library_construction_protocol: Total RNA was extracted using RNeasy Plus Micro Kits (Qiagen, #74034). RNA integrity was determined via bioanalyzer (Agilent) using standard RNA 6000 Pico chip. The libraries of samples for unstranded sequencing were constrcuted by TruSeq RNA Library Prep Kit and sequenced. Samples for stranded sequencing underwent ribosomal depletion, strand-specific library (dUTP method) synthesis followed by deep sequencing on NovaSeq 6000 platform.