GSM8863786: HUDEP-2_EnhKO_CBS_CPC_rep2; Homo sapiens; OTHER
After ligation, DNA was subjected to proteinase K and pronase digestion, followed by purification using phenol:chloroform:isoamyl alcohol (25:24:1, v/v). Enriched and amplified DNA was then purified using a custom-prepared SPRIselect bead solution, optimized for 3–4 kb DNA by modifying the stock buffer. After two rounds of enrichment, DNA was processed using the Ligation Sequencing Kit V14 (Oxford Nanopore Technologies, SQK-LSK114). Finally, 200 fmol of DNA libraries were loaded onto a pre-rinsed PromethION flow cell (Oxford Nanopore Technologies, FLO-PRO114M).