GSM8727520: HEK293T_random_cyto_2; Homo sapiens; RNA-Seq
For P-body-seq, RNA was isolated from P-body and cytoplasmic fractions using the miRNeasy Micro Kit (QIAGEN). For polyA RNA samples, cDNA libraries were constructed using the V4 ultra + Nextera XT kit for Illumina (Takara Bio). For P-body-seq for total RNA samples, libraries were prepared using Snap seq protocol. Libraries were sequenced with paired-end 150-bp reads.