title: DNA sequencing of PbCas-DiCre-GFP parasites
design: Two pools of 6 libraries were prepared from at least 400 ng of gDNA from each parasite strain using the SQK-NBD114-24 ligation kit following the supplier's protocol. The gDNAs from one PbCas9, two PbCasDiCre-GFP, two PbCasDiCre-mCherry and one clampcKO sample were multiplexed in each pool. The first pool was sequenced on a MinION R10.4.1 flow cell (Cat#FLO-MIN114) for 30h, then the flow cell was washed with the kit (WSH004) following the manufacturer's protocol and the second pool was sequenced for 66h on the same flow cell. Fast5 raw data was generated using ONT's MinKnow v 23.04.5 software and demultiplexing was performed in real time using guppy software (version 6.5.7).
identifiers.namespace: SUB14836793
identifiers.id: malaria03_3