title: DNA-Seq of the fungal communities:rhizosphere soil from intercropping buckwheat (Fagopyrum esculentum Moench) and paulownia (Paulownia elongata x Paulownia fortunei)
design: Meta-barcoding analysis of the microbial community was performed based on the variable region of ITS1 for fungi. Specific primer sequences  ITS1FI2 and 5.8S were used to amplify the selected region and prepare the library. PCR was performed using Q5 Hot Start High-Fidelity 2X Master Mix, reaction conditions according to manufacturers recommendations