library_construction_protocol: Total RNA was extracted using the TRIzol method, and the RNA was resuspended in DNase/Rnase free water. The samples were subsequently sent to Novogene for sequencing using the Illumina NovaSeq 6000 platform. The FASTQ files were returned to us from the sequencing company. According to the manufacturer's quality control report, ribosomal RNA was removed from total RNA, followed by ethanol precipitation. After fragmentation, the first strand cDNA was synthesised using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, enzyme digestion, amplification and purification.