GSM8728042: RT4 Rep2; Homo sapiens; ATAC-seq
Transposed DNA was purified using the MiniElute Reaction Cleanup kit (Qiagen) and eluted in 21 μL elution buffer. Partially amplified libraries were generated by amplifying the purified transposed DNA for 5 cycles using NEBNext High-Fidelity 2X polymerase chain reaction (PCR) Master Mix (New England Biolabs). Partially amplified libraries were quantified by quantitative PCR  to determine the cycle number at which each sample produced 25% of maximum fluorescent intensity. The fluorescent intensity was used to estimate the number of additional PCR cycles required for each sample. Fully amplified libraries were purified using the SPRIselect beads (Beckman Coulter).