GSM8636639: HEK_ms_IgG_rep1; Homo sapiens; OTHER
~500K HEK293T cells per sample were detached from plates using trypsin and immediately washed 2X in CUT&RUN wash buffer according to EpiCypher CUTANA CUT&RUN protocol v2.0. CUT&RUN was performed with mouse anti-FLAG (Sigma F3165), rabbit anti-myc (CST 13987), rabbit IgG (Sino Bio CR1) and mouse IgG (sc-2025) antibodies. CUT&RUN libraries were generated using the EpiCypher CUTANA CUT&RUN Library Prep Kit v1.5.