title: GSM8830322: F1_1; Triticum aestivum; RNA-Seq
library_construction_protocol: Total RNA was extracted using Trizol reagent (thermofisher, 15596018) following the manufacturer's procedure. The total RNA quantity and purity were analysis of Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA, 5067-1511) , high-quality RNA samples with RIN number > 7.0 were used to construct sequencing library. RNA libraries were prepared for sequencing using standard Illumina protocols