design: Transcriptome sequencing of Eurygaster integriceps nervous system and fat body tissues. RNA-seq libraries were prepared using the Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold for rRNA depletion. Paired-end sequencing (2  101 bp) was performed on the Illumina NovaSeq 6000 platform to study tissue-specific gene expression during diapause.