title: Metagenome sample from mouse cecum
design: The genomic DNA from the mouse feces was extracted using QIamp PowerFecal Pro DNA Kit (Qiagen, Hilden, Germany) according to the manufacturers protocol. The V3-V4 hypervariable regions of the16S rRNA gene were amplified via PCR with the following conditions: initial denaturation at 95 C for 3 min; denaturation at 95 C for 30 s, annealing at 55 C for 30 s, and extension at 72 C for 30 s, repeated for 25 cycles; and final extension at 72 C for 5 min [38]. The amplicons were purified using a gel and PCR clean-up kit (Macherey-Nagel, Dueren, Germany), and were then used to construct sequencing libraries using the Illumina TruSeq DNA Sample Preparation Kit (Illumina, CA, USA). Finally, amplification and sequencing of the V3-V4 region of 16S rRNA gene was performed by on Illumina Miseq platform (MiSeq Reagent Kit v2)