title: GSM9269219: RNA, Macrophage, Wild Type, LPS, 3h, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using AllPrep DNA/RNA Mini Kit (Qiagen, 80204) according to the manufacturer's instructions. 500 pg of RNA was used as input. Library preparation was conducted on 1 ng of cDNA using the Nextera XT DNA Sample Preparation Kit (Illumina) followed by SPRI (Beckman Coulter) size selection. Sequencing was performed by the Bio-medical Sequencing Facility at CeMM using the Illumina HiSeq 3000/4000 platform and the 50 bp single end configuration.