title: GSM9329131: Col_input rep1; Arabidopsis thaliana; ChIP-Seq
library_construction_protocol: Materials were ground with liquid nitrogen into fine powder and fixed with 1% formaldehyde.To profile H3K36me3, nuclei were extracted and mononucleosomes were generated with micrococcal nuclease (MNase)(Sigma, N5386) digestion. Immunoprecipitation was conducted with  anti-H3K36me3 (Abcam, ab9050). After antibody incubation, Protein A Dynabeads (Life technologies, 10002D) were added to collect the immunocomplexes, followed by elution, reverse cross-linking and DNA purification. Immunoprecipitated DNA were subjected to library preparation with the VAHTS universal DNA library prep kit for Illumina (Vazyme, ND607-02) according to the manufacturer's instruction