title: GSM8902557: p21257-s042_RHj18_D7; Macaca mulatta; RNA-Seq
library_construction_protocol: Blood samples were collected in PAXgene Blood RNA tubes (BD Biosciences), and RNA was extracted using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit, designed for use with PAXgene Blood RNA Tubes (ThermoFisher Scientific). The quality of the RNA was evaluated with a TapeStation 4200 (Agilent), after which one microgram of total RNA underwent globin transcript depletion using the GLOBINclear Kit, human (ThermoFisher Scientific). For cDNA synthesis, ten nanograms of the globin-depleted RNA were used as input with the Clontech SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio), following the manufacturer's protocol. The amplified cDNA was fragmented and dual-indexed bar codes were added using the Nextera XT DNA Library Preparation kit (Illumina). Libraries were validated through capillary electrophoresis on a TapeStation 4200 (Agilent), pooled in equal concentrations, and sequenced with PE100 reads on an Illumina NovaSeq 6000, with an approximately 40 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM8902557_r1