title: GSM8806240: colon carcinoma MC38 tumor from Keap1 knockout mouse 1; Mus musculus; RNA-Seq
library_construction_protocol: Macrophage isolation from digested tumors: Anti-rat IgG Dynabeads were washed and incubated with rat anti-mouse F4/80 IgG2a antibodies and CD11b IgG2b antibodies. Single-cell suspensions from tumors were incubated with anti-CD45 coated Dynabeads on a shaker at 4°C for 30 min. After incubation, a positive selection of Dynabead-bound single-cell suspensions was performed on a DynaMag magnet with three washing steps, as suggested by the manufacturer's instructions. Single-cell suspensions were fixed following the demonstrated protocol Fixation of cells & Nuclei for Chromium Fixed RNA Profiling (10X Genomics, CG000478) and processed for long-term storage at -80°C. After storage, up to 2 Mio cells per sample were hybridized with unique single Mouse WTA probes using BC001-004 according to the demonstrated protocol Chromium Fixed RNA Profiling Reagent Kits for Multiplexed Samples (10X Genomics, CG000527).
identifiers.namespace: GEO
identifiers.id: GSM8806240_r1