title: GSM8976593: Substantia nigra, PFF, hypoxia, rep10; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from dissected fresh frozen SN samples using Qiagen RNeasy Plus Universal mini kit following the manufacturer's instructions (Qiagen, Hilden, Germany). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were initially enriched with Oligod(T) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First-strand and second-strand cDNA were subsequently synthesized. cDNA fragments were end-repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by PCR with 13 cycles.
identifiers.namespace: GEO
identifiers.id: GSM8976593_r1