design: This sample represents NE201KT, a transposon insertion mutant of Staphylococcus aureus strain JE2 from the Nebraska transposon mutant library. The mutant carries a disruption in the phi2 int gene via a transposon harbouring the kanamycin resistance gene aphA-3, as well as pT181. Whole genome sequencing was performed using Illumina paired-end technology to confirm the transposon insertion site and plasmid.