library_construction_protocol: In brief, WT(mGfap-cre-RiboTag) and Stat3cKO (mGfap-cre-Ribotag-Stat3-loxP) mice were perfused with ice-cold PBS with heparin and spinal cords were dissected out. 3 mm of spinal cord rostral (T9- T11) and caudal (L1-L3) to the lesion epicenter were then rapidly removed, snap-frozen in dry ice and stored at -80C until processing for RiboTag RNA-Seq. Spinal cords were collected at 3,7, 14 and 28 dpi and anatomically equivalent regions of spinal cord were isolated from age- and genotype-matched healthy controls. Astrocyte RNA Integrity was analysed by a 2100 Bioanalyzer (Agilent) using the RNA Pico chip, RIN ≥ 8.9 for all samples. cDNA was generated from 10 ng of RNA using the Universal plus mRNA-Seq Kit (Nugen).