smxl678 mutant Arabidopsis H3 ChIP-seq input
Samples were fixed with 1% formaldehyde (sigma,47608). Chromatin was fragmented to 100-400bp and 5% was taken as input. Immunoprecipitation was performed with antibody-bound beads at 4C for 6h. After washing, IP and input DNA were treated with RNase A (NEB,T3018L) and Proteinase K (NEB,P8107S). DNA was purified using QIAquick PCR Purification Kit (28106). Libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S). Sequencing was performed on Novaseq with PE150.