title: GSM8980041: Promoter gRNA MPA R2 [CUT&RUN]; Homo sapiens; OTHER
library_construction_protocol: Targeted DNA amplicons were isolated using the Epicypher CUTANA Cut&Run Kit Version 3 with antibodies targeting PGR and control antibody IgG. PGR Cut&Run was conducted using a 1:25 dilution of the PGR antibody (Santa Cruz; Cat. No. sc-7208) incubated in antibody incubation buffer overnight at 4C. Targeted DNA amplicons were purified using the Qiagen QIAquick PCR Purification Kit (Qiagen, Hilden, Germany). Sequencing libraries were prepared using the NEBNext Ultra II DNA library preparation kit for Illumina (New England Biolabs, Ipswich, MA, USA), with 13 cycles of PCR amplification. The libraries were sequenced by the NIEHS sequencing core.