title: ITS illumina seq of root
design: Libraries were prepared at the NERC Environmental Omics Facility in Sheffield, UK. After DNA extraction, primary PCRs were carried out using 16S primers 515F and 806R. Then, PCR products were cleaned with Promega Pronex Beads and PCR2 was carried out to add unique identifier sequences. Then, DNA concentrations were quantified and samples were pooled, followed by another bead cleaning. Next, qPCR was performed and samples were pooled into the final library.
identifiers.namespace: SUB15768907
identifiers.id: Sample 69_NO SMX_root