Oestrogen influences class switched B cell frequency exclusively in individuals with an XX sex chromosome complement
Oestrogen influences class switched B cell frequency exclusively in individuals with an XX sex chromosome complement
Peripheral blood was obtained by venipuncture, followed by PBMC isolation via Ficoll gradient. Cells were frozen in liquid nitrogen until use. Thawed PBMCs were sorted using FACS to select total CD19+ B cells PicoPure RNA extraction kit as per manufacturer's protocol Libraries were prepped with either the KAPA mRNA HyperPrep Kit or the TruSeq Stranded mRNA Library prep.