design: The Nanopore libraries were prepared from the QC passed genomic DNA sample using Ligation Sequencing-gDNA Native Barcoding Kit. Briefly, 800ng of DNA sample was processed for DNA repair followed by AMPureXP purification. The purified DNA sample was processed for adaptor ligation followed by pooling as per the kit ecommendation. The pooled libraries were purified using AMPureXP bead followed by Qubit quantification. The purified library pool was loaded onto Oxford Nanopore PromethION P2 Solo and processed for sequencing