title: RNA-seq of wheat rhizospher under drought
design: Total RNA was extracted from 2 g of rhizosphere soil using the RNeasy PowerSoil Total RNA Kit (QIAGEN, Canada). Extracted RNA was treated with DNAse (ThermoFisher, Canada) to remove the DNA prior to sequencing. The absence of DNA was confirmed by the lack of PCR amplification using 16S rRNA gene specific primers. Total RNA was sent for two lanes of NovaSeq6000 S4 PE150 sequencing at the Centre dExpertise et de Services Gnome Qubec (Montral, Qubec). Libraries were created using a microbial ribosome subtraction approach.