library_construction_protocol: Approximately 2 x 106 Panc-01WT or Panc-01KO cells were crosslinked in triplicate with 1% paraformaldehyde in PBS buffer for 15 min and stopped by the addition of 1.25 M glycine for 5 minutes at room temperature. Cells were lysed 35 and sonicated using Bioruptor Plus (Diagenode) for 10 min with 5s on and 5s off pulses in low power output at 4ºC. Optimal chromatin fragmentation of 400-300 bp was verified in reversed cross-linked samples on a TapeStation 2200 system (Agilent Technologies). Library preparation was performed with the Omni-ATAC method. Once the final library was obtained, a purification step using AMPure XP beads was done before concentration and fragment size assessment. A quality control step consistent of size distribution and yield estimation is applied before the library sequencing.