GSM8944524: FL-KCTD10 HeLa, DMSO, rep1, FLAG(CUT&RUN); Homo sapiens; OTHER
CUT&RUN was performed as previously described (Skene PJ and Henikoff. Elife. 2017). Cells were incubated with ConA magnetic beads for 10 min at room temperature. Samples were incubated in primary antibody overnight at 4 C with rotation. Samples incubated  with pA/G-MNase for 1 h at 4 C and then digested on ice for 1 h. Solubilized chromatin was released and DNA was extracted with phenol-chloroform isoamyl alcohol and ethanol precipitation. CUT&RUN libraries were prepared by NEBNext Ultra II DNA Library Prep Kit for Illumina.