title: sequence of phage genome
design: The required genomic sequencing library was constructed using the standard Illumina TruSeq Nano DNA LT library preparation protocol. DNA fragmentation was performed using Covaris, followed by magnetic bead-based purification and size selection of target fragments. The DNA fragments underwent end repair with End Repair Mix, and an 'A' base was added to the 3' ends to facilitate adapter ligation. After adapter ligation, the ligation products were purified. The DNA library was enriched through amplification, and its fragment size and distribution were validated using the Agilent Bioanalyzer 2100. Finally, the DNA library was progressively diluted and quantified to 4-5 pM for sequencing.