title: RNA-seq of cotton
design: Disease resistance tests were conducted on two varieties of cotton, A and B. Samples were taken at 0, 24, 48, and 72 h, followed by transcriptome sequencing. Three biological replicates were set up, totaling 24 samples. The transcriptome sequencing analysis was entrusted to Beijing Bemac Biotechnology Co., Ltd. for RNA extraction and quality testing of cotton leaves, construction of sequencing libraries and quality testing, and transcriptome sequencing of the samples using the Illumina HiSeq 2500 sequencing platform.