GSM9471774: IUGR/B6I3_PLA(raw); Mus musculus; miRNA-Seq
Total RNA was extracted from mouse placenta samples (with intact decidua layer) using a bead homogenizer and Trizol LS reagent (ThermoFisher, 10-296-028) /chloroform method. RNA concentration was measured using the NanoDrop 2000c spectrophotometer (Thermofisher Scientific). If necessary, an RNA clean and concentrator kit (Zymo Research R1019) was used to remove any contaminating DNA, phenol, and concentrate samples to improve 260/280 (1.9-2.0) and 260/230 ratios (2.0-2.2). Sequencing was performed by LC Sciences (Houston, Texas, USA). We used 1 µg of total RNA to prepare small RNA libraries with TruSeq Small RNA Sample Prep Kits (Illumina, San Diego, USA). Single-end 50bp sequencing was conducted on an Illumina Hiseq 2500 system.