title: 16S rRNA sequencing of Abies pindrow: Seed microbiome
design: DNA was extracted using the Qiagen DNeasy Plant Mini Kit. The ITS2 region was amplified with primers ITS3mix and ITS4ngs, then barcoded, purified, and quantified. Paired-end sequencing (2300 bp) was performed on an Illumina MiSeq platform. Raw reads were quality-checked, with only high-quality data used.
identifiers.namespace: SUB15753691
identifiers.id: LIB_SF2_ITS