design: Tissues were subject to RNA extraction, along with the guidelines of TRIzol reagent (TaKaRa, Dalian, Liaoning, China). The quality and integrity of purified RNA samples were estimated through a combination of agarose gel (1%, w/v), NanoDrop ND-1000, Qubit and Agilent 21000. The samples were subject to the construction of cDNA libraries with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, USA). Libraries were sequenced on an Illumina NovaSeq 6000 sequencing system, with 2150 bp paired-end protocols.