title: GSM8637861: CN, RNAseq_CON_2; Mus musculus; RNA-Seq
library_name: GSM8637861
library_construction_protocol: Total RNA was extracted from two biological replicates of DIV13 primary cortical neurons (~5 million cells per replicate) using the Qiagen RNeasy Plus Universal Kit (Qiagen) according to the manufacturer's instructions. RNA quality was assessed using an Agilent Tapestation 4200, and only RNA with RIN score of 8 or higher was used for library preparation. Prior to library preparation, samples were treated with DNase to remove any residual DNA, and RNA concentration was measured by fluorometry (RNA high sensitivity Qubit assay, Thermo). RNA libraries were prepared for sequencing using a TruSeq stranded mRNA library prep kit with single-end adapters (Illumina) according to the manufacturer's instructions. Prior to sequencing, library quality was assessed using an Agilent Tapestation 4200, and sample concentration was measured by fluorometry (dsDNA high sensitivity Qubit assay, Thermo). Samples were pooled and sequenced on an Illumina NextSeq 500 instrument with V2.5 reagents to an average depth of 35-45 million reads per replicate.
identifiers.namespace: GEO
identifiers.id: GSM8637861_r1