library_construction_protocol: Total RNA was extracted using a NucleoSpin RNA kit, 2ug of total RNA was used for the construction of seq libraries Integrity of RNA samples was determined using Qubit RNA IQ Assay (Invitrogen). A purification of 2ug from the obtained mRNA was performed using TruSeq RNA sample prep kit v2 (Illumina). Adaptor ligation, purification of 100 base-pair cDNA fragments, amplification of these fragments, and library preparation were performed according to the manufacturer's instructions (Illumina) and sequenced