title: RNA-seq shows genes enhanced in translation and transcription pathways in MKs and platelets from Slfn14fl/flPF4-Cre mouse model
design: RNA-seq shows genes enhanced in translation and transcription pathways in MKs and platelets from Slfn14fl/flPF4-Cre mouse model
library_construction_protocol: Mice were exsanguinated under terminal anesthesia by isoflurane/O2 (5%) gas. Blood was drawn from the inferior vena cava, using a 25-gauge needle, into 1:10 (volume-to-volume ratio) acid citrate dextrose anticoagulant.  Platelets and megakaryocytes were isolated and RNA was extracted using the RNeasy mini kit (Qiagen) according to manufacturer's instructions Illumina/NEB kit protocol - Libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina with NEBNext® rRNA Depletion Kit v2 (Human/Mouse/Rat) according to the manufacturer's protocol. During this process, the libraires were indexed using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs) Set 4. The prepared libraries were quantified via a fluorometric method involving a Promega QuantiFluor dsDNA assay and qualified using electrophoretic separation on the Agilent TapeStation 4200.