GSM9063490: C2C12 cells, Day 7 myotubes Rep2; Mus musculus; RNA-Seq
RNA was extracted Zymo Direct-zol RNA Miniprep Plus kit according to manufacturer's instructions. Modifications included all centrifugation steps at 20,817 x g and an additional spin step before elution without buffer to elute residual wash buffer. RNA libraries were prepared using TruSeq® Stranded mRNA Library Prep kit (Illumina, # 20020595) according to manufacturer's protocol. 1µg of RNA was used per sample.