title: GSM9121139: rtf1del , ref2del , swd2del , KY5334 , Rep2; Saccharomyces cerevisiae; OTHER
library_construction_protocol: Total RNA was extracted using hot acid phenol extraction method. From 50 µg total RNA input, 4tU-labeled RNA was biotinylated with10 µg/mL MTSEA Biotin-XX (Biotium, 90066) for 30 minutes. The biotinylated RNA was purified and bound to magnetic MyOne Streptavidin C1 beads (Invitrogen, 65001) for 15 minutes. The RNA was eluted off the beads and concenrated using RNeasy MinElute cleanup kit (Qiagen, 74204). 100 ng of RNA was used for library construction. Libraries were prepared using Universal Plus Total RNA-seq with NuQuant kit (Tecan, 0361-A01), UPlus Total RNA AnyDeplete module (Tecan, 0370-A01) and UPlus Total AnyDeplete Custom Probe Mix for S. cerevisiae (Tecan, S02714).