title: GSM8701362: Sample 4, Anti-PD1; Mus musculus; RNA-Seq
library_construction_protocol: Animals were sacrificed and livers perfused with 0.9% NaCl buffer. Liver tumors were collected, minced, digested with Collagenase and DNase, and subsequently passed through a 100μm filter. Lymphocytes were then purified by a 2-step Percoll gradient. MACS were employed for sorting live CD45+ cells. Single-cell 3' RNA (gene expression) libraries were generated according to the 'Chromium Single Cell 3' Reagents Kits User Guide (v3.1 Chemistry)' (#CG000204, 10x Genomics). Library construction was performed with Chromium Single Cell 3' Reagent Kits v3 (10x Genomics) following the manufacturer's protocol. 150bp paired-end
identifiers.namespace: GEO
identifiers.id: GSM8701362_r1