design: For the creation of the PacBio library, an unamplified library was generated using the Pacific Biosciences standard template preparation protocol for creating >10 kb libraries. Genomic DNA (10 g)  was sheared using Covaris g-Tubes to generate >10kb fragments (Covaris, Inc., Woburn, MA, USA). The sheared DNA fragments were then prepared according to the Pacific Biosciences SMRTbell template preparation kit guidelines (Pacific Biosciences, Menlo Park, CA, USA).