title: GSM8950875: CPMV treatment 24h Q7V4-3; Homo sapiens; RNA-Seq
library_construction_protocol: The isolation of single cells, purification, library preparation, and sequencing were performed at NCI Sequencing Facility. The single cell RNA-seq libraries were prepared using a Chromium Next GEM Single Cell 5' reagent kit v2 dual index protocol according to the manufacturer's instructions. The libraries were pooled and sequenced on a NovaSeq X Plus flowcell (Illumina) using 10 cycles for dual indices, 26 cycles for Read1, and 90 cycles for Read2 non-symmetric run according to the manufacturer's instructions.