title: GSM8979868: CD45 cells, treatmen 1; Mus musculus; RNA-Seq
library_construction_protocol: MC38 cells (5×105) were subcutaneously injected into the right flank of mice. The tumor volume was measured twice a week and calculated by length × width × height/2. After the tumors were established, mice received intraperitoneal injections of PBS, TIM3-ProIL2V2, respectively, every 3 days for a total of two injections unless otherwise indicated. We used the Chromium Next GEM Single Cell 3' Kit (10x Genomics) to generate scRNA-seq libraries according to the manufacturer's protocol. FACS sorting of cells from three biological replicates labeled with CD45 Antibody (BioLegend). The sorted cells were loaded onto a Chromium single-cell chip to generate barcoded nucleic acid fragments. The amplified cDNA was used to generate gene expression libraries. These libraries were sequenced on an Illumina NovaSeq 6000.