title: GSM8879202: TDG_PCP2Cre_positive_Purkinje_gDNA_Grid2_6loci_PS-seq; Mus musculus; OTHER
library_construction_protocol: For pyridine borane-sequencing, genomic DNA was extracted from day 7 iNs or sorted Purkinje nuclei using DNeasy® Blood & Tissue (QIAGEN; cat. No. 69504) following the manufacturer's instructions. For pyridine borane-sequencing, 200 ng genomic DNA was used in a 50 μl reaction containing 600 mM sodium acetate solution (3M, pH = 4.5) (Quality Biological, cat. No. 351-309-101) and 1 M pyridine borane (Alfa Aesar, cat. No. L13178-09), and incubated for 16 hours at 37°C in an Eppendorf ThermoMixer shaking at 850 rpm. The product was purified by the QIAquick Nucleotide Removal Kit (QIAGEN, cat. No. 28306). The primers targeting the desired neuronal enhancer regions containing CpG sites were designed and the PCR amplicons were generated using the PyroMark PCR kit (QIAGEN, cat. No. 978703).
identifiers.namespace: GEO
identifiers.id: GSM8879202_r1