snRNAseq from Mus musculus; hippocampus in home cage, rep 1
freshly dissected hippocampi were dissociated, nuclei were prepared then concentrated in 1X nucleus buffer from 10X Genomics.  1l of nuclei was stained with Trypan Blue (Invitrogen, T10282) and manually counted. In total, 16,00020,000 nuclei were used for the tagmentation reaction and 10x Chromium controller loading. The DNA and RNA libraries were generated according to the manufacturers recommended protocol (https://www.10xgenomics.com/support/single-cell-multiome-atac-plus-gene-expression).