title: RNA-Seq of wenyingzhuangia fucanilytica: Glu
design: RNA quality was confirmed using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). mRNA was purified using the Illumina Ribo-Zero rRNA Removal Kit (Bacteria) and subjected to fragmentation by the addition of a fragmentation buffer. di-tagged cDNAs were synthesized through a series of random priming reactions with terminal-tagging oligonucleotides, followed by purification using AMPure XP beads (Beckman Coulter, Beverly, MA, USA). Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer, and a final mRNA sequencing library was constructed.