title: dsRNA-seq of Drosophila: adult testis
design: dsRNA was immunoprecipitated following Dhir et al., 2018. 10% of the supernatant was reserved as input control. Total RNA was isolated from dsRIP and lysate using TRIzol. rRNA depletion was performed using the Qiagen FastSelect rRNA Fly kit. Libraries were generated using the SMARTer Stranded Total RNA-Seq Kit for low input. Sequencing was performed with 150 bp paired-end reads.