title: GSM8637536: IMR90 cells treated with exosomes from mutant MEF cells #1; Homo sapiens; RNA-Seq
library_name: GSM8637536
library_construction_protocol: MEF cells were cultured in DMEM 10% exosome-depleted FBS. Exosomes were isolated with a kit (total exosome isolation kit, Thermo). Exosomes were dissolved in PBS and the protein concentrations were determined with a BCA assay. After treatment of cells, total RNA was isolated using Sepazol Reagent (Nacalai) . The sequencing library was constructed with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) and sequencing was performed using NovaSeq 6000 (Illumina). The following procedures such as quality check of the raw paired-end reads, trimming, and alignment to the mm10 mouse reference genome were performed
identifiers.namespace: GEO
identifiers.id: GSM8637536_r1