Illumina NovaSeq 6000 paired end sequencing of SAMD01819862
"Sample: Cell Barcode, B6-90-1m-1dpi-01: GCTAACTGT, B6-90-1m-1dpi-02: TAATCAGTC, B6-90-1m-1dpi-03: TAATCTAGC. BRB-seq(Alpern D et al.) was performed for preparing libraries with some following modifications. Oligo-dT based primer was used for single-stranded synthesis and Second Strand Synthesis Module (NEB, #E6111) was used for double-stranded cDNA synthesis. In-house MEDS-B Tn5 transposase (Picelli S et al., Sato et al.) was used for tagmentation and amplified by 10 cycles of PCR using Phusion High-Fidelity DNA Polymerase(Thermo Scientific, #M0530). 81bp of insert read (Read2) were obtained on Illumina NovaSeq6000."