title: GSM8869831: anti-CD40+Delta24-RGD_3; Mus musculus; RNA-Seq
library_construction_protocol: After six days of treatment, total RNA was isolated from XFM tumors using the RNeasy® Mini Kit (Qiagen) according to the manufacturer's instructions. The RNA concentration and quality were measured using a Qubit HS RNA Assay Kit (Thermo Fisher Scientific) and 4200 TapeStation with High Sensitivity RNA ScreenTape (Agilent Technologies). RNA samples showed good quality, with RIN values ranging from 7.6 to 8.2. Library preparation was performed with 100 ng of total RNA using the Illumina Stranded mRNA Prep Ligation kit (Illumina) following the manufacturer's instructions.