GSM8971208: 5104; Bos taurus; OTHER
One mL of blood was sent to the Génome Québec sequencing platform for DNA extraction, enzymatic methyl-seq (EM-seq) library preparation and whole-genome sequencing Libraries were pooled at equimolar concentrations and sequenced using Illumina NovaSeq technology, generating approximately 450 million 150-bp paired-end reads per library.During library preparation, there is a DNA conversion step involves in three primary enzymes: TET2 and T4-BGT detect and transform 5mC and 5hmC into a product that cannot be deaminated by the APOBEC3A enzyme. Subsequently, APOBEC3A deaminates unmodified cytosines by converting them to uracils. As a result, unmethylated cytosines are transformed into uracils and then to thymines during PCR amplification, while methylated cytosines remain as cytosines