title: GSM8585749: APP-2-A2-GEX; Mus musculus; OTHER
library_construction_protocol: APP23tg/APP23wt mice were sacrificed using Ketamin/Rompun overdose intraperitoneally, brains were isolated respectively, followed by 50 ug/ml Liberase (Sigma) digestion at 37ºC for 8 min and mashing through a 100 and 70 µm strainer (Miltenyi) to generate a single cell suspension. Subsequently, Myelin removal was performed using a 30% continuous Percoll (GE Healthcare) gradient and centrifugation at 2700 rpm. library preparation were carried out on the Chromium platform (10x Genomics) with the Single Cell 5ʹ reagent v2 kit (10x Genomics) according to the manufacturer's protocol
identifiers.namespace: GEO
identifiers.id: GSM8585749_r1