title: PacBio HiFi CI66
design: Leaf tissue was sent to the Arizona Genomics Institute for DNA extraction using a CTAB method (Doyle and Doyle 1987). The sequencing library was constructed using SMRTbell Express Template Prep kit 3.0. The final library was size selected on a Blue Pippin (Sage Science) with 10-25 kb size selection. Sequencing was performed on a PacBio Revio system in CCS mode for 30 hours.