library_construction_protocol: After deep anesthesia and decapitation, brains were dissected into coronal blocks, and bilateral medial prefrontal cortices were microdissected (N= 8-10 animals/ treatment group). Total RNA was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen), followed by DNase treatment. Total RNA was isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen), followed by DNase treatment. RNA quality was assessed with an Agilent TapeStation (Agilent  Technologies, USA), showing RIN values above 9.5. Libraries were prepared using the Illumina Stranded mRNA Prep kit (Illumina, Inc., USA, #20040534) and sequenced on an Illumina NextSeq 2000.