title: GSM9039562: lesional skin from pemphigus patient P10; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from skin biopsies using the peqGOLD Total RNA Kit (VWR International). RNA concentration and purity were assessed using NanoDrop and Qubit fluorometry. RNA integrity was determined by Agilent Bioanalyzer; samples with RIN ≥ 8.0 were processed further. Libraries were prepared using standard polyA enrichment protocols. Sequencing was performed on an Illumina NextSeq 500 platform in single-end mode (1 × 75 bp), targeting ~25 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM9039562_r1