library_construction_protocol: PBMCs were isolated from buffy coats provided by EFS. Monocytes were purified by magnetic isolation from PBMCs with the Classical Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) and stained with an anti CD14-PE antibody (Biolegend) for sorting on a BD FACSAria Fusion flow cytometer (BD Biosciences). The library construction protocol was performed according to the manufacturer's instructions (BD Rhapsody Single-Cell Analysis System)