title: Microbiome of B. pharaonis: winter rep2
design: DNAs were extracted from the whole soft tissues of B. pharaonis using the DNeasy Blood and Tissue Kit, and the 16S rRNA gene was amplified targeting the V4 region using 515f and 806r primers. Amplicons were sequenced on the Illumina MiSeq platform using a 2x250 bp paired-end configuration.