title: ATAC-seq of spleen B cells from B cell-specific Zeb2-CKO and WT mice
design: ATAC-seq of spleen B cells from B cell-specific Zeb2-CKO and WT mice
library_construction_protocol: Splenic B cells from B-cell specific Zeb2-CKO mice and WT mice were purified by negative selection and were induced in the presence of R848, anti-CD40,anti-IgM F(ab')2 , IL-21, IFN-γ for 3 days, then, cells were collected. Briefly, 50,000 live cells were freshly isolated and resuspended in lysis buffer (10 mM Tris·Cl,（pH 7.4）,10 mM NaCl，3 mM MgCl2，0.1% (v/v) Igepal CA-630) and pelleted. The cell pellet was resuspended in transposase reaction mix (5×TTBL 10μl, TTE Mix V50 5μl, ddH2O 35μl) and incubated at 37°C for 30 min. Fragmented DNA was purified using VAHTS DNA Clean Beads and library was generated by PCR.