title: GSM9329938: HL1,rep2; Mus musculus; OTHER
library_construction_protocol: Following reverse crosslinking, DNA was extracted using phenol:chloroform:isoamyl alcohol, and the aqueous phase was transferred to a new tube with glycogen and sodium acetate added. The DNA was then precipitated with ethanol at -20°C for 4 hours, pelleted by centrifugation, air-dried, resuspended in TE buffer, and purified using a DNA Clean & Concentrator kit with final elution in 100 μL. Library preparation was performed on HL-1 cells grown in Claycomb media following the Micro-C-XL method, with the elision of the gel-based sizing of the library (n=2).