library_construction_protocol: Cells were broken using freezer mill (Spex SamplePrep,6875D) for 5 cycles, 2 min, 15cps. After verification of breakage under the microscope, cells were resuspended in 900μL of XLB-3 (supplemented with protease inhibitors, A32955, Pierce) and cross-linked by addition of DSG at 3mM final (Disuccinimidyl glutarate, A35392, ThermoFisher) for 30min at 30C. DSG was quenched by addition of 100μL of 1M Tris HCl, pH 8 for 1min. SQK-LSK114