design: This sample represents NE492, a transposon insertion mutant of Staphylococcus aureus strain JE2 from the Nebraska transposon mutant library, received directly from the distributor, BEI. The mutant is intended to carry a disruption in the SAUSA300_1793 gene. Whole genome sequencing was performed using Illumina paired-end technology to confirm the transposon insertion site. This identified a disruption in the sdrH gene, rather than SAUSA300_1793.