F30D (CRX1779000; CRA026836)
To obtain global transcriptome data, high-throughput mRNA sequencing analysis was performed using the Illumina sequencing platform. The quality of the total RNA samples was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, U.S.A). RNA samples with an RIN value of > 6 were used for library construction. The transcriptome library was constructed using the VAHTS Universal V5 RNA-seq Library Prep kit according to the instructions, and the library was sequenced using the Illumina Novaseq 6000 sequencing platform to generate 150 bp paired-end reads.