Single cell level genomic on-target editing efficiency assessment of human (CAR) T cells that have been adenine base edited to a achieve a (functional) knockout of 20 distinct genes simultaneously
A custom Tapestri amplicon panel was designed by Mission Bio to capture 56 potential ABE target regions across the human genome including the 20 distinct target genes that have been edited in these samples (CD3E, B2M, CIITA, PVR, CD52, ICAM1, CD58, PDCD1, CTLA4, CD5, RASA2, MED12, ZC3H12A, RC3H1, TET2, ARID1A, SMARCC1, SMARCA4, SMYD3, ADORA2A). The sample represents UTD T cells of one donor that have been 20-plex edited with a medium dose of gRNAs (0.38g per gRNA)