GSM9142466: Chronic_MidleSI_Control_rep3; Mus musculus; RNA-Seq
RNA-seq: Total RNA was extracted from intestinal tissue using the RNeasy Mini Kit (Qiagen), following the manufacturer's instructions. scRNA-seq: Mouse colon tissues were fixed with formaldehyde and dissociated using gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec). Tissue processing was performed following the protocol "Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling" (CG000553 Rev.B, 10x Genomics). RNA-seq: RNA libraries for RNA-seq were prepared using the TruSeq Stranded mRNA Library Prep Kit (Illumina) following the manufacturer's instructions. scRNA-seq: Single-cell libraries were prepared using the Chromium Fixed RNA Profiling kit (10x Genomics) following the manufacturer's protocol (CG000527 Rev.D). Fixed cells were hybridized with probe sets, pooled, barcoded using Chromium X, and amplified with dual-indexed primers. Libraries were sequenced on an Illumina NovaSeq 6000.