title: Amplicon sequencing: eukaryotic V4 region of 18S rRNA gene
design: PCR used primers TAReuk454FWD1 - TAReukREV3 (Stoeck et al. 2010) including an overhang for second PCR to add Illumina dual indexing adapters (Kozich et al. 2013); PCR used Promega GoTaq PCR Core Systems reagents