GSM8579635: young replicate 3, H3K27Ac; Mus musculus; ChIP-Seq
After ChIP decrosslinking at 65C overnight with RNase A and 2-hours Proteinase K at 37C, DNA was isolated using phenol-chloroform isoamyl alcohol and ethanol precipitation. After DNA integrity was confirmed, the DNA fragments are repaired, dA-tailed.DNA fragments with A tail are ligated to sequencing adaptor followed by size selection and PCR amplification by Novagene