library_construction_protocol: Total RNAs were extracted from the pancreatic islets of virgin and GD14.5 female mouse by using the TRIzol Reagent (Cat# 15596026, Invitrogen). The extracted RNA samples were enriched with eukaryotic mRNA using magnetic beads with Oligo (dT), followed by terminal repair, poly (A) addition, sequencing junction, and PCR amplification.