title: Hybrid genome assembly of Leptospira interrogans strain:N 116 using Illumina and Oxford Nanopore sequencing: Illumina reads
design: The Illumina sequencing library was prepared using 200 ng of total DNA and the Lotus DNA Library Prep Kit (Integrated DNA Technologies, Inc.), following the manufacturer's instructions. Sequencing was performed in a single-end 75 bp run on the Illumina NextSeq 550 system, aiming to achieve at least 50x genome coverage.