title: small-RNA-seq of TraPR RISC-isolated RNA from GFP-dsRNA injected WT anemones 24-hours old gastrula replicate2
design: Nematostella zygotes were injected with dsRNA carrying the 3'-half of eGFP sequence then frozen in liquid nitrogen after 24 hours. Isolation of RISCs was performed using TraPR kit (Grentzinger et al., 2020) and RNA was extracted according to manufacturer's protocol. Libraries were prepared with NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs).
identifiers.namespace: SUB15724878
identifiers.id: TR2