title: CD4 Donor 1 oxLDL
design: Cells were treated for 48 hours with 50 µg/ml ox-LDL or an equivalent volume of control buffer. For ChIP-sequencing the MAGnify Chromatin Immunoprecipitation System (ThermoFisher, Waltham, Massachusetts, USA) was used in accordance with the manufacturer's instructions with 2µl of antibody [total rabbit IgG or anti-H3K27ac (Abcam (Cambridge, UK) ab472)]. Sonicated but untreated lysates were used as an input control. DNA was purified with a Qiagen (Venlo, Netherlands) MinElute kit prior to TruSeq adaptor ligation.
identifiers.namespace: SUB15166938
identifiers.id: CD4_chiph3k27ac_oxldl_rep1