title: mRNA-seq of HEK293: QAL333 0uM
design: Total RNA was isolated using Trizol reagent. RNA quality was assessed using the Agilent TapeStation 4200, and quantification was performed with Qubit 4. Poly(A)+ RNA was enriched using the Lexogen Poly(A) RNA Selection Kit. Libraries were prepared with the CORALL RNA-Seq V2 Library Prep Kit (Lexogen) and indexed using Illumina indexes 112. Library quality and size distribution were evaluated with the Agilent TapeStation 4200 or 2100 Bioanalyzer, and quantified by qPCR. Sequencing was performed on the Illumina NovaSeq 6000 using paired-end 150 bp reads.
library_name: ctr_3
identifiers.namespace: SUB15406478
identifiers.id: ctr_3