design: Human male brain samples were obtained from the Department of Pathology and Experimental Cancer Research, Semmelweis University, Budapest, Hungary. Tissue blocks were fixed in sterile PBS containing 4% paraformaldehyde with ATA RNA-protective agent, cryoprotected in 20% sucrose and ATA. Kisspeptin neurons of the mediobasal hypothalamus were identified by immunostaining using a novel ImmunoLCM protocol. Labeled neurons were collected by laser capture microdissection and processed for RNA extraction and RNA-Seq using the Illumina platform.