title: Genome sequencing of laboratory strains
design: Parasites were liberated from host cells by saponin lysis. Genomic DNA was extracted by cell lysis with SDS/proteinase K, followed by phenol:chloroform extraction and ethanol precipitation. Genomic DNA pellets were resuspended in 10 mM Tris, 1 mM EDTA and assessed by agarose gel electrophoresis and Nanodrop spectrometry. The purified genomic DNA samples were shipped on dry ice to NovogeneAIT (Singapore) for whole-genome sequencing service.
identifiers.namespace: SUB14805570
identifiers.id: TM4