GSM8516481: root; polysome; replicate 1; Arabidopsis; OTHER
Total RNA isolation: three volume of LS trizol was added to the samples and mixed well for the RNA extract. The RNA was run in 15% 10 Well TBU gel (EC6885BOX) to select RNA fragment on the size between 20 nt to 40 nt (Pre-stained ladder (DM253S) was used for size reference). Then, libraries were constructed following the procedures of NEB E7330S kit. The constructed libraries were sequenced on the Illumina HiSeq sequencing platform in single read mode with 100 bp per read.