Metabarcoding of Arbuscular Mycorrhizal Fungi: Associated with Elymus canadensis replicate, 4
Sample comes from mesocosm with a single species of host plant. Sterile soil was inoculated with seven cultured species of arbuscular mycorrhizal fungi: Claroideoglomus lamellosum, Claroideoglomus claroideum, Entrophospora infrequens, Funneliformis mosseae, Racocetra fulgida, Cetraspora pellucida, Acaulospora spinosa. After a summer growing season, whole soil was homogenized, and DNA was extracted. Ribosomal LSU primers were used during PCR amplification.