title: GSM9264014: PBMC, GCSF+ISO, replicate 1; Homo sapiens; RNA-Seq
library_name: GSM9264014
library_construction_protocol: Peripheral blood mononuclear cells (PBMCs) were isolated from 6mL of ACD whole blood, collected at baseline and after a 20-min ISO infusion, by density gradient centrifugation (Cytiva Ficoll-PaqueTM, Fisher Scientific, Hampton, NH) and cryopreserved for later analysis. Isolated PBMCs were then resuspended in a PBS/RNAlater solution and delivered to the University of Arizona Genetics Core for single cell RNA sequencing (scRNAseq) analysis using the 10x Genomics platform. Targeted Gene Expression libraries generated from Chromium Single Cell 5' Gene Expression and Chromium Single Cell V(D)J libraries comprised standard Illumina paired-end constructs which begin with P5 and end with P7. The gene expression libraries were quantified, normalized, pooled, and sequenced on an Illumina NextSeq500 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM9264014_r1