CUT&RUN of mus musculus
The study aims to investigate the role of Mll4 in tooth enamel development by comparing gene expression profiles between Mll4 knockout (KO) and control (Ctrl) mice. Samples were collected from tooth germ tissue at birth (P0) to capture early ameloblast differentiation. RNA was extracted from these tissues and used to prepare RNA-seq libraries. Paired-end sequencing (PE150) was performed on the NovaSeq 6000 platform to generate high-quality transcriptome data. 4 biological replicates were included for each condition to ensure data robustness and reproducibility.