title: GSM8839581: 24JK Tumors (Day16) rep 5; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were finely sliced and enzymatically digested in DMEM containing 0.02 mg ml−1 DNase (Sigma Aldrich) and 1 mg ml−1 type IV collagenase (Sigma Aldrich). Digestion was performed in a rocking incubator at 120 r.p.m. for 30 min at 37 °C. Following digestion, cells were filtered through a 70μm filter twice and resuspended in Fc receptor block before being stained with fluorochrome-conjugated antibodies. Single cell suspensions from digested tumors were processed with V3 chemistry using the Chromium Next GEM Single Cell 3' Gel Bead Kit v3.1 (10X Genomics) and Chromium Next GEM Single Cell Library Prep Kit (10X Genomics), according to manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8839581_r1