GSM8865505: WT, 4kPa substrate, 24hr culture, rep2; Homo sapiens; RNA-Seq
Cells were lysed using TriZol (Thermo Fischer) and RNA isolated with Qiagen's RNeasy Micro Prep kit. RNA isolates with masses >= 0.5μg and RNA integrity number (RIN) scores >= 9.0 were used to create sequencing libraries. RNA libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina) according to the manufacturer's instructions.