title: RNA-seq of large yellow croakeroverexpression of Lclectin in macrophages
design: Total RNA was extracted from cells transfected with pcDNA3.1 and pcDNA3.1- LcLectin recombinant plasmid, following the protocol outlined in the TransZol Up Plus RNA Kit (TransGen, China). The qualified RNA samples were subsequently utilized for library construction. Subsequently, target fragments were enriched through PCR amplification, and libraries approximately 450 bp in size were selected for quality assessment and concentration measurement. Libraries with different indices were then pooled based on effective concentration and data requirements, diluted to 2 nM, and denatured to generate single-stranded libraries. Finally, paired-end (PE) sequencing was carried out on the Illumina NovaSeq 6000 platform
identifiers.namespace: SUB14871333
identifiers.id: LRA223550