title: adult female lung
design: A library of CRISPR mutants was constructed in the M. tuberculosis H37Rv strain using CRISPR-NHEJ. C57BL-6N mice were intra-infected with 4X 107 colony forming units of the mutant library. At indicated times post-infection, viable bacteria (3X 104 clones) were recovered from lung homogenates by plating on 7H10 agar medium (in vivo pool). 4 mice were sacrificed at the 2-week time point and 4 mice were used at the 4-week time point. In vitro pools were generated by re-culturing the library. Genomic DNA was isolated from each pool and CRISPR probes were generated for each pool to hybridize to the microarray.
library_name: 0d
identifiers.namespace: SUB15246921
identifiers.id: 0d