title: GSM8878056: E12p5_sample_2; Mus musculus; RNA-Seq
library_construction_protocol: Maxillary tissues of the palatal region from E12.5, E13.5, E14.5, E15.5, and E18.5 embryos (3 biological replicates per stage) were dissected and dissociated using Multi Tissue Dissociation Kit 3 (130-110-204, Miltenyi Biotec) at 37°C and 600 rpm for 15-30 minutes in a thermomixer (Thermo Fisher Scientific, 2231000269). The single-cell suspension was filtered using MACS® SmartStrainers (30 µm) (130-098-458 Miltenyi Biotec) and processed into a single-cell library using 10x Genomics Chromium single-cell 3' v3 reagent kits (10x Genomics, PN-1000269) according to the manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8878056_r1