title: CRISPRi screen on MG1655 and LF82 in the gut of DSS-induced colitis mouse model
design: After introducing E. coli harboring a CRISPRi plasmid library into the mouse gut via oral gavage, plasmid DNA was extracted from mouse stool, and amplicon sequencing of the sgRNA region was performed to evaluate the relative abundance of each sgRNA within the E. coli population in the gut.