title: RNA-sequencing of normal breast tissue samples before and after 12-week anti-progestin (ulipristal acetate) treatment
design: RNA-sequencing of normal breast tissue samples before and after 12-week anti-progestin (ulipristal acetate) treatment
library_construction_protocol: Normal breast tissue cores obtained via Vacuum-Assisted Biopsy (VAB) with a 10G biopsy needle were snap frozen for RNA extraction. A baseline VAB was performed in the luteal phase of the menstrual cycle and a second VAB was performed after a 12-week anti-progestin (ulipristal acetate, daily 5mg oral tablet) treatment.  RNA was extracted using the RNeasy Plus Micro Kit (#74034, Qiagen) as described in the manufacturerâ€™s handbook. Indexed PolyA libraries were prepared using 100ng of total RNA and 15 cycles of amplification in the Agilent Sure Select Strand Specific RNA Library Prep Kit for Illumina Sequencing (Agilent Cat No: G9691B). Libraries were quantified by qPCR using a Kapa Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems Inc. Cat No: KK4873).
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-13720:BAP22T1_p