title: WGS of Klebsiella pneumoniae: clinical isolate DA-39-2
design: Genomic DNA was extracted from pure bacterial cultures using the [Tn Kit, e.g., QIAamp DNA Mini Kit]. DNA concentration and purity were assessed using a Qubit 4.0 Fluorometer. Sequencing libraries were prepared using the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) according to the manufacturers protocol. The libraries were pooled and sequenced on the Illumina MiSeq platform using a 300-cycle V2 reagent kit, generating 2x150 bp paired-end reads.