RNA-Seq of Trichodesmium erythraeum in nutrient-amended culture experiment
At the times of harvest, 25 mL subsamples of each culture flask were filtered onto polycarbonate filters.  Prokaryotic RNA was extracted with the Qiagen RNeasy Mini Kit.  Ribosomal RNA was removed using the Ribo-Zero Magnetic Kit for bacteria.  Purified prokaryotic mRNA was concentrated using the RNeasy MinElute Cleanup Kit.  Sequencing of 30M, 100 bp, PE reads from each sample was performed on an Illumina HiSeq.