count_ID_076403_female_36
The sample ID for the data used in this analysis, outcome for the patient and EBOV viral load (1/Ct) are summarised in Additional file 1: Table S1. For sequencing of the samples, RNA-seq libraries were prepared from the DNAse-treated total RNA using the Epicentre ScriptSeq v2 RNA-Seq Library Preparation Kit, followed by 10-15 cycles of amplification and purification using AMPure XP beads. Samples from 86.46% of the fatalities and 52.63% of the survivors were amplified with 15 cycles. Each library was quantified using Qubit and the size distribution assessed using the Agilent 2100 Bioanalyser and the final libraries were pooled in equimolar ratios.