design: RNA-seq analysis of whole-brain samples from mice orally administered with Theogallin
library_construction_protocol: Whole brain was collected from 13-week-old male mice that were orally administered theogallin for 1 weeks. Brains were rapidly dissected, snap-frozen in liquid nitrogen, and stored at −80°C until RNA extraction. Total RNA was extracted from whole brain tissues using TRIzol reagent (Thermo Fisher Scientific) according to the manufacturer's instructions. RNA concentration and integrity were assessed with a NanoDrop spectrophotometer and Agilent Bioanalyzer. libraries were prepared with Ion AmpliSeq Transcriptome Mouse Gene Expression Kit (Thermo Fisher Scientific) according to the manufacturer's instructions.