title: Illumina DNA short reads
design: We pooled seven genetically identical adult unwinged aphids cultivated in the laboratory and isolated genomic DNA (gDNA) using a bender-buffer extraction, resulting in 167.4 ng/ul of gDNA in 59 ul 260/280= 2.09 260/230=2.21. We then sheared the gDNA to approximately 20kb fragments using Covaris G-tubes (Covaris LLC., Woburn, MA, USA) at 4200 RMP for 1 minute, followed by tube inversion. For library preparation, we used the NEB Next PPFE repair kit with Ultra II end prep reaction (New England Biolabs, Ipswich, MA, USA) under recommended conditions and Nanopore ligation sequencing kit SQK-LSK110. For sequencing, we used a Nanopore R9.4.1 (FLO-MIN106D) flow cell and a MinION MIN-101B sequencing device (Oxford Nanopore Technologies, Oxford, UK). We ran the flow cell for 24 hours, followed by a wash with Flow Cell Wash Kit (EXP-WSH004); we then reloaded the flow cell with a second library prep and ran the sequencer for an additional 48 hours. We stopped the second sequencing run at 24 hours (~11 Gbps of sequencing).