library_construction_protocol: Frozen tissue (0.5-3mg) previously stored in RNALater ICE (Invitrogen) was homogenized in 1ml of buffer RLT+βME (Qiagen) using a Bead Mill 4 (Fisherbrand) for three cycles of 30s at a speed of 4m/s with 2min ice rests interspersed between cycles.  Total RNA was isolated using the RNeasy Plus (Qiagen) extraction kit with the protocol adjusted proportionally for an input of 825µL of homogenized solution. RNA quality (RIN) and quantity was assessed on the Bioanalyzer 2100 instrument using the RNA 6000 Pico Kit (Agilent). mRNA-seq libraries were prepared using a target input of 100ng of intact (RIN>7) RNA with the KAPA mRNA HyperPrep kit implementing a fragmentation step of 94C for 6min and 15 cycles of final amplification (Roche). Library conversion and deep sequencing were performed using the App-A Universal Library Conversion kit and App-D Primer kit on a DNBSEQ-G400C sequencer with the FCL PE100 sequencing set (Complete Genomics).