title: miRNA Seq of homo sapiens: follicular fluid
design: . After final purification, library quantification and quality control were performed using QuantiFluor ONE dsDNA System on Quantus. Quality verification was carried out by Bioanalyzer using the Agilent High Sensitivity DNA Kit. Multiplexed samples (1.2 pM) were sequenced as 75 singleread cycles on a MiniSeq System. Data analysis was performed on GeneGlobe using the RNA-seq Analysis & Biomarker Discovery Pipeline. FASTQ files were aligned against the reference database miRBase_v22, Homo sapiens (GRCh38.103) in the RNA-seq Analysis Portal 4.0 to obtain total count sequence reads.