title: amplicon sequencing of ITS : soil
design: Total genomic DNA extraction was performed using the Qiagen DNeasy PowerSoil Pro extraction kit. PCR amplification of ITS region of the fungal rRNA cluster was performed with primersprimer ITS1F specific for higher fungi (CTTGGTCATTTAGAGGAAGTAA, (Gardes and Bruns, 1993)) and the general primer ITS2 (GCTGCGTTCTTCATCGATGC, (White et al., 1990)). Illumina Sequencing MiSeq technology (paired-end reads). Data was processed across two sequencing runs.
library_name: Paddock_soil_site2_fun_1
identifiers.namespace: SUB14868246
identifiers.id: Paddock_soil_site2_fun_1