title: GSM8880322: FKS3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from Huh7 cells using Trizol reagent and quality-checked using NanoDrop One. RNA-seq library preparation and sequencing were performed by Shanghai Majorbio Bio-pharm Biotechnology Co., Ltd. using the Illumina Stranded mRNA Prep protocol. Total RNA (1μg) was poly(A)-selected, fragmented, and converted to double-stranded cDNA using random hexamer primers. Libraries were prepared through end-repair, A-tailing, size selection (~300bp), and PCR amplification (15 cycles), followed by paired-end sequencing (2×150bp) on the NovaSeq X plus platform.