title: RNA-Seq of mus musculus: brain
design: One microgram of total RNA was used for the library preparation using oligo(dT) capture beads (Illumina) according to the manufacturer's instructions. The double-stranded cDNA was treated for ends repair, dA-tailing, and adaptor ligation. Size selection of adaptor-ligated DNA was performed using DNA clean beads. Each sample was amplified by PCR using primers P5: 5' AAT GAT ACG GCG ACC ACC GA 3' and P7: 5' CAA GCA GAA GAC GGC ATA CGA GAT 3'. Libraries with different indexes were multiplexed and sequenced using a 2150 paired-end sequencing configuration (Illumina).
identifiers.namespace: SUB14773261
identifiers.id: ACLJ3