RNA-seq of CD8+ T cells from JSLE patients and healthy controls
RNA-seq of CD8+ T cells from JSLE patients and healthy controls
Peripheral blood was obtained by venipuncture, followed by PBMC isolation via Ficoll gradient. Cells were frozen in liquid nitrogen until use. Thawed PBMCs were sorted using FACS to select CD8+ T cells. PicoPure RNA extraction kit as per manufacturer's protocol TruSeq Stranded mRNA Library prep kit (Illumina cat. no. 20020595) OR KAPA mRNA HyperPrep Kit (Roche p/n KK8580), depending on sequencing batch