title: PacBio HiFi sequencing data from Tamalia coweni: DMB7
design: The HiFi SMRTbell library was constructed using the SMRTbell gDNA Sample Amplification Kit (Pacific Biosciences, Menlo Park, CA; Cat. #101-980-000) and the SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences; Cat. #100-938-900) according to the manufacturer's instructions. Approximately 10 kb sheared DNA by the Megaruptor 3 system (Diagenode, Belgium; Cat. #B06010003) was used for removal of single-strand overhangs at 37C for 15 minutes, DNA damage repair at 37C for 30 minutes, end repair and A-tailing at 20C for 30 minutes and 65C for 30 minutes, and ligation of overhang adapters at 20C for 60 minutes. To prepare for library amplification by PCR, the library was purified with ProNex beads (Promega, Madison, WI; Cat. # NG2002) for two PCR amplification conditions at 15 cycles each then another ProNex beads purification.  Purified amplified DNA from both reactions were pooled in equal mass quantities for another round of enzymatic steps that included DNA repair, end repair/A-tailing, overhang adapter ligation, and purification with ProNex Beads. The PippinHT system (Sage Science, Beverly, MA; Cat # HPE7510) was used for SMRTbell library size selection to remove fragments < 6-10 kb. The 10  11 kb average HiFi SMRTbell library was sequenced at UC Davis DNA Technologies Core (Davis, CA) using one 8M SMRT cell, Sequel II sequencing chemistry 2.0, and 30-hour movies each on a PacBio Sequel II sequencer.
library_name: CCGP_gDNA0085
identifiers.namespace: SUB15342392
identifiers.id: CCGP_gDNA0085