title: GSM8800251: Human CD45+ sorted from FL_NUP98-NSD1_derived xenograft cells_rep2; Homo sapiens; ATAC-seq
library_construction_protocol: Xenografted cell were isolated from bone marrow. Human cells were enriched using mouse depletion kit (Miltenyi Biotec) and human CD45+ cells were sorted by flow cytometry (BD FACSMelody). On average, 3-5x10^5 cells per sample was submitted for scRNA-seq (10x Genomics Chromium Next GEM Single Cell ATAC Reagent Kit v1.1). Viability of single cells was assessed using Acridine Orange/Propidium Iodide viability staining reagent (Nexcelom), and debris-free suspensions of >80% viability were deemed suitable for experiments. The 10x Genomics Chromium Next GEM Single Cell ATAC Reagent Kit v1.1 was used for scATAC-seq.