title: Santa Fe Rainy nifH19
design: For this library we prepared a PCR mix containing 13.17ul H2O, 5.08ul of MM KAPA, 0.5ul of BSA, 1ul of F Primer, 1ul of R Primer, 1.25 of Magnesio and 3ul of DNA. The first PCR was: Read primer Adapter + Locus specific primers. Second PCR contained Illumina flow cell adapter + index + read primer adapter. Then we concentrate/wash the library with Serapure beads (1x vol beads).  After that we check  the library concentrations with the Nanodrop and/or Qubit. Finally, we check the library quality with the BioAnalyzer High Sensitivity Kit.
identifiers.namespace: SUB15309487
identifiers.id: nifH_OSF_19