title: GSM9245282: Control Saline Mouse 3; Mus musculus; RNA-Seq
library_construction_protocol: Hippocampal tissue was manually dissociated on ice, then enzymatically dissociated using the Neural Tissue Dissociation Kit (Miltenyi, 130-092-628) per manufacturer instructions. Myelin was removed with Myelin Removal Beads (Miltenyi, 130-096-433). Cells were washed in FACS buffer (HBSS + 1% BSA) and stained in 100 uL FACS buffer with Live/Dead Fixable dye (1:500, Life Technologies L34964), FITC anti-mouse CD45 (1:100, BD 30-F11), and PE anti-mouse CD11b (1:100, BD M1/70) for 30 min at 4°C in the dark. After washing, cells were sorted on a BD FACSAria III. Single-cell cDNA libraries were prepared according to the manufacturer's instructions (Next GEM Single Cell 3′GEM v.3.1 protocol, 10x Genomics). Libraries were sequenced on a NextSeq2000 to a median read depth of ~38,000 reads per cell using the recommended read format (28x10x10x90bp).
identifiers.namespace: GEO
identifiers.id: GSM9245282_r1