GSM9616492: Planktonic, AF293, Hypoxia, rep4; Aspergillus fumigatus; RNA-Seq
Tissue was harvested in TRI reagent with bead beating, RNA was purified from the aqueous phase with Qiagen RNAeasy Plus Mini kit. DNA contamination was removed with the TURBO DNA-free kit (Ambion). 100 or 200 ng of RNA was used for library construction. RNA libraries for RNAseq were prepared using the mRNA HyperPrep kit (Kapa Bioscience) following manufacturer's instructions.