design: Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq
library_construction_protocol: Prior to use, cells were washed with PBS, dissociated with Trypsin-EDTA (0.05% for HEK293T and 0.25% for C3H10T1/2), resuspended with basic culture medium, filtered using 40 µm strainers and counted with Trypan blue live-dead stain using a Countess cell counter. Both HEK293T and C3H10T1/2 cells were maintained in basic culture medium (containing high-glucose DMEM with GlutaMax and pyruvate, 10% FBS and 1x Penicillin-Streptomycin). All cells were placed at 37 °C and 5% CO2 in a humidified incubator. Using Chromium Single Cell Expression 3' Reagent Kits Using Chromium Single Cell Expression 3' Reagent Kits