title: GSM8743661: E16.5M PGCs IUHG  bisulfite-seq bio rep3; Mus musculus; Bisulfite-Seq
library_construction_protocol: The EGFP-positive PGCs obtained through FACS were washed with PBS. DNA was isolated from the cells by using QIAamp® DNA Micro Kit (QIAGEN). Genomic DNA was subjected to bisulfite conversion using EpiArt® DNA Methylation Bisulfite Kit (Vazyme) following the manufacturer's instructions. Libraries for sequencing were prepared using 100ng-200ng of genomic DNA by EpiArt® DNA Methylation Library Kit. Libraries were purified for DNA fragments between 100bp-200bp. Library quality was assessed on the Agilent 5400 system (Agilent) and then amplified by PCR system.