GSM9321008: anti-HER2/neu and non-transduced T-cells (n=4 pool each); Homo sapiens; RNA-Seq
Cells were harvested following tumor co-culture, washed in PBS, stained with BD SampleTag antibodies, and counted. Single-cell suspensions were loaded onto the BD Rhapsody platform according to the manufacturer's instructions. Library was performed according to the manufacter's instructions (BD Rhapsody™ System Targeted mRNA and Sample Tag Library Preparation Protocol).