title: GSM8838528: P1-G10; Mus musculus; RNA-Seq
library_construction_protocol: DIV7 neurons were harvested with Hank's Balanced Salt Solution (Gibco) supplemented with 20 Uml-1 Papain (Worthington), 5 mM MgCl2 and 5 mgmL-1 DNase I (Worthington), counted and pelleted and immediately snap-frozen in liquid nitrogen and then stored at -80°C. Nuclei extraction and fixation were performed as previously described (Cao et al. 2019), except for the use of a modified CST lysis buffer (Slyper et al. 2020) plus 1% of SUPERase In RNase Inhibitor (AM2696). Nuclei quality was checked with DAPI and Wheat Germ Agglutinin (WGA) staining. Sci-RNA-Seq3 libraries were generated as previously described (Martin et al. 2023) using three-level combinatorial indexing. The final libraries were sequenced on an Illumina NovaSeq with read cycles as follows: read 1: 34bp, read 2: 69bp, index 1: 10bp, index 2: 10bp.
identifiers.namespace: GEO
identifiers.id: GSM8838528_r1