design: For Oxford Nanopore sequencing, the genomic DNA of the BY0012 subclone 1 was isolated with the Quick-DNA High Molecular Weight (HMW) MagBead Kit (Zymo Research, Irvine, CA, USA) according to the company's instructions, except that cell lysis was not performed with lysozyme, but with R-Zymolyase (RNase-A containing zymolase; Zymo Research, Irvine, CA, USA). Library construction was performed with Ligation Sequencing Kit (SQK LSK109; Oxford Nanopore, Oxford, UK) and sequenced on a MinION R9.4.1 Flow Cell with approximately 500x coverage calculated for the S288c genome).