title: TAS of human: blood
design: All PCR reactions were pooled at equal quantities with no pool having two overlapping amplicons. These pools were then processed and barcoded using the KAPA HyperPrep kit (KK8501-KK8505, Roche) and UDIs for Illumina Platforms (ILM 20023784, IDT) according to the manufacturers instructions. Processed pools were combined to achieve an equal representation of each amplicon at an overall final concentration of 10nM in 30L for submission to the Genomics and Microarray Sequencing Core at CU Anschutz (Genomics Shared Resource RRID: SCR_021984). Sequencing was performed on an Illumina NovaSEQ X for paired-end sequencing (2x150bp) at a minimum read depth of 50M reads per sample.