title: GSM8770267: EVsFBMSC; Macaca mulatta; RNA-Seq
library_construction_protocol: For single-cell RNA sequencing (scRNA-seq), bone marrow samples from aged rhesus macaques were collected from each group (vehicle, EVsF-BMSCs and EVsABPCs) at 20 weeks post treatment. Specifically, one rhesus macaque per group was selected for bone marrow aspiration under anesthesia. A 10x Chromium Controller (10x Genomics) was utilized to capture individual cells within droplet emulsions, targeting an output of 10,000 cells for each sample. Following the manufacturer's protocol, a scRNA-seq library was constructed using a Chromium single-cell 3' gel bead and library V3 kit in accordance with the manufacturer's guidelines. The libraries were pooled and sequenced using a NovaSeq 6000 sequencing system (Illumina), strictly adhering to the manufacturer's instructions.
identifiers.namespace: GEO
identifiers.id: GSM8770267_r1