title: GSM8979543: CFU130 Nasal cells HIV infected, ART greater than 1 year; Homo sapiens; RNA-Seq
library_construction_protocol: Nasal cells collected using nasal currette were dislodged by pipetting liquid up and down the tip of the curette, and cells were centrifuged at 500g for 10 min at 4 °C. The supernatant was completely removed, and cells were resuspended in 500 µl of PBS (Fisher Scientific) counted snd loaded onto a chromium controller. Single-cell RNA libraries were prepared using the Chromium Next GEM Single Cell 5′ Reagent Kit v2 and Chromium Controller (10× Genomics), following the manufacturer's protocol. We loaded 10,000-16,000 nasal single-cell suspensions, with a minimum viability greater than 80%, into microfluidic chips to generate Gel Beads-in-Emulsion (GEMs), where individual cells were lysed, and mRNA barcoded using oligo(dT) primers with unique molecular identifiers (UMIs) and cell barcodes. Template switching was facilitated by a template switch oligonucleotide (TSO) to enable full-length cDNA synthesis. GEMs were broken, and barcoded cDNA was recovered and amplified by PCR.