title: GSM9315350: Mbnl1 knockout; Clcn1 delta E7a mouse, P7, Quad tissue, Clcn1 amp from E1-E23, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from murine quadriceps tissue with Rneasy kit (Qiagen). Total RNA was reverse transcribed using SuperScript II (Invitrogen) with Rnase H treatment. Sequencing libraries were generated based on the PacBio protocol for multiplexing with barcoded universal primers designed to amplify Clcn1 transcripts from exon 1 to exon 23 (final exon). The remainder of library prep was completed per SMRTbell Express Template Prep Kit 2.0. The pooled libraries were diluted and submitted for sequencing on the PacBio Sequel system at the Molecular Biology and Genomics Core (Washington State University, Pullman, WA).