title: GSM8584356: Chimp 89008 day 141; Pan troglodytes; OTHER
library_construction_protocol: Total mRNA was extracted from tissues via the NucleoSpin RNA kit from Takara Biosciences using the manufacturers recommended protocol Iso-seq library was prepared according to PacBio's standard protocol. Briefly, cDNA was synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech) and was PCR-amplified. Following Blue Pippin size selection to create four size bins (<1kb, 1-2kb, 2-3kb, and 3-6kb), the cDNA was further amplified with PCR, ligated to hairpin adapters to create the SMRTbell Template, annealed to sequencing primer, and bound to polymerase for sequencing on the RSII sequencer by generation of concatemers. For chimpanzee 88A010, the size bins were 1-2kb, 2-3kb, 3-6kb and >6kb.
identifiers.namespace: GEO
identifiers.id: GSM8584356_r1