library_construction_protocol: Total RNA was extracted from lung and kidney tissues using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA quantity and quality were assessed using the Agilent 2100 Bioanalyzer and RNA 6000 Nano LabChip Kit. Only RNA samples with RIN > 7.0 were used for library construction. mRNA was purified from total RNA using oligo(dT) magnetic beads and fragmented into short fragments. First-strand cDNA was synthesized using reverse transcriptase, followed by second-strand cDNA synthesis. After end repair and A-tailing, sequencing adapters were ligated to the fragments. The ligated products were amplified by PCR and purified to construct sequencing libraries with an average insert size of approximately 300 bp. Paired-end sequencing (2 × 150 bp) was performed on an Illumina NovaSeq 6000 platform.