design: 1. DNA was extracted from muscle tissue of an adult male Sarotherodon galilaeus and used to construct a Hi-C library using the in situ Hi-C method.2. Library concentration and insert size were assessed using Qubit 2.0 and Agilent 2100, while effective concentration was measured by Q-PCR.3. The Hi-C library was sequenced on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads (PE150).