title: GSM9087638: Calm1(+/+) mouse_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the left ventricular tissues of Calm1+/+, Calm1N98S/+ and Calm1N98S/N98S (N = 3 per genotype per group) with Trizol (Thermo Scientific) Agilent RNA 6000 Nano Kit and Agilent 2100 bioanalyzer (Agilent Technologies) were utilized to assess the quality and integrity of the total RNA samples. Only RNA samples with an RNA integrity number of at least 8 were used for subsequent library construction and sequencing. Mouse left ventricular RNA (1 g total RNA for each sample) sequencing libraries were constructed using a SureSelect Strand-Specific RNA library prep kit (Agilent Technologies)
identifiers.namespace: GEO
identifiers.id: GSM9087638_r1