library_construction_protocol: NPCs were grown to confluency and dissociated with 1:1 accutase:PBS (Invitrogen, #A1110501). Cells were filtered for debris and non-dissociated aggregates using 30 um filters (Sysmex, #04-0042326). Cells were encapsulated into lipid droplets using a 10X Chromium Controller (NeuroTechnology Studio, BWH). Synthesis of cDNA was performed, followed by library preparation and sequencing (Harvard Biopolymers Facility).