title: shotgun metagenomics of Dermacentor reticulatus
design: dissected tick ovaries and Malpighian tubules were pooled, homogenized, filtered, and centrifuged to further extract the total genomic DNA from the obtained pellet using the NucleoSpin Soil kit (Macherey-Nagel). Libraries were prepared with Nextera DNA Flex (Illumina) and then sequenced using the Illumina NovaSeq 600 technology by MGX DNA Services Facility (Montpellier, France)