title: GSM8770934: Ctcf_2ildtag24h_rep1; Mus musculus; RNA-Seq
library_construction_protocol: Crosslinked cells were processed by Covaris truChIP Chromatin Shearing kit and chromatin were sonicated to 300-500bp by Covaris M220. After cell harvesting genomic DNA was isolated as described before (Sanjana et al., 2014 and Shalem et al., 2014). Total RNA from cells was extracted using the TRIzol Plus RNA purification kit (Thermo Fisher Scientific, Catlog: 12183555). ChIP DNA and Input DNA were constucted into illumina Tru-seq libraries using VAHTS Universal DNA Prep Kit for Illumina V4 (ND610-01) CRISPR libraries were prepared on sorted populations according to previously described protocol mainly consisting of two-step PCRs (Sanjana et al., 2014 and Shalem et al., 2014) RNA-Seq libraries were prepared from 4 µg total RNA using the Illumina TruSeq Stranded mRNA Library Prep Kit Set A (RS-122-2101; Illumina) or Set B (RS-122-2102; Illumina). RNA-seq libraries were sequenced on illumina Next-seq 2000  (100-bp paired ends).
identifiers.namespace: GEO
identifiers.id: GSM8770934_r1