PANFR4116_9_FLEX
Single-cell Gene Expression Flex (10X Genomics). Briefly, samples were divided into 25 mg sections and minced finely with razor blades before overnight fixation in 4% formaldehyde (Fisher Scientific) at 4C. Twenty-four hours post fixation, samples were dissociated using Liberase TH (Roche) in a heated gentleMACs Octodissociator (Miltenyi), following recommended parameters. Probe hybridization and library preparation were conducted following manufacturer instructions. Dual-index, paired-end reads were sequenced on a NovaSeq S2 (Illumina) at 20,000 read pairs per cell following recommended sequencing parameters.