title: 16S rRNA gene amplicons of rhizosphere microbiota of faba bean   biological replicate 4 (forward)
design: Briefly, total genomic DNA was extracted using the Qia- gen QIAamp Fast Stool Mini Kit (Qiagen, Shanghai, China) according to the manufacturers protocol. DNA concentration was determined using a Nano-Drop 2000 spectrophotometer. The V3-V4 hypervariable regions of the bacterial 16S rRNA gene were amplified in triplicate using the primer pair 338F (5-ACTCCTACGGGAGGCAGCAG-3) and barcoded 806R (5- GGACTACHVGGGTWTCTAAT-3) under the following PCR cycling conditions: initial denaturation at 94 C for 3 min followed by 5 cycles of denaturing at 94 C for 10 s, annealing at 55 C for 15 s, and extension at 72 C for 30 s before a final extension at 72 C for 5 min. PCR products were visualized on 2% agarose gel (in TAE buffer) containing ethidium bromide and purified with a DNA gel extraction kit (Axygen, Shanghai, China).
identifiers.namespace: SUB15217354
identifiers.id: WC_4.515F_907R.R1.raw.fastq