title: Amplicon sequencing of ARRB1 locus in PLB-985 ARRB1 ARRB2 double KO clone E3
design: Genomic DNA was harvested from five million cells (Invitrogen Purelink Genomic DNA Kit). The purified gDNA was then used as a PCR template for primers that flank the CRISPR cut site. The forward primer sequence was 5-ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCCACAGAGCTTGGGACAG, and the reverse primer sequence was 5-GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCTCACCGCCCTCTTATG. The PCR product was cleaned (Zymo DNA clean and concentrator-5) and then sent to Genewiz for 2 x 250 bp amplicon sequencing (Amplicon-EZ service).
identifiers.namespace: SUB15239417
identifiers.id: SRC4_CloneE3