title: GSM8473072: Ishikawa, Input, PAX2 over expression; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin Immunoprecipitation was performed by crosslinking cells and tissues in 1% formaldehyde for 8 minutes, following by quenching in 1.25M Glycine. Lysis and enrichment were carried out using the HighCell ChIP kit Protein G following manufacturer's instructions (Diagenode). Next Generation Sequencing library preparation was carried out using KAPA Hyper Prep kit (KAPA Biosystems) and TruSeq adapters (Integrated DNA Technologies). A quality check of the purified libraries was carried out using the 4200 TapeStation(Agilent).