GSM8901211: Lung Tumor TAT-CRE; Mus musculus; RNA-Seq
A piece of the tumor tissue with a diameter of 3-5 mm was embedded in Sample Preparation Buffer (Singleron Biotechnologies) stable for up to 72 hours prior processing. Single cell suspension from tumor tissue were processed in sCelLiVETM Tissue Dissociation Buffer. Single cells were loaded into the microfluidic SCOPE-chipTM and cell identifying tags were flowed in and allowed to settle in the wells on top. mRNA from each lysed cell was hybridized to the barcode sequences on the bead in the same well. Then, reverse transcription of primed RNA and cDNA amplification take place. The amplified cDNA was fragmented, adapter ligated and amplified to construct a sequencing library suitable for the Illumina sequencing platform.