GSM9064840: adult brain ATAC-seq, mor-RNAi CT0 repeat-3; Drosophila melanogaster; ATAC-seq
Flies of genotype ClkGAL4>UAS-tetR-GFP-NLS was used for a bright GFP marker specific to clock neurons and DAPI was used as a viability marker. A small population of cells were heated at 60C for 5min and used as a dead cell (DAPI+) control. Live clock neurons are defined by GFP+ and DAPI- gating. We followed previously published ATACseq protocol for fruit fly (Yuan et al. PLoS Genetics 2024, doi:  10.1371/journal.pgen.1011278). We followed previously published ATACseq protocol for fruit fly (Yuan et al. PLoS Genetics 2024, doi:  10.1371/journal.pgen.1011278).