GSM9064252: Veh_ChIP 2; Homo sapiens; ChIP-Seq
The cells were fixed with 1% formaldehyde for 10 min at room temperature and subsequently quenched with 0.125-M glycine. ChIP was performed with anti-Nrf2 antibody (Cell Signaling Technology; D1Z9C).The antibody incubation were pereformed overnight at 4 °C. The DNA libraries were prepared from 2 ng of ChIP and input samples quantified with a Qubit Fluorometer (Life Technologies), using the NEB kit. Libraries were prepared for sequencing following standard Illumina protocols.