GSM9112321: C202.2: CD8b+/Thy1.1+ lymph node T cells bearing the NP202 TCR; Mus musculus; RNA-Seq
Total RNA from the samples was isolated using the BD Arcturus PicoPure kit with on-column DNase digestion. Libraries were prepared using the SMART-Seq v4 Ultra Low Input kit with Tagmentation (Takara Bio USA, Inc.), Illumina adapters were added, and each sample library sequenced on the NovaSeq X Plus platform (2 x 250 bp paired end sequencing, 40 x 106 reads/sample).