title: GSM8491788: 32D cell ac4C IP rep2; Mus musculus; RIP-Seq
library_construction_protocol: Total RNA was purified from 32D cells using Trizol (Thermo Scientific) and enrichment of polyadenylated RNA [poly(A) RNA] was achieved through PolyATtract mRNA Isolation Systems (Promega) for acRIP-seq. Then, acRIP was performed as described previously (Cell. 2018 Dec 13;175(7):1872-1886.e24. ) with minor modifications. Libraries were prepared according to NEB's instructions accompanying the NEBNext Ultra II DNA Library Prep Kit (E7103S) and NEBNext Multiplex Oligos for Illumina (E7335S).