title: GSM8644328: WT, 3 dpi, biological rep2; Danio rerio; RNA-Seq
library_construction_protocol: Uninjured apex tissue or injured zone from at least five zebrafish ventricles were pooled together for each biological replicate. Total RNA was extracted from the pooled apex tissue collected in TRIzol™ Reagent as previously described [105]. At least 500 ng of extracted RNA was then reverse-transcribed with EasyScript® First-Strand cDNA Synthesis SuperMix (TransGen Biotech, AE301-02) according to manufacturer's instructions Total RNA was extracted with from uninjured ventricles and injured tissues as described above. At least five ventricles and injured tissues were pooled to together for RNA extraction and serve as one biological replicate. Two biological replicates were prepared for each time point and genotype. Extracted RNA was sent to Novogene Ltd. (Beijing, China) for library construction and bulk RNA-sequencing. Constructed libraries were sequenced on NovaSeq 6000 PE150 system and ~6 GB of raw data was collected from each sample
identifiers.namespace: GEO
identifiers.id: GSM8644328_r1