title: RNA-seq
design: Ribosomal RNA (rRNA) was depleted from mixed bacterial RNA samples using the RiboCop rRNA Depletion Kit (Lexogen), optimized for prokaryotic transcriptome profiling. Stranded mRNA libraries were constructed with the Illumina Stranded mRNA Prep Kit following the manufacturers protocol then subjected to Illumina NovaSeq 6000 platform to generate approximately 9 Gb of raw data per sample