title: GSM8521513: 12 adult Ghcre-YFP pituitary glands pooled, FACS sorted; Mus musculus; ncRNA-Seq
library_construction_protocol: Pitutary glands were extracted and dispersed using collagenase.  Dispersed cells were FACS sorted and the GFP positive fraction was collected for RNA extraction using the QIAGEN Rneasy MicroRNA kit. 250ng of RNA was used as input to extract cDNA using a ribo-depeletion method to capture both mRNA and lncRNA