title: ITS1 sequencing of DNA
design: Fungal DNA was extracted from maize (Zea mays) tissue samples using the [DNA extraction kit, e.g., DNeasy Plant Mini Kit] according to the manufacturer's protocol. The internal transcribed spacer (ITS1) region of fungal rDNA was amplified using primers ITS1-F and ITS2. PCR products were purified and used to construct sequencing libraries with the [library preparation kit, e.g., NE]()