title: WGS of Phyllospadix iwatensis
design: High - quality genomic DNA was extracted from plant tissues. Then, devices such as the Covaris ultrasonic disruptor were used to shear it into fragments with a length range of 2050 kb. Reagents like the SMRTbell Template Prep Kit were employed to perform end - repair on the sheared DNA fragments. Subsequently, circular single - stranded DNA adapters were ligated, and an SMRTbell library was constructed.