title: GSM9656474: WT MSC rep3; Mus musculus; RNA-Seq
library_name: GSM9656474
library_construction_protocol: The femurs and tibias were excised into bone chips and digested for 1 h with collagenase Ⅱ. Bone chips were then cultured in α-MEM supplemented with 10% FBS at 37 °C in a 5% CO2 incubator. Cells were sorted by GFP fluorescent signal to isolate Prx1+ MSCs PCR products were purified by AMPure XP system and library quality was assessed on the Agilent Bioanalyzer 2100 system. A TruSeq PE Cluster Kit v3-cBot-HS (Illumina) was used to cluster the index-coded samples. 150 bp paired-end reads were generated on an Illumina Novaseq platform.
identifiers.namespace: GEO
identifiers.id: GSM9656474_r1