title: GSM9326705: scRNASeq_Mulitplexed_SubLibrary_JR1; Mus musculus; RNA-Seq
library_construction_protocol: The colons were opened and washed with PBS. Epithelial cells were isolated by incubating the tissue in 15 mM EDTA for 45 minutes at 37°C, followed by manual shaking. Crypts were dissociated into single cells by incubation in a dissociation buffer containing Dispase II (2 U/mL) and DNase I (30 μg/mL) for 30 minutes at 37°C. Cells were pooled by condition and sorted at a 1:1 ratio of proliferative epithelial cells (EPCAM⁺, CD44⁺, CD45⁻) and differentiated epithelial cells (EPCAM⁺, CD44⁻, CD45⁻) using fluorescence-activated cell sorting (FACS). The library was prepared using the Parse Biosciences Evercode WT Library Kit according to the manufacturer's protocol.