title: GSM8644287: BLM_2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the TRK1002U Kit (LC Sciences) according to the manufacturer's protocol. The total RNA quantity and purity were analyzed by NanoDrop ND-1000 (NanoDrop). The polyA-tailed RNA was captured by the specific capture using Dynabeads Oligo (dT) (Thermo Fisher), and fragmented by a Magnesium RNA Fragmentation Module(NEBNext®). cDNA was synthesized by Reverse Transcriptase (Invitrogen), followed by two-stranded synthesis of double-stranded DNA with adding dUTP（Thermo Fisher). The end repair and adenlation of the double-stranded DNA were performed. The polymerase chain reaction (PCR) was carried out and the library was constructed. Finally, the paired-end sequencing was performed using illumina Novaseq™ 6000 (LC Bio Technology CO., Ltd).
identifiers.namespace: GEO
identifiers.id: GSM8644287_r1