GSM8765712: 2-HCT116_D350Atet_untreated_ChIP_p53; Homo sapiens; ChIP-Seq
Non-histone samples were first crosslinked with EGS for 25 minutes. All cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, cells were sonicated with a Cavaris S220 Ultrasonicator. Barcoded libraries were made using NEB Ultra II DNA Library Prep Kit for Illumina following manufacturer's instructions and quantified by Agilent 4200 Tapestation System.