library_construction_protocol: ChIP was performed with anti-H3K27ac antibody. Briefly, Huh-7 cells stably expressing MCCC2K269R and MCCC2K269Q were cross-linked with 1% formaldehyde for 15 minutes at room temperature and stop cross-link by glycine. Chromatin was fragmented to an average size of approximately 300–500 bp by sonication. Then, fragmented chromatin was incubated with 5μg of anti-H3K27ac antibody and nonspecific rabbit IgG at 4°C overnight. After overnight immunoprecipitation, each sample was washed with washing buffers. The precipitated products were then eluted with elution buffer at 65°C and reversed the cross-link with 5 mol/L NaCl at 65°C overnight. The DNA was finally purified by PCR purification kit (Qiagen). The chipped DNA was ligated with adaptors. After purification, PCR reaction was done with adaptor-ligated DNA and index primer for multiplexing sequencing. Library was purified by using magnetic beads to remove all reaction components. Qualities of libraries were checked by High Sensitivity DNA Assay (Agilent Technologies) and sequenced on an Illumina Nova 6,000 sequencer.