=_xlfn.CONCAT("metagenome of soil sample ", B7)
Soil DNA was extracted at least in triplicate (0.25 g DW; some samples required more extractions to achieve the minimum amount of DNA required for sequencing) using the Power Soil DNA Isolation Kit (MoBio Laboratories Inc., Carlsbad, CA). DNA quality and concentration were determined using a TapeStationTM (Agilent Technologies Inc., Santa Clara, CA). Shotgun metagenomic sequencing, including library preparation, was performed at the Centro Nacional de Anlisis Genmico (CNAG-CRG, Spain).