title: Solanum lycopersicum leaf FsK-untreated Drought-stress replicate 3
design: Total RNA was isolated from leaf tissue using LiCl precipitation and treated with DNase I (Thermo) to remove genomic DNA contamination (Brusslan & Tobin, 1992). RNA quality and quantity were assessed, and 10 ng of total RNA per sample was used for gene expression quantification using the Luna Universal One-Step RT-qPCR Kit (NEB, USA). For transcriptome analysis, RNA-seq libraries were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) following the manufacturers instructions. Libraries were sequenced on an Illumina NovaSeq 6000 to generate single-end reads of 100 bp. The experiment included 3 biological replicates per condition.
library_name: lib_nofsk_drought_rp3
identifiers.namespace: SUB15838504
identifiers.id: lib_nofsk_drought_rp3