title: RWU2 fungal ITS1 amplicon sequencing
design: Fungal community profiles were generated by ITS1 amplicon sequencing. DNA was extracted from calcareous soil microcosms and quality checked by 1% agarose gel electrophoresis and NanoDrop One. PCR amplification used barcode indexed ITS1 primers and Premix Taq (TaKaRa). Amplicons were quantified, pooled in equimolar amounts, gel purified (E.Z.N.A. Gel Extraction Kit), and eluted in TE buffer. Libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina and sequenced on an Illumina platform. Raw reads are provided as paired end FASTQ files.
library_name: RWU2
identifiers.namespace: SUB15966430
identifiers.id: RWU2