title: GSM8703962: GSC 83 cells, Control, polysome; Homo sapiens; OTHER
library_construction_protocol: Subsequently, the cells layered onto a sucrose gradient tube ranging from 10% to 45%, then centrifuged at 36,000 rpm using a Beckman SW-41Ti rotor for 3 hours at 4°C. Peak fractions were isolated based on A260 optical density using a gradient fractionation station. mRNAs associated with ribosomes were extracted as polysome fractions and purified using TRIzol Reagent. Poly-A selected mRNAs were subsequently isolated and utilized for library preparation. RNA libraries were prepared by LC-Bio Technology CO., Ltd(hangzhou, China).