title: sequnences of bacteria
design: This study involved the collection of fecal samples from mice. Total DNA was extracted from the fecal samples using a commercial DNA extraction kit. The 16S rRNA gene V3-V16 region was amplified using PCR with specific primers. PCR products were purified and used to construct sequencing libraries. Sequencing was performed on an Illumina platform to generate high-throughput sequencing data.