title: GSM9503278: ID8, WT-6; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol and the quality and quantity of total RNA were evaluated using an Agilent 2100 Bioanalyzer. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.