title: Titan_Floralbuds_Replicate_3
design: Floral buds sample from each blueberry plant of titan cultivar was used to extract total RNA using Spectrum Plant Total RNA Extraction kit (Sigma-Aldrich, St. Louis, MO, USA). The RNA was treated with Turbo DNA-free kit, and concentration and integrity were quantified using a NanoDrop Microvolume UV-Vis Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. After end repair, A-tailing, adapter ligation, size selection, amplification, and purification, quantified libraries were pooled and sequenced using sequencing-by-synthesis on Illumina Novaseq 6000 platform, producing 150-bp paired-end (PE) reads.