GSM9578629: Gt time(dpi):4 rep:A; Gaeumannomyces tritici; RNA-Seq
Samples were ground to a fine powder using a pre-cooled sterile mortar and pestle under liquid nitrogen. Samples were not allowed to thaw prior to RNA extraction. When the amount of tissue was limited (as in chapter 5), samples were ground in an RNAse-free 1.5 ml Eppendorf tube containing liquid nitrogen, using a pre-cooled RNAse-free disposable pestle (Thermo Scientific™, USA). RNA extractions were carried out using the E.Z.N.A.® Plant RNA Kit with the associated RNase-free DNase I Set (Omega-Biotek, USA) following the standard protocol provided. RNA was eluted in 30 μl RNAse free water and stored at -80 °C. mRNA library preparation was carried out by Novogene (China) using the Novogene RNA Library Prep Set (PT042) for polyA enrichment.