title: RNA-seq of brain tissues: 6-8 weeks male rats
design: All experimental data was processed by GraphPad Prism 8.0 software (GraphPad Prism Software Inc., San Diego, CA, USA) and presented in the form of means  standard deviation (SD). T-tests were used for comparisons between two groups. One-way analysis of variance (ANOVA) was used for comparisons among multiple groups, followed by Tukeys post hoc test. p < 0.09 indicated that the difference was statistically significant.