replicate 2 of BG4 CUT&Tag in HeLa, untreated
CUT&Tag was constructed as described (Zheng et al., 2024). The bead-bound cells were resuspended in 100 uL dig-wash buffer (0.05% digitonin, 0.1% BSA, 20 mM HEPES-KOH at pH 7.5, 150 mM KCl, 0.5 mM spermidine, 2 mM EDTA, 1 uL protease inhibitors) containing 1/50 DNA G-quadruplex (BG4) antibody (MABE917, sigma) and followed by anti-FLAG M2 antibody (F1804, sigma) and secondary antibodies.