title: GSM8584207: NoDox_d4_rep3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA from flash frozen samples was extracted using ISOLATE II RNA Mini Kit as per manufacturer recommendations (Bioline). In short, samples were thawed on ice, lysed and passed through a column purification process and DNase treatment. Precipitated RNA was eluted into RNase-free water. RNA concentration, DNA and ethanol contamination were measured using the NanoDrop (Thermo Fisher) and adjusted using RNase-free water as required. RNA-seq libraries were prepared from extracted RNA by Genewiz (Azenta, China) using the NEBNext Ultra II RNA Library Preparation Kit (NEB, E7770) as per manufacturer protocol and 150 bp paired-end sequenced on the HiSeq 4000 (Illumina). 150 bp paired-end reads were trimmed using Trim Galore (Felix 2021)  in paired mode `--paired` keeping reads with at least 50 bp `--length 50`.
identifiers.namespace: GEO
identifiers.id: GSM8584207_r1