title: GSM8974739: Lot LA82 input from 3D culture at day 8; Homo sapiens; RNA-Seq
library_construction_protocol: Cultures were washed with cyclohexamide diluted in PBS and flash frozen on dry ice prior to homogenization in 400uL of polysome lysis buffer. Homogenized lysates are incubated with anti-HA beads for 18 hours at 4 degrees celsius. RNA was isolated from the magnetic beads with the Qiagen RNeasy Plus Micro Kit (Ref # 74034), the concentration determined using the Qubit RNA High Sensitivity Kit) Ref# Q32853 C), and the RIN score calculated using the Agilent Technologies RNA Pico Chips for use with the Agilent 2100 Bioanalyzer system (#5057-1513). Only RIN scores greater than 7 were used for library preparation at Genewiz/Azenta. Library preparation by Azenta Life Sciences; https://www.genewiz.com/public/services/next-generation-sequencing/rna-seq