library_construction_protocol: Tumors samples were ground in a homogenizer and lysed using the TRIzol method (Takara) to release total RNA. The RNA was then extracted with chloroform, precipitated with isopropanol, washed with 75% ethanol, and finally dissolved in RNase-free water for quantification and quality control. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dTTP for non-directional library. For the non-directional library, it was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries will be pooled and sequenced on Illumina platforms, according to effective library concentration and data amount.