title: WT and heterozygous Gata2-mutant mouse HSCs
design: WT and heterozygous Gata2-mutant mouse HSCs
library_construction_protocol: WT or GATA2 Animals were housed under specific-pathogen-free conditions. All experiments were performed in accordance with animal welfare regulations. 3x10^6 freshly isolated nucleated BM CD45.2+ cells from 65-week-old WT or Gata2+/- mice were transplanted via tail vein injection into lethally irradiated (10.5Gy) CD45.1+ WT recipient mice. RNA was isolated using Trizol  cDNA was smarter amplified