title: GSM9328283: 18-hour transmigrated monocytes from healthy donor 13 (D13_CF_ArMos); Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from the cells using TriPure Isolation Reagent mixed with chloroform 4:1.  After spinning, the aqueous phase is mixed 1:1 with 200 proof EtOH.  The mixture is then processed following the protocol of RNA Clean and Concentrator -5 (ZYMO Research).  RNA quality and concentration was quantified using a Bioanalyzer (Agilent technologies, Inc.) and only samples with RIN >5.0 were used for sequencing. cDNA was generated using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara Bio) and the final sequencing library is made using the Nextera XT kit (Illumina) with an aim of ~32 million reads per sample.