GSM9031102: PBMC, cohort 2,  Orai1 Mother; Homo sapiens; RNA-Seq
Collected cells were also quantified using a Bio-Rad TC20 automated cell counter. Stained cells were encapsulated into emulsion droplets using Chromium Controller (10x Genomics) for library prep. scRNA-seq and antibody multiplexing libraries were constructed using Chromium Single Cell 3′ v3.1 Reagent Kit and 3' Feature Barcode Kit (PN-1000268 & 1000262 respectively) according to the manufacturer's protocol. Individual libraries were diluted to 2nM and pooled for sequencing. Pooled batches were sequenced with 100 cycle run kits (28bp Read1, 8bp Index1 and 91bp Read2) on the NovaSeq6000 Sequencing System (Illumina).  Amplified cDNA was evaluated with an Agilent BioAnalyzer 2100 using a High Sensitivity DNA Kit (Agilent Technologies). Final libraries were evaluated with an Agilent TapeStation 4200 using High Sensitivity D1000 ScreenTape (Agilent Technologies).