title: GSM9035120: KO_toti; Homo sapiens; RNA-Seq
library_name: GSM9035120
library_construction_protocol: 4CL and me4CL cells were lysed and the released RNA was barcoded through reverse transcription in individual gel beads in emulsion (GEMs). Reverse transcription was performed on a S1000 Touch Thermal Cycler (Bio-Rad) at 53 °C for 45 min, followed by 85 °C for 5 min, and then held at 4 °C. The cDNA was generated and then amplified, and quality-assessed using the Agilent 4200 system. According to the manufacturer's instructions, scRNA-seq libraries were constructed using the Single Cell 3′ Library and Gel Bead Kit V3.1.
identifiers.namespace: GEO
identifiers.id: GSM9035120_r1