title: PacBio CLR Sequencing
design: DNA polymerase was bound to the complex using the DNA/Polymerase Binding Kit P6. Subsequently, MagBead Kit was used to bind the library complex to magnetic beads, which enabled long-read sequencing. The polymerase-bound SMRTbell library was then loaded into zero-mode waveguides (ZMWs). The SMRTbell library was sequenced using 261 SMRT cells (Pacific Biosciences) with C4 chemistry (DNA Sequencing Reagent 4.0), and each cell captured 240-minute movies using the PacBio RS sequencing platform.