library_construction_protocol: Total RNA from esophageal biopsies was isolated from 5 scrolls per sample of 10-micron, formalin-fixed, paraffin-embedded tissue with the High Pure FFPET RNA isolation kit (Roche Diagnostics) that includes on-column DNase digestion RNA libraries for RNA-seq were prepared using Illumina TruSeq RNA Exome library kit following manufacturer's protocols