design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 1420 day-old seedlings (from Hazera company), PEG-mediated CRISPRCas9 RNP transformation, UMI-DSBseq amplicon library targeting chr4;56475098;56475118;-_1st_rep (target sequence: TGGGATAATGTGTACGTCCTTGG) prepared for paired-end Illumina sequencing