title: GSM9117976: IRF9-Untretaed; Homo sapiens; ChIP-Seq
library_construction_protocol: CUT&RUN assays were performed to profile chromatin-bound transcription factors in Pa01C pancreatic cancer cells using the CUT&RUN Assay Kit (Cell Signaling Technology, Cat# 86652S), following the manufacturer's instructions. Sequencing libraries were prepared using the DNA Library Prep Kit for Illumina Systems (Cell Signaling Technology, Cat# 56795S), including end-repair, adapter ligation, PCR amplification with Multiplex Oligos (Cat# 47538S), and size selection with AMPure XP beads (Beckman Coulter, Cat# A63881).Final libraries were pooled and sequenced using paired-end 75 bp reads on an Illumina NovaSeq X Plus platform.