RNA-seq of capsicum annuum: color-transition 3 stage pericarp
After the sample testing is qualified, the library construction is carried out with the following main steps: (1) Enrich eukaryotic mRNA using magnetic beads containing Oligo(dT); (2) Add Fragmentation Buffer to randomly break down the mRNA; (3) Use mRNA as a template to synthesize the first cDNA strand and the second strand, followed by cDNA purification; (4) The purified double-stranded cDNA undergoes end repair, A-tailing, adapter ligation, and size selection using AMPure XP beads; (5) Finally, the cDNA library is obtained through PCR enrichment.