title: GSM9444466: 1A.007.stroma; Homo sapiens; RNA-Seq
library_construction_protocol: ROIs were segregated using a nuclear counterstain and anti-cytokeratin immunofluorescence probes (clone AE1/AE3, Novus NBP2-33200) to separate RNA captured from tumor epithelium (cytokeratin-positive) from RNA in the adjacent SIME (cytokeratin negative). Aspirates from 93 ROIs were collected (25 benign cervix, 21 Silva A, 22 Silva B, and 25 Silva C) Library preparation was performed according to the manufacturer's instructions. During the library preparation process, the photocleaved oligonucleotides with tag sequence identifiers, unique molecular identifiers, and sequencing primer reads were PCR amplified. Unique dual indexes and flow cell sequences were added. Library concentration was quantified by Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Libraries were sequenced on the Illumina NovaSeq X Plus (Illumina, Inc., San Diego, CA, USA), targeting 1.5 billion reads per library.