design: DNA was extracted from soil using the PowerSoil Pro Kit (Qiagen, Denmark). Amplicon metagenomic sequencing targeted the V3-V4 hypervariable region of the 16S rRNA gene using KAPA HiFi HotStart ReadyMix (KAPA) and the primer set 341F (5'  CCTACGGGNGGCWGCAG  3') and 805R (5'  GACTACHVGGGTATCTAATCC  3'). However, reverse reads were removed from the dataset in quality filtering due to lack of amplification and/or low quality scores. No template controls were included in each batch run. DNA was purified using Mag-Bind TotalPure NGS at 0.8 (Omega Bio-tek, GA, USA), and all other library preparation followed the Illuminas Metagenomic 16S rRNA guide (Part # 15044223 Rev. B); final purified DNA products were sequenced on an Illumina MiSeq platform using the MiSeq Reagent Kit v3 (600-cycle) (Illumina, San Diego, CA, USA).