library_construction_protocol: Total RNA was isolated using the Zymo directZol Mini Prep kit by homogenizing 100 ul of RLT cell suspension in 900 ul TriZOL reagent supplemented with 20 ul 1-Thioglycerol. 200 ng of total RNA were captured on oligo-dT beads and reverse transcribed directly on the beads. Libraries were prepared using the Lexogen SENSE mRNA-seq Library Prep Kit V2 (Lexogen, Greenland, NH, USA).