library_construction_protocol: Reverse crosslinking buffer was applied to tissue section (50 mM of Tris-HCl, 1 mM EDTA, 1% (wt/vol) of SDS, 200 mM NaCl, 0.4 mg-mL Proteinase K, nuclease-free H2O) was added to each well, then incubated at 58˚C for two hours. Each lysate was further incubated overnight at 65˚C with agitation. Nextera XT Library Prep Kit was used and the Tagment Mix (1ng of purified cDNA in RNase-free water to a volume of 5 µL, 10 µL of Tagment DNA buffer, 5 µL of Amplicon Tagment Mix) was incubated at 55˚C for 5 minutes. NT Buffer (5 µL) was added then incubated at room temperature for 5 minutes. The final PCR mix was added (15 µL PCR master mix, 1 µL of 10 µM Ad1.X primer, 1 µL of 10 µM N70x primer, 8 µL of RNase-free water) and thermocycling was completed (Stage 1 – 1 cycle: 95˚C for 30 seconds, Stage 2 – 12 cycles: 95˚C for 10 seconds – 55˚C for 30 seconds – 72˚C for 30 seconds, Stage 3 – 1 cycle: 72˚C for 4 minute – 4˚C hold). Next Generation Sequencing was completed at NGI (National Genomics Infrastructure) using IllµMina NovaSeq X Plus 25B in paired-end 150bp mode with a total of 6 million reads for each sample and a 15% PhiX sequencing step.