title: GSM8745745: 5-HCT116_tetPADI4_Nutlin_ChIP_p53_dc_rep3; Homo sapiens; ChIP-Seq
library_construction_protocol: Non-histone samples were first crosslinked with EGS for 25 minutes. All cells were fixed with 1% formaldehyde for 10 minutes. After quenching and cell lysis, cells were sonicated with a Cavaris S220 Ultrasonicator. Barcoded libraries were made using NEB Ultra II DNA Library Prep Kit for Illumina following manufacturer's instructions and quantified by Agilent 4200 Tapestation System.