title: RNA-Seq of Aureobasidium strain 64
design: Strain was grown on the foam at the 3 ERH levels and sampled for genetic sequencing. RNA was extracted using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany). Foam samples were cut, cleaned, embedded with nutrients, and inoculated as described for the weight loss trials. The inoculated samples were incubated for 2 weeks at 50, 85, and 100% ERH. After incubation, the foam samples were cut into four pieces each and washed in 3mL of Buffer RLT from the Zymo Quick DNA Fungal Kit. This was done to remove fungal growth from the foam into a solution. The wash liquid was placed into 2mL tubes of 0.1 mm glass beads. The tubes with samples for RNA extraction were bead beaten for 5 minutes in a cold block and centrifuged. The supernatant in the tubes was then extracted using the Qiagen RNeasy Mini Kit (Qiagen, Hilden, Germany) procedure. The resulting RNA extracts each totaled 2-5 g. The RNA extracts were quality tested using a bioanalyzer before being sequenced. Extracted samples were sequenced using an Illumina NovaSeq (Illumina, CA, USA) at the University of Kansas Medical Center. The stranded mRNA library was prepared using the Universal Plus mRNA-seq with NuQuant + UID library prep (Tecan Genomics 0520-A01). The mRNA libraries were pooled for sequencing using the NovaSeq 6000 S1 Reagent Kit v1 .5-200 cycle (Illumina 20028318).
library_name: Aureo_64_RNA
identifiers.namespace: SUB14870113
identifiers.id: Aureo_64_RNA