library_construction_protocol: For scRNA-seq, we used the 10X Genomics scRNA-seq kit v3 (PN-1000121, PN-1000128, PN-1000213) to sequence barcoded cells. We resuspended the cells (targeting ~10,000 cells for recovery per sample) in PBS and followed the protocol for the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 as per manufacturer directions (10X Genomics). For scRNA-seq, we used the 10X Genomics scRNA-seq kit v3 (PN-1000121, PN-1000128, PN-1000213) to sequence barcoded cells. We resuspended the cells (targeting ~10,000 cells for recovery per sample) in PBS and followed the protocol for the Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 as per manufacturer directions (10X Genomics). In brief, we generated gel beads-in-emulsion (GEMs) using the 10X Chromium system and subsequently extracted and amplified (11 cycles) barcoded cDNA as per post-GEM RT-cleanup instructions. We then used a fraction of this amplified cDNA (25%) and proceeded with fragmentation, end-repair, poly A-tailing, adapter ligation, and 10X sample indexing per the manufacturer's protocol. We quantified libraries using the High Sensitivity dsDNA kit (Thermo Fisher Q32854) on Qubit 2.0 Fluorometer (Thermo Fisher Q32866) and Bioanalyzer 2100 (Agilent G2939BA) analysis prior to sequencing on a NextSeq 2000 machine (Illumina) with a P3 100 kit using 28 cycles for read 1, 91 cycles for read 2, and 8 cycles for i7 index.