title: GSM8877575: Br5844_Nuc_SETD1A_Atlas_8G; Homo sapiens; OTHER
library_construction_protocol: Nuclei were isolated from human prenatal frontal lobes (110-170 mg tissues). The nuclear pellets were resuspended gently in nuclei storage buffer (NSB) (167 mM sucrose, 5 mM CaCl2, 3 mM Mg(Ac)2, Tris-HCl (pH 7.5) and 1× protease inhibitor) and incubated on ice until the pellet is loosened. NPCs were dissociated with Accutase at 37°C for 2 min. Four-week-old neurons were dissociated with Papain (Worthington Biochemical) dissolved in 1:1 solution of Accutase and 1× PBS at 37°C for 30 min. Dissociated neurons were then washed with Neurobasal medium supplemented with 1× GlutaMax and 2,000 U/mL DNase I (Worthington Biochemical). 1× 105 nuclei or cells for histone marks and 1× 106 nuclei or cells for SETD1A were used. Libraries were sequenced on HiSeq2500 (Illumina) with a read length of 50-bp paired-end by Psomagen.
identifiers.namespace: GEO
identifiers.id: GSM8877575_r1