title: GSM9503586: LV, BZ, patch, Day3, rep3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Each snap frozen heart tissue (timepoint; Day3, 1wk, 4wks, tissue; IZ, BZ, RZ for each group) were homogenized in Buffer RLT by disrupter MS-100R (TOMY). Total-RNA was extracted using RNeasy Mini Kit (74106, Qiagen) according to the manufacturer's instructions. The quality of purified RNAs was assessed by Bioanalyzer 2100 (Agilent Technologies). Library preparation was performed using a TruSeq stranded mRNA sample prep kit (RS-122-2101, Illumina) according to the manufacturer's instructions.