title: HDA5_BB633-03T0008_good_1
design: To identify genes which were involved in histone deacetylation regulates lipid through H4K5 and H4K8. The worms which fed with E. coli hda 5RNAi until reach to adult. After washing by M9 buffer for three times, worms were re-suspended in PBS, frozen immediately in liquid nitrogen for RNA sequencing which were carried out by Biomarker Technologies (BMK) GmbH (Beijing, China). Total RNA from C. elegans was extracted with RNeasy Plus Mini Kit (Qiagen), assessed for quality by NanoDrop, Qubit 2.0 and Agilent 2100. mRNA was enriched via Oligo(dT) magnetic beads, fragmented, and reverse-transcribed to cDNA with random hexamers. After end repair, A-tailing, adapter ligation and PCR amplification, libraries were qualified by Qubit 2.0, Agilent 2100 and Q-PCR, then sequenced on Illumina NovaSeq 6000 (PE150).