GSM9472197: BJ fibroblasts, replicate 2, siFH, Day 5; Homo sapiens; RNA-Seq
RNA extraction was performed using the Maxwell® 16 LEV kit (Promega AS1270). RNA quality was evaluated with the Agilent 2100 Bioanalyzer system and 2100 Expert software. RNA integrity numbers for all samples were above 8. Libraries were prepared following the Stranded mRNA Prep protocol from Illumina, starting from 250 ng of high quality total RNA. Paired end (2 × 51 bp) sequencing was performed on an Illumina NovaSeq platform.