library_construction_protocol: mESCs nuclei were isolated by incubation with Complete Nuclei Isolation Buffer according to the manufacturer's instructions. ATAC-seq was performed by using the ATAC-Seq Kit (Active Motif) according to the manufacturer's instructions. CUT&Tag was performed by using the CUT&Tag-IT® Core Assay Kit (Active Motif) following the manufacturer's instructions. CUT&RUN was performed by using the ChIC/CUT&RUN Assay Kit (Active Motif) following the manufacturer's instructions.