library_construction_protocol: Eighteen samples were prepared for single cell transcriptomic analysis using the 10X 3' Gene expression kit.  Samples were diluted to 10,000 cells and partitioned using the Chromium iX Controller, the Next GEM chip G and the single cell 3' kit v3.1 library preparation kit (10x Genomics, Pleasanton, California, United States). Sequencing library quality was determined using the Qubit DNA BR kit (Life Technologies, UK), Illumina library quantitation kit (NEB, US) and the Tapestation D5000 DNA BR kit (Agilent, US).  Sequencing libraries were pooled randomly across 2 NextSeq 2000 P4 100 cycle kit (Illumina, San Diego, California, United States), each with 1% PhiX spikes, aiming for approximately 200 million reads per sample.  Runs formats aligned with manufacturers recommendations