library_construction_protocol: Sorted Δ42PD-1+, PD-1+ and double negative B cells at 48 hours after the treatment with α-IgM/G F(ab')2 and CH34 or isotype control IgG2b. Isolated Δ42PD-1+, PD-1+ T and Δ42PD-1-PD-1- T cells were labeled with sample tag and Ab tag before loaded into BD Phapsody for RNA exraction, RNA was extracted according to BD Rhapsody protocol library was prepared according to BD Rhapsody protocol single-cell RNA-seq