design: RNA was extracted using the Zymo Direct-zol Microprep R2060 Kit with DNase removal steps, eluted in 15ul water. After quality assurance using the Agilent Tapestation, libraries were prepared using an Illumina Stranded mRNA library kit, and sequenced at The Huck Institutes Genomics Core Facility using either the NextSeq 2000 or Nextseq P4 XLEAP sequencers paired-end