design: This research proposed a novel bioremediation strategy involving two microorganisms: Enterobacter hormaechei and Bacillus subtilis. These microorganisms, isolated from tanneries in the Department of Quindio, demonstrated resistance and reduction activity against CrVI in preliminary tests. Initially, both microorganisms were immobilized together using sodium alginate and polyvinyl alcohol beads and subjected to an in vitro fluidized bead bioreactor with synthetic wastewater containing 100 ppm of CrVI. The treated water from the bioreactor was used in two in vivo models guppy fish Poecilia reticulata and tomato seeds Solanum lycopersicum L. to evaluate its toxicity. Finally, the genomes of these two microorganisms were analyzed to identify and study potential genes involved in the expression of CrVI resistance and reduction using bioinformatics tools.