title: DNA sequence of TrxA-C9-5 expressed from pcDNA plasmid
design: Random DNA sequences were cloned into the Thioredoxin backbone. Random peptides expressed from the TrxA backbone were selected that interacted with the yeast Apc10 protein via a yeast 2-hybrid screen. The TrxA-peptide construct was originally fused to an NLS, an activation domain and an HA motif. The HA-TrxA-peptide construct was subsequently cloned into the human pcDNA expression vector, as described here. The peptide sequence for C9-5 is CECLETETFHPITRHLIVP.
library_name: pcDNA-peptide C9-5
identifiers.namespace: SUB16143293
identifiers.id: pcDNA-peptide C9-5