design: SpRNAseq was performed using the Curio Seeker 3x3 Spatial Mapping Kit (Curio Bioscience) and spinal cords were prepared according to the manufacturers instructions. Briefly, POVPC was injected into the spinal cords of two 52wk female C57Bl/6J mice as stated above. After 42 days, the spinal cord was collected and snap frozen with liquid nitrogen and embedded into FSC 22 Frozen Section Media (Leica). Using a cryostat (ThermoFisher Scientific), spinal cord tissue was cut coronally into 10 m sections, collected on to the 3x3 Curio Seeker tile. Tissue RNA hybridization, reverse transcription, tissue clearing, bead resuspension, 2nd strand synthesis, cDNA amplification, cleanup and quality control, as well as library construction were performed according to manufacturers instructions. Sequencing was completed using Illumina NextSeq 2000 with P4 XLEAP (100 cycles).