RNA-seq of Brevundimonas sp. CEF1
Using the Zymo-Seq RiboFree Total RNA Library Kit, rRNA in the total RNA is removed. Then, the RNA is randomly fragmented using divalent cations through an ionic disruption method. Using RNA as the template and random oligonucleotides as primers, cDNA is synthesized. The double-stranded cDNA is purified, followed by double-end repair and introduction of an "A" base at the 3' end and connection of sequencing adapters. AMPure XP beads are used to select cDNA ranging from 400 to 500 bp, and PCR amplification is performed. The PCR products are purified again using AMPure XP beads, and finally, the library is obtained.