GSM9062065: std, adult, non-activated, replicate 2; Mus musculus; ATAC-seq
all nuclei were extracted by iodixanol gradient and centrifugation at 500g for 5 min at 4C. Nuclei with nuclear Sun1-myc-sfGFP tag were separated from total nuclear population by magnetic immunoprecipitation. Accessible chromatin for all nuclei were excised and adapters were bound by Illumina transposition kit (Illumina). (Tn5 transposition) DNA fragments were recovered with a Qiagen MinElute kit (Qiagen) and barcoded with Illumina UD indexes. DNA fragments were PCR amplified according to Buenrostro et al and Corces et al. Post amplification libraries were cleaned up by AMPure XP beads (ThermoFisher). Libraries were validated by Agilent High Sensitivity DNA kit. ATAC-seq