title: GSM9221640: primary cardiomyocyte, BMP2+DM, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from primary cardiomyocyte using TRIzol reagent following the manufacturer's instructions. RNA integrity was assessed using the Agilent 2100 Bioanalyzer. RNA library for RNA-seq was prepared as follows: mRNA was purified from total RNA using polyT and then fragmented into 300~350 bp fragments, the first strand cDNA was reverse-transcribed using fragmented RNA and dNTPs (dATP, dTTP, dCTP and dGTP) and second strand cDNA synthesis was subsequently performed. Remaining overhangs of double-strand cDNA were converted into blunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNA fragments, sequencing adaptors were ligated to the cDNA and the library fragments were purified. The template was enriched by PCR, and the PCR product was purified to obtain the final library. After library preparation and pooling of different samples, the samples were subjected for NGS platform by Berry Genomics Co., Ltd (Beijing, China).