title: 16S Metagenomics: bacterial species identification from Vitis vinifera samples
design: The NGS sequencing project started with quality control of the DNA samples, where the DNA was analysed to ensure samples had sufficient integrity (1,5% Agarose gel electrophoresis) and quantity (Qubit) for optimal amplification;  The library construction was performed using the Illumina 16S Metagenomic Sequencing Library preparation protocol with a specific set of primers: 341F (CCTACGGGNGGCWGCAG) and 785R (GACTACHVGGGTATCTAATCC);  The generated DNA fragments (DNA libraries) were sequenced with MiSeq Reagent Kit v3 in the lllumina MiSeq platform, using 300bp paired-end sequencing reads.
library_name: N2924-16S_Lib
identifiers.namespace: SUB14896648
identifiers.id: N2924-16S_Lib