title: GSM9038688: Mycn-nGEMM-low tumor and CD45 cells, biol rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Mycn-nGEMM tumors were harvested and were dissociated into single cells. Tumor cells were digested with collagenase I, collagenase IV and deoxyribonuclease I at 37C under agitation for 30min. Cell suspensions were strained through a 70um mesh cell strainer and washed with PBS. ACK lysing buffer were added to lyse erythrocytes for 5min at room temperature. Reactions were quenched with PBS. Cells were stained by LIVE/DEAD cell stain kit for 30min and by anti-mouse CD45 antibody for 30min. Finally, live tumor and CD45+ cells were isolated by FACS for scRNA-seq. Library was performed according to the manufacter's instructions (single cell 3' Gene Expression v3.1 protocol, 10X Genomics) by MedGenome.
identifiers.namespace: GEO
identifiers.id: GSM9038688_r1