library_construction_protocol: 8-week-old Fosl2flox/flox and Cyp19a1-Cre; Fosl2flox/flox female mice from the same litter were euthanized to collect ovaries tissues. The single-cell library was meticulously prepared using the Chromium Single Cell 3′ Library and Gel Bead Kit v3 (10×Genomics, 120237), strictly following the manufacturer's protocol. Briefly, cell viability was confirmed to be >70% in ovaries tissues from Fosl2-deficient and control mice using trypan blue exclusion. Cell suspensions were filtered using a 70 μm strainer, and dissociated cells were pelleted and re-suspended with 0.04% BSA in PBS. Single-cell suspensions were loaded on the Chromium Controller (10×Genomics, USA) to generate Gel Bead-In-Emulsions (GEMs)