GSM9031127: F1863_Heart_BCR; Mus musculus; OTHER
Single-cell suspensions from each heart tissue and matched PBMC sample were loaded into separate wells of a Chromium Next GEM Chip N (10X Genomics, Cat# 2000418) to generate gel beads-in-emulsion (GEMs), each containing uniquely barcoded beads for downstream single-cell capture and indexing. Cell concentration was adjusted to approximately 700-2,000 cells/µL to target recovery of up to 20,000 cells per sample. Sequencing libraries for 5' gene expression and T cell receptor (TCR) V(D)J profiling were prepared using the Chromium Next GEM Single Cell 5' HT Reagent Kits v2 (10X Genomics, Cat# 1000356), the Mouse Chromium Single Cell V(D)J Amplification Kits (10X Genomics, Cat#1000254) and the Library Construction Kit (10X Genomics, Cat# 1000190) according to the manufacturer's instructions. All libraries were sequenced on the Illumina NovaSeq 6000 system using paired-end reads with dual indexing 26 cycles for Read 1, 10 cycles for i7 Index, 10 cycles for i5 Index, and 90 cycles for Read 2), targeting 400 million reads per gene expression library and 100 million reads per TCR amplification library.