title: Illumina NovaSeq 6000 paired-end Sequencing of KAS20002692 (KAE21035530)
design: missing
library_name: Agxt2_KD_ND_1_TRANSCRIPTOMIC
library_construction_protocol: Total RNAs were prepared from liver tissues of Agxt2-knockdown and control mice. The libraries were prepared for 150bp paired-end sequencing using TruSeq Stranded Total RNA Sample Prep Kit with Ribo-Zero H/M/R Illumina, CA, USA. Namely, mRNA molecules were purified and fragmented from 1microgram of total RNA using oligo dT magnetic beads. The fragmented mRNAs were synthesized as single-stranded cDNAs through random hexamer priming. By applying this as a template for second strand synthesis, double-stranded cDNA was prepared. After sequential process of end repair, A-tailing and adapter ligation, cDNA libraries were amplified with PCR Polymerase Chain Reaction. Quality of these cDNA libraries was evaluated with the Agilent 2100 BioAnalyzer Agilent, CA, USA. They were quantified with the KAPA library quantification kit Kapa Biosystems, MA, USA according to the manufacturer's library quantification protocol. Following cluster amplification of denatured templates, sequencing was progressed as paired-end 2x150bp using Illumina Novaseq6000 Illumina, CA, USA.
identifiers.namespace: KOBIC
identifiers.id: KAE21035530
identifiers.namespace: Seoul National University College of Medicine
identifiers.id: DRX602127