library_construction_protocol: Total RNA was purified from mid-log phase cells using Direct-zol RNA Miniprep Kit (Zymo Research) following manufacturer's instructions, including the use of ZR BashingBead Lysis Tubes to lyse open the cells in TRI Reagent. DNA was removed using DNase I (Zymo Research) and purified using RNA Clean & Concentrator (Zymo Research). Libraries were prepared using NextFlex Small RNA-seq v3 Kits (PerkinElmer).