title: Genome sequence of Stenotrophomonas sp. isolated from a red-backed salamander at Paul Smiths Forest Experimental Demonstration Area
design: gDNA was extracted was from an overnight culture generated by inoculating a single bacterial colony into tryptic soy broth (Hardy Diagnostics) and incubating at 25C overnight. gDNA was extracted using the ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research). Library preparation, sequencing and genome assembly were performed by Plasmidsaurus (Eugene, OR, USA). Sequencing libraries were prepared using Rapid Barcoding Sequencing Kit version SQK-RBK114.96 (Oxford Nanopore Technologies) with no fragmentation or size selection of DNA. Sequencing was conducted on the PromethION P24 platform (Oxford Nanopore Technologies), equipped with R.10.4.1 flow cells and base calling performed using Dorado v7.1.4 on super-accurate mode (Oxford Nanopore Technologies).