title: Asynchronous viral spread of two unrelated viruses determines Lettuce Big Vein Disease symptom development
design: Material was grinded using mortar and pestle to a fine powder and used as input material for total RNA isolation using the RNeasy Plant Mini Kit (Qiagen). The isolated total RNA was subjected to ribosomal depletion using the RiboMinus Plant Kit for RNA-Seq (Thermo Scientific). The remaining RNA was used for cDNA synthesis using the Maxima H Minus Double Stranded cDNA Synthesis Kit using random hexamer primers (Thermo Scientific). Oxford Nanopore library construction was performed with ds-cDNA as input. For library construction, the Native Barcoding Kits SQK-NBD114.96 (Oxford Nanopore) was used. Manufacturers instructions and protocols were followed. The constructed sequencing libraries were sequenced using the Promethion FLO-PRO114M flowcells (Oxford Nanopore) during a period of 72 hours.
identifiers.namespace: SUB15004863
identifiers.id: phy_lID0220_Barcode24