title: 16s rDNA of anaerobic fermentation sludge
design: Total microbial genomic DNA was extracted from anaerobic fermentation sludge using the E.Z.N.A. soil DNA Kit (Omega Bio-tek, Norcross, GA, U.S.). The number of microorganisms was determined using an ABI7300 fluorescence quantitative PCR instrument (Applied Biosystems, USA), and qPCR analysis was performed on the V3 region of the bacterial 16S rRNA gene. The primers used in qPCR were 357F (CCTACGGGAGGCAGCA) and 518R (ATTACCGCGGCTGCTGG). Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina Nextseq2000 platform (Illumina, San Diego,USA) according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China).
library_name: S5
identifiers.namespace: SUB15275899
identifiers.id: S5