title: GSM8980139: Multiome, HAND sample2, GEX; Homo sapiens; RNA-Seq
library_construction_protocol: Flash-frozen human brain tissue samples were homogenized, and lysed and permeabilized with Nonidet P40 Substitute, Digitonin, and Tween-20. The isolated nuclei were purified, centrifuged, and resuspended in Diluted Nuclei Buffer. The nuclei were then loaded into the Chromium X (10x Genomics, Pleasanton, CA) to encapsulate single nuclei into droplet emulsions following the manufacturer's recommendations. 5000 nuclei were targeted to be captured per sample. Single nuclei gene expression libraries and single nuclei ATAC libraries were constructed from nuclei isolated from flash-frozen human brain tissue samples on the 10x Genomics Chromium System using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle.