design: MiSeq rRNA Amplicon Sequencing protocol (Illumina, San Diego, CA) with some modifications. PCR was performed in a final volume of 25 L, which was set up with the following quantities: 5 L of genomic DNA (10 ng/L in H2O), 1x PCRBIO HiFi Buffer (PCR BIOSYSTEMS, USA) composed by 1 mM dNTPs and 3 mM MgCl2, 0.5 units of PCRBIO HiFi Polymerase (PCR BIOSYSTEMS, USA) and 0.2 M of each primer. Cycling conditions followed an initial denaturation at 95 C for 3 min, 25 cycles of 95 C (30 s), 55 C (30 s), 72 C (30 s), final extension at 72 C for 5 min, hold at 4 C.