MNase-seq of S. cerevisiae derivative yGWG538: pZEVi-LYS2 induced (1000nM Beta-Estradiol), Rep 1
Chromatin from formalin-crosslinked yeast was digested with MNase for 60 minutes to generate predominantly mono-nucleosome DNA fragments within the 100-200bp range. After de-crosslinking, DNA was purified with a phenol-based method and then further purified with a column (Clean & Concentrator, Zymo). Sequencing libraries were prepared from purified DNA samples using the automated KAPA general DNA-seq kit to introduce Illumina adapters and dual-indexing for multiplexed, paired-end (51 x 2) sequencing that reads the ends of each DNA fragment.