GSM9176923: RNA-Seq, Ma10 cells, Wildtype 1; Mus musculus; RNA-Seq
Total RNA was extracted from cells using Trizol method. RNA was further cleaned up by RNA clean up kit (Qiagen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP. It was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.