RNA-seq of Ciona intestinalis: whole embryos at St. 21 in DMSO-treated control condition (batch 4)
Total RNA from whole embryos of Ciona intestinalis were extracted using the NucleoSpin RNA kit (Macherey-Nagel). Purified RNA quantity and quality was assessed using a Bioanalyzer (Agilent Technologies, USA). Library construction was performed using NEBNext ultraII directionnal RNA library prep kit (New England Biolabs) and single-end sequencing was performed using NextSeq550 (Illumina).