GSM8945992: stage 1, inactive meristem, pair A; Marchantia polymorpha; RNA-Seq
The RNeasy® Plant Mini Kit was used to extract RNA from homogenised samples. 500 ng of total RNA was used for the construction of sequencing libraries. Poly-A mRNA was isolated with the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490). Enriched poly-A mRNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® following the kit instructions. The fragment size of the libraries was assessed using the NGS HS analysis kit on an Agilent fragment analyzer system. The library concentrations were quantified with a Roche KAPA Kit by qPCR.