library_construction_protocol: Total RNA was extracted using the Total RNA Extractor（Trizol）kit (B511311, Sangon, China) according to the manufacturer's protocol.A total of 2μg total RNA per sample was used as input material for small RNA library preparations. Sequencing libraries were generated using NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (NEB, USA.) following manufacturer's recommendations.