title: GSM8980142: GSC1_Stem_H3K27ac; Homo sapiens; ChIP-Seq
library_construction_protocol: For CTCF, 3 million cells were crosslinked and lysed using the truCHIP Chromatin Shearing Kit (Covaris). For RNA pol2 and H3K27ac, For each biological replicate, 5 million cells were pelleted and sent to Active Motif for chromatin immunoprecipitation, library preparation, and bioinformatic analysis. For CTCF, Immunoprecipitation for CTCF and ChIP-seq library preparation was performed using the ChIP-IT High Sensitivity Kit (Active Motif) and Next Gen DNA Library Kit (Active Motif), following the manufacturer's instructions.