title: GSM8585341: Bioprint, trophoblast, scRNA-seq; Homo sapiens; RNA-Seq
library_construction_protocol: Seven wells of each of bioprinted and Matrigel-embedded organoids were harvested and pooled, producing approximately 700 organoids in total. Organoids were washed twice with PBS and centrifuged for 5 mins at 300 rcf. Organoids were dissociated to single cells by adding 1 mL Accutase to each pooled sample, followed by incubation on a shaker for 30 mins at 800 rpm. The cell suspension was centrifuged for 5 mins at 300 rcf and washed twice with sterile 0.04% BSA in PBS, centrifuging between each step. Cells were then resuspended in 100 µL 0.04% BSA and passed through a 40 µm filter. Cell viability and count was assessed using trypan blue, ensuring viability was > 80 %. Library was perfromed according to the manufacturer's instruction (single-cell 3' v2 protocol, 10x Genomics)
identifiers.namespace: GEO
identifiers.id: GSM8585341_r1