title: GSM9445025: KRT5 positive SKOV3 cells, rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol reagent according to manufacture's instructions (Invitrogen). RNA integrity and concentrations were determined using Nanodrop and only the purified RNA samples were used for further analysis. ERCC Spike in in Mix 1 (Thermo Fisher) was added to samples prior to library creation according to manufacturer's recommendation. 3' mRNA-seq libraries were created using QuantSeq 3' mRNA-Seq library prep kit according to the manufacturer's instructions using 10ng total RNA.