title: PRO-seq for Pol II occupancy project
design: PRO-seq for Pol II occupancy project
library_construction_protocol: Mouse ES cells were cultured on 0.2% gelatin-coated plates in ES medium (DMEM, supplemented with 15% FBS, LIF, 2-Mercaptoethanol, 2 mM L-Glutamine and 1x non-essential amino acids) at 37C and 5% CO2. Medium was changed daily and cells were split every second day. Precision run-on sequencing (PRO-seq) was performed as previously described (Judd et al., 2020; Kwak et al., 2013) with slight modifications. For the Drosophila S2 cell samples, 10 million Drosophila S2 cells were used per sample with a spike-in of 0.1 million (1%) DNMT TKO mESCs. For the DNMT TKO mESC samples, 5 million DNMT TKO mESCs were used per sample with a spike-in of 0.05 million (1%) Drosophila S2 cells.