title: GSM8465849: CRAMP1-KO + GFP-CRAMP1-∆SANT cells, RNA, exp2, rep1; Homo sapiens; RNA-Seq
library_name: GSM8465849
library_construction_protocol: RNA was extracted using the RNeasy Mini Kit (Qiagen X) and quality checked on a TapeStation. 500 ng total RNA was used for the next steps. Ribosomal RNA was depleted using the NEBNext rRNA depletion kit v2 (New England Biolabs, E7405). Libraries were constructed using the NEBNext Ultra II Directional RNA library prep kit (New England Biolabs, E7760) with the NEBNext Multiplex Oligos for Illumina (New England Biolabs, E6440). Libraries were amplified with 8 PCR cycles, size selected using AMPure XP beads (Beckman Coulter, A63881) and quality was assesed using the HS D1000 kit on a TapeStation (Agilent).
identifiers.namespace: GEO
identifiers.id: GSM8465849_r1