title: Replicate 1 of bulk CUT&TAG of LMNB1 in H1
design: Library strategy: CUT&Tag; Library selection: CUT&Tag. First replicate of bulk CUT&Tag was performed on approximately 100,000 H1 human embryonic stem cells using an anti-Lamin B1 antibody (Abcam, ab239399). Following nuclei extraction and ConA bead binding, cells were incubated overnight with the primary antibody, followed by secondary antibody binding and indexed pA-Tn5 tagmentation. Libraries were PCR-amplified and purified before sequencing on the Illumina NovaSeq PE150 platform. Three biological replicates were processed independently.
identifiers.namespace: SUB15377797
identifiers.id: C&TLB11