design: After the genomic DNA of the sample passes quality testing, the DNA is fragmented using enzymes. Subsequently, the fragmented DNA is purified and used to construct a sequencing library through the following sequential steps: DNA end repair, A-tailing at the 3' end, ligation of sequencing adapters, recovery of target fragments via agarose gel electrophoresis, PCR amplification of the target fragments, culminating in the construction of the sequencing library. Prior to sequencing, a quality check is performed on the prepared library. Upon passing quality control, sequencing is carried out using the Illumina Novaseq platform.