Metagenomic sequencing of sub-surface sediment from 15m west of the outflow from the Rothera sewage treatment plant
DNA was extracted from sediment using the DNeasy Power Soil Pro Kit (Qiagen). Purified on AMPure XP beads. 400ng of DNA was used in the Rapid Barcoding Kit (Oxford Nanopore) and sequenced on a MinION flowcell R9.4.1