design: ChIP-seq analysis for H3K27ac in 30 patient-derived tissue samples of primary and metastatic melanoma
library_construction_protocol: Fresh frozen tissue samples were provided by the biobank of Azienda USL-IRCCS of Reggio Emilia. Tissues were homogenized with dounce homogenizer in 1% PBS, then crosslinked for 15 minutes with 1% formaldehyde, lysed and sonicated for 12 cycles (30” ON, 30” OFF) using Bioruptor Pico Sonicator. Chromatin was precipitated overnight using Dynabeads Protein G magnetic beads and 3 µg of anti H3K27ac (Abcam). 1% of total chromatin was used as input.  Library was prepared following NEBNext Ultra II DNA Library Prep Kit for Illumina using 5-20 ng ChIP DNA as starting material.