library_construction_protocol: Twenty-four hours post-surgery, all mice were euthanized and lung tissues were swiftly excised. Under sterile conditions, tissues were washed twice with ice-cold PBS supplemented with 0.04% BSA. Using sterile surgical scissors, the clean tissues were carefully minced into approximately 0.5 mm3 fragments and then placed in freshly prepared digestion solution. The digestion was conducted at 37°C for 30 minutes, with intermittent stirring every 10 minutes. The resulting cell suspension was filtered twice through a BD 70 μm cell strainer, followed by centrifugation at 4°C at 400 g for 5 minutes. The pellet was resuspended in an appropriate volume of medium, mixed with an equal volume of red blood cell lysis buffer, and incubated at 4°C for 5 minutes. Afterward, the sample was centrifuged at 400 g for 5 minutes, and the supernatant was discarded. The pellet was washed once with medium and centrifuged again at 400 g for 5 minutes. After discarding the supernatant, the sample was resuspended in 100 μl of medium. The freshly prepared single-cell suspension was adjusted to a concentration of 30,000 cells per sample. Library construction followed the manufacturer's instructions for the 10x Genomics Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (Catalog No. 000388).