title: DNA sequence of TrxA-C1-8 expressed from pcDNA plasmid
design: Random DNA sequences were cloned into the Thioredoxin backbone. Random peptides expressed from the TrxA backbone were selected that interacted with the yeast Apc10 protein via a yeast 2-hybrid screen. The TrxA-peptide construct was originally fused to an NLS, an activation domain and an HA motif. The HA-TrxA-peptide construct was subsequently cloned into the human pcDNA expression vector, as described here.
library_name: pcDNA-peptide C1-8
identifiers.namespace: SUB16140552
identifiers.id: pcDNA-peptide C1-8