title: GSM8646890: primary endometriotic stromal cells, TCF21, ChIP; Homo sapiens; OTHER
library_construction_protocol: CUT & Tag library was prepared by NovoNGS® CUT&Tag High‐Sensitivity Kit (Novoprotein). In brief, approximately 1 × 105 endometriotic stromal cells were isolated and incubated with NovoNGS® ConA Beads. Subsequently, antibodies and ChiTag transposons were sequentially attached to the chromatin. Following this step, DNA fragments were obtained, amplified, and purified. Genomic fragments with adapters at both ends were enriched by PCR and sequenced by Novogene Bioinformatics Technology on the HiSeq 2500 platform (Illumina). Trimmed reads were aligned to hg38 using Bowtie2 and peaks were called by MACS2. Peak distribution at genomic regions of interest was depicted using the Integrative Genomics Viewer (IGV).