GSM9142539: IL-17Ai, Synovial tissue biopsie, SpA9 (PsA), treatment, week 12; Homo sapiens; RNA-Seq
Total RNA was extracted from the snap-frozen whole-tissue synovial biopsies using RNA Stat-60 (Tel-Test Inc), treated with DNase I (Invitrogen) and cleaned using RNeasy columns (79254, Qiagen) according to manufacturer's instructions. RNA concentration and integrity were measured using the Qubit RNA BR Assay kit (Life Technologies) and the Agilent 2100 Bioanalyzer (Agilent Technologies), correspondingly. cDNA libraries were constructed with Illumina TruSeq™ RNA Sample Preparation Kit (Illumina) using 1 µg of total RNA with RNA integrity number (RIN) ≥ 7.