library_construction_protocol: chromatin was fixed with disuccinimidyl glutarate (DSG) and formaldehyde. The crosslinked chromatin was then digested in situ with micrococcal nuclease (MNase). Following digestion, the cells were lysed with SDS to extract the chromatin fragments and the chromatin fragments were bound to chromatin capture beads. Next, the chromatin ends were repaired and ligated to a biotinylated bridge adapter followed by proximity ligation of adapter-containing ends. After proximity ligation, the crosslinks were reversed, the associated proteins were degraded, and the DNA was purified Purified DNA was used as input for sequencing library preparation using Illumina-adapters. Biotin-containing fragments were isolated using streptavidin beads prior to PCR amplification. The library was then enriched for promoter regions using Dovetail® Pan Promoter Enrichment Kit (CP3-PP-002) following manufacturer instructions.