title: RNA-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions
design: RNA-seq of wild-type mouse PSM tissues that were microdissected into tailbud, posterior PSM, anterior PSM, and somite regions
library_name: RNA_20191009_NA3_s
library_construction_protocol: Mice were crossed, and females were dissected 10 days after a detection of vaginal plug, and the PSM tissues isolated. PSM explants of E10.5 wild-type embryos were microdissected into tail bud, posterior PSM, anterior PSM, and somite regions by micro scalpel in cold PBS. Each tissue region was transferred into a micro well (ibidi, #80486) and mechanically dissociated to a cell suspension in 4.2 �l cold PBS. Finally, 0.7 �l and 3.3 �l cell suspensions were used for RNA-sequencing (RNA-seq) and ATAC-sequencing (ATAC-seq), respectively RNA-seq libraries were prepared following the Smart-seq2 described in Picelli et al., Nat Protoc, 2014
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-13693:RNA_20191009_NA3_s