title: Plas
design: Libraries can be constructed from total RNA (1 ng - 2 g) or purified Small RNA using the NEXTFLEX Small RNA-SEq Kit v3. The process includes 3 'and 5' connector connection, reverse transcription and PCR amplification (12-25 cycles). The purification method is determined based on the number of PCR cycles and the presence of linker dimers (~130 bp) : for 18 cycles and no dimers, magnetic bead gel-free purification can be adopted. Otherwise, the PAGE glue needs to be cut and purified. Sequencing data should first have the connectors removed and the random bases at both ends removed, and then be compared and analyzed.