title: DNA-Seq of Pasteurella multocida 203533
design: Long-read sequencing was performed on a Sequel IIe platform (2h pre-extension, 30h movie time), Libraris were prepared using the SMRTbell Prep Kit 3.0 (v07, sheared DNA to an average of 15 kb), Barcoded adapters were prepared with the SMRTbell Barcoded Adapter Plate 3.0., Primer annealing and polymerase binding were done with the Sequel II Binding Kit 3.2 according to the SMRT Link Sample Setup (SMRT Cell 8M Tray) and a Sequel II Sequencing Kit 2.0 was used for sequencing.