title: RNA-Seq of RKO (human) cells treated with Tal 2.5uM for 10h (rep1)
design: RKO cells were treated with CPT (2.5M) with or without PARPi (Talazoparib, 2.5M) and ATMi (10 M) for 10 h, and total RNA was extracted using the Quick-RNA MiniPrep Kit (Zymo #11328). Poly(A) RNAs from five micrograms of total RNA for each sample were then selected using NEBNext Poly(A) mRNA magnetic isolation module (#E7490L) and were then used to construct the sequencing library using Invitrogen Collibri Stranded RNA Library Prep Kit (Thermo Fisher Scientific, #A39114096), according to the manufacturers protocols. Libraries were amplified using 12 PCR cycles. Library size distributions were measured using a BioAnalyzer and quantified through qPCR. Libraries were sequenced on a NovaSeq X Plus platform.
library_name: Tal2.5uM_10h_rep1
identifiers.namespace: SUB15792205
identifiers.id: Tal2.5uM_10h_rep1