title: RNA-seq of Periplaneta americana digestive tissues
design: Tissues were stored in RNAlater at -80C upon dissection. For RNA extraction, tissues were homogenized via rotor stator homogenizer in Omega Biotek's RNA-solv reagent, after which chloroform phase separation was performed and the RNA-containing aqueous phase was added to Omega Biotek's RNA kit II column-based clean-up reagents. RNA was further cleaned with Turbo DNase treatment and assessed for quality on an Agilent Bioanalyzer using the Agilent RNA Pico chip kit. The stranded NEB RNA library prep kit was used with unique barcode combinations and polyA selection to generate transcriptome libraries.
identifiers.namespace: SUB15050465
identifiers.id: XT5_MgT6