title: GSM8943887: OVCAR5 Pt-S OA n1; Homo sapiens; RNA-Seq
library_construction_protocol: The RNA was extracted using the QIAGEN RNeasy Mini Kit according to manufacturer's instructions. The RNA was quantified using a NanoDrop 3300 Fluorospectrometer. The RNA-sequencing (RNA-seq) libraries were prepared from 1 μg of total RNA of OVCAR5 Pt-S/Pt-R, starvation and supplementation with 50 uM OA/PA. Libraries were generated using the NEBNext Ultra II RNA library prep kit for Illumina (#E7770S, New England Biolabs, Inc.). Library preparation was performed accoding to manufacter's protocol.