library_construction_protocol: Cdh5-CreERT2;mT/mG;Restflox/flox mice and Cdh5-CreERT2;mT/mG mice controls were treated  to activate the Cre recombinase at 8 weeks of age and induce atherosclerosis. After 16 weeks of HFD administration, mice were euthanized and their aortas were perfused with PBS. The aortas were flash frozen in liquid nitrogen. Nuclei were isolated from the frozen tissue using a nucleus isolation protocol provided by 10X. Single nucleus RNA-sequencing libraries were prepared using the 10X 3' v4 kit. One mouse per single nucleus capture and 2 captures per group were performed targeting 10,000 cells per capture and sequenced at a read depth of 50,000 reads per cell. All snRNAseq samples were processed and sequenced at the same time as a single batch to minimize batch effects. Single nucleus RNA-sequencing libraries were prepared according to the manufacter's instructions using the 10X 3' v4 kit