title: Whole-genome resequencing of Ciona robusta
design: Genomic DNA was extracted using the DNeasy Blood and Tissue Kit (QIAGEN, Cat. No. 69506) and quantified with a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). Paired-end sequencing targeting approximately 30x coverage was performed on an Illumina HiSeq 4000 platform.