title: HIF2A(EPAS1) Genome-Wide Localization in MRC5 Human Fibroblasts by Cut & Tag
design: HIF2A(EPAS1) Genome-Wide Localization in MRC5 Human Fibroblasts by Cut & Tag
library_construction_protocol: Trypsin digestion of attached cells and binding of 100,000 cells to magnetic concanavalin beads for Cut & Tag Proliferating cells in DMEM + Gluta-Max + 10% fetal bovine serum HIF2A over-expression versus HIF2A endogenous expression (pBabe control) Phenol-chloroform extraction after Cut & Tag. AMPure after PCR amplification of libraries. PCR amplification with Illumina Nextera indexed adaptors