title: GSM8953347: knock out; Mus musculus; RNA-Seq
library_construction_protocol: For single cell RNA analysis of pulmonary dendritic cells and their precursors, lungs of 3 individual Csf2ra+/– or Csf2ra–/– mice, neonatally reconstituted with AM, were homogenized as described above and sorted using a FACSymphony S6 (BD Bioscience). After debris and doublet exclusion, live CD45+CD3–CD19–Ly6G–SiglecF–CD11c+ cells were sorted into PBS containing 0.05% BSA for the 10X Genomics protocol. Sorted cells were loaded into 10X Genomics Chromium. Libraries were prepared following the manufacturer's protocol (Chromium Next GEM Single Cell 3' Reagent Kits v3.1 protocol)