title: KO control rep2 (CRX1043829; CRA016263)
library_construction_protocol: Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) protocol. After total RNA was extracted, eukaryotic mRNA was enriched, fragmented and reverse transcripted into cDNA. Then the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters.
identifiers.namespace: NGDC
identifiers.id: CRX1043829
identifiers.namespace: Nanfang Hospital, Southern Medical University
identifiers.id: DRX716437