GSM9112274: Healthy control 7; Homo sapiens; RNA-Seq
Total RNA was extracted from 1.0-2.0 × 10^6 PBMCs dissolved in 1.0 mL of Isogen reagent. Purified total RNA (200 ng) was used for RNA library preparation, following the Quant-Seq 3′ mRNA-seq library preparation kit FWD protocol for Illumina (Lexogen, Vienna, Austria). The RNA libraries were sequenced on an Illumina NextSeq 500 system with 75-nucleotide-long reads.