GSM8635974: OPC, mPFC, repeat2; Mus musculus; RNA-Seq
For acute-isolated OPC, Mice were anesthetized and transcardially perfused with ice-cold HBSS. The brains were isolated with the meninges removed, followed by dissection of the cortex and hippocampus, which were then minced and digested by 1% papain. OPCs were isolated from the resulting cell suspension via immunopanning and directly lysed on the panning plate by Trizol. Poly-A selected method was used to prepare the RNA-seq library. Sequencing was performed on the DNBSEQ platform with SE50 sequencing length