title: 16S rRNA sequencing of Homo sapiens: fecal sample from healthy elderly control (EldGrp)
design: Fecal samples were collected from elderly individuals and grouped into healthy control, hypertensive without renal impairment, and hypertensive with chronic kidney disease. Microbial genomic DNA was extracted using a commercial stool DNA extraction kit. The V3V4 hypervariable regions of the bacterial 16S rRNA gene were amplified using primers 341F and 806R. Amplicons were purified, quantified, and pooled in equimolar amounts before sequencing. Sequencing was performed on an Illumina NovaSeq 6000 platform using paired-end 250 bp read