design: The samples were resuspended in 100ul water. The initial concentration of DNA was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). To determine the size of the DNA, samples were run into E-Gel SizeSelect 2% Agarose Gel (Invitrogen) along with 1Kb ladder. The sample showed DNA band of >12000 bp.  The samples were sheared using the Covaris G-tube (Covaris Inc.). The average size of the sheared DNA was determined (Table 1) using Agilent 2100 Bioanalyzer (Agilent Technologies). 2500 ng of the sheared and purified DNA was used as input for the library preparation using SMRTbell Express Template Prep Kit 2.0 (Pacific Biosciences). During library preparation, the samples underwent DNA damage and end repair as well as barcode adapter ligation. To remove the unligated DNA fragments from the libraries, nuclease treatment was performed. The SMRT libraries were size selected (>6Kb) using 0.75% Agarose gel in Blue Pippin (Sage Science). Following the size selection, the concentration of the final libraries was measured using the Qubit dsDNA HS Assay Kit (ThermoFisher Scientific). The libraries were then sequenced using the 30-hour movie time on the PacBio Sequel II (Pacific Biosciences).