GSM8832549: V24A_rSt_mut_rep1; Mus musculus; RNA-Seq
FACS-isolated populations of ScLZ, ScPD, rSt and elSt cells were pelleted at 2000g for 10 min at 4°C. Total RNA was extracted from cell pellets using the RNeasy Mini Kit (Qiagen 74104) according to manufacturer's instructions. DNA removal was performed using on-column the RNase-Free DNase Set (Qiagen, 79254). RNA concentration was determined with Qubit RNA HS Assay Kit (Q32852). Quality of RNA was determined using Fragment Analyzer system Libraries were prepared with the Illumina Stranded total RNA-seq protocol according to manufacturer's instructions. Quality control of libraries was performed with Agilent 2100 Bioanalyzer System.  After passing the QC, sequencing was performed on Illumina NovaSeq platform (50 bp, paired end).