PANFR0123_T2 organoids_+IFNg
Organoids were treated with vehicle (PBS) or IFNγ (100 ng per mL; Peprotech) for 36-40 hours. Total RNA was harvested using the Pure Link RNA Mini Kit (Thermo Fisher, 12183018A) according to manufacturer instructions using the Trizol Plus Whole Transcriptome protocol. cDNA was generated using the NEBNext Ultra II Directional RNA with rRNA Depletion Library Prep Kit for Illumina (NEB) according to manufacturer instructions. Samples were sequenced on a NovaSeq SP (Illumina) with 150nt PE reads at 20M reads per sample.