title: ITS amplicon metabrcoding shoe dust
design: For fungal metabarcoding, the ITS1 region was amplified using                 indexed ITS1catta and non-indexed ITS2 primers. Sequencing was conducted using the                 PacBio Illumina NovaSeq 6000 platform. The ITS region was amplified from pure fungal                 cultures using universal primers ITS9mun and ITS4ngs.