title: GSM8695117: luc7a1 and luc7b double knockout replicate 3; Arabidopsis thaliana; RNA-Seq
library_name: GSM8695117
library_construction_protocol: Cell line: RNA was extracted 24 hours after transfection using the Qiagen RNeasy Mini kit (cat. no. 74104) according to the manufacturer's instructions with the optional on-column DNase digestion (cat. No 79254). Seedlings: Seedlings were collected and flash frozen in liquid nitrogen. Total RNA was isolated using RNeasy® Plant Mini Kit (Qiagen, cat. nos. 74904) according to the manufacturer's instructions. Cell line: Illumina-compatible libraries were prepared by MIT BioMicroCenter using NEB II Ultra Directional RNA with poly(A) selection and sequenced on NovaSeq 6000 with 2 x 150 bp reads. Seedlings: mRNA stranded library preparation and sequencing (PE150) was done by Novogene (Cambridge, United Kingdom) using an Illumina Novaseq6000 system.
identifiers.namespace: GEO
identifiers.id: GSM8695117_r1