title: GSM8902235: BEAS-2B cells, αC, biol rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from treated cells using the PureLink™ RNA Mini Kit (Thermo Fisher) following the manufacturer's instructions. A total of 2 µg of purified RNA was subsequently used for library preparation for RNA sequencing. Following mRNA enrichment and fragmentation, first- and second-strand cDNA synthesis was performed using random primers, reverse transcriptase, and DNA polymerase I. Sequencing adaptors were then ligated to both ends of the cDNA fragments. The resulting libraries were PCR-amplified, purified, and quality-checked before sequencing.
identifiers.namespace: GEO
identifiers.id: GSM8902235_r1