title: 16S rRNA gene ampl seq
design: Libraries were prepared by following Illumina 16S Metagenomic Sequencing Library Preparation protocol in two amplification steps: an initial PCR amplification using locus-specific PCR primers and a subsequent amplification that integrates relevant flow-cell binding domains and unique indices (NexteraXT Index Kit, FC1311001/FC1311002). Libraries were sequenced on NovaSeq6000 instrument (Illumina, San Diego, CA) using 250-bp paired-end mode.