title: GSM9617410: 5-week retina, wildtype, biol rep 2; Mus musculus; RNA-Seq
library_name: GSM9617410
library_construction_protocol: Nuclei were isolated from flash frozen retinal tissue by adapting the 10X Protocol for Nuclei Isolation from Mouse Brain Tissue for Single Cell ATAC Sequencing. Briefly, for modifications, tissue was broken up by dounce before transfer to tube, and digitonin was not included in buffers for samples used with the Chromium Next GEM Single Cell 3ʹ v3.1 kit. Single-nuclei RNA-sequencing libraries were constructed according to 10X Genomics protocols for the  Chromium Next GEM Single Cell 3ʹ v3.1 kit (E16.5, P0, enhancer KO) or the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression (3-weeks), targeting 10,000 cells.
identifiers.namespace: GEO
identifiers.id: GSM9617410_r1