GSM9316939: Liver, Mdr2KO, GeoMx, 5853; Mus musculus; OTHER
Nuclei extraction was performed by Singulomics according to their protocols. For GeoMx WTA, sample preparation and extraction were performed following the manufacturer's protocol following the RNA Slide Preparation Protocol (FFPE) in "GeoMx DSP Manual Slide Preparation User Manual" (MAN-10150-07). For snRNAseq data, Singulomics constructed 3' single cell gene expression libraries (Next GEM v3.1) using the 10x Genomics Chromium system. For GeoMx WTA, libraries were consturcted following their protocols "GeoMx DSP NGS Readout User Manual" (MAN-10153-08). Libraries were sequenced with P2-100 cycles on NextSeq 2000.