Genome skimming: Astragalus flexuosus var. flexuosus - JLMC 9674
Fragmentation with Bioruptor Pico sonicator using 7 cycles of 15 s On/90 s Off; NEBNext Ultra II DNA Library Prep Kit with no size selection and half volumes, 6 PCR cycles using NEBNext Dual Index Primers i704 and i503; Index Read 1: GAGATTCC, Index Read 2: AGGATAGG