title: GSM8636843: RNAEV; Homo sapiens; RNA-Seq
library_name: GSM8636843
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen) following the manufacturer's instructions. In the processing of Ribo-seq, to block translational elongation, 100 µg/ml of cycloheximide was added to the cell culture medium, and the cell extracts were collected after treatment. Subsequently, ribosome-protected fragments (RFs) were generated by adding RNase I and DNase I, and rRNA was removed using an RNA purification kit and antisense DNA probes, ultimately constructing the Ribo-seq library. Ribo-seq library was created using the NEBNext® Small RNA Library Prep Set for Illumina, RNA-seq cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina
identifiers.namespace: GEO
identifiers.id: GSM8636843_r1