title: RNA virome of seawater with suspected or verified K. brevis blooms
design: From each sample, cDNA was synthesized from 8 L RNA and 50 ng random hexamers using the SuperScript IV First-Strand Synthesis System (Invitrogen). Second strand synthesis was performed on each cDNA product using New England Biolabs DNA Polymerase I, Large (Klenow) Fragment. Double-stranded cDNA samples were purified with DNA Clean & Concentrator-25 kit (Zymo Research) and fragmented to 300 bp using the Covaris M220 Focused-ultrasonicator. NGS libraries were prepared from fragmented cDNA samples using the xGen ssDNA & Low-Input DNA Library Preparation Kit (Integrated DNA Technologies) following the manufacturers protocol for DNA concentrations <1 ng/L. All libraries were sequenced by Azenta Life Sciences on the 2x150 bp Illumina HiSeq platform.
library_name: Pool2_15
identifiers.namespace: SUB14670191
identifiers.id: Pool2_15