title: GSM8734748: RH30, vehicle (DMSO, 48 h), rep2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was isolated using TRIzol (Thermo Fisher Scientific, 15596018) according to the manufacturer's instructions. After RiboGreen quantification and quality control by Agilent BioAnalyzer, 500 ng of total RNA with RIN values of 9.3-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR.