title: GSM9513029: ACC4_T; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extacted from TRNzol Universal Reagent (DP424). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
identifiers.namespace: GEO
identifiers.id: GSM9513029_r1