title: GSM9574556: WT_CRISPR_sgRosa26_female_6months_rep4; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from tissue using a bead homogenizer with TRIzol and Direct-zol RNA MiniPrep Plus kits (Zymo R2072). PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module and barcoded libraries were made using the NEBNext® Ultra II™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA).