title: ITS amplicon metabrcoding shoe dust
design: For fungal metabarcoding, the ITS1 region was amplified using indexed ITS1catta and non-indexed ITS2 primers. Sequencing was conducted using the PacBio Illumina NovaSeq 6000 platform. The ITS region was amplified from pure fungal cultures using universal primers ITS9mun and ITS4ngs.