title: RNA-Seq of mus musculus: male wound tissue
design: PolyA+ mRNA was isolated via Oligo(dT)-conjugated magnetic beads, fragmented to ~300bp, and reverse-transcribed. Double-stranded cDNA synthesis included end-repair and 3'-A-tailing for adapter ligation. After adapter ligation, the fragments were purified and size-selected, followed by PCR amplification.