design: DNA extracted from 1 ml of human fecal culture pellet using DNeasy UltraClean Microbial Kit (Qiagen). DNA concentrations measured using Qubit 2.0 dsDNA HS kit (Qiagen) and adjusted to 5 ng/ before PCR. V3-region 16S rRNA genes was sequenced using the Ion Torrent GSS5 platform in DTU DMAC core as previously described (Christensen et al. 2014).