title: Genome of Pseudodesulfovibrio sp 7M6
design: The isolated bacteria Pseudodesulfovibrio_sp_7M6 was grown in MBS broth (to 1 L: 37.4 g Marine broth Difco 2216 , 4 ml of 60% sodium DL-lactate solution, pH 7.2-7.6) at 25C for 5 days days inside a glove box with an atmosphere of 80% N2, 10% CO2, 10% H2. For DNA isolation: 1 ml of cells was harvested by centrifugation at 2,500  g for 5 minutes. The cell pellet was resuspended in 500 l of extraction buffer (10 mM Tris-HCl pH 8.0, 10 mM EDTA pH 8.0 and 2% SDS). Proteinase K (20 mg/ml) (ThermoScientific, AM2542) was added to the solution to a final concentration of 770 g/ml. Samples were mixed by inversion and incubated at 56C for 3 h. DNA was extracted by standard phenol:chloroform extraction using equal volume phenol: chloroform: isoamyl alcohol (25:24:1) (ThermoScientific, 327111000). DNA was precipitated with 1/10 volume of 3 M sodium acetate buffer solution pH 5.2 (ThermoScientific, R1181) followed by the addition of 2.5 volumes of ice-cold absolute ethanol. Samples were mixed and stored at -20 overnight. To recover the DNA, the samples were collected at 13000 x g for 30 min at 4C. Supernatant was discarded. Then, 70% ethanol was added to each tube. DNA was collected by spinning at 13000 x g for 30 min at 4C. Supernatant was discarded. The DNA pellet was allowed to dry and finally, it was resuspended in 30 l of 10 mM Tris buffer, pH 8.0.To measure the quality of the DNA, nanodrop was used. To quantify genomic DNA concentration, Qubit dsDNA HS assay kit (Invitrogen Q32851) was used. 20 l of 30 ng/l of DNA were sent for sequencing to eurofins