title: GSM8775291: TSC differentiation into EVT, Control, KD, rep3; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNAs were extracted using the RNeasy plus mini kit following the manufacturer's instructions. RNA-seq libraries were generated with 500 ng of total RNA using NEB Next Ultra II RNA Library prep kit (NEB, E7770) following the manufacturer's protocol. mRNAs were enriched from total RNAs with Magnetic mRNA Isolation Kit (oligo(dT) beads) (NEB, E7490), and then cDNAs were synthesized using random primers. cDNAs were purified by NEBNext Sample Purification Beads (NEB, E7767). End prep reactions were performed followed by ligation with a sample-specific adaptor and index.