WGA of Borreliella afzelii: adult female tick (Ixodes ricinus)
All DNA samples (direct extraction from positive controls and WGA from both controls and fresh tick samples) were subjected to DNA quantification using Qubit (dsDNA Quantification Assay, Broad-range) and followed by sample processing for both Nanopore and Illumina sequencing. Standard Library Preparation was performed for Illumina sequencing using Nextera XT following manufacturer instructions (final concentration of each sample was 0.2 ng/l). Libraries were then sequenced in house on a MiSeq machine and v2 (500 cycles) reagents. In parallel, aliquots of the same samples were subjected to Nanopore sequencing (Nanopore sequencing was performed by the company Eurofins).