title: 16S ribosomal RNA gene sequencing of Homo sapiens: Stool
design: A stool sample was collected in a collection tube provided by the external company CeGaT (Tbingen, Germany) to perform the 16S ribosomal RNA gene sequencing. Twenty ng of DNA were used for library preparation by amplifying the V3-V4 hypervariable regions of the bacterial 16S rRNA gene using the Zymo Quick 16S NGS library preparation kit (Zymo Research). The final libraries were pooled and sequenced with MiSeq Sequencing System (Illumina) for 2x250 base paired-end reads (90.71% bases  Q30). Demultiplexing of the sequencing reads was performed with Illumina bcl2fastq (2.20). Reads were filtered for reads containing Ns and low-quality bases and adapters trimming was performed with Skewer (version 0.2.2). Merging of the reads was performed using R package DADA2. The quality of the FASTQ files was analysed with FastQC (version 0.11.5-cegat).
identifiers.namespace: SUB14770937
identifiers.id: Sample 42