library_construction_protocol: After being treated with 15μM thioridazine for 24h, the SKOV3 cells were lysed with Trizol (Invitrogen, USA) and total RNA was extracted using Trizol reagentaccording to the manufacturer's instructions.RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA). A total amount of 3 μg RNA per sample was used as input material for the RNA sample preparations. Firstly,ribosomal RNA was removed by Epicentre Ribo-zero™ rRNA Removal Kit (Epicentre, USA), and rRNA free residue was cleaned up by ethanol precipitation. Subsequently, sequencing libraries were generated using the rRNA-depleted RNA by NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations.