title: GSM9278737: TM+ Rep3, INPUT; Mus musculus; ChIP-Seq
library_construction_protocol: Cells were cross-linked with 1% formaldehyde (Fisher Bioreagents, BP531-500) for 7 minutes at room temperature, and the reaction was quenched by adding glycine (Fisher Scientific, BP381-1) to a final concentration of 0.125 M. The bioChIP protocol was conducted as previously described (Kim et al, 2009). Genomic DNA was sheared to an average fragment size of ~200 bp using a Bioruptor sonicator (Diagenode). Cross-linked chromatin was pre-cleared with Protein A agarose beads (Thermofisher, 10008D) and subsequently subjected to immunoprecipitation using Dynabeads MyOne Streptavidin T1 (Invitrogen, 65602). Library preparation was performed using the NEBNext® Ultra™ DNA Library Prep Kit (NEB, 7103L)
identifiers.namespace: GEO
identifiers.id: GSM9278737_r1