title: GSM9266364: NOD_8wk_LG_biol rep 2; Mus musculus; RNA-Seq
library_name: GSM9266364
library_construction_protocol: Excised lacrimal glands were placed in RNAlater stabilization solution (Thermo Fisher Scientific, Waltham, MA) for storage at -20˚C prior to RNA extraction. Total RNA was extracted and purified using the RNeasy Midi Kit (Qiagen, Germantown, MD) following the manufacturer's instructions. Purified RNA samples were stored at -80°C until downstream use. 100 - 200 ng of total RNA was used for the construction of the sequencing libraries using the Illumina TruSeq stranded mRNA kit, as per the manufacturer's instructions. The resulted libraries were then analyzed on an Agilent Fragment Analyzer for molar quantification and size distribution, and then pooled at equal molar for sequencing on an Illumina HiSeq 2500 sequencer using V4 chemistry and single read 50 bases format.
identifiers.namespace: GEO
identifiers.id: GSM9266364_r1