title: The V3 and V4 region genes of 16SrRNA of ileal microorganisms in male broilers
design: Microbial genomic DNA from the ileum was extracted using the FastPure Stool DNA Isolation Kit (MJYH, Shanghai, China), and evaluated for quality via 1% agarose gel electrophoresis. The V3-V4 region of the bacterial 16S rRNA gene was amplified using universal primers (338F: 5'-ACTCCTACGGGAGGCAGCAG-3'; 806R: 5'-GGACTACHVGGGTWTCTAAT-3'). Following magnetic bead purification, the amplified products were fluorescently quantified with the Qubit 4.0 (Thermo Fisher Scientific, USA). High-throughput sequencing was then performed on the Illumina Nextseq2000 platform (Illumina, San Diego, USA). Subsequent bioinformatics analysis was conducted using the Majorbio Cloud platform (https://cloud.majorbio.com). Sequencing procedures were carried out by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China).
identifiers.namespace: SUB15012554
identifiers.id: CM3