design: RNA-seq of PHACTR1 knockdown and overexpression in HT1080 stable cell lines
library_construction_protocol: All cell samples (n=6/cell line) were generated in parallel using the same passage cells, tissue culture reagents and culture conditions followed by concurrent harvesting. Cells were plated 24 hours prior to harvest at 2.2 x 105 cells/mL in DMEM with 10% FBS and 0.2 μg/mL puromycin for stable cell lines.  Cells were washed once with ice-cold PBS and RNA was extracted from cells using RNeasy Mini Kit (QIAGEN, #74104), as per the manufacturer's instructions.  RNA libraries were prepared using RNA with RIN score >9.8 using the KAPA Total RNA Library Preparation kit (Roche, #08098093702).