Ovary_F2_WGBS_Con3 (CRX1817730; CRA027217)
DNA of offspring ovaries was extracted from the tissues using a Hi-DNAsecure Plant Kit, sonicated to 200-500 bp fragments using a sonicator. The conversion of unmethylated cytosines to thymidines of 1microg fragmented DNA was carried out using the EZ DNA Methylation-Gold Kit (Zymo, D5005). Libraries were prepared with the Accel-NGS Methyl-Seq DNA Library Kit (Swift, 30024).