title: GSM8584529: Upp1-800-GFP(-) cells, Day 2, biol rep 2, RNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: Upp1-800-GFP(+) and (-) cells collected after sorting were subjected to RNA extraction using the NucleoSpin RNA kit (740955, Macherey-Nagel). The DNA was removed by a DNAseI on-column step, as instructed by the kit's manual. RNA-seq libraries were prepared using the NEBNext® Ultra II™ Directional RNA Library prep Kit for Illumina® (E7760, NEB). Sequencing was performed  in the Illumina NovaSeq 6000 sequencer to obtain single-end, reverse-stranded, 100bp-long reads.
identifiers.namespace: GEO
identifiers.id: GSM8584529_r1