GSM9001300: 1DPI_rep2_input; Homo sapiens; Human betaherpesvirus 5; OTHER
Total RNA was isolated from latently infected THP-1 cells and treated with rDNase to remove genomic DNA. Poly(A)+ RNA was purified using a Poly(A) Purist Mag Kit (AM1922; Invitrogen). After fragmentation using RNA fragmentation reagents (AM8740; Invitrogen), an anti-m6A antibody (202-003; Synaptic Systems) was used for immunoprecipitation. Both the input and IP samples were subjected to sequencing library preparation using SMARTer Stranded RNA-Seq Kits (634839; Takara)