title: miRNA-seq of Aphis craccivora
design: After total RNA was extracted by TRIzol reagent kit (Invitrogen, USA), the RNA molecules in a size range of 18-30 nt were enriched by polyacrylamide gel electrophoresis. Then the 3 adapters were added and the 36-44 nt RNAs were enriched. The 5 adapters were then ligated to the RNAs as well. The ligation products were reverse transcribed by PCR amplification and the 140-160 bp size PCR products were enriched to generate a cDNA library and sequenced using Illumina Hiseq 2505 by LC-BIO (Hangzhou, China).
library_name: T3
identifiers.namespace: SUB15243352
identifiers.id: T3