gene expression of CK3 (CRX1829823; CRA027562)
Total RNA was extracted from honeybee (Apis mellifera) midgut tissues of control and miticide mixture (MM)-treated groups. RNA quality was assessed using an Agilent Bioanalyzer. Sequencing libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit, following the manufacturer's protocol. Libraries were sequenced on an Illumina platform to generate paired-end reads. The experimental design included biological replicates for each group to ensure robust differential gene expression analysis.