title: WGS of Ctr L2: cis-tmeA
design: C. trachomatis with cis-complementation of tmeA was generated using the allelic replacement strategy according to established FRAEM protocols. Transformation of chlamydial was accomplished using CaCl2-mediated chemical transformation and clonal isolates for the final Chlamydia strain was obtained by limiting dilution in 384 plates. Individual isolates were expanded for genomic DNA extraction, using the Macherey-Nagel Nucleospin Tissue Kit. Resulting gDNA was prepared for whole-genome sequencing (WGS) by SeqCenter, LLC (diluting to 20 ng/uL with a total volume of 30uL). Illumina WGS sequencing targeted ca. 106 Mbp total paired-end reads, with the Illumina sequencing methods used available on the SeqCenter LLC website.