title: RNA-seq of Malus domestica : axillary shoot
design: Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dTTP for non strand specific library or dUTP for strand specific library. The process was completed after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.
identifiers.namespace: SUB15756429
identifiers.id: BA_1