design: Approximately 3-5 million cells were crosslinked in 2% formaldehyde for 10 minutes at room temperature and the reaction was then quenched using a solution glycine + BSA. Samples were then washed in PBS and snap frozen on dry ice and then transferred to -80C for long- term storage. 2 million cells were then used for library preparation following the two-restriction enzyme Arima Hi-C kit  and the KAPA HyperPrep kit  manufacturers protocol using Illumina TruSeq dual indexes. Library size was checked by TapeStation 4200 and QC was done by quantstudio using the NEBNext Library Quant Kit for Illumina . Sequencing was performed on the NovaSeq6000 platform using NovaSeq S4 flow cells at a target depth of 350 million reads per library and generating 150bp paired-end reads.