title: GSM8465747: Sequencing data for 5hmC from nomal mouse cerebellum tissue by DDD-seq; Mus musculus; OTHER
library_name: GSM8465747
library_construction_protocol: Genomic DNA of mouse cerebellum tissues was extracted using Tissue DNA kit (Omega Bio-Tek Inc., Norcross, GA, USA) according to the manufacturer's recommended procedure. The DNA mixture was glycosylated and subsequently deaminated by SsdAcat. The deaminated DNA was amplificated by PCR with 5 cycles using pre-P5 primer, pre-P7 primer and Q5U Hot Start High-Fidelity DNA polymerase (New England Biolabs). After purification using 0.8× KAPA Pure beads, DNA products were then amplificated by PCR with 10 cycles using P5-index primer, P7-index primer and Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs). The PCR products were purified with 0.8× KAPA Pure beads and examined using 1.5% agarose gel electrophoresis. Library quality was assessed on the Agilent Bioanalyzer 2100 system. The library was then sequenced on an Illumina NovaSeq 6000 platform (Novogene Co., Ltd, Nanjing, China).
identifiers.namespace: GEO
identifiers.id: GSM8465747_r1