title: RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in the abscence of lsy-2 activity
design: RNA-seq of C. elegans young adult hermaphrodites to detect differentially expressed genes in the abscence of lsy-2 activity
library_construction_protocol: At least 1000 animals from wild-type and lsy-2(ot64) strains were synchronized and collected as young adult hermaphrodites 50 hours post-larval hatching after hypochlorite treatment, at 20Â°C (Stiernagle, T. 2006). Total RNA was extracted with TRIzol Reagent (Invitrogen; Carlsbad CA, USA) and RNA was purified using the PureLink RNA Mini Kit (Invitrogen; Carlsbad IA, USA) according to the manufacturer's instructions cDNA libraries were built using the TruSeq stranded mRNA library preparation kit (Illumina Inc.; San Diego CA, USA) by the Next Generation Sequencing (NGS) Platform in Bern (https://www.ngs.unibe.ch/)