GSM9316818: ATAC-seq, OV, CD70POS, REP 4; Homo sapiens; ATAC-seq
Human kidney tumor from PDX models were digested into single cell suspensions using the Tumor Dissociation Kit, human (Miltenyi Biotec) according to the manufacturer's instructions. Red cell blood lysis was performed using ACK buffer (Lonza). ATAC was performed on cells using the Tagment DNA TDE1 Enzyme (Illumina, #20034198). Sequencing libraries were purified with AMPure XP magnetic beads (Beckman Coulter, A63881), and quantified using a Qubit fluorometer (ThermoFisher Scientific) and a TapeStation (Agilent).