title: Ribo-seq of Eif6 WT, Eif6 S235A, Eif6 S235A rescued  with eIF6WT and high salt treated MEFs
design: Ribo-seq of Eif6 WT, Eif6 S235A, Eif6 S235A rescued  with eIF6WT and high salt treated MEFs
library_construction_protocol: Immortalized MEF cells were washed once with ice-cold PBS 10ug/ml cycloheximide. digestion without KCl treatment Cells were lysed in 20mM Tris-HCl, 50mM NaCl, 10mM MgCl2, 1mM DTT, 100ug/ml cycloheximide, 1% NP-40, 0.2mg/ml heparin. Ribosome Protected Fragments (RPF) were obtained following Ingolia 2017 (10.1016/j.ymeth.2017.05.028).  Immagina Biotechnology LACEseq #LS-001_12
identifiers.namespace: European Bioinformatics Institute
identifiers.id: E-MTAB-14864:Sample 7_s