title: Ken Yu 90
design: After the samples pass the quality inspection, library construction is carried out. The main steps are as follows:(1) Enrich eukaryotic mRNA using magnetic beads conjugated with Oligo (dT);(2) Add fragmentation buffer to randomly fragment the mRNA;(3) Synthesize the first cDNA strand using the mRNA as a template and random hexamers as primers. Then, add buffer, dNTPs, RNase H, and DNA polymerase I to synthesize the second cDNA strand, and purify the cDNA with AMPure XP beads;(4) Perform end repair, A-tailing, and sequencing adapter ligation on the purified double-stranded cDNA. Subsequently, use AMPure XP beads for fragment size selection;(5) Obtain the cDNA library through PCR enrichment.
identifiers.namespace: SUB15710804
identifiers.id: 812B2