GSM9689720: spleen, Control, short-term,3; Mus musculus; RNA-Seq
Tissues were collected and immediately submerged in stayRNA™ (A&A Biotechnology) reagent. After 24 of cooling (4C), tissues were frozen at -80C until the time of RNA isolation. For RNA isolation, tissue scrapes (10-20 mg) were homogenized and used for RNA isolation with AllPrep DNA/RNA (Qiagen) kit. Following isolation, RNA was controlled for quality with the TapeStation4150 system and quantified. Libraries were prepared using QuantSeq 3'mRNA-Seq (FWD) V2  (Lexogen) following standard protocol.