title: Nanopore long-read genome sequencing of the IUCN Red List fungus Fomitopsis officinalis strain CBS 600.82
design: Genomic DNA was extracted using the WGS-CTAB method as described by Munoz et al (PMID 31734799). Quality and quantity was measured by Qubit Broad Range and TapeStation Genomic Screen. A starting amount of 650ng was used as input for the SQK-LSK114 library preparation kit of ONT. FASTQ data was collected after raw data basecalling in super accuracy mode. Reads with a Q10 or higher were collected and filtered with chopper (PMID 37171891) for fragments longer than 1100bp and a Q equal or higher than 20, thereafter 50bp of head and tail sequence were removed.
identifiers.namespace: SUB15167449
identifiers.id: CBS600.82v1