title: GSM8640193: PoolD_REP1_T0; Homo sapiens; OTHER
library_construction_protocol: gDNA from cell pellets was isolated using QIAGEN Gentra Puregene kit. Genome-integrated sgRNA sequences were amplified by PCR using KOD Hot Start Polymerases. i5 and i7 multiplexing barcodes (Ilumina) were added in a second round of PCR and final gel-purified products were sequenced.