GSM8607135: DCs_RT-iPSC_NHEJ; Homo sapiens; RNA-Seq
RNA extract and libraries were prepared for sequencing using standard protocols of HiSeq X Ten by Genewiz. ScRNA-seq libraries were generated using the 10X Genomics Chromium Single Cell 3' Kit v2 and the 10x Chromium Controller (10x Genomics) according to the 10X Single Cell 3' v2 protocol. Single cells, reagents and single gel beads containing barcoded oligonucleotides were encapsulated into nanoliter-sized droplets and subjected to reverse transcription. Droplets were broken and the barcoded cDNAs were purified with DynaBeads and amplified by 12 cycles of PCR. The amplified cDNA was fragmented, end-repaired, ligated with index adaptors, and size-selected with clean-ups between each step using the SPRIselect Reagent Kit. Quality control of the resulting barcoded libraries was performed with the Agilent TapeStation and by PCR with primers specific to the P5 and P7 sequence.