GSM9543788: LN229,GFP,rep2; Homo sapiens; RNA-Seq
By Plasmidsaurus: Total RNA extracted using a bead-based extraction approach from cells preserved in Zymo DNA/RNA Shield. Post-extraction RNA concentration assessed using a fluorescence based plate reader assay. By Plasmidsaurus: mRNA converted into complementary DNA (cDNA) via reverse transcription and second-strand synthesis, followed by tagmentation, library indexing, and amplification. cDNA generated using a poly(dT)VN primer