title: GSM8664073: EU-seq of GFP-AID-Rad21 ESC, treated with 500uM IAA for 4hr, replicate3.; Mus musculus; OTHER
library_construction_protocol: After the 5-EU labeling, cells were quickly washed once with PBS, and immediately lysed in RLT buffer, and RNA was isolated using the RNeasy Mini Kit (QIAGEN) according to the manufacturer's protocol. Labeled RNA was further enriched using the Click-iT® Nascent RNA Capture Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. The labelled RNA was subsequently enriched using the Click-iT® Nascent RNA Capture Kit (Thermo Fisher Scientific) according to the manufacturer's instructions. EU-seq libraries were prepared using the NEBNext® Ultra II RNA First Strand Synthesis Module (E7771) combined with NEBNext® Ultra II Non-Directional RNA Second Strand Synthesis Module (E6111) or NEBNext® Ultra II Directional RNA Library prep kit for Illumina (E6111), following the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8664073_r1