title: GSM9538460: Model_rep2_IP; Rattus norvegicus; RIP-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol, and RNA quality was evaluated using NanoDrop ND-1000 and agarose gel electrophoresis. Total RNA was randomly fragmented to ~200 nt. Protein A/G beads were coupled to the m6A antibody, and the RNA fragments were incubated with the bead-linked antibodies at 4°C for 4 h. RNA libraries for IP and Input samples were then constructed with the GenSeq Low Input Whole RNA Library Prep Kit by following the manufacturer's instructions.