design: The 20K SMRT Bell library was constructed using the SMRT bell TM Template kit (version 1.0), and the electrophoretically detected DNA samples were interrupted by Covaris g-TUBE to form the target fragments of the required size for the library construction, and then DNA damage repair and end repair were performed, and DNA adhesion enzyme was used to connect the hairpin junctions at both ends of the DNA fragments, and the DNA fragments were purified using AMpure PB magnetic beads to screen for specific size fragments.The DNA fragments were purified using AMpure PB magnetic beads, the BluePipin fragments were used to screen for specific sizes, and the SMRT Bell library was screened for concentration using AMpure PB magnetic beads, followed by repair of the DNA damage, and the SMRT Bell library was purified again using AMpure PB magnetic beads, and the constructed library wasThe constructed library was quantified by Qubit concentration, and the size of insert fragments was detected by Agilent 2100, and finally sequenced by PacBio platform. *** Translated with www.DeepL.com/Translator (free version) ***