title: GSM9249287: HUVECs, FXR, CUT&Tag; Homo sapiens; OTHER
library_construction_protocol: CUT&Tag assay was performed using the Hyperactive Universal CUT&Tag Assay Kit for Illumina Pro (Cat#TD904, Vazyme, China) according to manufacturer's instructions. For CUT&TAG, DNA was purified using phenol-chloroform-isoamyl alcohol extraction and ethanol precipitation. The libraries were amplified by PCR with a universal i5 and a uniquely barcoded i7 primer. Size analysis of the finished library was conducted using the Agilent 4200 TapeStation system (Agilent Technologies, Santa Clara, CA, USA). Finally, sequencing was performed with the Illumina NovaSeq 6000 platform and 150 bp paired-end reads were generated for the subsequent analysis.