title: GSM8664000: Txp3mo_MES_eGBO; Homo sapiens; RNA-Seq
library_construction_protocol: Approximately ten organoids per condition were pooled and dissociated in TrypLE for 15min with gentle agitation every 5min. Single cell solutions were resuspended in 200µL of cell staining buffer (BioLegend, 420201) plus 1µg of TotalSeq-A hashtag antibodies (BioLegend, 399907) and incubated for 30min on ice. After three washes in 1mL of cell staining buffer, cells were resuspended in DMEM at a concentration of 1000cells/µL and pooled together. Recovered tumors were dissociated using the Brain Tumor Dissociation Kit (Miltenyi Biotec, 130-095-942). Myelin was removed using Myelin For library preparations, the Chromium Next GEM Single Cell 3ʹ Reagent Kit v3.1 (10x Genomics) was used following the provided protocol. The library was sequenced with a NovaSeq 6000 System (Illumina) at the recommended 26x98bp.
identifiers.namespace: GEO
identifiers.id: GSM8664000_r1