library_construction_protocol: ChIP was performed as described (Kim et al., 2014 Cell Reports) with slight modifications. Briefly, 40 mL of mid-log phase cells in YES were crosslinked with 1% formaldehyde for 20 minutes and quenched with 360 mM glycine and 2.4 mM Tris for 5 minutes. Whole cell extracts were then prepared with FastPrep-24 and the chromatin was sheared with BioRuptor using 15 cycles of 30s ON/30s OFF. 2 μg of antibodies were pre-conjugated to 15 μL of Pierce Protein A/G Magnetic Beads (Thermo Scientific) for ~3 hours at 4 °C before being added to the sheared chromatin and were incubated overnight at 4 °C. The beads were washed and de-crosslinked with proteinase K and overnight incubation at 65°C. ChIP DNA Clean & Concentrator (Zymo Research) was used to clean up the DNA. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB).