title: Prenatal LPS infection impacting on neonatal mice lung at birth
design: Prenatal LPS infection impacting on neonatal mice lung at birth
library_construction_protocol: Cranial and accessory lobes of the right lung were removed and frozen in liquid nitrogen RNA was extracted using the RNeasy Mini Kit automatically via QIAcube®Connect (Qiagen, Hamburg, Germany). 500 ng of RNA per sample was used to enrich for polyadenylated mRNA followed by cDNA sequencing library preparation utilizing the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®.