title: CK-3 (CRX1419391; CRA022123)
library_construction_protocol: The total RNA was extracted from six samples (two groups: CK and 4 mM, three replicates) with pBIOZOL Plant Total RNA kit (BioFlux, Hangzhou, China) following the manufacturer's instructions. Then, the mRNA with polyA tails was selectively enriched using Oligo (dT) magnetic beads, and fragmented into short segments using a fragmentation buffer. These fragments served as templates for the synthesis of first-strand cDNA, followed by the second-strand cDNA. Subsequently, the purified double-stranded cDNA underwent end repair, A-tailing, sequencing adaptor ligation, fragment selection (250-350 bp) and PCR enrichment to ultimately obtain the cDNA library. Finally, the Illumina HiSeq2000 platform (Illumina, USA) was applied for sequencing, generating 150 bp paired-end reads.
identifiers.namespace: NGDC
identifiers.id: CRX1419391
identifiers.namespace: Nanjing Agricultural University
identifiers.id: DRX725733