design: Nuclei were isolated from mouse hypothalamus using the GEXSCOPE Nucleus Separation Solution (Singleron Biotechnologies), filtered, resuspended in PBS containing 1% BSA, and stained with DAPI. DAPI-positive singlet nuclei were loaded onto a microfluidic chip, and snRNA-seq libraries were prepared using the GEXSCOPE Single Nucleus RNA-seq Kit (Singleron Biotechnologies) according to the manufacturers instructions. Libraries were sequenced on an Illumina NovaSeq X Plus with paired-end 150 bp reads.