title: sample0994
design: Primers of two hypervariable regions (V3-V4) of the 16S rRNA gene were used. Read assembly and cleanup were derived from the MiSeq platform described by the Schloss lab (26). All sequences classified as Eukaryota, Archaea, Chloroplasts and Mitochondria, and those that could not be classified, were removed. Contigs were clustered into OTUs at 97% sequence similarity, and the most abundant sequence of each OTU was selected as the representative sequence. Reads with a maximum abundance of 5 across samples were removed, as they were supposedly artefacts or bacteria that did not have any biological impact.