title: GSM8471389: Outer Bowel Wall; Homo sapiens; OTHER
library_name: GSM8471389
library_construction_protocol: To accommodate the size constraints of the Visium chip, we harvested a 1cm-thick wedge from an intestinal stricture and divided it into two sections: 1) mucosa through the muscularis propria (Inner Bowel Wall), and 2) the muscularis propria and surrounding fat (outer bowel). The sample was placed in a 1cm x 1cm block (TissueTek) with pre-chilled OCT and processed according to the Visual Spatial Protocol-Tissue Preparation Guide by 10X Genomics. Briefly, we flash froze tissue samples in OCT using a Thermo Scientific Thermo-Flask benchtop container filled with liquid nitrogen and a small, round, metal bowel with 1-inch height worth of 2-methylbutane. A pair of needle-drivers was used to maneuver the 2-methylbutane bowl in and out of the Thermo-Flask. The OCT block was placed in the 2-methylbutane bowl and lowered into the liquid nitrogen until the tissue was completely frozen. The tissue was then placed on dry ice and transferred to a -80 freezer. Tissues were cyrosectioned at -20C onto gene expression slides. The Gene Expression Slide & Reagent kit was followed per the 10X Genomics protocol and used to produce sequencing libraries.
identifiers.namespace: GEO
identifiers.id: GSM8471389_r1