title: GSM9329355: Uninj-WT 1; Mus musculus; RNA-Seq
library_construction_protocol: Mice were placed in an induction chamber with inhalation of isoflurane through a nasal tube. The artery was ligated in two loops using a 7-0 monofilament polypropylene suture proximal to the internal-external carotid bifurcation. Surgical procedures were conducted aseptically, and the surgical incision was closed with a 3-0 non-absorbable silk suture. Carotid samples were collected at 7 days (N=9 WT and N=9 c-Kit haploinsufficient male mice) after occlusion. To minimize the number of animals used, the contralateral carotid artery of each animal was used as a control. Libraries were generated using depleted RNA obtained from 1 μg of total RNA with a TruSeq Sample Preparation RNA kit (Illumina, Inc., San Diego, CA, USA), according to the manufacturer's protocol. All libraries were sequenced on Illumina HiSeq 1000, generating paired-end reads of 100 bp.