title: GSM8947468: RNASeq_PA2_E11.5_WT_2; Mus musculus; RNA-Seq
library_name: GSM8947468
library_construction_protocol: Dissected tissue (i.e. FNP, Mx, Md and PA2) from E11.5 Wnt1::Cre;ROSARFP and Ezh2cKO embryos were kept in 1× PBS on ice, then treated with 0.5% trypsin/1× EDTA at 37 °C for 10 minutes and immediately put on ice. The tissue was rinsed once in ice-cold 1× DMEM/10% FBS, followed by two washes in ice-cold 1× DMEM. Tissue was dissociated by pipetting. CNCCs were filtered and collected by FACS. After sorting, CNCCs were pelleted by centrifugation for 5 minutes, 1000 rpm at 4°C. Total RNA was extracted using the Single Cell RNA Purification Kit (NORGEN, 51800) with genomic DNA digestion using an RNase-Free DNase I Kit (NORGEN, 25710) according to the manufacturers' protocol. For each sample, three independent biological replicates were prepared. For sequencing of total RNA, unstranded RNA-Seq libraries were prepared using Illumina TruSeq RNA Library Prep Kit v2 according to manufacturers' instructions. Sequencing was performed on an Illumina HiSeq 2500 machine (50 bp read length, single-end).
identifiers.namespace: GEO
identifiers.id: GSM8947468_r1