title: GSM9617297: mouse tumor, CD40 & PD1 treated; Mus musculus; RNA-Seq
library_name: GSM9617297
library_construction_protocol: B16 melanoma tumors were mashed through 40-mm filters, and red blood cells were lysed with ammonium-chloride-potassium buffer for 5 min at room temperature followed by washing with phosphate-buffered saline containing 2% fetal bovine serum. Single-cell suspensions from 6 mice per group were pooled and incubated with antibodies against CD16 and CD32, stained with anti-CD45 antibody and SYTOX Blue viability dye. Live CD45+ cells were sorted using a BD FACS Aria I (BD Biosciences). Library preparation were conducted according to 10x Genomics' 5ʹ scRNA-seq guidelines [CG000331_Chromium_Next_GEM_Single_Cell_5-v2_User Guide_RevD].The final libraries were quantified using the Qubit HS dsDNA Assay Kit (Thermo Fisher Scientific), and library profiles were analyzed using High Sensitivity DNA Kit (Agilent).
identifiers.namespace: GEO
identifiers.id: GSM9617297_r1