title: RNA-Seq of mus musculus: adult female whole blood
design: Whole blood samples were collected from mice 28 days after the initial immunization.About 2g total RNA of each sample were used for TCR sequencing library preparation using KC-DigitalTM Stranded TCR-seq Library Prep Kit for Illumina 150 (Seqhealth Technology Co., Ltd., Wuhan, China, Cat. No. DT0813-02) following the manufacturers instruction. The kit eliminates duplicationbias in PCR and sequencing steps, by using unique molecular identifier (UMI) of 8 random bases to label the pre-amplified cDNA molecules.The library products corresponding to 250-500 bp were enriched, quantified and finally sequenced on NovaSeq (Illumina).The sequence was mapped to the international ImMunoGeneTics (IMGT) database. And using MiXCR software (version 3.0.3) to obtain V , D, and J fragments, rearrangement, and CDR3 sequences.
library_name: 7
identifiers.namespace: SUB14695550
identifiers.id: 7