title: Whole exome sequencing of Homo sapiens: Breast cancer metastatic tumor, Brain frozen tissue
design: Extraction of DNA from the isolated frozen and FFPE breast cancer tumor tissue was performed using the Gentra Puregene Tissue Kit (QIAGEN, CAT # 158066) according to the manufacturer's protocol. Extracted DNA was quantified using Qubit (Thermo Fisher Scientific).  DNA-seq libraries were prepared for each sample using the Kapa Hyper Prep kit (Roche, Cat # KK8504) and indexed using IDT for Illumina DNA unique dual indexes (UDI) Set A. Final libraries were quality checked using 2100 Bioanalyzer (Agilent Technologies) and Qubit 2.0 (ThermoFisher Scientific) and pooled in batches of 12 (pre-capture pooling).  Each pool of libraries were then hybridized with IDT xGen Exome Hybridization Panel V2 probes (IDT, CAT # 10005152) to capture and pull down the portion of the DNA-seq library representing the human the exome. Final captured libraries were amplified, pooled into 1 library, and sequenced using a total of 6 lanes of an Illumina NovaSeq 6000 S4 flow cell (batch 1 with 2 lanes and batch 2 with 4 lanes), generating 150bp PE reads. Sequence data was demultiplexed and Fastq files generated using Illumina Bcl2Fastq conversion software.
library_name: DCBSM-NC010-10117-M07-02A-01D_dnaA6_L004
identifiers.namespace: NCI-phs003673
identifiers.id: DCBSM-NC010-10117-M07-02A-01D_dnaA6_L004