title: KAS-Seq Input of MCF7 cells with ARID1A KO
design: The KAS-seq protocol was performed based on previous studies (25,13). MCF7 parental and ARID1A -/- cells were treated with 5 mM N3-kethoxal (Generon Ltd, Cat No. A8793-25) for 10 minutes on a 12-well plate. 10,000 cells were harvested after treatment, and genomic DNA was isolated using the PureLink DNA kit (Life Technologies Ltd., Cat. No. K182001). The labelled ssDNA was isolated using Biotin and streptavidin-based purification (Thermo Scientific Ltd, Cat No. 65001). The Illumina Tagment DNA TDE1 buffer and enzyme kit (Illumina, Cat. No. 20034197) was used to prepare the libraries via a transposition reaction with Nextera adapters (NEB, Cat. No. M0544S).