title: GSM8577354: PEO1 B, G4 CT, Rep2; Homo sapiens; OTHER
library_construction_protocol: Cells were fixed with 0.1% formaldehyde for 2 minutes before being quenched with glycine. The CUT&Tag protocol was then followed, using either h3k27ac antibody or BG4 to target h3k27ac or G-quadruplexes resepctively. Tagmented DNA was purified using the Zymo DNA clean and concentrator-5 kit. Libraries were prepared using Illumina sequencing primers (i5 and i7 barcodes), and amplified on a thermocycler. Amplified DNA was extracted and cleaned using Ampure XP paramagnetic beads and the fragment size distribution was analysed using Agilent high sensitivity D1000 screentape.