GSM9176681: ms5-Par_ISO; Mus musculus; RNA-Seq
Parietal skull bone was homogenized in TRIzol and RNA was isolated using the RNeasy Mini Kit (Qiagen, Cat# 74104) following the manufacturer's instructions. RNA quality and integrity were assessed using 2200 TapeStation with RNA Screen Tape (Agilent Technologies). Library preparation and sequencing were performed by Macrogen using their standard bulk RNA-seq service. Briefly, 1 µg RNA was used as an input for the TruSeq Stranded mRNA Library Prep Kit (Illumina). Sequencing was carried out on an Illumina NovaSeq 6000 platform using paired-end 101 bp reads, with a throughput of 6 Gb per sample.