title: GSM9329292: CB102_MLL_11_minusAF4_d79; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel, 740955.50) according to the manufacturer's instructions. Library preparation and sequencing was performed at GenXPro GmbH (Frankfurt/Main). MACE-seq was used to prepare 3'RNA sequencing libraries. The DNA-depleted RNA molecules underwent the library preparation, using the MACE-Seq Kit (GenXPro GmbH) according to the manual of the manufacturer. Fragmented RNA was reverse transcribed, followed by the template switching combined with the incorporation of the TrueQuant UMI. The cDNA fragments were PCR amplified and purified. Sequencing was performed on an Illumina NextSeq instrument with 1×76 bps.