title: GSM8491166: daf-2 mutant, Day 1, Rep 3; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: For each condition and timepoint, a minimum of 80 worms were collected in triplicate. Worms were washed twice with M9, resuspended in 100 µl Tri Reagent (Zymo Research, Cat. No.: R2050-1-200), and then immediately frozen in liquid nitrogen. RNA was extracted with the Direct-zolTM RNA MiniPrep kit (Zymo Research, Cat. No.: R2052) according to the manufacturer's instructions, and RNA amount and quality were determined using a NanoDrop. mRNA was isolated and cDNA constructed by following the TruSeq RNA Library Preparation Kit v2 protocol (Illumina, Cat. No.: RS-122-2001/RS-122-2002). mRNA-seq libraries were constructed using the TruSeq RNA Library Preparation Kit v2 (Illumina, Cat. No.: RS-122-2001/RS-122-2002) according to the manufacturer's instructions.