design: Total genomic DNA was isolated by the DNeasy Power Soil Pro Kit (Cat No. 47014). Isolated DNA was quantified using Nanodrop 2000 (Thermofisher Scientific, Massachusetts, USA). The integrity of DNA was evaluated on a 1% agarose (Lonza, Belgium) gel. The total genomic DNA was then subjected to shotgun metagenome sequencing on the Illumina NovaSeq platform. About 12.13 GB of raw data was obtained from the sample. Raw reads of samples were subjected to adapter trimming with FASTP, and the quality of adapter-trimmed reads was checked using FASTQC. SqueezeMeta is an automated pipeline for metagenomic and metatranscriptomic data analysis, handling multiple metagenomes simultaneously. SqueezeMeta (v1.6.3) used clean FASTQ data for analysis, including assembly of related metagenomes and genome extraction using MEGAHIT as the assembler tool, with a minimum contig length of 200 bp.