library_construction_protocol: Cells were incubated with dissociated with accutase at 37 C for 30 minutes. They were resuspended inice-cold PBS. Multiseq conjugated oligos were combined according to manufacturer's protocols which briefly included adding the anchor oligo on ice,  followed by the co-anchor, then the barcode. Cells were pelleted, resuspended in PBS + 1% BSA. Cells were sorted using live-dead DAPI staining with FACS. Cells were pooled and prepared for future processing. Libraries were constructed following the manufacturer's protocol