title: Endophyte free Ryegrass plant with no GA3 applied
design: Bacterial communities associated with soil samples were characterised by sequencing the V5/V7 region within the bacterial 16S ribosomal RNA (rRNA) gene. with an Illumina MiSeq instrument (paired end, 2  250bp) (Illumina, USA). Sequencing was performed on the 48 soil samples from the two copies of the same 24 plant genotypes selected for transcriptome analysis. The DNA extraction was performed on 500 mg of fresh soil samples using the FastDNA Spin Kit for Soil (MP Biomedicals, USA). DNA purity and concentration were determined with NanoPhotometer spectrophotometer (IMPLEN, USA). Libraries for sequencing were prepared by Macrogen Inc. (Korea) using the primers 16S-799F: AACMGGATTAGATACCCKG/16S-1193R: ACGTCATCCCCACCTTCC.