title: 88-8AY_S88_L001
design: To determine how our fertilization treatments influenced the bacterial community, RNA was extracted from the roots, rhizosphere and bulk soil. RNA was reverse transcribed and a PCR  was performed of the V4-V5 region of the 16S rRNA gene (515F-Y: GTGYCAGCMGCCGCGGTAA and 926R: CCGYCAATTYMTTTRAGTTT) for 16S amplicon sequencing on a MiSeq apparatus (Illumina) at the NRCC-Royalmount (Montreal, Canada) from RNA extracted from roots, rhizosphere and bulk soil.
identifiers.namespace: SUB15246771
identifiers.id: 88-8AY