title: Treatment group 4 microbial community
design: Fecal metagenomic sequencing of Pelophylax nigromaculatus. Total DNA was extracted from fecal samples and sequenced using paired-end Illumina NovaSeq 6000. The primary goal was to characterize the gut microbial community structure (taxonomic composition) and functional potential (via KEGG and GO pathways) of the host. Bioinformatics analysis included host sequence removal, de novo assembly, gene prediction, taxonomic classification, and diversity analysis (alpha and beta diversity) to compare microbial profiles.