title: GSM9086899: 22Rv1_BL01D1_R01; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from each sample was extracted using  TRIzol Reagent (Invitrogen). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.