title: GSM8976551: U2OS cells, hour 0, replicate 2; Homo sapiens; RNA-Seq
library_construction_protocol: At assay endpoint cells were trypsinized, counted using a hemacytometer, pelleted, washed with PBS, and moved immediately to RNA extraction. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, 74134) according to manufacturer protocol. To facilitate absolute RNA quantification, polyadenylated ERCC standards (Thermo Fisher Scientific, 4456740) were diluted 1:100 in nuclease-free water, and added to each sample at 1 µL/100,000 cells. ERCC spike-ins were added immediately following lysis of cells in RLT-plus buffer, and prior to all subsequent total RNA extraction steps using the RNeasy Plus kit. mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490). RNAseq libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit using the NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7765 and E7600).
identifiers.namespace: GEO
identifiers.id: GSM8976551_r1