title: GSM8777858: mRNAseq_N2_25h_Rep2; Caenorhabditis elegans; RNA-Seq
library_construction_protocol: For RNA extraction, samples were submitted to 4 rounds of freeze-thawing, and then RNA was purified using the Direct-zol RNA microcprep kit (R2062, Zymo Research) according to manufacturers instructions. Libraries were prepared using the TruSeq Illumina mRNA-seq (stranded - high input) protocol.