title: GSM9478637: lnc13-KO CD8 IL15-biol rep 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted using Zymo RNA quickFor whole tissue RNA, samples were extracted and flash frozen in liquid nitrogen. Then, samples are homogenized with mortar and pestle containing small amounts of liquid nitrogen to keep samples frozen. Homogenized samples were then transferred to TRIzol®. TRIzol® RNA extraction was then performed as directed by the product protocol. For extraction of RNA from cells, we utilized Quick-RNA Miniprep Kit (Zymo). NEBNext Ultra II RNA library prep kit for Illumina for tissue, LPL and IEL samples. NEBNext Single Cell/Low input RNA library Prep kit for CD8 samples. NEBNext Ultra II DNA Library Prep Kit for illumina for RAP samples.