title: 16S rRNA-Seq Negative Control For Adult Persian Fallow Deer Buck Fecal Pellet Extraction
design: Approximately 250g of fecal material was placed into PowerBead Tubes containing 2.38mm metal beads, and DNA was extracted using the DNeasy PowerSoil Pro Kit. Two negative controls were included to monitor contamination. After extraction, the DNA was quantified and processed at the Microbial Analysis, Resources, and Services (MARS) facility at the University of Connecticut. DNA extracts, along with negative controls, were quantified using a Quant-iT PicoGreen kit. Bacterial and archaeal 16S rRNA sequences (V4 region) were amplified using primers 515F and 806R with Illumina adapters. Samples were amplified in triplicate, normalized, pooled, and cleaned with Omega Bio-Tek Mag-Bind Beads. Finally, sequencing was performed on a MiSeq platform using a v2 2x250 base pair kit.
identifiers.namespace: SUB14796110
identifiers.id: 22Control