title: GSM9244200: TR065_3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted using the miRNeasy Mini Kit (QIAGEN) following the manufacturer's protocol, and RNA quality was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). Employing the Switching Mechanism at 5' End of RNA Template (SMART) technology to add adapter sequences to both ends of the first-strand cDNA, full-length cDNA was synthesized using the SMART-Seq mRNA Kit (Takara Bio USA, Inc.), followed by PCR amplification using primers recognizing the adapter sequences. Subsequently, the double-stranded cDNA was fragmented and tagged at both ends through a tagmentation reaction using the Nextera XT DNA Library Prep Kit (Illumina Inc.), which applies transposon-mediated fragmentation and adapter insertion.Amplified libraries were generated using index primers (IDT for Illumina - DNA/RNA UD Indexes, Integrated DNA Technologies, Inc.).
identifiers.namespace: GEO
identifiers.id: GSM9244200_r1