GSM9396584: LPS-1; Homo sapiens; RNA-Seq
Total RNA was extracted from the samples, the integrity of the extracted RNA was determined using agarose gel electrophoresis, and RNA concentration was determined using NanoDrop. Oligo-dT magnetic beads were used to isolate mRNA from total RNA, and the captured mRNA was fragmented. The first and second strands of cDNA were synthesised using RT, and end repair was performed with the addition of an A base at the 3' end. Subsequently, the ligation product was purified, and PCR amplification was performed. The sequencing library was purified using magnetic beads. The library concentration was detected using a Qubit instrument, while the fragment length was determined using an Agilent fragment analyser.