GSM8832306: E. coli RS218 in Serum-replicate 2; Escherichia coli; RNA-Seq
Total RNA was extracted using the RiboPure RNA Purification Kit (Invitrogen, Wal-tham, MA, USA) and was further processed for rRNA depletion to enrich for bacterial mRNA using the MICROBExpress Bacterial mRNA Enrichment Kit (Invitrogen, Waltham, MA, USA). RNA libraries were prepared using the TruSeq® Stranded mRNA Library Kit (Illumina Inc., San Diego, CA, USA). Enriched mRNA was fragmented and primed for synthesis of first-strand and second-strand complementary DNA (cDNA). The resulting double-stranded cDNA fragments were adenylated at the 3' ends, ligated to multiple index adapters (TruSeq RNA Combinatorial Dual Indexes; Illumina Inc., San Diego, CA, USA), and enriched through PCR amplification.