title: BSA-Seq of Cotton: leaves
design: Their total genomic DNA was extracted using the Hi-DNA secure Plant Kit . The DNA of  plants was mixed evenly to construct  pools, and then sequenced on an Illumina HiSeq 2500 platform. After filtering raw reads, the ensuing high-quality clean reads were aligned to the reference sequences of Gossypium hirsutum using BurrowsWheeler Aligner software. GATK and Picard software tools were used to detect single nucleotide polymorphisms (SNPs), this entailing mark duplication, local realignment, base recalibration variant calling, and SNP filtering.