title: GSM9258915: Male-WT1; Mus musculus; ATAC-seq
library_construction_protocol: Frozen mouse SATs were sent to Active Motif for the ATAC-seq assay. They were initially ground to a fine powder using liquid nitrogen and resuspended in appropriate lysis buffer (Active Motif). Isolated nuclei were quantified using the Countess II FL automated cell counter (Thermo Fisher) and 100,000 nuclei were labelled as previously described (Buenrostro et al., 2013), with some modifications based on (Corces et al., 2017), using the enzyme and buffer provided in the ATAC-Seq kit (Active Motif). The labelled DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR and purified using Agencourt AMPure SPRI beads (Beckman Coulter).