title: SHAPE MAP VEROE6_Delta NAI1
design: 3 g of viral RNA was retrotranscribed into cDNA using SuperScript II (Invitrogen) following a published protocol. The resulting dsDNA was purified using NGS NucleoMag Beads (Macherey-Nagel, Ref: 744970.50) with a 1:1 bead-to-sample ratio and eluted in 15 L of RNase-free water. Libraries were prepared using End Prep library kit (NEB, #E7546) followed by NEBNext Ultra II Ligation Module kit (NEB, #E7595) with the adapters obtained from NEBNext Multiplex Oligos for Illumina (NEB, #E7600) following standard protocols.