design: Genomic DNA (gDNA) was extracted from the leaves and bud tip of B. napus. The fragmented gDNA was used to construct libraries using the NGS0602- MICH TLX DNA-Seq Kit. The coding sequence of BnaA9.NST3 was cloned into the expression vector, expressed using the wheat germ extraction system. The purified protein was incubated with the gDNA library. gDNA fragments were eluted from two technical replicates as well as from the library incubated with beads but without added protein as a negative control. Eluted DNA fragments were sequenced on an Illumina NavoSeq. DAP-Seq reads were aligned to the B. napus genome (Darmor-bzh v4.1) using the BWA-MEM.