GSM8832621: DU145, CCS1477-2; Homo sapiens; RNA-Seq
Total RNA was extracted from DU145 cells using the RNeasy Mini Kit (QIAGEN, 74104). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification