title: small-RNA-seq of TraPR RISC-isolated RNA from uninjected WT anemones 24-hours old gastrula replicate1
design: Nematostella embryos were frozen in liquid nitrogen at 24 hours post fertilization. Isolation of RISCs was performed using TraPR kit (Grentzinger et al., 2020) and RNA was extracted according to manufacturer's protocol. Libraries were prepared with NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs).
identifiers.namespace: SUB15724878
identifiers.id: TRU1