library_construction_protocol: RNA was extracted using the hot acid phenol method. Cell pellets were resuspended in 400 μl phenol solution saturated with 0.1 M citrate at pH 4.3 (Sigma; P4682) and 400μl of TES buffer was added (10 mM Tris pH 7.5, 5 mM EDTA, 1 % SDS). Samples were vortexed for 45 min at 65 °C and then centrifuged at 4 °C for 10 min. The supernatant was collected into a fresh tube and 400 μl phenol solution saturated with 0.1 M citrate at pH 4.3 was added. The samples were again vortexed for 20 min at 65 °C and then centrifuged at 4 °C for 10 min. The supernatant was collected into a fresh tube and 400 μl of chloroform was added (C2432; Sigma). The samples were vortexed briefly to remove phenol at room temperature and centrifuged at 4 °C for 10 min. The supernatant was collected into a fresh tube; 45 μl of 2 M LiCl was added and 1 ml of 95 % ethanol. Samples were precipitated for at least 30 min at – 80 °C, centrifuged for 25 min at 4 °C, washed with 400 μl of 80 % ethanol and after removing the supernatant dried at 37 °C. Pellets were resuspended in nuclease-free water and concentration was measured using Nanodrop apparatus. DRS libraries were prepared using Direct RNA Sequencing kit (ONT – Oxford Nanopore Technology, SQK-RNA002) according to the manufacturer's instructions, using 50–200 ng oligo-dT(25)-enriched mRNA from Saccharomyces cerevisiaeyeast mixed with cap-enriched or total RNA from other organisms (human, mouse, A. thaliana, or C. elegans), as described in Bilska et al. (2020). Sequencing was performed with MinION device (Oxford Nanopore Technologies, Flow cell type FLO-MIN106; RevD).