GSM9112888: OVSAHO cells, 10 nM DHT Biol. Repl. 3; Homo sapiens; RNA-Seq
RNA was harvested using Macherey Nagel RNA extraction kit ((#740933.5, Macherey-Nagel GmbH&Co, Germany). mRNA sequencing was performed at Novogene Inc. mRNA was enriched using poly-T oligo-attached magnetic beads and fragmented. First-strand cDNA synthesis was performed using random hexamer primers, followed by second-strand synthesis using dUTP for directional libraries. Libraries were prepared by end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification. Library quality was assessed by Qubit, real-time PCR, and Bioanalyzer to determine concentration and fragment size distribution. Libraries were pooled and sequenced on an Illumina platform using paired-end 150 bp (PE150) reads, generating approximately 12 Gb of raw data per sample.