title: GSM8800805: darkX14a; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol (Thermo Fisher, USA) according to the manufacturer's instructions. The extraction process included the use of molecular-grade chloroform and isopropanol, and the RNA was subsequently washed with molecular-grade ethanol (all reagents were from Sigma-Aldrich, USA). Subsequent analysis used high-quality RNA (280/260 and 260/230 indexes > 1.8). RNASeq Libraries were prepared using a QuantSeq 3' mRNA-Seq Library Prep Kit-FWD (Lexogen, Vienna, Austria) with 1μg RNA. The library concentration was measured by Qubit Fluorometer and Qubit dsDNA HS Assay Kit (Applied Biosystems), and the size distribution was determined using an Agilent D1000 ScreenTape System (Agilent Technologies).