GSM9170966: 26w-WT4; Mus musculus; RNA-Seq
Mice were euthanized at 5, 26, or 52 weeks, and tibialis anterior was dissected and flash-frozen. Total RNA was then extracted using TRIzol, chloroform, and isopropanol. 500 ng RNA per sample was used for library prep and sequencing. RNA libraries for RNA-seq were prepared by the University of Michigan Advanced Genomics Core, using the Illumina platform with manufacturer's protocols for polyA-enrichment.