title: GSM8730587: CRISPR-Cas9-medium-1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted with the TaKaRa MiniBEST Universal Genomic DNA Extraction Kit (TaKaRa, Japan). Library sequences were amplified using high-fidelity KOD-Plus-Neo DNA polymerase (TOYOBO, Japan). For each library, the number of PCR cycles has been determined to ensure that the amplification is in the exponential phase. Library construction and high-throughput sequencing were performed by Novogene (China). The PCR products were processed for sequencing library preparation using the NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina (NEB, USA), following the High Inputs (100–500 ng) protocol.