library_construction_protocol: Spleens were harvested from infected mice and macerated through a 100µm Nytex filter, washed with DMEM and erythrocyte lysis was performed using a hypotonic ACK medium. Lysis was stopped by adding DMEM containing 10% FCS. Cells were counted using a Neubauer counting chamber  stained with flurorescently labelled antibodies against CD8a,  CD44 as well as a fixable live/dead dye and multimers loaded with either gp33 or np396 and conjugated to different flurochromes. Single, live reporter+ CD8 T cells reacting to either of the multimers were sorted and used for library generation. Gene expression and T-cell receptor V(D)J libraries were prepared by using the Chromium Next GEM Single Cell 5' Reagent Kit v2 (10X Genomics), Chromium Single Cell Mouse TCR Amplification Kit (10X Genomics) and Chromium Next GEM Chip K Single Cell Kit (10X Genomics) following the manufacturer's protocol (CG000331 Rev E). Dual Index Kit TT Set A (10X Genomics) was used for multiplexing (i7 and i5 index read, 10bp). The samples were sequenced in a paired-end run (read 1: 26bp, read 2: 90bp) on a NovaSeq6000 platform using S1 v1.5 (100 cycles) sequencing kits (Illumina). Bcl2fastq software (v2.20.0.422) was used for demultiplexing and generating .fastq files.  The libraries were sequenced in a paired-end run (read 1: 26bp, read 2: 90bp) on a NovaSeq6000 platform using S1 v1.5 (100 cycles) sequencing kits (Illumina). Bcl2fastq software (v2.20.0.422) was used for demultiplexing and generating .fastq files.