title: 16S rRNA of microbial community in seawater: vSM
design: 16S rRNA and rDNA sequencing was conducted using primers 515F and 926R (Walters et al., 2015) as further described by Givati et al. (2024). In short, amplicons were generated using a two-stage PCR protocol, and libraries were sequenced on an Illumina MiSeq platform (V3 chemistry, 2  300 bp reads) with a 15% phiX spike-in. Library preparation and sequencing were performed at the Genomics and Microbiome Core Facility, Rush University, IL, USA.
identifiers.namespace: SUB15422267
identifiers.id: ExpA_vSM_2