16S rRNA Archaea gene sequence of kettle hole sediment - KH19
DNA was extracted using a CTAB-phenol-chloroform-isoamyl alcohol protocol (modified after Nercessian et al. (2005)). The V3-V4 region of the 16S rRNA gene was amplified for Archaea using a semi-nested approach with primers 340F-1000R (Gantner et al., 2011) and primers U341-U806R (Sundberg et al., 2013)