title: DNA-seq of homo sapiens: adult female feces
design: Fecal samples were voluntarily submitted by participants, processed and frozen at -80 degrees Celsius, and then sent to the lab when ready for analysis. through the Indiana Biobank. Samples were processed at Microbiome Insights using a standardized shotgun metagenomic sequencing workflow. DNA extraction was performed using the Qiagen MagAttract PowerSoil DNA KF Kit with the Thermo Fisher KingFisher automatic extractor. Library preparation was conducted using the Illumina Nextera XT DNA Library Prep Kit, targeting 15 million paired-end reads per sample. Full shotgun sequencing was then performed on the Illumina NextSeq 500 platform.