design: The clean data without ribosome RNA reads for each sample were processed by fastx_toolkit (v0.0.0.14, http://hannonlab.cshl.edu/fastx_toolkit/) for quality control.  Then stringtie (v2.1.2, https://ccb.jhu.edu/software/stringtie/), cufflinks (v2.2.1, http://cole-trapnell-lab.github.io/cufflinks/) and kallisto (v0.46.2, https://pachterlab.github.io/kallisto/download) were used for transcript reconstruction, identification, quantification and expression analysis.