WGS of Marinobacter shengliensis isolate 2B from the Mariana forearc serpentinite mud volcanoes
For DNA extraction, microbial isolates were grown anaerobically in balch tubes with 7 mL of anaerobic, alkaline MJYTGL medium, following several rounds of dilution-to-extinction. The medium was not reduced prior to inoculation. Cultures were topped off with a headspace of 100% N2 after inoculation and incubated at 30C for 3-4 days, or until visibly turbid. Genomic DNA was extracted from a single culture from each isolate using the MasterPure Complete DNA and RNA Purification Kit, following manufacturers instructions, except with added overnight DNA precipitation. Samples were prepared for whole genome sequencing using the Illumina DNA Prep tagmentation kit following manufacturers instructions and IDT For Illumina Unique Dual Indexes. Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle flow cell kit to produce 2x150bp paired-end reads, with a sequencing coverage of 48.64 - 76.58x.