title: GSM8974298: Control-1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from treated and control HT-29 cells using TRIzol reagent (Thermo Fisher Scientific, USA) according to the manufacturer's protocol. RNA was purified with DNase I treatment and its quality was assessed by NanoDrop and agarose gel electrophoresis. The library was prepared by random fragmentation of cDNA samples followed by 5' and 3' adaptor ligation.