title: GSM8805459: TCR from CD8 T cell PDAC; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were excised and placed in cold RPMI-1640 media supplemented with 5% FBS (RP5). Cell suspensions were generated by finely mincing tumor samples into 1mm by 1mm pieces using razor blades and samples were digested for 30 minutes at 37degress as previously described (PMID 32024640). Samples were stained with olgio-hashtag antibodies and viable CD45+CD3+CD8+ single cells were sorted from orthotopic KPC-4662 mice. Single cell prepration was performed using 10X Genomics Chromium Controller and the 5' GEM, Library & Gel Bead Kit v2 (PN-1000263). Barcoded products were purified and double-sided size selected with magnetic beads to isolate cDNA and hashtag DNA products following manufacturer's recommendation. 10x Genomics Chromium Controller and Chromium Single Cell 5' GEM, Library, and Gel bead kit v2 was used following manufacturers protocols.
identifiers.namespace: GEO
identifiers.id: GSM8805459_r1