title: GSM9285004: A. thaliana, S. indica-inoculated, 0 mM NaCl, biol rep 3; Arabidopsis thaliana; RNA-Seq
library_construction_protocol: Fungal-inoculated plant root samples were obtained at seven days post inoculation (dpi) and growth in the presence or absence of 50 mM NaCl. The first 4-5 cm of roots starting from the seed were excised and immediately frozen in liquid nitrogen. Either non-inoculated or fungal-inoculated plant root samples were crushed to a fine powder in liquid nitrogen with mortars and pestles. Total RNA was extracted with TRIzol reagent (Invitrogen) and on-column DNase digestion was performed according to manufacturer's protocol. RNA was purified by passage through Nucleospin RNA Plant columns (Mackerey-Nagel). Triplicates of control or the different saline treatments were used for the construction and analysis of RNA-seq libraries. The quality of RNA samples was checked in an Agilent 2100 Bioanalyzer. Those RNA samples with an RNA Integrity number (RIN) >7.5 and rRNA 28S/18S ratio >1.5 were selected for further analysis. Library construction and sequencing (150‑nt paired-end reads) on Illumina NovaSeqTM