GSM9577718: A498 ELOVL2 and ELOVL5 double knockdown replicate 1; Homo sapiens; RNA-Seq
Total RNA was extracted from A498 renal cancer cells after siRNA transfection. RNA quality and quantity were assessed prior to library preparation. RNA-seq libraries were prepared from total RNA obtained from A498 renal cancer cells transfected with control siRNA or siRNAs targeting ELOVL2, ELOVL5, or both genes. Libraries were validated using a Bioanalyzer (Agilent Technologies) to determine size distribution and concentration. Validated libraries were sequenced on an Illumina NextSeq 500 platform using the paired-end 36-base read option.