title: GSM8947726: Free_add_BCKA_TGFb_3; Mus musculus; RNA-Seq
library_name: GSM8947726
library_construction_protocol: Total RNA of cells was extracted by using EASYspin RNA Mini Kit (Aidlab, RN07). Total RNA extraction from heart tissue was performed using TRIzol reagent (Vazyme, R401). The library construction process for eukaryotic transcriptome sequencing involves several key steps. Firstly, sample quality is assessed using spectrophotometry and capillary electrophoresis to ensure the purity, concentration, and integrity of RNA. Subsequently, eukaryotic mRNAs are enriched from Total RNA using Oligo(dT) magnetic beads and then fragmented. The fragmented mRNAs serve as templates for cDNA synthesis, which involves first-strand synthesis with random hexamer primers and second-strand synthesis followed by purification. After end-repairing and A-tailing, sequencing adapters are added to the cDNA fragments, which are then size-selected to yield approximately 350 bp cDNA. Finally, PCR amplification is performed to enrich the cDNA library, preparing it for sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8947726_r1