sdw_72h replicate 3
The plant material was pulverized by grinding with liquid nitrogen and total RNA was isolated using the Direct-zol RNA Miniprep kit (Zymo Research, Nordic BioSite, Sweden) following the manufacturers instructions. RNA quality check was performed using a 2100 Bioanalyzer system (Agilent, Denmark) and samples with RIN higher than 8 were selected for RNAseq. RNA samples obtained from drupes from all the treatments collected 72 hpi were sequenced at Novogene (Cambridge, UK) with Illumina HiSeq 4000 and 150 pair end read length (PE), at 30 million of read depths. Illumina library was prepared according the Illumina Sequencing By Synthesis (SBS) Kit, following the on-line manual (https://www.illumina.com/documents/products/techspotlights/techspotlight_sequencing.pdf) and prompt loaded it into the flow cell of Illumina HiSeq 4000 (Illumina, Inc., San Diego, CA, USA). Data quality control revealed a Q30 [(Base count of Phred value > 30) / (Total base count)] average of 93% for a total of 98% of clean reads.