library_construction_protocol: Single-stranded circular DNA library was constructed using the MGIEasy RNA Library Preparation Reagent Group (MGI, China) with 100-200 ng of total RNA. In brief, the RNA was first fragmented and then reverse transcribed into cDNA. The remaining cDNA was converted into double-stranded DNA, followed by purification, end-repair, A-tailing, adapter ligation, and amplification. Finally, 60 fmol of the polymerase chain reaction (PCR) product was circularized and amplified by rolling cycle replication (RCR) to generate library based on DNA nanoballs (DNBs). Library was sequenced on the MGISEQ-200RS system (MGI, China) sequencing platform using MGISEQ-200RS High-throughput Sequencing Kit (MGI, China) with 100bp paired-end method. submitted_instrument_model: MGISEQ-200