title: GSM8584195: Mouse Embryoid body (day 2) from Epiblast stem cells; Mus musculus; RNA-Seq
library_construction_protocol: Mouse epiblast cells and embryoid bodies were collected at the specified stages and single cells were dissociated using Trypsin treatment and counted to load 10,000 cells into on channel of a 10X Chromium chip.  One channel per sample was used. After cell separation and reverse transcription reactions occurred, library preparation followed in accordance with the Chromium Next GEM Single Cell 3' v3.1 (Dual Index) protocol.  Libraries were sent to Novogene for sequencing. Single cell suspensions were passed through 40μm cell strainer (Corning) and concentration was adjusted to 1000 cells/μL. Suspensions were loaded in single-cell-G Chip (10X Genomics) for target output of 10,000 cells per sample. Single-cell droplet capture was performed on the Chromium Controller (10X Genomics). cDNA library preparation was performed in accordance with the Single-Cell 3' v 3.0 or v3.1 protocol. Libraries were evaluated for fragment size and concentration using Agilent HSD5000 ScreenTape System (Agilent). Samples were sequenced using paired-ended, dual indexing, using cycles 28, 10, 10, 90 (R1, i7, i5, R2), for an output of 200 million reads per sample.
identifiers.namespace: GEO
identifiers.id: GSM8584195_r1