GSM8516299: Hepatocytes, G-MDSC depletion, POD2, rep3; Mus musculus; RNA-Seq
Hepatocytes from BALB/C mice livers were extracted one or two days after hepatectomy using the two step perfusion method: mice were anaesthetized and a cannula was inserted into the inferior vena cava. The portal vein was cut, and the mouse liver was perfused with perfusion medium (Ca2+/Mg2+ ion-free HBSS buffer containing 5 mM EGTA, 25 mM HEPES) for 5 minutes and then with the addition of 0.8 mg/mL type-I collagenase, at 37 ºC for another 8 minutes. Following adequate digestion, the liver was resected and passed through a 70 μm filter, followed by centrifugation of the cells for 2 min at 50 G. RNA from the hepatocyte pellet was extracted using the QIAGEN RNAeasy mini-kit. Inhouse poly A based RNA seq