title: GSM9244908: Pooled library; Mus musculus; OTHER
library_construction_protocol: RNA extracted with Zymo quick-RNA MicroPrep kit according to the manufactuer's instructions Library preparation was performed starting with 30 ng of total RNA of each sample using the HS BRB-seq Library Preparation Kit (Cat. #10891; Alithea Genomics, Epalinges, Switzerland). Subsequently, 14 ng of cDNA was used for tagmentation. An additional PCR (5 cycles) was carried out following the BRB-seq protocol, employing the Equinox Library Amplification Kit (Cat. #7K0014-096; Watchmaker Genomics, Boulder, CO, USA). Final libraries were sequenced on an AVITI platform (Element Biosciences, San Diego, CA, USA) with the AVITI 2×75 Sequencing Kit Cloudbreak FS High Output (Cat. #860-00015) in paired-end mode (29 bp read 1 / 121 bp read 2), applying a PMG shift of 2 during run setup.
identifiers.namespace: GEO
identifiers.id: GSM9244908_r1