scTCRseq of unstimulated human PBMCs
scRNA library preparations were performed using the 10x Genomics Chromium™ Single Cell system and Chromium™ Single Cell 5′ Library & Gel Bead Kit v2 (10x Genomics-PN1000263). TCRs were enriched using the TCR Amplification kit (10x Genomics-PN1000252), following the manufacturer's instructions. Initial cell input for each sample was 20,000 PBMCs to recover a total of 10,000 cells. Sequencing was performed on the NovaSeq platform (Illumina) using 10x Genomics recommended features and with a depth of 50,000 reads per cell