design: The target region was amplified by PCR and this PCR product served as a template for to additional consectutive PCRs to attach a total of four barcodes (inner_for, inner_rev, outer_for, outer_rev) and Illumina compatible adapter sequences. Barcoded amplicons were pooled in batches of <40 and PCR purified twice to remove primers. Amplicons were sequenced in 2X250bp or 2X300bp mode at Eurofins genomics or Genewiz/Azenta