eDNA metabarcoding of sediment and water samples: Ave4 (CRX1913294; CRA028973)
Total eDNA was extracted using the DNeasy Blood and Tissue Kit and the DNeasy PowerMax Soil Kit, a primer set 12Sa/12Sh was used to amplify a fragment of the 12S mitochondrial rDNA, libraries were constructed using a PCR free protocol and sequenced on an Illumina NovaSeq 6000 platform with 150 bp paired end reads.