title: GSM9010789: Bank1 Tralpha with T3 repeat1; Mus musculus; RNA-Seq
library_construction_protocol: RNAs were extracted from transfected cells using the NucleoSpin RNA kit (Macherey Nagel) according to manufacturer instruction, including the recommended step of RNase-free DNase I treatment on the extraction column. The complete elimination of contaminant DNA from purified RNA was achieved by repeating the same procedure. cDNAs were prepared from 1 mg of RNA (M-MLV reverse transcriptase, Promega) using random hexamers (Eurogentec) as primers in a final volume of 20 mL. Reactions were stopped by the addition of EDTA (1 mM final) and the products diluted (1/4) with water. A PCR reaction was performed using the library amplification primers to verify after agarose gel electrophoresis the amplification of amplicons. The resulting mixture of amplicons were then sequenced on a Miseq sequencer (Illumina Microkit V2 300 cycles) using the Illumina Nextera V2 index kit