title: RNA-seq of wl1 mutant
design: For RNA extraction, unexpanded leaves were excised from at least 10 20-day-old wl1 mutant plants and from the wild-type seedlings segregated from them. This procedure was performed for each biological replicate. Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific) and purified with the RNeasy Mini Kit (Qiagen). RNA sequencing libraries were constructed using the Illumina standard library preparation kit. Three biological replicates were included for both the wild type and the wl1 mutant. RNA sequencing was carried out on an Illumina HiSeq 2500 platform.