title: BCW isolate 2_W captured on 7-25-2109
design: Nucleic acids were extracted from stored filters using QIAGEN AllPrep PowerViral DNA/RNA extraction kits (QIAGEN, Germantown, MD) according to the manufacturers instructions.  Prior to extraction, filters were transferred to AllPrep PowerViral bead-beating tubes and processed using a MP FastPrep 24 (MP Biomedicals, Santa Ana, CA) at a setting of 4 m/s for 5 min. Following extraction, DNA was quantified using a Qubit High Sensitivity dsDNA quantification kit in a Qubit Fluorometer 4 according to the manufacturer instructions (Invitrogen, Carlsbad, CA).  DNA extracts were stored at -80C. DNA libraries were compiled using the Illumina Nextera library preparation kit following manufacturer instructions.