library_construction_protocol: RNA was harvested using Trizol reagent. 1 ug of total RNA for the construction of sequencing libraries. total RNA samples underwent oligo dT enrichment (rRNA removal), followed by library preparation using the KAPA Stranded RNA-Seq Library Prep Kit (Roche). During library construction, double-stranded cDNA synthesis employed the dUTP method in combination with subsequent high-fidelity PCR polymerase, ensuring that the final RNA sequencing library is strand-specific. The constructed libraries were assessed for quality using the Agilent 2100 Bioanalyzer and quantified via qPCR. The pooled libraries from different samples were sequenced on the Illumina NovaSeq 6000 sequencing platform, which included steps such as NaOH alkaline single-strand generation, in-situ amplification on the Illumina flow cell, and 150-cycle paired-end sequencing.