title: GSM8740052: DsRed2-_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Cells were lysed in Tri Reagent for RNA extraction, and then send samples to China National Gene Bank for next steps. Library construction and sequencing were done by China National Gene Bank. The library was based on DNBSEQ Platform and used PE150 sequencing. In brief, after sample quality control, the mRNA was hybridized with oligo (dT) probe and captured by magnetic beads. The mRNA was then reverse-transcripted to first-strand DNA, which was used as a template to synthesize second-strand DNA and obtained dsDNA. The dTTP-tailed adaptor was ligated to both ends of the dsDNA fragments. The ligation product was amplified by PCR, and circularized to get single-stranded circular (ssCir) library, which was then amplified through rolling circle amplification to obtain DNA nanoball (DNB). The DNB was then loaded to flowcell, and sequenced by DNBSEQ Platform.
identifiers.namespace: GEO
identifiers.id: GSM8740052_r1