design: DNA isolation from a 14-day-old kombucha liquid was carried out with the DNeasy PowerSoil Pro kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, The extracted DNA was purified using NucleoMag NGS Clean-up and Size Select. Bacterial sequencing analysis was performed with QIIME 2 2020.6 (Bolyen et al., 2019) adapted for Illumina data. Raw sequence data were quality filtered using the q2-demux plugin followed by denoising with DADA2 (Callahan et al., 2016)  (via q2-dada2). Taxonomy was assigned to amplicon sequence variants (ASVs) using the q2-feature-classifier described by Bokulich et al. (2018)  against the Greengenes 13_8 99 % OTUs reference sequences.