title: Whole genome sequencing of Oryza sativa: leaf tissue of PB1  PBEF10 hybrid
design: Genomic DNA was extracted from young leaf tissue using the CTAB method. DNA quality and quantity were assessed using agarose gel electrophoresis and Qubit fluorometer. Paired-end sequencing libraries were prepared using the Illumina TruSeq DNA Library Preparation Kit following the manufacturers protocol. DNA was randomly fragmented, end-repaired, A-tailed, and ligated to Illumina adapters, followed by PCR enrichment and size selection (~350 bp insert size). Libraries were sequenced on the Illumina platform to generate paired-end reads.
library_name: LIB_HYB_WGS
identifiers.namespace: SUB15720235
identifiers.id: LIB_HYB_WGS