library_construction_protocol: After 2 or 6 h, the culture supernatant was removed and cells were washed in triplicate with sterile dPBS. Total RNA was prepared from treated and untreated A549 cells using the RNeasy Kit (Qiagen, Hilden, Germany). The samples were stored at -80°C. RNA libraries for RNA-seq were prepared using Illumina Stranded Total RNA Prep following manufacturer's protocols