title: leaf sample
design: Next-generation sequencing library construction and sequencing: DNA samples underwent random fragmentation using the Covaris ultrasonic crusher. The Illumina sequencing library was prepared using the Nextera DNA Flex Library Prep Kit (Illumina, San Diego, CA, USA) following a series of steps including the DNA fragments with a target insertion fragment size of 150 bp were subjected to terminal repair, A-tail addition, sequencing splice addition, purification, and PCR amplification.