title: CUT&RUN of MCF10DCIS-D4 with GR antibody: rep 2
design: DCIS and DCIS4 cells were plated in cell culture plates. The following day, cells were washed and changed to serum- and hydrocortisone- free media. Four hours later, cells were switched to culture media (containing horse serum and hydrocortisone) with the addition of 100 nM dexamethasone for one hour. A total of 250,000 cells were collected for each reaction and the CUT&RUN Assay Kit (Cell Signaling Technology) was performed according to manufacturers protocol.