title: Time-course RNA-seq in developing limb bud mesenchyme cells in mice
design: Time-course RNA-seq in developing limb bud mesenchyme cells in mice
library_construction_protocol: The distal region of a limb bud was isolated from Sox9-EGFP mouse embryos. Ectoderm was removed. The isolated mesenchyme cells were further dissociated by pipetting into a single-cell suspension and sorted with FACS Aria (BD) for the Sox9-EGFP signal-positive or signal-negative populations. Total RNA was extracted from each of the sorted populations. cDNA library were prepared from 10 ng of total RNA with SMARTer Ultra Low Input RNA Kit for Sequencing v3 (Clontech) and NEBNext Ultra DNA Library Prep Kit for Illumina according to the manufacturer's protocols.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14105:Y_LHL5_GFP_neg_8643_p