title: GSM8743270: cord blood CD34+ cell with NUP98::KDM5A and Cas9 + gRNA (WT1-gRNA1) batch 2 rep2 at day 39; Homo sapiens; RNA-Seq
library_construction_protocol: RNA was harvested using Quikc-RNA Miniprep Kit (Zymo Research R1055). RNA was then NanoDrop2000 (ThermoFisher) and quality checked by the 2100 Bioanalyzer RNA 6000 Nano assay (Agilent) or 4200 TapeStation High Sensitivity RNA ScreenTape assay (Agilent) prior to library generation. Libraries were prepared from total RNA with the TruSeq Stranded Total RNA Kit, with Ribozero Gold (Illumina, 20020598), Ovation RNA sequencing system v2 (Tecan, 7102-32), or Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (Illumina, 20040529) as indicated for each sample. Libraries were analyzed for insert size distribution using the 2100 BioAnalyzer High Sensitivity kit (Agilent), 4200 TapeStation D1000 ScreenTape assay (Agilent), or 5300 Fragment Analyzer NGS fragment kit (Agilent). Libraries were quantified using the Quant-iT PicoGreen ds DNA assay (ThermoFisher) or by low pass sequencing with a MiSeq nano kit (Illumina). Paired end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina).