title: RNA sequence analysis of Arabidopsis thaliana : the role of mRNA methylation in control of seed germination
design: Total RNA was isolated from  te234 mutant dry seed using TRI Reagent (Sigma) followed by chloroform separation and isopropanol precipitation. Poly(A) mRNA was enriched with oligo(dT) beads, fragmented, and converted to strand-specific Illumina libraries via random-primed cDNA synthesis, end-repair, A-tailing, adapter ligation, size selection (250300 bp inserts), and limited-cycle PCR. Libraries were sequenced as 2  150 bp paired-end reads on an Illumina platform (Novogene, Q30  84%). The complete experiment was performed once with three biological replicates per genotype.