title: ESCC-CAF-shcon2
design: Total RNA was extracted using the TRIzol kit (Invitrogen, Carlsbad, CA, USA). Eukaryotic mRNA was enriched with oligo (dT) beads. Enriched mRNA was then fragmented into short fragments using fragmentation buffer and reverse-transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB#7531, New England Biolabs, Ipswich, MA, USA) and PCR was conducted. The resulting cDNA library was sequenced using Illumina Novaseq X Plus (Repugene Technology, Hangzhou, China).