XY52Y-1
The genomic DNA of strains was extracted using an improved phenol-chloroform method. DNA concentration was measured using a Qubit 3.0 Flurometer and DNA integrity was checked via agarose gel electrophoresis. Using the Watchmaker Kit, 200 ng of each DNA sample was prepared into a library with fragments of ~350 bp in size. Preliminary quantification was done with Qubit 3.0, followed by fragment size analysis. After confirming quality, qPCR ensured library standards. Sequencing was then performed on the DNBSEQ-T7 platform using the double-end sequencing program to obtain 152bp reads.