title: GSM9282631: Germline specific over-expression of EcR-B1; Drosophila melanogaster; RNA-Seq
library_construction_protocol: 100 ovaries were dissected in Grace's insect medium, disassociated in a solution containing 0.5% type 1 collagenase and 1% trypsin in PBS for 15 minutes, which was stopped by adding Grace's insect medium with 10% FBS. Suspended cells were passed through a Flowmi 40-micron filter and  then centrifuged at 5 rpm for 3 minutes. Single cells were resuspended and washed with 0.04% BSA in PBS, centrifuged again for collection of single cells. Single Cell 3' v2 protocol (10x Genomics)