design: Concurrent control of airway basal stem cell differentiation transition and the mesenchymal-to-epithelial transition by the jamming
library_construction_protocol: Cells were grown according to the protocol in DOI 10.1385/1-59259-861-7:183 Passage 2 primary human bronchial epithelial (HBE) cells  were cultured in air-liquid interface (ALI) culture for up to 14 days in culture. HBE cells were grown normally according to the growth protocol above and frozen on ALI days 0, 1, 7, and 14 to allow for RNA extraction. Total RNA was isolated from HBE cells frozen on ALI days 0, 1, 7, and 14 using the TRIzol Plus RNA Purification Kit (Invitrogen) Sequencing libraries were constructed with a Watchmaker Genomics kit.