title: metataxonomic sequencing of bioreactor sludge
design: NA was extracted using the DNeasy Power Soil kit (QIAGEN, Hilden, Germany) and its concentration and purity measured using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific) and BioDrop LITE nano-spectrophotometer (Biodrop, UK), respectively. All the samples were stored at -20C . DNA was sequenced commercially using the platform Illumina NovaSeq 6000, and the libraries were prepared by a two-step PCR employing the Nextera technology targeting the 16S rRNA genes V4 and V5 hypervariable regions using the primers 515F (5-GTGYCAGCMGCCGCGGTAA-3) and 926R (5-CCGYCAATTYMTTTRAGTTT-3). A mock community consisting of 10 bacterial strains was included as a positive control