title: Zebrafish homogenate, transcriptome
design: Perform transcriptome sequencing, extract total RNA from the sample, digest the DNA with DNase, and enrich eukaryotic mRNA with magnetic beads containing Oligo (dT); Add interruption reagent to break mRNA into short fragments. Afterwards, the TruSeq RNA library Prep Kit v2 (Illumina, San Diego, CA, USA) was used to transform it into a cDNA library. The cDNA library sequencing reaction was performed on the Illumina HiSeq 2500 platform (Illumina).