title: GSM9034744: bone marrow-derived macrophages cells Tet2 deficient control, treatment Day 0,ChIP; Mus musculus; ChIP-Seq
library_name: GSM9034744
library_construction_protocol: Chromatin immunoprecipitation sequencing was performed on primary mouse bone marrow–derived macrophages to assess histone modifications using antibodies against H3K4me3 (Cell Signaling Technology, #5326 and #9751). Cells were crosslinked with 1% formaldehyde for 10 minutes at room temperature and quenched with 0.125 M glycine. Chromatin was extracted and sheared by sonication to 200–500 bp fragments. After elution and reversal of crosslinks, DNA was purified and used for library construction with the NEBNext Ultra II DNA Library Prep Kit (NEB). Libraries were quality-checked using an Agilent 2100 Bioanalyzer and sequenced on an Illumina NovaSeq 6000 platform (paired-end, 150 bp). Raw reads were saved in FASTQ format.
identifiers.namespace: GEO
identifiers.id: GSM9034744_r1