title: GSM8797977: S3_SpT; Homo sapiens; OTHER
library_name: GSM8797977
library_construction_protocol: FFPE clinical trial samples were serially sectioned, and one section was H&E stained, and another unstained section was processed for spatial transcriptome analyses using the Visium (10X Genomics). FFPE samples were initially tested for RNA quality with a DV200 > 50% (Agilent). The samples were cut at 5 μM thickness onto four 6.5 mm × 6.5 mm capture areas with 5,000 oligo-barcoded spots of Visium spatial slides. The slides then were deparaffinized and stained by H&E with immediate imaging on EVOS7000 imaging system (Thermo Fisher) and then processed for a full spatial transcriptomic (ST) experiment; cDNA amplification, library preparation, quantification and purity check were performed according to the manufacturer's instructions (10x Genomics, Visium Spatial). Spatial transscriptome sequencing libraries were prepared with 10X Genomics Visium Spatial Gene Expression Slides & Reagent kit according to manufacturer's construction
identifiers.namespace: GEO
identifiers.id: GSM8797977_r1