design: DNA was extracted using the long-fragment DNA magnetic bead method. After being disrupted by Megaruptor (approximately 15-20K), the DNA underwent damage repair and end repair before being ligated with known adaptors. Following enzymatic digestion, selection, and final transformation into a dumbbell-shaped HiFi library, it was subjected to quality checks. Post-qualification, the sample underwent PacBio sequencing, subsequent data analysis, and interpretation of results.