title: CFTR-/- CD4.2
design: RNA was isolated using a RNeasy Micro Kit (Qiagen) following manufacturer's instructions and  Illumina Ribo-Zero Plus rRNA Depletion kit was used to isolate rRNA-depleted RNA. The samples were then reverse transcribed to create cDNA. The cDNA was fragmented, blunt-ended, and ligated to indexed adaptors. Following quantification of the cDNA generated for the library, the samples were clustered and loaded equally over two lanes on an Illumina NovaSeq6000 Sequencing system (Illumina Inc.).
identifiers.namespace: SUB14775040
identifiers.id: CFTR-/- CD4.2