title: S4_1-metavirome
design: Metagenomic libraries were prepared following TruSeqTM Nano DNA sample preparation Kit from Illumina (San Diego, CA), using 1ug of total DNA. DNA end repair,A-base addition and ligation of the Illumina-indexed adaptors were performed according to Illuminas protocol. Libraries were then size selected for DNA target fragments of ~400bp on 2% Low Range Ultra  Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles. All samples were sequenced by the Illumina NovaSeq 6000 platform (150bp*32, Guangdong Magigene Biotechnology Co., Ltd, Guangdong, China)
identifiers.namespace: SUB13701310
identifiers.id: S4_1v