design: TCR beta sequencing of CD4+ Tcells infiltrating melanoma B16 control or depleted of Elp3
library_construction_protocol: Mouse CD4 T cells were sorted from melanoma samples RNA from CD4 T cells was extracted using Qiagen RNAeasy mini kit. TCR beta and beta libraries preparation was performed using Mouse TCR RNA kit (Milaboratories) according to the manufacturer protocol (first PCR amplification - 21 cycles, second PCR - 14 cycles).