GSM8728306: DownstreamTAD2_48h, rep2; Homo sapiens; OTHER
Cells were fixed with 1% formaldehyde for 10 min at RT. The reaction was quenched with 0.25 M of glycine (final concentration) for 5 min at RT. Cells were then washed and crosslinked for the second time with ethylene glycol bis(succinimidyl succinate) EGS at a final concentration of 3 mM, the reaction was quenched with 0.4 M glycine (final concentration) for 5 min RT. Micro-C libraries were built and a tiled array of probes was used to enrich for the region of interest. Region Capture Micro-C libraries were prepared using NEBNext UltraII DNA Library Prep Kit for Illumina with NEBNext Multiplex Oligos for Illumina from New England Biolabs (NEB). After library amplification, DNA libraries were size selected at 100-1000 bp in length using SPRIselect size selection beads from Beckman Coulter.