design: This study aims to analyze the microbial community composition and functional potential in samples from patients using the next-generation metagenomic sequencing. DNA was extracted from throat swab samples using HiPure Bacterial DNA kit. A shotgun metagenomic library was prepared using VAHTS Universal DNA Library Prep kit for Illumina V3, followed by sequencing on an Illumina NovaSeq 6000 platform with paired-end 150 bp reads.