16s rRNA sequencing of metagenome: human
After extraction with QIAamp Fast DNA Stool Mini Kit (Qiagen, Hilden, Germany), the V3-V4 hypervariable region of the 16S rRNA gene was amplified, followed by library preparation and sequencing through the Illumina MiSeq system (Illumina, CA, USA). Human read removal was performed with SRA Human Scrubber (filter version 20250916v335).