title: GSM8879101: LCL transformed under 1% O2 by EBV, replicate 2, H3K4me3, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: For RNA extraction, total RNAs were subsequently isolated using PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer's manual. An in-column DNA digestion step was included to remove any residual genomic DNA contamination. For chromatin samples, sample was prepared using the iDeal ChIP-seq kit for Histones (C01010059, HILOGIC Diagenode) following the sonication using Diagenode Pico. For RNA-seq library preparation, 500 ng total RNA was used for polyA mRNA-selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490, New England Biolabs, Inc.  Ipswich, MA), followed by library construction via NEBNext Ultra RNA Library Prep Kit for Illumina (#E7530, New England Biolabs, Inc., Ipswich, MA). For ChIP-seq libaray preparaion, DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (E7645S, NEB) and sequenced at the Tufts Genomics Core.
identifiers.namespace: GEO
identifiers.id: GSM8879101_r1