title: GSM8946998: donor with cataract surgery history, male, 71yr; Homo sapiens; RNA-Seq
library_name: GSM8946998
library_construction_protocol: To purify total RNA from lens tissues pre-treated with RNAlater, samples were homogenized using a FastPrep-24 5G homogenizer (MPBio, Santa Ana, CA), followed by total RNA extraction using the mirVana miRNA Isolation Kit (AM1560, ThermoFisher, Waltham, MA). RNA quality was assessed using a Bioanalyzer, and only high-quality samples with distinct rRNA peaks were selected for poly(A) RNA sequencing. Library preparation was performed using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760, New England BioLabs) with 11 PCR cycles.
identifiers.namespace: GEO
identifiers.id: GSM8946998_r1