title: GSM9279102: Isolated Human Hepatocytes, HH002, PNPLA3-I148M; Homo sapiens; RNA-Seq
library_construction_protocol: total RNA was extracted from isolated primary human hepatocytes using RNeasy Plus Micro Kit (Qiagen). RNA integrity was assessed using the High Sensitivity RNA ScreenTape system on an Agilent 2200 TapeStation (Agilent). The SMART-Seq HT Kit (Takara Bio) was used to generate cDNA from 10 ng of total RNA, and the cDNA product was checked by an Agilent Fragment Analyzer system (Agilent) for quality control. The sequencing library was constructed by following the Illumina Nextera XT Sample Preparation Guide. One nanogram of input cDNA was tagmented and amplified using the Illumina Nextera XT kit. Equimolar amounts of each sample were finally pooled and sequenced on an Illumina Nextseq 500 system, using a paired-end 75-bp strategy
identifiers.namespace: GEO
identifiers.id: GSM9279102_r1