AE-2 (CRX1895853; CRA028665)
Total RNA was obtained using the Qiagen RNA extraction kit according to the manufacturer's instructions. DNA was removed using DNase treatment, followed by mRNA enrichment with magnetic beads. The enriched mRNA was then reverse transcribed into cDNA for library construction. Sequencing was conducted using the Illumina HiSeq 2500 at Novogene (China), generating approximately 2 Gb of data per transcriptome sample.