GSM9401864: Endothelial enriched PyMT-M; Mus musculus; RNA-Seq
Mammary tumors were harvested from female C57BL/6 mice, minced, and enzymatically digested with 1.5 mM collagenase I and 1 μg/mL DNase I at 37°C for 1 hour with rotation. Digested tissue was passed through a 70 μm strainer, washed with PBS containing 2% FBS, and live cells were subjected to endothelial magnetic enrichment and subsequently isolated by sorting prior to pooling, single-cell library preparation and downstream analysis. Single-cell suspensions were processed using the 10x Genomics Chromium Single Cell 3′ Gene Expression platform (v3.1 chemistry) according to the manufacturer's instructions. Libraries were prepared from sorted live cells, amplified, and sequenced on Illumina X platform at BCM RNA Profiling Core.