design: RNA was extracted using the QIAGEN PAXgene Blood RNA Kit according to the manufacturer's instructions. Total RNA was used as input material for the RNA sample preparations. rRNA was removed from total RNA by using TIANSeq rRNA Depletion Kit (Animal). First strand cDNA was synthesized using random hexamer primer and reverse transcriptase, followed by the synthesis of the second strand by adding buffer and dNTPs (with dTTP replaced by dUTP). The synthesized double-stranded cDNA was treated by terminal repair, A-tailing, adaptor ligation, and PCR enrichment after fragment selection. The strand-specific library was obtained by selecting cDNA fragments of 370-420bp by purification with AMPure XP beads. The qualified library was pooled and sequenced on the Illumina NovaSeq X Plus platform with a 150  2 paired-end configuration.