title: GSM9013247: isoxyl, rep 2; Mycobacterium tuberculosis; RNA-Seq
library_construction_protocol: Bacteria were isolated by centrifugation and resuspended in Trizol LS (Ambion). Samples were lysed by bead beating and the RNA was isolated by chloroform extraction, isopropanol precipitation, and resuspended in nuclease-free water. Sequencing was performed by SeqCenter (Pittsburgh, PA) after samples were treated with Invitrogen DNAse (RNAse free). Ribosomal RNA was depleted using custom Mtb specific oligonucleotides and library preparation was performed using Illumina's Stranded Total RNA Prep Ligation and 10bp unique dual indices (UDI).