design: cell line=HEK293T; ATXN2 polyQ expansion cell model; polyQ=32Q; generated using the RecT7-KI system; sgRNA(ATXN2)=GGCTTCAGCGACATGGTGAG; Cells cultured in 96-well plates were lysed in Buffer TCL, RNA was purified with 2.2x VAHTS RNA Clean Beads. cDNA libraries were generated using a modified SMART-seq2 protocol, followed by PCR amplification, Tn5 tagmentation, library PCR, and bead purification.