design: All muscle samples were processed and snRNA-seq data generated by GENINUS (Seoul, Republic of Korea). After the frozen tissue was homogenized and nuclei were counted, the nuclei were isolated using flow cytometry. Single-cell capture, barcoding and library preparation were performed following the 10x Genomics Single cell Chromium 3 protocols (V3: CG000183). cDNA library quality was determined using an Agilent Bioanalyzer. Paired-end 200 bp reads were generated on an Illumina NovaSeq6000.