design: Single-cell ATAC sequencing of kidney marrow cells from heterozygous gata2b mutated and wild type zebrafish
library_construction_protocol: Adult zebrafish were euthanized, kidney marrow was isolated and dissociated by pipetting in PBS/10% FCS. 7-AAD (7-amino-actinomycin D) 0.5mg/L (BD biosciences) was used for live/dead discrimination. Sorting was performed using FACSAriaIII (BD). Single viable cells were sorted from 3 pooled kidney marrow of female Tg(CD41:GFP) zebrafish and supplemented with 114-1607 CD41:GFPlow expressing cells. Chromium Single Cell ATAC reagent kits, 10x Genomics Chromium Single Cell ATAC reagent kits, 10x Genomics