title: GSM8705224: E14.5 cKO scRNA-seq; Mus musculus; RNA-Seq
library_construction_protocol: LACE-seq: RNA immunoprecipitation (IP) was performed using anti‐Syncrip or mouse IgG; captured RNA was fragmented by MNase, then dephosphorylated and adapter‐ligated. RNA-seq: Total RNA was extracted using TRIzol (Invitrogen) from cultured NPCs. scRNA-seq: Single-cell suspensions were generated from dissected dorsal cortex, with no separate “extraction” step beyond cell dissociation. LACE‐seq: After reverse transcription, the first‐strand cDNA was barcoded and linearly amplified prior to high‐throughput sequencing. RNA‐seq: Libraries were prepared with NEBNext Ultra RNA Library Prep (paired-end, 2×150 bp). scRNA‐seq: Single‐cell libraries were generated using the 10x Genomics Chromium Single Cell 3′ Kit (v3.1), capturing ~6000 cells per channel.