title: Illumina NovaSeq paired-end sequencing of pSJ77 BAC-phagemid mutated by T7 DNAP v1
design: 200 ug of purified mutated pSJ77 BAC-phagemid DNA was randomly fragmented by Covaris to 300-350 bp. Fragments underwent end repair, 5' phosphorylation, 3' adenylation, and adaptor ligation, followed by size selection via cleanup beads. The library was PCR amplified for 8 cycles using P5/P7 indexing primers.