library_construction_protocol: According to the public pathogen databases, in-house tNGS panel was designed to detect 273 pathogens causing infections in different systems based on multiplex PCR and mNGS. The 273 pathogens included 113 bacteria, 47 fungi, 101 viruses, and 12 parasites. After nucleic acid extraction, multiplex PCR with the designed primers was used to construct libraries. Library concentrations were quantified using Qubit 4.60. Illumina NextSeq platform was used for high-throughput sequencing. submitted_instrument_model: Illumina Nextseq 500