GSM9080228: JM747 RNA-seq; Mus musculus; RNA-Seq
Total RNA was isolated with the  Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Kit . The RNA was DNAse I treated to eliminate genomic DNA, then the purified RNA was utilized to make libraries for next generation sequencing. Bulk RNA Libraries were constructed following standard Illumina protocols for RNA sequencing in the University of Texas MD Anderson Cancer Center Advanced Technology Genomics Core (ATGC). Indexed cDNA libraries were minimally PCR amplified, pooled and sequenced.