title: vRNAseq of SIV-CoV2 infected macaca mulatta: adult female nasal swab
design: Viral RNA was extracted using Quick-RNA Viral Kit (Zymo Research) and cDNA synthesized with SuperScript IV (Invitrogen). PCR tiling was performed with the xGen SARS-CoV-2 Amplicon Panels (IDT). Libraries were prepared with TruSeq Stranded Total RNA kit and barcoded using the Nextera XT Index Kit (Illumina). Pooled samples were purified with AMPure XP beads and sequenced on the Illumina NextSeq platform.