title: RNA-seq analysis of PBMCs with TLR1-1805TT and TLR1-1805GG genotypes stimulated and restimulated (innate immune tolerance induction) with B. burgdorferi.
design: RNA-seq analysis of PBMCs with TLR1-1805TT and TLR1-1805GG genotypes stimulated and restimulated (innate immune tolerance induction) with B. burgdorferi.
library_construction_protocol: Cells were harvested following stimulation (16hr) and restimulation (6hr) for total RNA extraction. PBMCs were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) in humidified atmosphere with 5% CO2 at 37C. Control cells were given growth medium at stimulation and restimulation. Stimulated and Restimulated cells were treated with live B. burgdorferi. Total RNA was extracted using Rneasy Plus Micro Kit (Qiagen). Libraries were prepared using NEBNext Ultra-II Directional RNA Library Prep Kit (Illumina).