title: GSM9511080: B2M tdTomato K-562 cells treated with a poly-functional editor targeting AAVS1 and epi-silencing B2M, replicate 3.; Homo sapiens; OTHER
library_construction_protocol: gDNA extraction by Maxwell RSC Cultured cells DNA Kit (Promega), following provider indications. Targeted genomic regions were amplified by PCR using locus-specific primers. A second round of PCR was performed to add full-length Illumina adapters and sample-specific barcodes. Libraries were beads-purified, quantified, pooled at equimolar concentrations, and sequenced using an Illumina MiSeq platform in a paired-end mode generating 2x250 bp reads.
identifiers.namespace: GEO
identifiers.id: GSM9511080_r1