title: GSM9247176: Blood, ALFQA, Week 24 Day 2, 14U044; Macaca mulatta; RNA-Seq
library_construction_protocol: Total RNA was extracted from 10,000-100,000 or 30,000-6.5 million cryopreserved PBMC using the Single Cell RNA Purification Kit (Norgen Biotek Corp) or the PureLink RNA Mini Kit (ThermoFisher Scientific) Amplified cDNA was made from 6.66-10ng of RNA using the SMART-Seq v4 Ultra Low Input RNA kit (Takara Bio Inc.), spiked with 5μL of 1:20,000 diluted ERCC Mix 1 or 2 (ThermoFisher Scientific). Sequence-ready libraries were generated using the Illumina DNA Library Prep Kit, quantified on a MiSeq.