title: =_xlfn.CONCAT("WGS of Danio Rerio ",B11)
design: Larvae were pooled and flash frozen on dry ice then stored at -20C. Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (QIAGEN, 69504) according to the included protocol for Tissue. To achieve sufficient lysis, reagents were scaled 1.5X for pre-wash steps and the proteinase K digest was 15 minutes. DNA was eluted in nuclease-free water. DNA was prepared for sequencing using the Illumina DNA Prep, Tagmentation kit (Cat#20018705) and Nextera DNA CD Indexes (Cat#20018707) using an input of 200 ng and 5 PCR cycles per the user manual.  Sequencing was performed on an Illumina NextSeq500 as a 150 bp single-end run with dual (8x8 bp indexing). ~30X coverage (~268 million reads) was acquired for each sample.
library_name: olympic_sibling
identifiers.namespace: SUB14861326
identifiers.id: olympic_sibling