title: GSM8947005: Control, 4mpf, rep3; Danio rerio; RNA-Seq
library_name: GSM8947005
library_construction_protocol: Total RNA was extracted from zebrafish cardiac tissue using TRIzol reagent (Invitrogen), followed by purification with the Direct-zol RNA MiniPrep Kit (Zymo Research) according to the manufacturer's instructions. RNA-seq libraries were generated using the Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina, following the manufacturer's instructions. Briefly, library preparation was initiated by oligo-dT priming for first-strand synthesis without prior poly(A) enrichment or rRNA depletion. After RNA template removal, second-strand synthesis was carried out using random priming. Illumina-specific adapter sequences and external barcodes were introduced during library amplification. The resulting strand-specific libraries, corresponding to the sense strand of the original RNA, were sequenced as 75 bp single-end reads on an Illumina NextSeq 500.
identifiers.namespace: GEO
identifiers.id: GSM8947005_r1