title: GSM8465720: FH3; Mus musculus; RNA-Seq
library_name: GSM8465720
library_construction_protocol: Wild type (Shmt1+/+) and knockout (Shmt1-/-) female and male mice were randomly weaned to AIN-93G (control) diet. For the rescue arm of the study, Shmt1-/- female mice were weaned to AIN-93G diet supplemented with 8mg/kg Folic Acid (excess FA) diets. All mice were on diet for six months before surgical dissection and each diet group contained 5 mice. Initial quality control was done to assess RNA concentration, OD ratios and integrity. mRNA was isolated from 150ng total RNA using a Nextflex Poly-A Selection kit (Perkin Elmer, Waltham, MA, USA). cDNA libraries were prepared using a Nextflex Rapid Directional RNA 2.0 kit, miniaturized to 2/5 reaction volume with 12 PCR cycles and automated on a Sciclone NGSx liquid handler. The 25 libraries were pooled by equal mass. The pool was sequenced on one Illumina NovaSeq SP flowcell using the 2x100bp recipe, which yielded 940 million raw clusters, with an average of 37.6 million clusters per sample.
identifiers.namespace: GEO
identifiers.id: GSM8465720_r1