Whole-genome sequencing of A. thaliana: accession Dolna-1-40
High molecular weight (HMW) DNA was extracted from plant leaf tissue with CTAB-based cell lysis with RNase A and Proteinase K treatment. HMW DNA was precipitated on the gravity-based Genomic-tip 20/G columns (QIAGEN). HMW DNA was purified with ZYMO DNA Clean & Concentrator -10 (ZYMO RESEARCH). Sequencing libraries were prepared using adapter ligation kit from Oxford Nanopore Technology (LSK-SQK-109). The samples were sequenced on the MinION sequencer with the R9.4 nanopore flow cell version. Raw signals were basecalled to FASTQ format and adapters were trimmed using dorado.