RNA-Sqq of mus musculus Hippocampus
hippocampal tissue was homogenized in chilled Nuclei EZ Lysis Buffer (NUC101, Sigma-Aldrich | Merck, USA) using a glass dounce tissue grinder (FIS#K885300-0002, Kimble Kontes, USA). Homogenates were filtered through 70 m strainers, centrifuged at 500 g for 5 minutes at 4C, or cleansed via density gradient centrifugation with Debris Removal Solution (130-109-398, Miltenyi Biotec, Germany). Nuclei were resuspended, centrifuged, and passed through a 35 m strainer. Approximately 6000 nuclei per sample were captured using 10 Genomics Single Cell 3 Reagents kit. Sequencing was performed on an Illumina NovaSeq 6000 with 150 bp paired-end reads by Genechem (Shanghai).