GSM8693277: NHDF, Mock, siControl-A, 72hr Rep1; Homo sapiens; OTHER
Following labeling, cells were lysed with TRIzol and RNA phenol-chloroform extracted. Total RNA was fragmented using the NEBNext RNA Fragmentation Module and labelled with MTSEA-biotin-XX. 4sU labeled RNA was enriched wirth Streptavidin beads. The NEBNext rRNA Depletion Kit v2 was used prior to library prperation. Libraries were generated using NEB Ultra II Directional library prep kit (#E7760).  Libraries were sequenced 2x150bp.