GSM9396635: CAF2_CON1; Homo sapiens; RNA-Seq
Total RNA was extracted from all samples using TRIzol reagent (Thermo Fisher Scientific, USA) following the manufacturer's instructions. RNA concentration and purity were measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, USA), and RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Library preparation is performed using Optimal Dual-mode mRNA Library Prep Kit (BGI-Shenzhen, China).