GSM9062078: pooled library of all cell lines, cortical + subpallial organoids, day 150; Homo sapiens; RNA-Seq
Cell suspension was prepared from several pooled organoids that were chopped to smaller pieces, using papain dissociation followed by trituration in Low Ovo solution. The suspensions were fixed according to the Chromium Fixed RNA Profilling Sample Preparation kit (10X Genomics) instructions. Sequencing of the samples was performed by the Center for Translational Genomics (CTG) at Lund University, using the 10X Next GEM protocol and probe set version 1. Sequencing was done in two batches (D75 batch 1 and D150 batch 2), samples were multiplexed, labeled with barcodes, and split to multiple lanes.