title: Transcriptome Sequencing Library of Mouse Submandibular gland Tissue for Drug - Response Study
design: Total RNA was extracted from Submandibular gland of house mouse and its purity, concentration, and integrity were detected. mRNA was enriched using Oligo (dT) magnetic beads for eukaryotes. The mRNA was fragmented and then used to synthesize first - strand and second - strand cDNA. After end - repair, A - tail addition, adapter ligation, and size selection, the library was prepared. Sequencing was performed on the Illumina platform with 150 bp paired - end reads