title: CKA15 ont-cappable-seq control reads
design: ONT-cappable-seq library preparation involves purification and DNase treatment of total RNA, followed by poly A-tailing and desthiobiotin(DTB)-GTP capping of the RNA. After capping and polyA tailing, the resulting RNA is split into two workflows, one corresponding to the enriched fraction and another to the control. In the enriched workflow, primary transcript enrichment is performed using streptavidin magnetic beads, followed by first-stand cDNA synthesis, selection of full-length cDNAs, second-strand synthesis, PCR barcoding and amplification, and adaptor ligation. The primary transcript enrichment and selection of full-length cDNA by streptavidin enrichment steps are omitted in the control RNA.
identifiers.namespace: SUB14769487
identifiers.id: barcode09