HEK3-ins3-RTT23-PBS7-PE3-1
Genomic DNA samples were amplified with primers specific for Illumina adapters using Phanta Max Super-Fidelity DNA Polymerase (Vazyme). The first round of PCR reactions was carried out with the following parameters: 95 C for 3 min, followed by 23 PCR cycles, and a final 72 C extension for 5 min. In the second round of PCR reactions, 13 cycles were performed. The primers contained Illumina adapters and a 7-bp/8-bp index. The PCR products were purified before sequencing in the Illumina sequencing platform.