title: 16SRNA of gut microbiota: melanoma patient
design: Fecal samples were collected from patients with BRAF V600-mutated metastatic melanoma undergoing first-line treatment with BRAF/MEK inhibitors. Microbial DNA was extracted and the hypervariable regions V2-4-8 and V3-6,7-9 of the bacterial 16S rRNA gene were amplified using the Ion16S Metagenomics Kit. Libraries were sequenced on the Ion Torrent S5 platform. The aim was to evaluate gut microbiota composition at baseline and during treatment, and to associate bacterial profiles with treatment response and toxicity.
library_name: LIB001
identifiers.namespace: SUB15369283
identifiers.id: LIB001