design: Comparison of epithelial (EpCAM+) and non-epithelial (EpCAM-) intestinal populations derived from induced pluripotent stem cells using a novel protocol
library_construction_protocol: Cells were washed with PBS 1x and then lysed with the lysis buffer (see extraction kit details) RNA was extracted using GenElute™ Mammalian Total RNA Miniprep Kit following manufacturer's instructions (Cat no. RTN70 - Sigma Aldrich) PolyA enrichment of mRNA, RT first-strand cDNA library with ligation of sequencing adapters