title: GSM8698385: GA_ctrl_1; Homo sapiens; RNA-Seq
library_construction_protocol: On day 19, RNA was isolated using Qiagen RNeasy Micro Kit according to the manufacturer's instructions. RNA libraries for RNA-seq were prepared using BGI in house mRNA-Seq Library Prep Kit following manufacturer's protocols (sample QC, mRNA Isolation, mRNA Fragmentation, cDNA Synthesis, End Repair, Add A and Adaptor Ligation, PCR, Library QC, Circularization). Libraries were paired-end sequenced with a read length of 100 bp and an average depth of 20 million reads.