title: GSM8800061: Patient6_MicrocysticTissue6B; Homo sapiens; RNA-Seq
library_construction_protocol: Following RNA extraction using the RNeasy Plus Mini kit (Qiagen) according to the manufacturer's protocol, RNA concentration and quality were determined using DropSense96 (Perkin Elemer) and Bioanalyzer (Agilent). For RNA-Seq library preparation, the NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina (Bioké) was used according to the manufacturer's protocol. After quality assessment of the obtained libraries, an equimolar pool was prepared. A qPCR was then carried out using the Kapa SYBR FAST Universal qPCR kit for Illumina (Roche).