title: RNA-Seq of tambaqui subjected to F. oreochromis infection: Asymptomatic
design: After infection with F. oreochromis, skin samples were collected from asymptomatic individuals, and 5 libraries were formed. RNA was extracted using the TRIzol reagent (Invitrogen). Total RNA quantification was performed using the Qubit RNA kit on a Qubit 2.0 Fluorometer (Life Technologies). Integrity was verified by 1.5% gel electrophoresis, identifying the 28S and 18S bands, respectively. Sample purity was assessed using the NanoDrop One (ThermoFisher).