library_construction_protocol: Freshly harvested mouse tumor tissues (KPC-shV, n=2; KPC-shZEB1, n=2) were sectioned into appropriately sized fragments, embedded in OCT, and rapidly frozen in liquid nitrogen. Tissue blocks were then subjected to methanol fixation, followed by Hematoxylin & Eosin (H & E) staining, imaging, and destaining, according to the experimental protocol recommended by 10x Genomics (Pleasanton, CA, USA). Subsequently, tissue sections were mounted onto a 10x Visium CytAssist Spatial Gene Expression Slide. This slide contains two capture areas, each measuring 6.5 x 6.5 mm, with approximately 5,000 barcoded spots per area, designed for mRNA capture via oligonucleotides. Following the guidelines from 10x Genomics, probe hybridization and release were performed, and then the probes were transferred to the Visium CytAssist slide. Library construction was subsequently carried out using the Visium CytAssist Spatial Gene Expression for FFPE kit (PN-1000521). The DNA libraries were sequenced using the PE-150 mode.