title: 16SrRNA_human_feces
design: The dataset from **MiSeq Run #1031** was sequenced with a **500-cycle kit v2** on a **Nano flowcell** (251 cycles, 250 nt reads). A **Fluidigm library** was prepared, quantified via qPCR, and demultiplexed using **bcl2fastq v2.20**. **PhiX DNA** served as a spike-in control, with most reads removed during processing. Fastq files use **Sanger quality scores** (ASCII offset 33).