library_construction_protocol: Total RNA was extracted using RNA-easy Isolation Reagent(Vazyme), A total of 1 µg total RNA per sample was used as input material for thelncRNAlibrary preparation. Strand-specific libraries were generated using NEBNext®UltraTMRNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer'srecommendations and index codes were added to attribute sequences to eachsample.Briefly, ribosome RNA was depleted from total RNA. Fragmentation was carriedoutusing divalent cations under elevated temperature in NEBNext First StrandSynthesisReaction Buffer(5X). First strand cDNA was synthesized using randomhexamerprimer and M-MuLV Reverse Transcriptase (RNase H-). Second strandcDNAsynthesis was subsequently performed using DNA Polymerase I and RNase Hwiththeincorporation of dUTP instead of dTTP. Remaining overhangs were convertedintoblunt ends via exonuclease/polymerase activities. After adenylation of 3' ends of DNAfragments, NEBNext Adaptor with hairpin loop structure were ligated for hybridization.In order to select cDNA fragments of preferentially 150-200 bp in length, thelibraryfragments were purified with AMPure XP system.Then USER Enzyme (NEB, USA) was used to digest the second strand withsize-selected, adaptor-ligated cDNA at 37°C for 15 min followed by 5 minat 95°Cbefore PCR. Then PCR was performed with Phusion High-Fidelity DNApolymerase, Universal PCR primers and Index (X) Primer. And then, PCR products were purified(AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer2100 system.