title: GSM8835937: SVCs extracted from subcutaneous adipose tissue of wild type mice1 add seq1; Mus musculus; RNA-Seq
library_construction_protocol: ADSC was isolated from adult male mice. The mice were anesthetized and the subcutaneous adipose tissue was taken and rinsed in PBS for 3 times. The adipose tissue was cut into small pieces and digested at 37℃ for 40min with 0.075% Type Ⅷ collagenase. The cell suspension was filtered through a 100um filter and centrifuged at 1700rpm for 5min. After red blood cell lysis with 1×lysis buffer, cells were centrifuged and resuspended in growth medium (DMEM/F12 1:1 containing 1% Penicillin and streptomycin and 10% FBS) SVCs were loaded onto a GemCode instrument (10× Genomics) to generate single-cell barcoded droplets according to the manufacturer's protocol with the 10× single-cell 3' v2 chemistry