title: Lactobacillus iners strain NVM196S05
design: For genome sequencing, 4 mL of Serradors Lactobacilli-modified Iscoves Media was inoculated with a single colony of each L. iners strain. The culture was incubated anaerobically at 37 C for 42 hours. gDNA was then extracted using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, A1120), with the following modifications: 1.5 mL of starting culture was used, and for strains with low DNA yields, two preparations were combined at the re-hydration step. gDNA libraries were prepared using the Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24). Following DNA extraction and library preparation, gDNA was subjected to long-read DNA sequencing on a MinION Mk1B using Kit 14 chemistry (R10.4.1). Basecalling was perfomed using Dorado (v0.8.3, using the SUP basecalling model).
identifiers.namespace: SUB15441194
identifiers.id: 196-05