title: GSM9144960: P027 tumor, de novo; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from tumor tissues using the Qiagen RNeasy Mini Kit according to the manufacturer's protocol. RNA concentration and purity were assessed using NanoDrop, and integrity was evaluated using the Agilent Fragment Analyzer 5200 to obtain RIN scores. Library preparation and sequencing were performed by the MCW Linda T. and John A. Mellowes Center for Genomic Sciences and Precision Medicine. Poly(A)-enriched mRNA libraries were constructed using the Illumina TruSeq Stranded mRNA Library Prep Kit, and sequencing was conducted on the Illumina NovaSeq 6000 platform with paired-end 100 bp reads.