title: GSM9282597: Dicer1+/D1693N cells, cultured in Mesencult, passage 15; Mus musculus; RNA-Seq
library_construction_protocol: RNA from frozen cell pellets was extracted using the Zymo Quick-RNA Miniprep Kit (Zymo cat# R1054). Total RNA integrity is assessed with the TapeStation 4200 (Agilent Technologies; RNA Assay Cat No.5067-5576) and quantified with the Qubit RNA HS assay (ThermoFisher; Cat No. Q32852).  Total RNA is used as input to enrich for mRNA with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB; Cat No. E7490). Strand-specific libraries are prepared using the DNBSEQ Fast RNA Library Prep kit (Complete Genomics; Cat No. 940-001522-00) and sequenced at Vancouver Prostate Centre. Library quality and size are checked with the TapeStation 4200 (Agilent Technologies; D1000 Assay Cat No. 5067-5582) and then quantified using the Qubit dsDNA HS Assay (ThermoFisher; Cat No. Q32854). The libraries are sequenced using a Complete Genomics DNBSEQ-G400RS High-throughput Sequencer to generate 30 million 100-bp paired-end reads per library. 