title: HiFi genomic reads of Lentinula edodes
design: Genomic DNA was extracted from fruit bodies of XR1 with CTAB method (Doyle and Doyle 1990 ) and purified with DNeasy PowerClean Pro Cleanup Kit (Qiagen). The DNA was subjected to HiFi SMRTbell library construction using the SMRTbell Express Template Prep Kit 2.0 (PacBio), according to the manufacturers instructions, with a minor modification. Because the genomic DNA was degraded, the DNA shearing step recommended in the protocol was skipped. The resultant DNA was fractionated with BluePippin (Sage Science) to eliminate fragments less than 15 kb in size. The DNA library was sequenced on a single SMRT cell 8M on the Sequel IIe system (PacBio).