library_construction_protocol: Total RNA was extracted from cells using TRIzol reagent according to the manufacturer's instructions. RNA quality and integrity were assessed using agarose gel electrophoresis and an Agilent 2100 Bioanalyzer. RNA-seq libraries were constructed using a poly(A) mRNA enrichment method. mRNA was fragmented and reverse transcribed into cDNA, followed by end repair, adaptor ligation, and PCR amplification to generate sequencing libraries.