title: GSM8948067: HCT116 dTAG SNIP1_DMSO_RNA_seq_rep1; Homo sapiens; RNA-Seq
library_name: GSM8948067
library_construction_protocol: Cells were washed twice with ice-cold PBS, scraped, pelleted at 500g for 3 min and treated for 5 min with 500 µl of cytoplasmic lysis buffer (10 mM Tris-Cl, pH=8, 0.32 M sucrose, 3 mM CaCl2, 2 mM MgCl2, 0.1 mM EDTA, 0.5 % TritonX-100, supplemented with 40 U/ml RNase inhibitor). Cytoplasmic RNA present in the supernatant was removed by centrifugation (500g for 3 min). Nuclear RNA was isolated from the remaining nuclear pellet using Tri-Reagent (Sigma, 93289). For each sample, 250 ng of DNase treated RNA (Sigma, AMPD1) was used for rRNA depletion using RiboCop rRNA Depletion Kit (Lexogen, #144). Sequencing libraries were prepared using the NEBNext UltraExpress II Directional RNA Library Prep Kit for Illumina (NEB, E3330) according to the manufacturer protocol.
identifiers.namespace: GEO
identifiers.id: GSM8948067_r1