library_construction_protocol: Novogene's RNA detection for RNA samples primarily includes four methods: 1.Agarose gel electrophoresis to analyze the degree of RNA degradation and to check for contamination. 2.Nanodrop to assess the purity of RNA (OD260/280 ratio). 3.Qubit for precise quantification of RNA concentration. 4.Agilent 2100 for accurate assessment of RNA integrity. The Iso-Seq Express 2.0 kit was used to synthesize cDNA using QC-passed RNA. This was followed by library construction using the Kinnex Full-Length RNA Kit.