title: GSM8585510: HPFH1.1_ATAC; Homo sapiens; ATAC-seq
library_construction_protocol: Reticulocytes were sorted from primary blood draws through the gating strategy hCD45-/hCD71+/Thiazole Orange+ for library preparation. Undifferentiated (not sorted) HUDEP-2 cells were used for library preparation. Cells were processed using 10X Genomics reagents according to manufacturer's protocol. For reticulocytes, gel beads-in-emulsion (GEM) generation, cDNA amplification, and library construction were performed following the protocol of Chromium Next GEM Single Cell 3′ Reagent Kits v3.1 (Dual Index, 10x Genomics) with target recovery of 8,000–10,000 cells per library or sample. Libraries derived from HUDEP-2 cells were prepared following the 10X Genomics Single Cell Multiome ATAC + Gene Expression suggested protocol.
identifiers.namespace: GEO
identifiers.id: GSM8585510_r1