title: GSM8736869: Replicate 2 of scRNAseq of bone marrow immune cells, HTO; Mus musculus; OTHER
library_construction_protocol: Bone marrow cells were then sorted into PBS with 0.02% BSA to exclude erythrocytes and dead cells as well as limit the numbers of abundant immune cell types such as neutrophils, monocytes and T and B cells. In brief, 30,000 target cells from each panel were sort-purified independently from  PBS and SA treated mice using a FACSAria Fusion flow cytometer (BD). Subsequently, 3 and 4 samples were pooled per replicate experiment respectively (Replicate experiment 1 : MR_30_10X_SA_PBS_BM_22112021_Transcriptome, replicate experiment 2: MR_33_10X_SA_PBS_BM_Transcriptome). Those  cell fractions were pooled . for processing as a single sample according to the manufacturer's protocol, while the antibody-linked barcodes enabled sample-specific demultiplexing of the sequencing data. Both scRNA-seq libraries were generated using the Chromium Controller and the Next GEM Single Cell 3' Reagent Kit (v3, 10x Genomics) according to the manufacturer's instructions
identifiers.namespace: GEO
identifiers.id: GSM8736869_r1