Ribo-seq of  Eif6 WT  and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)
Ribo-seq of  Eif6 WT  and Eif6 S235A Mouse Embryonic Fibroblasts (MEFs)
Immortalized MEF cells were treated with 100ug/ml cyclohexamide for 2 minutes then washed once with ice-cold PBS 10ug/ml cyclohexamide.  Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cyclohexamide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Immagina Biotechnology LACEseq #LS-001_12