title: Whole-exome sequencing of human genomic DNA: skin
design: DNA was isolated from formalin-fixed paraffin-embedded tissues using the GeneRead FFPE kit following Qiagen recommendations. Paired-end libraries were prepared with as low as 10 ng of FFPE DNA using the SureSelect XT Low Input Reagent Kit. Libraries were sequenced at an average coverage of approximately 80x following Illumina's standard protocol using the Illumina cBot and HiSeq 3000/4000 PE Cluster Kit. The flow cells were sequenced as 150 X 2 paired end reads on an Illumina HiSeq 4000 using the HiSeq Control Software HD 3.4.0.38 collection software. Base-calling was performed using Illuminas RTA version 2.7.7.