Genome of Pseudomonas sp. Hg5Tf, PacBio Sequel IIe reads, .fastq file
DNA was isolated from 24 h cultures of Hg5Tf grown in lysogenic broth with 0.01% Hg added as HgCl2, using a column-based commercial kit (A&A Biotechnology, cat. #: 060-60) avoiding centrifugations except the elution step, which was done in 50 microliters of DEPC-treated nuclease free water (A&A Biotechnology, cat. #: 003-075). Isolated DNA was purified by the salting-out method by adding 5 microliters of 3M CH3COONa and 110 microliters of 96% ethanol for 50 microliters of sample, then freezing in -80 degrees Celsius for 12 days. After that the samples were centrifuged (60 min, 15 000 rcf, at 4 degrees Celsius), washed twice with 125 microliters of 96% ethanol and resuspended in 40 microliters DEPC-treated nuclease free water (A&A Biotechnology, cat. #: 003-075). The PacBio Microbial Library was prepared from the DNA sample and sequenced using PacBio Sequel IIe with 200 Mb (CCS/HiFi) reads per sample at Macrogen.