title: RNA-seq of fibro-infammatory progenitors and  mesothelial cells in the epididymal adipose tissue of high fat diet fed animal treated with Vehicle or Nintedanib+Celecoxib (C3H background)
design: RNA-seq of fibro-infammatory progenitors and  mesothelial cells in the epididymal adipose tissue of high fat diet fed animal treated with Vehicle or Nintedanib+Celecoxib (C3H background)
library_construction_protocol: Mouse epidydimal adipose tissue was harvested and digested in DMEM and collagenase A. After homogenization, the suspension is washed in PBS buffer containing bovine serum albumin (1%) and EDTA (0.5%). After centrifugation, the adipocyte fraction is discarded and the stroma vascular fraction passed through a 100 µm filter before staining.Cell suspensions were stained with appropriate antibodies for 30 min on ice.Cell sorting was performed on a BD FACSAria II™ cell sorter. RNAs from sorted cells were prepared using the RNeasy Plus micro kit (Qiagen). cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche).