title: Ribosome profiling and RNA-seq of Salmonella infected iBMDM
design: Ribosome profiling and RNA-seq of Salmonella infected iBMDM
library_construction_protocol: iBMDM were infected with WT or Î”prgJ Salmonella Typhimurium or mock infected for 60 min. Cells were lysed in buffer containing cycloheximide and chloramphenicol, and lysates were split for RNA-Seq and Ribo-Seq. For Ribo-Seq, lysates were treated with RNase I and fragments protected from digestion by the ribosome were purified. For RNA-Seq, total cellular RNA was fragmented by alkaline hydrolysis. Both produce short RNA fragments, from which libraries were generated. 
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-15534:M RNA_s