design: RNA samples were quantified using a Qubit 4.0 fluorometer and assessed for quality with an Agilent 2100 Bioanalyzer. cDNA libraries were prepared using the Illumina TruSeq Stranded Total RNA Prep kit (with Ribo-Zero Plus) and an indexing kit. The quantity and quality of cDNA libraries were similarly assessed. Experimental and control group libraries were pooled and sequenced on an Illumina NovaSeq 6000 system using a 150bp paired-end dual-indexed method. Sequencing quality was evaluated with FastQC, and low-quality reads and adapter sequences were trimmed using Fastp. Trimmed reads were aligned to the GRCh38/hg38 human reference genome with the STAR package, and differential expression analysis was conducted using DESeq2.