library_construction_protocol: Total RNA was isolated using the RNeasy Mini Kit (QIAGEN, Cat# 74104) following the manufacturer's instructions. RNA quality was assessed using an Agilent 2100 TapeStation. Stranded mRNA-seq libraries were prepared with the KAPA mRNA HyperPrep Kit (Roche, KK8581), according to the provided protocol. Sequencing was performed on an Illumina NextSeq 500 platform using a 75-base single-end high-output run configuration. Sample collection and processing were randomized across groups and performed on the same day to minimize batch effects.