library_construction_protocol: RNA-seq Extract Protocol:_x000B_1.5 ml of meiotic culture was collected per sample, and total RNA was extracted using the RNeasy Mini Kit (Qiagen), following the manufacturer's protocol for mechanical extraction. RNA quality was evaluated using the Tapestation 2200. RNA-seq Library Protocol:_x000B_Library preparation began with 1.2 µg of total RNA. PolyA enrichment was performed using Sera-Mag oligo(dT) Magnetic particles (Sigma-Aldrich), and cDNA synthesis followed the method described in Parkhomchuk et al., 2009. Libraries were prepared using Illumina TruSeq adapters, quantified by qPCR, and sequenced on Illumina NextSeq 500 instruments at the NYU Genome Center.