title: 16S_V3V4-seq of Bacteria: overlying water of Lake Taihu
design: Microbiome bioinformatics were performed with QIIME 2 2019.4 (Bolyen et al. 2018) with slight modification according to the official tutorials (https://docs.qiime2.org/2019.4/tutorials/). Briefly, raw sequence data were demultiplexed using the demux plugin following by primers cutting with cutadapt plugin (Martin, M., 2011). Sequences were then quality filtered, denoised, merged and chimera removed using the DADA2 plugin (Callahan et al. 2016). Non-singleton amplicon sequence variants (ASVs) were aligned with mafft (Katoh et al. 2002) and used to construct a phylogeny with fasttree2 (Price et al. 2010).