design: Ribosomal RNA (rRNA) depletion instead of poly(A) purification is performed by RiboCop rRNA Depletion Kit for Mixed Bacterial SampleslexogenUSAand then all mRNAs were broken into short (200nt) fragments by adding fragmentation buffer firstly. Secondly double-stranded cDNA was synthesized with random hexamer primers (Illumina). When the second strand cDNA was synthesized, dUTP was incorporated in place of dTTP. Then the synthesized cDNA was subjected to end-repair, phosphorylation and A base addition according to Illuminas library construction protocol. RNA-seq transcriptome library was prepared following Illumina Stranded mRNA Prep, Ligation (San Diego, CA) using of total RNA. paired-end RNA-seq library was sequenced with the Illumina Novaseq Xplus(or other new sequenator) (Illumina Inc., San Diego, CA, USA) .The processing of original images to sequences, base-calling, and quality value calculations. The clean reads by removing low-quality sequences, reads with more than 10% of N bases (unknown bases) and reads containing adaptor sequences.