title: WGS of RPE1  SMARCA4 KO1 parental
design: Genomic DNA was extracted using phenol/chloroform and precipitated in ethanol, then quantified using Qubit. 1 g of DNA per sample was used as input material for sequencing library preparation. Libraries were created using the NEBNext DNA Library Prep Kit following manufacturer's recommendations. Genomic DNA was fragmented to 350bp by shearing. DNA fragments were end polished, A-tailed, and ligated with the NEBNext adapter for Illumina sequencing. Fragments were amplified by PCR with the P5 and indexed P7 oligos. Amplified libraries were purified with the AMPure XP system, then analysed for size distribution using the Agilent 2100 Bioanalyzer and quantified using real-time PCR. Libraries were sequenced on the Illumina Novaseq 6000 with 150150bp reads.