title: GSM9622292: Day7.organoid.Rep3_Cell_108; Mus musculus; RNA-Seq
library_construction_protocol: Harvested organoids were rinsed in PBS and incubated in 0.25% Trypsin-EDTA at 37°C and 5% CO2 for 10-20 min. After the addition of soybean trypsin inhibitor, the samples were mechanically dissociated. Single cells were manually collected using a glass micropipette (50-60µm inner diameter) and transferred into 96-well plates. SmartSeq2 protocol