title: GSM8637405: CR-Mlg086; Mus musculus; RNA-Seq
library_name: GSM8637405
library_construction_protocol: Tissues were rinsed with PBS, cut into 1 mm3 pieces, and transferred to 5 ml of digestive solution (DMEM High Sugar (Sigma-Aldrich) with the addition of L-glutamine-penicillin-streptomycin (100 U/ml) (Sigma-Aldrich), 20 uL of DNase (Quiagen) (75 U/ml), 2 mL of Collagenase V (2 %) (Sigma-Aldrich) and Dispase II (2. 5 U/mL) (Gibco)) in a water bath (37 °C) for 45 min.   Translated with www.DeepL.com/Translator (free version) For scRNA sequencing, libraries were generated and prepared for single-cell suspensions using a 10X Chromium Chip Single-Cell Library Generator (10X Chromium 3'solution (V2 kit)) according to the manufacturer's instructions, with the goal of capturing 5,000-1,000 cells/channel. Sequencing was performed using the Illumina HiSeq 4000 platform.
identifiers.namespace: GEO
identifiers.id: GSM8637405_r1