library_construction_protocol: Livers were perfused with PBS and harvested in cold PBS. A 50mg portion was collected in disassociation buffer (0.15% Collagenase P (Roche) in HBSS) and incubated at 37˚c until complete disassociation. Cells were treated with ACK for the removal of red blood cells. Cells were washed and strained and kept in full growth medium (DMEM supplemented with 10% fetal calf serum (FCS) (Thermo Scientific, Waltham, MA, USA)). Dead cells were removed using Dead Cell Removal Kit (Miltenyi Biotec, Auburn, USA) according to the manufacturer protocol. Cells were counted manually, and dead cell percentages were calculated using trypan blue. Only samples with less than 10% dead cells were used for sequencing. The cells were subjected to the Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (10× Genomics, CA, USA) and libraries were prepared for sequencing following manufacturer company instructions. Sequencing was done using NovaSeq 6000 system machine (Illumina) with the following sequencing conditions: 28 bp (Read1) and 90 bp (Read2) using the NovaSeq 6000 SP Reagent Kit v1.5 (100 cycles) (Illumina).