library_construction_protocol: In p16-CreER;R26-mTmG mice subjected to MI surgery, cardiac P16-positive cells were lineage-traced by administering tamoxifen via oral gavage on day 5 post-MI. At day 7, the infarcted region of the heart was dissected, enzymatically digested and lysed, followed by the removal of cardiomyocytes. Cells were then sorted by flow cytometry using F4/80 and PDGFRα antibodies to isolate distinct populations: GFPhiF4/80hi, GFPloF4/80hi, GFPhiPDGFRαhi and GFPloPDGFRαhi cells. Library preparation commenced with RNA quality control and reverse transcription into cDNA templates. Utilizing a transposase-mediated fragmentation and ligation strategy, cDNA molecules were prepared for sequencing. These molecules were then circularized to form single-stranded circular DNA. Subsequently, rolling circle amplification (RCA) was employed to generate DNA nanoballs (DNBs), each containing multiple copies of the template sequence. These DNBs were precisely loaded onto a proprietary patterned nanoarray chip using high-density nanoarray technology. Finally, sequencing-by-synthesis was performed employing combinatorial probe anchor ligation chemistry for high-throughput base detection and sequence determination.