design: 500 ng of RNA was reverse transcribed using an oligo-dT primer with an Illumina-compatible sequence. The RNA template was degraded, and second-strand synthesis was performed with a random primer linked to an Illumina-compatible sequence. The resulting double-stranded cDNA was purified, amplified, and adapter sequences were added for cluster generation. The final library was purified and sequenced using single-end 75bp reads on the Illumina NextSeq 500 platform.