title: Canine axial osteosarcoma
design: 100 ng of genomic DNA was prepared using the KAPA Hyper Prep Kit with Library Amplification Primer Mix with palindromic forked adaptors containing a unique 8-base index sequence. Libraries (2.2nM each) were pooled and sequenced on a HiSeqX platform using 151 bp paired-end reads. Normal tissue samples were sequenced to a target depth of 30x and tumors to a target depth of 60x.