title: Illumina metagenome of Med-OCT2021-75m
design: Med-OCT2021-75m-Illumina metagenomic sample was collected at 75 m deep in the epipelagic Mediterranean Sea at 20 nautical miles off the coast of Alicante (Spain) (37.35361N, 0.286194W), in October 2021, when the water column is strongly stratified. 200 L were collected and sequentially filtered on board through 20-, 5-, and 0.22um pore filter polycarbonate filters. Water was directly pumped onto the series of filters to minimize the bottle effect. Filters were immediately frozen on dry ice and stored at -80C until processing. DNA extraction was performed from the 0.22um filter (free-living bacteria) following the MagAttract Purification Kit protocol (QUIAGEN). Metagenome was sequenced using Illumina NextSeq 150 bp paired end reads.
identifiers.namespace: SUB14879673
identifiers.id: Med-OCT2021-75m-Illumina