title: GSM8771278: IR64, second latest leaf, rehydration after severe drought, Day 0 (2h), rep 3; Oryza sativa; RNA-Seq
library_construction_protocol: The fully expanded latest leaves and second latest leaves were collected and frozen in liquid nitrogen. Samples were ground to a fine powder with a multi-beads shocker and used for RNA extraction. Total RNA was extracted using guanidine isothiocyanate based buffer and magnetic beads as described previously (Yoshino et al., 2020). RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (E7760L) according to the manufacture's protocol.
identifiers.namespace: GEO
identifiers.id: GSM8771278_r1