title: The 16S rDNA sequence of mice feces B5 (CRX579380; CRA009705)
library_construction_protocol: The genomic DNA was extracted and the integrity of DNA was detected using 1% agarose gel electrophoresis. The V3-V4 region of 16S rDNA gene was amplified and the PCR products were recovered using 1% agarose gel electrophoresis. Then the DNA were purified with Agencourt AMPure XP Nucleic Acid Purification Kit. DNA sequencing was performed based on the Illumina MiSeq platform (PE300). Remove chimeras and short sequences from sequencing data to obtain high-quality sequences and generate operational taxonomic units (OTUs). Finally, OTUs with a similarity level less than 97% were used for further bioinformatics analysis.
identifiers.namespace: Affiliated Hospital of Nanjing University of Chinese Medicine
identifiers.id: DRX708909
identifiers.namespace: NGDC
identifiers.id: CRX579380