title: GSM9386552: ctrli_3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. Synchronized L4-prefertile wild-type worms under control or rnk-1 rnk-2 RNAi conditons were maintained on OP50 at 20°C, and harvested after washing three times independently using M9 buffer.For rnk-1 rnk-2 double RNAi experiments, the two double-stranded RNA (dsRNA)-expressing bacteria were separately cultured using liquid LB media containing 100 μg/mL ampicillin (USB, Santa Clara, CA, USA) at 37°C overnight until the optical density (OD) value at 600 nm reached 0.9. The bacteria were then mixed to 1:1 ratio. Total RNA was isolated using RNAiso plus (Takara, Japan) in accordance with the manufacturer's instructions. A sequencing library was constructed and paired-end RNA sequencing using Illumina NovaSeq 6000 platform was performed (Macrogen, South Korea).