GSM8864961: MCF-7 LSKAA replicate 2; Homo sapiens; ChIP-Seq
Cells were grown in 150 mm2 plates, crosslinked for 10' in 2% PFA and lysed. Cells were sonicated in 10 cycles of 30'' ON/OFF using Bioruptor Pico Tubes (Diagenode) with sonication beads (Diagenode) in a Bioruptor sonicator (Diagenode). Sonicated chromatin was incubated with anti-HA antibody (ab9110, Abcam) at 4ºC in rotation o/n. Chromatin-antibody complexes were pulled down using Protein G dynabeads (ThermoFisher, 10003D) and magnetic rack. Isolated chromatin was decrosslinked at 65ºC for 4 h and DNA was purified with Qiagen DNA purification kit (Qiagen, 56304). Repaired dA-tailing