GSM9032553: R255X-MUT_hCO_bulk RNA-seq_2; Homo sapiens; RNA-Seq
Total RNA was extracted from pooled organoids using TRIzol Reagent (Invitrogen, 15596026-100ML), and up to 5 µg of RNA was subsequently treated with DNaseI (Roche, 04716728001), according to the manufacturer's instructions. RNA was stored at - 80 ºC until further use. Bulk RNA-seq libraries were prepared using the Illumina Stranded Total RNA kit and pair-end sequenced on a single lane of a NovaSeq X Plus (Illumina) flow cell with a 10% PhiX target.