title: GSM9004900: D002 free-living mycelium 2; Pisolithus microcarpus; RNA-Seq
library_construction_protocol: Total RNAs was extracted from ECM tissues and FLM of the parental dikaryon (D002) and three selected monokaryons (M057, M091, and M120) using a CTAB and lithium chloride-based method (Chang et al., 1993). We selected these monokaryon strains based on their ability to form ECMs with poplar clones. Total RNA was quantified, and integrity waschecked using an Experion Automated Electrophoresis Station (Bio-Rad, Hercules, CA, USA). Preparation of libraries from total RNA (TruSeq Stranded mRNA Kit Illumina) and 2 x 125 bp Illumina HiSeq2500 sequencing was performed at the GET platform (Gé-nopole Toulouse Midi-Pyrénées, Auzeville, France) following their standard protocol.