title: scRNA-seq of hemocytes from Ciona robusta (blood1)
design: Hemolymph of ascidians (Ciona robusta) was collected from the pericardium. Two samples were obtained from two individual animals and used to generate separate libraries. An aliquot of 100 L of hemolymph was diluted with 900 L of hemocyte buffer (11 mM KCl, 43 mM Tris-HCl, pH 7.5, 0.475 M NaCl; osmolarity ~975 mOsm kg) and gently mixed by pipetting. The cell suspension was centrifuged at 300  g for 5 min at 15 C. After washing twice with hemocyte buffer, the hemocytes were resuspended in an equal volume of fresh buffer. Trypan blue staining confirmed >90% cell viability in all samples. Single-cell suspensions were processed following the manufacturers protocol for the Chromium Next GEM Single Cell 3 Reagent Kit v3.1 (Dual Index) (10x Genomics). The target cell recovery was set at 5,000 cells per sample. Encapsulation and barcoding were performed using the Chromium Controller (10x Genomics). Libraries were constructed according to the manufacturers instructions.
identifiers.namespace: SUB15514999
identifiers.id: CionaBlood1_S1