title: 3dFn2 (CRX1465797; CRA022397)
library_construction_protocol: Messenger RNA was purified from total RNA using poly-T oligoattached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.