title: GSM9145964: skin_C8STINGKO_1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from skin tissue using the RNeasy Plus Mini Kit (74136, Qiagen). Skin was weighted at 30mg, snap frozen in liquid nitrogen and stored at -80°C before lysed. Skin tissues were lysed in lysis buffer (Buffer RLT + 40ul DTT 1M per ml + 0.5% Reagent DX) according to the kit's manufacturer instructions in TissueLyser II (85300, Qiagen) using 7 mm stainless steel beads (69990, Qiagen). Extracted RNA was stored at -80°C. Library preparation was performed using the Lexogen QuantSeq 3' mRNA sequencing protocol.