title: GSM8701610: 1_H_H3K4me3; Homo sapiens; OTHER
library_construction_protocol: MCF10A cells (empty plasmid vector, or H2B variants expressing cells) were grown to 70-90% confluency. A total of 5 × 10⁵ cells were used per reaction. The cells were washed and subsequently incubated with the target antibody according to the protocol provided in the CUTANA CUT&RUN (version 3) kit. Nuclei bound to ConA beads were then sent to Fred Hutchinson Cancer Research Center (Seattle, WA) for genomic DNA extraction and sequencing. CUT&RUN seq libraries were prepared in Fred Hutch Cancer center Genomics core. The end repair, A-tailing, adapter ligation, post-ligation cleanup, library amplification, and post-amplification cleanup steps were carried out using the KAPA HyperPrep Kit (Kapa Biosystems, Wilmington, MA, USA), following the manufacturer's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8701610_r1