title: GSM8977467: H3K27ac ChIP-seq, fly adult, day55, replicate 2; Drosophila melanogaster; ChIP-Seq
library_construction_protocol: RNA was isolated using the RNeasy Mini Kit (Qiagen). RNA quality (RIN > 9) was assessed using the TapeStation 4200 system (Agilent Technologies). Approximately 1 μg of RNA was used for sequencing library preparation. Adult fruit flies were homogenized using a Dounce tissue grinder. Cells were fixed with 1% formaldehyde for 10 minutes, followed by quenching and cell lysis. Chromatin was then digested with micrococcal nuclease (MNase) at 37 °C for 15 minutes. Preparation of RNA library and transcriptome sequencing was conducted by Biomarker Technologies (BMK) GmbH (Münster, Germany). Preparation of ChIP library and sequencing was conducted by Biomarker Technologies (BMK) GmbH (Münster, Germany).
identifiers.namespace: GEO
identifiers.id: GSM8977467_r1