title: GSM9115020: HeLa_TurboID-only_rep2 PLAMseq; Homo sapiens; OTHER
library_name: GSM9115020
library_construction_protocol: Cells were fixed with 1% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was sonicated using a E220 focused-ultrasonicator (Covaris). PLAMseq libraries of H1.4 and H1.0 samples were prepared using Tn5 transposase (tagmentation) 5 minutes 37ºC. Then, NEBNext® High-Fidelity 2X PCR Master Mix was used for PCR amplification and library indexing (Nextera DNA Indexes i5 and i7) according to the following program: 72°C for 5 minutes; 98°C for 30 seconds, 9-10 cycles of 98°C for 10 seconds, 63°C for 30 seconds, and 72°C for 30 seconds, final holding stage 72ºC 5 minutes. PCR product was double purified using 0.7 and 0.15 volumnes of Seramag Beads (Cytiva). PLAMseq libraries of CTCF, POL2RI and H1.2 samples were prepared using the ThruPLEX DNA-Seq kit of Takara (cat. N. R400675).
identifiers.namespace: GEO
identifiers.id: GSM9115020_r1