library_construction_protocol: Tumor samples were harvest and dissociated into single-cell suspension using Liberase (Sigma) and DNase I (Sigma). The CD45+ tumor-infiltrating lymphocytes were then isolated from the single-cell mixture by using the EasySep™ Mouse CD45 Positive Selection Kit (Stem cell technologies) and resuspended in sterile PBS. The CD45+ TILs were sent to HKU CPOS for further processing. Fresh single-cell suspensions prepared in the previous step were processed using the Chromium Single Cell 5' Library + TCR/BCR Library (10× Genomics) to generate uniquely barcoded RNA, which was then reverse-transcribed into cDNA, PCR-amplified, and used to construct sequencing libraries. The uniquely barcoded cDNA was sequenced on the Illumina NovaSeq 6000 platform. Quality control was performed with Cell Ranger, and the sequencing data were aligned to the mouse genome (mm39), resulting in Cell Ranger output files.