design: Low Input Micro-C (Pico-C) data of Drosophila melanogaster embryos across early nuclear cycles
library_construction_protocol: For ATAC-seq on Jabba controls and knockdowns, single Drosophila embryos were collected, staged (nuclear cycle 14), and dechorionated using 50% bleach. Individual embryos were transferred into chilled lysis buffer, manually homogenized under a dissecting microscope, and nuclei were pelleted by centrifugation.  Transposition was carried out using 2.5 µL Tn5 transposase in a 10 µL reaction volume at 37°C for 30 minutes. Transposed DNA was purified using a MinElute cleanup kit (Qiagen) and amplified by PCR with indexed primers. Final libraries were cleaned using SPRI bead purification (1.2× ratio) and quantified by Qubit prior to sequencing.