title: Amplicon of root vole: LD4
design: PCR amplification employs specific primers targeting the V3-V4 region of bacterial 16S rRNA. PCR products are quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (BioTek, FLx800), followed by pooling according to the required data volume per sample. Sequencing: The pooled library (10 nM) was diluted to an appropriate concentration and subjected to 2250 bp paired-end sequencing on an Illumina NovaSeq instrument using the NovaSeq 6000 SP Reagent Kit (503 cycles).
library_name: LD4
identifiers.namespace: SUB15671371
identifiers.id: LD4