title: GSM8865024: Ah root, control, biol rep1; Arabidopsis halleri subsp. gemmifera; RNA-Seq
library_name: GSM8865024
library_construction_protocol: Root RNA was extracted in 1 mL of TRIzol reagent. Samples were mixed briefly and incubated for 30 min at room temperature. Subsequently, chloroform (200 μL) was added to the sample and the mixture was vigorously shaken for 30 s. The samples were centrifuged at 15,000g at 4°C for 15 min, and the upper aqueous phase was carefully transferred to a new tube. RNA was precipitated by the addition of 0.5 mL of isopropanol and incubation at −80°C for 30 min. Following centrifugation at 15,000g at 4°C for 15 min, the resulting pellet was washed twice with 75% ethanol. The residual ethanol was evaporated in a chemical fume hood for 10 min. RNA was redissolved in 30 μL of diethyl pyrocarbonate-treated water. Libraries were prepared using TrueSeq Illumina kit, following manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8865024_r1