design: The file contains sequences of a multiplex combined from the samples collected from MycoPins (wooden pins) which decayed in transect B part 4, positive control (SynMock, a mix of plasmids with artificially synthesized ITS inserts, description available in Palmer 2018, doi: 10.7717/peerj.4925), and negative control (water). Each PCR product is produced by fITS7-ITS4 primers, the primers were modified to include a unique 10-nucleotide tag to create unique pairs of primers. Later, multiple PCR tagged products were mixed together in a multiplex, which was sent for sequencing to Azenta, USA. The sequencing facility attached the Illumina adaptors, prepared the library and performed high-throughput sequencing using Amplicon service on Illumina MiSeq instrument, 2X250 paired-end configuration. In this file there is a mix of PCR products with different tags. For example, sequences with tag 1 will look like this: "Illumina adaptor"-"tag 1F sequence"-"ITS7 primer sequence"-"ITS2 sequence of a species" - "ITS4 primer sequence reverse complement"-"tag 1R reverse complement sequence"-"Illumina adaptor". The complete primer sequences for the PCR is the following: CACACGCTGT TCCTCCGCTTATTGATATGC, ITS4-tag_1; CACACGATCT GTGARTCATCGAATCTTTG, fITS7-tag_1; CACATGTCGT TCCTCCGCTTATTGATATGC, ITS4-tag_4; CACATAGTCT GTGARTCATCGAATCTTTG, ITS7-tag_4; CACGCAGCAT TCCTCCGCTTATTGATATGC, ITS4-tag_5; CACATGACTT GTGARTCATCGAATCTTTG, ITS7-tag_5; CACTAGCGCT TCCTCCGCTTATTGATATGC, ITS4-tag_6; CACGATCAGT GTGARTCATCGAATCTTTG, ITS7-tag_6; CACTATGCAT TCCTCCGCTTATTGATATGC, ITS4-tag_7; CACGTGCTCT GTGARTCATCGAATCTTTG, ITS7-tag_7; CACTCACACT TCCTCCGCTTATTGATATGC, ITS4-tag_8; CACTATAGCT GTGARTCATCGAATCTTTG, ITS7-tag_8; CACTCTGAGT TCCTCCGCTTATTGATATGC, ITS4-tag_9; CACTATGTGT GTGARTCATCGAATCTTTG, ITS7-tag_9; CACTGATCAT TCCTCCGCTTATTGATATGC, ITS4-tag_10; CACTCAGAGT GTGARTCATCGAATCTTTG, ITS7-tag_10; CACTGTATGT TCCTCCGCTTATTGATATGC, ITS4-tag_11; CACTCTCACT GTGARTCATCGAATCTTTG, ITS7-tag_11; CAGACATAGT TCCTCCGCTTATTGATATGC, ITS4-tag_12; CACTGCTACT GTGARTCATCGAATCTTTG, ITS7-tag_12; CAGACTATGT TCCTCCGCTTATTGATATGC, ITS4-tag_13; CAGACAGTGT GTGARTCATCGAATCTTTG, ITS7-tag_13; CAGAGCTCAT TCCTCCGCTTATTGATATGC, ITS4-tag_14; CAGACATCTT GTGARTCATCGAATCTTTG, ITS7-tag_14. This file contains the following amplicons (number of the sample, tag number); 018607E, 1; 018608A, 4; 018608B, 5; 018608C, 6; 018609C, 12; 018609E, 13; 018609F, 14. Please note that this file also contains some sequences that are from a different experiment unrelated to the publication, please see for the full description: doi 10.3897/BDJ.13.e155520