library_construction_protocol: Tissue was transported in DMEM with 10% FBS to preserve stability at room temperature to preserve temperature sensitive granulocytes. Tissue was minced and digested in two rounds (20 minutes and 15 minutes) in DMEM without FBS using Liberase TM (Sigma) in a 37 °C water bath with gentle manual inversion. The digested tissue was resuspended in FACs buffer passed through a 70 uM strainer (Corning), centrifuged and subjected to CD45 enrichment using the Miltenyi biotech CD45 microbeads as per the manufacturer's instructions. In parallel, 1 ml of blood from blood isolated in EDTA coated tubes was also processed to isolate leukocytes using whole blood lysis protocol recommended by 10X Genomics (CG000392) using eBioscience 1X RBC Lysis Buffer (Thermo fisher) with PBS washes and low speed centrifugation To combine the matched tissue and blood samples to load on a single BD Rhapsody cartridge, the processed matched tissue and blood samples were labeled with sample tags using the BD Single-cell Multiplexing kit in accordance with the manufacturer's protocol. The individual samples were resuspended in BD Rhapsody sample buffer (BD Biosciences) and stained with DRAQ7 and Calcein AM florescent dyes to enable visualization for counting and calculation of quality control metrics in the BD Rhapsody single cell system.  The cells were counted, and blood-tissue samples mixed, passed through a 70 micron strainer and up to 10,000 cells loaded in the BD Rhapsody microwell cartridge system. Magnetic capture beads were then loaded close to saturation so that each microwell contains one magnetic bead.  Each of the cells were isolated in the microwells, lysed and the polyadenylated mRNA molecules from each cell captured by individual magnetic capture beads containing unique molecular identifiers (UMIs) and cell specific barcodes. The capture beads were retrieved from the BD Rhapsody single cell system and cDNA reverse transcription carried out with cDNA with UMI and cell specific barcode information added to the cDNA molecules. 3' end whole transcriptome amplification (WTA), sample tag libraries were prepared according to the BD Rhapsody WTA and sample tag preparation protocol. For quantification and quality control purposes the libraries were subjected to Qubit and Tape station analyses. For calculation of total paired end reads, sequencing depth of 60,000 reads per cell were used for the WTA library analyses, for the sample tag libraries sequencing depth 1200 reads per cell. The WTA and sample tag libraries were mixed according to custom BD Rhapsody calculations.