title: RADseq genotyping by Floragenex using PstI RE and Illumina sequencing
design: DNA extraction by tissues grinding in mortar and pestle with liquid nitrogen, proteinase K and RNA digestions; deproteinization with phenol:chloroform:isoamyl alcohol (25:24:1) pH 8, followed by chloroform:isoamyl alcohol (24:1), and precipitation by isopropanol and 70% ethanol. DNA resuspension in ultrapure water. Restriction enzyme digestion by PstI. Size selection of the amplified P1P2 fragments to 300-500 bp (non-overlapping PE reads) using beads. Sp300 Cycle, 2x150 bp. Sequencing 95 samples per flow cell.
identifiers.namespace: SUB14849336
identifiers.id: plate 2_samples 96 to 190