GSM9322711: WT, rapamycin, 2 hours, rep 1; Saccharomyces cerevisiae; RNA-Seq
Total RNAs were extracted from frozen cell pellets using hot phenol-chloroform extraction followed by ethanol precipitation. RNA libraries were prepared from 1 ug of total RNAs using the Direct-RNA sequencing kit from Oxford Nanopore (ONT, catalog #: SQK-RNA004) as per the manufacturer's instructions.