title: Raw B159
design: Library Preparation & Sequencing: gDNA samples were used as the input for library preparation using Library Preparation Kit from Oxford Nanopore Technologies (ONT). gDNA was repaired using end prep enzyme mix, generating DNA with 5 phosphorylated, 3 dA-tailed ends. The repaired DNA was ligated with ONT-compatible Adapter. The library was quantified with Qubit Fluorometer before loading to the Flow Cell. Sequencing was performed using GridION (Oxford Nanopore Technologies) until the desired yield has achieved. GridION sequencing was operated by MinKNOW software. Base calling was performed using Dorado with high accuration mode (HAC).