title: RNA-seq of bacteriaRuegeria pomeroyi DSS-3
design: For prokaryotic samples, ribosomal RNA was removed from the total RNA and then precipitated with ethanol. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.
identifiers.namespace: SUB15665537
identifiers.id: TMA_rep3