Soil microbial community (Contaminated soil + ARBC-loaded Enterobacter mori XJ10, T3), Biological Replicate 2
Soil samples were collected from Shenzhen Basic Farmland Protection Area (22.602365 N, 113.919507 E), spiked with Cd(NO) and Pb(NO) to final concentrations of 5.18 mgkg (Cd) and 514 mgkg (Pb), and aged for 1 month. 5 treatments (3 biological replicates each): OS (original soil, no contamination), CK (contaminated soil, no amendment), T1 (contaminated soil + 5% (w/w) Atlantibacter hermannii XJ 08 suspension, 110 CFUg), T2 (contaminated soil + 5% (w/w) BS-AMBC, brown sugar-modified Astragalus membranaceus residue biochar), T3 (contaminated soil + 5% (w/w) BS-AMBC@A. hermannii XJ 08 composite). All samples were incubated at 252C for 90 days (soil moisture: 60% field capacity). Aim: Evaluate Cd-Pb immobilization and soil microbial community changes via 16S rRNA gene sequencing (Illumina NovaSeq, single-end).