GSM8606391: Ms#3650_R265-71del+/+; Trp53f/f PbCre+_AD_Case_Lib2; Mus musculus; RNA-Seq
Briefly, single-cell suspensions were loaded onto a Chromium Next GEM Chip along with reverse transcription reagents and Gel Beads containing barcoded oligonucleotides. The chip was then placed in a Chromium Controller to generate Gel Bead-in-Emulsions (GEMs), encapsulating individual cells with unique barcoded Gel Beads. Within each GEM, cellular mRNAs were reverse transcribed to generate barcoded full-length cDNA. Following reverse transcription, the GEMs were broken, and the barcoded cDNA was amplified by PCR. The amplified cDNA underwent fragmentation, end repair, and A-tailing, followed by adapter ligation and PCR amplification to generate final sequencing libraries. Library quality was assessed using an Agilent Bioanalyzer.