library_construction_protocol: Tissues (51–55 mg) were minced, fixed in fixation buffer for 24 hours at 4 °C, and dissociated using the gentleMACS Octo Dissociator (Miltenyi Biotec) following the Chromium Next GEM Fixed RNA Sample Preparation Kit protocol (10x Genomics). Fixed single-cell suspensions were hybridized with probe barcodes using the Chromium Fixed RNA Kit, Human Transcriptome (10x Genomics). Equal numbers of cells from each sample were pooled prior to library construction. GEMs were generated using the Chromium iX and Chip Q. Probe ligation, pre-amplification, and library construction were performed according to the manufacturer's protocol. Libraries were indexed using the Dual Index Kit TS Set A and sequenced on an Illumina NovaSeq 6000 (paired-end 150 bp), generating approximately 1.6 billion reads in total.