title: 16S of LEO soil microbial community: S1WB_16S
design: DNA was extracted from approximately 250 mg of homogenized soil using the FastDNA Spin Kit for Soil (MP Biomedicals, USA) following the protocol. DNA quantity and quality were assessed using a Qubit dsDNA High Sensitivity Assay Kit (Thermo Fisher Scientific, USA). The V4 hypervariable region of the bacterial and archaeal 16S rRNA gene was amplified using the primer pair 515F (5'-GTGCCAGCMGCCGCGGTAA-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Amplicon libraries were prepared following Illumina library preparation protocols with minor modifications and sequenced on an Illumina MiSeq platform using paired-end 2  150 bp chemistry.
library_name: S1WB_16S_paired_illumina
identifiers.namespace: SUB16050555
identifiers.id: S1WB_16S_paired_illumina