title: MIC_Rib1-WT
design: A total of five million micronuclei (MICs) were purified from RIB1 knock out Tetrahymena meiotic cells that expressing exogenous Rib1-FL-HA, then the MICs were used for the Cleavage Under Targets and Tagmentation (CUT&Tag) experiment for the identification of Rib1-FL binding regions in the meiotic MIC. CUT&Tag was conducted using BioMag Plus Concanavalin A beads (Bangs Laboratories, Fishers, IN), a commercial Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme, Nanjing, China), and an anti-HA rabbit monoclonal antibody (Clone C29F4, Cell Signaling Technology).  CUT&Tag libraries were generated using indexed primers and the KAPA HiFi HotStart Real-Time Library Amplification Kit (Roche) and subsequently sequenced on a NovaSeq 6000 platform (Illumina, San Diego, CA).