title: Fungal ITS2 amplicon sequencing of soil metagenome
design: Soil DNA was extracted from 0.2 g of soil using the QIAGEN DNeasy PowerSoil Pro Kit following the manufacturers protocol. Target regions (fungal ITS2 or bacterial 16S rRNA gene) were PCR-amplified for amplicon metabarcoding, indexed, pooled, and sequenced as paired-end reads on an Illumina NextSeq 2000 platform.