title: Sequence of HCV isolate QC757_GT 5
design: cDNA was generated using an oligo dA primer (not oligo dT). A TSO containing a randomized 8 bp UMI=N8 (TSO Sequence: TGAGACATCTAGCACATGTNNNNNNNNWWrGrGrG) was used to append a 5' primer sequence and Nextera XT Tn5 recognition sequence. Long PCR amplification was performed followed by Nextera XT (Illumina) processing to attach dual unique indexes and size the library to ~250 bp.
identifiers.namespace: SUB14908942
identifiers.id: QC575_H22-5027