16S rRNA seq of feces from male mice
Using the qualified samples as templates, the V3-V4 regions on the 16S rRNA were amplified by paired primers (forward: 5-TACGGRAGGCAGCAG-3; reverse: 5-AGGGTATCTAATCCT-3). With the amplification, indexed adapters were simultaneously inserted into the ends of the 16S rRNA amplicons to generate indexed libraries ready for downstream sequencing on Illumina MiSeq platform.