title: Spatial transcriptomics of human colorectal cancer liver metastatic samples
design: Spatial transcriptomics of human colorectal cancer liver metastatic samples
library_construction_protocol: Metastatic colorectal cancer tissue was provided by the Barts Cancer Tissue Bank. We accessed archived frozen tissue samples and collected fresh tissue samples from the Royal London Hospital. Fresh tissue samples were flash frozen in a dry ice/ethanol bath and stored at -80°C. 10 µm sections were taken from fresh frozen liver metastatic CRC samples from three patients. The tissue permeabilisation time was optimised using a Visium Spatial Tissue Optimization Slide & Reagent Kit (10x Genomics, 1000193), following the manufacturer's instructions (Rev D). A 6 minute permeabilisation time was selected.  Spatial transcriptomic libraries were created using a Visium Spatial Gene Expression Slide & Reagent Kit (10x Genomics, 1000187), following the manufacturer's instructions (Rev E).