title: GSM8701152: mouse corneal epithelium, 3 hours after injury, scRNA-seq, rep2; Mus musculus; RNA-Seq
library_construction_protocol: The dissected cornea tissues were pooled and incubated with 0.25% trypsin for 30 to 50 minutes at 37℃. The loose central corneal epithelial sheets were peeled and digested with fresh trypsin. Then cells were collected into RPMI medium containing 10% fetal bovine serum to deactivate and remove trypsin. Cell suspension was centrifuged, resuspended,and filtered using a cell strainer to ensure single-cell suspension. scRNA-seq library generation followed 10 ×Genomics instructions. Cells were captured in droplets. Then, reverse transcription, demulsification, Dynabeads barcode cDNA purification and PCR ampification were performed successively. The amplified cDNA was used to construct the gene expression library. scATAC-seq library generation followed 10 ×Genomics instructions. The nuclei were isolated and washed according to the manufacturer's protocal. Then resuspend the nucleus by chilled Diluted Nuclei Buffer. Nuclei were then immediately used to generate 10×single cell ATAC libraries.
identifiers.namespace: GEO
identifiers.id: GSM8701152_r1