library_construction_protocol: The CMZ was dissected from the retinospheres or peripheral retinospheres were isolated and dissociated using Accutase and DNAse for 20-30 minutes on a nutator at 37C, with gentile pipetting to break up cells every 7 minutes. Cell pellets were resuspended in PBS containing 0.04% bovine serum albumin and filtered through a 35µm cell strainer (Fisher, 08-771-23) to remove cell clumps. Cells were then counted with a hemocytometer. 8,000-10,000 cells of the sample were then used as input into the 10X protocol. GEM generation, reverse transcription, cDNA amplification, and library construction steps were performed according to the standard 10x workflow for Single Cell 3 v3.1 Dual Index Gene Expression kit according to the manufacturers instructions.