library_construction_protocol: the genomic DNA was extracted from transfected cells, infected embryos, and cattle ear-tips using the TIANamp Genomic DNA Kit (Tiangen) or QIAamp DNA Mini Kit (Qiagen) according to the manufacturer's instructions. Genomic sites of interest were amplified using unique barcoding primer pairs with Phanta Max Super-Fidelity DNA Polymerase (Vazyme) for deep sequencing. The PCR products were pooled by common amplicons and sequenced with pair-end 150 bp on the HiSeq 4000 system (Illumina).