title: GSM8866233: BMDM_Input; Mus musculus; ChIP-Seq
library_name: GSM8866233
library_construction_protocol: ChIP was performed using a ChIP-IT PBMC Kit (Active Motif, 53042).  Briefly, BMDMs were harvested and fixed with formaldehyde, quenched and subjected to sonication to fragment the genomic chromatin to designated lengths. Immunoprecipitation of chromatin was performed using BRD4 (Bethyl Laboratories, A301-985A50) or H3K27ac (Active Motif, 39133). The DNA/protein complexes were washed, eluted and reverse crosslinked with proteinase K treatment. The purified DNA was harvested for further sequencing. The ChIP and input DNA were used for Illumina sequencing library construction following the standard consecutive enzymatic steps for end-polishing, dA-addition, and adaptor ligation. After a final PCR amplification step, the resulting DNA libraries were quantified and sequenced on Illumina's NextSeq 500 (75 nt reads, single end).
identifiers.namespace: GEO
identifiers.id: GSM8866233_r1