title: virus metagenome sequencing from California mussel (Mytilus californianus) : hepatopancreas
design: To generate sufficient material for sequencing, a random reverse transcription/amplification protocol was used to amplify both viral DNA and RNA. Briefly, 5 L of nucleic acids were mixed with primer-A and heated, followed by reverse transcription using SuperScript III and amplification with Sequenase DNA Polymerase. The resulting PCR product was purified and eluted, and no specific concentration was applied before barcoding. DNA was then purified using AMPure XP SPRI beads. For library preparation, 12 L of cDNA was used with the Ligation Sequencing gDNA Native Barcoding Kit, and the final library size was assessed with the Agilent 2200 TapeStation. The library was sequenced on a PromethION flow cell with R10.4.1 chemistry, basecalled using dorado, and demultiplexed on a high-performance computing system.
library_name: M11
identifiers.namespace: SUB15274196
identifiers.id: M11