title: GSM9244699: Gallbladder tumour, patient 8; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted by following the steps described in the RNeasy mini kit (Qiagen Cat No: 74104). Total RNA underwent DNASE I (Cat No: AMPD1-1KT St. Louis, Missouri, United States) digestion to eradicate genomic DNA contamination. RNA underwent a ribosomal RNA (rRNA) depletion step using the NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (Cat No: E6310S NEB Ipswich, Massachusetts, United States) as per the manufacturer's protocol. The resulting rRNA-depleted RNA was converted to double-stranded cDNA, using the Ultra RNA Library Prep kit (NEB Cat No: 7530L).
identifiers.namespace: GEO
identifiers.id: GSM9244699_r1