title: GSM8978717: Hu-P-3828; Homo sapiens; RNA-Seq
library_construction_protocol: RNA were extracted from four 10µm sections from each FFPE block using the Qiagen AllPrep FFPE Kit (Cat no. 80234; Qiagen, Germantown, MD, USA) following the manufacturer's protocol For RNA-seq, RNA concentration and integrity were assessed using the Qubit fluorometer with the RNA High Sensitivity Assay Kit (Cat no. Q32852; ThermoFisher Scientific, Waltham, MA, USA) and using the Fragment Analyzer with Agilent High Sensitivity RNA Kit (Cat no. DNF-472; Agilent Technologies, Santa Clara, CA, USA). RNA was used as an input material for library construction using Illumina RNA Prep with Enrichment (L) Tagmentation Kit with the Illumina Exome Panel (Cat no. 20040537 and Cat no. 20020183; Illumina, San Diego, CA, USA) following the manufacturer's protocol. The libraries were multiplexed and then sequenced on either the Illumina NextSeq2000 or NovaSeq6000 (Illumina, San Diego, CA, USA), generating 2x100bp-paired end reads with a mean aligned pair-read count of 28 Million reads per sample.