title: GSM8613124: mouse liver, D5, ATAC, Multiome; Mus musculus; ATAC-seq
library_construction_protocol: Combined scRNA- and scATAC-seq (10x Multiome) was performed as described in the demonstrated protocol for nuclei isolation for single cell Multiome ATAC + Gene Expression Sequencing (CG000365.Rev C). For this, liver cell suspensions were FACS-sorted for alive, Cd45+, Cd64- Cd11c+ cells. Sorted cells were then used for nuclei extraction. Digitonin treatment was performed for 210 s, nuclei suspensions washed, quantified and loaded at a concentration between 3000 – 8000 nuclei/µl to target a recovery of 10.000 nuclei. 10x Multiome preparation according to demonstrated protocol CG000365.Rev C Combined scRNA- and scATAC-seq (10x Multiome), Single Cell Multiome ATAC + Gene Expression v1