GSM8578202: Testis_LH_m66; Solea senegalensis; RNA-Seq
Total RNA from was extracted using the RNeasy Plus Mini Kit (Qiagen). The extracted RNA's purity and concentration were assessed using the NanoDropVC 2000, and RNA size distribution profiles were analysed using the Agilent 2100 Bioanalyzer. RIN values were comprised between 8.7 and 9.8. For library construction (n = 4 for each group: control, rFsh and rLh), the SMARTseq2 protocol was employed, with some modifications. The libraries were sequenced on a NovaSeq 6000 S1 sequencing system (Illumina) in paired-end mode with 100 bp paired-end reads length.