library_construction_protocol: MCF-7 cells were transfected with TRIM28, CtBP1/2, Ctrl(NC) siRNA for 2days using Lipofectamin 3000 Transfection Reagent according to the product manual. RNA was extracted using the RNEasy Plus Micro kit (Qiagen, 74034). 1μg of total RNA was used to prepare libarary construction. Preparation of RNA library and transcriptome sequencing was conducted by Novogene Co., LTD (Beijing, China) using standard Illumina protocols.