library_construction_protocol: Total RNA was extracted from endometrial organoids using TRIzol® Reagent (Thermo Fisher Scientific, Waltham, MA, USA) and purified with the RNA Purification Kit (MajorBio Technology Co., Ltd, Shanghai, China). Stranded mRNA libraries were prepared using the Illumina® Stranded mRNA Prep, Ligation Kit (Illumina, San Diego, CA, USA). The process included poly(A) selection to isolate mRNA, fragmentation to achieve an average insert size of 300 bp, and ligation of sequencing adapters.