title: Metabarcoding bacterial and fungal communities of adult wild bees
design: Qiagen Blood & Tissue kits (catalogue #69506) were used to extract DNA destructively from the whole body of each sample following protocols described in Nguyen et al. (2023). Metabarcoding was conducted with two target loci selected to represent bacteria (16S rRNA) and fungi (ITS1). The 799F-mod3-M13F and 1115R-M13R primers were used for 16S (CMGGATTAGATACCCKGG and AGGGTTGCGCTCGTTG), while ITS1 was amplified with ITS1-F-M13F and ITS2-M13R (CTTGGTCATTTAGAGGAAGTAA and GCTGCGTTCTTCATCGATGC). Each extracted DNA sample was sequenced with three replicates for redundancy, which were later combined. Library preparation, amplification, sequencing, and demultiplexing steps were all conducted at the Centre for Biodiversity Genomics in Guelph, Canada on an Illumina MiSeq system.