AB4 (CRX1802933; CRA027028)
CUT&Tag was performed on 300,000 primary Leydig cells with Vazyme CUT&Tag reagents following manufacturer's recommendations (Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro, Vazyme #TD904), with IgG (Abcam, ab172730) and STAT5B (Abcam, ab178941) antibodies.