library_construction_protocol: Total RNA was extracted from L. braziliensis cultures using the Directzol kit Quick-RNA Miniprep (Zymo Research) according to the manufacturer's instruction. After extraction, RNA samples were treated with TURBO™ DNAse to remove any DNA remains. The libraries were prepared (Illumina Ribo-Zero plus rRNA Depletion & TruSeq Stranded Total RNA Library Prep Gold Kit) and then proceeded to sequencing (Illumina platform), generating approximately 40 million short (101bp), paired and strand-specific reads per library.