library_construction_protocol: Total RNA was extracted using the Direct-zol RNA Miniprep Plus Kit (Zymo Research, R2072) according to the manufacturer's instructions. The purified RNA samples were then submitted to Novogene Corporation Inc.  (Sacramento, CA, USA) for RNA-Seq. Quality control was performed using the Agilent 5400 Fragment Analyzer System to evaluate RNA quantity, purity, and integrity. RNA libraries were prepared using poly(A) enrichment, and sequencing was carried out on the Illumina NovaSeq X Plus platform with a 150 bp paired-end strategy. RNA libraries were prepared using poly(A) enrichment, and sequencing was carried out on the Illumina NovaSeq X Plus platform with a 150 bp paired-end strategy.