title: GSM8491571: SCS with drought treatment (1); Sorghum bicolor; RNA-Seq
library_construction_protocol: Total RNA was extracted from samples using TRIzol reagent. RNA Nano 6000 Assay Kit and Bioanalyzer 2100 system (Agilent Technologies, CA, USA) were used to measure RNA concentration and detect RNA integrity. The cDNA library was sequenced on the Illumina NovaSeq 6000 by Novogene Co., Ltd. Strand-specific libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.