GSM8971792: MCF10A_prexasertib_rep1; Homo sapiens; OTHER
Cells were immobilized on concanavalin A-coated beads and permeabilized. GST-RHΔ-MNase to target RNA–DNA hybrids (R-loops) was added. Following enzymatic cleavage at R-loops sites, released genomic DNA was extracted using phenol-chloroform purification and ethanol precipitation. Library was prepared using NEBNext Ultra II DNA Library Prep Kit (NEB) according to the manufacturer's protocol.