16S rRNA gene sequencing from faecal content of Sus scrofa domesticus: three distinct times post-weaning
Microbial genomic DNA was extracted from 250 mg of pig fecal samples using the QIAamp PowerFecal DNA kit (Qiagen, Venlo, Netherlands). The hypervariable regions V3-V4 of the 16S rRNA gene were amplified using the 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3) primers following a two-step PCR procedure