title: Transcriptomic profiling of NK-92, CAR NK-92, and NR3C1-knockout CAR NK-92 cells following co-culture with CEACAM5⁺ A549 lung cancer cells
design: Transcriptomic profiling of NK-92, CAR NK-92, and NR3C1-knockout CAR NK-92 cells following co-culture with CEACAM5⁺ A549 lung cancer cells
library_name: Sample 12_p
library_construction_protocol: NK-92, CAR NK-92, hydrocortisone-treated CAR NK-92, NR3C1-knockout CAR NK-92, and hydrocortisone-treated NR3C1-knockout CAR NK-92 cells were co-cultured with CEACAM5⁺ A549 target cells for 16 hours. After co-culture, NK-92–derived cells were collected by FACS sorting. Sorted cell pellets were immediately processed for RNA extraction. Total RNA was extracted using the Qiagen RNA extraction kit following the manufacturer's instructions, including optional on-column DNase digestion. RNA concentration and purity were assessed using NanoDrop. High-quality RNA was used for subsequent library preparation by Novogene.  RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16373:Sample 12_p