library_construction_protocol: Briefly, 2.5× 104 mouse leukemia cells were treated and centrifuged at 600 g for 5 minutes at room temperature for each reaction. The cell pellet was resuspended in 50 μL lysis buffer containing 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, 3 mM MgCl₂, 0.1% NP-40, 0.1% Tween-20, and 0.02% digitonin, and centrifuged at 500 g for 10 minutes at 4°C. After centrifugation, the cell pellet was processed immediately for the transposition reaction, resuspended in 50 μL transposase mixture containing 25 μL 2× TD buffer, 22.5 μL nuclease-free water, and 2.5 μL Tn5 transposase. The mixture was incubated for 30 minutes at 37°C with mixing at 1,000 rpm. The transposed DNA was purified and amplified using NEBNext high-fidelity 2× PCR master mix with custom Nextera PCR primers. The transposed DNA was purified and amplified using NEBNext high-fidelity 2× PCR master mix with custom Nextera PCR primers. The optimal number of PCR cycles was determined by qPCR, and the DNA libraries were size-selected and sequenced on the NovaSeq 6000 platform.