title: GSM8978691: C.necator, ammonium sulfate, 30h, biol rep1; Cupriavidus necator; RNA-Seq
library_construction_protocol: The RNA was extracted using RNeasy protect bacteria mini kit (Qiagen), 1ug of total RNA was used for the construction of libraries. Firstly, ribosomal RNA was removed from total RNA, followed by ethanol precipitation. After fragmentation, the first strand cDNA was synthesized using random hexamer primers. During the second strand cDNA synthesis, dUTPs were replaced with dTTPs in the reaction buffer. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion, amplification, and purification.