title: library of functional and non-functional gRNAS in E. coli enriched by Cas9 shifting to permissive temperatures
design: Pool of gRNAs with known activity enriched by Cas9 activity with or without an RNA thermometer at permissive temperatures. Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina with 3 technical replicates. Phased primers were used to introduce heterogeneity to the ends of reads