title: ddRADseq of Petunia x hybrida: leaf
design: Libraries produced digesting gDNA with PstI and MspI endonucleases, ligating barcodes, dividing into two pools, size selecting at 100-600 bp, amplifying, checking with Qubit fluorometry and Bionalyzer, single-end sequencing in two runs with the Ion GeneStudio S5 Plus System sequencer.