GSM8865008: GM47 D100 sAD E3_TMP_2_biolRepl_1; Homo sapiens; RNA-Seq
RNA was isolated with RNABlue (TopBio, R013)  combined with column extraction with Dnase I pretreatment (Direct-zol RNA Microprep kit (ZymoResearch)). 300 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared with QuantSeq FWD 3'mRNA Library Prep Kit (Lexogen) in combination with UMI Second Strand Synthesis Module for QuantSeq FWD and Lexogen i5 6 nt Unique Dual Indexing Add-on Kit (Lexogen) with 14 - 18x PCR cycles according to the manufacturer's instructions.