title: WGS by CD Genomics using Illumina
design: DNA extraction by tissues grinding in mortar and pestle with liquid nitrogen, proteinase K and RNA digestions; deproteinization with 3 M potassium acetate and 11.5% (v/v) glacial acid acetic solution, and precipitation by isopropanol and 70% ethanol. DNA resuspension in ultrapure water. Insert size 350 bp. NEBNext Ultra II DNA Library. PE150 bp. Sequencing 2 samples per flow cell.
identifiers.namespace: SUB14849336
identifiers.id: NATRufi205_279