title: Transcriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cells
design: Transcriptomic profiling of human primary NK cells treated with cortisol and/or co-cultured with K562 target cells
library_name: Sample 14_p
library_construction_protocol: Human PBMC-derived NK cells were cultured with or without cortisol (1 µM) and, in parallel, co-cultured with K562 target cells for 6 hours. At the end of treatment, NK cells were sorted to high purity by flow cytometry, collected directly into RNase-free tubes, and immediately processed for RNA extraction. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer's instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation. RNA-seq libraries were generated by Novogene using their standard mRNA-seq workflow. Briefly, poly-A–enriched mRNA was isolated, fragmented, and reverse-transcribed to cDNA, followed by end repair, A-tailing, adaptor ligation, and PCR amplification. Libraries were purified, quantified, and assessed for size distribution prior to sequencing.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16365:Sample 14_p