Isolation, characterization and functionality exploration of Klebsiellapneumoniae specific bacteriophage isolated from hot and humid tropicalregion
Genomic DNA was extracted from the received Bacteriophage lysate sample using modified QIAGEN Blood DNA Extraction kit as per manufacturer's instruction. The quality and quantity of the extracted DNA sample was checked on NanoDrop by determining A260/280 ratio and followed by agarose gel electrophoresis.DNA Seq library was prepared by Illumina Truseq DNA library preparation kit. Library validation will be carried out using High Sensitivity D1000 Screen tape using Agilent TapeStation and sequencing of library on Illumina Platform.