GSM8944990: IF1-KO cells + GFP, seq run NOVO0082, rep2; Homo sapiens; RNA-Seq
Medium was removed and cells were gently washed once with ice-cold PBS. RNA was isolated using RNeasy® Mini Kit with on-column DNase digestion (Qiagen) according to the manufacturer's instruction. RNA was quantified using Nanodrop, and for quality control, samples were re-assessed using Qubit 3.0. RNA integrity was confirmed using Agilent RNA 6000 Nano Kit in 2100 Bioanalyzer (Agilent). Libraries were prepared using TruSeq Stranded mRNA kit (Illumina) according to the manufacturer's instruction.