title: 16S amplicon sequencing of long-chain alkane-degrading consortium
design: Amplicon metagenomic sequencing was conducted to analyze the microbial population's composition and abundance. DNA extraction from the cell pellet of the consortium was performed following Basim et al.'s (2020) method. PCR reactions were carried out using the [515F, 926R] primer set to create V4-V5 of 16S rRNA library, which was sequenced on the Miseq Illumina platform.