title: Illumina HiSeq 2500 paired-end Sequencing of KAS20000455 (KAE20000170)
design: missing
library_name: Luminal2/BMP k.o CAF RNA_TRANSCRIPTOMIC
library_construction_protocol: We extracted total RNA from1-2 x 106tumor cells using TRIzol reagent Thermo Fisher Scientific, following the manufacturer's instructions, and treated DNaseI. We constructed RNA-seq libraries using the NEXTflex Rapid Directional RNA-Seq Kit Perkin Elmer, following the manufacturer's instructions. In brief, we selected and fragmented poly-A-tailed mRNA ligated double-stranded cDNA fragments with adapters and amplified them for 9 cycles of PCR.
identifiers.namespace: KOBIC
identifiers.id: KAE20000170
identifiers.namespace: Pohang University of Science and Technology
identifiers.id: DRX601981