library_construction_protocol: The PaXgene Blood RNA kit was used to extract Total RNA from high yielding Gir (n=3), low yielding Gir (n=3), high yielding Shweta Kapila (n=3) and low yielding Shweta Kapila (n=3) cows Libraries were constructed using a modified NEBNext RNA Ultra II directional protocol. Ribosomal RNA (rRNA) was removed using biotinylated oligos and rRNA removal beads and RNA was subsequently fragmented using divalent cations at elevated temperatures. The first strand of cDNA was synthesized using random hexamers and reverse transcriptase, followed by double-stranded cDNA synthesis. Strand specificity was maintained by replacing thymine with uracil and selectively digesting the second strand using the USER enzyme. Enriched cDNA was indexed through limited-cycle PCR, followed by AMPure bead purification.