title: GSM8776331: A11, Normoxia, Rep3; Homo sapiens; RNA-Seq
library_construction_protocol: The plates were incubated at 4ºC for 1 h and then washed four times with ice-cold PBS to give a pellet of neurospheres. RNA was isolated using a QIAshredder spin column (Qiagen). RNA was further isolated using AllPrep DNA/RNA Mini Kit (Qiagen). For library preparation, the Illumina Truseq stranded mRNA kit was used, and paired end read sequencing was performed on a NovaSeq 6000 machine