title: Leaf age determines flood resilience through distinct submergence and recovery effects
design: Leaf age determines flood resilience through distinct submergence and recovery effects
library_construction_protocol: leaf blades of leaf 3 (old leaf) and leaf 7 (young leaf) were harvested before submergence, after 2 and 4 days of submergence, and after 1, 3, 6, and 24 hours of recovery (Figure 1). 8-16 leaf blades were pooled together per biological replicate. For the Col-0 and bzip28/60 RNA-seq, Arabidopsis plants were harvested before submergence, after 1 and 4 days of submergence, and after 3 hours of recovery in the light. Old (leaves 1-5) and young (leaves 6-10 and the meristem) halves of the rosettes were harvested separately. NA Pots with seeds were germinated and grown in a growth chamber (20°C, 200 μmol m‐2 s‐1 photosynthetically active radiation (PAR), a 9-hour photoperiod from 8:00 to 17:00, and 70% relative humidity). Pots were thinned when seedlings were at 2-leaf stage (with 2 true leaves and 2 cotyledons), so there was only one seedling per pot. Plants were grown to the 10-leaf stage before they were used for experiments.  Flooding treatments were as described previously (Rankenberg et al. 2024, https://doi.org/10.1016/j.xplc.2024.100848) NA RNAseq experiments, RNA was isolated using the RNeasy Plant Mini Kit (Qiagen), residual genomic DNA was digested using on-column DNase-I digestion (Ambion).  TruSeq stranded mRNA 