design: 210^9 CFUs were pelleted and resuspended in enzymatic lysis buffer containing 10 mM Tris-HCl, 0.1 mM EDTA, 25 mg/ml lysozyme, and 0.3% lysostaphin, incubating for 2 h at 37 C. The bacterial suspensions were then bead-beaten with DEPC-treated 100-m zirconia beads at 4800 rpm for 1 min, repeated three times, with cooling periods after each beating. Total DNA extraction was performed using the Zymo DNA Miniprep Plus kit (Irvine, USA). The quantity and purity of the extracted DNA were assessed using a NanoDrop Microvolume Spectrophotometer and Qubit Fluorometric Quantification. The extracted DNA was submitted for WGS.