title: GSM9661745: sham5; Mus musculus; RNA-Seq
library_name: GSM9661745
library_construction_protocol: Total RNA was isolated from mouse peripheral blood using TRIzol reagent, followed by DNase I treatment to remove genomic DNA contamination. RNA integrity was assessed using an Agilent 2100 Bioanalyzer, and samples with RIN value ≥ 7 were used for subsequent experiments. mRNA was enriched from total RNA using poly-T magnetic beads. The enriched mRNA was fragmented, reverse-transcribed into cDNA, ligated with sequencing adapters, and amplified by PCR to construct the sequencing library.
identifiers.namespace: GEO
identifiers.id: GSM9661745_r1