title: GSM9617317: WT4; Mus musculus; RNA-Seq
library_name: GSM9617317
library_construction_protocol: Small intestine or colon was harvested from mice, cleaned of Payer's patches and/or luminal content and opened lengthwise. The tissue was incubated in Hank's balanced salt solution (HBSS) with HEPES, 10% bovine calf serum (BCS) and 5 mM EDTA for 20 minutes on the rotator, followed by vigorous vortexing, and another round of incubation in HBSS, and vortexing. For LP cells extraction, the tissue was further digested in 10% RPMI medium containing 1mg/mL collagenase IV (MilliporeSigma) for 40 min with vigorous shaking at 37C. LP leukocytes were purified by density gradient centrifugation using a 40% and 70% Percoll (Cytiva) gradient. Total RNA was extracted using RNeasy Mini Kits (Qiagen) per manufacturer's instructions. Total RNA integrity was determined using Agilent Bioanalyzer or 4200 Tapestation.  Library preparation was performed using SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech) per manufacturer's protocol.  Samples were indexed, pooled, and sequenced on an Illumina NovaSeq 6000.
identifiers.namespace: GEO
identifiers.id: GSM9617317_r1