title: GSM8583526: cFb_Old_MI_rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from PBS-washed cell pellets using miRNeasy Mini Kit (Qiagen). The samples were homogenized in 1 mL Qiazol (Qiagen) using a vortex, followed by incubation at room temperature for 5 minutes. Chloroform was added to the lysates followed by cooled centrifugation at 12,000 g for 15 minutes at 4°C. Exactly 650 µL of the upper aqueous phase was mixed with 750 µL ethanol and RNA was precipitated on a miRNeasy mini column followed by automated washing with RPE and RWT buffer in a Qiacube liquid handling robot. Finally, total RNA was eluted in 30 µL nuclease free water. Total RNA integrity was checked using the RNA 6000 Nano Assay (Agilent, CA), as well as spectrophotometric RNA quantification (Nanodrop). All samples showed RIN values between 9.4 to 10, except for one sample with a RIN of 6.6. library construction and sequencing was done by Novogene
identifiers.namespace: GEO
identifiers.id: GSM8583526_r1