title: GSM8839176: Mock infected mouse 1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from lung tissue homogenates from day 2 post infection using TRIzol (Invitrogen). Following separation with 1-bromo-3-chloropropane, aqueous phase was collected, and RNA was precipitated with ethanol. Precipitated RNA was isolated and sent to GENEWIZ, LLC/Azenta US, Inc (South Plainfield, NJ, USA). RNA library preparation and sequencing was performed with the following methods as provided by GENEWIZ: “The RNA samples received were quantified using Qubit 2.0 Fluorometer (ThermoFisher Scientific, Waltham, MA, USA) and RNA integrity was checked using TapeStation (Agilent Technologies, Palo Alto, CA, USA). The RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (New England Biolabs, Ipswich, MA, USA).
identifiers.namespace: GEO
identifiers.id: GSM8839176_r1