title: Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients
design: Single-cell RNA sequencing of bone marrow HSPCs isolated from patients after myocardial infarction and control patients
library_name: Ctrl_patient_6_7_p
library_construction_protocol: Human bone marrow was isolated from the sternum during surgery from patients who had experienced a myocardial infarction, as well as from control patients. The samples were subjected to Ficoll separation and subsequently frozen. On the day of sorting, the samples were thawed, and a total of 7,000 to 10,000 human hematopoietic stem and progenitor cells (HSPCs) (Lineage negative, CD38 negative, CD34 positive) were sorted in 2% BSA in PBS in two biological replicates. n/a The quality of the obtained cDNA and final libraries was assessed by capillary electrophoresis (Fragment analyzer, HS NGS Fragment Kit, Agilent).
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-13509_2:Ctrl_patient_6_7_p