Normal Diet with 10 percent Grapefruit rep 3
All animal experiments were performed within the Hebrew University of Jerusalems guidelines of the Authority for Biological and Biomedical Models and were approved by its Institutional Animal Care Ethics Committee (AG-24882-01). Forty-eight C57BL/6J male mice (6-7 weeks old) were purchased from Harlan Laboratories (Jerusalem, Israel). Mice were randomly divided into six experimental groups (8 mice in each group): normal diet (ND), normal diet + 10% (w/w) red grapefruit (ND+10%RGF), ND+15%RGF (w/w), HFD, HFD+10%RGF (w/w), HFD+15%RGF (w/w). Diet compositions are presented in Table 1 and Table 2. Mice were housed in a controlled environment (12/12 hours light/dark cycle, 20-24C) with ad libitum access to water. Food was changed daily, 50 gr each time. Mice were weighted once a week and food consumption was measured twice a week. Diet treatments lasted for 15 weeks.