title: DNA of bacteria in beagle feces
design: DNA of LSbg3 strain was extracted and DNA concentration, purity and integrity were tested by Agilent 5400. Genome sequencing of LSbg3 was conducted by Novo on Illumina platform PE150 to remove low-quality bases and obtain Clean Data using SOAP denovo(version 2.04),SPAdes,ABySS assembly software for assembly, and CISA software for integration using gapclose(Version: 1.12) and other software optimized the preliminary assembly results and filled holes, so as to obtain the final assembly results. GeneMarkS(Version 4.17) were used to predict the coding genes of the sequenced genomes; Rfam(Version 1.2) and tRNAscan-SE(Version 1.3.1) were used to predict rRNA trnas and other Ncrnas, respectively. Functional annotations were obtained from the KEGG GO COG and SwissProt databases Prediction enzymes from the CAZy(Carbohydrate Active Enzymes,cazymes) database identified virulence factors and antibiotic resistance genes based on the core dataset of pathogen virulence factors (VFDB) and the Integrated Antibiotic Resistance Database (CARD)
library_name: LSbg3-1
identifiers.namespace: SUB14870895
identifiers.id: LSbg3-1