design: Primer walking experiment and sequencing for sample B4: Amplicons were assembled individually and produced contigs of expected size (2417bp, 1035bp). The amplicon reads were combined with the initial iSeq and NextSeq data, and then assembled with spades generating 1 contig of 29,691 nt. These amplicons were then added to a KRAKEN2 analysis of the original B4 sample reads to generate a complete viral genome.