title: GSM9083274: BM_oxa_5; Mus musculus; RNA-Seq
library_name: GSM9083274
library_construction_protocol: For Kidney samples, total RNA was extracted with PureLink RNA mini kit following manufactors' instructions. For bone marrow, RNA was extracted with Trizol followed by column-based purification using PureLink column. Library preparation was performed with 500ng to 1ug of total RNA. Ribosomal RNA was removed by an RNase-H method using RiboErase kits (Kapa Biosystems). mRNA was then fragmented in reverse transcriptase buffer and heating to 94 degrees for 8 minutes. mRNA was reverse transcribed to yield cDNA using SuperScript III RT enzyme (Life Technologies, per manufacturer's instructions) and random hexamers. A second strand reaction was performed to yield ds-cDNA. cDNA was blunt ended, had an A base added to the 3' ends, and then had Illumina sequencing adapters ligated to the ends. Ligated fragments were then amplified for 12-15 cycles using primers incorporating unique dual index tags.
identifiers.namespace: GEO
identifiers.id: GSM9083274_r1