title: H3K4Me3
design: Cells were suspended in PBS and formaldehyde used to cross-link chromatin, which was isolated after lysis with Triton X-100 and sonication. The sheared chromatin incubated with antibodies against specific histone modifications and immunoprecipated using Protein G Dynabeads. After incubation with Proteinase K, DNA was isolated using phenol:chloroform extraction.  Sequencing libraries were prepared using the NEBNext Ultra II Library Preparation protocol.