title: RNA-seq of orestias ascotanensis: winter gills
design: Total RNA was extracted from three different tissue (gills, skin, and muscle) samples obtained from each of the 14 individuals captured during either summer (n=7) or winter (n=7), using TRIzol (Invitrogen) following the manufacturer's protocol. The concentration and quality of the extracted RNA were assessed using a Qubit fluorometer (Invitrogen) and a Bioanalyzer 2100 (Agilent), determining the integrity and purity of each sample. Unstranded poly-A libraries were prepared from each tissue sample using the Illumina TruSeq RNA sample preparation kit (Illumina), following the manufacturer's instructions. The libraries were sequenced on the Illumina Hiseq 4000 platform, generating 150 bp paired-end reads.