title: WGS of Polish Konik horses: male whole blood
design: Libraries for WGS were constructed grouping 12 samples into four pooled sets to facilitate multiplex sequencing. Each prepared pool was subject to quality control, which included three main steps: concentration measurement (NanoDrop), size measurement (Bioanalyzer, Fragment Analyzer), and mimicking cluster creation (RT-PCR). The libraries were sequenced on the NovaSeq S4 Analyser (Illumina).