design: Genomic DNA was isolated from leaf tissue using the Tiangen DP305 Plant Genomic DNA Kit (Beijing, China). Libraries were prepared with the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA; Cat. E7370L) following the manufacturers protocol, with index codes added during preparation. DNA was sonicated to ~350 bp, and fragments were end-repaired, A-tailed, and ligated to Illumina adapters, followed by PCR amplification. PCR products were purified using AMPure XP beads (Beverly, USA). Library quality was assessed on an Agilent 5400 system, and concentrations were quantified by qPCR (1.5 nM). Qualified libraries were pooled and sequenced on Illumina platforms using the PE150 strategy at Novogene (Beijing, China).