title: GSM8808870: Bulk RNAseq, E15.5 back skin from 1 control embryo, SAMPLE 2; Mus musculus; RNA-Seq
library_construction_protocol: Back skin from E15.5 mouse embryos (3 control and 3 Prmt5 cKO embryos) was dissected and immediately transferred to ice cold DEPC-PBS (Millipore Sigma, D5758). RNA was isolated using the RNeasy Fibrous Mini Kit (Qiagen, 74704) following manufacturer's instructions. cDNA libraries were generated using the Illumina TruSeq Stranded mRNA kit and samples sequenced using the NovaSeq6000 platform (~30 million, 200 bp, paired-end reads/sample).