title: GSM8877134: YM1_HiC; Mus musculus; Hi-C
library_construction_protocol: MuSCs were lysed, digested with restriction enzymes, labeled with biotin, and subjected to proximity ligation. Proximally ligated DNA was purified and fragmented. The fragments were enriched by biotin enrichment beads. Hi-C library was prepared on-bead using the Accel-NGS 2S Plus kit (Swift Biosciences). After adaptor ligation, ligated products were PCR-amplified using KAPA Library Amplification Kit (Roche) and purified. Libraries were combined, and the pooled library was quantified by qPCR using a NEBNext Library Quant Kit (NEB). The final pooled library was paired-end sequenced with read lengths of 150 bp on a NovaSeq 6000 platform (Illumina) using an S2 300-cycle kit.
identifiers.namespace: GEO
identifiers.id: GSM8877134_r1