library_construction_protocol: Non-adherent iCD103-DCs were collected on days 15-16. Total RNA were isolated with RNA Extraction Kits. Total RNA was used as the starting material for RNA sample preparation. mRNA was purified from the total RNA using poly-T oligo-attached magnetic beads. Fragmentation was performed with divalent cations at an elevated temperature in First Strand Synthesis Reaction Buffer (5X). First-strand cDNA was synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H-). Second-strand cDNA synthesis followed, utilizing DNA Polymerase I and RNase H. Remaining overhangs were converted into blunt ends via exonuclease/polymerase activity. After adenylation of the 3' ends of DNA fragments, adaptors with a hairpin loop structure were ligated for hybridization. cDNA fragments, preferably 370–420 bp in length, were purified using the AMPure XP system. PCR was then performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers, and Index (X) Primer. Finally, PCR products were purified using the AMPure XP system, and library quality was assessed using the Agilent Bioanalyzer 2100 system.