title: DNA-Seq of Hainan gibbon: fecal sample
design: Sequencing libraries were constructed by using an NEB Next Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, USA) following the manufacturers instructions and index codes were added to attribute sequences.Briefly, the genomic DNA sample was fragmented by sonication to a size of 350 bp.DNA fragments were end polished, A-tailed, and ligated with the full-length adapter, followed by PCR amplifications with 12 cycles for each sample.The PCR products were purified by the AMPure XP system (Beckman Coulter, USA) to finish the library construction and the DNA concentration was quantified using a Qubit 2.0 Fluorometer.The constructed libraries were analyzed for size distribution on an Agilent 2100 Bioanalyzer (Agilent, USA) and quantified by real-time PCR (concentration >2 nM).