title: GSM9086021: wild type Plasmodium falciparum 3D7 strain, schizont stage, control 2; Plasmodium falciparum; RNA-Seq
library_construction_protocol: synchronization of the parasites was achieved by isolating late schizonts via 45%/75% Percoll gradients [91], followed by treatment with 5% sorbitol [92], which facilitated the establishment of tightly synchronized ring-stage cultures over a 3-h window. mRNA was enriched using poly-T oligo-attached magnetic beads. Reverse transcription was performed with SuperScript II (Invitrogen) using random primers. The reaction was then treated with RNaseH to remove the RNA template and cleaned using DNA Clean and Concentrator Kit (Zymo Research). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumia) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced using the Illumina platform.