genomen of phage
(1) DNA fragmentation: The DNA fragment was produced by the M220 Focused-ultrasonicator(Covaris, USA) to a size of 300-500bp; (2) Library construction: Libraries for sequencing wasconstructed by using the TruSeg DNA Sample Prep Kit (lllumina, USA), and remove librariesoutside the range of 400-600bp; (3) The librarysequenced using lumina paired-endsequencing on the MiSeg platform as a part of a flowcell (2 250 cycles; llumina), and the readsquality controlled and error corrected were as followed: Filter out the adapter sequence fromreads, remove the non-A, G, C, and T bases at the 5 'end, filter out reads with N ratio greaterthan 10%, filter out reads with base (0>=20) account for less than 40%, filter out the reads withsize less than 75bp, (4) Genome assembly: the effective reads are assembled into contiasunder the linux environment through overlap, and contigs are cyclically filled by SPAdesGenome Assembler version 3.1.0 until the complete scalfords are generated. The onlineBLASTp server was used to verify the predicted open reading frames (ORFs).