library_construction_protocol: Mouse brains were rapidly collected and dissected, and the medial prefrontal cortex (mPFC) was identified using a mouse brain atlas and isolated by tissue punching (1-mm diameter). Total RNA was extracted using FreeZol reagent (Vazyme, #R711) following the manufacturer's instructions, and RNA concentration was measured using a NanoDrop spectrophotometer. RNA quality was assessed using an Agilent 5300 Bioanalyzer; samples meeting library construction criteria (OD260/280 = 1.8–2.2, OD260/230 ≥ 2.0, RIN ≥ 6.5, 28S:18S ≥ 1.0, and >1 µg total RNA) were used for sequencing library preparation. RNA-seq libraries were constructed using the Illumina Stranded mRNA Prep, Ligation kit according to the manufacturer's protocol, including poly(A) mRNA enrichment, cDNA synthesis, adapter ligation, size selection, and PCR amplification.