title: Hybridization capture sequencing data of marine sedimentary ancient DNA from Northern Greenland
design: All libraries were processed using a custom myBaits hybridization capture panel following the High Sensitivity protocol of the myBaits User Manual (v 5.02) with the following additional modifications: the input mass of each indexed library into each capture reaction was 154 ng. One round of capture was performed and the input volume of baits and water in the hybridization mix was adjusted to 1.1 L and 4.4 L, respectively. Information on library preparation: a subset of samples (library names starting with Lib006) was processed using the semi-automated ancient environmental DNA pipeline operated by the GeoGenetics Sequencing Core facility at Globe Institute. For these samples, 0.35 g of sediment were subsampled and extracted using the Qiagen MagAttract Power Soil Pro kit, with modifications. After extractions, DNA concentrations were quantified by qPCR and a fixed number of cycles for the library build was determined (17 cycles). The libraries were prepared following the double-stranded protocol (Kircher et al., 2012) and unique 10-base pair motifs were used for double-indexing. The amplified libraries were purified and size selected with MagBio beads, targeting 60-600 base pairs (bp; with a 1.6x ratio). The remaining samples (library names starting with SCR) were processed manually from 1 - 14 ng of DNA (as determined by Qubit dsDNA HS assay) following the Santa Cruz Reaction protocol (Kapp et al., 2021). For the indexing, two unique 6-bp motifs were used for each library, to minimize the risk of cross-contamination during pooling for sequencing.
identifiers.namespace: SUB15013351
identifiers.id: cap47_Lib006_056