title: metabarcoding of fungi of Picea glauca roots
design: For DNA extraction, 40 fragments of visible ectomycorrhiza (ECM) root tips were picked from each of six roots, resulting in 240 fragments of ECM per sample. The DNA extraction procedure employed the NucleoSpin Soil extraction kit (Macherey Nagel, Dren, Germany). Amplification of ITS2 region as a barcode, library preparation and Illumina MiSeq sequencing were conducted according to Kuprina et al. (2025).
library_name: DF45570-r1
identifiers.namespace: SUB15273490
identifiers.id: DF45570-r1