library_construction_protocol: For ATAC-seq,nuclei were pelleted at 500 g for 10 min, then resuspended in transposase reaction mix (Illumina) and incubated at 37℃ for 30 min. DNA was purified using a MinElute kit (QIAGEN). For ATAC-seq, the DNA fragments were amplified in a 12-cycle PCR to add unique indices for sequencing and purified using a MinElutekit.