scRNA-seq of mus musculus:PM_204985__
Single-cell suspensions of 1  105 cells/mL in PBS were prepared. Single-cell suspensions were then loaded onto microfluidic devices, and scRNA-seq libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with UMI), reverse transcription of mRNA into cDNA and amplification, cDNA fragmentation, and finally cDNA fragment cloning. Individual libraries were diluted to 4 nM and pooled for sequencing. Pools were sequenced on a NovaSeq 6000 (Illumina, San Diego, USA) with 150 bp paired-end reads.