title: GSM9221195: D1; Mus musculus; RNA-Seq
library_construction_protocol: Following euthanasia, the colon was excised and washed with PBS containing 1 mM Phenylmethanesulfonyl fluoride (PMSF) and 0.2 mM sodium orthovanadate. Measurements of colon length and weight were taken, and sections of the colon were sampled for RNA extraction. An mRNA sequencing library was prepared from 5 μg of total RNA, and its concentration was quantified using the Qubit fluorometric quantitation method. Sequencing was performed on the HiSeq X10 system