title: scRNA-Seq of homo sapiens: adult bone marrow
design: Single cell encapsulation performed as per manufacturer's instructions. GEM-X single cell 3', protocol CG000731 Rev A (or Next-GEM single cell 3' v3.1, protocol CG000315 Rev E). Briefly, cells encapsulated with v4 (or v3.1) reagents; GEM-RT incubation; then clean-up, cDNA amplification (13 cycles of PCR) and cDNA clean-up. Samples were quantified with Agilent TapeStation. 10 ng of cDNA library then taken through to legacy Oxford Nanopore (ONT) SQK-PCS111 protocol, using PCS114 reagents, where cDNA biotin-tagged, followed by Streptavidin bead pull down before final PCR with clean up. Adapters then added, and Promethion R10.4.1 flow cell loaded (or R9 chemistry for OMB1791, 1798, 1846), one sample per flow cell. Samples were sequenced on a Promethion 48, with super accurate base calling.