library_construction_protocol: Sample was pooled together with three cortical organoids for RNA extraction. Dissociated cells were filtered through a 40 µm cell strainer, and centrifuged at 300G for 5 mins, papain removed and resuspended in 1% FBS in PBS. Using the Chromium Single Cell 3' Reagent Kits v3.1 (10X Genomics), 4000-7000 cells were captured per lane, single-cell libraries were sequenced on a NovaSeq S2 flow cell