library_construction_protocol: RNA was extracted using MaXtract High Density (QIAGEN, Redwood City, CA) according to the manufacturer's instructions. Integrity of the RNA was analyzed by a ND-1000 V3.7.1, according to the manufacturer's instructions. Only samples with an RNA integrity number (RIN) of 9 or greater were sequenced. RNA samples contained 1000-2000 ng in 50 ul of RNAse/DNAse free water were used for the library construction according to manufacture recommendations; RNAseq libraries were eluted in 30 μl of elution buffer. Then they were adjusted to 10 nM pool, the adjusted pool was loaded into Nextseq 500 machine (Illumina), as 76 bp single end read using the NextSeq 500/550 High-Output v2.5 Kit (75 cycles).  Binary Base Call (BCL) output files from a NoveSeq machine were converted to FASTQ format, using BCL to FASTQ (bcl2fastq v2.20.0.422 Copyright (c) 2007–2017 Illumina, Inc.)