16s rRNA sequencing of mus musculus: CKO_Ctrl_4
Fecal DNA was extracted by FastDNA Spin Kit for Soil (MP Biomedicals). Barcoded amplicons targeting the V3-V4 region were produced via PCR. Firstly, 10 ng of genomic DNA was processed for the PCR. Following amplification, PCR products were purified and quantified. These products were then multiplexed in equimolar amounts and sequenced on an Illumina MiSeq PE300 platform (2  300 bp).