GSM8945012: RNA-seq of mouse femoral artery (VS2, Rep3); Mus musculus; RNA-Seq
Total RNA was isolated from injured mouse femoral arteries using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. Total RNA was extracted, and mRNA was enriched using oligo(dT) magnetic beads. The mRNA was fragmented and reverse-transcribed to cDNA. After end repair, A-tailing, adaptor ligation, and PCR amplification, libraries were constructed and sequenced on the BGISEQ platform by BGI.