title: Profiling of hot spring microbiomes using V3V4-region of 16S rRNA gene
design: DNA was isolated from wet sediments using FastDNA Spin Kit for Soil (MP Biomedicals, USA) including mechanical cell lysis using a FastPrep-24 5G homogenizer (MP Biomedicals, USA). Amplicon libraries were prepared using primers for the V3V4 region of 16S rRNA gene. PCR was performed on a StepOne Plus Real-Time instrument (Thermo Fisher Scientific, USA).
library_name: 4704_repl1
identifiers.namespace: SUB15669720
identifiers.id: 4704_repl1