library_construction_protocol: Total RNA was extracted using the Total RNA Extractor (Trizol) kit (B511311, Sangon, China) according to the manufacturer's protocol. A total amount of 1 μg RNA per sample was used for the construction of the sequencing library . Sequencing libraries were generated using VAHTSTM mRNA-seq V2 Library Prep Kit for Illumina® following manufacturer's recommendations and index codes were added to attribute sequences to each sample.