title: GSM9268725: CP biol rep 1; Nicotiana benthamiana; RNA-Seq
library_construction_protocol: Total RNA was extracted from 8 mm leaf discs using the RNeasy plant mini kit (Qiagen) following the manufacturer's instructions. Total RNA (1 μg) from each sample was used for library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, E7420L) following the manufacturer's instructions. Prepared libraries were assessed for quality using NGS High-Sensitivity kit on a Fragment Analyzer (AATI) and for quantity using Qubit 2.0 fluorometer (Thermo Fisher Scientific).