title: Fix, Hex Rep1 (CRX716402; CRA011370)
library_construction_protocol: PCR was performed to amplify the library for 5 cycles using KAPA HiFi PCR Kit (Kapa Biosystems, kk2102)  with the following PCR conditions: 72degreeC for 5min; 98degreeC for 3mins; and thermocycling at 98degreeC for 20s, 63degreeC for 30s. The determine the optimal amplification for each sample, 5ul PCR mix was diluted with 5ul H2O (Thermo Scientific, 10977015) and combined with 2x SYBR qPCR supermix (Novoprotein, E096) to perform qPCR with following conditions: 98degreeC for 3mins; and thermocycling for 30 cycles at 98degreeC for 20s, 63degreeC for 30s and 72degreeC for 1mins. The additional number of PCR rounds (N) was determined as the qPCR cycle that reached the 1/3 maximum abundance on qPCR amplification curve. All samples were amplified for another N+3 cycles with the following PCR conditions: 98degreeC for 3mins; thermocycling at 98degreeC for 20s, 63degreeC for 30s and 72degreeC for 3min; and 72degreeC for 5mins for final extension. The library was sequenced on Illumina HiSeq X Ten with an average depth of 17.5 million reads per library. submitted_instrument_model: Illumina HiSeq X Ten
identifiers.namespace: NGDC
identifiers.id: CRX716402
identifiers.namespace: Tsinghua University
identifiers.id: DRX712482