library_construction_protocol: After confirmation of the PDPN model by behavioral and electrophysiological assessments, the rats were euthanized and the dorsal root ganglia (DRGs) from the L4–L6 spinal segments were rapidly dissected, snap-frozen in liquid nitrogen, and stored at –80 °C.Total RNA was extracted using the TRIzol reagent  (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with qualified purity, quantity and integrity were used for subsequent library construction. Ribo-off rRNA Depletion Kit（vazyme, Nanjing, China）was used to remove ribosomal RNA The libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.