title: GSM9404976: KOR7; Mus musculus; OTHER
library_name: GSM9404976
library_construction_protocol: Lungs were excised from animals under sterile conditions and were collected into DNA extraction buffer containing ATL buffer and proteinase K (Qiagen) and shaken in a FastPrep Instrument (MP Biomedicals) at 5.0ms-1 for 30s. Following bead-beating, genomic DNA (gDNA) was extracted using the DNeasy Blood and Tissue Kit (Qiagen) in accordance with the manufacturer protocol. The PCR amplification of the target region was performed using 515F (GTGYCAGCMGCCGCGGTAA) and 806R (GGACTACNVGGGTWTCTAAT) 16S primers. Amplicons were then purified using NGS clean-up and size-select (Macherey-Nagel) in accordance with the manufacturer protocol.
identifiers.namespace: GEO
identifiers.id: GSM9404976_r1