design: Sample 16 DNA was extracted using the ZymoBiomics DNA Miniprep Kit and used for library preparation at the University of Toronto. The V4 hypervariable region of the 16S rRNA gene was amplified using a universal forward sequencing primer and a uniquely barcoded reverse sequencing primer.  Pooled duplicates were quantified using PicoGreen and combined. The library was then purified using Ampure XP beads and loaded on to the Illumina MiSeq. Sequencing was performed using the V2 150bp paired end reads chemistry.  A single-species, Pseudomonas aeruginosa DNA, a mock community, Zymo Microbial Standard, and a template-free negative control were included in the sequencing run