title: scRNAseq of HLA-A*02:01-G5R18-26-specifi CD8+ T cells
design: scRNAseq of HLA-A*02:01-G5R18-26-specifi CD8+ T cells
library_construction_protocol: Convalescent participants underwent a blood sample at least 28 days following their initial presentation and details regarding their clinical background, presentation and management were collected. Vaccinated participants had blood sampling 28 after their second dose of MVA-BN vaccination.  Single cells were directly sorted into 96-well PCR plates (Thermo Fisher Scientific) containing cell lysis buffer and stored at -80°C for further analysis. Reverse-transcription (RT) and PCR amplification were performed as described with the exception of using ISPCR primer with biotin tagged at the 5′ end and increasing the number of cycles to 25. Sequencing libraries were prepared using the Nextera XT Library Preparation Kit