title: Genomic reads generated through a double-digestion restriction-site associated DNA sequencing protocol (ddRADseq) for a subset of individuals belonging to the species Lepromoris gibba and Lepromoris exophthalmus.
design: Genomic DNA was extracted from each individual using the Biosprint DNA Blood Kit (Qiagen) on a Thermo KingFisher Flex automated extraction instrument. To generate genome-wide nuclear data, individual genomic DNA was processed using the double-digestion restriction-site associated DNA sequencing protocol (ddRADseq, Peterson et al., 2012), as described in Salces-Castellano et al. (2020), and Noguerales and Emerson (2025). In brief, individual DNA extracts were digested with the restriction enzymes MseI and EcoRI (New England Biolabs). Resulting genomic libraries were then pooled at equimolar ratios, followed by size selection for fragments between 200 and 250 base pairs (bp), and paired-end sequenced (150 bp) on an Illumina NovaSeq6000 (Novogene, Cambridge, UK).
library_name: EH_EOL_1550
identifiers.namespace: SUB16111450
identifiers.id: EH_EOL_1550