title: Sample at 95 days of bioreactor with 30 C temperature 18S
design: Genomic DNA was extracted from 0.25 g of granular sludge from reactors at 20 C and 30 C on days 11, 95, and 165 using the Allprep PowerViral and QIAamp PowerFecal PRO-DNA kits. DNA concentration was measured with a Qubit fluorometer, and the V4 regions of the 18S rRNA genes were amplified by PCR, purifying products of 400450 bp. Subsequently, the obtained libraries were analyzed using metagenomics following the protocol by Hillman et al. (2024) on the GALAXY platform (v24.1.3.dev0).