title: GSM8634110: LS9, Lung, Sham air inhalation, Rep7; Rattus norvegicus; RNA-Seq
library_construction_protocol: Tissue was harvested 24h after exposure. Euthanasia was performed via exsanguination under deep anesthesia (5% induction, 2% maintenance with isoflurane gas) followed by organ removal. Whole tissues  (brain and lung) were snap frozen in liquid nitrogen and stored at −80 °C. Lung and brain tissue was sent to the University of Minnesota Genomics Center (UMGC) for isolation of total RNA using an RNAeasy Plus Universal kit (Qiagen, Germantown, MD), according to manufacturer's instructions. Libraries were prepared by the UMGC. RNA was quantified using a RiboGreen assay (Thermo Fisher, Waltham, MA), and quality was assessed by capillary electrophoresis using a Tapestation (Agilent, Santa Clara, CA). All samples had an RNA integrity number (RIN) >= 8. Libraries were prepared using a TruSeq Stranded Total RNA Library Prep Kit (Illumina, San Diego, CA), and next-generation sequencing was performed using a Novaseq 6000 (Illumina) to generate 150 bp read pairs.