title: GSM9247757: Blank, S. aureus infectious cranial defect, Day 14, Sample 2; Rattus norvegicus; RNA-Seq
library_construction_protocol: Two weeks post-implantation, the rats were euthanized, and tissues from the defect regions were carefully dissected, rinsed with pre-chilled sterile PBS, blotted dry, and immediately transferred into cryovials containing RNA-later (5 mL, Beyotime). Samples were snap-frozen in liquid nitrogen and sent to Suzhou Genewiz Biological Technology Co., Ltd. (China) for high-throughput RNA sequencing and subsequent bioinformatics analysis. Use NR605 kit - select rRNA removal product by species, remove rRNA, do strand-specific construction. It combines second-strand synthesis and end repair dA-Tailing, taking 3h. Or use #NR616, premixed for Illumina/MGI, reducing steps and time.