Small RNA-seq of Culex mosquitoes from Antioquia, Colombia
Mosquitoes were field sampled and taxonomically identified at the University of Antioquia. The specimens were then transported to the Arbovirology Laboratory in the Department of Tropical Medicine at Tulane University for further processing. Total RNA was extracted using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research). Small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) following the manufacturers protocol. Sequencing was performed on the Nextseq 2000 Illumina platform as single reads.