title: 10X CNV single cell analysis of Lentinula edodes
design: Frozen sperm were sent to GENEWIZ for 10 Genomics single-cell copy number variation (CNV) library preparation and sequencing. The cells were prepared according to a previous single sperm cell analysis study12. A single-cell library was constructed according to the manufacturers protocol. Sequencing was performed in three lanes using a HiSeq 4000 (Illumina) with paired-end sequencing of 150 bp.