GSM8832712: Multiome Day 7 Control ATAC; Homo sapiens; ATAC-seq
100,000 cells (viability 95%) were lysed for 4min and resuspended in Diluted Nuclei Buffer (10x Genomics, PN- 2000207). Lysis efficiency and nuclei concentration was evaluated on Countess II automated cell counter by trypan blue staining.  9,660 nuclei were loaded per transposition reaction, targeting recovery of 10,000 nuclei after encapsulation. After transposition reaction nuclei were encapsulated and barcoded. Next-generation sequencing libraries were constructed following the User Guide, which were sequenced on an Illumina NovaSeq 6000 system at the same time.