title: Rabbit-H3K4me3-0dpi_Replicate 2
design: The ear pinna tissues from six adult animals were collected for each replicate. The ChIP-seq was carried out as previously described with modifications (Wang et al. 2020; Wang et al. 2023). Briefly, all freshly collected samples used for ChIP-seq were fixed with 1% methanol-free Formaldehyde (Pierce, 28906) in PBS for 12 minutes at RT, followed by the quenching of crosslinking with the addition of glycine at a final concentration of 0.125M. The cells were resuspended in SDS lysis buffer (Beyotime, P0013G) with cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche, 11873580001) and chilled on ice for 30 minutes. The released chromatins were then sonicated into 200-500 bp fragments with Biorupter Pico (Diagenode) at 4 . For immunoprecipitation, the anti-H3K27ac (Abcam,ab4729 for mice; Active Motif, 39685 for rabbits) and anti-H3K4me3 (Abcam, ab8580 for mice; Active Motif, 61379 for rabbits) antibodies were incubated with Protein A/G Magnetic Beads (Pierce, 88803) and diluted chromatins at 4  overnight. The ChIP DNA libraries were made using VAHTS Universal Pro DNA Library Prep Kit for Illumina (Vazyme, ND608). The 150 bp pair-end sequencing was performed for all libraries.