title: GSM9477503: PBS Sirpa+ DC Sample 1; Mus musculus; RNA-Seq
library_construction_protocol: Thymic pDC and Sirpa+ DC sorted using BD FACS Aria III. As CD11c low B220+ for pDC, and CD11c high Sirpa+ for Sirpa+ DC. Total RNA of sorted cells was extracted with TRIzol (Invitrogen). For cDNA library construction, Ovation SoLo RNA-Seq Mouse (TECAN) was utilized according to the manufacturer's protocol, with 5 ng of total RNA used per sample.