title: RNA-seq of primary human fetal RPE cells (hfRPE) treated with IL1α, with and without prior high-dose zinc supplementation, compared to untreated controls
design: RNA-seq of primary human fetal RPE cells (hfRPE) treated with IL1α, with and without prior high-dose zinc supplementation, compared to untreated controls
library_name: 125Zn_3_p
library_construction_protocol: After removing the culture media, lysis buffer was added on top of the cultures, then lysed cells were transferred to RNase-free eppendorf tubes.  The RNA of a total of 11 samples (3 biological replicates of each age, except for 25 weeks, with 2 biological replicates) was extracted using RNeasy Mini Kit (Qiagen), according to the manufacturer's protocol mRNA enrichment and purification was performed using oligo(dT) coupled to magnetic beads. cDNA library construction involved mRNA fragmentation, cDNA synthesis (generating first-strand cDNA with random hexamer-primed reverse transcription, followed by a second-strand cDNA synthesis), end-repair, A-tailing and adapter ligation, followed by PCR amplification, circularization and rolling circle amplification for DNA nanoball (DNB) generation, which harbored more than 300 copies of one molecule. 
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-15373:125Zn_3_p