design: DNA isolation: Monarch Genomic DNA Purification kit (New England Biolabs); Yields quantified on Qubit3.0 (Thermo Fisher Scientific); Sequencing libraries prepared with NEBNext Ultra II FS DNA Library (New England Biolabs), Measured with BioAnalyzer 2100 (Agilent Technologies) and quantified with Qubit and KAPA before pooling. Sequencing done on NovaSeq 6000 at Broad Institute of MIT and Harvard (Walkup Sequencing at the Genomics Platform)