RNA-Seq of Lycium ruthenicum:fruit
Total RNA was isolated, and mRNA was enriched using oligo(dT) magnetic beads. The enriched mRNA was fragmented, and double-stranded cDNA was synthesized using random hexamers. After end repair, 6' end dA-tailing, and adaptor ligation, the ligation products were amplified by PCR to construct the final sequencing library.