GSM9111089: iNeuron, Scramble shRNA, rep 1; Homo sapiens; RNA-Seq
Total RNA was isolated using RNeasy mini kit (Qiagen) combined with DNase I treatment according to the manufacturer's protocol. Total RNA from cells treated with scrambled shRNA or SMN1/2-targeting shRNA was used to construct RNA-sequencing libraries using a ribosomal RNA depletion protocol (RiboErase), performed by the Stanford Genomics Core Facility. The resulting libraries were quantified, pooled, and sequenced on an Illumina NovaSeq 6000 using an S2 flow cell with 150 bp paired-end reads.