title: The MURAL collection of prostate cancer patient-derived xenografts enables discovery through preclinical models of uro-oncology
design: RNA was isolated from PDXsusing RNeasy Micro kit (Qiagen) as per the manufacturer's instructions. Libraries were prepared using Lexogen 3′ Quantseq FWD +  and Illumina HiSeq 2000/2500 was used to sequence samples(distinct samples from different PDX generations). This spanned a combination of early generation lines (reference) and the most recent generation at the time of sequencing (working). We assigned a representative sample for each line. Where possible this was from the most recent working line in a castrate host, otherwise it was from the most recent sample maintained in a testosterone-supplemented host.