GSM9032621: WT_ORX_R3; Mus musculus; RNA-Seq
Total RNA was extracted from ~ 50 mg of mouse gastrocnemius (androgen deprivation experiment) or spinalis (DHT treatment experiment) by phenol chloroform extraction (1 mL TRIsure solution; Bioline), purified using the RNeasy Mini Kit (Qiagen) and RNA integrity was determined using TapeStation (Agilent Technologies 2200). Libraries were prepared using the Illumina TruSeq Stranded Total RNA GOLD.