title: GSM8840093: WT_CHX_2; Naumovozyma castellii; OTHER
library_construction_protocol: Cells were lysed and ribosome-protected fragments (RPFs) were prepared as previously described (Andjus et al., 2024), starting from 20 A260 units of extracts. After rRNA depletion, RPFs were treated with 5 units of Antarctic phosphatase (New England Biolabs), and then with 10 units of T4 polynucleotide kinase (New England Biolabs) in the presence of 1 mM ATP. Phosphorylated RPFs were purified using the RNA Clean & Concentrator-5 kit (Zymo Research) following manufacturer's instructions, and eluted in 15 µL of nuclease-free H2O. Ribo-seq libraries were constructed using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs), following manufacturer's instructions. Amplified libraries were purified using AMPure XP beads (Beckman Coulter), and then on 6% Novex TBE gel (Invitrogen).
identifiers.namespace: GEO
identifiers.id: GSM8840093_r1