title: GSM9187624: Lib7_SkinWound; human skin metagenome; OTHER
library_construction_protocol: ZymoBIOMICS® DNA Miniprep Kit (Zymo Research, Irvine, CA) was used for low biomass samples such as skin swabs, as it permits for a lower elution volume, resulting in more concentrated DNA samples. Bacterial 16S ribosomal RNA gene targeted sequencing was performed using the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). The bacterial 16S primers amplified the V1-V3 region of the 16S rRNA gene. These primers have been custom-designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity.  The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity. The final pooled library was cleaned with the Select-a-Size DNA Clean & Concentrator™ (Zymo Research, Irvine, CA), then quantified with TapeStation®(Agilent Technologies, Santa Clara, CA) and Qubit® (Thermo Fisher Scientific, Waltham, WA).
identifiers.namespace: GEO
identifiers.id: GSM9187624_r1