library_construction_protocol: RNeasy Micro Kit (Qiagen, catalog no. 74004) was utilized for RNA extraction. NanoDrop 2000 Spectrophotometer (Thermo Scientific, catalog no. ND-2000) was utilized to determine purity and concentration. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using randomhexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library ordTTP for non-directional library.