title: Targeted Amplicon NGS-sequencing of Mus musculus : N2a cells  transfected with Tpc1-I486 sgRNA and ABE8e-SpRY plasmid, replicate 1 , sequenced at the Tpc1-I486 locus , at stage E14
design: Samples were prepared for Amplicon-EZ-based NGS analysis (GeneWiz/Azenta Life Sciences). All locus-specific primers contained the 5 partial Illumina adapter sequences (Fwd: 5-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3, Rev: 5-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3). Typically, samples corresponding to different target loci from each biological replicate were pooled into a single sample. The pooled NGS data were demultiplexed by mapping to the respective reference sequences later. Here, demultiplexed samples are provided via CRISPResso2 in CRISPRessoPooled mode.
library_name: NGS_AC89
identifiers.namespace: SUB15465253
identifiers.id: NGS_AC89