GSM8945064: Bulk MPCs (Biol. replicate 3); Mus musculus; RNA-Seq
RNA for bulk cells was extracted using Qiagen's RNeasy mini kit according to the manufacturer's protocol. RNA for GFP+ sorted cells was extracted using Qiagen's Rneasy micro kit according to the manufacturer's protocol. Libraries constructed according to Illumina's TruSeq stranded mRNA protocol. In brief, total RNA was polyA-enriched and reverse-transcribed to cDNA. cDNA was fragmented, end-repaired, adenylated and ligated with TrueSeq adapters containing unique dual indices (UDI). Lastly, adapter-ligated cDNA was selectively amplified by PCR and sequenced on an Illumina Novaseq 6000 with single end reads. Prior to sequencing amplified cDNA was quality-controlled on a fragment analyzer.