title: GSM8877902: HBEC ATAC, WTSal1; Homo sapiens; ATAC-seq
library_construction_protocol: DNA was extracted using the Zymo DNA Clean and Concentrator-5 Kit ATAC-seq libraries were prepared for a total of 12 samples (initial n = 3 for WT saline, n = 3 for WT HDM, n = 3 for KD saline, HDM, n = 3 for KD HDM). Samples were prepared according to the Omni-ATAC-seq protocol. Briefly, 50,000 viable cells were lysed and nuclei were extracted. Nuclei were then treated with transposase at 37°C for 30 minutes (Nx#-tde1, tagment DNA enzyme, Illumina). DNA was extracted using (Zymo DNA Clean and Concentrator-5 Kit) and libraries were prepared. Preamplification with 5 cycles was performed and qPCR was used to determine additional PCR cycles. Libraries were then cleaned up and their quality was determined by bioanalyzer. Indexed libraries were then mixed and sequenced at the DNA technologies core at UC Davis across two sequencing runs
identifiers.namespace: GEO
identifiers.id: GSM8877902_r1