title: metagenomic sequncing of Mus musculus: gut microbiota
design: DNBSEQ-T7 libraries were constructed from 500 ng of HMW DNA and fragmented using Covaris sonicator E220 (Covaris, Brighton, UK). Sheared DNA underwent End repair and A-tailing steps as described in the MGI Easy Universal DNA Library Prep Set User Manual v6 (MGI Tech Co., Shenzen, China). Adapters ligation was performed following the instructions of the MGIEasy DNA Adapters kit, and cleaned up with the provided DNA Clean Beads. PCR amplification was carried out on purified adapter-ligated DNA and cleaned-up again using magnetic beads. After quality control using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific, Waltham, MD, USA), purified PCR products were denaturated and ligated to generate single-strand circular DNA libraries.