RNA-seq of Cd5-L-2 (CRX1156799; CRA018277)
The leaves and roots of Cd-treated I. domestica plants were collected and immediately frozen in liquid nitrogen. The total RNA of leaves and roots was extracted using Omega Plant RNA kit (Omega Bio-tek, Guangzhou, China). The quality and concentration of total RNA were detected on a Bioanalyzer 2100 system using an RNA 6000 Nano kit (Agilent, Santa Clara, CA, USA). The NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490) was used to isolate mRNA. The cDNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit (NEB, E7530) according to the manufacturer's instructions. The constructed cDNA libraries of the leaves and roots of I. domestica were sequenced on an Illumina HiSeq 6000 by Gene Denovo Biotechnology Co (Guangzhou, China). After removing adapters, reads containing ploy-N > 10% and low quality reads, the high-quality clean reads were de novo assembled by using Trinity software.