title: GSM9042996: smad1-KD_PGC_1dpf_rep2; Danio rerio; RNA-Seq
library_construction_protocol: Full-length cDNA from FACS-sorted PGCs was generated using the Single Cell Full Length mRNA-Amplification Kit (Vazyme, catalog no. N712), according to the manufacturer's manual. For library preparation, 1 ng of cDNA was processed with the TruePrep DNA Library Prep Kit V2 (Vazyme, TD503), where adaptors were inserted via transposase reaction and tagged fragments were amplified.