title: GSM8772538: Subject 15; Homo sapiens; RNA-Seq
library_construction_protocol: Enrolled subjects underwent flexible fiberoptic bronchoscopy and bronchoalveolar lavage with serial installation and aspiration of normal saline. Lavage fluid was centrifuged to pellet cells. Cell pellet was resuspended  at 1x10^6 cells per ml in PBS containing 0.04% bovine serum albumin. Single cells were captured using The Chromium Box (10x Genomics, Pleasanton, CA). The Chromium Single Cell 3' GEM, library, and gel bead kit PN-1000075 were used along with Bead Chip B PN-1000092 for cell capture and library construction. Libraries were sequenced with the Illumina NovaSEQ 6000 sequencer (Illumina, Inc., San Diego, CA) using an S4 flow cell.
identifiers.namespace: GEO
identifiers.id: GSM8772538