ONT WGS of Bordetella bronchialis isolated from CF patient sputum
Genomic DNA was extracted from B. bronchialis cultures using the GenFind V3 Reagent Kit (Beckman Coulter) as per manufacturers instructions. Long-read Nanopore sequencing was performed with a GridION device on FLO-MIN114 R10.4.1 flow cells (Oxford Nanopore Technologies). Sequencing libraries were barcoded with the native barcoding kit SQK-RBK114.96 in accordance with the protocol RBK_9176_v114_revM_27Nov2022.