title: GSM9654317: Arsenite stressed, total RNA, replicate 1; Homo sapiens; RNA-Seq
library_name: GSM9654317
library_construction_protocol: Nuc/Cyto fractionation was done as previously described as in Bhatt et al., 2012; Ji et al., 2021; and letswaart et al., 2022 and detailed in accompying manuscript for this submission (Helton et al., 2026). RNA was sent to Biostate.ai for rRNA depletion using Probes for Excess RNA depletion and RNAseq.  For time course RNAseq of PolyA+ RNA, cells were scraped into DNA/RNA Shield (Zymo research) and sent for bulk RNAseq by Plasmidsaurus. For Biostate (nuc/cyto) library preparation: rRNA depleted RNA was reverse transcribed into cDNA and barcoded using Biostate's Barcode-Integrated Reverse Transcription technology.  For plasmidsaurus (time-course) library prepraration: PolyA+ RNA was converted into cDNA via reverse transcription and second-strand synthesis, followed by tagmentation, library indexing, and amplification.
identifiers.namespace: GEO
identifiers.id: GSM9654317_r1