library_construction_protocol: RNA was harvested using ISOGEN (Wako). 20 ng of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® (NEB, E6420) and NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2) (NEB, E6446S).