title: Hybrid assembly: PacBio Whole Genome Sequencing of Klebsiella pneumoniae
design: The DNA of the multidrug-resistant Klebsiella pneumoniae were extracted using DNeasy Blood and Tissue Kit (QIAGEN). A 10-kb library of the purified DNA was processed using SMRTbell template prep kit 1.0 (Pacific Biosciences, Menlo Park, CA) and sequenced on one singlemolecule real-time (SMRT) II cell. A whole genome sequence was performed using hybrid assembly; PacBioscience and Illumina technology.