library_construction_protocol: For RAP-seq experiments, 25 million primary CD8 T cells, followed by 2mM DSG and 3% formaldehyde was used for crosslinking. Lysed whole cells were fractionated to obtain intact nuclei. Nuclei were lysed and chromatin was fractionated with a combination of sonication and TURBO DNase diestion. Lysate was incubated with antisense single stranded 5' biotinylated DNA probes tiling the entire lnc13 transcript but does not bind to Il18rap. Streptavidin-magnetic beads were used to enrich for lnc13 and bound chromatin was eluted by RNaseH digestion. NEBNext Ultra II DNA Library Prep Kit was used for RAP-seq samples.