title: Dm-aKG treated Intestinal stem cells NEBNext Enzymatic 5hmC-seq (E5hmC-seq) sequencing and data processing
design: Dm-aKG treated Intestinal stem cells NEBNext Enzymatic 5hmC-seq (E5hmC-seq) sequencing and data processing
library_construction_protocol: ISCs from 6 PBS and 6 dm-aKG treated mice were sorted  total DNA was isolated using Quick-DNA microprep kit(Zymo). DNA was fractionated using Bioruptor NGS(Diagenode)  after adding control DNAs from the NEBNext Enzymatic 5hmC-seq Kit(NEB-E3350S) to 250 ng of sample. The sonicated DNA libraries were constructed using NEBNext Primers for Epigenetics(Dual Index Primer Pairs Set 2B)(NEB-E3392S).