title: Soil microbial community
design: Soil DNA was extracted with Revio SPRQ chemistry. Full-length 16S rRNA amplicons were generated using SuperFi II PCR enzyme, pooled, and purified. Libraries were prepared with PacBio Kinnex kits and sequenced on a PacBio Revio using a 30-hour run. Data were processed with skera, lima, and PacBio HiFi-16S workflow for ASV generation and taxonomic classification