title: ="eDNA-metabarcoding"&" "&"of"&" "&"hyporheic"&" "&"macroinvertebrates:"&" "&B50
design: DNA was extracted from filters of sterile distilled water sample (filtration control) and the mitochondrial cytochrome oxidase subunit 1 (COI) gene was amplified using PCR and sequenced using Illumina MiSeq