GSM8658974: Fibroblasts, UUO, rep3; Mus musculus; Bisulfite-Seq
Genomic DNA was extracted from FACS-sorted fibroblasts using TIANamp Micro DNA Kit (TIANGEN) . Genomic DNA was subjected to RRBS library preparation using the Acegen Rapid Library Prep Kit(Acegen) and then bisulfite-treated using EZ DNA Methylation Gold Kit (Zymo Research). After bisulfite treatment, DNA  was amplified with 12 cycles of PCR. Post amplification libraries were size selected at 100-350bp in length using a dual-SPRI® protocol.