title: C_fragile_RNA-Seq
design: Codium fragile were collected from Masonboro Sound at the University of North Carolina Wilmingtons Center for Marine Science, June 2021.  Thallus tissue was frozen in liquid nitrogen and ground into powders with mortars and pestles.  RLT extraction buffer from Qiagens RNeasy Plant Mini kit (Germantown, MD, USA) was pipetted into each mortar and mixed into the frozen powder with a pestle as it thawed. Each slurry was transferred to a microfuge tube and RNA extracted according to the manufacturers protocol. Total RNA sequencing (RNA-Seq) was completed by Azenta using Illumina 2x150bp technology with DNase treatment and ribosome depletion. . They were transported to UVA Wise in seawater, cultured approximately 16 hours under artificial light, and RNA was extracted.
identifiers.namespace: SUB15210023
identifiers.id: C_fragile_RNA-Seq