library_construction_protocol: The MagMax-96 total RNA isolation Kit (Ambion, Austin, TX, USA) was used to extract total RNA from gastrocnemius muscle (10 mg / n>5 per group) according to the manufacturer's protocol. The quantity and quality of extracted RNA were determined using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). RNA Integrity Number (RIN) from Bioanalyzer were >9.0, indicating high quality of RNA isolated from muscle biopsies, and these were subjected to NGS analysis. The TruSeq Stranded Total RNA with Ribo-ZeroTM Human/Mouse/Rat kit was used to make RNA-seq libraries, which were prepared according to the manufacturer's instructions.