title: Ganoderma lucidumC2
design: Using non parametric transcriptome sequencing, prepare a PE library according to the VAHTS Universal V6 RNA seq Library Prep Kit for Illumina (Vazyme) instructions. Randomly cleave mRNA and sequentially complete the synthesis of the first and second strands of cDNA. After processing the end effector, specific sized fragments were screened using AMPure XP beads, and finally a complete cDNA library was prepared by PCR amplification. Evaluate library quality using Agilent Bioanalyzer 4150.