GSM9321009: IMOK infected with E. coli (no T-cell coculture) – replicate 1; Mus musculus; RNA-Seq
Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Cells were lysed directly in TRIzol, followed by phase separation with chloroform and centrifugation. The aqueous phase containing RNA was collected, and RNA was precipitated with isopropanol, washed with 75% ethanol, and resuspended in RNase-free water. RNA concentration and purity were measured using a NanoDrop spectrophotometer, and RNA integrity was assessed by TapeStation. RNA-seq libraries were generated using the 3′ mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices (12 nt) following the manufacturer's instructions. Libraries were indexed and amplified according to kit guidelines.