title: GSM9285100: Mouse E10.5 forelimbs, Tbx5 ChIP-Seq replicate 2; Mus musculus; ChIP-Seq
library_construction_protocol: Forelimb samples were crosslinked with 1% formaldehyde, and the tissues were pre-disrupted with a probe sonicator (Branson 450) before shearing in a Bioruptor. Sheared chromatin was incubated in Protein G Agarose Columns (Active Motif) with a human/mouse TBX5 polyclonal antibody (R&D Systems AF5918) ChIP-seq libraries were prepared using the NEBNext Ultra II Library Prep Kit Sera-Mag SpeedBeads were utilized to perform size selection and to remove primer and adapter fragments Libraries were sequenced by the Georgia Genomics and Bioinformatics Core to generate single-end 75 bp reads.