title: ITS-Metagenome
design: Soil genomic DNA (gDNA) was extracted from 0.25 g rhizosphere soil samples using the PowerSoil PRO DNA Isolation Kit (Qiagen, Hilden, Germany) following the manufacturer's instructions. The quantity and quality of extracted gDNA were verified using NanoDrop (Thermo Scientific, Wilmington, DE) and electrophoresis (1% agarose gel, including a 1 kb plus ladder). PCR amplification was performed for each soil DNA extract in triplicate and combined into a single composite sample. Amplicons targeting the V5V7 region of the 16S rRNA gene (799F1193R) (Bodenhausen et al., 2013) and the ITS2 region (FITS7-ITS4R, (1) were obtained by PCR to characterize bacterial and fungal communities, respectively, according to  Qiu et al. (2020). Amplicon sequencing was performed at the Ramaciotti Centre for Genomics at the University of New South Wales (Sydney, Australia), using the Illumina NextSeQ (PE 300 bp) platform
identifiers.namespace: SUB15438775
identifiers.id: AY48-F47-XIO14409A47-AAG5YFWM5_S41_L001