title: 16S_seq_Aranjuez_Experimental_Station_288
design: Soil DNA was extracted from 1 g of defrosted soil samples using the Canvax Soil DNA Isolation Kit (Canvax Biotech, S.L., Crdoba, Spain). For bacterial communities, the V3-V4 region of the 16S rRNA gene was amplified using 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC) (Ihrmark et al., 2012). For fungal communities, the ITS2 region was amplified with primers fITS7 (GTGARTCATCGAATCTTTG) and ITS4 (TCCTCCGCTTATTGATATGC). DNA extracts (PCR parameters detailed in Table S6) were sequenced on an Illumina MiSeq platform (Caporaso et al., 2012) at the Next Generation Genome Sequencing Facility at Western Sydney University (NSW, Australia).