design: The cell pellets were subjected to lysis and extraction using the Trizol method. The nucleic acid was subjected to enzymatic fragmentation to produce dsDNA fragments followed by end repair and A-tailing to produce end-repaired ,5'-phosphorylated, 3'-dA-tailed dsDNA fragments. This was followed by adapter ligation where dsDNA adapters with 3'-dTMP overhangs are ligated to the 3'-dA-tailed molecules. The libraries were normalized, pooled and sequenced using NovaSeq X plus.