title: DNA-Seq of Homo sapiens: lymph node
design: Genomic DNA was extracted from 99 FFPE lymphoma tissue samples using the MagMAX FFPE DNA/RNA Ultra Kit (Thermo Fisher Scientific, #A31881, Waltham, MA, USA). Libraries were prepared using the onAmpliSeqTM DNA library Kit (Thermo Fisher Scientific). The DNA input was 10 ng/pool, 20 ng in total, and library preparation guidelines for 2 x 2-primer pool panel were followed. Libraries were quantified by qPCR and the Ion Chef system was used for template preparation and chip loading. A maximum of 9 samples were included in the Ion 550 chip in a single run that is expected to achieve an output of 12 million reads per sample. Chips were sequenced on the Ion Torrent Ion GeneStudio S5 Prime System (Thermo Fisher Scientific).