title: RNA sequencing of non–small cell lung cancer (NSCLC) cell line H1299 following chloroquine (CQ) treatment
design: RNA sequencing of non–small cell lung cancer (NSCLC) cell line H1299 following chloroquine (CQ) treatment
library_name: CQ-3b_p
library_construction_protocol: 3X105 H1299 cells were cultured in RPMI-1640 medium containing 10% FBS for 24 hours. The medium was then replaced with fresh RPMI-1640 supplemented with 1% FBS, 5 mM glucose, 4 mM glutamine, and 10 µM CQ for an additional 24 hours. H1299 cells were maintained in RPMI-1640 medium supplemented with 10%FBS and in humidified atmosphere with 5%CO2 at 37°C. RNA was harvested using Rneasy mini plus kit (Qiagen), 2 µg of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER Mrna-SEQ Library Prep Kit following manufactures's protocols.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16126:CQ-3b_p