title: 16S rRNA-seq microbiome of soil: soil with added root exudates
design: DNA was extracted from 0.25 g of fresh (frozen) soil using the DNeasy PowerSoil Pro Kit (QIAGEN N.V., Germany) according to the manufacturers instructions. The V4 region of the bacterial 16S rRNA gene was amplified using the primer pair CS1_515FB and CS2_806RB (Apprill et al., 2015; Parada et al., 2016). Sequencing library preparation included two PCR steps. The first-round PCR (20 L total volume) contained 1 L of normalized extracted DNA, 0.2 L of DreamTaq DNA Polymerase (5 U/L), 2 L of 10 DreamTaq Buffer, 2 L of 1 mM dNTPs, 0.6 L of each primer (10 M), and 13.6 L of autoclaved Milli-Q water. Amplicons were sequenced using the Illumina MiniSeq platform with a 2  150 bp paired-end run.
library_name: Hinojosa064-Fang-16S-30_S64_L001
identifiers.namespace: SUB15371376
identifiers.id: Hinojosa064-Fang-16S-30_S64_L001