title: GSM9247477: Ampullary carcinoma patient 3; Homo sapiens; RNA-Seq
library_construction_protocol: After thawing, cell suspensions were filtered (40 µM), followed by two sequential washing steps (RPMI-1640 medium + 10% FBS), and resuspended in cold PBS 1X. Cell suspensions of approximately 20,000 cells were obtained, and cell viability was manually evaluated using trypan blue staining. Only samples with more than 50% cell viability were further used for a droplet-based single-cell capture protocol. Samples were prepared following the user guide “Chromium Next GEM Single Cell 3′ Reagent Kit v3.1 (Dual Index)” and loaded into the Chromium X (10x Genomics).