title: Illumina HiSeq 2500 sequencing
library_construction_protocol: DNA libraries of Halomonas sp. MARW-1-7-2 were prepared using a Nextera XT Library Prep Kit (Illumina, San Diego, USA) as per the manufacturer's protocol with the following modifications: an input of two nanograms of DNA was used instead of one nanogram, and the PCR elongation time was increased from 0.5 to 1 min. DNA quantification and library preparation were carried out on a Hamilton Microlab STAR automated liquid handling system. Pooled libraries were quantified using a Kapa Biosystems library quantification kit for Illumina platform on a Roche light cycler 96 qPCR instrument. Libraries were sequenced on a short-read sequencing Illumina HiSeq platform using a 250 bp paired end protocol.
identifiers.namespace: edge hill university
identifiers.id: ena-EXPERIMENT-TAB-30-11-2023-15:34:40:700-16990