title: WGS of Pseudomonas aeruginosa bloodstream infection
design: DNA extraction with the EZ1 Advanced XL system (Qiagen, Hilden, Germany) according to the manufacture instructions. The quantity of the extracted DNA was measured using a Qubit 3.0 fluorometer with the double-stranded DNA (dsDNA) assay kit (Thermo Fischer Scientific, MA, USA). Extracted DNA was diluted to an approximate target sample concentration of 10 nanogram/microliters and a target sample volume of 50 microliters