title: Whole genome sequencing of mouse cerebellum using nanopore long read sequencing (CBM3_rep2)
design: Cerebellum tissues were obtained from two 8-week-old female C57BL/6J mice, from which high molecular weight genomic DNA was extracted using the QIAGEN MagAttract HMW DNA Kit (QIAGEN, 67563). Cerebellar gDNA was sheared using a Covaris g-TUBE (Covaris, 520079) to a target length of 8 kb. Whole genome sequencing (WGS) libraries were prepared in duplicate for each biological replicate, using the Ligation Sequencing Kit V14 standard protocol (Oxford Nanopore Technologies, SQK-LSK114) with reagents from the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs, E7180S). 150 ng of each sample was loaded onto PromethION (Oxford Nanopore Technologies, PRO-SEQ048) flow cells (Oxford Nanopore Technologies, FLO-PRO114HD). All sequencing runs were manually stopped after 72 hours.
identifiers.namespace: SUB14744083
identifiers.id: CBM3_rep3