GSM8971684: Aba_sigAb_ChIP_HaloTag3; Acinetobacter baumannii; ChIP-Seq
Cultures were crosslinked with formaldehyde, quenched with glycine, and 50 mL cell pellets were harvested. Pellets were sonicated in a Covaris Misonix sonicator for 16 min (20% duty factor, 75 PIP, 200 cycles per burst, 6°C) to achieve ~100-500 bp fragments and immunoprecipitation of HaloTagged-SigAb protein was performed according to Promega HaloChIP protocol. Before IP, 1/10 of each sample was saved as input control. Input control and HT-enriched samples were prepared for Illumina sequencing using NEBNext Ultra II Library Prep Kit for Illumina (NEB; E7645S) following manufacturer's protocol.