GSM8606886: SI_37331_Ms#3652_R265-71del+/+; Trp53f/f PbCre+_AD_Case; Mus musculus; OTHER
Single-cell multiomics was performed using the Chromium Next GEM Single-cell Multiome ATAC+Gene expression kit (10x Genomics) according to the manufacturer's protocol20. Briefly, nuclei were isolated, followed by transposition and adapter ligation using a Tn5 transposase. Transposed nuclei were loaded onto a Chromium Next GEM microfluidic chip along with gel beads containing 10x barcoded oligonucleotides. The GEMs were incubated to produce barcoded DNA from transposed chromatin (ATAC) and barcoded full-length cDNA from polyadenylated mRNA (Gene expression) within each GEM. Following this, the GEM is broken, cDNA is amplified, and pre-amplified cDNA is used as input for both the ATAC and gene expression library preparation. For ATAC, sample indexing primers are used for further amplification while the amplified cDNA underwent fragmentation, end repair, and A-tailing, followed by adapter ligation and PCR amplification to generate final sequencing libraries. Library quality was assessed using an Agilent Bioanalyzer.