title: RNA-Seq of brittle star: rock sample P6
design: the full length 16s rRNA gene was amplified from the extracted gDNA samples with PCR using universal primers 27F (AGAGTTTGATCMTGGCTCAG) and 1492R (CGGTTACCTTGTTACGACTT). The PCR machine was run as follows: 95 C for 2 minutes, followed by 30 seconds at 95 C for 27 cycles, 30 seconds at 55 C, and 60 seconds for 72 C and a final extension for 5 minutes at 72 C.