title: GSM8761493: non-diseased, patient ND178; Homo sapiens; RNA-Seq
library_construction_protocol: Single nuclei suspensions were prepared from approximately 50-100 mg frozen heart left ventricle tissues. Tissues were first cut into tiny pieces using scissors in ice-cold nuclei extraction buffer (Miltenyi Biotec) and dissociated in a gentleMACS Octo Dissociator. The resulting suspensions were centrifuged at 300 x g, strained in 70 µm MACS SmartStrainers, and washed in additional nuclei extraction buffer. Nuclei were then pelleted at 300 x g and gently resuspended in 1 mL ice-cold resuspension buffer (PBS, 0.1% BSA, 0.2 U/µL RNAse inhibitor). Initial cDNA libraries were prepared according to the 10X Genomics Chromium Single Cell 3' v3.1 protocol with a target of 3,000 nuclei per sample. The cDNA PCR amplification elongation step was extended to 3 minutes and fragmentation and subsequent steps were not performed. Full-length Nanopore cDNA libraries were prepared according to the Oxford Nanopore Technologies SQK-LSK114 kit.