title: 16s rRNA gene sequence of chicken: broiler cecum
design: Chicks were randomly distributed into treatments (n=6/treatment): Negative control (NC; standard diet [SD], no SH challenge), Positive Control (PC; SD, +SH challenge), antibiotic group (AB; SD with 50g/ton bacitracin +SH challenge), CA (SD with CA 1% v/w, +SH challenge). All groups except NC were challenged with SH (3.8 log10 CFU/bird) on the 4th week, and cecal content samples were collected on the 5th week. Microbial DNA extraction was done using DNeasy powersoil pro kit. Library preparation and sequencing of V4 region of 16s rRNA gene was done using Illumina Miseq 2x 300 platform