title: Temporal and Spatial Dynamics of Antimalarial Drug Resistance in Senegal between 2013-2022.
design: DNA was extracted from dried blood spots (DBS) using QIAmp DNA Blood Mini Kit. PCR amplicons were bead-purified (Ampure) and quantified by Qubit and adjusted to equivalent concentration. Library prep was performed with Illumina DNA prep kit using unique dual indexes (UDIs). After library prep, samples were bead-purified and quantified by Qubit. All samples were normalized to 4nM. After normalization, libraries were pooled, purified, quantified, and sequenced on an Illumina MiSeq instrument.