GSM9170982: snRNA-AR113Q2; Mus musculus; RNA-Seq
Mice were euthanized at 52 weeks and tibialis anterior was dissected and flash-frozen for storage. Nuclei were then isolated with the 10X Chromium Nuclei Isolation kit and suspended in individual droplets. Approximately 10,000 nuclei per sample were sent through the 10X platform for sequencing. RNA libraries were prepared by the University of Michigan Advanced Genomics Core, with the pool subjected to 2 x 150bp paired-end sequencing per manufacturer's instructions (Illumina NovaSeq).