title: HiSeq X Ten single Sequencing  (KAS24117031) (KAE24092736)
library_construction_protocol: Whole bodies of 2.5 days postfertilization (dpf) embryos homogenized in Trizol solution were for sequencing. Raw data were obtained from Ebiogen company. The process is summarized briefly as follows: (1) RNA QC (quality check using Bioanalyzer 2100 system); (2) cDNA library (NEBNext UltraII Directional RNA-Seq kit); (3) sequencing (Illumina HiSeq X10 paired-end 100, 4G read) (4) Trimming (Average Q20 using BBDuk); (5) mapping (Mapping using TopHat to reference genome Calculation FPKM using Cufflinks); (6) normalization (Quantile normalization using EdgeR); (7) comparison (DEG master file production using ExDEGA); (8) repor
identifiers.namespace: KOBIC
identifiers.id: KAE24092736
identifiers.namespace: KRIBB
identifiers.id: DRX857013