library_construction_protocol: Total RNA was isolated with TRIzol reagent (Ambion) from each individual sample (the whole larvae). RNA samples from the same group (12-16 individuals) have been combined into mixtures to obtain four RNA pools. The library was examined using Qubit and real-time PCR for quantification and size distribution detection. Quantified libraries were pooled in equimolar amounts and sequenced on Illumina NovaSeq 6000 High Throughput Sequencer using a NovaSeq 6000 Reagent Kit v1.5 in 150 bp paired-end reads at Novogen company (China).