title: GSM8804940: RNA-seq_cdPRDM9_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: 1 x 106 cells were harvested in 1 mL TRIzol reagent (Thermo Fisher Scientific) and homogenized by pipetting. After incubation at room temperature for 5 min, 100 uL BCP was added. Samples were vortexed and incubated briefly at room temperature and centrifuged at 12,000 × g, 15 min, 4°C. The aqueous phase was transferred to a new tube and total RNA was isolated with the RNeasy Micro Plus Kit (Qiagen) following the manufacturer's instructions. Purified RNA was used for sequencing library prep with the KAPA RNA HyperPrep Kit with RiboErase (Roche KR1351).
identifiers.namespace: GEO
identifiers.id: GSM8804940_r1