title: GSM9089515: Patient derived organoid treated with 1:1000 DMSO for 24 hours; Homo sapiens; RNA-Seq
library_construction_protocol: Organoid culture medium was aspirated and Matrigel was digested with trypsin at 37ºC for 10 minutes. The samples were then washed 3X with cold PBS and dissociated for 45 minutes with Accumax (StemCell cat. 07921) at room temperature to generate a single cell suspension, which was passed through a 0.45μm filter to prevent cell clumping. Samples were frozen at -80ºC in cryopreservation medium (30% organoid media with 10% DMSO and 60% FBS). Samples were then processed by GeneWiz where they underwent MACS dead cell removal and quality control Library was constructed using the 10x Genomics Chromium 3' v3 single cell RNA sequencing protocol
identifiers.namespace: GEO
identifiers.id: GSM9089515_r1