title: GSM8771640: ATAC_FGO_KO_2; Mus musculus; ATAC-seq
library_construction_protocol: Each sample contains 50 oocytes collected from mated female mice at 44 hours after PMSG injection. Oocytes were washed with DNase/RNase-free 0.01% BSA and collected in DNA-lowing binding tubes. Lysis buffer (0.05% digitonin, 0.5% NP-40, and 0.5% Tween20) were added immediately after sample collection. Tn5 enzyme in Chromatin Profile Kit for Illumina (Novoprotein; N248) was used to fragment chromatin at 37℃ on Thermo Mixer (TS100) with 1000 rpm for 30 minutes and stopped by stop buffer immediately. After extraction with DNA extract beads, DNA fragments were amplified for 15 cycles using the following PCR conditions: 72℃ for 3min; 98℃ for 30 s; and thermocycling at 98℃ for 15 s, 60℃ for 15 s and 72℃ for 8 s; following by 72℃ 2 min. After the PCR reaction, libraries were purified with the 1.2x DNA clean beads following with fragment selection with 0.5x/0.7x AMPure beads (Beckman, A63882). Barcoded libraries were pooled and sequenced on the Illumina HiSeq X Ten platform using 150 bp paired-end reads
identifiers.namespace: GEO
identifiers.id: GSM8771640_r1