library_construction_protocol: Mice were euthanized with CO2, and whole brains removed. Brains were split down the midline, snap frozen in liquid nitrogen, and stored at -80C until snRNAseq protocol was initiated. snRNAseq was performed as a fee-for-service by the Brotman Baty Advanced Technology Lab (BAT-Lab) at the University of Washington. Libraries were constructed as described for sci-RNA-seq3 in Martin et al, DOI: 10.1038/s41596-022-00752-0