GSM9066127: 18m-3; Bos grunniens; RNA-Seq
Grind the frozen tissue into powder using a mortar and pestle in liquid nitrogen, and then dissolve it in 400 μ L lysis buffer. After RNA extraction from tissue samples, Nanodrop was used to detect RNA purity, and Qubit was used to accurately quantify RNA. After passing the RNA sample detection, the Covaris ultrasonic disruptor is used to randomly interrupt the entire library preparation process, followed by end repair, addition of A tail, addition of sequencing adapter, purification, PCR amplification, and other steps. After passing the library detection, different libraries are pooled into Flow cells and cBOT clusters according to the requirements of effective concentration and target data volume. Then, Illumina NovaSeq high-throughput sequencing platform is used for sequencing.