GSM9066332: il26KO_GF_biol_rep_3; Danio rerio; RNA-Seq
10-15 guts per replicate were dissected and RNA was extracted using the Single Cell RNA Purification Kit (Norgen, #51800) following the manufacturer's instructions. RNA integrity and concentration were analyzed on the Agilent 4200 Tapestation system using the High Sensitivity RNA ScreenTape Analysis kit (Agilent, #5067-5579). cDNA quality was checked on the Agilent 2100 Bioanalyzer using the Agilent High Sensitivity DNA Kit (Agilent #5067-4626). After quality control, libraries were sequenced with 100-bp paired-end (PE100) reads on the NovaSeq 6000 (Illumina) sequencer. Raw data were checked for quality using FastQC (v0.11.8) and aligned to the reference genome for Danio rerio danRer11 from the Genome Reference Consortium. RNA sequencing libraries were prepared from 500 ng to 1 μg of total RNA using the Illumina TruSeq Stranded mRNA Library Preparation Kit