RNA-seq of MCF-7 co-cultured with ASDSs: co-culture sample 2
RNA extraction was performed using TRIzol reagent kit (Thermo Fisher Scientific, USA). RNA concentration was measured using a Biospec-nano Spectrophotometer (Shimadzu, Japan). Library preparation and paired-end sequencing were performed by Novogene Co., LTD (Beijing, China). Briefly, messenger RNA was isolated using poly-T oligo-attached magnetic beads, followed by cDNA library synthesis, PCR amplification, and purifica-tion.