design: ATAC-seq assays (NYH209 and B7268 strains) were performed by Quick Biology, Inc. Briefly, nuclei isolation was performed from 2 million cells from each strain, then, samples were exposed to the Tn5 transposase (Illumina) for 30 minutes at 37C. Tn5 transposase acts as a homodimer to simultaneously fragment the chromatin and insert the necessary adapters for downstream amplification and sequencing. Immediately after incubation with Tn5 the samples were purified using a commercial purification kit. This was followed by a 12-cycle PCR to amplify the DNA fragments and a second round of purification