title: RNA-seq of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours
design: RNA-seq of 4T1 mouse breast cancer cells cultured on soft vs stiff polyacrylamide hydrogels for 24 hours
library_construction_protocol: 4T1 breast cancer cells were cultured on soft (1.24±1.3kPa ) and stiff  (13.20±2.3 kPa) two-dimensional polyacrylamide  hydrogels for 24hrs before being harvested for RNA extraction 4T1 breast cancer cells were cultured on stiff (13.20±2.3kPa ) two-dimensional polyacrylamide  hydrogels for 24hrs RNA extraction was performed using the Qiagen RNAeasy extraction kit as per manufacturers recommendations The quality of the RNA was confirmed using TapeStation (Agilent), prior to library preparation using the KAPA mRNA HyperPrep Kit, as per the manufacturer protocol. Quality control was performed on the mRNA library using a LabChip GX prior to RNA sequencing.