title: RNA-seq of Bulinus ugandae: juvenile 6 hpe
design: Total RNA was extracted from whole bodies or pooled embryos of Bulinus ugandae using TRIzol reagent, followed by purification with the Direct-zol RNA Miniprep kit. RNA integrity was assessed using Agilent Tapestation, and quantification was performed using the AccuBlue Broad Range RNA assay. mRNA was enriched using oligo(dT)25-coupled magnetic beads with the NEBNext Poly(A) mRNA Magnetic Isolation Module. The libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, including random priming and RNA fragmentation.
library_name: 19144R-03-12
identifiers.namespace: SUB15369716
identifiers.id: 19144R-03-12