GSM9690708: Kidney, normal 1, Glomerulus region 2; Homo sapiens; OTHER
5 um sectioned tissue samples from 2 FFPE transplant control pre-implantation biopsies and 3 DKD (class III) biopsies were prepared on slides. Nanostring GeoMx Human Whole Transcriptome Atlas probes were hybridized to the tissue overnight. The fluorescent conjugated morphology markers used were CD10, PanCK, and SYTO 13 nucleic acid stain. Regions of interest were selected on the GeoMx Digital Spatial Profiler. RNA-ID and UMI-containing oligonucleotide tags were UV-cleaved from the WTA panel within the chosen ROIs and collected in a 96-well collection plate. Illumina i5 and i7 dual indexing primers were added to the oligonucleotide tags during PCR to index each ara of illumination uniquely.