title: RNA-seq of mouse neurons in mono-culture and in co-culture with neurons, with application of Nrf2 activator tBHQ.
design: RNA-seq of mouse neurons in mono-culture and in co-culture with neurons, with application of Nrf2 activator tBHQ.
library_name: BN5_p
library_construction_protocol: "Neurons and astrocytes were cultured as described in Bell et al., \""Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2\"", Nature Communications 6: 7066 (2015)." Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated ± tBHQ (10 µM) for 8h. At this point RNA was extracted and subject to RNA-seq analysis. RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's protocol (Illumina).
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-5688:BN5_p