title: GSM9223176: HEK293T cells, IP M4 1; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were harvested and washed with DPBS buffer. Total RNA was extracted using TRIzol reagent (Thermo Scientific) and subsequently purified with Direct-zol RNA MiniPrep (Zymo Research). mRNA was enriched by two rounds of poly(A) purification using oligo(dT)25 magnetic beads (New England Biolabs). RNA concentrations were measured by Nanodrop. RNA-Seq libraries were prepared using NEBNext Ultra II RNA Library Prep Kit (New England Biolabs).