title: GSM9501088: CITE_Colons3; Mus musculus; OTHER
library_name: GSM9501088
library_construction_protocol: Immune cells from the SB and colon LP and EP were isolated using the Miltenyi lamina propria dissociation kit (130-097-410) using the manufacturer's instructions with the following changes: Initial shaking incubations in predigestion solution and RPMI were done for 10 min and repeated until the LP tissue turned pink. Both LP and EP were then suspended in 5 mL of 40% percoll each and underlaid with 2.5 mL of 80% percoll. The interface was harvested and washed twice in PBS. CD45+ immune cells were enriched with a bead-based positive selection kit (StemCell, 18945). KO cells were incubated with TotalSeq-C0301 hashtag (Biolegend, 155861), het cells were incubated with TotalSeq-C0302 hashtag (Biolegend, 155863), and WT cells were incubated with TotalSeq-C-0303 hashtag (Biolegend, 155865). Hashtagging was done for 30min on ice with all hashtagging antibodies at 1:200. Samples were then combined by organ for scRNA sequencing analysis. Library preperation was done as according to the manufacterers instructions (Illumina Next GEM 5' v2)
identifiers.namespace: GEO
identifiers.id: GSM9501088_r1