title: Whole genome sequence of Pseudomonas chlororaphis subsp. aureofaciens ATCC 13985; phz operon deletion
design: Genomic DNA was isolated from overnight LB cultures with BioBasic One-4-All kit. Oxford Nanopore sequencing was performed by Flow Genomics service. Library preparation used the Rapid Barcoding Kit 96 version 14 (SQK-RBK114.96), following the manufacturer's instructions, with an R10.4.1 flow cell. Sequencing was performed on an Oxford Nanopore GridION with basecalling using the Sup v5.0.0 model.