design: RNA were extracted by FastPure Cell/Tissue Total RNA Isolation Kit V2 (VAHTS). The integrity of RNA was determined with the Agilent 2100 Bioanalyzer (Agilent Technologies, Inc., USA). The purity and concentration of the RNA were determined with the Nanodrop (Thermo Fisher Scientific, USA) and Qubit 4.0 Fluorometer (Invitrogen, USA). RNA libraries were constructed following the manufacturers instructions (MGIEasy RNA Library Prep Set) and sequencing of RNA-seq were performed on the MGISEQ-2000 platform.