title: GSM9040649: IL1b, High, Rep2; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted using Quick-DNA FFPE Kit (Zymo Research). PCR reactions containing up to 10 μg genomic DNA in each reaction were performed using ExTaq DNA Polymerase with primers to amplify the sgRNAs. Samples were then purified with SPRIselect beads, mixed and sequenced on a NextSeq 500 by 75-bp single-end sequencing.