title: 16S rRNA Gut metagenome of Chelonia mydas:Guanabara Bay
design: DNA was extracted from cloacal samples using a ZymoBIOMICS DNA Miniprep Kit (Zymo Research, Irvine, CA, USA), according to the manufacturers instructions. The V6V7-V8 highly variable portion of the bacterial 16S rRNA gene (forward - CGA WGCAACGCGAARAACCT, reverse - CGAGTTGCAGKSWVCYATCCG) (modified from DIAS et al., 2020) was amplified. The reactions were carried out in the Veriti-96 well Thermal Cycler with the following programming: activation of the enzyme for 3 min at 94C, followed by 40 cycles of 94C for 30 s, 58C for 30 s, 68C for 1 min, concluding at 4C. The amplified sequences were quantified using a Qubit fluorometer 4 (Thermo Fisher Scientific, United States). Finally, the quantified libraries were mixed equimolarly to generate the equimolar pool. Clonal amplification and library enrichment were carried out on the Ion Chef (Thermo Fisher Scientific, United States). The purified amplicons were pooled in equimolar quantities and sequenced using an Ion GeneStudio S5 System platform (Thermo Fisher Scientific, United States).