title: GSM8798882: HFD1-KO4; Mus musculus; RNA-Seq
library_construction_protocol: Adipose progenitors defined as CD45-CD31-PDGFRb+ were flow sorted and frozen following RNeasy RNA Plus micro Kit (Qiagen) instructions to extract RNA. Quality of the RNA was measured using a Bioanalyzer 2100 (Agilent) as per the manufacturer's instructions using pico chip kit, RIN > 6,5 were kept for sequencing. Non-strand-oriented libraries were prepared following the NEBNext Single Cell/Low Input RNA Library Prep Kit (New England BioLabs)