title: RNA-Seq data from the TBAIT Kenya study examining clinical and transcriptomic characteristics associated with Tuberculosis cough aerosolization.
design: RNA was isolated using PAXgene miRNA spin columns (Qiagen, Part Number 767134) and globin reduction was performed using GlobinClear Human (ThermoFisher, Part Number AM1980). Isolated RNA (0.5 ng) was added to reaction buffer from the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara, Part Number 634891), and reverse transcription was performed followed by PCR amplification to generate full length amplified cDNA. Sequencing libraries were constructed using the NexteraXT DNA sample preparation kit (Illumina, Part Number FC-131-1096) to generate Illumina-compatible barcoded libraries, which were pooled and quantified using a Qubit® Fluorometer (Life Technologies). Sequencing was performed on the Illumina NextSeq 2000 platform, with paired-end 59-base reads, using a NextSeq P3 sequencing kit (Illumina, Part Number 20100989) with a target depth of 5 million reads per sample. Base calls were processed to FASTQs on BaseSpace (Illumina), and a base call quality-trimming step was applied to remove low-confidence base calls from the ends of reads.