title: Triticum aestivum /'Agropyron glael  subsitution line 3St(3D)
design: Genomic DNA was extracted from fresh young leaves of wheat donor genotypes MV9 and KAR, the parental Thinopyrum hybrid GLAEL, and the GLA7 line in 96 well plates using BioSprint DNA kit (Qiagen Inc.) following the manufacturers protocol. As the basis for double-digest restriction site-associated DNA (ddRAD) library protocol development, the protocol of Yang et al. (2016) with minor modification was followed: (1) restriction enzymes MspI and SphI were used; (2) the sequences of P1 and P2 adapters with barcodes and primers with indexes were designed according to Poland et al. (2012); (3) pooled samples were size-selected for fragment sizes of 350 to 390 bp using a dye-free Blue Pippin 1.5% gel cassette (BDF1510) (Sage Science, Beverly, MA, USA). All sublibraries were pooled equimolarly and sequenced on the NovaSeq 6000 platform (Illumina) using the SP reagent kit v.1.5 in 2  150 bp configuration.
identifiers.namespace: SUB15237464
identifiers.id: 1B4