title: Soil sample from Streptomyces against RKN (G1)
design: To explore the bacterial communities of each treatment through an Illumina NGS, total DNA was extracted from 3 replicates of each treatment by using a TIANamp Soil DNA Kit (Tiangen Biotech, Beijing, China). The DNA quantity and quality were determined using a DS-11FX+ spectro/fluorometer (DeNovix, Wilmington, DE, USA). The V4 variable region of the 16S rRNA gene was amplified using a pair of primers, 515F and 806R, linked to the barcodes  and a Phusion high-fidelity PCR master mix (New England Biolabs, Ipswich, MA, USA). The PCR product was purified using a QIAquick gel extraction kit (Qiagen, Inc., Valencia, CA, USA). The amplified DNA library was constructed with a TruSeq DNA PCR-free sample preparation kit (Illumina, San Diego, CA, USA) and analyzed with a HiSeq2500 PE250 sequencing platform (Illumina, San Diego, CA, USA).