library_construction_protocol: Cells were treated with 1% PFA at 37 °C for 15 minutes, followed by the addition of 0.125 M glycine to quench the fixation reaction, with incubation for 5 minutes at room temperature. The cells were then washed with cold PBS supplemented with 1 mM PMSF and lysed using ChIP SDS lysis buffer (50 mM Tris-HCl, pH 8.1, 10 mM EDTA, 1% SDS) containing protease and phosphatase inhibitors for 15 minutes on ice. DNA fragmentation within the desired range of 200–800 base pairs was achieved using an ultrasonic processor on ice. ChIP-Seq