library_construction_protocol: The pre-infusion product CAR-IL-15 iNK were harvested from in vitro expanded culture. The mouse peritoneal tumors were harvested in cold serum-free RPMI-1640 medium and total cells were isolated using tumor dissociation kit (Miltenyi Biotec) according to manufacturer's instructions. Briefly, tumor tissues were digested in serum-free RPMI-1640 medium supplemented with human tumor dissociation kit enzymes (Miltenyi Biotec) at 37°C under continuous rotation for 90 min. Dissociated single cell suspensions were then filtered through a 70 μm mesh filter and pelleted at 300 × g for 5 minutes. The harvested pre-infusion CAR-IL-15 iNK products and the single cells isolated from the tumors were washed with PBS+0.04% BSA. Dead cells and debris were removed using Dead Cell Removal Kit (Miltenyi Biotec, 130-090-101) following the manufacturer's instructions. Single cell suspensions were washed once with ice-cold PBS with 0.04% BSA by centrifugation at 350g and gently resuspended in fresh buffer. The cellular samples were loaded in the lane according to the 10X Genomics Chromium Next GEM Single Cell 5' v2 (Dual Index) User Guide with a single capture lane per sample. The recovery was 6,000 cells per lane. Cell partitioning completed with a uniform emulsion consistency, and the reverse transcription PCR was run. All subsequent steps of library preparation and quality control were performed as described in the 10x Genomics user guide. Then the sequencing was performed on the NovaSeq 6000 system with the NovaSeq S2 Reagent kit v1.5 (100 cycles) following the cycling parameters: 26bp (read1), 10bp (index 1), 10bp (index2), 90bp (read2).