title: GSM8739976: MDA-MB-231, Control shRNA, replicate 1 [ATAC-seq]; Homo sapiens; ATAC-seq
library_construction_protocol: 50,000 cells were washed with PBS and lysed with lysis buffer (10 mM Tris–HCl pH 7.4, 3 mM MgCl2, 10 mM NaCl, 0.1% NP-40, 0.01% Triton X-100 and 0.1% Tween-20) for 10min on ice. Isolated nucleus was incubated at 55°C for 1 h with 2x TD buffer [20 mM Tris–HCl pH 7.6, 10 mM MgCl2 and 20% dimethylformamide] and 2.5 μl of transposase. The transposed DNA was purified using the DNA Clean & Concentrator-5 Kit (Zymo Research, D4003) and then amplified using the NEBNext High-Fidelity 2x PCR Master Mix (NEB, M0541S) for 7 cycles.
identifiers.namespace: GEO
identifiers.id: GSM8739976_r1