title: WGS with Illumina of Epinephelus itajara: adult male
design: DNA extraction was performed at the Laboratory of Molecular Ecology of Aquatic Vertebrates, Universidad de los Andes (Colombia). The QIAGEN DNeasy Blood & Tissue Kit (50) was used to extract genomic DNA. DNA quality and quantity were assessed using Qubit and Nanodrop, and integrity was verified by electrophoresis on a 0.8% agarose gel. Whole-genome sequencing libraries were prepared and sequenced by SNPsaurus LLC (Eugene, Oregon, USA) using the Illumina NovaSeq X platform following their standard library preparation and sequencing protocols.