library_construction_protocol: Total RNA was extracted using a hot formamide lysis method. Cell pellets were resuspended in EDTA and formamide, incubated at elevated temperature to lyse cells, clarified by centrifugation, and RNA was purified using a silica column–based cleanup kit. Purified total RNA was subjected to bacterial rRNA depletion, followed by end-repaired using phosphatase and polynucleotide kinase treatments. Strand-specific RNA-seq libraries were prepared using the Collibri Stranded RNA Library Prep Kit for Illumina according to the manufacturer's protocol, followed by PCR amplification, size selection by gel extraction, and sequencing on an Illumina NextSeq 1000/2000.