title: SUC_5 (CRX700214; CRA010900)
library_construction_protocol: Metagenomic libraries were prepared following TruSeqTM Nano DNA sample preparation Kit from Illumina (San Diego, CA), using 1ug of total DNA. DNA end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illumina's protocol. Libraries were then size selected for DNA target fragments of ~400bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles.
identifiers.namespace: Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences
identifiers.id: DRX710599
identifiers.namespace: NGDC
identifiers.id: CRX700214