title: GSM9005447: U-2 OS, G3BP1-miniSOG, 5hr poly(I:C) and 3hr 0.5%DMSO, Input, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lyzed with TRIzol. Total RNAs were extracted, clicked with biotin handles, enriched with streptavidin-coated beads, and purified again with TRIzol. For the study of cancer cell migration, cells with strong biotin signals were sorted by flow cytometry and lyzed with 0.5% (v/v) Triton X-100 and 0.1 U DNase I. For the profiling of subcellular transcriptomes, less than 100 ng Input RNA samples or 5 μL Enrich RNA samples were utilized for NGS library construction. NEBNext Ultra II RNA Library Prep Kit for Illumina was used for NGS library construction. RNAs were fragmentated to ~300 nt before first strand synthesis. Libraries were sequenced to a depth of 15 G raw base.