A12S-4 (CRX1819267; CRA027274)
Total microbial genomic DNA was extracted from midguts using TIANamp Genomic DNA Kit (TIANGEN Biotech, Cat# DP304-02). The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F and 806R by an T100 thermal cycle (BIO-RAD, USA). The PCR product was extracted from 2% agarose gel and purified. Purified amplicons were pooled in equimolar amounts and paired- end sequenced on an illumine NextSeq 2000 PE300 platform according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd (Shanghai, China). submitted_instrument_model: Illumina NextSeq 2000