title: GSM8839861: G2: 50 µg QS-21 SMNP at week 12 GEX; Macaca mulatta; RNA-Seq
library_construction_protocol: Env-binding memory B cells at week 12 from 50 µg and 400 µg QS-21 SMNP groups were stained with tetramerized BG505 MD39 probes using fluorescent-SA and TotalSeq-C oligo-tagged fluorescent-SA, surface antibodies and six different TotalSeq-C oligo-tagged anti-b2M & CD298 antibodies to multiplex animal samples. Samples were sorted on a FACSymphony S6 (BD Biosciences) into 1.5 mL tubes with FBS supplemented RPMI media. Libraries were constructed according to the manufacturer's protocol using the Chromium Next GEM Single Cell 5' Reagent Kit v2.
identifiers.namespace: GEO
identifiers.id: GSM8839861_r1