title: GSM8636782: Input; Homo sapiens; ChIP-Seq
library_name: GSM8636782
library_construction_protocol: The cells were harvested, lysed and ysates were immunoprecipitation with acK130-H2a antibodies (2 ug) for 3 hours. The magnetic beads were used to capture immunoprecipitates, washed and bound chromatin was eluted, followed by protease treatment. Ten nanograms of immunoprecipitated DNA was fragmented to 300 base pairs using a Covaris M220 Focused-ultrasonicator (Covaris, Inc., Woburn, MA) and then used to generate sequencing libraries using the Kapa Hyper Prep Kit (Roche Sequencing Solutions Inc., Pleasanton, CA).
identifiers.namespace: GEO
identifiers.id: GSM8636782_r1