title: GSM8840112: isoG256W-3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the Rneasy Mini kit. RNA samples were checked on the Agilent Bioanalyzer 2100 RNA Nano chip for RNA integrity (RIN > 7) and quantified using the Qubit RNA HS Assay. RNA library preparation was performed following the NEBNext Ultra II Directional RNA Library Preparation protocol to generate stranded data. In brief, 200 ng of total RNA was used as the input material and enriched for poly-A mRNA using magnetic oligo d(T) beads, fragmented into the 200-300-bases range for 10 minutes at 94°C and converted to double stranded cDNA. cDNA proceeded to library preparation and dual-index Illumina adapters were added through PCR. Final libraries were validated on the Fragment Analyzer using the High Sensitivity NGS Kit or on the Bioanalyzer 2100 DNA High Sensitivity chip to check for size and absence of primer dimers and quantified by qPCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol.
identifiers.namespace: GEO
identifiers.id: GSM8840112_r1