title: IT-scC&T-seq of LMNB1 in a mix of H1, K562, HEK293T and MSCs
design: Library strategy: CUT&Tag; Library selection: CUT&Tag. Indexed tagmentation-based single-cell CUT&Tag (IT-scC&T-seq) was performed to profile LMNB1 binding in four human cell types: H1 embryonic stem cells, HEK293T, K562, and H1-derived mesenchymal stem cells. For each sample, approximately 200,000 0.1% FMA fixed cells were used to extract nuclei, followed by incubation with anti-Lamin B1 antibody (Abcam, ab239399). After secondary antibody incubation, indexed pA-Tn5 transposase was used for tagmentation. Indexed nuclei were sorted into 384-well plates, and PCR amplification was performed in two rounds to introduce additional indexes. Libraries were pooled, purified, and subjected to quality control before sequencing on the Illumina NovaSeq platform.
identifiers.namespace: SUB15377797
identifiers.id: 4CellLB1