t6_gla3_mcp200_r2
Samples were pulverized under liquid nitrogen. Total RNA was extracted from three biological replicates using a modified DEPC-CTAB protocol. RNA was quality-checked on an agarose gel and was quantified using a Nanodrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). Following quality validation and quantification using a Qubit Fluorometer (Thermo Fisher Scientific) and Agilent Bioanalyzer (Santa Clara, CA, USA), cDNA libraries were prepared from the RNA and sequenced by the TAMU Genomics & Bioinformatics Service (College Station, TX) on an Illumina NovaSeq platform as 2 x100 bp paired-end reads.