title: GSM8979128: liver, CFm3; Mus musculus; RNA-Seq
library_construction_protocol: Approximately 10 mg of liver tissue (left lobe) was immersed in 600 μl TRIzol, homogenized thoroughly, snap-frozen in liquid nitrogen, and stored at -80°C for RNA extraction the following day. All samples were ground using a high throughput tissue grinder at 300 g for 30 s (repeated three times). Following centrifugation at 14,000 g at 4°C for 10 min. RNA was extracted using M5 HiPer Universal Plus RNA Mini Kit (Mei5 Biotech, Beijing, China) according to manufacturer instructions. All RNA samples used had a RIN >8. All RNA samples were sent to Novogene Co., Ltd (Beijing, China) for quality control (Sample QC). After RNA library preparation, cDNA libraries are sequenced on Illumina NovaSeq platforms using a paired-end 150 bp sequencing strategy. Approximately 6 G clean data and a >75% uniquely mapped reads rate for each sample were obtained for each sample. Six biological replicates were included.