GSM8657663: 34639_W3_S55; Mus; RNA-Seq
Ncr1+/+ or Ncr1gfp/gfp ILC1s were sorted from the liver of mice using BD FACSAriaTM Fusion. Total RNA was isolated from ILC1s using a miRNeasy Mini Kit (QIAGEN). SMART-Seq® Ultra Low Input RNA Kit for Sequencing–v4 (Takara Bio) was used for getting double-strand cDNA from each sample with 2 ng of input total RNA. The resulting cDNA was sheared using a Covaris LE220 sonicator. The sheared DNA was used for to prepare a sequencing library, using a KAPA HyperPrep Kit. The final libraries were quantified using the Qubit Assay Kit (Thermo Fisher Scientific) and Bioanalyzer (Agilent). Sequencing was performed using the single-read mode of 51 cycles of read1 and 7 cycles of index read with V4 reagents on a Hiseq 2500 system (Illumina). Real-time analysis (RTA) 2.2.38 software was used to process the image analysis and base calling.