title: BSR-seq of wl1 mutant
design: For RNA extraction, unexpanded leaf tissue from 30 wild-type plants and 30 wl1 mutant plants was separately pooled by genotype, yielding two pools. This procedure was performed for each biological replicate. Total RNA was isolated using TRIzol reagent (Thermo Fisher Scientific) and purified with the RNeasy Mini Kit (Qiagen). RNA sequencing libraries were constructed with the Illumina standard library preparation kit. Two biological replicates were included for both the wild type and the wl1 mutant. RNA sequencing was carried out on an Illumina HiSeq 2500 platform.