design: Plasmids from the conjugation transfer process in section 2.3.2 were selected, specifically by picking at least 300 green fluorescent colonies from the resistant plates and transferring them to a 5 mL of PBS solution. Then, 100 L of this solution was inoculated into 10 mL of selective liquid LB medium containing the aforementioned antibiotic combination and incubated overnight. Next, conjugative plasmids were extracted following the protocol of the Mini Plasmid Kit (Tiangen Biotech Co., LTD., China). Metagenomic DNA from the two environmental water samples was extracted according to the protocol of the Bacteria DNA Kit (Tiangen Biotech Co., LTD., China). The concentrations of the extracted DNA was measured using a Qubit 3 fluorometer (Thermo Fisher Scientific Inc., USA). The metagenomic sequencing of the genomic and plasmid DNA was conducted by NovoGene (Shanghai, China) on the Illumina Novaseq platform with a PE150 strategy.  The plasmid samples were obtained from the conjugates generated during the conjugation transfer experiment described in section 2.3.2. We selected green fluorescent colonies from the resistant plates of the Dashahe_CK, Dashahe_PVC/5, Receiving Water_CK, and Receiving Water_PVC/5 experimental groups. These were named as Dashahe, Dashahe with Plastic Leachate, Receiving Water, and Receiving Water with Plastic Leachate, with each experimental group set up with three replicates, totaling 12 plasmidomes. The plasmids from each plasmidome were individually extracted and sequenced independently, with no pooling of samples.