title: Anti-CD27 immunotherapy induces intratumoral immunostimulation in B-cell lymphoma: the multicenter RiVa trial (RNA-Seq Data for RiVa)
design: Anti-CD27 immunotherapy induces intratumoral immunostimulation in B-cell lymphoma: the multicenter RiVa trial (RNA-Seq Data for RiVa)
library_construction_protocol: Fresh cores of tumors cores collected in RPMI were minced into 1 mm3 pieces or smaller using a scalpel and added to Liberase™ DL (Roche, Basel) RPMI for 15 minutes at 37 oC on an orbital shaker at 250 rpm. The sample mixture was then passed through a 70 µm filter, washed four times, red cell lysis (Qiagen) undertaken, and washed twice more. Resultant single cell suspension was cryopreserved in 50% v/v human AB serum (Sigma, Burlington), 40% supplemented RPMI and 10% v/v DMSO (Sigma, Burlington). Total RNA was isolated and processed using the Maxwell® RSC simplyRNA Cells and simplyRNA Tissue kits (Promega, Madison) following manufacturer's instructions, and quantified and measured with Bioanalyser (Agilent Biosystems, Santa Clara). Sample library preparation was performed and quality checked by the Oxford Genomics Centre (OGC).