RNA-Seq Columbia0 HCol12 replicate 1
7-day-old seedlings were collected from Columbia WT or homozygous HCol12 mutant (35S::p500:ARM genomic construct agrofiltrated to Col-0). Total RNA was isolated using TRI reagent and integrity of RNA was checked by agarose gel electrophoresis, concentration was measured using a NanoDrop spectrophotometer. Libraries were prepared by random fragmentation of the cDNA, followed by 5 and 3 adapter ligation (TruSeq Stranded mRNA LT Sample Prep Kit). Adapter-ligated fragments were PCR amplified and gel purified. Fragments were sequenced on the Illumina NovaSeq 6000 Platform by paired-end read sequencing, generating 150 bp read length. Three biological replicates were analyzed