title: GSM9477206: DIPGXIII, AZD4573+sertraline, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Tumor samples were isolated by dissociating whole brains, depleting mouse cells, and using the human cell fraction for bulk RNA-seq. Neurospheres were dissociated to single cells with TrypLE Express Enzyme, washed with 1X HBSS, counted, and prepared for CUT&RUN with 250,000 cells/sample. RNA was extracted with Trizol and used to generate cDNA from mRNA. Sequencing libraries were prepared from cDNA following Illumina protocols.