library_construction_protocol: RNA was extracted from cell pellets using the RNeasy Kit (Qiagen). RNA concentration was determined with a Nanodrop spectrophotometer. Library preparation was performed using the TruSeq Stranded RNA kit (Illumina) as per manufactures instruction followed by paired-end sequencing on a NovaSeq 6000 (Illumina) at the German Cancer Research Center (DKFZ).