title: RNA-Seq of ATLT cell (shNC)
design: Total RNA was extracted from HeLa S3 cells transfected with pX1MT-M-WT after 48 h. Library preparations were conducted using a mix of 250ng total RNA and 0.25 ng Spike-in RNA Variant Control Mix E2 (Lexogen, Austria) with the TruSeq RNA Library Prep Kit (Illumina). The ready-to-sequence libraries werequantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries(Kapa Biosystems, USA), and The ready-to-sequence libraries were quantified by qPCR using the KAPA Library Quantification Kit for Illumina Libraries (Kapa Biosystems, USA), and library profiles were evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, USA).