title: GSM9246878: Follicular B cells, 18hr CpG, WT 1; Mus musculus; RNA-Seq
library_construction_protocol: Total RNA was extracted from sorted cells with Monarch® Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer's instruction. Quality controls were performed on Bioanalyzer 2100 using RNA Integrity Number (RIN) > 8 (Agilent Technologies, Santa Clara, CA, USA) and Qubit V4 (Invitrogen Thermo Fisher Scientific, Waltham, MA, USA). NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs Inc.) was employed with the NEBNext Multiplex Oligos for Illumina (New England BioLabs Inc.) and NEBNext® Poly(A) mRNA Magnetic Isolation Module for cDNA synthesis with the addition of barcode sequences. The sequencing of the pre-pools was performed using the NextSeq2000 (Illumina, San Diego, CA, USA) with the P2 reagents kit V3 (100cycles; Illumina). Samples were processed starting from stranded, single-ended 120bp-long sequencing reads.