GSM8516756: Saseq_DF2_WT_5hr_1; Mus musculus; RNA-Seq
Primary neurons were collected in TRIzol (Invitrogen) and stored at -80°C overnight. Total RNA was isolated from each sample, and further RNA cleanup was performed using the RNeasy Mini Kit (Qiagen). The Dynabeads mRNA DIRECT Purification Kit (Invitrogen) was used to isolate mRNA from the samples. The NEBNext Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) was used, and the recommended protocol was followed to generate the library.