title: GSM8519450: control1; Homo sapiens; RNA-Seq
library_name: GSM8519450
library_construction_protocol: Total RNA was extracted from cells using TRIzol reagent (Invitrogen, OR). Ribosomal RNA was removed by poly-A selection using Oligo-dT beads (mRNA Direct kit, Life Technologies). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags.
identifiers.namespace: GEO
identifiers.id: GSM8519450_r1