title: dsRNA Nanopore sequencing- Asymptomatic Lavandula latifolia 01
design: dsRNA was extracted from asymptomatic leaf, petioles and shoot tissues of Lavandula angustifolia, Lavandula latifolia, and Lavandula  intermedia using a cellulose-based protocol. Double-stranded cDNA was synthesized using Maxima H Minus reverse transcriptase and Klenow polymerase. Library prep followed the ONT Ligation Sequencing with Native Barcoding Kit V14 (SQK-NBD114.96), and sequencing was performed using a MinION R10.4 flow cell. The dataset supports metagenomic detection of RNA viruses in lavender.
identifiers.namespace: SUB15245568
identifiers.id: dsRNA_L1