title: 16S rDNA amplicon sequencing of mouse: mice feces
design: PCR fragments was cleaned with a Gel Extraction Kit (Qiagen, Germany) following the manufacturers specifications and monitored on 2% agarose gel. Sequencing libraries were created using the Preparation Kit (Illumina, USA) according to the manufacturers specifications and index codes were supplemented. Sequencing was performed on an Illumina HiSeq 2500 Cloud Platform (Novogene, Beijing, China) and 250 bp paired-end reads were obtained. Reads were merged via FLASH (v1.2.7) and the sequences were analyzed using Uparse software (v7.0.1004).