library_construction_protocol: Five oocytes per sample were collected from the wildtype and Psma7 cKO female mice for RNA-seq. Each sample was immediately lysed with 4 µL lysis buffer (containing 0.2% Triton X-100, RNase inhibitor, dNTPs, oligo-dT primers, and 0.2 µL 1:1000 ERCC mRNA spike-in dilution for mouse oocyte samples). RNA libraries for RNA-seq were prepared using the published Smart-seq2 method.