design: Small segments from the middle section of the Physaloptera sp. sample were homogenized in 400 ul of SM buffer using a pestle. The homogenate was centrifuged at 10000 x g for 10 mins. 200ul of the supernatant was used for viral DNA extraction using High Pure Viral Nucleic Acid kit. 2 ul of the viral DNA extraction was used to enrich circular DNA molecules using rolling-circle amplification (RCA) with the phi29 kit (Watchmaker). This was then used to prepare Illumina sequencing library using Illumina DNA LP (M) Tag 96 sample preparation kit (Illumina, USA). This was sequenced at Psomagen Inc (USA) on an Illumina NovaseqX Psomagen Inc (USA).