GSM9212520: WT_CT_E18O_S5P-PolII_rep1; Mus musculus; OTHER
FACS-sorted cells were isolated from ovaries and pooled as one replicate. CUT&Tag libraries of oocytes were generated according to the protocol (a step-by-step protocol https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3) using CUTANA™ pAG-Tn5 (Epicypher, 15-1017). To perform quantitative spike-in CUT&Tag, Drosophila S2 cells were added to mouse oocytes at a fixed ratio (e.g. 5,000 S2 cells to 10,000 mouse oocytes) at the beginning of each reaction. CUT&Tag libraries were prepared using NEBNext HiFi 2x PCR Master mix (NEB, M0541L) according to the protocol (a step-by-step protocol https://www.protocols.io/view/bench-top-cut-amp-tag-kqdg34qdpl25/v3 ).