design: An aliquot of the same genomic DNA used for long-read sequencing was mechanically sheared using a Covaris ultrasonicator to generate random fragments with a target size distribution centered around 350 bp. The fragmented DNA underwent a sequential enzymatic treatment process, beginning with end-repair to create blunt ends, followed by the addition of a single adenine nucleotide to the 3' ends (A-tailing). Subsequently, Y-shaped Illumina adapters with a corresponding 3' thymine overhang were ligated to both ends of the A-tailed DNA fragments. The adapter-ligated product was then size-selected using AMPure XP beads to remove excessively short or long fragments and isolate the desired insert size range. Finally, the size-selected library was subjected to a limited number of PCR cycles to amplify the library material, ensuring sufficient quantity for sequencing on the Illumina NovaSeq platform.