title: WGBS of rattus norvegicus: adult caudal sperms
design: Genomic DNA was extracted from 10 million caudal sperms using the HiPurA Sperm Genomic DNA Purification Kit (HiMedia, India) as per manufacturers instructions. DNA samples (with 260/280 ratios of 1.71.8) of 3 animals per group were pooled and sonicated to 200-400 bp using Covaris S220 sonicator. The fragment size distribution was checked on Agilent Bioanalyzer 2100 (Agilent Technologies, USA). This was followed by end repair, dA tailing, sequencing adaptor ligation and bisulphite conversion by EZ DNA Methylation-Gold Kit (Zymo Research). Adapter ligated-DNA was purified by size selection using AMPure bead-based method and used for library preparation using Accel-NGS Methyl-Seq DNA Library Kit from Swift BioSciences. The library prepared were quantitated using Qubit 2.0 Fluorometer (Life Technologies) and was further quantified by qPCR. All the samples were found to have library concentration more than 2 nM. The quantified libraries were pooled in equimolar amounts for multiplexed 150 bp paired end sequencing on Illumina HiSeq 2500 platform.
identifiers.namespace: SUB14769086
identifiers.id: DPN