title: GSM8840085: upf1_2; Naumovozyma castellii; ncRNA-Seq
library_construction_protocol: Total RNA was extracted using standard hot phenol procedure as described (PMID: 39535701) . Extracted RNA was ethanol-precipitated, resuspended in nuclease-free H2O (Ambion) and quantified using a NanoDrop 2000c spectrophotometer and/or a Qubit fluorometer with the Qubit RNA HS Assay kit (Life Technologies). For each condition, small RNA-seq analysis was performed from two biological replicates. Briefly, 25 µg of total RNA were mixed with 1 μg of total RNA from the YAM2400 (WT) strain of S. pombe; the 22–23-nt small RNAs derived from S. pombe centromeric regions are used as RNA spike-in to normalize small RNA-seq signals. Libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs), following manufacturer's instructions, starting from 50-100 ng of small RNAs (<80 nt) purified on 15% TBE–urea polyacrylamide gels.
identifiers.namespace: GEO
identifiers.id: GSM8840085_r1