library_construction_protocol: RNA was harvested and extracted using the RNAeasy Kit (Qiagen) RNA quality was analyzed using the Agilent Tapestation 4200, and only samples with an RNA Integrity Number (RIN) exceeding 8.0 were selected for library construction. Libraries were prepared using the Illumina Stranded mRNA Sample Prep Kit with Illumina RNA UD Indexes (Illumina, San Diego, CA), following the manufacturer's guidelines.