title: RNA-seq of whole Phaseolus vulgaris seed: TZ-27, soaked for 12 hours, stored for 3 days at 25C/50% RH
design: Total RNA was extracted from whole seed material using two Millipore Sigma Spectrum Plant Total RNA (STRN50) kits according to manufacturers instructions. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation kit with IDT for Illumina RNA UD Indexes, following manufacturers recommendations except that half volume reactions were performed. The quality and quantity of the completed libraries was determined using a Qubit dsDNA HS and an Agilent 4200 TapeStation HS DNA1000. The libraries were pooled in equimolar amounts, and the pool was quantified using the Invitrogen Collibri Quantification qPCR kit. The pool was loaded onto one lane of an Illumina S4 flow cell and sequencing was performed in a 2x150bp paired end format using a NovaSeq v1.5, 300 cycle reagent kit. Base calling was done using Illumina Real Time Analysis (RTA) v3.4.4. The RTA was demultiplexed and converted to FastQ format with Illumina Bcl2fastq v2.20.0.
identifiers.namespace: SUB14654548
identifiers.id: M2