title: Small RNA-Seq for primary rat hepatic stellate cells and hepatic stellate cell-derived small extracellular vesicles
design: Small RNA-Seq for primary rat hepatic stellate cells and hepatic stellate cell-derived small extracellular vesicles
library_construction_protocol: Hepatic stellate cells were obtained by a two-step enzyme digestion protocol and further purified by magnetic-activated cell sorting (MACS) negative selection (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany) to remove possible contamination of CD45+ myeloid cells and CD31+ endothelial cells according to the manufacturer's instructions.  Hepatic stellate cells derived sEVs were obtained using an ExoQuick kit (System Biosciences, Inc.). TRIzol (Life Technologies) Illumina TruSeq Small RNA Sample kit (Illumina)