GSM8832020: Col-0 SUC2:.CO:CIT aba1-6 ChIP biol rep.2; Arabidopsis thaliana; ChIP-Seq
5 grams (fresh weight) of whole seedlings were used for each genotype/replicate. A bioruptor sonicator (Diagenode, B01020001) was used to shear the chromatin using the following settings: 40 Cycles, 30 sec ON, 30 sec OFF at high power. With these conditions the size of sheared DNA is below 100 bp. GFP-Trap magnetic nanobodies (Chromotek) were used. Inputs and ChIP samples (two biological replicates) were quantified with Qubit dsDNA Quantification High sensitivity Assay Kit (Invitrogen). For library preparation, the VAHTS Universal Plus DNA Library Prep Kit for Illumina (ND617-02, Vazyme, Nanjing, China) was used, following the manufacturer's recommendations without fragmentation. Library quality control was performed using the QSep-400 (BiOptic, Taipei, China) and Qubit 3.0 (Thermo Fisher Scientific, Wilmington, USA). The qualified library was sequenced on the Illumina Novaseq X platform (Illumina, San Diego, USA) with paired-end 150 bp (PE150) mode. Library construction and sequencing were performed at Biomarker Technologies (BMKGENE) GmbH.