title: Shallow metagenomic shotgun sequencing data of marine sedimentary ancient DNA from Northern Greenland
design: All samples were processed using the semi-automated ancient environmental DNA pipeline operated by the GeoGenetics Sequencing Core facility at Globe Institute. For these samples, 0.35 g of sediment were subsampled and extracted using the Qiagen MagAttract Power Soil Pro kit, with modifications. After extractions, DNA concentrations were quantified by qPCR and a fixed number of cycles for the library build was determined (17 cycles). The libraries were prepared following the double-stranded protocol (Kircher et al., 2012) and unique 10-base pair motifs were used for double-indexing. The amplified libraries were purified and size selected with MagBio beads, targeting 60-600 base pairs (bp; with a 1.6x ratio).
identifiers.namespace: SUB15013709
identifiers.id: eDNALib006_045