title: RNA-Seq of Tasmannia lanceolata: Seed from medium berry
design: RNA was extracted using the QIAGEN RNeasy Plant Kit. RNA sequencing libraries were synthesised for each sample using NEBNext Ultra II stranded RNA library synthesis kits, indexed using the NEBNext Multiplex Oligos for Illumina barcode kit. Libraries were quantified using NEBNext Library Quant KIt for Illumina, normalised, pooled, and sequenced on an Illumina HiSeq X Ten platform then demultiplexed and error-corrected using Rcorrector. Adapters and low quality base pairs were trimmed using TrimGalore (v0.6.0). Ribosomal RNA sequences were discarded when one of the paired-end reads mapped to the sequences present in the SILVA database using Bowtie2.
library_name: MS_final_R1
identifiers.namespace: SUB14860230
identifiers.id: MS_final_R1