design: Sea water samples were collected from the Eastern Shore Islands area of Nova Scotia in spring, summer, and fall 2024 which underwent eDNA extraction and sequencing at the Aquatic Biotechnology Laboratory, Bedford Institute of Oceanography. 12S libraries targeting fish and COI libraries targeting general eukaryotic diversity were prepared and sequenced to help monitor biodiversity of this area. Paired beach seining was also conducted at the time of water sampling to compare fish catch data with eDNA results.