GSM8577742: MCD_T_2; Mus musculus; RNA-Seq
RNA was extracted and its integrity and quantity were assessed using the Agilent 2100 bioanalyzer. For library construction, total RNA was enriched for mRNA with poly-A tails using Oligo(dT) beads, fragmented, and used to synthesize cDNA, which was purified, end-repaired, A-tailed, and ligated with sequencing adapters. cDNA fragments of 370-420 bp were selected, PCR amplified, and purified to obtain the final library, which was quantified, diluted, and checked for insert size and concentration.