title: GSM8473142: erythroid cells, G_ATACseq, D10, rep1; Homo sapiens; ATAC-seq
library_construction_protocol: 10-day differentiated erythroid cells from UCB-CD34+ were electroporated using Lonza P3 primary cell kit (V4XP-3024) and lysed after 48 hours of culture or treatment in lysis buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 8 mM MgCl2 and NP-40) on ice for 5 min to extract nuclei. The cells were centrifuged at 500 g for 5 min to remove the supernatant. The nuclei were incubated with the Tn5 transposome and corresponding buffer at 37°C for 30 min (Vazyme, TD501). The stop buffer was added to the reaction to terminate the tagmentation. DNA Clean Beads were used to purify DNA. A subsequent PCR step was used to add an additional index to the fragments. Two biological replicates per sample were sequenced using a Novaseq 6000 (PE150, Illumina) by Novogene (Beijing).