design: WGS libraries were prepared individually for each sample using the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific, USA). Genomic DNA was sonicated into fragments, end-repaired, 3'-A tailed and ligated with Y-adaptors, followed by PCR amplification and AMPure XP beads-based selection of ~470 bp fragments. Quantified by Qubit 4 Fluorometer, the fragments were denatured, circularized and amplified into DNBs (>300 copies) via rolling circle replication. DNBs were loaded onto nanoarrays and sequenced on the DNBSEQ-T7 platform with PE152 mode.