library_construction_protocol: At D5, EDLN were proceeded for cell sorting. Migratory non-LC-non-cDC1 (nLCncDC1, gated as B220-MHCIIhiCD11clowtointXCR1-CD326-) were FACS-sorted in 4 populations accord-ing to the expression of reporters: BFP+RFP+ (B+R+), BFP-RFP+ (B-R+), BFP+RFP- (B+R-) or BFP-RFP- (B-R-). These cells were then labelled with A0301 Hashtag1 (H1) (Biolegend, #155801), A0302 Hashtag2 (H2) (Biolegend, #155803), A0303 Hashtag3 (H3) (Biolegend, #155805) and A0304 Hashtag4 (H4) (Biolegend, #155807), respectively. Meanwhile, cells were also labelled with for Antibody Derived Tags (ADT): TotalSeq™ A0014 anti-CD11b (Biolegend, #101265),  A0002 anti-CD8a (Biolegend, #100773), TotalSeq™ C0422 anti-CD172a(Biolegend, #144033) and TotalSeq™ B0566 anti-CD301b (Biolegend, #146817) The 4 labelled migDC populations were pooled at the ratio 1:1:2:1 (5200 B+R+ cells, 5200 B-R+ cells, 10400 B+R- cells and 5200 B-R- cells), and submitted for 3'mRNAseq library preparation using the Single Cell 3' Reagent Kit v3.1 chemistry (dual index) following the guidelines of the 10X Genomics user manual: CG000317 Rev D, and the Biolegend protocol TotalSeq™-A An-tibodies and Cell Hashing. Single cell suspensions were processed on the 10X Genomics Chromium iX. cDNA amplification was carried out using 11 cycles of PCR. Dual index library construction was performed with 13 (HTO), 14 cycles (ADT), or 12 cycles (3' gene expression library) during sample index PCR. Final library quantification and quality control were complet-ed using Bioanalyzer 2100 (Agilent Technologies, Les Ulis, France). Libraries were sequenced in one lane on an Illumina NextSeq 2000 sequencer as paired-end 28 + 85 base reads.