DNBSEQ-G400 sequencing of SAMD01774953
The quality of total RNA was assessed using a Synergy LX (BioTek), QuantiFluor RNA system (Promega), and 5200 Fragment Analyzer (Agilent Technologies). Small RNA libraries were prepared using the MGIEasy Small RNA Library Prep Kit (MGI Tech). Size selection was performed using MGIEasy DNA Clean Beads (MGI Tech). The prepared libraries were quantified with a Qubit 3.0 Fluorometer (Thermo Fisher) and dsDNA HS Assay Kit, and their quality was checked with an Agilent 2100 bioanalyzer and High Sensitivity DNA kit (Agilent). The libraries were then circularized using the MGIEasy Small RNA Library Prep Kit (MGI Tech Co., Ltd), and DNBs were prepared using the DNBSEQ-G400RS Set (MGI Tech).