title: Fecal metagenomes of Mus musculus female old
design: Metagenomic DNA libraries were generated using a high-quality genomic DNA and libraries were sequenced on the Illumina NovaSeq 6000 PE150 platform with a 250-bp paired-end model. Briefly, samples were subjected to quality control by using Qubit  dsDNA Assay Kit in Qubit  2.0 Flurometer (Life Technologies, USA). 1 g of DNA samples were fragmented to 350 bp by sonication, end-polished, A-tailed, and ligated with the full-length adaptor for Illumina sequencing, followed by PCR amplification. DNA libraries were analyzed for integrity and purity using the Agilent2100 Bioanalyzer (Agilent, USA) and quantified via real-time PCR. The libraries were sequenced on an Illumina NovaSeq platform (Novogene, UK) and 250 bp paired-end reads were generated.