design: 100 mL of sample was centrifuged at 4300x g for 10 min at 4 C. The supernatant was discarded and the resulting pellet resuspended in 1.5 mL of nuclease-free water (Invitrogen, Waltham, USA). For DNA isolation, 1 mL of the resuspended pellet was transferred to a ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) (Zymo Research, Freiburg, Germany) and stored on ice immediately. Mechanical disruption was performed with a tissue homogenizer (Precellys 24, bertin technologies, Montigny-le-Bretonneux, France), for two times 30 sec at 4000 rpm and tubes immediately stored on ice again in between and after mechanical disruption. After centrifugation for 10 min at 10,000x g and 4 C, 1 mL of the supernatant was used for DNA isolation using a ZymoBiomics DNA Microprep Kit according to the manufacturers advice (Zymo Research, Freiburg, Germany), with an elution volume of 100L nuclease-free water. DNA library preparation was performed with the Nextera XT kit (Illumina, San Diego, USA) according to the manufacturers advice on the Biomek FXP pipetting robot (Beckman Coulter, Pasadena, USA) with an input of 10 ng.