title: GSM9188610: HuCCT1_Veh_bio rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using Quick-RNA Miniprep Kit (Zymo Research, R1055) with on-column DNase I digestion to eliminate genomic DNA contamination. RNA integrity was verified by Agilent 2100 Bioanalyzer (RIN > 8.0), and concentrations were quantified via Qubit 4.0 Fluorometer (Thermo Fisher Scientific). For bulk RNA sequencing, 1 μg of total RNA per sample underwent library construction. Libraries were sequenced on an Illumina NovaSeq 6000 platform (150-bp paired-end, 30M reads/sample).
identifiers.namespace: GEO
identifiers.id: GSM9188610_r1