Low coverage genome of Acinetobacter guillouiae
Single colonies were picked from each plate and streaked onto tryptic soy agar to ensure isolation.  The plates were incubated at 28 C for 18 hours. For each strain, 2 mL of tryptic soy broth was inoculated with an isolated colony.  The cultures in suspension were incubated at 28 C with shaking at 250 rpm until turbid then centrifuged at 3,220 rcf for 5 minutes.  Without disturbing the bacterial pellets, the supernatant in each tube was decanted. To remove media and periplasmic proteins, the bacterial pellets were washed in 300mM sucrose that had been sterilized with a 0.2-micron polyethersulfone (PES) filter.  The bacterial pellets were resuspended in 1 mL of 300 mM sucrose and suspensions were centrifuged at 3,220 rcf for 5 minutes. Without disturbing the bacterial pellets, the supernatant in each tube was decanted.  The process was repeated for a total of two washes.  Then the bacterial pellets were frozen at -20 C for a minimum of 20 minutes to aid cellular lysing and to provide a storage temperature with minimal enzymatic activity. High-molecular-weight genomic DNA was extracted using the MasterPure Yeast DNA Purification Kit (Biosearch Technologies Genomic Analysis by LGC MPY80200) from the sucrose-washed bacterial pellets according to manufacturer's specifications (see Supplemental Methods for minor deviations) and sent to SeqCoast Genomics for short read whole genome sequencing with Illumina or long read sequencing with Oxford Nanopore Technologies.