design: As plant material, the restricted branching yellow lupin (Lupinus luteus L.) cv. Taper was used. Seeds were provided by the Poznaska Hodowla Rolin Ltd (Wiatrowo Branch, Poland) and sown into 10 dm3 pots containing fifth-class soil (Polish bonitation classification of soils). The anthers being in post-dehiscence phase (LAD3, late anther development phase 3, acc. to Marciniak and Przedniczek, 2021) were treated with jasmonic acid (JA) or the JA biosynthesis inhibitor - mefenamic acid (MEF). After application of the specific compound (3 h), the anthers were harvested, frozen in liquid nitrogen, and stored at -80 until use. The control consisted of untreated anthers. Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel). Isolations were performed from anthers collected from at least 90 randomly selected plants for each variant. The concentration and purity were measured with the NanoDrop ND-1000 spectrophotometer before further analysis. The integrity and quality of total RNA were assessed using the Agilent 2100 Bioanalyzer and agarose gel electrophoresis. Samples with RNA integrity number (RIN) > 8 were selected for further procedures. The cDNA library construction and the transcriptome sequencing were carried out by Genomed (Warsaw, Poland) on the HiSeq 2000 platform.