GSM8516349: DFCI SINET8 H3K27me3; Homo sapiens; ChIP-Seq
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). ChIP-seq libraries were prepared using the ThruPLEX DNA-seq Kit (Rubicon Genomics, R400427) as recommended. Libraries were validated using the Agilent High Sensitivity DNA Kit.