title: PacBio read
design: PacBio sequencing was carried out on a Pacific Biosciences RSII instrument using SMRTbell library preparation. HMW DNA was first sheared to the 5,000 bp fragment size, followed by damage repair and ligation of SMRTbell adapters (Pacific Biosciences, Menlo Park, CA). The resulting libraries were size-selected, purified, and subjected to single-molecule real-time (SMRT) sequencing on the RSII system.