title: GSM8771665: Foxi2_MO_St9_R2; Xenopus tropicalis; RNA-Seq
library_construction_protocol: Total RNA was extracted from embryos using TRIzol reagent (ThermoFisher). RNA pellets were resuspended in DEPC-treated water and precipitated overnight at 4C in 2.5M LiCl. PolyA-selected mRNA transcripts were isolated using NEBNext PolyA mRNA Magnetic Isolation Module (NEB E7490S). RNA libraries were prepared using NEBNext Ultra II Library Preparation Kit (NEB E7770S), validated using an Agilent Bioanalyzer 2100, and sequenced on the Illumina Novaseq platform.
identifiers.namespace: GEO
identifiers.id: GSM8771665_r1