title: GSM8876013: DIPG-XH-01 cells, after radiotherapy1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRIzol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit. The libraries were sequenced on an Illumina Novaseq 6000 platform and 150 bp paired-end reads were generated. About 21.39-30.61 M raw reads for each sample were generated. Raw reads of FASTQ format were firstly processed using fastp and the low-quality reads were removed to obtain the clean reads. Then, about 19.79-26.21 M clean reads for each sample were retained for subsequent analyses. The clean reads were mapped to the reference genome using HISAT2. FPKM of each gene was calculated and the read counts of each gene were obtained by HTSeq-count.
identifiers.namespace: GEO
identifiers.id: GSM8876013_r1