GSM8944929: WT_ChipSeq_1; Mus musculus; OTHER
Mice were immunized with MOG/CFA to induce EAE. CD4+ YFP+ cells from spleen at the peak of disease were sorted on an AriaIII cell sorter into 15-ml tubes containing complete media, counted, and placed on ice. All samples were spun down at 500g for 10 min. The supernatant was removed, and cells were resuspended in 100 μl of 1 × PBS (Thermo Fisher Scientific). Cut & Tag libraries were prepared using Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme) following manufacturer's protocols.