title: GSM8644471: WT3_201_1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted using Qiazol (QIAGEN #79306). 1 ug of total RNA was used for the construction of sequencing libraries. Libraries were prepared using Novogene's property library prep protocols (Novogene NGS RNA Library Prep Set PT042 for 250-300 bp insert cDNA library). Indices were incorporated to multiplex multiple samples. mRNA was purified using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by a second strand cDNA synthesis. Library preparation was finalized following end repair, A-tailing, adapter ligation, and size selection. After amplification and purification, insert size of the library was validated on an Agilent 2100 and quantified using RT-PCR. Libraries were sequenced using Illumina NovaSeq 6000 S4 flowcell with PE150.
identifiers.namespace: GEO
identifiers.id: GSM8644471_r1