design: Small segments from the middle section of the Physaloptera sp. sample were homogenized in 400 ul of SM buffer using a pestle. The homogenate was centrifuged at 10000 x g for 10 mins. 200ul of the supernatant was used for total RNA extraction using High Pure Viral Nucleic Acid kit. 2 ul of the using the QIAwave RNA plus mini kit. The RNA was used to prepare Illumina library using the Illumina Stranded Total RNA Prep with Ribo-Zero Plus Microbiome kit (Illumina, USA) This was sequenced at Psomagen Inc (USA) on an Illumina NovaseqX Psomagen Inc (USA).