GSM8945944: LECs, CONV-R sample2; Mus musculus; RNA-Seq
Total RNA extracted from LECs were sequenced as described previously (REF DOI: 10.1016/j.isci.2021.103092) with the following modifications. RNA concentration and integrity were analysed using a TapeStation 4200 System (Agilent) and a Qubit 4 fluorometer (ThermoFisher Scientific). RNA libraries were prepared using TruSeq stranded mRNA Kit (Illumina) according to manufacturer's instructions. All samples were sequenced using an Illumina NovaSeq6000 sequencer (Illumina, San Diego,CA)