title: dRNA sequencing of molm13 cell line transduced with scramble shRNA
design: MOLM13 cells underwent transduction using lentiviruses via spinfection at a speed of 1400 revolutions per minute (rpm) for a duration of 1 hour at room temperature. Subsequently, the cells were seeded in RPMI supplemented with 10% fetal bovine serum (FBS) at a density of 0.5x106 cells/ml. To enable puromycin selection, a concentration of 3 g/ml puromycin was introduced to the transduced cells at 24 hours post-transduction. After an approximate period of 48-56 hours post-transduction, during which a puromycin selection phase spanning approximately 24-32 hours was implemented, cells were harvested. The selected cells were then gathered for subsequent Western blot analyses and nanopore direct RNA sequencing procedures. transduced with scramble shRNA as experimental control
identifiers.namespace: SUB15062971
identifiers.id: F112877f5