title: CRISPRi-seq of Haemophilus influenzae in BHI and CDM
design: A tetracycline-inducible H. influenzae RdKW20 CRISPRi library was used to evaluate the essentialome in 2 growth media, after which bacteria were collected followed by genomic DNA isolation, amplicon library construction and sequencing as described in https://pubmed.ncbi.nlm.nih.gov/34997243/