title: GSM8974823: E13.5_Limb, pSmad15_ChIP; Mus musculus; ChIP-Seq
library_construction_protocol: E13.5 limb or molar tissues were fixed with 1% formaldehyde at room temperature for 15 minutes. After stopping fixation and cell lysis, chromatin was sonicated using a Diagenode Bioruptor Sonicator. ChIP was performed following the manufacturer's protocol provided by Active Motif ChIP-IT High Sensitivity Kit. Illuminina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of DNA-end repair, 3'-dA overhang, and adaptor ligation. After PCR amplification and size selection, the resulting DNA libraries were quantified and sequenced with an Illumina HiSeq 4000 instrument.