title: Whole-genome sequencing of Rhizobium pusense strain KSSKSLAB04 using PacBio Onso and Oxford Nanopore PromethION
design: Genomic DNA from Rhizobium pusense strain KSSKSLAB04, a plasticizer-degrading bacterium isolated from soil samples polluted with plastic waste at Juhu Beach, Mumbai, India, was sequenced using a hybrid long-read approach. Library preparation for short-read, high-accuracy sequencing was performed using the QIASeq FX DNA Library Prep Kit, and the resulting libraries were converted to PacBio Onso-compatible format using the Onso Library Conversion Kit. Long-read Nanopore libraries were prepared using the ONT Native Barcoding Kit and sequenced on the PromethION24 platform. The objective of this experiment is to generate high-quality whole-genome sequencing data to support a hybrid de novo assembly of the R. pusense strain. Authors: Khushboo Sharma (Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India), Dr. Anuraj Nayarisseri (In silico Research Laboratory, Eminent Biosciences - EMBS, Indore, Madhya Pradesh, India), Prof. Sanjeev Kumar Singh (Department of Bioinformatics, Alagappa University, Karaikudi, Tamil Nadu, India).