title: Root sample for 16S amplicon sequencing (ts2COM2 Root WT 5) (CRX1168393; CRA018546)
library_name: missing
library_construction_protocol: DNA for each sample were extracted by Power Soil DNA Isolation Kit (Qiagen, Germany), then the V3-V4 region of the 16S rDNA was alpliedied by two-step PCR,Library was prepared following the protocol of the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA). Finally,  the amplicons were sequenced on Illumina Novaseq 6000 platform in pair-end 250 bp mode.
identifiers.namespace: NGDC
identifiers.id: CRX1168393
identifiers.namespace: Southern University of Science and Technology
identifiers.id: DRX802649