library_construction_protocol: Dneasy®  Plant  Maxi Kit (Qiagen) Leaf genomic DNA of tomato mbd5 mutants and WT were extracted using a DNeasy plant Mini kit (Qiagen), and then DNA library was treated with Illmina's standard DNA methylation analysis protocol and NEB Ultran II dna Library Prep kit. The samples were sequenced at the Genomics Core Facility of the Shanghai Centre for Plant Stress Biology, Chinese Academy of Sciences using Illumina nova6000.