library_construction_protocol: Cells were trypsinized and collected and 50,000 were counted then washed in PBS. Make resuspension buffer consisting of 10nm Tris-HCl, 10 mM NaCl, and 3 mM MgCl2 in H2O. Lysis buffer consisting of resuspension buffer with 0.1% NP-40, 0.1% Tween-20, and 0.01% Digitonin was added to the cells and the mixture incubated on ice for 3 minutes. Then was wash buffer consisting of resuspension buffer with 0.1% Tween-20 was added and the cells were centrifuged at 500 x g for 10 minutes at 4C. The supernantant was discarded and the pellet was kept. The transposition reaction mix, using Nextera DNA library prep kit, was made with 1X TD Buffer, 0.1% Tween 20, 0.01% Digitonin, and Tn5 Transposase. This was added to the pellet, resuspended, and then incubated at 37C for 30 minutes on a thermomixer at 1,000 rpm. The DNA was isolated using the Qiagen MinElute Reaction Cleanup Kit and the DNA was eltured in 10uL of Elution Buffer.  For RNA, after the cells were trypsinized and collected RNA was extracted using QIAGEN RNAeasy kit. For PCR amplication, the purified transposed DNA was combined with Ad1_noMX primer, Ad2.*indexing primer, and NEBNext High-Fidelity 2X PCR Master Mix. The samples were amplified with the following program: 72C for 5 mins, 98 C the 30 seconds, then for five cycles to 98C for 10 seconds, 63 C for 30 seconds, and 72 C for 1 minute. Then, the PCR was continued after testing the appropriate number of cycles, for 98C for 30 seconds then 8 cycles of 98C for 10 seconds, 63C for 30 seconds, and 72C for 1 minute. The library was then purified using Zymo DNA clean and concentrator 5.