title: GSM9441723: OCI-AML3 Vehicle Sample 3 Sequencing Batch 2; Homo sapiens; RNA-Seq
library_name: GSM9441723
library_construction_protocol: total RNA was isolated using the Qiagen RNeasy Mini Kit, following the manufacturer's protocol Libraries were generated from 200 ng of total RNA using the Roche Kapa mRNA HyperPrep strand-specific kit according to the manufacturer's guidelines, automated on a Beckman Coulter Biomek i7 Double-stranded DNA libraries were quantified using a Qubit fluorometer and Agilent TapeStation 4200. Dual-indexed libraries were pooled in equimolar amounts and initially shallow-sequenced on an Illumina MiSeq to assess library concentration and pooling accuracy. The final library pool was sequenced on an Illumina NovaSeq6000 with a target of 40 million 100 bp paired-end reads per sample
identifiers.namespace: GEO
identifiers.id: GSM9441723_r1