title: GSM8491846: 32D cell WT 3hr rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted and used for RNA-seq analysis. For RNA sequencing, an equal amount of ERCC RNA spike-in control (Thermo Scientific, Waltham, MA) was added to the total RNA samples as internal control before library construction. RNA lifetime profiling was generated from two biological replicates. For RNA lifetime profiling in LT-HSCs, Smart seq II libraries were prepared by Annoroad Gene Technology (China).