library_construction_protocol: Cells were spun down at 300g at 4°C for 10 minutes, and digestion buffer replaced with 1X HBSS + 10% FBS. Cells were filtered, into a FACS tube and incubated with anti-CD31 BV421 and anti-CD45 per-CP antibodies. CD31+ CD45- endothelial cells in different cell cycle states were sorted using a FACS Melody, collected in lysis buffer and RNA extracted via Qiagen Rneasy Micro kit. Library was constructed via NovoGene™ low input RNA sequencing pipeline using illumina chemistry.