title: GSM8807115: eHAP iCas9, Control Day0, Replicate 2; Homo sapiens; OTHER
library_construction_protocol: Genomic DNA was extracted from 500M cells per condition using a Quiagen Blood Maxi kit, per manufactururs protocol. Extracted genomic DNA was used as template for a primary PCR amplification of the guide-harboring locus. This  amplicon was purified by gel extraction, concentrated, and used for a secondary PCR with illumina indexes unique to each sample.