library_construction_protocol: Isolated small intestinal lamina propria cell and PBMCs were washed and resuspended in 0.04% BSA in PBS. Cells were counted automatically with Countess II (Thermo Fisher, Waltham, MA) to determine their concentration. Single-cell RNA-sequencing libraries were the prepared using the Chromium Next GEM Single Cell 3' reagent kit v3.1 (10X Genomics, Pleasanton, CA) in accordance with the manufacturer's protocol. Briefly, the cells were diluted into the Chromium Next GEM Chip G as to yield a recovery of ~ 5,000 single-cell. Following the library preparation, the libraries were sequenced in multiplex on the Novaseq 6000 sequencer (Illumina, San Diego, CA) to produce on average a minimum of 60,000 reads per single cell.