title: RNA seq from GBM cell lines and primary cultures after irradiation
design: RNA seq from GBM cell lines and primary cultures after irradiation
library_construction_protocol: Parent GBM cells were grown to 70% confluency in vitro and subjected to their respective lethal doses of radiation U87MG (10Gy) and SF268 (8Gy). Cells were monitored for their SA-β galactosidase activity as gold standard for senescence detection and samples were harvested at the maximum SA-β gal positive population that is RS, when they lost senescence phenotype (EOS) and the regrown population thereof as Relapse (R). Total RNA was isolated from cells using RNA Isoplus (Takara). Total mRNA was enriched from parent, RS, EOS and Relapse GBM samples and processed for library preparation. Libraries were generated following the TruSeq RNA library protocol (Illumina). In summary, 4 μg of intact total RNA underwent mRNA purification through oligodT beads, and library preparation adhered to the manufacturer's guidelines using the TruSeq RNA Sample Preparation Kit (Illumina).