title: Anti-CD27 immunotherapy induces intratumoral immunostimulation in B-cell lymphoma: the multi centre RiVa trial (scRNA-Seq Data for RiVa)
design: Anti-CD27 immunotherapy induces intratumoral immunostimulation in B-cell lymphoma: the multi centre RiVa trial (scRNA-Seq Data for RiVa)
library_construction_protocol: Fresh cores of tumors cores collected in RPMI were minced into 1 mm3 pieces or smaller using a scalpel and added to Liberase™ DL (Roche, Basel) RPMI for 15 minutes at 37 oC on an orbital shaker at 250 rpm. The sample mixture was then passed through a 70 µm filter, washed four times, red cell lysis (Qiagen) undertaken, and washed twice more. Resultant single cell suspension was cryopreserved in 50% v/v human AB serum (Sigma, Burlington), 40% supplemented RPMI and 10% v/v DMSO (Sigma, Burlington). scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3' Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions. scRNAseq of pre- and on-treatment biopsies were performed using the 10X Genomics (Pleasanton, California) Chromium Single Cell 3' Library & Gel Bead Kit (chemistry version 3.1) as per protocol, from thawed single-cell suspensions.