title: RNA Iso-seq of Pyrus communis cv. Bartlett: buds, flowers, leaves, fruit, stems, roots
design: Eleven tissue types collected from P. communis cv. Bartlett throughout 2 seasons: Early (~0.25 inches in size) and late (~0.5 inches) buds, flowers, young and mature leaves, whole immature fruit, and mature fruit divided into peels, cortex, and seeds, were collected from 30 year old trees, and stem and root tissues were collected from micropropagated and rooted plantlets. RNA extracted using modified CTAB protocol. RNAs used as input for PacBio Iso-Seq Express 2.0 kit, size selected with 0.86X beads, and sequenced on PacBio Revio platform.