title: adult ticks of Ixodes persulcatus
design: he PE reads obtained by Illumina PE250 sequencing were first spliced according to the overlap relationship. At the same time, the quality of the sequence was controlled and filtered. After distinguishing the samples, OTU cluster analysis and species taxonomy analysis were performed. Based on the results of OTU cluster analysis, OTU diversity index analysis and sequencing depth detection can be performed. Based on taxonomic information, statistical analysis of community structure can be performed at various taxonomic levels. On the basis of the above analysis, a series of in-depth statistical and visual analysis of community structure and phylogeny can be carried out.