title: RNA sequencing data from Trypanosoma brucei brucei EATRO1125 before/after differentiation of bloodstream forms to procyclic forms
design: RNA sequencing data from Trypanosoma brucei brucei EATRO1125 before/after differentiation of bloodstream forms to procyclic forms
library_construction_protocol: Cells were cultured in DTM medium with 3 mM citrate and 3 mM cis-aconitate at 27oC. Total RNA samples were prepared using the Qiagen RNAeasy Mini Kit. RNA library construction was carried out using an the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina, with poly A selection of 1 microgram total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module, following the provided protocol.