library_construction_protocol: Total RNA was purified from mid-log phase cells using Direct-zol RNA Miniprep Kit (Zymo Research) following manufacturer's instructions, including the use of ZR BashingBead Lysis Tubes to lyse open the cells in TRI Reagent. DNA was removed using DNase I (Zymo Research) and purified using RNA Clean & Concentrator (Zymo Research). Ribosomal RNAs were depleted using RiboMinus Transcriptome Isolation Kit, Yeast (Invitrogen), and libraries were made using NEBNext Ultra II Directional RNA Library Prep Kit (NEB).