title: wt-hematopoetic01
design: For single-cell RNA sequencing, bone marrow, spleen, and thymus were collected from 4 WT, 2 APOBEC2 KO (Apobec2/), and 2 Het (Apobec2/+) female C57BL/6 mice. Hematopoi- etic progenitors and stem cells were enriched from bone marrow using c-kit magnetic beads. Flow cytometry staining involved Sca-1, CD34, c-Kit, Lineage, FLT3, and IL7R antibodies for bone marrow, and CD4, CD8, CD3, Gr1, B220, and CD19 for spleen and thymus. Cells were sorted into StemSpan medium, then CD45+ cells, LS-K, LSK, lymphoid progenitors, and mature cells were sequentially sorted. After centrifugation, cells were prepared for scRNA-seq using the 10x Genomics Chromium platform. Samples were pooled from two mice per group before sequencing.
library_name: dbbdc966709d2dd762ac1e1a409dad80
identifiers.namespace: SUB14873109
identifiers.id: dbbdc966709d2dd762ac1e1a409dad80