library_construction_protocol: Purified RNA was isolated using the miRNeasy Mini Kit (QIAGEN, cat# 217004). A total of 7 ng of RNA was used for library construction. Libraries were prepared for sequencing using the SMARTer Stranded Total RNA-seq Kit v2 (cat# 634413) – Pico Input Mammalian (Takara Bio USA). RNA samples were first converted into cDNA, then adaptors for Illumina sequencing were added through PCR. The PCR products are purified using AMPure Beads (Beckman Coulter, cat# A63881), then ribosomal cDNA was depleted. After depletion, the samples were further amplified using PCR, then purified again using AMPure Beads. Each sample was then qPCR quantified using a KAPA Library Quantification Kit (Roche, cat# 07960140001) and Phix Control Kit V3 (Illumina, cat# FC-110-3001). Samples were then pooled together and quantified again using the KAPA Library Quantification Kit. The Pooled library was then sequenced using the NextSeq 1000 Sequencing Kit.