title: GSM8647210: RL3_HF_Chr; Rattus norvegicus; RNA-Seq
library_construction_protocol: RNA was harvested with phenol / chloroform Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers, followed by the second strand cDNA synthesis using dUTP  instead of dTTP. The directional library was ready after end repair, A-tailing, adapter ligation, size selection, USER enzyme digestion to remove UTP-containing second strand cDNA, amplification, and purification.