design: Total non-enriched extracted DNA from each sample was subjected to short-read Illumina library preparation using the Illumina DNA Prep protocol per manufacturers instructions. After pooling libraries, a KAPA qPCR Library Quantification kit (Roche, Basel, Switzerland) was used to confirm functionality of the barcoded pool, and a MiSeq Nano run (V2 chemistry, 2x150bp paired-end reads) was used to assess final barcode balance. The final multiplexed library was sequenced by the University of Minnesota Genomics Center on a single lane of a NovaSeq6000 (Illumina, San Diego, CA, USA) with S4 cell chemistry to obtain 2x150 paired-end reads.