library_construction_protocol: Total RNA was extracted from the tissue using TRIzol® Reagent according the manufacturer's instructions.Then RNA quality was determined by 5300 Bioanalyser (Agilent) and quantified using the ND-2000 (NanoDrop Technologies).Only high-quality RNA sample [OD260/280=1.8~2.2,OD260/230>2.0,RQN>6.5,28S:18S21.0, >1ug OR OD260/280-1.8-2.2, RIN>6.5,>10ng ]was used to construct sequencing library. The sequencing library was performed on NovaSeq X Plus platform(PE150) using NovaSeqReagent Kit.