library_construction_protocol: The samples were irradiated twice with 254 nm UV-C light on ice, with each irradiation dose of 400 mJ. Subsequently, the samples were lysed with lysis buffer on ice for 15 minutes. The lysate was further treated with 40 U of RNase I and incubated with rotation at 37°C for 5 minutes. libraries were prepared for sequencing using eCLIP-seq library construction protocols