title: 10275-JF-13
design: Generation of single cell suspensions for single-cell RNA-sequencing (scRNA-seq) was performed as follows: Samples were incubated overnight in 0.4% dispase (Life Technologies) in Hanks Balanced Saline Solution (Gibco) at 4C. Epidermis and dermis were separated. Epidermis was digested in 0.25% Trypsin-EDTA (Gibco) with 10U/mL DNase I (Thermo Scientific) for 1 hour at 37C, quenched with FBS (Atlanta Biologicals), and strained through a 70?M mesh. Dermis was minced, digested in 0.2% Collagenase II (Life Technologies) and 0.2% Collagenase V (Sigma) in plain medium for 1.5 hours at 37C, and strained through a 70?M mesh. Epidermal and dermal cells were recombined and libraries were generated using the 10X Chromium platform.
identifiers.namespace: SUB14845047
identifiers.id: 10275-JF-13