library_construction_protocol: Human lung tissue was washed with cold 1X PBS twice in a 10 cm petri dish and placed in digestion media containing dispase (5 U/ml, Corning 354535), Collagnease type I (478 U/ml, Thermo Scientific 17100017), DNase I (10 U/ml, Sigma-Aldrich 10104159001) in DMEM-F12 (Corning MT 10-092-CV). Tissues were digested for 1 hour at 37°C. After digestion, the cell suspension was filtered through 100-µm strainer (Corning 352360). Red blood cells were removed using Red Blood Cell Lysis Buffer. Cells were washed twice with FACS buffer (1% FBS, 1mM EDTA in PBS) and filtered through a 40-μm strainer (Corning 352340), pelleted again (450xg, 5 min, 4 °C), and resuspended in PBS containing Zombie Aqua™ Fixable Viability Kit (BioLegend 423101) for 20 minutes. Cells were washed and resuspend in FACS buffer with Human TruStain FcX (BioLegend 422302) for 10 minutes to block non-specific binding of antibodies RNA-seq libraries were prepared based on the 10X Genomics protocol (CG000315 Rev E) using 10000 nuclei per replicate. Single-cell separation was performed using the Chromium Next GEM Chip G Single Cell Kit. The RNA-seq libraries were sequenced in paired-end mode on an Illumina Novaseq platform with the Novaseq S4 flowcell.