Whole exome sequencing  of human esophageal cancer cell line TE-1
Genomic DNA is randomly fragmented to 180-280bp, followed by end repair, A-tail addition, and linker ligation. The fragments with linkers are then subjected to PCR amplification, fragment screening, and purification. The pre-library was hybridized with biotin-labeled probes, and the exons were captured by magnetic beads containing streptavidin. Subsequently, the non-hybridized fragments were eluted, the probes were digested, and the captured library was enriched by PCR.