title: metagenomic Human pegivirus
design: viral nucleic acids were extracted by using the DSP Virus Spin Kit (Qiagen, Hilden, Germany) and a modified Sequence-Independent Single-Primer Amplification (SISPA) method was used to enrich for the viral genomes [12]. Library was prepared using Illumina DNA Prep kit and Illumina Nextera DNA CD Indexes, according to the manufactures protocol. Shotgun metagenomic NGS was then performed using the Illumina NextSeqTM 550 platform