title: GSM9003711: Adult Kidney rep2; Mus musculus; ChIP-Seq
library_construction_protocol: Nuclei were extracted from kidneys with a homogenizer in hypotonic buffer. Chromatin from released nuclei was crosslinked with 1% formaldehyde for 10 min. After quenching and nuclear lysis, chromatin was sonicated using a Bioruptor Pico (Diagenode). ChIP-seq libraries were prepared using the MicroPlex kit v2 (Diagenode) according to the manufacturer's protocol. 1ng of immunoprecipitated material was repaired, end-blunted and amplified (10 cycles). The resulting libraries were purified using AMPure XP beads protocol (Beckman Coulter, Indianapolis, IN). Libraries were then normalized and pooled at 2nM and spike-in PhiX Control v3 (Illumina, San Diego, CA) was added. Sequencing was done using a NextSeq 500 instrument (Illumina) in paired-end read mode (2x40 cycles).