design: RNA-Seq of zebrafish embryos (96hpf) treated with different concentrations of Triiodothyronine (T3) against untreated control groups
library_construction_protocol: at the end of exposure, 10 Embryos were euthanized on ice then homogenised in lysis buffer using Lysing Matrix D ceramic beads in FastPrep-24 homogeniser (MP Biomedicals, Irvine, USA) at 5 m/s speed for 45 seconds according to the RNA extraction protocol provided by the manufacturer (NucleoSpin RNA Protein kit; Macherey-Nagel 740933.250). Zebrafish embryos were exposed to T3 for 96 hours according to OECD test guidelines No. 236 at 26 ± 1 °C and a light/dark cycle of 14:10 hours. three zebrafish embryos replicate groups originating from three different tanks were exposed to 33 µg/L (mid exposure concentration) of T3 (3, 3′,5-Triiodo-L-thyronine, CAS 6893-02-3) in addition to untreated control groups.  At the end of exposure time (96 hours), RNA was extracted from 10 embryos using a NucleoSpin RNA/Protein kit (Macherey-Nagel, Düren, Germany). libraries were prepared at NGS-Services for Integrative Genomics at the University of Göttingen in Germany. For each 100 ng/µL sample, mRNA was purified through poly A selection followed by cDNA libraries preparation using the TruSeq RNA Library Prep Kit v2 (Illumina).