Ye-2 (CRX1506494; CRA022783)
RNA was extracted using a polysaccharide and polyphenol plant total RNA extraction kit (each sample was guaranteed to be approximately 100 mg), and the extraction process was carried out according to the instructions. After RNA extraction, a Nanodrop was used to determine the RNA purity (OD260/280) and concentration and to determine whether the nucleic acid absorption peak was normal. The integrity of the RNA was accurately detected with an Agilent 2100 instrument. After the samples passed the test, Sequencing was performed using the Illumina HiSeq 2500 sequencing platform.