Genome sequencing of Rhodopirellula sp. UH5, Nanopore reads
Genomic DNA was extracted with the Wizard HMW DNA Extraction Kit (Promega) following the manufacturers instructions, but instead of 100 microliters rehydration buffer, 50 microliters TE buffer (10 mM Tris-HCl, pH 8.0, 1 mM EDTA-Na2, Promega) were used. Materials und tools used: ONT Sequencing Kit: SQK-NBD114.24, ONT Flow cell: FLO-MIN114, Bascaller: Dorado 1.1.1, Basecalling_model: dna_r10.4.1_e8.2_400bps_sup@v5.2.0, Demultiplexing: Dorado 1.1.1, Q10 filtering: Dorado 1.1.1, Read QC long reads: NanoPlot 1.46.1, Assembly: Flye 2.9.6 (--nano-hq), Long-read Polishing: Medaka 1.7.2, Medaka Model: r1041_e82_400bps_sup_g615