title: RNA-Seq of Nlug: adult female
design: Library constructed from ovaries of 1-day post-emergence adult female Nilaparvata lugens (brown planthopper):1. Total RNA extracted using TRIzol Reagent (Invitrogen) following the manufacturers protocol;2. RNA integrity verified via 1% agarose gel electrophoresis, and RNA concentration quantified using a spectrophotometer;3. Enriched RNA fragmented, reverse-transcribed to cDNA, and ligated with Illumina-compatible adapters;4. cDNA fragments with an insert size of ~200 bp amplified by 12 cycles of PCR;5. Final library sequenced on the Illumina NovaSeq 6000 platform with paired-end 154 bp reads.