design: DNA was extracted from muscle tissue using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany)/NEB Monarch Genomic DNA Purification Kit  including RNase A treatment. DNA was sent for purification (Vahtstm DNA Clean Beads; Vazyme Biotech, Nanjing, China), PCR-free library preparation (NEBNext Ultra II FS DNA PCR-free Library Prep Kit for Illumina; NEB) and subsequent paired-end sequencing on a NovaSeq 6000 (Illumina, San Diego, USA) using the NovaSeq 6000 S4 Reagent Kit (Illumina) to Biomarker Technologies (bmkgene; Beijing, China).