title: GSM9407031: Wild-type HCT116, technical replicate3; Homo sapiens; RNA-Seq
library_construction_protocol: Total nuclear RNA was isolated from biological and technical replicates using the NucleoSpin RNA Plus Mini kit with on‑column DNase treatment (Macherey‑Nagel, 740984). Polyadenylated RNA was enriched using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB #E7490). Libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (NEB #E7765). Almost 2ug of mRNA was used for library preparation. No size selection was performed during library preparation.