title: PromethION sequencing
design: The library design aimed to sequence full-length MPXV genomes directly from cultured isolates using long-read Nanopore sequencing. Rapid barcoding was applied for multiplexing, and libraries were sequenced without fragmentation or enrichment.
library_name: lib_Cultured_MPXV_Skin_CA_TNP_20230209
library_construction_protocol: Libraries were constructed using the Oxford Nanopore Rapid Barcoding Kit V14 according to the manufacturerï¿½s protocol. No fragmentation was performed. Barcoded libraries were loaded directly onto a PromethION 2 Solo system with R10.4.1 flow cells.
identifiers.namespace: Helmholtz Institute for One Health
identifiers.id: ena-EXPERIMENT-TAB-07-08-2025-10:55:37:873-34377