title: GSM8740104: PC9_M7A_OE_input_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq was performed using the Active Motif ChIP-IT Express Kit (Active Motif 53008) according to the manufacturer's instructions. Approximately 2 x 107 cells were used per biological replicate. Chromatin was sheared using a Covaris S220 using a peak power of 175W, duty factor of 10%, 200 cycles/burst, and 160 seconds per run. One uL of decrosslinked chromatin was analyzed via Agilent TapeStation to determine fragment size, and DNA concentration was measured using Qubit (Invitrogen Q32854). c-MYC-tag (Cell Signaling Technologies 2276S) antibody was used. 20 ug of chromatin was pre-incubated with antibody overnight at 4ºC. Protein G beads were subsequently added to antibody-bound chromatin and incubated for 4 hours at 4ºC. 2.5 ng of immunoprecipitated chromatin was subject to library preparation using EpiCypher CUT&RUN Library Kit (EpiCypher 141001) according to the manufacturer's instructions. Pooled libraries were sequenced by Novogene with NovaSeq 6000 with 20 million paired-end reads sequenced per library.
identifiers.namespace: GEO
identifiers.id: GSM8740104_r1