title: 16S rRNA amplicon sequencing of Macrobrachium rosenbergii: water microbiota
design: The sequencing library was constructed by PCR amplification of the 16S rRNA gene V3-V69 region using barcoded primers. Following amplification, the PCR products were purified and size-selected via gel extraction. Adapter sequences were then ligated to the target amplicons using the TruSeqTM DNA Sample Prep Kit. The final library was quantified and normalized prior to sequencing.