title: GSM9246692: Human skin biopsy_15; Homo sapiens; OTHER
library_construction_protocol: DNA was extracted from two 20 m FFPE sections using the truXTRAC FFPE Total Nucleic Acid Plus Kit (Covaris Ltd., Woburn, Massachusetts, USA). Concentrations were measured with a Qubit fluorometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA). DNA fragmentation was performed using Adaptive Focused Acoustics Technology from Covaris according to the manufacturer's instructions. DNA quality was assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, California, USA). Libraries were prepared using 100 ng of DNA and TruSight Oncology 500 kit (Illumina Inc., San Diego, California, USA), involving A-base addition, adapter ligation, and hybridisation with probes targeting 523 genes. Library concentrations were measured fluorometrically using a Qubit fluorometer, and sequenced on an Illumina NextSeq 500 (Illumina) using paired-end 101-base reads, generating fastq files.