design: Genotyping-by-sequencing (GBS) approach applied to 378 Pistacia atlantica genotypes from multiple populations (AA, BB, BZ, MN, MO, MJ, SC, KH, ...) from Iran for population genomics and genetic diversity analysis. Genomic DNA was digested with restriction enzymes and sequenced at Genomics lab of IPK (Germany) to identify genome-wide SNP markers for phylogeographic and population structure studies.