title: GSM8863037: HUDEP-2_CTCFAID_C3_2h; Homo sapiens; OTHER
library_construction_protocol: RNA was extracted using RNeasy Plus Mini Kit (Qiagen 74134). For nascent RNA labeling, 2 μg of total RNA was subjected to a click reaction with biotin azide and CuSO₄. Ribosomal RNA was depleted using the NEBNext rRNA Depletion Kit (NEB E7400X), and RNA library preparation was performed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770).