library_construction_protocol: RNA was extracted using the RNeasy Mini Kit (Qiagen, 74106) following the manufacturer's instructions. RNA was quantified by Nanodrop and Qubit RNA High Sensitivity kit. 100 ng of RNA per sample was treated for ribosomal RNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs, E6350), followed immediately by Library prep using the NEBNext Ultra II kit (New England Biolabs, E7770).