RNA-Seq of Sugarcane_bagasse_T2-60
Genomic DNA (50 ng) from each sample was PCR-amplified for the 16S rRNA V1V9 region (~1.5 kb), purified, and barcoded using standard NEB and AmPure bead protocols. The pooled libraries were sequenced on the Oxford Nanopore MinION platform (R9.4 flow cell). Raw reads were base-called, de-multiplexed, trimmed, and size-filtered (12001950 bp) for downstream microbiome analysis.