title: Original stock before passaging
design: A tiling amplicon scheme was designed using PrimalScheme. The primers were pooled into 3 pools and used for RT-PCR using SuperScript VILO and Phusion U Multiplex Master Mix. The amplicons from each pool were purified and combined (within samples) at equivalent masses. Libraries were prepared using a Nextera DNA Flex kit and sequenced on an iSeq 100.
library_name: CHI-7913_P0
identifiers.namespace: SUB15470768
identifiers.id: CHI-7913_P0