title: GSM9222600: K562 cells; lenti venus_rep1_input;ChIP-seq; Homo sapiens; ChIP-Seq
library_construction_protocol: Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Antibody were added to the sonication samples, followed with gentle rotation for 16 hours at 4°C, Beads added after that,followed with gentle rotation for 6 hours at 4°C. beads transfered to a new tube. Using wash buffer to wash beads, then, samples were incubated in SDS elution buffer for 6 hours. separate beads and supernatant, DNA was in supernatant and then extracted by the phenol/chloroform/isopropanol protocol. Library construction was performed using the DNA Library Prep Kit.