design: Transcriptomic analysis was performed on transgenic rice overexpressing the Kentucky bluegrass (Poa pratensis L.) gene PpZAT12 and the wild type (Zhonghua 11). Two-week-old rice seedlings grown in Kimura B nutrient solution were subjected to osmotic stress for 24 hours using a solution containing 23% PEG-6000, with seedlings in normal nutrient solution serving as the control. Total RNA was isolated from leaf samples using TRIzol reagent. Sequencing libraries were constructed by enriching polyadenylated mRNA using Oligo(dT) beads, and were sequenced on an Illumina NovaSeq 6000 platform to generate 150 bp paired-end reads.