title: GSM8663115: sample1; Gallus gallus; RNA-Seq
library_construction_protocol: Chicken embryo somites were electroporated at E2.5 at the level of the forelimb with a plasmid coding for a constitutive nuclear dTomato and a mVenus under the control of a TCF/LEF-responding promoter. Embryos were screened for good electroporation 2 days later (E4.5). Nicely electroporated limb bud were dissected and digested with Dispase and Trypsin accoding to Ling et al. 2019, Nat. Cell Biol.). The single cell preparation was then shorted for the dTomato fluorescence and dTomato+ cells were submitted to 10x genomics single RNA-seq Libraries were prepared according to 10x 3'expression V2 kit  & Illumina protocols
identifiers.namespace: GEO
identifiers.id: GSM8663115_r1