GSM8694864: CD45+ cells, Sham; Mus musculus; RNA-Seq
Immune cells were isolated from infarcted hearts of sham- or LAD-operated Pcsk9+/+ and LAD-operated Pcsk9-/- mice and washed with MACS buffer. CD45+ immune cells were selectively isolated using CD45 magnetic microbeads, MACS columns, and a separator. Libraries were constructed from collected single-cell suspensions, which were then subjected to 3' single-cell RNA sequencing, using Single Cell A Chip Kit, Single Cell 3' Library and Gel Bead Kit V2 and i7 Multiplex Kit (all from 10x Genomics) with a cell recovery target of 10000, following the manufacturer's instructions.