title: GSM9013469: BIN67-CnR IgG-1; Homo sapiens; OTHER
library_construction_protocol: For RNA-seq, RNA was extracted from 2-3 million cells using Trizol and the Direct-zol RNA mini prep kit by Zymo Research. For CUT&RUN, 500,000 cells were bound to ConA beads and processed following the Cell Signaling CUT&RUN kit protocol. For MYC and IgG three separate reactions, equivalent to 1.5 million cells, was pooled together during the DNA purification step before library generation. For H3K4me3, one reaction was sufficient to achieve signal. For RNA-seq, libraries were made at Novogene using their standard methods with polyA-enrichment. For CUT&RUN, we made libraries of purified DNA fragments using the NEBNext Ultra II DNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina.