GSM9471730: CUT&RUNseq - Mouse Cardiac tissue  12-weeks of age input; Mus musculus; OTHER
RNAseq: Total RNA was extracted from about 10 mg pulverised young and aged mouse cardiac tissue using a RNeasy mini kit. CUT&RUN: CUT&RUN was done using the CUT&RUN assay kit from Cell Signaling Technology (cat. #86652). 5 mg of powdered heart tissue from young and aged mice was used for each antibody reaction. The tissue was fixed in 0.1% formaldehyde methanol-free. RNAseq libraries prepared by Genewiz. CUT&RUN: libraries generated using  the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, cat. #E7645) and NEBNext Multiplex Oligos for Illumina (Index Primers Set 1, cat. #7335).