title: EMT6 Balbc Allograft-mouse3
design: Female Balb/c mice were inoculated with EMT6 cells into the mammary fat pad. After 15 days, the mice were euthanized by cervical dislocation. Tumor tissues were collected, immediately snap-frozen in liquid nitrogen, and stored at -80C until further analysis. Total RNA was then isolated from the samples for RNA-seq.