title: GSM8900774: HOM 4; Mus musculus; RNA-Seq
library_construction_protocol: Bulk RNA was extracted using Qiagen Lipid Tissue kit,  from frontal cortex, of wild type and homozygous TardbpM323K/M323K  male mice at 9-months of age (in an  F1 hybrid genetic background, C57BL/6J- DBA/2J). Quality and quantity was assessed using the 5400 Fragment Analyzer System (Agilent Technologies). Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample. The clustering of the index-coded samples was performed on a cBot Cluster Generation System using PE Cluster Kit cBot-HS (Illumina) according to the manufacturer's instructions. After cluster generation, the library preparations were sequenced on an Illumina platform and paired-end reads were generated.