GSM9212128: LS174T-R_H3K4me3,LS174T resistant, H3K4me3 ChIP-seq; Homo sapiens; ChIP-Seq
Total RNA was extracted using RNeasy Mini Kit (Qiagen). For ChIP-seq, chromatin was cross-linked with 1% formaldehyde, sonicated, and immunoprecipitated with H3K27ac (Abcam ab4729) or H3K4me3 (Millipore 07-473) antibodies. RNA-seq libraries were prepared with TruSeq Stranded RNA HT kit with Ribo-Zero Gold (Illumina). ChIP-seq libraries were prepared using NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina.