design: RNA was extracted from cell pellets using Qiagen Rneasy Mini Kit and RNA sequencing library preparation used NEBNext Ultra II RNA Library Preparation Kit for Illumina per manufacturers instructions. Sequencing libraries were validated using the Agilent Tapestation 4200, and quantified using Qubit 4.0 Fluorometer as well as by quantitative PCR. The sequencing libraries were multiplexed and clustered onto a flowcell on the Illumina HiSeq 4000 according to manufacturers instructions. The samples were sequenced using a 2x150bp paired end configuration.