title: GSM9583525: very young leaf, glabrous, rep 3; Brassica napus; RNA-Seq
library_construction_protocol: Total RNA was extracted from very early third leaves of DH6-47 and DH6-110 using the RNeasy Trizol reagent (Qiagen) following the manufacturer's procedure To generate cDNA libraries for sequencing, 10 mg of total RNA with a RIN quality higher than 7 as assessed using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) was subjected to poly-A mRNA selection using poly-T oligo magnetic beads (Invitrogen). cDNA libraries were prepared by Thermo Fisher Scientific (Waltham, USA) with an average insert size of 300±50 bp for the paired-end libraries