title: GSM9118453: DIN cells 14 days untreated (R882H on) rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in Trizol reagent (Thermo Fisher) and RNA was extracted using the Direct-zol RNA Miniprep kit (Zymo Research). RNAseq was performed at the Peter MacCallum Cancer Centre Molecular Genomics core facility. RNAseq libraries were generated using the QuantSeq 3¢ mRNA-seq Library Prep Kit for Illumina (Lexogen) and sequenced on an Illumina NextSeq500 to produce 75-100 bp single-end reads with a depth of 6-10x106 reads per sample.