title: GSM8798843: U2OS-Flp-In-TREx - siNC ChIP anti GFP (Control); Homo sapiens; ChIP-Seq
library_construction_protocol: After siRNA depletion, cells were fixed with 1% formaldehyde for 10 minutes at room temperature and quenched with 20 mM Glycine for 5 min. Post quenching, the cells were lysed and chromatin was sonicated using a Bioruptor (Diagenode) instrument. Libraries were constructed using the NEBNext Ultra II kit following manufacturer's instructions.