library_construction_protocol: Total RNA was extracted from sgCtrl or NF2-KO PM cells via the QIAwave RNA Mini Kit.An input of 1 μg of RNA per sample was employed for the RNA sample preparations. In alignment with the manufacturer's guidelines, sequencing libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA), with index codes incorporated to facilitate the assignment of sequences to each sample.