title: 16S rDNA of Amur tiger:Tiger cub fecal
design: Amplicons were extracted from 2% agarose gels, purified using AMPure XP Beads (Beckman Agencourt, USA) according to the manufacturers instructions, and quantified using an ABI StepOnePlus Real-Time PCR System (Life Technologies, Foster City, USA). Purified amplicons were pooled in equimolar amounts and paired-end sequenced (PE254) on an Illumina platform according to standard protocols.