design: gDNA samples are converted to Ultima sequencing libraries using the NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina Sample Preparation Kit (Cat. # E7430L). The fragmented DNA is ligated to IDT x-Gen PCR Free Adapters for Ultima (Cat. #10016841). The libraries are size-selected using Beckman Coulter SpriSelect beads( Cat. # B23318).