title: GSM9179287: MEF_m6Aseq_IP; Mus musculus; OTHER
library_name: GSM9179287
library_construction_protocol: RNAs were extracted using TRIzol reagent poly(A)-RNA was purified using Dynabeads mRNA DIRECT purification kit (Thermo Fisher), and was then immunoprecipitated against an m6A antibody (Synaptic Systems) bound to Protein G Dynabeads (Thermo Fisher), eluted by competition with free m6A (Sigma). The precipitated RNAs were reverse transcribed, amplified, tagmented and indexed using Smart-Seq2 protocol.
identifiers.namespace: GEO
identifiers.id: GSM9179287_r1