title: Targeted sequencing of aldosterone-driver genes in human adult adrenals from unilateral primary aldosteronism patients
design: The qualified genomic DNA sample was randomly fragmented by Covaris technologyand the size of the library fragments was mainly distributed between 150bp and 250bp.The end repair of DNA fragments was performed and an "A" base was added at the 3'-end of each strand. Adapters were then ligated to both ends of the end repaired/dA tailedDNA fragments for amplification and sequencing. Size-selected DNA fragments wereamplified by ligation-mediated PCR (LM- PCR ), purified, and hybridized to the exomearray for enrichment. Non-hybridized fragments were then washed out. Capturedproducts were then circularized. The rolling circle amplification (RCA) was performed toproduce DNA Nanoballs (DNBs). Each resulting qualified captured library was thenloaded on DNBSEQ sequencing platforms