title: GSM8951865: A549, NDV, 18h, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol® Reagent following the manufacturer's protocol. RNA quality was assessed via 5300 Bioanalyser and NanoDrop ND-2000, with only high-quality RNA (OD260/280=1.8~2.2, OD260/230≥2.0, RQN≥6.5, 28S:18S≥1.0, >1μg) used for sequencing library construction. RNA-seq libraries were prepared at Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. using the Illumina® Stranded mRNA Prep, Ligation protocol. PolyA-selected mRNA was fragmented, reverse-transcribed into cDNA, end-repaired, and adapter-ligated, followed by PCR amplification and size selection (300 bp). Libraries were sequenced on the NovaSeq X Plus platform.