GSM9690294: A549 Cells, U2AF1 S34A, sample 4; Homo sapiens; RNA-Seq
RNA for each cell clone was extracted using the RNeasy Plus Mini Kit (QIAGEN, 74134) and quantified using the Qubit RNA Broad Range Quantification Assay Kit (Invitrogen, Q10210) Short-read RNA-sequencing was performed with Novogene. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. The samples were then processed by end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library quality was checked with Qubit and real-time PCR for quantification and BioAnalyzer for size distribution detection.