GSM9065019: VP161 blood 4; Pasteurella multocida; Tn-Seq
Genomic DNA was extracted using the HiYield Genomic DNA Mini Kit (Real Biotech Corporation) as per the manufacturer's instructions for extraction from Gram-negative bacteria Genomic DNA was fragmented to ~300 bp using Covaris sonication. The fragment DNA was end-repaired and A-tailed before adapters were ligated onto all fragments. Amplification of transposon-chromosome junctions was performed using Kapa HiFi HotStart ReadyMix, a transposon-specific primer and an adapter-specific primer.