library_construction_protocol: The BD Rhapsody Single Cell platform was used for extraction and library construction, which included the Rhapsody Enhanced Cartridge Reagent Kit (BD), the BD Rhapsody Cartridge Kit (BD), the Rhapsody cDNA Kit (BD), the Rhapsody WTA Amplification Kit (BD), the Human Single-Cell Multiplexing Kit (BD), used according to manufacturer's recommendations and protocols. Cells from each donor werelabelled with sample tags and a panel of BD® AbSeq Ab-oligos (BD), washed with stain buffer, and pooled together in cold sample buffer to obtain ~60,000 cells in 620 ul for each of the pooled unstimulated and stimulated samples. Two nanowell cartridges were primed and subsequently loaded with the pooled samples. Libraries for whole transcriptome and AbSeq analysis were prepared by following the BD Rhapsody System (TCR/BCR Full Length, mRNA WTA, BD® AbSeq, and Sample Tag Library Preparation) Protocol. Quality control was performed using Agilent DNA High Sensitivity Kit (Agilent Technologies) and the Agilent 2100 Bioanalyzer (Agilent Technologies) on the intermediate and final sequencing libraries, including estimating the concentration of each sample, measuring the average fragment size of the libraries, and following sequencing recommendations. Libraries were diluted to 350-650 picomolar range per sample and sequenced using Illumina NovaSeq 150bp PE sequencing targeting 8,000M individual reads (4,000M read-pairs) with PhiX spike-in of 20%.