title: GSM8877053: YM2_ATAC; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq wash buffer was added to thaw cryopreserved FISCs (~60,000 cells each sample) and pelleted by centrifugation at 500g for 5 min at 4 °C. The pellet was resuspended in 50 μl of ice-cold ATAC-seq lysis buffer and incubated on ice for 3 min. The cell lysate was added with 1 ml of ATAC-seq wash buffer followed by centrifugation at 500g for 10 min at 4°C. The cell pellet was resuspended in transposition mix including 2.5 μl of Tagment DNA enzyme 1 (Illumina), 0.1% Tween 20, and 0.01% digitonin and incubated at 37°C for 30 min on thermomixer at 1000 rpm. The transposed DNA fragments were PCR-amplified using NEBNext Ultra II Q5 2× Master Mix (NEB). The sample was purified using the DNA Clean and Concentrator-5 Kit (Zymo). The library quality and quantity were verified using the high sensitivity DNA kit on a 2100 Bioanalyzer (Agilent) and qPCR with NEBNext Library Quant kit (NEB) respectively. The ATAC libraries were subjected to 50 bp paired end sequencing on a NextSeq 2000 platform using a P2 100-cycle kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM8877053_r1