design: Whole-genome resequencing of an ilamycin E-resistant Mycobacterium abscessus strain.  Genomic DNA was extracted from pure culture and rigorously quality-controlled.  Libraries were constructed by fragmenting DNA, end-repairing, adenylating 3' ends,  and ligating Illumina adapters with unique indexes.  Size selection was performed using magnetic beads, followed by PCR amplification.  Final libraries were pooled and sequenced on an Illumina HiSeq platform (paired-end, 2150 bp) to investigate genomic determinants of ilamycin E resistance.  Strict quality control was applied at each step (DNA extraction, library preparation, and sequencing)  to ensure data reliability.