title: ONT of SW1736
design: Three ug of gDNA from SW1736TERT/LUC cells was prepared (The Ramaciotti Centre for Genomics, Sydney NSW) as per manufacturer's protocol, with minor changes described below, using the Oxford Nanopore DNA by Ligation SQK-LSK114 kit (Oxford Nanopore Technologies, Cambridge, UK).Briefly, DNA was end repaired and dA-tailed using the NEB Companion module for ONT (New England Biolabs) at 20oC for 10 min and 65oC for 5 min. A 1x bead cleanup was performed with on bead incubation increased to 10 min. The elution step was performed for 10 min at 37oC. Adapter ligation was performed followed by 0.4x bead clean up with on bead incubation increased to 10 min. For the elution step, the beads were resuspended 26 uL elution buffer which was incubated for 30 min at 37oC. All hula rotation incubation steps were performed for 10 min.The library was sequenced (The Garvan Medical Research Institute, Sydney, Australia) on a R10.4.1 flow cell in high accuracy (400bps) mode on the ONT PromethION sequencing device (Oxford Nanopore Technologies