title: RNA-Seq of raw milk: milk 3
design: Raw milk samples provided by a dairy processing plant were subjected to DNA extraction using the eGENE-UP PCR system. The quality and quantity of the extracted DNA were assessed using the ND 1000 spectrophotometer NanoDrop and the Qubit dsDNA HS assay, respectively. Amplification of the 16S rRNA gene (V3-V4 region) was performed using a two-step dual-indexed PCR protocol, and sequencing was conducted on Illumina platforms. Purification and quality checks were carried out using agarose gels, a Bioanalyzer, and spectrophotometric assays.