title: GSM9270673: PA1 structures, wild-type mice, E9.5-E9.75 (25 to 27-somite stage) 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using trizol method. All the RNA samples were assessed for their integrity using Qsep400 instrument. 1 μg of total RNA was used for the construction of sequencing libraries. RNA-seq were performed by Wuhan IGENEBOOK Biotechnology Co.,Ltd (http://www.igenebook.com) . RNA libraries for RNA-seq were prepared using the VAHTS universal V10 Library Prep Kit for Illumina(NR606,Vazyme). The procedure included polyA-selected RNA extraction, RNA fragmentation, random hexamer primed reverse transcription.