title: sequencing of the whole genome of SARS-CoV-2
design: The viral RNA was reverse-transcribed into cDNA and then was synthesized double-stranded DNA. Next, the DNA libraries were prepared using TruePrepTM DNA Library Prep Kit V2 for Illumina (Vazyme, TD503). Agilent 2100 was used for quality control of the libraries showing the peaks near 350-bp. The cDNA libraries concentration was measured by Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific Inc.). Then the sequencing was performed on Illumina NovaSeq 6000 system to 150-bp pair-end with 300 bp cycles Reagent Kit.