title: GSM9662344: P16 testis, Hsf5 knockdown, biol rep1; Mus musculus; ncRNA-Seq
library_construction_protocol: Total RNA was used as input material for the RNA sample preparations. Briefly, 3' and 5' adaptors were ligated to 3' and 5' end of small RNA, respectively. Then the first strand cDNA was synthesized after hybridization with reverse transcription primer. The double-stranded cDNA library was generated through PCR enrichment. After purification and size selection, libraries with insertions between18~40 bp were ready for sequencing.