GSM9112986: AO3_S17_L001; Homo sapiens; RNA-Seq
RNA isolation was performed using a NucleoSpin RNA extraction kit (Takara Bio, 740955.25, CA, USA). Three samples of RNA per treatment condition were collected in three independent experiments. RNA integrity was assessed using an Agilent 2100 Bioanalyzer. All samples used for sequencing had an RNA Integrity Number (RIN) of 8.0 or higher, ensuring high-quality RNA suitable for high-resolution transcriptomic analyses (Supplementary Table 5). RNA-seq libraries were sequenced on an Illumina NovaSeq 6000 platform using three lanes of SP flow cells with 51 base pair paired-end (PE) reads. This setup provided high-throughput sequencing with robust data output per sample.