title: snRNA-seq of adult human macular region of retina
design: Samples included in this study were retinal tissues of donors from the Utah Lions Eye Bank collected within 6 hours post-mortem. All donors were screened for medical history, and only the ones with no retinal diseases were used in this study. Post-mortem phenotyping with OCT were performed to confirm that there were no drusen, atrophy, or any other disease phenotypes on retina. With 4mm and 6mm disposable biopsy punches, macula, fovea, and peripheral lobe of retina were collected and flash frozen in liquid nitrogen, and stored at -80C before nuclei isolation. Nuclei for snRNA-seq were isolated by fresh-made pre-chilled RNase-free lysis buffer. The frozen tissue was resuspended and triturated in lysis buffer and homogenized with a WheatonTM Dounce Tissue Grinder. NeuN samples were enriched for neuron nuclei by FACS. The isolated nuclei were stained with NeuN and sorted with a BD Aria II flow sorter (70m nozzle).  Single nuclei Gene Expression libraries were prepared according to Chromium Next GEM Single Cell 500 3' Reagent Kits v3.1 (10x Genomics). Single nuclei ATAC libraries were prepared according to Chromium Next GEM Single cell ATAC 511 Reagent kit v1.1 (10x Genomics). Multiome GEX and ATAC libraries were prepared according to Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Kits v1 (10x Genomics).
identifiers.namespace: SUB14807216
identifiers.id: MMD_22_18633_RNA_macular_lib