title: Biofilm ITS1-2 amplicon sample Event 2 Site D After rep 2
design: DNA was extracted from biofilm sample using the DNeasy PowerSoil kit (Qiagen). DNA concentration and purity were measured using a NanoDrop 2000 (ThermoScientific). Amplification and sequencing of the biofilm DNA samples were completed by the sequencing provider MrDNA (Shallowater, Texas, USA) on an Illumina Miseq. Returned sequence data were demultiplexed using cutadapt (Martin, 2011) with one allowed error. Provided demulitplexed sequences are untrimmed (contain barcode and forward and reverse primer).