GSM8970977: Macrophage, 14P TAM, rep 4; Homo sapiens; RNA-Seq
5 x 105 PBMC-derived Macrophages were plated in a 6-well plate. Afterwards, they were either maintained as resting, M0, or polarized towards M1, M2, JX14P-TAM, or JX14P-RT TAM states. Total RNA was extracted using a Qiagen RNeasy mini kit (#74104, Qiagen, Netherlands). Samples were submitted to Novogene (Novogene Co., Ltd., Beijing, China) for DNase digestion, quality control assessment, library preparation, and sequencing. RNA Integrity was assessed using RNA ScreenTape (Agilent Technologies TapeStation), with all RNA integrity numbers greater than 9.0. Ribosomal RNA was depleted before conducting stranded library preparation.