GSM9032634: 10-E; Homo sapiens; RNA-Seq
Samples were enriched for live cells using FACS sorting prior to quantification and loading.  A single-cell suspension from each sample was quantified and analyzed for viability using the Nexcelom Cellometer K2 and then loaded onto the 10X Genomics Chromium Single Cell Controller for scRNA-seq library preparation (10X Genomics, Pleasanton, CA) The cDNA was amplified and purified, and cDNA libraries were then prepared in bulk reactions using the 10X Chromium Single Cell 3' Library Prep Kit.