title: GSM8647031: Duodenum, HFD, KO4; Mus musculus; RNA-Seq
library_construction_protocol: Frozen tissues were homogenized and total RNA was extracted by TRIzol method, the integrity was assessed by Bioanalyzer 2100 system. mRNA was purified using poly-T oligo-attached magnetic beads. NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530) was used for library preparation following manufacturer's recommendations.