title: GSM8703904: CD133+PD-L1+, rep2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using RNeasy Micro kit (Qiagen) or RNAmini kit (Qiagen) according to manufacturer's instructions. For the sorted CD133+ and CD133- B16F10 cancer cells, libraries were constructed using the TruSeq RNA sample preparation kit (Illumina), and sequencing was performed on Illumina Novaseq 6000 instrument with 150 bp paired-end reads by Genergy BIO-technology (Shanghai, China). For the other samples, the library construction and sequencing were performed by Beijing Genomics Institute (BGI, Shenzhen, China) using BGISEQ-500 platform. Single end 50 bases reads were used for the cultured macrophages or the tumor tissue samples, and pair end 100 bases reads for sorted GFP+CD133+PD-L1+ and GFP+CD133-PD-L1- cancer cells from the relapsed B16F10 tumors.