title: GSM8741292: smad6ab_DKO_1, biol rep 1, 5 dpf, 5 fish pooled; Danio rerio; RNA-Seq
library_construction_protocol: For RNA extraction, the Rneasy® minikit (Qiagen; Hilden, Germany) was used following manufacturers guidelines. For each sample, a sequencing library was constructed starting from 16 ng input RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Lexogen) and the UMI Second Strand Synthesis Module for QuantSeq FWD (Illumina, Read 1). This incorporates a unique molecular identifier (UMI) in the first 6 nucleotides of each read, allowing to identify PCR duplicates and eliminate amplification bias. The libraries were sequenced as single-read 75 on an Aviti device (Element Biosciences).
identifiers.namespace: GEO
identifiers.id: GSM8741292_r1