title: RNA-seq_Brycon_amazonicus
design: Matrinxa juveniles were obtained from a commercial farm in Itacoatiara, Amaznas, Brazil (-3.100651, -58.862324) and transported to Universidade Nilton Lins, Manaus, Brazil, where they were anaesthetized. Fresh samples from liver, kidney and gill were quickly collected and placed in an RNA stabilization buffer (RNAlater, Invitrogen, USA), stored at 4C for 24h and subsequently placed at -80C for long-term preservation. Total RNA extraction from the three tissues was performed using the Maxwell RSC simply RNA Tissue Kit (Promega, Madison, WI, USA), following the manufacturer's instructions on the Maxwell RSC 16 instrument (Promega Corporation, Madison, WI, USA). The resulting high-quality total RNA was sent to Novogen, Inc. (Germany) for strand-specific libraries (insert size of 250300 bp) construction and total RNA sequenced of 150 bp paired-end reads on the Illumina NovaSeq 6000 platform.
library_name: Baliver
identifiers.namespace: SUB15371349
identifiers.id: Baliver