design: ALFA-SEQDNALibraryPrepKit was used for library construction with steps as follows:  (1) DNA fragmentation; (2) Endrepair and 3' adenylate addition; (3) Adaptor ligation, fragment selection, and purification .The size distribution of the libraries was evaluated on the Qsep400 High-Throughput Nucleic Acid-Protein Analysis System (Hangzhou Houze Biological Technology Co., Ltd., China), while the library concentrations were measured using the Qubit 4.0 (Thermo Fisher Scientific, Waltham, USA); (5) Sequencing.