title: RNA-seq for al
design: two groups of yellowfin seabream (infection group and control group), with 120 fish per group, were selected and divided into three replicates. The infection group (SI) was intraperitoneally injected with 0.1 mL of the LD50 of SI, while the control group (PBS) was injected with an equal volume of PBS. After 76 hours, the liver was dissected and collected. Three fish were pooled as one sample, and three samples were taken from each group.