title: GSM8799193: WT24hr_rep2; Mus musculus; ATAC-seq
library_construction_protocol: The ATAC (mt-remove) protocol was adapted from previously established methods8,9 with modifications. A total of 10,000 cells were collected, washed with 1 mL of ice-cold PBS containing EDTA-free protease inhibitor, and centrifuged at 500 × g for 5 minutes at 4 ℃. The supernatant was completely removed, and the cell pellet was resuspended in 20 μL of transposase reaction mixture (homemade), consisting of 1× TD buffer, 1 μL Tn5 enzyme, and 0.02% digitonin. The transposition reaction was carried out at 37 ℃ for 30 minutes with continuous agitation. Following transposition, DNA was purified and the ATAC-seq library was constructed using the KAPA HyperPrep Kit (KAPA Biosystems, 07962363001) and barcoded with NEXTflex DNA barcodes (Integrated DNA Technologies).