title: GSM8697645: Early_Life_RLT_other_LNs; Mus musculus; RNA-Seq
library_construction_protocol: Mouse spleen, mesenteric, and other lymph nodes (cervical, brachial, axillary, inguinal, and para-aortic) were procured and pooled in digestion medium consisting of RPMI 1640 with 2% foetal bovine serum (FBS) (Sigma-Aldrich), with deoxyribonuclease (DNase) (100 µg/mL; Roche) and LiberaseTM (96 µg/mL; Roche), minced and agitated at 37°C with gentle agitation for 30 min, and passed through a 70-micron filter. Cells were then sorted for live, tdTmt+ MHCII+ cells and processed for single-cell analysis. 10x Chromium V3 3' Protocol