title: Amplicon sequence of soil metagenome: Grass1-2022
design: Amplicon fragments are PCR-amplified with Phusion Plus polymerase, then PCR is performed targetting the 16S V3-V4 region. PCR reactions are pooled to one plate, cleaned, normalized, to make one library. The library is quantified fluorometrically before sequencing.
identifiers.namespace: SUB15053029
identifiers.id: G1-2022