title: GSM9615231: Cut&Tag-seq H3K4me1 in D5  with GSKJ4; Mus musculus; OTHER
library_construction_protocol: Cells were harvested at indicated stages for Cut&Run assay using Hyperactive pG-MNase Cut&Run Assay Kit for Illumina (Vazyme HD102), for Cut&Tag assay using Hyperactive Universal Cut&Tag Assay Kit for Illumina Pro (Vazyme TD904) according to manufacture's protocols. Regarding Cut&Run-seq,  VAHTS multiplex Oligos Set 5 (Vazyme N322) was used for library construction. As for Cut&Tag-seq, Trueprep Index Kit V2 for Illumina (Vazyme TD202) was employed for library construction. For RNA-seq, First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. After adenylation of 3' ends of DNA fragments, Adaptor with hairpin loop structure were ligated to prepare for hybridization. Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Prime and lastly PCR products were purified.