title: RNA-sequencing of scrambled control CIN 612 cells and CIN 612 cells depleted of senataxin (SETX) through stable expression of shRNAs
design: RNA-sequencing of scrambled control CIN 612 cells and CIN 612 cells depleted of senataxin (SETX) through stable expression of shRNAs
library_construction_protocol: NIH-3T3 J2 cells were removed through treatment with versene for >5min. Primary cells were then scraped and harvested on ice. RNA was extracted using the Qiagen RNeasy Mini Kit. At least 1ug of RNA was submitted per sample. RNA libraries for RNA-seq were prepared using the DNBseq Eukaryotic Strand-specific Transcriptome Resequencing; stranded mRNA library (HNO2D).