title: sequences of bacteria
design: Total DNA was extracted from rhizosphere soil (< 2 mm) using the DNeasy PowerSoil Pro Kit (Qiagen) following the manufacturers bead-beating and chemical lysis protocol. DNA quality was assessed by 1.0% agarose gel electrophoresis (80 V, 45 min) and a NanoDrop 2000 spectrophotometer (Thermo Scientific), requiring an A260/A280 of 1.82.0 and concentration  20 ng L1. The bacterial/archaeal 16S rRNA V3V4 region and the fungal ITS2 region were amplified by PCR. Amplicon size and specificity were confirmed on 2% agarose gels (120 V, 40 min), and target bands were purified with an AxyPrep DNA Gel Extraction Kit (Axygen). Equimolar amounts of purified amplicons were pooled and paired-end libraries were prepared with the Illumina TruSeq Nano DNA LT Library Prep Kit. Libraries were quantified with a Qubit 4.0 Fluorometer and sized on an Agilent 4200 TapeStation (target insert: 550650 bp), then sequenced (2  250 bp) on an Illumina NovaSeq 6020 (Guangxi Pufei Information Technology Co., Ltd.).