title: ChIP sequencing of T. cruzi
design: ChIP-seq ty1-H4V. ChIP-seq experiments were performed following the protocol outlined in Roson et al (2022) with certain modifications. We used 3x10^8 ty1-H4.V and untagged (expressing the Cas9) parasites (epimastigote forms) in biological triplicates. Samples were sonicated and immunoprecipitated with anti-ty-1 antibody (MA5-23513), overnight with slow rotation. The chromatin was eluted in elution buffer (50 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0, and 1% SDS) at 65C for 30 minutes. After DNA purification, ChIP and input samples were used to construct Illumina DNA Prep (M) Tagmentation libraries using IDT for Illumina DNA/RNA UD Indexes adapters, and subsequently sequenced using Illumina NextSeq 1000/2000 P2 with 100 bp reads.
library_name: JC_9chip_h4v-ty1_R2_S9_L001
identifiers.namespace: SUB15324326
identifiers.id: JC_9chip_h4v-ty1_R2_S9_L001