title: Shotgun metagenomics of tick:Amblyomma variegatum
design: Genomic DNA was extracted from whole ticks using the Qiagen DNeasy Blood & Tissue Kit (Qiagen, USA) following the manufacturers protocol optimized for arthropod tissues. The experimental procedures were performed according to the standard protocol provided by Illumina, including sample quality assessment, library preparation, library quality control and sequencing.DNA passing quality assessment was fragmented. DNA fragments were further processed for end repairing, adding 3' A tail and adapter ligation. The products of proper size were selected and purified. The selected fragments were further amplified to construct sequencing library. Qualified libraries were then processed for sequencing on Illumina platform.
identifiers.namespace: SUB15307639
identifiers.id: av4