GSM9032520: Uncoated_catheter_biofilm_BioRep1; Pseudomonas aeruginosa PAO1; RNA-Seq
RNA was extracted using TRIzol reagent (Invitrogen, USA), following to the manufacturer's protocol, and resuspensed in RNase - free water. Ribosomal RNA was removed using TIANSeq rRNA Depletion Kit (G-bacteria) (TIANGEN, China), following to the manufacturer's protocol. The libraray was consturcted using Optimal Dual-mode mRNA Library Prep Kit (MGI, China), following to the manufacturer's protocol.