title: GSM8806097: WT-whole heart-2 replicates; Mus musculus; OTHER
library_construction_protocol: The pups' hearts were extracted at P1, sliced, fixed in 4% PFA and embedded in Parrafin Using 5 µm tissue sections on Visium HD Gene Expression slides (10X genomics), libraries were formed with Spatial Gene Expression Reagent Kits for FFPE tissues. These sections, after H&E staining, were imaged at 40X on a Leica Aperio ImageScope and processed for Visium libraries with CytAssist, targeting a slide size of 6.5 x 6.5 mm. Capture Areas were arrayed with a continuous lawn of oligonucleotides organized into millions of 2 x 2 µm barcoded squares without gaps. After checking the quality control, cDNA libraries were sequenced on the Illumina NovaSeq 6000, aiming for >275 million read pairs for capture areas covered fully by tissue
identifiers.namespace: GEO
identifiers.id: GSM8806097_r1