library_construction_protocol: Total RNA was isolated from frozen samples using the TRIZOL method;To remove genomic DNA, RNA samples were treated with DNase I and RiboLock RNAse inhibitor (both Thermo Fisher Scientific) as per the manufacturer's instructions. Total RNA was then purified using the Monarch RNA Cleanup Kit (New England BioLabs, #T2030L). mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads. Following fragmentation, first-strand cDNA was synthesized using random hexamer primers, followed by second-strand synthesis. The library preparation included end repair, A-tailing, adapter ligation, size selection, amplification, and purification.