design: The samples were derived from soil. The genomic DNA of the samples was extracted and then the purity and concentration of the DNA were detected by an agarose gel electrophoresis. 16S rRNA genes of distinct regions (16SV4) were amplified used specific primer (515F- 806R) with the barcode. All PCR reactions were carried out with Phusion High - Fidelity PCR Master Mix (New England Biolabs). The constructed library was quantified by Qubit and Q-PCR. After the library was qualified, it was sequenced by illumina NovaSeq 6000.