title: Dovetail LinkPrep HiC library (3/4) and Illumina NovaseqX 2x150 sequencing of the GIAB HG008-T tumor cell line (Pancreatic Ductal Adenocarcinoma)
design: LinkPrep library preparation was performed starting with 1x10^6 non-viable HG008-T cells (2024 NIST passage 21) using the Dovetail Genomics LinkPrep kit (21025V, v2.0, https://cantatabio.com/wp-content/uploads/2025/02/Dovetail_LinkPrep_Kit_User_Guide_version-2.0_Mammals-1.pdf).  The cells were prepared following the mammalian cell line sample preparation protocol (Stage 1A)  for crosslinking and in situ tagmentation.  Proximity ligation, crosslink reversal and DNA purification was then performed. Purified crosslinked reversed DNA from the 1x106 cells was split evenly for four separate library preparations, data here is from one of the four libraries prepared.  Libraries were dual-indexed, purified and size selected for the final library size range of 350 - 1,000bps. The resulting four LinkPrep libraries were then pooled for sequencing on an Illumina NovaseqXplus with 2x150 paired end reads using the Illumina NovaSeq X Series 25B Reagent Kit (300 Cycle) (PN: 20104706). The pooled library was loaded onto one lane of the flowcell.