GSM8693341: HiC 72hr Mock Rep2; Homo sapiens; Hi-C
Cells were trypsinized, washed in PBS, and collected for Hi-C crosslinking and library prep. Approximately 1 million cells per Hi-C experiment were used. The Arima Hi-C kit (#A410231) was used to generate Hi-C libraries. Breifly, in-situ HiC was performed by fixing cells with PFA. Samples were sheared to 400pb using Covaris and made into sequencing libraries using the NEB Ultra II DNA Library Prep Kit (#E7645S). Libraries were sequenced 2x150bp.