title: 16S amplicon sequencing of medicago root microbiota sample CPLYK9R2 (CRX960091; CRA014900)
library_construction_protocol: DNA for each sample was extracted with FastDNA SPIN Kit (MP Biomedicals), then V5-V7(799F-1193R) of 16S rDNA was amplified by two-step PCR. Finally, the amplicons were sequenced on Illumina HiSeq 2500 platform in pair-end 250 bp mode.