title: GSM9407182: HBV-infected HLCZ01 cells, repeat_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol (Invitrogen), and 1μg total RNA was used for the construction of sequencing libraries. 3 μg RNA per sample was used as the initial material for the RNA sample preparations. Ribosomal RNA was removed using the Epicentre Ribo-Zero Gold Kits (Epicentre, USA). Subsequently, the sequencing libraries were generated following manufacturer recommendations with varied index label by NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, Ispawich, USA). RNA concentration of the library was measured using Qubit RNA Assay Kit in Qubit 2.0 to quantify and then dilute to 1 ng/μl. Insert size was assessed using the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA), and qualified insert size was accurately quantified using Taqman fluorescence probe of AB Step One Plus Real-Time PCR system. Clustering was generated by cBot cluster generation system and then was sequenced on an Illumina platform.