title: GSM9059627: KCT3266:mCAFs cells, C57BL6, EC359+Gem-2; Mus musculus; RNA-Seq
library_construction_protocol: RNA isolated from orthotopic tumors treated with vehicle, EC359, Gem, and EC359+Gem was analyzed by RNA sequencing (Novogene Co. Ltd., CA) using the Arraystar RNA Sequencing service, which includes library preparation, sequencing, quality control, and mapping (mapping statistic summary, transcript coverage) Briefly, mRNA was isolated from total RNA with NEBNext® Poly(A) mRNA Magnetic Isolation Module. Alternatively, rRNA is removed from the total RNA with a RiboZero Magnetic Gold Kit; The enriched mRNA or rRNA depleted RNA was used for RNA-seq library preparation using KAPA Stranded RNA-Seq Library Prep Kit (Illumina).