title: GSM9037125: ChIP for GFP in EGFP-GAF (EGFP-GAF) embryos staged at NC14M replicate ID= rep3; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: For ChIP experiments, embryos were crosslinked (Heptane/PBS/formaldehyde, 15 minutes) and sorted by stage prior to homogenization in RIPA buffer and sonication. Sonicated chromatin samples were subjected to immunoprecipiation with the indicated antibodies. ChIP libraries: following IP but prior to washes, antibody-chromatin complexes were tagmented by incubation with Illumina Tn5 (Nextera) reagent for 40 minutes at 37 degrees C. Following tagmentation, beads were washed and subjected to PCR with barcoding primers (Buenrostro, 2013).
identifiers.namespace: GEO
identifiers.id: GSM9037125_r1