GSM8578404: TED4; Homo sapiens; miRNA-Seq
Total RNA was extracted from orbital connective tissues by using TRIzol. Then RNA was quantified with a NanoDrop 2000. RNA sequencing libraries were constructed with 1μg high-quality RNA of each sample according to the instructions of TruSeq Small RNA Library Preparation Kit (Illumina) for sequencing. The libraries were sequenced on an Illumina NextSeq 550 sequencer.