title: GSM9363175: leaf,RNA-seq,WT-FL-1; Solanum tuberosum; RNA-Seq
library_construction_protocol: Leaf samples were collected with three biological replicates per treatment. Total RNA was extracted from 100 mg of fresh tissue using TRIzol reagent (TransGen Biotech, Beijing, China). RNA integrity was assessed using an Agilent 2100 Bioanalyzer, and only samples with RNA integrity numbers (RINs) > 8.0 were used for library construction. RNA-seq libraries were prepared using 2-5 μg of total RNA, and the constructed libraries had fragment sizes ranging from 250 to 300 bp. Sequencing was performed on the Illumina HiSeq 2000 platform, with a minimum sequencing depth of 6 Gb per sample and paired-end sequencing of 150 bp read length. Leaf samples were collected with three biological replicates per treatment. Total RNA was extracted from 100 mg of fresh tissue using TRIzol reagent (TransGen Biotech, Beijing, China). RNA integrity was assessed using an Agilent 2100 Bioanalyzer, and only samples with RNA integrity numbers (RINs) > 8.0 were used for library construction. RNA-seq libraries were prepared using 2-5 μg of total RNA, and the constructed libraries had fragment sizes ranging from 250 to 300 bp. Sequencing was performed on the Illumina HiSeq 2000 platform, with a minimum sequencing depth of 6 Gb per sample and paired-end sequencing of 150 bp read length.