design: Metagenomic libraries were constructed utilizing the iNextEra library preparation protocol. The DNA was tagmented using Illumina bead-linked transposomes (Illumina DNA library prep, Illumina, Inc., San Diego, CA, USA) and 2X TMP buffer (Ref) in a total volume of 6 L, comprising 3 L of 2X TMP buffer, 0.5 L of bead-linked transposome, and 2.5 L of DNA, followed by incubation at 53C for 30 minutes. After tagmentation, a limited-cycle PCR step finalized the Illumina-compatible adapters with dual-indexes utilizing custom iNextEra primers for sequencing of pooled libraries