title: DNA Metagenomics of Human Brain Hippocampi : Alzheimer's Disease Female
design: Metagenomic sequencing performed by Novogene Co. using human hippocampi DNA extracts. Shear genomic DNA into short fragments. Perform end repair, A-tailed, and ligated with Illumina adaptor. PCR amplified, size selected, and purified. Check the library with Qubit. Real-time PCR for quantification and bioanalyzer for size distribution detection. Novogene pooled and sequenced the quantified libraries on Illumina platforms according to effective library concentration and data amount required. Other: Library strategy was WBI Shotgun Metagenomics Sequencing.
library_name: Subject_3_AD
identifiers.namespace: SUB14872298
identifiers.id: Subject_3_AD