library_construction_protocol: Deparaffinization: Perform 2 rounds of xylene treatment to remove paraffin, followed by washes in 100% ethanol.  Drying & Proteinase K digestion: Dry the pellet, then incubate with digestion buffer containing Proteinase K at 37–56°C for 15 min to 3 h.  RNA isolation: Use a Qiagen RNeasy FFPE Kit to purify RNA.  DNase treatment was performed to remove residual genomic DNA.  Quantification and QC: RNA concentration was measured using NanoDrop, and RNA integrity was assessed using a Bioanalyzer. RNA libraries for RNA-seq were prepared using SMART-Seq Stranded Kit (Clontech, 634442), MGIEasy Universal Library Conversion Kit (App-A) following manufacturer's protocols.