library_construction_protocol: RNA was extracted using TRIZOL protocol, RNA samples were purified using RNAclean XP beads (Beckman Coulter), and RNA concentration was measured with a NanoDrop Microvolume Spectrophotometer (Thermo Fisher). 1000-1500 µg of RNA was used as the starting material for cDNA library synthesis and tagmentation using the NEBNext Poly A kit (NEB) per the manufacturer's instructions.