title: sGff culture Nanopore sequencing
design: HMW DNA was extracted using the PureGene kit (Qiagen, Hilden, GER). An ONT LSK14 library was prepared following standard protocols and sequenced on a ONT 10.4.1 minION flow cell for 12 hours. Generated POD5 signal data files were basecalled with Dorado v0.9.1 using the SUP duplex pipeline. Simplex reads were dropped with Samtools v1.18, and remaining sequencing adapters were trimmed using Dorado trim. The produced FASTQ files were quality filtered with nanoq v0.10.0  to have a minimum quality of q10 and minimum 3000 bp read length.