title: DDX59-KO-2
design: Knockout of DDX59, replicate 2. Cell populations of lentivirally-transduced cells expressing Cas9 and gene-specific sgRNA targeting DDX59 (pAVA3317) that exhibited increased fluorescence were FACS-isolated. 2-3 ug of total cellular RNA was extracted from FACS-isolated cells and submitted to BGI-America, San Jose, CA for cDNA library construction (without the use of poly(A) selection) and DNBSEQ paired-end sequencing (100-bp paired-end reads). Ribosomal RNA was removed using RNase H-based kit and cDNA was synthesized using random hexamer primers and reverse transcription, according to BGI protocol.
library_name: MMDM06
identifiers.namespace: SUB14871600
identifiers.id: MMDM06