title: GSM8646897: R21007149-WT-WT-1; Mus musculus; RNA-Seq
library_construction_protocol: the lungs were resuspended with PBS containing 0.1 % Collagenase type I (SCR103, Sigma-Aldrich), 0.05 % DNase I (D5025, Sigma-Aldrich), and 5 % FBS (13011-8611, Tianhang Biotechnology). After incubation at 37 °C for 60 min, the samples were pestled and filtrated with a 200-mesh sieve, pelleted (300 g, 5 minutes), and resuspended in ACK red blood cell lysis buffer (Gibco A1049201) for 3 minutes, after which the buffer was inactivated by adding excess processing buffer. Cells were then filtered through a 70 μm strainer, pelleted again (300 g, 5 minutes), and resuspended in PBS buffer, counted, and immediately processed for single-cell RNA sequencing. scRNA-seq libraries were constructed according to Singleron GEXSCOPE protocol by GEXSCOPE Single-Cell RNA Library Kit (Singleron Biotechnologies)