title: GSM8804392: oocyte-2; Ambystoma mexicanum; ncRNA-Seq
library_construction_protocol: For sRNA-seq, RNA was extracted from two testis and two oocyte samples of the white axolotl strain using lysis reagent (QIAzol, 79306), following the manufacturer's protocol. For EM-seq, DNA was extracted from three sperm samples of the white axolotl strain using Monarch HMW DNA Extraction Kit (NEB, T3050L) and solubilized in 10mM Tris, pH 9.0, 0.5 mM EDTA. For sRNA-seq, total RNA was size-selected for 15-40 nt fragments using 15% Novex TBE-urea gel (Invitrogen, EC68855BOX). RNA fragments were purified from the gel and resuspended in 10 μl nuclease-free water. Library preparation was performed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB, E7330L) according to the manufacturer's protocol. Sequencing was performed on an Illumina HiSeq2500 platform in 50-bp single-end read mode. For EM-seq, libraries were prepared using the NEBnext Enzymatic Methyl-seq kit (NEB, E7120L) according to the manufacturer's instructions. Sequencing was performed on an Illumina NextSeq2000 platform in 150-bp paired-end read mode.
identifiers.namespace: GEO
identifiers.id: GSM8804392_r1