title: PacBio HiFi whole genome sequencing of Aphelocoma coerulescens, whole blood
design: gDNA quality assessed with Femto Pulse System (Agilent); sheared using Megaruptor 3 (Diagenode) with target size 14-15 kb; libraries prepared using SMRT Bell prep kit v3 (Pacbio); sample libraries were size-selected from 6-8 kb using Blue Pippin instrument (Sage); Each sample was sequenced on 2 cells of a Sequel IIe instrument using PacBio sequencing kit v2 and Sequel II binding kit 2.2.