title: GSM8472698: Cohort 2-Control-PLX; Mus musculus; RNA-Seq
library_construction_protocol: Mice mice underwent CO2 asphyxiation and were perfused with phosphate buffered saline (PBS). The hippocampus was dissected and dissociated using the Miltenyi Adult Mouse Brain Dissociation Kit per manufacturer's instructions with minor modifications. Tissue was immediately placed into Enzyme P solution (37°C), Enzyme A solution was added, and tissue was dissociated in C-tubes using a gentleMACS dissociator. Density centrifugation was used to remove myelin debris, remaining red blood cells were lysed, and cell viability and concentration was determined using a hemocytometer. Hippocampi from three mice per group were pooled into each sample. This experiment was performed in two replicates, with six mice per group total, and approximately 3,000-5,000 cells were recovered from each sample. Single-cell suspension was loaded onto a 10X chip and run on a Chromium Controller to generate gel-bead emulsions. Protocols for UMI barcoding, cDNA amplification, and library construction followed the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1 kit protocol (10x Genomics; 1000121). Library quality was determined by Agilent High Sensitivity DNA BioAnalyzer chip and was sequenced using an Illumina HiSeq.