title: RNA-seq of Cladocopium C1: 20 C control rep1
design: Total RNA was extracted from Symbiodinium cultures. Algae were detached by pipetting and the suspension was mixed thoroughly. One hundred milliliters of algal culture were collected in portions and centrifuged at 8000 rpm for 10 min, after which the supernatant was discarded. The algal pellets were transferred into 2 mL tubes, resuspended in PBS buffer, and centrifuged again at 8000 rpm for 3 min. After removing the supernatant, RNA stabilizer was added to the pellets and the samples were stored at -20 C. All samples were then sent to Sangon Biotech (Shanghai, China) for transcriptome sequencing.