human gut microbiome
16S amplicon library preparation and sequencing were conducted following the Earth Microbiome Protocol (https://earthmicrobiome.org/). PCR amplification of the extracted DNA was performed for 30 cycles targeting the V4 region of the 16S rRNA gene. Universal primers 515F and 806R (515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT) were used with a Golay barcode on the forward primer.