WGBS of Pan troglodytes: cultured cells
PBAT libraries for whole-genome DNA methylation sequencing were created from 100 ng of purified genomic DNA. Bisulfite conversion was done using the EZ DNA Methylation-Gold Kit. First strand synthesis used a biotinylated primer with a 90-minute incubation. Second strand synthesis employed a modified primer for TruSeq dual indexed sequencing, also with a 90-minute incubation. The final PCR amplification involved 8 cycles using standard Illumina TruSeq dual-indexed primers.