title: scRNAseq of gallus gallus: Multiplex of CD3+ T cells from PBMC and Thymus of 3 day-old TCR C KO, TCR Cy KO and WT chickens
design: scRNAseq analysis of chicken CD3+ T cells from PBMCs and Thymus comparing TCR C KO, TCR Cy KO and wild type chickens at the age of 3 days-post hatch, 14 days-post hatch and at embryonic day 18. Cells were sorted, then multiplexed using BD Flec Singel-Cell Multiplexing Kit and then BD Rhapsody WTA Reagent Kit was used for preparing the smples and Library for whole transcriptomic analysis. The samples were sequenced on NovaSeq X