title: GSM8910745: RH-3.IP; Toxoplasma gondii; OTHER
library_construction_protocol: Input: Total RNA was extracted from tachyzoites with TRIzol reagent (Invitrogen Carlsbad, CA, USA). IP:Total RNA was extracted from tachyzoites with TRIzol reagent (Invitrogen Carlsbad, CA, USA), and then subjected to immunoprecipitation (IP) by using the GenSeq m5C-IP Kit (Cloudseq Biotech, Inc., Shanghai, China) following the manufacturer's specifications. RNA libraries for IP and input samples were constructed with the GenSeq® Low Input Whole RNA Library Prep Kit (Cloudseq Biotech, Inc., Shanghai, China) according to the manufacture's instructions.