title: Intestinal of zebrafish from H treatment group, biological replicate 3, technical replicate 3
design: RNA-seq analysis of zebrafish (Danio rerio) intestinal tissue to investigate histamine-induced intestinal injury and the restorative effects of Lactobacillus reuteri postbiotics. Total RNA was extracted from intestinal tissues using TRIzol reagent. mRNA was enriched by PolyA selection using NEBNext Poly(A) mRNA Magnetic Isolation Module. cDNA libraries were constructed with NEBNext Ultra II RNA Library Prep Kit for Illumina. Sequencing was performed on the Illumina NovaSeq X Plus platform, generating 2150 bp paired-end reads. Data will be used for differential gene expression analysis and pathway enrichment to elucidate molecular mechanisms of intestinal inflammation and repair.
library_name: ZF_I_H_Rep3
identifiers.namespace: SUB15991178
identifiers.id: ZF_I_H_Rep3