GSM9399098: iPSC-derived neurons, disomic, Cell line C9, Library C9N061224-4W-V-1; Homo sapiens; RNA-Seq
Total RNA was extracted from ~2-10 mg of fetal human brain tissue using the RNeasy Plus Micro kit (Qiagen) according to manufacturer's instructions. In vitro differentiated NPC or neuron cultures (neurons matured to day 30) were dissociated with Accutase, pelleted, and flash frozen. Samples were stored in -80C until further processing. Library preparation and bulk-RNA-seq were performed using AccuraCode® RNAseq Kit (Singleron Biotechnologies).