title: RNA-seq of Gymnodinium impudicum
design: Total RNA was extracted using the GeneAll Hybrid-R RNA Kit (GeneAll Biotechnology Co., Seoul, Korea) according to the manufacturers instructions, with genomic DNA removed via DNase I treatment (TaKaRa, Shiga, Japan). RNA quality and concentration were assessed using a Bioanalyzer RNA Pico 6000 chip (Agilent Technologies, Santa Clara, CA), measuring the RNA integrity number (RIN) and rRNA ratio. To generate a comprehensive transcriptome, RNA from all environmental conditions was pooled for sequencing. cDNA libraries were prepared using the Illumina TruSeq Stranded mRNA LT Sample Prep Kit (Illumina Inc., San Diego, CA), and sequencing was performed with 150bp paired-end reads on the Illumina NovaSeq 6000 platform (Illumina Inc., San Diego, CA).
identifiers.namespace: SUB15665965
identifiers.id: Gi_2621