title: GSM8947527: Con; Mus musculus; RNA-Seq
library_name: GSM8947527
library_construction_protocol: To induce AGA, the dorsal skin of 7-week-old male C3H/HeN mice was shaved, followed by subcutaneous injection of testosterone propionate (TP; 0.5mg/day). A CXCL12 neutralizing antibody (5 μg) was administered via subcutaneous injection twice weekly for two consecutive weeks. Skin tissues were collected from the dorsal region of mice (n = 15 per group). Samples were pooled, minced, and enzymatically digested in 0.7 mg/mL collagenase D (Sigma-Aldrich). The resulting suspension was filtered through cell strainers to remove aggregates. Red blood cells were lysed using ACK Lysing Buffer (Gibco), and debris was removed via density gradient centrifugation with Percoll (Sigma-Aldrich). The single-cell suspension was immediately used for single-cell RNA sequencing (scRNA-seq). Single-cell suspensions were processed using the Chromium GEM-X Single Cell 3' RNA v4 kit (10x Genomics), following the manufacturer's protocol. Briefly, cells were partitioned into Gel Beads-in-Emulsion (GEMs) containing barcoded oligonucleotides. Poly(dT) primers captured polyadenylated mRNA from individual cells, enabling the synthesis of barcoded cDNA. The resulting cDNA was amplified to construct 3' gene expression libraries and sequenced on an Illumina platform at Macrogen (Seoul, Republic of Korea).
identifiers.namespace: GEO
identifiers.id: GSM8947527_r1