title: MH_Survey_2021_Soil
design: Soil samples were collected from six grasslands across northern California. Plots were dominated by either invasive Medusahead or one of two common local grasses, Avena barbata or Bromus tectorum. Soils were collected with a sterile soil corer (2.5cm diameter) to 10-15cm depth. Samples were homogenized and stored on dry ice until arrive in the lab where they were stored at -80. DNA was extracted with QIagen DNA PowerSoil kit following standard protocols. DNA extractions were standardied and amplified with dual-step PCR before purification with AMPure. Samples were pooled and sequenced on Illumina miseq 300bp PE