title: GSM9504036: ER-associated RNA replicate 1; Homo sapiens; RNA-Seq
library_construction_protocol: ER fractions were isolated by ER immunoprecipitation (ER-IP) in the presence of RNase inhibitor. RNA was extracted from ER-IP material and from whole-cell lysates using TRIzol reagent according to the manufacturer's instructions. RNA was precipitated with isopropanol, washed with 70% ethanol, and resuspended in nuclease-free water. RNA libraries were prepared using an Illumina-compatible RNA-seq library preparation kit according to the manufacturer's instructions and sequenced on an Illumina platform.