library_construction_protocol: The miRNeasy Mini Kit (217004; Qiagen, Valencia, CA, United States) was used for miRNA extraction from red muscle of gastrocnemius muslce. RNA sequence library preparation, sequencing, mapping, gene expression, and gene ontology enrichment analysis were performed by DNAFORM (Yokohama, Kanagawa, Japan). Qualities of total RNA were assessed by Bioanalyzer (Agilent) to ensure that RIN (RNA integrity number) is over 7.0. For library preparation, the QIAseq miRNA Library Kit (QIAGEN, 331502) was used according to the manufacturer's instructions.