title: RNA-seq of Mucor circinelloides strain FRR5020 grown with varying calcium and phosphate availability
design: RNA-seq of Mucor circinelloides strain FRR5020 grown with varying calcium and phosphate availability
library_construction_protocol: Fungal mass was cultivated and collected according to Dzurendova et al. (2021), section 2.2: Dzurendova, S., Zimmermann, B., Kohler, A., Reitzel, K., Nielsen, U. G., Dupuy--Galet, B. X., . . . Shapaval, V. (2021). Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi. Journal of Fungi, 7(4), 300. https://doi.org/10.3390/jof7040300 RNA extraction was carried out using the RNeasy Plus Mini kit (Qiagen, Venlo, The Netherlands). Library preparation consisted of rRNA removal (polyA capture), followed by enrichment of single-stranded messenger RNAs (mRNAs) and reverse transcription to obtain complementary DNA (cDNA).