title: DNA-Seq for mouse androgenetic blastomere
design: DNA extraction and amplification were performed using a commercial kit (Repli-G Single Cell; Qiagen, Hilden, Germany). Purified DNA was normalized at 20 ng/l and sent to an external laboratory (Genewiz; Azenta Life Sciences; South Plainfield, NJ) where a 150-bp paired-end exome sequencing on an Illumina HiSeq 2500 platform was performed, as previously described (33). Quality controls of each sample were performed by quantitative polymerase chain reaction, and poor-quality nucleotides were removed (error rate <0.01). Variant detection was performed using CLC Genomics Server 9.0, and the detected variants were annotated to identify unique gene mutations. All genomic coordinates were based on reference mouse genome assembly GRCm38 (mm10).
library_name: 30-871485633
identifiers.namespace: SUB14861835
identifiers.id: 30-871485633