title: GSM8740728: input_D, 1~2 year-old, rep1; Ovis aries; RNA-Seq
library_construction_protocol: Total RNA was extracted from ovarian tissues using Trizol lysis (Invitrogen, CA, USA). Additionally, genomic DNA was removed using the rDNAIR-free enzyme (TaKara). The genomic DNA was extracted and analyzed using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA) and a NanoDrop 2000 (Thermo Fisher Scientific, Waltham, MA, USA) to verify RNA quality. Qualified RNA used for sequencing library construction had an OD 260/280 ratio of ≥ 1.8, a RIN of ≥ 8, and exhibited significantly brighter 28S bands compared to 18S bands. The RNA concentration of all samples was 200 ng/μL. Quality-qualified RNA was used for library construction according to the TruSeq stranded total RNA kit (Illumina, San Diego, CA).High-throughput sequencing was performed using the Illumina NovaSeq 6000 sequencing platform (OE Biotech Co., Ltd., Shanghai, China).
identifiers.namespace: GEO
identifiers.id: GSM8740728_r1