title: GSM9222677: RAM_BAL_KO_naive_rep2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from freshly sorted cells using micro RNA kit (Qiagen, Germantown, MD), following manufacturer's protocols. RNA purity, quantity, and integrity were determined by NanoDrop (ThermoFisher Scientific, Waltham, MA) and TapeStation 4200 (Agilent, Santa Clara, CA) analyses prior to RNA-seq library preparation. MGIEasy RNA Library Prep Set (3.1, cat. 1000006384) was used to generate libraries with an input of 100 ng of total RNA.