title: GSM8705096: D12 inner ear organoid culture, otic vesicle, TGFβ inhibition on D3, BSFL rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Isolated otic vesicles from mouse embryos (2-6 pooled per sample) and organoid cultures (30-50 pooled per sample) were placed in RLT Buffer (Qiagen) and stored at -80°C until total RNA was extracted using RNeasy Mini Kits (Qiagen). RNA quality was evaluated on Agilent 2200 TapeStation before library construction. RNA libraries for RNASeq were prepared using TruSeq LT RNA Sample Prep Kit 2 (Illumina).