title: GSM9581392: E14.5 control, GEX; Mus musculus; RNA-Seq
library_construction_protocol: Live CD45- TER119- lung cells were isolated by FACS from mutant and littermate control mice at E14.5 and E17.5. Multiome samples were prepared following the 10x Genomics protocol [CG000366 Rev D]. Briefly, 50,000-100,000 sorted cells were incubated in 0.1X Lysis Buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 0.1% Nonidet P40 Substitute, 0.001% Digitonin, 1% BSA, 1 mM DTT, 1 U/μL RNase inhibitor in nuclease-free water) on ice for 3 min. The samples were washed three times with Wash Buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1 mM DTT, 1 U/μL RNase inhibitor in nuclease-free water), then resuspended in Diluted Nuclei Buffer prior to library preparation. Libraries were prepared using the 10x Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kit.