title: GSM9656520: UPM30_Rep1_D0; Homo sapiens; ATAC-seq
library_name: GSM9656520
library_construction_protocol: Cells were washed twice with ice-cold 1X PBS and collected by scraping (pooling 3 wells/treatment) and pelleting prior to counting. Approximately 50K cells were pelleted and processed in duplicate as described in the Omni-ATAC-seq protocol published by Corces et al (Nature Methods, 2017). Uniquely indexed sequencing adapters from the Nextera DNA CD Indexes Kit were added to both ends of transposed fragment libraries by PCR. Following final purification, libraries were pooled for sequencing on an Illumina NovaSeq 6000 instrument using 150 bp paired-end reads at the University of Colorado Anschutz Medical Campus Cancer Center Genomics Shared Resource Core Facility.
identifiers.namespace: GEO
identifiers.id: GSM9656520_r1