title: sgRNA amplicons from Mycobacterium tuberculosis H37Rv_Screen III_20Generation_Post4th_Rec_withATc_rep1 of 2
design: sgRNA guide sequences PCR amplified from genomic DNA preparation of passaging experiment after 20 generations and post incubation in 1st stage and 2nd stage, each incubation for 2 weeks, followed by the 3rd stage for 1 day and the 4th stage incubation for 18 hours plus Atc with recovery in complete 7H9 media