title: GSM8490585: KJC335, cellobiose, biol rep 1; Acetivibrio thermocellus DSM 1313; RNA-Seq
library_construction_protocol: All cultures were harvested for total RNA purification at the mid-log phase (e.g., OD600 between 0.45 – 0.50).  To harvest the cells for total RNA purification, it was first chilled by swirling the anaerobic culture in dry ice/70% ethanol for 45 seconds and kept on ice for 5 minutes. The inactivated cells (1 – 1.5 mL) were then pelleted by centrifugation. The supernatant was discarded, and the cell pellets were lysed with lysozyme-EDTA using a Qiagen RNeasy Mini kit (Cat No./ID: 74104) following the manufacturer's protocol. Each sample was treated with DNaseI (Qiagen, Cat No./ID: 79254) twice following the protocol to remove the genomic DNA in the total RNA completely. Performed by commercial service through Genewiz