title: RNA-Seq of Perkinsus olseni: trophozoite
design: Total RNA for was extracted using Trizol reagent (Invitrogen) following manufacturers' instructions and quantitated using the Qubit 2.0 Fluorometer. The strand specific RNA libraries were made using the TruSeq Stranded mRNA Sample Prep Kit according to the manufacturer's instructions. The amplified libraries were sequenced in an Illumina HiSeq 4000