Hi-C data of Aurelia coerulea (Aco2019JZB03)
For Hi-C library preparation, tissue from Aurelia coerulea was freeze-dried, powdered, and resuspended in nuclei isolation buffer. Nuclei were isolated and treated with 0.5% SDS at 62C for 10 min, then digested with MboI (NEB). DNA fragments were end-filled, biotinylated, and ligated using T4 DNA ligase. After purification, biotinylated fragments were captured with Dynabeads MyOne Streptavidin T1 (Invitrogen) and amplified. Libraries were sequenced on an Illumina NovaSeq 6000 platform in paired-end 150 bp layout.