title: RNA-seq of 5dpf zebrafish tert F2 larvae with/without injury
design: RNA-seq of 5dpf zebrafish tert F2 larvae with/without injury
library_name: Sample 9_p
library_construction_protocol: All RNA analyses were performed using triplicates for all conditions using material isolated from the muscle of 30 larvae using Tri-reagent. Total RNA was isolated from dissected injured trunk regions of larvae at 24h post injury (hpi). Tissue was homogenised in TriReagent (Sigma) and total RNA isolated according to the manufacturerâ€™s protocol. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. 
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-15577:Sample 9_p