title: GSM9038695: Dura_Young_Naive; Mus musculus; RNA-Seq
library_construction_protocol: The skin overlying the skull was removed and mice were decapitated to separate the skull from the spinal column. The skull cap was then carefully separated from the brain by making incisions from the occipital to the frontal bone to the using fine surgical scissors. The skull cap was placed in a petri dish with 1 mL of PBS. Briefly, the dura mater was scored off the skull cap using forceps to release attachment points between the dura and the skull cap. The dura 1mL of digestion buffer [RPMI 1640 (Sigma-Aldrich) supplemented with 2% fetal bovine serum (Gibco), 1 mM Hepes (Corning), 1× penicillin/streptomycin (Gibco), and 1× GlutaMAX (Gibco)].  Digestion enzymes DNase I (dura: 0.5 mg/mL, LM: 60 μg/mL, Sigma-Aldrich) and collagenase P (1 mg/ml, Roche) were added. Tubes were mixed by gentle vortex and digestion allowed to proceed for 15 min at 37°C with gentle agitation. The digestion was stopped with 1 mL of PBS and samples were filtered through 35-μm mesh strainer caps into 5-ml FACS tubes prior to downstream applications. Library prep was performed according to manufacturer's instructions (10X Genomics 3' Next-GEM chemistry)
identifiers.namespace: GEO
identifiers.id: GSM9038695_r1