library_construction_protocol: QIAGEN RNAeasy Mini Kit NEBNext Ultraexpress RNA Library Prep Kit for Illumina (E3330S - New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis (E7490 -  New England BioLabs Inc.) with the addition of barcode sequences