title: GSM9621735: WD 8.5 weeks liver T2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using Qiagen Rneasy Plus Mini kit following manufacturer's instructions (Qiagen, Hilden, Germany). Post extraction, total RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions.