title: GSM8878632: Ercc1 d/- low carbohydrate - high protein #2; Mus musculus; RNA-Seq
library_construction_protocol: Snap-frozen liver tissues of 11-week-old Ercc1∆/− mice on diets with different macromolecular compositions were weighted to 30 mg and homogenized in QIAzol Lysis Reagent using a Qiagen tissuelyser LT. RNA was isolated using the Qiagen miRNeasy RNA isolation kit (QIAgen; 217004) following standard protocol with optional DNase (Qiagen, #79254) digestion step. RNA quality and quantity were assessed using the NanoDrop One (Thermo Fisher Scientific, ND-ONE-W) and Bioanalyzer 2100 (Agilent, Santa Clara, CA, USA; G2939BA), respectively. Samples were sent for transcriptome sequencing (GenomeScan, Leiden, The Netherlands) on the Illumina NovaSeq 6000 platform. Performed by GenomeScan using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina
identifiers.namespace: GEO
identifiers.id: GSM8878632_r1