GSM9324143: Non-infected organoid; Homo sapiens; RNA-Seq
hIOs were harvested and dissociated by incubation with TrpLEX (Gibco, ref 12604013) 37 °C for 20min. Single-cell suspensions were loaded onto the 10x Chromium controller according to manufacturer's instructions of the Chromium Next GEM Single Cell 3ʹ Kit v3.1 (10x Genomics, ref 1000269) with a targeted cell recovery of 10,000. Sequencing libraries were prepared according to the manufacturer's instructions. Resulting libraries were pooled and sequenced on an Illumina NextSeq2000.