title: RNA-Seq of Infected Silurus glanis: Skin
design: European catfish (Silurus glanis L. 1758) mRNA was enriched using oligo(dT) beads after removal of rRNA using Ribo-Zero kit. The mRNA was then randomly fragmented and used to synthesize cDNA using random hexamer primers. Second-strand synthesis was performed using custom second-strand synthesis buffer (Illumina), dNTPs, RNase H, and DNA polymerase I. After a series of terminal repair, A-ligation, and sequencing adaptor ligation, the double-stranded cDNA library was completed through size selection and PCR enrichment.