title: RNA-seq to investigate rewiring the transcriptome of castration-resistant prostate cancer through CDK9 inhibition dataset 2
design: RNA-seq to investigate rewiring the transcriptome of castration-resistant prostate cancer through CDK9 inhibition dataset 2
library_construction_protocol: Snap-frozen tissue fragment RNA was extracted using RNeasy® Mini Kit (74106, Qiagen) from tissue deriving from 8 different mice NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences. 