title: GSM8472675: triticum_h2o_180_3; Triticum aestivum; RNA-Seq
library_construction_protocol: For RNA extraction, plant samples were ground to a fine powder in liquid N2 and TRIsure™ (BIOLINE, Memphis, TN, USA) was used following manufacturer's instructions. RNA purification was carried out using ISOLATE II RNA Plant Kit (BIOLINE) and RNA integrity was assessed with a Bioanalyzer Agilent 2100 system. Illumina's instructions were followed for mRNA libraries preparation and sequencing was performed on a NextSeq500 Illumina sequencer (Illumina, San Diego, CA, USA)