GSM9065340: SNU-449 cells, nucleus,NC,rep1; Homo sapiens; RNA-Seq
RNA was isolated from the cytoplasmic and nuclear extracts using Trizol reagent(Invitrogen). RNA quality was assessed with a NanoDrop ND-1000 spectrophotometer. Directional RNA-seq libraries were constructed and then sequenced at the Anoroad Genome (Beijing, China) on an Illumina NovaSeq 6000 Platform.