library_construction_protocol: After labelling, 10,000 labelled cells were collected for further reactions. For tagmentation, 50 ng of biotinylated DNA and 7.5 ul of the enzyme from Illumina Tagment DNA TDE1 enzyme and buffer kit were used. The transpositioned and amplified DNA samples were sequenced using NovaSeq 6000 with 1% PhiX spike-in generating paired-end reads.