GSM8795824: Ezhip_WT_Bl_K27me3_rep2; Mus musculus; OTHER
The fresh samples were incubated in antibody buffer (containing primary antibody and 2mM EDTA) overnight at 4 ℃. Samples were transferred to pA-MNase buffer after washed once with wash buffer via mouth pipetting. After incubation for 2-3 hours at 4 ℃, samples were bound with concanavalin-coated magnetic beads and equilibrated at room temperature for 10 min. MNase targeted digestion and DNA fragment extraction were performed. The library was constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Bio- labs, E7645S).