GSM9081384: CIH_mm10_vM23; Mus musculus; RNA-Seq
Immediately after dissection, the lungs were pooled (3 mice for each group) and then minced mechanically before dissociation with a protease mix, filtered, washed, and counted for viability. Library was performed according to the manufacturer's instructions, (Chromium Single Cell 3' v3.1 kit.). Briefly, single-cell suspensions were loaded onto 10x Genomics Chromium Next GEM Chip G and partitioned into droplets with barcoded gel beads using the Chromium Controller. Reverse transcription tagged mRNAs with cell barcodes and UMIs. cDNA was amplified, fragmented, and converted into sequencing libraries using the Chromium Single Cell 3' v3.1 kit. Libraries were sequenced paired-end on Illumina NovaSeq 2000 (28 bp R1, 8 bp i7, 91 bp R2).