title: GSM9329163: BMDM_Adcy7KO_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRI Reagent (Sigma Aldrich, US) from BMDMs. The integrity of the RNA was confirmed using a Bioanalyzer 2100 (RIN > 7.0) and agarose gel electrophoresis. Poly (A) RNA was purified using Dynabeads Oligo (dT) 25 and fragmented with the Magnesium RNA Fragmentation Module. Reverse transcription was then performed with SuperScript™ II. Adapter ligation to A-tailed cDNA fragments was performed following end repair, and size selection was conducted using AMPure XP beads. The ligated products were then amplified via PCR, and the final cDNA library had an average insert size of 300 ± 50 bp.