title: GSM8585404: Zfp36l1/Zfp36l2 dKO 2; Mus musculus; RNA-Seq
library_construction_protocol: On day 7, 0.5x10^6 CD19+ B220hi CD138- TACI- iGCB cells were FACS-sorted, washed twice with ice-cold PBS and cell pellets were snap frozen in liquid nitrogen. RNA was extracted using the RNeasy Micro Kit (Qiagen) and its quality was assessed on a 2100 Bioanalyser (Agilent). RNA integrity numbers > 8 of total RNA were used to generate cDNA. Opposing strand-specific RNA-sequencing libraries were generated using the NEB PolyA kit (E7490) and the NEB Ultra II stranded kit (E7760) with NEB dual index, according to the manufacturer's instructions, and sequenced on an Illumina NovaSeq 6000 using 50bp paired-end reads.
identifiers.namespace: GEO
identifiers.id: GSM8585404_r1