title: GSM8800685: Prrx1-Cre E13.5 biol rep 1; Mus musculus; RNA-Seq
library_construction_protocol: At E13.5, hindlimbs were harvested in ice cold PBS and the paws were removed. The samples were digested in 0.25% trypsin-EDTA for 1hr at 37 oC. For E15.5, knees were dissected in ice cold pbs and digested in 3 mg/ml collagenase D in DMEM for 45 minutes at 37 oC. They were then digested in 1 mg/ml collagenase D in DMEM for 3 hours at 37 oC. Following digestion, samples from both timepoints were gently dissociated by pipetting, filtered through a 40 μm strainer, and centrifuged for 10 minutes at 400 g. The cells resuspended in 0.4% FBS in PBS at a concentration of 1000/μL. Samples (maximum 10,000 cells per sample) were loaded on the 10X Genomics Chromium platform, barcoded, and sequenced on the Illumina NovaSeq SP flowcell platform at a depth of 500 million reads per sample.