title: small-RNA-seq of IgG immunoprecipitated RNA from GFP-dsRNA injected WT anemones 24-hours old gastrula replicate3
design: Nematostella zygotes were injected with dsRNA carrying the 3'-half of eGFP sequence then frozen in liquid nitrogen after 24 hours. NveAGO2 immunoprecipitation was performed as previously described (Fridrich et al, 2020) with rabbit IgG as control. RNA was extracted with Tri-reagent (Merck Millipore) according to manufacturer's protocol. RNA was size selected for 18-30 nucleotides long sequences on urea-PAGE gel (Bio-Rad) and libraries were prepared with NEBNext Multiplex Small RNA Library Prep Set for Illumina kit (New England Biolabs) with modified protocol adapted to low RNA input (Plotnikova et al, 2019)
identifiers.namespace: SUB15724878
identifiers.id: IGG3