title: GSM9504985: s227; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from ~50 mg placental tissue using TRIzol reagent following manufacturer's protocol. RNA quality and quantity were assessed using Agilent 2100 Bioanalyzer (RIN>7). Libraries were prepared using NEBNext Ultra II RNA Library Prep with polyA selection. mRNA was enriched with Oligo d(T) beads, fragmented at 94 degrees C for 15 min, converted to cDNA, end-repaired, adenylated, adapter-ligated, and PCR-amplified. Libraries were validated on Agilent TapeStation and quantified by Qubit and qPCR.