title: GSM8736849: Pool2_Ldb1KO_Six4KO_Zbtb7b_KO; Mus musculus; RNA-Seq
library_construction_protocol: Cells were collected on day 5 of EB culture and 1x106 mesodermal cells were FACS sorted using FLK surface expression. Isolated cells were fixed using the 10x Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit (PN-1000414) and stored at -80°C. The quantity of fixed cell suspension was assessed using an automated cell counter (LUNA-FX7, Logos). Library preparation was conducted using the 10x Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples. Briefly, four fixed samples underwent probe hybridization, pooling, and loading onto the chip. Approximately 66,000 cells from the sample pools were loaded into the 10x Chromium X. Library preparation followed the manufacturer's guidelines (Guide CG000527, Rev E).
identifiers.namespace: GEO
identifiers.id: GSM8736849_r1