title: GSM8471497: Male WT CV, PBS + insulin, bio rep 4; Mus musculus; RNA-Seq
library_construction_protocol: After endpoint dissection, tissues were homogenized and preserved in Trizol at -80 °C before RNA extraction. To purify RNA, samples were processed with PureLink™ RNA Mini Kit (Invitrogen, 12183025) according to the manufacturer's instructions, and the RNA concentration and quality in the samples were checked with a spectrophotometer (Thermo Scientific, NanoDrop 2000). After being enriched by oligo dT and fragmented, a cDNA library was generated by reverse-transcribing mRNA using random N6-primed RT.