title: Genome-wide study of the role of Tox4 on gene expression in adult Drosophila ovaries
design: Genome-wide study of the role of Tox4 on gene expression in adult Drosophila ovaries
library_construction_protocol: Ovaries were dissected in 1 x PBS and then transferred into TRIzol reagent. Flies were grown at 25°C on standard media containing maize, sugar, yeast, agar and anti-fungal agents.  RNA was extracted from 10 pairs of ovary/sample. Total RNA was extracted according to the manufacturer's instructions and RNA was stored in RNAse free water. Quantity and quality of RNA was quantified using Nanodrop ND-2000 (Thermo Fisher Scientific) and Agilent TapeStation system (Agilent). RNA-sequencing libraries were constructed using the Stranded Total RNA Prep. Ligation with Ribo-Zero Plus kit (Illumina, Inc.) according to the manufacturer's protocol, except with a hybridization step using customised oligonucleotide probes for depletion of Drosophila rRNA.