library_construction_protocol: Single-nucleus isolation was performed using the Chromium Nuclei Isolation Kit 16 rxns (10x Genomics) according to the manufacturer's instructions. Then, 9 μl aliquot of the nuclear suspension was mixed with 1 μl of trypan blue, and the concentration of nuclei was determined under a microscope using a hemocytometer, with the count averaged over three replicates. The concentration of the nuclei suspension was adjusted to approximately 1,000 nuclei/μl by adding Wash and Resuspension Buffer. library was performed accordong to the manufacter's instructions (Chromium Next GEM Single Cell 3ʹ Reagent Kits v.3.1, 10x Genomics).