title: PBMC RNAseq of Linezolid treated TB patients
design: Total RNA was extracted from PBMCs using the PAXgene Blood RNA Kit (TIANGEN, Beijing, China) following the manufacturers instructions. mRNA libraries were prepared using the Optimal Dual-mode mRNA Library Prep Kit (BGI, Shenzhen, China). Briefly, mRNA was enriched using oligo(dT) magnetic beads, fragmented, and reverse-transcribed into cDNA. After end repair, A-tailing, and adaptor ligation, libraries were PCR-amplified and quality-checked. Circularized single-stranded DNA libraries were generated and amplified by rolling circle amplification to form DNA nanoballs (DNBs), which were sequenced using the BGI platform with 150 bp paired-end reads.