GSM8658208: human A549 cells, PR8/H1N1 influenza virus infected, 24 hpi; Homo sapiens; RNA-Seq
1 mL Tri-reagent (Sigma) was added to each sample, and RNA was harvested according to the manufacturer's instructions. The ribosomal RNA is removed and linear RNA was digested by RNase treatment.  RNA was reverse transcribed using random primers and then was detected by  RNA-seq. RNA libraries were prepared for sequencing using standard  TruSeq DNA PCR-Free Library Preparation Kit  protocols.