library_construction_protocol: Dissociation was carried out with a Papain Dissociation System (Worthington Biochemical Corporation) but with a shorter papain incubation time to avoid cell death. Larval brains were initially incubated in papain mixture for 10 minutes at 34°C, followed by gentle trituration with a p1000 pipette to ensure uniform dissociation and a final incubation for 5 minutes. To avoid dissociation-induced activity and preserve hunger/feeding based neuronal activity patterns, actinomycin D was added at a concentration of 0.1 𝜇g/ml to the papain mixture to inhibit transcription. The cell suspension was filtered using a 40 μm cell strainer to avoid cell clumps. DAPI (1:1000) was added to the cell samples prior to sorting in a BD FACSAria II SORP cell sorter.GCaMP6s positive (LH) and negative (cells from all other brain regions, which we refer to as non-LH cells) cells were collected in Neurobasal medium with B27 supplementation and 10% FBS as they were prone to cell death. The proportion of GCaMP6s-positive LH cells is very low as compared to non-LH cells in the brain. Out of 100% live cells, 97% of cells were GCaMP6s-negative (non-LH cells) with only 3% being GCaMP6s- positive. Since the ratio of LH to non-LH cells is roughly 1:323, positive and negative populations were mixed at 50:50 ratio in both samples to ensure we captured a sufficient number of LH cells. Overall cell viability after mixing the positive and negative populations was 70-75%. Single cell 3' Gene Expression libraries were prepared using the 10X Genomics Chromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1 and Chromium Dual Index Plate TT Set A Sample Index following the manufacturer's instructions (10X Genomics, Pleasanton, CA, USA). The libraries were sequenced on an Illumina NovaSeq 6000 system (Illumina, San Diego, CA, USA) using indexed paired-end reads (2 × 151 bp), targeting a depth of approximately 50,000 reads per cell.