title: GSM8801328: ID15; Homo sapiens; OTHER
library_construction_protocol: Surgical specimens were processed in FFPE in the same way as for conventional pathological analysis.Prior to sectioning, the FFPE blocks were incubated in an ice bath for 10-30 min and 5µm-thick sections were cut using a microtome. Resulting sections were floated for 40 sec in a 42°C water bath until flat and immediately placed onto the Visium slides. Slides containing sections were dried for 3h at 42°C and placed in a desiccator overnight at room temperature to ensure proper drying. They were further stored for a maximum of 1 week before use Deparaffinization, staining, imaging, decrosslinking and construction of sequencing libraries was done according to the manufacturer's instructions (Visium Spatial Gene Expression for FFPE – Deparaffinization, H&E staining, Imaging & decrosslinking, CG000409, RevC; Visium Spatial Gene Expression Reagent Kit for FFPE - User Guide, CG000407, RevD) using the Visium Spatial for FFPE Gene Expression Kit, Human Transcriptome (10x Genomics, 1000338).