title: snRNA-seg atlas of Drosophila meconium-free female pupal midgut epithelium  during 8-12h APF (CRX1622320; CRA024664)
library_construction_protocol: Single-nucleus RNA sequencing libraries were prepared from 8-12h APF Drosophila pupal midguts using the 10x Genomics Chromium 3' v3 kit. Nuclei were isolated via mechanical homogenization and filtered through a 40 um cell strainer. cDNA synthesis and amplification followed the manufacturer's protocol. Final libraries were quantified by Qubit and sequenced on Illumina NovaSeq 6000.