design: The sequencing library was prepared using the DNBSEQ short-read library preparation protocol. Genomic DNA was first fragmented, followed by size selection to obtain appropriately sized fragments. The fragments then underwent end repair and the addition of an "A" tail to facilitate adapter ligation. After adapter ligation, a second size selection step was performed to ensure proper fragment distribution. The library was then amplified using PCR to enrich for successfully ligated fragments. Finally, quality control checks were conducted to assess the integrity and quality of the constructed library before sequencing.