title: GSM8636721: post-dissociation2; Mus musculus; RNA-Seq
library_name: GSM8636721
library_construction_protocol: For “pre-dissociation,” we collected 3 kidneys per sample, and for “post-dissociation,” we extracted over 1 × 10^6 cells with viability of over 80% from 8 kidneys per sample using the aforementioned treatment protocol. RNA extraction and DNA removal were then performed following the protocol with the RNeasy Plus Micro Kit (74034, QIAGEN). We confirmed that each sample yielded at least 66 ng of extracted RNA, which was stored at -80°C Reverse transcription was carried out using the SMART-Seq v4 Ultra Low Input RNA kit (634892, Takara Bio), followed by library preparation using the Nextera XT DNA Library Prep Kit (FC131-1024 and FC-131-1096, Illumina).
identifiers.namespace: GEO
identifiers.id: GSM8636721_r1