title: Exp-bound-1 (CRX781966; CRA012498)
library_name: missing
library_construction_protocol: 2 ug total RNA was fragmented by heating at 95 degreeC for 40 mins. The first strand cDNA was transcribed by SuperScript III Reverse Transcriptase (Invitrogen, 18080044) with an T7 primer (AAGCTTAGATATCTAATACGACTCACTATAGGGACCTACACGACGCTCTTCCGATC (T) 20VN) tagged with Illumina A adaptor and T7 promoter, and then the second strand was synthesized with PrimeScript Double Strand cDNA Synthesis Kit (Takara, 6111A). The libraries were amplified by in vitro transcription with RiboMAX Large Scale RNA production Systems- T7 (Promega, P1320). Next, first strand cDNA was reversed with random primers (AGTTCAGACGTGCTCTTCCGATCTNNNNNN), and then cDNAs used as template to amplify the library. The P5 and P7 adapters were linked by PCR with Platinum Taq DNA polymerase (Invitrogen, 15966025) for 12-15 cycles. Finally, 200-500 bp library products were enriched by size selection with AMPure XP Beads (Beckman Coulter, A63881). submitted_instrument_model: Illumina HiSeq X Ten
identifiers.namespace: NGDC
identifiers.id: CRX781966
identifiers.namespace: Sun Yat-sen University
identifiers.id: DRX814461