GSM8945658: mRNA_WT_tas2batch_Rep2; Arabidopsis thaliana; RNA-Seq
Total RNA was extracted from 12-day-old Arabidopsis seedlings using the TRIzol method (Takara, 9108). Initially, polyA-tailed RNAs were selectively enriched from the total RNA pool using oligo d(T) beads. These enriched RNAs were subsequently fragmented into random segments. Utilizing these segments as templates and dNTPs as building blocks, double-stranded cDNA was synthesized. Following purification, end repair, and adapter ligation, cDNA segments measuring 370-420bp were selected for amplification to create the RNA sequencing library.