design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 3.54 week-old seedlings, PEG-mediated CRISPRCas9 RNP transformation, amplicon library for gRNA targeting chr7;49517066;49517086;-_1st_rep (target sequence: ATAGTGTCCCTTTTAACCAAAGG) prepared using high-fidelity PCR