design: DNA was extracted from 1 to 4 legs of the individual using the Wizard Genomic DNA Purification Kit (Promega) following the manufacturer's protocol. Individual sequence libraries were generated based on the genotype-by-sequencing (GBS) method described by Elshire et al. (2011). The libraries were constructed by EcoMol Consultoria (Piracicaba, SP, Brazil). The genomic DNA of individuals was digested with the restriction enzyme PstI (5-CTGCAG-3), linked to a specific barcode for each sample, and to a common adapter. Samples were sequenced at Laboratrio multiusurios centralizado de Genmica Funcional aplicada  Agropecuria e Agroenergia (GA3) (Departamento de Zootecnia, ESALQ/USP, Piracicaba, SP, Brazil) on a NexSeq 2000 platform (Illumina Inc., San Diego, CA, USA). Sequences were assembled using the ipyrad package (Eaton & Overcast 2020).