title: GSM8587265: Infected RAW264.7, 0.1% DMSO, rep1, run1; Mus musculus; RNA-Seq
library_construction_protocol: Infect RAW cells with JE2-Lux at MOI 20 in 4 mL fresh MEM in 6-well plates for 35 min. Replace with 4 mL MEM containing 100 ug/mL gentamicin and 40 uM KL1 or 0.1% DMSO for 24 h. Wash the cells with 4 mL PBS and harvest the cells by EDTA dissociation. Wash three times in 10 mL PBS to remove excessive EDTA at 4 C. Resuspend in 1 mL PBS, transfer to microcentrifuge tubes and pellet the cells. Remove the supernatans and store at -80 C. Submit samples to Azenta Life Sciences RNA libraries for RNA-seq were prepared based on the standard protocol by Azenta Life Sciences