title: MCF7 tetoff cells transfected with 611-CTF HER2 treated with high DMSO on 020624 replicate
design: PlateATAC-seq was performed by tagmenting ~300 nuclei per 15 L microtiter plate microwell using Tn5 transposase (1:15) in 1 tagmentation buffer supplemented with 0.1% Tween-20 and 0.01% digitonin. Nuclei were incubated with tagmentation mix for 30 min at 37 C. The reaction was quenched in-well by addition of EDTA stop buffer (25 mM EDTA, 15 mM Tris-HCl, pH 8.0) and incubated for 30 min at 50 C. Libraries were PCR-amplified using NEBNext High-Fidelity Master Mix and indexed primers (10 cycles), purified by column cleanup, quantified by Qubit, and assessed for nucleosomal patterning by TapeStation.
library_name: 611_highDMSO_r3
identifiers.namespace: SUB15875474
identifiers.id: 611_highDMSO_r3