title: Transcriptomic profiling of the pig limbic and endocrine tissues
design: Transcriptomic profiling of the pig limbic and endocrine tissues
library_construction_protocol: Animal handling and compassionate euthanasia were performed in compliance with pertinent ethical laws, standards, and regulations. The study included 8 female German Landrace pigs, with an average age of 170 ± 14 days and weight of 105 ± 8 kg. The adrenal gland and brain tissues, including the amygdala, hippocampus, hypothalamus, pituitary gland, and thalamus were swiftly removed, flash-frozen in liquid nitrogen, and preserved at − 80 °C for subsequent analyses.  Tissue sample was finely grounded in liquid nitrogen into powder. Total RNA was purified using the RNeasy Mini Kit (Qiagen, Germany) and treated with DNase I (Roche Diagnostics, Mannheim, Germany) to remove trace DNA contamination. The RNA quantity and purity were assessed using a NanoDrop ND-1000 spectrophotometer (Peqlab) and the RNA integrity was determined using the RNA 6000 Nano Kit for 2100 Bioanalyzer System (Agilent Technologies). DNA libraries were generated using the Illumina Stranded mRNA preparation kit (Illumina, San Diego, CA, USA).