title: Fungal (ITS) microbiome of soil: without soil or any added substances
design: DNA was extracted without fresh frozen soil using the DNeasy PowerSoil Pro Kit (QIAGEN N.V., Germany) following the manufacturers protocol. Fungal community composition was assessed by amplifying the ITS region using primer pair CS1_ITS1F and CS2_ITS2R (Core Research Resource Center, University of Illinois). Library preparation for sequencing involved two rounds of PCR. The first PCR reaction mixture (20 L total volume) consisted of 1 L of normalized template DNA, 0.2 L of DreamTaq DNA Polymerase (5 U/L), 2 L of 10 DreamTaq Buffer, 2 L of 1 mM dNTPs, 0.6 L each of forward and reverse primers (10 M), and 13.6 L of autoclaved Milli-Q water. Amplicons were sequenced on an Illumina MiSeq platform using a V3 600-cycle kit (2  300 bp paired-end reads).
library_name: Leonardo096_Fang-Blank_L001
identifiers.namespace: SUB15371376
identifiers.id: Leonardo096_Fang-Blank_L001