title: feacel sample
design: The draft genome sequence analysis of ZX030 strain was carried out usingthe Illumina sequencing platform (MajorBio Co., Shanghai, China). Briefly, DNA samples were sheared into ~400 bp fragments using a Covaris M220 Focused Acoustic Shearer following manufactures protocol. Illumina sequencing libraries were prepared from the sheared fragments usingthe NEXTFLEX Rapid DNA-Seq Kit. 5primer ends were first end-repaired and phosphorylated. Next ,Genome assembly and annotation the 3 ends were A-tailed and ligated to sequencing adapters. The third step is to enrich the adapters-ligated products using PCR. The prepared libraries then were used for paired-end Illumina sequencing (2  150 bp) on Illumina Novaseq 6000 (or other new sequenator) .