title: Single-cell RNA-Seq and TCR-Seq of Homo sapiens: CD8+ memory T cells from healthy donor 2
design: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy human donors by Ficoll-Paque density gradient centrifugation. CD3 memory CD4 and CD8 T cells were enriched using magnetic-activated cell sorting (MACS) to reduce contamination from non-T cells. Approximately 10,000 cells per sample were loaded for single-cell immune profiling using the Chromium Next GEM Single Cell Immune Profiling v2 Kit (10x Genomics). Single-cell RNA sequencing and TCR V(D)J profiling libraries were prepared according to the manufacturers protocols. Libraries were sequenced to generate paired-end reads for both gene expression and TCR repertoire analysis.