GSM8657812: Hepatoblastoma sample 37; Homo sapiens; RNA-Seq
Sample preparation for deparafinization and isolation of cells from FFPE tissue sections following 10x Genomics for Fixed RNA seq - protocol CGO000632 Rev B, using gentleMACS Octo dissociator with heaters (Miltenyi, Cat n. 140-004-386.06). Protocol for probe hybridization, GEM generation and barcoding, amplification and library construction as per 10x Genomics Fixed RNA seq protocol CG000527 Rev E, for multiplexed library (16 rxns x 16 BC): 500,000 to 1 Mio nuclei per sample were taken for probe hybridization, followed by sample washing, pooling for pool preparation, and loading of 80,000 nuclei per library.