title: RNA-Seq of Larimichthys crocea:  spleen
design: Using fragmented mRNA as a template and random oligonucleotides as primers, the first cDNA chain was synthesized in the M-MuLV reverse transcriptase system, and then the RNA chain was degraded with RNaseH, and the second cDNA chain was synthesized with dNTPs as raw materials in the DNA polymerase I system. The purified double-stranded cDNA was end-repaired, A-tailed, and connected to sequencing adapters. AMPure XP beads were used to screen cDNA of about 250-315bp, PCR amplification was performed, and AMPure XP beads were used to purify the PCR product again to finally obtain the library.
identifiers.namespace: SUB15010410
identifiers.id: PQLYC4_120h-1