title: RNA-seq of Gasterosteus aculeatus: female larvae
design: We staged samples and preserved in RNAlater Stabilization Solution (Invitrogen AM7021) following manufacturer guidelines and stored at -80 C until analysis. We extracted RNA using TRIzol Reagent (Invitrogen 15596026) and the Direct-zol RNA Microprep Kit (Zymo R2062). We created sequencing libraries using the Illumina TruSeq Stranded mRNA Library Prep kit (20020594). We pooled the libraries from all samples and the pooled libraries were sequenced on two lanes using a NovaSeq X Plus by Azenta Life Sciences (South Plainfield, NJ, USA).
identifiers.namespace: SUB15241821
identifiers.id: d8-1