title: RNA-seq of Pseudostellaria heterophylla :rhizosphere soil microbial community structure
design: DNA was extracted using the TGuide S96 Magnetic Soil/Stool DNA Kit (Tiangen Biotech Co., Ltd., Beijing, China) according to the manufacturer's instructions. DNA concentration  was measured using a Qubit dsDNA High Sensitivity Assay Kit on a Qubit 4.0 Fluorometer (Invitrogen, Thermo Fisher Scientific, USA). Amplicons were purified with Agencourt AMPure XP Beads (Beckman Coulter, USA), quantified as above, and sequenced on a PacBio Sequel II 8 M SMRT cell using the Sequel II Sequencing Kit (v 2.0).