title: RNA sequencing of src-1(cj293) mutants
design: RNA was isolated from mixed-stage animals as previously described. Poly-A selection and RNA seq library construction was conducted using the NEBnext stranded RNA seq kit. RNA seq libraries were made using the NEBNext stranded mRNA library kit. Sequencing was conducted on a Nextseq 2000 instrument with 150-bp paired-end sequencing.