GSM9066132: 20842x2 DEN CDHFD 36 week old Female miR146-/- Liver Immune Cells; Mus musculus; RNA-Seq
Mouse liver was processed into a single-cell suspension and then stained with DAPI and FITC-conjugated anti-CD45 antibody. Following staining, cells were washed twice with 1X DPBS with 0.4% BSA (Miltenyi) and FACS sorted on the BD FACSAria Cell Sorter. Sorted cells were washed in 1X DPBS with 0.4% BSA and processed for scRNAseq by the University of Utah High-throughput Genomics Core. 10x Genomics Chromium Next GEM Single Cell  5' v2 Gene Expression Library Preparation