title: GSM9327101: Jurkat T cell, 132 ORF screen, 90 min stimulation, channel 2, ORF library; Homo sapiens; OTHER
library_construction_protocol: Cells were subjected to the 10x Genomics single-cell ATAC-seq protocol with modifications for the Spear-ATAC (Pierce et al., Nature Communications 2021) amplification of genetic barcodes. ATAC chromatin accessibility libraries were generated following the protocol from 10x Genomics. ORF barcode libraries were generated using the final ATAC libraries as input, as described in the Spear-ATAC protocol, with modifications described in detail in Rubin et al. 2024 bioRxiv.