library_construction_protocol: Cryopreserved samples were used to generate a single cell suspension (for scRNA-seq) or a single nuclei suspension (for single-cell Multiome, according to protocol Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing [10x Genomics]). Single cells (for scRNA-seq) or nuclei (for scMultiome ATAC+Gene Expression) were captured on a 10x Chromium Controller instrument according to manufacturer instructions. Libraries were generated using Chromium NextGEM Single-cell 3' Gene Expression reagents (for scRNA-seq) or using the Chromium Next GEM Single-cell Multiome ATAC + Gene Expression reagents (for scMultiome). Quality control on intermediate products and final libraries was performed using the Agilent Bioanalyzer High Sensitivity Kit. The final libraries were quantified via qPCR using KAPA Library Quantification Kits. A spike-in of 1% PhiX control library (Illumina) was used as the technical control.