title: GSM9215819: Follicular B cells from Cre control mice, BCR-seq, rep 2; Mus musculus; OTHER
library_name: GSM9215819
library_construction_protocol: DNA was extracted using the Machery Nagel Nucleospin DNA extraction kit according to manufacturer's instructions. V(D)J genes were amplified using the KAPA HiFi HotStart ReadyMix PCR kit (KAPA, Roche). Amplicons were purified using the Agencourt AMPure XP beads (Beckman Coulter), and second-round PCRs were performed to add Illumina NexteraXT adaptors to the IgH library. 10ng of each amplicon pool was amplified with Illumina Nextera XT adaptors to add both indexes and P5/P7 sequences for 8 cycles of PCR. Sequencing was performed using Illumina MiSeq V2 chemistry and 251base PE reads. ChIP-processed and input control samples were processed using the MGI Easy DNA FS Library preparation kit V1 (MGI Tech) and sequenced using a MGISEQ-2000RS High-Throughput Sequencing Set (PE100).
identifiers.namespace: GEO
identifiers.id: GSM9215819_r1