library_construction_protocol: 40 mL of culture were harvested at mid-exponential phase (OD600 of 0.5-1 for all conditions) by centrifugation at 4°C at 2000 x g for 10 minutes. Total RNA was extracted using the Qiagen RNeasy Kit and quantified. rRNA depletion using the Ribo-Zero RNA Plus rRNA Depletion Kit (Illumina) was performed by SeqCoast Genomics. Samples were prepared for whole genome sequencing using an Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus Microbiome and unique dual indexes by SeqCoast Genomics. Sequencing was performed by SeqCoast Genomics on the Illumina NextSeq2000 platform using a 300 cycle flow cell kit to produce 2x150bp paired reads.