design: Tomato (Solanum lycopersicum cv. M82) leaf protoplasts from 3.54 week-old seedlings, PEG-mediated CRISPRCas9 RNP transformation, amplicon library for gRNA targeting chr2;53779047;53779067;+_1st_rep (target sequence: AACCCATCATAACCATACAGAGG) prepared using high-fidelity PCR