title: genome sequencing of the  Lacticaseibacillus paracasei G277-1.1, a Caries-Associated Strain with Enhanced Biofilm Synergy with Streptococcus mutans
design: To prepare for long-read sequencing, 3.5 g of genomic DNA was sheared using the Megaruptor 3 system, configured to 30 cycles of up-and-down motion at speed 29 with 10-second pauses, targeting fragments of 4050 kb. Fragment size distribution was confirmed using the Agilent TapeStation prior to library preparation.The sheared DNA was processed into a barcoded SMRTbell library using the PacBio SMRTbell prep kit 3.0, incorporating barcoding adapters. Post-library construction, binding, cleanup, and polymerase treatment were performed with the Revio reagent kit, followed by sequencing on the PacBio Revio platform.