title: GSM8771449: Microglia GEX; Mus musculus; RNA-Seq
library_construction_protocol: Dissociated brains were subjected to Percoll purification and subsequently FACS sorted on CD45+ cells For single cell library preparation, cells were loaded onto a Chromium Single Cell 3′ G Chip (10X Genomics) according to the manufacturer's instructions without modifications using Chromium Next GEM Single Cell 3' Reagents Kit v3.1 (10X Genomics). The 6 samples were multiplexed using Cell Multiplex Oligos (CMOs) for CellPlex reagents. Dual Index Plate NN Set A (10x Genomics) was used for Cell Multiplexing library construction, Dual Index Plate TT Set A was used for Gene Expression library construction according to the manufacturer's instructions. The cDNA content and size of post-sample index PCR samples was analyzed using a 2100 BioAnalyzer (Agilent). Library quantification was done using NEB Next® Library Quant Kit for Illumina® (New England Biolabs) following manufacturer's instructions. Sequencing libraries were loaded on an Illumina NextSeq 1000 P2 flow cell, with sequencing settings according to the recommendations of 10× Genomics (read 1: 28 cycles, read 2: 90 cycles, i7 index: 10 cycles, i5 index: 10 cycles).
identifiers.namespace: GEO
identifiers.id: GSM8771449_r1