GSM8578985: Ampulla, post-ovulatory, rep3; Sus scrofa; RNA-Seq
Total RNA was harvested from post ovulatory stage ampulla (POA; n =3) and early luteal stage ampulla (ELA; n =3) tissues using RNeasy mini kit (Qiagen) The total RNA was quantified using NanoDrop ND-100 Spectrophotometer (NanoDrop Technologies, Wilmington, DE) and Qubit 4 Fluorometer (Thermo Fisher Scientific -IN). The quality of RNA was evaluated using the Tapestation 4200TM (Agilent Technologies, Santa Clara, US). The stranded mRNA-Seq libraries were prepared with the KAPA mRNA HyperPrep Kit with QC passed RNA (500 ng) according to the manufacturers protocol. The RNA-Seq Illumina libraries were then sequenced using Illumina NextSeq2000 to generate 40M, 2 x 150 bp reads/sample data.