title: UCE bait-capture genomic sequencing of R. magnifica
design: For each sample, up to 1000ng of genomic DNA was carried forward for library preparation, which was sheared to a target fragment size of 400-800bp using a Covaris Ultrasonicator. Library preparation was performed using a Kapa Hyper Prep Kit for bait-capture sequencing, with universal Y-yoke oligonucleotide adapters and iTru dual-indexed primers. Libraries were pooled into equimolar ratios (100ng) and target enrichment was performed using MyBaits v.IV protocol using a 500ng/rxn concentration of baits. Bait-capture enriched libraries were sequenced at Arbor BioSciences on an Illumina NovaSeq using 150bp paired-end sequencing.