design: Every sample with two biological replicates. Briefly, 3 g of 35S:RDE-GFP, 35S:DPa-GFP and 35S:GFP transgenic plants grown for 10 days were cross-linked with 1% formaldehyde and ground into powder in liquid nitrogen. After ultrasound, DNA-protein complex was incubated with GFP antibody (ab290, ab290). The immunoprecipitated complex was washed, and DNA was extracted and purified by MinElute PCR Purification Kit (QIAGEN, #28006). The ChIP-Seq library was prepared using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme, #TD501) according to the manufacturer s instructions. For ChIP-seq, extracted DNA was ligated to specific adaptors followed by deep sequencing in the Illumina Novaseq 6000 using 150bp paired-end reads.