title: GSM8616626: CUT&RUN for H3K27ac in N2a cells after transfection with the EV, replicate 3 (Bahcc1 OE control); Mus musculus; OTHER
library_construction_protocol: CUT&RUN reactions were performed as previously described (Meers et al., 2019), with the addition of 20mM sodium butyrate when profiling BAHCC1, SIN3A and H3K27ac RNA-seq libraries were prepared using the CORALL mRNA-Seq Kit V2 (Lexogen), following the manufacturer's instructions. CUT&RUN libraries were prepared as described previously (Meers et al., 2019)