title: Vec_2 (CRX704213; CRA011055)
library_construction_protocol: Total RNA was extracted using Trizol reagent kit (Invitrogen, Carlsbad, CA,USA) protocol. After total RNA was extracted, eukaryotic mRNA was enriched, fragmented and reverse transcripted into cDNA. Then the cDNA fragments were purified with QiaQuick PCR extraction kit(Qiagen, Venlo, The Netherlands), end repaired, poly(A) added, and ligated to Illumina sequencing adapters.Proximal mouse tubular epithelial cells were infected with an adenovirus construct containing an empty vector.
identifiers.namespace: NGDC
identifiers.id: CRX704213
identifiers.namespace: Nanfang Hospital, Southern Medical University
identifiers.id: DRX710204