title: GSM8806401: CRISPR-YScr Replicate 2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) with gDNA Eliminator columns, following the manufacturer's protocol. RNA integrity was assessed using Agilent Bioanalyzer 2100. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit following the manufacturer's instructions. mRNA was enriched using poly-A selection, fragmented, and reverse-transcribed into cDNA. Libraries were indexed and amplified by PCR, followed by quality control and quantification.
identifiers.namespace: GEO
identifiers.id: GSM8806401_r1