title: GSM8839394: Isoleucine deprivation, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cells were washed with cold PBS containing cycloheximide and lysed in polysome lysis buffer (20 mM Tris-HCl pH 7.4, 150 mM NaCl, 5 mM MgCl₂, 1% Triton X-100, 5 mM DTT, 100 µg/ml CHX, DNase, RNase inhibitor, protease/phosphatase inhibitors). Lysates were cleared by centrifugation and RNA was extracted via phenol-chloroform and ethanol precipitation. NEBNext Ultra II Directional RNA Library Prep with PolyA selection
identifiers.namespace: GEO
identifiers.id: GSM8839394_r1