title: Illumina NovaSeq 6000 sequencing
library_construction_protocol: As a background control for bTMP-seq, purified genomic DNA was isolated and crosslinked to bTMP with UV light. DNA was purified and 1-2ug was fragmented by sonication. 20ng DNA was kept as input and the rest of the sample was mixed with Streptavidin beads and bTMP-cross linked DNA was pulled down. The DNA was purified and Illumina library construction was carried out with the NEBNext� Ultra� II DNA library Prep kit.
identifiers.namespace: Institute for Cell Biology
identifiers.id: ena-EXPERIMENT-TAB-08-11-2025-13:02:28:923-69915