title: WGS of Pseudomonas aerugionsa strain PS23
design: Before preparing the library, DNA quantification was performed using the Qubit 3.0 Fluorometer, Invitrogen. The library preparation was conducted following Illuminas Nextera XT DNA Library Prep kit (Reference Guide 15027987). The specimens were fragmented using a Nextera XT Library Prep Kit to tagment gDNA, which is a process that fragments DNA and then tags the DNA with adapter sequences in a single step. In addition, Illumina's Nextera XT (Set A, B, C, D) dual indexes were applied to allocate distinct tags to each sample, simplifying the process of combining libraries for sequencing. The indexed library was amplified and clean up by 0.6X AmPure bead (AMPure XP). The size of the library was obtained greater than 300bp after clean up. The quantification of completed libraries was performed using Invitrogens Qubit 1X dsDNA HS Assay kit. The average size of the libraries was measured using either a Bioanalyzer 2100, manufactured by Agilent Technologies. The library concentrations were adjusted to a standardised concentration of 4nM and confirmed using Invitrogens Qubit 1X dsDNA HS Assay kit, and Illuminas PhiX control library was used as a reference. The libraries were combined at equal concentrations in groups of 5 samples and final pool was denatured. The final loading concentration of 12pM library was obtained and run on Illumina MiSeq platform using MiSeq V3 kit with a read length of 251 paired-end.