design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 1420 day-old seedlings (from BetterSeeds company), PEG-mediated CRISPRCas9 RNP transformation, UMI-DSBseq amplicon library targeting chr3;741000;741020;-_1st_rep (target sequence: ATATTGTATGAATGATTTACGGG) prepared for paired-end Illumina sequencing (either with Novaseq 6000 or Nextseq 500)