title: GSM8521123: C8_KO_Tala; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using GeneJet purification kit (K0731, ThermoFisher), according to manufacturer's instructions. 200 ng was sent for seqencing. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.