16S metagenomics
DNA samples from the EPF infected midgut and hindgut tissues were subjected to amplification of the 16S rRNA V4-V5 region with the PCR primer set 515f / 806r, followed by DNA libraries construction and next generation sequencing (NGS). The sequencing library was sequenced using the Illumina MiSeq platform to generate 2  300 bp paired-end reads.