title: GSM8877142: Control LPS injected retina; Mus musculus; RNA-Seq
library_construction_protocol: Single cell suspensions were generated from pooled isolated retinas for each group (n=6) with a Papain Dissociation kit using the protocol provided. The final cell pellet was resuspended in PBS (pH7.4) with 0.1% BSA and cell viability assessed using Trypan blue staining. Only samples with >85% viability were used. Single-cell RNA sequencing (scRNA-seq) was performed using the 10X Genomics platform and reagents (3'v3.1) according to the manufacturer's instructions
identifiers.namespace: GEO
identifiers.id: GSM8877142_r1