title: scRNA-Seq of mus musculus: adult male skin
design: According to the manufactures introduction, Single-cell RNA-seq libraries were constructed using Single Cell 3 Library and Gel Bead Kit V3.1. Briefly, combine the prepared single cell suspension with the mixture of gel beads containing barcode information and enzymes, and then wrap it with oil drops located in the "single cross" connection of microfluidics to form GEMs (Gel Bead In EMulsions). In the effective GEMs, 10x Barcode will be linked to the cDNA product, and then the GEMs will be broken and oil droplets will be crushed. PCR amplification will be performed using cDNA as a template. After cDNA amplification is completed, quality inspection will be conducted on the amplified product. Firstly, the cDNA is broken into fragments of about 200-300 bp using chemical methods. The cDNA is fragmented, end repaired, and A is added for cDNA fragment screening. The P7 Adapter adapter is connected and introduced into the sample index through PCR amplification. Finally, fragment screening is performed to obtain the cDNA library.
library_name: CTRL_2
identifiers.namespace: SUB14666715
identifiers.id: CTRL_2