title: RNA-Seq of Hordeum vulgare
design: The apex and leaf tissues were harvested from the main shoots. Samples were collected three hours after lightening and three hours after darkening of climate chamber at 11 days, 19 days and 33 days after germination (DAG). For sampling the apex, the leaves surrounding the MSA were removed manually, and the apex was cut with a Paragon Nr.10 microsurgical disposable stab knife under a stereo microscope. From each tissue, a minimum of three pooled biological replicates were taken per time-point for each DH line. Each of the MSA samples collected during the vegetative phase W1.0 consisted of 30 pooled apices. During reproductive stages W1.5-2.0 and W3.5, pool of 15 and 11 shoot apices were collected, respectively. The leaf samples were harvested from a subset of 10 plants, from which apex tissue was collected. The harvested leaf tissue was restricted to the distal part of the leaf, at 2 to 4 cm before the leaf tip. On average, RNA-seq sample collection was completed within 3 hours each time. For each day the two apex pools from light and dark time-points of each genotype were mixed to mitigate the possibility of difference in gene expression in presence and absence of light.
identifiers.namespace: SUB14823968
identifiers.id: Sample29