title: 12S MiFish metabarcoding of PCR grade water: Laboratory control sample
design: PCRgrade water serving as a library prep control was amplified with MiFish metabarcoding primers (Miya et al. 2016) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.