title: GSM9445037: Sublibrary_5 from multiplexed Polypterus fin samples; Polypterus senegalus; RNA-Seq
library_construction_protocol: Nuclei were fixed and frozen using the Evercode Nuclei Fixation Kit v3 (#NF100, #NF200) from Parse Biosciences following the manufacturer's protocol. snRNA-seq libraries were generated using Parse Biosciences combinatorial barcoding technology.  Fixed and frozen nuclei from each sample were processed to generate barcoded libraries aiming for 7,000 nuclei per sample using the Evercode WT v3 Kit (#WT100A-D, #WT200) as per manufacturer's instructions. Samples were multiplexed during barcoding and sequenced as multiple technical sublibraries at the depth of 20,000 reads per nuclei in an Illumina Novaseq Xplus platform (paired-end, 150bp).