design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 1420 day-old seedlings (from Hazera company), PEG-mediated CRISPRCas9 RNP transformation, UMI-DSBseq amplicon library targeting chr11;58161684;58161704;+_1st_rep (target sequence: TCTTCAATATCTAGATATTGAGG) prepared for paired-end Illumina sequencing