title: Nave, cre+, 16s-4
design: Dispense 2.5 uL of Index 1 primers into columns 112 and Index 2 primers into rows AH of a 96-well rack, then cap and store remaining primers at 20 C. To each well, add 12.5 uL 2x high-fidelity polymerase mix, 5 uL water, and 2.5 uL 16S product. Seal, spin at 1,000 g for 1 minute, and run PCR (95 C 3 min; 8 cycles of 95 C 30 s, 55 C 30 s, 72 C 30 s; 72 C 5 min). Confirm the ~630 bp product by chip or 1.5 percent agarose gel.