library_construction_protocol: RNA was extracted using Rneasy mini kit(Qiagen), all samples normalized to 50ng total concentration in 11ul for input to library preparation Samples were then prepared using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (#  E7420) according to manufactures protocol, barcoded using NEBNext® Multiplex Oligos for Illumina (NEB #E6440S/L)