title: RNA-Seq of Root Tip
design: The RNA-Seq experiment aims to profile gene expression across multiple tissues of Brainea insignis, an endangered fern species, for genome annotation purposes. RNA was extracted from six different tissues: gametophyte, crisped,leaf, young leaf, mature leaf, root tip, and stem. Total RNA was isolated using a standard RNA extraction kit (Qiagen RNeasy), with quality verified using a Bioanalyzer. PolyA-selected mRNA was used for library construction.