library_construction_protocol: Mouse spinal cord spatial transcriptomics was performed by Visium (10x Genomics). iSCI mice were perfused with ice-cold PBS with heparin at 3, 7, or 28 dpi, spinal cords dissected out and rostral and caudal blocks were rapidly embedded in OTC, snap frozen on dry ice and stored at - 80°C until sectioning. Visium slides were pre-chilled in a cryostat (Leica) for 30 minutes at the time of sectioning. Two 10um sections were taken from lesion-remote rostral (T9-T11) and caudal (L1-L3) blocks, equivalent to samples analyzed by snRNA-Seq. 4 tissue sections were placed onto each square, with uninjured on the top left, 3dpi on the top right, 7dpi on the bottom left, and 28dpi on the bottom right. Samples were processed using the Visium Spatial Gene Expression Reagent Kit (10X Genomics) as per manufacturer's established protocol. cDNA libraries were pooled in a NovaSeq6000 SP v1.0 flowcell and paired-end sequencing was performed on an Illumina NovaSeq6000 sequencer.