title: GSM8806810: Skin, WT, Non-lesional, NoTx, Sample 10; Mus musculus; RNA-Seq
library_construction_protocol: Skin samples were isolated from lesional skin in 12-month-old Ifnk TG mice and from non-lesional skin in both Ifnk TG mice and WT mice. For isolation of RNA from skin tissue, frozen samples were crushed with mortar and pestle prior to homogenization using a handhold tissue homogenizer (Grainger). RNA isolation of skin was done using  For lymph node RNA isolation, inguinal skin-draining lymph nodes were put through a 70µm cell strainer. Homogenates were then lysed in RLT lysis buffer and RNA was purified using RNeasy Plus Mini Kit (Qiagen). Total RNA quantity and purity were determined based on A260nm/A280 nm using a BIOTEK EPOCH plate reader. This pool was subjected to 151bp paired-end sequencing according to the manufacturer's protocol (Illumina NovaSeqXPlus). BCL Convert Conversion Software v4.0 (Illumina) was used to generate de-multiplexed Fastq files.
identifiers.namespace: GEO
identifiers.id: GSM8806810_r1