title: Illumina NovaSeq 6000 paired end sequencing
library_construction_protocol: Mice were euthanized by exsanguination via cardiac puncture, and distal colon samples were collected, rinsed in PBS, and immediately placed in RNAlater (Invitrogen, Thermo Fisher Scientific, Ward Hill, MA, USA). Total RNA was extracted using the RNeasy Plus Universal Mini Kit (QIAGEN) according to the manufacturerâ€™s protocol. Poly(A)-enriched, stranded libraries were prepared using a dUTP-based protocol (fr-firststrand orientation).
identifiers.namespace: The University of Winnipeg
identifiers.id: ena-EXPERIMENT-TAB-12-11-2025-01:18:33:669-92136