title: Rhizosphere soil microbiome of Newhall navel orange
design: Genomic DNA was extracted from rhizosphere soil using the MoBio PowerSoil DNA Isolation Kit. The V4 hypervariable region of the bacterial 16S rRNA gene was amplified with barcoded primers 515F/806R and Phusion High-Fidelity Master Mix. Libraries were constructed from purified amplicons and SINGLE-end sequenced (2x250 bp) on an Illumina MiSeq platform. The submitted data represent the final effective tags: raw SINGLE-end reads were demultiplexed, merged (FLASH v1.2.11), quality-filtered (fastp v0.23.1), and chimera-checked (VSEARCH v2.16.0 against SILVA v138).
identifiers.namespace: SUB15802606
identifiers.id: XF05