library_construction_protocol: Frozen worms were homogenized in an MM400 Mixer Mill homogenizer (Retsch) for 2 rounds of 1 min at a frequency of 30-1 sec. An estimated equal amount of worm powder per genotype was added to a 15 mL conical and 1.5 mL IP buffer (10% glycerol, 10 mM EDTA,  30 mM Hepes (pH 7.4), 100 mM potassium acetate, 2 mM DTT, 0.1% NP40) was added. Worm powder was resuspended in the buffer by pipetting and vortexing. Worm lysis was transferred to a 2 mL LoBind microcentrifuge tube (Eppendorf) and spun at 18000 g for 5 min at 4°C. The lipid layer was aspirated off. 50 mL of the supernatant was reserved as the 'input' sample and stored in 1 mL TriReagent (ThermoFisher) at -80°C. The rest of the worm lysis was mixed with 50 mL Dynabeads conjugated with anti-FLAG antibody (Sigma-Aldrich, F1804) and nutated at 4°C for two hours. Immunoprecipitated material was washed 3x in cold IP buffer. Immunoprecipitated material (on Dynabeads) were stored in 1 mL TriReagent (ThermoFisher) at -80°C. Worms were collected in TriReagent (ThermoFisher) and subjected to three freeze-thaw cycles. Phase separation was achieved via 1-bromo-3-chloropropane (BCP) and the aqueous phase was precipitated in isopropanol at -80°C for 2 hrs. To pellet RNA, samples were centrifuged at 21,000 x g for 30 min at 4°C. The pellet was washed three times in 75% ethanol then resuspended in water. RNA-seq libraries were generated using the KAPA stranded mRNA-seq kit (Roche) according to the manufacturer's instructions. Small RNA-seq libraries were made using the NEBNext Multiplex Small RNA Library Prep Set (NEB) according to the manufacturer's instructions. All RNA-seq libraries were sequenced on a NovaSeq 6000 Sequencer (Illumina).