title: GSM8471348: scRNAseq_Utrn-Mdx_Immune-delpleted_muscle_rep3; Mus musculus; RNA-Seq
library_name: GSM8471348
library_construction_protocol: Single cells were collected from mouse muscle by disection and mincing of muscle tissue, digestion using collagenase II and dispase in wash medium, and training using a 20-gauge needle and a 40 micrometer filter. For samples with immune depletion, cd45 antibody was used to remove immune cells by FACS. 16000 cells were counted using a hemocytometer and loaded onto a 10x Genomics Chromium chip per manufacturer's recommendations. Reverse transcription and library preparation was performed using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10x Gemomis, CG000204) according to manufacturer's protocols. After a cleanup with SPRIselect Reagent Kit (Beckman, C10640), final libraries were quantified using a Qubit™ 1X dsDNA HS Assay Kit (Invitrogen, Q33231) and fragment size distribution was verified using a High Sensitivity D1000 Tapestation (Agilent, 5067-5582).
identifiers.namespace: GEO
identifiers.id: GSM8471348_r1