title: mRNA-seq of conjugating E. vannus: 12 hours post mixing
design: Briefly, the mRNA sequencing libraries were generated using NEBNext Ultra RNA library prep kit for Illumina (Cat. E7530, NEB) following the manufacturers instructions. Prior to mRNA library preparation, poly (A)+ RNA was isolated using magnetic beads coupling of Oligo d (T) (Cat. N401-01/02, VAHTS mRNA Capture Beads, Vazyme, China).