title: GSM8839594: 107_22_null_fetal_3D_culture; Mus musculus; RNA-Seq
library_construction_protocol: For adult- crypt enriched fractions were used to make single-cell suspensions, FACS sorted and run on 10x Controller. For fetal 3D cultures- single cell suspensions were fixed and processed using 10x Genomics Flex technology. The samples were run on a Chromium X instrument. For samples from adult mice, library construction was according to the manufacturer's protocol (single cell 3´v3.1 protocol, 10x Genomics).  Libraries from fixed fetal cells from 3D cultures were prepared using Next GEM single cell fixed RNA hybridization and library kit (catalog nr: 1000415) and according to manufacturer's protocol (10x Genomics).
identifiers.namespace: GEO
identifiers.id: GSM8839594_r1