title: GSM9034737: 0604_2025-FB, Sample_Day1,  Tam, Tam+Rapa  and Late-rapa treated isolated hematopoietic cells bio reps 1; Mus musculus; RNA-Seq
library_name: GSM9034737
library_construction_protocol: Mice grew for 6 weeks and were processed  for HSC isolated single cell RNA sequencing (6 weeks post labeling). Processed them in two rounds (two days). Isolated bone marrow and stained with Lin, Sca, Kit, CD48, CD150 and EPCR (full HSC panel). Each treatment condition, tam-treated tam+rapa-treated and late-rapa-treated were labelled  with a specific Biolegend TotalSeq B antibody (one per mouse) to do Feature Barcoding in order to multiplex the mice (independent of the labelling). These cells were loaded in a run for performing single-cell libraries using Chromium Single Cell 3' Reagent Kits (v3).
identifiers.namespace: GEO
identifiers.id: GSM9034737_r1