Metagenomics:human gut
Metagenomic sequencing was performed using the 2b-RAD-M technique, which employs type IIB restriction endonucleases to generate isoschizomers for microbial community analysis. Library preparation and sequencing were conducted on an Illumina platform with paired-end reads of 178 bp. Sequencing depth and coverage were assessed to ensure adequate representation of microbial diversity.