title: GSM8471071: pilot_ATCC-BXS0116,DDX4-tdTomato/TFAP2C-mGreenLantern,Unsorted,hiPSC; Homo sapiens; Bisulfite-Seq
library_name: GSM8471071
library_construction_protocol: Genomic DNA was extracted using the Qiagen DNeasy Micro kit from hiPSCs and cells from the meiosis induction at various time points. For some of the samples, DDX4+ and DDX4- cells were sorted prior to extraction (see sample information). Libraries were prepared from 200 ng DNA using the NEB Enzymatic Methyl-Seq kit (NEB #E7120) following the manufacturer's instructions. Briefly, control DNA (methylated pUC19 and unmethylated λ phage) was spiked into each sample. Next, samples were sheared to ~300 bp using a Covaris S2 sonicator. Enzymatic methyl conversion was performed, and libraries were amplified using unique dual index primers. For the pilot experiment, 2 x 150 bp paired end sequencing was performed on an Illumina MiSeq. For the follow-up experiment, 2 x 150 bp paired end sequencing was performed on an Illumina NovaSeq. Methyl-seq (NEB enzymatic kit)
identifiers.namespace: GEO
identifiers.id: GSM8471071_r1