GSM9690353: Multiplexed sublibrary of all samples, whole-transcriptome poly-A capture; sublibrary 2; Homo sapiens; RNA-Seq
After recovery to 1e6 cells/well, cells were washed with PBS and dissociated with TrypLE. Dissociated cells were fixed with Parse Evercode cell fixation kits. Whole-transcriptome scRNA-seq libraries were generated from fixed cells with a Parse Evercode WT v3 kit. Single cell barcode sequencing libraries were generated from fixed cells with a Parse CRISPR-detect kit. Libraries were generated with a target of 2650 cells/sample for barcoded cells and 4150 cells/sample for unbarcoded cells. Both kits produced 8 sublibraries (WT and CR, respectively) for sequencing. WT libraries were split evenly across 3.2B reads and CR libraries were split evenly across 500M reads.