design: Total RNA was extracted from MCF7 and T47D human breast cancer cell lines subjected to either non-silencing control (shNS) or progesterone receptor knockdown (shPR). PolyA+ mRNA was enriched and strand-specific cDNA libraries were prepared using a standard mRNA-seq library preparation kit. Libraries were sequenced on an Illumina NovaSeq platform to generate 150 bp paired-end reads, with two biological replicates per condition.