title: WFDBR22020821
design: A metabarcoding survey to determine the fungal community composition in the North InletWinyah Bay (NI-WB) estuary in South Carolina, USA, in the summer and winter of 2020 and 2021. To sequence the V4-V5 region of the 16S and 18S rRNA genes, the 515F-Y (5'-GTGYCAGCMGCCGCGGTAA-3') and 926R (5'-CCGYCAATTYMTTTRAGTTT-3') universal primers were used (Parada et al., 2016). The LR0R (5'-ACSCGCTGAACTTAAGC-3') and LF402 (5'-TTCCCTTTYARCAATTTCAC-3') primers were used to sequence the 28S LSU region of fungi (Tedersoo et al., 2015). Lastly, the ITS3tagmix (Supplementary Table S1) (Tedersoo et al., 2015) and ITS4tag001 (5'-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGACGAGTGCGTTCCTSCGCTTATTGATATGC-3') (Tedersoo et al., 2014) primers were used to sequence the ITS2 region.