title: RPE1_CBE_gene_Cis_lib_screen
design: The genomic DNA of each replicate was extracted from RPE1 cells using the DNeasy Blood & Tissue kit (Qiagen) and the sgRNA regions were PCR amplified by KAPA Hot Start High-Fidelity DNA Polymerase with 26 cycles using indicated primers. The PCR products were purified with DNA Clean & Concentranter-5 (Zymo Research Corporation), followed by NGS analysis