GSM8578297: Non Affected, bird # NA68; Gallus gallus; RNA-Seq
Qiagen Rneasy Mini Kit Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification