title: apr047_hic
design: Quality of the Hi-C sequencing libraries were determined using D5000 ScreenTape and Qubit 1X dsDNA High Sensitivity (HS) assay. The purified library was stored at 20C in a DNA low-binding tube until ready to use for sequencing. Each library was normalized to 3nM in 35 ?L. Samples with 6 different Illumina adapters were pooled into one pool, resulting in a total volume of 210 ?L. The pool was thoroughly vortexed and spun down. Subsequently, 150 ?L of this mix was transferred to a new 1.5 mL tube, and 37 ?L of 0.2N HP3 was added. After vortexing and spinning, the mixture was incubated at room temperature for 8 minutes. Following this, 38 ?L of 400 mM Tris-HCl, pH 8.0, was added, and the mixture was vortexed and spun down again. Finally, 225 ?L of the mix was added to the library tube and loaded onto the Novaseq 6000 for sequencing.
library_name: apr047
identifiers.namespace: SUB14673253
identifiers.id: apr047