title: GSM9008845: Participants 302,control, 12 months; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was prepared using the NucleoSpin RNA Mini Kit (Macherey-Nagel, Düren, Germany) following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNAsynthesis using either dUTP for directional library or dTTP for non-directional library