title: 16S rRNA V3-V4 amplicon sequencing of sediment metagenome: S12
design: Bacterial 16S rRNA V3-V4 amplicons were generated from agricultural drainage sediment collected in Alar, Xinjiang, China, in March 2024. DNA was extracted using a modified CTAB method. The V3-V4 region was amplified with primers 338F and 806R and sequenced on the Illumina NovaSeq 6000 platform with paired-end 250 bp reads.