GSM9145386: ischemia kidney HH1; Mus musculus; RNA-Seq
Total kidney RNA was extracted using RNeasy mini kit (Qiagen, Hilden, Germany) according to the manufacture's protocol. Library preparation and sequencing were performed by High Throughput Sequencing Facility at UNC Chapel Hill. Paired-end raw sequences of 100 bp were acquired by NextSeq 2000 (Illumina, San Diego, CA), and subsequent demultiplexing was performed using bclconvert with a 1-mismatch threshold to segregate reads based on indexes.