library_construction_protocol: Total RNAs were extracted using TRIzol Reagent (Invitrogen, cat. NO 15596026）following the methods. A total of 2 μg of high-quality RNA was used for the construction of sequencing libraries. KCTM Stranded mRNA Library Prep Kit for Illumina® (Catalog NO. DR08402, Wuhan Seqhealth Co., Ltd. China) following the manufacturer's instruction