GSM9690417: run_0393a_A549, H460, SW1573; Homo sapiens; OTHER
Tumor were homogenized using gentleMACS dissociator (Miltenyi Biotec) following manufacturer protocol and gDNA extracted using Maxwell RSC 48 (Promega) with Maxwell® CSC Genomic DNA Kit sgRNA sequences were PCR amplified with primers forward: F: 5′- AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNNNNNNCTTGTGGAAAGGACGAAACACCG-3′, reverse: R: 5′-CAAGCAGAAGACGGCATACGAGATGTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3′ and purified with Kapa Pure Beads (Roche, KK8802)