Oxford Nanopore Technology sequencing of 30 laboratory females
Genomic DNA was extracted using the Nucleobond AXG20 kit and buffer set IV from Macherey-Nagel (ref. 740544 and 740604, https://www.mn-net.com, Dren, Germany). DNA fragments less than 10-kb were eliminated using the SRE XS kit from Circulomics (https://www.circulomics.com/, Baltimore, Maryland, USA). The ligation sequencing kit SQK-LSK109 (ONT) was then used to prepare the sample, which was purified and repaired with magnetic beads, and adapters (barcoding kits NBD104 and EXP-NBD114) were ligated on DNA fragments.