title: 16S rRNA gene metabarcoding of Nile tilapia mid intestine fed with autochthonous probiotics
design: DNA was extracted from the mid intestine of Nile tilapia using QIAamp PowerFecal Pro DNA Kit following the procedure in the manufacturers handbook. The PCR amplification of the 16S V3-V4 region using specific primers (CCTAYGGGRBGCASCAG,GGACTACNNGGGTATCTAAT), and the high-throughput sequencing was performed by Novogene Company Limited, United Kingdom