GSM8865300: Hypo1; Mus musculus; RNA-Seq
Isolated tissues were transferred to 100 mL of lysis buffer (RNAqueous™-Micro Total RNA Isolation Kit, Thermo Fisher, AM1931) and stored at -80°C before processing for mRNA preparation. Six retinal mouse samples (isolated RNA)—3 Hypo and 3 Eugly—were evaluated for RNA quality and subjected to RNA-Seq analysis. RNA quality was assessed on a Fragment Analyzer (Agilent Technologies) and all RNAs had a RQN between 3.8 and 5.5 and . RNA-seq libraries were prepared using 500 ng of total RNA with the Illumina TruSeq Stranded mRNA reagents (Illumina; San Diego, California, USA) following manufacturer's recommendations. Libraries were quantified by a fluorimetric method and their quality assessed on a Fragment Analyzer (Agilent Technologies). Cluster generation was performed with 2 nM of an equimolar pool from the resulting libraries using the Illumina HiSeq 3000/4000 SR Cluster Kit reagents and sequenced on the Illumina HiSeq 4000 using HiSeq 3000/4000 SBS Kit reagents for 150 cycles.