title: GSM9043964: AMs, Control2; Homo sapiens; RNA-Seq
library_construction_protocol: Total RNA was extracted from sorted alveolar macrophages using TRIzol reagent (Invitrogen) according to the manufacturer's protocol, followed by purification with the RNeasy Mini Kit (Qiagen). RNA integrity was assessed using the Agilent 2100 Bioanalyzer. RNA-seq libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) following the manufacturer's instructions. Briefly, poly(A)+ mRNA was enriched using oligo(dT) beads, fragmented, and reverse-transcribed into cDNA. After adaptor ligation and PCR amplification, libraries were quantified and sequenced on an Illumina platform.