library_construction_protocol: Aortic CD45+ cells were isolated as previously described (PMID: 33731931). Briefly, aortas from male Ldlr-/- mice (n = 7) fed a high-fat diet for 12 weeks were pooled and digested with an enzyme mixture. Aortic single cells were stained with anti-CD45 antibody and DAPI. DAPI-/CD45+ cells were sorted by FACS and collected for scRNA-seq. scRNA-seq libraries were prepared using a Chromium Single Cell 3' Reagent kit (10x Genomics) according to the manufacturer's instructions.