title: GSM8465716: CX2203; Homo sapiens; RNA-Seq
library_name: GSM8465716
library_construction_protocol: RNA extraction was performed using illustra RNAspin Mini Kit (GE Healthcare Life Sciences), following manufacturer's instructions. RNA concentration and quality were measured using SimpliNano (GE Healthcare Life Sciences). cDNA was synthesised by reverse transcription of RNA. RT-qPCR was performed using TaqManTM Fast Advanced Master Mix (4444554; Thermo Fisher Scientific, Lithuania) and Taqman gene expression assay probe for ACTA2 (Hs00426835_g1) and the housekeeping gene 18s rRNA control mix  (4318839; Applied Biosystems, Kingsland Grange, Woolston, Warrington, UK). RT-qPCR was performed on QuantStudio™ 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Scoresby, VIC, Australia), following manufacturer's instructions. RNA was extracted using the Illustra RNAspin Mini Kit (GE Healthcare Life Sciences) according to manufacturer's instructions. RNA quality was checked by bioanalyzer, followed by library construction using the TruSeq Stranded mRNA kit (Illumina) and sequenced using Novaseq 6000 (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM8465716_r1