library_construction_protocol: 4SU is a derivative of uracil that can be recognized by the RNA polymerase in cells and incorporated into the RNA chain being synthesized. The biotin is chemically labeled on the RNA molecule containing 4SU. The newly synthesized and biotin-labeled RNA is then separated from the total RNA using magnetic beads coated with streptavidin.Extracted twice using Trizol reagent following the instruction. The native RNAs were isolated and examined. After checked the quantity and quality and poly(A) RNA depletion, the TT-seq RNA library were generated using the no coding RNA library method with random primers and sequencing analyzed using Nova PE150 platform.