title: Illumina NovaSeq 6000 paired end sequencing
library_construction_protocol: L1 stage animals were allowed to exit diapause for 3 hours before a 30 minutes heat-shock at 34Â°C. Animals were then allowed to develop for 14 hours before collection and 4x wash in M9 buffer to remove as much bacteria as possible.  Animals were resuspended in 6.5 ml of M9, containing heat-inactivate OP50 E. coli, in a 50 ml conical tube. Animals were then incubated on a rotator for five hours, before collection by centrifugation and snap freezing in liquid nitrogen. Hi-C was performed according to the provider's manual (Arima Hi-C).  
identifiers.namespace: Institute for Cell Biology
identifiers.id: ena-EXPERIMENT-TAB-11-11-2025-09:09:59:778-79913