title: GSM8491552: E16.5 wound, day 3 after wounding, rep 1; Mus musculus; RNA-Seq
library_construction_protocol: To collect wound-responsive cells, we utilized a circular punch biopsy that was 1 mm larger than the wounds to collect cells immediately adjacent to the wound as well as those that had migrated into the wound bed. Single-cell suspensions were treated with ACK lysis buffer to remove red blood cells prior to FACS sorting. Live cells (DAPI-negative) were sorted out and used for library construction. 16.5K cells per sample were loaded into the 10X Genomics Chromium Next GEM Chip G. scRNAseq gene expression libraries were generated using the 10X Genomics Chromium Next GEM Single Cell Kit (v3.1) as per manufacturer's protocol. Libraries were sequenced on a NovaSeq 6000 S4 lane.