title: GSM9220270: Astrocytes Donor A32 mScarlet R4; Homo sapiens; RNA-Seq
library_construction_protocol: (RNA) Total RNA was extracted from human astrocytes using the Qiagen RNeasy kit, following the manufacturer's protocol. Cells were lysed, and RNA was isolated via column-based purification, including on-column DNase treatment to remove genomic DNA contaminants. (RNA) The library preparation was done using the QIAseq Stranded mRNA Kit (QIAGEN). From 1000 ng starting material, the mRNA was enriched. The RNA was heat fragmented. After first and second strand synthesis, the cDNA was end-repaired and 3' adenylated. Sequencing adapters were ligated to the overhangs. Adapted molecules were enriched using by 11 cycles or PCR and purified by a bead based cleanup. ). Library preparation was quality controlled using capillary electrophoresis (Tape D1000).High quality libraries are pooled based in equimolar concentrations.