title: GSM8775084: OCIAML3 LTR12C KD CUT&RUN H3K27ac R1; Homo sapiens; OTHER
library_construction_protocol: 500000 live cells per reaction were pelleted and digestion was carried out with pAG-MNase following the kit manufacturer's protocol (75). Library preparation was  carried out per the NEBNext Ultra II DNA Library Prep Kit for Illumina.5-10ng of DNA was used as starting material and libraries were amplfied using 13 cycles on thermocycler. Libraries were quality assessed using D1000 DNA screen tapes on an agilent 4200 TapeStation. Libraries were sent for Novaseq sequencing.