design: Total RNA was extracted from Streptomyces strains M4  using the TRIzol reagent (Invitrogen) according to the manufacturer's protocol. The quality and integrity of the RNA samples were evaluated using a NanoDrop spectrophotometer and agarose gel electrophoresis. Ribosomal RNA was removed using the Ribo-Zero rRNA Removal Kit (Illumina) to enrich for mRNA. Sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), following the recommended protocol. In brief, the enriched mRNA was fragmented and reverse transcribed into cDNA. The cDNA was then end-repaired, A-tailed, and ligated to Illumina sequencing adapters. The libraries were amplified by PCR and purified with AMPure XP beads (Beckman Coulter). Library quality was assessed using an Agilent 2100 Bioanalyzer, and the final libraries were quantified using a Qubit Fluorometer (Thermo Fisher Scientific). Paired-end sequencing (150 bp) was performed on an Illumina NovaSeq 6000 platform.