title: Transcriptome analysis of compound-treated cells
design: Transcriptome analysis of compound-treated cells
library_construction_protocol: Huh7.5.1 cells were seeded in 12-well plates, with three replicates for each treatment. Cells were incubated for 48 h with LY-411575, JNJ-1802, NITD-688, and NITD-008 at their respective EC90 concentrations against DENV: 10 nM, 0.1 nM, 7 nM, and 1.3 Î¼M. Cells treated with DMSO served as controls. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, #74106). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using either dUTP for directional library or dTTP for non-directional library.