title: Illumina sequencing of liver
design: For short-read sequencing, a short fragment library with a size of 350 bp was constructed using the NEBNext Ultra DNA Library Prep Kit for Illumina (E7645L, NEB, USA) and was then sequenced on an Illumina NovaSeq 6000 Sequencing System (Novogene Biotechnologies, Inc., Tianjin, China). Raw reads with >10% unknown bases or with >20% low-quality bases and all adaptor sequences produced in the PCR process will be removed as low-quality sequences.