title: GSM8465683: PROseq_KPC_BR2; Mus musculus; OTHER
library_name: GSM8465683
library_construction_protocol: We extracted total RNA, which includes biotin-labelled nascent RNA, using Trizol following the the manufacturer's protocol. the total RNA is subjected to three biotin-Streptavidin enrichment to select nascent RNA away from other RNA. We isolated nascent RNAs with magnetic beads coated with streptavidin, followed by 3' adapter ligation. After another round of biotin-streptavidin affinity purification, we removed the mRNA cap and ligated the 5' adapter. Following the third biotin-streptavidin affinity purification, we generated cDNA by reverse transcription and prepared PRO-seq libraries for sequencing using Illumina TruSeq small-RNA adaptors with twelve cycles of PCR
identifiers.namespace: GEO
identifiers.id: GSM8465683_r1