title: OmniC sequencing data of Choanoeca flexa: whole genome
design: Briefly, chromatin was crosslinked by adding formaldehyde to the cell pellet, after which crosslinked chromatin was extracted and subsequently digested with DNAse I. Chromatin ends were repaired and ligated to a biotinylated bridge adapter, followed by proximity ligation of adapter-containing ends. After that, crosslinks were reversed, and the DNA was purified. Non-ligated or external biotin residues were removed, and sequencing libraries were generated using NEBNext Ultra enzymes and Illumina-compatible adapters. Biotinylated DNA fragments were isolated using streptavidin beads, followed by PCR enrichment of each library. The resulting libraries were sequenced on an Illumina HiSeqX platform, generating approximately 30x sequence coverage. High-quality reads (MQ>50) were used for scaffolding with the HiRise pipeline.