title: 5R-16S rDNA sequencing of Homo sapiens: melanoma tumor and control tissues
design: Samples were collected and processed using the CTAB method. Negative controls, including sampling, DNA extraction, and no-template PCR amplification controls, were included to account for contamination at various stages.16S rRNA gene amplification targeted 5 regions in multiplex. The libraries were sequenced on the Illumina NovaSeq 6000 system. Reads were demultiplexed, filtered, and aligned to the amplified regions. SMURF method was used for profiling. GreenGenes (May 2045 version) was used as a reference. Contamination was detected and removed following the specified filters.