GSM8659007: heart, Old, 4OG; Mus musculus; RNA-Seq
Excess OCT was trimmed away from the frozen tissues block with a sterile scalpel blade. The tissues were cut into sections of 20 µM using a cryostat previously cleaned with a RNAse away solution. 10 sections per sample were collected in microtubes containing 1mL of Trizol. The RNA was isolated using the TRIZOL/Chloroform extraction method. RNA was quality controlled with Agilent Fragment Analyzer System. Library preparation was performed by Strand-specific cDNA library, purification of poly-A containing mRNA molecules, mRNA fragmentation, random primed cDNA synthesis (strand-specific), adapter ligation and adapter specific PCR amplification.