library_construction_protocol: DNA was extracted from the conjunctival swab with a DNeasy PowerSoil Kit (Mobio, Carlsbad, CA, USA) and the DNA concentration and purity were measured with NanoDrop One (Thermo Fisher Scientific, MA, USA). DNA fragmentation was performed using S220 Focused-ultrasonicators (Covaris, USA), and purification was performed by Agencourt AMPure XP beads (Beckman Coulter Co., USA). Library construction was performed utilizing the TruSeq Nano DNA LT Sample Preparation Kit (Illumina, San Diego, CA, USA). PCR cycles used were 16 for library construction.