title: GSM8637958: E12.5 Wnt9b Knockout out mouse 1 mesonephros; Mus musculus; RNA-Seq
library_name: GSM8637958
library_construction_protocol: RNA was harvested using PicoPure RNA Isolation Kit from Applied Biosystems cat# 12204001 Upond recieval of samples, a quality check is performed to check for RNA amount and purity. Then the library is contruction using polyA enrichment to capture Poly(A) and remove rRNA. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Upon fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H.  Once cDNA synthesis is finished, 250-300bp insert cDNA library was prepared. The library was ready once overhangs were converted to ends, purified and libraries were assesed for quality and 150bp paired-end reads were generated.
identifiers.namespace: GEO
identifiers.id: GSM8637958_r1