GSM8727688: SEN rep 5; Homo sapiens; RNA-Seq
RNA was isolated using TRIzolTM Reagent from THP1 cells (mono-culture and co-culture) after LPS stimulation according to the manufacturer's instructions. RNA concentration and quality was measured using the Agilent 2100 Bioanalyzer (Total RNA Pico Chip). Preparation of RNA library and mRNA sequencing was completed by Novogene Co. LTD (Sacramento, CA, USA) using the Illumina NovaSeq X Plus Series (PE150) Sequencing System of samples meeting minimum input requirements [amount ≥ 200 ng, RIN>8, purity (A260/280 ≥ 1.8, A260/230 ≥ 1.8)].