design: ~2g of fecal sample was collected in 50ml falcon tubes. To this 12ml of SM buffer was added and then homogenized by manual inverting the contents. The homogenate was then centrifuges for 10 minutes at 4700 rpm. The supernatant was passed through a 0.45um filter then a 0.22um filter into a fresh 15 ml conical centrifuge tube and final volume adjusted to 10ml. Polyethylene glycol (PEG) was added at 15% (1.5g) to all samples and were manually inverted until contents were fully suspended. This solution was kept overnight at 4 degrees celsius. From the filtrate with PEG, 1.6 ml was transferred to a 1.7ml microcentrifuge tube and centrifuged at 8000 rpm for 30 minutes. The supernatant was decanted and the remaining pellets were resuspended with 500ul of the filtrate before PEG was added. 200ul was then used in the protocol for the High Pure viral nucleic acid kit (Roche Diagnostics, USA). TempliPhi 500 kit (Cytiva, USA) was used subsequently to perform a rolling-circle amplification of the viral DNA extract. TrueSeq Nano Library Prep Kit was used to create Illumina sequencing library which was then sequenced on an Illumina HiSeq 4000 sequencer.