title: 16S amplicon sequencing of marine finfish gut mucosa: Diet Treatment - 50% Black Soldier Fly Meal; Tank Number - T-6; Replicate - a
design: Based upon GENEWIZs procedure: 16S-EZ rDNA next generation sequencing library preparations and Illumina MiSeq sequencing were conducted at GENEWIZ, Inc. (South Plainfield, NJ, USA). Sequencing library was prepared using a MetaVx 16s rDNA Library Preparation kit (GENEWIZ, Inc., South Plainfield, NJ, USA). Briefly, the DNA was used to generate amplicons that cover V3 and V4 hypervariable regions of bacteria and archaea16S rDNA. Indexed adapters were added to the ends of the 16S rDNA amplicons by limited cycle PCR. DNA libraries were validated and quantified before loading. The pooled DNA libraries were loaded on an Illumina MiSeq instrument according to manufacturers instructions (Illumina, San Diego, CA, USA). The samples were sequenced using a 2x 250 paired-end (PE) configuration. Image analysis and base calling were conducted by the Illumina Control Software on the Illumina instrument.