title: Leishmania donovani 1S Axenic Amastigote
design: 7-day differentiated axenic amastigotes culture were isolated using Trizol extraction. One microgram of total RNA was then used to prepare Oxford Nanopore Technology (ONT) Direct RNA sequencing library using the SQK-RNA002 kit (ONT).  Induro (NEB) reverse transcriptase was used to synthesize complementary cDNA (to remove secondary RNA structures and improve sequencing). Each library was then sequenced on a R9.4 flow-cell until exhaustion