library_construction_protocol: Cells were dissociated using Trypsin-EDTA, filtered through a cell strainer and resuspended in BD Rhapsody Sample Buffer Libraries were generated on a BD Rhapsody Express machine. Libraries for unmodified samples (unmod) were generated following manufacturers recommendations for the WTA assay. The protocol for v1 BD Rhapsody was used (i.e. a single RPE reaction) and applied to enhanced beads, either with prior RoCKseq modification (rockroi condition) or without modification (unmod). Briefly, the protocol for the modified sample differs from the standard WTA protocol due to the following points: 1) addtion of N1 primer to the RPE PCR (for unmod_roi, rock and rockroi conditions); 2) addition of ROIseq primers to the pool of randomeres during RPE extension (for unmod_roi and rockroi conditions); 3) separate indexing reaction for the samples in which the N1 primer is added; 4) custom sequencing primer