title: GSM8704774: GV (MS238_132); Mus musculus; RNA-Seq
library_construction_protocol: For the SC3-seq samples, dissociated oocytes and granulosa cells from in vivo mouse ovarian follicles and in vitro oocyte-like cells were manually picked up using glass capillaries under a stereomicroscope (Leica, S8 APO).  The subsequent cDNA synthesis and amplification methods were as previously described (Kurimoto et al, 2006, 2007; Nakamura et al, 2015, 2016). and the 3' termini of cDNA were subjected to sequencing on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina). For the SC3-seq samples, the subsequent cDNA synthesis and amplification methods were as previously described (Kurimoto et al, 2006, 2007; Nakamura et al, 2015, 2016). and the 3' termini of cDNA were subjected to sequencing on the Illumina NextSeq 500/550 platform with a NextSeq 500/550 high output kit v. 2 (75 cycles) (FC-404-2005; Illumina).