title: GSM8490184: WT, 0.4MPa, timepoint 1, RNA-Seq 2, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were lysed directly on cell culture plates in Buffer RLT Plus (Qiagen). The lysate was homogenized using Qiashredder columns (Qiagen) and stored at -80°C. Once all samples were collected, RNA was isolated using a RNeasy mini kit (Qiagen). Quality and concentration of RNA were determined using a 2100 Bioanalyzer Instrument (Agilent). cDNA libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina polyA mRNA workflow (New England Biolabs). Samples were sequenced on a NovaSeq 6000 (Illumina).