library_construction_protocol: For the Chromatin immunoprecipitation (ChIP) assay, fresh hippocampal tissue were cut in small pieces and fixed using 1% formaldehyde for 10 minutes. The fixed tissue was then quenched and homogenized, followed by two washes in phosphate-buffered saline (PBS). Cells were lysed using 1 ml of lysis buffer from the EZ-ChIP kit (Millipore, Cat. No. 17-371RF), and chromatin was sheared to approximately 1000 bp fragments using a Diagenode pico bioruptor. The sheared chromatin underwent a preclearing step with Protein G beads. A 10 µl aliquot of this precleared chromatin was set aside as the input sample. The remaining chromatin was incubated with either anti-AVI antibody or preimmune IgG overnight at 4°C, followed by a 2-hour incubation with Protein Agarose G beads to capture the antibody-chromatin complexes. Post-incubation, the complexes were washed and eluted from the beads using 1% SDS and 0.1M NaHCO3 (pH 9.0) at 65°C. Crosslinks were then reversed by overnight incubation at the same temperature. DNA was deproteinized at 45°C for 2 hours and subsequently purified. Chromatin Immunoprecipitation sequencing (ChIP-Seq) was conducted at the University of Kansas Medical Center – Genomics Core using the Illumina NovaSeq 6000 Sequencing System. The procedure commenced with 5 ng of both fragmented input and immunoprecipitated chromatin, prepared using the TruSeq ChIP Sample Prep Kit (Illumina, Cat. No. IP-202-1012). The chromatin samples underwent end repair and 3' adenylation, followed by ligation with Illumina indexed adapters. Amplification of the library was performed using PCR with Illumina adapter-specific primers. The amplified libraries were then purified using KAPA Pure magnetic bead purification system (KAPA, Cat. No. KK8002).  Quality control and sizing of the prepared libraries were assessed using the Agilent TapeStation 4200 HS D1000 ScreenTape. Quantification was carried out on the Roche LightCycler96 using the FastStart Essential DNA Green Master qPCR kit (Roche, Cat. No. 06402712001), adjusting the ChIP-Seq libraries to a concentration of 1.9nM for pooling. Prior to sequencing, the libraries were denatured with NaOH, neutralized with 400mM Tris-HCl (pH 8.0), and diluted to 380 pM for optimal clustering. Clustering and sequencing were performed on a NovaSeq 6000 S1 flow cell using the S1 Reagent Kit v1.5 – 200 cycle (Illumina, Cat. No. 20028318). Post-sequencing, data were converted from .bcl to .fastq format using bcl2fastq software, followed by de-multiplexing to yield sample-specific sequences for downstream analysis.