title: DNA sequence from fungal mock community (Basidiom.)
design: Genomic DNA samples of individual fungal species were pooled at equal DNA proportion to the final concentration of 5ng/l. PCR amplifications were performed using ITS3 (AGGCATCGATGAAGAACGCAGC) and ITS4 (TCCTCCGCTTATTGATATGC) primers with MyTaq Red DNA Polymerase (Bioline, Taunton, MA, USA). Amplicon library was prepared using a TruSeq DNA PCRfree Kit (Illumina, San Diego, CA, USA). Sequencing was done on an Illumina MiSeq platform using the MiSeq Reagent Kit v2 for 2x250 bp reads.
identifiers.namespace: SUB15250462
identifiers.id: SHA012