WGS of Klebsiella pneumoniae: strain 2842, PacBio Sequel II
High-molecular-weight genomic DNA was extracted from Klebsiella pneumoniae strain 2842 using the MagAttract HMW DNA Kit]. DNA integrity was verified by pulsed-field gel electrophoresis (PFGE). A SMRTbell library was prepared from the sheared DNA (target size ~15-20 kb) using the SMRTbell Express Template Prep Kit 2.0. The procedure included DNA damage repair, end-repair, A-tailing, and ligation of blunt adapters. The final library was purified and its size distribution was assessed using the Agilent FemtoPulse system. The library was sequenced on a PacBio Sequel II system using one SMRT Cell 8M with 30-hour movie times to generate HiFi circular consensus sequencing (CCS) reads.