title: 16h triptolide incubation replicate 2
design: MOLM-13 cells were cultured and prepared for drug treatment under optimal conditions in RPMI medium supplemented with 10% fetal bovine serum (FBS) at a density of 0.5 x 10^6 cells per mL. For half-life determination with transcriptional inhibition treatment, cells were resuspended in fresh RPMI with 10% FBS and treated with 1 M triptolide (Abcam, ab120720). Cells were washed twice with PBS and collected for RNA extraction at various time points post-treatment (0, 0.5, 1, 2, 4, 8, and 16 hours). Three biological replicates were collected per time point. RNA extraction was performed using the Total RNA Purification Kit (Norgen, SKU 48500). Libraries were prepared by polyA selection and sequenced on the Novaseq X Plus platform
identifiers.namespace: SUB15075630
identifiers.id: A20