title: GSM9222982: PEA 3; Mus musculus; RNA-Seq
library_construction_protocol: Following a 24-hour treatment with PEA or vehicle control, terminally differentiated C2C12 monolayers were lysed with buffer RLT from the Qiagen RNeasy kit (Qiagen Ltd). RNA was then extracted from cell lysates using the RNeasy kit with proteinase K digestion, as per the manufacturer's guidelines. Eluted RNA was stored at -80oC until required for determination of total RNA and library processing. Libraries were constructed from 100 ng of total RNA with Poly-A tail enrichment of mRNA using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® with Agencourt AMPureXP Sample Purification Beads (Beckman Coulter. Wycombe, UK) as per manufacturers guidelines, by Bart's and the London Genome Centre at Queen Mary, University of London.