title: GSM9266110: multiome ATAC-seq, human iPSC-derived microglia, LRRK2 wildtype, control, rep2; Homo sapiens; ATAC-seq
library_name: GSM9266110
library_construction_protocol: Differentiated iPSc-derived microglia cells were washed with 1x PBS, Accutase solution was added to each well and cells were incubated at RT for 10 min. Next, cells were collected in 1xPBS by centrifugation, 300g, 5min and washed once with 1xPBS + 0.04% BSA. 100 μl of chilled Lysis Buffer (10mM Tris-HCl (pH 7.4), 10mM NaCl, 3mM MgCl, 1% BSA, 0.1% Tween-20, 0.1% NP40, 0.01% Digitonin) were added to 500,000 cells, cells were resuspended 10 times, and incubated on ice for 5 min. Next, 1 ml of chilled Wash Buffer (10mM Tris-HCl (pH 7.4), 10mM NaCl, 3mM MgCl, 1% BSA, 0.1% Tween-20) was added to the lysed cells and mixture was pipetted 5 times. Nuclei were precipitated at 500g for 5 min at 4°C. Nuclei were washed in the Wash  Buffer for a total of 3 washes. 20,000 nuclei/sample were processed according to  Chromium next GEM Single cell Multiome ATAC+Gene Expression user guide (CG000338 Rev E). Chromium Single Cell Multiome  Gene Expression and ATAC libraries were sequenced using standard Illumina® paired-end sequencing with P5 and P7 primers.
identifiers.namespace: GEO
identifiers.id: GSM9266110_r1