title: Hybrid capture: Cargo-only-GT-rep2
design: Genomic DNA extracted from edited K562attB cells were used as input for hybridization capture NGS. A Covaris ME220 focused ultrasonicator was used to shear 400 ng of Rep 1, Rep 2, Control 1, and Control 2 gDNA samples to an average size of 400 bp. Fragmented DNA was end repaired and A-tailed followed by ligation of Illumina sequencing adapters using Twist library preparation kit (cat#104177) to create Illumina paired end sequencing libraries. Hybridization, capture and post capture amplification of the prepared libraries to probes was performed using Twist target enrichment standard hybridization kit as per manufacturers instructions (Twist Library Preparation Kit 1, Mechanical Fragmentation, cat # 100876). Libraries were sequenced on Illumina NextSeq 2000 platform using a P2 600 cycle kit (Illumina) according to standard manufacturers protocol.
identifiers.namespace: SUB14774691
identifiers.id: Cargo-only-GT-rep2