title: GSM8839530: P28 retina, H3K18La (CUT&Tag), replicate 1; Mus musculus; OTHER
library_construction_protocol: CUT&Tag protocol was performed using CUT&Tag-IT® Assay Kit – Tissue (Active Motif) with slight modifications. In brief, retinas were dissected at P4, P10 and P28 and cells were dissociated in a 5 ml polypropylene round-bottom tube with a papain dissociation protocol adapted from previous study (Fadl, et al., Mol Vis. 2020) . After dissociation the cells were washed using 1X wash buffer provided in the kit at 200g for 5 minutes. CUT&Tag was performed using 500,000 cells per experiment following kit protocol. Antibodies against H3K18La (Rabbit, cat.no. PTM1406), and H3K27Ac (1:00, monoclonal Antibody (2D7B3) Invitrogen) were used at a concentration of 1:100 in 100 μl and pA-MNase conjugated to protein A (generous gift of Dr. Steven Henikoff, Howard Hughes Medical Institute, Washington, USA) used at a concentration of 700 ng/ml. CUT&Tag-IT® Assay Kit – Tissue (Active Motif)
identifiers.namespace: GEO
identifiers.id: GSM8839530_r1