title: WGS with PacBio HiFi of Epinephelus itajara: adult male
design: DNA extraction was performed at the Laboratory of Molecular Ecology of Aquatic Vertebrates, Universidad de los Andes (Colombia). The Monarch HMW DNA Extraction Kit for Tissue (NEB #T3060S) was used to obtain high-molecular-weight genomic DNA from the individual selected for whole-genome sequencing. DNA quality and quantity were assessed using Qubit and Nanodrop, and integrity was verified by electrophoresis on a 0.8% agarose gel. Whole-genome sequencing libraries were prepared and sequenced by Macrogen Inc. (Seoul, South Korea). High-fidelity (HiFi) long-read sequencing was performed using the PacBio Revio System following standard protocols.