title: LDHB KO_3_RNA (CRX1181252; CRA018798)
library_construction_protocol: Cultured LDHB KO CD8+ T cells under in vitro exhaustion conditions for 8 days were chosen for ATAC-seq analysis. The ATAC-seq libraries were meticulously prepared using the TruePrep DNA Library Prep Kit V2 and TruePrep Index Kit V2 for Illumina (Vazyme), following the manufacturer's guidelines. Post-preparation, the libraries underwent a purification process utilizing VAHTS DNA Clean Beads. Library quality was assessed with the Agilent 2100 Bioanalyzer system. Sequencing was performed on the Illumina NovaSeq platform, targeting 60 million reads for each sample. Following sequencing, Fastq files were processed using fastp and then aligned with the mm10 reference genome via hisat2(v2.2.0). Individual peaks were identified using macs2(v2.1.2) and subsequently filtered to retain those consistently present across multiple replicates.
identifiers.namespace: NGDC
identifiers.id: CRX1181252
identifiers.namespace: Shanghai Jiaotong University School of Medicine
identifiers.id: DRX718710