title: Microbial communities in Lagoa de Furnas
design: A total volume of 900 mL of pooled sample water was filtered using 0.22m pore bottle-top vacuum filter system (KASVI). Total genomic DNA was extracted with DNeasy PowerWater kit (QIAGEN), followed by an additional step of clean up using DNeasy columns (QIAGEN). Sequencing library was prepared from 450ng of genomic DNA with the Rapid DNA Sequencing kit (SQK-RAD004) acquired from Oxford Nanopore Technologies (ONT). DNA sequencing was performed using the minION device (ONT) with the FLO-MIN111 flow cell.