title: PrimerID of YEZV: 0  NHC - rep 2
design: Three sequence-specific RT primers were designed with Nextera adapters and a UMI at the 5' end. PCR was carried out using the adapter primer and a sequence-specific reverse primer containing the other Nextera adapter sequence at its 5' end. A second round of PCR (6 cycles) was carried out, per Illumina DNA Flex instructions, using Illumina CD DNA plated index primers.
identifiers.namespace: SUB15638507
identifiers.id: YEZV-0-NHC-2