title: GSM9327093: Tbx5 KO, Left ventricle, Replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were mechanically homogenized in TRIzol Reagent (Invitrogen), chloroform was added, aqueous phase was isolated, ethanol was added, and then RNA was isolated using RNeasy Mini Columns. Overall, 12μg of total RNA for each sample was treated with the mouse GlobinClear kit (Ambion) to remove the majority of globin RNA Total RNA was prepared with a TRIzol-based extraction and isolated using a Qiagen RNeasy Mini Column