title: miRNA-Seq of tick cells
design: Total RNA was extracted from SFTSV- or SFV-infected cells using TRIzol LS, with small RNA enrichment and methylation profiling performed via -elimination assay. Infected BME/CTVM6 and AF525 cells were processed at various time points post-infection. Periodate-treated RNA was purified using borate buffer and the Monarch RNA Cleanup Kit prior to UMI small RNA sequencing (DNBSEQ, SE50). Additional samples included RNA isolated from anti-V5 immunoprecipitated complexes to assess viral protein-RNA associations. IP eluates were digested with proteinase K and processed using TRIzol. All libraries were constructed from 500 ng RNA with a minimum yield of 20 million clean reads.