GSM8727464: PIEZO2FF mouse 9, Cre-, HFD; Mus musculus; RNA-Seq
Total RNA extracted by Trizol and Qiagen RNAeasy kit. 100 ngs total RNA was polyA selected, fragmented and then converted to double stranded cDNA followed by ligation of sequencing adapters. Total RNA samples were prepared into RNAseq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs E7760L) following manufacturer's recommended protocol. Briefly, for each sample 100 ngs total RNA was polyA selected, fragmented and then converted to double stranded cDNA followed by ligation of sequencing adapters.