title: GSM8776770: 5/6 Nx animal 1 fed a 2% phosphorus and 2.5% protein diet; Mus musculus; RNA-Seq
library_construction_protocol: Animals fed one of four different diets for 30 days. These diets were: HPi-Lp diet (TD.180077) containing 2% phosphorus and 2.5% protein, HPi-Hp diet (TD.220202) containing 2% phosphorus and 18% protein, LPi-Lp diet (TD.230207) containing 0.3% phosphorus and 2.5% protein, and LPi-Hp diet (TD.230584) containing 0.3% phosphorus and 18% protein. All diets were purchased from Inotiv Envigo. The aorta was collected and total RNA was isolated using RNeasy Mini Kit. Messenger RNA was purified from total RNA using poly-T oligo-attachedmagnetic beads. After fragmentation, the first strand cDNA was synthesizedusing random hexamer primers, followed by the second strand cDNAsynthesis using either dUTP for directional library or dTTP for non-directional library.