GSM8578289: left ventricle, MI, 7d, input rep 3; Mus musculus; ChIP-Seq
Minced tissue was homogenized using a mechanical tissue homogenizer, then cross-linked with 1% formaldehyde for 10 min at room temperature and then quenched with 125 mM glycine. ChIP and input DNA fragments were end-repaired and A-tailed using the NEBNext End Repair/dA-Tailing Module followed by adaptor ligation with the NEBNext Ultra Ligation Module. The DNA libraries were amplified for 15 cycles and sequenced using Illumina Hi-Seq with paired-end 2x150 as the sequencing mode.