title: GSM9269480: PFMP_Batch2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from SVZ neural progenitors (NP) using NucleoSpin® RNA XS according to the manufacturer's instructions. PolyA plus RNAs were amplified and enriched using Ambion's messageAmp II aRNA amplification kit. The quality of amplified aRNA was verified using the Bioanalyzer. A cDNA library was constructed using the Illumina TrueSeq protocol. The barcoded cDNA library was purified with AmpureXP beads and quantified using Qubit High sensitivity DNA kit and the quality of the library was determined by analyzing on Agilent bioanalyzer. Libraries mixed at equimolar ratios were sequenced on a NextSeq500 (Illumina, San Diego, CA) for 75 cycles.