title: GSM8741359: LX470, AT2-MM; Homo sapiens; RNA-Seq
library_construction_protocol: After aspirating media, ~1 ml TrypLE (#12605010, Gibco) was added to one well of a 6 well plate and BME domes were disrupted mechanically by pipetting up and down. The organoid suspension was thereafter collected into a 15 ml tube and incubated at 37C for 2-4 min. Upon a suspension of mostly single cells, 10 ml of cold washing media was added, and cells were spun down at 400 g for 5 min. Live cells (DAPI-/DRAQ5+) were subsequently sorted into PBS using a BD FACSAria Fusion. Cells from the same condition but equal donors were mixed in equal parts and 25,000 cells per condition were subjected to droplet-based scRNA-seq using the 10x Genomics platform Libraries were prepared using the 10x Genomics Chromium 3' Gene Expression solution v3 on a GEMX machine and sequenced on a NovaSeq6000 (Illumina)
identifiers.namespace: GEO
identifiers.id: GSM8741359_r1