16S rRNA from mock community
two-step PCR protocol based on NEXTERA technology, incorporating IDT for Illumina NEXTERA DNA Unique Dual Indexes. The V4 region of the 16S rRNA gene was amplified using the primer pair 515F and 806R. . Library quality control (QC) was conducted using the Agilent Bioanalyzer 2100 with the High Sensitivity DNA Kit. Library quantification was performed using the Invitrogen Collibri Library Quantification Kit and the Quant-iT 1X dsDNA High-Sensitivity Assay Kit on a Qubit fluorometer. Following QC and quantification, libraries were pooled in equimolar concentrations.