design: Nanopore sequencing was carried out on a MinION sequencer (Oxford Nanopore Technology, Oxford, UK) in 72 h single runs, using FLO-MIN106D flow cells and PCR-cDNA sequencing-barcoding (SQK-PCB109, ONT, Oxford, UK). The sequencing libraries were prepared in accordance with the manufacturers protocol and sequenced immediately. Outputs varied between 300 and 902 Mb.