title: DNA sequencing of population G4 at generation 10000
design: We evolved 10 fission yeast populations at 30C in wells of a lidded, flat-bottom 96-well plate containing YPD for 10,000 generations. We chose to perform whole-population whole-genome sequencing every 1,000 generations, and we were able to grow back cells from 93 out of these 100 samples after storage and sequenced these time points with Illumina NovaSeq paired-end sequencing (mean effective coverage ~40X).