title: ITS Metagenome Sequencing of Integrated Nutrient Management R3 - Soil Sample
design: Fungal internal transcribed spacer (ITS) regions were amplified from soil metagenomic DNA using universal primer sets targeting the ITS1ITS4 region of fungal rDNA. Amplicons were purified and subsequently prepared for long-read sequencing using the Oxford Nanopore Technologies Native Barcoding Kit following the manufacturers protocol. Barcoded libraries were pooled and sequenced on the Oxford Nanopore platform to generate high-quality reads for fungal community profiling. This dataset represents amplicon-based fungal metagenomic sequencing aimed at assessing fungal diversity from soil samples.