title: Heat stress response in the regenerative flatworm Macrostomum lignano
design: Heat stress response in the regenerative flatworm Macrostomum lignano
library_construction_protocol: Animals were starved for 48 h, changing the sea water medium after first 24 h and right before the experiment.  Total RNA was extracted using TRIzolâ„¢ Reagent (Invitrogen). RNA-seq libraries were prepared using Smart-3SEQ protocol from 100 ng of total input RNA.