title: RNA-seq of ERB macrophages
design: Total RNA was extracted using the QIAGEN RNeasy Mini Kit. Extracted RNA samples were submitted to Novogene for library preparation, quality control and sequencing. Messenger RNA (mRNA) was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, the first strands of complementary DNAs (cDNA) were synthesized using random hexamer primers, followed by the second cDNA strand synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification. After final quality control, cDNA libraries were sequenced on multiple lanes using the Illumina NovaSeq X Plus platform.