design: 100 ng of total RNA were used to generate barcoded RNA-Seq libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs Inc). Libraries were sequenced on a NextSeq 2000 (Illumina) to generate 100 bases single-end reads. FastQ files for each sample were obtained using bcl2fastq v2.20.0.422 software (Illumina).