title: ITS.F.4
design: For total soil DNA extraction, the DNeasy PowerSoil Kit (QIAGEN, Inc., Netherlands) was used according to the manufacturer's protocol. To assess the bacterial fraction, the V3-V4 variable region of the 16S rRNA gene was targeted using the primers Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACHVGGGTATCTAATCC). For the fungal fraction, the ITS2 region was amplified using the primers ITS3 ('5-GCATCGATGAAGAACGCAGC-3') and ITS4 ('5-TCCTCCGCTTATTGATATGC-3'). Sequencing was performed using a paired-end 2x250 system on the NovaSeq platform (Illumina, USA) by Novogene Inc. (www.novogene.com).