title: library of functional and non-functional gRNAS in E. coli enriched by Cas9 at non-permissive temperatures
design: Pool of gRNAs with known activity enriched by Cas9 activity with or without an RNA thermometer at non- permissive temperatures. Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina with 3 technical replicates. Phased primers were used to introduce heterogeneity to the ends of reads.