title: Pooled single cell RNA-seq library of human adult PBMCs
design: The TotalSeq-C hashtag and antibody stained pool was added in replicate to the 10x Chromium Next GEM Chip K (PN-2000182) such that 180,000 B cells were loaded per sample with a well concentration of 70,000 cells per well. Gene expression (scRNA-seq), hashtag and antibody dependent tag (HTO/ADT), and targeted BCR (scVDJ-seq) libraries were processed according to the "Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) with Feature Barcode technology for Cell Surface Protein & Immune Receptor Mapping, CG000330 Rev F" User Guide using the Chromium Next GEM Single Cell 5' Kit v2, PN-1000263 (10x Genomics) and Chromium Single Cell Human BCR Amplification Kit, PN-1000253 (10x Genomics). The resultant libraries were sequenced on four NovaSeq6000 S4 200 cycle flow cells, targeting 25,000 scRNA-seq reads, 7500 scVDJ-seq and 7500 HTO/ADT reads per cell.
library_name: EXP-00723-RP1C3W5-L003_GeneExpressionLibrary
identifiers.namespace: NIAID-phs003944
identifiers.id: EXP-00723-RP1C3W5-L003_GeneExpressionLibrary