title: GSM9362654: MR_174; Homo sapiens; miRNA-Seq
library_construction_protocol: miRNA was extracted from biopsies or brushing samples using the miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions. miRNA sequencing libraries were prepared using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEBioLabs). Sequencing adapters that target the 3' hydroxyl group of small RNAs were ligated and the transcripts were reverse transcribed and PCR-amplified into single-stranded cDNA libraries. The libraries were pooled, and size selected for small RNA fragments on PAGE gel in groups of 6-10.