library_construction_protocol: After centrifugation, the supernatant was removed, and the bacterial cells were processed using the AccuPrep® Bacterial RNA Extraction Kit (Bioneer, K-3143), following the manufacturer's protocol. RNA libraries was prepared using Illumina TruSeq Stranded mRNA Sample Prep Kit (Illumina, Inc., San Diego, CA, USA, #20020595) following manufacturer's protocols