Single-cell seq of melanoma tumors
Single-cell 5 gene expression libraries generated from freshly dissociated metastatic melanoma tumor biopsies using 10x Genomics Chromium NextGEM Single Cell 5 Reagent Kits v2 and V(D)J Reagent Kits v1.1. Libraries include 5 end transcript capture and optional V(D)J enrichment. Sequencing was performed on Illumina NextSeq 500 with 75-cycle kits; Read 1 captured cell barcodes and UMIs, Read 2 captured transcript sequences