title: GSM8557679: H3K27ac antibody, B6, Normal  chow fed, ZT10; Mus musculus; OTHER
library_construction_protocol: ChIA-PET experiments were performed as described (Zhu et al. 2023) with minor changes. In briefly,  mouse livers were harvested and cross-linked in 1% formaldehyde for 20min, followed by quenching with 1/20 volume of 2.5M glycine solution for 5 min, and two washes with 1×PBS. Nuclear extracts were prepared by Dounce homogenization. 30 ml AluI enzyme were used to digested genome of dual-crosslinked liver nuclei at 37℃ O/N. Exposed ends of genomic DNA were dA-tailed and ligated with dT-overhang biotinylated bridge linker at 16℃ O/N. The ligated chromatin was sheared by sonication and pull-down by H3K27ac antibody. Immunoprecipied DNA was subjected to Tn5 tagmentation, M280 Dynabeads streptavidin pull-down and PCR amplification. PCR products were subjected to size-selection and pairend sequencing on Illumina Hiseq or Novaseq platform.
identifiers.namespace: GEO
identifiers.id: GSM8557679_r1