title: GSM9695017: Control_15D; Mus musculus; RNA-Seq
library_name: GSM9695017
library_construction_protocol: Eyeballs were enucleated, and the retinas were carefully dissected. For single-cell RNA sequencing (scRNA-Seq), the dissected retinal samples were prepared following the 10X Genomics guidelines (#1000414, CG000553, 10X Genomics). In brief, the retinal tissue, separated from the choroid and retinal pigment epithelium (RPE) layer, was fixed in fixation buffer for 24 hours. Following fixation, the retinal tissue was dissociated into a single-cell suspension using dissociation buffer for 20 minutes. The resulting suspension was filtered through a 40 μm strainer to ensure a single-cell population. The samples were processed following the user Guide (CG000691, 10x Genomics) for Chromium Fixed RNA Profiling assay using the Chromium X (10x Genomics). Briefly, between 4,000 and 10,000 cells from the retinal single-cell suspension were captured for each condition after hybridization with Mouse WTA Probes. Libraries were made, checked using High Sensitivity D5000 ScreenTape and the TapeStation 4150 (Agilent), quantified using KAPA library quantification kit, and sequenced into a 10B lane NovaSeq X Plus (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM9695017_r1