title: metatranscriptome of Lake Washington sediment enrichment, replicate 3
design: DNA samples were prepared using NEBNext Ultra II DNA Library Prep Kit with a 450-bp mean insert size. Total RNA samples were hybridized with NEBNext rRNA Depletion Solution to diminish rRNA from the samples substantially, and sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina.