GSM8579773: ATAC_seq_MM196_MM_CELL_LINES; Homo sapiens; ATAC-seq
Cell lines were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% glutamine. Subsequently, the cell lines were washed twice with 1x PBS. ATAC-seq was performed in accordance with the Buenrostro protocol (Curr Protoc Mol Biol. 2015). Starting with 50.000 CD138+ plasma cells, library construction was performed using the hyperactive transposase Tn5. NGS adapters were pre-loaded onto the transposase, allowing simultaneous chromatin fragmentation and adapter integration into open chromatin regions.