title: GSM9009007: MLO-Y4 cells, Hypoxia (2% O₂) for 24 hours, hypoxia_4; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy Mini Kit (Qiagen) following the manufacturer's instructions. To remove residual genomic DNA, RNA samples were treated with RNase-Free DNase Set (Qiagen). RNA quality was assessed using an Agilent 2100 Bioanalyzer, and only samples with an RNA Integrity Number (RIN) ≥8 were used for library preparation. RNA libraries for RNA-seq were prepared using the Illumina Stranded mRNA Prep, Ligation Kit  following manufacturer's protocol