GSM9141065: MOLM13, 1 µM A485, 12h, rep2; Homo sapiens; OTHER
Harvested cells were washed with PBS, nuclei were isolated using a Dounce homogenizer. RNA was extracted after run-on reaction and chemically modified. The modified RNA was enriched, reverse transcribed and libraries were PCR amplified with 17 cycles on a thermocycler. The resulting library was purified with Ampure XP beads from Beckman Coulter.