library_construction_protocol: After 48h of ASO transfection, cells were lysed in LRT plus lysis buffer (Qiagen), and RNA isolation was performed using an RNeasy mini kit (Qiagen) following the manufacturer's instructions. From each sample, 500ng of RNA was used for the library preparation using the kit (QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD with UDI, Lexogen), pooled together with an equal concentration, and then sequenced on a NovaSeq X+, paired-end, 150 bp length, with ~30 M reads for each sample.