title: tC19Z_LT_5_10
design: RNA generated from extension of an RNA primer along an RNA template under varying conditions was desalted using G15 Sephadex. An adenylylated oligo acting as an adapter was used to introduce a constant region for subsequent reverse transcription to generate cDNA. The cDNAs were then ligated to an additional sequence containing a 7-nt random region and a carbon spacer. A nested PCR reaction was done to enrich for the desired sequences, then an additional PCR reaction was done to introduce the Illumina adapters. The library was then sequenced.
identifiers.namespace: SUB14798557
identifiers.id: 5_10