title: miRNAseq  P. americana var drymifolia interaction Fusarium sp. (Infected)
design: For miRNA sequencing, small-RNA libraries were prepared using the TruSeq Small RNA Sample Prep Kit (Illumina, San Diego, USA). From the high-quality total RNAs (5 g), small RNA fragments ranging from 1830 nt were isolated, purified, and subse-quently ligated to 3 and 5 ends adaptors. Sequentially, RNAs were reverse transcribed into cDNA and then PCR amplified (fifteen cycles). In contrast with mRNA samples, we prepared only two libraries for the miRNA sequencing, one from infected and the other for uninfected plants. An equimolar quantity of the small RNA fragments purified/isolated from each sampling point (1-, 7-, 14-, and 21 dpi) was mixed to prepare each of them. Both libraries (infected and uninfected) were tested by gel electrophoresis, and bands corre-sponding to miRNA insertion were cut and eluted. After ethanol precipitation and wash-ing, the miRNA libraries were quantified and sequenced using the NextSeq500 (Illumina, San Diego, USA) platform (single-end reads, 50 cycles).
library_name: miRNAs_infected_1
identifiers.namespace: SUB14665593
identifiers.id: miRNAs_infected_1