GSM9001282: Healthy_Skin, rep13; Homo sapiens; RNA-Seq
Samples were thawed, and RNA extraction was carried out using Trizol along with the Qiagen RNAeasy kit (Qiagen, Germany), adhering to the manufacturer's instructions. For RNA sequencing, total RNA (100–1,000 ng per sample) underwent ribosomal RNA depletion, was reverse-transcribed into double-stranded cDNA, and then selectively enriched by polymerase chain reaction (PCR). The enriched libraries' quality and quantity were assessed using the Fragment Analyzer (Agilent, Santa Clara, California, USA). Libraries were then normalized to a concentration of 10 nM in Tris-Cl 10 mM, pH 8.5 with 0.1 % Tween 20. Sequencing and cluster generation were performed using the HiSeq2500 (Illumina, Inc, California, USA) following the standard protocol.