library_construction_protocol: single cell capture using 10x genomics 3' mRNA library contruction from cells (v3) After the dissociation protocol, using the single cell 3' v3 protocol (10x Genomics), the cells were captured with the maximum target of 6000 cells.  During the capture the transcripts are first converted to cDNA and amplified.  The cell barcode and unique molecular identifier are added during the first strand synthesis of the double stranded cDNA.  The full length cDNA is then further processed to fragment the library to size for short read sequencing and amplified further to add the sequencing adapters and capture library index.