title: RNA-seq of zmcuc1 zmcuc2 zmcuc3 mutant
design: For RNA isolation, the shoot apex including the one newly formed leaf primordia (SAM+ P1) from more than 10 seedlings (10 days old) were dissected for each biological replicate, and more than three biological replicates were prepared for zmcuc1-1/+ zmcuc2-1 zmcuc3-1, zmcuc triple mutants and the segregated wild type siblings. The total RNA was extracted using Monarch Total RNA Miniprep Kit (NEB). cDNA libraries were constructed following the standard Illumina protocol and followed by sequencing on the Illumina platform at the National Key Laboratory of Crop Genetic Improvement in Huazhong Agricultural University.