title: GSM9009180: SNU-449; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA from parental (untransfected) SNU cell lines and Huh7 cells transfected with pT-HBV1.3 was isolated using TRI reagent according to the instructions of the manufacturer. Up to 1 µg of extracted total RNA was used further for generation of individual libraries. RNA-seq (using poly(A) selection) was conducted on individual libraries generated using total RNA isolated from parental SNU cell lines or from transfected Huh7 cells on Illumina NovaSeq 6000 Sequencing System at the Genomics Core of the University of Kansas Medical Center (KUMC).  Library preparation used the TECAN Universal Plus stranded (fr-secondstrand) mRNA-Seq kit, and sequencing was conducted with 100-cycle paired-end reads. Each sample yielded between 33.8 and 42.8 million reads, with a median of 38.5 million reads. Quality assessment with the FastQC software (60) showed that all samples had an average Phred score above 30. Sequencing reads were trimmed with TrimGalore (61)  to remove low-quality and spurious sequences.