title: Probiotic Leuconostoc mesenteroides Isolated from Fermented Kodo millet
design: Genomic DNA was extracted from bacterial cultures using the QIAamp DNA Mini Kit, and its quality was assessed via NanoDrop and agarose gel electrophoresis. The 16S rRNA region was amplified using PCR for microbial identification through Sanger sequencing. The purified amplicons were sequenced using specific primers. For shotgun sequencing, high-quality DNA samples were fragmented to 350 bp using Covaris M220 and prepared with the Illumina TruSeq Nano DNA Library Prep Kit. The libraries were analyzed using the Agilent 4200 Tape Station and sequenced on NovaSeq X Plus with paired-end sequencing, ensuring high-throughput data generation.