title: RNAseq of Drosophila melanogaster: embryo stage 13
design: Each sample incubated  for 5-10 minutes at room temperature in TRIzol and was then frozen at -80 C. Total RNA was extracted using the TRIzol/Phenol-chloroform protocol. Total RNA was used for cDNA library construction using the TruSeq kit with PCR (Illumina, CA, USA) (conducted at the University of Chicago genomics core facility). cDNA libraries were barcoded and multiplexed for 100bp paired-end sequencing on one lane of a HiSeq Illumina 2000 sequencer.