: WT DNs GRO-Seq 2; Mus musculus; OTHER
library_construction_protocol: (B220-, CD19-, CD11b-, CD11c-, NK1.1-, TER119-, TCRβ-, TCRγ/δ-) DN thymocytes were sorted from Rag1-/- mice. Following the isolation of nascent RNA, the Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus Kit (20040525) with the IDT for Illumina RNA UD Indexes Set A (20040529) were used for library prep minus the ribosomal RNA depletion steps.