library_construction_protocol: Total RNA (~30-120 ng) was extracted using RNA Clean & Concentrator-5 (ZYMO R1013) following manufacturer's instruction. mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490L). Libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB E7760L) and following manufacturer's instructions.