title: GSM8553256: cKO_thymic_iNKT1; Mus musculus; RNA-Seq
library_name: GSM8553256
library_construction_protocol: The thymus from 2 Tbx21Cre Rosa26-YFP+ mice and 2 Tbx21Cre Ets1F/F Rosa26-YFP+ mice were isolated in 10X FBS/PBS and YFP+ cells were isolated on an AriaII flow cytometer. RNA-seq libraries were constructed according the the manufacturers protocol (single cell 3' v2 protocol, 10X Genomics). Briefly, YFP+ cells were resuspended in the master mix and loaded together with partitioning oil and gel beads into the chip to generate the gel based-in-emulsion (GEM). The poly-A RNA from the cell lysate contained in every single GEM was retrotranscripted to cDNA, which contains an Illumina R1 primers sequence, Unique Moleular Identifier (UMI) and the 10X Barcode. The pooled barcoded cDNA was then cleaned with the Silane DynaBeads, amplified by PCR and appropriate sized fragments were selected with SPRIselect reagent for subsequent library consturction. During the library construction Illumina R2 primer sequence, paired-end constructs with P5 and P7 sequences and a sample index were added.
identifiers.namespace: GEO
identifiers.id: GSM8553256_r1