title: GSM8584547: Tumor 1; Homo sapiens; RNA-Seq
library_construction_protocol: RNA from fresh patient tissue samples was extracted using Trizol reagent, and total RNA quality and quantity were assessed using Agilent 4150 Tape Station system and Qubit 4 Fluorometer. The rRNA was depleted using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat), cat. No. #E6310X by following the manufacturer's protocol. RNA-Seq libraries were prepared with rRNA depleted sample using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (E7770L), according to manufacturer's protocols, and quality and quantity were assessed using Agilent 4150 TapeStation system, and Qubit 4 Fluorometer.
identifiers.namespace: GEO
identifiers.id: GSM8584547_r1