title: Single cell sequencing of PBMC from childhood ITP patients with transient or chronic disease course
design: Single cell sequencing of PBMC from childhood ITP patients with transient or chronic disease course
library_construction_protocol: Whole blood collected by venipuncture. PBMC isolated by ficoll and cryopreserved. PBMC samples were thawed in RPMI and 10% FCS and concentration was adjusted according to 10x genomics instructions. Cells were loaded in 5' Chromium Next GEM Single Cell Immune Profiling kits for gene expression v3.1. Human T and B cell Chromium Single Cell V(D)J Enrichment kits were used for targeted TCR and Ig enrichment. Steps were performed according to the manufacturer protocol.  Steps were performed according to the 10X manufacturer protocol.