title: scRNA-seq of TBT-04301021
design: Single cell RNA-seq libraries were created from BAL cells as per manufacturers instructions using 10x Genomics single cell RNA 5 prime kits (v1.1). The target number of cells per channel was 5000 and two channels were used per sample (with the pooled count of 10,000 cells sequenced). Libraries were sequenced on a Novaseq 6000 S4 flowcell. Accepted minimum sequencing depth was 30,000 mean reads per cell and 50% sequencing saturation.