library_construction_protocol: Dissected brain tissue was homogenized in Trizol (Thermo Fisher) to protect against RNA degradation. Chloroform (50ul) was then added, and the tissue and shaken for 20 seconds before a 15-minute incubation at room temperature. Samples were centrifuged (14000rpm, 15 minutes at 4°C) and the aqueous phase was transferred to 70% ethanol. RNA extraction was performed using the Qiagen RNasy minikit (cat #74108 Thermo Fisher), while DNA was synthesized using the qScript cDNA Synthesis Kit (cat#101414-100, Quanta Bioscience). Qualifying samples were then prepped following the standard protocol for the NEBnext Ultra ii Stranded mRNA (New England Biolabs).