WGS of Klebsiella pneumoniae: strain 2842, Illumina MiSeq
Genomic DNA was extracted from a single colony of Klebsiella pneumoniae strain 2842 using the QIAamp DNA Mini Kit] according to the manufacturer's instructions. The quantity and quality of the DNA were assessed using a Qubit fluorometer and agarose gel electrophoresis. A sequencing library was constructed using the  Illumina Nextera XT DNA Library Preparation Kit] with dual indexing. Briefly, genomic DNA was fragmented, end-repaired, A-tailed, and ligated with Illumina adapters. The library was then purified and amplified with limited-cycle PCR. Final library size distribution and concentration were validated using an Agilent Bioanalyzer 2100 system. The library was sequenced on an Illumina MiSeq platform to generate 2  150 bp paired-end reads.