title: RNA-seq of Tenmnothorax nylanderi: fat body
design: Anomotaenia brevis infected ant, not LPS treated. We isolated the fat body from each worker by removing the gut and trachea from the first gaster segment, leaving only the fat cells, which were placed in a vial containing 50 l Trizol. Samples were frozen on dry ice and subsequently transferred to -80C.  RNA was extracted using the Qiagen RNeasy extraction kit. We sent the samples to Novogene (Cambridge, UK) for mRNA library preparation using a NEBNext Ultra RNA Library Prep Kit. The libraries were sequenced on an Illumina Novaseq 6000 in paired-end 150 bp mode, yielding a minimum of 9 Gb per sample.