title: GSM8998324: Organoid Day 14 - A; Homo sapiens; RNA-Seq
library_construction_protocol: Pooled organoid were washed in PBS and lysed in RLT buffer with 1% beta-mercaptoethanol. Total RNA was extracted and purified using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA concentration and integrity were assessed using both the Agilent Bioanalyzer and NanoDrop spectrophotometer. Only high-quality RNA samples were used for library preparation. RNA-seq libraries were prepared using the P3 XLEAP 200-cycle kit, targeting a sequencing depth of approximately 1.2 billion reads per run. Libraries were quantified, pooled, and sequenced on the Illumina NextSeq 2000 platform to generate paired-end reads.