GSM9578699: Homo sapiens, WTAP KD_IP_3; Homo sapiens; OTHER
Total RNA was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. RNA concentration and purity were assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific, USA). Polyadenylated mRNA was subsequently purified from total RNA using the GenElute mRNA Miniprep Kit (Sigma, cat. no. MRN10-1KT) through two rounds of enrichment. the polyA RNA was fragmented at 86 °C for 7 minutes using the Magnesium RNA Fragmentation Module (NEB, cat. no. E6150), then incubated for 2 hours at 4 °C with 5 μg of m6A-specific antibody (Synaptic Systems, cat. no. 202003) in immunoprecipitation buffer (50 mM Tris-HCl, 750 mM NaCl, 0.5% Igepal CA-630). Immunoprecipitated RNA  were extracted, purified, and subjected to reverse transcription to generate cDNA.