title: RNA Sequencing of Th17 cells treated with ckba
design: CD4+ naive T cells were seperrated from spleen of IL-17-GFP mice and cultured in IMDM supplmented with  10% fetal calf serum , 1% (v/v) penicillinstreptomycin , 55M -mercaptoethanol , 1 mM sodium pyruvate, 2 mM glutamine and 5mM Hepes in humidified atomsphere.and differentiated toward to Pathogenic th17 cells  and treated with dmso or ckba(8M) for three days.