title: Illumina NovaSeq 6000 sequencing
library_construction_protocol: bTMPseq was carried out as follows: Ten million Nucelei were isolated from third larval stage worms by grinding in liquid nitrogen and dounce homogenization. bTMP was added to the nuclei and crosslinked to DNA with UV light. DNA was purified and 1-2ug was fragmented by sonication. 20ng DNA was kept as input and the rest of the sample was mixed with Streptavidin beads and bTMP-cross linked DNA was pulled down. The DNA was purified and Illumina library construction was carried out with the NEBNext� Ultra� II DNA library Prep kit.
identifiers.namespace: Institute for Cell Biology
identifiers.id: ena-EXPERIMENT-TAB-08-11-2025-13:02:28:927-69937