design: Approximately 100 ng of DNA was sonicated to 100250 bp fragments and prepared into sequencing libraries using 12 PCR cycles with the xGen cfDNA & FFPE DNA library prep kit (Cat# 10010207, Integrated DNA Technologies, Coralville, Iowa), which includes unique molecular identifiers (UMIs), following the manufacturers instructions. Library DNA was then subjected to the JBS HBV-targeted NGS assay (JBS Science Inc, Doylestown, PA).