title: RNA-seq of keratinocytes with siRNA against GNA13.
design: QuantSeq mRNA-seq V2 (LEXOGEN SKU: 191.96) library prep, using the UMI (Lexogen SKU: 081.96), of primary kerationocytes. 2.5μL of 100µM each siRNA was combined with 25 μL Lipofectamine RNAiMax (ThermoFisher, 13778150) in Opti-MEM (ThermoFisher, 31985062) and incubated for 25 minutes, then the mixture was added to 1 million keratinocytes on a 10 cm dish. Cells were treated with the siRNA-lipid complex for 24-48 hours. Cells were seeded for differentiation or further progenitor growth after 48 h transfection. The negative control was the ON-TARGET Smart pool negative control (Horizon, D-001810-10-20). Total RNA was extracted using the RNeasy Plus kit (QIAGEN, 74136).