design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 3.54 week-old seedlings, PEG-mediated CRISPRCas9 RNP transformation, amplicon library for gRNA targeting chr4;363875;363895;+_1st_rep (target sequence: ACATTTCTGTTTACAGGTTGTGG) prepared using high-fidelity PCR