title: RNA-seq of mus musculus: P11 Ki-67 ctrl cerebellum rep 3
design: Isolated mouse cerebellar nuclei were homogenized in Trizol. Total nuclear RNA was isolated using the column-based Qiagen RNeasy kit. Library prep was performed using 1 ug of input RNA with the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit. After depletion of rRNA and cDNA synthesis, the DNA was quantitated using Qubit and equalized acrosssamples. Illumina-compatible dual index primers were used during 15 PCR cycles. The prepared libraries were subsequently sequencing using the Illumina NovaSeq X Plus platform to generate 100 bp paired-end reads.
identifiers.namespace: SUB15172412
identifiers.id: CJF26_UDP0053V3