title: GSM8703684: U251MG N2CR 3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated using TRIzol Reagent following the manufacturer's protocol. The quality of total RNA was evaluated using an Agilent Tapestation 4200, and only samples with an RNA Integrity Number (RIN) above 9.0 were selected for RNA-seq library preparation with the Illumina® Stranded mRNA Prep kit (Illumina, San Diego, CA). Library preparation was conducted according to the manufacturer's protocol by the UCSD Institute for Genomic Medicine (IGM) Core Facility.