GSM8635764: A549-shNRF2-WT NRF2_rep1; Homo sapiens; RNA-Seq
RNA isolation was performed using the RNeasy Plus Mini Kit (Qiagen) and reverse transcription was performed using the High-Capacity cDNA Reverse Transcription Kit (Thermo) according to the manufacturer's protocol. the Truseq Stranded mRNA kit (Illumina) was used with an input of 100-1000 nanograms of total RNA. Libraries were quantified with Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and the average library size was determined using D1000 ScreenTape on TapeStation 4200 (Agilent Technologies).