title: Demultiplexed 12S rRNA sequences from seawater sampled in the Dongsha Atoll, Taiwan
design: Seawater was filtered using GF/F glass microfiber filters. DNA extraction was performed with the GeneMark Easy Tissue & Cell Genomic DNA Purification Kit. A two-step PCR approach was employed, targeting the mitochondrial 12S rRNA gene with primers MiFish-U-F (5'-GTCGGTAAAACTCGTGCCAGC-3') and MiFish-U-R (5'-CATAGTGGGGTATCTAATCCCAGTTTG-3'). The target region (160-190 bp) was excised from an agarose gel following electrophoresis and purified using the GeneMark DNA Clean Kit. In the second amplification step, an 18-base pair barcode sequence was added to both ends of the amplicon. Next-generation sequencing was performed at Genomics, Taiwan, using the Illumina MiSeq platform.