design: Total RNA was isolated from TFCP2L1-knockout Ishikawa human endometrial epithelial cells. mRNA was enriched using Oligo(dT) magnetic beads to capture poly(A)-tailed transcripts. Enriched mRNA was fragmented into 250-300 bp with NEB Fragmentation Buffer. cDNA was synthesized using random primers and M-MuLV reverse transcriptase. For strand-specific libraries, dUTP was used in second-strand synthesis, followed by USER enzyme degradation prior to PCR. Double-stranded cDNA was end-repaired, A-tailed, ligated to adapters, size-selected (250-300 bp) with AMPure XP beads, PCR-amplified, and purified. Libraries were prepared with NEBNext Ultra RNA Library Prep Kit (standard) or NEBNext Ultra Directional RNA Library Prep Kit (strand-specific) for Illumina sequencing.