title: GSM9247230: PBMC, NHD, 6; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was isolated from PBMCs, using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's protocols. Genes encoding VH and part of the first constant domain of the heavy chain (CH1) domain were amplified using specific primers. NGS sequencing was performed using NovaSeq6000 SP chip (250PE).