title: Genome-wide DNA methylation sequencing
design: Lambda DNA was added to the sample DNA as a positive control for bisulfite conversion. The fragmented DNA samples by using sonication were subjected to bisulfite conversion. The Accel-NGS Methyl-Seq DNA Library Kit (Swift, MI, USA) was utilized for attaching adapters to single-stranded DNA fragments. Finally, pair-end 200 bp sequencing was conducted on the Illumina Hiseq 4000 platform.