title: RNA-Seq of Mouse liver
design: Break the RNA to a fragment about 300 bp in length. Choose a fragment with a length of 300 bp because the adapter length is fixed, and if the interrupted fragment is shorter, it will lead to a high proportion of the adapter sequence, thus reducing the proportion of valid data; If the length of the interrupted fragment is long, it is not conducive to the generation of clusters during on-machine sequencing. The first strand of cDNA was synthesized with 10 base random primers and reverse transcriptase using RNA as a template, and the second strand cDNA was synthesized using the first-strand cDNA as a template.