GSM8516287: cKO; Mus musculus; RNA-Seq
Fresh tumor samples obtained from groups of MTCH2fl/fl and MTCH2cKO mice were pooled, rinsed with PBS, minced, and dissociated with 1× Trypsin-EDTA solution for 20 minutes at 37°C. A Countstar Fluorescence Cell Analyzer was used to assess the cell viability of dissociated single cells. The cDNA synthesis and library construction for scRNA-seq strictly adhered to the protocols of the respective manufacturers. Briefly, unique RNAs released from individual cells underwent barcoding by reverse transcription in individual gel bead-in emulsions. This process yielded cDNA, which was then amplified and quality-checked to ensure accuracy. Subsequently, the libraries were sequenced on an Illumina Novaseq6000 platform, employing a pair-end 150-bp strategy that achieved a minimum sequencing depth of 100,000 reads per cell to guarantee comprehensive coverage and analysis, by CapitalBio Technology.