title: GSM8520359: LMPP_H_250123_L1; Mus musculus; RNA-Seq
library_name: GSM8520359
library_construction_protocol: cells were sorted from freshly isolated mouse E14.5 foetal livers from a mouse line that has doxycyline-inducible KMT2A-MLLT3 expression; DOX was given at E12.5 and embryos retireved 2 days later for foetal liver dissection; single cell preparations were stained with cell surface markers for LMPPs (Lin-Sca1+ckit+CD45+Flt3+) with or without CSF1R cell surface expression and the two LMPP populations (CSF1R+/-) directly sorted into lysis buffer. RNA was extracted with RNAeasy micro kit (Qiagen).  0.5ng of total RNA was used to prepare the libraries. the libraries were prepared using the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB #E6420) according to the provided protocol.
identifiers.namespace: GEO
identifiers.id: GSM8520359_r1