title: RNA-Seq of Arabidopsis thaliana; root (WT) under 3-day iron sufficiency
design: Total RNA was extracted from Arabidopsis thaliana root tissues using TRIzol reagent (Invitrogen); mRNA was enriched with oligo(dT) magnetic beads and reverse-transcribed into cDNA; Illumina RNA-seq libraries were constructed using the NEBNext Ultra RNA Library Prep Kit; libraries were quantified by qPCR and sequenced on an Illumina NextSeq 500 platform to generate 150bp paired-end reads.