library_construction_protocol: Total RNA was extracted using the RNeasy Micro Kit (Qiagen) and the RNase-free DNase Set. RNA integrity was assessed using the Agilent Tapestation and samples with RIN >9 were used for sequencing Libraries were prepared with the Illumina TruSeq Stranded mRNA Library Prep Kit and sequenced on the Illumina NovaSeqX to ~30 million depth with 50 bp paired-end reads.