title: DNA-seq of V. cholerae:Human
design: Briefly, the 4150 Tapestation system was used to determine library size and concentration (Agilent, MA, USA). In order to amplify the tagged DNA and introduce sequencing indexes, the limited-cycle PCR was subsequently employed. We incorporated PhiX Control v3 (Illumina, Inc, San deigo, CA, USA) into each sample prior to library preparation to facilitate a limit of detection assessment for each sample. The prepared libraries were loaded onto a reagent cartridge and subjected to clustering on the NextSeq 2000 System. Subsequently, a paired-end sequencing run with 2  150 bp reads was executed using the NextSeq 2000 platform.