WGS of Klebsiella pneumoniae isolate KPNW
A liquid culture of the isolate was subjected to DNA extraction and purification by using Agencourt AMPure XP Kit. Adapter ligation and library preparation of the DNA fragments was done by the Ion Xpress Plus Fragment Library kit and the Ion Xpress Barcode Adapters Kit. Upon purification of the adapter-ligated and nick-repaired DNA by Agencourt AMPure XP Reagent and size selection of Library with the E Gel SizeSelect II Agarose Gel, whole genome sequencing was performed on an Ion GeneStudio S5 Series System, which is a semiconductor-based NGS platform.