library_construction_protocol: Genomic DNA was extracted by DNaeasy Extraction Kit (Qiagen, Cat# E1941). Genomic DNA was amplified by PCR using Q5 DNA polymerase. Barcodes for Illumina sequencing were added via a second PCR step, using 1 μl of the first PCR as a template. Barcoded PCR products were pooled according to amplicon and quantified by Qubit dsDNA HS assay kit (Thermo Fisher).