snRNA-seq_PM_broiler_D2 (CRX1905286; CRA028890)
Single-cell RNA sequencing library construction begins with dissociating tissue into single cells, followed by cell capture using microfluidic systems. Each cell is encapsulated in a droplet with a barcoded bead containing unique molecular identifiers and cell barcodes. Cells are lysed, and mRNAs are reverse-transcribed into cDNA with barcoded primers. The cDNA is then amplified and fragmented, followed by library construction where adapters and sample indices are added. submitted_instrument_model: Illumina NextSeq 2000