title: GSM9326836: GM-BMCs treated with cGAMP (5 μg/ml); Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow cells were isolated from the legs of mice and cultured in RPMI 1640 (Nacalai Tesque, Kyoto, Japan) containing 10% foetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO, USA) and 10 ng/ml of GM-CSF (PeproTech, Cranbury, NJ, USA). Red blood cells (RBCs) were lysed with RBC lysis buffer (Thermo Fisher Scientific, Waltham, MA, USA) before culture. After 4 days, additional GM-CSF (10 ng/ml) was added and used for the experiment on days 6–7. GM-BMCs were treated with HCQ (100 μM) or cGAMP (5 μg/ml) for 6 h. Sequencing was performed in paired-end mode (read1: 28 bp; read2: 100 bp) using a DNBSEQ-G400 sequencer (MGI Tech, Shenzhen, China).