title: GSM9270733: JJ0388, control, Refeeding; Mus musculus; RNA-Seq
library_construction_protocol: At the pre-determined brain collection points, mice were euthanized with carbon dioxide followed by rapid decapitation. The brains were immediately dissected and split into two hemispheres. One hemisphere from each brain was placed in liquid nitrogen then stored at −80 °C. In total, 48 half brains were sent to AmpSeq for RNA extraction and mRNA sequencing (poly-A selection, 20 million reads). RNA-seq libraries were prepared using the VAHTS Universal V10 RNA-seq Library Prep Kit (Illumina-compatible). Library concentration was quantified with Qubit and qPCR, and fragment size distribution was evaluated using Qseq.