design: Following the successful completion of quality control, genomic DNA is fragmented with the aid of g-TUBEs (Covaris, USA) in accordance with the desired fragment size, with the aim of constructing the library. The subsequent steps involve magnetic bead enrichment and purification of the target fragment DNA. The fragmented DNA then undergoes a process of damage repair and end repair. Stem-loop sequencing adapters are then ligated to both ends of the DNA fragments, and failed ligation fragments are removed using an exonuclease. The target fragments were then subjected to screening using a gel cutting instrument (BluePippin, SageScience, USA), and the purified fragments were used as the library.