title: GSM9269339: P0-hTSCs-EP300-1; Homo sapiens; OTHER
library_construction_protocol: For MeRIP-seq, total RNA was extracted with TRIzol reagent. CUT&Tag was performed according to the manufacturer's protocol (Vazyme, TD904). Nuclei were isolated, lysed, and incubated with Tn5 transposase according to the manufacturer's protocol (Vazyme, TD501). Transposed DNA was isolated using a DNA Clean & Concentrator kit (Zymo). For MeRIP-seq,the SMARTer Stranded Total RNA-Seq Kit-version 2 (Pico Input Mammalian, Takara/Clontech, 634413) was used to construct libraries for both the input and IP samples. For CUT&Tag, Library preparation and amplification was followed by manufacturer's protocol (Vazyme, TD904).