title: GSM8771544: PJH1013, Stag2-WT mouse urothelium, 1 week after TMX induction; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using a ReliaPrepTM System Kit (Promega), according to manufacturer's instructions. Total RNA samples (400ng; RQS average 9.0, range 8.7-9.3) were converted into cDNA sequencing libraries with the "QuantSeq 3' mRNA-Seq Library Prep Kit (FWD) for Illumina" (Lexogen, Cat.No. 015). Briefly, library generation is initiated by reverse transcription with oligodT priming, followed by a random-primed second strand synthesis. Primers from both steps contain Illumina-compatible sequences. Libraries were completed by PCR, and sequenced on an Illumina instrument (see below) by following manufacturer's protocols. {This library preparation kit generates directional libraries stranded in the sense orientation (the read1 (the only read in single read format) has the sense orientation (--library-type fr-secondstrand in TopHat, --stranded=yes in HTSeq)}
identifiers.namespace: GEO
identifiers.id: GSM8771544_r1