library_construction_protocol: RNA was extracted using Qiagen RNA extraction kit Libraries were prepared using the NEBNEXT Ultra II Directional RNA Library Prep kit (NEB) and the Poly-A mRNA magnetic isolation module (NEB) according to the provided protocol. Poly-A containing mRNA molecules were purified, fragmented and reverse transcribed into cDNA and adapters were ligated to the fragments. Purified, adapter-ligated DNA was then multiplexed and sequenced on NextSeq 2000 platform (Illumina Inc) using the NextSeq 2000 P3 XLEAP-SBS Reagent Kit (100 cycles) to yield ~60 million 50 bp high quality strand-specific paired reads per sample.