title: GSM8520383: C2C12, Daxx KO, rep3; Mus musculus; RNA-Seq
library_name: GSM8520383
library_construction_protocol: C2C12 cells were collected for RNA extraction using Machery Nagel NucleoSpin RNA purification kit Libraries were prepared with TruSeq Stranded Total RNA Sample preparation kit according to supplier recommendations. Briefly, the key stages of this protocol are successively, the removal of ribosomal RNA fraction from 1µg of total RNA using the Ribo-Zero Gold Kit; fragmentation using divalent cations under elevated temperature to obtain approximately 300bp pieces; double strand cDNA synthesis using reverse transcriptase and random primers, and finally Illumina adapters ligation and cDNA library amplification by PCR for sequencing
identifiers.namespace: GEO
identifiers.id: GSM8520383_r1