GSM8489631: Splenic cDC1, Itgax-Uqcfqf/f, rep3; Mus musculus; ATAC-seq
5 x 104 cDC1s or cDC2s were lysed by spinning at 500 x g for 20 minutes at 4°C in cold lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM MgCl2, 0.1% IGEPAL CA-630). Nuclei pellets were resuspended in the transposase reaction mix (including 2 ml Transposase (Illumina)) and the transposition reaction was carried out for 1 h at 37 ºC. Then, samples were purified using PCR Minelute kit (Qiagen) following the manufacturer's instruction. Libraries were generated by PCR using the NEBNext High-Fidelity PCR Master Mix (New England Biolabs) and the following PCR conditions: 72°C for 5 minutes, 98°C for 30 seconds, followed by thermocycling at 98°C for 10 seconds, 63°C for 30 seconds and 72°C for 1 minute for 12 cycles. PCR product was purified using PCR Minelute kit (Qiagen) and SPRI beads-based size selection carried out using AMPure XP (Beckman). Libraries size and quality were assessed using a 2100 Bioanalyzer instrument (Agilent).