title: GSM9247524: X4DF1cc_39; Bos taurus; RNA-Seq
library_construction_protocol: using miRNeasy Mini Kit (QIAGEN, Hilden, Germany), RNase-Free DNase Set (QIAGEN, Hilden, Germany; cat #79254) SMART-Seq HT Kit (Takara Bio Inc), following the manufacturer's recommendation.cDNA was purified using AMPure XP Beads (Beckman Coulter, the Qubit dsDNA High Sensitivity (ThermoFisher Scientific) and Bioanalyzer High Sensitivity DNA Kit (Agilent) were used to determine the cDNA concentration and profile, respectively. The Nextera XT DNA Library Prep (Illumina) was used to prepare the libraries as recommended by the SMART-Seq HT kit.