title: Metagenomics of chicken sample from Guinea infected by Infectious Bronchitis virus
design: Amplified ds cDNA was purified using 1.8X AMPure beads and used for library preparation for sequencing with the S5 Next-Generation Sequencing system. 100ng of ds cDNA was enzymatically fragmented into 200 bp lengths using Ion shear Plus reagents and followed by adapters and barcodes ligation. Size selection was performed using Pippin Prep and further amplified for 8 cycles. The amplified barcoded library was loaded onto the Ion Chef Instrument for automated template preparation and chip loading using Ion 540 Kit-Chef. Sequencing was performed on an Ion S5 Next generation sequencing system, with 500 flows to generate 200 bp reads.