title: cytb MiFish metabarcoding of Qiagen DNEasy filtered water extraction blank: Laboratory control sample
design: Extracted DNA from reagents using the Qiagen DNEasy kit operating as negative controls were amplified with cytb metabarcoding primers (Snyder and Stepien et al. 2020) targeting fsh species with Nextera stye overhangs, and dual indexed with Nextera indices following the Illumina 16S metagenomics protocol (Illumina Part# 15044223 Rev B). Samples were 250 bp paired-end sequenced on an Illumina MiSeq v3.