title: RNA-Seq of carambola:long morph pistils at third stage.
design: NEB Standard Library Construction: Using fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA is synthesized in the M-MuLV reverse transcriptase system. Subsequently, the RNA strand is degraded by RNaseH, and the second strand of cDNA is synthesized using dNTPs as substrates in the DNA polymerase I system. The purified double-stranded cDNA undergoes end repair, addition of A tails, and ligation of sequencing adapters. AMPure XP beads are used to select cDNA fragments of approximately 250-326 bp, which are then amplified by PCR and purified again using AMPure XP beads to obtain the final library. The kit used for library construction is the NEBNext Ultra RNA Library Prep Kit for Illumina.