title: RNA-seq sequence from the transcriptome for CureGN sample KF1498
design: Blood samples were collected in PAXgene Blood RNA Tubes. RNA samples were extracted using the QuickGene RNA Blood Cell Kit S (Catalog # RB-S) and further purified with the Zymo Research RNA Clean & Concentrator-5 Kit (Catalog # R1014). Poly-A pull-down was used to enrich mRNAs from the total RNA samples with an RIN greater than 8 (200 nanogram to 1 microgram per sample), followed by library preparation using the Illumina TruSeq RNA Prep Kit. Sequencing was carried out on the Illumina NovaSeq 6000 platform, generating 100 bp paired-end reads.