title: GSM8701350: In vitro differentiated murine pathogenic TH17 cells treated with NorUDCA for 4 hours replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: RNA was harvested using RNEASY mini kit (Qiagen). Around 250 nanogram total RNA were used for the construction of sequencing libraries. The amount of total RNA was quantified using the Qubit 2.0 Fluorometric Quantitation assay (Thermo Fisher Scientific, Waltham, MA, USA) and the RNA integrity number (RIN) was determined using the 2100 Bioanalyzer High-Resolution Automated Electrophoresis instrument (Agilent, Santa Clara, CA, USA). RNA-seq libraries were prepared with the NEBNext UltraExpress® RNA Library Prep Kit (New England Biolabs, Inc., Ipswich, MA, USA). NGS library concentrations were quantified with the Qubit 2.0 assay and the size distribution was assessed using the 2100 Bioanalyzer instrument. Before sequencing, sample-specific NGS libraries were diluted and pooled in equimolar amounts.
identifiers.namespace: GEO
identifiers.id: GSM8701350_r1