title: RNA-seq of Zizania palustris leaf replicate 1 (CRX1854792; CRA027852)
library_name: missing
library_construction_protocol: Total RNA was extracted from each sample and assessed for purity, concentration, and integrity. Poly(A)+ mRNA was enriched using oligo(dT) magnetic beads and fragmented. First-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. Double-stranded cDNA was end-repaired, A-tailed, and ligated with sequencing adapters. After size selection and PCR enrichment, the libraries were sequenced with a paired-end 150 bp strategy.
identifiers.namespace: NGDC
identifiers.id: CRX1854792
identifiers.namespace: Shanghai Normal University
identifiers.id: DRX797537