design: DNA was extracted from ethanol-preserved fin clips using either DNeasy Tissue Kits (Qiagen, Inc.) or AgenCourt DNAadvance kits (Beckman-Coulter, Inc).  A minimum of 200ng of DNA in 50ul of H20 was used per specimen for Illumina library preparation.   Library preparation and sequencing  steps were performed by the Michigan State University Research Technology Support Facility (RTSF) Genomics Core or Genewiz (Azenta Life Sciences, Inc) .  Libraries were either prepared using a Rubicon Genomics ThruPLEX DNA-Seq library preparation kit or Illumina TruSeq Nano DNA Library preparation kit following manufacturers instructions.  Following QC, pooled libraries were loaded for sequencing on either an Illumina HiSeq 2500 (high output mode) or  Illumina HiSeq 4000 in either 2x125bp or 2x150bp output mode correspondingly.  Base calling was done by Illumina Real Time Analysis (RTA) and output of RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq.