title: GSM8974357: edited_rich_2; Staphylococcus aureus; RNA-Seq
library_construction_protocol: Total RNA was extracted using FastRNA Pro Blue kit (MP Biomedicals) according to the manufacturer's protocol. The samples were treated with DNase I and the quality of the RNA was visualized using agarose gel electrophoresis. Ribosomal RNA (rRNA) was depleted using the S. aureus Ribo-seq riboPOOL kit (SiTOOLs Biotech) according to the manufacturer's instructions. RNA was fragmented using 50 mM bicarbonate buffer for 8 minutes at 96 °C. Fragmented RNA was subsequently treated with dephosphorylation and phosphorylation (deP/P) steps to enable small RNA library construction. Small RNA sequencing libraries were prepared using the NEBNext® Small RNA Library Prep Set for Illumina® (New England Biolabs), following the manufacturer's protocol. Libraries were sequenced on the Illumina NextSeq 1000 platform in the Epitranscriptomics and RNA Sequencing Core, Université de Lorraine.