title: GSM9222041: endothelial cells treated with nanoplastics - rep 2; Canis lupus familiaris; RNA-Seq
library_construction_protocol: RNA extraction was performed using the NucleoSpin RNA II kit following manufacturer's instructions (Macherey-Nagel, No. 740955.50). An additional DNA digestion step with rDNase in solution was performed in order to further reduce DNA contamination, followed by RNA precipitation with sodium acetate and ethanol. The Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus kit (Illumina, San Diego, California, USA) was used to prepare transcriptomic libraries using 10 ng of RNA input. Libraries were quantified using Quantus dsDNA kit (Promega, Madison, Wisconsin) and their average fragment length was assessed using Agilent High Sensitivity D1000 kit on the Agilent TapeStation system (Agilent Technologies, Inc, Waldbronn, Germany).