title: RNA-Seq of Lilium pensylvanicum
design: Total RNA was isolated from cells using TRIzol reagent (Invitrogen, Waltham, Massachusetts, USA) according to the manufacturers protocol. The quality of the extracted total RNA was measured using Bioanalyzer 2100 (Agilent, SantaClara, CA, USA). Total RNA with an RNA Integrity Number (RIN) value greater than or equal to 7 was used for library preparation. RNA sequencing libraries were prepared using the TruSeq Stranded mRNA LT Sample PrepKit according to the manufacturers instructions (Illumina, SanDiego, CA, USA). A total of nine libraries with the irrespective indices were paired-end sequenced using the HiSeq X system (Illumina).