title: scRNAseq of Podocoryna genome strain mother: female non-reproductive polyps
design: 40 female feeding polyps were collected in 2 mL lo-bind tubes containing 1 mL Ca/Mg-free artificial seawater for 10 minutes at room temperature, returned to 1 mL artificial seawater , and mechanically dissociated using a 25G needle and 1 mL syringe. Cell suspensions were filtered through 100 micron and 40 micron cell strainers into new 2 mL tubes, centrifuged at 0.5g for 5 minutes and resuspended in 200 uL 1X PBS. Cell suspensions were filtered in 100 and 40 micron strainers before processing with the 10X Genomicss Chromium instrument and Dual index Single-Cell 3 Reagent kit (V3.1).