GSM8516620: H1299, 2, HOXD1, TBS; Homo sapiens; Bisulfite-Seq
1 μg of genomic DNA was converted using the ZYMO EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA) and one twentieth of the elution products were used as templates for PCR amplification with 35 cycles using KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems, Wilmington, MA, USA) For each sample, BSP products of multiple genes were pooled equally, 5'-phosphorylated, 3'-dA-tailed and ligated to barcoded adapter using T4 DNA ligase (NEB). Barcoded libraries from all samples were sequenced on Illumina platform.