design: For RNA extraction, the frozen pellet was resuspended in 1.5mL PureZol (Cat. #7326890, Bio-Rad, USA), incubated 5min at room temperature, then mixed with 300L chloroform and incubated 15min with occasional inversion. After centrifugation (12,000 rcf, 15min, 4C), 650L of the upper phase was transferred to a fresh RNase-free tube, mixed with an equal volume of 75% ethanol, and processed using the Qiagen RNeasy Mini Kit (74106, QIAGEN, Netherlands) per manufacturers instructions. RNA quantity was measured with a NanoPhotometer N50/N60 (Techno Scientific, USA); purity and degradation were checked on a 1% agarose gel. Libraries were prepared with the NEBNext Ultra II RNA Library Prep Kit (E7770L, NEB, USA), quality-checked on a Fragment Analyzer, and sequenced on an Illumina NovaSeq 6000.