title: GSM9328265: Antigen-specific GC B cells, GT8-IL-21-NIC DNA vaccine; Mus musculus; OTHER
library_construction_protocol: Lymph nodes collected from VH1-2RJH2/VK1-33RCSd/hTdT mice and antigen-specific germinal center B cells sorted by FACS 10x Genomics Chromium single-cell 5' V(D)J library prep (GEM-X Single Cell 5' V3 Kit) according to manufacturer's protocol; cDNA generated and amplified; sequencing adaptors ligated; double-sided SPRIselect bead clean-ups