library_construction_protocol: Twenty-four hours post-transfection, RNA was extracted and purified from DF1 cells using RNeasy Plus Mini Kit (Qiagen) and the integrity of the RNA was measured using a 2100 Bioanalyser (Agilent). The RNA was sent to the Australian Genome Research Facility, Melbourne, Australia (AGRF) for RNA sequencing with n=3 samples per group. Poly-A enrichment was carried out to eliminate ribosomal RNA.