title: sequences of bacteria
design: Total DNA was extracted by the CTAB method, and DNA purity and concentration were detected by agarose gel electrophoresis. The diluted genomic DNA was used as the template. The V4 zone of 16 S rDNA was  amplified by polymerase chain reaction (PCR) when 515 F-806R was used as the primers. The amplified products were sequenced on the Illumina Miseq platform. The PCR protocol was as follows. The ampli fied products were denatured at 98  for 1 min and then cycled 30 times, and finally extended at 72  for 6 min. The microbial community structure was analyzed after Qubit quantification and library testing.