title: GSM9009430: Neural stem cells_ shKAP1 cells (1); Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Qiagen Mini RNeasy kit (Qiagene 74104). DNA was removed using RNase-Free DNase Set (Qiagen 79254). polyA RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP for strand specific library.