title: GSM9117923: zebrafish, head removed, phox2bb+, 3dpf; Danio rerio; ATAC-seq
library_construction_protocol: About 150 embryos were dissected and separately stored in 6 low-binding microcentrifuge tubes on ice. After dissection, 400 μL Accumax (Invitrogen, Catalog number: 00-4666-56) solution was added into each tube. The tubes were incubated in the Eppendorf ThermoMixer at 30 °C and shaken at 1,000 rpm for about 25 min for cell dissociation. After the dissociation step, the tubes were chilled on ice, and 1 mL of cold HBSS-BSA solution was added to quench the reaction. The solution was passed through a 70 μm cell strainer into a 50 mL conical tube, and an additional 500 μL HBSS-BSA solution was used to rinse the tube and subsequently collected into the conical tube. The solution was then spun down at 4 °C at 1,200 rpm for 11 min. The supernatant was carefully removed and the pellet in each conical tube was resuspended in 1 mL HBSS-BSA solution. We then performed fluorescence-activated cell sorting and sorted out Kaede-positive and Kaede-negative cells. Around 10, 000 Kaede-positive cells and 50,000 Kaedae-negative cells were sorted. Sorted cells underwent cell lysis, and Tn5 tagmentation. The library was constructed by amplifying the tagmentated DNA with N50x, N70x index, and then purified with magnetic beads. The procedue followed Omni-ATAC protocol.