title: GSM8514472: replicate2 pAKT mAb83-14 bin4; Mus musculus; OTHER
library_construction_protocol: Used conventional phenol-chlorophorm methode to isolate gDNA Plasmid library for PacBio sequencing was constructed using saturation mutagenesis, and library for sequencing barcodes from gDNA constructed using PCR primers tagged with p5 and p7 illumina sequencing primers Amplicon sequencing by PacBio and illumina sequencing of PCR products from gDNA