title: GSM8701282: IgG rep2; Toxoplasma gondii; OTHER
library_construction_protocol: Cleavage Under Targets and Tagmentation was performed by using commercial Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (TD904, Vazyme Biotech) according to the manufacturer's instructions. The strain, RH iAP2XI-3-mAID-3HA was collected and bounded to Concanavalin A-coated beads. The anti-HA tag antibody (ab9110, Abcam) was used as primary antibody. After incubation of primary and secondary antibodies, the samples were subjected to transposon activation and tagmentation. Then targeted DNA was isolated, amplified, and purified to construct library by using VAHTS DNA Clean Beads (N411, Vazyme Biotech). Finally, the library was quantified and sequenced on the Illumina novaseq 6000 sequencer with the PE150 model.
identifiers.namespace: GEO
identifiers.id: GSM8701282_r1