title: GSM8974984: J3576_m; Mus musculus; RNA-Seq
library_construction_protocol: Brain: 30 mg brain sections were subjected to RLT buffer from RNeasy Mini Kit (Qiagen 74106) with 10% beta-mercaptoethanol added and passed through a 25G needle. Homogenate was transferred to QIAshredder (Qiagen 79656) for further dissociation. The remaining manufacturers protocol was followed to complete the RNA isolation. Quality and concentration were analyzed via RNA Nano 600 kit for Agilent 2100 Bioanalyzer (5067-1511). Muscle: 50 mg muscle sections were powdered by the addition of N2 and manually dissociated via mortar and pestle. QIAzol (Qiagen 5,346,994) was added to the powdered muscle and passed through a 25G needle. Homogenate was added to QIAshredder and the RNeasy Mini Kit protocol was followed for the duration of RNA isolation. Quality and concentration were analyzed via RNA Nano 600 kit for Agilent 2100 Bioanalyzer (5067-1511). Brain and Muscle: Extracted RNA was sent to Novogene Co. located in Sacramento CA, for generation of total RNA libraries and sequencing on NovaSeq X Plus Series (PE150) machine. Data was received in .fq.gz formatting.