title: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
design: Enhancement of colorectal cancer therapy through interruption of the HSF1-HSP90 axis by p53 activation or cell cycle inhibition
library_construction_protocol: RNA samples were generated as pools. In detail, two biological replicates, each consisting of two pooled biological replicates per treatment group (total n = 4 per group; DMSO, Ganet, RG-7388, combi) were analyzed. The cells were cultured in RPMI 1640 supplemented with 10 % fetal bovine serum, glutamine and penicillin/streptomycin in a humidified atmosphere at 37°C with 5 % CO2. HCT116 cells were treated for 24 hrs with DMSO, 50 nM Ganetespib, 1 µM RG-7388 or in combination. The TRIZOL reagent from Invitrogen has been used for RNA extraction. Library construction performed by NovogeneNEB Next® Ultra™ RNA Library Prep Kit Library quality control: Agilent bioanalyzer 2100, DNA high-sensitivity chip Concentration checked by qPCR.