title: GSM9220080: p134 T cells (day 0) scTCR-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Peripheral blood mononuclear cells (PBMCs) were isolated; B cells were positively selected (CD20 beads, Miltenyi). Single-cell suspensions of CD20⁺ B cells and CD4 T cells were prepared at 1,000 cells/µL in 0.04% PBS-BSA, with >90% viability by trypan blue. Samples were transferred to the Yale Center for Genomic Analysis (YCGA) for 10x Chromium processing and sequencing. 10x Genomics Chromium Single Cell 5′ chemistry was used to generate single-cell 5′ gene-expression libraries.