design: Briefly, uninjured mature skin tissue, anterior lateral wounds, and anterior ND-A blastemas were harvest and fixed in 1.5% formaldehyde for 20 minutes. After quenching and cell lysis, chromatin was sonicated using a Branson Ultrasonics Sonifier S-450. After elution, input samples were collected, and immunoprecipitation was achieved by incubating the sample with the anti-H3K27me3 antibody (A4039050, Epigentek) overnight and precipitating with the aid of bovine serum albumin-blocked A/G Plus Agarose beads. All samples were centrifuged (12000 g, 30 sec), and the supernatant was collected. After centrifugation and processing, DNA was purified by a QIAquick PCR Purification Kit. DNA libraries were then prepared and barcoded to facilitate multiplexing using the Kapa DNA Hyper Prep Kit (07962355001, Roche). Samples were then validated using the Agilent 2100 Bioanalyzer system. Sequencing was conducted using the HiSeq 2500 System, rapid Run 1 x 51, per 2 lane FC.