title: GSM9144472: Subcutaneous LLC tumors+con IgG-#1; Mus musculus; RNA-Seq
library_construction_protocol: Subcutaneous LLC tumors were harvested on the day after the last administration of the mAbs. Tissues were minced on ice and then digested at 37°C with a shaking speed of 50 rpm or 30 min. Cell suspensions were filtered through a 40-μm strainer, and the cell pellet was resuspended in red blood cell lysis solution and incubated on ice for 5 min. The cell pellet was washed and resuspended in PBS containing 0.5% FBS, followed by the evaluation of cell viability. The samples were then sent to Majorbio Biopharm Technology Co., Ltd. (Shanghai, China), for further sequencing and bioinformatic analysis. Briefly, library construction was performed according to the manufacturer's protocol (DNBelab C TaiM4 V3.0) and quantified via a high-sensitivity DNA chip on a Bioanalyzer 2100 and a Qubit high-sensitivity DNA assay (Thermo Fisher Scientific). Libraries were sequenced on the MGI DNBSEQ-T7 platform (MGI-Tech, Shenzhen, China).