GSM8692516: RA-FLS 2; Homo sapiens; OTHER
CUT&Tag assay was conducted using Hieff NGS G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina (12598, Yeasen, China), following the manufacturer's instruction (three independent samples of each group). Briefly, cells were harvested and bound to concanavalin A-coated magnetic beads, reacted with primary antibody against H3K9la, secondary antibody of anti-rabbit IgG, and pA/G-Transposome Mix to activate transposase. FLSs were lysed by proteinase K and DNA was extracted, amplified and purified for library construction using Hieff NGS Tagment Index Kit (12416, Yeasen, China) The library construction using Hieff NGS Tagment Index Kit (12416, Yeasen, China),