title: RNA sequence analysis of Arabidopsis thaliana : the role of mRNA methylation in control of seed germination
design: Total RNA was isolated from Col-0 seed 20 hours after imbibition using TRI Reagent (Sigma) followed by chloroform separation and isopropanol precipitation. Poly(A) mRNA was enriched with oligo(dT) beads, fragmented, and converted to strand-specific Illumina libraries via random-primed cDNA synthesis, end-repair, A-tailing, adapter ligation, size selection (250300 bp inserts), and limited-cycle PCR. Libraries were sequenced as 2  150 bp paired-end reads on an Illumina platform (Novogene, Q30  88%). The complete experiment was performed once with three biological replicates per genotype.