library_construction_protocol: DLPFC tissue blocks were dissected from fresh-frozen postmortem human brain tissue slabs. Coronal cryosections (10 µm thick) were mounted onto 10x Genomics Visium Spatial Transcriptomics arrays (v1, 3' Gene Expression based on 3' poly(A) capture-based chemistry). Sections were immunostained for nuclei (DAPI), neurons (NeuN), perineuronal nets (WFA), and vasculature (Claudin-5), and subsequently processed for spatial transcriptomics. Tissue permeabilization and cDNA synthesis incorporating positional barcodes were performed according to the manufacturer's instructions (CG000239 Rev F). Libraries were prepared using the Visium Spatial Gene Expression slides & kit according to manufacturer's instructions (CG000239 Rev F)