design: The procedure was conducted in strict accordance with the standard protocol provided by the sequencing service company, encompassing sample quality control, library construction, library quality assessment, and library sequencing. After confirming that the sample genomic DNA passed quality inspection, it was fragmented via mechanical shearing (ultrasonication). The fragmented DNA was then subjected to a series of steps including fragment purification, end repair, 6'-end A-tailing, and adapter ligation. Following this, size selection was performed using agarose gel electrophoresis. The selected fragments were subsequently amplified by PCR to generate the final sequencing library. The constructed library underwent rigorous quality control, and only those libraries passing the quality assessment were proceeded to the sequencing stage.