title: 5.23.FC Sink P-trap water 16S rRNA PacBio library
design: DNA per sample were amplified using the PacBio Kinnex 16S kit (PacBio) with Phusion Plus PCR Master Mix and the universal 27F/1492R primer pair; each at a final concentration of 0.3 M. Amplicons pooled without cleanup in volumes (120 l per reaction), based on gel band intensity, and purified and concentrated using 1.1 SMRTbell cleanup beads. The pooled library was eluted in 50 l of Low TE buffer. Kinnex PCR, size selection, and final cleanup were performed according to the manufacturers protocol.
identifiers.namespace: SUB15752759
identifiers.id: 5.23.FC_lib1