title: DNA-seq of Propionibacterium freudenreichii: L-lactate negative strain
design: Libraries were prepared according to the manufacturers instructions using the Ion Xpress Plus gDNA Fragment Library Preparation kit (Thermo Fisher Scientific, Waltham, MA, USA). Briefly, 1 ug DNA was sheared during 8 minutes to obtain 400 bp fragments. Adapters with barcodes were ligated to the fragments. Size selection was performed with E-Gel SizeSelect Agarose Gel (Thermo Fisher Scientific). Libraries were then quantified by qPCR with the Ion Library Quantitation Kit (Thermo Fisher Scientific) using a Corbett Rotor-Gene 3000 (Qiagen, Hilden, Germany). No library amplification was performed.
identifiers.namespace: SUB15163875
identifiers.id: R_2021_03_05_02_56_58_user_S5-0413-48-Gaerungsorganismen_CHEF_530-shtgn-1_IonCode_0102.fastq