GSM9144081: EV_270; Canis lupus familiaris; RNA-Seq
Prefrontal cortex RNA was isolated using the Zymo Direct-zol RNA miniprep kit (Zymo). EV RNA was isolated using the Exosomal RNA Isolation Kit (Norgen Biotek Corp.) Polyadenylated RNA libraries were generated for prefrontal cortex RNA (Tecan), whereas total RNA libraries were generated for EV RNA (Zymo-Seq RiboFree), both according the manufacturers' protocols. All libraries were sequenced on an Illumina NovaSeq 6000 platform to generate >40M 150 bp paired-end reads per sample.