title: Pabcio Isoform Sequencing
design: RNA was extracted from leaf tissue using the SMARTer PCR cDNA Synthesis Kit (Clontech, Catalog No. 634925), and approximately 1 g of total RNA was used for library construction. The SMRTbell library was then generated using the SMRTbell Template Prep Kit 1.0-SPv3 and sequenced on the Sequel system (Pacific Biosciences).