title: GSM8703540: Tumor, Thbs2KO, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Tissues were first homogenized with TRIzol reagent (Thermo Fisher) and a beads homogenizer (Bertin Technologies Precellys 24 tissue homogenizer) and total RNA was extracted using the RNeasy Mini Kit (QIAGEN). Residual DNA was removed by on-column DNase digestion using the RNase-Free DNase Set (QIAGEN) . RNA libraries for RNA-seq were prepared with the TruSeq RNA Sample Prep Kit v2.