title: rice phyllsophere microbiome
design: amplified the V3-V4 hypervariable region of the 16S rRNA using standard Illumina primers (forward 5-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3; reverse 5-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTACHVGGGTATCTAATCC)