WT CD4T-3 (CRX1779769; CRA026860)
The purified mRNAs were cleaved into small fragments; First-strand cDNAs were produced by reverse transcription; Second-strand cDNAs are synthesized, while simultaneously performing end repair and dA-Tailing; Sequencing adapter ligation was performed, followed by DNA magnetic bead purification and fragment selection; The ligated products were amplified by PCR and purified again using DNA magnetic beads, where the products were solubilized with nuclear-free water.