library_construction_protocol: Total RNA was extracted from isolated alveolar macrophages from C. gattii-infected mice (6-10 mice/group) using a RNeasy plus mini kit (Qiagen) .  The pooled total RNA at 600 ng was used for cDNA library preparation and sequencing. The paired-end cDNA library was prepared using the NEBNext Ultra RNA library prep kit for Illumina (New England BioLabs, Ipswich, MA).