library_construction_protocol: Lung metastases from NeuN mice (NT2.5-LM) under six different treatment conditions (V, E, EP, EC, EPC, PC) were dissected from lungs using fine-tipped scissors under sterile conditions inside a biosafety level 2 cabinet and digested using the Miltenyi mouse tumor dissociation kit (Miltenyi, cat. # 130-096-730) in combination with the Miltenyi Octo dissociator, following the manufacturer's directions. For library preparation, 10× Genomics Chromium Single-Cell 3′ RNA-seq kits v3 were used. Gene expression libraries were prepared according to the manufacturer's protocol.   Each batch had an approximately equal assortment of samples from each treatment group to reduce technical biases. Illumina HiSeqX Ten or NovaSeq was used to generate approximately 6.5 billion total reads.