title: GSM9622132: HaCaT-derived exosome, ES group, biol rep 2; Homo sapiens; miRNA-Seq
library_construction_protocol: Collected media were centrifuged at 500 × g for 10 min and 2,000 × g for 20 min, filtered through a 0.2 μm membrane, concentrated by TFF, and ultracentrifuged at 100,000 × g for 2 h at 4 °C for exosome isolation. Exosomal RNA was harvested using miRNeasy® Serum/Plasma Advanced Kit. The experiment was performed according to the manufacturer's instructions. RNA extraction was performed by Macrogen Co. Ltd., Republic of Korea. Small RNA libraries were prepared using SMARTer Small RNA library and NEBNext Small RNA library and sequenced on NovaSeqX. Library construction was performed by Macrogen Co. Ltd., Republic of Korea.