title: GSM9327397: Supermono10 TILs, scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: F1 mice were inoculated s.c. with 5 × 10⁵ KP1 cells and received i.p. administration of mono-IL-10 or IL-10/2-Trikine (3 μg functional cytokine per dose) every other day from day 7 to day 19, along with anti-PD-1 antibody (BioXcell, 200 μg per dose, clone RMP1-14) on days 7, 11, 15, and 19. On day 19, mice were sacrificed and tumors were collected. Tumor-infiltrating leukocytes were enriched by density gradient centrifugation using Ficoll-Paque PLUS (Cytiva), followed by surface marker and DAPI staining (Thermo Fisher Scientific). Live CD45⁺ leukocytes were sorted using a Sony SH800 Cell Sorter (Sony Biotechnology) for Single cell RNA sequencing. 10x 3' Gene Expression v3.1