title: Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice
design: Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice
library_construction_protocol: Panicles which were in the panicle differentiation stage were microscopically isolated. WT and GR2-Ri rice seeds were sterilized with 2.5 % sodium hypochlorite for 15 min and washed extensively with distilled water. These seeds were germinated in Petri dishes with wetted filter papers at 37  C in the dark. After 48 h incubation, uniformly germinated seeds were selected and cultivated in a beaker containing Kimura B nutrient solution. The hydroponically cultivated seedlings were grown in a Phytotron (Agricultural Experimental Station, National Taiwan University, Taipei) with natural sunlight.  Total RNA was isolated  from microscopically isolated panicles (panicle at differentiation stage) using the TRIZOL reagent (Invitrogen), according to the supplier's recommendations. A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina