title: GSM8664471: Directed harvested X.kitaake roots protoplasting Rep #1; Oryza sativa; RNA-Seq
library_construction_protocol: Intacted roots (directly harvested from gel grown conditions) and digested roots (protoplasts + undigested chunks) were havested. Samples were frozen in liquid nitrogen and RNA was isolated using the Zymo MagBead RNA Isolation kit. RNA was used as input into the Lexogen QuantSeq 3′ FWD RNA-Seq library preparation procedure. Libraries were indexed and pooled on an Illumina NextSeq. Reads were aligned to Michigan State University Rice genome v7 counted with HTSeq-Count. RNA was used as input into the Lexogen QuantSeq 3′ FWD RNA-Seq library preparation procedure according to the manufacturer's protocol, using the Unique Molecular Identifier (UMI) PCR add-on kit. Libraries were indexed and pooled on an Illumina NextSeq. Lexogen QuantSeq 3′ FWD RNA-Seq
identifiers.namespace: GEO
identifiers.id: GSM8664471_r1