Amplicon NGS_HEK293T cells transfected with pDNA expressing Cas9 and a targeting sgRNA against a fluorescent Stoplight reporter sequence
Genomic DNA was extracted from HEK293T cells following either transient transfection or extracellular vesicle (EV) treatment using the Maxwell RSC Cell DNA Purification Kit (Promega, USA). The purified PCR amplicons were submitted to Eurofins Genomics for next-generation sequencing (NGS) using the INVIEW CRISPR Check service. Eurofins carried out adaptor ligation to attach Illumina-compatible sequencing adapters, generating approximately 5 million paired-end reads per sample or sample pool.