title: RNA-Seq of Mus musculus:adult male spleen
design: The sequencing library was constructed from splenic CD4+ T cells isolated from systemically hypoxic mice. Total RNA was extracted using the TRIzol method (Thermo Fisher Scientific). Messenger RNA (mRNA) was enriched using oligo(dT) beads. The enriched mRNA was fragmented into small pieces using fragmentation buffer and reverse transcribed into cDNA using random primers. Second-strand cDNA synthesis was performed using DNA polymerase I, RNase H, dNTPs, and buffer. The resulting cDNA fragments were purified using the QiaQuick PCR extraction kit, followed by end repair, addition of a poly(A) tail, and ligation to Illumina sequencing adapters. The ligation products were size-selected by agarose gel electrophoresis, PCR-amplified, and sequenced using Illumina HiSeqTM 2500 by Gene Denovo Biotechnology Co. (Guangzhou, China).
library_name: MM_C57BL6J_M_8w_Hypoxia3day_Mouse007
identifiers.namespace: SUB14671654
identifiers.id: MM_C57BL6J_M_8w_Hypoxia3day_Mouse007