GSM9579483: L. monocytogenes NCTC 10887, 22 °C, on corn salad, biol rep 2; Listeria monocytogenes; RNA-Seq
The L. monocytogenes NCTC 10887 cell pellets acquired were resuspended in 1 mL Trizol®. Resuspended cells were transferred into ZR BashingBead Lysis Tubes (0.1 & 0.5 mm beads) (Cat. No. S6012-50, Zymo Research, Freiburg, Germany) and shook over 10 min at 2,700 rpm. Afterwards, RNA extraction was performed as described in Direct-zol RNA Miniprep (w/Trizol) Kit (Cat. No. R2051, Zymo Research, Freiburg, Germany). RNA was eluted with 50 µL ultrapure water and stored at -80 °C. Library preparation, sequencing, and data analysis were performed by Novogene GmbH (Munich, Germany), ribosomal RNA was eliminated from the total RNA, accompanied by ethanol precipitation. For quantification of the library, Qubit and real-time PCR were used, and the Agilent 2100 Bioanalyzer for size distribution detection. The libraries were pooled according to their effective concentration and the required data volume and sequenced on an Illumina NovaSeq 6000 platform.