design: Total RNA was extracted from tissue samples using a standard extraction protocol. The mRNA was then enriched using poly-T oligo-attached magnetic beads that selectively bind the poly-A tails of eukaryotic mRNAs. Following purification, the mRNA was fragmented and reverse transcribed into cDNA. The cDNA library was prepared following the TruSeq Stranded mRNA Library Prep Kit (Illumina) protocol. The library was indexed and amplified using PCR. Sequencing was performed on an Illumina NovaSeq 6000 platform with a 150 bp paired-end read configuration, achieving a minimum of 40 million reads per sample.