title: GMC_077_NPC
design: After cell lysis and cytosol removal, nuclei were treated with Tn5 enzyme for 30 minutes at 37°C and purified with Minelute PCR Purification Kit to produce tagmented DNA samples. Tagmented DNA was barcoded with Nextera Index Kit v2 and amplified via PCR prior to a SPRI Bead cleanup to obtain purified DNA libraries.