GSM8488899: Osteal macrophages OVX--Sham_scRNA; Mus musculus; RNA-Seq
Isolated Omacs were washed once with ice-cold PBS containing 0.04% weight/volume BSA and counted using AO/PI staining by CellDropFL (DeNovix, Wilmington, DE). The cells were then subjected to cell hashing (TotalSeq™; BioLegend) and the resulting cells (1037 for OVX) and (1084 cells for sham) were loaded Library was perfomed according to the manufacturers' instructions (Chromium Next GEM Single Cell 3' Reagent Kits v3.1).