library_construction_protocol: 1uM dexamethasone (Sigma), and 0.5mM isobutylmethylxanthine (Sigma) in thepresence of 10% FBS until day 2. Cells were then fed with 1640 supplemented with 10% FBS and 10ug'ml insulin for 2 days, after which they were fed every other day with 1640 containing 10% FBS RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug oftotal RNA was used for the construction ofsequencing libraries