GSM8516522: 22Rv-1 cells,lv-shcontrol 2; Homo sapiens; RNA-Seq
Cells were plated in 10-cm dishes at a density of 1.5 × 106 cells/dish in triplicate and incubated overnight for attachment. They were lysed with TRIzol Reagent according to the manufacturers' instructions. The lysates were sent to Personalbio (Shanghai, China) for cDNA library construction and sequencing by Illumina NovaSeq 6000 The lysates were sent to Personalbio (Shanghai, China) for cDNA library construction and sequencing by Illumina NovaSeq 6000. RNA-sequencing reads were first trimmed to remove poly(A) and unqualified reads with Cutadapt(v2.7).