title: 16S rRNA sequences: lower teeth dental plaque of patient 2
design: Plaque sample was suspended in sterile saline and transferred to a PowerBead (Qiagen) tube for mechanical bacterial lysis. Supernatant was next treted with Inhibitor Removal Technology reagent and eluted through a MB Spin column. Total genomis DNA was extracted using the DNeasy Power Soil PRO Kit (Qiagen).