GSM8946527: Endocervix, Fibroblasts, E, E2, chip, rep3; Homo sapiens; RNA-Seq
Cells were harvested from apical and basal channels of the Cervix chips using Buffer RLT plus (Qiagen, 1053393). Lysates were stored at –80 °C prior to processing. Total RNA was submitted to Genewiz (Azenta Life Sciences) for next generation sequencing (Standard RNA-seq, polyA selection with ERCC spike-in, sequencing using an Illumina HiSeq for 2x150bp, ~350M PE reads, single index).