GSM9144393: 24 hpf whole embryo pool (n = 50), sgshΔex5-6 homozygous, rep1; Danio rerio; RNA-Seq
Prior to total RNA extraction: - 24 hpf embryos were manually dechorionated in E3 medium, then transferred to Eppendorf tubes on ice. - 5 dpf larvae were euthanised by tricaine overdose, then immediately decapitated in ice-cold 1x PBS. Heads were collected into an Eppendorf tube on ice. - Adult zebrafish were euthanised in an ice-water slurry, then exsanguinated by tail resection on ice. Following exsanguination, whole brains were dissected from the neurocranium, rinsed in fresh ice-cold PBS, then transferred to an Eppendorf tube on ice.  Extraction method: Total RNA from all samples were harvested in TRIzol (Sigma) per the manufacturer's protocol. Libraries for 150 bp paired-end RNA-sequencing were prepared by BGI using the DNBSEQ method.