library_construction_protocol: RNA was extracted using Qiagen RNAeasy Plus mini kit. 200 ng was used for the construction of sequencing libraries. Novogene performed quality control on all RNA samples. RNA libraries were constructed and sequenced using the standard mRNA library preparation (poly A enrichment) protocol with paired-end sequencing (150 bp) by Novogene Corporation Inc. (Sacramento CA). This included messenger RNA purification from total RNA using poly-T oligo-attached magnetic beads, followed by fragmentation, cDNA synthesis, end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was quality checked using Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection. Quantified libraries were pooled and sequenced on Illumina NovaSeq X Plus.