title: MPXV Clade Ia sequencing using MBS 2500 bp primers
design: Amplicons were generated from a high-titre skin lesion swab sample (lowest cycle threshold) collected on February 5, 2025. These were sequenced using the Illumina COVIDSeq assay as previously described2, producing 2  150 bp paired-end reads over 300 cycles on the NextSeq1000 platform with XLEAP-SBS chemistry and a P1 flow cell. We used the 2500bp Welkers et al. scheme (https://www.protocols.io/view/monkeypox-virus-whole-genome-sequencing-using-comb-n2bvj6155lk5/v1?step=1).