title: mRNA-Seq of primary MEFs untreated or treated with auxin for OGT degradation
design: mRNA-Seq of primary MEFs untreated or treated with auxin for OGT degradation
library_construction_protocol: Primary MEFs derived from E12.5 embryos were treated with auxin (IAA) for different amounts of time or left untreated. Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB). The integrity of the RNA was determined using the TapeStation 4150. Library preparation was performed using the TruSeq Stranded mRNA Library Preparation kit (Illumina) according to the manufacturer's instructions.