title: RNA-seq Analysis in Huntington's disease (HD) peripheral blood mononuclear cell samples
design: RNA-seq Analysis in Huntington's disease (HD) peripheral blood mononuclear cell samples
library_construction_protocol: RNA analysis on peripheral blood mononuclear cells (PBMCs) was performed by RNA-seq in 30 subjects, including 15 HD subjects and 15 controls, all of Sicilian origin. RNA was extracted using Qiasymphony PAXgene blood RNA kiT. Indexed libraries were prepared from 700ng/ea purified RNA with Illumina Stranded mRNA (mRNA-Prep-Ligation) Library Prep,Ligation Kit according to the manufacturerâ€™s instructions. For library quantifications, TapeStation 4200 (Agilent Technologies) and Qubit (Thermo Fischer) was used.