GSM8658292: CDK12 WT, metastatic prostate adenocarcinoma - 1; Homo sapiens; OTHER
Spatial transcriptomics was performed using the Visium HD, Human Transcriptome (10x Genomics; #1000673) and Visium CytAssist (10x Genomics; #1000441) following the instructions from the manufacturer. Briefly, 5 µm FFPE tissue on tissue slide was applied to HE staining, imaging, destaining, and decrosslinking. Tissue was nexted permeabilized and hybridized with human transcriptomic probes overnight. Hybridized probes were then transferred to the Visium slide, which contains millions of barcodes, through the CytAssist instrument. cDNAs were synthesized and amplified with sample indexes for the final libraries. Quality of the libraries was evaluated by Agilent Bioanalyzer.