CUT&RUN to assess SWI/SNF occupancy after 8 days of indicuble re-expression of the I315I SMARCB1 silent variant in the G401 cell line.
Chromatin targets were bound by antibodies and cleaved by a pAG-Mnase to enrich for DNA fragments bound by target proteins. Libraries were prepared from eluted DNA using hairpin looped Illumina Nextera adaptors