library_construction_protocol: Total RNA of each sample was extracted using TRIzol Reagent/RNeasy Mini Kit (Qiagen), and was qualified by Agilent 2100/2200 Bioanalyzer (Agilent Technologies) and NanoDrop (Thermo Fisher). 1 μg of total RNA was used for following library preparation. Libraries with different indices were multiplexed and loaded on an Illumina HiSeq/Novaseq instrument (Illumina) according to instructions of the manufacturer.