title: Sex drives colonic mucin sialylation in wild mice - RNAseq
design: Sex drives colonic mucin sialylation in wild mice - RNAseq
library_construction_protocol: Following excision, proximal colonic tissue was stored in 1mL of phenol reagent, and immediately frozen with dry ice before being stored at -80˚ C Total RNA was extracted using standard precipitation protocols using chloroform and isopropanol and was submitted to the University of Manchester Genomic Technologies Core Facility (GTCF). Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol