library_construction_protocol: Cells were spun down at 300g at 4°C for 10 minutes, and digestion buffer replaced with 1X HBSS + 10% FBS. Cells were filtered into a FACS tube and incubated with anti-CD31 BV421 and anti-CD45 per-CP antibodies. CD31+ CD45- endothelial cells were sorted using a FACS Melody, collected, and resuspended in 0.1% BSA solution in 1X PBS for 10X Chromium v3 scRNAseq 10X Genomics Chromium V3 pipeline as per manufacturer instruction