title: XTGY
design: n terms of RNA extraction and transcriptome sequencing for the purpose of facilitating genome annotation, total RNA was isolated from multiple tissues including brain, liver, kidney, heart, muscle, spleen, and skin. The quality of the extracted RNA was evaluated using both the NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA) and the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Following the manufacturer's protocols, cDNA libraries were constructed from the pooled RNA samples using the MGIEasy Universal DNA Library Preparation Kit (MGI, China). These libraries were then sequenced on the MGISEQ T7 platform (MGI, China) with a paired-end 150 bp sequencing layout.
identifiers.namespace: SUB15439008
identifiers.id: XTGY-RNA