title: RNA-Seq of mus musculus: adult male iWAT
design: This study aimed to investigate the transcriptomic effects of chronic social isolation on adipose tissue in mice. Inguinal white adipose tissue (iWAT) was collected from male C57BL/6J mice subjected to either standard group housing (control) or social isolation for an extended period. Each group consisted of 3 biological replicates (n=3). Total RNA was extracted and polyA-enriched libraries were prepared using standard protocols. Sequencing was performed on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads. The resulting data were used to assess differential gene expression between socially isolated and control mice.