title: GSM8976635: RiboTag, spast, postnatal day 9, replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Spinal cords were homogenized (buffer in RNase-free water in mM: 50 TrisCl, pH 7.5, 100 KCl, 12 MgCl2, 1 % NP-40, 1 X dithiothreitol (DTT), 1 X protease inhibitors, 1 mg/ml heparin, 100 µg/ml cycloheximide, RNAseOUT. The mRNA-ribosome complex was precipitated using a polyclonal HA-antibody (Sigma #H6908) and Dynabeads Protein G. Ribosome-bound mRNA was isolated with RNeasy Plus Micro Kit (Qiagen #74034) per manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8976635_r1