design: The Oxford Nanopore Technologies (ONT) library preparation primarily involves the ligation of specialized sequencing adapters to the ends of fragmented or full-length DNA/RNA molecules. After initial end-repair and dA-tailing of the target sequences, these adapterswhich are pre-bound to a motor proteinare enzymatically attached to the template. This motor protein is essential for unwinding the double-stranded DNA and translocating the single strand through the synthetic biological nanopore at a consistent rate. The resulting library is then purified using magnetic beads to remove excess adapters and enzymes, finally being loaded into a flow cell where the molecules are captured by the pores for real-time electrical signal detection.