library_construction_protocol: Tissue Quality Assessment and Nucleic Acid Extraction: Tissue quality was initially assessed by haematoxylin–eosin staining of 6 µm cryostat sections. Up to 40 mg of frozen tissue was lysed in Qiagen RLT Plus buffer and homogenized by repeated passage through an insulin syringe. DNA and RNA were co-extracted using the AllPrep DNA/RNA Mini Kit (Qiagen, Hilden, Germany), following the manufacturer's protocol. RNA-seq Library Preparation and Sequencing: RNA-seq libraries were prepared using the QuantSeq 3′ mRNA‑Seq Library Prep FWD kit (Lexogen, Vienna, Austria). This process generated approximately 5 million 75 bp reads per sample. Sequencing was performed on an Illumina NextSeq 550 (Illumina, San Diego, CA) at the SELA facility (USP).