16S rRNA gene sequencing of adult gut microbiota
Fecal material was collected on toilet paper from adult mood/anxiety patients at baseline and following 3-months of supplementation of a delayed-release apple cider vinegar product. Soiled toilet paper sections were dissected, DNA was extracted using the QIAGEN power soil HTP kit, 16S hyper variable region V3-V4 (341-805 bp) was PCR amplified, sequenced on Illumina Miseq.