GSM9214673: A2xA3_T_R3; Hordeum vulgare; RNA-Seq
RNA was extracted from the pooled second leaves of 10 plants per sample per biological replicate. RNA extraction was performed with hot-phenol RNA extraction as described ⁠. RNA samples were treated with DNAse I (Thermo Fisher 18047019) and DNAse was removed by performing another hot-phenol RNA extraction on the DNAse-treated samples. For qRT-PCR, DNAse-treated RNA was reverse-transcribed using SuperScript II (Thermo Fisher 18064014) according to manufacturer's instructions. RNA paired-end sequencing was performed at Novogene.