title: RNAseq_dko
design: Total RNA was extracted by using the RNeasy Lipid Tissue Mini Kit (Qiagen) and the RNA quality was analyzed by using a 5200 Fragment Analyzer System and Agilent HS RNA kit (Agilent Technologies). Libraries were prepared by using MGIEasy RNA Directional Library  Prep Set (MGI Tech). The libraries were further certificated by a Quit 3.0 Fluorometer and dsDNA HS Assay  Kit (Thermo) and a Fragment Analyzer and dsDNA 915 Reagent Kit (Aglilent). The dsDNA libraries were converted to ss circular DNA libraries by using the MGIEasy Circularization Kit (MGI Tech), and DNA nanoballs were prepared by using DNBSEQ D 400 RS High Throughput Sequencing Kit (MGI Tech) and sequenced by using a DNBSEQ G400 (MGI Tech) with 2 x150 bp paired-end reads. Adaptor sequences and low-quality sequences were removed by using Cutadapt v1.9.1.