title: GSM9477615: AdV-C5 wt-infected 12-h sample replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were washed with PBS, resuspended in Trizol, incubated at RT for 15min and centrifuged at 16000xg for 30 sec. Supernatant was mixed with equal volume of EtOH and RNA was isolated with Zymo Research Quick-RNA miniprep plus kit according to manufacturer's protocol RNA was prepared for sequencing using the Illumina TruSeq Stranded Total RNA kit according to the manufacturer's protocol. Ribosomal RNA depletion was done with Ribo-Zero rRNA removal kit