title: GSM8947457: human PBMC a24-CMV exposed to mutated antigen; Homo sapiens; RNA-Seq
library_name: GSM8947457
library_construction_protocol: PBMC cultured for two weeks were havested and frozen at a concentration of 1x10^6/ml, then were thawed at a viability > 80% for library preparation. For cDNA library construction, Chromium Next GEM Single Cell 3' GEM kit v3.1, Gel Bead kit v3.1, Chip G kit, Library constructuion kit, Dual Index TT set (10x Genomics) were used following manufacture's instructions. Quality controls of cDNA and Library were peformed using Agilent TapeStation. GEX-PCR cycles for cDNA and Library were 11 and 12, respectively.
identifiers.namespace: GEO
identifiers.id: GSM8947457_r1