Long-read WGS library of Yersinia enterocolitica ATCC 9610 evolved under chloramphenicol selection
Long-read whole-genome sequencing libraries were prepared from high-molecular-weight genomic DNA extracted from antibiotic-evolved bacterial populations. Genomic DNA was isolated using the Monarch HMW DNA Extraction Kit for Microbes (NEB) following the Oxford Nanopore ultra-long read protocol. Libraries were prepared using the Oxford Nanopore Ultra-Long DNA Sequencing Kit without DNA fragmentation or barcoding and sequenced individually on MinION/Flongle R9.x flow cells to resolve genome structure and copy number variation.