design: SUMOylation of rice DELLA SLR1 modulates transcriptional responses and improves yield under salt stress.
library_construction_protocol: Shoots from 14-day seedlings of Nipponbare, SLR1-OX #2, and SUMO1SLR1-OX #2 in control and subjected to salt stress for 8h (NaCl 120 mM) were collected and frozen in liquid nitrogen. Dehusked rice seeds were surface sterilized with 70% ethanol for 10 min and further sterilized with 25% bleach (commercial bleach) for 15 min, then washed five times in sterile water. The seeds were placed in hydroponics with half-strength Murashige & Skoog Medium and MES buffer 250 mg/L (Duchefa) with pH adjusted to 5.2. Seedlings were grown for 14 days before the salt stress assay. Then, the seedlings were exposed to salt stress for 8h (NaCl 120 mM). Dehusked rice seeds were surface sterilized with 70% ethanol for 10 min and further sterilized with 25% bleach (commercial bleach) for 15 min, then washed five times in sterile water. The seeds were placed in hydroponics with half-strength Murashige & Skoog Medium and MES buffer 250 mg/L (Duchefa) with pH adjusted to 5.2. Seedlings were grown for 14 days before the salt stress assay. Then, the seedlings were exposed to salt stress for 8h (NaCl 120 mM). RNA was extracted from a total of 100 mg of ground shoot sample with a Direct-zol kit (Zymo Research) according to the manufacturer's protocol with in-column DNAse treatment. The cDNA libraries were obtained from 10 ng total RNA and using an optimized SMARTSeq2 protocol.