title: GSM9443851: OE33-788-Adar-N173S-41-IFNB-1; Homo sapiens; RNA-Seq
library_construction_protocol: After RNA isolation, oly-A selected mRNA libraries were sequenced on Illumina HiSeq4000 yielding 20-30x106 150 b.p. paired end reads per sample. Total RNAs were extracted using RNeasy mini kit (Qiagen) according to the manufacturer's instruction. Libraries were generated using the QIAseq 1-Step Amplicon Library Kit. mRNA was enriched via poly-A selection, followed by cDNA synthesis, adapter ligation, and PCR amplification.