title: Regulatory mechanism of HNRNPA2B1 in human esophageal squamous cell carcinoma cells
design: Regulatory mechanism of HNRNPA2B1 in human esophageal squamous cell carcinoma cells
library_construction_protocol: KYSE150 cells (CL-0638, Procell Life Science & Technology Co., Ltd., China) were cultured at 37℃ with 5% CO2 in an RPMI-1640 and Ham's F-12 (PM150110 and PM150810, Procell Life Science & Technology Co., Ltd., China) with 10% fetal bovine serum (FBS) (10091148, Gibco, China), 100 µg/mL streptomycin, 100 U/mL penicillin (SV30010, Hyclone, USA). Cells were infected by lentivirus with MOI=50. Stable cell lines were obtained by screening with 1 µg/mL puromycin and cells were harvested for RT-qPCR and Western blotting analysis 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion). VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries.