Isolation and Characterization of Broad-Host-Range S. aureus Bacteriophages with Antibiofilm and Cross-Species Activity
Long-read sequencing was conducted in house using the Oxford Nanopore Technologies (ONT) platform on a MinION Mk1B (Oxford Nanopore Technologies, Oxford, UK) device. Specifically, the Rapid Barcoding Kit (Oxford Nanopore Technology, SQK-RBK 114.96) was used to sequence E. faecalis whole genomes on R10.4.1 MinION flow cells (Oxford Nanopore Technology), using 50 ng of the isolated DNA. Base-calling was conducted with Dorado v0.5.0, using the r10.4.1_e8.2_400bps_sup@v4.2.0 basecaller model (Oxford Nanopore Technology).