title: RNA-seq of H1 human embryonic stem cells engineered with CRISPRi machinery at CLYBL using Cas9, replicate 1
design: Stem cells are transfected with effector and donor plasmids and selected for 2-3 weeks until bulk stable cell lines are established. Next, RNA is extracted using Trizol and purified with phenol chloroform precipitation. Roche/KAPA mRNA enrichment followed by HyperPrep Library Preparation Kit.
identifiers.namespace: SUB15587463
identifiers.id: 20250310_Stem_H1_CLYBL_5