title: GSM9268499: AngII; Mus musculus; OTHER
library_construction_protocol: The HiChIP-seq protocol was conducted and H3K27ac (Invitrogen, PA5-85524, 9 μL per ChIP) antibody was used in vehicle control and Klf5 inhibitor ML264-pretreated mouse VSMCs under AngII treatment (10-7 mol/L). Briefly, cells were cross-linked in 1.5% formaldehyde and were pelleted, washed and centrifugation. Capture HiChIP processing FASTQ files (paired-end Illumina data) were processed with the HiC-Pro v2.11.1 using Bowtie2 v2.3.5.1 for mapping short reads to the reference genome mm10 / hg19. After the mapping, filtering and deduplication steps of the HiC-Pro pipeline, normalized matrices were generated at 500 kb, or 10 kb resolutions. A large number of loop structures can be identified by Fit HiChIP analysis software.