design: DNA was extracted with Quick-DNA Fecal/Soil microbe miniprep kit. DNA for metagenomics analysis was quantified by Qubit (Thermo Fisher). 150ng DNA was used as input into the Illumina DNA Prep kit (Illumina) for library preparation following manufacturers instructions. Libraries were pooled for sequencing on a NextSeq2000 instrument targeting 30M, paired-end 150bp reads per sample.