title: GSM9246338: Mandarin duck, male, early growth, in season, blue+brown sail feather, H3K27ac, Rep 1; Aix galericulata; ChIP-Seq
library_construction_protocol: Feather follicles (sail and adjacent flight feathers) were dissected. Chromatin was crosslinked, isolated, and sheared. Immunoprecipitation was performed with anti-H3K27ac antibody (Abcam # ab4729), alongside input controls. ChIP and input DNA were end-repaired, A-tailed, adapter-ligated, size-selected, and PCR-amplified using Illumina-compatible ChIP-seq protocols. Libraries were sequenced on Illumina HiSeq/NextSeq platforms.