GSM9031976: Arabidopsis, Columbia H3.1-3×3Flag,Flag, 12DAG, Rep2; Arabidopsis thaliana; ChIP-Seq
1)Crosslink protein-DNA interactions with formaldehyde; 2) Fragment chromatin via sonication or enzymatic digestion; 3) Immunoprecipitate target protein-DNA complexes using specific antibodies; 4) Reverse crosslinking and purify DNA, followed by library construction for sequencing . ChIP-Seq libraries were prepared using Ovation® Ultralow System V2 Library Preparation Kit (PART NO. 0344, 0344NB).