title: GSM9662337: BMDM from Tnfsf4^Lyz2-OE mice (p53); Mus musculus; OTHER
library_construction_protocol: For each CUT&Tag-seq experiment, 0.1 million BMDMs were processed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Vazyme Biotech, TD904) according to the manufacturer's instructions. Cells were incubated with a p53-specific antibody or rabbit IgG followed by secondary antibody and pA-Tn5 transposome to enable targeted tagmentation. CUT&Tag-seq libraries were generated using the same kit, and indexed using the TruePrep Index Kit V2 for Illumina (Vazyme Biotech, TD202) according to the manufacturer's protocol. Libraries were amplified by PCR, purified, and subjected to high-throughput sequencing.