title: RNA sequencing of human primary neonatal Naive CD4+ T cells and adult Naive CD4+ T cells treated with Scr or LINE1 ASOs
design: RNA sequencing of human primary neonatal Naive CD4+ T cells and adult Naive CD4+ T cells treated with Scr or LINE1 ASOs
library_construction_protocol: Naïve CD4+ T cells have been isolated from PBMCs (umbilical for neonatal t cells and peripheral for adult t cells) by density gradient centrifugation with Ficoll-Paque Plus. From PBMCs Naive CD4+ were FACS sorted as CD4+ CD45RA+ CD45RO- Adult naive CD4+ T cells were cultured with LINE1 or scramble (control) ASOs RNA has been extracted with Maxwell RCS simplyRNA Cells kit Libraries were prepared using Illumina Stranded mRNA prep kit and following manufacturer's guidelines