Drug Resistance Amplicon sequencing of Pf3D7: TBKA-KAS-040-2021-MSMT-1
Dried blood spots were collected from patients in health facilities. Blood spots were amplified with molecular inversion probes (MIPs) corresponding to known drug resistance loci. PCR was used to amplify and linearize successful MIP capture reactions and to add sequencing barcodes. Data was demultplexed and analyzed using the MIPTools software package (www.github.com/bailey-lab/MIPTools)