title: Single-cell RNA sequencing reveals immune microenvironment changes induced by Fe-Shikonin treatment in SCC7 tumors
design: Single-cell RNA sequencing reveals immune microenvironment changes induced by Fe-Shikonin treatment in SCC7 tumors
library_construction_protocol: Tumor tissues were collected from SCC7-bearing C3H mice following PBS or Fe-Shikonin treatment. Fresh tumor samples were excised and immediately processed for downstream single-cell preparation. Tumor-bearing mice were treated with either phosphate-buffered saline (PBS) or Fe-Shikonin according to the experimental design prior to tumor collection.  Single-cell suspensions were prepared from tumor tissues, and cellular RNA was captured during the droplet-based single-cell RNA library preparation workflow without bulk RNA extraction.  Single-cell RNA libraries were constructed using the DNBelab C Series High-throughput Single-cell RNA Library Preparation Set V3.0 (TaiM 4) according to the manufacturerâ€™s instructions. Briefly, single cells were partitioned into droplets, followed by reverse transcription, cDNA amplification, and library construction with sample-specific barcodes. 