title: GSM8877423: S0037 Single cell RNAseq on OS-17 tibial tumor PDX; Homo sapiens; Mus musculus; RNA-Seq
library_construction_protocol: Lungs harvested from mice were processed using the human tumor dissociation kit (Miltenyi Biotec, 130-095-929) with a GentleMacs Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427). HBEC mono-cultures, OS-17 monocultures, or HBEC:OS-17 co-cultures (72 hours) were gently trypsinized and dissociated into single cell suspensions. Following dissociation into single cell suspension, single-cell 3' RNA-Seq libraries were prepared using Chromium Single Cell 3′RNA-sequencing system (10X Genomics) with the Reagent Kit v3.1 (10X Genomics, PN-1000121) according to the manufacturer's instructions. cDNA library quality and quantity were determined using an Agilent 2100 bioanalyzer using the High Sensitivity DNA Reagents kit (Agilent, 5607-4626) and then sequenced.
identifiers.namespace: GEO
identifiers.id: GSM8877423_r1