title: RNA-Seq of Aedes aegypti antennae: Orlando 3
design: An average of 384 antennae/sample were dissected from female mosquitoes aged 5-7 DPE at ZT=0. RNA was extracted by phenol/chloroform extraction, using TRIzol reagent and substituting 1-bromo-3-chloropropane for chloroform. RNAseq was outsourced to Psomagen, Inc. The library was constructed with the Illumina Truseq stranded mRNA library prep kit. RNA was fragmented to 150bp long read length for paired-end sequencing. Sequencing was performed on the NovaSeq X Plus at 40x coverage. Data was delivered via an FTP link for download and analysis.
identifiers.namespace: SUB15235273
identifiers.id: Orlando3