design: This sample represents NE91, a transposon insertion mutant of Staphylococcus aureus strain JE2 from the Nebraska transposon mutant library. The mutant is intended to carry a disruption in the kdpA gene. Whole genome sequencing was performed using Illumina paired-end technology to confirm the transposon insertion site. This identified a transposon disruption in the SAUSA300_1785 gene, rather than kdpA.