title: Metagenomics of Physcia stellaris
design: DNA samples were prepared for whole-genome sequencing using the Illumina DNA Prep tagmentation kit (#20060059) with Illumina Unique Dual Indexes.Sequencing was performed on the Illumina NextSeq2000 platform using a 300-cycle XLEAP-SBS flow cell kit to produce 2x150bp paired reads. 1-2% PhiX control was spiked into the run to support optimal base calling.Read demultiplexing, read trimming, and run analytics were performed using DRAGEN v4.2.7, an on-board analysis software on the NextSeq2000.