title: GSM9478750: Non-fluorescent late L3-stage cells other than DTC, Sample 2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: The miRNeasy Mini Kit (Qiagen, Cat no. 217004) was used for RNA extraction. We obtained approximately 5-9 ng/μl of RNA from 30,000 to 60, 000 cells. One ng of cDNA was used as input for preparation of sequencing libraries using the Nextera XT DNA Sequencing kit (Illumina). Libraries were prepared according to the manufacturer's instructions.