title: GSM8980060: Liquid culture from yeast with duplication of chr7 after seven days transitioning to hyphae at 25C, replicate C; Histoplasma ohiense (nom. inval.); RNA-Seq
library_construction_protocol: Cells were collected by filtration, flash frozen in liquid nitrogen, and stored at -80°C prior to RNA extraction. Libraries for RNAseq were prepared using the NEB Next Ultra II Directional RNA Library Prep Kit (New England Biolabs, Ipswich, MA). Average fragment size and presence of excess adapter was determined with High Sensitivity DNA Bioanalyzer chip from Agilent Technologies (Santa Clara, CA). Library concentration was measured by Qubit dsDNA BR Quantification Assay Kit (Invitrogen) then pooled with equal DNA mass from each library. The final libraries were submitted to the UCSF Center for Advanced Technology for sequencing on an Illumina Novaseq X 10B sequencer.