title: CITE-seq of 52 matched primary AML samples from diagnosis and relapse
design: CITE-seq of 52 matched primary AML samples from diagnosis and relapse
library_construction_protocol: Bone marrow mononuclear cells (BMMCs) were isolated by standard Ficoll purification methods and viably frozen from bone marrow biopsies obtained from patients with AML. Cells were loaded at a concentration of 18,000 for a targeted cell capture of 10,000 cells using the Chromium Next GEM Automated Single Cell 5' Kit v2 and Chromium Next GEM Chip K Automated Single Cell Kit (10X Genomics). Cells were loaded at a concentration of 18,000 for a targeted cell capture of 10,000 cells using the Chromium Next GEM Automated Single Cell 5' Kit v2 and Chromium Next GEM Chip K Automated Single Cell Kit (10X Genomics). Single-cell emulsions were then prepared for sequencing according to the manufacturer's protocol (10X Genomics)
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16321:P03D_5GEX_p