16S rRNA V4 metagenome from Dendropsophus ebraccatus: Feces
DNA was extracted with DNeasy kits. The 515F and 806R primer pair with barcodes on the forward primer was used to amplify the V4 region of the 16s rRNA gene. The pooled library was cleaned using a Qiaquick PCR purification kit and sent to the Molecular Biology Core Facilities at the Dana Farber Cancer Institute, Harvard University, MA, USA, for sequencing on an Illumina MiSeq using a single-end 250 bp strategy.