RNA of amoeba infected with parasitic bacteria
5106 pure amoebae cells with bacteria in the logarithmic phase, which were preserved in 1 mL of TRIzol reagent (Invitrogen, Carlsbad, USA) and frozen using liquid nitrogen. Majorbio Technology Co., Ltd (Shanghai, China) conducted the bioinformatics analysis using the Illumina NovaSeq 6000 platform (Illumina, CA, USA). We performed referenced transcriptome analysis on amoebae D. discoideum, aligning the data with the reference genome sequence (GCF_000004695.1) using HISAT2 (http://ccb.jhu.edu/software/hisat2/index.shtml).