design: Fecal samples from 48 mice (4 mice/sex/diet/genotype) in the behavioral cohort were subjected to DNA extraction and whole genome sequencing at the University of Minnesota Genomics Center. In detail, DNA was extracted from these fecal samples using DNeasy PowerSoil Pro Kits (QIAGEN, 47014) and normalized. All these DNA samples passed the quality control using the a NanoDrop spectrophotometer (Thermo Scientific, ND2000) and were subjected to library creation using NexteraXT kit (Illumina, 1/4 Rxn). All the library samples passed the quality control on MiSeq Nano (Illumina) using MiSeq Reagent Kits v2 (Illumina) and they were subjected to shotgun metagenomics on the NovSeqS1 (Illumina) at 2X150 paired end reads, yielding a sequencing depth as ~6M reads/sample.