design: This sample represents NE492, a transposon insertion mutant of Staphylococcus aureus strain JE2 from the Nebraska transposon mutant library. The mutant is intended to carry a disruption in the SAUSA300_1793 gene. Whole genome sequencing was performed using Illumina paired-end technology to confirm the transposon insertion site. This identified a transposon disruption in the oppF gene, rather than SAUSA300_1793.