title: GSM8703642: FACS-sorted neutrophils from blood of mice with brain tumors (SB28), sample 2; Mus musculus; RNA-Seq
library_construction_protocol: Mice were injected with 10,000 SB28. After 18 days, neutrophils were sorted from tumor and blood. Blood was collected by submandibular puncture followed by cardiac puncture (right atrium). After blood collection, mice were perfused with 20 mL of PBS 1X through the left ventricle and the tumor was collected and process as previously described. Neu were then FACS sorted in MACS buffer, as previously described. After sorting, Neu were washed in RPMI containing 10% FBS and 1U/μL of Protector RNase inhibitor (Sigma, PN-3335399001). The media was filtered through 0,22 μm before the use. Cells were then counted and resuspended at a concentration of 1,000 cells/μL. 20,000 cells per lane were loaded for encapsulation. Cells were then processed with 10X Chromium 3' scRNA-seq, 40,000 reads/cell using NovaSeq S1-100 and NovaSeq SP-100 sequencing run