library_construction_protocol: Naivet T cells were activated by treatment of 1.5 ug/mL anti-mouse-CD3 (Bio X Cell) and soluble 1.5 ug/mL anti-mouse-CD28 (Bio X Cell). IL-12 (10 ng/mL) in the 25mM glucose medium to induce Th1 cell until day 3. Cells were then harvested. RNA was harvested using RNeasy mini kit (Qiagen). 2 ug total RNAs were used for stranded RNA sequencing library. RNA library for RNA-seq was prepared using KCTM Stranded mRNA Library Prep Kit for Illumina (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction.