title: GSM9246334: Mandarin duck, male, early growth, in season, blue sail feather, epithelium, Rep 1; Aix galericulata; RNA-Seq
library_construction_protocol: Feather follicles were dissected into pulp (mesenchymal) and epithelium (epidermal) compartments. Total RNA was extracted using the TRIzol protocol (Invitrogen, Cat #15596026) following manufacturer's instructions. Libraries were prepared from 1 µg of total RNA per sample using the Illumina TruSeq RNA Prep Kit v2 (RS-122–2001/-2002). Poly(A)+ mRNA was enriched, fragmented, converted to cDNA, end-repaired, adapter-ligated, and PCR-amplified according to manufacturer's protocol.