Hybridization in marsh violets
IGA Technology Services (Udine, Italy) did ddRAD library preparation using a custom protocol, with minor modifications with respect to Petersons ddRAD preparation (Peterson BK, Weber JN, Kay EH et al. Double digest RADseq: An inexpensive method for de novo SNP discovery and genotyping in model and non-model species. PLoS ONE 2012;7:e37135. doi:10.1371/journal.pone.0037135). Enzyme combination (PstI and EcoRI) was selected by analysing in silico the reference genome of Viola odorata L. (sect. Viola; ToLid: ddVioOdor1.1).