ddRADSeq of Skiffia francesae: adult fin clip tissue
Whole genomic DNA from fin clips was extracted with a Qiagen DNEasy kit. Library prep followed the Peterson et al. 2012 protocol and used PstI and MspI restriction enzymes. Qiagen QIAquick PCR Purification Cleanup kit use to purify sequences prior to pooling. Pooled sequences were size-selected on a BluePippin for 350-600bp prior to being sequenced for 100bp single-end reads on an Illumina HighSeq 4000.