title: GSM8739997: hPSCs_OTX2-KO-1; Homo sapiens; ATAC-seq
library_construction_protocol: Fresh cells were collected and washed with 500 μl wash buffer and centrifuged at 500×g for 5 min at 4℃. Cell pellets were washed with 50 μl pre-cooled TW Buffer for twice.Re-suspended the cell pellets  with 50 μl precooled lysis buffer, placed on ice for 5 min, centrifuged at 500×g for 10 min at 4°C, and the supernatant was discarded. The ATAC-seq library was performed according to the manual of the Hyperactive ATAC-Seq Library Prep Kit for Illumina (TD711, Vazyme Biotech). 1 × 105 cells were used as starting material for every sample. Libraries were amplified using 12 cycles on the thermocysler. Post amplification libraries were size select at 250-450bp in length using DNA Clean Beads  (N411, Vazyme Biotech). Libraries were validated using the dsDNA HS Assay Kit (EQ121, Vazyme Biotech).
identifiers.namespace: GEO
identifiers.id: GSM8739997_r1