title: RAT-E1 (CRX1929798; CRA029274)
library_name: missing
library_construction_protocol: Eukaryotic mRNA was enriched by Oligo ( dT ) magnetic beads, and the mRNA was randomly interrupted by Fragmentation Buffer.Then the first cDNA strand and the second strand were synthesized using mRNA as a template, and the cDNA was purified. The purified double-stranded cDNA was then subjected to terminal repair, A-tail was added, and the sequencing adapter was ligated. Then AMPure XP beads were used to select the fragment size. Finally, the cDNA library was obtained by PCR enrichment.
identifiers.namespace: NGDC
identifiers.id: CRX1929798
identifiers.namespace: Zunyi Medical University
identifiers.id: DRX812618