title: Xbp1-3 (CRX1512686; CRA022830)
library_name: missing
library_construction_protocol: Total RNA was extracted using Trizol according to the manufacturers instructions. The quality and quantity of the RNA were assessed using the Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit.From 5 ug of total RNA, mRNA was isolated using Dynabeads Oligo-dT through 2 rounds of purification. The purified mRNA was fragmented and then reverse-transcribed into cDNA using SuperScript II Reverse Transcriptase. The first-strand cDNA was then used to synthesize U-labeled DNA.The U-labeled Heat-labile UDG enzyme was used to remove the U-labeled regions. The resulting fragments were amplified by PCR.
identifiers.namespace: Air Force Medical University
identifiers.id: DRX804973
identifiers.namespace: NGDC
identifiers.id: CRX1512686