title: GSM8701565: SH_WT_3; Danio rerio; RNA-Seq
library_construction_protocol: Heart ventricles dissected out from cryoinjured and sham-operated wt and ankrd1a mutant fish were homogenized in TRIzol Reagent (Invitorgen, Thermo Fisher Scientific, USA). Three ventricles were pooled as one sample. Total RNA was extracted using RNA Clean and concentrator kit (ZYMO Research, USA), according to the manufacturer instructions and eluted in 23 µl of RNase-free water and its quantity and purity were measured promptly after isolation, using BioSpec-nano (Shimadzu, Japan) and Qubit 4TM Fluorometer with ssRNA BR Assay KitTM (Thermo Scientific Fisher, USA). cDNA libraries were constructed via poly A enrichment using the Illumina TruSeq Stranded mRNA Library Preparation kit (Illumina, California, USA)
identifiers.namespace: GEO
identifiers.id: GSM8701565_r1