title: GSM8491720: BT-474, anti-ZNF410, WT, rep2, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde at room temperature, followed by quenching with glycine. After cell lysis, the chromatin was sonicated for 100-500 bp at 4°C. ChIP-seq libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina from Vazyme. The purified DNA was end-repaired and then ligated with adapters. After purified by AMPure XP beads from Beckman, the DNA was amplified using 13 cycles with the PCR program.