title: GSM9222368: Developing_Root_Vascular 3 mM KCl experiment 1; Pinus pinaster; RNA-Seq
library_construction_protocol: All the RNA extractions from the microdissection procedure were carried out using manufacturer's instruction protocol (non‐LCM) for the RNAqueous‐Micro RNA Isolation Kit (Ambion, USA). RNA quality, DNA contamination and first quantification were performed via RNA Pico Assay for the 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). Quantification was verified via a Qubit RNA BR (Broad-Range) Assay Kit (Invitrogen, Paisley, UK).  RNA samples with RNA integrity number (RIN) higher than 7 were used for subsequent RNA sequencing. The low input RNA-seq was carried out by Novogen. The cDNA synthesis and amplification, and the library preparation was made with the SMART-Seq™ v4 Ultra™ Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA) following the manufacturer's instructions. RNA sequencing was made in a NovaSeq 6000 sequencer according to the manufacturer's instructions for paired-end reads (Illumina, San Diego, CA, USA). The 24 samples were sequenced producing paired-end reads of 150 bp length.