title: GSM8640972: ME49,HDAC3,biol rep 2; Toxoplasma gondii; OTHER
library_construction_protocol: Intracellular parasites were released by needle passage and purified through filtration with 3 μm pore-size membranes. Approximately 5 x 10⁶ tachyzoites were collected and processed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina (Vazyme Biotech, China), following the manufacturer's instructions. The resulting DNA was amplified for library construction using the TD202 TruePrep Index Kit V2 for Illumina (Vazyme Biotech, China). The libraries were enriched, quantified, and sequenced on a NovaSeq 6000 sequencer (Illumina) using the PE150 model.