design: Oral samples were collected by subjects swishing 15 ml of water for one minute. DNA was was extracted using DNeasy PowerSoil Pro Kit (Qiagen). 16S rRNA gene PCR primers 27F/1492R were used in a 35-cycle PCR, then purified using Ampure PB beads (Pacific Biosciences), and SMRTbell libraries prepared according to manufacturers guidelines. Circular Consensus Sequencing was completed following initial sequencing using PacBios CCS algorithm.