title: GSM9150138: pc293T, IgG Control, Replicate 2; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag was performed using the CUT&Tag-IT® Assay Kit (Active Motif # 53160). The manufacturer protocol was followed with the application of the following antibodies:  DYKDDDDK Tag (EpiCypher #13-2031), H3.3 (EpiCypher # 13-0061), and Rabbit IgG (Cell Signaling #2729S). Each target was run in triplicate with 2x105 pcHEK293T cells per sample. 20ul of CUT&Tag-IT Spike-In Control, Anti Rabbit nuclei were added as normalization control (Active Motif #53168) Libraries were prepped as described in manufacturer protocol for CUT&Tag-IT® Assay Kit (Active Motif # 53160).