design: Genomic DNA was purified and used as input for PCR 1 using amplicon-specific primers. After confirmation of PCR 1 product, PCR clean-up was performed using magnetic beads and purified PCR products were used as input for index ligation. After confirmation of index ligation on agarose gels, samples were roughly pooled at equimolar ratios and loaded on a gel for purification. Last, a 4nM library was prepared using a Qubit fluorometer.