title: GSM8618514: 812-6 HuLM RNA-seq_HuLM_VEH_rep2_JQ1 RNA; Homo sapiens; RNA-Seq
library_construction_protocol: We performed whole transcriptome analysis to determine the mechanism  underlying the inhibitory effect of BET protein inhibition on the uterine fibroid cells. The HuLM cells  were treated with BET protein inhibitors JQ1 and I-BET 762 at a concentration of 5 µM  for 48 h. RNA was isolated using Trizol. RNA quality and quantity were assessed using  the Agilent bio-analyzer. Strand-specific RNA-Seq libraries were prepared using a  TruSeq total RNA-Seq library protocol (Illumina provided). Library quality and quantity  were assessed using the Agilent bio-analyzer, and libraries were sequenced using an  Illumina NovaSEQ6000 (illumine provided reagents and protocols).