GSM9001444: Fucoidan 6; Salmo salar; RNA-Seq
Total RNA was extracted from samples (6 fish per dietary group) using Qiazol Lysis Reagent (Qiagen) for standard extraction method (based on chloroform and isopropanol extraction). Total RNA was eluted in 30 µL of ultra-pure water and concentration and integrity were measured through Nanodrop (Nanodrop Technologies) and BioAnalyzer (Agilent), respectively. All samples had ≥ 200 ng of mRNA and RIN ≥ 8 Library preparation was performed by NovoGene (Cambridge, UK)