title: RNA-seq of fecal culture:in vitro 24h
design: The total DNA from various subgroups of samples was collected through a MicroElute Genomic DNA Kit. The diluted DNA was amplified by the PCR system with the 20 L reaction system using TransStart FastPfu DNA Polymerase (TransGen AP221-02). To analyze bacterial diversity, the universal primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3) were employed to amplify the 16S rRNA gene in the variable area of bacteria V3-V4. Sequencing libraries were created by purifying, calculating, and homogenizing the amplified products. The Illumina HiSeq 2500 platform was used to sequence the qualified library.
library_name: Blank_2
identifiers.namespace: SUB15093909
identifiers.id: Blank_2