design: The whole genome amplification was performed using the QIAseq Fx Single cell kit (Qiagen, Hilden), and DNA libraries were prepared using the Illumina DNA Prep Kit (Illumina, San Diego, CA, USA). Paired-end nucleotide sequencing (151bp x 2) was performed on the Illumina MiSeq platform (Illumina, San Diego, CA, USA) using a MiSeq Reagent Kit v3 at the University of Free State Next Generation Sequencing (UFS-NGS) Unit