design: otal RNA was extracted from RNA shield-preserved insects using Qiagens RNeasy Fibrous Tissue Mini Kit following the manufacturer's protocol. RNA purity and concentration were assessed using a Nanodrop (Thermo Fisher Scientific, Waltham, MA), Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA), and an Agilent 4200 TapeStation System (Agilent, Santa Clara, CA). All samples had an RIN number above 8 to limit prepping samples with polyA tail degradation. Libraries were constructed with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760S) with the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490S) for mRNA enrichment and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 1). Libraries were pooled and sequenced on an SP cell on a Illumina NovaSeq6000.