title: ITS2 sequencing of Galaxea fascicularis Symbiodiniaceae  community
design: DNA was extracted from adult coral samples. The extraction was conducted with bead beating and additional enzymatic digestion steps with Proteinase K. Symbiodiniaceae ITS2 primers SYM_VAR_5.8S2 (forward: 5-GTGACCTATGAACTCAGGAGTCGAATTGCAGAACTCCGTGAACC-3) and SYM_VAR_REV (reverse: 5-CTGAGACTTGCACATCGCAGCCGGGTTCWCTTGTYTGACTTCATGC-3) (Hume et al., 2018) with Illumina adapter sequences (underlined) added at the 5-prime ends were used to amplify the ITS2 molecular marker.
identifiers.namespace: SUB15802242
identifiers.id: gal_sample_99