title: RNA-seq of mix stage with drought stress of Aphelenchus avenae
design: After the mixed-age nematodes were separated from the culture dish, sucrose was used for centrifugation to remove impurities, and then the nematodes were placed in a completely dry environment for ten minutes to dehydrate. Then they were transferred to PBS buffer for recovery and Trizol was quickly added and quick-frozen. RNA was then extracted and RNA-seq library was constructed and sequenced.