title: RNA-seq of human fetal brain cortex
design: RNA-seq of human fetal brain cortex
library_construction_protocol: Human embryonic and fetal tissue samples used for bulk-RNA seq were obtained with ethical approval and informed consent from the Human Developmental Biology Resource (http://www.hdbr.org).The age of embryos up to 8wpc was calculated based on Carnegie staging, whereas in older fetuses the age was estimated from foot length and knee-heel length in relation to standard growth data. Samples were karyotyped by G-banding or quantitative PCR (chromosomes 13, 16, 18, 21, 22 and X and Y) to determine the sex of the embryo/fetus and to exclude any major chromosomal rearrangements or aneuploidies. Total RNA was extracted from human fetal brain cortex samples using the AllPrep DNA/RNA Mini Kit (Qiagen) cDNA libraries were prepared using the KAPA mRNA HyperPrep Kit (Roche)