title: Whole genome sequencing: rabbit feces
design: Genomic DNA was extracted from harvested cells obtained by the overnight culture of B. subtilis YT883 in liquid NB medium using a DNA Purification Kit in accordance with the manufacturers instructions, and purified genomic DNA was quantified by TBS-380 fluorometer (Turner BioSystems Inc., Sunnyvale, CA) and over 1 g genomic DNA of high quality was sent to Shanghai Majorbio Bio-pharm Technology Ltd., China for library preparation and sequencing. DNA samples were sheared into smaller than 400 bp fragments using a Covaris M220 Focused Acoustic Shearer following manufactures protocol. Illumina sequencing library was constructed using NEXTflexTM Rapid DNA-Seq kit (Bioo Scientific Cor. Austin, TX, USA). Paired-end sequencing (2  150 bp) was performed on Illumina Hiseq X Ten machine (Illumina Inc., San Diego, CA, USA) using HiSeq X Reagent Kits according to the manufacturers recommendations.Raw sequence data generated from Illumina platform were used for bioinformatics analysis.Firstly,high-quality data were obtained after quality control was performed by quality cutting, which were then used for de novo assembly using the SOAP denovo v2 to obtain genome sequences. Protein-coding genes(CDSs) were predicted using Glimmer (http://ccb.jhu.edu/software/glimmer/index.shtml), tRNAscan SE v2.0http://trna.ucsc.edu/software/ for tRNA prediction, and Barrap for rRNA prediction.Finally, six major databases (NR, Swiss Prot, Pfam, GO, COG, KEGG) were used for general function annotation to obtain functional information of B. subtilis YT883 genome.