library_construction_protocol: RNA for RNA-sequencing (RNA-seq) was isolated using RNeasy Plus Kits according to the manufacturer's instructions. After extraction, RNA quality and quantity were determined by Tapestation 4200 (Agilent) with High Sensitivity RNA ScreenTape Assay (Agilent) and Qubit fluorometer with Qubit RNA HS Assay Kit respectively. The RNA integrity number (RIN) for all RNA-seq samples was greater than 8 RNA-seq libraries were prepared using KAPA RNA HyperPrep Kits with Ribo Erase (Roche). Total RNA input for each library preparation was 500 ng quantified by Qubit, and PCR amplification was set with 8 cycles. The quality control of each RNA-seq library was tested by Qubit fluorometer and Tapestation 4200 before pooling. Nine libraries with different KAPA dual-indexed adaptors were multiplexed as one pool and sequenced on one lane of the Illumina HiSeq 4000 sequencing platform (Illumina) at the MDACC Advanced Technology Genomics Core (ATGC) with 5% PhiX spike-in and read format of 76-8-8-76, paired-end.