library_construction_protocol: CUT&Tag was performed using the Hieff NGS® In-Situ DNA Binding Profiling Library Prep Kit for Illumina (Yeasen, 12597ES) according to the manufacturer's instructions. Briefly, 1x105 of cells were used for the CUT&Tag experiment, cells were collected, washed, incubated with concanavalin A-coated magnetic beads, incubated with primary antibodies, secondary antibodies and cutted by pA-Tn5 transposase to generate DNA fragments. CUT&Taglibrary amplification, puried DNA was mixed with 2×HiFi Amplification Mix and uniquely barcoded i5 and i7 primers provided by Hieff NGS® Tagment Index Kit for Illumina® (Yeasen, 12416)following PCR program and purified by DNA Selection Beads.