design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 3.54 week-old seedlings, PEG-mediated CRISPRCas9 RNP transformation, amplicon library for gRNA targeting chr7;58179137;58179157;-_1st_rep (target sequence: GTCTTGGATACTACACGTGATGG) prepared using high-fidelity PCR