title: Greenhouse experiment DNA sequences collected from soil, sample BNI_GH97, soil biodiversity MED, 1 invasive species, replicate 2
design: A total of 120 soil samples were sequenced. We extracted DNA from soil samples using the PowerMax Soil DNA Isolation Kit and amplified the fungal ITS2 region using the degenerate primer pair fITS7:fITS7o as forward primer and ITS4 as reverse primer. The ITS2 region was sequenced on an Illumina NovaSeq platform using a 2 x 250 bp paired-end sequencing approach by Novogene Company.