title: GSM8806698: MC38-LM3b-1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from the cells using TRIzol PolyA-tailed mRNA was enriched using Oligo(dT) magnetic beads. The RNA was fragmented and reverse transcribed with random N6 primers to synthesize cDNA. The double-stranded DNA was end-repaired, 5' phosphorylated, and ligated with a connector. The ligation products were PCR amplified, heat-denatured, and circularized using a bridging primer to generate a single-stranded circular DNA library.
identifiers.namespace: GEO
identifiers.id: GSM8806698_r1