title: GSM8471514: Colotomy_Mouse (mRNA and HTO); Mus musculus; RNA-Seq
library_name: GSM8471514
library_construction_protocol: Mouse bowel and MAT were both digested using digest media at 37C, with the bowel digested in two 30-minute fractions and MAT digested in 30 minutes. Samples were quenched using quenching media, filtered through a 70um filter, and centrifuged for 300g for 5 minutes. The supernatant was discarded and the pellet was resuspended in staining media (StemCell Tech) containing a 1:50 concentration of anti-human hashtag antibodies corresponding to the sample tube. The cells were washed and pelleted three times with staining media. The cells were resuspended at a concentration of 100 cells / uL in 0.25% BSA in PBS. Performed at the Stanford Genomics Facility (SGF) using the 10x Genomics platform. The relevant protocols were followed per manufacturer's guidelines.
identifiers.namespace: GEO
identifiers.id: GSM8471514_r1