GSM8658904: UTX_wk8, replicate 3, scRNA-seq; Mus musculus; RNA-Seq
pLNs were hand-picked from NOD mice and cells were labeled by anti-CD45 antibody. DAPI was used to exclude dead cells while sorting. Live CD45+ immune cells were sorted from the pLNs on the Aira sorter and resuspended at 1000 cells/ul in PBS with 2% FBS and 2mM EDTA (FACS buffer) Sequencing library was constructed per manufacturer's protocol (10x Genomics, 3' scRNAseq)