GSM8658434: Th1.Red31negative.rep4; Mus musculus; RNA-Seq
Splenic mouse CD4 T cells were cultured in Th2-differentiation media as above, then sorted in Red31- or Red31+ bins, lysed with Trizol LS (Life Technologies) and processed using Direct-zol™ 96-well RNA kit (Zymogen) and processed for bulk RNA-sequencing per standard protocol in the UCSF Genomics Colab, including RNA quantification with an ND-1000 spectrophotometer (NanoDrop) and reverse transcription with SuperScript III First Strand Synthesis Kit (Invitrogen). cDNA from total RNA using the Illumina TruSeq Library Prep Kit.  Single-end 50 base-pair RNA sequencing was performed on the Illumina HiSeq 4000.