GSM8728275: Sample 5, Location 3; Homo sapiens; OTHER
Tissue sections were mounted onto poly-L-lysine coated slides, deparaffinized, H&E stained, and imaged. One library per section was generated using the Visium CytAssist Spatial Gene Expression for FFPE kit according to the manufacturer's instructions (10X Genomics, Pleasanton, CA). Briefly, sections were destained and decrosslinked prior to probe hybridization overnight. Following hybridization, probe pairs were ligated and tissues restained with H&E and imaged on the CytAssist instrument. Sections were treated with RNase prior to tissue permeabilization. Slides were loaded onto the CytAssist where probe release and transfer to the capture area (6.5 x 6.5 mm) of the 10X Visium GEX slide were performed. Capture probes were extended, pre-amplified, and the resulting PCR product purified. Probe libraries were indexed in a final PCR reaction with 12-15 cycles, as determined by the qPCR, prior to cleanup. Spatial transcriptomics libraries were prepared using Visium V4 Slide - FFPE v2 chemistry following 10x Genomics protocols.