NC1
Cultured NCCs were transfected with the HOXD1 plasmid or control (N = 3) in the complete culture medium. Cells were washed three times with PBS, and total RNA was extracted using TRIZOL (Takara, Japan, cat: 9109). After confirming RNA integrity, library construction and Illumina sequencing were performed on the BGI DNBSEQ high-throughput platform.