GSM9176893: Atf7ip KO; Mus musculus; RNA-Seq
For scRNA-seq, TCRb+CD3+ T cells from three to four mice of each genotype were pooled and FACS sorted into 1.5 ml Eppendorf tubes containing 500 ul of DMEM with 2% FBS. Approximately 20,000 cells per 100 ul were sent to the UCSF Genomics Core for processing. Single cells were captured using the 10X Chromium microfluidics system (10x Genomics). The cells were then barcoded complementary DNA libraries and sequenced on the NovaSeq (Illumina).