title: RNA-seq of B. theta in vitro cultures under different stress conditions
design: In vitro and in vivo samples stored at -80 C were thawed on ice, and their RNA was extracted using the RNeasy Mini Kit (Qiagen 74104) or RNeasy PowerMicrobiome Kit (Qiagen 26000-50), respectively, as per manufacturer instructions. RNA samples were depleted of gDNA using the TURBO DNA-freeTM Kit (Invitrogen AM1907). RNA quantity and quality were verified by Qubit quantification and Agilent BioAnalyzer prior to NEBNext Ultra II RNA library preparation. Sequencing was performed on the Illumina NextSeq2000 sequencer using a P3 flow cell and 50 bp single end reads.
identifiers.namespace: SUB15755082
identifiers.id: S34_R1_001