design: Genome DNA was extracted from ovaries of 5 day-old flies, ultrasonicated, and used for library preparation with NEBNext Ultra II DNA library preparation kit (NEB) according to manufacturer recommendations. The libraries were sequenced on NovaSeq 6000 (Illumina) at the Resource Center of the NRC Kurchatov Institute. In total, 11-15 million of PE reads with 180-210-bp fragment sizes and 12-19-fold coverage of the euchromatic genome were obtained. The reads were mapped to the dm6 genome assembly with bowtie2, deduplicated, and single-mapped reads were counted on 2 kb bins.