title: GSM8745541: invitro KPN DCAplus, rep1; Mus musculus; RNA-Seq
library_construction_protocol: RNA-seq experiments were performed on 3 independent replicates. RNA was extracted and purified using RNeasy Mini Kit(Qiagen, Cat:74104). The samples were further purified using the RNA Clean & Concentrator-5 Kit (Zymo Research, R1013). 1 ug of total RNA was used for the construction of sequencing libraries.RNA quality and quantity were assessed using the Agilent bio-analyzer. RNA-SEQ libraries were prepared using a TruSEQ based mRNA-SEQ library protocol (Illumina). Library quality and quantity was assessed using the Agilent bio-analyzer and libraries were sequenced using an Illumina NovaSEQ6000 (Illumina provided reagents and protocols).