design: 1g of prepared total RNA with the concentration  50 ng/L, RQN>7, OD260/280 between 1.8 and 2.2 was ligated to the 5 and 3 adaptors and reversely transcribed into cDNAs using the QIAseq miRNA Library Kit (Qiagen, German). The adapter-ligated cDNA was enriched by PCR, then purified and size-selected on a 6% Novex TBE PAGE gel to isolate small RNA fragments of 1830 nt. Final libraries were sequenced using 140160 bp single-end reads on the NovaSeq X Plus platform at Majorbio Bio-Pharm Technology Co., Ltd., Shanghai, China.