library_construction_protocol: Tissues were cut while submerged in cold phosphate-buffered saline (PBS) or UW solution, washed to remove blood, transferred into 1 mL lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, 0.1% Nonidet™ P40 Substitute, and 50 U/mL RNase inhibitor in DEPC-treated water), cut into smaller pieces, and aspirated with the lysis buffer into a 50-mL tube; then, 10 mL of lysis buffer was added, the tissues were ground for 20-30 seconds, and more lysis buffer was added. The mixture was placed on ice for 10 minutes, filtered with 100-μm and 70-μm strainers, and centrifuged for 5 minutes at 700 relative centrifugal force (rcf) and 4 °C; then, the supernatant was removed, and the pellet was resuspended in 10 mL nuclei wash and resuspension buffer (1× PBS, 1.0% bovine serum albumin [BSA], and 50 U/mL RNase inhibitor). The suspension was passed through a 40-μm strainer, and the nuclei were centrifuged again for 5 minutes at 700 rcf and 4 °C; then, the supernatant was removed, the pellet was resuspended in 1 mL nuclei wash and resuspension buffer, and the nuclei were centrifuged a third time for 5 minutes at 700 rcf and 4 °C. The supernatant was removed; then, the pellet was resuspended in 5 mL sucrose cushion buffer I (2.7 mL Nuclei PURE 2 M Sucrose Cushion Solution and 300 μL Nuclei PURE Sucrose Cushion Buffer) and mixed with 10 mL sucrose buffer. The mixture was layered over 5 mL of sucrose cushion buffer in a second Eppendorf tube and then centrifuged for 60 minutes at 13000 g and 4 °C. All but 100 μL of the supernatant was removed, the nuclei were resuspended in nuclei wash and resuspension buffer, and the solution was passed through a 40-μm strainer; then, the nuclei concentration was determined with a cell counter or hemocytometer and adjusted to 1000 nuclei/μL. The nuclei were placed on ice, stained with propidium iodide for 5 minutes, and then immediately processed via the 10× Genomics® Single-Cell Protocol. The nuclei were placed on ice, stained with propidium iodide for 5 minutes, and then immediately processed via the 10× Genomics® Single-Cell Protocol.