16S amplicon sequencing California Costal Environment
cDNA libraries were synthesized from the extracted RNA with SuperScript III First-Strand Synthesis System (Invitrogen). rRNA amplicon libraries targeting the 16S V4V5 and 18S V4 regions were prepared as described Amplicon Library Preparation using primer sets 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT-3) for 16S [52], and 18SV4 primer sets V4F (5-CCA GCA SCY GCG GTA ATT CC-3) [53] and a reverse primer V4RB (5-ACT TTC GTT CTT GAT YRR-3) [54]. The libraries (16S V4V5 and 18S V4) were sequenced on the MiSeq in PE300 mode (Illumina).