GSM9317353: KO1; Mus musculus; OTHER
CUT&Tag was performed following the protocol of the Hyperactive™ In-Situ ChIP Library Prep Kit for Illumina (Vazyme, TD903-TD904). Briefly, cells were bound to concanavalin A–coated magnetic beads and permeabilized with digitonin. Bead-bound cells were sequentially incubated with primary antibody (H3K36me2), secondary antibody, and Hyperactive pA-Tn5 transposase for targeted chromatin tagmentation. Tagmented chromatin fragments containing inserted P5 and P7 adapters were PCR-amplified using index primers. The amplified libraries were purified and quality-checked using an Agilent 2100 Bioanalyzer. Final libraries were sequenced on an Illumina NovaSeq 6000 platform to generate 150-bp paired-end reads.