title: SH-RGS1-2
design: Total RNA is extracted from cells, reverse transcribed into mRNA, and subsequently separated and fragmented. Reverse transcriptase utilizes mRNA as a template to synthesize the first strand of complementary DNA (cDNA), while the second strand is synthesized and the mRNA is degraded, resulting in the production of double-stranded cDNA (ds cDNA), which is then purified. The double-stranded cDNA undergoes end-repair and end-filling, followed by purification of the repaired cDNA. Adapters are then ligated, the library is enriched through PCR, and sequencing is conducted after passing quality inspection.