library_construction_protocol: Total RNA was extracted using the Qiagen RNeasy kit (Qiagen, 74004). Samples were spiked-in using ERCC RNA Spike-In Mix (Thermo, 4456740) and 500ng RNA was used as input for library preparacion. Library preparation was performed using KAPA RNA HyperPrep Kit with RiboErase (Roche, 8098131702) following manufacturer's instructions.