title: GSM8976947: mRNA-159 - base; Homo sapiens; RNA-Seq
library_construction_protocol: miRNA: The first round of sequencing was performed on NextSeq 2000 sequencer (Illumina, San Diego, CA) to generate a few million reads to quantify the relative concentration of each library. The volume of each library was then adjusted to generate about 5M read clusters from each sample under the same PE 2x61 bp setting for the final data analysis. mRNA: Directional globin mRNA/rRNA depletion RNA-seq was performed using NEBNext Globin & rRNA Depletion Kit (NEB) with 50 to 100 ng whole blood RNA was globin mRNA/rRNA depleted according to the protocol. miRNA: NEBNext Small RNA Sample Library Preparation kit (NEB, Ipswich, MA) was used with modified approach for precise miRNA library size selection; this makes it possible for the kit to process low input and low-quality RNA with better library recovery and miRNA reads alignment.  mRNA: NEBNext Ultra II Directional RNA Library Prep kit (NEB) was used for library preparation under PCR cycle number of 14.
identifiers.namespace: GEO
identifiers.id: GSM8976947_r1