title: GSM8980158: GSC2_atac; Homo sapiens; ATAC-seq
library_construction_protocol: For each biological replicate, 50,000 cells were used to prepare ATAC-seq libraries in accordance with the protocol used in Ackermann et al. for alpha and beta cells, which was adapted with some modifications from the original protocol from Buenrostro et al. and the Omni-ATAC protocol. Samples with low viability (85%) were treated with DNase (Worthington) at a concentration of 200 U/mL for 30 minutes at 37° C. Double-sided bead purification to remove primer dimers and large 1,000 bp fragments was carried out using Agencourt AMPure XP beads (Beckman Coulter). Quality control was performed before sequencing using a Fragment Analyzer (Agilent) and the KAPA Library Quantification Kit (Roche).