title: Fecal metagenome of Hipposideros speoris from Hyderabad
design: Genomic DNA was amplified for COI gene by ANML LCO1490 and COI-CFMRa primers using KAPA HiFi HotStart PCR Master Mix. The forward and reverse primer concentration was kept at 5uM each. Further the amplicons were analyzed on 1.2% agarose gel and purified using Jetseq clean up beads. Sequencing libraries were prepared with Illumina-compatible NEXTflex Rapid DNA sequencing Bundle at Genotypic Technology Pvt. Ltd., Bangalore, India. 50-100 ng of Qubit quantified purified amplicons were end-repaired, adenylated and ligated to Illumina multiplex barcode adaptors as per NEXTflex Rapid DNA sequencing bundle kit protocol.
library_name: SO_10845_Set3_Hyd_S
identifiers.namespace: SUB15406508
identifiers.id: SO_10845_Set3_Hyd_S