design: Total RNA was isolated from freshly collected tissue specimens using the MGIEasy RNA Prep Kit (MGI, #MG1000006386), following the manufacturers instructions to ensure high-quality RNA suitable for downstream library construction. To selectively enrich messenger RNAs and eliminate ribosomal RNA, 200 ng of total RNA from each sample was subjected to poly(A)+ selection using the Dynabeads mRNA Purification Kit (Thermo Fisher Scientific, #01152851), which captures polyadenylated transcripts via oligo(dT)-coated magnetic beads. The resulting mRNA was then used for directional library preparation with the MGIEasy RNA Directional Library Prep Set (MGI, #MG1000006386), enabling strand-specific transcriptome profiling.Library fragment size distribution was assessed on an Agilent 4150 TapeStation system (Agilent Technologies, #G2992A) using the High Sensitivity D1000 ScreenTape Assay (Agilent, #5067-5582) to confirm appropriate insert sizes and the absence of primer-dimer artifacts. Final library concentrations were determined using the Qubit 2.0 Fluorometer (Thermo Fisher Scientific, #Q32866) in combination with the Qubit RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, #Q32854). Paired-end RNA sequencing (2  150 bp) was conducted on the DNBSEQ-T7 platform (MGI).