library_construction_protocol: RNA was extracted from each sample using TRIzol LS, according to the manufacturer's instructions. The total RNA concentration was measured with the Qubit RNA High-sensitivity assay (Invitrogen) on a Qubit 4 Fluorometer (Invitrogen). The quality of the RNA was validated with the Agilent High Sensitivity RNA ScreenTape assay at an Agilent TapeStation 4000 instrument, with all samples having a quality score RIN ≥ 9.1. Library preparation was performed with the Lexogen QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices according to the manufacturer's instructions. Each replicate was processed individually to avoid batch effect. 330 ng total RNA in 5 μL volume was used as input. The amplified libraries were quantified and validated with the Qubit dsDNA High-sensitivity and Agilent D1000 high sensitivity ScreenTape assays, pooled in an equimolar ratio, and deep sequenced on an Illumina NextSeq 2000 instrument, using a single-end 100 nt reverse complement workflow. Sequencing was performed at the Barts and The London Genome Centre. Library preparation was performed with the Lexogen QuantSeq 3' mRNA-Seq V2 Library Prep Kit FWD with Unique Dual Indices according to the manufacturer's instructions. Each replicate was processed individually. 330 ng total RNA in 5 μL volume was used as input. The amplified libraries were quantified and validated with the Qubit dsDNA High-sensitivity and Agilent D1000 high sensitivity ScreenTape assays, pooled in an equimolar ratio, and deep sequenced on an Illumina NextSeq 2000 instrument, using a single-end 100 nt reverse complement workflow. Sequencing was performed at the Barts and The London Genome Centre, Queen Mary University of London.