title: GSM9033803: Lung_wt_e15.5_ATAC_seq_rep3; Mus musculus; ATAC-seq
library_name: GSM9033803
library_construction_protocol: Lungs from E15.5 mouse embryos were isolated, snap-frozen in liquid nitrogen and kept at -80oC until use. Nucleus extraction and tagmentation were performed as described (Grandi et al., 2022). Tagmentation with Tn5 transposase (Diagenode, C01070012) was done on 60,000 nuclei. The NEBNext Ultra II DNA library prep kit from Illumina (New England Biolabs) was used to prepare DNA libraries, according to manufacturer's instructions, from 2 ng of fragmented ChIP DNA, followed by the addition of NEBNext adaptors and purification with AxyPrep Mag PCR Clean-up kit (Axygen).
identifiers.namespace: GEO
identifiers.id: GSM9033803_r1