title: GSM8705443: primary mouse brain microvascular endothelial cells-DTG treated-2; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated from cell samples using TRIzol reagent (Invitrogen). A total amount of 2 μg of RNA was used for construction of sequencing libraries RNA quality was first assessed by Agilent Fragment Analyzer, then was subjected to library preparation using the Illumina TruSeq Stranded mRNA Library Preparation Kit (RS-122-2101) according to the manufacturer's instructions.