library_construction_protocol: Tissues were harvested at about 7 month of age post cardiac perfusion, snap-frozen, and single nuclei from cerebrum tissues were isolated using a gradient centrifugation-based method. Nuclei were pooled from 3 mice per genotype for each snRNAseq sample, and the PIPseq T20 3′ Single Cell RNA Kit v4.0 was used for cDNA library construction based on a particle-templated emulsification method. Libraries were pooled and sequenced using an Illumina NovaSeq 6000 instrument with 1% PhiX using an S4 flow cell (150 pair-end module).