title: GSM8976051: Direct_Infection_KO_Activated_5; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen) following the manufacturer's instructions For 40dpi samples, RNA quality and quantity were assessed using TapeStation 4200 RNA tapes (Agilent) and RNA-seq libraries were prepared from 0.2-6.0ng total RNA using the SMART-seq v4 Ultra Low Input RNA Kit (Takara 634773). For all other samples, libraries were prepared from 15ng total RNA using the NEBNext Ultra DNA Library Prep kit for Illumina (NEB E7370L). Library quality control was then performed using TapeStation 4200 High-Sensitivity DNA tapes (Agilent). Dual-indexed libraries were pooled and sequenced on a NextSeq500 instrument (Illumina) for 40dpi samples or a NextSeq2000 instrument for all other samples (Illumina) 75 or 100 cycles, respectively, single-end, to an average sequencing depth of 14.1 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM8976051_r1