title: RNA-Seq of mus musculus:female skin
design: mRNA was enriched from total RNA using Oligo dT magnetic beads. After fragmentation, first-strand cDNA was synthesized using random hexamer primers. Then, second-strand cDNA was synthesized using dUTP instead of dTTP. After end repair, A-tailing, adapter ligation, fragment selection, amplification and purification, the strand-specific library was ready.