title: GSM8733458: SLEC OT-I cell, Rroid2WT, Day 7, replicate2; Mus musculus; RNA-Seq
library_construction_protocol: The OT-I cells were FACS-sorted directly into 700ul RTL lysis buffer supplemented with β-mercaptoethanol. RNA isolation was performed using the RNeasy Micro kit (Qiagen, #74004). RNA-seq libraries were prepared using the SMART-Seq Stranded Kit (#634442) and Unique Dual Index Kit 1–24 (#634756) according to the manufacturer's protocol (Takara Bio).