title: GSM8729115: ∆vsrA (JC2540) replicate A (M2G); Caulobacter vibrioides NA1000; RNA-Seq
library_name: GSM8729115
library_construction_protocol: RNA samples were prepared from 10 ml bacterial cultures. Cells were harvested at 4°C by centrifugation at 8,000 rpm for 10 minutes and immediately frozen in liquid nitrogen. The samples were stored at -80°C until their processing. RNA were extracted using the RNeasy mini kit from Qiagen following the manufacturer's recommendation. Any remaining DNA was removed by on-column DNA digestion with DNase I (RNase-free DNases set from Qiagen) followed by a TURBO DNA-freeTM DNase treatment (Invitrogen). RNA samples were then re-purified using a RNA clean-up procedure (RNeasy mini kit). RNA-seq libraries were prepared from 100 ng of total RNA with the Illumina Stranded mRNA Prep reagents (Illumina) using a unique dual indexing strategy, and following the official protocol automated on a Sciclone liquid handling robot (PerkinElmer). The polyA selection step was replaced by an rRNA depletion step with the RiboCop for Bacteria, mixed bacterial samples reagents (Lexogen).
identifiers.namespace: GEO
identifiers.id: GSM8729115_r1