16S amplicon seq of fungal: surrounding soil
DNA was extracted from 0.5 g of soil with the PowerSoil DNA Isolation kit (MoBio Laboratories, Carlsbad, CA, USA). After DNA concentrations were quality checked and quantified, all samples were stored at -80C until sequencing analysis. The library construction and sequencing of  16S gene were performed1. The universal primer sets, 341F and 806R targeting the V3 - V4 hypervariable regions of the bacterial 16S rRNA gene, was used in this study (Supplementary Data 7).