GSM8515882: lazy_piggy_restriction_splink_insertions_lane5; Mus musculus; OTHER
Snap frozen tumour tissues were digested with proteinase K and gDNA extracted by phenol/chloroform extraction followed by ethanol precipitation. Extracted gDNA were mechanically sheared to 300 bp fragments using a Covaris S220 sonicator. End repair was performed using an EpiCentre End-It Kit, followed by adaptor ligation. With splinkerette-adaptors ligated at both ends, BamHI was used to digest and remove gDNA fragments. Primary and secondary PCR amplifications were performed using primers specific to sites specific to either the SB transposon sequence, PB transposon sequence, or their boundary, for amplification of IR, PB, and JX libraries, respectively.