title: Pollen metabarcoding data collected from bumble bee legs (ITS)
design: DNA was extracted from the pollen using a Quick Mini-prep DNA/RNA extraction kit (Zymo Research, Irvine, CA). A three-step nested PCR design was used for amplification, with the ITS1 and ITS2 regions amplified using universal plant primers in the first PCR, the next generation sequencing read-priming oligonucleotides added in the second PCR, and dual multiplex indices to label each sample added in the third PCR.