title: MinION sequencing
design: These FAST5 files were generated from a single library, run on a MinION for 36h, at which point the run stopped and was then restarted for a further 6 hours, so there are two runs of data, but all from the same library. These FAST5 files were base-called to generate FASTQ files using Guppy (version 6.1.7), and this fastQ Library was mapped (using minimap2) to a concatenated genome combining HVS-BAC sequence and Marmoset (Callithrix Jacchus) genome. The output BAM files were used alongside the FASTQ and FAST5 data as inputs to nanopolish v0.14 to determine poly(A) tail lengths.
library_name: OMK_HVS24_48hpi_dRNA
library_construction_protocol: RNA was extracted from OMK cells 48 hours after infection at MOI 2500 genomes per cell, using TRIZOL with two additional 70% ethanol washes, and stored as an ethanol pellet at -80 C until library preparation. PolyA-based library preparation was performed with the RNA002 kit (Oxford Nanopore Technologies) including reverse transcription with Induro reverse transcriptase(NEB), followed immediately by direct RNA sequencing using a MIN106D version R9.4.1 flow cell.
identifiers.namespace: Section of Virology, Dept of Infectious disease,
identifiers.id: ena-EXPERIMENT-TAB-12-12-2025-13:49:43:282-337015