GSM8723651: Hep3B-gDNA; Homo sapiens; Bisulfite-Seq
QIAwave DNA Blood & Tissue Kit (qiagen) Genomic DNA (gDNA) was fragmented into 150–200 bp pieces using a Covaris S2 ultrasonicator (Covaris Inc., Woburn, MA, USA). Library preparation utilized the TruSeq® Methyl Capture EPIC Library Prep Kit (Illumina, San Diego, CA, USA) with 500 ng of gDNA. After end-repair, A-tailing, and ligation with methylated adapters, pre-capture libraries were pooled and enriched using Illumina EPIC probe sets targeting over 3.3 million CpG sites through two hybridization and capture rounds. Bisulfite conversion was performed with kit reagents, followed by PCR amplification with Kapa HiFi HotStart Uracil+ polymerase (Kapa Biosystems, London, UK). Library quality and quantity were evaluated using a Qubit DNA Assay Kit and Agilent 2100 Bioanalyzer. Sequencing was conducted on the Illumina NovaSeq 6000 platform.