WGS of Pseudobacillus badius
Total gDNA was used for the input of library preparation. Total gDNA was repaired using end prep enzyme mix, generating DNA with 5 phosphorylated, 3 dA-tailed ends. Barcodes were ligated with the blunt/TA ligase and then equal concentrations of the DNA was pooled. The repaired DNA was ligated with ONT-compatible Adapter. The library was quantified with Qubit Fluorometer before loading to the Flow Cell. Sequencing was performed using GridION (Oxford Nanopore Technologies) until the desired yield has achieved.