GSM8606613: day 184 OVA+ cells, replicate 2, scRNAseq [VDJ_GEX]; Mus musculus; RNA-Seq
The OVA+ cells were enriched by OVA-PE tetramer-pulldown. Splenic CD4+ T cells were enriched by CD4 magnetic beads(MIltenyi). mRNA was extracted from lung single cell suspension using the barcoded Single Cell VDJ 5' Gel Beads onto 10X Chromium Next GEM Chip K. Library construction was performed according to 10x Genomics Chromium™ Single Cell 5' VDJ v2 enrichment and gene expression. single cell 5' gene expression and single cell V(D)J dual index libraries