title: Cherokee Brave HiFi Run 1
design: Cherokee Brave flower buds were collected from a residential tree in Knoxville, TN, U.S. and frozen in a -80C freezer. Bionano Prep Plant Tissue DNA Isolation kit (Bionano Genomics, San Diego, CA, U.S.) was used to isolate nuclei from the bud tissue. High molecular weight (HMW) DNA was extracted from the nuclei with Circulomics Nanobind Plant Nuclei Big DNA Kit (Pacific Biosciences, Menlo Park, CA, U.S.). HMW DNA was sheared to ~15kb fragments with the Covaris g-TUBE (Woburn, MA, U.S.). The sheared DNA was used to prepare a PacBio SMRTbell library and size selected using BluePippin (Sage Science, Beverly, MA, U.S.). The library was sequenced on three Sequel IIe (Pacific Biosciences, Menlo Park, CA, U.S.) cells.