title: MeRIP sequencing analyses of human liver cancer cell line HepG2
design: MeRIP sequencing analyses of human liver cancer cell line HepG2
library_name: HepG2-shCtrl-Input_p
library_construction_protocol: HepG2 cells were cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum. The cells were maintained at 37 °C in a 5% CO2 humidified atmosphere. When the cell density reached approximately 80%, the cells were collected. 1x10^7 HepG2 cells were used in shCtrl and YTHDC1 knockdown group. Total RNA was isolated by Trizol (Invitrogen) according to the manufacturer's protocols. RNA was purified by NEBNext® rRNA Depletion Kit (NEB, USA). Fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The RNA libraries were constructed using the NEBNext Ultra Directional RNA Library PrepKit (NEB, USA).
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14194:HepG2-shCtrl-Input_p