title: GSM8951846: U2OS WT, DMSO, 16 h, Riboseq; Homo sapiens; OTHER
library_construction_protocol: RiboSeq: Cytoplasmic RNA from ribosome protected fragments (RPFs) were isolated from cytosolic cell lysate. Isolates were RNaseI-treated  and monosomes were purified using TRIzol reagent and chloroform. RNA-seq:Cytoplasmic RNA was isolated from cytosolic cell lysate. RNA was extracted by Trizol-chloroform extraction and isopropanol precipitation. RiboSeq: Libraries were constructed according to McGlincy NJ and Ingolia NT.  Methods. 2017 RNA-seq:Libraries were constructed according Loayza-Puch, F., Drost, J., Rooijers, K. et al.   Genome Biology 2013