library_construction_protocol: The cells were filtered through a 30-µm cell strainer and were re-suspended in 60 µL PBS containing 0.04% BSA to achieve 1,000 cells µl-1 for capture on the 10× Genomics Chromium controller. We obtained single-cell full-length cDNAs using the 10× Genomics platform, and then used the HIT-scISOseq method for library construction and sequencing.