design: Approximately 150 mg of fresh hypocotyl tissue were frozen with liquid nitrogen and powdered. RNA isolation was performed using Quick-RNA Plant Mini-Prep Kit (Zymo Research) following the protocol provided by the manufacturer with an extension to 30 min in DNAse digestion. Integrity of RNA assessment was performed through 2% agarose gel and electrophoresis run at 100V for 1 hour. Samples were sequenced in the Illumina Novaseq 6000 platform for 150 cycles in paired-end mode.