title: Sample pool
design: PacBio HiFi long-read sequencing was performed on a pool sample of integron amplicons obtained with the primers: intI1-R [GCGAACGARTGBCGVAGVGTGTG] +3CS[AAGCAGACTTGACCTGA] and intI3F [AGTGGGTGGCGAATGAGTG] + class3R [CGTCAAACGGGTAAGCAGT]. Twelve monthly samples were amplified with each primer and then pooled. Forward primers already had unique barcodes associated. Only one library preparation was performed (the barcodes inserted in library prep are: 5 end: CGCATGACACGTGTGTT; 3 end: AACACACGTGTCATGCG). The sample specific barcodes (are only present at the 5'end) are as follows:January_int1:ATCACG,February_int1:CGATGT,March_int1:TTAGGC,April_int1:TGACCA,May_int1:ACAGTG,June_int1:GCCAAT,July_int1:CAGATC,August_int1:ACTTGA,September_int1:GATCAG,October_int1:TAGCTT,November_int1:GGCTAC,December_int1:CTTGTA,January_int3:AGTCAA,February_int3:AGTTCC,March_int3:ATGTCA,April_int3:CCGTCC,May_int3:GTAGAG,June_int3:GTCCGC,July_int3:ACTGAT,August_int3:ATGAGC,September_int3:ATTCCT,October_int3:CAAAAG,November_int3:CAACTA,December_int3:CACCGG