GSM9032022: LSEC SW-CSCI rep 1; Homo sapiens; RNA-Seq
RNA was harversted using mini plus kit (Qiagen). Roughly 200ng of high quality total RNA was used for the transcriptomic assays. Library construction was performed following Ilumina recommendation. Sequencing library was prepared by random fragmentation of the cDNA sample, followed by 5' and 3' adapter ligation. The fragmentation and ligation reactions were performed in a single step that increases the efficiency of the library preparation process.  Then, adapter-ligated fragments were amplified by PCR and purified in a gel. Subsequently, library was loaded into a flow cell where fragments were captured on a lawn of surface-bound oligos complementary to the library adapters.