Susceptible strain, control R2
Libraries for whole transcriptome sequencing were produced from 20 ng total RNA per sample using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England 131 Biolabs) and its corresponding sample indexing kit (Index Primer Set 1). Finished libraries were assessed for quality prior to sequencing (a) via quantitative analysis using Qubit Broad Range assay kit, and (b) for library preparation size distribution via Agilent 4200 TapeStation. Libraries were then sequenced on the Illumina NextSeq 2000 platform using paired-end 2 x 150 bp sequencing reads with Illumina Stranded mRNA Prep kit.