library_construction_protocol: The nuclei isolated by different methods were put into sterile PCR tubes containing reagents as per the 10x user guide. GEMs were generated using the 10x Genomics Chromium Controller with Next GEM Chip J single cell kit (10x Genomics Inc., Pleasanton, CA, USA). After pre-amplification PCR, all libraries are divided into two parts, one part is used to perform snRNA-seq library, while the other is used to perform snATAC-seq library according to CG000338_ChromiumNextGEM_Multiome_ATAC_GEX_User_Guide_RevA.