library_construction_protocol: Pituitaries were dissected from e11 embryos (n=246), trypsin-dispersed, and pooled. Cells were counted, and the cell viability was assessed by the trypan blue dye-exclusion method. An individual e11 pituitary gland yielded around 300,000 cells and cell viability was > 95% in all experiments. Dispersed cells were plated at a density of 1,000,000 cells per well in poly-L-lysine-coated 24-well plates and were allowed to attach for 1 h at 37.5°C. Dulbecco's Modified Eagle Medium w/F12 modifications (DMEM/F12) medium supplemented with 0.1% bovine serum albumin (BSA), 100 U/mL penicillin, and 100 μg/mL streptomycin was added, and the cells were cultured for an additional 19 h. CORT (100 nM final concentration) was added to appropriate wells for an additional 6 h, and the cells were fixed following the methanol fixation and rehydration of the single cell suspension according to GENEWIZ protocol (10x Genomics). Methanol fixed cells were further processed by the Next-Generation Sequencing team at GENEWIZ from Azenta Life Sciences. At GENEWIZ, cell viability was assessed and, if necessary, dead cell removal was performed. Cells were then processed by the 10x Genomics Chromium™ Controller to create single-cell libraries. Within the Chromium Controller, cells were loaded onto a microfluidic chip and encapsulated within droplets containing barcoded gel beads and reagents for reverse transcription. Following cell lysis, the beads captured the poly(A) tails of the mRNA molecules. Reverse transcription generated cDNA tagged with a 10x barcode to identify the cell and a unique molecular identifier (UMI) to label the mRNA transcript. The pooled cDNA was amplified in bulk, and Illumina adapters, including a sample index, were added to the fragments to generate sequencing libraries. The libraries were then pooled and sequenced on the Illumina® platform.