GSM9398946: IVR10-2014 - Rep2; Caenorhabditis elegans; RNA-Seq
L4 stage worms were homogenized using a FastPrep-24 (MP Biomedicals, Irvine, USA) with lysis matrix D; three runs of 10 seconds at 6 m/s were performed, with 2 minutes of cooling on ice between runs. Subsequently, RNA was extracted using RNeasy Mini Kit (Qiagen, Hilden, Germany). RNAseq libraries were constructed from 1000 ng of total RNA at the GeT‐TRiX facility (GénoToul, Génopole Toulouse Midi-Pyrénées) using Illumina® Stranded mRNA Prep kit (Illumina, San Diego, CA, USA) following the manufacturer's instructions. Briefly, mRNAs were captures, then fragmented, converted to double stranded cDNAs and sequencing adaptors were ligated. Libraries were amplified with eleven cycles of PCR.