title: HL_2_INPUT (CRX712351; CRA011232)
library_construction_protocol: Total RNA was extracted using TRIZOL reagent following the manufacturer's procedure. The mRNA was  isolated from approximately 50 ug total RNA using magnetic beads with poly-T oligonucleotides, then chemically fragmented 150nt-long oligonucleotides.Tthe cleaved RNA fragments were subjected to incubated for 2h with m6A-specific antibody in IP buffer supplemented with BSA. The mixture was then incubated with protein A beads and eluted with elution buffer. Eluted m6A-containing fragments and untreated input control fragments are converted to final cDNA library in accordance with a strand-specific library preparation by dUTP method. submitted_library_strategy: MeRIP-Seq
identifiers.namespace: Fudan University Eye Ear Nose and Throat Hospital
identifiers.id: DRX712907
identifiers.namespace: NGDC
identifiers.id: CRX712351