title: Transcriptomic profiling of CAR-NK and NR3C1-knockout CAR-NK cells exposed to hydrocortisone and hypoxia-mimicking conditions
design: Transcriptomic profiling of CAR-NK and NR3C1-knockout CAR-NK cells exposed to hydrocortisone and hypoxia-mimicking conditions
library_name: Sample 3_p
library_construction_protocol: CAR-NK-92, and NR3C1-knockout CAR-NK-92 cells were cultured under defined experimental conditions. Cells were treated with hydrocortisone and/or CoCl₂ and subsequently co-cultured with A549 target cells for 6 hours. Following co-culture, NK-92 cells were collected, washed in cold PBS, and pelleted in RNase-free tubes. Cell pellets were processed immediately for RNA extraction. Total RNA was extracted from sorted NK cells using the Qiagen RNA extraction kit following the manufacturer's instructions (including on-column DNase digestion when applicable). RNA quantity and purity were assessed using NanoDrop spectrophotometry. High-quality RNA (RIN ≥ 7) was used for downstream library preparation. RNA-seq libraries were sequenced by Novogene using an Illumina platform, generating paired-end reads. Standard Illumina sequencing chemistry and quality control procedures were applied. Raw FASTQ files were delivered after base calling, filtering, and adapter trimming performed by Novogene.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16367:Sample 3_p