title: Effect of the combined neonatal pneumonia virus of mice infection and house dust mite exposure on the trancriptional profile of dendritic cells in the lung
design: Effect of the combined neonatal pneumonia virus of mice infection and house dust mite exposure on the trancriptional profile of dendritic cells in the lung
library_construction_protocol: Single cell suspensions of the lung were obtained and cells were stained for 30 minutes at 4 degrees celcius before sorting. Following sorting strategy was used: live cells, CD45 IV negative cells. For each sample, 7500 immune cells were sorted (CD45+) and 7500 structural cells (CD45-, CD31-, Ter119-, EPCAM+) Single live cells were sorted and loaded onto a Chromium GemCode Single Cell Instrument (10x Genomics) to generate single-cell gel beads in emulsion (GEMs) The scRNA-Seq libraries were prepared using the GemCode Single Cell 3' Gel Bead and Library kit, version NextGEM 3.1 (10x Genomics) according to the manufacturer's instructions