library_construction_protocol: Briefly, freshly harvested cells (1×10⁶) were bound to CoA beads 4FF (Smart Lifesciences), resuspended in antibody binding buffer, and incubated overnight with primary antibodies. CUT&RUN experiments with leukemia cells were performed using ex vivo-isolated AML cells from leukemic mice. The samples were then washed and bound to homemade pA/G-MNase. Chromatin digestion was initiated by the addition of CaCl₂ and stopped after 30 minutes with STOP buffer containing chelating agents. Samples were incubated for 30 minutes at 37°C to release CUT&RUN fragments and then treated for 1 hour at 50°C with proteinase K. A purification step was performed using SRPI beads. Library preparation was done using the NEBNext Ultra II DNA Library Prep Kit for Illumina, followed by sequencing on a NovaSeq 6000 to achieve a depth of at least 10 million paired-end 150 bp reads per sample.