ILB2
The sequencing library was prepared using the Illumina TruSeq Nano DNA LT Library Prep Kit. Amplified DNA fragments were end-repaired using End Repair Mix 2 to remove 5 overhangs, add phosphate groups, and fill in 3 overhangs. An A-base was added to the 3 ends to prevent self-ligation and facilitate adapter ligation, and sequencing adapters with library-specific index sequences were ligated to the 5 ends to enable attachment to the Flow Cell. Adapter-ligated fragments were purified using BECKMAN AMPure XP Beads to remove self-ligated adapters, followed by PCR amplification to enrich the library templates and further purification using BECKMAN AMPure XP Beads. Final fragment selection and purification were performed using 2% agarose gel electrophoresis.