title: GSM8838661: IMR-90, Sen-siBRD4, ATAC-seq (single-end), rep2; Homo sapiens; ATAC-seq
library_construction_protocol: Cells were used for ATAC-seq following the manufacturer's instructions (Active Motif). Cells were pelleted and washed with 100 μL of ice-cold PBS. Cells were lysed with 100 μL of ATAC Lysis Buffer on ice for 3 min and were spun down at 500 g for 10 min at 4°C. The nuclei were incubated in 50 μL of Tagmentation Master Mix at 37°C for 30 min. Accessible DNA regions in nuclei were probed with Tn5 Transposase, which inserts sequencing adapters into open regions. The tagged DNA was purified, PCR-amplified, and sequenced using the Illumina NextSeq 500/550 system (Illumina), according to the manufacturer's protocol. The libraries were subjected to 75-bp single-end or 35-bp paired-end sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8838661_r1