title: Barisia UCE phylogeny
design: We extracted genomic DNA from tissue samples using Qiagen DNeasy Blood & Tissue Kits (Qiagen Inc., Valencia, CA, USA). Aliquots of extracts were shipped to RAPiD Genomics (Gainsville, FL, USA) for UCE library preparation and sequencing. There, 5472 custom-designed probes (MYbaits; MYcroarray, Inc., Ann Arbor, MI, USA) were used to enrich 5060 UCE loci using standard enrichment protocols (Faircloth et al., 2012). Libraries were sequenced on an Illumina HiSeq 3000 (100 paired-end) at the University of Florida Interdisciplinary Center for Biotechnology Research Facility