title: GSM9316604: CM-PEG-PLGA@DOX/R848-treated 4T1 cells replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol reagent. RNA integrity was confirmed using an Agilent 2100 Bioanalyzer. Sequencing libraries were prepared following the standard BGI mRNA library prep protocol, which includes mRNA enrichment, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification.