title: sequences of * gene
design: This study presents a comprehensive 7 years evaluation of GM maize expressing cry1Ab/cry2Aj and G10evo-EPSPS proteins through metagenomic and metabolomic analyses. We assessed the effects of GM maize consumption on gut microbiota diversity and metabolite profiles in Cynomolgus macaques (Macaca fascicularis) compared with non-GM maize. Three diet regimens were implemented: a conventional compound feed (CK group), diet formulation containing 70% non-GM maize (Corn group), and diet formulation containing 70% GM maize (Tg group).Feeds that met the nutritional needs of primates were provided to different groups of animals: GM maize feed, non-GM feed, and conventional feed. The monkeys were fed three meals per day, with 110130 g/day of feed per meal. They had access to sufficient drinking water, and fresh fruit was supplemented every 2 days in appropriate amounts. After 6 months of feeding, male and female monkeys were housed together for mating. The breeding colony used was a harem system aligned with the natural social structure of primates, with a male-to-female ratio of 1:4. After an extended period of cohabitation, the animals mated and successfully produced 45 offspring in the next generation (F1), with a gestation period of approximately 165 days. The F0 feeding cycle lasted for 7 years. The F1 generation was continuously fed the same diet as the parents after weaning. In all experimental projects, all F0 generations (8-9 monkeys in each group, for 26 monkeys) and 10 monkeys in each group of the F4 generation were selected as test objects.