design: DNA was extracted using a Gentra Puregene Yeast/Bact DNA extraction kit (Qiagen, Australia). Sequencing libraries were prepared using the SQK-NBD114-24 kit and sequenced in FLO-PRO114M flow cell (Oxford Nanopore Technologies, Oxford, UK). Pod5 files were basecalled using Dorado (https://github.com/nanoporetech/dorado) with the Sup model v4.3.0.