title: GSM9003629: RAW264.7cells Blank-3; Mus musculus; RNA-Seq
library_construction_protocol: mRNA was enriched from total RNA using oligomer (dT) magnetic beads. The mRNA fragments were cut into short fragments of about 300bp using a cleavage buffer. The first cDNA was synthesized by using mRNA as template and random hexamer as primer. The purified double-stranded cDNA was subjected to terminal repair, A-tail modification and sequencing joint connection. Select fragment size using AMPure XP beads. PCR amplification was performed to obtain the final cDNA library.