title: GSM9444157: Donor 3, Unexposed; Homo sapiens; RNA-Seq
library_construction_protocol: Isolated cells were resuspended in buffer PKD (Qiagen) and incubated with proteinase K (0.1 μg/sample) for 1 hour at 50°C. Cells were then lysed using TriPure™ Isolation Reagent. Samples were subseqeuntly processed for bulk RNA sequencing using the RNA Sequencing PLUS workflow by Single Cell Discoveries (Utrecht, The Netherlands). Libraries were constructed using the RNA sequencing PLUS workflow by Single Cell Discoveries. Libraries were sequenced to a depth of approximately 20 million raw reads per sample using single-end 75 bp (1×75) chemistry on an Illumina NovaSeq X Plus.