title: PRV_RNA_4h_Rep2
design: Total RNA was extracted using the FastPure Cell/Tissue Total RNA Isolation Kit V2 (Vazyme). RNA-seq libraries were constructed from 500 ng of total RNA using the VAHTS Universal V8 RNA-seq Library Prep Kit. mRNA was enriched using poly-A capture beads and fragmented to a size of 300-400 bp. cDNA was synthesized, adapter-ligated, and PCR amplified. The final libraries were purified using KAPA Pure Beads. Library quality was assessed using an Agilent 2100 Bioanalyzer, and sequencing was performed on the Illumina NovaSeq 6000 platform with 150 bp paired-end reads.