design: DNA was extracted from fecal pellet using a stool DNA purification kit (Hi Media, India).  DNA quality was checked by 1 % agarose gel electrophoresis and quantified on Epoch Biotech plate reader. Next generation sequencing was carried out at Biokart, Bangalore, India. In summary, TAQ Master MIX and modified 16S F5 AGAGTTTGATGMTGGCTCAG3 and 16SR 5 TTACCGCGGCMGCSGGCAC3 were used to amplify hypervariable portions of the bacterial 16S rRNA gene. The amplicons of each sample were purified with Ampure beads to remove unused primers, and an additional 8 cycles of PCR was performed using Illumina barcoded adapters to prepare the sequencing libraries. Libraries were purified using Ampure beads and quantified using Qubit dsDNA High Sensitivity assay kit. Sequencing was performed using Illumina Miseq with 2x300PE v3-v4 sequencing kit. The NCBI database used for the analysis of 16S V3-V4 region. The bcl data received from the sequencer is de-multiplexed into raw fastq data.  The de-multiplexed data quality was checked using Fastqc (Version 0.11.9) and Multiqc (Version 1.10.1) tools.