title: GSM8839453: Input; Mus musculus; ChIP-Seq
library_construction_protocol: The dissected tissue was then fixed for  in 1% methanol-free formaldehyde in 1X PBS at room temperature. The crosslinking reaction was stopped by adding Glycine to a final concentration of 125mM. The tissue was washed two times lysed in SDS lysis buffer on ice subjected to sonication. The resultant chromatin was centrifuged to remove any debris. The supernatant was collected and diluted in a ChIP dilution buffer. Library preparation was performed for histone modifications  and transcription factors using TruSeq ChIP library Preparation kit
identifiers.namespace: GEO
identifiers.id: GSM8839453_r1