title: RNA-Seq of SARS-CoV-2 Nucleocapsid protein expression in human embryonic kidney cell line HEK293
design: RNA-Seq of SARS-CoV-2 Nucleocapsid protein expression in human embryonic kidney cell line HEK293
library_name: 91_p
library_construction_protocol: Cells were harvested for RNA extraction 48 hours post-transfection using RNATri (Bio&Sell). Human embryonic kidney 293 (HEK293) cells were seeded in 6-well plates and grown to 75% confluence in DMEM high glucose with 10% FBS.  1 µg plasmid of pCDNA3.1-HA-SARS-CoV2 N were transfected using lipofectamine 2000 (Invitrogen). Total RNA was extracted from RNATri (Bio&Sell) samples, followed by DNase I digestion to remove contaminating DNA. Final RNA concentration was adjusted to 250 ng/µL using RNase-free water. 3 independent samples were prepared for each condition. Libraries were prepared using the mRNA enrichment method at BGI Genomics.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14342:91_p