title: GSM8637907: smRNAseq_Flower_b2_WT_rep3; Arabidopsis thaliana; ncRNA-Seq
library_name: GSM8637907
library_construction_protocol: Total RNA extraction and smRNA enrichment was performed following a previously reported protocol. Briefly, 10 μg of total RNA extracted from flower buds (stage 12 and younger) were used for each smRNA library prep. The smRNA-seq libraries were constructed using the NEBNext Small RNA Library Prep Kit (NEB, Cat# E7330L). The smRNA libraries were further purified using an 8% acrylamide gel and size selected (130-160 bp) relative to the O'RangeRuler 10 bp DNA Ladder (Thermo Scientific, Cat# SM1313). The size selected smRNA libraries were pooled for sequencing with single-end 50 bp kit (SE50) on Illumina Nextseq 2000 or Novaseq X plus platforms.
identifiers.namespace: GEO
identifiers.id: GSM8637907_r1