GSM8549095: RRBS_NBH_C_1; Fundulus heteroclitus; Bisulfite-Seq
Total RNA and DNA were isolated using ZR-Duet DNA/RNA Mini Prep kit (Zymo Research, California). RNA and DNA were checked for quality using Bioanalyzer and Tape station respectively. Gene expression libraries were prepared usiig Illumina External HiSeq2500 High Output SE 50 were sequenced; DNA methylation was conducted using Reduced Representation Bisulfite Sequencing. Library preparations were done using Premium RRBS kit (Diagenode) and sequenced using Illumina Hi-Seq 4000 PE 50bp reads