library_construction_protocol: RNA isolation was performed using the RNeasy Micro Kit (Qiagen) according to the manufacturers instructions Barcoded mRNA-seq cDNA libraries were prepared from 100 ng of total RNA using NEBNext® Poly(A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manual with a final amplification of 12 PCR cycles. Quantity was assessed using Invitrogen's Qubit HS assay kit and library size was determined using Agilent's 2100 Bioanalyzer HS DNA assay.