title: GSM8770838: Patient 1 month after treatment with guideline based therapy_90; Homo sapiens; RNA-Seq
library_construction_protocol: Silica-membrane-based RNA isolation and purification in a spin-column format were performed using the PAXgene Blood RNA kit (Qiagen). Extracted RNA was globin-cleared using the Ambion GLOBINclear kit (Thermo Fisher, Waltham, MA). Then, 500 ng total RNA were rRNA-depleted using the NEBNext rRNA Depletion Kit (New England Biolabs) and converted to an Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). CD19, CD56, CD123, and CD235a antibodies was used to obtain remaining eosinophils. Total RNA from eosinophils was extracted using TRIzol™ Reagent (Invitrogen). Then, 500 ng total RNA were rRNA-depleted using the NEBNext rRNA Depletion Kit (New England Biolabs) and converted to an Illumina sequencing library using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). RNA libraries were prepared for sequencing using standard Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM8770838_r1