GSM9213575: RNA-seq with RIF1-KO cells (HCT116 LIG1/3-mAID2 RIF1-KO), Rep2; Homo sapiens; RNA-Seq
Total RNA was extracted from cells using illustra RNAspin columns (Cytiva, #25050071). Ribosomal RNA was removed using NEBNext rRNA Depletion Kit v2 (NEB, #E7405L). RNA libraries were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760S), following the manufacturer's instructions. Sequencing was performed on the HiSeq 2500 platform with paired-end 50 bp reads to obtain at least 40 million read pairs per sample.