GSM8864274: TeloHAEC 24hr Ctrl siRNA treated RNA-seq rep 1; Homo sapiens; RNA-Seq
Total RNA was extracted using a Qiagen Rneasy micro kit, according to manufacturer's recommendations. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification