title: Rewiring the transcriptome of castration-resistant prostate cancer through CDK9 inhibition dataset 3
design: Rewiring the transcriptome of castration-resistant prostate cancer through CDK9 inhibition dataset 3
library_construction_protocol: Cells were plated in 6-well tissue culture plates at 50% confluence. After 24 h, cells were treated with DMSO or 100 nM PRT2527 for 24 h. Cells were lysed with 500 µl TRIzol Reagent (15596026, Thermo Fisher Scientific)  RNA was extracted using Direct-zol RNA MiniPrep kit (R2052, Zymo Research).  NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences.