library_construction_protocol: Utilizing the miRNeasy serum/plasma kit, total RNA was isolated from the plasma.(Qiagen, catalog number 217184). Plasma (200 µL) was mixed with 5 volumes of QIAzol, vortexed and then incubated for 5 minutes at room temperature. After vortexing and incubating, the mixture was centrifuged at 12,000 rpm. After being poured into a fresh test tube, the supernatant was vortexed and combined with 1.5 times the volume of anhydrous ethanol. The sample was loaded on the chromatographic column and centrifuged at 13,000× g. After discarding the flow-through liquid, the chromatographic column was filled with the buffer RPE (500 µL) and centrifuged. Add anhydrous ethanol (80%) to the column and centrifuge. Once the flow-through liquid has been disposed of, centrifuge the column and empty it. After adding 14 µL of RNase-free water to the column's core, the RNA was centrifuged to extract it, and it was then kept cold—at -70°C. By the guidelines provided by the manufacturer, the All-in-One miRNA qRT-PCR Detection Kit(QP015, GeneCopoeia, Rockville, MD, USA) was used to perform real-time PCR to quantify mature miRNAs. While the forward primers (Supplementary Table 1) were designed using the mature miRNA sequence, the reverse primers were provided by the kit manufacturer. Reverse transcription (RT) and PCR are the two phases in quantitative analysis. In a total amount of 10 μl, there was 0.5 μg of RNA, 5 μl of 2×TS miRNA Reaction Mix, and 0.5 μl of TransScript miRNA RT Enzyme Mix for every RT reaction. The gene amp PCR system 9700 (Applied Biosystems, USA) was used to perform the reaction for 60 minutes at 37°C. After that, it was thermally inactivated for 5 seconds at 85°C. The RT product was kept at -20°C after being diluted ten times in water devoid of nuclease.