title: GSM8585416: H1299 cells, shWDR4 exosomes, rep2; Homo sapiens; miRNA-Seq
library_construction_protocol: miRNA was harvested with High Pure miRNA Isolation Kit (Roche). Sequencing libraries were generated using QIAseq® miRNA Library Kit (QIAGEN, Germany) following the manufacturer's recommendations. Briefly, 3' and 5' adaptors were directly and specifically ligated to 3' and 5' end of small RNA, respectively. Then first-strand cDNA was synthesized using QIAseq miRNA NGS RT Enzyme and RT primer. After PCR amplification, the library was size-selected with 170 - 200 bp by QIAseq beads. The quality and quantity of purified libraries were assessed on the Qsep400 system (Bioptic Inc., Taiwan) and Qubit 2.0 Fluorometer (Thermo Scientific, Waltham, MA, USA).
identifiers.namespace: GEO
identifiers.id: GSM8585416_r1