title: RNA-seq of apis mellifera: adult forager bee throrax
design: RNA-Seq was performed on flight muscle samples from Apis mellifera honey bees collected from National Chung Hsing University bee farm. Total RNA was extracted using a column-based method with on-membrane DNase I treatment. 1 g of total RNA was used for library preparation. Poly(A) mRNA was isolated using Oligo(dT) beads and then fragmented. cDNA synthesis was performed using random primers for first-strand synthesis, followed by second-strand synthesis. Double-stranded cDNA underwent end-repair, A-tailing, and adaptor ligation. Libraries were size-selected using DNA Clean Beads and PCR-amplified with P5 and P7 primers. Libraries were multiplexed and sequenced on an Illumina NovaSeq instrument using a 2x150 paired-end (PE) configuration.