library_construction_protocol: 3.5 x 108 parasites were fixed with 0.8% formaldehyde for 20 min at room temperature. Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 sec on, 30 sec off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation, and SDS in the lysate supernatants was diluted to 0.07%. (Input) samples were taken before incubating the rest of the cell lysates overnight with 10 μg rabbit anti-GFP antibody (Thermo Fisher Scientific A-11122) and Protein G Dynabeads. The beads were washed, and the DNA eluted from them was treated with RNase and Proteinase K. DNA was then purified using a QIAquick PCR purification kit (Qiagen). libraries were prepared using NEXTflex barcoded adapters (Bio Scientific). The libraries were sequence on Illumina NextSeq (Western General Hospital, Edinburgh). In all cases, 75-bp paired-end sequencing was performed. Our subsequent analyses were based on two replicates for all TALEs.