title: GSM8879189: day7_WT_iNs_DMOG_AraC_ENDseq; Homo sapiens; OTHER
library_construction_protocol: For S1/END-seq, to dissociate iPSC-derived iNs or mouse cortical neurons, cells were washed with PBS/5mM EDTA, treated with Trypsin/EDTA for 4 minutes, and neutralized with cold PBS/EDTA/10% FBS. After pelleting and resuspending in neutralization buffer, cells were processed for END-seq or S1-END-seq. For S1-END-seq, cells were embedded in agarose plugs, lysed with Proteinase K, treated with RNase A, and equilibrated in S1 nuclease buffer. S1 nuclease was added to the plugs, incubated on ice for 15 minutes, then at 37°C for 20 minutes before terminating the reaction with EDTA. For S1/END-seq, plugs were processed through the standard END-seq protocol.
identifiers.namespace: GEO
identifiers.id: GSM8879189_r1