title: GSM9269753: HEK293T MELL3/14 KD, DMSO-treated, RNA-Seq, rep2; Homo sapiens; RNA-Seq
library_construction_protocol: Cells were chilled on ice and washed with PBS supplemented with cycloheximide (100 µg/ml), lysed with buffer containing 20 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, 100 µg/ml cycloheximide, and 25 U/ml TURBO DNase (Ambion). The lysate was divided into two parts for Ribo-Seq and RNA-Seq library preparation. RNA-Seq libraries were constructed using a NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB). The rRNA depletion was performed using a RiboMinus™ Eukaryote Kit v2 (Thermo Fisher Scientific) according to the manufacturer's recommendations.