library_construction_protocol: Frozen liver tissue was isolated for single nucleus RNA sequencing using the Chromium Nuclei, Isolation Kit (PN-1000494,10x Genomics) according to manufacturer's protocol. 10,000 cells were used to prepare snRNA-seq libraries. Chromium Single cell 3' Library and Gel Bead Kit V3.1 (10×Genomics, PN1000268) was used to generate single cell gel beads in emulsion (GEM). The captured cells were lysed, and the released RNA was reverse-transcribed with primers containing poly-T, barcode, unique molecular identifiers (UMIs) and read 1 primer sequence in GEMs. Barcoded cDNA was purified and amplified by PCR. The adapter ligation reaction was performed to add sample index and read 2 primer sequence. After quality control, the libraries were sequenced on Illumina Novaseq 6000 platform in 150 bp pair-ended manner (Berry Genomics Corporation, Beijing, China).