GSM9061694: scRNAseq PT11_VDJ; Homo sapiens; OTHER
CITEseq+TCRseq: single cell RNA and TCR sequencing was performed on longitudinal peripheral blood immune cells collected from HR+ breast cancer patients treated with immune checkpoint inhibitors. Red blood cell lysis and magnetic bead dead cell removal was performed on cyroperserved PBMCs prior to RNA extraction and library preparation. Samples (targeting 15,000 cells/sample for processing and 10,000 cells/sample for sequencing) will be processed for single cell 5' RNA, V(D)J, and Feature Barcode sequencing utilizing the 10X Chromium system. Sequencing will be performed on the Illumina NovaSeq 6000 targeting 50,000 reads per cell for the 5 assay and 5,000 reads per cell for the V(D)J and Feature Barcode assays.