title: GSM8471423: RAW_H37Ra_ATACseq_rp2; Mus musculus; ATAC-seq
library_name: GSM8471423
library_construction_protocol: RAW264.7 cells were plated in triplicate in 12-well plates at a seeding density of 2×10^5 cells per well one day before the experiment. After 24 hours of Mtb infection, cells were harvested, washed with PBS, and resuspended in lysis buffer. Nuclei were pelleted by centrifugation and transferred to transposition reaction buffer, followed by incubation at 37°C for 30 minutes. Genomic DNA was extracted using the Qiagen MinElute PCR Purification Kit. ATAC-seq library preparation was performed according to the protocol described by Buenrostro et al. (2015). The purified ATAC-seq DNA library mix was sequenced on an Illumina NovaSeq 6000 platform. The ATAC-seq Library preparation was performed through the manufacturer's introduction.
identifiers.namespace: GEO
identifiers.id: GSM8471423_r1