GSM8606424: B cell, aCD40 + IL-4 + IL-21, 6hr, rep2; Mus musculus; RNA-Seq
Total RNA was extracted using Zymo RNA miniprep kit (ZymoResearch, R1054) following the manufacturer's protocols. 1 μg of total RNA was used for polyA enrichment using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490L) Libraries were constructed using Poly(A)-enriched RNA samples by xGEN Broad-Range RNA Library Preparation kit (IDT, 10009865) and xGEN UDI Primers Plate 2 (IDT, 10009816)