GSM9113222: Ctrl_P12_1; Homo sapiens; RNA-Seq
RNA was extracted using Trizol following the manufacturer's protocol. DTT, dNTP, RNase Inhibitor, RT Enzyme and 5x RT Buffer are added to the previous product to synthesize a strand cDNA.  The supernatant was removed by magnetic separation, and single-stranded DNA was removed by adding Exonuclease I and 10x Exonuclease I Buffer. The supernatant was removed by magnetic separation, and the Poly(A) RNA was removed by adding Elution Buffer. The supernatant was removed by magnetic separation. Random primer, 10x Reaction Buffer, enhancer, dNTP and cDNA Extend Polymerase I were used for the two-strand cDNA synthesis.  After removed the supernatant, Elution Buffer was added. Subsequently, DNA beads were added to the supernatant for the fragments recovery. Then 2x HIFI Amplification Mix and index were added and performed PCR Amplification for library fragments. DNA beads were screened for library fragments.