title: BGB219930 Pre-treatment tumor RNAseq
design: Paired-end libraries were prepared on Beckman BioMek FXp liquid handlers using TruSeq kit reagents. For this, cDNA was A-tailed followed by ligation of the TruSeq UD Indexes (Cat # 20022370) and amplified for 15 PCR cycles following manufacturer‚Äôs recommendation.  AMPure XP beads (A63882, Beckman Coulter) were used for library purification. Libraries were quantified using fragment analyzer and pooled into a pool of 45 samples in equimolar ratios. This pool was quantified using qPCR to determine loading concentration for sequencing. Sequencing was performed on the NovaSeq 6000 instrument using the S4 reagent kit (300 cycles) to generate 2x150bp paired-end reads. The libraries were loaded at an average concentration 250-280 pM and each lane was spiked in with 1% PhiX control library for run quality control. On average, 140 million reads per sample were generated with ~26,370 genes and ~145,330 transcripts detected.
library_name: BGB219930_Pre_tumor_rna
identifiers.namespace: NCI-phs004049
identifiers.id: BGB219930_Pre_tumor_rna