GSM9543815: P14 hippocampus H3K27ac ChIP-seq Sal rep3; Mus musculus; ChIP-Seq
Hippocampal tissue (~60 mg) was fixed in 1% formaldehyde at 4C for 10 min, quenched with 125 mM glycine, lysed and sonicated on a Bioruptor Pico for 30 cycles (30 s ON/30 s OFF) to 200-500 bp. Chromatin (50 ug) was immunoprecipitated overnight with 5 ug anti-H3K27ac (Cell Signaling) and Dynabeads Protein A. After washes, crosslinks were reversed at 65C for 4 h and DNA was purified. Libraries were built with the NEBNext Ultra II DNA Kit (10 PCR cycles) and sequenced as single-end reads on a NovaSeq 6000.