title: Neg_CTRL_for_DDX59/EXOSC10
design: Negative control for knockouts of DDX59 and EXOSC10. Cell populations of lentivirally-transduced cells expressing Cas9 and non-targeting negative control sgRNA (pAVA3773) that exhibited no increase in fluorescence were FACS-isolated. 2-3 ug of total cellular RNA was extracted from FACS-isolated cells and submitted to BGI-America, San Jose, CA for cDNA library construction (without the use of poly(A) selection) and DNBSEQ paired-end sequencing (100-bp paired-end reads). Ribosomal RNA was removed using RNase H-based kit and cDNA was synthesized using random hexamer primers and reverse transcription, according to BGI protocol.
library_name: MMDM01
identifiers.namespace: SUB14871600
identifiers.id: MMDM01