V4-V5 region of 16S ribosomal RNA gene was amplified using 515f/806r primers from squirrel guts microbiomes:17-51cecmuc
The V4-V5 region of the 16S ribosomal RNA gene was amplified using 515f/806r primers from squirrel gut contents. Each primer combination had a unique barcode corresponding to a unique sample. Barcoded amplicons were normalized and pooled into a single library which was sequenced on a 2x150 Illumina MiSeq run. Sequences were subsequently demultiplexed by barcode into individual files corresponding to each sample.