title: RNA_Seg of osteoclast 2
design: Femurs and tibias of 6 to 8 weeks old C57BL/6 mice were aseptically removed, and bone marrow macrophages (BMMs) were flushed out of the marrow cavity with -MEM. Cells were cultured in -MEM containing 10% FBS and 20 ng/ml M-CSF at 37 C and 5% CO2. M-CSF-dependent BMMs were seeded in 6-well plates at a density of 1.25106 cells per well in complete -MEM supplemented with or without 50 ng/ml RANKL. The medium was refreshed every other day. After 9 d of induction, the cells were washed by PBS twice and total RNA was extracted using TRIzol for transcriptome sequencing.
identifiers.namespace: SUB15608669
identifiers.id: M+R_2