title: GSM9244996: mouse NK cells, AP1i stimulated, rep3; Mus musculus; ATAC-seq
library_construction_protocol: ATAC-seq was performed as described previously. Briefly, sorted, stimulated cells were washed in cold PBS and lysed. The transposition reaction was incubated at 37 °C for 30 min. DNA was cleaned with the MinElute PCR Purification kit (QIAGEN, cat. 28004). DNA materials were amplified and the final product was cleaned by AMPure XP beads (Beckman Coulter catalog no. A63882) at a 1× ratio, and size selection was performed at a 0.5× ratio. Libraries were sequenced on a NovaSeq6000 in a 100 bp/100 bp paired-end run, using the NovaSeq6000 S2 or S4 Reagent kit (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM9244996_r1