GSM8945625: Taproot, drought stress, transcriptome, biol rep 3; Quercus robur; RNA-Seq
Meristematic zones from both taproots and lateral roots were collected from all experimental groups (control and drought-stressed plants from both cultivation methods). Total RNA was extracted using Total RNA Purification Kits from Norgen Biotek, followed by additional purification with RNA Clean-Up and Concentration Kits. RNA quality was rigorously assessed through three complementary methods: concentration measurement via Nanodrop spectrophotometry, integrity evaluation by agarose gel electrophoresis, and precise quality scoring using an Agilent 2100 Bioanalyzer, with only samples achieving RNA Integrity Numbers (RIN) greater than 8 proceeding to library construction. High-quality RNA samples were processed into cDNA libraries using the TruSeq Stranded mRNA LT Sample Prep Kit, followed by sequencing on an Illumina NovaSeq platform configured for 150 bp paired-end reads for transcriptome, 50 bp single-end reads for miRNA and 50 bp single-end reads for degradome. The sequencing strategy encompassed comprehensive profiling of mRNA transcripts, miRNA molecules, and degradome fragments, with a minimum sequencing depth of 20 million reads per sample to ensure robust data acquisition. All library preparations and sequencing runs were performed in triplicate to guarantee experimental reproducibility.