design: A total amount of ~100ng gDNA per sample was used as input material for the DNAsample preparations. Sequencing libraries were generated using NEBNextR UltraDNA Library Prep Kit for Illumina (NEB, USA) following manufacturersrecommendations and index codes were added to attribute sequences to each sample.Briefly, the DNA sample was fragmented by sonication to a size of 350bp, then DNAfragments were end-polished, Atailed, and ligated with the full-length adaptor forIllumina sequencing with further PCR amplification. At last, PCR products werepurified (AMPure XP system) and libraries were analysed for size distribution byAgilent2100 Bioanalyzer and quantified using real-time PCR.