title: GSM9087086: WT CD4+CCR7+ thymocytes; Mus musculus; RNA-Seq
library_construction_protocol: Thymocytes from Cd4Cre mice and Cd4Cre Cgasfl/fl flow mice were isolated. The resulting cells were filtered through 100-μm cell strainers, washed with PBS, lysed in red blood cell buffer, and resuspended in PBS. CCR7+ Thymocytes were sorted using a FACSAria Fusion flow cytometer (BD Biosciences). The cell suspension (300–600 viable cells per mL) was loaded onto a Chromium Single Cell Controller (10x Genomics) to generate single-cell gel beads in emulsion, following the manufacturer's protocol. TCR libraries were prepared using the TCR kit and transcriptome libraries was generated by Single Cell 3' Library and Gel Bead kit and sequenced on an Illumina NovaSeq 6000 instrument with a paired-end 150-base pair (PE150) reading strategy.