library_construction_protocol: RNA extraction was performed as described in Ingolia, N. T., Brar, G. A., Rouskin, S., McGeachy, A. M. & Weissman, J. S. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc 7, 1534–1550 (2012) For Ribo-seq, Library construction was performed as described in Ingolia, N. T., Brar, G. A., Rouskin, S., McGeachy, A. M. & Weissman, J. S. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat Protoc 7, 1534–1550 (2012). For RNA-seq, RNA was used to purify mRNA using Takara Oligotex-dT30 kit according to manufac-turer's instructions. Purified RNA was fragmented using alkaline fragmentation buffer containing 27 mM Na2CO3 , 72 mM NaHCO3 , 2 mM EDTA, at 95 ℃ for 40 min. Fragments corresponding the length of 25 to 34 nt were used for the library construction using protocol describe above.