GSM9396964: ATAC_WT4; Mus musculus; ATAC-seq
50,000 Spg per sample were processed. After cell lysis, transposition was performed for 30 min at 37ºC using an in-house synthesized Tn5 transposase pre-loaded with sequencing adapters compatible with Illumina platform. Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina with custom primers (Integrated DNA Techologies, ATAC-Seq). Libraries were amplified for 12 PCR cycles followed by purification and pooling for sequencing.