title: GSM8637715: HDM-BCR; Homo sapiens; OTHER
library_name: GSM8637715
library_construction_protocol: PBMC were isolated from clinical samples and resuspended with 40 mL of PBS for a total of 1 × 108 cells. These cells were then stained using anti-CD19-PE-Cy7, anti-IgG-BV421 and sorted into a 15 ml centrifuge tube by flow cytometry. The single cell suspension (1 × 105 cells/ml) was loaded into the microfluidic devices. Subsequently, the scBCR-seq libraries were constructed according to the protocol of GEXSCOPE Single Cell Immuno-TCR/BCR Kit. Briefly, the magnetic beads with molecular labels captured the poly(A) tail and BCR region on the mRNA to label the cells and mRNA after the cells are lysed. Afterwards, the magnetic beads in the chip were collected, and then mRNAs captured by the magnetic beads were reverse transcribed into cDNA and amplified. The cDNA was enriched for the BCR, and the enriched products were amplified by PCR to construct a sequencing library suitable for the Illumina sequencing platform. Finally, each library was sequenced on Illumina Nova seq 6000 with 150 bp paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM8637715_r1