title: GSM9267957: WT_iPSC_1; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from the samples using the  RNA-Quick Purification Kit (Yishan Bio, RN001), followed by library preparation and sequencing on the Illumina platform. Messenger RNA was purified from total RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, amplification, and purification.