title: GSM8465834: CRAMP1-KO + GFP-CRAMP1-∆SANT cells, CUT&RUN, rep3; Homo sapiens; OTHER
library_name: GSM8465834
library_construction_protocol: Cells were permeabilised with NaCl and Triton-X before incubating with indicated antibodies and subsequently with MNase-pAG. Spike-in DNA was added before releasing and purifying the fragments. Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, E7760) with the Multiplex Oligos for Illumina (New England Biolabs, E6440). DNA was amplified with 12 PCR cycles. PCR products were purified and size selected using AMPure XP beads (Beckman Coulter, A63881) ). The quality was checked on a TapeStation 4200 using D1000 HS kit.
identifiers.namespace: GEO
identifiers.id: GSM8465834_r1