title: 16S sequencing of antibiotic-treated mice
design: Fresh fecal samples were collected from individual mice prior to antibiotic administration and immediately prior to infection and frozen for storage at -20C. DNA was isolated from fecal samples using the PowerSoil DNA isolation kit (MO BIO Laboratories). For each sample, the V4 region of the 16S rRNA gene was amplified and sequenced using the Illumina MiSeq Personal Sequencing platform. Paired-end sequences were imported into QIIME2 (version 2022.2).
identifiers.namespace: SUB15769893
identifiers.id: 221-1_post_abx