GSM8971825: H9, DMSO, replicate2; Homo sapiens; RNA-Seq
RNA was harvested using Rneasy mini plus kit (Qiagen). 50 ng of total RNA was used for the construction of sequencing libraries. For ChIP-seq, cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was digested using a Micrococcal Nuclease (CST). 10ug were used for library preparation. RNA libraries for RNA-seq were prepared using NEBNext Poly(A) mRNA Magnetic Isolation Module and xGen Broad-range RNA Library Prep following manufacturer's protocols. ChIP-seq libraries were prepared using NEBNext Ultra II FS DNA Library Prep Kit following manufacturer's protocol.