design: ATAC-seq samples were generated from ~5000 sorted cells. Nuclei were isolated suing the OMNI-ATAC protocol, Corces et al. PMID: 28846090. Nuclei were incubated as directed for tagmentation of DNA at 36 degrees for ~40 minutes. Libraries were prepared using Nextera DNA assay and Nextera Index kits. Libraries were cleaned with either a Zymogen clean and concentrate kit or using Ampure XP beads or both befoe they were measure, normalized, and sequenced.