library_construction_protocol: RNA was harvested using Rneasy mini kit (Qiagen). Samples A-B were raised to 24hpf, 48hpf, and 72hpf and 100ng of each timepoint was pooled after mRNA extraction. Samples A-C total RNA was sent to Novagene for QC and library preparation. RNA integrity was checked using Agilent 5400 -  all samples were above RIN 8.0.