title: ATAC-Seq of Oryctolagus cuniculus: adult male testis
design: Testis tissues were ground into powder in a pre-cooled lysis buffer. Nuclei was subsequently isolated by centrifugation at 550 rcf for 10min at 4 C and the nuclei suspensions were resuspended in the Tn5 transposase reaction mixture, then incubated at 37 C for 1 h. Following transposition, the DNA libraries were amplified using PCR and then purified.