GSM8517159: Gad2-cre, GFP, RNA, Group B, sample2; Mus musculus; RNA-Seq
Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's procedure. The cleaved RNA fragments were reverse-transcribed to create the cDNA by VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, China), which were next used to synthesise U-labeled second-stranded DNAs with VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme, China). The average insert size for the final cDNA library was 300±50 bp.