title: GSM8770263: 76_SFMC_TCR, CD45RO+ CD8+ PD-1+ TIGIT+ CD127-; Homo sapiens; RNA-Seq
library_construction_protocol: CD45RO+ CD8+ T cells were FACS sorted. Single cells were encapsulated in Gel-beads in emulsion (GEMs) using 5' gene expression library construction (10X Genomics). V(D)J amplification from cDNA aand V(D)J library construction were performed with Chromium Next GEM Single Cell 5' Reagent Kits v2 (10x Genomics) following manufacturer's protocols. Both 5' gene expression and V(D)J enriched libraries were quantified and evaluated via bioanalyzer high sensitivity chip (Agilent) prior to sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8770263_r1