title: GSM8804768: VCaP ERG shRNA#2; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% v/v formaldehyde for 15 minutes and quenched with 2M Glycine for 5 minutes. Cells were then lysed and sonicated using a Diagenode Biorupter Pico. ChIP-Seq libraries were generated by pooling at least three ChIPs per condition and sonicating to approximately 150bps. 10ng of IP was used as starting material and fragment end repair was done using Klenow DNA polymerase, T4 DNA ploymerase, and T4 DNA Ligase followed by QIAQuick PCR Purification. TruSeq adaptors were ligated via T4 DNA Ligase after which libraries were amplified through 18 cycles and products were size selected on a 2% agarose gel and AMPure XP bead cleanup.
identifiers.namespace: GEO
identifiers.id: GSM8804768_r1