GSM9213417: ATAC-Seq J11; Homo sapiens; ATAC-seq
Enteroid monolayers were washed with PBS and treated with Accutase at 25 °C for approximately 20 min to detach cells. The single cell suspension was collected in a low-binding microcentrifuge tube, washed, counted, and ~50,000 cells were resuspended in 100 µL Anti-Freezing Buffer (Advanced DMEM/F12, 10% v/v FBS, 10% DMSO) and stored at -80 °C prior to DNA extraction. After thawing, nuclei were extracted... Cryopreserved cells were thawed, washed with PBS, and prepared following the Omni-ATAC approach (Corces MR et al, Nat Methods 2017, 14:959). Libraries were purified by AMPure XP magnetic beads with double size selection (0.55x and 1.35x), and size distribution determined by High Sensitivity NGS Fragment analysis kit.