library_construction_protocol: For RNA extraction, 2.5 million neurons were pleated in 6 well plates (Thomas Scientific, #1198D71) and differentiated for 10-14 days. Neurons were treated with DMSO or 5 nM vincristine for 16 hours before RNA extraction. Neurons were washed once with PBS and harvested in 700 µL Qiazol (Qiagen, #79306) and immediately placed on ice. RNA extraction was performed using RNeasy Mini Kit (Qiagen, #74104) following the manufacturer's instructions. Columns were treated with RNase-Free DNase (Qiagen, #79254), and RNA was eluted in 25 µL of RNAse free water. Eluted RNA was separated into two tubes, one containing 2 µL, subsequently used for quality control and the other used for RNAseq. For RNA sequencing, 1-4 µg of total RNA samples were purified with PolyA extraction, and then purified mRNAs were constructed to RNA-Seq libraries with specific barcodes using Illumina TruSeq Stranded mRNA Library Prep Kit. All the RNA-Seq libraries were pooled together and sequenced using Illumina NovaSeq 6000 to generate approximately ~40 million 2x100 paired-end reads for each sample. The raw data were demultiplexed and analyzed further.