GSM9317643: G793A, PLX3397, rep1; Mus musculus; RNA-Seq
RNA was harvested using Rneasy mini kit (Qiagen). RNA libraries were made using the Illumina TruSeq Stranded mRNA kit which utilizes oligo-dT beads to select and amplify mRNA. cDNA was synthesized using nine rounds of PCR and quality and concentrations of the DNA libraries were assayed using the Agilent 2100 Bioanalyzer high sensitivity DNA assay and the DNA high sensitivity Qubit.The libraries were quantified by Kapa qPCR, normalized to 1.5nM and then multiplexed at equimolar concentrations for sequencing on the Illumina NovaSeq 6000 platform.