GSM8606909: K562 P1-KD polysomes rep2; Homo sapiens; OTHER
(Sample 13~20) For Polysome-seq: 450 µl lysate was prepared as described (Analysis of mRNA Translation by Polysome Profiling. Methods Mol Biol 2404, 69-81, 2022.). 100 µl lysate was used to prepare input (shCtrl, PABPC1 KD). 350 µl lysate was used to prepare heveay polysomes (shCtrl, PABPC1 KD). Total RNA of input, polysomes were harvested using LS Trizol reagent respectively, dissolved using equal volume of nuclease free water, ERCC per component and removed rRNA in them. RNA libraries were prepared for sequencing using standard Illumina protocols.