title: GSM8491201: livers from tumor-bearing WT mice, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Cell preparation protocol: Livers were harvested from the indicated mice and snap frozen in liquid nitrogen. The frozen livers were ground into a powder. Equal amounts of the powdered liver tissue were lysed in RNA extraction buffer and then processed for RNA extraction. Total RNA were extracted using RNAeasy mini-prep kit from Qiagen The integrity of total RNA samples were assessed on Bioanalyzer 2100 using RNA 6000 Pico chip (Agilent Technologies, CA).  Messenger RNA was enriched from these samples using QIAseq FastSelect - rRNA HMR Kit (Qiagen, CA), fragmented and converted to cDNA and Illumina sequencing library using the PrepX RNA-seq library preparation protocol on the Apollo 324TM NGS Library Prep System (Takara Bio, CA).