library_construction_protocol: Monocytes were isolated from 30mL of blood from a single healthy and OPC-diagnosed individuals. Briefly, leukocytes were enriched by Ficoll separation and isolated by negative selection (Miltenyi). Conditioned media was generated by incubating sub confluent SCC-25 or SCC-154 cell lines in E-media supplemented with 2% FCII. Monocytes were incubated for 18 hours in E2 media (control) or in E2 media diluted 1:2 with SCC-25, SCC-154 CM or E-media containing recombinant human IL1α to a final concentration of 200 pg/mL. Cells were incubated for 18 hours. Cells from each stimulation condition (4) were multiplexed for each individual and libraries prepared accoding to manufacturer's protocols (10X Genomics)