GSM9543869: FLU_CD8_RECALL, GEX; Mus musculus; RNA-Seq
For bulk RNA-seq, total RNA was extracted from lung tissues using the Direct-zol RNA Miniprep Plus Kit. For scRNA-seq, single-cell suspensions from lung tissues were stained with NP366 tetramer and sorted using a FACSAria III with >95% purity. For bulk RNA-seq, mRNA libraries were prepared using poly-A enrichment and strand-specific protocols. For scRNA-seq, sorted cells were loaded into the Chromium Controller using Chromium Next GEM Single Cell 3' Reagent Kits v3.1.