title: MinION sequencing
design: FAST5 files were base-called to generate FASTQ files using Guppy (version 6.1.7), and this fastQ Library was mapped (using minimap2) to a concatenated genome combining HVS-BAC sequence and Marmoset (Callithrix Jacchus) genome. The output BAM files were used alongside the FASTQ and FAST5 data as inputs to nanopolish v0.14 to determine poly(A) tail lengths.
library_name: 293-HB9-A4_48hpr_dRNA
library_construction_protocol: RNA was extracted from 293 cells carrying latent B95-8 EBV, 48 hours after reverse transfection of BZLF1, BRLF1 and BALF4 expression constructs to induce reactivation, using TRIZOL with two additional 70% ethanol washes, and stored as an ethanol pellet at -80 C until library preparation. PolyA-based library preparation was performed with the RNA002 kit (Oxford Nanopore Technologies) including reverse transcription with Induro reverse transcriptase(NEB), followed immediately by direct RNA sequencing using a previously used and washed MIN106D version R9.4.1 flow cell.
identifiers.namespace: Section of Virology, Dept of Infectious disease,
identifiers.id: ena-EXPERIMENT-TAB-11-12-2025-16:03:00:042-349959