tRNA_m1A_seq of Caenorhabditis elegans: Young Adult
tRNA fragments were subjected to partial hydrolysis using a Na2CO3/NaHCO3 buffer to generate 5'-monophosphate ends. Dephosphorylation with calf intestinal alkaline phosphatase (CIP) was followed by rephosphorylation using T4 polynucleotide kinase. Purified tRNA fragments were converted into barcoded small RNA libraries using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs). Library quality and concentration were assessed using an Agilent 2100 Bioanalyzer. Libraries were pooled in equal molar ratios and sequenced on the Illumina NextSeq 500 system.