title: GSM9661763: P2024_082 Ileum,  rep1; Homo sapiens; RNA-Seq
library_construction_protocol: Biopsies samples were stored in RNAlater™ solution. They were first mechanically dissociated to obtain a single-cell suspension, then processed for total RNA extraction using the RNeasy® Kit (Qiagen), following the manufacturer's protocol. Each RNA sample was eluted in 20μl elution buffer and stored in low-binding tubes. The quality control of the extracted RNA was performed using micro-electrophoresis on the 4200 Tapestation system (Agilent®). RNA samples were stored at a temperature below −80°C. RNA-seq libraries were constructed from a standardized input of 250 ng of total RNA per sample. Libraries were prepared according to the manufacturer's instructions for the Takara SMARTer Stranded Total RNA Sample Prep Kit (634876, Takara Bio USA). Quality control of the RNA-seq libraries was performed using micro-electrophoresis on the 4200 Tapestation system (Agilent®). RNA-seq data were generated using an Illumina NovaSeq X with a 200 bp paired-end read format, targeting 50 million reads per sample.