design: The nuclei suspension was loaded on a Chromium Controller (10X Genomics) with targeted nuclei recovery of 5,00010,000 per reaction. 3 gene expression libraries were prepared according to the manufacturers instructions of the v3 Chromium Single Cell Reagent Kits (10X Genomics). Quality control of final library cDNA was done using Bioanalyzer High Sensitivity DNA Analysis (Agilent). Libraries were sequenced on a NovaSeq