title: RNA-Seq of mus musculus: adult male spinal cord
design: Total RNA samples were tested and then enriched for eukaryotic mRNA by Oligo(dT) magnetic beads; Add Fragmentation Buffer for mRNA fragmentation; One strand of cDNA was synthesised using a six-base random primer using mRNA as a template, followed by the synthesis of the second strand of cDNA using buffer, dNTPs, DNA polymerase I and RNase H, and cDNA purification; Purified double-stranded cDNA was subjected to end repair, base A was added, sequencing junctions were added, and fragments were screened to recover cDNA around 350 bp; PCR enrichment was performed to obtain cDNA libraries.
identifiers.namespace: SUB15185784
identifiers.id: W01161370474