shotgun metagenomic sequencing of Microalgae pond growing Scenedesmus obliquus UTEX 393
Pond water samples were collected from four open-air microalgae ponds in Mesa, Arizona, containing Scenedesmus obliquus UTEX 393 (three ponds) and Tetraselmis striata (one pond). The water was filtered sequentially through 3.0 m and 0.45 m mixed cellulose ester (MCE) filters, with the final flowthrough concentrated using an Innovaprep CP Select Ultra CPT system. The Qiagen PowerWater DNeasy kit was used to extract nucleic acids. Sequencing libraries were prepared using the Element Biosciences Elevate Enzymatic Library Prep kit and sequenced on an Element AVITI system with a Cloudbreak 2x150 Low Output kit.