GSM9033031: MV411-shUBR5_F6_RNA_KO539_25nM_3d_REP1_240626_NANOVO_S37; Homo sapiens; RNA-Seq
For cell line experiments, RNA was extracted using the RNeasy Mini kit (Qiagen). For RT–qPCR, cDNA was synthesized using the Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit. RNA integrity was evaluated using the Agilent TapeStation 4200 (Agilent Technologies), and libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (New England Biolabs). Sequencing was performed on an Illumina NextSeq™ 550 system (Illumina), producing 37-bp, paired-end reads.