title: Syn1.0 PacBio Replicate 3
design: 20ng of ribodepleted and poly-A tailed RNA were converted to cDNA with the Iso-Seq Express Oligo Kit. cDNAs were barcoded with the Barcoded Overhang Adaptor Kit 8A, and converted into a library with the SMRTBell Prep kit 3.0 (Pacific Biosciences). The libraries were pooled in equimolar concentration and the pool was sequenced on 1 SMRTcell 8M on a PacBio Sequel IIe using the CCS sequencing mode and a 30hs movie time.
identifiers.namespace: SUB14940117
identifiers.id: 0003