title: GSM9088154: WT rat skin: DHT+  FeCl₃, replicate 2; Rattus norvegicus; RNA-Seq
library_construction_protocol: For bulk RNA-seq: Total RNA was extracted from skin tissue using the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. For scRNA-seq: Cell pellets were obtained from dissociated skin tissue using enzymatic digestion, followed by centrifugation. Cell pellets were directly used for RNA extraction or single-cell library preparation. For bulk RNA-seq, RNA-seq libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB), following polyA enrichment or rRNA depletion. For scRNA-seq, chromium Single Cell 3' Reagent Kits v3.1 (10x Genomics) were used.
identifiers.namespace: GEO
identifiers.id: GSM9088154_r1