design: For each sample the RNA concentration was determined and 20 ng RNA was aliquoted for transcriptomics analysis. As input for mRNA capturing, fragmentation, cDNA synthesis, adapter ligation and library amplification, 20 ng of RNA was used. The bread purified library was normalized and sequencing was performed on the NextSeq2000 system (Illumina Inc. San Diego, CA, USA) with a read setup of 1x100 bp