title: BSseq of mTregs replicate 3
design: RRBS libraries from mTregs were prepared using the Zymo-Seq RRBS Library Kit (Cat. D5460), including MsP digestion, adapter ligation, bisulfite conversion, and indexed PCR per manufacturers protocol. Sequencing was performed on an Illumina NextSeq 2000 (50 bp paired-end).