library_construction_protocol: RNA was prepared using a Direct-Zol RNA prep kit (Zymogenetics) and eluted into water. RNA was pooled from 2 male and 2 female size-matched fish for each replicate with replicates spanning two independent swimming cohorts. Libraries were prepared with 1 ug of input RNA using a TruSeq library prep kit, indexed and pooled, and sequenced using an Illumina NovaSeq 6000 to a read depth of approximately 25 million reads per library.