title: GSM8740726: validation and further samples; Homo sapiens; RNA-Seq
library_construction_protocol: For single cell RNA sequencing, cells were harvested using TrypLE, resuspended in PBS, filtered through 30 µm Celltrix filters, resuspended in cell freezing medium and stored at -80°C until further processing. Cryopreserved cells were quickly thawed in a 37°C waterbath, washed in cell culture medium and resuspended in 1xPBS supplemented with 10% fetal calf serum. Cells were counted using an automated cell counter TC20 (BioRad) and equimolar amounts of cells per condition were labelled with 10X Genomics CellPlex barcodes according to the manufacturer's protocol (CG000391_Rev B). Chromium Next GEM Single Cell 3' Reagent Kit v3.1 (CG000388_Rev B) and the Chromium Controller iX (10X Genomics) were used according to the manufacturer's protocol
identifiers.namespace: GEO
identifiers.id: GSM8740726_r1