library_construction_protocol: Tissues were mechanically dissociated a Dounce pestle. Nuclei was pelleted and resuspended in resuspension buffer and centrifuged at 900g for another 15 minutes to pellet the nuclei. Supernatant was removed and nuclei were then stained by adding 1 mL of resuspension buffer containing 0.1 µg mL−1 of DAPI. Afterwards, nuclei were filtered through a 40 μm cell strainer into FACS tubes immediately prior to FACS sorting using a Sony MA900 sorter with a 70 µm chip. Single nuclei were sorted into chilled PCR tubes containing 10 µL of resuspension buffer. Nuclei were verified under microscope where counts were verified using a Fuchs-Rosenthal counting chamber. Libraries were constructed using 10X Chromium Next GEM Single Cell 3′ HT Kits v.3.1.