library_construction_protocol: Genomic DNA was extracted from whole blood using the DNeasy Blood and Tissue Kit (QIAGEN), according to the manufacturer's protocol. The bisulfite conversion process was performed using the Zymo Research EZ DNA Methylation Gold Kit. For library preparation, the ABclonal Scale Methyl-DNA Lib Prep Kit was utilized. The conversion process, library preparations, and sequencing were performed externally at Novogene.