title: GSM9178712: Chimeric mouse diaphragm experimental dataset, Day2, gene expression; Mus musculus; RNA-Seq
library_name: GSM9178712
library_construction_protocol: Diaphragm macrophages were identified as CD45-positive (CD45.1 or CD45.2), CD11b-positive, and F4/80-positive cells, and negative for SiglecF and CD11c. For the Chimeric mouse diaphragm validation dataset, Cell Multiplexing Oligos (CMO, 10x Genomics) were applied to FACS-sorted CD45.1 and CD45.2 macrophages to distinguish donor and recipient origins. In Chimeric mouse diaphragm experimental datasets, CMOs were applied to unstained muscle cell suspensions prior to sorting to trace cells from different mice. All macrophage sorting was performed in PBS supplemented with 20% fetal bovine serum (FBS) to preserve viability and cell integrity. The Single Cell 3' mRNA and CMO barcode library preparation was conducted strictly following 10x Genomics protocol CG000388.
identifiers.namespace: GEO
identifiers.id: GSM9178712_r1