title: GSM8975152: CHRF_CUTRUN_Menin; Homo sapiens; OTHER
library_construction_protocol: CUT&RUN samples were prepared using the CUTANA ChIC/CUT&RUN kit. For scRNAseq, leukemic cells were human leukemic cells were isolated from the spleens of PDX-bearing mice treated for 5 days, washed three times in PBS + 0.04% BSA, and counted.  The volume of cells to load to have a desired recovery target of 8,000-10,000 cells was calculated and cells were loaded on Chromium Next GEM Chip K (10X Genomics).  Samples were processed using the 10X Genomics 5' kit (v2). CUT&RUN libraries were prepared using the CUTANA CUT&RUN library prep kit.  Single cell RNA-seq libraries were prepared using Chromium Next GEM Single Cell 5' Reagent Kits v2 (10X Genomics) according to manufacturer's protocols.