RNA-Seq of E. caballus
Purified cDNA samples were quantified by Qubit DNA High Sensitivity kit (Thermo Fisher Scientific) and each sample was employed for sequencing libraries using the Nextera XT DNA Library Prep kit (Illumina) according to the manufacturers instructions. The P5 and P7 indexes and adapters were incorporated into the 500 bp amplicons by the overhang adapters added to the primers. Qubit DNA High Sensitivity kit (Thermo Fisher Scientific) was used to verify the library concentration and the quality of amplicons was confirmed by Bioanalyzer High Sensitivity DNA Analysis (Agilent). PBMC-derived amplicon libraries were paired-end (2x300) sequenced using Illumina MiSeq