GSM9065059: -P-HT root - 2; Lotus japonicus; RNA-Seq
RNA extraction of shoot and root samples were performed using Favorgen Plant Total RNA Mini Kit (Favorgen Biotech, Ping-Tung, Taiwan), following the manufacturer's protocol. Library preparation was performed by Rhelixa (Tokyo, Japan) using NEBNext® Poly(A) mRNA Magnetic Isolation Module (for PolyA selection; New England Biolabs, Ipswich, MA, USA) and NEBNext® Ultra™ ll Directional RNA Library Prep Kit (New England Biolabs). Sequencing was performed by Rhelixa using the NovaSeq 6000 (Illumina; San Diego, CA, USA) with 150 bp paired end.