title: Hi-C of XiangQinglan: young leaves
design: The crosslinked DNA was fixed in the cell nucleus by formaldehyde, and the restriction enzyme Mbo I was used to digest the DNA. The ends were filled and marked with biotin, then ligated by DNA ligase to form a loop. The crosslinked fragments were obtained via protease digestion and purification. After being interrupted again using ultrasound, the crosslinked DNA with the size of 300~500bp marked with biotin was enriched for the Hi-C library construction.
identifiers.namespace: SUB15751359
identifiers.id: V350220322_L01_124