title: GSM8701571: rpoC-G1122D-rpoB-H482Q-R-1; Bacillus subtilis; RNA-Seq
library_construction_protocol: RNA was isolated from 1.5 mL of cells grown till ~0.4 OD600nm using QIAGEN RNeasy Kit (Cat. No. 74106). Genomic DNA contamination was removed by treating RNA with TURBO DNA-free kit from Invitrogen (Cat. No. AM1907) UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit. Each library was quantified with Qubit (dsDNA HS kit; Thermo Fisher) and the size distribution was determined with Fragment Analyzer prior to pooling
identifiers.namespace: GEO
identifiers.id: GSM8701571_r1