title: GSM9266380: PCLS-Control-1; Homo sapiens; RNA-Seq
library_name: GSM9266380
library_construction_protocol: Human PCLS specimens were harvested 5 days after treatment with the FC, with or without 4-IPP. For each experimental group, three PCLS slices were pooled to generate sufficient nuclei for library preparation and sequencing.Nuclei were isolated from human snap frozen lung tissue using the Chromium10x genomics nuclei isolation kit, per manual. An aliquot of nuclei was stained with 0.4% trypan blue stain and DAPI and subsequently counted with the Thermo Fisher countess II FL. Samples with > 90% viability were used for downstream analysis. Approximately 20,000 nuclei per sample were loaded onto chip M and processed with the Chromium X controller. Barcoding and DNA (cDNA) library preparation was performed according to the manufacturer's protocol (Chromium Next GEM Single Cell 3' HT Kit v3.1). Quality of the cDNA was assessed at 2 time points with the Agilent Bioanalyzer High sensitivity DNA chip. The cDNA libraries were sequenced on the Novaseq 6000, using a Hiseq paired end 100bp sequencing configuration.
identifiers.namespace: GEO
identifiers.id: GSM9266380_r1