title: Pore-C of Saccharum hybrid POJ2878: young leaf
design: Pore-C libraries were generated to study multi-way chromatin contacts in Saccharum hybrid POJ2878. In-situ chromatin conformation capture was performed by cross-linking cells with formaldehyde, followed by digestion with HindIII. Interacting DNA fragments were ligated under proximity conditions to form long concatemers. After reversing cross-links, the high-molecular-weight DNA containing multi-fragment ligation products was purified. A sequencing library was prepared using the Ligation Sequencing Kit SQK-LSK114 from Oxford Nanopore Technologies, without further fragmentation. The final library was sequenced on an Oxford Nanopore PromethION platform R10.4 chip to capture long reads spanning multiple interacting loci.
library_name: POJ2878_PoreC_4_1
identifiers.namespace: SUB15530546
identifiers.id: POJ2878_PoreC_4_1