Gut metagenome  PKU5_G
Using primers for the V4 region: forward 515F (5 GTGYCAGCMGCCGCGGTAA 3) and reverse 806R (5 GGACTACNVGGGTWTCTAAT 3) and protocols 16S Metagenomic Sequencing Library Preparation Protocol (Illumina). The library was double-indexed using the Nextera XT kit.