ITS2 amplicons from Pocillopora acuta : Symbiodiniaceae pellets IM_11
DNA was extracted from Symbiodiniaceae pellets using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Different members of the Symbiodiniaceae family were characterised based on the Internal Transcribed Spacer 2 (ITS2) rDNA region using the ITSintfor2 (5'-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GAA TTG CAG AAC TCC GTG-3') and ITS2-reverse primers (5'-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGG GAT CCA TAT GCT TAA GTT CAG-3) (Illumina adapters underlined). PCR products were sent off for sequencing by the Australian Genome Research Facility on a Next Generation Sequencing platform (Illumina MiSeq platform).