title: GSM9324319: Interferon alpha, rep4; Homo sapiens; RNA-Seq
library_name: GSM9324319
library_construction_protocol: RNA was extracted using a standard Trizol extraction protocol. RNA libraries were made using the Illumina TruSeq Stranded mRNA kit which utilizes oligo-dT beads to select and amplify mRNA. cDNA was synthesized using nine rounds of PCR and quality and concentrations of the DNA libraries were assayed using the Agilent 2100 Bioanalyzer high sensitivity DNA assay and the DNA high sensitivity Qubit. Raw bulk RNA-seq data was initially assessed for quality using FastQC 0.11.9, and results summarized with MultiQC 1.10.1. Data then underwent quality trimming and adapter removal using Trimmomatic 0.39.
identifiers.namespace: GEO
identifiers.id: GSM9324319_r1