library_construction_protocol: Cells were submerged in NaOH (0.2 N) and heated to 70 °C for 20 min in a Thermocycler Life Eco (Falc). After rough pipetting to ensure lysis, 50 μL of Tris HCl (0.04 M). The extracted DNA was used as a template for amplification by PCR with specific primers flanking the Mr-cofilin gene. PCR products were sent to the Technion–Israel Institute of Technology (Israel) for Next-generation sequencing. (Miseq Run V2; 2 × 150 bp, assuming 4 M reads per ends per run)