design: scRNA-seq of motor neuron and microglia co-cultures derived from C9ORF72 HRE iPSCs (ALS patient), C9ORF72 KO iPSCs and control iPSCs in unstimulated and LPS stimulated conditions
library_construction_protocol: Unstimulated or LPS-primed co-cultures were dissociated using Accutase™ and resuspended in DMEM/F-12 supplemented with 0.04% BSA and 10 µM Y-27632 (72304, StemCell Technologies). Cells were further dissociated by gentle pipetting and filtered through a 35 µm cell strainer (100-0087, StemCell Technologies) before suspension in PBS(-/-) supplemented with 0.04% BSA and 10 µM Y-27632. For each sample, approximately 30,000 cells were pooled from three independent differentiations. Performed according to HT v3.1 Performed according to HT v3.1