title: Genome of Desulfovibrio sp 7SRBS1
design: The isolated bacteria Maridesulfovibrio_sp_2SRBS4, Maridesulfovibrio_sp_5SRBS1, Desulfovibrio_sp_7SRBS1were grown in SRBS broth (to 1 L: 2.32 g sulfate reducing broth, base, Millipore 28228,  10 g sodium thiosulfate, 4 ml of 60% sodium DL-lactate solution, 33 g instant ocean) at 25C for 5 days inside a glove box with an atmosphere of 80% N2, 10% CO2, 10% H2 . For DNA isolation: 1 ml of cells was harvested by centrifugation at 5,000  g for 10 minutes. Genomic DNA was obtained with the GeneJET Genomic DNA purification kit (Thermoscientific K0721) following the manufacturers user guide to purify genomic DNA from gram-negative bacteria. To measure the quality of the DNA, nanodrop was used. To quantify genomic DNA concentration, Qubit dsDNA HS assay kit (Invitrogen Q32851) was used. 20 l of 30 ng/l of DNA were sent for sequencing to eurofins