library_construction_protocol: Animals were sacrificed and Livers were collected, snap frozed in liquid nitrogen.Total RNA isolation from snap-frozen liver tissue or cultured cells was performed with RNeasy Mini Kit (Qiagen, 74106). The on-column DNA digestion was carried out using an RNase-free DNase kit (Qiagen) or RNA samples were treated with TURBO DNase (ThermoFisher Scientific, AM1907) according to the manufacturer's protocol to completely remove genomic DNA.  Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols ( TruSeq Stranded).