title: GSM8876046: C26_REV-ERBa_Rep_10; Mus musculus; RNA-Seq
library_construction_protocol: For RNA isolation from tissue, ~200 mg heart tissue was lysed in TRIzol using the TissueLyser II (Qiagen, #85300) followed by RNA purification using EconoSpin columns (Epoch). RNA concentrations were determined using the Nanodrop 2000 (Thermo Fisher). Total RNA (50-100ng) was prepared for sequencing using VAHTS Stranded mRNA-seq Library Kit for Illumina (Vazyme NR602) following manufacturer's recommendations, except that no selection for poly-adenylated RNA was used. Prepared PCR products were purified and size-selected on AMPure XP beads (Beckman A63881), and library quality was assessed on the Agilent Bioanalyzer 2100 system. RNA libraries were sequenced as 50 bp paired-end runs on a HiSeq 4000 platform (Illumina, ‎San Diego, USA).
identifiers.namespace: GEO
identifiers.id: GSM8876046_r1