library_construction_protocol: Nuclei were extracted in accordance with Ruiz Daniels et al. (2023) (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484441/). In brief, approximately 45 mg tissue samples were cut with scissors in 1 mL of TST buffer for 10 minutes on ice before being filtered through a 40 µm Falcon™ cell strainer (Thermo Fisher Scientific, catalog no. 08-771-2). A further 1 mL of TST and 3 mL of 1X PBS + BSA buffer were added to each sample before centrifuging at 4°C for 5 minutes at 500 g. Samples were resuspended in 1 mL 1X ST buffer filtered again through a 40 µm cell strainer, stained with Hoechst 33342 Solution (Thermo Fisher Scientific, catalog no. 62249) and then nuclei integrity was visually assessed using a fluorescent microscope. A disposable flow haemocytometer (C-Chip Neubauer Improved (100 µm depth), NanoEnTek, catalog no. DHC-N01) was then used to estimate nuclei counts. Samples were processed with Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) (10X Genomics) using the protocol outlined in the User Guide (CG000315 Rev C). Samples were diluted with nuclease-free water to a target concentration that would recover approximately 7000 nuclei in the final library. Samples were then loaded on the Chromium Controller for nuclei droplet formation. After subsequent nuclei and UMI barcoding and reverse transcription, resulting cDNA was then amplified, fragmented, and indexed with Truseq adapters and Illumina sample indexes.