GSM8465496: mouse 6, ALY; Mus musculus; RNA-Seq
RNA was extracted using PureLink™ RNA Mini Kit (Thermo 12183018). 1 ug of RNA was used for the construction of sequencing libraries. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. For the directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.