GSM8578484: Ethanol 2h replicate 1; Saccharomyces cerevisiae; RNA-Seq
Cells were disrupted using glass beads, and their aqueous content was extracted using phenol/chloroform. Nucleic acids were then precipitated using ethanol and genomic DNA was removed using DNase treatment (Qiagen). RNA was quantified using Qubit (Thermo Scientific) and assessed for quality with a 2100 Bioanalyzer (Agilent Technologies). Poly-A selected transcriptome libraries were generated using the Kapa RNA HyperPrep (Roche).