library_construction_protocol: Pooled 8:2 CD45+:CD45- cells were resuspended in Cell Staining Buffer (BioLegend) and stained with a pool of 137 TotalSeq-C antibodies (Table) according to the manufacturer's protocol before loading onto the Chromium Controller (10X Genomics) for GEM generation. The cDNA libraries were generated using all or a subset of Chromium Next GEM Single Cell 5′ Library Kit for gene expression (GEX), Chromium Single Cell V(D)J Enrichment kit (10X Genomics) for T cell receptor (TCR), and Chromium Single Cell 5′ Feature Barcode Library kit for antibody derived tag (ADT) according to the manufacturer's instructions