RNA-Seq of Cochliomyia macellaria : proboscis of female adult gravid day 7
Total RNA was isolated from whole tissues or specific life stages of whole individuals using a Monarch Total RNA Miniprep Kit (New England Biolabs). mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and the libraries made using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs). Paired-end libraries (2 x 150 bp ) were sequenced on an Illumina NextSeq2000 to help annotate the new genome assembly.