title: Long-read Nanopore sequencing of Hydrogenobacter sp. T-2
design: 0.3 g wet cell pellet was used for DNA extraction using a modified version of the JGI bacterial CTAB extraction protocol (https://jgi.doe.gov/user-programs/pmo-overview/protocols-sample-preparation-information/jgi-bacterial-dna-isolation-ctab-protocol-2012/). Modifications to this protocol consisted of three freeze-thaw cycles following the lysozyme treatment, in a dry ice/ethanol bath and a water bath at 65 C for three minutes per cycle, and the use of RNase A (Promega, Madison, WI, USA, catalog number: A7973) for denaturation of ribonucleic acids, and an additional extraction step with chloroform/isoamyl after treatment with RNase A. The sequencing library was prepared with the SQK-LSK109 Ligation Sequencing Kit using the EXP-NBD104 Native Barcoding Expansion Kit (Oxford Nanopore Technologies, Oxford, UK), following the manufacturers instructions and sequenced with a MinION Mk1B device (Oxford Nanopore Technologies, Oxford, UK) with a FLO-MIN106D flow cell.