title: WGS of Homo sapiens
design: The process of whole genome sequencing (WGS) was as follows: genomic deoxyribonucleic acid (DNA) was extracted using the TANBead Maelstrom 4810 Automated DNA/RNA Purification Platform and the TANBead Nucleic Acid Extraction Kit Gram Negative Bacteria DNA Auto Plate (Taiwan Advanced Nanotech Inc.) following manufacturers guidelines. Following DNA extraction, the Nextera XT DNA sample preparation kit (Illumina San Diego, CA, US) was used to generate multiplexed paired-end DNA libraries. The Illumina sequencing chemistry uses DNA fragmentation of all input DNA, and then non-selective amplification of all DNA fragments present. Genome sequencing was carried out using an Illumina Nextseq 1000/2000 to generate 150 base pair paired-end reads. Cultures were stored at -80C in a medium containing skim milk, tryptone, glucose and, glycerol (STGG).