library_construction_protocol: MCF7 cells were lysed and homogenized using Trizol reagent (ThermoFisher Scientific) immediately upon harvesting. RNA extraction was subsequently performed using the Ambion PureLink RNA Mini Kit (ThermoFisher Scientific), following the manufacturer's protocol, with the inclusion of an on-column DNase I digestion step to eliminate genomic DNA contamination. RNA quality and purity were verified with a Nanodrop 2000 spectrophotometer, accepting RNA samples with A260/A280 and A260/A230 absorbance ratios around 2.0 as sufficiently pure for downstream applications​. RNA integrity numbers were determined using an Agilent Bioanalyzer 2100 and typically were above 9 and were used for cDNA production. One microgram of total RNA was used for isolating polyA-purified mRNA and subsequently used for cDNA-library construction with the Illumina TruSeq RNA Preparation Kit.  Strand specificity was performed with 2'-deoxyuridine 5'-triphosphate during second-strand synthesis. All samples were indexed with Illumina adapters.