title: RNA-seq of marine sponge: Pheronema carpenteri 2
design: gDNA added to a 50L PCR reaction mixture containing DreamTaq 2x MasterMix (Fisher Scientific, UK), 1.5L of both 20mM forward and reverse primers of the V3-V4 region 341F (5CCTAYGGGRBGCASCAG3) and 806R (5-GGACTACNNGGGTATCTAAT-3)