design: 1.DNA Preparation: Extracted high-molecular-weight (HMW) genomic DNA from strain 9107062.Digestion & Size Selection: Partially digested the DNA with Hind III and recovered fragments (50300 kb) via pulse-field gel electrophoresis (PFGE)3.Ligation & Transformation: Ligated fragments into the pCC1BAC vector and transformed them into EPI300 E. coli cells4.Library Quality: Produced 2,624 clones with an average insert size of 105 kb, achieving 8.3-fold coverage of the genome.