library_construction_protocol: Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturer's protocol. cDNA library preparation and bulk RNA sequencing were conducted by Novogene America (Sacramento, CA, USA) using the Illumina HiSeq™ X Ten platform. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library.