title: GSM9266860: Skin Treg_3; Mus musculus; RNA-Seq
library_construction_protocol: Where applicable, RNA was extracted using miRvana miRNeasy kit as per manufacturer's protocol. RNA sequencing libraries were constructed by Genewiz using a standard protocol in which total RNA quality is first assessed, followed by enrichment of polyadenylated mRNA using oligo(dT) magnetic beads or depletion of rRNA for total RNA applications; the resulting RNA is fragmented (typically to ~200 bp) and subjected to first- and second-strand cDNA synthesis, after which fragments are end-repaired, 3' adenylated, and ligated to Illumina-compatible indexed adapters; the adapter-ligated products are then PCR-amplified to enrich for properly ligated fragments, and the final libraries are quality controlled for size distribution and concentration before sequencing on an Illumina platform
identifiers.namespace: GEO
identifiers.id: GSM9266860_r1