GSM8970285: KO biol rep 5 untreated; Mus musculus; RNA-Seq
RNA preparation was conducted using the RNeasy Micro Kit (Qiagen). RNA quantity was determined with a Qubit 2.0 fluorometer (Invitrogen), and RNA quality  was assessed on a Bioanalyzer 2100 (Agilent) using an RNA Pico Chip (Agilent) to determine the RNA integrity number (RIN value). Samples with a RIN value  of >8 were used for library preparation. Samples were then submitted to Novogene (Cambridge, UK) for high-throughput RNA sequencing. Samples with a RIN value of >8 were used for library preparation and sequencing (approx. 30mio reads/sample; PE150) at Novogene (Cambridge, UK).