title: Metagenome of microcosm biofilm: Allulose treated for 43 h replicate 1
design: DNA was extracted from microcosm biofilm samples using the FastDNA Spin Kit. The V3V4 region of the 16S rRNA gene was amplified with primers 341F and 805R using Q5 Hot Start Polymerase. PCR products were pooled, purified with AMPure XP beads, and equimolarly combined for library construction. Sequencing was performed on the Illumina MiSeq platform with 2  262 bp paired-end reads.