design: MOA-seq was performed on leaf samples as decribed in Savadel et al. 2023. with the following modifications: 150 mg of leaves were used to prepare 70 ul of nuclei, to which 7.8 ul of 50 U/ml MNase (Worthington) were added. During nuclei preparation, additional protease inhibitors, Pepstatin A [2 ug/mL] and Aprotinin [2 ug/mL], were added to the fixation solution. RNA was digested just after MNase treatment by adding 2.5 L of RNAse A (20mg/ml) to the stop EGTA solution used to stop the MNase reaction and incubating at room temperature for 30 min.. DNA purification was performed by extracting once with Chloroform:Isoamylalcohol (24:1) followed by purification with the NEB Monarch PCR and DNA cleanup kit following the instructions for oligonucleotide cleanup.