title: Full-length 16S rRNA sequencing of haemonchus contortus microbiome
design: 27F-1492R covers V1-V9Raw sequences were initially processed through the PacBio SMRT portal. Sequences were filtered for a minimum of 3 passes, and a minimum predicted accuracy of 90% (minfullpass = 3, minPredictedAccuacy = 0.9). The predicted accuracy of 90%, which is defined as the threshold below which a CCS is considered as noise. The files generated by the PacBio platform were then used for amplicon size trimming to remove sequences outside the expected amplicon size (minLength 1340 bp, maxLength 1656 bp). The reads was assigned to samples based on their unique barcode and truncated by cutting off the barcode and primer sequence.