GSM8657778: Hepatoblastoma sample 22; Homo sapiens; OTHER
Ten tissue sections with a maximum area of 6.5 x 6.5 mm2 were cut from ten distinct HB FFPE tissue bocks, placed on the mRNA capture areas of Visium Gene Expression slides for FFPE (10x Genomics, Visium v1), deparaffinized, and H&E stained according to the respective user guide (CG000409 Rev C, 10x Genomics). Twelve additional samples were processed using a CytAssist machine (10x Genomics, Visium v2), which transfers the transcriptomic probes from standard glass slides to spatially barcoded Visium slides, enabling more efficient capture of whole gene expression. For all samples, brightfield histology images were taken using a 4x or 10x objective on a Nikon Eclipse Ni. Raw images were stitched together using NIS-Elements (Nikon) and exported as TIFF files with high-resolution settings. Libraries were prepared following the manufacturer's user guide (G000407 Rev D, 10x Genomics).