title: GSM8465824: CRAMP1-KO cells, ATAC, rep2; Homo sapiens; ATAC-seq
library_name: GSM8465824
library_construction_protocol: 50 000 cells per condition were collected by centrifugation and lysed using NP-40, Tween-20 and digitonin to isolate nuclei. Nuclei pellets were incubated with transposase for 30 min before stopping the tagmentation reaction. Libraries were constructed using NEBNext High-Fidelity 2X PCR Master Mix (New Englands Biolabs, M0541S) and ATAC-seq primers (from 2013 PMID: 24097267), and amplified with 7 PCR cycles. DNA was purified and size selected using AMPure XP beads (Beckman Coulter, A63881) ). The quality was checked on a TapeStation 4200 using HS D5000 kits.
identifiers.namespace: GEO
identifiers.id: GSM8465824_r1