library_construction_protocol: Intestinal crypts were disaggregated by pre-cold PBS with 30 mM EDTA. Total RNAs were extracted by TriZol (Invitrogen) according to manufacturers' recommendations. 1 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using TsruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA) following manufacturer's protocols.