design: The full-length 16S rRNA gene was amplified using universal bacterial primers, and amplicons were sequenced on the PacBio Sequel II platform (Pacific Biosciences, Menlo Park, CA, USA) to generate high-fidelity (HiFi) reads. Sequencing data were processed following standard PacBio workflows for full-length 16S rRNA gene analysis. High-quality reads were used for taxonomic assignment and downstream microbial community analyses.