GSM9106212: HS294T_control biol rep 1; Homo sapiens; RNA-Seq
For RNA extraction, the culture medium was removed, and the cells were washed twice with 3 - 5 mL of PBS. After the final wash, 0.5 mL of TRIzol reagent was added, and the cells were scraped from the plate. The suspension was pipetted 12 - 15 times to ensure homogeneity and transferred to an RNase-free Eppendorf tube, which was labeled and stored at –80 °C. RNA libraries for RNA-seq were prepared using KAPA Stranded mRNAseq Kit following manufacturer's protocols.