title: GSM9011292: S34F_NO_DOX_VEHICLE_2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated from sorted GFP+ mouse MLL::AF9 BM cells after SMG1i-63 or vehicle treatment using the Quick-RNA MicroPrep Kit (Fisher-Zymo, No. R1050). Quality of isolated RNA was determined using Agilent RNA 6000 Nano chip using the Agilent 4200 TapeStation platform. Quantification was performed using Qubit Fluorometer (Thermo-Fisher Scientific). Total RNA samples with RNA integrity number (RIN) >9 were selected for Illumina sequencing library preparation. For K562 same protocol was used for library preparation. The RNA-sequencing libraries were constructed using KAPA RNA HyperPrep Kit with RiboErase (Roche, Catalog # KK8560/61), per the manufacturer's protocol. After amplification, final libraries (~360 bp mean size) were sequenced on Illumina NovaSeq 6000, generating 2 x 150bp paired-end reads.