title: SHR12wF4 (CRX1158764; CRA018341)
library_construction_protocol: Briefly, total genomic DNA was extracted using the FastDNA SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA) according to the manufacturer's instructions. The integrity of genomic DNA was detected through agarose gel electrophoresis, and the concentration and purity of genomic DNA were detected through the Nanodrop 2000 and Qubit3.0 Spectrophotometer. The V3-V4 hypervariable regions of the 16S rRNA gene were amplified with the primers 341F (5-CCTACGGGNGGCWGCAG-3 ) and 805R (5 -GACTACHVGGGTATCTAATCC-3 ) and then sequenced using Illumina NovaSeq 6000 sequencer.