library_construction_protocol: Gut, nephridia, calciferous gland, crop, gizzard, seminal vesicles, clitellum, nerve cord, body wall, and pharynx tissues were dissected and the RNA was extracted using Trizol reagent and homogenisation with Qiagen Tissuelyser with a 5mm stainless steel ball The SMRTbell Express Template Prep Kit 3.0 was used to prepare the HiFi SMRTbell Library of the Cwmystwyth worm RNA with PacBio barcoding primers. The library sequencing was performed on a PacBio Sequel IIe system (Pacific Biosciences) on a single 8M cell.