RNA-seq of RD cells: XBP1 shRNA 3
Total RNA from control, IRE1a, or XBP1 knockdown RD cells was extracted using TRIzol reagent (Thermo Fisher Scientific) using RNeasy Mini Kit (QIAGEN) according to the manufacturers protocols. The mRNA-seq library was prepared using poly (A)-tailed enriched mRNA at the UT Cancer Genomics Center using the KAPA mRNA HyperPrep Kit protocol (KK8581, Roche, Holding AG, Switzerland) and KAPA Unique Dual-indexed Adapter kit (KK8727, Roche).