title: DXZ296A
design: Genomic DNA was extracted from plant and microbial samples using CTAB lysis buffer (2% -mercaptoethanol) with 65 incubation for 60 minutes. DNA fragmentation was performed via mechanical shearing (target size 350-550 bp), followed by size selection using AMPure XP beads. Libraries were constructed through end repair, A-tailing, and adapter ligation (IDT xGen UDI adapters), with final amplification via 8 PCR cycles. Single-stranded circular DNA was generated by denaturation and splint ligation, followed by rolling circle amplification (RCA) to produce DNA nanoballs (DNBs). Sequencing was performed on the DNBSEQ-T7 platform using combinatorial Probe-Anchor Synthesis (cPAS).?