title: GSM8616820: Nr_cond_Control_rep3_lamina; Nepenthes rafflesiana; RNA-Seq
library_construction_protocol: We conducted total RNA extractions using the Spectrum Plant Total RNA kit, according to manufacturer's protocol with some modifications. First, the leaves were transferred into clean tubes and centrifuged at max speed for 30 seconds to remove remnant RNALater. For the mechanical lysis step, we used a single large steel bead (autoclaved and sterilized with RNAse Away) to homogenize the leaf sample in lysis buffer using a FastPrep machine set to 5.5 m/s for 20 s. Following homogenization, the sample was incubated in a heatblock at 56 C for 5 minutes, and the remaining steps proceeded as per the original protocol. Our extracted RNA was sent to Novogene for library prep, quality control, and sequencing on an Illumina NovaSeq 6000 PE150 platform.