title: Metagenomic WGS sequencing of Human : Oral microbioime
design: DNA libraries were prepared using the Nextera XT DNA Library Preparation Kit(Illumina) and IDT Unique Dual Indexes with total DNA input of 1ng. Genomic DNA wasfragmented using a proportional amount of Illumina Nextera XT fragmentation enzyme.Unique dual indexes were added to each sample followed by 12 cycles of PCR toconstruct libraries. DNA libraries were purified using AMpure magnetic Beads (BeckmanCoulter) and eluted in QIAGEN EB buffer. DNA libraries were quantified using Qubit 4fluorometer and Qubit dsDNA HS Assay Kit