design: Following RNA isolation, cDNA synthesis was carried out. The cDNAs were converted into double-stranded cDNAs and subjected to end repair, A-tailing, and adapter ligation. For the construction of lncRNA and mRNA sequencing libraries, the TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) was used, and for miRNA sequencing libraries, the TruSeq Small RNA Sample Preparation Kit (Illumina, San Diego, CA, USA) was employed [26]. A total of 8 PCR cycles were applied to 1 l of the library product to generate a sufficient quantity and quality of the library for sequencing. To verify the absence of primers and adapter dimers, the libraries were analyzed using a BioAnalyzer device. The concentration of the libraries was validated by performing qPCR reactions using the KAPA SYBR FAST Master Mix Universal kit.