design: Genomic DNA was extracted from Atlantic sturgeon blood with DNeasy Blood and Tissue kit and the resulting genomic DNA was sheared with a nebulizer. paired-end libraries were prepared from 5 micrograms of sheared DNA using the Pair-End Sequencing Sample Prep kit . The resulting library was run on gel and the 400bp band was cut. All libraries were sequenced using an Illumina HiSeq2500 instrument with a read length of 151nt.