RNA-seq of Mycolicibacterium sp. ELW1
Mycolicibacterium sp. ELW1 was grown on either fructose or isobutylene (IB) to early/mid log phase. NGS was performed by the NC State University Genomic Sciences Laboratory (GSL) (Raleigh, NC) by an Illumina NovaSeq 6000 using Block 150 PE 40M PE Reads. Sample libraries were prepared by the GSL for sequencing with Qiagen RNeasy Plus Micro Kit (Germantown, MD), NEBNext Bacterial rRNA Depletion Kit (Ipswich, MA), and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Ipswich, MA) all per the manufacturers protocol.