title: GSM8728702: WE U18, Biol rep 2; Saccharomyces cerevisiae; RNA-Seq
library_construction_protocol: Cells were centrifuged and treated with 2U zymolyase for 30 minutes at 37°C. RNA was extracted using the E.Z.N.A. Total RNA Kit I (OMEGA), according to the manufacturer's instructions. Subsequently, the samples were treated with DNase I (Promega) to eliminate genomic DNA contamination. RNA integrity was confirmed using a Fragment Analyzer (Agilent). The samples were then sent to BGI, where the preparation of the libraries and sequencing were carried out employing DNBseqTM technology.