library_construction_protocol: RNA was extracted with RNeasy Mini Kit (Qiagen); quality and concentration were assessed using the Agilent 2100 Bioanalyser. PolyA enrichment was performed using NEBNext® Poly(A) mRNA Magnetic Isolation module. Libraries were made using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® according to manufacturer's instructions.