library_construction_protocol: Total RNA was extracted using column separation by the Qiagen Rneasy Micro Kit. RNA which met RIN >8.5 (Agilent Bioanalyzer) was used for library prep. Ribosomal RNA was depleted (NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat, New England Biolabs, USA), and this rRNA-depleted RNA was used as input for the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (New England BioLabs, USA) in which libraries were tagged with unique adapter-indexes. Final libraries were validated on the Fragment Analyzer, quantified via qPCR, and pooled at equimolar ratios. Pooled libraries were diluted, denatured, and loaded onto the Illumina NovaSeq X sequencing system, following the Illumina User Guide for a paired-end run.