title: GSM9012044: AGS, sgEmpty, H3K36me3, Dox D4, ChIP; Homo sapiens; ChIP-Seq
library_construction_protocol: Cells were fixed with 1% formaldehyde for 10 min. For SETD1A, RNAP2, Ser2P and H3K36me3 ChIP, we fixed cells with 2 mM DSG for 30 min before formaldehyde fixation. After washing and cell lysis, nuclei pellets were sonicated by using Picoruptor (Diagenode) Libraries were prepared using the KAPA Hyper Prep Kit (KAPA Biosystems).