title: RNA-seq of Plasmodium berghei PbWT and PbHMGB1KO parasite strains
design: RNA-seq of Plasmodium berghei PbWT and PbHMGB1KO parasite strains
library_construction_protocol: PbWT and PbHMGB1KO parasites were isolated from infected-mouse blood. Total RNA was extracted from PbWT and PbHMGB1KO parasites using RNeasy Mini Kit. mRNAs isolated using magnetic oligo-d(T) beads were used for the first and second strand cDNA synthesis followed by the end preparation of the cDNA libraries and adapter ligations using the manufacturerâ€™s protocols.