title: scRNA-seq of homo sapiens: post MMR vaccination
design: Sorted B cells in the memory B cell gates were processed for single cell RNAseq using the Chromium Next GEM Single cell 3 Reagent kits v3.1 (10X Genomics). 10X cDNA was enriched with an xGen Custom Hybridization Panel targeting BCR and TCR exons, PCR amplified and then prepared using the Kit14 Ligation Sequencing Kit (Oxford Nanopore Technologies, SQK-LSK114) and sequenced on R10 E8.2 flow cells. Basecalled with dna_r10.4.1_e8.2_400bps_sup@v5.0.0.
identifiers.namespace: SUB14769163
identifiers.id: PAW28803_MMR_P8_R10