design: Templated Oligo Sequencing (TempO-Seq) of FFPE Polyp Tissue from Patients in the Scottish Bowel Screening Program
library_construction_protocol: INCISE FFPE tissue full sections were cut to be 4μm thick and mounted on glass slides. To generate lysates, the full FFPE tissue was scraped off and placed in 1x FFPE Lysis Buffer (BioSpyder), and deparaffinised. Further processing included incubation in FFPE Protease (BioSpyder), followed by mechanical homogenization by trituration.  Detector oligo hybridization, digestion, and ligation, and subsequent PCR amplification was performed using a unique primer set for each sample, introducing a sample-specific barcode and Illumina adaptors according to manufacturer's instructions.  Barcoded PCR products was added to a flip-spin reservoir and purified using the Macherey-Nagel NucleoSpin Gel and PCR Cleanup Kit (Cat. 740609.50, Macherey-Nagel) and quantified by Qubit using the dsDNA kit (Cat. Q32851, ThermoFisher Scientific), to generate a single library.