ONT sequencing of phlebotomus perniciosus
Individual sand flies were homogenised using a pestle and DNA was extracted using Dynabeads SILANE Genomic DNA Kit using the manufacturer's instructions, to produce high genomic weight DNA. The concentration of the DNA was assessed using dsDNA high sensitivity Qubit assay with Fluorometer.  The 4200 TapeStation system, with Genomic DNA ScreenTape assay (Agilent), was used to assess the quality and quantity of DNA, and the sample with the highest molecular weight DNA was selected (80% DNA with peak at 20,000bp).  A DNA library was prepared using the Ligation Sequencing Kit V14 (SQK-LSK114; Oxford Nanopore Technologies (ONT), UK), following the manufacturer's protocol.