title: GSM9114331: Pre-clinical AD Subject 7; Homo sapiens; RNA-Seq
library_name: GSM9114331
library_construction_protocol: After brush-biopsy specimens were collected in Hibernate-A medium containing 5 µg/mL N-acetylcysteine (NAC), cells were digested for 15 min at 37 °C in a cocktail of papain (4U/mL), DNase I (10 µg/mL), 1mM EDTA, and NAC (0.05 mg/mL), then treated for 1 min with an equal volume of Accutase. The slurry was quenched with FBS, strained through a 70 µm filter, pelleted (400 g, 5 min), washed in PBS with 2% FBS, and resuspended at 1 × 10⁶ cells/mL in Hibernate-A plus 0.2 % BSA, NAC (0.05 mg/mL) 0.05 % anti-clumping reagent for immediate scRNA-seq. Samples were processed for scRNA-seq using Chromium Single Cell 3′ Paired-End, Dual-Index Library & Gel Bead Kit v4 GEMX kit (10X Genomics) targeting 20,000 cells, then sequenced using NovaSeqX (Illumina) flow cells.
identifiers.namespace: GEO
identifiers.id: GSM9114331_r1