title: GSM8839494: FA19, Gen 0, Rep 1; Neisseria gonorrhoeae; RNA-Seq
library_construction_protocol: RNA was harvested using the Rneasy Mini Plus Kit (Qiagen). Quality of the total RNA was assessed using the Agilent 2100 Bioanalyzer The Agilent SureSelect Strand Specific mRNA library kit as per the manufacturer's instructions (Agilent, Santa Clara, CA).  Library construction began with ribosome reduction using the RiboMinus rRNA depletion kit for bacteria as described by the manufacturer (Invitrogen).  The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems, Woburn, MA) prior to cluster generation.  Cluster generation was performed according to the manufacturer's recommendations for onboard clustering (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM8839494_r1