title: GSM8952689: Circulating_C-1; Mus musculus; RNA-Seq
library_construction_protocol: For RNA-seq, total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. For ChIP-seq, chromatin was extracted from paraformaldehyde-fixed cells lysed with SDS lysis buffer, followed by sonication. For ATAC-seq, nuclei were isolated by cell lysis and immediately subjected to transposition. RNA-seq libraries were prepared using the TruSeq RNA Sample Prep Kit v2 (Illumina) following the manufacturer's protocol. ChIP-seq libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina, including adapter ligation and PCR amplification. ATAC-seq libraries were prepared from transposed DNA fragments using PCR amplification (11–12 cycles) prior to sequencing. All libraries were sequenced on an Illumina NextSeq500 system.