title: RNA-Seq of bos taurus
design: Muscle and liver samples were collected from Wagyu-Angus crossbred cattle and divided into four groups (n=4 per group) based on intramuscular fat (IMF) content: high liver (HL), high muscle (HM), low liver (LL), and low muscle (LM). Total RNA was extracted using Trizol reagent (Invitrogen). RNA quality was assessed, and only samples with RIN  7.0 were used. RNA-Seq libraries were prepared using the Illumina TruSeq RNA Library Prep Kit and sequenced on the NovaSeq 6000 platform by Shanghai Personal Biotechnology Co., Ltd. Sixteen samples (4 HL, 4 HM, 4 LL, 14 LM) were sequenced to identify differentially expressed genes (DEGs) associated with IMF content.