library_construction_protocol: Total RNA was extracted using RNeasy Mini Kit following the manufacturer's protocol. RNA integrity and purity were assessed using a Qubit® Fluorometer (Life Technologies) and a Fragment Analyzer (Agilent). Samples with a 260/280 nm ratio between 1.8 and 2.1 and a 28S/18S ratio between 1.5 and 2 were considered suitable for sequencing. RNA samples (100–1000 ng) underwent poly(A) enrichment and were reverse transcribed into double-stranded cDNA using the TruSeq Stranded mRNA Kit (Illumina).   cDNA libraries were enzymatically fragmented, end-repaired, adenylated, and ligated with TruSeq adapters containing unique dual indices (UDI). Adapter-ligated fragments were enriched by PCR amplification, and final libraries were assessed for quality and quantity using a Qubit® Fluorometer and Fragment Analyzer. The final product had an average fragment size of ~260 bp and was normalized to 10 nM in Tris-Cl (10 mM, pH 8.5, 0.1% Tween-20) before sequencing. Sequencing was performed on an Illumina NovaSeqX platform using a paired-end 2 × 150 bp strategy. Raw reads were pre-processed using fastp (S. Chen et al. 2018) with the following parameters: --trim_front1 1 --cut_tail 20 --trim_poly_x --poly_x_min_len 10 --length_required 18.  Transcript quantification was performed with kallisto using the GRCh38.p13 reference transcriptome (Annotation Release 42, downloaded on 2023-01-30, doi:10.1038/nbt.3519). Differential gene expression analysis was conducted using EdgeR (Robinson, McCarthy, and Smyth 2010). All computational analyses, including Gene Set Enrichment Analysis (GSEA) and Over-Representation Analysis (ORA), were executed via the SUSHI platform at the Functional Genomics Center Zurich (Hatakeyama et al. 2016). cDNA libraries were enzymatically fragmented, end-repaired, adenylated, and ligated with TruSeq adapters containing unique dual indices (UDI). Adapter-ligated fragments were enriched by PCR amplification, and final libraries were assessed for quality and quantity using a Qubit® Fluorometer and Fragment Analyzer. The final product had an average fragment size of ~260 bp and was normalized to 10 nM in Tris-Cl (10 mM, pH 8.5, 0.1% Tween-20) before sequencing. Sequencing was performed on an Illumina NovaSeqX platform using a paired-end 2 × 150 bp strategy. Raw reads were pre-processed using fastp (S. Chen et al. 2018) with the following parameters: --trim_front1 1 --cut_tail 20 --trim_poly_x --poly_x_min_len 10 --length_required 18.