title: GSM8739986: Rx-GFP WT-H3K27me3; Mus musculus; OTHER
library_construction_protocol: Day 4 WT and Mettl3KO ROs were harvested and trypsinized to single cell suspension. 2×106 cells were subjected for CUT&RUN experiment per antibody as previously described, A CUT&RUN kit (EpiCypher, 14-1048) was used as manufacture's protocol and the following antibodies were used: METTL3 antibody (Abcam, Ab195352), H3K4me3 antibody (Millipore, 07-473) and H3K27me3 antibody (ActiveMotif, 39155). The CUT&RUN-DNA were sent to University of Michigan DNA Sequencing Core for quality control, library preparation, and sequencing using Illumina NovaSeq S4 Libraries platform.
identifiers.namespace: GEO
identifiers.id: GSM8739986_r1