title: GSM9278782: Tumor-LepR-single positive-4; Mus musculus; RNA-Seq
library_construction_protocol: IECs were isolated from the ileum of SFB colonized mice, and immune cells were isolated from the spleens, mLNs, and tumors and stained as described above. Fresh cells were sorted directly into RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol. RNA was extracted using the RNeasy Plus Micro Kit (Qiagen), according to the manufacturer's protocol. The quality and quantity of the extracted RNAs were assessed using Bioanalyzer RNA Pico (Agilent). mRNA pull-down and cDNA synthesis were performed using the SMART-seq v4 kit (Takara), followed by library preparation using the Nextera XT DNA Library Prep Kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM9278782_r1