library_construction_protocol: iMGLs were dissociated with 0.25% Trypsin/EDTA, and FACS was performed to isolate BFP+ cells among the top 50% mCherry-expressing cells.  Cells were spun, washed and counted in FACS buffer lacking EDTA, and the cell suspension containing ~1700 total cells/uL and ~1400 live cells/uL was then subjected to library preparation Libraries were prepared according to the 10X 5' GEM-X scRNA-seq library prep kit with Feature Barcode Kit containing the CRISPR RT oligo.  29,000 cells were loaded onto each of 7 chip wells, and cDNA and sgRNA libraries from each sample were combined into separate cDNA and sgRNA pools. cDNA and sgRNA pooled samples were loaded across four NovaSeq S4 lanes at a 9:1 molar ratio, respectively, and sequenced with 150bp paired end reads