title: TCR-sequencing of human: adult female immune cells
design: Peptides with demonstrated immunogenicity by ELISpot assay were used to stimulate PBMCs or irrelevant peptides as a control. On day 12, cells were harvested, and genomic DNA was extracted and purified from cells using the QIAGEN Blood and Tissue Kit , then TCRV_ CDR3 regions were amplified using the Adaptive Biotechnologies ImmunoSEQ kit then sequenced following manufacturer instructions
identifiers.namespace: NCI-phs002787
identifiers.id: BRC18_pre_medium_TCRB-lib1