library_construction_protocol: Samples were then enzymatically and mechanically digested using the mouse tumor dissociation kit (Miltenyi Biotec) and the gentleMACs Octo Dissociator (Miltenyi Biotec). Following dissociation, samples were passed through a 100-micron cell strainer and were washed with RMPI containing 2% fetal bovine serum. Samples were then resuspended in buffer containing 0.05% BSA in PBS. Single cell suspensions quality, number and viability were assessed with a dual fluorescence cell counter LUNA-FX7TM (Logos Biosystems). 10,000 cells were targeted from each sample cell suspension. The cells were washed twice with PBS+0.04% BSA and resuspended in about 500 cells µl-1.10X Genomics' Chromium instrument and Dual index Single-Cell 3′ Reagent kit (V3.1) were used to prepare the individually barcoded single-cell RNA-Seq libraries following the manufacturer's protocol.  Quality of the libraries were assessed by the TapeStation-4200 traces (Agilent BioAnalyzer High Sensitivity Kit) and quantitated by the Qubit system. Sequencing was done on the Illumina NextSeq-2000 machine using the P3 sequencing Kit (Illumina).