title: ITS of LEO soil fungal community: S6CB_ITS
design: DNA was extracted from approximately 250 mg of homogenized soil using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) following the protocol. DNA quantity and quality were assessed using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). The fungal internal transcribed spacer region (ITS1) was amplified using the primer pair ITS1F (5'-CTTGGTCATTTAGAGGAAGTAA-3') and ITS2 (5'-GCTGCGTTCTTCATCGATGC-3'). Amplicon libraries were prepared following the same PCR conditions as the 16S libraries and sequenced on an Illumina MiSeq platform using paired-end 2  150 bp chemistry.
library_name: S6CB_ITS_paired_illumina
identifiers.namespace: SUB16050555
identifiers.id: S6CB_ITS_paired_illumina