title: scRNAseq of Podocoryna genome strain: male non-reproductive polyps, ACME dissociation
design: 300 male feeding polyps for ACME method were collected in 2 mL lo-bind tubes and relaxed in 4% MgCl2 for 5 minutes on ice before a wash with 4% MgCl2 and N-acetyl cysteine buffer. The wash buffer was replaced with fresh ACME buffer, and after allowing 1 minute for ACME buffer to permeate the sample, tissues were manually dissociated by pipetting. Cell suspensions were filtered in 100 and 40 micron strainers and sorted using FACs before freezing and shipping for processing with the 10X Genomicss Chromium instrument and Dual index Single-Cell 3 Reagent kit (V3.1). More details available here: https://protocols.io/view/podocoryna-acme-cell-dissociations-draft-v1-0-may-ddjw24pe.