title: GSM8701577: EV-1; Homo sapiens; ATAC-seq
library_construction_protocol: MCF10A cells (empty plasmid vector, H2B variants expressing cells, or transformed cells with EMT-inducing cytokines) were grown to 70-90% confluency, cryopreserved in DMEM/F12 media containing 50% horse serum, and sent to ActiveMotif (Carlsbad, CA) for ATAC sequencing. 10^5 cells were resuspended in lysis buffer, then centrifiuged to collect nuclei from which genomic DNA was obatined. Libraries used for ATAC-sequencing were prepared using the Illumina Nextera Library Prep Kit.DNA fragments were then purified with a Qiagen MinElute PCR purification kit. After 10 PCR cycles amplification of the tagged DNA fragments, the amplified products were purified with Beckman Coulter Agencourt AMPure SPRI beads. For quantification, the KAPA Library Quantification Kit was used. ATAC-seq libraries were prepared using the Illumina Nextera Library Prep Kit, and the resulting DNA fragments purified with a Qiagen MinElute PCR purification kit. Tagged DNA fragments were amplified through 10 PCR cycles, and amplified products purified
identifiers.namespace: GEO
identifiers.id: GSM8701577_r1