GSM9033119: orb2-delta-zz  2-3h rep1_pool3; Drosophila melanogaster; RNA-Seq
The polysome gradient protocol was adapted from Chen et al., 2014, with modifications. See paper MATERIALS AND METHODS. RNA libraries were prepared using a NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina and following the manufacturer's protocol. Ribosomal RNA depletion was performed using the QIAseq FastSelect rRNA Fly Kit (QIAGEN, Cat# 333265 (96 rxns) following the manufacturer's protocol.