GSM8607156: Homozygous 3; Mus musculus; RNA-Seq
10X Genomics Single cell 3' gene expression V2 and V3 Single cells from TH-MYCN tumor and ex vivo cell culture  samples subjected to RBC ACK lysis were surface stained with anti-TER119-PE (Biolegend) for 30 min at 4°C. Cells were washed once with 2% FBS-PBS (v/v) followed by DAPI staining (1 ug/ml). Flow sorting for live and non-erythroid cells (DAPI-neg/TER119-neg) was performed on a BD FACS Aria III equipped with a 100um nozzle (BD Biosciences, San Jose, CA) instrument. All flow cytometry data were analyzed using FlowJo software (Treestar, San Carlos, CA).