title: RNA-seq of Brachyelytrum erectum: Red River Gorge population
design: Total RNA was extracted from young shoots of Brachyelytrum erectum using the Qiagen RNeasy Plant Mini Kit. The RNA samples were treated with the Invitrogen DNA-free DNA Removal Kit  to remove contaminatiing DNA. High-quality RNA was then submitted to sequencing facility (Novogene Inc.) for transcriptome sequencing.  Directional mRNA sequencing, following poly(A) selection, was performed on either the Illumina NovaSeq 6000 or NovaSeq X Plus platform to obtain paired-end reads of 150 bp.