design: Maturation of iPSC-derived Hepatocyte-Like Cells with 5 nM T3
library_construction_protocol: On day 28 of differentiation, the cell culture medium was discarded, and cells were transferred and stored at -80 °C. On the day of RNA extraction, plates were thawed on ice, lysis buffer was added to the culture wells, and the surface was scraped to maximize lysate yield. RNA from samples was extracted with Aurum™ Total RNA Mini Kit (Bio-Rad) according to manufacturer's instructions. Bulk RNA-seq libraries were generated using Poly(A) mRNA selection and prepared with the NEBNext Ultra II RNA Library Prep Kit for Illumina, following the manufacturer's instructions.