NanoSeq of homo sapiens: normal human urothelium no infection, undiluted controls
Ureteric urothelial cells were isolated from excess tissue after renal transplant. Cells were expanded then underwent biomimetic re-differentiation, forming a tight barrier. Cells were then exposed to maintained for 21 days when DNA was harvested. DNA was then processed according to the Sanger Institute (Cambridge, UK) NanoSeq pipeline.