title: D12-3 (CRX799443; CRA012775)
library_name: missing
library_construction_protocol: Transcriptome library construction involves the purification and fragmentation of poly(A) RNA, followed by reverse transcription to generate cDNA, synthesis of U-labeled second-stranded DNAs, and modification of blunt ends with an A-base for adapter ligation. The adapter-ligated fragments undergo size selection, followed by treatment with heat-labile UDG enzyme and PCR amplification to create the final cDNA library with an average insert size of 300+/-61 bp.
identifiers.namespace: China Agriculture University
identifiers.id: DRX816816
identifiers.namespace: NGDC
identifiers.id: CRX799443