design: DNA was extracted from 5uM eDNA filter using a Modified QIAGEN DNeasy Blood and Tissue extraction protocol (Milin-Garca, 2022); libraries were created for amplicon sequencing following the protocols described in the 16S Metagenomic Sequencing Library Preparation Guide (Illumina), with the first-round of PCR following the methods described by Miya et al. (2015)