title: RNA-Seq of gut microbiota: health control
design: The characteristics of intestinal microbiota were analyzed by 16S rRNA amplicon sequencing of fecal samples. Total DNA was extracted from fecal samples using the cetyltrimethylammonium bromide method. PCR amplification of the V3-V4 region of the 16S rRNA gene was performed using primers 341F (5-CCTAYGGGRBGCASCAG-3) and 806R (5-GGACTACNNGGGTATCTAAT-3).
identifiers.namespace: SUB14774248
identifiers.id: HC_5