library_construction_protocol: Cells were trypsinized with 0.25% trypsin-EDTA and resuspended in 1X PBS (calcium and magnesium-free). For the ATAC-seq library preparation, 5×104 viable cells were used and collected. The cell pellets were resuspended in cold lysis buffer (1 M Tris-HCl, pH 7.4, 5 M NaCl, 1 M MgCl2, IGEPALCA-630) and incubated on ice for 5 min. Then, the cells were collected and resuspended with 50 μl transposition mixtures according to ATAC Kit instructions (Vazyme). Mixtures were incubated for the PCR reaction. Mixtures were purified with VAHTS DNA Clean Beads (Vazyme) and amplified for 15 cycles using TruePrep Index Kit V2 for Illumina (Vazyme). Amplification products were further purified with VAHTS DNA Clean Beads.