library_construction_protocol: Total RNAs were extracted using TRIzol Reagent (Invitrogen, cat. NO 15596026） Sample 1-21: Briefly, mRNA was enriched using oligo-dT magnetic beads, fragmented, and subjected to quality control. First- and second-strand cDNA synthesis was followed by adenylation of 3′ ends and adapter ligation. For sequencing, circular DNA was amplified into DNA nanoballs (DNBs) via rolling circle amplification and loaded onto patterned nanoarrays.