title: GSM8902943: KO.Tx_793; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using phenol/chloroform. 200 nanograms total RNA was used for the construction of sequencing libraries. Library prep and sequencing were done at Innomics, Inc. Oligo dT beads were used  to enrich mRNA with poly A tail, then mRNA molecules were fragmented into small pieces. The fragmented mRNA was synthesized into first strand cDNA using random primers. The second strand cDNA was synthesized with dUTP instead of dTTP. The synthesized cDNA was subjected to end-repair and 3' adenylated. Adaptors were ligated to the ends of these 3' adenylated cDNA fragments, then the fragments were PCR amplified. After quality control,  Libraries were circularized, then amplified to make DNA nanoball (DNB).
identifiers.namespace: GEO
identifiers.id: GSM8902943_r1