title: GSM8804977: p2_IP_VDJ; Homo sapiens; OTHER
library_construction_protocol: PB nad BM-derived cells  were incubated with scFv-BT (Jackson Immunoresearch) for CAR detection, washed with PBS/BSA and stained with Streptavidine-BV421 (BD Biosciences). CAR T cells were then sorted either with BD FACSAria IIu or Moflo ASTRIOS BC. FACS-sorted CAR T cells from BM and PB samples of MM patients were subjected to scRNA-seq using the Chromium Single Cell 5′ Reagent Kit (10X Genomics) according to manufacturer's instructions. TCRα/β sequencing was performed with 10X Genomics Single Cell V(D)J Immune Profiling Solution (10x Genomics).
identifiers.namespace: GEO
identifiers.id: GSM8804977_r1