title: SOX7 and SOX17 DNA binding in baseline HUVEC
design: SOX7 and SOX17 DNA binding in baseline HUVEC
library_construction_protocol: Cells were trypsinised, washed with PBS and lightly crosslinked with 0.1%  methanol-free formaldehyde for 2min and frozen down at -80C in 10% DMSO and media until library preparation. HUVEC were cultured in EBM-2 Basal Medium (CC-3156) with EGM-2 SingleQuots Supplements (CC-4176). CUT&RUN was performed with the Cell Signaling CUT&RUN assay kit according to manufactures instructions using an IgG control antibody (CST  #66362) and antibodies against SOX7 (R&D Systems, AF2766) and SOX17 (R&D Systems, AF1924). Libraries were constructed using the NEBNext Ultra II kit using the modified protocol described by Nan Liu (dx.doi.org/10.17504/protocols.io.bagaibse) and NEBNext Dual Index Multiplex Oligos for Illumina.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-13999:SOX17 repeat 1_p