GSM9113211: RF+Aluminum foil_scRNAseq; Homo sapiens; RNA-Seq
For scRNA-seq organoids were dissociated with Papain dissociation system (worthington biochemical). And total 20,000 cells were used for library contruction. For scATAC‑seq, organoids were gently homogenized, and nuclei were isolated using the 10x Genomics Nuclei Isolation Kit (PN‑1000494). Approximately 15,000 nuclei were loaded onto a Chromium Next GEM Chip H, and libraries were constructed. For scRNA-seq, Library prep was performed following 3P v4 (GEM-X)  protocol (10X Genomics). For scATAC-seq, the Chromium Next GEM Single Cell ATAC v2 Library & Gel Bead Kit according to the manufacturer's protocol.