title: RNA-Seq of Trachemys scripta elegans: adult female heart
design: Frozen heart samples (~100 mg) of control and 20-hour anoxic red-eared slider turtles were sent to the BC Cancer Agency (Vancouver, BC, Canada) for small RNA extraction and sequencing. Total nucleic acid (TNA) was isolated and miRNA libraries were constructed. Small RNA sequencing was performed using via an Illumina NextSeq 2000 which generated 100 bp single ends.