title: GSM8704884: SW1736-sh.RBM10-A, Replicate1 RBM10-KD; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was extracted from the 5 isogenic RBM10 overexpressing or KD thyroid cancer cell lines using the Qiagen RNeasy extraction kit 500 ng of total RNA with RQN values of 9.1-10 underwent polyA selection and TruSeq library preparation according to instructions provided by Illumina (TruSeq Stranded mRNA LT Kit, catalog # RS-122-2102), with 8 cycles of PCR.  Samples were barcoded and run on a HiSeq in a PE125 run, using the HiSeq 3000/4000 SBS Kit (Illumina).  An average of 100 million paired reads was generated per sample.