title: RNAseq CowRumen  Rep 4 Week 4
design: RNA-seq analysis of bovine rumen papillae tissue collected from 11 Holstein cows during dietary transition from low-grain (40% concentrate) to high-grain (65% concentrate) feeding. Samples were collected at three time points: baseline low-grain feeding (week 0), one week after high-grain transition (week 1), and four weeks after transition (week 4). The study aimed to characterize temporal transcriptional dynamics of rumen epithelial adaptation to high-grain diet and identify molecular signatures associated with subacute ruminal acidosis (SARA) susceptibility using paired-end Illumina sequencing