title: GSM8878923: HdFbs at 12th passage, 5mg/mL collagen, 30mM D-ribose, 10days, 1replicate; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from cells was isolated using Quick-RNA MiniPrep Kit (Zymo Research, USA) according to the manufacturer's instructions. The 3D gels with cells were pre-treated with Collagenase Type II (Gibco, USA) at a concentration of 150 U/ml and kept in a CO2 incubator until the collagen had visually dissolved (approximately 40 minutes). To obtain mRNA, total RNA were enriched by Poly(A) using VAHTS mRNA Capture Beads (Vazyme Biotech, Сhina). Double-stranded cDNA libraries were prepared using VAHTS® Universal V8 RNA-seq Library Prep Kit for Illumina (Vazyme Biotech) according to the manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8878923_r1