library_construction_protocol: Mouse leukemia cells isolated from leukemic mice, MP cells (Lin-, c-Kit+), and c-Kit-enriched HSPCs were lysed with Trizol (Ambion 15596018) for total RNA extraction. mRNA from 2 µg of total RNA was enriched with VAHTS mRNA capture beads and fragmented to 200-500 nt lengths using 2× ProtoScript II buffer. Strand-specific RNA-seq libraries were prepared and sequenced with 150 bp paired-end reads on the NovaSeq 6000 platform.