title: Bulk marine zooplankton sample
design: Illumina sequencing was performed at the KZN Research and Innovation Platform (KRISP). Libraries were purified using 1.8X AmpureXP purification beads (Beckman Coulter, High Wycombe, UK). Index PCR was performed using the Nextera XT Index Kit (Illumina, San Diego, USA). Libraries were further purified using 0.6X AmpureXP purification beads (Beckman Coulter, High Wycombe, UK) and quantified using the Qubit dsDNA High Sensitivity assay kit on a Qubit 4.0 instrument (Life Technologies, California, USA). Fragment sizes were analyzed using a LabChip GX Touch (Perkin Elmer, Hamburg, Germany) with an expected fragment size of 550 bp. Sample libraries were normalized to 4 nM concentration and denatured with 0.2 N sodium acetate. A 5% PhiX control (PhiX Control v3) was spiked in each 12 pM library and sequenced on an Illumina MiSeq platform (Illumina, San Diego, CA, USA) using a MiSeq Nano Reagent Kit v2 (500 cycles).
identifiers.namespace: SUB15378679
identifiers.id: Vertical 2