title: GSM8458437: BALF, wt-sham; Mus musculus; RNA-Seq
library_construction_protocol: BALF samples were collected from both WT and Padi2-/-Padi4-/- mice after PA infection and sham control. BALF samples from three mice per group were pooled to generate samples (n =3 mice/sample) for the isolation of BALF cells. The BALF samples were centrifuged for 5 minutes at 400 x g and 4°C. The cell pellet was then resuspended in 500 µL of red blood cell lysis buffer and incubated for 5 minutes at room temperature. Following this, 500 µL of cold PBS was added to dilute the RBC lysis buffer, and the mixture was centrifuged for 5 minutes at 400 x g and 4°C. The resulting single cells were resuspended in PBS containing 1% weight/volume FBS. RNA libraries were prepared with  the Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit (10x Genomics) according to the manufacture's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8458437_r1