title: Identification of tens of thousands of novel gene loci in the human and mouse genomes through targeted full-length long-read RNA sequencing
design: Identification of tens of thousands of novel gene loci in the human and mouse genomes through targeted full-length long-read RNA sequencing
library_construction_protocol: Mouse samples were obtained from the PRBB (Barcelona Biomedical Research Park) Animal Facility. The samples were collected from both adult and embryonic mice of the C57BL/6 strain. Adult mice were selected based on standard laboratory guidelines, and embryos were harvested at the desired developmental stages under sterile conditions. All procedures were performed in accordance with institutional animal care and ethical guidelines to ensure the welfare of the animals. Total RNA was extracted from the collected samples using protocols performed by commercial providers Ambion (Thermo Fisher Scientific) or Clontech-Takara Bio. Although the exact kit or extraction protocol used was not specified, the RNA extraction followed the manufacturers' standard procedures, ensuring high-quality and reliable RNA yield for downstream applications. CapTrap cDNA library preparation: CapTrap cDNA library preparation is a long-read sequencing protocol that selectively enriches 5'-capped transcripts by capturing the 5' caps of transcripts. This protocol uses Poly(A) tail selection to specifically target mRNA, and a 5' cap-specific oxidation reaction coupled to biotin-streptavidin to capture the 5' cap structure. The captured mRNA is then converted to cDNA and the library is prepared with 5' and 3' specific linkers to enrich for full-length libraries. CapTrap is particularly useful for studying transcription start sites, alternative splicing, and post-transcriptional modifications.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14562:CapTrap_MpreCap_TpoolA_GENCODE_ont_s