title: RNAseq of Gryllodes sigillatus: adult female ovaries
design: RNA was extracted using a Trizol and 1-bromo-3-chloropropane phase separation using standard procedures, followed by a DNAse treatment using a TURBO DNA-free kit. Only RNA with final 260/230 and 260/280 values higher than two were used for RNA sequencing. RNA was processed with an Illumina TruSeq Stranded mRNA Kit with polyA enrichment, and were sequenced as paired end 150bp reads on a NovaSeq 6000.