title: GSM8517998: RNA from microtom seeds treated with osmopriming and MeJA 2 mM (PEG 8000 -1MPa) for 48h rep3; Solanum lycopersicum; RNA-Seq
library_name: GSM8517998
library_construction_protocol: RNA samples were extracted from the same seed lot used to produce exudates following the seed priming treatment with or without elicitors using the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany) with lysis buffer containing 1% of polyvinylpyrrolidone (PVP-40) followed by incubation at room temperature for 10 minutes. RNA quantity and quality was measured using a NanoDrop ND‐1000 (NanoDrop Technologies, Wilmington, DE, USA) and a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA), respectively. All samples showing 260/280 and 260/230 absorbance ratio >1.8, RNA Integrity Number, RIN>7 and 28S/18S>1.7 were sent to Beijing Genomics Institute (https:// www.bgi.com) (Hong Kong, China) for library preparation and sequencing on BGISEQ-500 platform, generating a minimum of 20M reads of 100 bp per sample (20M PE100). PolyA enrichement library done by BGI
identifiers.namespace: GEO
identifiers.id: GSM8517998_r1