title: GSM8607851: L4_1; Mus musculus; RNA-Seq
library_name: GSM8607851
library_construction_protocol: Total RNA from 5 sex and litter-matched pairs (2 pairs of male mice and 3 pairs of female mice) of P18 cortical tissues of Usp7 WT; Trp53+/- and Usp7 cKO; Trp53+/- mice was extracted with RNeasy Plus Mini Kit (QIAGEN) for RNA-seq analysis. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers to ensure complete coverage of gene body. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags.
identifiers.namespace: GEO
identifiers.id: GSM8607851_r1