library_construction_protocol: Mice were harvested at Weeks 6 post-final injection, and CD45⁺ immune cells were sorted from kidneys using flow cytometry and subjected to scRNA-seq using the 10× Genomics platform. Single-cell 3' biased transcriptome and featuring barcoding libraries were prepared using a 3' Library & Gel Bead Kit (PN-1000268) and 3' Feature Barcode Kit ( PN-1000262) according to the 10x Genomics manual. Libraries were sequenced by illumina Novaseq X plus machine