GSM8514657: MDA-MB-231_Doxorubicin_72H_dup; Homo sapiens; RNA-Seq
For RNA-sequencing, total RNA was extracted from cells using miRNeasy Mini kit (Qiagen; 217004). DNase digestion with the RNase-free DNase set (Qiagen; 79254) was performed to remove DNA. RNA library was prepared based on the protocol of NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs; E7770L). After quality control to confirm the library size using tapestation system (Agilent), the RNA library was sequenced using 150bp paired end read.