library_construction_protocol: Total RNA extraction from plant material subjected to the low-temperature treatments (0ºC, 4ºC, and 10ºC) and as control (22ºC) for 24h was conducted following the ConcertTM Plant RNA Reagent protocol (Invitrogen Life Technologies, Waltham, Massachusetts, USA). The quality of the RNA samples was evaluated using a 1% agarose gel and 0.5X TBE buffer. RNA concentration was determined using a NanoDrop ND-1000 UV–vis spectrophotometer (NanoDrop Technologies Inc., Wilmington, DE, USA). Each set of samples was analyzed in triplicate. Subsequently, the RNA samples were prepared for shipment to the GENEWIZ company (Burlington, MA, USA) for paired-end RNA sequencing, following the RNAstable® protocol for RNA preservation at room temperature. Sequencing was performed with HiSeq 2500 technology (Illumina) in rapid mode with v2 reagents. The readings produced were 250 bp reads per end, according to the manufacturer's instructions.