C8094Spleen (CRX959519; CRA014888)
The library fragments were purified and eluted with EB buffer, the terminal repair was performed, poly (A) was added, and the adapter was implemented. To preferentially select cDNA fragments 400 bp in length, the library fragments were separated by size by agarose gel electrophoresis, and then the 400 bp fragments were purified by gel extraction. The UNG enzyme was used to digest the second strand of cDNA. PCR was performed, the target products were retrieved by agarose gel electrophoresis, and the library was completed. The libraries were clustered and sequenced on an Illumina NovaSeq 6000 platform, and 150 bp paired-end reads were generated.