title: GSM8877691: Whole placenta, Bulk RNA-seq, 24; Mus musculus; RNA-Seq
library_construction_protocol: 35-45mg of placental tissue was isolated from 12 control and 12 metformin treated placentas. RNA was extracted using the miRNeasy micro kit (QIAGEN), with DNA contamination removed using the DNAse kit (QIAGEN). Both steps were performed according to the manufacturer's instructions. RNA quality and concentration was assessed by nanodrop analysis. RNA integrity (RIN) of > 8 for all samples was confirmed by analysis with the Agilent Bioanalyser 2100 system (Agilent RNA 6000 Nano Kit). 400ng of total RNA was used for library construction (TruSeq Stranded mRNA Library Pre Kit; Illumina). Indexed libraries were normalized, pooled and sequenced on a NovaSeq 6000 (50bp paired-end).
identifiers.namespace: GEO
identifiers.id: GSM8877691_r1