title: GSM9117769: brain cells, MCAO,PBS_treated, 14D, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Young (8-10 week-old) transgenic and wild-type male mice on a C57BL/6 background were randomly distributed into PBS group (n=2) and IL2-JES6-1 treated group (n=2). Beginning at 6 hours following MCAO surgery, 7 injections from 6 hours to 14 days after the surgery were intraperitoneally injected into the IL2-JES6-1 treated group, while PBS were intraperitoneally injected into the PBS group. At day 14 after the surgery, cells were dislocated from the ischemic hemisphere of all the mice and sorted for CD45high immune cells using FACS. Immune cells were collected for scRNA-seq. Young (8-10 week-old) transgenic and wild-type male mice on a C57BL/6 background were randomly distributed into PBS group (n=2) and ILC2s-transferred group (n=2). At 6 hours following MCAO surgery, 1*10^6 ILC2s were intravenously transferred into the ILC2s-transferred group, while PBS were intravenously injected into the PBS group. At day 14 after the surgery, cells were dislocated from the ischemic hemisphere of all the mice and were collected for scRNA-seq. The cell suspension was loaded into Chromium microfluidic chips with 3' chemistry and barcoded with a 10× Chromium Controller (10X Genomics). RNA from the barcoded cells was subsequently reverse-transcribed and sequencing libraries constructed with reagents from a Chromium Single Cell 3' reagent kit (10X Genomics) according to the manufacturer's instructions. Sequencing was performed with Illumina (NovaSeq 6000) according to the manufacturer's instructions (Illumina).