title: GSM8774624: Vehicle_3; Mus musculus; RNA-Seq
library_construction_protocol: RNA-Seq library preparation and sequencing were conducted by Novogene (Beijing, CN) on a NovaSeq 6000 machine for IECs (EpCAM+CD45-) from wild type pups were sorted via BD FACS) ( pretreated with vehicle or PEox) The end reading of 150bp pairing was generated. RNA-Seq fastq files were first processed by fastp to remove adaptor and low-quality reads, then aligned to the reference genome using Hisat2 (v2.0.5).