title: saline tilapia replicate 2
design: Genomic DNA (gDNA) extracted by TIANamp Genomic DNA KIT (Tiangen, China) using manufacturers protocol. DNA concentration and purity were quantitatively evaluated with nanodrop spectrophotometer and qubit fluorometry, and stored at 80 C until used for subsequent steps. V3-V4 16SrDNA hypervariable region was targeted to be amplified by PCR using 341F (5 -CCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTTCTAAT-3) primers pair. Successful amplification was confirmed through Agarose Gel Electrophoresis. Amplicon library preparation and sequencing were performed in PT. Genetika Science, Indonesia. Amplicon library prepared using Illumina MiSeq DNA PCR-Free Sample Preparation Kit (Illumina, USA) using manufacturer protocol. The library's quality was evaluated using a Qubit 2.0 Fluorometer (Thermo Scientific, USA) and an Agilent Bioanalyzer 2100 system (Agilent, USA). Amplicon library sequenced using an Illumina platform (Illumina, USA) to generate 250 bp paired-end reads in FASTQ format.