Metagenome of sweet fermented sticky rice (Khao-Mak)
Total bacterial DNA was extracted from three replicates of each sample by using a DNeasy food kit (Qiagen, Inc., Valencia, CA, USA). The DNA quantity and quality were determined using a DS-11FX+ spectro/fluorometer (DeNovix, Wilmington, DE, USA). The V4 variable region of the 16S rRNA gene was amplified, linked to the barcodes and Phusion high-fidelity PCR master mix (New England Biolabs, Ipswich, MA, USA). The PCR product was purified using a QIAquick gel extraction kit (Qiagen, Inc., Valencia, CA, USA). The amplified DNA library was constructed with a TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA) and analyzed with a HiSeq2500 PE250 sequencing platform (Illumina, San Diego, CA, USA).