title: 16S rRNA targeted sequencing Lake Cadagno sediments at 582 cm
design: Sediment samples were collected from various depths (3 cm to 738 cm) in Lake Cadagno, Switzerland, in August 2019. DNA was extracted from the anoxic, sulfidic sediment samples, and the V4-V5 hypervariable regions of the 16S rRNA gene were amplified using the primer pair 515F/926R (Fuhrman and Parada). The amplified products were sequenced using high-throughput sequencing