title: Metataxonomic and immunological analysis of feces from chil-dren with or without Phelan-McDermid syndrome
design: The hypervariable V3-V4 region of the bacterial 16S rRNA gene was amplified using PCR. Equimolar concentrations of the universal primers S-D-Bact-0341-b-S-17 and S-D-Bact-0785-a-A-21 were used. PCR products were pooled in equimolar DNA concentrations and run on an agarose gel. Bands were extracted and purified using the QIAEX II Gel Extraction Kit (QIAgen) and quantified with PicoGreen (BMG Labtech, Jena, Germany). Aliquots of the amplicons were sequenced using the paired-end sequencing protocol on the Illumina MiSeq sequencer (Illumina Inc., San Diego, CA) at the Scientific Park of Madrid, Spain. Sequences were demultiplexed using Illumina software (version 2.6.2.3) following the manufacturer's guidelines.
identifiers.namespace: SUB14701764
identifiers.id: Phe8