title: 16S sequencing of anaerobic sludge: AnMBR
design: To sequence the V4 region of the 16S rRNA gene, two-step, Nextera barcoded PCR li-braries using the primer pair 515F (5- GTGYCAGCMGCCGCGGTAA -3) and 806R (5- GGACTACNVGGGTWTCTAAT -3) [14]. Subsequently, the PCR libraries were sequenced on an Illumina NovaSeq 6000 platform using an SP 500 cycles kit.