title: GSM8801370: 120DIV_37A4_CO1; Homo sapiens; RNA-Seq
library_construction_protocol: Genomic DNA was extracted from iPSCs using DNeasy® Blood & Tissue Kit (QIAprep #69504) following the manufacturer's instructions Organoids (3 pooled) were harvested and RNA extracted using the Qiagen miRNeasy Mini Kit (Cat. No. 217004) according to manufacturer instructions for all gene expression experiments. The concentration and purity of the RNA samples were measured by using Nano-drop (Thermo Fisher Scientific). The extracted RNA (500 ng) was reverse transcribed according to the protocol supplied with SuperScript III First-Strand Synthesis System for RT (Invitrogen, Cat. No. 18080-051). RIN values were assessed to ensure cDNA library purity and quality. 75 paired-end RNAseq (2x75bp flowcell) was performed by the UTSA genomics core on all 32 samples at the same time with Element Biosciences AVITI DNA Sequencer, using the Freestyle Cloudbreak chemistry at a depth of 25-30 million reads per sample.