title: GSM8637529: Retina, Bmal1-mg/-mg, rep4; Mus musculus; RNA-Seq
library_name: GSM8637529
library_construction_protocol: For RNA-Sequencing of microglia and retinal cells, total RNA was isolated from flow-sorted cells using the RNeasy Plus Micro Kit (Qiagen). RNA quantity and integrity was determined using a 2100 Bioanalyzer and RNA Pico kit (Agilent Technologies). All samples (n = 9) contained 2.0 – 4.0ng RNA and had RIN > 8.0 Library preparation was performed by the Genome Technology Access Center (GTAC) through the McDonnell Genome Institute (MGI) with 1-2ng of total RNA per sample. Ds-cDNA was prepared using the SMARTer Ultra Low RNA kit for Illumina Sequencing (Takara-Clontech). Fragments for RNA-Sequencing experiments were amplified and sequenced on an Illumina NovaSeq X Plus using paired end reads extending 150 bases
identifiers.namespace: GEO
identifiers.id: GSM8637529_r1