title: ChIP-Seq of Cellula squamosa oris humana WSU-HN30: Immunoprecipitation Overexpression circE7
design: Chromatin was first crosslinked with formaldehyde to preserve DNA-protein interactions and then sonicated to shear the chromatin into fragments of 200-500 bp. Immunoprecipitation was performed using antibodies specific to the target protein, enriching DNA fragments bound by the protein of interest. The crosslinks were then reversed, and the DNA was purified using phenol-chloroform extraction or purification columns. The purified DNA was end-repaired, A-tailed, and adaptor-ligated using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA) according to the manufacturer's protocol. Size selection was performed to enrich for fragments of 200-400 bp using AMPure XP beads (Beckman Coulter, Brea, CA, USA). The adaptor-ligated DNA was then amplified by PCR to generate sufficient material for sequencing. The final libraries were purified using the AMPure XP system and assessed for size distribution using the 2100 Bioanalyzer (Agilent, Santa Clara, CA). Quantification was performed using real-time PCR. Sequencing was carried out on the Illumina NovaSeq X Plus platform using paired-end technology (PE 150).
library_name: OEcircE7_IP
identifiers.namespace: SUB14671809
identifiers.id: OEcircE7_IP