GSM8516796: DKOwBF_3; Danio rerio; RNA-Seq
RNA extraction, DNase treatment was done using Zymo Quick-RNA Microprep Kit. RNA later was checked integrity and concentration by D1000 Agilent ScreenTape. Samples with RIN values ranged from 7.2-9.3 were further librarized with 30ng of RNA as starting point. RNA library was prepared using NebNext Ultra II kit following manufacturer's protocol