title: Reprogramming of androgen receptor activity in castration-resistant prostate cancer is shaped by truncated variants
design: Chromatin was obtained from the equivalent of 50 x 30 μm slices (AR) or 20 x 30 μm slices (H3K27ac). For AR, we used a pool of two antibodies for each ChIP: Abcam 74272 (3 μg) and Millipore PG-21 (2 μg). For H3K27ac, we used 2 μg of Abcam  4729 per ChIP.Libraries were prepared from 10 ng of DNA using an Ultra Low Library Preparation Kit (Qiagen), according to the manufacturer's protocol. Prepared libraries were sequenced on the NextSeq OH (Illumina) with single-end 75 bp sequencing at the South Australian Genomics Centre, targeting a depth of ≥25 million reads per sample