title: HA_1_IP
design: The Cut & Tag experiment was performed by igenebook Co., Ltd (Wuhan, China). The Hieff NGS G-Type In-Situ DNA Binding Profiling Library Prep Kit for Illumina (Yeasen Biotech) was used to generate sequencing library according to manufacturers instructions. Briefly, 1106 LHX2-HA overexpressed DU145 cells were harvested and washed. The primary antibody of HA-tag (CST, #3724) was diluted 1:50 and incubated with samples overnight at 4 and incubated with the secondary lgG antibody (CST, #2729) at 4 for 2 hours. The sequencing library were conducted using an Illumina NovaSeq instrument. The raw reads were aligned to the reference genome (GRCh38). The BigWig files were obtained using the genomic Ranges R package.
identifiers.namespace: SUB14955724
identifiers.id: HA_1