title: GSM8491297: HT1080 cells (+/-), IDH1 WT expression, tumor collected 33 days after cell injection, sample 32; Mus musculus; RNA-Seq
library_construction_protocol: RNA samples were extracted from 22 tumor tissues using QIAzol Lysis Reagent. 100–200 mg of the tumor tissue was placed in gentle MACS M tubes. 500 µL of QIAzol Lysis Reagent was added to the tissue, and the samples were dissociated mechanically using a gentle MACS Dissociator following manufacturer's protocol Libraries were generated using the Illumina Ribo-Zero Plus rRNA Depletion Kit with IDT for Illumina RNA UD Indexes following manufacturer's protocol. Generated libraries were multiplexed and sequenced with 150 base pair (bp) Paired End (PE150) to a depth of approximately 25 million reads per sample on an Illumina NovaSeq 6000. Samples were finally demultiplexed using bcl2fastq v2.20 Conversion Software