title: Metagenomic DNA form ruminal liquid of sheep
design: Ruminal fluid of sheep was used in an in vitro experiment to messure gas production in presence of two different herbal additives, in contrast to the control; one made from seaweed Palmaria palmata and the other, containing Acacia concinna. Metagenomic DNA was extracted from each sample at 12h of incubation with ZymoBIOMICS DNA miniprep kit; microbial 16S rRNA genes targeted sequencing was performed using the Quick-16S NGS Library Prep kit (Zymo Research, Irvine, CA). The bacterial and archaeal V3-V4 region of 16S gene was amplified using Zymo's specific primers. The final library was sequenced on Illumina MiSeq with a v3 reagent kit (600 cycles) with 10% PhiX spike-in.
identifiers.namespace: SUB15059528
identifiers.id: zr12220_3V3V4