title: Full 16S rRNA for anerobic microbiome
design: Library preparation followed the Oxford Nanopore 16S V14 kit protocol, incorporating dual barcodes per sample, and sequencing was conducted on a Nanopore flow cell (R10.4.1). Basecalling was performed using Dorado (v7.3.9) with a minimum Q score of 10, a minimum read length of 200 bp, and a basecalling speed of 400 bp/s.