title: GSM8465961: [eYFP+ B cells and PC][C57BL/6F] [H9N063I6]; Mus musculus; RNA-Seq
library_name: GSM8465961
library_construction_protocol: The protocol was performed as previously described by Attaf et al. (DOI : 10.3389/fimmu.2020.00216). Individual cells were sorted into a 96-well PCR plate, with each well containing 2 µL of lysis buffer. Index sort mode was activated to record the fluorescence intensities of all markers for each individual cell. Flow cytometry standard (FCS) files from the index sort were analyzed using FlowJo software, and compensation parameters were exported as CSV tables for subsequent bioinformatic analysis. Immediately after sorting, plates containing individual cells were stored at -80°C until further processing. Following thawing, reverse transcription was performed, and the resulting cDNA was preamplified for 22 cycles. Libraries were then prepared according to the FB5P-seq protocol. The FB5P-seq data were processed to generate both a single-cell gene count matrix and single-cell B cell receptor (BCR) repertoire sequences for B cell analysis. Two separate bioinformatic pipelines were employed for gene expression and repertoire analysis, as detailed in Attaf et al. (DOI : 10.3389/fimmu.2020.00216).
identifiers.namespace: GEO
identifiers.id: GSM8465961_r1