GSM9438767: WGBS, P8 testis, Wild type, biol rep1; Mus musculus; Bisulfite-Seq
After samples DNA testing, positive control DNAs were added into the DNAs, and then they were fragmented into 200-400bp using Covaris S220 (Covaris, USA). Next, ssDNA fragements were Bisulfite treated. After BS treatment, unmethylated Cytosine will change into Uracil, while methylated Cytosine will stay unchanged. Then, methylation sequencing adapters ligation, size selection and PCR amplification steps were performed to the DNA fragments. The library kit is Accel-NGS Methyl-Seq DNA Library Kit (Swift, USA, Catalog #:30096). Subsequently, library quality was assessed on the Agilent 5