design: rchived colonizing and invasive KPn isolates were sub-cultured and genomic DNA was extracted using standard protocols. Following extraction, NexteraXT libraries were prepared (Illumina, San Diego, CA) and sequenced on the MiSeq platform, producing 300-base paired-end reads (Illumina, San Diego, CA). After sequencing, FASTQC version v0.12.1 was used to determine the quality of the raw reads and the paired-end (PE) reads were processed with the Jekesa pipeline with -s option to identify the MLST schema kpneumoniae for KPn WGS typing. Trim Galore was used to remove adaptors, ambiguous reads, and low-quality bases (Q >30 and length >50 bp), and de novo assemblies were generated using SPAdes and Shovill packages. The assembly quality was then assessed with QUAST 5 and assembly metrics were computed.