GSM9001171: ADNP ChIP in ADNP N-terminal mutant mESC, replicate 2; Mus musculus; ChIP-Seq
10^6 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 5% of chromatin was set aside as Input control. The chromatin was incubated with antibody and protein A/G coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ahel et al. 2025. Libraries were prepared using the NEB Next Ultra II DNA library prep kit according to manufacturers instructions.