title: iGFP_2 (CRX1129668; CRA017616)
library_construction_protocol: To digest the RNA other than RPFs, cell or tissue lysate was treated with unspecific endoribonuclease RNase I. Isolation of monosomes was performed by size-exclusion chromatography with MicroSpin S-400 HR columns. Following PAGE purification, the both ends of RPF were phosphorylated and ligated with 5' and 3' adapters respectively. The RNA samples were then treated with rRNA depletion kit(Qiagen) to deplete the samples of as much rRNA contamination as possible before PAGE purification of the relatively short (20~38 nt) RPFs. Then the fragments were reversely transcribed to the cDNAs and amplified by PCR. submitted_library_strategy: Ribo-seq