title: Lambda Chain BCR-seq of mustela putorius furo: female splenocytes
design: cDNA isolation: RNA was isolated from bulk cell samples using the RNeasy Mini Kit (Qiagen, 74104), and cDNA was synthesized using SuperScript IV (Invitrogen, 18091050), both per the manufacturers instructions. Priming strategy: multiplexed forward primer set from Wong...Wheatley 2020 targeting IGLV leader2 sequences and single lambda-CH1-specific reverse primer (targeting a sequence shared among reported functional lambda genes). Sequencing: performed by Genomic Sequencing and Analysis Facility at UT Austin, Center for Biomedical Research Support (RRID# SCR_021713) using the Illumina MiSeq 2 x 300 paired-end platform (MiSeq Reagent Kit v3, 600 cycles, MS-102-3003).
identifiers.namespace: SUB15274130
identifiers.id: V-L_F1