title: Lrat_hM3Dq1
design: HSCs from LratCre;hM3D(Gq)+/- mice were identified as retinol (405nm autofluorescence)+ CD45- and isolated via FACS from liver cells. FACS-sorted HSCs from the liver tissues of C57BL/6 mice were cultured in the Dulbecco's Modified Eagle's Medium (DMEM; Gibco, #C11995500BT) containing 10% fetal bovine serum (FBS; NewZerum, #FBS-UE500), 100 U/ml penicillin, and 100ug/ml streptomycin for more than 7 days. Mouse HSCs were then changed to fresh medium with a final concentration of 10um clozapine n-oxide(CNO, homemade) and cultured for 1 days.
identifiers.namespace: SUB15010359
identifiers.id: Lrat_hM3Dq1