design: First, extract the total RNA from the samples using the Trizol method, and perform quality control of the samples using the Thermo NanoDrop One and Agilent 4200 Tape Station. After passing the quality check, remove ribosomal RNA using the Epicentre Ribo-Zero rRNA Removal Kit, and construct a strand-specific library using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina.