design: RNA-seq of isolated primary human CD8+ T cells treated with KDACi with polyclonal stimulation for 6 days
library_construction_protocol: CD8+ T cells were isolated by positive magnetic selection from leucocyte cones from healthy blood donors before being stimulated with anti-CD2/3/28 microbeads in complete RPMI medium with non-limiting IL2 (10ng/ml) in the presence of either sodium valproate (500uM), vehicle (1 in 10,000 DMSO) or sodium valproate (175uM) with concurrent glutaminolysis inhibition (R162, 25uM) Total RNA was extracted from harvested cell populations using an RNeasy mini kit (Qiagen) with quality assessed using an Agilent BioAnalyser 2100 and RNA quantification performed using a NanoDrop ND-1000 spectrophotometer. Library preparation was undertaken using the SMARTer Stranded Total RNA-seq v2 Pico Input Mammalian kit according to the manufacturer's instructions