RNA-seq of T. californicus: BB control 2
RNA was extracted from pools of adult male copepods from Bodega Bay and La Jolla that underwent modified acute exposure experiments in which they were exposed to either 60 mg/L Cu or 35C for 24 hours. Pool size was 24 animals for the control and heat treatments and 36 animals for the copper treatment. Immediately after the conclusion of the exposure period animals were transferred into ZR BashingBead Lysis Tubes (Zymo Research) containing TRI reagent (ThermoFisher Scientific) and homogenized using a TissueLyser II (Qiagen). Extractions were then performed with a Direct-zol RNA Miniprep kit (Zymo Research) according to the manufacturers instructions. The RNA concentration in each sample was quantified using an Invitrogen Quibit 4 Fluorometer (ThermoFisher Scientifc) and a Quibit RNA High Sensitivity Assay Kit (ThermoFisher Scientific). Libraries were prepared using a NEB Next Ultra II Directional Library Prep Kit (New England Biolabs) and sequenced in 150 bp paired-end reads on an Illumina platform at Admera Health (New Jersey, USA).