title: metatranscriptome of Tyrophagus putrescentiae
design: The concentration of total RNA was determined using the Qubit RNA Assay Kit (Life Technologies). 1ug RNA from each sample was used for rRNA removal by using Ribo-Zero Plus rRNA Depletion Kit (Illumina). rRNA depleted samples were quantified and used for library preparation using the KAPA mRNA HyperPrep Kits (Roche) by following the manufacturer's instructions. Following the library preparation, the final concentration of all the libraries were measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies). The libraries were then pooled in equimolar ratios of 0.6nM, and sequenced paired end for 500 cycles using the NovaSeq 6000 system (Illumina).