title: RNA-seq of Carpinus putoensis: leaf
design: High-throughput RNA sequencing (RNA-Seq) was conducted using Illumina sequencing technology, with an average sequencing depth of more than 50, and saturation tests were performed to ensure comprehensive coverage. cDNA libraries were constructed from the pooled RNA samples, and sequencing was performed with paired-end reads. The sequencing depth was carefully controlled to ensure comprehensive coverage of the transcriptome