DNA-seq of Aspergillus ustus AUSTUSMSU (VKPM F-2030)
Library preparation kit: SMRTbell prep kit 3.0 (PacBio) Sequencing parameters: Library concentration and size were measured with the Qubit HS DNA Kit (Invitrogen) and Fragment Analyzer. Sequencing was conducted on the PacBio Revio system. Demultiplexing and extraction of HiFi reads: Demultiplexing of the sequencing reads was performed with PacBio lima (2.5.1). HiFi reads (CCS reads with a predicted accuracy  Q20) were extracted using SAMtools (1.13) (Danecek et al. 2021) and converted to FASTQ format using PacBio bam2fastq (1.3.1). The quality of FASTQ files was analysed using FastQC software (version 0.11.5-cegat) (Andrews 2010).