GSM9690565: PDAC Normal FFPE Tissue; Homo sapiens; RNA-Seq
Total RNA was extracted from the macrodissected FFPE samples using the Promega Maxwell® RSC RNA FFPE Kit. RNA-seq libraries were prepared using a minimum 50 ng of purified RNA from FFPE material with a Takara PICO RNA input kit with SMART-seq2 protocol. The protocol included cDNA preparation using TSO oligos, cleanup, PCR amplification, and pooling of indexed libraries. Final libraries were then normalized to 4 nM. Library denaturation and dilution were performed following the Illumina NextSeq System Denature and Dilute Libraries Guide.