title: Brassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene Expression
design: Brassinosteroids Enhance Barley De-Acclimation Tolerance via Modulation of Chloroplast- Associated Gene Expression
library_name: Sample 2_p
library_construction_protocol: Leaves from each mutant and Bowman cultivar were sampled after cold acclimation (CA) and after 24 h after de-acclimation (DA) in three biological replicates (leaves from three different plants). The leaves were immediately frozen in liquid nitrogen after sampling and stored at −80°C until use. Each sample was collected from the middle part of the youngest fully developed leaf and weighted 0.03–0.05 g. Plants were sown in pots (30 cm wide and 15 cm long) and grown for 3 weeks in a greenhouse at 18 °C (day/night) in daylight (the experiment was conducted in November in Krakow, Poland, 50°03' N, 19°55' E). Next, the plants were placed in a growth chamber for cold acclimation at a temperature of 2 °C/2 °C (day/night), with a photoperiod of 8 h/16 h (day/night) and 250 umol m-2 s-1 PPFD for 3 weeks. Half of plants were subjected to cold acclimation only (without de-acclimation) and sampled after beeing kept for 3 weeks in hardening conditions. They served as control for the experiment. Total RNA was isolated from 18 leaf samples using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). mRNA was isolated using oligo(dT)-attached magnetic beads, and cDNA libraries were constructed, converted into single-stranded circular DNA (ssCir DNA)
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-15311:Sample 2_p