title: 16S rRNA amplicon sequencing of mouse colon contents - LPS-4
design: 16S rRNA gene amplicon sequencing of bacterial community DNA extracted from mouse colon contents. The V3-V4 hypervariable region was amplified using primers 338F (5'-ACTCCTACGGGAGGCAGCAG-3') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). Mice were male C57BL/6, 7 weeks old. Sample LPS-4 belongs to the LPS group: LPS-induced acute lung injury mice receiving normal drinking water. Libraries were prepared using the NEXTFLEX Rapid DNA-Seq Kit and sequenced on an Illumina NextSeq 2000 platform.