title: Sequencing of fecal metagenome
design: Sequencing of rumen microbiota in cows with different lactation days during postpartum period. Fecal samples from eight randomly selected cows per group were collected on days 1, 3, and 7 postpartum for metagenomic analysis. Genomic DNA was sheared using a Covaris M220 system (Gene Company, China). Approximately 350 bp fragments were selected, and metagenome shotgun sequencing libraries were constructed using the NEXTFLEX Rapid DNA-Seq kit (Bioo Scientific, USA). Subsequently, metagenomic libraries were sequenced via an Illumina NovaSeq X Plus system (Illumina, USA).