title: GSM8740376: Intermediate mesoderm (IM) cells - IM CTL; Homo sapiens; RNA-Seq
library_construction_protocol: Single-cell suspensions were made from human kidney organoid using TrypLE (Thermo Fisher, 12604013), and then filtered through a 40 uM mesh strainer (Corning, CLS431750) to isolate single cells. Single-cell RNA-sequencing libraries were then prepared using the Chromium Next GEM Single Cell 3' reagent kit v3.1 (10X Genomics, Pleasanton, CA) in accordance with the manufacturer's protocol. libraries were sequenced in multiplex on the Novaseq 6000 sequencer (Illumina, San Diego, CA) to produce on average a minimum of 60,000 reads per single cell
identifiers.namespace: GEO
identifiers.id: GSM8740376_r1