title: GSM9086503: Resistenz pSCC DMSO rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the NucleoSpin® RNA Plus Kit (Macherey-Nagel, cat. no. 740984.50) following the manufacturer's instructions. RNA concentration was measured using a NanoDrop® ND-1000 spectrophotometer (ThermoFisher). Libraries were prepared using the Illumina® Stranded TruSeq® RNA sample preparation kit, starting with 1 µg total RNA. mRNA was poly-A selected, purified, fragmented, reverse transcribed with random primers, followed by second strand cDNA synthesis. After end repair, A-tailing, and adapter ligation, libraries were purified and amplified by 15 PCR cycles.