library_construction_protocol: Spleens were harvested from PLP178-191-immunized mice at day 10 and homogenized using 200 U/ml of collagenase (Worthington Type 3) and 10 mM EDTA (Invitrogen). The homogenates were incubated at 37℃  for 30 minutes and made into single-cell suspensions. Antigen-presenting cells were enriched using Nycodenz gradient centrifugation. Briefly, cells were washed with RPMI and resuspended in 5 ml of Nycodenz working solution and 5 ml of 1% HBSS was overlaid spun at 1800g for 15 minutes without deceleration. The isolate was collected, and dendritic cells (DC) were further enriched using magnetic bead sorting (CD11c MicroBeads UltraPure, Miltenyi Biotech, Auburn, CA) with > 90% purity. Splenic DC were isolated from CD8 recipients, PLP-CD8-DC and OVA-CD8-DC, on day 12 post-EAE induction. DC were strained through a 40-µm filter, fixed using the Cell Fixation Kit and processed with the Evercode Whole Transcriptome Mini Kit from Parse Biosciences (Seattle, Washington, USA) according to the manufacturer's guidelines. Sub-libraries were evaluated for adequacy (Agilent BioAnalyzer RNA integrity number >8) and sent for Next Generation Sequencing on the Illumina NovaSeq 6000 using 100bp paired-end reads (Iowa Institute of Human Genetics, University of Iowa).