title: ChIP-seq of human ALK negative ALCL cell line (TLBR2) in parental and HELLS knockdown conditions
design: ChIP-seq of human ALK negative ALCL cell line (TLBR2) in parental and HELLS knockdown conditions
library_name: K4ME3_CTR_1903_s
library_construction_protocol: 40M cells were collected for Chromatin Immunoprecipitation (ChIP). Cells were cultured in RPMI medium supplemented with 10% FBS, 1% P/S and 20U IL-2. Functional HELLS knockdown by shRNA was induced by the treatment with Doxycycline (DOX) for 48h at final concentration of 100ng/ml. ChIP samples were purified with PCR Purification Kit (Qiagen) and eluted in 50ul of Elution Buffer. Library was obtained starting from: 1 ng for ChIPs against Histone Marks and their respective inputs, 0.4 ng for ChIP against RNA-Polymerase II and its input, 0.5 ng for ChIP against HELLS and its input. NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) protocol was followed for library preparation.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-12935:K4ME3_CTR_1903_s