library_construction_protocol: Total RNA was isolated from whole brain and a piece of liver tissue using Qiagen's RNeasy Plus Mini Kit (Hilden, Germany) following the manufacturer's instructions. RNA concentration and purity were measured by spectrophotometry (Nanodrop ND-1000, Thermo Fisher Scientific, Waltham, MA, USA) and integrity was verified using the RNA 6000 Nano Chip on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Ratios of 260/280 and 260/230 were above 2.0 and 2.1, respectively. Median RIN (RNA integrity number) values were 9.1 (brain) and 9.6 (liver). Samples were diluted with RNase-free water to 100 ng/mL (brain) and 125 ng/mL (liver), then treated with DNAse I (amplification grade, Invitrogen, Carlsbad, CA, USA). RNA libraries were prepared from the DNAse I treated total RNA extracted from liver samples. One μg of total RNA was used for library preparation (TruSeq™ RNA Sample Prep Kit v2; Illumina, San Diego, USA) following the manufacturer's instructions.