title: SSU rRNA amplicon sequencing of arbuscular mycorrhizal fungal communities
design: DNA was extracted from 0.2 g soil samples from each mesocosm using the QIAGEN DNeasy PowerSoil Pro Kit. Amplification of the SSU rRNA gene was performed using a semi-nested PCR approach, with primers AML1/AML2 in the first round and AML1/WANDA in the second round.