Mitogenome sequencing of homo sapiens : control for Parkinson's disease
The mitogenome was then amplified through polymerase chain reaction (PCR) using 33 pairs of carefully designed primers, described by Cavalcante et al (2019). Library preparation for mitochondrial genome sequencing utilized the Illumina Nextera XT DNA kit (Illumina Inc., Chicago, IL, USA), following the manufacturers instructions. DNA quality was verified with the high-sensitivity ScreenTape D1000 and in the Agilent 2200 TapeStation system. The actual sequencing took place on the Illumina MiSeq system, using the MiSeq Reagent Kit v3 (600 cycles) from Illumina.