title: A high-resolution single-cell multiomic atlas of the human fetal lung - scRNA / GEX / VDJ
design: A high-resolution single-cell multiomic atlas of the human fetal lung - scRNA / GEX / VDJ
library_construction_protocol: Protocol carried out by Barker lab to extract an organ specimen from a paediatric donor Libraries were prepared according to the standard protocol of the Chromium 5'V1 VDJ kit. An in-house kit was used for gdTCR enrichment.  V(D)J libraries were prepared according to the manufacturerâ€™s protocol (10X Genomics) using individual Chromium i7 Sample Indices.5,000 PE reads per cell for V(D)J libraries were aimed for sequencing. Libraries for gamma/delta TCR variable regions were amplified as previously described (Mimitou et al. 2019)