title: GSM9217477: Vibrio cholerae A1552 single cell rna seq, second run; Vibrio cholerae; RNA-Seq
library_construction_protocol: Intracellular Vibrio cholerae bacteria recovered from Tetrahymena pyriformis and from EFVs were fixed in 4% formaldehyde at 4 degc overnight and then the MicroSPLiT protocol was perfromed to individalluy tage the mRNA of the bacteria Single cell RNA-seq libraries were prepared using Illumina TruSeq Stranded mRNA kit according to the manufacturer's protocol (Illumina, San Diego, CA, USA). Single cell RNA-Seq, The sequencing library were sequenced twice (first and second run) to obtain suffcient cell sumbers across conditions.