GSM8514929: MLC, IL-1β+LOXL2, Replicate2; Capra hircus; RNA-Seq
RNA was islated using Rneasy Mini kit (Qiagen). Around 400 nanogram of RNA was used for sequencing at NOVOGENE facility. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis.