title: 16S rRNA gene sequencing of Mus musculus: wild-type 21d post-FMT replicate 2
design: Fecal microbiota transplantation (FMT) was performed by administering filtered fecal suspensions from donor mice to antibiotic-pretreated recipients. Mice received a broad-spectrum antibiotic cocktail (vancomycin, neomycin, ampicillin, and metronidazole) for 4 weeks prior to transplantation, followed by oral gavage of fecal material. Fecal samples were collected at defined time points during microbiota reconstitution, immediately frozen, and stored at 80 C. Microbial DNA was extracted using the PureLink Microbiome DNA Purification Kit, and the 16S rRNA gene was sequenced using paired-end reads on an Illumina NovaSeq 6000 platform.