title: GSM9038775: H3K27-2(ChIP-seq); Mus musculus; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis.chromatin was sonicated using a Q800R2  DNA shearing sonicator (thermocube). ChlP-seg libraries were prepared using the DNA Library Preparation Kit (nd607-01) complemented with VAHTSTM DNA adapters set2 for Illumina (N802-00) from Vazyme. 25 ng of DNA wasused as starting material for input and ip samples. Libraries were amplified using 13cycles on the thermocycler. Post amplification libraries were size selected at 250-450bpin length using VAHTS DNA clean beads from Beckman Coulter. Libraries were sequenced on Illumina Hiseq X Ten by Basepair Company (Suzhou, China).
identifiers.namespace: GEO
identifiers.id: GSM9038775_r1