design: Genomic DNA was extracted from leaves of a high THC cannabis cultivar and used to construct the DNA library. The CDS of candidate TFs were cloned into the pH6HTN His6HaloTag T7 vector and the HALO-tagged TFs were expressed in vitro using the wheat germ protein expression system. The expressed HALO-TF proteins were bound to magne HaloTag beads and were used for binding to DNA, followed by PCR enrichment, indexing and pooling of individual libraries for sequencing.