title: GSM9149598: Experiment 1, Primary mouse astrocyte, 6 hour treatment, S3QEL1.2, rep1; Mus musculus; RNA-Seq
library_construction_protocol: Cultured primary cells were rinsed once with ice-cold PBS and collected in freshly prepared extraction buffer consisting of 1% β-mercaptothanol in RLT (Qiagen). Total RNA was extracted using the RNeasy Mini Kit with on-column DNase treatment (Qiagen). RNA libraries were prepared using Truseq Standard mRNA Sample Prep (Poly A selection) following manufacturer's instructions (Document #1000000040498v00).