library_construction_protocol: Biopsies were taken from anhidrotic skin areas before corticosteroid therapy and from adjacent sweat-recovered areas after therapy. For each skin sample, a 5 mm punch biopsy was obtained after local anesthesia and was placed immediately into 1 mL BAMBANKER freezing medium (Nippon Genetic Co.) on ice and stored at –80°C until analysis. Cryopreserved human skin tissues were thawed in a 37°C water bath and cut into small pieces using scissors. The tissues were then transferred to 0.5 mL of 0.25% Trypsin-EDTA (Gibco, Life Technologies) and incubated at 37°C for 20 minutes. After enzymatic digestion, the tissues were transferred to the dissociation solution provided in the Miltenyi Human Whole Skin Dissociation Kit (Miltenyi Biotec) and incubated for 2.5 hours, following the manufacturer's instructions. Subsequently, the partially dissociated tissue was manually disaggregated by pipetting up and down 10 times through an 18G needle. The resulting cell suspension was filtered through a 70 μm cell strainer (Corning, 352340) and kept on ice. Cells were pelleted by centrifugation at 300 × g for 10 minutes and resuspended in 1 mL of RPMI medium. Viable cells were counted using 0.4% Trypan Blue solution (Gibco, Thermo Fisher Scientific; 15250061). Isolated single-cell suspensions were then subjected to droplet-based single-cell RNA sequencing. Single-cell libraries were formed by using the Chromium Next GEM Single-Cell 5′ Reagent Kit v2 (Dual Index)(10x Genomics) in line with the manufacturer's protocol  (https://www.10xgenomics.com/jp/support/single-cell-immune-profiling/documentation/steps/library-prep/chromium-single-cell-5-reagent-kits-user-guide-v-2-chemistry-dual-index). Sequencing was performed using Illumina NextSeq 2000.