title: GSM9405471: isl1-cpf1_sib-homeo-1; Danio rerio; RNA-Seq
library_construction_protocol: mcherry+ cells were FACsorted after Trypsin larvae dissociation. For RNA-seq, cells were sorted into Trizol and RNA was extracted using phenol/chloroform extraction method. For ATAC-seq, nuclei were extracted and tagmentation performed as described in Buenrostro, Jason D et al., Nat. Methods (2013) and Corces, M. R. et al., Nat. Methods (2017). RNA libraries for RNA-seq were prepared using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing following manufacturer's protocols. For ATAC-seq, DNA fragments were purified using the Monarch PCR & DNA Cleanup Kit following manufacturer's instructions. Libraries were constructed using Illumina Nextera Dual Indexing as described in Corces, M. R. et al., Nat. Methods (2017).