GSM8796041: Sham2; Rattus norvegicus; RNA-Seq
Nuclei were isolated following the Parse Evercode V2 kit protocol and published methods. T13-L1 bilateral DRGs were minced (<1mm) using Bonn scissors and mechanically dissociated with a Dounce homogenizer (15 strokes). The isolation buffer contained sucrose, KCl, MgCl₂, Tris (pH 8.0), DTT, protease inhibitor, Triton X-100, and RNAsin. The tissue was filtered through a 70 µm strainer, centrifuged, and the nuclei pellet was washed with a BSA/RNase inhibitor/DPBS buffer. Nuclei were counted using Trypan Blue and a hemocytometer, then fixed and processed following the Evercode Nuclei Fixation v2 kit. Library preparation followed the manufacturer's instructions. https://support.parsebiosciences.com/hc/en-us/categories/18950704717076-User-Guides