title: GSM8558105: Ad-NNMT-H3K4me3; Homo sapiens; Bisulfite-Seq
library_construction_protocol: After transfection, cells were harvested, and protein was extracted using lysis buffer suitable for subsequent Cut&Tag analysis with H3K4me3 and H3K27me3 antibodies. DNA was extracted following the standard protocols for methylation sequencing. Methylation sequencing libraries were prepared using a standard bisulfite conversion method, followed by adapter ligation and PCR amplification, optimized for paired-end sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8558105_r1