library_construction_protocol: RNA was isolated using Trizol (Invitrogen 15596-026) Libraries were prepared using the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490), NEBNext UltraExpress RNALibrary Prep Kit for Illumina (NEB E3330L), and NEBNext Multiplex Oligos for Illumina Unique dual (NEB E6440L).