design: Muscle tissue sample was homogenized in a tissue grinder for total RNA extraction using TRIzol (Invitrogen, USA) extraction method. The purity of the total RNA was estimated using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA). The integrity and concentration of total RNA was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). 54 rRNA-depleted and random priming RNA-seq libraries were constructed. And then these libraries were sequenced using MGI DNBSEQ platform with a paired-end sequencing length of 150 bp (PE 152) by Annoroad Gene Technology Co., Ltd (Beijing, China).