title: Mus musculus Testis 17dpp single-cell ATAC-seq
design: To tagment DNA, 50,000 nuclei were mixed with a modified nuclei buffer and reagents, incubated at 37C for 30 minutes, and then loaded into a BD Rhapsody Cartridge after adding sample buffer. Nuclei were sedimented, washed, and incubated with splint beads before being agitated and washed again. After lysis with DTT and proteinase K, tagmented DNA and mRNA were immobilized onto beads, followed by DNA ligation and reverse transcription using a mix containing dNTPs, RT buffer, and reverse transcriptase. Unused oligos were removed via exonuclease treatment, and DNA was eluted twice for ATAC-seq library preparation. The ATAC-seq libraries were then amplified and indexed using PCR.
library_name: 17dpp_scATAC_lib1
identifiers.namespace: SUB15191036
identifiers.id: 17dpp_scATAC_lib1