title: GSM9284991: OE19_Methylprednisolone_2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using the Qiagen RNeasy Mini Kit following the manufacturer's instructions, including on-column DNase digestion to remove genomic DNA contamination. RNA quantity and integrity were assessed by fluorometric quantification and Agilent Bioanalyzer analysis prior to library preparation. RNA-seq libraries were prepared by the University of Notre Dame Genomics Core using standard poly(A) mRNA-seq workflows. Libraries were constructed according to Illumina protocols and sequenced on the Illumina NextSeq 2000 platform using paired-end 50 bp (PE50) reads, targeting approximately 30 million reads per sample.