title: single-cell RNA sequencing of effector memory CD8+ T cells of nasal-associated lymphoid tissue (NALT), spleen, submandibular & cervical lymph nodes and blood of Epstein Barr Virus (EBV)-infected NSG mice engrafted with a human immune system (HIS mice)
design: single-cell RNA sequencing of effector memory CD8+ T cells of nasal-associated lymphoid tissue (NALT), spleen, submandibular & cervical lymph nodes and blood of Epstein Barr Virus (EBV)-infected NSG mice engrafted with a human immune system (HIS mice)
library_construction_protocol: flow cytometry assisted single-cell sorting of single cell suspension derived from organs of HIS mice intranasal EBV infection of humanized mice with 10^5 RGU of Luciferase-carrying EBV in 20ul (applied in both nostrils under anesthesia) RNA is directly extracted from cells and hybridized on 10x barcoded beads with an oligo dT tail Single Cell 3' v3.1 protocol was followed as single index protocol and as detailed in the Chromium Single Cell 3' Reagent Kits User Guide - v3.1 Chemistry.