GSM8579588: siG1_1; Homo sapiens; RNA-Seq
RNA was isolated using the Zymo Direct-zol Mini-prep kit (Zymo-Catalog # RD2051 Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.