library_construction_protocol: ST on FFPE samples was perfomed using the 10X Genomics Visium Spatial Gene expression for FFPE protocol V1, using the human whole transcriptome probe set. Tissue areas up to 6.5 x 6.5 mm were pre-selected by a pathologist following H&E staining. 5 µm sections of the selected areas were sectioned, dehydrated and deposited on the Visium Gene Expression V1 chip as recommended by the manufacturer. Spatial transcriptome sequencing libraries were prepared with 10xGenomics Visium Spatial Gene Expression for FFPE Slides & Reagent kit (GC000407) according to manufacturer's instructions. Captured sequences were hybridized with the human whole transcriptome probe kit (PN1000364 or PN1000363). Prepared libraries were sequenced on the NovaSeq 6000 system (Illumina) using S4 Flow Cells with a target minimal reads per covered spot of 50k.