title: GSM8902709: Single-cell RNA-seq of E14.5 mouse ventricular tissue, ETS1 endothelial knockout replicate 1; Mus musculus; RNA-Seq
library_construction_protocol: Ventricular tissues were enzymatically dissociated using 0.25% trypsin (Gibco) at 37 °C for 20–30 minutes with gentle pipetting to obtain a single-cell suspension. The digestion was stopped by adding DMEM supplemented with 10% fetal bovine serum (FBS). Cells were filtered through a 40-μm cell strainer, centrifuged at 400 g, and resuspended in DMEM with 2% FBS. Viability was assessed by Trypan Blue staining. Single-cell suspensions were processed using the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (10X Genomics) according to the manufacturer's instructions. Single cells were partitioned into Gel Beads-in-Emulsion (GEMs), followed by reverse transcription, cDNA amplification, fragmentation, end repair, A-tailing, adaptor ligation, and sample index PCR. Final libraries included sample indices, UMIs, barcodes, and Illumina sequencing adapters, and were sequenced on the Illumina NextSeq S4 platform.
identifiers.namespace: GEO
identifiers.id: GSM8902709_r1