title: Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting cold tolerance
design: Transcriptome profiling reveals insight into the cold response of perennial ryegrass genotypes with contrasting cold tolerance
library_construction_protocol: After 21 of growth in peat plants were moved to 50 ml tubes filled with 5 ml water. The tubes were moved to the climate chamber. the plant material were samples within 1 hour after reaching the desired temperature except for the control samples  The plants selected for freezing tests were vegetatively propagated into 20 replicates each with 2-3 ramets. These plants were grown for 21 days in cell packs filled with peat. RNA extraction was carried out using Qiagen RNeasy Plant Mini kit The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)