GSM8636050: MV4-11 VEN+CSA 24H-1; Homo sapiens; RNA-Seq
Total RNA was extracted from cells or tissues using the Total RNA Extractor (Trizol) reagent according to the manufacturer's instructions. The samples were lysed, and RNA was separated by adding chloroform, followed by centrifugation. For RNA sequencing library preparation, 1 µg of RNA was used and processed using the KAPA RNA HyperPrep Kit (Kapa Biosystems, Wilmington, USA). The libraries were sequenced using an Illumina platform with a 150-bp paired-end configuration.