title: Metagenomic Investigation of the Whitefly Gut Microbiome to Assess the Impact of Weather and Seasonality on Whitefly Population Dynamics, Microbial Communities, and Virus Transmission in Tomato Crops
design: For metagenomic analysis, the 16S rRNA gene was amplified from genomic DNA via polymerase chain reaction (PCR) using universal primers. The PCR amplification protocol consisted of an initial denaturation at 94C for 2 minutes, followed by 29 cycles of denaturation at 94C for 45 seconds, primer annealing at 55C for 1 minute, and extension at 72C for 2 minutes, with a final extension step at 72C for 10 minutes. The PCR products were resolved by electrophoresis on a 0.8% agarose gel. Sequencing of the V4 region of the 16S rRNA gene from whitefly samples was performed using Illumina MiSeq 2x250 base pair paired-end amplicon sequencing technology.
library_name: ONKOL
identifiers.namespace: SUB14752124
identifiers.id: ONKOL