16S rRNA amplicon from Emiliania huxleyi obtained from the Bigelow Laboratory for Ocean Sciences (National Center for Marine Algae and Microbiota, https://ncma.bigelow.org/)
total DNA was extracted from 50mL Emiliania huxleyi samples using DNeasy PowerSoil Isolation Kit (QIAGEN Cat. No: 47014). The quality and quantity of DNA were checked on agarose gel (8% w/v) and through NanoDrop ND-1000 spectrophotometer. The 16S rRNA gene were paired-end sequenced through Illumina HiSeq 2500 platform to investigate the composition of the microbial community by BGI (Beijing Genomics Institute, Shenzhen, China).