title: Shotgun metagenomics sequencing of sample Kidman_130
design: Libraries were indexed using IDT for Illumina Nextera DNA Unique Dual Indexes (Illumina, Australia) and purified using an 18% suspension of Sera-Mag Speed-beads Carboxyl Magnetic Beads (GE Healthcare), added in a ratio of 1.8:1 vol PCR product. Libraries were quality checked using gel electrophoresis, quantified using Qubit (Invitrogen) according to the manufacturers instructions sequenced on an Illumina Novaseq 6000 using paired-end 150 bp reads at the Australian Centre for Ecogenomics, The University of Queensland.