library_construction_protocol: Then genomic DNA was extract by Wizard Genomic DNA purification Kit(Promega, A1120). And DNA fragmented by sonication to a mean size of approximately 250bp (Covaris, M220) the ligated DNA was bisulfite-converted using the MethylCode™ Bisulfite Conversion Kit (Invitrogen, MECOV50), and the construction of library follow the protocol of  EpiArt® DNA Methylation Library Kit for Illumina V3 (Vazyme, NE103)