title: GSM9268516: Panc02, allograft 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA from positively sorted NK cells was extracted using Dynabeads™ Oligo(dT)25 RNA isolation kit (Thermo Fisher Scientific) according to the manufacturer's instructions. Quality of RNA was assessed by agarose gel electrophoresis. Reverse transcription was performed with a reverse transcription system (Promega). Total RNA from cell experiments or allograft tumors was isolated by TriZOL® (Thermo Fisher Scientific) according to the manufacturer's instructions as described before (29). For allograft tumors, tissue was dispersed using an Ultra Turrax (IKA®). RNA integrity was determined by the RNA Pico kit using a Bioanalyzer 2100. Samples with RIN values between 8.5 and 10 were considered. The isolated RNA served as a template for RNA sequencing. Libraries for RNA sequencing were conducted according to the manufacturer's instructions (Illumina). For a total of 100 ng (cell culture) or 500 ng (allograft), polyA enrichment with oligo dT beads was performed, before strand-specific libraries were generated using Stranded mRNA Prep kit (Illumina).