GSM8658133: Ctrl_3; Homo sapiens; RNA-Seq
Total RNA was extracted from the samples using Trizol reagent (Yeasan,China) as described in previous studies. These RNA samples were further purified using chloroform and isopropanol and then treated with RQ1 DNase (Promega) to remove DNA. Utilizing a Smartspec Plus (BioRad) instrument, the absorbance at 260/280 nm (A260/A280) was measured in order to assess the purity and quantity of the RNA. Following the manufacturer's instructions,cDNA libraries were prepared for high-throughput sequencing and applied to the Illumina Novaseq 6000 system for 150 nt paired-end sequencing.