title: GSM9284661: T. cruzi epimastigotes Control T7/Cas9 - replicate 2; Trypanosoma cruzi; RNA-Seq
library_construction_protocol: Total RNA from each sample analyzed were isolated using the RNeasy Mini Kit (Qiagen) and treated with RNAse-free DNAse I (Qiagen). RNA samples were quantified and evaluated for integrity using the fluorometer Qubit 4 (Thermo Scientific). All samples showed an A260/A280 ratio close do 2.0. Poly-A + RNA was selected by oligo-dT chromatography and used to generate cDNA libraries for massive sequencing using the TruSeq RNA Sample Prep Kit (Illumina) following manufacturer's protocols.