GSM8579073: MC_H3K27Ac_D2_24h_Buty; Homo sapiens; ChIP-Seq
Total RNA was isolated from butyrate-treated and vehicle-treated PBCMCs using the RNeasy Micro Kit (Qiagen, 74004) according to the manufacturer's protocol. Chromatin was extracted from 100K crosslinked PBCMCs, followed by sonication to obtain fragmented chromatin. Immunoprecipitation was performed using antibodies against H3K27Ac (Abcam, Ab4729) and H3K4Me2 (Abcam, Ab32356) with BSA-blocked Protein A agarose beads (Millipore #16-125). For RNA-Seq, sequencing libraries were prepared from isolated RNA using the TruSeq RNA Library Prep Kit (Illumina). For ChIP-Seq, Illumina sequencing libraries were prepared from immunoprecipitated chromatin using the ThruPLEX DNA-Seq Kit (Rubicon Genomics).