title: rizhospehere microbiome inoculated with P. putida KT2440 WT replicate 2
design: Soil DNA was extracted using the FastDNA Spin Kit for Soil (MP Biomedicals, USA), following the manufacturers protocol with additional modifications. For bacterial 16S rRNA profiling, the extracted soil DNA samples were sent to Macrogen. The V3-V4 regions of the 16S rRNA gene were amplified using Illumina MiSeq paired-end 2x300 system with primers 341F (CCTACGGGNGGCWGCAG) and 805R (GACTACHVGGGTATCTAATCC).