title: Genome Sequencing
design: Library preparation using KAPA Hyper Plus Kit , verified with DNA HS assay kit and Tapestation 4150. FastQC v.0.11.9 for quality assessment, raw reads were preprocessed using Fastp v.0.23.4  and processed using FastQC. Unicycler v.0.4.4, Multi-CSAR for de novo and reference based assembly . CheckM v.1.1.3 and QUAST for completeness and statistical quality assessment. BAKTA tool and other standard databases for functional annotations.