title: GSM9473524: v4 ko CD8T-1; Mus musculus; OTHER
library_name: GSM9473524
library_construction_protocol: Liver tissues enriched for metastatic MC38 melanomas were harvested from WT or Vsig4-/- mice, cut into small pieces and digested with 0.5 mg/mL collagenase IV (Sigma) and 5 U/mL DNase I (Sigma) for 20 mins at 37℃ under shaking. The cell suspension was filtered through a 70 µm cell strainer and centrifugated at 400g for 5 mins at 4℃, followed by lysis of red blood cell inn ACK buffer (Biolegend).DAPI-CD45+CD3+CD8+ T cells  were sorted by FACS and further processed for bulk TCR-seq. RNA libraries for TCR-seq were prepared using Evo M-MLV Kit Ver.2 AG11728 kit  following manufacture's protocols.
identifiers.namespace: GEO
identifiers.id: GSM9473524_r1