title: RNA-seq of A549 OAS2 KO and KI cells infected with EMCV
design: RNA-seq of A549 OAS2 KO and KI cells infected with EMCV
library_construction_protocol: Cells were seeded at a density 6250 cells/well in 1ml DMEM, FBS in 12 well plate. The next day cells were stimulated with 1ug/ml doxycycline for 3days. Afterwards cells were infected with EMCV MOI 0.3 or treated with media alone for 24h until cell harvest.  Cells were washed twice with PBS and lysed in the well with LBP buffer and RNA isolated following manufacturers instructions from Macherey Nagel NucleoSpin RNA Plus RNA isolation kit with gDNA removal. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.