title: RNA-seq of Chaetoceros simplex CCMP200 under heat stress
design: Total RNA was extracted from filters using Macherey-Nagel NucleoMag RNA kit (Macherey-Nagel, Dren, Germany). Cleared lysate was loaded into a 96 deep-well plate and put on an epMotion 5075 TMX liquid handler (Eppendorf, Hamburg, Germany) to complete the RNA extraction following the Macherey-Nagel standard protocol. RNA quality was analyzed on a 2200 TapeStation System with Agilent RNA ScreenTape System (Agilent Technologies, Santa Clara, CA, USA) and quantified using Quant-iT RiboGreen RNA Assay Kit (ThermoFisher, Waltham, MA, USA). PolyA enrichment mRNA transcriptome was constructed using 400ng-1ug of total RNA as input into the Illumina TruSeq RNA Sample Preparation Kit (Illumina, San Diego, CA, USA), following the manufactures Low-Throughput protocol. Library quality was analyzed on a 2200 TapeStation System with Agilent High Sensitivity D1000 ScreenTape System (Agilent Technologies). Resulting libraries were subjected to paired-end sequencing via Illumina HiSeq4000.
identifiers.namespace: SUB14774808
identifiers.id: Chaet 200-2-31