title: RNA-Seq of mature Cathaya argyrophylla leaf
design: mRNA was enriched using oligo(dT) magnetic beads. Fragmentation buffer was added to the enriched mRNA to generate short mRNA fragments. Using these fragmented mRNAs as templates, first-strand cDNA and second-strand cDNA were synthesized following established procedures. Subsequently, the cDNA underwent end repair, A-tailing, adapter ligation, purification, PCR amplification, and product cyclization to complete the library construction. Following library quality assessment, sequencing was performed on the BGI high-throughput sequencing platform DNBSEQ-T7. Raw image data files obtained from high-throughput sequencing were converted into raw sequence reads through base calling. These raw reads were stored in FASTQ (fq) format. To ensure the reliability of downstream analyses, raw sequencing data, which may contain low-quality sequences and adapter sequences, were subjected to filtering to obtain clean reads. fastp (version: 0.21.0; parameters: default)17 was used to filter the raw fastq data, and fastqc (version: 0.11.9; parameters: default)18 was employed for quality control of the filtered data.
library_name: 10
identifiers.namespace: SUB15184596
identifiers.id: 10