design: Hep53.4 tumors were harvested 14 days after implantation, dissociated into single-cell suspensions as described above, and stained with anti-CD45 and Live/Dead dye. Live CD45+ cells were subsequently sorted using a BD FACS system. Sorted cells were subsequently barcoded using a Chromium Controller (10x Genomics), and cDNA libraries were prepared using the Chromium Single Cell 30 v2 Reagent Kit (10x Genomics, Cat# PN-120267) according to the manufacturers protocol. Libraries were sequenced on an Illumina HiSeq platform.