title: Prostate cancer associated fibroblasts have distinct morpho-mechanical features that are associated with patient outcome
design: All RNA samples were diluted into the appropriate concentration range (based upon NanoDrop measurements provided) and then QC'd by Bioanalyzer for quality and Qubit for quantity. Samples were processed each starting with 20ng of total RNA. Libraries were made by individual first strand synthesis to add the index sequences indicated above. Following first strand synthesis cDNA of each set was pooled for subsequent processing. The library pools were QC'd by Qubit, Bioanalyzer and qPCR. They had a mean size of ~430bp at a concentration of ~4nM. Samples were sequenced using Illumina NextSeq550 High output mode and v2.5 chemistry.