design: Total RNA was extracted from leaf tissue and its quality was verified using a NanoDrop 2000 spectrophotometer and an Agilent 2100 Bioanalyzer. mRNA was enriched from total RNA using oligo(dT) magnetic beads and then fragmented. First- and second-strand cDNA were synthesized from the fragmented mRNA. The double-stranded cDNA was purified, end-repaired, 'A'-tailed, and ligated to sequencing adapters. Size selection was performed using AMPure XP beads, followed by PCR amplification to enrich the final cDNA library. The constructed libraries were quantified using Qubit 3.0 and Qsep400, and their quality was confirmed by Q-PCR. Sequencing was performed on an Illumina NovaSeq 6000 platform in PE157 mode.