library_construction_protocol: Fibroblasts were washed in PBS and homozenized in Trizol reagent. RNA was precipitated from the aqueous phase with isopropanol and centrifugation at 4C, washed with 75% ethanol, and resuspended in DEPC-treated water. Genomic DNA was removed with an RNeasy Plus Mini Kit (QIAGEN) PolyA RNA was enriched and stranded libraries were prepared for sequencing using Illumina's TruSeq Stranded mRNA sample preparation kit.