design: Genomic DNA was fragmented using a Covaris ultrasonicator (Covaris, USA), and fragments of 200-400 bp were size-selected with the AMP-Medium Kit (Beckman Coulter, USA). A library building technique for linear amplification using T7 RNA polymerase, primarily for RNA/DNA amplification of trace samples. Libraries were quality-checked and sequenced on the DNBSEQ-T7 platform (MGI, China). Raw data were processed with fastp (v0.21.0)  to remove adapters, low-quality reads (Q < 20), and reads with consecutive N bases. Long-read sequencing (>20 kb) was conducted on the PacBio Revio platform.