title: RADSeq of Cardamine C224_102
design: Total genomic DNA was extracted from silica gel-dried leaves using theExgene Plant SV kit(GeneAll, Seoul, Korea) and purified with0.4 AMPure XP beads(Beckman Coulter, Indianapolis, USA). The double-digest RADseq (ddRADseq) libraries were prepared following the protocol ofPeterson et al. (2012), with modifications as described in detail bylenker et al. (2024). Libraries were sequenced with150-bp paired-end readson Illumina systems atNovogene(Cambridge, UK).