title: GSM8585608: RChIP_DMSO4h_rep2; Homo sapiens; ChIP-Seq
library_construction_protocol: Nuclei were isolated and sonicated using a Bioruptor Pico (Diagenode). RNaseH1Mut-V5 was immunoprecipitated, and the cross-linked DNA was reversed by overnight incubation at 65°C. After treatment with RNase A and proteinase K, DNA was recovered by ethanol precipitation. Phi29 DNA polymerase was used to synthesize second-strand DNA, followed by dA tailing and adaptor ligation. The library was amplified and sequenced with single-end 50 bp reads on the Illumina NextSeq 2000, generating approximately 50 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM8585608_r1