design: Artificially bred Mytilus edulis spat (shell length 1-2 cm) was set as Group DM Replicate 1 and exposed to the optimal concentration of antifouling agent. Its soft tissue was collected at 12 h post-exposure and stored in liquid nitrogen. Total RNA was extracted from the soft tissue using FreeZol Reagent. mRNA was enriched, reverse-transcribed into cDNA, and subjected to end repair, adapter ligation and 15-cycle PCR to construct the RNA-Seq library, aiming to identify differentially expressed genes related to attachment inhibition under antifouling agent exposure (refer to related documents).