title: WGS of Chakhao_ML: Control local black rice cultivar O. sativa ssp. indica cv. Chakhao of Manipur, India
design: The genomic DNA was cleaned-up using DNeasy PowerClean Pro Cleanup Kit. The purified genomic DNA was end-polished and A-tailed. The end-prepared samples were barcoded using Blunt/TA ligase master mix. The library was prepared by ligating sequencing adapters (SQK-NBD114.96) onto double-stranded DNA fragments using NEB Quick T4 DNA Ligase. After purification with AMPure XP beads, the prepared library was ready for library quality control.