library_construction_protocol: Cells grown in 2D collected following trypsinisation, and cells grown in 3D collected after incubation with 2.25μg/mL collagenase D (Sigma) at 37C for 1hr. Collagenase activity was subsequently inhibited by HBSS addition (Thermofisher). Library construction was performed as per manufacturers instructions, using the chromium 10X 3' kit, v2.