title: RNA-Seq of BMDCs
design: mRNA was enriched by magnetic beads with Oligo (dT) and then randomly interrupted by fragmentation buffer. The first and second cDNA strands were synthesized and purified. After end repaired and adenylation of the 3'-ends of the DNA fragments, Illumina sequencing adapters were ligated, and the AMPure XP beads were used for fragment size selection. Finally, cDNA library was obtained by PCR enrichment.