RNA-Dt7AL-2 (CRX1177916; CRA018696)
Three days after seed germination, RNA was extracted from coleoptile for strand-specific RNA-seq. Total RNA samples enriched for eukaryotic mRNA by Oligo(dT) magnetic beads and fragmentation buffer for mRNA fragmentation. The library was obtained by reverse transcription, cDNA purification and PCR amplification.