May normoxia replicate 4
After acclimation to 8C in an incubator, the single copepod was sealed in a 500-ul well of a microrespirometer plate, containing 400 ul of filtered seawater to allow for an large air bubble. Dissolved oxygen was monitored in an incubator at 8C, and the individual was transferred to Trizol after 2 hours (while oxygen was still > 5 mg O2 l-1). Total RNA was isolated with Zymo Directzol, and library prepared with Lexogen 3 Quantseq FWD kit.