RNA-Seq of Arabidopsis: 35S translatome CK1
The RNA was extracted from immunoprecipitation of ribosome complexes from p35S:HF-RPL18 line. The quality and integrity translatome were determined by Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit chip (Agilent Genomics), only translatome with RNA integrity number greater than 8 were used for RNA library construction. Strand-specific RNA sequencing libraries were constructed using Illumina TruSeq Stranded mRNA Library Prep Kit following the protocol from the manufacture with several modifications to adapt to the low RNA input. The RNAs input were 500 ng for p35S:HF-RPL18 line.