GSM9112243: 4T1 splenic MDSC, Pooled input; Mus musculus; ChIP-Seq
Cells were fixed for 15min by adding 1/10 volume of 37% formaldehyde (Sigma-Aldrich #F-8775) and quenched with 1/20 volume 2.5M glycine. Cells were washed with 0.5% Igepal/PBS and 1nM PMSF and snap-frozen before delivery to Active Motif. Illumina sequencing libraries were prepared from the ChIP and Input DNAs using the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation using automated system (Apollo 342, Wafergen Biosystems/Takara). After a final PCR amplification, the resulting DNA libraries were quantified and sequenced on Illumina NovaSeq 6000.