title: Identification of binding sites of the ERF019 gene in Brassica napus using DAP-seq
design: Genomic DNA was sheared into fragments ranging from 100 to 400 bp using a Covaris M220. These fragments were then used to construct libraries with the mich TLX DNA-seq kit. The coding sequence of TF was cloned into the pFN19K HaloTag T7 SP6 flexi expression vector, and the resulting halo-tagged fusion protein was induced and subsequently purified. This fusion protein was then co-incubated with the genomic DNA, followed by the isolation of TF-bound DNA sequences. The assay included two replicates (TF-1 iand TF-2) and one negative control(TF-input).