title: GSM8740392: HepG2-IGF1R-KO_normal-media_human-insulin-stimulation_72hr_BiolRep3; Homo sapiens; RNA-Seq
library_construction_protocol: RNA for bulk RNA-seq was isolated using RNeasy Mini Kit with DNase treatment according to manufacturer's instructions (Qiagen, Hilden, Germany) The sequencing library was prepared by purification of poly-A containing mRNAs, mRNA fragmentation, strand-specific random primed cDNA synthesis, and adapter ligation and adapter specific PCR amplification using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7765, New England BioLabs, Ipswich, Massachusetts, USA)
identifiers.namespace: GEO
identifiers.id: GSM8740392_r1