library_construction_protocol: White blood cells from breast cancer patients or healthy controls were isolated from peripheral blood samples and sorted using FACS Patient cells were labelled with hashtag barcode oligo and libraries were prepared per manufacturer's instructions. Briefly, cells were loaded onto a 10X Chromium controller, and library was constructed using 10X Chromium Next GEM Single Cell 5' Library and Geal Bead kits (v1.1)