title: GSM8584380: 105379-001-016; Homo sapiens; RNA-Seq
library_construction_protocol: Isolated tissues were dissolved in 500 µL Trisure (BIO-38032, Bioline). Subsequently, 100 µL CHCl3 was added to the tube and the solution was centrifuged (15 min, 11,000 rcf, 4 ⁰C). The aqueous layer was carefully removed and 500µL was added to the same tube for an additional extraction. After combining the two aqueous layers 1 equal volume 70% EtOH was added and the solution was added to an RNAeasy column (QIAgen). The RNA was washed with RW1 buffer, after which any DNA contamination was removed by incubation with DNase (15 min, RT). After this the column was washed in subsequent steps with RW1 buffer, then with RPE buffer and lastly with 80% EtOH. The column was air dried for 5 minutes before eluting the RNA in 16 µL RNAse free water. 1 µL of the extracted RNA was used for analysis on a Denovix DS-11 spectrofotometer to analyze the purity and concentration. NEBNext Ultra II Directional RNA Library Prep Kit for Illumina was used to process the sample(s).
identifiers.namespace: GEO
identifiers.id: GSM8584380_r1