design: Library construction method: Illumina TruSeq Stranded mRNA (polyA)Sequencing: Samples were sequenced on NovaSeq6000 (NovaSeq Control Software 1.8.0/RTA v3.4.4) with a 151nt(Read1)-19nt(Index1)-10nt(Index2)-151nt(Read2) setup using 'NovaSeqXp' workflow in 'S4' mode flowcell. The Bcl to FastQ conversion was performed using bcl2fastq_v2.20.0.422 from the CASAVA software suite. The quality scale used is Sanger / phred33 / Illumina 1.8+.Data Flow: Raw sequencing data is demultiplexed and converted to FastQ on site before being transferred securely to UPPMAX for delivery.Data Processing: To ensure that all sequenced data meets our guarantee of data quality and quantity, a number of standardised bioinformatics quality control checks are performed before delivery. These include checking the yield, sequence read quality and cross-sample contamination.