title: metagenome of environmental virus: mangrove sediment
design: After relatively pure virus-like particle (VLP) samples were obtained from the sediment, viral DNA (dsDNA, ssDNA) was extracted. Then, different library construction methods were selected according to different viral genome types: dsDNA genome can directly construct DNA library, while ssDNA needs to synthesize double-stranded DNA before DNA library construction. Qualified DNA samples or amplified DNA samples were randomly interrupted by ultrasonic crusher, and the short DNA fragments obtained after interruption were used for sequencing library construction. Qualified DNA libraries were sequenced by Illumina platform with a sequencing read length of PE150.
identifiers.namespace: SUB14834622
identifiers.id: LD240816O-1902A