library_construction_protocol: For RNA-seq, total RNA was extracted using Trizol reagent kit according to the manufacturer's protocol. For RNA-seq, mRNA was enriched with magnetic beads containing Oligo (dT). Subsequently, a fragmentation buffer was added to break the mRNA into short fragments. Using mRNA as a template, a single strand of cDNA was synthesized using six base random primers. Then, buffer, dNTPs, and DNA polymerase I and RNase H were added to synthesize a double stranded cDNA. The double stranded cDNA was purified using AMPure XP beads. The purified double stranded cDNA is first subjected to end repair, followed by adding an A-tail and connecting to a sequencing adapter, followed by AMPure XP beads for fragment size selection. Finally, PCR amplification was performed and the PCR product was purified using AMPure XP beads to obtain the final sequencing library.