GSM9176673: Eosminus Treg Rep3; Mus musculus; RNA-Seq
RNA was extracted from sorted cells using TRIzol (Thermo Fisher Scientific) and RNeasy Plus Micro Kit (QIAGEN) according to the manufacturer's instruction. The SMARTer Ultra Low Input RNA Kit was used to generate high-quality cDNA directly from 1 ng of Total RNA using their patented SMART (Switching Mechanism at 5' End of RNA Template) technology. The cDNA is then made into a sequencing ready library using the Nextera® XT DNA Sample Preparation Kit from Illumina.