title: PARIS_dead_rep3_raw_reads
design: total RNA from T5 phage-infected cells was extracted using Direct-zol RNA Kit (Zymo Research), sheared with ultrasound, and polyadenylated using E. coli Poly(A) Polymerase (New England Biolabs). Amplicon were prepared from polyadenylated RNA and barcoded using  cDNA-PCR Barcoding Kit V14 from Oxford Nanopore (SQK-PCB114.24). Sequencing data was basecalled using dorado with super-accuracy model and --no-trim option.
library_name: PARIS_dead_rep3_raw_reads
identifiers.namespace: SUB15369968
identifiers.id: PARIS_dead_rep3_raw_reads