title: GSM9246571: Heart_AAV9-Empty Control, biol rep 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was isolated using TriFast peqGOLD (Avantor). 500 ng total RNA was used for  library preparation. 500 ng total RNA was subjected to rRNA depletion (NEBNext rRNA Depletion Kit, NEB), followed by stranded cDNA library generation (NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina, NEB) as recommended by the manufacturer apart from downscaling reaction volumes to 2/3 of the described volumes. In addition, one extra purification step (1x 'Agencourt® AMPure® XP Beads, Beckman Coulter) was performed at the end of the standard procedure. Single indexing was utilised (NEBNext Multiplex Oligos for Illumina – Set 1' (Index Primer 2,4,5,6,7,12), NEB).