library_construction_protocol: Total intracellular RNA was purified using the Monarch Nucleic Acid Purification Kit (New England Biolabs) RNA-seq libraries were prepared with the NEB-Next® Ultra™ II Directional RNA sample preparation kit (New England Biolabs, Inc., Ipswich, MA, USA).Messenger RNA was isolated from total RNA using poly-T oligo-attached magnetic beads. cDNA was synthesized, and libraries were prepared as previously described (Parkhomchuk et al., 2009).