RNA-Seq of Influenza A virus infected THP-1 cells at different timepoint
RNA-Seq of Influenza A virus infected THP-1 cells at different timepoint
THP-1 cells are collected from T75 flasks after IAV infection by centrifuge total RNA was extracted by using Norgen single cell RNA purification kit 125ng of total RNA was processed using the Illumina TruSeq Stranded mRNA kit (cat. 20020595), according to the manufacturer protocol, with 14 cycles of final PCR amplification.