title: GSM8472206: ATAC_KO_GO_2; Mus musculus; ATAC-seq
library_name: GSM8472206
library_construction_protocol: The growing oocytes (GO) were harvested from 12-day-old WT and Zar1/2 knockout female mice, GV stage oocytes were harvested from 4-week-old WT and Zar1/2 knockout female mice 32-48h after PMSG and the late 1-cell embryos were harvest from 4-week-old WT and Zar1/2 knockout female mice by IVF. All the oocytes/embryos were washed by 0.2% BSA containing PBS for 3 times after the zona pellucida being removed. Samples were used for the ATAC-seq library construction following the protocol of the High-Sensitivity Open Chromatin Profile Kit 2.0 (for Illumina®) (Novoprotein, cat#N248). For extraction in brief, cells were lysed by the lysis buffer for 10 minutes on ice, the washed by wash buffer by centrifuge at 4℃, 500g for 10min, and the nucleus were collected after removing the flow-up. The nucleus were futher used for fragmentation at 37℃, 1000rpm for 30 minutes. After stopping the fragmentation, The DNA were cleaned by beads. Clean DNA fragments were further amplified and indexed in the same PCR reaction.
identifiers.namespace: GEO
identifiers.id: GSM8472206_r1