title: GSM9264358: OTM 1d (mouse alveolar bone, right first molar, pooled 10); Mus musculus; RNA-Seq
library_name: GSM9264358
library_construction_protocol: Alveolar bone tissues within 1 mm of the maxillary first molar (gingiva removed) were dissected, rinsed in ice-cold PBS with 1% FBS, minced into 1–2 mm fragments, and digested with 2 mL GEXSCOPE® Bone Tissue Dissociation Solution at 37 °C for 60 min. Cell suspensions were filtered, centrifuged, and red blood cells were removed with GEXSCOPE® RBC lysis buffer Single-cell suspensions (700–1200 cells/μL) were loaded onto the 10x Genomics Chromium Next GEM system, and libraries were prepared using the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (PN-1000268) according to the manufacturer's protocol. The resulting libraries were sequenced on an Illumina NovaSeq 6000 platform (paired-end 150 bp).
identifiers.namespace: GEO
identifiers.id: GSM9264358_r1