title: GSM8839362: 231Pa_Luc+GFP H3K27ac rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: ChIP-seq was performed using 5 μg of anti-H3K27ac antibody (#MABI009-100) or 5 ug of anti-OCT-2 antibody (#sc-377475, Santa Cruz Biotechnology, Dallas, TX, USA), as described previously (Koinuma D et al, Mol cell biol, 2009). The libraries were constructed as described (Murai et al, Cell Discov, 2015), using IonXpress Plus Fragment Library Kit (Thermo Fisher Scientific). Adaptor-ligated samples were amplified by 15 cycles of PCR and purified by E-Gel SizeSelect (Thermo Fisher Scientific).
identifiers.namespace: GEO
identifiers.id: GSM8839362_r1