GSM8579764: cKO_0h_4; Mus musculus; RNA-Seq
At 7 days after lentivirus infection, cultured NPCs were treated with actinomycin-D (ActD, 5 μg/ml, Sigma SBR00013) to halt new RNA synthesis. At 0, 3, and 6 hours post-treatment, cells were washed once with PBS and collected with Trizol reagent for total RNA extraction. The concentration and purity of total RNA were assessed through agarose gel electrophoresis and the Agilent 2100 Bioanalyzer system to ensure high-quality RNA. Subsequently, the RNA samples were sent to Beijing Genomics Institute (BGI) for RNA sequencing on the DNBSEQ platform, following their established protocols available on their website (https://www.yuque.com/yangyulan-ayaeq/oupzan/gu8ls7). RNA libraries were prepared  by Beijing Genomics Institute (BGI)  following their established protocols available on their website (https://www.yuque.com/yangyulan-ayaeq/oupzan/gu8ls7).