title: GSM8865710: Pkd1 null MEK cells  repeat 3; Mus musculus; RNA-Seq
library_name: GSM8865710
library_construction_protocol: Libraries were prepared using the Illumina TruSeq Stranded Total RNA (Cat. # RS-122-2301) or mRNA (Cat. # RS-122-2101) kit according to the manufacturer's protocol starting with 1 μg total RNA. Briefly, the data processing step is rRNA-depleted RNAs (Total RNA kit) or purified mRNAs (mRNA kit) were fragmented and converted to cDNA with reverse transcriptase. The resulting cDNAs were converted to double stranded cDNAs and subjected to end-repair, A-tailing, and adapter ligation. The constructed libraries were amplified using 8 cycles of PCR. Total RNA was extracted using the RNeasy Plus Mini Kit (QIAGEN).
identifiers.namespace: GEO
identifiers.id: GSM8865710_r1