title: Metagenome sequencing of mouse gut microbiome
design: Fecal pellets were collected from cages stratified by human donor, intervention group, and sex, with one pellet per mouse sampled at each time point for DNA extraction and 16S rRNA sequencing. All samples were stored at 80C for subsequent microbiome profiling. Pellet samples were shipped to the University of Minnesota Genomics Center for further processing. DNA extraction from each sample was performed using Qiagen's DNeasy 96 PowerSoil Pro QIAcube HT Kit following the manufacturer's instructions. The V4 hypervariable region of the 16S rRNA gene was amplified using primers adapted from Gohl et al. (515F/806R), with Illumina adaptor overhang