GSM9033056: IgG CUT&RUN, Dox, rep1; Mus musculus; OTHER
Cells were bound on ConA beads and incubate with the primary antibody or proteins overnight in antibody buffer. For CUT&RUN, cells were incubated with pA-MNase and digested on ice-water in buffer containing CaCl2. Digestion was stopped by 2X STOP buffer and DNA were recovered by high speed spin, Proteinase K/SDS digestion and PCI extraction. For MapR, cell were directly applied for digestion and the rest step as described in CUT&RUN. For CUT&RUN and MapR, NEBNext Ultra II DNA library prep was used for library construction with TruSeq adapter and 14 cycle for library amplification