library_construction_protocol: The aortae were cut into small pieces and moved into a new dish containing enzyme solution, incubated at 37°C in the incubator for about 1 hr. After an hour, the plates were washed with 2 mL warmed DMEM medium (DMEM + 5% FBS + P/S). Cells were collected by spinning 1500 rpm for 5 mins. The supernatant was carefully removed and 0.5 mL sterile PBS containing 1% BSA was added. Single cell of mouse arterial specimens was prepared as above. The cell viability exceeded 90% and was determined under the microscope with trypan blue staining. 20 μL of cell suspension was calculated to contain ~20,000 cells for each sample. Single-cell capturing and library construction were performed using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10× Genomics) according to the manufacturer's instructions.