title: ambient environmental control microbiome at Middle Point, NT, Australia
design: Ambient control samples were collected during toad sampling, with swab exposed to the ambient air, milli-q water, and glove to identify and remove contaminant reads from toad microbiome data. Sample was stored in 300uL Zymo DNA/RNA Shield and refrigerated until DNA extraction with Norgen's Microbiome kit. Amplicons of the bacterial V4 region of 16S rRNA gene were prepped and sequenced on MiSeq at Ramaciotti Centre for Genomics.