GSM9317827: Control_scRNAseq; Mus musculus; RNA-Seq
Tissues were digested with neutral protease, collagenase IV, and DNase I at 37°C for 30 min, filtered through 40 μm mesh, and RBCs were lysed. Subsequently, CD45+ cells were isolated using magnetic bead sorting. Cd45+ cells were loaded onto the 10X Genomics Chromium 3′ v3 platform. Libraries were sequenced on Illumina Novaseq 6000.