16S rRNA amplicon sequencing of chicken cecum microbiome sample C3
Genomic DNA was extracted from chicken cecum samples and subjected to PCR amplification targeting the bacterial 16S rRNA gene. Amplicons were purified by gel electrophoresis, followed by end repair, A-tailing, and ligation of Illumina sequencing adapters. Adapter-ligated fragments were PCR amplified, size-selected, and purified to construct sequencing libraries. Library quality and fragment size distribution were assessed using AATI, and library concentration was quantified by qPCR. Qualified libraries were pooled and sequenced on an Illumina platform using paired-end sequencing.