title: YTHDF2ko
design: Total RNA was extracted from human A549 lung cancer cell line using a standard RNA isolation protocol. Small RNA fragments (1830 nt) were size-selected and purified from total RNA. miRNA sequencing libraries were constructed using a DNBseq-based small RNA library preparation kit. Briefly, 3 and 5 adapters were sequentially ligated to small RNAs, followed by reverse transcription and PCR amplification. The final library was quantified and quality-controlled before sequencing on a DNBseq platform to generate single-end small RNA reads.