title: 2nd gen seq of viral fraction, sample V8B
design: FeCl3 flocculation was used to concentrate virus particles from the 0.22-m filtrate, which were collected on a 0.8-m filter. Virion particles were resuspended from the 0.8-m filters using ascorbate-EDTA buffer and concentrated using 100KDa Amicon filters. DNA was then extracted using the phenol-chloroform/isoamyl method. Viral DNA was amplified using the Illustra Ready-To-Go GenomiPhi V3 DNA Amplification Kit, then sequenced using the Illumina NovaSeq 6000 platform.
identifiers.namespace: SUB15203066
identifiers.id: V8B-illumina