library_construction_protocol: Total RNA was extracted using TRIzol 3-5µg of total RNA underwnet zinc-mediated fragmentation into fragments of approximately  200nt. To enrich for RNA fragments containing m6A modifications, magnetic beads were first prepared in IP buffer and 5 μg anti-m6A antibody (Millipore) by mixing for 6 h at 4 ̊C. The antibody-bead mixtures were resuspended in IP reaction solutions containing fragmented total RNA. m6A-enriched fragmented RNA was eluted from the beads using the Rneasy Mini Kit (Qiagen). The eluates were resuspended in ethanol and transferred to an Rneasy MiniElute spin column (Qiagen) and centrifuged at high speed.