design: Libraries were prepared from 1000 ng of RNA using the KAPA mRNA Capture Kit and KAPA RNA Hyper Kit (Roche), followed by final purification with KAPA HyperPure Beads (Roche). Libraries were pooled equimolarly and sequenced on a NextSeq 1000 (Illumina) using NextSeq 2000 P3 XLEAP-SBS Reagents (200 Cycles), generating 100 bp paired-end reads.