RNA-Seq of Arabidopsis: SUC2 translatome -Pi1
The RNA was extracted from immunoprecipitation of ribosome complexes from pSUC2:HF-RPL18 line. The quality and integrity translatome were determined by Agilent 2100 Bioanalyzer with RNA 6000 Pico Kit chip (Agilent Genomics), only translatome with RNA integrity number greater than 8 were used for RNA library construction. Strand-specific RNA sequencing libraries were constructed using Illumina TruSeq Stranded mRNA Library Prep Kit following the protocol from the manufacture with several modifications to adapt to the low RNA input. The RNAs input were 100 ng for pSUC2:HF-RPL18  line.