title: GSM9504840: TM54_shRNA_Control-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA extracted  via RNeasy mini kit (Qiagen #74104) The NEBNext Ultra II RNA Library Prep Kit for Illumina & NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) was utilized according to the manufacturer's recommendations. ERCC RNA Spike-In Mix (ThermoFisher, #4456740) was added to normalized total RNA prior to library preparation following manufacturer's protocol.