E0
Library sequencing was performed using the Targeted RNA Library Preparation kits Illumina (NEB, USA), NEBNextUltra and Illumina Novaseq 6000. Cleavage was performed in NEBNext First Strand Synthesis Reaction Buffer (5X) using second-valent cations at elevated temperatures. Phusion high fidelity DNA polymerase, PCR primers and Index (X) primers were used for PCR. finally, Agilent Bioanalyzer 2100 system (AMPure XP system) was used for purification of the products and evaluation of the library quality.