title: Raw reads: ebp-nor_exp2_CalVul_01 _inflorescence
design: This project provides the diploid genome assembly ddCalVul5.1 of the common heather, Calluna vulgaris. The sample was provided by Thomas Marcussen, and the data generation and assembly were carried out by EBP-Nor (https://www.ebpnor.org/). ddCalVul5.1 is based on 46x PacBio HiFi data and 122x Arima Hi-C data. The HiFi reads were filtered for adapters using HiFiAdapterFilt, and assembled with hifiasm along with Hi-C data to generate a pair of haplotype-resolved assemblies. K-mers from the two assemblies were used to filter Hi-C reads to create two sets of reads for scaffolding the assemblies (i.e., reads used to scaffold hap1 did not contain k-mers unique to hap2). YaHS was used for scaffolding. FCS-adaptor was used to remove putative adapters, and putative contaminants were removed with FCS-GX. The chromosomes confirmed by Hi-C data are named according to size.
library_construction_protocol: Arima High Coverage HiC (Hi-C+) with modified circulomics nuclei isolation