design: The libraries were constructed using the Illumina Stranded mRNA Prep Ligation Kit (Illumina, USA) from 1 g total RNA per sample. Poly(A)+ mRNA was enriched with oligo(dT) beads, fragmented, and used for cDNA synthesis. Double-stranded cDNA underwent end-repair, adapter ligation, size selection (300-400 bp), and 10-15 cycles of PCR amplification. Final libraries were quantified (Thermo, USA) and sequenced on an Illumina Novaseq X platform by paired-end 163bp