title: 16S rRNA sequencing of Abies pindrow: Seed microbiome
design: DNA was extracted using the Qiagen DNeasy Plant Mini Kit. The V3-V4 region of the 16S rRNA gene was amplified with primers 341F and 785R, then barcoded, purified, and quantified. Paired-end sequencing (2300 bp) was performed on an Illumina MiSeq platform. Raw reads were quality-checked, with only high-quality data used.
identifiers.namespace: SUB15753691
identifiers.id: LIB_SB2_16S