title: GSM9246427: LCAC2; Rattus; RNA-Seq
library_construction_protocol: Adult rat cardiomyocytes (ACMs) were serum-starved overnight and divided into four groups. They were then treated with palmitoyl-L-carnitine (LCAC C16) (P1645, Sigma-Aldrich), at concentrations of 0150 μM, respectively, for 4 hours. Messenger RNA was purified from total RNA using poly-T oligo-attached  magnetic beads. After fragmentation, the first strand cDNA was  synthesized using random hexamer primers followed by the second  strand cDNA synthesis. The library was ready after end repair, A-tailing,  adapter ligation, size selection, amplification, and purification. The  library was checked with Qubit and real-time PCR for quantification and  bioanalyzer for size distribution detection