library_construction_protocol: Total RNA was extracted using a Qiagen RNeasy Mini Kit with on-column DNase digestion according to the manufacturer's instructions. mRNA was purified from total RNA via polyA enrichment using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis. Sequencing libraries were generated using NEBNext UltraRNA Library Prep Kit for Illumina (NEB) following the manufacturer's recommendation.