title: RNA-seq of maize seedlings with PEG treatment
design: Using fragmented mRNA as a template and random oligonucleotides as primers, the first strand of cDNA was synthesized in the M-MuLV reverse transcriptase system. Subsequently, the RNA strand was degraded using RNaseH, and the second strand of cDNA was synthesized from dNTPs in the DNA polymerase I system.