title: RNA-seq of Ixodes scapularis adult female salivary glands during feeding on rabbits
design: Intact salivary glands (SG) from partially fed Ixodes scapularis adult females feeding on rabbits were collected and cells were dissociated following a mechanical and enzymatic protocol. Ticks were grouped by weight, the average weight of the group 2 is 7.4 mg. RNA extraction was performed with the Arcturus PicoPure RNA isolation kit (Thermo Fisher Scientific) according to manufacturer`s specifications and analyzed with an TapeStation system (Agilent, USA). The library was constructed using the NEBNextUltraTM II (Directional) RNA with polyA selection library prep kit and sequencing was performed in an Illumina Novaseq 6000 DNA sequencer.