RNA-seq of adult whole fly, GR23E10>dec2-WT, rep 2
Drosophila melanogaster were reared on standard cornmeal molasses meduim, under controlled environment with temperature of 25oC and L:D cycle of 12:12. Flies for RNA extraction were collected at ZT3. RNA was extraction from whole animal (~ 70 flies) using standard a TRIzol TM reagent protocol (Thermo Fisher Scientific, cat# 15596018). For illumina sequencing:  After RNA extraction, genomic DNA was removed using a GeneJet RNA-purification kit (Thermo Fisher Scientific, cat# K0702). RNA quality was measured using a Bioanalyzer and around 1.5-2 g of purified RNA with RIN above 6 for each replicates was sent to Novogene (Sacramento, CA) in dry ice for cDNA library prep and sequencing. For each genotype, three independent biological replicates were sequenced.