Fleas from Household 31, Dog A, Pool 4
A Pacific Biosciences HiFi library was prepared according to PN                 102-359-000 Version 03 (Pacific Biosciences). Modifications include performing                 upfront PCR with PacBio 16s primers: 27F (Forward Primer):                 5'-GCATCTAGRGTTYGATYMTGGCTCAG-3', 1492R (Reverse Primer):                 5'-GCATCRGYTACCTTGTTACGACTT-3' and KAPA HiFi HotStart ReadyMix (Roche) and a                 purification using AxyPrep Mag PCR beads prior to pooling. Library quality was                 assessed using the Agilent TapeStation System. Library was quantified using the                 Qubit dsDNA High Sensitivity kit. The library was sequenced on a Sequel II using                 Sequel Polymerase Binding Kit 2.88