GSM8971908: S2; Mus musculus; RNA-Seq
RNA was isolated from CD11b-positive microglial cells by using Direct-zol RNA Miniprep Plus (Product #: R2072; Zymo Research, USA) according to manufacturer's instruction. The isolated RNA was immediately frozen at -80°C and sent to sequence RNA-seq library preparation was performed using Poly(A) selection, where polyadenylated mRNA transcripts were selectively enriched and the sequencing was performed in the Illumina's NovaSeq platform with 30 million read pairs per sample.