title: GSM8488325: fWT1_RNA; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from 20 mg of frozen liver tissue using the Quick-RNA Miniprep Kit (Zymo Research), following the manufacturer's protocol. RNA concentration was determined with the Qubit RNA Broad Range assay kit (Thermo Fisher), and RNA integrity was assessed using the Bioanalyzer 6000 Nano Chip (Agilent). RNA libraries were prepared using the NEBNext RNA Library Prep Kit for Illumina sequencing.