title: GSM8490617: RNA_seq_mock_rep1; Acanthamoeba castellanii; RNA-Seq
library_construction_protocol: The cell pellets were resuspended with 600 µl lysis buffer (20mM Tris pH 7.5, 150mM NaCl, 5mM MgCl2, 1mM dithiothreitol, 1% Triton X-100, 100 µg/ml of cycloheximide, and 100 µg/ml chloramphenicol), treated with 5 μl of TURBO DNase (2 U/μl, ThermoFisher Scientific) on ice for 10 min, and then centrifuged at 20,000 × g, at 4°C for 10 min. Total RNA was purified using TRIzol-LS and Direct-zol RNA Microprep kit (Zymo Research). RNA-Seq libraries were prepared with TruSeq Stranded Total RNA Library Prep Gold (Illumina).
identifiers.namespace: GEO
identifiers.id: GSM8490617_r1