library_construction_protocol: Total RNA was extracted from WT and Connexin 43 knockout quiescent (0h) and dividing (48h) HSC (Lin- /cKit+/Sca1+/CD48-/CD150+) using RNeasy Mini Kit (QIAGEN) according to manufacturer's protocols. RNA quality and concentration were measured by Bioanalyzer 2100 using the RNA 6000 Nano Assay (Agilent Technologies). RNA-seq libraries were prepared using the Illumina TruSeq RNA preparation kit and sequenced on the Illumina HiSeq 2500 following the manufacturer's protocol. The concentration of the pool was optimized to acquire at least 25-30 million reads per sample using Paired-End Reads with a read length of 75 bps.