title: RNA-Seq of  Oryza sativa: JN100 Control BR1
design: Leaf samples were collected from Oryza sativa cv. JN100 under control conditions. Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and  purity was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.