title: 16S of female vaginal samples of patients recruited from women's health clinic
design: DNA extraction was performed using the DNeasy PowerSoil Pro Kit (Qiagen), as per the manufacturers instructions. After DNA extraction, the DNA from the clinician-collected vaginal swabs underwent 16s rRNA sequencing. The 16s rRNA gene was amplified using the V4 hypervariable region, with the primers 515F (GTGCCAGCMGCCGCGGTAA) and 806-R (GGACTACHVGGGTWTCTAAT) [82]. The primers included Illumina adapters and reverse primers included a 12-base pairs (BP) barcode which is unique to each sample. PCR products were then cleaned with QIAseq beads (Qiagen, Hilden, Germany), and quantified using the QUANT-iT PicoGreen dsDNA Assay Kit (Invitrogen, Waltham, MA, USA). Pooled purified DNA products were sequenced on a 2 x 150 bp Illumina Nextseq 1000 platform (Illumina, San Diego, CA, USA) at the PANDA Core for Genomics and Microbiome Research, University of Arizona, USA.
identifiers.namespace: SUB14905891
identifiers.id: 506