title: GSM8472170: HEK293T empty shRNA control vector PAS-seq Replicate 3; Homo sapiens; OTHER
library_name: GSM8472170
library_construction_protocol: Total RNA was extracted from Trizol following the manufacturer's protocol PAS-seq libraries were prepared as previously described (PMID: 34183125),  with the following minimal modifications. Purified mRNA was fragmented at 94˚C for 3 minutes using the NEBNext Magnesium RNA fragmentation module. In addition, the final libraries were run out on a 2.5% standard agarose gel and the region between 200-300 basepairs was gel extracted and sequenced.
identifiers.namespace: GEO
identifiers.id: GSM8472170_r1