library_construction_protocol: Total RNA was extracted using the RNeasy® Mini Kit (Qiagen, 74904). RNA quality was assessed using a NanoDrop One spectrophotometer Thermo Fisher Scientific, Waltham, MS, USA), and only samples with an RNA Integrity Number of 9.0 or higher were used for further analysis. Libraries were prepared using the QIAseq UPX 3' Transcriptome Kit (Qiagen, Venlo, Netherlands, 333088) and the MGIEasy Universal Library Conversion Kit (App-A) (MGI, Shenzhen, China, 1000004155). Paired-end sequencing (150 bp) was performed on the DNBSEQ-G400 platform (MGI, Shenzhen, China). The quality of raw sequencing reads was evaluated using FastQC, and adapters and low-quality reads were removed using Trim Galore!, Trimmomatic, and Cutadapt. Clean reads were mapped to the Rat (Rattus norvegicus) genome assembly mRatBN7.2 using the STAR aligner, and gene-level read counts were obtained using featureCounts. Differential gene expression analysis was conducted using the DESeq2 package (version 1.20.0). Genes with a false discovery rate (FDR) < 0.05 were considered statistically significant.