title: PtoMIR6438a-OE1-3-RNA-seq (CRX657442; CRA010652)
library_construction_protocol: (1) RNA quantification and qualification: RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system.(2) Library preparation: First strand cDNA was synthesized using random hexamer primer and M-MuLV Reverse Transcriptase (RNaseH-). Second strand cDNA synthesis was subsequently performed using DNA Polymerase I and RNase H. Products were purified (AMPure XP system) and library quality was assessed on the Agilent Bioanalyzer 2100system.(3) Clustering and sequencing: The library preparations were sequenced on an Illumina NovaSeq 6000 platform and 150bp paired-end reads were generated.
identifiers.namespace: Beijing Forestry University
identifiers.id: DRX709285
identifiers.namespace: NGDC
identifiers.id: CRX657442