design: After being qualified for genomic DNA testing. Sequencing library preparation (Oxford NanoporeTechnologie) was performed following the experimental report of the SQK-LSK109 sequencing kit.Libraryquality was detected by Qubit 4.0 Fluorometer (Life Technologies, Grand Island, NY) and Average fragmentsize was estimated on an Agilent 4200 (Agilent, Santa Clara, CA).At last, the library was sequenced on anNanopore MinION.