title: GSM8801412: Alpha Beta T cell Donor 2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Rneasy Minikit (Qiagen) and quality and conc were determined using TapeStation (Agilent) NEBNext Ultra II RNA Library Prep Kit.Briefly, mRNAs were initially enriched with Oligod(T) beads.  Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNA were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by PCR with limited cycles. The sequencing library was validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).