title: RNA-Seq of Felis catus: obese early male
design: Genomic DNA was extracted using the PureLinkTM Microbiome DNA Purification kit (Invitrogen), and its quality was verified by spectrophotometry and agarose gel electrophoresis. Sequencing was performed on the Illumina iSeq platform (2  150 bp) after amplification of the V4 region, purification with MagBio beads, indexing with Nextera XT V2, and verification with the Agilent Bioanalyzer. The sample pool was normalized and combined with Phix phage for sequencing.