design: Complete genome of the sample was amplified using a single multisegment reverse transcription-PCR (M-RTPCR) following the method by Zhou et al. The reaction utilized 5 l of extracted RNA as input. PCR product was visualized on 1% agarose gels and purified using AMPure magnetic beads (Beckman Coulter Genomics) and the DNA Clean & Concentrator kit (Zymo Research).