title: ddRAD of Porcellio spinicornis: adult pereion and leg tissues
design: Library preparation followed the ddRADseq approach of Peterson et al. (2012) and was performed by the UAlberta Molecular Biology Service Unit. Preparation followed MacDonald (2020) with the following modifications: (1) the restriction enzymes were EcoR1 and Pst1; (2) all sixteen i5 adaptors were used, but subpools were made with 10 microlitres of eight sample ligations instead of 5 microlitres of 16 samples (step 3); (3) purification of the final pool was done with a 1:1 ratio of Seramag Select beads following the manufacturer's protocol; 4) size range of the final pool was 400-2000 (average 1000 bp). Sequencing was performed using a NextSeq 500 (Illumina) and a 75-cycle high output kit. Demultiplexing was performed in the process_radtags program in Stacks v2.64 (Rochette et al. 2019). Reads were demultiplexed and filtered for quality with Stacks v 2.64 (Rochette et al. 2019) with further filtering in fastP v 0.23.1 (Chen et al. 2018) to remove enzyme cut sites and Illumina adaptor sequences. Final cleaned sequences are 63 bp long.
library_name: 161-1
identifiers.namespace: SUB15660909
identifiers.id: 161-1