title: 16S rRNA of Microbial enrichement from sample BC13 under anaerobic and 30 degree celsius condition supplemented with sphalerite
design: Library construction is carried out following the standard protocol of the ALFA-SEQ DNA Library Prep Kit, and the size of the library fragments is evaluated on the Qsep400 High-Throughput Nucleic Acid & Protein Analysis System (Hangzhou Houze Biotechnology Co., Ltd., China). The concentration of the library is measured using a Qubit4.30 (Thermo Fisher Scientific, Waltham, USA).