title: RNA-Seq of human: subgingival ant19
design: DNA was extracted using the QIAamp DNA Microbiome kit (QIAGEN, Hilden, German). Library preparation was performed targeting the V3-V4 region of the 16S SSU rRNA gene using primers 515F: GTGYCAGCMGCCGCGGTAA (Parada et al., 2016) and 806R: GGACTACNVGGGTWTCTAAT (Apprill et al., 2015).