title: GSM8976610: S7_DOC_LAV; Mus musculus; RNA-Seq
library_construction_protocol: After two weeks, germinal center B cells were isolated from recipient mice by FACS sorting of live, CD45.1+ GFP+B220+ germinal center KL25 B cells in TAKARA lysis buffer, following the vendor's instructions, and frozen at -80°C until sequencing. RNA-seq library preparation was performed at the Genomics Facility Basel using a SMART-Seq stranded kit (Takara), and controlled on an Agilent Fragment Analyzer instrument. Sequencing was performed on four lanes of an Illumina NextSeq 500 instrument to produce paired-end 38nt reads.
identifiers.namespace: GEO
identifiers.id: GSM8976610_r1