wgs of cat with epidermolysis bullosa
Genomic DNA was isolated from fresh frozen tissue (DNeasy blood and tissue kit; Qiagen). Genomic DNA was quantified (Qubit 2.0 Fluorometer; ThermoFisher). NEBNext Ultra II DNALibrary Prep Kit (New England Biolabs) was used to prepare sequencing libraries, following themanufacturers recommendations. DNA libraries were quantified by real-time PCR (AppliedBiosystems). Paired-end 150-bp libraries were sequenced (NovaSeq X Plus; Illumina).