title: GSM8705492: Untreated 18day root replicate 2; Brassica rapa subsp. chinensis; RNA-Seq
library_construction_protocol: RNA was isolated from three independent biological replicates of whole roots and leaves separately of both consortia SAB treated and controlled on day 8 and day 18. Samples were snap-freeze by dipping them in liquid nitrogen and stored in a -80 deep freezer. After that samples were powdered with a mortar pestle in liquid nitrogen for RNA extraction using Qiagen Plant RNeasy extraction kit. RNA quantity was determined using Qubit fluorometer from Life Technologies with the "dsDNA BR Assay Kit" and RIN value (>8) by Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). oligo dT purification of RNA was followed by library preparation and subsequent sequencing of 40 million paired end 100bp reads/sample by Illumina HiSeq2500 at the SCLESE sequencing facility, Singapore.