design: A total amount of 0.2 g DNA per sample was used as input material for theDNAlibrary preparations. Briefly, genomic DNA sample was fragmented by CovarisLE220R-plus (Covaris, USA) to a size of 350 bp. Then DNA fragments wereendpolished, A-tailed, and ligated with the full-length adapter for Illumina sequencing,followed by further PCR amplification. PCR products were purified by AMPureXPsystem (Beckman Coulter, Beverly, USA). Subsequently, library quality wasassessedon the Agilent 5400 system (AATI) and quantified by real-time PCR (1.5nM). The qualified libraries were pooled and sequenced on Illumina platforms withPE150 strategy in Novogene Bioinformatics Technology Co., Ltd (Beijing,China),according to effective library concentration and the data amount required.