library_construction_protocol: 10X Chromium to capture enough single cell according to the 10XGenomics Chromium Single-Cell 5' kit (V3) manufacturer's instructions. The reverse transcription, cDNA amplification and library construction steps were performed according to the standard protocol (10X Genomics). Sequencing libraries were sequenced on an Illumina NovaSeq 6000 sequencing system (paired-end multiplexing run,150bp) by LC-Bio Technology co.ltd (HangZhou, China) at a minimum depth of 20,000 reads per cell. submitted_library_strategy: ssRNA-Seq