GSM8832994: Ovarian Tumour Epithelial cells - 19; Homo sapiens; RNA-Seq
RNA from Brescia Ovarian tumour samples was extracted using Trizol reagent (ThermoFisher Scientific, CA, USA) followed by clean-up with RNeasy MinElute Cleanup Kit (Qiagen, CA, USA) according to manufacturer's instructions. Single cell RNA purification kit (Norgen Biotek Corp., Canada) was used for extracting RNA from ovarian tumour epithelial cells. Libraries from total RNA samples from Brescia samples were prepared using NEB Next® Ultra RNA Library Prep Kit for Illumina (NEB, UK) using manufacturer's recommendations. . SMARTer Stranded total RNA-Seq kit v2 Pico input (Takara Bio USA) was used for ovarian tumour epithelial cells according to manufacturer's protocol.