title: GSM8472282: NC8; Mus musculus; RNA-Seq
library_name: GSM8472282
library_construction_protocol: the murine cPTAAs or sham-operated proximal thoracic aortas were harvested and stored in liquid nitrogen. Next, snap-frozen tissues were ground into powder using a mortar. A proper volume TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) was then added to the mortar, and total RNA was extracted. the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) was used to assess the RNA integrity. About 500ng RNA per sample underwent mRNA capture (oligo dT magnetic bead capture method), mRNA fragmentation, the first strand of cDNA synthesis using random hexamer primer and M-MuLV Reverse Transcriptase(RNase H), the second strand of cDNA synthesis using DNA Polymerase I and RNase H, A tail and sequencing adapter addition, cDNA fragments(with sequencing adapter) screening using magnetic beads, amplification and cleaning of the library, and quality control steps.
identifiers.namespace: GEO
identifiers.id: GSM8472282_r1