title: OpcochT00R1: Opuntia cochenillifera RNA-Seq at 0 h (06:00 AM, dawn)  Replicate 1
design: Samples were obtained from fully mature cladodes, collected at 7 time points over a 24-hour period (every 4 hours) and 3 replicates per time point throughout the light/dark cycle (diel time) under greenhouse conditions. cDNA libraries were prepared from the Opuntia cladode samples, and sequencing was carried out on the Illumina NovaSeq6000 platform using 16 flow-cell channels. Indexed reads were de-multiplexed (Illumina) and output in FASTQ format. The raw FASTQ files were processed to remove Illumina adapters and filtered for low-quality reads (Q < 20) using Trimmomatic (Bolger et al., 2014). Approximately 229 Gbp of raw data were generated, resulting in a total of 162 Gbp of trimmed sequence reads.