title: GSM8902922: HuH-7, anti-H3K4me3, DOT1L_KO, rep2, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: For ChIP-seq experiments, cells were cross-linked with 1% formaldehyde at room temperature, followed by quenching with glycine. After cell lysis, the chromatin was sonicated for 100-500 bp at 4°C. For fanChIP assays, cells were lysed with CSK buffer. After that, the cell lysate was resuspended with MNase buffer and incubated with 0.5 unit of MNase at 37°C for 5 mins. ChIP-seq or fanChIP libraries were prepared using the VAHTS Universal DNA Library Prep Kit for Illumina from Vazyme. The purified DNA was end-repaired and then ligated with adapters. After purified by AMPure XP beads from Beckman, the DNA was amplified using 13 cycles with the PCR program.
identifiers.namespace: GEO
identifiers.id: GSM8902922_r1