library_construction_protocol: Cells were collected by centrifugation, resuspended in TRIzol LS reagent followed by incubation at room temperature for five minutes. The homogenized samples were incubated with chloroform followed by centrifugation. The aqueous layer was transferred to an RNAeasy spin column and the Qiagen kit protocol for RNA extraction was followed (Qiagen # 74134). RNA QC analysis, mRNA library preparation (poly A enrichment) and sequencing (PE150, 15G raw data per sample) using NovaSeq X plus Series was carried out by Novogene Corporation. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. (cited from Novogene sequencing report)