title: GSM8775910: hCMEC/D3 cells, 0 μM rutin, 24 h, bio rep 1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted via TRIzol reagent, and RNA integrity was assessed using the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification. The library was checked with Qubit and real-time PCR for quantification and bioanalyzer for size distribution detection.