title: GSM8615003: Kelly-shRUNX1T1_NT_LSD1_Rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixes with 1% formaldehyde for 10 min. After quenching, chromaitin was fragmented through sonication. Chromatin samples were immunoprecipitated overnight at 4°C. The complexes were immunoprecipitated with antibodies specific for RCoR1 (#EPR13825-ABCAM), RCoR3 (#ab76921-ABCAM), FLAG (#F3165-Sigma Aldrich) and LSD1 (#ab129195-ABCAM). The antibody/protein complexes were collected by Sepharose rec-Protein A or G (#101042 Invitrogen) and washed several times. The immune complexes were eluted with 1% SDS and 0.1 M NaHCO3 and samples were treated with proteinase K for 6h and DNA was purified by phenol/chloroform extraction, ethanol precipitation, and resuspended in H2O. DNA libraries for ChIP-seq were prepared using Kapa Hyper Prep Library Prep Kit following manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8615003_r1