design: HMW DNA was extracted from a whole, male Aedes togoi pupa using a modified version of the NEB Protocol for High Molecular Weight (HMW) DNA Extraction from Tissue, utilizing changes to reagent volumes outlined in the Using Very Low Tissue Amounts section of the T3060 kit. Library preparation was conducted using the PacBio ultra-low DNA input workflow. HiFi sequencing was carried out on two SMRT PacBio Sequel II flow-cells.