library_construction_protocol: Total RNA from the different cell lines (HG3, PGA-1, Granta-519, MEC-1 and MO1043) was extracted using the mRNeasy Mini Kit (Qiagen) and quality control was performed using NanoDrop, Qubit 2.0, and Agilent 2100 Libraries were constructed using mRNA isolated by poly-T oligo-attached magnetic beads followed by random fragmentation and cDNA synthesis with random hexamers. Double-stranded cDNAs were end-repaired, adenylated, and ligated to adapters before PCR amplification