title: RNA-Seq of Chinses cabbage : leaf
design: Total RNA was extracted from leaf tissues using the Trizol reagent kit according to the manufacturers protocol. RNA quality was assessed and checked by Agilent 2100 Bioanalyzer, NanoPhotometer spectrophotometer, and RNase free agarose gel electrophoresis. Eighteen libraries of RNA-seq were constructed using NEBNext RNA Library Prep Kit (NEB # E7530) following the manufacturers instructions. All cDNA libraries were checked by the Agilent 2100 bioanalyzer, NanoPhotometer spectrophotometer, and Qubit2.0 Fluorometer. After passing the quality inspection, eighteen libraries were sequenced on the Illumina sequencing platform (Illumina HiSeqTM4000) using 150 bp paired-end sequencing by Gene Denovo Biotechnology Co. (Guangzhou, China).