library_construction_protocol: Cell pellets were processed for MNase digestion as described in (McKnight et al 2021). Briefly, cells were fixed with 1% formaldehyde for 15 minutes and quenched with glycine. Cells were spheroplasted using Zymolyase 100T until >90% of the cells were spheroplasts as assessed by microscopy. Micrococcal nuclease and Exonucelase III were added to each sample and the reactions were incubated at 37°C for exactly 15 minutes. Digestion was halted by the addition of EDTA and EGTA. Each sample was mixed mith S. pombe mononucleosomal DNA (strain FWP570) at a ratio of 1 ng S. pobe DNA / 1.5 x 10^7 S. cerevisiae cells. Mononucleosomal DNA was isolated and used to construct libraries. Libraries were constructed using the NEBNext Ultra II DNA library Prep Kit for Illumina (New England Biolabs) following manufacturer specifications.