title: unclassified sequence intact rock glacier metagenome_madritsch_September 02
design: otal community DNA was quantified using the Qubit HS dsDNA Kit.Libraries were prepared using the Illumina Nextera XT DNA Library Prep Kit according to the manufacturers protocol.The method is based on random enzymatic fragmentation (tagmentation) and adapter ligation.Dual indices were added using the Nextera XT Index Kit for sample multiplexing.Libraries were sequenced on an Illumina MiSeq platform using a MiSeq Reagent Kit with 2 x 150 bp paired-end reads.Approximately 100,000 clusters were generated per sample.Sequencing output was provided as demultiplexed paired-end FASTQ files (sample_R1.fastq.gz and sample_R2.fastq.gz).