title: RNA-Seq of Anthurium andraeanum spathe
design: Total RNA was assessed for quality prior to library construction. mRNA was enriched using Oligo(dT) magnetic beads for eukaryotic samples or rRNA depletion kits for prokaryotic samples. Enriched mRNA was fragmented using fragmentation buffer. First-strand cDNA was synthesized using fragmented mRNA as template and random hexamer primers. Second-strand cDNA synthesis was performed using buffer, dNTPs, DNA polymerase I, and RNase H. Double-stranded cDNA (ds cDNA) was purified using AMPure XP beads. The purified ds cDNA underwent end repair, dA-tailing, and adapter ligation. Size selection was performed using AMPure XP beads. The adapter-ligated library was amplified by PCR, and the final library was purified using AMPure XP beads.