title: GSM9005871: Sample 1002 CD45+ cells, TCR; Homo sapiens; OTHER
library_construction_protocol: Human peripheral blood mononuclear cells (PBMC) from participants of the clinical study were isolated and thawed using prewarmed BD BSA stain buffer (BD Biosciences), washed twice with ice-cold buffer and counted Single cells were encapsulated into nanoliter-scale Gel Beads-in-emulsion (GEMs) using Chromium Controller (10X Genomics) following manufacture's protocol. Chromium Single Cell 5' Library & Gel Bead, Single Cell V(D)J Enrichment Kit, Mouse T Cell, and Single Cell 5' Feature Barcode kits were used to prepare single cell 5' RNA, single cell TCR, and single cell surface protein libraries following manufacture's protocol. The quality and quantity of the libraries were assessed with Agilent TapeStation (Agilent Technologies) and Qubit Fluorometer (Thermo Fisher Scientific).