design: Genomic DNA was extracted from small tissue of adult Ascaris lumbricoides worm. The concentration and purity of genomic DNA was quantified using NanodropSpectrophotometer (Thermo Scientific; 2000). Library preparation was performed using QIASeq FX DNA Library Preparation protocol(Cat#180475) by following manufacturers instructions. The amplified library was purified using Cambrian Bioworks CamSelect NGS(#CBWD010) followed by library quality control check.