design: Extract total DNA from the collected samples using commercial kits or customized protocols.Perform PCR to amplify the target regions. Add barcode sequences and platform-specific adapters during this step. Library Normalization & Pooling: Normalize the concentration of individual PCR products and pool them together to ensure even representation. Conduct quality checks on the pooled library using gel electrophoresis, qPCR, and/or bioanalyzer to assess size distribution and concentration. Sequence the prepared libraries on high-throughput platforms.