title: Perturb-seq of GPCRs in primary keratinocytes, using 10x genomics technology. This sample is gene expression RNA sequencing.
design: Perturb-seq using 10x genomics sequencing; this file contains the gene expression reads (single cell RNA-seq) for the well indicated by the last digit of the number in the sample_ID. Cells were differentiated for zero, three or six days with calcium and high density. Cell harvesting followed the 10x Genomics protocol for Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing. 40,000 cells per well were added to 10X Genomics Chromium chips and libraries were prepared using the Chromium Single Cell 3' gene expression and feature barcode library kits (RevA, RevB, 10x Genomics) according to manufacturer's instructions.