design: One way or another: Differential impact of pharmacologic inhibitors on extracellular vesicles derived from resting neutrophils
library_construction_protocol: For RNA sequencing, EVs were isolated from three biological replicates. The dry EV pellets were resuspended in 10 μL sterile distilled water (nuclease-free), snap frozen, and stored at -80°C until processing within 1 week. Total RNA was extracted using a Qiagen RNA extraction kit, following the manufacturer's instruction. Small RNA libraries were prepared using the Qiagen miRNA Library Prep Kit, which applies bead-based size selection to enrich fragments between 18–40 nt. 