design: The initial concentration of DNA  was evaluated using the Qubit dsDNA HS Assay Kit (Life Technologies). 50ng of DNA was used to prepare the libraries using Illumina DNA Prep, (M) Tagmentation library preparation kit (Illumina) following the manufacturer user guide.  The samples underwent simultaneous fragmentation and addition of adapter sequences. These adapters are utilized during a limited-cycle PCR in which unique indices are added to the sample. Following library preparation, the final concentration of the libraries (Table 1) was measured using the Qubit dsDNA HS Assay Kit (Life Technologies), and the average library size was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies).  The libraries were then pooled in equimolar ratios of 0.6nM and sequenced paired end for 500 cycles using the NovaSeq 6000 system (Illumina).