title: GSM9271064: HPAFII_Control_8h_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from HPAF-IIcell lines using the Maxwell® RSC simplyRNA Cells Kit. cDNA libraries were prepared according to the SMART™-seq2 protocol, which utilizes a template-switching oligonucleotide (TSO_II) for full-length cDNA synthesis. RNA-seq libraries were prepared using 50 ng of purified cDNA with the Illumina DNA Prep Kit. The protocol included tagmentation, post-tagmentation cleanup, PCR amplification, and pooling of indexed libraries. Final libraries were then normalized to 4 nM. Library denaturation and dilution were performed following the Illumina NextSeq System Denature and Dilute Libraries Guide.