title: Bulk Segregant Analysis of Salt Tolerance Traits in Rice
design: Genomic DNA (gDNA) was extracted from leaves using a gDNA extraction kit (Geneaid Biotech Ltd., New Taipei City, Taiwan) and fragmented with dsDNA Fragmentase (New England Biolabs, Ipswich, MA). The resulting sheared DNA was modified with an End Repaired enzyme (New England Biolabs), and deoxyadenosine was added to the 3 end using a Klenow fragment (New England Biolabs). Each library had unique DNA barcodes (Bioo Scientific, Austin, TX) joined to the DNA using DNA ligase (New England Biolabs). The libraries were sequenced in the Illumina NovaSeq 6000 Sequencer (Illumina, San Diego, CA, USA)