title: MiSeq of fungi: Glechoma longituba
design: The E.Z.N.A.  soil DNA Kit (Omega Biotek, Norcross, GA, U.S.) was used to extract DNA from the surface sterilized above and below ground, as well as from the phyllosphere, rhizosphere, and periroot soil. The E.Z.N.A.  soil DNA kit comes with detailed instructions to guide researchers through the DNA extraction process. The 18S r RNA gene was amplified by PCR using the fungal primer ITS1F/ITS2R (Li et al., 2023). The bacterial primer 799F / 1392R was first used (Bulgarelli et al., 2012; Lundberg et al., 2012) amplified the 16S r RNA gene and then amplified the 16S r RNA gene with primer 799F / 1193R (Bulgarelli et al., 2015). The purified amplicon was run on the Illumina nextseq platform (2  387bp).