title: GSM9069605: Parental cell line, mouse anti-FLAG, replicate 1; Homo sapiens; OTHER
library_name: GSM9069605
library_construction_protocol: CUT&Run was performed as previously described (dx.doi.org/10.17504/protocols.io.zcpf2vn), using 0.5x10^6 cells per antibody, 0.02% digitonin and pA/G-MNase digestion (30 minutes on ice). After phenol:chloroform extraction DNA was purified with home-made serapure beads (Doi:10.1101/gr.128124.111). NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®, adapted for short fragments (dx.doi.org/10.17504/protocols.io.bagaibse); adaptors diluted 1:20; 11 PCR cycles
identifiers.namespace: GEO
identifiers.id: GSM9069605_r1