title: GSM9442923: 3384796_WT_rTreg_NO1; Mus musculus; ATAC-seq
library_construction_protocol: Following treatment, cells were washed with 1x cold PBS. Then, 300 µl of Lysis Buffer (10 mM Tris-HCl (pH 7.4), 10 mM NaCl, 3 mM MgCl2, 0.1% MP-40, Molecular Grade H2O) was added to wells and centrifuged at 1,200 g for 10 mins in a swinging bucket centrifuge. Following removal of supernatant containing lysis buffer and cell debris, the nuclei pellet was then resuspended in 50 µl of tagmentation reaction mix containing Molecular Grade H2O, 2x Tagment DNA Buffer (Illumina), followed by the addition of 2.5 µl TDE1 (Illumina) and incubated at 37°C for 0.5 hr. Following tagmentation, libraries were purified using the Qiagen Minelute PCR purification kit (Qiagen) and amplified by PCR. To remove primer dimers, libraries were bead purified using one round of 1.0X Ampure XP beads with 80% ethanol, followed by a second round of 0.6X Ampure beads with 80% ethanol. Libraries were sequenced (50-bp paired end reads).