title: rRNA-Seq of Zebrafish Gut Microbiome (Control)
design: The zebrafish gut microbiota analysis involved DNA extraction, PCR amplification of 16S rRNA, purification, library preparation, and Illumina sequencing. Quality control ensured accurate data through DNA integrity checks, and sequencing produced high-quality reads. Bioinformatics tools like VSEARCH and PAST were used for OTU clustering, chimera detection, and diversity analysis. Taxonomic annotation provided insights into microbial diversity and abundance.