library_construction_protocol: Extracted islets from mice were pooled and dissociated into single cell suspension with 0.05 % trypsin (Cat# 25300062, Gibco) at 37 °C for 15 min, with shaking up and down once in every 2 min. And the digestion was stopped with RPMI1640 supplemented with 10 % FBS. Then cells were filtering with 30 μm cell strainers and centrifuged at 500 rpm for 5 min and resuspended with RPMI1640 (Cat# C11875500BT, Gibco) supplemented with 10 % FBS. The prepared single cell suspension, 10× barcode gel magnetic bead and oil were loaded into 10× Genomics Chromium and sort into different cells of Chromium Chip (Cat# 1000120, 10× Genomics) to formed GEMs (Gel Bead-in-Emulsions), in which one droplet contain a gel magnetic bead with barcode and a cell. Chromium Next GEM Single Cell 3ʹ GEM Kit v3.1 (Cat# 1000123, 10× Genomics) were used for reverse transcription in the individual GEM and barcoded cDNA which purified by magnetic beads were amplified by PCR reaction. Also, Chromium Next GEM Single Cell 3ʹ Library Kit v3.1 (Cat# 1000157, 10× Genomics) were used for scRNA-seq library preparation according to the manufacturer's protocols.