library_construction_protocol: Lungs were homogenised using pellet pestles and RNA extraction was performed using the Isolate II RNA mini kit according to manufacturer's instruction An input of 100 ng of total RNA was prepared and indexed for illumina sequencing using the TruSeq RNA sample Prep Kit with RiboGlobin depletion as per manufacturer's instruction. The indexed libraries were pooled and diluted to 1.5 pM for paired end sequencing (2 x 81 cycles) on a NextSeq 500 instrument using the v2 150 cycle High Output kit as per manufacturer's instructions. 1000 ng of the isolated total RNA was globin reduced and TruSeq Stranded kit was used for library preparation.