title: Shotgun metagenomics sequencing of whole fecal DNA from ALL pediatric patient
design: Microbial DNA was extracted from fecal samples and quantified with a Qubit fluorometer. Libraries were prepared using the QIAseq FX DNA Library Kit (Qiagen) following the manufacturers instructions, then sequenced as 2  150-bp paired-end reads on an Illumina NovaSeq platform.