title: RNA-seq of Trimma hollemani muscle tissue
design: Tissues were extracted, flash-frozen in liquid nitrogen and stored at -80 C. Tissues samples were shipped to Novogene (https://www.novogene.com/us-en/) for RNA extraction and reference-based sequencing. Total mRNA was isolated using the poly A enrichment method, where poly-T oligo-attached magnetic beads are used to pull down the polyadenylated (poly-A) tail of mRNA.Sequencing was conducted on an Illumina NovaSeq platform using 150 bp paired-end reads.