title: GSM8513975: Expanded bispecific-secreting T cells, CD4+ and CD8+ starting material, donor 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from lysed cells using a RNeasy mini kit (QIAGEN) following the manufacturer's protocol. Bulk RNA sequencing was performed by Novogene using a non-directional library and a sequencing depth of 20M reads. Briefly, a non-directional library was prepared by purifying mRNA using poly-T oligo-attached magnetic beads, fragmenting, converting to cDNA, and PCR amplification after end repair, A-tailing, and adapter ligation. Libraries were sequenced using an Illumina platform and clean reads were processed.