design: Overnight cultured S. aureus RN4220 was inoculated into fresh BHI broth at a dilution of 1:100 and cultured for 2 h at 37C with or without 1/2 MIC of OXA treatment. Bacterial cells were harvested and its total RNA was isolated using the Trizol Reagent (Invitrogen Life Technologies). A Zymo-Seq RiboFree Total RNA Library Kit was used to remove rRNA from the total RNA sample. cDNA libraries were built using the Next Ultra RNA Library Prep Kit (New England Biolabs, USA). The RNA sequencing (RNA-seq) was performed on NovaSeq 6000 platform (Illumina). The reference genome and gene annotation files were downloaded from genome website (https://www.ncbi.nlm.nih.gov/datasets/genome/GCF_000212435.1/).