design: RNA sequencing was performed on renal tissues from C57BL/6 mice exposed to acute carbon monoxide poisoning (ACOP) to investigate gene expression changes associated with renal injury. Total RNA was extracted, and libraries were prepared using the NEBNext Ultra RNA Library Prep Kit. Sequencing was performed on an Illumina platform, and data were analyzed to identify differentially expressed genes and signaling pathways involved in inflammation and apoptosis.