library_construction_protocol: Nuclei were extracted from 50,000 live cells and tagemented with Nextera Tn5 transposase (Illumina 20034197). Resulting DNA fragments were purified with the MinElute Kit (Qiagen 28004) Tagmented fragments were amplified with 10 cycles of PCR and purified with the MinElute Kit (Qiagen 28004)