library_construction_protocol: RNA from Caco2 WT and PIWIL2 KO cells was isolated by adding 1ml of Trizol (Invitrogen # 15596018), incubating the sample at RT for 5 minutes then using the PureLink RNA mini kit (Invitrogen #12183018A) for total RNA, using the whole transcriptome protocol of the kit. Final RNA concentrations and purity were determined by using a NanoDrop spectrophotometer. Short RNA sequencing was performed using the NovaSeq SE50 strategy.