library_construction_protocol: Total RNA was extracted from GFP and pORF3 OE HeLa cells using the RNA Isolation Kit (Yeasen), following the manufacturer's protocol. 10 ng of total RNA was then subjected to cDNA synthesis and amplification using the Smart-seq2 method as described previously (Picelli et al., 2013). Sequencing libraries were constructed from 500 pg of amplified cDNA using TruePrep DNA Library Prep Kit V2 for Illumina (Vazyme) according to the manufacturer's instructions. Libraries were sequenced on the Illumina HiSeq X Ten platform to generate 150 bp paired-end reads.