GSM8693336: NHDF, MOI5 96hr, H3K9me3, Rep2; Homo sapiens; OTHER
Cells were dislodged from plate using 0.05% trypsin and resuspending in 5% FBS media for further processing. Approximately 500,000 cells per CUT&RUN experiment were used. Libraries were made following Epicypher's CUTANA CUT&RUN Library Prep kit ( (#14-1048) including E.coli spike-in controls. Antibodies used were CST Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb #9751, Di-Methyl-Histone H3 (Lys9) (D85B4) XP Rabbit mAb #4658, and Epicypher kit provided IgG control. Libraries were sequenced 2x150bp.