title: RNA-Seq
design: PCR amplicon library construction refers to the method of adding splices at both ends of the DNA fragment to be tested by PCR reaction. The principle is relatively simple, and only two rounds of PCR and two steps of purification are needed to obtain the library of the target region. The first step is to bind the primer containing the common sequence to the target region; the second step is to connect the sequencing splices through PCR reaction. The constructed library can be used for computer sequencing.
identifiers.namespace: SUB14949359
identifiers.id: shYTHDF2_2_L1