title: amplicon sequencing of 16S : soil
design: Total genomic DNA extraction was performed using the Qiagen DNeasy PowerSoil Pro extraction kit. PCR amplification of the V3-V4 region of the 16S rRNA gene was sequenced using 341F and 806R primers (CCTAYGGGRBGCASAG; GGACTACNNGGGTATCTAAT (Herlemann et al., 2011; Wasimuddin et al., 2020)). Illumina Sequencing MiSeq technology (paired-end reads). Data was processed across two sequencing runs.
library_name: Restoration_soil_site2_bac_4
identifiers.namespace: SUB14868246
identifiers.id: Restoration_soil_site2_bac_4