WGS of Saccharomyces cerevisiae  a low-ethanol-producing strain  isolated from the lees of huangjiu
For Illumina pair-end sequencing, at least 3g genomic DNA was used for sequencing library construction for each sample. Paired-end libraries with insert sizes of ~450bp were prepared following Illuminas standard genomic DNA library preparation procedure. Purified genomic DNAis sheared into smaller fragments with a desired size by Covaris, and T4 DNA polymerase was applied to generate blunt ends. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate followed by a library quality test. At last, the quantified Illumina pair-end libraries sequenced by Illumina NovaSeq 6000 platform (150bp*2, Shanghai Biozeron Biotechnology Co., Ltd, Shanghai, China).