title: miRNAseq of homo sapiens: blood
design: Total miRNA was extracted using the Qiagen PAXgene Blood miRNA Kit for microRNA isolation (QIAGEN, Germantown, MD) according to the manufacturers instructions. RNA yield, quality, and size were assessed through RNA 6000 Nano Assays using a 2100 Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA). Sequencing libraries were constructed using QIAseq miRNA Library Kit with QIAgen miRNA 96 Index IL (QIAGEN, Valencia, CA) according to the manufacturers protocol.