title: RNA-seq of HSPA8 knockdown in MDA-MB-468 cells
design: RNA-seq of HSPA8 knockdown in MDA-MB-468 cells
library_construction_protocol: Cells transfected with siRNAs with Lipofectamin as the transfection reagent. Cells were cultured at 37Â°C in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and 100 IU/mL penicillin-streptomycin solution under 5% CO2. 48-hours post-transfection, total RNA was extracted using TRIzol reagent (Ambion). VAHTS Universal V8 RNA-seq Library Prep Kit (Vazyme) was used to prepare strand-specific RNA-seq libraries.
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-16073:NC_1_p