title: GSM8839473: hlh-30(tm1978), 48 hours ARD, replicat 4; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: For RNA sequencing, total RNA was prepared from at least 3000 worms per genotype, using the RNeasy Mini Kit (QIAGEN) polyA+ mRNA was isolated using NEBNext Poly(A) mRNA Magnetics Isolation Module (New England Biolabs), RNA-seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs), quantified by fluorometry, immobilized and processed onto a flow cell with a cBot (Illumina) followed by sequencing-by-synthesis with TruSeq v3 chemistry on a HiSeq2500 at the Max Planck Genome Center (Cologne, Germany).
identifiers.namespace: GEO
identifiers.id: GSM8839473_r1