title: GSM8878832: pulmonary ILCs_Afu_17h_1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from frozen samples using Quiagen RNAeasy Plus Micro 74035 Kit The double-strand cDNA was synthesized by LD-PCR amplification (SMART-Seq® v4 Ultra® Low Input RNA Kit), followed by purification with AMPure XP beads and quantification with Qubit. The cDNA samples were then fragmented, end-repaired, A-tailed, and ligated with Illumina adaptors. After size selection and PCR-enrichment, RNA libraries were checked with Qubit and real-time PCR for quantification, and 5400 Bioanalyzer System (Agilent Technologies) for size distribution.
identifiers.namespace: GEO
identifiers.id: GSM8878832_r1