title: GSM8840044: Tumor under anti-Cd47ab treatment;  Tumor under a combination therapy of anti-Cd47ab and Kras-inhibitor; Mus musculus; OTHER
library_construction_protocol: Formalin-fixed paraffin-embedded (FFPE) tissue samples were sectioned at a thickness of 5 µm using a microtome and mounted onto Visium HD Spatial Gene Expression slides (10x Genomics, Inc., Pleasanton, CA, USA). Tissue sections were subjected to hematoxylin and eosin (H&E) staining following the manufacturer's protocol. Images were acquired at 20× magnification using a high-resolution bright-field microscope (VS-200, Evident Corp., Tokyo, Japan) and used for subsequent spatial alignment. Hybridization of mRNA from the tissue sections to barcoded oligonucleotide probes on the slide surface was performed using CytAssist (10x Genomics), followed by library preparation, including cDNA synthesis and amplification, fragmentation, and adapter ligation, according to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8840044_r1