title: Methanogenic biofilms attached to nylon pad at the end of stage III (biodegradability tests).
design: Amplification of V4V5 of the prokaryotic 16S rRNA genes was realized using 515532F (5-GTGYCAGCMGCCGCGGTA-3) and 909928R (5-ACTYAAAKGAATTGRCGGGG-3) primers. The resulting products were purified for sequencing by using an Illumina MiSeq platform. Thereafter, the sequences were processed with Mothur (version 1.44) developed by INRAE. During this procedure, barcodes, primers, chimeras, and sequences with homopolymers greater than 8 bp were removed from the sequence files throughout filtering steps. Sequences with 100% homology between them were grouped into unique sequences to obtain OTUs.
identifiers.namespace: SUB14770991
identifiers.id: C25