library_construction_protocol: At least 10 – 20 mg of frozen tissue was obtained from each sample using a metallic block and hammer to take a portion of the dissected cortex tissue. The tissue was then sealed inside of a Covaris Tissue TUBE (Covaris, cat. Nos. 520021 and 520023) and thoroughly pulverized using the chilled hammer and metallic block, with intermittent submerging into liquid nitrogen to maintain cold temperature and brittleness. Using a clean, pre-chilled scoopula, pulverized tissue was then transferred to a pre-chilled 2 mL DNA LoBind tube. RNA was isolated using the miRNeasy Mini Kit according to manufacturer instructions (QIAGEN, cat. no. ​217084). RIN scores were calculated by a 2100 BioAnalzyer (Agilent) using the RNA 6000 Nano Kit following manufacturer instructions (Agilent cat. no. 50671511) . Transposition, nuclei isolation, barcoding, and library preparation were performed according to the 10X Genomics Chromium Next GEM Single Cell Multiome protocol CG000338 Rev E with the following alterations. Each sample was loaded on one lane of the Chromium Next GEM Chip J. Nuclei were loaded according to manufacturer's recommendations to target recovery of 10,000 nuclei per lane.