title: H3K4me3Q5ser ChIP-seq of H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells
design: H3K4me3Q5ser ChIP-seq of H3.3 or H3.3Q5A expressing PANC-1 cells and TGM2 knockdown PANC-1 cells
library_construction_protocol: The PANC-1 cells were maintained on DMEM supplemented with 10% FBS, 1% penicillin-streptomycin.Collect cells by digesting with trypsin The cells were fixed with 1% formaldehyde and sonicated for 5 minutes on 4 â„ƒ. Next, chromatin fragments were immunoprecipitated with Protein A/G Magnetic beads coupled with antibodies. The eluates were digested with  RNase A and proteinase K. DNA fragments were recovered using the QIAquick PCR purification kit and used for ChIP-seq library construction. ChIP-seq libraries were constructed using NEBNext Ultra II DNA Library Prep kit