title: GSM9468901: Overexpression of gene atp8 in plant 1 and overexpression group 2; Medicago sativa; RNA-Seq
library_construction_protocol: Transcriptome sequencing was performed by Wuhan MetWare Biotechnology Co., Ltd. using a standard plant mRNA-seq workflow. Anther samples from wild-type (WT) plants and two atp8 overexpression lines (atp8-OE1 and atp8-OE2) were collected, with three independent biological replicates per genotype. Total RNA was extracted following standard protocols, and RNA quality was assessed prior to library construction. RNA sequencing was conducted by Illumina platforms based on sequencing-by-synthesis (SBS) technology.