title: GSM9329684: Nasopharyngeal sample – TN_511_501; human metagenome; OTHER
library_construction_protocol: Total nucleic acids were extracted from nasopharyngeal swab samples using the Mag-Bind® Viral DNA/RNA 96 Kit (Omega Bio-Tek) following the manufacturer's protocol. Samples were stored at −80 °C prior to extraction. The V3–V4 hypervariable region of the bacterial 16S rRNA gene was amplified using primers 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 806R (5′-GGACTACHVGGGTWTCTAAT-3′). Amplicons were prepared for sequencing using Illumina MiSeq library construction workflows by the Environmental Sample Preparation and Sequencing Facility (Argonne National Laboratory). Sequencing was performed using Illumina MiSeq paired-end chemistry.