title: GSM8905032: Male_A; Rattus norvegicus; RNA-Seq
library_construction_protocol: On P4, neonates of both sexes (n=6) were deeply anesthetized with Fatal Plus and decapitated. Brains were removed and placed on ice, and the amygdala of both hemispheres was dissected out and placed in Hibernate A solution (Thermo Fisher Scientific, Waltham, MA) while the remaining brains were dissected. Single cells were isolated for transcriptional profiling by proteolytic enzymatic digestion using the Worthington Papain Dissociation System (Worthington Biochemical Corporation, Lakewood, NJ), following the manufacturer's protocol. Samples were centrifuged, then resuspended in 0.05% bovine serum albumin in phosphate-buffered saline. Cell suspensions were then pooled, resulting in four final samples prepared for submission: 2 male samples and 2 female samples, each containing cells from 3 animals per sex. Pooled suspensions were counted using a hemocytometer and delivered for further processing using the 10x Genomics system. A total of 3,000 cells per sample were loaded by GRC staff into each well of a chromium microfluidics container (10x Genomics, Pleasanton, CA). Sequencing libraries were prepared using the Chromium Next GEM Single Cell 3' Kit v2 per the manufacturer's instructions. Samples were sequenced across 4 lanes (1 sample per channel) using the 75 base pair paired-end protocol on an Illumina HiSeq4000 sequencer.
identifiers.namespace: GEO
identifiers.id: GSM8905032_r1