GSM9543192: Gel_2, Macrophage, H3ac (ChIP); Mus musculus; ChIP-Seq
Cells were subsequently lysed with ice-cold Lysis Buffers iL1 and iL2, and chromatin was sheared in ice-cold Shearing Buffer iS1 supplemented with a protease inhibitor cocktail using a Bioruptor Plus (Diagenode, USA) to yield fragments of 150–350 bp. ChIP-seq libraries were prepared using the NEBNext® UltraTM DNA Library Prep Kit for Illumina following manufacturer's protocols. The chipped DNA was ligated with adaptors. After purification, PCR reaction was done with adaptor-ligated DNA and index primer for multiplexing sequencing. Library was purified by using magnetic beads to remove all reaction components. The size of library was assessed by Agilent 2100 bioanalyzer.