design: For the purpose of long-read sequencing, a SMRTbell express template prep kit 2.0 was utilized to construct a high-fidelity (HiFi) library from high-molecular-weight (HMW) genomic DNA. Initially, the HMW DNA was carefully handled to prevent shearing and preserve fragment length. The DNA underwent enzymatic treatment for end-repair and damage correction, followed by A-tailing to prepare the fragments for adapter ligation. Subsequently, oversized hairpin SMRTbell adapters were ligated to both ends of the DNA fragments, creating the characteristic circular SMRTbell templates. To enrich for successfully ligated, long-insert templates, the library was subjected to a size-selection step using a BluePippin system, targeting fragments greater than 15 kb. Finally, a cleanup procedure with AMPure PB beads was performed, and the library was treated with an exonuclease to remove any remaining linear or damaged DNA molecules. The resulting purified, size-selected SMRTbell library was quantified and prepared for sequencing on the PacBio Sequel IIe platform.