GSM9399089: KO_3; Mus musculus; RNA-Seq
Whole embryos (E18.5) were frozen in liquid nitrogen and were crushed into powder using a Multi-beads Shocker (Yasui Kikai). Then, total RNA was extracted using ISOGEN II (Nippon Gene). Approximately 500 ng of RNA were ribosomal RNA-depleted using NEBNext rRNA Depletion Kit (New England Biolabs), and converted to Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols.