title: GSM9326718: WY6; Mus musculus; RNA-Seq
library_construction_protocol: Organoids were harvested in RLT solution, and RNA was extracted using the RNeasy Qiagen kit following the manufacturer's protocol (Cat. No. 74104). The library was prepared by Novogene following their standard pipeline. Briefly, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library.