title: DHA2 (CRX1265982; CRA015282)
library_construction_protocol: For lung lymphocyte isolation, fresh mouse lung tissues were cut into pieces and then digested for 45 minutes at 37\032 in Hank's solution containing 10% FBS, 1 mg/ml collagenase I (Sigma-Aldrich, St. Louis, MO, United States), 1 mg/ml collagenase II (Gibco, Waltham, MA, United States) and 25 \032g/ml DNase I (Sigma-Aldrich, St. Louis, MO, United States). After digestion, the cells were resuspended in 20% Percoll in PBS and the pellets were collected after centrifugation (450g, room temperature, 10 minutes). Finally, the cells were suspended at a density of 1000 cells/\032L in PBS and about 10,000 cells were loaded onto a ChromiumTM Controller instrument after completion of a cell suspension preparation procedure using GemCode Gel Bead and Chip (10X Genomics, Pleasanton, CA) following the manufacturer's recommendations. Libraries were prepared using 10X Genomics Library Kits and sequenced on an Illumina NovaSeq 6000 to generate 150-bp paired-end reads according to the manufacturer instructions (Berry Genomics, Beijing, China).
identifiers.namespace: Army Medical University (Third Military Medical University)
identifiers.id: DRX715893
identifiers.namespace: NGDC
identifiers.id: CRX1265982