design: Essential gene screening identifies the Bromodomain-Containing Protein BRPF1 as a new therapeutic target for endocrine therapy-resistant breast cancer
library_construction_protocol: MCF-7 were purchased from the American Type Culture Collection (ATCC). Cell lines were cultured according to manufacturer's protocol and as indicated,  MCF-7 cell lines treated with 10 µM GSK6853 and its vehicle for 6 days. Biological replicates were carried out in duplicate and ~100,000 nuclei were loaded per reaction  collecting directly into the cold ATAC-seq lysis buffer (Active Motif cat. 53150). Library preparation was carried out per manufacturer's instructions (Active Motif cat. 53150). Indexed library concentrations were measured using Quant-IT RNA Assay Kit-High Sensitivity and a Qubit Fluorometer (Life Technologies, Monza, Italy) and integrity assessed with the Agilent 4200 Tapestation System (Agilent Technologies, Milan, Italy)