title: GSM8908005: PBS; Mus musculus; RNA-Seq
library_construction_protocol: Single-cell suspensions were prepared according to the protocol of Chromium Single Cell 3'Solution (V3 chemistry). All specimens were washed two times with cold 1x phosphate-buffered saline (PBS) .Haemocytometer (Thermo Fisher Scientific) was used to evaluate cell viability rates.Then, we used Countstar to count the concentration of sinale-cell suspension and adiust the concentration to1000 cells/ul. Samples that were lower than the required cell concentration defined in the user guide (i.e., <400 cells/ul) were pelleted and re-suspended in a reduced volume, and then the concentration of the new solution was counted again. Single-cell RNA sequencing (scRNA-seq) libraries were constructed using the 10x Chromium Single Cell 3' platform (10X Genomics, Pleasanton, CA, USA) according to the manufacturer's protocol. Briefly, single-cell suspensions were loaded onto a Chro-mium microfluidic chip to generate single-cell Gel Beads in Emulsion (GEMs). The scRNA-seq libraries were then prepared using the Chromium Single Cell 3' Reagent Kit v3.1 (10X Genomics, Pleasanton, CA, USA). Library quality was assessed using the Ag-ilent 2100 Bioanalyzer system (Agilent Technologies, Santa Clara, CA, USA), and se-quencing was performed on the Illumina NovaSeq 6000 platform (Illumina, San Di-ego, CA, USA) with a sequencing depth of at least 50,000 reads per cell.