library_construction_protocol: Cells were perforated for primary and secondory antibody binding, and then cut and adaptor ligated by hyperactive PA/PG-Tn5 transposase from Vazyme. DNA was extracted using DNA extraction beads from Vazyme. CUT&Tag-seq libraries were prepared using  extracted DNA as starting material. Libraries were amplified using 15-20 cycles on the thermocycler using indexing system of Vazyme. Post amplification libraries were size selected at 150-450bp in length using DNA extraction beads from Vazyme. Libraries were validated using the Agilent High Sensitivity DNA Kit.