GSM9066321: Male donor 3, M0 macrophage, 50ng/mL M-CSF; Homo sapiens; RNA-Seq
Cultured cells were lysed in RLT buffer (Qiagen) and RNA was extracted using the RNeasy mini kit (Qiagen). RNA-seq libraries were generated using SMART-Seq2 protocol (Trombetta et al., 2014). Briefly, cDNA was reversed transcribed from single cells using Superscript III (Thermo Fisher Scientific) and whole transcriptome amplification (WTA) was performed. WTA products were purified using the Agencourt AMPure XP beads (Beckman Coulter, IN, USA) and used to prepare paired-end libraries with Nextera XT  (Illumina, CA, USA).