title: leaves sample
design: Single-cell RNA sequencing (scRNA-seq) was performed to profile the transcriptomes of individual cells. Single cells were isolated using 10x Chromium, followed by cDNA synthesis and library preparation using the 10x Genomics Single Cell 3' v3 Kit. Sequencing was carried out on the MGI sequencing platform, and data was processed using Cell Ranger and Seurat to generate gene expression matrices.