library_construction_protocol: Total RNA isolation was performed using the RNeasy Mini Kit (Qiagen), according to the manufacturer's instructions Library construction and stranded mRNA sequencing were conducted at Novogene. Briefly, quality control for RNA samples was performed using Nanodrop and agarose gel electrophoresis. Next, messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. The enriched mRNA was fragmented randomly by adding fragmentation buffer, then the cDNA was synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I were added to initiate the second-strand synthesis. After a series of end terminal repair, A tailing, sequencing adapter ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment. Quality control for the library was performed using Qubit fluorometer and quantitative real time-PCR for quantification and Agilent Bioanalyzer 2100 for size distribution detection. Quantified libraries were pooled and sequenced on the Illumina NovaSeq 6000 (S4 flowcell), using a paired-end 150bp (PE150) sequencing strategy. The clustering of the index coded samples was performed according to the manufacturer's instructions.