design: Library preparation and sequencing were performed by Sangon Biotech (Shanghai, China) and GENEWIZ (Suzhou, China). PCR amplicons underwent end repair, 5 phosphorylation, and dA-tailing in a single reaction, followed by T-A ligation to attach sequencing adaptors to both ends. A secondary PCR was carried out using P5 and P7 primers to introduce unique index sequences and flow cell-binding sites for multiplexed sequencing. Final libraries were purified with magnetic beads and quality-checked prior to sequencing. Paired-end sequencing (150 bp or 300 bp) was conducted on either the DNBSEQ-G99 platform (MGI, Shenzhen, China) or the NovaSeq 6000 platform (Illumina, CA, USA).