title: RNA-seq of Arabidopsis thaliana mir394a mir394b 14 days-old
design: For transcriptome experiments, aerial part of plants was sampled 14 d after germination, and total RNA was extracted from three biological replicates, being each biological replicate a pool of three plants. Total RNA was isolated from 100 mg of pooled plants using TriPure reagent (Roche), followed by RQ1 DNase (Promega) treatment and posterior purification using the PureLinkTM RNA mini kit (Invitrogen). Poly(A) RNA-Seq sequencing was performed by LC Science (Houston, TX, USA) on strand-specific cDNA libraries, sequenced as 150-bp paired-end reads using Illumina NovaSeq.
identifiers.namespace: SUB14805015
identifiers.id: DM3