title: GSM8879215: SYF1187-HTGTS-day9-iNs-AraC-bait-chromosome15-B2M-replicate2; Homo sapiens; OTHER
library_construction_protocol: For LAM-HTGTS, genomic DNA was extracted using DNeasy® Blood & Tissue (QIAGEN; cat. No. 69504) following the manufacturer's instructions. For LAM-HTGTS, 10 µg genomic DNA from iNs were sonicated and subjected to LAM-PCR with a biotinylated primer. The ssDNA products are enriched with streptavidin magnetic beads enrichment and ligated to a bridge adaptor. Nested PCR and tagged PCR were used to amplify the translocation junctions and barcode for next-generation sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8879215_r1