design: Ribo-depletion was performed with the RiboMinus Plant Kit for RNA-seq (ThermoFisher Scientific). The polyadenylation was carried out using E. coli poly(A) polymerase (New England Biolabs). The cDNA-PCR Barcoding Kit 24 V14 kit (SQK-PCB114.24, Oxford Nanopore Technologies) was used for library construction.