GSM9576418: SPG4 cortical organoids; Homo sapiens; RNA-Seq
Organoids were dissociated into single-cell suspensions using Accutase Single-cell suspensions were washed and resuspended in 0.04% BSA in PBS. Cells were counted automatically with Countess III (Thermo Fisher, Waltham, MA) to determine their concentration. Single-cell RNA-sequencing libraries were the prepared using the Chromium GEM-X Single Cell Gene Expression v4 Kit (10X Genomics, Pleasanton, CA) in accordance with the manufacturer's protocol. Briefly, the cells were diluted into the Chromium GEM-X Single Cell 3' Chip as to yield a recovery of ~ 20,000 single-cell. Following the library preparation, the libraries were sequenced in multiplex on the Novaseq X Plus sequencer (Illumina, San Diego, CA) to produce on average a minimum of 50,000 reads per single cell.