title: Mesaconate Attenuates LPS/D-GalN-Induced Acute Liver Failure by Enhancing HIF-1α-Mediated AXL-Dependent Efferocytosis
design: Mesaconate Attenuates LPS/D-GalN-Induced Acute Liver Failure by Enhancing HIF-1α-Mediated AXL-Dependent Efferocytosis
library_construction_protocol: WT BMDMs were inoculated in 6-well plates. First, they were pretreated with 10 mM itaconate or mesaconate for 4 hours, then treated with 10ng/ml LPS for 3 hours. After that, the culture medium was discarded and 1ml of RNAiso Plus was added to each well. The samples were stored at -80℃ and quantitatively analyzed using the sequencing platform of the Beijing Institute of Genomics. RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kitfollowing manufacturer's protocols.