title: Low coverage genome sequencing of crayfish: pereopod muscle
design: Extracted DNA was fragmented using the Bioruptor Pico sonication device (Diagenode, Hologic Inc., Liege, Belgium) for 21 cycles of 30 s ON followed by 30 s OFF. Illumina libraries were prepared according to the BEST protocol (Care et al., 2018). Preparation of PCR reactions was automated using a Biomek i7 Hybrid workstation (Beckman Coulter, Brea, CA, USA). Library lengths were verified on a TapeStation system (Agilent Technologies, Santa Clara, CA, USA). Libraries of all species were barcoded, pooled, and sequenced on an Illumina NovaSeq 6000 to obtain 2 x 150 bp paired-end reads.