library_construction_protocol: 1 mL of nuclear lysis buffer (10 mM Tris-HCl, pH=7.4; 10 mM NaCl; 3 mM MgCl₂; 0.1% NP40; 0.1% Tween 20; 0.01% Digoxigenin) was added to resuspend the tissues. The samples were then homogenized using a Dounce homogenizer with 10 strokes each using pestle A and pestle B. The nuclear suspension was filtered through a 40 µm Flowmi Cell strainer, followed by centrifugation at 800 × g for 5 min at 4 °C. The resulting pellet was resuspended in wash buffer. Nuclear integrity was assessed by methylene blue staining, and nuclei concentration was determined using a hemocytometer (BS-QT-1103, Biosharp, Anhui, China) under a Nikon Eclipse Ni DIC microscope (model YTV55). CUT&Tag according the Hyperactive Universal CUT&Tag Assay Kit protocol (Vazyme, Nanjing, China).Cell counting was performed by using a Cellometer Mini (Nexcelom Bioscience, Boston, MA, USA). pA-Tn5 transposase was used to cut the genome and add a special adaptor sequence to build a library.