title: GSM8839602: Non-MPR-1; Homo sapiens; RNA-Seq
library_construction_protocol: The Isolation Kit furnished by Shanghai Biotechnology Corporation was exploited. Tissue samples were homogenized and transformed into a liquid state with a tissue homogenizer, succeeded by thorough lysis on ice. The resultant tissue lysates were filtrated through a 40μm cell sieve to eliminate impurities, and the supernatant was meticulously aspirated by means of centrifugation while preserving the precipitate. Nuclear suspensions were obtained in accordance with the standard protocols of the kit. Ultimately, 5μL of each nuclear suspension was aliquoted and stained with trypan blue for the quantification of cell nuclei and microscopic examination. The Gel Beads-in-emulsion was transferred to a PCR instrument for reverse transcription in Shanghai Biotechnology Corporation, and the cDNA strand with Barcode and UMI information was obtained according to the standard procedure of the system, followed by purification, amplification and detection of concentration. After passing the quality inspection, cluster generation and hybridization of the first sequencing primers were completed, and the flow cell with cluster was carried on the computer.
identifiers.namespace: GEO
identifiers.id: GSM8839602_r1