library_construction_protocol: The extraction of RNA was carried out with the FastPure Plant Total RNA Isolation Kit (Vazyme, RC401), strictly following the protocol provided by the manufacturer. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.