title: GSM8761655: Imaginal wing discs, HpoC, Rep3, NovaSeq X Plus; Drosophila melanogaster; RNA-Seq
library_construction_protocol: RNA was harvested using ReliaPrep RNA Tissue Miniprep System (Promega). RNA-Seq libraries were prepared with 10ng of total RNA cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags. Fragments were sequenced on an Illumina NovaSeq X Plus for the functional domains and conserved region D, and NovaSeq 6000 for the conserved regions A, B, C, E, and F, using paired end reads extending 150 bases