title: GSM8472855: LSK, R396Q, replicate 3-1, scRNA-Seq; Mus musculus; RNA-Seq
library_construction_protocol: To isolate bone marrow (BM) hematopoietic cells in adult mice, tibias, femurs, and pelvic bones were removed and crushed in ice cold PBS, followed by passage through 70μm filters. Cells were then lysed, washed, and resuspended in FACS buffer followed by sorting for LSK cells. Expression libraries were constructed according to the Chromium 10X single cell 3′ reagent v3.1 protocol.