title: GSM8741510: BV-2 cells, aged, LPS treated, N2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the RNeasy Plus Mini kit (Qiagen) with an additional step of Dnase treatment. The yield and quality of the isolated RNA were analysed using NanoDrop spectrophotometer. Total RNA samples were subjected to quality control with Agilent Tapestation according to the manufacturer's instructions. To construct libraries suitable for Illumina sequencing, the Illumina stranded mRNA prep ligation sample preparation protocol was used with starting concentration of 200 ng total RNA. The protocol includes mRNA isolation, cDNA synthesis, ligation of adapters and amplification of indexed libraries. The yield and quality of the amplified libraries were analysed using Qubit by ThermoFisher Scientific and quality was further checked by using Agilent Tapestation. The indexed cDNA libraries were normalized and combined, and the pools were sequenced.
identifiers.namespace: GEO
identifiers.id: GSM8741510_r1