title: GSM9618476: MFascicularis_exp3_chip2_GEX; Macaca fascicularis; RNA-Seq
library_construction_protocol: To quantify mRNA and gRNAs of the cells, cells were dissociated to single cells using Accumax, washed and stored in PBS +0.04 % Bsa. Human and cynomolgus cells were pooled to equal cell numbers per cell line, then the single cell suspension was loaded on a 10x Genomics Chromium Controller for GEM generation. RT and cDNA amplification were conducted according to the manufacturer's instructions (Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) with Feature Barcode technology for CRISPR Screening; USER GUIDE: CG000510 Rev B). We performed three CRISPRi screens in total, using one 10x chip in the first two and three 10x chips in the third experiment (5 batches in total). Per 10x chip and lane, two libraries were generated: a gene expression library and a CRISPR library (identified as GEX and CRISPR lib in the sample names). For details see the manufacturer's protocol.