title: GSM8577366: intestine, dorsal-GFP; Drosophila melanogaster; OTHER
library_construction_protocol: Female flies were harvested on the fifth-day post eclosion and 30 female midguts were dissected in PBS at RT. Then, single-cell suspension of midgut were prepared by trypsinization and filtration under asepsis condition. CUT&Tag libraries was performed with CUT&Tag reagent kit (CUT-01, Ruoyu Biotech) according to the manufacturer's instructions. To amplify the libraries, 16 μl of DNA was mixed with a uniquely barcoded i5 and a uniquely barcoded i7 primer, and amplified using 2×HG Library Mix. The libraries were purified with 1.2 × volume of  Ampure XP beads.