title: GSM8703378: ZF_Recorder-BoxB_N22-dreADARcd, rep_1, 4-hpf (RBPscan); Danio rerio; OTHER
library_construction_protocol: Total RNA from zebrafish embryos was extracted using TRIzol (ThermoFisher #15596026). Sequencing was performed on PCR amplicons of the 3' UTR of Recorder mRNA, either containing or lacking BoxB elements. The PCR reaction amplified the 3' UTR region of the Recorder mRNA, which includes the BoxB elements and the Recorder hairpin. Partial Illumina adapters present in the Recorder mRNA are complementary to sections of the i5 and i7 TruSeq Illumina primers, which also contain unique barcodes and sequencing adapters.