library_construction_protocol: Total RNA was extracted using the Qiagen RNeasy Mini Kit following the manufacturer's protocol. RNA quality and concentration were assessed using Qubit and Agilent Bioanalyzer. RNA libraries were prepared using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina®. Poly(A) RNA was enriched using oligo(dT) beads, followed by fragmentation, cDNA synthesis, end repair, adapter ligation, and PCR enrichment. Library quality was checked with Qubit and Bioanalyzer.