title: GSM8835880: DFCI393_331; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using RNeasy mini kit (Qiagen #74106) including a DNase digestion step according to the manufacturer's instructions and RNA sequencing was performed. The libraries for RNA-seq are prepared using NEBNext Ultra II non-stranded kit.  Paired end 150bp sequencing was performed on Novaseq6000 sequencer using S4 flow cell. Sequencing reads were mapped to the mm10 genome by STAR. Statistics for differentially expressed genes were calculated by DESeq2.