title: 16S rRNA gene of scarab larvae gut microbiota
design: Amplification of the V3-V4 hypervariable region of the 16S rRNA (301 pb paired-end) gene using primers S-D-Bact-0341b-S-17 (5-CCTACGGGNGGCWGCAG-3) and S-D-Bact-0785-a-A-21 (5-GACTACHVGGGTATCTAATCC3) (Herlemann et al., 2011); libraries were with the Herculase II Fusion DNA Polymerase and the Nextera XT Index V2 kit.