library_construction_protocol: Spleens (n = 4) were taken on day +14 and pooled. CD4+ T cells were enriched with MACS selection, stained with CD90.2 Pacific Blue, CD4 APC/Cy7, H2Dd AF647 and FACS sorted to P1 (Eomes- IL10-), P2 (Eomes+ IL10-) and P3 (Eomes+ IL10+) based on the expression of mCherry and GFP. Samples were resuspended at 1000 cells/uL and libraries  were  generated  using  the Chromium Single Cell 3' Reagent Kits User Guide (v3 Chemistry ). Final library quality was assessed using the Tapestation 4200 (Agilent, Santa Clara, CA) and libraries were quantified by Qubit dsDNA kit on Denovix( spectrometer/flurometer) .Pooled libraries were then subjected to paired-end Illumina sequencing according  to the  manufacturer's  protocol