GSM8515775: S461591_WTA_023; Homo sapiens; OTHER
5μm thick FFPE slides were cut using a microtome. Deparaffin Protocol - Xylene 5min x3, 100% EtOH 5min x2, 95% EtOH 5min x1. 99°C ER2 (Ph9) 15mins. 37°C 1μg/mL Proteinase K 15mins. 10%NBF 5min, NBF Stop buffer 5min x2. 37°C. Label Protocol - WTA probe mix 1:10 16hr. 37°C 50%Formamide-2xSSC mix 25mins x2. Hybridisation Protocol - Syto13, CD45  TxRed, CD4 647 60mins. Scan Protocol - FITC - SYTO1350ms, TxRed - CD45 200ms, Cy5 - CD4 200ms Spatial transcriptome sequencing libraries were prepped with Nanostring Reagent kits according to manufacturer's suggestions.