title: Bulk RNA-seq of untreated human cell lines from multiple tissues
design: Bulk RNA-seq of untreated human cell lines from multiple tissues
library_name: MCF7-6_p
library_construction_protocol: MCF7 and MDA-MB-231 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine. KMBC2 cells were cultured in a 1:1 mixture of DMEM and RPMI supplemented with 5% FBS. Adherent cells were maintained under standard conditions and harvested at ~90% confluence. Jurkat cells were cultured in RPMI supplemented with 10% FBS according to ATCC handling guidelines and maintained between 1 x 10^5 and 1 × 10^6 viable cells/mL. Cells were harvested and processed immediately for RNA extraction. Cells were stabilised in RNA later prior to extraction. Total RNA was isolated using the Monarch RNA purification column system with on-column DNase I treatment, following the manufacturer's instructions. RNA concentration and purity were assessed prior to library preparation. Purified total RNA was submitted to the University of York Bioscience Technology Facility (BTF) Genomics Laboratory for library preparation. Poly(A) RNA was enriched from total RNA and used for stranded library construction according to standard Illumina-compatible protocols. Libraries were indexed and amplified prior to sequencing.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16514:MCF7-6_p