GSM8898492: SW480 NDF-KO, WT_Rescue_Rep1; Homo sapiens; OTHER
After labeling, cells were quickly washed with 1× cold PBS, residual liquid was quickly removed from the culture plate, and 3 ml Trizol LS (ThermoFisher) was added directly to the cells. RNA was purified twice using a standard Trizol RNA extraction protocol. RNA libraries were prepared with the NEBNext Small RNA Library Prep Set (NEB) according to kit instructions.