design: The 6 leaves from each group were harvested and immediately frozen in liquid nitrogen for RNA-extraction at 48 hpi and 96 hpi, respectively with three biological replicates in each treatment at state key laboratory in Yunnan Agricultural University. Total RNA was extracted from rice leaves using plant RNA kit (OMEGA, USA) and observed RNA degradation and contamination on 1% agarose gels, and then utilized for purity using a NanoPhotometer spectrophotometer (IMPLEN, CA, USA). RNA concentration was measured using the Qubit RNA Assay Kit in a Qubit 2.0 Fluorimeter (Life Technologies, CA, USA) and the integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA).