Metatranscriptome day 12
Total RNA was extracted using the Qiagen RNeasy PowerSoil Total RNA Kit, followed by quantification with a NanoDrop spectrophotometer and integrity assessment via an Agilent Bioanalyzer. Raw sequencing reads were quality-filtered, assembled de novo with Trinity, clustered using CORSET, functionally annotated against multiple databases, and analyzed for differential gene expression to reveal hydrocarbon degradation pathways.