GSM8606653: Control Rep3; Mus musculus; RNA-Seq
Tumors were collected from mice treated with control IgG, anti-PD1 antibody alone, anti-CXCR4 antibody alone, and anti-PD1 antibody in combination with anti-CXCR4 antibody. Total RNA was extracted from the freshly isolated tumor tissues using Qiagen RNeasy Mini Kit (Cat. 74104). Libraries were prepared using Roche Kapa mRNA HyperPrep strand specific sample preparation kits from [200ng] of purified total RNA according to the manufacturer's protocol on a Beckman Coulter Biomek i7.