title: GSM8741175: 1-MetLung_1; Mus musculus; RNA-Seq
library_construction_protocol: The concentration and viability of the single cell suspension was measured using acridine orange (AO) and propidium iodide (PI) and the Luna-FX7 Automatic Cell Counter. Approximately (10,000 cells were loaded on Chromium Chip and partitioned in nanolitre scale droplets using the Chromium Controller and Chromium (4)Next GEM Single Cell Reagents (CG000315 Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index)). Within each droplet the cells were lysed, and the RNA was reverse transcribed. All of the resulting cDNA within a droplet shared the same cell barcode. Illumina compatible libraries were generated from the cDNA using Chromium Next GEM Single Cell library reagents in accordance with the manufacturer's instructions (10x Genomics, CG000315 Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index)). Final libraries are QC'd using the Agilent TapeStation and sequenced using the Illumina  NovaSeq 6000. Sequencing read configuration: 28-10-10-90.
identifiers.namespace: GEO
identifiers.id: GSM8741175_r1