title: RNA-seq of 786-O cells for control
design: Total RNA was extracted using TRIzol. High-quality RNA was used for library construction with polyA selection. Libraries were sequenced on an Illumina NovaSeq 6000 (paired-end, 350550 bp insert). Raw reads were trimmed with TrimGalore and mapped to hg19 using HISAT2. Gene expression was quantified with StringTie and differential expression analyzed with DESeq2.