GSM9317701: Streptomyces coelicolor A3(2) wild type replicate 2; Streptomyces coelicolor; OTHER
Spores were collected in 3 mL of dH2O. Spores were spun down at 13,000 rpm for 1 minute, supernatant was discarded and the spores were resuspended in 978 uL of Sodium Phosphate Buffer from the FastDNA Spin Kit for Soil (MP Biomedicals Germany GmbH). DNA was extracted as per the manufacturers' protocols for the kit except homogenization in the FastPrep Instrument was performed for 40 seconds at speed setting 6, twice. Illumina sequencing libraries were prepared using the tagmentation-based and PCR-based Illumina DNA Prep kit and custom IDT 10 bp unique dual indices (UDI) with a target insert size of 280 bp. No additional DNA fragmentation or size selection steps were performed.