title: Sequences of gene
design: Total RNA was extracted using TRIzol Reagent (Invitrogen) and genomic DNA was removed using DNase I. mRNA was isolated with polyA selection by oligo(dT) beads and fragmented using fragmentation buffer. cDNA synthesis, end repair, A-base addition and ligation of the Illumina-indexed adaptors were performed following TruSeq RNA sample preparation Kit protocol. Libraries were size selected for 200-300 bp cDNA target fragments on 2% Low Range Ultra Agarose and PCR amplified for 15 cycles. The libraries were sequenced on the Illumina HiSeq platform with 2x150bp paired-end reads.
identifiers.namespace: SUB15994362
identifiers.id: 12LG2G_R1