title: GSM8584254: E7.75, FLAG-Lhx1, rep 2; Mus musculus; RNA-Seq
library_construction_protocol: Geo-seq protocol was used to generated mRNA libraries (“Spatial transcriptomic analysis of cryosectioned tissue samples with Geo-seq”, 2017, Nature Protocol, 12:3, 566-580). 10 cycles of cDNA preamplification used after optimisation. 0.65x AMPure XP beads used after cDNA pre-amplification. 0.5 ng cDNA used for library preparation with the Nextera XT DNA kit. After library preparation, 0.5x AMPure XP beads followed by 0.15x AMPure XP beads were used.
identifiers.namespace: GEO
identifiers.id: GSM8584254_r1