title: RNA-Seq of  Oryza sativa: Jupiter Salt Stress at 120 mM NaCl for 24h BR2
design: Salt stress (120 mM NaCl) was imposed on the seedlings at the Two-leaf  stage of  Oryza sativa cv. Jupiter and leaf samples were harvested at 24 h. Total RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA, USA) and  purity was checked using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.