title: MFA of S. pneumoniae; Ptet-HU-sfGFP (+aTc, 3h)
design: Cells were pre-grown until OD600=0.3 in C+Y medium at 37C in presence of the inducer (aTc) for HU depletion strain (VL5898), and in CY only for WT D39V. Cells were then washed three times with CY and diluted 15 times in 10 ml of fresh C+Y medium supplemented with or without the inducer and harvested for genomic DNA isolation after one, two and three hours of depletion (OD600=0.1-0.2). Genomic DNA was isolated using the FastPure Bacteria, DNA isolation Mini Kit (Vazyme). Samples were sequenced with Illumina PE150 by Novogene, and, on average, 14,530,047 raw reads (maximum: 24,200,092 - minimum 12,279,628).
identifiers.namespace: SUB14752130
identifiers.id: hlpA1