title: Nanopore sequencing reads of Gracilimonas pinellae BCB1
design: Colony was grown in BD Difco Marine Broth 2216 at room temperature in the dark. Upon visible growth, the culture was centrifuged at 1,700 RCF for 5 minutes. DNA was extracted from the pellet using Qiagens AllPrep DNA/RNA Mini kit and purified using Zymo Researchs Genomic DNA Clean & Concentrator-10. The purified DNA was quantified using the Qubit HS assay and the Nanodrop ND-1000 spectrophotometer, then visualized on a 1% (wt/vol) agarose gel before submission to Eurofins Genomics for Nanopore sequencing using the v14 library preparation chemistry, R10.4.1 flow cell and dna_r10.4.1_e8.2_400bps_hac@v4.2.0 basecalling model.
library_name: Gracilimonas
identifiers.namespace: SUB15342139
identifiers.id: Gracilimonas