title: GSM9579609: articular chondrocytes, Control2; Homo sapiens; RNA-Seq
library_name: GSM9579609
library_construction_protocol: Total RNA was isolated with the RNeasy kit with gDNA eliminator (Qiagen) according to the manufacturer's protocol and subjected to rRNA depletion. Was checked for quality and integrity on the Agilent 2100 Bioanalyzer (Santa Clara, CA, United States) via 2100. An Expert Eukaryotic Total RNA Nano chip was used according to the manufacturer's protocol. The mRNA sequencing library was generated using the TruSeq mRNA Sample Preparation Kit (Illumina, Eindhoven, Netherlands). In short, mRNA was enriched using magnetic beads coated with poly(dT), then fragmented. The fragmented mRNA-enriched samples were subjected to cDNA synthesis by reverse transcriptase, followed by dA-tailing and ligation of specific double-stranded bar-coded adapters. Next, the library was amplified, followed by a cleanup. The library sizes were determined using an Agilent 2100 Bioanalyzer with a DNA 1000 chip, according to the manufacturer's protocol. Pooled libraries consisting of equal molar samples were sequenced on a high-output 75-bp single read on the NextSeq500 (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM9579609_r1