title: Nup 98 Spleen  CT EG - F1 2024
design: RNA was extracted from bone marrow and spleen samples of transgenic mice using the RNeasy Mini Kit (Qiagen, Hilden, Germany). RNA quantity was measured with a NanoDrop-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), and quality was assessed by chip-based electrophoresis using the Bioanalyzer 2100 System (Agilent Technologies, Palo Alto, CA, USA). FASTQ files were generated after sequencing on the Illumina NovaSeq S1 platform.