title: 16S rRNA gene amplicon sequencing data of adult female mus musculus faeces
design: Caecal content samples were snap frozen and stored at 80 C after collection. Bacterial DNA was isolated from the caecal contents using a DNeasy PowerSoil kit (Qiagen, Hilden, Germany) following the manufacturers instructions. DNA concentration and integrity were measured by a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and agarose gel electrophoresis, respectively. PCR amplification of the V3-V4 hypervariable regions of the bacterial 16S rRNA gene was carried out in a 25 l reaction using universal primer pairs (343F: 5-TACGGRAGGCAGCAG-3; 798R: 5-AGGGTATCTAATCCT-4). The reverse primer contained a sample barcode and both primers were connected with an Illumina sequencing adapter.
identifiers.namespace: SUB15672902
identifiers.id: Mouse-Control-02