title: RNA-seq of mus musculus: vagal ganglia
design: Messenger RNA was purified from total RNA using poly-T oligo attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexer primers, followed by the second strand cDNA synthesis using dUTP. Library was then end repaired, A-tailed, adapter ligated, enzyme digested to remove UTP-containing second strand cDNA, PCR amplified and size selected.
identifiers.namespace: SUB14768166
identifiers.id: Con_F2