title: GSM8584483: PBMC CON rep4; Homo sapiens; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using TRIzol Reagent (Invitrogen). RNAs were treated with T4PNK, then AlkB before cDNA library construction.The RNA segment was separated by PAGE, then a 15- to 45-nucleotide stripe was selected and recycled. The adapters were obtained from the QIAseq® miRNA Library Kit (QIAGEN: 331505) and ligated sequentially. The amplified flow cell was sequenced on the Illumina system by epibiotek (Guangzhou Epibiotek Co., Ltd., Guangzhou, China).
identifiers.namespace: GEO
identifiers.id: GSM8584483_r1