title: 16S amplicon on soil DNA samples from pots with S. elaeagnifolium legacy cultivated with C. intybus
design: The library was prepared using the 16S Barcoding Kit 112 (Oxford Nanopore Technologies), where the full-length 16S rRNA gene was amplified via PCR using barcoded primers (27F/1492R), followed by purification with Agencourt AMPure XP beads. Equimolar amounts of barcoded amplicons (100 ng per sample) were pooled and sequenced on a MinION R9.4.1 SpotON FlowCell, using MinKNOW v3.6.5 for run control.
library_name: ONT_barcode132
identifiers.namespace: SUB15049396
identifiers.id: ONT_barcode132