title: GSM8902425: JT06; Homo sapiens; RNA-Seq
library_construction_protocol: The tissue samples were processed as described below. Briefly, samples were first washed with phosphate-buffered saline (PBS), minced into small pieces on ice After washing with PBS containing 0.04% BSA, the cell pellets were re-suspended in PBS containing 0.04% BSA and re-filtered through a 35 μm cell strainer. Dissociated single cells were then stained with AO/PI for viability assessment using Countstar Fluorescence Cell Analyzer. The single-cell suspension was further enriched with a MACS dead cell removal kit (Miltenyi Biotec). BD Rhapsody system  were used to capture the single cells and construct the scRNA-Seq libraries
identifiers.namespace: GEO
identifiers.id: GSM8902425_r1