library_construction_protocol: Non-leukemic recipient cells were enriched through CD45.1 positive selection followed by lineage depletion. Lin- cells were further purified through cell sorting prior to library preparation Cells were loaded onto a 10X Chromium Controller and prepared for scRNA-seq according to the manufacturer protocol, including library generation (10X Genomics, v3.1). Next Generation Sequencing was performed by the Genomics Core at Nationwide Children's Hospital using 2x150 bp reads on a NovaSeq SP instrument.