title: Rhizosphere soil from mock-treated *Brassica oleracea*, 16S rRNA sequencing: low aphid density experiment
design: Rhizosphere soil DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen). The V3-V4 region of the 16S rRNA gene was amplified using primers 341F (5-CCTACGGGNGGCWGCAG-3) and 805R (5-GACTACHVGGGTATCTAATCC-3). Amplicon libraries were sequenced at a minimum depth of 100,000 paired-end reads (2  250 bp) per sample on the Illumina NovaSeq 6000 platform by Genome Quebec.