title: GSM9034695: KOA-2; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer protocol. Sequencing libraries were prepared from 500ng total RNA using TruSeq stranded total RNA library preparation kit with ribosomal depletion using RiboZero Gold (Illumina Inc.) and samples were paired-end sequenced (150 cycles in one NovaSeq 6000 SP flowcell).