library_construction_protocol: at 16 weeks of diet, gonadal/visceral  white adipose tissue (GWAT or VAT) was colected and snap freeze in liquid nitrogen, and kept at -80 until RNA extraction. 100 mg of fat tissue was in 1 ml of QIAyol Lysis buffer (RLT Plus + DX). To reach the miligrams of weight GWAT from 3 mice need it to be pooled from HoipA-KO, WT controls were treated in the same way for consistency .  5 mm bead was use to homogenised the tissue in TissueLyser II in two rounds for 2 minutes at 30 1/s. Samples were processed according to the manufacturer's instructions using the kit RNAeasy Lipid Mini Kit (74804, Qiagen). A DNase digest kit was employed, involving the addition of 350 µl of Buffer RW1 to the RNeasy Mini spin column, followed by the addition of DNase I. The resulting RNA was evaluated for purity and concentration using a Nanodrop spectrophotometer from TECAN Reader (model) For RNA sequencing, we used 2 μg of total RNA per sample concentration range 50-200 ng/µL, at least 10µL volume, checking the quality by RIN > 7, OD260/280 = 1,8-2,1 and OD260/230 >1,5. mRNA libraries were prepared by mRNA Seq (Poly A+ selection) using ERCC RNA Spike-In controls. Libraries were prepared using the Illumina® Stranded TruSeq® RNA sample preparation kit. ERCC RNA Spike-In Mix (Thermo Fischer) was added to the samples before library preparation. Library preparation started with 1000 ng total RNA. After poly-A selection (using poly-T oligo-attached magnetic beads), mRNA was purified and fragmented using divalent cations under elevated temperature. The RNA fragments underwent reverse transcription using random primers. This was followed by second strand cDNA synthesis with DNA Polymerase I and RNase H. After end repair and A-tailing, indexing adapters were ligated. The products were then purified and amplified (15 cycles) to create the final cDNA libraries. After library validation and quantification (Agilent Tape Station), equimolar amounts of library were pooled. The pool was quantified by using the Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System. The pool was sequenced on an Illumina NovaSeq6000 sequencing instrument with a PE100 protocol aiming for 40 million clusters per sample.