GSM8516358: HA_NSC_CUTandTag; Drosophila melanogaster; OTHER
For CUT&Tag carried out by using neural stem cells, the cells were arrested by ConA beads, and the HA tag fused factor of interest are combined by the specific primary antibody (HA), followed by secondary antibody and Tn5 transposase. CUT&Tag libraries were prepared using Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme) complemented with TruePrep Index Kit V2 for Illumina (Vazyme). 1w-100w cells were used as starting materials. Libraries were amplified using 13-19 cycles on the thermocycler. Post-amplification libraries were size selected at 200-2000 bp in length using VAHTS DNA Clean Beads (Vazyme).