title: Endophyte free Ryegrass plant with GA3 applied
design: Fungal communities associated with soil samples were characterised by sequencing the ITS1 region within the fungal ITS rRNA gene with an Illumina MiSeq instrument (paired end, 2  250bp) (Illumina, USA). Sequencing was performed on the 48 soil samples from the two copies of the same 24 plant genotypes selected for transcriptome analysis. The DNA extraction was performed on 500 mg of fresh soil samples using the FastDNA Spin Kit for Soil (MP Biomedicals, USA). DNA purity and concentration were determined with NanoPhotometer spectrophotometer (IMPLEN, USA). Libraries for sequencing were prepared by Macrogen Inc. (Korea) using the primers ITS5F: CTTGGTCATTTAGAGGAAGTAA/ITS2R: GCTGCGTTCTTCATCGATGC.