title: GSM8740357: SUM159-1-3, HIF-1α, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, chromatin was sonicated and the DNA was sheared to an average length of 300-500 bp with Covaris E220 sonicator. DNA libraries were prepared using the Takara Bio Low Input ChIP-seq kit according to manufacturer's instructions. 1 ug of ChIP DNA was used from each sample and amplified with 16 PCR cycles. Libraries were sequenced at an average depth of 50 million reads using an Illumina NovaSeq sequencer (2 × 100 reads)
identifiers.namespace: GEO
identifiers.id: GSM8740357_r1