GSM9317566: ryp1 lo MOI 48h 2; Coccidioides posadasii; RNA-Seq
RNA was extracted using the Direct-zol RNA Miniprep Plus isolation kit (Zymo). Libraries were made with the NEBNext polyA mRNA magnetic isolation module and NEBNext Ultra II Directional RNA Library Prep kit with dual-indexed multiplexing barcodes as instructed.  Libraries were pooled and sequencing was performed by the Chan-Zuckerberg BioHub-San Francisco on one lane of Novaseq S4.