GSM8694060: rs1173771_alt_highBP_iVSMC cells_region capture Micro-C; Homo sapiens; Hi-C
Using Dovetail Micro-C Kit following the manufacturer's User Guide. Dovetail Micro-C kit was used following manufacturer's User Guide to create Micro-C libraries. Region capture Micro-C libraries were then generated with reagents from Dovetail targeted enrichment panels. Indexed Micro-C libraries were pooled together to perform region capture Micro-C. The probe panel targeting human chr5: 32,443,700-33,470,100 (hg38) was designed and synthesized by Twist Bioscience. The panel of 80bp probes covers the rs1173771 LD region and promoters of adjacent protein coding genes. The libraries were sequenced using the Illumina NovaSeq sequencers.