title: ="16S sequencing of mouse: "&B36
design: DNA was extracted from fecal pellets using QIAamp PowerFecal Pro DNA extraction kits (Qiagen, USA); DNA quality and quantity was assessed via Nanodrop. Fecal DNA was utilized for full length 16S ribosomal RNA gene amplicon sequencing at the University of Illinois W.M. Keck Center for Comparative and Functional Genomics. 16S amplicons were generated with barcoded primers from PacBio targeting the entire 16S gene (forward sequence, AGRGTTYGATYMTGGCTCAG; reverse sequence, RGYTACCTTGTTACGACTT) and Roche KAPA HiFi Hot Start Ready Mix. The amplicons were converted into a sequencing library with a SMRTbell Express Template Prep Kit 3.0, and the library was then sequenced on a single SMRTcell 8M using a PacBio Sequel IIe platform in Circular Consensus Sequencing (CCS) mode and a 15-hour movie run time. CCS libraries were analyzed for read quality and demultiplexed on SMRTLink version 11.1, creating raw sequencing reads as FASTQ files for each fecal sample.