title: ATAC_5_48
design: single nuclei suspended in diluted nuclei buffer (10x Genomics) were loaded on a Chromium Single Cell Instrument (10x Genomics) following instructions for the Chromium NextGEM ATAC-seq v1.1 assay. ATAC-seq libraries were prepared using Chromium NextGEM Single Cell ATAC-seq v1.1 Kit (10x Genomics).  Paired-end sequencing was performed on Illumina NextSeq500 (Read 1: 50-bp; 8-bp i7 sample index; and Read 2: 50-bp) Data pre-processing was performed using Cell Ranger ATAC v1.0 and the mouse GRCm38 genome/reference for alignment.