library_construction_protocol: Leaf segments from the above conditions were flash frozen in liquid nitrogen and stored at -80°C. Tissue was ground to a powder in a bead mill (30s, 6m/s), and total RNA was extracted with a Qiagen RNeasy Plant Mini Kit. Total RNA was sent to Génome Québec (Montreal, Quebec) for library construction and sequencing, using Nextera XT library preparation and sequencing of 100bp paired-end reads on a NovaSeq 6000.