library_construction_protocol: Cells were washed, trypsinized, centrifuged, and frozen at -80 °C for later RNA extraction. RNA was extracted using the NucleoSpin RNA XS kit (Macherey-Nagel, 740902) and residual DNA was removed using RNase-Free DNase from the NucleoSpin Mini RNA kit (Macherey-Nagel, 740955). RNA quantity and quality were assessed using a NanoDrop® 2000c spectrophotometer (Thermo Fisher Scientific) Unstranded libraries were prepared by following the Smart-seq 2 protocol until the PCR amplification step and continued with the Nextera XT DNA library prep kit (Illumina, FC-131-1096) using the MGI universal and index primers for PCR amplification.