title: 16S rRNA sequencing of Withania somnifera root sample
design: Extracted DNA from  sample was subjected to PCR amplification usingbarcoded 16S V1-V9 region specific primers and LongAmp (NEB) Taq 2X master mix,following the protocol outlined in the 16S Barcoding Kit 24 V14 (SQK-16S114.24; OxfordNanopore). The amplicons contain 5 tags which facilitate the ligase-free attachment ofRapid Sequencing Adapters. The amplicons generated from the samples were confirmedusing 1% agarose gel electrophoresis stained with EtBr. The PCR products were purified byusing 1.6X Ampure XP beads (Beckmann Coulter, USA). The amplicons from each samplewere quantified using Qubit 4 Fluorometer (Thermo Fisher Scientific, USA) and ~50 ng fromeach sample were pooled. The pooled amplicons were subjected to library clean-up usingthe 1X AmPure XP beads (Beckmann Coulter, USA) supplied in the 16S Barcoding Kit 24V14. Sequencing was performed on PromethION 2 Solo platform (Oxford NanoporeTechnologies, Oxford, UK) using R10.4.1 flow cells (FLO-PRO114) on MinKNOW (version24.06.14, ONT).
identifiers.namespace: SUB15653524
identifiers.id: Root