title: RNA-Seq of bos taurus: oocyte
design: we transferred the RNA-containing cytoplasm into 4.5 L reverse transcription mix containing 100 U SuperScript II Reverse Transcriptase (18064071, Invitrogen), 3 M 6-base pair (bp) barcode primer, 10 M template switch oligo primer, 4 U RNase inhibitor, 1 mM deoxynucleotide triphosphates (4030, Takara Bio Inc.), and 60 mM MgCl2. Then, the mixture underwent polymerase chain reaction (PCR) at 25C for 5 min, 42C for 60 min, 50C for 30 min, and 70C for 10 min in a thermocycler. Then the purified cDNA was subjected to biotin PCR and enriched. Next, the KAPA HyperPrep Kit (Illumina platforms) (KK8504, KAPA Biosystems Inc.) was used to construct the RNA libraries that were sequenced with 166-bp pair-end reads on an Illumina HiSeq X Ten system (Illumina Inc.)
library_name: SE_bar2_17
identifiers.namespace: SUB14672222
identifiers.id: SE_bar2_17