design: Artificially bred Mytilus edulis spat (shell length 1-2 cm) was set as Group YM Replicate 2 (blank control) and cultured in artificial seawater without antifouling agent under the same conditions as Group DM. Its soft tissue was collected at 12 h post-cultivation and stored in liquid nitrogen. Total RNA was extracted using FreeZol Reagent, and the RNA-Seq library was constructed via the same process as Group DM, aiming to provide a baseline gene expression profile for comparison with Group DM (refer to related documents).