GSM9032491: wt; Danio rerio; RNA-Seq
ACME (ACetic-MEthanol), a dissociation protocol for single-cell transcriptomics, which simultaneously fixes the tissue, was used to avoid potential effects of the dissociated tissue architecture to gene expression (García-Castro et al., 2021). Briefly, shield-stage wildtype and MZwnt11/slb embryos were dechorionated and treated with the ACME solution. Dissociated cells were cryopreserved by adding 10% DMSO and stored at −80 °C. cDNA libraries targeting ~10,000 cells per sample were prepared using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Genomics) following the manufacturer's instructions.