title: GSM8472329: leaf,DS+Y-S,rep2; Sesbania grandiflora; RNA-Seq
library_name: GSM8472329
library_construction_protocol: The purity, concentration and integrity of RNA samples are tested using advanced molecular biology equipment to ensure the use of qualified samples for transcriptome sequencing. Eukaryotic mRNA enrichment with magnetic beads with Oligo(dT). Add Fragmentation Buffer to randomly interrupt the mRNA. The first cDNA strand and the second strand were synthesized using mRNA as a template, and cDNA purification was carried out. The purified double-stranded cDNA is then repaired at the end, A-tailed and ligated with sequencing adapters, and then slipped with AMPure XP beads segment size selection. Finally, the cDNA library was obtained by PCR enrichment. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads.
identifiers.namespace: GEO
identifiers.id: GSM8472329_r1