title: scRNAseq of PbT-II nTregs
design: Single-cell RNA-seq libraries were prepared using the Illumina 3' single cell RNA prep protocol (T20 kit) from flow-sorted PbT-II nTregs. Libraries were constructed per the manufacturer's protocol (Document # 200063183 v01, August 2025( and sequenced on an Illumina NovaSeqX.