title: GSM8553321: ADN_8h_S; Homo sapiens; OTHER
library_name: GSM8553321
library_construction_protocol: After fixation, the cells were permeabilized with 0.2% Triton-X in PBS and EdU was coupled to a cleavable biotin-azide linker (Azide-PEG(3+3)-S-S-biotin) using the Click-it Kit. Purification of genomic DNA was then performed by phenol-chloroform extraction and ethanol precipitation and subjected to EdU-labelled DNA isolation. Purified EdU-labeled DNA was used for library preparation with TrueSeq ChIP Library Preparation Kit (Illumina) and high-throughput 100-bp single-end sequencing was performed on Illumina HiSeq 4000 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM8553321_r1