Seq of SF1_0h
In short, PCR was conducted with 341F and 806R primers to amplify the V3-V4 region of bacterial 16S rRNA genes. Then, the amplicon purification was carried out employing AMPure XP beads (Beckman Coulter, CA, USA). Library preparation for sequencing was carried out using the Nextera XT library prep kit (Illumina, San Diego, CA, USA).