title: GSM8978530: ChIP-Seq of NR1D1 in Hep3B cells (Chip); Homo sapiens; ChIP-Seq
library_construction_protocol: Hep3B cells were fixed in 1% formaldehyde for 10 mins at room temperature, after which 125 mM glycine was added and left for 5 min to terminate the crosslinking reaction. After ChIP lysis buffer (5 mM PIPES, pH 8.0, 85 mM KCl, 0.5% NP-40, protease inhibitors) treatment, the nuclear pellets were collected by centrifugation at 2000×g for 5 min at 4 ℃.The chromatin was sonicated to an average DNA fragment length of 200-500 bp. The high-throughput DNA sequencing libraries were prepared by using VAHTS Universal DNA Library Prep Kit for Illumina V3（Catalog NO. ND607, Vazyme）.The library products corresponding to 200-500 bps were enriched, quantified and finally sequenced on NovaSeq 6000 sequencer (Illumina) with PE150 model.