title: RNA From seeds of cultivar F15 at thick developmental stage grown under well-watered conditions
design: When quinoa plants reached the thick stage (85 at BBCH scale), panicles  were collected and frozen in liquid nitrogen. Seed RNA extraction was carried out using 100 mg of macerated seeds following a modified version of the procedure described by Oate-Snchez & Vicente-Carbajosa 2008) with some modifications. Briefly, ground seed tissue was homogenised in an extraction buffer containing LiCl 0.4M, Tris-HCl 0.2M pH:8, EDTA 25 mM and SDS 1%, followed by sequential extractions with chloroform and phenol-chloroform to remove proteins and other contaminants. RNA was precipitated using LiCl 8M at -20 overnight, resuspended in DEPC-treated water, and further purified by removing polysaccharides with sodium acetate and ethanol washes. Finally, DNase treatment was performed using the TURBO DNA-free Kit (Invitrogen, ThermoFisher) according to the manufacturers instructions.
identifiers.namespace: SUB14584803
identifiers.id: F15_WW_Thick_Seeds_1