title: scRNA-seq of Immunecells: CRISPRa treatment
design: ScRNA-seq was performed on FACS-sorted CD45+ cells isolated from tumors 16 days after tumor establishment. Control group were treated with AAV-DJ.GFP, experiment group were treated with AAV-DJ. miniCRISPRa-Ifn/Ifn1/Ifn4. Both the experimental group and the control group consisted of three mice. Cell numbers and viabilities were assessed by AO/EB staining and analysis in LUNA-FLTM Dual Fluorescence Cell Counter. The 20000 CD45+ cells were used for scRNA-seq library prep by following the protocol from DNBelab C4. In brief, the single-cell suspensions were converted to barcoded scRNA-seq libraries through droplet encapsulation, emulsion breakage, mRNA captured bead collection, reverse transcription, cDNA amplification and purification. Indexed sequencing libraries were constructed according to the manufacturers protocol. The resulting libraries were sequenced using a  DNBSEQT7 sequencer.
identifiers.namespace: SUB14899629
identifiers.id: scRNAseq_Immunecells_miniCRISPRa_rep1