title: GSM9582968: CAF_2; Rattus norvegicus; miRNA-Seq
library_construction_protocol: At dissection, a piece of liver tissue was cut into small pieces, placed in Ambion RNAlater solution (Thermo Fisher Scientific Inc., Massachusetts, USA), and stored overnight at 4 °C. The following day, the samples were transferred to -20 °C until use. Total RNA, including the small RNA fraction, was isolated from 10–20 mg of liver tissue using the miRNeasy Mini Kit (Qiagen GmbH, Hilden, Germany) according to the manufacturer's instructions. The miRNA-seq library was prepared using the QIAseq miRNA Library Kit (Qiagen), following the manufacturer's instructions. In brief, a pre-adenylated DNA adapter was ligated to the 3' ends of all miRNAs, followed by ligation of an RNA adapter to the 5' ends of mature miRNAs. Reverse-transcription (RT) was then performed using an RT primer containing Unique Molecular Indices (UMIs). Finally, the library was amplified with sample-specific index primers.