library_construction_protocol: 2-10 testes from fetuses in the same litter from the same treatment group were pooled for each sample. GFP-positive and GFP-negative cells were separated by FACS. Total RNA was extracted from sorted cells using hte Arcturus PicoPure Kit per manufacturer's instructions, including DNase treatment. RNA libraries were prepared using the Illumina Nextera XT kit  for Illumina using manufacturer's instructions (Illumina, San Diego, CA, USA).