title: Germline Genomic Analyses of Breast Cancer in Latinas
design: 857 genes were selected for targeted sequencing in the replication set based on discovery results and biological plausibility. A custom SureSelect XT kit (Agilent, Santa Clara, CA) was used to capture the exones of the 857 genes and 189 known BC SNPs and 100 ancestry informative SNP markers.  KAPA Hyper Preparation Kits (Kapa Biosystems, Inc, Wilmington, MA) were used to generate libraries from 500 ng DNA. One hundred base-pair paired end sequencing was performed to an average fold coverage of 75x for the targeted-sequencing samples.
identifiers.namespace: NCI-phs003144
identifiers.id: TargetedRep_BCL14840B