library_construction_protocol: Sequencing libraries for transcriptome analysis were prepared according to Illumina's kit. Poly (A) mRNA was purified from total RNA using oligo(dT) magnetic beads and then fragmented using the RNA Fragmentation Kit. Six random hexamer primers and short fragment templates were used to synthesize the first strand cDNA. Second strand cDNA is synthesized using buffer, dNTPs, RNase H and DNA polymerase I and then ligated to the sequencing adapter. Suitable cDNA fragments are separated by gel electrophoresis analysis and PCR enrichment is performed, and the final product is loaded onto the Illumina HiSeq 2000 platform for transcriptome sequencing. Total reads of low quality and adapter sequences were trimmed using Illumina Pipeline. Transcripts were functionally annotated by comparing sequenced reads to the Prunus persica genome.