title: GSM8841661: HET6_SAN_HCN4pos; Mus musculus; OTHER
library_construction_protocol: Formalin-fixed paraffin embedded E17.5 fetusses were sectioned at 5 µm. The samples were prepared according to the GeoMx Manual Slide Preparation User Manual, MAN-10150-3 using rabbit-anti-HCN4 (Merck Millipore AB5805), goat-anti-cTnI (Hytest 4T21/2) and Sytox Green Nucleic Acid Stain (Thermo Fisher Scientific S7020) antibodies and the GeoMx Whole Transcriptome Atlas Mouse RNA Probes for NGS (Bruker Spatial Biology). Spatial transcriptome sequence libraries were constructed and sequencing using the NextSeq2000 (P2.100).