title: GSM8519128: RPE dsDNA biol rep 2; Homo sapiens; ssRNA-seq
library_name: GSM8519128
library_construction_protocol: Cells were lifted by 0.25% Trypsin-EDTA, quenched with conditioned culture media, and washed with 1x PBS. Total RNA was then isolated using the RNEasy kit (Qiagen) per manufacturers protocol. Poly-A(+) RNA enrichment, and strand-specific library preparation were carried out using a Tecan/Nugen Universal Plus mRNA Kit. Paired-end 150 bp sequencing was carried out on an Illumina NovaSeq 6000 instrument by the University of Colorado Cancer Center Genomics Shared Resource.
identifiers.namespace: GEO
identifiers.id: GSM8519128_r1