library_construction_protocol: The sequencing library was constructed by capturing cells/nuclei with ConA magnetic beads, permeabilizing them with digitonin, and performing antibody-guided in situ tagmentation using pA-Tn5 transposase to simultaneously fragment target-proximal DNA and add sequencing adapters. The library was then amplified by PCR and purified with AMPure beads. CUT&Tag libraries were prepared using the CUT&Tag_Novogene Kit following the method described by Kaya-Okur et al. Cells were immobilized on concanavalin A-coated magnetic beads, and permeabilized with Digitonin. The pA-Tn5 transposome was guided by antibodies to tagment DNA near the target protein. Adapters were simultaneously added at both ends. Libraries were PCR-amplified and purified with AMPure XP beads. Library quality was assessed using the Agilent Bioanalyzer 2100 system.