title: GSM8946894: ATAC-seq of glioma sample MG11 (Anaplastic oligodendroglioma, Grade III, Primary, IDH1 Mut); Homo sapiens; ATAC-seq
library_name: GSM8946894
library_construction_protocol: Nuclei were isolated from patient tumor tissue as described by Habib et al., with slight modifications. Frozen tissue samples (<0.5 cm) were incubated on ice in 1 mL of Nuclei EZ Lysis Buffer (Sigma, #Nuc-101). The tissue was homogenized using a glass tissue grinder. Additional EZ Lysis Buffer was added, incubated on ice for 5 min, and filtered through a 70 μm cell strainer. Nuclei were collected by centrifugation and resuspended in ATAC-resuspension buffer. ATAC-seq library preparation was performed as previously described by Buenrostro et al. Nuclei were filtered through 40 μm and 5 μm cell strainers and resuspended in ATAC-resuspension buffer (10 mM Tris–HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, and 0.1% Tween-20).
identifiers.namespace: GEO
identifiers.id: GSM8946894_r1