title: GSM8740057: NMFs from healthy C57BL6 mice repetition 2; Mus musculus; RNA-Seq
library_construction_protocol: NMFs were collected from healthy 8-12 week old BALB/c or C57BL/6 females. Fat pad tissue was minced and dissociated using the gentleMACS dissociator with an enzymatic digestion solution consisting of 1 mg/ml collagenase II, 1 mg/ml collagenase IV, and 70 U ml−1 DNase in DMEM. The samples were filtered through a 70-μm cell strainer into ice-cold MACS buffer (PBS with 0.5% BSA and 0.4% EDTA pH=8) and cells were pelleted by centrifugation at 350g for 5 min at 4 °C followed by Red Blood lysis treatment and seeding onto a cell-culture treated plate pre-coated with rat-tail collagen type I. 3' end mRNA libraries were prepared for sequencing using a method developed at Dr. Ido Amit's lab, Weizmann Institute of Science
identifiers.namespace: GEO
identifiers.id: GSM8740057_r1