title: HEK293FT_TargetSeq_Sample_559: HEK293FT-REPAIRX_PPIB-2;HEK293FT-REPAIRX_KRAS-1;HEK293FT-REPAIRX_RAB7A-1
design: Target site sequences were PCR-amplified using high-fidelity DNA polymerase PrimeSTAR HS (Clonetech) with primer sets flanking the examined target sites. Indexed DNA libraries were prepared utilizing the NEBNext Ultra II FS DNA Library Prep (NEB). Following quantification using the Qubit dsDNA HS Assay Kit (ABP Biosciences), PCR products with different tags were pooled together for high-throughput sequencing using the Illumina NovaSeq 6000 system (2150) at Sequanta Technologies, Shanghai, China.
identifiers.namespace: SUB14955279
identifiers.id: HEK293FT_TargetSeq_Sample_559