title: 16S amplicon of root endosphere: VOL control T=0_1
design: Metagenomic DNA was extracted from soil, and the rhizosphere and root endosphere of Medicago truncatula plants exposed to Sinorhizobium meliloti volatilomes or pure methylketones. This metagenomic DNA was used to create bacterial amplicon libraries of the V3-V4 region of the 16S rRNA gene which were sequenced 2 x 275 bp using Illumina technology. Prior to library preparation for root endosphere samples, PCR clamps were used to limit plant chloroplast and mitochondria amplification.