title: 16S rRNA amplicon-seq: Aerobic pond
design: DNA was extracted from the sample using the NucleoSpin Soil DNA Extraction kit (Macherey-Nagel, Germany) following the manufacturer's instructions. Then, PCR amplification targeting V3 and V4 hypervariable region were performed using 341F and 785R using the KAPA HiFi HotStart ReadyMix (KAPABiosystems, United States).
identifiers.namespace: SUB15166854
identifiers.id: Amplicon_aerobic