library_construction_protocol: FFPE tissue blocks were made from tumor-harboring mouse brain tissues immediately post-euthanasia and cardiac perfusion with PBS, and 5 µm-thick sections were mounted onto each spatially barcoded capture area of the Visium Spatial Gene Expression slide. Spatial transcriptome sequencing libraries were prepared with 10x Genomics Visium Spatial Gene Expression Slides & Reagent Kit according to manufacturer's protocol.