bacteria
The DNA library was constructed through random fragmentation and adapter ligation. Sequence quality was assessed using FastQC v0.11.5, and adapter trimming was performed with Trimmomatic v0.36. Cleaned reads were evaluated for base quality metrics, GC content, and read count. Assembly was performed using SPAdes v3.15.5 with multiple k-mer sizes, supported by k-mer profiling via Jellyfish v2.2.10. The De Bruijn graph-based assembly generated 14,106,374 reads with a GC content of 44.54% and a Q30 score of 92.18%. Genome validation using mapping and BUSCO analysis produced 12 contigs of 3,231,781 bp with an N50 of 662,366.