title: GSM9265550: B220; Mus musculus; RNA-Seq
library_name: GSM9265550
library_construction_protocol: After 6 weeks of Western diet, aortas were dissected under a microscope, and adventitia was removed to preserve the media and intima. Tissue was homogenized, and total RNA was isolated using the RNeasy Fibrous Tissue Mini Kit (Qiagen). RNA integrity and concentration were assessed with Qubit RNA HS Assay Kit and Agilent Fragment Analyzer. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit with TruSeq RNA UD Indexes according to the manufacturer's protocol. Libraries were quality controlled with Qubit dsDNA HS Assay Kit and Agilent Fragment Analyzer, pooled, and sequenced on an Illumina NovaSeq 6000 platform (paired-end, 50 bp).
identifiers.namespace: GEO
identifiers.id: GSM9265550_r1