title: GSM8974717: 31 div; Homo sapiens; RNA-Seq
library_construction_protocol: For each timepoint, two wells of a 4-well chamber slide were dissociated and pooled. The selected protocol involved dissociating with warm TrypLE (Thermo Fisher #12605010) for 25 minutes at 37C, resuspending in FACS buffer at RT (0.02% BSA in DPBS), then propidium iodine was added for 10 minutes (Thermo Fisher #P1304MP) before placing on ice. FACS for single live cells was performed by the UCI Flow Cytometry Core using a FACSAria II (BD Biosciences). Single cell capture with the 10x Chromium platform, cDNA library preparation, and cDNA QC analysis (Qubit dsDNA HS Assay kit (Life Technologies Q32851) and high sensitivity DNA chips (Agilent. 5067-4626)) were performed by the UCI Genomics Research and Technology Hub (GRT Hub). Library generation was done with a V2 chemistry kit and an average capture of ~10,000 barcoded cells, per user guide (CG00052 Rev B). The cDNA libraries were sequenced using the HiSeq 4000 platform (Illumina) to achieve an average depth of 50,000 reads per cell and 2,500 genes per cell.