title: GSM8806245: Uterus, Doxorubicin and AMH, 4 hour; Mus musculus; RNA-Seq
library_construction_protocol: One uterus per animal was pooled for each group (N = 5 per group), were cut into small pieces and placed in 2 mL of DMEM (10% FBS, 1% penicillin/streptomycin), supplemented with 21 U/mL of Papain (Worthington, LK003178) and 1 mg/mL of collagenase A (Sigma Aldrich, 10103578001), and incubated for 30 minutes at 37°C. The samples were then mechanically dissociated using the gentleMACS™ Dissociator (Miltenyi Biotec). The cell suspension was filtered, and 10,000 cells from each group were collected for next steps of scRNASeq. Samples were processed for scRNAseq according to the manufacturer's instructions using the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) (10×Genomics) and Chromium Controller (10× Genomics). Single-cell libraries were sequenced using the NovaSeq S4 (Illumina) by the Bauer Core Facility at Harvard University.
identifiers.namespace: GEO
identifiers.id: GSM8806245_r1