title: GSM9013252: HumanLymphNode_H3K27ac CUT&Tag; Homo sapiens; OTHER
library_construction_protocol: The experimental protocol followed the detailed instructions for CUT&Tag tissue preparation (AXO-0456), FlowGel stamping and lysing (AXO-0457), and CUT&Tag library preparation (AXO-0460).  Protocols are available at https://www.atlasxomics.com/resources?category=Protocol#hello. On the glass slide, tissue is fixed, quenched, washed, permeabilized, and tagmented (AXO-0456). Using an AtlasXomics clamp, the FlowGel Chip, two barcode chips are applied perpendicular to each other to create a grid of paired barcodes spanning the region of interest (ROI), and each is ligated to the available DNA fragments at each spot (AXO-0457). The tissue is then lysed, and the DNA is purified. The DNA library is then prepared by PCR amplification, purification, and qualified prior to sequencing (AXO-0460).