title: H27-3 (CRX990586; CRA015402)
library_construction_protocol: Total RNA was extracted using Trizol reagent (Sigma-Aldrich, USA). Strand-specific RNA-seq libraries were prepared using the VAHTS Stranded mRNA-seq Library Prep Kit for Illumina v2 (Vazyme Biotech, China) and subjected to 150bp paired-end sequencing on the Illumina Novaseq 6000 platform. Trimmomatic software was employed to remove low-quality reads and sequencing adapters, followed by the alignment of clean reads to the zebrafish reference genome GRCz11 (v106) using STAR software. Read counts for each gene were calculated using featureCounts. Finally, fragments per kilobase million (FPKM) values for each gene were obtained using DESeq6.