GSM9579020: VEEV TrD-infected primary mouse astrocytes, 16hpi, biol rep2; Mus musculus; RNA-Seq
Total RNA extracted from infected cells at 16 and 24hpi and from uninfected cells using Direct-Zol RNA Microprep Kit following the manufacturer's protocol. Ribosomal RNA was depleted using the RiboGone Kit according to the manufacturer's instructions. The RNA was prepared for sequencing using the SMARTer Universal Low  Input RNA Kit for Sequencing following the manufacturer's instructions. Samples were  then prepared using the Ion Plus Fragment Library Kit (Ion Torrent) at the UNM ATG core facility, starting at the Ligation and Nick Repair step, with the only modification being the use of 80% ethanol instead of 70% ethanol for bead washes.