title: GSM9011790: TU212-2; Homo sapiens; miRNA-Seq
library_construction_protocol: The Small RNA Sample Pre Kit was used to construct the library. Taking advantage of the special structures at the 3' and 5' ends of small RNAs (with a complete phosphate group at the 5' end and a hydroxyl group at the 3' end), total RNA was used as the starting sample. Small RNAs were directly ligated with adapters at both ends, followed by reverse transcription to synthesize cDNA. Subsequently, cDNA was amplified by PCR, and target DNA fragments were separated by PAGE gel electrophoresis. The gel-cut and recovered products were the cDNA library.