title: RNA-seq of Deming Lake
design: Total RNA was extracted from biomass on the 3 and 0.22 m filters using the RNeasy PowerWater Kit (Qiagen). Samples were pooled based on depth to increase the RNA yields. Total RNA was sequenced using (Novogene Co, Ltd). Samples were first passed through quality control by agarose gel electrophoresis for RNA degradation and potential contamination through DNase treatment. Nanodrop, Qubit, and Agilent 2100 were used to determine RNA purity, quantification, and integrity. RNA was fragmented and reverse-transcribed into cDNA through-end repair for tailing and adapter ligation.
identifiers.namespace: SUB15199912
identifiers.id: Deming_transcriptome_B