title: GSM9662196: IgG; Mus musculus; OTHER
library_construction_protocol: CUT&Tag was performed with Hyperactive Universal CUT&Tag Assay Kit for for Illumina Pro (TD904, Vazyme) according to the manufacturer's instructions. In brief, BV2 cells were collected and counted same number for each group. Nuclei were isolated from BV2 cells and bounded to Concanavalin A (ConA)-coated beads. Subsequently, Nuclei were resuspended in antibody buffer and incubated with primary antibodies against H3K14ac (rabbit, Cell Signaling Technology, #7627), H3K4me3 (rabbit, Cell Signaling Technology, #9751) and IgG (rabbit, Cell Signaling Technology, #66362) at 4 °C overnight. On the next day, samples were incubated with goat anti-rabbit secondary antibodies (1:50, Cell Signaling Technology, #35401). The samples were incubated with pA/G-Tn5 transposase. After transposon activation and fragmentation, 0.5 pg Spike-in DNA was added to each sample and total DNA was isolated, amplified, and purified to construct library. The library for sequencing was constructed with TruePrep Index Kit V2 for Illumina (TD202, Vazyme) and VAHTS DNA Clean Beads (N411, Vazyme) were used for purification steps.