title: GSM8473190: Sheep I, Pre-term ctrl, I1; Ovis aries; RNA-Seq
library_construction_protocol: Flash frozen tissue was procured from ovine brain at the time of necropsy and sent to GeneWiz for RNA extraction and RNA-sequencing. Total RNA was extracted from frozen cell pellet and fresh frozen tissue samples using Qiagen RNAeasy Plus Universal mini kit following Manufacturer's instructions (Qiagen, Hilden, Germany). GeneWiz conducted RNA-seq library preparation as follows: A) mRNA enrichment, mRNA fragmentation, and random priming; B) first and second strand cDNA synthesis; C) end repair, 5' phosphorylation, and dA-Tailing; D) adaptor ligation, PCR enrichment, and sequencing.