RNA-Seq Columbia0 replicate 2
Young leaves (~1-1.5 cm) were collected from rosette of 6-weeks-old Columbia WT or homozygous arm-1 mutant (SALK_063839) plants. Total RNA was isolated using TRI reagent and integrity of RNA was checked by agarose gel electrophoresis, concentration was measured using a NanoDrop spectrophotometer. Libraries were prepared by random fragmentation of the cDNA, followed by 5 and 3 adapter ligation (TruSeq Stranded mRNA LT Sample Prep Kit). Adapter-ligated fragments were PCR amplified and gel purified. Fragments were sequenced on the Illumina NovaSeq 6000 Platform by paired-end read sequencing, generating 150 bp read length. Three biological replicates were analyzed