design: Cleavage reactions using  DNA were purified with the Monarch PCR & DNA Cleanup Kit (NEB). End-repair and dA-tailing were performed using the NEBNext Companion Module, followed by ligation of barcoded adapters (EXP-NB104, Oxford Nanopore). Libraries were cleaned with AMPure XP beads, quantified by Qubit, and pooled to 500ng input. Sequencing libraries were prepared using the SQK-LSK114 protocol and run on MinION R9.4.1 flow cells. Basecalling and demultiplexing were performed with Guppy v6.5.7 using default settings.