GSM9112672: WT_Ex_3; Mus musculus; RNA-Seq
Total RNA was extracted from approximately 15-20 mg of heart tissue  using TRIzol (Thermo Fisher Scientific), purified with RNeasy Mini spin columns (Qiagen). RNA was eluted using Rnase-free water. Libraries were constructed from polyA-selected RNA using a NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) using manufacturer protocols and sequenced on Illumina HiSeq2500 instrument.