GSM9112823: AngII+HS_28days; Mus musculus; RNA-Seq
Mouse suprarenal abdominal aortas from one control mouse and two AngII plus salt induce mice were cut and digested in a mixed enzyme solution [450 U/mL collagenase type I (Gibco, cat# 17100-017), 125 U/mL collagenase type XI (Millipore Sigma, cat# C7657), 60 U/mL hyaluronidase type I-s (Millipore Sigma, cat# H3506), and 60 U/mL DNase I (Millipore Sigma, cat# DN25)] for 0.5 h at 37℃. The cell suspension was strained through a 40 μm filter and washed twice with PBS, followed by centrifugation at 500g for 5 minutes. The cells were resuspended in DMEM containing 10% FBS, and were subsequently subjected to sc-RNA seq only when the cell viability was greater than 80%. 10× Genomics Cell Ranger software was used to process the raw single-cell RNA-seq data. After finishing the performance of demultiplexing, alignment, filtering, barcode counting, UMI counting, and gene expression information production for each cell, the downstream analysis (mainly including quality control, Uniform Manifold Approximation and Projection (UMAP) dimensional reduction, clustering analysis) were carried out Seurat R package (version 4.0.5).