GSM8944778: WT _Induced_Replicate_1; Saccharomyces cerevisiae; RNA-Seq
Total RNA isolation was carried out using Hot-Phenol Method followed by DNAse1 digestion and RNA clean up using  RNA clean up using RNA Clean and Concentrator kit . Prior to library preparation For spike in normalization of the RNA, an equal amount (2.4 l of 1:100 fold dilution) of ERCC spike-in mix (Thermo Fisher #4456740) was added to 1.2 ug of RNA samples of each strain. RNA-sequencing libraries preparation was performed by NHLBI-genome sequencing core using the Illumina TrueSeq stranded mRNA Library Prep Kit. Ribo-depletion was performed to remove the rRNA using Qiagen FastSelect Yeast rRNA Depletion Kit, followed by paired-end sequencing using the Novaseq-X platform.