title: GSM9440163: PTPN11 N308D, Day 14 Endocardial; Homo sapiens; RNA-Seq
library_name: GSM9440163
library_construction_protocol: Gel-bead in emulsions (GEMs) were generated on the Chromium controller sample chip (10X Genomics) using live cell samples with an input of ~10,000 cells. Barcoded cDNA was then extracted from the GEMs by post-GEM RT-cleanup and amplified for 12 cycles. Amplified cDNA was fragmented and subjected to end-repair, poly A-tailing, adapter ligation, and 10X-specific sample indexing following the maufacturer's protocol. Libraries were quantified with Bioanalyzer (Agilent) and QuBit (ThermoFisher) analysis.
identifiers.namespace: GEO
identifiers.id: GSM9440163_r1