title: Amplicon seq of HAP1 (DMSO)
design: Cells expressing S.p. dCas9-VP64-p65-Rta (dCas9-VPR) were infected with the Human CRISPR Activation Pooled Library (Calabrese P65-HSF) (Sanson et al., Nat Commun. 2018, PMID: 30575746) at an MOI of 0.2 and 200x coverage. After puromycin selection, cells were cultured with DMSO for 6 days and then fixed with 70% cold ethanol and genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega) according to the manufacturer's instructions. The extracted genomic DNA was then subjected to gRNA amplification following the protocol from Olivieri and Durocher, STAR Protoc. 2021 (PMID: 33598657).
library_name: HAP1dmso
identifiers.namespace: SUB16110526
identifiers.id: HAP1dmso