title: GSM9117298: Microglia R1; Mus musculus; RNA-Seq
library_construction_protocol: Qiazol lysis followed by Qiagen miRNAeasy protocol. Novogene contructed the library. The following is provided by Novogene in relation to library constructoin: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using  dTTP.  After end repair, A-tailing, adapter ligation, size selection, amplification, and purification, the library was ready for sequencing.