GSM8727711: AdCTL-2; Homo sapiens; RNA-Seq
Total RNA was isolated from HUVECs infected with AdKLF15 or AdCTL using Trizol reagent. According to the instructions of the experiment, the purified Total RNA was separated into mRNA, fragmented, first-strand cDNA synthesis, second-strand cDNA synthesis, end repair, adding A to the 3 'end, ligating connector, enrichment and other steps to complete the construction of sequencing sample library.