title: GSM8877436: N2 WT, young adults, smallRNA-Seq, biol rep 1; Caenorhabditis elegans; ncRNA-Seq
library_construction_protocol: Populations of young adults were collected and washed in M9 and added to Trizol Reagent (Invitrogen). Worms in Trizol were lysed by freeze-thawing from -80 °C to 4 °C three times. Total RNA was purified from the lysate using the miRNeasy kit (Qiagen) according to manufacturer instructions, including the optional Buffer RWT wash step. Libraries with insertions between 18 - 40 bp were size selected. Illumina sequencing was performed with SE50. Library preparation and sequencing performed by Novogene Corporation.
identifiers.namespace: GEO
identifiers.id: GSM8877436_r1