library_construction_protocol: Total rRNA is depleted then fragmented and primed for cDNA synthesis. Fragmented RNA was reverse transcribed into first strand cDNA using random primers. The RNA template was removed and replaced with a complementary strand incorporating dUTP (instead of dTTP), resulting in double-stranded cDNA (ds cDNA). RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) with 100ng RNA