title: trnL metabarcoding of Polyphylla laticollis larval gut content replicate 1
design: Plant chloroplast trnL (UAA) intron P6 loop amplified using universal primers trnL_g (5-GGGCAATCCTGAGCCAA-3) and trnL_h (5-CCATTGAGTCTCTGCACCTATC-3) (Taberlet et al., 2016). Total genomic DNA extracted from larval gut contents using E.Z.N.A. Soil DNA Kit (Omega Bio-tek). PCR performed with TransStart Fastpfu DNA Polymerase. Sequencing on Illumina NovaSeq (Biozeron, Shanghai).
library_name: YH1
identifiers.namespace: SUB16144085
identifiers.id: YH1