title: GSM8975324: HEK293T_Oxsr1 KO_2_R1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from mouse livers was isolated using TRIzol™ Plus RNA Purification Kit (Cat# 12183555, Thermo Fisher) and DNA was removed by treatment with TURBO DNA-free™ Kit (Cat# AM1907, Thermo Fisher). Total RNA was extracted using a standard TRIzol-based protocol. RNA integrity was assessed using an Agilent Bioanalyzer 2100. Libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit according to the manufacturer's instructions, which includes poly(A) mRNA enrichment, fragmentation, cDNA synthesis, adapter ligation, and PCR amplification. Libraries were quantified and quality-controlled before sequencing.