title: Denovo WGS seqeuncing of bacteria Styphylococcus aureus
design: Whole genome sequencing (WGS) library preparation for bacterial samples begins with extraction of high-quality genomic DNA from the cultured isolate, followed by assessment of purity and concentration. The genomic DNA is then fragmented to an average size of approximately 300500 bp using enzymatic shearing. The fragmented DNA undergoes end repair to generate blunt ends and A-tailing to add a single adenine overhang at the 3 ends. Sequencing adapters are subsequently ligated to both ends of the DNA fragments. The adapter-ligated products are purified and size-selected using magnetic beads to enrich fragments of the desired size range. The resulting library is then amplified using limited PCR cycles. The final library is quantified and checked for fragment size distribution using tape station. Once validated, the library is diluted to the required concentration and loaded onto the NovaSeq 6000 sequencing platform.
identifiers.namespace: SUB15521960
identifiers.id: WGS_Lib_001