library_construction_protocol: Total CD66b+ neutrophils from the LDN and NDN fractions of peripheral blood from NSCLC patients and G-CSF-treated donors, and the NDN fraction of peripheral blood from healthy donors (HDs) as controls, were isolated by magnetic bead selection with fluorescence-conjugated anti-CD66b mAbs and processed with BD Rhapsody Single-Cell Analysis System for scRNA-seq. cDNA Libraries were prepared using the BD Rhapsody Whole Transcriptome Analysis (WTA) Amplification kit and BD Single-Cell Multiplexing kit (BD Biosciences) following the manufacturer's protocol