title: 4T1_PBS_1 (CRX798208; CRA012733)
library_construction_protocol: To systematically identify the changes in the gene expression of key molecules after combination therapy in 4T1 tumors. Flash-frozen tumors (n=3 mice per treatment group) were pulverized, and total RNA was extracted and an RNA-seq library was constructed. RNA-seq data were generated on an Illumina NovaSeq 6000 using the 150-bp pair-ended running mode. After removing reads with sequencing adaptors, unknown reads with "N" and low-quality reads, clean reads were mapped to a reference genome.