design: Fetal-like reversion in the regenerating intestine is regulated by mesenchymal Asporin
library_construction_protocol: Freshly isolated small intestinal crypts were cultured as organoids in 60% matrigel for 48 hours, with or without recombinant asporin. Organoids were collected from matrigel and total RNA was extracted from them. Total RNA was isolated using RNeasy Mini Kit (Qiagen) according to the Manufacturer's instructions with on-column DNAse digestion. An Ovation Universal RNA-seq System kit was used for Illumina library preparations (NuGEN Technologies Inc., CA, USA), with rRNA removal according to the kit manual. Libraries were purified with AMPure XP beads.