title: GSM9113768: Molar, Cilk1; PCS1-MRCS1 KO homozygous, E14.5, rep3; Mus musculus; RNA-Seq
library_name: GSM9113768
library_construction_protocol: Maxillary molar tooth germs from Cilk1+/+, Cilk1−/− and Cilk1−/−; PCS1−MRCS1△/△ littermate mouse embryos were dissected at E14.5  (n = 6 per biological replicate; quadruplicates for the Cilk1+/+ and Cilk1−/− groups, and triplicates for Cilk1−/−; PCS1−MRCS1△/△ group). TRIZOL® RNA extraction technique  (Invitrogen) was used to extract total RNA. The RNA quality was evaluated following RNA ScreenTape®  (Agilent Technologies, Germany). Novogene  (China) prepared and sequenced the RNA library. Messenger RNA was isolated from total RNA using magnetic beads conjugated with poly-T oligos. After fragmentation, the first strand of cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. Once the library was constructed, it underwent amplification, purification, size selection, adapter ligation, end repair, and A-tailing. The quality of the library was assessed using a bioanalyzer for size distribution, a Qubit fluorometer, and real-time PCR for quantification. Finally, the quantified libraries were pooled and sequenced on the Illumina platform.
identifiers.namespace: GEO
identifiers.id: GSM9113768_r1