metatranscriptomic sequencing of Rn's gut in SZ-Rn-03 (CRX1819695; CRA027291)
After removing host rRNA using the Ribo-off rRNA Depletion Kit (Human/Mouse/Rat) (Vazyme, China), the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, China) was used for library preparation. Each RNA library was then sequenced on the Illumina HiSeq 2000/2500 platform (Genewiz Biotechnology Co., Ltd., China) with 195 bp paired-end reads .