GSM9033039: D7+11 samples treated with FGF10; Homo sapiens; RNA-Seq
Suspension kidney organoids at D7+5 or D7+11 were transferred into a 15ml falcon for dissociation in 4ml of 1:1 TrypLE/Accutase solution (STEMCELL Technologies) at 37°C with occasional agitation (flicking) until large clumps were no longer visible. If the organoids were not dissociated after 20 minutes, mechanical dissociation was undertaken. The TrypLE/Accutase solution was quenched using an equal amount of TeSR-E6 and cells were run through a pre-wet 40µm cell strainer to make them into single cell suspension. Library was constructed according to manufacturer's protocol, 10x Chromium Single Cell Expression Flex Protocol (CG00527, Revision E).