title: GSM8975911: IR_rep2; Homo sapiens; RNA-Seq
library_construction_protocol: After 7 days of culture, total RNA was extracted from all groups (n=3 per group) using the RNeasy Plus Mini Kit (Qiagen) following the manufacturer's instructions. RNA concentration was measured using the Nanodrop ND-1000, and quality was assessed via capillary electrophoresis (Agilent Technologies). RNA sequencing (RNA-Seq) was performed by Novogene Corporation Inc. Libraries were prepared using the KAPA Stranded RNA-Seq Library Prep Kit (Illumina), and sequencing was conducted on the NovaSeq 6000 platform (Illumina) to generate paired-end reads. Total RNA from all groups were sent to Novogene Corporation Inc. (Sacramento, CA) for library preparation and sequencing. Only samples with an RNA integrity number (RIN) ≥ 7 were considered for cDNA library preparation. cDNA libraries were generated using TruSeq Stranded Total RNA Gold Sample Prep Kits, (Illumina), which included an rRNA suppression step.
identifiers.namespace: GEO
identifiers.id: GSM8975911_r1