title: RNA-seq of mix stage with freez stress of Aphelenchus avenae
design: After the mixed-age nematodes were separated from the culture dish, impurities were removed by sucrose centrifugation, and the nematodes were then cultured in a 4C refrigerator overnight, and then Trizol was quickly added and snap-frozen. RNA was then extracted and an RNA-seq library was constructed and sequenced.