title: GSM9363367: Sham 3mo; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted using the RNeasy kit (Qiagen, Germantown, MD) according to the manufacturer's instructions. Single cell suspensions were washed in PBS/2% BSA and checked for viability > 85% Directional mRNA libraries were prepared using NEBNext Kits per the manufacturer's instructions 10X Genomics NEXT GEM Kit V2, 10X genomics Library Construction kit