title: Rhizosphere soil from mock-treated *Brassica oleracea*, ITS sequencing: low aphid density experiment
design: Rhizosphere soil DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen). The ITS2 region was amplified using primers ITS1f (5-CTTGGTCATTTAGAGGAAGTAA-3) and ITS2 (5-GCTGCGTTCTTCATCGATGC-3). Amplicon libraries were sequenced at a minimum depth of 100,000 paired-end reads (2  250 bp) per sample on the Illumina NovaSeq 6000 platform by Genome Quebec.