library_construction_protocol: For each timepoint, two independent isolation protocols were used for D0, D3, D5, D9, D19 and D25. A gradient centrifugation was used to enrich cell types of interest and cells were magnetically enriched when feasible. In protocol A the following cell types were FACS sorted at equal ratios: alive singlet, Cd45+, Lyve-1+, Pdpn+, Epcam+, Cd31+ Lyve-1-, retinoid-autofluorescent cells (405 nm excitation, 450/40 emission, according to Mederacke et al. Nat. Methods (2015). For protocol B the following cell types were FACS sorted at equal ratios: alive cells from HSC prep, Cd45+ Itgax+ Cd64-, Epcam+, Cd3e+ Lyve-1-.  For the diet stop condition and Mgl2-DTR cell depletion protocol B was used and compared with protocol B data of the respective timepoint. Libraries from protocol A derived data were generated using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Single Index), while libraries from protocol B data were generated using Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index).