title: RNA-Seq of human glioblastoma cell line U118-MG
design: For RNA, cells were harvested in Trizol according to the manufacturers instrutions. After homogenization, samples were stored at -80C until further processing. RNA was isolate using the Purelink RNA mini kit with on column Dnase I treatment. RNA integrity was evaluated using RNA tapestation. Stranded mRNA-seq libraries were constructed using the Illumina stranded mRNA seq kit according to the manufacturers instructions
identifiers.namespace: SUB16000552
identifiers.id: U118_LIN_10uM_72h_rep3