library_construction_protocol: Spinal cord cells were extracted from the trunks of wildtype zebrafish larvae expressing the olig2:egfp transgene at 36 and 48 hpf. Cells were sorted for EGFP prior to nuclei isolation. Single-cell sequencing was performed using the Chromium Next Gen Single Cell Multiome ATAC and Gene Expression Library and Gel Bead Kit (10X Genomics) and the Chromium X. Libraries were sequenced on an Illumina Novaseq 6000