title: GSM8875780: P5_Setdb1_cKO_scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Dentate gyri originating from WT and SETDB1 cKO mice at P5 were collected in PBS on ice. The dissected DG was then digested using the Neural Tissue dissociation kit (P) (Miltenyi Biotec) according to the manufacturer's instructions. Approximately 20,000 cells were utilized as input for the 10X Genomics Chromium Controller, aiming for a targeted recovery of 10,000 cells per library. The libraries were then prepared employing the Chromium Single Cell 3' GEM, Library & Gel Bead Kit v3, strictly adhering to the manufacturer's instructions. Following the cDNA clean-up, the libraries were quantified using a BioAnalyzer then were sequenced on the Illumina NovaSeq 6000.
identifiers.namespace: GEO
identifiers.id: GSM8875780_r1