title: PRBR00384U02 (CRX1267012; CRA020515)
library_construction_protocol: DNA was extracted from on average 4 ml of urine per sample using the DNeasy 96 Blood & Tissue kit. PCR was done with the forward primer S-D-Bact-0341-b-S-17 and reverse primer S-D-Bact-0785a-A-21 (Klindworth et al. 2013) with Illumina adapters attached. Products from the nested PCR were pooled based on band intensity and the resulting library cleaned with magnetic beads. The DNA concentration of pooled libraries was measured fluorometrically. Sequencing was done on an Illumina MiSeq desktop sequencer using the MiSeq Reagent Kit V3 (Illumina) for 2x 300 bp paired-end sequencing.
identifiers.namespace: NGDC
identifiers.id: CRX1267012
identifiers.namespace: Queen Mary University of London
identifiers.id: DRX720294