GSM8945611: RIP_tas3a/b/c_Rep1; Arabidopsis thaliana; RIP-Seq
Total RNA was extracted from 12-day-old Arabidopsis seedlings using the TRIzol method (Takara, 9108). WT and mutant seedlings underwent treatment with cell lysis buffer (1M Tris-HCl, 5M NaCl, 10% NP-40, 1M MgCl2, 1M DTT, proteinase inhibitor cocktail (100x), RNase inhibitor) to extract total protein. Subsequently, AGO1 antibody (Agrisera, #AS09527) and Protein A dynabeads (Thermo, 10001D) were utilized to enrich AGO1 protein. Following this enrichment process, RNA extraction and construction of the small RNA (sRNA) sequencing library were conducted as previously described.