title: GSM9003659: Ton_FRC_10_ng_mL_48hr; R1; Homo sapiens; RNA-Seq
library_name: GSM9003659
library_construction_protocol: Tonsillar fibroblastic reticular cells (tFRCs) were isolated from fresh tonsils. The cells were passaged for six generations in Insulin-Transferrin-Selenium (Thermo Fisher) containing Dulbecco's Modified Eagle Medium (DMEM, Corning) supplemented with 10% fetal Bovine serum (FBS) and Penicillin (100 units/mL)-streptomycin (100 µg/mL). The tFRCs were stimulated with 10 ng/mL and 50 ng/mL TGFB (Peprotech). The cells were harvested after 48 hours post treatment and subjected to bulk RNA sequencing. RNA was extracted using RNeasy micro kit (QIAGEN) according to the manufacturer's protocol after washing the cells with PBS, followed by library preparation for RNAseq. Illumina NovaSeq 6000 platform, using the PE100 S4 flow cell for paired-end sequencing with a 200-cycle v1.5 kit
identifiers.namespace: GEO
identifiers.id: GSM9003659_r1