GSM8465581: carbo_sham_d7; Mus musculus; RNA-Seq
enzyme-based mouse heart digestion, FACS, Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 from 10X Genomics. The Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 from 10X Genomics was used following manufacturer's instruction. For each experimental group, 10,000 cells were loaded into one well of a Chip G kit for GEM generation. The reverse transcription, barcoding, post-RT cleanup with Dynbeads, cDNA amplification, and purification with SPRIselect were performed according to the Chromium 10x 3'v1 protocol as the library preparation step. Each sample was sequenced on a NovaSeq 6000 S4 platform with a sequencing depth of approximately 20,000–30,000 reads per cell. Sequencing reads were aligned to the whole genome mRNA reference generated from the mm10 transcriptome using the Cell Ranger V3 software (10X Genomics) according to the 10X Genomics instructions. The count matrices were pre-processed and analyzed using R package Seurat (v3.2.3).