GSM9542810: NANOG_Dif_D2_rep3; Homo sapiens; RNA-Seq
Total RNA was extracted following the standard TRIzol protocol (invitrogen). RNA libraries for RNA-seq were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme) following manufacturer's protocols; RNA libraries for amplicon were prepared by specific RT and PCR amplification.