library_construction_protocol: Cell monolayers were then transferred into ice-cold PBS and subject to ultraviolet (UV) cross-linking (254 nm) at 150 mJ/cm2 using a Stratalinker Ultraviolet Crosslinker (Stratagene). After cell lysis and limiting RNase I digestion, 4HA-LegC4 was immunoprecipitated from four independent infection experiments using Anti-HA-tag mAb-Magnetic Beads (MBL Biosciences Cat#M180-9). cDNA libraries were prepared according to the iCLIP2 protocol (Buchbender et al, 2020).