title: GSM8901429: SLAMseq_KMS11_dTAGparental_dTAGv1_0d_Rep2; Homo sapiens; OTHER
library_construction_protocol: Cells were metabolically labeled with 100 µM 4-thiouridine (4sU) for 1 hour. Total RNA was extracted using TRIzol reagent followed by purification with the RNA Clean & Concentrator-5 kit (Zymo Research). 10 µg of RNA was then alkylated with iodoacetamide, followed by another cleanup using the RNA Clean & Concentrator-5 kit. 1 µg of the resulting RNA was used as input for library construction. Sequencing libraries were prepared from 1 µg of alkylated total RNA using the QuantSeq 3'mRNA-Seq Library Prep FWD Kit for Illumina (Lexogen #015), following the manufacturer's protocol.