library_construction_protocol: 72 hours after silencing cells were collected and RNA extracted using the RNeasy Plus Kits for RNA Isolation (QIAGEN, ID: 74134) and quantified by Nanodrop. Libraries of cDNA were prepared with TruSeq mRNA Library Prep Kit (Illumina) and sequenced using the Novaseq instrument (Illumina) 100 bp single-end up to 30 million reads.