title: GSM8660050: HeLa, non-treated, Mnase-IP, YTHDC1 KD, rep2; Homo sapiens; ChIP-Seq
library_name: GSM8660050
library_construction_protocol: For YTHDC1-CLIP, cells were crosslinked by UV and processed with cell lysis and RNase T1 digestion. After immunoprecipitation, the immunocomplexes were digested with protease K and the bound RNAs were purified by using RNA Clean & Concentrator-5 kit (Zymo Research). For m1A-DIP, cells were processed with nuclear extraction and then genomic DNA purification by using DNeasy Blood & Tissue Kit (Qiagen). For MapR, the genomic DNAs were recovered from cell lysates by using MiniElute® PCR purification kit (Qiagene #28004) after digesting with the MNase-conjugated proteins. NEBNext Ultra DNA library kit (for m1A-DIP)
identifiers.namespace: GEO
identifiers.id: GSM8660050_r1