title: RNA-seq of Sau65_R1
design: Exponential cultures of S. aureus were grown on TSB at 37C. After 3 hours, total RNA was extracted using the RNeasy mini-kit (Qiagen) followed by a DNAse treatment with the TURBO-DNA free kit (Thermo Fisher Scientific). RNA library preparation was performed using the Illumina Stranded Total RNA Prep Ligation with Ribo-Zero Plus kit and 10bp unique dual indices. Sequencing was done on a NovaSeq X Plus (SeqCenter, USA), producing paired-end 150bp reads.