title: GSM9696047: KO, CD8+T, UT; Mus musculus; ChIP-Seq
library_name: GSM9696047
library_construction_protocol: Genomic DNA was extracted under gentle conditions to preserve R-loops. Briefly, cells were lysed in lysis buffer containing SDS and proteinase K and incubated at 37°C overnight. Genomic DNA was then purified by phenol-chloroform extraction followed by ethanol precipitation. The DNA pellet was resuspended in TE buffer. Purified DNA was digested with a cocktail of restriction enzymes (EcoRI, BamHI, HindIII, BsrGI, and XbaI) to generate fragments of approximately 100-500 bp. DNA concentration was determined using a NanoDrop spectrophotometer, and samples were used for subsequent DRIP assays. DNA libraries for DNA-seq were prepared using standard Illumina protocols.
identifiers.namespace: GEO
identifiers.id: GSM9696047_r1