title: GSM8770531: Mouse primary neuron, PXN KD-2, DIV10, rep4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted utilizing the RNeasy Mini Kit (Qiagen; Cat# 74104) and its concentration was determined using a Nanodrop 2000 spectrophotometer and a Qubit fluorometer (both from ThermoFisher Scientific). The quality of the extracted RNA was evaluated using a 2100 Bioanalyzer (Agilent). 1ug of total RNA per sample was subjected to library preparation using SureSelect XT HS2 mRNA Library Prep Kit (Agilent Technology, USA) on the Agilent Bravo Automated Liquid Handling Platform. Libraries were sequenced using NovaSeq6000 (Illumina) with paired-end 150. 60 million reads per sample was obtained and subjected to RNA-seq analysis.
identifiers.namespace: GEO
identifiers.id: GSM8770531_r1