title: GSM9327049: Ctl_8hr_Rep1; Homo sapiens; ATAC-seq
library_construction_protocol: 5e4 cells were pelleted and resuspended in 50ul of ATAC RSB with 0.1% NP40, 0.1% Tween-20, and 0.01%. After three minutes, 1ml of ATAC RSB with 0.1% Tween-20 was added, tubes were inverted, and nuclei were centrifuged at 500 rcf for 10 min. Supernatant was carefully removed and nuclei were resuspended in 50ul transposition mix (25ul TD buffer, 2.5ul transposase, 16.5ul PBS, 0.5ul 0.1% digitonin, 0.5ul 10% Tween-20, and 5ul water). Transposition was performed for 30 minutes at 37 C with shaking in a thermomixer at 1000 RPM. Reactions were purified with a Zymo DNA Clean & Concentrator 5 kit. Libraries were generated largely following the Omni-ATAC protocol (Corces et al., 2017, Nature Methods). Purified ATAC fragments were amplified for eight cycles and purified using SPRIselect beads (1.2x). Libraries were quantified by Qubit and Bioanalyzer before sequencing on an Illumina NextSeq 500 targeting 2e6 read-pairs per library.