design: Engineered DNA polymerase and 4 fluorescent labels are added   dNTPs , which incorporate only a single base per cycle ; Scan the surface of the plate with a laser and read the first round of each template sequence                                                                                                                                           The type of nucleotide that should be polymerized ; The "fluorophore" and "stopper" are chemically cleaved to restore the 3' end stickiness and continue to polymerize                                                                                                                         Combine the second nucleotide: The results of the fluorescent signal collected in each round are counted to obtain the sequence of the template DNA fragment . about  Nanopore sequencing, after the library is completed, a certain concentration and volume of DNA library is added to the flow cell, and the flow cell is added  Transfer to an Oxford Nanopore PromethION sequencer for real-time single-molecule sequencing