title: GSM9222623: HUDEP-2 cells,SKI_sg1_rep3, Day0; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA-seq, RNA was extracted from 5 × 10e5 HUDEP-2 cells (with at least two biological replicates in each case) by using an RNeasy Mini Kit (Qiagen) according to the previous protocol. a TruSeq Stranded mRNA Library Prep Kit (Illumina) was used to enrich polyA + RNA and create libraries for sequencing using a HiSeq2000 System (Illumina).