GSM8516553: Top1-cKO_T-cells_Rep3; Mus musculus; RNA-Seq
EpCAM+CD45-MHCIIhiLy51hi cTEChi and EpCAM+CD45-MHCIIhiLy51lo mTEChi cells were sorted from 2-weeks-old mice and TCRb+CD4+ T cells were sorted from spleen of 7-weeks-old mice. Total RNA was isolated using TRI reagent. RNA concentration and integrity was assessed using Tapestation. ~2ng of RNA was used to prepare RNA-seq library using Takara SMARTer® Stranded Total RNA-Seq Kit v2 according to manufacturer's instructions.