title: GSM8612019: UOK109 cells, NONOdeltaCCD::TFE3 rescue, FLAG, Rep2; Homo sapiens; OTHER
library_construction_protocol: We lightly fixed the cells with 0.4% w/v PFA for 1 min and quenched using 0.125 M glycine for 5 min. Cells were pelleted and washed once with PBS. Cell pellets were snap-frozen and stored in -80 ºC freezer until being processed. CUT&RUN were performed with Epicypher's CUTANA CUT&RUN kit v3 and library preparation kit v1.1 following the manufacturer's instructions using a FLAG antibody (CST 14793, Cell Signaling) and H3K27Ac antibody (39034, Active Motif). No E. coli spike-in was used. Library quality and concentrations were assessed using the D1000 TapeStation system (Agilent). Libraries were sequenced for 25 cycles in 75-bp paired-end mode on Illumina Novaseq 6000 and data were analyzed as described.
identifiers.namespace: GEO
identifiers.id: GSM8612019_r1