library_construction_protocol: Sample washing with PBS containing 0.04% BSA, the cell pellets were re-suspended in PBS containing 0.04% BSA. Dissociated single cells were then stained with AO/PI for viability assessment using Countstar Fluorescence Cell Analyzer. RNA libraries were generated by 10xGenomics Single Cell 3' Library Gel Bead Kit V3 and prepared for sequencing using standard Illumina protocols