library_construction_protocol: Total RNA was extracted using TRIzol reagent and treated with DNase I to remove genomic DNA. mRNA was enriched from total RNA using Oligo(dT) magnetic beads, randomly fragmented, and reverse-transcribed into double-stranded cDNA. The cDNA was then subjected to end repair, A-tailing, adaptor ligation, and PCR amplification. Qualified libraries (verified by Qubit quantification and Agilent fragment analysis) were sequenced on an Illumina platform (conducted by Novogene, Beijing, China).