design: Total RNA of body wall, coelomic fluid, tentacle, respiratory tree, male and female gonads, aquapharyngeal complex (intact and regenerative anlage after 15 days post-evisceration), egg, blastula, early and middle gastrula, and pentactula tissues was isolated using the ExtractRNA protocol (BC032, Evrogen). We pooled all extracted total RNA and made a library using a MGIEasy RNA Directional Library Prep kit (1000006386, MGI). Poly-A RNA enrichment was performed with VAHTS mRNA Capture Beads (N403, Vazyme).