GSM8489714: 7_DU145sh6-2-1_1; Homo sapiens; RNA-Seq
Total RNA was extracted using NucleoSpin RNA Plus kit (Macherey-Nagel, Bethlehem, PA) and was then reverse transcribed with an iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA). Human FZD6 ORF was purchased from Origene (Rockville, MD). The shRNA-resistant mutant was generated using QuikChange II Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA).