title: GSM8639734: PBMC, LPVL, B7; Bos taurus; RNA-Seq
library_construction_protocol: Peripheral blood mononuclear cells (PBMCs) were isolated with Ficoll-Paque Plus (GE Healthcare, Uppsala, Sweden) density gradient centrifugation and resuspended in RNAlater® (Ambion, Austin, TX, USA). Total RNA was extracted with High Pure RNA Isolation Kit (Roche). Sequencing libraries were prepared with the TruSeq Stranded mRNA kit (Illumina, San Diego, CA, USA),