title: Micro_O_con6
design: Total microbial community DNA was extracted using the TIANGEN DNA Isolation Kit (DP813-T8) (TIANGEN, Beijing, China) according to the manufacturers instructions. The quality of the extracted DNA was assessed by 1% agarose gel electrophoresis, and DNA concentration and purity were measured using a NanoDrop 2000 spectrophotometer.  The 16S rRNA gene V3-V4 region (the primer set and target region can be adjusted according to actual experimental needs) was amplified by PCR using the primers 338F (5-ACTCCTACGGGAGGCAGCAG-3) and 806R (5-GGACTACHVGGGTWTCTAAT-3). PCR products from the same sample were pooled and purified using a 2% agarose gel electrophoresis recovery method, followed by purification with the PCR Clean-Up Kit (MJYH, Shanghai, China). The purified PCR products were analyzed by 2% agarose gel electrophoresis and quantified using a Synergy HTX microplate reader (Biotek, USA).Library preparation was performed using the NEXTFLEX Rapid DNA-Seq Kit (Bioo Scientific, USA), and sequencing was conducted on the Illumina PE300 platform.
identifiers.namespace: SUB15200554
identifiers.id: Micro_O_con6