design: RNA-seq of 5 pairs of adjacent normal tissues and CRC tumor tissues
library_construction_protocol: Between January 2021 and September 2021, a total of 5 pairs of adjacent normal tissues and CRC tumor tissues were collected at Suzhou Municipal Hospital. Written informed consent was secured from all participating patients prior to the commencement of the study. The study protocol, including all experiments, was reviewed and approved by the Ethics Committee of Suzhou Municipal Hospital.  Total RNA from 5 pairs of adjacent normal tissues and CRC tumor tissues was isolated using the PROTRIZOL reagent (PRN02, Proteinbio, Nanjing, China).  complementary DNA (cDNA) libraries were constructed and sequenced on the Illumina platform at Metware Biotechnology Co., Ltd (Wuhan, China)