title: GSM8641842: BC_cfDNA_BeforeTreatment_6; Homo sapiens; ATAC-seq
library_construction_protocol: NEXFLEX Rapid DNA-Seq Kit 2.0 was used to prepare cfDNA libraries. Approximately 650 µL of blood plasma from either cancer patients or healthy donors was processed for cfDNA extraction using the SubX™ Exo-DNA Kit (Capital Biosciences). The isolated cfDNA was quantified with the Qubit dsDNA High Sensitivity (HS) Assay Kit. Libraries were prepared using the NEXTFLEX Rapid DNA-Seq Kit 2.0 (Revvity). Half the volume of purified cfDNAs, ranging from approximately 1 ng to 10 ng, was utilized. After adapter ligation, a total of 11 PCR cycles were performed.