library_construction_protocol: When the intervention was completed, fresh subcutaneous tumors were stored in MACS tissue storage solution (#130100008) and cell suspensions were prepared by tissue homogenate.  The single-cell suspension was added to the single-cell sorting honeycomb plate and excessive magnetic beads with barcodes were put into the plate. During the phase of cell lysis, the RNA was labeled as bounding to the barcode.  Subsequently, reverse transcription of RNA and cDNA synthesis were carried out to obtain cDNA library for subsequent sequencing.  Paired-end sequencing was conducted in the Nova seq6000 sequencing platform to get the transcriptome results.  BD Rhapsody Analysis pipeline was applied to identify the UMI sequence and the cell tag sequence alignment to the reference genome (GRCm39).