title: GSM8877615: Paraxial_Mesoderm_and_PS pool; Mus musculus; RNA-Seq
library_construction_protocol: Cells at indicated stages were dissociated using Accutase to single-cell suspension in PBS-/- with 0.04% BSA. Samples were labeled with Cell Multiplexing Oligos (10x Genomics Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 Dual Index) and live cells sorted using DAPI on a BD Influx Cell Sorter. Three pools were created for multiplexing in PBS-/- with 0.04% BSA (targeting 10,000 cells/sample)- EpiSCs, DE with DMSO or dTAG13 added at PS stage; Paraxial Mesoderm with DMSO or dTAG13 added at PS stage; and DE with DMSO or dTAG13 added at 24hrs DE differentiation. Single-cell libraries were generated according to the manufacturer's instructions (10x Genomics Chromium Single Cell Gene Expression - Cell Multiplexing).
identifiers.namespace: GEO
identifiers.id: GSM8877615_r1