GSM8607146: BT286 DMSO 1; Homo sapiens; RNA-Seq
RNA was isolated using the RNeasy Mini Kit with an on-column DNase digestion (79254, Qiagen). Resulting RNA concentrations were quantified with a Nanodrop 2000 Spectrophotometer (ND-2000, Thermo Scientific), and up to 500 ng were submitted for total RNA sequencing. Samples were subjected to rRNA depletion using the QIAseq FastSelect -rRNA HMR kit (334385, Qiagen) and sequencing was performed to generate 50M 150 bp read pairs (100M reads) per sample.