title: RNA-Seq of Penaeus vannamei
design: The mRNA (with PolyA tail) was enriched by Oligo(dT) magnetic beads, and the mRNA obtained was randomly interrupted by divalent cations in Fragmentation Buffer. cDNA was synthesized using the fragmented mRNA as a template, and PCR amplification was carried out after performing end-repairing, connecting to sequencing junctions, and adding A tails to the cDNA. The resulting cDNA library underwent quality control before being sequenced using Illumina NovaSeq 6000