library_construction_protocol: RNA was extracted and purified using Quick DNA/RNA Magbead Kit (Zymo, USA) following the manufacturer's instructions and eluted in UltraPure distilled water (Thermofisher, U.S). Libraries were prepared using the Illumina stranded mRNA prep kit (Illumina, US) and sequencing was carried out with a NovaSeq 6000 SP 2x100bp FlowCell plus PhiX spike-in (Illumina, US).