title: Illumina HiSeq X paired end sequencing of SAMD00891620
library_construction_protocol: Total DNA was extracted using the QIAamp Fast DNA Stool Mini Kit (QIAGEN, N.V., Hilden, Germany). The concentration and purity of the total DNA solution were measured with a NanoDrop 2000 (Thermo Scientific, Waltham, MA, USA) and an Invitrogen Qubit 2.0 Fluorometer (Thermo Fisher Scientific). DNA extract quality was checked on 5% agarose gel. A metagenomic library with an insert size of 300 bp was constructed from high-quality DNA extracted from each sample using the NEBNext Ultra II DNA Library Preparation (New England Biolabs, Ipswich, MA, USA), the dual index primers set of NEBNext Multiplex Oligos for Illumina (New England Biolabs), the AMPure XP Beads (Beckman Coulter, Brea, CA, USA) following the manufacturer's manual instructions. The Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, USA) was used to determine fragment size distribution. Metagenomic sequencing was performed using 150-bp paired-end reads on the Illumina HiSeq X platform (Illumina, San Diego, CA, USA).