title: M. smegmatis INH one step selection line G colony 16
design: DNA was extracted from cell pellet using the QIAGEN DNeasy UltraClean Microbial Kit as per manufacturer protocol. 1 ng of purified genomic DNA was prepared into sequencing libraries using the Illumina Nextera XT DNA library prep kit with the Nextera XT Index Kit v2. The completed libraries were quality checked using the Agilent Tapestation and quantified with the Qubit 1x dsDNA high sensitivity kit prior to equimolar pooling to 2 nM and loading onto a NextSeq P2 300 cycle flowcell (PE 150) at 750 pM with 1% PhiX.