title: GSM8491207: CD3e+CD8+ T cells in Py8119 tumors from WT mice, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA were extracted using RNAeasy micro-prep kit from Qiagen The integrity of total RNA samples were assessed on Bioanalyzer 2100 using RNA 6000 Pico chip (Agilent Technologies, CA).  Messenger RNA was enriched from these samples using QIAseq FastSelect - rRNA HMR Kit (Qiagen, CA), fragmented and converted to cDNA and Illumina sequencing library using the PrepX RNA-seq library preparation protocol on the Apollo 324TM NGS Library Prep System (Takara Bio, CA).