title: Sequel II sequencing
library_construction_protocol: Full-length bacterial 16S rRNA amplicon libraries were prepared by the Integrated Microbiome Resource (IMR) using the 27F(Paliy) (5′-AGRGTTYGATYMTGGCTCAG-3′) and 1492R (5′-RGYTACCTTGTTACGACTT-3′) primer pair targeting the V1–V9 regions, as described in the IMR protocoltps://imr.bio/protocols.html). Libraries were sequenced using PacBio HiFi Circular Consensus Sequencing (CCS) on the Sequel  II platform.
identifiers.namespace: The University of Winnipeg
identifiers.id: ena-EXPERIMENT-TAB-12-11-2025-04:09:15:125-96501