library_construction_protocol: Tissues (ovary, uterus, thymus, brain, heart, intestine, kidney, liver and spleen) were freshly dissected and immediately transferred into RNA lysis buffer. Samples were mechanically homogenized using a tissue homogenizer. Total RNA was isolated using a kit-based extraction workflow according to the manufacturer's instructions. RNA concentration was measured (e.g., spectrophotometry/fluorometry), and RNA quality was assessed prior to submission for library construction and high-throughput sequencing. RNA libraries for RNA-seq were prepared using standard Illumina protocols.