title: GSM9328079: vector,PhiV-1 infection,rep_2; Escherichia coli; RNA-Seq
library_construction_protocol: Total RNA was isolated using the TRIzol methods, including chloroform phase separation, isopropanol precipitation, ethanol washing, and TURBO DNase treatment. RNA quality (RIN>6.5) was identified prior to bacterial rRNA depletion and preparation of strand-specific sequencing libraries. Libraries were sequenced on an Illumina platform, and trimmed reads were aligned to a combined E. coli MG1655 + phiV-1 reference genome for gene-level quantification and differential expression analysis.