title: RNAseq  P. americana var drymifolia interaction Fusarium sp. (Infected, dpi 14, replicate 1)
design: For mRNA sequencing, high-quality RNA (500 ng) was processed with the TruSeq RNA Sample Prep Kit version 2.0, following the manufacturer's instructions. Briefly, using poly-T oligo-attached magnetic beads, mRNA was poly-A-selected to deplete the riboso-mal RNA fraction. The cleaved mRNA fragments were reverse transcribed into cDNA, and once the ends were repaired, the Illumina dual-index adapters were ligated onto them. Each library (sixteen in total, i.e., two independent biological replicates from infected and uninfected plants at 1-, 7-, 14-, and 21 dpi) was independently labeled with a specific multiple. 250350 bp fragments were excised with Agencourt AMPure XP beads (Beck-man Coulter) and amplified by PCR. After purification, the libraries' quality was checked using Bioanalyzer 2100 (Agilent). mRNA sequencing was then performed on a NextSeq500 (Illumina) platform to generate 150 bp paired-end reads (300 cycles).
library_name: inf-dpi14_1
identifiers.namespace: SUB14665593
identifiers.id: inf-dpi14_1