library_construction_protocol: Fly heads were obtained from samples of 50-60 flies through flash freezing with liquid nitrogen, followed by vortexing and separation through a series of a sieves. Fly heads were then suspended in homogenization buffer and nuclei released into solution through chemical and physical agitation of the cell membrane. Anti-GFP bound beads were then used to isolate GFP-positive nuclei from the whole head (WH) homogenate to represent the mushroom body-specific fraction (MB). RNA was then isolated from MB and WH fractions using a PicoPure RNA Isolation Kit (Invitrogen:KIT0204) according to the manufacturer instructions. RNA libraries were generated using the Tecan Universal Plus Total RNA-Seq library preparation kit (Tecan: 3061-A01) according to manufacturer instructions.