AhR IP-2 (CRX1243301; CRA020103)
Genomic DNA was extracted from T. cinnabarinus.The Halo-TcAhR recombinant proteins was expressed according to the manufacturer's instructions for the TNT SP6-coupled wheat germ extraction system (Promega Inc., Fitchburg, USA). Magne HaloTag beads (Promega Inc., Fitchburg, USA) were used to purify the recombinant proteins, and Western blotting was used to confirm protein actually expression. Then, the DAP gDNA libraries were separately incubated with Halo-TcAhR recombinant proteins at room temperature for 1 hour. The eluted DNA was used for PCR amplification sequencing using Illumina HiSeq 4000 (Genetic Engineering Biotechnology Co. Ltd., Guangzhou, China)