GSM9321912: Adult mouse cardiomyocytes, MI, AAV9-Rbm22; Mus musculus; ATAC-seq
Adult hearts from the indicated treatments were dissected, cannulated, and dissociated into a single-cell suspension via collagenase digestion (Collagenase type II, Worthington) using a Langendorff apparatus. AMCM yields and the percentage of viable rod-shaped cells were quantified using a hemocytometer. For the ATAC-seq library preparation, 50,000 viable cells were used and collected. The samples were prepared using the Hyperactive ATAC-Seq Library Prep Kit for Illumina (Vazyme) following the manufacturer's protocol. Briefly, the cell pellets were resuspended in the kit-provided cold lysis buffer and incubated on ice for 5 min. Then, the cells were collected and resuspended with 50 μl transposition mixtures according to ATAC Kit instructions. Mixtures were incubated for the PCR reaction. Mixtures were purified with VAHTS DNA Clean Beads (Vazyme) and amplified for 15 cycles using TruePrep Index Kit V2 for Illumina (Vazyme). Amplification products were further purified with VAHTS DNA Clean Beads.