title: Day225SiteCC2
design: RNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (E7765, New England Biolabs, USA) following the manufacturer's instructions with the following options: 7 minutes fragmentation time, 5-fold Adapter Dilution, and seven cycles of final amplification. The libraries were run in a TapeStation 4150 with the D1000 Screentape and reagents to check for insert size distribution and the presence of adapter-primer dimers. After verification, the libraries were pooled equimolarly and sequenced on a NextSeq 500 (Illumina) using the 300 cycles, v2.5 (151bp paired-end sequencing) chemistry.