title: DAP-seq of Solanum lycopersicum: SlWO-rep2
design: Genomic DNA was extracted from leaves tomato Moneymaker cultivar and used to construct the DNA library. The CDS of candidate TFs were cloned into the pH6HTN His6HaloTag T7 vector and the HALO-tagged TFs were expressed in vitro using the wheat germ protein expression system. The expressed HALO-TF proteins were bound to magne HaloTag beads and were used for binding to DNA, followed by PCR enrichment, indexing and pooling of individual libraries for sequencing.