design: RNA-Seq of Xenopus laevis Retina after Crispr-mediated Rhodopsin depletion
library_construction_protocol: Tadpoles were euthanized in 0.05% Benzocaine (Sigma), then transferred into a petri dish containing 1X HBSS (Sigma). The eyes were surgically dissected out with sharp forceps under the stereo microscope. For RNA extraction, the lens and retinal pigment epithelium were carefully removed, leaving the retinal tissue intact. The tissue was collected and kept in -80°C until use.  Total RNA was isolated from the dissected retina of the Xenopus tadpoles using TRIZOL (Invitrogen) according to the manufacturer's protocol, followed by RNA purification with the NucleoSpin RNA mini kit (Machere-Nagel, Germany) OligodT selected mRNAs followed by Illumina mRNA stranded library preparation