title: RNA-Seq of mouse brain tissue infected with Mycobacterium tuberculosis
design: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand cDNA synthesis. Libraries were generated through end repair, A-tailing, adapter ligation, size selection, amplification, and purification. Libraries were sequenced in paired-end mode on an Illumina NovaSeq X Plus platform.
library_name: MM_Brain_LTBI1
identifiers.namespace: SUB15966364
identifiers.id: MM_Brain_LTBI1