title: HiFi sequencing of Anticarsia gemmatalis: adult female light 04
design: High molecular weight (HMW) DNA extraction was performed from the  using the Qiagen MagAttract HMW DNA Kit. DNA shearing was performed using the Diagenode Megaruptor 3 with the 20 kb fragment protocol. The sheared DNA was prepared for PacBio sequencing using the SMRTBell Express Template kit 3.0. The library was size-selected with beads to remove library molecules less than 3 kb in length to generate the final libraries for sequencing. Sequencing was performed on a Revio System. To target HiFi reads, the library was sequenced using a 30 hour movie time on one Revio cell. Raw subreads were converted to HiFi data by processing with CCS to call a single high quality consensus sequence for each molecule, using a 99.5% consensus accuracy cutoff using SMRTLink software v.10.1.
library_name: Light_04_HiFi_bc2040
identifiers.namespace: SUB16112282
identifiers.id: Light_04_HiFi_bc2040