library_construction_protocol: For multiplex analysis, cells were barcoded using BioLegend Hashtag DNA. Single-cell capture and reverse transcription were performed using the BD Rhapsody system cDNA amplification was carried out using the Terminator-assisted solid-phase cDNA amplification sequencing (TAS-Seq) method (Shichino et al., Commun. Biol., 2022), in collaboration with ImmunoGeneTeqs Inc.