library_construction_protocol: Lung tissue was perfused with sterile fetal bovine serum and phosphate-buffered saline instilled in the pulmonary arteries. The right lower lobe was minced and digested into a single cell suspension. Mononuclear cells were isolated and cryopreserved. To obtain leukocytes from the spleen, tissue was minced into small pieces, filtered, and then broken up to release the cells from the outer layer of connective tissue. Mononuclear cells were enriched for leukocytes. They were isolated and incubated with DNase for 10 minutes followed by processes that removed dead cells and debris. Processed leukocytes were further sorted to enrich for lymphocytic cells. Lymphocytic cells were processed for single cell Multiome sequencing on a Chromium X system with a Chromium Next GEM Chip from 10XGenomics. Gene expression and ATAC libraries were constructed with Chromium Next GEM Single Cell Multiome ATAC and Gene Expression Reagent from 10XGenomics.