library_construction_protocol: Cells were bound to Concanavalin A-coated magnetic beads, then permeabilized with digitonin. Myc-primary antibody and pAG-MNAse were incubated with cells, and calcium choloride is added to activate the pAG-MNase to initiate DNA digestion. Cleaved DNA was isolated and purified by addng supernatent to spin column DNA Libraries were prepared using SimpleChIP® ChIP-seq DNA Library Prep Kit for Illumina® #56795 (Cell Signaling Technology).