design: Barcoded sequencing libraries were prepared with ONT Native Barcoding Kit 24 V14 (SQK-NBD114.24) using 200fmol input for Batches 1-3 and varying concentrations for bacterial cultures (200, 125, 50, 0fmol) and sequenced on a MinION Mk1B device. Post-sequencing analysis was performed in MinKNOW Version 24.11.10.