title: GSM9221404: A549-sh-circFUT8-3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using TRIZOL reagent (Invitrogen, Grand Island, NY) and further purified with the Qiagen RNeasy Mini Kit (Qiagen, Valencia, CA). RNA integrity was assessed using an Agilent BioAnalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA), and samples with an RNA Integrity Number (RIN) greater than 7.0 were used for subsequent library preparation. The TruSeq RNA Sample Preparation Kit (Illumina) was employed for mRNA purification, fragmentation, first-strand cDNA synthesis and second-strand cDNA synthesis.The resulting libraries were quantified using a Qubit fluorometer (Invitrogen, CA, USA), pooled in equimolar amounts, and sequenced on an Illumina NovaSeq 6000 platform.