library_construction_protocol: Total RNA was extracted from cardiomyocytes derived from all three FRDA and isogenic control iPSC lines across three independent experiments using the Cytivia Mini RNA Extraction Kit (Cytivia, MA, USA), following the manufacturer's instructions. Residual genomic DNA was removed by treatment with TURBO DNase (Thermo Fisher Scientific). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies), and all samples exhibited an RNA Integrity Number (RIN) of approx 10. Library preparation was performed using 100 ng of input RNA per sample with the TruSeq Stranded Total RNA Library Prep Kit (Illumina, CA, USA) as per manufacturer's instruction.  The library was quantified using the Agilent Tapestation and the Qubit™ RNA assay kit for Qubit 2.0® Fluorometer (Life technologies).  Unique dual indexes (Illumina TruSeq RNA UD Indexes) were used to barcode individual libraries.  The indexed libraries were then prepared and diluted to 750pM for paired end (2x 101 base) sequencing on a NextSeq2000 instrument using the P3 200 cycle kit using v3 chemistry (Illumina, CA, USA) as per manufacturer's instructions.