design: The full-length 16S rRNA gene was amplified by using the barcoded primers provided by 16S barcoding kit 1-24 (SQK-16S024, Oxford-Nanopore Technologies). Amplification was performed with the following PCR conditions: initial denaturation at 95C for 1 min, 25cycles of 95C for 20 s, 55C for 30 s, and 65C for 2 min, followed by a final extension at 65C for 2min. The amplified products were purified using AMPure XP beads and quantified using Qubit dsDNA HS and BR Assay Kits (Thermo Scientific, Catalog number: Q32854). A total of 100ng of DNA was incubated with 1l of Rapid Adapter at room temperature for 5min. The prepared DNA library (11l) was mixed with 34l of Sequencing Buffer, 25.5l of Loading Beads, and 4.5l of water, loaded onto the (FLO-MIN106; Oxford Nanopore Technologies), and sequenced on the MinION. MINKNOW software ver. 1.11.5 (Oxford Nanopore Technologies) was used for data acquisition. Guppy basecaller (Oxford Nanopore Technologies) was used for base calling the MinION sequencing data (FAST5 files) to generate pass reads (FASTQ format) with a mean quality score>14