library_construction_protocol: A total of 50,000 cells were subject to the CUT&Tag assay performed by SeqHealth (Wuhan, China) using a commercial kit (Vazyme, China, TD903) according to the manufacturer's protocol. Briefly, cells were incubated with Concanavalin A-coated magnetic beads. Subsequently, anti-NFATC2 or IgG control antibody were added, and the cells were incubated on a nutator overnight at 4℃. After that, secondary antibody was performed on a nutator for 1h at room temperature followed by incubation with hyperactive pG-Tn5 transposonase. After transposon activation and tagmentation, DNA was extracted, amplified, and purified to construct library. To amplify libraries, 21μL DNA was mixed with 2μL of a universal i5 and auniquely barcoded i7 primer. A volume of 25 μL NEBNext HiFi 2× PCR Master mix was added and mixed. The sample was placed in a Thermocycler with a heated lid using the following cycling conditions: 72 °C for 5 min (gap filling); 98 °C for 30 s; 14 cycles of 98 °C for 10 s and 63 °C for 30 s; final extension at 72 °C for 1 min and hold at 8 °C. libraried clean-up was performed XP beads (Beckman Counter).