title: S24-4 (CRX1819289; CRA027275)
library_name: missing
library_construction_protocol: Total microbial genomic DNA was extracted from midguts using TIANamp Genomic DNA Kit (TIANGEN Biotech, Cat# DP304-02). The hypervariable region V3-V4 of the bacterial 16S rRNA gene were amplified with primer pairs 338F and 806R by an T100 thermal cycle (BIO-RAD, USA). The PCR product was extracted from 2% agarose gel and purified. Purified amplicons were pooled in equimolar amounts and paired- end sequenced on an illumine NextSeq 2000 PE300 platform according to the standard protocols by Majorbio Bio-Pharm Technology Co. Ltd (Shanghai, China). submitted_instrument_model: Illumina NextSeq 2000
identifiers.namespace: Huazhong University of Science and Techn
identifiers.id: DRX794858
identifiers.namespace: NGDC
identifiers.id: CRX1819289