title: Fungal (ITS) microbiome of soil: soil with added MilliQ water
design: DNA was extracted from 0.25 g of fresh (frozen) soil using the DNeasy PowerSoil Pro Kit (QIAGEN N.V., Germany) following the manufacturers protocol. Fungal community composition was assessed by amplifying the ITS region using primer pair CS1_ITS1F and CS2_ITS2R (Core Research Resource Center, University of Illinois). Library preparation for sequencing involved two rounds of PCR. The first PCR reaction mixture (20 L total volume) consisted of 1 L of normalized template DNA, 0.2 L of DreamTaq DNA Polymerase (5 U/L), 2 L of 10 DreamTaq Buffer, 2 L of 1 mM dNTPs, 0.6 L each of forward and reverse primers (10 M), and 13.6 L of autoclaved Milli-Q water. Amplicons were sequenced on an Illumina MiSeq platform using a V3 600-cycle kit (2  300 bp paired-end reads).
library_name: Leonardo076-Fang-ITS-31_S76_L001
identifiers.namespace: SUB15371376
identifiers.id: Leonardo076-Fang-ITS-31_S76_L001