library_construction_protocol: This protocol involves extracting RNA from high-lipid tissue using an automated homogenization method followed by a filtration column. The process involves grinding the tissue into fine powder in liquid nitrogen, adding a lysis solution to the tissue powder, vortexing, and centrifugation to isolate the lysate. The RNA is then purified and concentrated using a filtration column, followed by a series of washing steps. Low-input eukaryotic mRNA library preparation (non-directional)