title: RNA-Seq of Mus musculus: CTL2 Colon tissue
design: Colon samples from day17 AOM/DSS-treated ChREBP CTL and KO group mice (n=3 per group) were used for RNA isolation.Poly-A mRNA was purified with poly-T magnetic beads, fragmented, converted to cDNA, and library-prepped. Libraries were quantified, pooled into ssDNA circles, converted to DNA nanoballs (DNBs) via RCR, and sequenced as single-end 50 bp reads on the BGISEQ-500 platform using the Combinational Probe-Anchor Synthesis method.