16S rRNA: CZ_0220
DNA was extracted using the Qiagen DNeasy Mini Kit with an added bead-beating step to improve lysis efficiency. The V4 region of the 16S rRNA gene was amplified using primers 515F and 805R with dual barcodes. PCR products were purified, quantified, pooled in equal concentrations, and sequenced on an Illumina MiSeq (2250 bp) platform at Genewiz.