GSM9144030: perictyes, 0.1 ng/ml TcdB2 +  0.1 % DMSO 24 h, rep 2; Homo sapiens; RNA-Seq
RNA was isolated in duplicate from cultured pericytes  using Direct-Zol microprep (Zymogen) and suspended in RNase-free water Stranded RNA-seq libraries were constructed with 1 µg of RNA using the NEBNext poly(A) mRNA isolation kit (New England Biolabs) followed directly by the  XGen Broad Range RNA Library Prep kit (IDT).  Each of the libraries was indexed during library construction in order to multiplex for sequencing. Libraries were quantified using a Qubit 4 fluorometer (Invitrogen) and checked for size and quality on an Agilent Bioanalyzer (Agilent Technology).