title: NextSeq 500 sequencing of SAMD00098238
library_construction_protocol: Monosomes were purified by ultra-centrifugation (Beckman coulter, Optima MAX-TL Ultracentrifuge) through 50% sucrose. Next, ribosome pellets were digested with Proteinase K. Finally, the ribosome protected fragments (RPFs) were size-selected (25-35 nt) by polyacrylamide gel electrophoresis, and converted to Illumina sequencing library using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs).