GSM8607282: Crosslinked MCF10A, PUF60 IN, rep2; Homo sapiens; RIP-Seq
Cells were lysed and and sonicated in eCLIP lysis buffer, and RNA was fragmented using RNase I (Ambion) and complexes were immunoprecipitated with protein-specific antibodies from Bethyl Laboratories RNA was reverse transcripted with Superscript III, and adaptor was ligated with T4 RNA Ligase 1, then libraries were constructed with Q5 High-Fidelity 2x Master Mix (NEB)