title: GSM9037115: 3m, scRNAseq; Mus musculus; RNA-Seq
library_construction_protocol: Proximal pulmonary arteries were excised with surrounding tissue. The arteries were mechanically chopped and placed in 1mg/ml collagenase (Roche) and 3 U/ml elastase (Worthington). Following cellular dissociation, we barcoded unique mRNA molecules of each cell using our 10x Genomics Chromium platform (3' v3.1 kit) and a droplet-based microfluidic system, then performed reverse transcription, cDNA amplification, fragmentation, adaptor ligation, and sample index PCR according to the manufacturer's protocol.