title: GSM8974359: Foxp3-Cre × CK2b-fl/fl; Mus musculus; OTHER
library_construction_protocol: B16.F10 tumors of comparable size were isolated on day 15 after inoculation B16.F10 tumors of comparable size were isolated on day 15 after inoculation, fixed in formalin and embedded in paraffin. 10µm FFPE embedded sections were cut with a microtome. The spatial transcriptomics workflow was performed using the Visium CytAssist Spatial Gene Expression for FFPE (10X document, Doc ID: CG000520 Rev C). Post-hybridization wash, probe ligation and post-ligation wash were conducted following 10X protocol (DOC ID: CG000495 Rev E). Spatial transcriptome sequencing libraries were prepared with the 10x Genomics workflow according to manufacturer's instructions (10X document: CG000495 Rev E)