title: GSM9191693: RNA-seq of E407nm.1; Mus musculus; RNA-Seq
library_construction_protocol: RNA was extracted from CD11b⁺CD169⁺CD11c⁺ cells using the RNeasy Micro Kit (QIAGEN) and processed for library preparation (TruePrep DNA Library Prep Kit; Vazyme). Sequencing was performed on the DNBSEQ-G400 platform (BGI) generating 100 bp paired-end reads. Raw data underwent quality control with SOAPnuke v1.5.6 to remove: 1) adapter-containing reads, 2) reads with >0.1% unknown bases, and 3) reads with >20% low-quality bases (Q≤15). Clean reads were aligned to GRCm39 using HISAT2 v2.2, then to reference gene sets with Bowtie2 v2.4.5.
identifiers.namespace: GEO
identifiers.id: GSM9191693_r1