design: Paired-end libraries (2x50 bp) were constructed using the Universal RNA-Seq with NuQuant kit (Nugen Tecan Genomics) from 250 or 45 ng of total RNA. Ae. aegypti ribosomal RNA was depleted using a custom AnyDeplete probe mix (Nugen Tecan Genomics). After quality check, libraries were pooled in equimolar concentrations and sequenced.