GSM8945689: sRNA_WT_tas3batch_Rep2; Arabidopsis thaliana; ncRNA-Seq
Total RNA was extracted from 12-day-old Arabidopsis seedlings using the TRIzol method (Takara, 9108). sRNA-seq: Small RNAs (sRNAs) of varying lengths were separated by 15% Urea-PAGE, and the 15-40bp bands were excised post-electrophoresis. The gel slices were soaked overnight at 4℃ in 0.4M NaCl solution, followed by ethanol precipitation.