design: C. amycolatum ICIS 60 was isolated from the vaginal contents of healthy women and is part of the Collection of Microorganisms of the Institute for Cellular and Intracellular Symbiosis UrB RAS (Orenburg, Russia). The strain was grown in LuriaBertani (LB) Miller broth (Becton Dickinson, USA) at 37 C for 24 h and sub-cultured once in the same medium at 37 C for 24 h before use. Genomic DNA was extracted using a Quick DNA Fungal/Bacterial Kit (Zymo Research, USA). The quality of the extracted DNA was assessed according to the A260/280 ratio using a Nanodrop 8000 (Thermo Fisher Scientific, USA), and electrophoresis was performed in a 1% agarose gel. DNA concentration was quantified using a Qubit 4 Fluorometer and a dsDNA High Sensitivity Assay Kit (Life Technologies, USA). The DNA library for whole-genome sequencing was prepared using a NEBNext Ultra II FS DNA Library Prep Kit for Illumina (New England BioLabs, USA). The library was validated using capillary electrophoresis on a QIAxcel Advanced System (Qiagen, Germany) using the QIAxcel DNA High Resolution Kit and normalized using qPCR on the CFX Connect Real-Time PCR System (Bio Rad, USA). Paired-end sequencing (2300 bp) was carried out on a MiSeq platform (Illumina, USA) using Reagent Kit v.3 (Illumina, USA) in the  Persistence of microorganisms Science Resource Center of the Institute for Cellular and Intracellular Symbiosis, UrB RAS.