design: Microbial DNA was extracted using the SKYAmp Stool DNA Kit (SkyGen, Moscow, Russia). Library preparation targeting V3-V4 regions of the 16S rRNA gene was conducted with the Quick- 16S NGS Library Prep Kit (Zymo Research, Irvine, CA, USA) according to the manufacturers protocols. Amplification of the targeted region was done using 341F (CCTACGGGDGGCWGCAG, CCTAYGGGGYGCWGCAG) and 806R (GACTACNVGGGTMTCTAATCC) primers from the abovementioned kit. The pooled library was sequenced on Illumina MiSeq with a v3 Reagent Kit (600 cycles) according to the manufacturers instructions (Illumina, San Diego, CA, USA).