library_construction_protocol: Total RNA was extracted using the hot-phenol method  and further cleaned up using the RNeasy mini kit (Qiagen). RNA quality was assessed by gel, spectrophotometric analysis (Spectramax Plus 384, Molecular Devices), and bioanalyzer analysis (Agilent). RNA samples sent for sequencing had a RIN > 8 and absorbance 260/280 of >2.0. RNA was quantified for sequencing by Quibit (Thermofischer Scientific). Samples were treated with DNase (RNAse free); Invitrogen. Illumina's Stranded mRNA prep with 10bp IDT for Illumina indices