title: Weaponizing CRISPR/Cas9 for selective elimination of cells with an aberrant genome
design: Weaponizing CRISPR/Cas9 for selective elimination of cells with an aberrant genome
library_construction_protocol: HeLa GFP cells were generated by infecting HeLa WT cells with the pLenti-CMV-MCS-GFP-SV-puro plasmid (#73582, Addgene), previously depleted of the promoter and enhancer regions to generate a stable cell line that does not express the GFP protein. HeLa GFP cells were grown under standard tissue culture conditions (37°C, 5% CO2) in DMEM, supplemented with 10% FBS, 2mM L-Glutamine and 1% Penicillin/Streptomycin. Maxwell® RSC simplyRNA Tissue Kit (Promega) was used to isolate total RNA from cells, according to the manufacturer's instructions. DNase I (Qiagen) treatment was performed to remove any potential residual contamination of genomic DNA.  Illumina stranded mRNA prep Ligation kit for polyA transcripts