title: GSM9005503: Sample 1004 CD45+ cells, TCR; Mus musculus; OTHER
library_construction_protocol: Mice bearing bilateral E0771 breast cancer tumors (5*105 cells/mouse, in 100 uL PBS) were excised following 2 treatments of IT isotype control of 2141-V11. Tumors were excised and homogenized using the genlteMACs dissociation (mouse tumor dissociation kit, Miltenyi) according to the manufacturer's recommendations, then CD45+ immune cells were sorted for analysis. Single cells were encapsulated into nanoliter-scale Gel Beads-in-emulsion (GEMs) using Chromium Controller (10X Genomics) following manufacture's protocol. Chromium Single Cell 5' Library & Gel Bead, Single Cell V(D)J Enrichment Kit, Mouse T Cell, and Single Cell 5' Feature Barcode kits were used to prepare single cell 5' RNA, single cell TCR, and single cell surface protein libraries following manufacture's protocol. The quality and quantity of the libraries were assessed with Agilent TapeStation (Agilent Technologies) and Qubit Fluorometer (Thermo Fisher Scientific).