title: 16s-rRNA of red hybrid tilapia: probiotics C2
design: Total genomic DNA was extracted from 250 mg of homogenised tilapia intestine material (n = 8) by using commercial DNA isolation kit, QIAamp PowerFecal Pro DNA kit (Qiagen, Hilden, Germany). Sequencing was performed by AGTC Genomics company (Kuala Lumpr, Malaysia). DNA samples were amplified via PCR using primer pair 341F/805R (341F: 5'-CCTACGGGNGGCWGCAG-3', 805R:  5'-GACTACHVGGGTATCTAATCC-3') specific for V3-V4 hypervariable region [24], followed by cleaned up using AMPure XP beads. The library was constructed according to the Illumina standard protocol (Part # 15044223 Rev. B). Each sample were sequenced with approximately 200,000 reads, 250 base pair in length paired end (2 x 250 base pair) on Illumina Miseq sequencing platform (Illumina Inc., California, USA).