16S-seq of ocean microbiome: Semakau, Singapore
Water samples were kept on ice and filtered within 8 hours, then stored at 80C prior to DNA extraction using the Qiagen PowerWater kit. Extracted DNA was sequenced at Azenta Life Sciences using Illumina MiSeq with 338F/806R primers targeting the V3V4 region of the 16S rRNA gene, generating 52K350K reads per sample.