GSM8970092: HD3, DIF-WT, CTCT, rep2; Gallus gallus; ChIP-Seq
Cells were crosslinked with 1% paraformaldehyde for 10 minutes and quenched with 0.125M Glycine. Nuclei were extracted and lysed, and chromatin was sonicated in a Covaris M220 sonicator to an average of 300 bp fragments. Immunoprecipitation was performed overnight with the CTCF antibody at 4°C. Immunoprecipitated chromatin was the incubated with magnetic beads, washed, TAGmented with Tn5 transposase and eluted from beads. Then, chromatin was decrosslinked and DNA was purified using MinElute kit (Qiagen) Preparation of ChIPmentation libraries was performed as described for ATAC-seq assays in Buenrostro et al., (2015)