GSM9113399: strain YJ-09, high SAM level, 36h, rep2; Saccharomyces cerevisiae; RNA-Seq
Frozen samples were cryogenically grinded into powder, and total RNA was extracted using the RNAprep Pure Plant Plus Kit (TIANGEN, China). Agilent 2100 bioanalyzer (Agilent Technologies, CA, USA) was used to detect RNA integrity and total amount accurately. Fast RNA-seq Lib Prep Kit V2 (Cat.No.RK20306) was used to prepare library for transcriptome sequencing. After the library construction, Qubit 2.0 Fluorometer was used for preliminary quantification. The insert size was detected using Agilent 2100 bioanalyzer. Then the effective concentration of the library was quantified using qRT-PCR. The effective concentration of the library need higher than 1.5 nM.