title: GSM8947404: ComBMP; Homo sapiens; RNA-Seq
library_construction_protocol: Organoid samples were dissociated using TrypLE (Thermo Fisher, Cat. No. 12605010) at 37°C for the following duration: 15 min for Inc IM and Com ; 20 min for An  and Com IM; and 25 min for LI and  SI. Single Cell RNA-seq libraries were prepared using the 10x Chromium iX and GEM-X Single Cell 3' Kit v4 (10x genomics, PN-1000691). Briefly, the cell suspension (target recovery 10,000 cells) was mixed with reverse transcription master mix and loaded with Single Cell 3′ Gel Beads and Partitioning Oil into a GEM-X Single Cell 3' Chip Kit v4 (10x genomics, PN-1000690) to generate single-cell Gel Bead-in-emulsion (GEM).