title: GSM8659129: contralateral_muralcells_sample3; Mus musculus; RNA-Seq
library_construction_protocol: Nerve cells were dissociated from Pdgfrb-CreERT2-tdtomato mice using the methods described in the associated paper. Pdgfrb+ and Pdgfrb+/CD146+ populations were sorted using FACS into 4µl SMART-seq2 lysis buffer. We sorted a maximum of 100 cells per tube, so as to not overload the solution. Sequencing libraries were then prepared in batch controlled fashion using the Smart-seq2 protocol described by Picelli and colleagues (Picelli et al., 2014). Sequencing was performed on these by Novogene Europe.
identifiers.namespace: GEO
identifiers.id: GSM8659129_r1