title: 16S rRNA gene sequencing of angus heifer rumenal samples
design: Rumen samples centrifuged at 4,000  g at 4 C for 15 min, supernatant discarded, and pellet resuspended in 0.5 ml. DNA extraction was performed with Qiagen PowerMag DNA Isolation Kit and microbial 16S rRNA gene amplicon sequences were obtained using the following standard protocols (Weinroth et al., 2022) Q5 High-Fidelity DNA Polymerase (New England Biolabs, Inc., Ipswich, MA, USA) using 27F (Muyzer et al., 1993) and 534R (Lane, 1991) primers modified for Illumina sequencing. Amplicons were purified with AMPure XP bead (Beckman Coulter, Brea, CA, USA), followed by quantification using PicoGreen dsDNA quantification assay. Final pooled amplicon products were sequenced on the MiSeq Platform (Illumina, San Diego, CA, USA) according to standard protocols using a 1  300 v3 kit
identifiers.namespace: SUB14804309
identifiers.id: d83_1247