Single cell RNA-seq with immune profiling of homo sapiens: T cells in Stevens-Johnson syndrome and Morbilliform Drug eruption
Libraries prepared according to 10X Genomics Single Cell 5′Kit v1 Immune Profiling with Feature Barcoding kit. BRI-793 contain the gene expression libraries, BRI-794 contain the feature barcode libraries, BRI-795 contain the VDJ libraries. Demultiplex samples using the feature barcode libraries according to: #12 MDE3_Skin GTCAACTCTTTAGCG; #17 HC_Skin_2 TTCCGCCTCTCTTTG; #11 MDE3_Blood AGTAAGTTCAGCGTA; #13 HC_Blood_1 AAGTATCGTTTCGCA; #15 HC_Blood_3 GGTTGCCAGATGTCA; Assign antibody derived tags using the feature barcode libraries according to: CD56 TCCTTTCCTGATAGG; CD45RA TCAATCCTTCCGCTT; CD45RO CTCCGAATCATGTTG; CD335 ACAATTTGAACAGCG; CD103 GACCTCATTGTGAAT; CD69 GTCTCTTGGCTTAAA; CD62L GTCCCTGCAACTTGA; CCR7 AGTTCAGTCAACCGA; LAMP-1 CAGCCCACTGCAATA; FAS CCAGCTCATTAGAGC; IL-7Ra GTGTGTTGTCCTATG; CD4 GAGGTTAGTGATGGA; CD8 GCGCAACTTGATGAT