title: GSM8611926: scRNA-seq part 5; Homo sapiens; RNA-Seq
library_construction_protocol: Firstly, we created the CROPi-DAVs plasmid library, and co-transfected into HEK-293T cells. Lentivirus was collected at 48h post-transfection.  For library infection, CRISPRi_HepG2 cells were infected with CROPi-DAVs lentivirus library. Puromycin screening was performed at 48h post-infection and following by maintaining for another 7 days . After puromycin selection and expansion, cells were captured by the 10X Chromium Controller using Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (10X Genomics, PN-1000268). The sgRNA-enrichment library were constructed by multiple-PCR using using 2x KAPA HiFi HotStart ReadyMix.
identifiers.namespace: GEO
identifiers.id: GSM8611926_r1