title: GSM9327160: Muscle, Control, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Muscle samples were immediately flash-frozen upon biopsy and stored at -80ºC. They were then transported on dry ice to the NIH, where they were uniformly processed. RNA extraction was performed using TRIzol. Libraries were prepared either using the NeoPrep system with the TruSeq Stranded mRNA Library Prep protocol (Illumina, San Diego, CA) or with the NEBNext Poly(A) mRNA Magnetic Isolation Module and Ultra™ II Directional RNA Library Prep Kit for Illumina (New England BioLabs, ref. #E7490 and #E7760).