design: DNA extraction was carried out following the manufacturers practice provided by QIAGEN QIAamp DNA Mini Kit (Ref. 51304, QIAGEN, 40724 Hilden, Germany). DNA was quantified using both the Eppendorf BioPhotometer D30 and the fluorimeter Qubit dsDNA BR Assay Kit (Ref. 32850, Invitrogen, 92008 Carlsbad, CA, USA).A concentration of 10 ng of each sample was used for NGS sequencing via Illumina MiSeq platform according to the manufacturers instructions provided in QIAseq FX DNA Library Core Kit (Ref. 1120146, QIAGEN, Hilden, Germany). Libraries were quantified and their quality evaluated using both the fluorometric Qubit dsDNA HS Assay Kit (Ref. Q32851, Invitrogen, Carlsbad, CA 92008, USA) and the Agilent High Sensitivity DNA Kit (Ref. 5067-4626). Denature and dilute libraries were performed following the Denature and Dilute Libraries Guide protocol provided by Illumina, choosing 8,5 pM as the loading concentration. Finally, sequencing was performed using the MiSeq Reagent Kits v3 (Ref. 15043895, Illumina, Inc., 92122, San Diego, CA, USA). The Sample Sheet was created using the Local Run Manager v3 software and following the instructions in the Local Run Manager v3 Software Guide provided by Illumina.