title: GSM9033148: patient 210701 untreated; Homo sapiens; RNA-Seq
library_name: GSM9033148
library_construction_protocol: For cell sorting, the fresh NSCLC samples were cut into small pieces with scalpels 28 in a Petri dish and enzymatic digested at 37°C for 1h by 1mg/mL Collagenase A (Roche) and 29 0.1mg/mL DNAse I (Roche). Samples were filtered using 70µm cell strainer and washed in 30 RPMI (Gibco). After staining, cells were resuspended in PBS 0.5% BSA with 0.1mg/mL DNase for cell sorting. 10,000 Lymphocytes B cells (defined as CD45+ CD19+), 45,000 Lymphocytes T (defined as CD45+ CD3+), and 45,000 myeloid cells (defined as CD45+ HLA-DR+ CD64+) were sorted in FBS for each NSCLC sample. After centrifugation, cells were resuspended in PBS 0.5% BSA. Samples were processed for single-cell encapsulation and cDNA library generation according to the manufacturer's instructions using the 10x Genomics Single Cell 3′v2 Reagent Kit.
identifiers.namespace: GEO
identifiers.id: GSM9033148_r1