title: GSM9443664: qd150_normal tissue; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from clinical samples using RNAiso Plus (Takara Bio, cat. no. 9109) according to the manufacturer's instructions. RNA integrity was assessed by agarose gel electrophoresis, and RNA concentration was measured using a Qubit fluorometer (Thermo Fisher Scientific). RNA-seq libraries were prepared from total RNA according to the standard protocols provided by BGI Genomics (Shenzhen, China). Briefly, mRNA was enriched from total RNA, fragmented, and reverse-transcribed into cDNA. After end repair, adapter ligation, and PCR amplification, the libraries were quality-checked and sequenced using paired-end 2 × 150 bp reads on an Illumina NovaSeq 6000 platform.