title: GSM8472154: CG11247 (Saft)-wt RNA-seq ovary, rep2; Drosophila melanogaster; RNA-Seq
library_name: GSM8472154
library_construction_protocol: RNA was prepared as described in ElMaghraby et al. 2019. In brief, per replicate five ovary pairs or forty brains from 3-4-day old female flies were homogenized in TRIzol (Invitrogen) with a plastic pestle. Total RNA was TRIzol-extracted and purified using the RNA Clean & Concentrator kit (Zymo research) with on-column DNase I digest according to the manufacturer's instructions. Ribosomal RNA was depleted starting with 1ug (ovary) or 0.5ug (brain) total RNA using custom oligonucleotides complementary to D. melanogaster rRNAs (listed in ElMaghraby et al. 2019) and Hybridase Thermostable RNase H (LGC Biosearch Technologies). Libraries of rRNA depleted samples were generated using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8472154_r1