title: GSM8840237: lung, Influenza A Virus, S4_GEX; Mus musculus; RNA-Seq
library_construction_protocol: Animals were euthanized by administration of a lethal dose of ketamine/xylazine mixture and perfused with 2mM EDTA in PBS. The trachea was exposed, nicked, and a 22g catheter was inserted. A 1mL-PBS-filled syringe was attached to the catheter and used to inflate then deflate lungs to collect cells present in BALF. The syringe was replaced with a 1mL-dispase-filled syringe and lungs were inflated. Inflated lungs were removed, and lung lobes were chopped and immersed in enzyme solution (100μg/mL DNAse and 83μg/mL Liberase in RPMI) and placed in a 37°C shaker for 40 minutes. BALF and lung digest cells were filtered through a 70μm cell strainer to obtain a single cell suspension and exposed to hypotonic lysis (ACK lysing buffer, ThermoFisher) to remove red blood cells. Samples were prepared using a Chromium Next GEM Single Cell 3' Kit v3.1 according to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8840237_r1