title: GSM9444985: KO_E18.5_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from whole embryonic lungs using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions. RNA quality and concentration were assessed prior to library preparation. libraries werRNA-seq libraries were prepared from poly(A)-selected RNA using a standard Illumina-compatible library preparation kit according to the manufacturer's instructions. Libraries were indexed, pooled, and sequenced using paired-end Illumina sequencing.e prepared using the TruSeq Stranded Total RNA LT Kit with Ribo-Zero Gold, according to the manufacturer's instructions.