title: GSM9579624: EBV_ATAC; Mus musculus; ATAC-seq
library_name: GSM9579624
library_construction_protocol: Whole bone marrow (BM) cells were incubated for 20 min with a purified rat IgG2a anti-mouse CD16/CD32 antibody (BioLegend) to block Fc receptor-mediated nonspecific binding. Cells were then stained for 30 min at 4°C with APC-conjugated antibodies against lineage markers (CD3, B220, Gr-1, Ter119, NK1.1, SiglecF, and CD11b). The cell suspension was subsequently incubated with anti-APC magnetic beads (BioLegend) for 15 min. Cells were passed through LS columns, and the negative (flow-through) fraction was collected and used for subsequent sorting of LSK cells. For bone marrow monocytes, T cells, CMP/GMP populations, and blood monocytes, cells were incubated with an Fc receptor–blocking antibody followed by staining with fluorochrome-conjugated antibodies against surface markers. Cell sorting was performed using a BD FACSAria™ III cell sorter. Gating strategies are shown in the manuscript. Libraries were generated using the Chromium Single Cell Multiome ATAC + Gene Expression Kit (10x Genomics) following the manufacturer's instructions. Libraries were sequenced on an Illumina NovaSeq 6000 platform.
identifiers.namespace: GEO
identifiers.id: GSM9579624_r1