title: GSM9092495: DC1, KO, day12; Mus musculus; RNA-Seq
library_construction_protocol: Bone marrow was isolated from femur and tibia. pDC, DC1 and DC2 populations were sorted by FACS. Cells from 5 (WT or KO) mice were pooled per sample. Cells were resuspended in RLT supplemented with 2-mercapthoethanol. Total RNA was isolated with the RNAeasy mini kit (Qiagen). cDNA libraries were generated using the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara) as per manufacturers protocol.
identifiers.namespace: GEO
identifiers.id: GSM9092495_r1