title: GSM9449473: MCF10A shHPA rep1; Homo sapiens; Hi-C
library_construction_protocol: Total RNA was isolated using the Qiagen RNeasy Plus kit following manufacturer's instructions. HiC Libraries were prepared in duplicates for each cell type using the Arima-HiC+ kit (Arima Genomics, USA) according to manufacturer instructions. For each cell line, 1 million cells were used per HiC reaction. Cells were fixed with 1.2% formaldehyde for 12 min. All subsequent steps were carried out according to the Arima protocol. Resulting libraries were amplified with 5 PCR cycles and sequenced on an Illumina NovaSeq 6000 instrument in paired-end run with 2×101 bp. Total RNA was isolated using the Qiagen RNeasy Plus kit following manufacturer's instructions. HiC Libraries were prepared in duplicates for each cell type using the Arima-HiC+ kit (Arima Genomics, USA) according to manufacturer instructions. For each cell line, 1 million cells were used per HiC reaction. Cells were fixed with 1.2% formaldehyde for 12 min. All subsequent steps were carried out according to the Arima protocol. Resulting libraries were amplified with 5 PCR cycles and sequenced on an Illumina NovaSeq 6000 instrument in paired-end run with 2×101 bp.
identifiers.namespace: GEO
identifiers.id: GSM9449473_r1