GSM9321143: flox_ctrl; Mus musculus; RNA-Seq
After a 2-week period, mice were humanely sacrificed, and their femurs were extracted. Bone marrow isolation via Dulbecco's Modified Eagle's Medium (DMEM) flushing yielded single-cell suspensions for downstream scRNA-seq analysis. The Chromium Single Cell 3' Reagent Kit of the Chromium platform (10X Genomics, Pleasanton, CA) was used for sequencing in reference to the manual. The library preparation was carried out and the sequencing was executed on the Illumina NextSeq 500 system (Illumina, San Diego, CA, USA).