design: Single-cell omics sequencing of kidney marrow cells from heterozygous gata2b mutated and wild type zebrafish
library_construction_protocol: Adult zebrafish were euthanized, kidney marrow was isolated and dissociated by pipetting in PBS/10% FCS. 7-AAD (7-amino-actinomycin D) 0.5mg/L (BD biosciences) was used for live/dead discrimination. Sorting was performed using FACSAriaIII (BD). 70.000 single viable cells were sorted from 2 pooled kidney marrow of female Tg(CD41:GFP) zebrafish and supplemented with 114-1607 CD41:GFPlow expressing cells. cDNA was prepared using the manufacturers protocol (10x Chromium V3). Library was prepared using the manufacturers protocol (10x Chromium V3).