library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Zymo Research, kit reference R2050) following the manufacturer's protocol with minor modifications. Briefly, cells were lysed in 1 mL of TRIzol reagent (or 500 µL for 12-well plates), followed by immediate vortexing and incubation at room temperature for 10 minutes or freezing at −80°C. Chloroform (200 µL) was added to each sample, which were then vortexed for 10 seconds and incubated for 2 minutes at room temperature before centrifugation at 16,000 × g for 15 minutes at 4°C. The aqueous phase was carefully collected, avoiding the interphase and organic phase, and RNA was precipitated by adding 300 µL of isopropanol, mixing thoroughly by pipetting. The mixture was then loaded onto Zymo-Spin IICR columns and centrifuged at 16,000 × g for 30 seconds at room temperature. Columns were washed with RNA Wash Buffer and subjected to on-column DNase I treatment (5 µL DNase I in 75 µL DNA Digestion Buffer) for 15 minutes at room temperature to remove residual genomic DNA. Following DNase treatment, columns were washed twice with Direct-zol RNA PreWash buffer and once with RNA Wash Buffer, with centrifugations at 16,000 × g for 30 seconds to 1 minute. The RNA was eluted in 60 µL of DNase/RNase-free water after a final centrifugation at 16,000 × g for 30 seconds. 2ug of RNA was sent to Genewiz who carried out library prep and subsequent sequencing.