title: GSM8703514: Hep_5_1;8hr; Homo sapiens; RNA-Seq
library_construction_protocol: Cells from the control, 0R, and 8R conditions were sent to Novogene (Sacramento, CA) for RNA isolation, library preparation, and sequencing. Total RNA was isolated using the Qiagen RNeasy series kit, and quality control (QC) was meticulously performed at each step to ensure reliable data. For transcriptome sequencing, 1 μg of total RNA per sample was used to generate sequencing libraries with the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer's protocol. Libraries were purified with the AMPure XP system and validated on the Agilent Bioanalyzer 2100 system.