library_construction_protocol: Total RNA from algal cells was extracted using the RNAprep pure plant kit (Tiangen, China) according to the manufacturer's instructions. The concentration, purity, and integrity of the extracted RNA were determined using the NanoDrop 2000 (Thermo Fisher Scientific, USA) and the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, USA). Sequencing libraries were generated using Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, China), the index codes were added to the sequence of each sample, and the quality of the constructed libraries was assessed by the Agilent Bioanalyzer 2100 system. After the quality of the library was qualified, sequencing was performed using an Illumina NovaSeq 6000 platform.