title: GSM9269354: MCMV_Acute 2; Mus musculus; RNA-Seq
library_construction_protocol: RNA was isolated with NucleoSpin® TriPrep (Macherey-Nagel®) isolation kit according to manufactures instructions. RNA-seq libraries were generated using the TruePrep DNA Library Prep Kit for MGI (VAZYME) as per the manufacturer's recommendations. Single-stranded circle DNA molecules where then replicated via rolling cycle amplification, and a DNA nanoball (DNB) which contain multiple copies of DNA was generated. Sufficient quality DNBs are then loaded into patterned nanoarrays using high-intensity DNA nanochip technique and sequenced through combinatorial Probe-Anchor Synthesis (cPAS) on DNBSEQ (MGI) sequencing platform in a single end 1x75 base pair run.