title: GSM8612008: UOK109 cells, NONOmut::TFE3 rescue, Rep3; Homo sapiens; ATAC-seq
library_construction_protocol: We used the Illumina Tagment DNA Enzyme and Buffer Kit (20034197, Illumina). We used 50,000 cells for each reaction. Upon transposition, we cleaned up the reaction using a Zymo DNA Clean and Concentrator-5 Kit (D4014, Zymo) according to the manufacturer's protocol. We amplified the eluted DNA using 2x NEBNext Q5 Hotstart HiFi PCR Master Mix (M0543L, NEB) for 11 cycles. We purified the amplified libraries using the Zymo DNA Clean and Concentrator-5 Kit.
identifiers.namespace: GEO
identifiers.id: GSM8612008_r1