title: Amplicon sequencing of quinoprotein glucose dehydrogenase gene(gcd) from soil samples in Horqin Sandy Land
design: Soil samples were collected from four successional stages (shifting sand dune, early-stage, mid-stage, and final-stage) in the Horqin Sandy Land, Inner Mongolia, China (4302N, 11939E). At each stage, three replicate plots (20 m  20 m) were established. Topsoil (010 cm) was collected from 20 random points per plot, pooled, sieved (2 mm and 1 mm), and stored at -84 C. Total genomic DNA was extracted from each soil sample. The target functional genes (e.g., carbon-fixation genes) were amplified by PCR using specific primers. Amplicons were purified and subjected to paired-end (PE) sequencing on an Illumina platform (NovaSeq or MiSeq) according to the manufacturers protocols. The library was prepared as amplicon libraries, and raw sequencing data were generated in FASTQ format.
library_name: EP2_gcd
identifiers.namespace: SUB16080889
identifiers.id: EP2_gcd