title: GSM9220658: mT23-2D, JQ1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in 1 ml of TRIzol reagent (Thermo Fisher Scientific, 15596026) at room temperature for 5 minutes with gentle agitation, followed by the addition of 200 µl of chloroform (Reagents, C755N59), according to the manufacturer's protocol. RNA was extracted using the PureLink RNA Mini Kit (Invitrogen, 12183018A) and quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific) prior to sequencing submission. Library preparation and sequencing for RNA-seq was carried out by Novogene Co., LTD. Total RNA quality was assessed using 1% agarose gel electrophoresis, NanoDrop spectrophotometry (for RNA quantity and purity), and an Agilent 2100 Bioanalyzer (for RNA Integrity Number, RIN). Poly(A) mRNA was enriched from total RNA using oligo(dT) magnetic beads, fragmented, and reverse-transcribed to synthesize first-strand cDNA with random primers. Second-strand cDNA synthesis was performed using a dUTP-incorporation protocol to maintain strand specificity. The resulting double-stranded cDNA was end-repaired, A-tailed, and ligated to Illumina adapters, followed by size selection and PCR amplification. Libraries were quantified and quality-checked prior to sequencing.