title: GSM8974377: Anti-PD1-2_scRNA; Mus musculus; RNA-Seq
library_construction_protocol: The lung cancers tissues were collected from Anti-PD1 (n=2) and Anti-PD1+GSK (n=2) SCLC groups and washed with PBS supplemented with 2% fetal bovine serum (FBS). The lung tumors were digested by Collagenase I and IV into single cells and filtered through a 100um filter to obtain a single-cell suspension. Cell numbers were counted and the concentration of single-cell suspension was about 1000 cells/μl. The cell activity of single-cell suspension was more than 85%. Machine and cDNA libraries were amplified according to the operating instructions of 10×Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (1000268). The constructed library was sequenced using the Illumina Nova 6000 platform in PE150 sequencing platform.