design: CHO-B2 cells were infected with recombinant Ghana virus (rGhV M-GS*) at an MOI of 0.05. Total RNA was harvested at 72 hours post-infection using TRIzol, confirmed non-infectious, and prepared using the Direct RNA Sequencing Kit (SQK-RNA004, Oxford Nanopore). Libraries were sequenced on a FLO-MIN004RA flow cell.