library_construction_protocol: Primary bone marrow macrophages (BMMs) were harvested from the femurs and tibiae of 2-month-old male and female C57Bl/6J mice. Femurs and tibiae were dissected, and adherent tissue was removed. The ends of the bones were cut, and the marrow was flushed from the inner compartments. Mandibles were dissected in two portions, a right and left side, and adherent tissue was removed. The molar teeth of each mandibular section were extracted, and tooth sockets were flushed. The condylar and incisal portions of the mandible were cut, and the marrow space was flushed from the inner compartments. From both the mandibular and femoral derived bone marrow cells were positively selected using CD11b microbeads and magnetic column isolation (Miltenyi Biotec, catalog #130-097-142) following manufacturer's instructions. Briefly, flushed cells were pelleted and resuspended in MACS buffer (phosphate–buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin, 2 mM EDTA). The cells then were incubated with CD11b microbeads for 15 minutes. The cells were then rinsed for 10 mins with MACS buffer. Following incubation, the cells were applied to the MACS column in a magnetic field. The columns were rinsed three times. The columns were then removed from magnetic field and flushed with 5 mL of MACS buffer to elute bound CD11b+ cells. Six samples (3 femoral and 3 mandibular) were submitted for single-cell RNA sequencing. Single cell captures targeting 10,000 cells each were performed by 10X Genomics ST G chip for 3' gene expression at UMN Genomic Center.