library_construction_protocol: In brief, 0.3 g 96 HAI cotyledons from Mock, pER8-LEC2, pER8-LEC2 suvh1suvh3, and pER8-LEC2 ahl15 with DMSO or estradiol treatment were used for nucleus extraction. The extracted nuclei were used for library preparation using TruePrep DNA Library Prep Kit V2 (Vazyme, TD50102) and TruePrep Index Kit V2 for Illumina (Vazyme, TD202) and then sent to Beijing Biomarker Technologies (China) for deep sequencing.