library_construction_protocol: RNA was harvested using Rneasy mini plus kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries. For LF1 samples, Total RNA was extracted from early passage, SW-induced quiescent, early and late senescent cells. Cells were washed with PBS and harvested using Trizol reagent (Invitrogen), after which Total RNA was harvested using the Qiagen Rneasy kit. Libraries were constructed by Azenta. For LF1 samples, NEBNext ultra II directional RNA library prep kit for Illumina along with the NEBNext Poly(A) mRNA magnetic bead isolation module was used to make isolated mRNA and cDNA libraries. Strand specific cDNA libraries were sent to Azenta for paired-end sequencing