RNAseq of Oryza sativa L., black rice variety collected from Udalguri replicate 2
The RNA-Seq paired end sequencing libraries were prepared from the QC passed RNA samplesusing Illumina TruSeq Stranded mRNA sample Prep kit. Briefly, mRNA was enriched from the totalRNA using Poly-T attached magnetic beads, followed by enzymatic fragmentation, 1st strand cDNAconversion using SuperScript II and Act-D mix to facilitate RNA dependent synthesis. The 1ststrand cDNA was then synthesized to second strand using second strand mix. The dscDNA was thenpurified using AMPureXP beads followed by A-tailing, adapter ligation and then enriched bylimited no of PCR cycles.