design: Total RNA from the liver was extracted using TRIzol (Thermo Fisher), following the manufacturers instructions. Samples were then treated with RNase-Free DNase Set (QIAGEN) and again purified with RNAClean XP Kit (Beckman Coulter). Library was prepared with VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme). Next Generation Sequencing was performed with a NovaSeq6000 (Illumina) to generate 12G 2  150 bp paired-end reads per sample.