title: O_SCI2
design: Total RNA was extracted from tissue samples using TRIzol Reagent, and the quality was assessed using the 5300 Bioanalyzer (Agilent). Quantification of the extracted RNA was performed with the ND-2000 (NanoDrop Technologies). High-quality RNA samples (OD260/280 = 1.82.2, OD260/230  2.0, RIN  6.5, 28S:18S  1.0, > 1 g) were utilized for library construction. RNA purification, reverse transcription, library preparation, and sequencing were carried out at Shanghai MajorBio Bio-pharm Biotechnology Co. Ltd., following Illumina protocols. The RNA-seq library was prepared using 1 g of total RNA, involving mRNA isolation with poly-A selection, cDNA synthesis with random hexamer primers, and end-repair with 'A' base addition. The size-selected libraries (300 bp) were then PCR amplified and sequenced on a NovaSeq 6000 sequencer (2  150 bp).