title: GSM8644254: hepatocellular carcinoma tissue of HCC patient 1; Homo sapiens; RNA-Seq
library_construction_protocol: High quality samples are most important for small RNA sequencing projects. The recommended purified total RNA amount is 1~2ug. Alternatively, we offer to isolate RNA from your sample sources, including cultured cells, tissues, serum or plasma. Before the sequencing experiment, we check the integrity and quantity of each RNA sample using agarose gel electrophoresis and NanodropTM instrument. The RNA QC results are included in the service report. tRNA derived fragments (tRF & tiRNA) are heavily decorated by RNA modifications that interfere with small RNA-seq library construction. We do the following treatments before library preparation for total RNA samples: 3'-aminoacyl (charged) deacylation to 3'-OH for 3' adaptor ligation, 3' -cP (2' ,3' -cyclic phosphate) removal to 3' -OH for 3' adaptor ligation, 5' -OH (hydroxyl group) phosphorylation to 5' -P for 5' -adaptor ligation, m1A and m3C demethylation for efficient reverse transcription. Sequencing libraries are size-selected for the RNA biotypes to be sequenced using an automated gel cutter. The libraries are qualified and absolutely quantified using Agilent BioAnalyzer 2100. For standard small RNA sequencing on Illumina NextSeq instrument, the sequencing type is 50bp singleread.
identifiers.namespace: GEO
identifiers.id: GSM8644254_r1