title: 16S rRNA full-length amplicon sequencing of Quseir rhizosphere sediment
design: Full-length 16S rRNA (~1500 bp) amplicons were generated using the Oxford Nanopore 16S Barcoding Kit (124). Genomic DNA was tagmented and barcoded using 27F/1492R primers with 5 tags, followed by AMPure XP bead cleanup and Qubit quantification. Normalized libraries were pooled and sequenced on a MinION Mk1C. Reads were processed using FastQC, Porechop_ABI, and NanoFilt, followed by taxonomic assignment through the Epi2me 16S workflow (minimap2, samtools, taxonkit). Downstream analysis was performed using R, phyloseq, microbiome, ggplot2, and ComplexHeatmap.