title: RNA-Seq of lactating Bos Taurus rumen fluid
design: DNA was extracted from 250 mg of rumen fluid using the repeated bead beating and column purification method as described by Yu and Morrison (2004). DNA quality was assessed on agarose gels and DNA concentration was quantified using a Nanodrop 1000 spectrophotometer. The DNA samples were then standardised to a volume of 25 l at a concentration of 20 ng/l of DNA per sample. Amplicons were generated targeting the V4 region of the 16S rRNA gene using the 515F/806R primers on Illumina technology. Sequencing libraries were generated, and indexes were added. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount required.
library_name: R1.9360
identifiers.namespace: SUB15369667
identifiers.id: R1.9360