title: GSM8491407: Liver sample 66; Homo sapiens; RNA-Seq
library_construction_protocol: RNA of the tissue homogenates was extracted by Trizol/Chloroform phase separation technique, then RNA purification was carried out with Qiagen RNeasy mini kit for explant liver samples (Catalog No.74104) and RNeasy micro kit (Catalog No.74004) for biopsy samples. The RNA integrity number (RIN) value of total RNA samples was measured with TapeStation (Agilent Tech, USA). The amount of total RNA was measured more precisely fluorometrically with the RNA Broad Range kit (Thermofisher, USA) by using Qubit (Invitrogen, USA). RNA sequencing library was prepared with Illumina Stranded Total RNA Prep.