library_construction_protocol: Total RNA was isolated, and RNA concentration was measured using a NanoDrop 1000. RNA integrity was assessed using an Agilent 2100 BioAnalyzer, and all samples used for RNA-seq had RIN values between 8 and 10. Prior to library preparation, all RNA samples underwent DNase treatment. Sequencing was performed on an Illumina HiSeq 2500 platform using the V4 chemistry to generate single-end reads. Each experimental group consisted of three biological replicates, with matched replicates in the corresponding control group.