title: Shotgun metagenomics of the human fecal virome
design: Starting from human feces sample, viral particles were enriched using ultracentrifugation and treated with nucleases. RNA and DNA were extracted using the PureLink Viral RNA/DNA Mini Kit. RNA was reverse transcribed and second-strand synthesis of cDNA was performed. Both DNA and ds-cDNA were amplified using SISPA. Library was prepared using the DNA Prep (M) Tagmentation Kit and sequenced in an Illumina MiniSeq (PE150 reads).