title: GSM8806408: RNA-seq of CRISPR Y-KO MB49 cells Replicate 3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen) with gDNA Eliminator columns, following the manufacturer's protocol. RNA-seq libraries were prepared using the Illumina TruSeq Stranded mRNA Library Prep Kit. Poly-A RNA was enriched, fragmented, and reverse-transcribed into cDNA, followed by PCR amplification.
identifiers.namespace: GEO
identifiers.id: GSM8806408_r1