GSM8764228: NC2, Mouse dental papilla cells were transfected with scramble siRNA; Mus musculus; ChIP-Seq
The mDPCs were transfected with Setd2-targeted siRNA or scramble siRNA and cultured for 5 days under odontoblastic induction conditions. CUT&Tag was performed as described previously (Kaya-Okur et al., 2019), with modifications using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme, China, cat. no. TD904). In brief, 1×105 cells were harvested, and after isolating the nuclei, they were incubated with Con A-coated beads at RT for 10 min. After removing the supernatant, the bead-bound cells were resuspended in 50 μL antibody buffer containing 1 μg rabbit anti-H3K36me3 antibody (Abcam) and incubated at 4°C overnight. The beads were then sequentially incubated with anti-rabbit IgG antibody, pA/G-Transposome Mix, and underwent fragmentation. DNA Spike-in was added to the samples. The reaction was terminated, and DNA fragments were extracted using PCI, followed by PCR amplification with indexed P5 and P7 primers. The library products were enriched, quantified, and finally sequenced using the Illumina NovaSeq 6000 platform in PE150 mode.