title: RNA-Seq of DBV: clinical isolates from acute DBV patients in China
design: For deep sequencing library preparation, viral RNA extracted from patient serum was reverse transcribed using random hexamers. Following DNase treatment and purification, second-strand cDNA synthesis was performed. Libraries were prepared using Nextera XT reagents (Illumina), which involved fragmentation and adapter ligation in a single step through tagmentation process. The resulting libraries were purified and enriched by limited-cycle PCR before sequencing on the NovaSeq 6000 platform.