library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit (Qiagen, Cat# 74104) according to the manufacturer's instructions, including on-column DNase treatment (Qiagen RNase-Free DNase Set, Cat# 79254) where applicable. RNA integrity and concentration were assessed prior to library preparation RNA libraries for RNA-seq were prepared using Qiagen RNeasy Mini Kit (74104) following manufacturers' protocols