title: GSM9035002: NTDsA; Mus musculus; ATAC-seq
library_name: GSM9035002
library_construction_protocol: On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope and the brain were used for experiments.The brain tissue was digested into a single-cell suspension, and 100,000 cells were used for experiments according to Hyperactive ATAC-Seq Library Prep Kit for Illumina(TD711-01). Purified transposed DNA using Vazyme DNA Clean Beads (Catalog #TD903)，PCR Amplification：72°C for 5 min (gap repair) ；98°C for 30 sec ；12 cycles: 98°C 10 sec, 63°C 30 sec, 72°C 30 sec ；72°C for 5 min (final extension) .Library Purification & QC:Purified with Vazyme DNA Clean Beads (1.0× ratio).Quantified with Qubit dsDNA HS Assay.
identifiers.namespace: GEO
identifiers.id: GSM9035002_r1