title: GSM9329399: Hep1-6 cell,shNC-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from Hep1-6 cells using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA quality and integrity were assessed using agarose gel electrophoresis and an Agilent 2100 Bioanalyzer. Only high-quality RNA samples were used for library preparation and sequencing. RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs) following the manufacturer's instructions. Poly(A)+ mRNA was purified from total RNA using oligo(dT) magnetic beads, fragmented, and reverse transcribed to cDNA. After end repair, A-tailing, adaptor ligation, and PCR amplification, the final libraries were quantified, pooled, and sequenced on an Illumina platform.