title: GSM8696267: Donor 01_HKSA stimulation_plate2; Homo sapiens; Staphylococcus aureus subsp. aureus USA300; RNA-Seq
library_construction_protocol: PBMCs isolated from 6 healthy donors were separated via negative selection into CD4+ and CD4- fractions. After irradiation of CD4- cells, both fractions were re-mixed at a 1:1 ratio and stimulated with heat-killed S. aureus or  α-CD3/CD28 antibodies. After 24 h stimulation, for each stimulus CD4+ T cells co-expressing OX40 and CD137 (AIM+)  were sorted in single wells of 2 replicate 384-well plates from Single Cell Discoveries precast for SORT-seq. From each plate a library was generated by Single Cell Discoveries (https://www.scdiscoveries.com/). https://www.scdiscoveries.com/