title: GSM9217057: cc_dox free_mouse-2; Mus musculus; RNA-Seq
library_construction_protocol: PSCs from different species were seeded onto MEF-coated plates either cultured separately or mixed at different ratios for co-cultures in NBFR medium. The seeding ratio and density were empirically tested and decided based on cell growth rate. For 4-days-co-culture assays between human-mouse, cells were seeded at a 4:1 ratio at a density of 1.25 × 10-4 cells cm−2. At each of the indicated time points, cell concentration was manually counted and calculated, and the percentages of each cell line were determined using FACS Calibur system (BD Bioscience). Total cell numbers (tN) for each species in co-cultures or separate cultures were determined by multiplying total cell volume (V) with cell concentration (CC) and percentage (P). tN = V × CC × P. Cell density (cells cm−2) was calculated by dividing the total cell number by the surface area. by Novogene