title: GSM8806397: scRNA-seq of mouse tumor tissues 2; Mus musculus; RNA-Seq
library_construction_protocol: Tumors were dissociated in MACS C-tubes with Miltenyi Biotech tumor dissociation enzymes (#130-096-730). The dissociated cells were processed using ACK Lysis buffer (Gibco, #2537772), dead cell removal kit (Stem Cell Technologies #17899), and EasySep™ Mouse CD45 Positive Selection Kit (#18945). CD45+ cells were sorted into CD4+, CD8+, and CD11b+ populations, combined at equal ratios, and mixed 1:1 with live CD45⁻ cells before sequencing. 10X Genomics 3' single-cell RNA-seq (scRNA-seq) libraries were prepared following the manufacturer's instructions. Libraries were amplified by PCR and underwent quality control before sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8806397_r1