library_construction_protocol: We used the CUTANA ChIC/CUT&RUN Kit (EpiCypher 14-1048) with antibodies against H2A.Z (Abcam ab4174), H3K4me3 (EpiCypher 14-1048), and IgG (EpiCypher 14-1048). Cells were dissociated using TrypLE, washed, and bound to 10 μl activated Concanavalin A-conjugated paramagnetic beads (EpiCypher), then resuspended with 0.5 μg of the antibody of interest and incubated overnight at 4°C on a rotator. The next day, the beads were washed twice with permeabilization buffer and incubated with 2.5 μl pAG-MNase (Epicypher) for 10 minutes at room temperature. Following binding, the beads were washed, and 2mM CaCl2 was added to begin digestion. Digestion was allowed to proceed for 2 hours at 4°C, then the reaction was terminated by adding 33 μl Stop Buffer (Epicypher) and incubating the reactions at 37°C for 10 minutes. We included a 0.5 ng E. coli DNA (Epicypher 18-1401) spike-in. DNA was purified with bead cleanup provided (EpiCypher). Libraries were then prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs E7645S), pooled, and sequenced on an Illumina NovaSeq S1 6000 v1.5 with 2 x 90 bp paired-end reads.