design: Poly(A)+ mRNA was enriched from total RNA using oligo(dT)-coupled magnetic beads, followed by fragmentation under optimized conditions. Double-stranded cDNA was synthesized from the fragmented mRNA, subjected to end repair and A-tailing, and then ligated with Illumina sequencing adapters. The resulting libraries were size-selected (300500 bp), PCR-amplified, and rigorously quality-checked before being sequenced on the Illumina HiSeq 2000 platform (San Diego, CA, USA).