library_construction_protocol: total RNA was extracted using MJzol Animal RNA Isolation Kit (Majorivd) and according to the standard operating procedures provided by the manufacturer. Purification was performed using the RNAClean XP Kit (Beckman Coulter) and RNase-Free DNase Set (QIAGEN). RNA integrity was detected by Agilent 2100 Bioanalyzer/Agilent 4200 TapeStation (Agilent technologies). The total amount and purity of RNA were determined by Qubit 2.0 fluorescence quantifier (Thermo Fisher Scientific) and NanoDrop ND-2000 spectrophotometer (Thermo Fisher Scientific).