title: 16S rRNA gene amplicon of bovine: rumen liquid
design: Rumen samples were collected by using stomach tube. Samples were aliquoted, snap frozen in dry ice and stored at -80 oC until DNA extraction. Total DNA was extracted using Qiagen Dneasy Power Soil Pro kit. For bacterial amplicon sequencing universal primers 515f/806r targeting 16S rRNA gene V4 region were used.