title: GSM9119935: Tibialis Anterior, Aged Dex treatment, 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using Zymo RNA Miniprep kit . 300 nanoograms of total RNA was used for construction of sequencing libraries. A cDNA library was prepared for each sample using the the NEBNext PolyA mRNA and Ultra II RNA library kit for Illumina. Libraries were barcoded for multiplexing with NEBNext Multiplex Oligos for Illumina unique dual index Primer Pairs. The multiplexed sample was quantified with qPCR (Kapa Biosystems) specific for Illumina sequencing primers and the average fragment size was determined with a Tapestation D1000 high sensitivity (Agilent Technologies). The pooled sample was sequenced, with paired-end, 150 base reads on an Illumina NovaSeq X Plus located in the Translational Science Laboratory at the College of Medicine, Florida State University.  The pooled data were demultiplexed into individual sample data.