GSM8832648: hiPSC-cardiomyocyte, icell, sham, day 7, biol rep 2; Homo sapiens; RNA-Seq
Cell preparation, antibody binding, tagmentation, and DNA extraction and purification were performed using the Active Motif CUT&Tag kit (Active Motif, 53171) according to the manufacturer's instructions. The following antibodies were used: H3K27me3 (Active Motif, 39155), H3K27ac (Active Motif, 39685), H3K4me3 (Active Motif, 39159), H3K4me1 (Cell Signaling Technology, 5326), or IgG isotype control (Cell Signaling Technology, 66362S). cDNA libraries were constructed using the Active Motif CUT&Tag kit with Nextera primers per manufacturer's instructions.