title: GSM8491760: ddx4E4Δ_ovary_rep2; Danio rerio; RNA-Seq
library_construction_protocol: Ovaries were dissected from WT and ddx4-E4Δ zebrafish at 6 mpf. Total RNA was extracted using the Trizol reagent according to the manufacturer's protocol. RNA quality was determined by 2100 Bioanalyser (Agilent) and quantified using the ND-2000 spectrophotometer (NanoDrop Technologies). RNA-seq libraries were constructed using the TruSeqTM RNA sample preparation Kit (Illumina, San Diego, CA). About 1μg of high-quality total RNA samples (OD260/280=1.8~2.2, RIN≥6.5) was used for each RNA-seq library. Libraries were size selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. The final sequencing libraries were quantified by TBS-380 Mini-Fluorometer (Promega).