title: mRNA-seq and AGO2-RIP-seq of JURKAT T-cell acute lymphoblastic leukemia cell line upon overexpression of hsa-miR-143-3p
design: mRNA-seq and AGO2-RIP-seq of JURKAT T-cell acute lymphoblastic leukemia cell line upon overexpression of hsa-miR-143-3p
library_construction_protocol: For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline. Total RNA was isolated using miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). For each immunoprecipitation reaction 30 million transduced cells were used. The Ago2-RIP was performed using Magna RIP Kit (Merck) and anti-Ago2 antibody (Abnova, H00027161-M01). For total RNA isolation 3 million cultured Jurkat T-ALL cells were harvested and rinsed twice with phosphate buffered saline.