title: GSM8663885: SAT from early active rats (Control) rep3; Rattus norvegicus; RNA-Seq
library_construction_protocol: Approximately 80mg of adipose tissue was collected and stored in 2ml Precellys tubes (Bertin Instruments, CK28 - 2mL for adipose tissue), filled with the reagents according to the NZY Total RNA isolation kit protocol (NZY, MB1340). The tubes were immediately frozen by contact with dry ice and stored in a -80Cº freezer until further processing. Prior to further processing, the samples were homogenized (Precellys Evolution Homogenizer, Bertin Instruments, France) for 20 seconds at 4500RPM. After RNA isolation, samples were analyzed to determine the RIN score for RNA integrity (Agilent Technologies, 2100 Bioanalyzer). Only samples with a RIN score over 9 were used in all experiments. mRNA sequencing:  Purified RNA from VAT and SAT samples collected from EAC (n=6), EAE (n=6), LAC (n=6) and LAE (n=6) rats on P61, 24 hours after the last exercise session, were used to prepare polyA-selected Illumina short-read libraries following standard protocols at the Genomics Unit of the Centre for Genomic Regulation. Samples were sequenced on a HiSeq2500 and an average of 21 million 50-nt single-end reads were produced.
identifiers.namespace: GEO
identifiers.id: GSM8663885_r1