library_construction_protocol: RNA was extracted from groups of Control or DjTcf4 RNAi animals using standard Trizol isolation. The mRNA was captured using Oligo-dT beads. The mRNA was reverse transcribed to synthesize cDNA that was modified by end repairing, A-tailing and adapter ligation. The NGSTM DNA Selection Beads were used to purify target fragments. PCR library amplification was then performed, and sequencing was carried out using the Illumina Novaseq X Plus with a PE150 dual-indexed setup.