title: ddRAD-seq of Crotalus willardi: CLPT0275
design: Double digest restriction-site associated DNA sequence (ddRAD-seq) library preparation followed protocols outlined in DaCosta & Sorenson (2014; also see Lavretsky et al. 2015), but with fragment size selection following Hernandez et al. (2021). Briefly, genomic DNA was enzymatically fragmented using SbfI and EcoRI restriction enzymes, and Illumina TruSeq compatible barcodes were ligated to the sticky ends generated for demultiplexing purposes. The libraries were quantified, pooled in equimolar concentrations, and the multiplexed library was sequenced on Illumina HiSeq X using single-end 150 bp chemistry at Novogene (Novogene CO., Ltd., Sacamento, CA, USA).