title: GSM8947389: VNO chip static 3; Homo sapiens; RNA-Seq
library_name: GSM8947389
library_construction_protocol: Using a needle, VNOs were meticulously extracted from the chip. For each sample, n = 2 independent microchannels were pooled together, and n = 3 samples were submitted for each condition (static, low flow, and high flow). RNA extraction was done using the Trizol protocol (Invitrogen). Sample quality control was performed using the Agilent 2100 Bioanalyzer or the Agilent 4200 Tapestation. Qualifying samples were then prepped following the standard protocol for the Illumina Stranded mRNA prep (Illumina). Sequencing was performed on the Illumina NextSeq2000 with Paired End 59bp x 59bp reads.
identifiers.namespace: GEO
identifiers.id: GSM8947389_r1