library_construction_protocol: RNA samples were extracted using RNAeasy plus kit (Qiagen) and sent to School of Biomedical Engineering sequencing core at UBC for quality control, library preps and sequencing. Qualifying samples were prepped using Illumina Stranded mRNA prep with standard protocol. Paired-end equencing was performed on Illumina NextSeq2000 (59bp x 59bp reads), with a read depth of 30 million reads per sample.