title: Illumina MiSeq paired end sequencing
design: V4 region of the 18S rRNA using primers 528iF and 964iR
library_construction_protocol: DNA extracts were prepared for paired-end MiSeq sequencing (Illumina, CA, USA) using a two-step PCR method to amplify the V4 region of the eukaryotic 18S rRNA  genes, and to attach MiSeq adapter and barcode sequences. 