title: GSM8974917: J3515_s; Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA was isolated from the 190 µl elution of EV solution with Norgen Biotek's Exosomal RNA Isolation Kit (CAT# 58000), following manufacturer's instructions. Quality and concentration were monitored by the RNA Pico 600 Assay Kit for the Agilent 2100 Bioanalyzer System (5067-1513). Small non-coding libraries were produced by New England BioLabs' NEBNext Multiplex Small RNA Library Prep Set (E7300S and E7580S). Library quality was assessed on samples by the High Sensitivity DNA Assay Kit for the Agilent 2100 Bioanalyzer System (5067-4626). Samples were sequenced by the UPMC Genome Center, Pittsburgh PA, on the NextSeq 2000 machine generating single-end reads. Data was received in .fastq.gz formatting.