title: GSM8519240: AsiSI_U2OS_WEE1i_4OHT_BrdU_seq_Figure4a_EDF9f_replicate 1; Homo sapiens; OTHER
library_name: GSM8519240
library_construction_protocol: DNA from cell cultures was isolated size fractionated and subject to exunuclease digestion as described in Aladjem et al. Science 281 1005 1998. and Wang et al. Molecular and Cellular Biology 24:3373-86 2004. For ChipSeq DNA molecules were enriched using antibodies against proteins of interest. For BrdU seq extraction was performed using a protocol modified from Marchal et al. 2018 Samples were eluted and then purified by Monarch PCR & DNA Cleanup Kit (NEBT1030S). 100 ng of samples were used to generate the library. The kits used for the library were NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB E7805S) and NEBNext Multiplex Oligos for Il-lumina (Index Primers Set 1 and Set 2) (NEB E7335S and E7500S).
identifiers.namespace: GEO
identifiers.id: GSM8519240_r1