title: GSM9621825: hTERT-RPE1 cells, overexpression ccnd1, dox, day 5, rep1; Homo sapiens; RNA-Seq
library_construction_protocol: After PBS wash, cells were lysed using TRIReagent (Zymo Research; R2050-1-50), and RNA was extracted by Direct-zolTM RNA Microprep Kit (Zymo Research; R2051-A). mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490), and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, #E7760) was then used to prepare libraries for paired-end (2x150bp) Illumina sequencing (Novogene). More than 20 million reads were sequenced per sample.