title: DNA-seq of Botrytis cinerea strain KY-1V1
design: To determine whether retroplasmids were integrated into the Botrytis cinerea genome, strains KY-1, KY-1V1, and B05.10 were resequenced. Briefly, genomic DNA was extracted from mycelium and fragmented enzymatically. The fragments were then end-repaired and A-tailed. After the ligation of sequencing adapters, size selection was performed using magnetic beads. The libraries were amplified by PCR, and the PCR products were assessed for concentration and size distribution. Finally, the libraries were pooled according to the target data output and sequenced on the NovaSeq 6000 platform.
identifiers.namespace: SUB15770328
identifiers.id: KY-1V1