title: Apple calli
design: Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA wassynthesized using random hexamer primers followed by the secondstrand cDNA synthesis. The library was ready after end repair, A-tailing,adapter ligation, size selection, amplification, and purification. Thelibrary was checked with Qubit and real-time PCR for quantification andbioanalyzer for size distribution detection.