title: GSM9442800: DLM8 untreated - 1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were washed with PBS, then lysed in plate using the RNeasy  RLT buffer with 2-mercaptoethanol.  The lysate was then applied to QIAshredder before completing the RNeasy protocol with DNase digestion as per manufacturer's recommendations. The mRNA-seq libraries were generated from 200 ng of total RNA using the Universal Plus™ mRNA-Seq with NuQuant® kit following the manufacturer's instructions. Briefly, RNA underwent poly(A) selection, fragmentation, cDNA synthesis, end repair, adapter ligation with Unique Dual Indexes (UDIs), and PCR amplification, yielding Illumina-ready libraries