design: RNA was extracted from the cell lysates as per the manufacturers instruction supplied with the TRIzol reagent. The extracted RNA was purified using a Qiagens RNeasy clean-up kit (catalog No: 74104) and RNA integrity was assessed on an Agilent TapeStation using standard RNA screen tape. RNA (1 g) with a RIN score > 8 was used to prepare poly-A enriched RNA libraries following the manufacturers protocol for the Illumina stranded mRNA prep kit (catalog no.: 20040534). Libraries for different samples were barcoded with Illumina index primers provided in Illumina RNA UD Indexes Set A. The quality of each library with respect to size, distribution, and yield was assessed using Agilent TapeStation DNA 1000 screen tape. RNA sequencing libraries were pooled in equimolar ratios and sequenced on Illumina Novaseq X 300 (150 bp paired end) platform at AGRF.