GSM9213154: NSC, ATAC, cKO, 1; Mus musculus; ATAC-seq
Nuclei isolated from 5×104 neural stem cells were used for ATAC-seq. Cells were harvested at 500 g 4°C for 5 min, and then cell pellets were re-suspended in 100 μL of lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.5% NP-40) and then nuclei were centrifuged at 500 g 4°C for 15 min. The nuclei pellets were re-suspended in the transposition reaction mix containing Tn5 transposase (Vazyme Biotech, Nanjing, China). The library was sequenced on an Illumina HiSeq X Ten sequencer.