RNA-seq of homo sapiens
RNA sequencing was performed to quantify transcriptomic profiles and provide datasets for further differential analysis under experimental conditions.  Isolation of total cellular RNA was performed utilizing the TRIzol reagent, strictly adhering to the protocol provided by the manufacturer, and RNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, California, USA) to determine the RNA integrity number (RIN; RIN > 8.0) via the Agilent 2100 Expert software (vB.02.10).  Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit and sequenced on an Illumina NovaSeq 6000 platform (Illumina, California, USA) (150 bp paired-end reads).