title: RNA-Seq of Procambarus clarkii:adult abdominal ganglion
design: The normal and thermotolerant crayfish (Procambarus clarkii) originated from a farm in Wuhu, China, and had been maintained under standard farming conditions for at least three generations. Before temperature stress, all experimental animals were acclimated for 48h at 250.5C, >6.0mg/L dissolved oxygen, pH7.27.6, and a 12L:12D photoperiod. A total of 300 healthy, uniform-sized individuals not in the molting stage were randomly selected. Temperature stress was applied by gradual change (1C per 4h) from 25C to either 8C or 35C, with sustained exposure for 12h or 24h, respectively. Six treatment groups were used with three biological replicates per group; each replicate consisted of pooled abdominal ganglion tissue from five crayfish. After treatment, crayfish were anesthetized on ice, and abdominal ganglion were dissected, preserved in RNAlater and then stored at 80C.
library_name: NP_control_1
identifiers.namespace: SUB16072846
identifiers.id: NP_control_1