design: For extraction of total RNA, 20-30 mg of feline tissue was added to 600 ul buffer RLT (Qiagen) with 10 ul beta-mercaptoethanol in Lysing Matrix D tubes (MP Biomedicals) and homogenised using a FastPrep-24 Classic bead beating grinder and lysis system (MP Biomedicals). RNA was purified from the resulting homogenate using a RNeasy Plus Mini kit (Qiagen), including column-based elimination of genomic DNA, according to the manufacturers instructions. RNA purity was assessed using a Nanodrop ND-1000 instrument (ThermoFisher), and RNA integrity was determined using a Tapestation 4200 (Agilent). RNA-seq libraries were prepared from total RNA by Oxford Genomics Centre (Wellcome Centre for Human Genetics, University of Oxford). Libraries were prepared following ribodepletion with the NEBNext rRNA Depletion Kit v2 (NEB) to remove abundant rRNA species. Stranded libraries were generated using a TruSeq Stranded Total RNA kit (Illumina) and sequenced as 150bp paired-end reads on a NovaSeq6000 instrument (Illumina) with a target depth of ~100M reads per sample.