GSM8898468: Hela NDF-WT, Pol II, ChIP_DRB_20min; Homo sapiens; ChIP-Seq
For ChIP-seq, cells were crosslinked with 1% formaldehyde (Sigma) for 10 min, after quenching and lysis, chromatin was sonicated with a Covaris S220 focused-ultrasonicator. ChIP-seq libraries were prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB E7645S) according to kit instructions.