library_construction_protocol: RNA was isolated from JH716-18, JH716-18-LN1A, and JH716-18-LN2A cell lines using the Quick-RNA MicroPrep kit (Zymo Research, R1051). Purified RNA was quantified using QuBit RNA BR Assay (Thermo Fisher, Q10210). Libraries were made using Illumina TruSeq Stranded mRNA kit