title: GSM9034719: RNA in water, isolated from oncostatin-M treated adult mouse cardiomyocytes; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from cultured adult cardiomyocytes with Trizol reagent (Invitrogen), samples pooled and cleaned up with the RNeasy Mini kit (Qiagen, #74104) Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. Library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.