Repli-MiC-K562_TOP1-depleted_RepliMiC
Libraries were constructed by the Repli-MiC strategy. Briefly,the TOP-FKBP K562 cells (1G4 clone) were treated with dTAG for 6 hours and BrdU for 15 min following fixed with 1% formaldehyde, digested by MNase,END-blunting, dA-tailing, and ligated with biotinylated linkers. Fragmentated DNA was enriched by anti-BrdU and then biotin. After that, DNA was tagged with adapters and sequenced.