title: GSM9181569: HF_HM51rep1,HF_HM51rep2; Mus musculus; RNA-Seq
library_construction_protocol: Collect the livers of mice from each treatment group and extract RNA using Trizol. Total RNA was extracted and assessed for quality and quantity. mRNA was enriched using poly(A) selection with Oligo(dT) beads. The mRNA was then fragmented and reverse transcribed into cDNA using random primers. After second-strand synthesis, end repair and adapter ligation were performed. The resulting libraries were purified and amplified by PCR. Sequencing was carried out on the Illumina NovaSeq  platform.
identifiers.namespace: GEO
identifiers.id: GSM9181569_r1