design: BBM DR.Gimap4-Gimap5-/Gimap4-Gimap5- Rat (sBBM 6 3:6) (BBDP.BBDR-(Zfp786-Aoc1)/Ulund, RRID:RGD_597830140): Illumina NovaSeq 6000 whole genome sequencing to identify candidate diabetes genes
library_construction_protocol: DNA from one individual of the Rattus Norvegicus BioBreeding (BB) Diabetes Resistant (DR) sBBM line at Clinical Research Centre (CRC) at Lund University (Malmö, Sweden) was used to prepare two libraries for whole genome sequencing at SNP&SEQ/NGI, SciLifeLab (Uppsala, Sweden). Each library was prepared from 1μg DNA using the TruSeq PCR free DNA sample preparation kit (cat#20015962, Illumina Inc.) and unique dual indexes (cat#20022370, Illumina Inc.), targeting an insert size of 350bp. The library preparation was performed according to the manufacturers' instructions (guide#1000000039279). Paired-end sequencing with 150bp read-length was performed with a S4 flowcell on the NovaSeq 6000 system and using v1.5 sequencing chemistry (Illumina Inc.). A sequencing library for the phage PhiX was included as 1% spike-in in the sequencing run.