title: GSM9223159: heart tissue, IR_24h_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the mirVana miRNA Isolation Kit(Ambion) followingthe manufacturer's protocol. 4 μg of total RNA was used to construct sequencing libraries. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number(RIN)≥ 7 were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (llumina, San Diego, CA, USA) according to the manufacturer's instructions. Then these libraries were sequenced on the llumina sequencing platform.