GSM9062660: 461-27304_14-Day_S2_OVCAR3_ROI-008_Secretory; Homo sapiens; OTHER
Formalin-fixed paraffin-embedded (FFPE) slides were prepared following the “RNA Slide Preparation Protocol” in MAN-10150 in the Manual Slide Preparation User Manual, available from NanoString. The RNA probe hybridization solution was prepared using the GeoMx® Cancer Transcriptome Atlas (CTA) RNA probe mix (NanoString), following the recommended dilution (10% probe mix + 10% DEPC water + 80% Buffer R). Library prep was performed according to instructions provided in NanoString's NGS Readout Library Prep User Manual (MAN-10117-05), followed by paired-end (27 x 27 ) sequencing on the Illumina NextSeq 550 sequencer using a high-output flow cell.