title: GSM8473195: Whole heart tissue, Con-OFF, rep 1 [Bisulfite-Seq]; Mus musculus; Bisulfite-Seq
library_construction_protocol: Whole hearts were excised and were snap frozen in liquid nitrogen and stored at - 80 °C until subjected to further analysis. DNA was isolated using Qiagen DNeasy DNA isolation and purification kit following the manufacturer's guidelines. DNA was subsequently sent to Zymo Research for bisulfite conversion, library preparation, and reduced representation bisulfite sequencing (RRBS). RRBS was performed via Zymo's Methyl-MidiSeq protocol.