title: GSM9043546: 06_ID-38-PAM2-3; Homo sapiens; RNA-Seq
library_construction_protocol: Cell were harvested after a 5–10-minute incubation with 1 mL of 0.25 % trypsin-EDTA, further neutralized with 4 mL of 2 % v/v fetal bovine serum in filter sterilized phosphate-buffered saline (PBS). Detached cells were centrifuged at 250 g for 5 minutes at room temperature (25 ºC) and the resulting cell pellet was reconstituted in 10 mL of GM. Cell counting was performed using a 200 µL sample in a Scepter 3.0 handheld automated cell counter (60 µm sensors). A frozen pellet of 1,000,000 cells per sample was prepared and sent according to Active Motif for their end-to-end RNA-seq service according to manufacturer's instructions. Isolation of total RNA, sample quality/integrity assessment, PolyA mRNA enrichment, directional library preparation (stranded method), QC of NGS library, and sequencing using Illumina platform were performed using proprietary standard protocols and analysis by Active Motif.