title: GSM8559358: G-MDSCs, sham, POD1, rep2; Mus musculus; RNA-Seq
library_construction_protocol: G-MDSC from BALB/C mice livers were extracted at different time points (one, two, three and four days) after hepatectomy or sham operation, using magnetic-activated cell sorting (MACS). Briefly, livers were minced and incubated with digestion buffer [5% fetal bovine serum (FBS), 0.5mg/ml Collagenase VIII (Sigma Aldrich, St. Louis, Missouri; C2139) and 0.1mg/ml DNase I in PBS] for 45 min at 37°C. The cell suspension was passed through a 100µm strainer and centrifuged 3 times at 400 rpm for 5 min with the pellet discarded each time. The supernatant was then centrifuged at 1400 rpm for 5 min to obtain non-parenchymal cells. Pellets were treated with red blood cell lysis buffer and resuspended in MACS buffer (0.5% FBS, 2mM EDTA in PBS). CD11b+Ly6G+ cells were then separated sequentially using the MACS kit for G-MDSC isolation on LS and MS columns (Miltenyi Biotec) according to manufacturer's instructions. RNA was extracted using the QIAGEN RNAeasy mini-kit. Inhouse poly A based RNA seq
identifiers.namespace: GEO
identifiers.id: GSM8559358_r1