title: Metagenome of mus muscululs: cecum
design: DNA was extracted from the cecal contents using the QIAamp Fast DNA Stool Mini Kit (Qiagen, Venlo, Netherlands) following the manufacturer's instructions. The V3-V4 region of 16S rRNA was amplified through PCR using the primers 341F and 805R, and KOD FX (Toyobo). The amplified PCR product was purified with Agencourt AMPure XP Beads (Beckman Coulter, CA, USA) and sent to Macrogen Japan Corp. (Tokyo, Japan) for next-generation sequencing.
library_name: aw-2
identifiers.namespace: SUB16080878
identifiers.id: aw-2