GSM8658674: ChAT-GFP negative liver B cells, 4 hours post-PHX, rep 4; Mus musculus; RNA-Seq
ChAT-GFP reporter mice were subjected to 70% PHX or Sham surgery and liver non-parenchymal cells were enriched as described above. B cells were stained and sorted on a BD FACSAria Fusion flow cytometry cell sorter at the Princess Margaret Flow Facility. RNA was extracted using the RNAeasy micro kit (QIAGEN) based on the manufacturer's instructions. Sample library preparation was performed using a Mammalian Low Input V2 Pico kit (Takara) based on the manufacturer's instructions.