title: Replicate 3 of bulk CUT&TAG IgG control in H1
design: Library strategy: CUT&Tag; Library selection: CUT&Tag. Third replicate of bulk CUT&Tag was performed on approximately 100,000 H1 human embryonic stem cells using IgG isotype control antibody (Millipore, 12-370) under the same protocol as the experimental samples. This included nuclei extraction, ConA bead binding, overnight antibody incubation, pA-Tn5 tagmentation, PCR amplification, and sequencing. Three biological replicates were processed in parallel as negative controls.
identifiers.namespace: SUB15377797
identifiers.id: C&TIgG3