library_construction_protocol: Total RNA was purified from Tlr7Y264H primary BMDMs using the PureLink RNA Mini Kit (Thermo Fisher Scientific) and DNasetreated using the Purelink DNASE set (Thermo Fisher Scientific). 25 ng of total RNA from each sample was used for the construction of sequencing libraries. Libraries were generated using an in-house multiplex RNA-seq method (version 01/09/2021; Hudson Genomics Facility). 25 ng of total RNA from each sample was tagged with an 8 bp sample index and 10 bp unique molecular identifier (UMI) during initial poly(A) priming with the addition of a template-switching oligo. After cDNA amplification Illumina P5 adaptors with distinct i5 indexes were added by tagmentation by Nextera transposase and PCR. Final libraries were pooled based upon size-adjusted qPCR and single-end sequencing was performed on a NextSeq 2000 run using a P3 50 cycle kit (cDNA reads generated 61 nt).