title: GSM9573834: Immortalized Human Nasal Epithelial Cells, Negative control siRNA, SCR3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol. Sequencing libraries were generated using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus kit (Illumina Inc., San Diego, CA, USA) from 100 ng of total RNA, following the depletion procedure as instructed. Libraries were sequenced on a HiSeq1500 system (Illumina) in PE100 rapid mode and pooled in equimolar proportions to a final concentration of 10 nM. Library concentrations were quantified using a Qubit 3.0 fluorometer (Invitrogen, Waltham, MA, USA), and fragment size distributions were verified by capillary electrophoresis on a Fragment Analyzer (AATI).