title: GSM8665986: mm61_hsNUP98r_RNA_vehicle_54d_REP1_231031_GCCAAT_S3; Homo sapiens; RNA-Seq
library_construction_protocol: For RNA isolation from cell lines, 1x10^6 cells were lysed in 350 µl of RLT buffer and RNA was isolated using the RNeasy Mini Kit (Qiagen) according to manufacturer's instructions for cell line experiments. 1 ug RNA was used as the input quantity for library construction. Poly-A tail selection and library preparation were performed using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs, Ipswich, MA).