title: In vitro model of human derived lung fibroblasts WI-38 stimulated with LPS
design: In vitro model of human derived lung fibroblasts WI-38 stimulated with LPS
library_construction_protocol: Lysis of cells was performed on plate after removal of medium and 1x wash with PBS.  RNA RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany). 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.