title: Gene-seq of mus musculus:sample7
design: Data Evaluation and Quality Control: Quality assessment of raw sequencing data was performed using FastQC. Illumina sequencing data were quality-trimmed using Trimmomatic to obtain relatively accurate valid data.  Genome Assembly: SPAdes was used for reference-free or reference-based assembly of next-generation sequencing data. GapFiller was applied to fill gaps in the assembled contigs. PrInSeS-G was utilized for sequence correction to fix base errors and small insertion-deletion mutations during assembly.  Genomic Component Analysis: Prokka was used to predict gene elements, including genes, tRNA, rRNA, etc. RepeatMasker was employed to identify repetitive sequences in the genome.  Gene Annotation: NCBI Blast+ was used to align gene protein sequences against multiple databases (CDD, KOG, COG, NR, NT, PFAM, Swissprot, TrEMBL, etc.) to obtain functional annotation information. GO functional annotation information was derived from the annotation results of genes against Swissprot and TrEMBL. KEGG annotation information for genes was obtained using KAAS.
library_name: YNDF2-6
identifiers.namespace: SUB15370637
identifiers.id: YNDF2-6