design: RNA-seqlibraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB,Cat. No. E7770) following the manufacturers protocols. Briefly, mRNA was fragmented to anaverage size of 200 nt by incubating at 94C for 15 min in the first strand buffer. cDNA was thensynthesized using random primers and ProtoScript II Reverse Transcriptase followed by secondstrand synthesis using NEB Second Strand Synthesis Enzyme Mix. Resulting DNA fragmentswere end-repaired, dA tailed, and ligated to NEBNext hairpin adaptors (NEB, Cat. No. E7335).Following ligation, adaptors were converted to the Y shape by treating with USER enzyme,and DNA fragments were size selected using Agencourt AMPure XP beads (Beckman Coulter#A63880) to generate fragment sizes between 250-350 bp. Adaptor-ligated DNA was PCRamplified followed by AMPure XP bead clean up. Libraries were quantified using a QubitdsDNA HS Kit (ThermoFisher Scientific, Cat. No. Q32854), and the size distribution wasconfirmed using a High Sensitivity DNA Kit for Bioanalyzer (Agilent Technologies, Cat. No.5067- 4626).