title: RNA-Seq of tumor tissue from P9280216
design: Total RNA was extracted from tumor tissue using TRIzol Reagent (Invitrogen, cat. no. 15596026) following standard protocols. RNA was treated with DNase I to remove genomic DNA contamination. RNA quality and concentration were assessed using a Nanodrop OneC spectrophotometer and a Qubit 3.0 fluorometer. RNA integrity was confirmed by 1.5% agarose gel electrophoresis. Stranded RNA-seq libraries were prepared from 2 g of total RNA using the KC-Digital Stranded mRNA Library Prep Kit for Illumina (DR08502, Wuhan Seqhealth), which incorporates unique molecular identifiers (UMIs) to reduce PCR and sequencing duplication bias. Libraries of 200500 bp were size-selected, enriched, and sequenced on the DNBSEQ-T7 platform (MGI) using 150 bp paired-end reads.