title: RNA-Seq of homo sapiens: B Cell Memory Repertoire Following CoronaVac Vaccination
design: The sequencing library was prepared from total RNA extracted separately from purified naive and memory B cells using an RNeasy mini kit. Complementary DNA (cDNA) was synthesized using the High-Capacity cDNA Reverse Transcription Kit. The cDNA library was then constructed using standard protocols for high-throughput sequencing, with a focus on capturing the variable regions of immunoglobulin heavy chains (IgG, IgA, IgM) to study the B-cell memory repertoire following CoronaVac vaccination.