title: GSM8909516: MCF7_NT1_Phospho-Ser2POL2_rep1; Homo sapiens; ChIP-Seq
library_construction_protocol: Chromatin immunoprecipitation followed by sequencing (ChIP)-seq was performed as previously described (Schmidt, D. et al. Methods 48, 240–248 (2009). In brief, cells were fixed in 1% formaldehyde for 10 min and quenched with 0.125M glycine. Nuclear lysates were extracted as previously described and sonicated for 15 cycles (30 sec on/30 sec off) using the Bioruptor® Pico (B01060001, Diagenode). Per ChIP, 50 µL of magnetic Protein A beads (10008D, Thermo Fisher Scientific) were conjugated to 5µL of ERa, TRIM24, H3K23ac, H3K27ac, pSer2-RNApolII-CTD antibodies. Illumina