title: S_pombe_large_centromere_RNA-seq_rep1
design: Cells grown to OD600 ~0.5 in fresh YEL broth prior to extraction. RNA extracted with hot phenol + chloroform/isoamyl alcohol extraction method from Bähler and Wise, 2017. RNA cleanup was performed with the RNA Clean & Concentrator kit (Zymo Research). Ribosomal RNAs were depleted with FastSelect Yeast reagent (Qiagen) and RNA libraries were prepared with RNA-Seq FastSelect Depletion (stranded) (Watchmaker). 75bp paired-end sequencing was performed on libraries via the AVITI sequencer (Element Biosciences).
library_name: library6
identifiers.namespace: SUB16020557
identifiers.id: library6