library_construction_protocol: At D5, BFP+ and BFP- migDC (B220- CD11cint MHCIIhi) from NT and MC903-treated EDLN were enriched by FACS. NT/BFP+ (5000 cells) and NT/BFP- (7000 cells) migDCs cells were then labelled with anti-bodies (anti-CD45/anti-MHC-I) tagged with hashtag oligonucleotide (HTO), TotalSeq™ barcoded antibody A0301 Hashtag1 (H1)and A0302 Hashtag2 (H2) respectively. MC903/BFP+ (5000 cells) and MC903/BFP- (7000 cells) migDCs were labeled with A0303 Hashtag3 (H3) and A0304 Hashtag4 (H4), respectively. NT cells (H1 and H2 labelled cells) and MC903 cells (H3 and H4 labelled cells) were then submitted respectively to 3'mRNAseq library preparation using Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3 (16 rxns PN-1000075) following the guidelines of 10X Genomics User Manual "CG000185 RevB", and the Biolegend protocol TotalSeq™-A Anti-bodies and Cell Hashing. Single cell suspensions were processed on the 10X Genomics Chromium Controller. Amplification of cDNA was carried out using 11 cycles of PCR. Construction of single index HTO and 3' gene expression libraries was performed with 12 cycles of amplification during sample index PCR. Final libraries quantification and quality control were completed using Bioanalyzer 2100 (Agilent Technologies, Les Ulis, France) before sequencing using the Illumina HiSeq 4000 platform as paired-end 2x100 base reads (NT and MC903 cells were sequenced in two different lanes).