title: Total RNA transcriptome sequencing of large yellow croaker
design: Total RNA was extracted from cells transfected with the recombinant plasmid after 24 hoftransfection.  respectively, following the protocol provided with the EasyPureFast Cell RNA Kit (TransGen, China),RNA samples that metquality criteria were then used for library construction and subjectedto high-throughput sequencing on the Ilumina NovaSeg 6000platform.