title: GSM9268542: CT26_lucif2; Mus musculus; RNA-Seq
library_construction_protocol: Following the treatment period, total RNA was isolated from all cell samples using TRIzol reagent, strictly adhering to the manufacturer's recommended protocol to ensure high-quality RNA integrity. Sequencing libraries were constructed, with the SMARTer kit employed for certain samples requiring specific adapter handling. High-throughput sequencing was then performed on the Illumina HiSeq platform using a paired-end sequencing strategy. Raw sequencing data underwent a rigorous quality control pipeline: adapter sequences and low-quality bases, which were predominantly concentrated at the 3' ends, were dynamically trimmed using Skewer.