library_construction_protocol: DCs sorted from pLNs of C57BL/6 mice. The cellular samples were loaded in the lanes according to the 10X Genomics Chromium Next GEM Single Cell 3'v4 (CG000732 Rev B) User Guide with a single capture lane per sample. 3' v4 gene expression profiling and feature barcode libraries (Total Seq-B)  were performed as described in the vendor provided user guide.