title: GSM9033127: gastruloid differentiation day0 (0hr), Dox, rep1; Mus musculus; RNA-Seq
library_name: GSM9033127
library_construction_protocol: Gastruloid samples were lysed in 350 µl RLT buffer (Qiagen) with 3.5 µl β-mercaptoethanol and stored at –80°C until processing. For RNA extraction, 350 µl of 70% ethanol was added to the lysate, mixed thoroughly, and applied to Direct-zol RNA Microprep kit (Zymo) For each sample, 1 µg of RNA was used to prepare libraries with the Illumina stranded mRNA prep kit according to the manufacturer's protocol (10 PCR cycles for library amplification).
identifiers.namespace: GEO
identifiers.id: GSM9033127_r1