title: GSM8806444: Caki-MX_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted by combining the TRIzol protocol (Invitrogen; Carlsbad, CA) with the QIAGEN RNeasy mini kit (QIAGEN, Carlsbad, CA). Briefly, 1x107 cells were lysed with 0.65ml TRIzol reagent for 10 minutes at room temperature before adding 300ul chloroform. The mixture was vortexed for 15 seconds, kept static for 3 minutes, and then centrifuged samples at 12,000 rpm for 3 min at room temperature. The supernatant was carefully transferred into another DNase/RNase-free centrifuge tube, mixed well with an equal volume of 70% ethanol by pipetting, and loaded to the RNeasy Mini spin column provided in the QIAGEN RNeasy mini kit. The column with samples was kept static for 2 minutes before centrifuging for 15 s at 12,000rpm. Discard the flow-through. After washing, the spin column was placed in a new 2 ml collection tube and dried by spinning at 15,000 rpm for 5 min. RNA on the membrane will be eluted with 40μl RNase-free water (supplied in kit). High-quality libraries were prepared following Illumina Stranded mRNA Prep Manual
identifiers.namespace: GEO
identifiers.id: GSM8806444_r1