library_construction_protocol: Fly heads were obtained from samples of 50-60 flies through flash freezing with liquid nitrogen, followed by vortexing and separation through a series of a sieves. Fly heads were then suspended in homogenization buffer and nuclei released into solution through chemical and physical agitation of the cell membrane. Anti-GFP bound beads were then used to isolate GFP-positive nuclei from the whole head homogenate to represent the mushroom body-specific fraction (MB). DNA was isolated using a Qiagen MinElute Kit. ATAC-Seq libraries were generated by mixing purified DNA with custom Nextera primers and High-Fidelity PCR Mastermix (NEB) and amplified as per manufacturer's instructions. Final libraries were purified using a Qiagen PCR purification kit.