title: RNA-Seq of birds
design: RNA Extraction with MagMax Viral Pathogen Nucleic Acid Isolation Kit (Applied Biosystems), reverse transcribed using the SuperScript VILO MasterMix Kit (Thermo Scientific); conversion to ds-cDNA with the Neb-Next Second Strand Synthesis Module (NEB Biologicals); purification with the PureLink PCR Purification Kit (Thermo Scientific) and quantified with the  Qubit Assay DNA HS kit on the Qubit 4.0  instrument (Thermo Scientific). After genomic library synthesis with the Nextera XT DNA kit, samples were sequenced  on a NextSeq 500 platform (Illumina Inc.) using the NextSeq 500/550 High Output kit v2.5 (300 cycles).
identifiers.namespace: SUB15199388
identifiers.id: JARP8