title: GSM9033658: k_283; Mus musculus; RNA-Seq
library_name: GSM9033658
library_construction_protocol: Lung and kidney tissues were enzymatically digested into single-cell suspensions, and endothelial cells were enriched using anti-CD31 magnetic-microbeads according to the manufacturer's protocol. CD31⁺ cells were lysed in Trizol reagent (Thermo Fisher Scientific), and total RNA was purified using RNeasy Micro columns (Qiagen) with on-column DNase digestion to eliminate genomic DNA contamination. RNA-seq libraries were generated using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), including polyA enrichment for mRNA. Libraries were barcoded, quantified by qPCR, and sequenced on an Illumina NovaSeq 6000 using 150 bp paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM9033658_r1