title: GSM8472313: Wild type, female, 4; Mus musculus; RNA-Seq
library_name: GSM8472313
library_construction_protocol: Mice were anesthetized (Isoflurane, Pivetal, Liberty, MO), decapitated, and the ventral striatum was collected by microdissection. Samples were snap-frozen in liquid nitrogen.and stored at -80 degrees. Total RNA was extracted from the ventral striatum from each animal using  a RNeasy mini kit (Qiagen, 74104) according to manufacturer's directions. A cDNA library was prepared for each sample using the Illumina TruSeq stranded mRNA library kit. Libraries were barcoded for multiplexing with IDT for Illumina 8 nucleotide i7 and i5 unique dual indexes. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Bioanalyzer high sensitivity DNA chip (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq 6000 located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data.
identifiers.namespace: GEO
identifiers.id: GSM8472313_r1