title: GSM8839775: HeLa cells, parental, untreated, replicate 4; Homo sapiens; ssRNA-seq
library_construction_protocol: HeLa/Ferret samples: Total RNA was extracted using Trizol; for HeLa cells, genomic DNA was removed by two Dnase I treatment steps and RNA was cleaned up using phenol/chloroform/isoamyl alcohol HeLa/Ferret samples: RNA integrity numbers (RIN) were assessed by fragment analyzer and only RNAs with RIN=10 were further  processed; ribosomal RNA was removed before RNA-seq library preparation; strand-specific libraries were generated using a custom protocol at the Paul-Ehrlich-Institute (PMID 35952673) or a standard protocol by Azenta (Leipzig, Germany) HeLa, Ferret samples: Libraries were sequenced at 2x 150 bp paired-end reads
identifiers.namespace: GEO
identifiers.id: GSM8839775_r1