title: GSM8877714: 22719X53 13 Pre Old; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA extraction for PBMC samples was achieved using RNeasy Plus Mini Kit (Cat No: 74134; Qiagen), with DNase treatment. Total RNA samples (5-500ng) were hybridized with NEBNext rRNA Depletion Kit v2 (Human, Mouse, Rat) (E7400) to substantially diminish rRNA from the samples. A total amount of 200ng RNA per sample was used as input material for the RNA-seq sample preparations. Stranded RNA sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L).
identifiers.namespace: GEO
identifiers.id: GSM8877714_r1