title: GSM9116267: NCI-H446 UHRF1 KO 5wk tumor 5; Homo sapiens; RNA-Seq
library_name: GSM9116267
library_construction_protocol: Total RNA from homogenized tumors was isolated using RNeasy Mini Kit (Qiagen). After quality check with Agilent BioAnalyzer and Qubit, 1 µg RNA per sample was used for poly(A) mRNA isolation. cDNA libraries were prepared using NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina, following manufacturer instructions. Barcode-indexed libraries were purified with AMPure XP beads (Beckman Coulter) and checked for library quality using Agilent BioAnalyzer DNA Chip and quantified using KAPA qPCR.
identifiers.namespace: GEO
identifiers.id: GSM9116267_r1