title: RNA-seq of the plasmid transconjugant Se477(pADAP)
design: RNA was stabilised using RNA protect bacteria (Qiagen) following manufacturers instructions, before being isolated using the RNA mini kit (Qiagen). On-column digest with RNAse free DNAse followed by a second off column digest. Following mini kit protocol, eluted RNA was precipitated and resuspended in RNAse free water before being shipped to Macrogen (South Korea). RNAseq was quality controlled using the TruSeq standard total RNA kit and Novaseq 6000 platform generated 150bp paired-end reads.
library_name: RNA_Se477(pADAP)-1
identifiers.namespace: SUB15370482
identifiers.id: RNA_Se477(pA1M02)-1