title: GSM8978302: Vehicle-2; Homo sapiens; RNA-Seq
library_construction_protocol: PDX subcutaneous mice were given a single dose of vehicle, or PM 10 mg/kg, when tumor size reached around 500 mm3. Tumor tissues were collected for bulk RNA-seq after 24 hours. Total RNA was extracted using the Trizol method. Total RNA was extracted using the Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's protocol. RNA purity and quantification were evaluated using the NanoDrop 2000 spectrophotometer (Thermo Scientific, USA). RNA integrity was assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Then the libraries were constructed using VAHTS Universal V6 RNA-seq Library Prep Kit according to the manufacturer's instructions.