Wild-type Arabidopsis thaliana Seed (F1 Generation, 1.5 mg-C/L GO Treatment, Replicate 2)
Genomic DNA was extracted from approximately 30 mg of seedsDNA was fragmented to 200300 bp using a Covaris sonicator and purified with the Agencourt AMPure XP-Medium Kit. After end repair, A-tailing, and adaptor ligation using the BGI Plug-In Adapter Kit, PCR amplification was performed on a Bio-Rad thermal cycler. The library quality was assessed using the Agilent 2100 Bioanalyzer with the DNA 1000 Reagents. Paired-end 150 bp sequencing was performed on the DNBSEQ-T7RS platform (MGI), generating high-quality sequencing data for downstream analysis.