GSM8700542: ATACseq_167dpi_Cl13_Tem2_Rep3; Mus musculus; ATAC-seq
CD8s were stained with viability dye, GP33 tetramer, and surface antibodies. Live CD8+ Tetramer+ T cell subsets were sorted on a combination of surface antigens with a BD FACSAria RPMI + 10-50% RPMI. Nuclei were isolated by spinning at 4ºC 600rcf for 10 minutes in Lysis Buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCL, 3 mM MgCl2, 0.1% Tween-20, 0.1% IGEPAL, 0.01% Digitonin, 1% BSA). DNA was tagmented in transposition mix (Illumina 20034198) at 37ºC for 30 minutes with agitation at 400rpm. DNA was isolated (Qiagen 28004) amplified with Illumina adapters.