title: Genomic DNA of Cochliomyia hominivorax: female parent
design: DNA will be extracted using the NucleoMag Tissue Kit and IsoPure robot for Illumina library prep and sequencing. After collecting purified DNA in 0.2 ml tube strips.  Purity was checked using Nanodrop (results in data table below).  Twenty-five microliters of the samples highlighted in red were cleaned using Amicon Centrifugal devices (protocol below).  Three washes with 500 l of water were completed, and samples were re-assayed with Nanodrop.  Samples were stored overnight at 4C.  One additional was was completed on those highlighted in red and samples were re-assayed.  1:5 diluted cleaned samples were quantified on the Qubit using the protocol below.