High-throughput Cultivation and Identification of Bacteria from scarab beetle (CRX1858942; CRA027969)
DNAofsortedNcsCs was extracted from the cell line using a QlAamp DNA Mini Kit, sheared to approximately 300-500bp using a Covaris s220 instrument, Then the libraries were constructed through end-repair, A-tailing,adapter ligation and bisulfate-converted using a ZymoEZ DNA Methylation Kit.