GSM8833058: MSI2-KO, replicate 2; Homo sapiens; RNA-Seq
72 hours after editing, viable cells (negative for DAPI) were sorted with a 100 um nozzle - on a FACSymphony S6 Cell Sorter (BD Biosciences) - according to the surface expression of CD34+CD45RA-CD90+ markers. The sorted cells were counted and roughly 16,000 cells from each sample were utilized for the subsequent procedure (estimated recovery: 10,000 cells/sample). Libraries were prepared by Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (10X Genomics) according to manufacturer's instructions.