title: RRBS of mus musculus: GF mucosa
design: DNA and RNA were simultaneously isolated from each tumor and adjacent off-tumor sample using the AllPrep DNA/RNA Mini kit (QIAGEN). Briefly, frozen samples were homogenized in 350 L of RNA lysis buffer (RLT) buffer containing 10L/mL of -ME, in a sterile Eppendorf tube, using an electric pestle and subsequently passed through a 20-gauge needle fitted to an RNAse-free syringe 10 times. The homogenate was then centrifuged at maximum speed for 3 minutes in a tabletop centrifuge and processed according to the standard kit instructions. Isolated DNA was eluted in 100 L of elution buffer, and isolated RNA was eluted in 50 L of RNAse-free water.