library_construction_protocol: Protein associated RNAs were released by proteinase K and were extracted by phenol/chloroform. Following sample qualification, 1–10 ng of total RNA was utilized for library construction using the Clontech SMARTer Stranded Total RNA-Seq Kit V2 (Pico Input). Briefly, RNA was fragmented and combined with a 5'-end switching template to initiate reverse transcription for first-strand cDNA synthesis. Subsequently, index-containing adapters were ligated during the first-round PCR amplification. The ligation products were purified, and the optimal number of amplification cycles was selected based on the initial RNA quantity for library enrichment. Finally, the amplified libraries were purified using magnetic beads to generate strand-specific RNA-Seq libraries ready for sequencing.