GSM9064999: Vgll3 Ex3 Sample 1B; Nothobranchius furzeri; RNA-Seq
Fibroblast cultures in a 10 cm plate at 90% confluency were harvested by incubating them for 5 min in ice-cold PBS, and then adding  1 mL of TRI Reagent. RNA purification was performed using Direct-zol RNA Miniprep Kit (Zymo Research # R2052) according to the manufacturer's instructions. RNA concentration and quality were determined by using an Agilent 2100 bioanalyzer (Agilent Technologies). Library preparation was performed using KAPA mRNA HyperPrep Kit (ROCHE-08105936001) according to the recommended protocols. Library quantity and pooling were measured by Qubit (dsDNA HS, Q32854), size selection at 4% agarose gel. Library quality was measured by Tape Station (HS, 5067-5584). Libraries were sequenced by NextSeq 2000 P2, 100 cycle,75 bp single end (Illumina, 20024906) with  ~30 million reads per sample for vgll3.