title: RNA-Seq of rice seedling:heat stressed root
design: Total RNA was extracted from plant tissue using the PureLink RNA Mini Kit (Invitrogen) according to the manufacturers instructions. RNA quality and quantity were assessed using the Agilent 2100 Bioanalyzer. Approximately 2 micrograms of total RNA per sample were used for cDNA library construction. Paired-end libraries were prepared using the Illumina HiSeq2000 RNA Library Preparation Kit following the standard protocol provided by Illumina.