title: mRNA sequencing of stable CDK13 knockdown cell lines
design: Total RNA from cells with stable knockdown of CDK13 (shCDK13) were isolated using RNA Easy Fast Cell Kit (TIANGEN, DP451-TA) with DNase I (TIANGEN, RT411) treatment according to the manufacturers instructions. Isolated total RNAs were examined by Agilent 2100 bioanalyzer (Agilent Technologies, A, USA) to assess RNA integrity and quantity. mRNA libraries were constructed and sequenced by Novogene Co. Ltd using Fast RNA seq lib prep kit v2 (ABclonal, rk20306). Libraries were examined using Qubit, RT-qPCR and Bioanalyzer. Qualified libraries were sequenced on an Illumina NovaSeq X Plus platform with 2  150 bp paired-end reads in a reverse strand-specific manner.