title: GSM9035008: M2; Mus musculus; OTHER
library_construction_protocol: On E9.5, pregnant mice were euthanized by cervical dislocation, and the phenotypes of the fetuses were observed under a microscope and the brain were used for experiments.The brain tissue was digested into a single-cell suspension, and 100,000 cells were used for experiments according to Vazyme #TD903 (Hyperactive Universal CUT&Tag Kit).H3K27me3 (CST #9733) was used for experiments. Amplified libraries using Vazyme HiFi PCR Master Mix (Catalog #TD903),Index primers (Vazyme kit-provided).PCR program:72°C, 5 min; 98°C, 30 sec;12–15 cycles: 98°C 10 sec, 63°C 30 sec, 72°C 30 sec;72°C, 5 min final extension.Library QC:Quantified with Qubit dsDNA HS Assay.Size distribution verified via Bioanalyzer (peak: 200–700 bp).Pooled equimolar libraries for 150-bp paired-end sequencing on Illumina NovaSeq.