title: GSM9034006: Gaq_dBET6_n2; Homo sapiens; RNA-Seq
library_name: GSM9034006
library_construction_protocol: Cells were washed twice with cold PBS, and total RNA was extracted using the Qiashredder Homogenization kit and the RNeasy Mini kit (Qiagen) as per the manufacturer's instructions. RNA concentrations were measured with a Nanodrop 2000 spectrophotometer, and RNA quality was assessed on an Agilent 2100 Bioanalyzer Libraries were generated from 100 ng of total RNA as following: mRNA enrichment was performed using the NEBNext Poly(A) Magnetic Isolation Module (New England BioLabs). cDNA synthesis was achieved with NEBNext UltraExpress RNA Library Prep Kit (New England BioLabs) as per the manufacturer's recommendations. Adapters and PCR primers were purchased from New England BioLabs. Libraries were quantified using the KAPA Library Quanitification Kits - Complete kit (Universal) (Kapa Biosystems). Average fragment size was determined using a Fragment Analyzer 5300 (Agilent) instrument. The libraries were normalized and pooled and then denatured in 0.02N NaOH and neutralized using pre-load buffer. The pool was loaded at 140pM on a Illumina NovaSeq X Plus 25B lane following the manufacturer's recommendations. The run was performed for 2x100 cycles (paired-end mode) (150 base pair reads at a depth of ~25 million reads per sample for 24 samples). A phiX library was used as a control and mixed with libraries at 1% level. Program BCL Convert 4.2.4 was then used to demultiplex samples and generate FASTQ files.
identifiers.namespace: GEO
identifiers.id: GSM9034006_r1