title: GSM8969659: SR16-005712_A1; Homo sapiens; OTHER
library_construction_protocol: 10x Genomics Visium V2 assays were carried out following 10x Genomics protocol CG000520-Rev B. Human whole transcriptome probe panels were added to the tissue, enabling hybridization and of each probe pair across the transcriptome. Slides were processed through probe ligation and prepared for analyte transfer. Tissue slides and Visium CytAssist Spatial Gene Expression v2 Slides were then loaded into the Visium CytAssist instrument, where they were brought into proximity with one another. Gene expression probes were released from the tissue upon CytAssist Enabled RNA Digestion & Tissue Removal, enabling capture by the spatially barcoded oligonucleotides present on the Visium slide surface. The Visium CytAssist Spatial Gene Expression v2 slides were removed from the Visium CytAssist for downstream library preparation. Barcoded products were cleaved from the slide surface and amplified. Gene expression libraries were generated from each tissue section and sequenced on an Illumina NovaSeq6000.