title: GSM8768244: ONS76_Static_Rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was collected from static control or orbital shaker-treated ONS76 and DAOY cells cultured in aCSF. Total RNA was collected after 18 hours of treatment using RNeasy Plus Mini Kit (Qiagen, #74134). RNA integrity number (RIN) was determined using Agilent Bioanalyzer. All samples had RIN > 9.8. Library preparation was performed using NEBNext Ultra II Directional polyA mRNA Library Prep Kit. Sequencing was performed on Illumina NovaSeq with 30 million paired end reads per sample at 100 bp read length.