title: GSM9033156: scRNA-Seq gastruloid differentiation day3 (72hrs), control, rep4; Mus musculus; RNA-Seq
library_name: GSM9033156
library_construction_protocol: On the day of collection, gastruloids were dissociated with accutase at 37°C for 5–10 minutes (ensuring minimal over-digestion). Cells were pelleted, washed twice with PBS, and resuspended in 0.04% BSA/PBS for cell counting. Viability was assessed using trypan blue, and cell numbers were determined on a hemocytometer with appropriate dilutions. Cells were diluted to the recommended density for the 10x Genomics Chromium Next GEM Single Cell 3' kit, targeting 10,000 cells per sample. Library prep were following the protocol described in 10x Genomics Chromium Next GEM Single Cell 3' kit
identifiers.namespace: GEO
identifiers.id: GSM9033156_r1