design: All samples were sequenced using Illumina (NOVASEQ 6000), with an average sequencing depth obtained of 6GB/sample and a read length of both forward and backwards of 150 bp, the total number of reads (both forward and backward), the trimming size of the read, and the total size of the data generated after the sequencing per sample. The raw reads were analysed using an in-house WES pipeline, which we benchmarked earlier.Briefly, with the hg38 reference build, the pre-processing of raw data was performed with a sequence quality check using FastQC. Bowtie2 was used to  align with hg38  reference build, and the obtained SAM files were converted to Bamtools, sorted and mpileup before calling variants using VarScan. The final list of VCF files was re-processed through bcftools and vcftools before being analysed through different tools and databases, such as SNPnexus, NCBI-dbSNP, and Batch Entrez.