title: GSM9246458: Lung tissue from TMAO-treated LPS-ALI mouse3; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from platelets using Trizol Reagent (Invitrogen, USA).To enrich for messenger RNA (mRNA), ribosomal RNA (rRNA) was depleted using the rRNA Depletion Kit v2 (E7400L, NEBNext, MA, USA). RNA sequencing libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit for Illumina Kit (E7770s, NEBNext, MA, USA) and quantified on an Agilent 4200 Bioanalyzer (Shanghai, China) following manufacturer's protocols.