library_construction_protocol: Post mortem tissue collection: Human post mortem brain tissue samples were obtained from the Human Brain Tissue Bank (Semmelweis University, Budapest, Hungary). Tissue samples were collected from 31 individuals who had not been diagnosed with any psychiatric disorder and neurodegenerative disorders. The brains were removed with a PMI of 2 – 12 hours, drop frozen using dry ice, and stored at −80 °C until further dissection. Samples were punched out from the lateral surface of the prefrontal cortices of the diseased subjects using specialized microdissection needles with 8- and 15-mm internal diameters. While most of the samples were dissected from the ventrolateral prefrontal cortex, some originated from the dorsolateral prefrontal cortex and from the middle frontal gyrus (the dorsal part of which belongs to the dorsolateral prefrontal cortex and its ventral part to the ventrolateral prefrontal cortex). The dissected cortical tissue pellets included both gray and white matter portions within the gyrus. Samples were collected in 1.5 mL Eppendorf tubes and stored at −80 °C until further use. Throughout the microdissection procedure, the tissue samples were kept frozen.  Single-nuclei Isolation: The nuclei isolation from the frozen post mortem brain tissue was performed as described previously in Soderstan et. al. 2018 and Jonsson et al 2021. Briefly, the tissue was thawed and dissociated in ice-cold lysis buffer [0.32 M sucrose, 5 mM CaCl2, 3 mM MgAc, 0.1 mM EDTA, 10 mM tris-HCl (pH 8.0), and 1 mM dithiothreitol] using a 1 ml tissue douncer (Wheaton). The homogenate was carefully layered on top of a sucrose cushion [1.8 M sucrose, 3 mM MgAc, 10 mM tris-HCl (pH 8.0), and 1 mM dithiothreitol] before centrifugation at 30,000 g for 2 hours and 15 minutes. After supernatant removal, the pelleted nuclei were softened for 10 minutes in 50 μl of nuclear storage buffer [15% sucrose, 10 mM tris-HCl (pH 7.2), 70 mM KCl, and 2 mM MgCl2] before being resuspended in 300 μl of dilution buffer [10 mM tris-HCl (pH 7.2), 70 mM KCl, and 2 mM MgCl2] and filtered through a cell strainer (70 μm). Nuclei were stained with Draq7 and run through FACS (FACS Aria, BD Biosciences) at 4°C at a low flow rate using a 100 μm nozzle (reanalysis showed >99% purity). 8,500 nuclei were sorted and used for downstream applications (snRNA Sequencing). Detailed protocol can be found at doi: dx.doi.org/10.17504/protocols.io.5jyl8j678g2w/v1. Nuclei intended for single-nucleus RNA-sequencing (8,500 nuclei per sample) were directly loaded onto the Chromium Next GEM Chip G or Chromium Next GEM Chip K Single Cell Kit along with the reverse transcription master mix following the manufacturer's protocol for the Chromium Next GEM single cell 3′ kit (PN-1000268, 10x Genomics), to generate single-cell gel beads in emulsion. cDNA amplification was done as per the guidelines from 10x Genomics using 13 cycles of amplification. Sequencing libraries were generated with unique dual indices (TT set A) and pooled for sequencing on a Novaseq6000 using a 100-cycle kit and 28-10-10-90 reads.