title: snRNA-seq of mouse: KPC
design: Freshly isolated GA muscle tissues were processed for the isolation of nuclei. The nuclei were pooled from 5 mice in each group. snRNA-seq libraries were generated using ChromiumTM Single Cell 3' v3 kit (10X Genomics) according to the manufacturers protocol. Sequencing was performed on an Illumina NovaSeqXPlus system. The sample demultiplexing was performed based on 10 bp i5 and i7 sample index reads to generate Read1 and Read2 paired-end reads. The Cell Ranger Software Suite (10X Genomics, v7.0.0) was used for data demultiplexing, transcriptome alignment, and UMI counting. Raw data are stored in FASTQ (fq) format files, which contain sequences of reads and corresponding base quality. The mouse genome (GRCm38), version M23 (Ensembl 98) was used as reference for read alignments and gene counting with cellranger count. The Seurat R package was further utilized to achieve data quality control.
identifiers.namespace: SUB15638666
identifiers.id: Cachectic