GSM9031921: Invivo_W7_rep1; Mus musculus; Bisulfite-Seq
The genomic DNA was fragmented by sonication, followed by end-repair, adenine tailing, and adapter ligation. According to the manufacturer's instructions, the genomic DNA was subjected to bisulfite treatment using the EZ DNA Methylation Kit (D5001; Zymo Research), and subsequent PCR amplification was carried out.