library_construction_protocol: RNA was extracted from ~ 5 million cells using the Qiagen RNeasy Kit for RNA (74104) purification following the manufacturer's instructions. mRNA was purified using the NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490S) using 1 µg of input RNA. Library preparation for sequencing was done using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760S).