GSM9579163: wt-3; Danio rerio; RNA-Seq
Extract total RNA using the Trizol method for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo_x0002_attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.After library quality control, quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.