title: GSM9246492: HFF KO HCMV GFP1, rep1; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from each sorted population, ancestor samples, and samples without HCMV infection using the NK lysis protocol (DOI: 10.1016/j.cell.2015.02.038). Genomic DNA was used as a template for PCR to amplify the sgRNAs, as described in (DOI: 10.7554/eLife.58461). Here, we used 1ug of genomic DNA for the first PCR reaction and ran the PCR program for 16 cycles. Shifted primers were used to increase library complexity.