title: Metagenome of intestinal bacteria of Awang sheep
design: The total genomic DNA from the samples was extracted using the QIAamp PowerFecal Pro DNA Kit (Qiagen, Inc., German) according to the manufacturers instructions. DNA concentration and integrity were assessed by a NanoDrop2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. DNA was fragmented by S220 Focused-ultrasonicators (Covaris, USA) and purified with Agencourt AMPure XP beads (Beckman Coulter Co., USA). Then the libraries were constructed using TruSeq Nano DNA LT Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturers instructions. The Metagenome sequencing and analysis were conducted by OE Biotech Co., Ltd. (Shanghai, China).