title: RNA-seq comparing the WT mouse hearts versus Dhx36 cKO hearts
design: RNA-seq comparing the WT mouse hearts versus Dhx36 cKO hearts
library_name: KO5_s
library_construction_protocol: whole hearts (including atria) were surgically isolated from 2- to 3-week-old WT or Dhx36myh6 mice. The isolated organs were individually submerged into 1 mL TRIsure buffer (Meridian Bioscience #BIO-38032), immediately snap-frozen in liquid N2, and stored at -80ºC. Tissue homogenization was performed using the MagNA lyser system (Roche). total RNA was isolated from 4-6 hearts per genotype. The RNA was then pooled and purified on Qiagen-RNA-clean columns (Qiagen #74204). Library construction was performed with TruSeq™ RNA Sample Prep Kit v2 (Illumina)
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14440:KO5_s