title: GSM9386637: control oocyte [CO3]; Mus musculus; RNA-Seq
library_construction_protocol: SMART-seq v4 Ultra-low input RNA kit The single-cell SMART-seq2 RNA-seq libraries were constructed using a modified single-cell tagged reverse transcription sequencing protocol. Briefly, mRNA from each single cell was extracted into the lysis buffer provided by the SMART-seq v4 Ultra-low input RNA kit (634891, TaKaRa, San Jose, CA, USA). mRNAs were then reversed transcribed into complementary DNA（cDNA), which was then pre-amplified and purified. Single-cell transcriptomes were subsequently tagged with unique barcodes using the Nextera XT DNA library preparation kit (FC-131-1096, Illumina, San Diego, CA, USA) and the Nextera XT Index kit v2 setA (TG-131-2001, Illumina).