title: GSM8472455: RNA-seq_oocyte_Ctrl_1; Mus musculus; RNA-Seq
library_name: GSM8472455
library_construction_protocol: We followed the methods described previously for smart-seq2 with some modifications.Briefly, oocytes were rinsed in 0.2% BSA/PBS without Ca2+ and Mg2+ and placed in 0.2 ml PCR tube, immediately transferred into cell lysis buffer.After incubation at 72 °C for 3 min, reverse transcription for first-strand cDNA was performed using SuperScript III Reverse Transcriptase.After second-strand cDNA synthesis, the cDNA library was amplified with KAPA Hifi HotStart for 16 cycles. After fragmentation, the sequence libraries were generated using the KAPA Hyper Prep Kit for the Illumina platform, following the manufacturer's instructions. Paired-end 150-bp sequencing was performed on NovaSeq (Illumina) platform.
identifiers.namespace: GEO
identifiers.id: GSM8472455_r1