title: PRV_ATAC_12h_Rep1
design: The libraries were constructed using approximately 50,000 cells per sample. Briefly, nuclei were extracted using a lysis buffer containing NP-40 and Triton X-100. Tagmentation was performed using a transposase reaction mixture (Yingzi Gene) at 37°C for 1 hour. The transposed DNA was purified and subsequently amplified by PCR for 9 cycles using NEBNext High-Fidelity 2X PCR Master Mix. The final libraries were purified and size-selected using KAPA Pure Beads. Library quality was assessed on an Agilent 2100 Bioanalyzer, and sequencing was performed on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads.