design: The transcriptome sequencing on muscle tissue from control group (CTX) and denervated group(CTX-DeN)
library_construction_protocol: For RNA-seq analysis of mouse hindlimbs, total RNA was extracted from tissue samples firstly The RNA's quality and quantity were evaluated through spectrophotometry and gel electrophoresis. Next, the RNA was processed to generate a cDNA library, which was then subjected to high-throughput sequencing using a next-generation sequencing platform. The RNA's quality and quantity were evaluated through spectrophotometry and gel electrophoresis. Next, the RNA was processed to generate a cDNA library, which was then subjected to high-throughput sequencing using a next-generation sequencing platform.