Meiotic double strand DNA breaks and spontaneous mutation in Drosophila melanogaster: Line 1
Upon completion of the 30th generation of MA, we collected all female offspring (ranging from 6-24 individuals per line) and froze them at 80 C. We extracted genomic DNA from stored flies using the Qiagen DNeasy Blood & Tissue Kit with the insect protocol (catalog #69504), purified using the Qiagen DNeasy PowerClean Kit (catalog #12877-50 ), and quantified using a Qubit Fluorometer (ThermoFisher Scientific). We submitted DNA to the University of Wisconsin Biotechnology Center DNA Sequencing Core Facility (RRID:SCR_017759) for paired-end whole genome sequencing using an Illumina NovaSeq X Plus.