D39V_deless_noIPTG_1499
An IPTG-inducible S. pneumoniae D39V CRISPRi library was grown in vitro in an ess deleted genetic background and treated without IPTG, after which bacteria were collected followed by genomic DNA isolation, amplicon library construction and sequencing as described in https://pubmed.ncbi.nlm.nih.gov/34997243/