GSM9322425: SW620, CNT_3; Homo sapiens; RNA-Seq
used Rneasy mini kit for RNA extraction RNA samples were processed by Novogene using their standard Illumina-compatible stranded mRNA-seq workflow. Poly-A mRNA was enriched with oligo-dT beads, fragmented, and reverse-transcribed to synthesize first- and second-strand cDNA (dUTP method for strand specificity). After end repair, A-tailing, and ligation of indexed Illumina adapters, libraries were PCR-amplified, size-selected, and quality-checked using the Agilent Bioanalyzer/Tapestation before sequencing on the Illumina NovaSeq platform.