title: Total RNA from prometaphase HeLa cells
design: Total RNA from prometaphase HeLa cells
library_construction_protocol: HeLa cells were grown in normal conditions (DMEM high glucose medium, 10% FBS, 37Â°C, 5% CO2). 3 biological replicates were subjected to synchronization to prometaphse using double thymidine block and nocodazol treatment.  RNA extraction was performed using acidic phenol-chloroform-IAA mix and ethanol precipitation. The pellet was resuspended in nuclease free water. Library preparation occured at the DKFZ Core Facility using standard non-stranded library preparation after depletion of ribosomal RNA (Illumina RiboZero protocol).