title: AL8/78 PacBio HiFi sequences
design: HiFi libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 and cleaned with the Enzyme Clean up Kit (Pacific Biosciences, Menlo Park, CA, USA). The libraries were size selected utilizing BluePippin (Sage Sciences, Beverly, MA, USA). HiFi SMRTbell templates longer than 15 kb were collected and cleaned with AMPure PB beads (Pacific Biosciences, Menlo Park, CA, USA). Concentration and final size distribution of the libraries were evaluated using a QubitTM 1X dsDNA HS Assay Kit (Thermo Fisher, Waltham, MA, USA) and Femto Pulse System (Agilent, Santa Clara, CA, USA), respectively. HiFi libraries with approximate insert sizes of 15 kb were independently sequenced on two SMRT cells of a Pacific Biosciences Sequel II instrument