title: WGS of Klebsiella pneumoniae: 4 days,  Male, Blood
design: DNA fragments were end-repaired and A-tailed using the NEBNext Ultra II End Repair Kit (NEB), then barcoded with Blunt TA Ligase Master Mix (M0367L). Barcoded samples were pooled equimolarly, and sequencing adapters (SQK-NBD114.96) were ligated using NEB Quick T4 DNA Ligase. After AMPure XP bead purification, libraries were quantified with Qubit and sequenced on the Nanopore PromethION system (P24, FLO-PRO114M) according to library concentration and required data output.