title: GSM8978126: vtc2_Rep2; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: RNA from three independent replicates per genotype, where each replicate was composed of 8 randomized rosettes, was extracted with Trizol following the manufacturer's protocol. RNA quality and integrity were validated using the Bioanalyzer 2100 (Agilent Technologies Santa Clara, CA, USA), being the RNA integrity number (RIN) >8.0 for all biological replicates. Paired-end Illumina mRNA libraries were generated using the TruSeq stranded mRNA according to the manufacturer's instructions (Illumina Inc., San Diego, CA, USA) for the mutant and control lines. Libraries were sequenced in an Illumina NextSeq550 platform, and 2 x 75 bp paired-end reads were generated.