title: IT-scC&T-seq of H3K4me3 and H3K27ac in 10-week female mouse mammary gland, replicate 2
design: Library Strategy: CUT&Tag. Library Selection: CUT&Tag. Approximately 1,000,000 fixed nuclei were extracted from adult mouse mammary gland tissues (biological replicate 2) and incubated with H3K4me3 or H3K27ac antibodies. Following secondary antibody incubation, indexed pA-Tn5 complexes were used for tagmentation in bulk. Six pA-Tn5 index pairs per mark corresponding to respective histone mark were applied across replicates. Tagmented nuclei were pooled and single nuclei were sorted into three 384-well plates. Two rounds of PCR introduced well- and plate-specific indexes. Libraries were pooled, purified, and quality-controlled before Illumina sequencing.
identifiers.namespace: SUB15374457
identifiers.id: MmMGscCT2