CFIAFB20240043_L. monocytogenes
Brain Heart Infusion broth was inoculated with a single colony of bacteria and incubated overnight at 35C. Genomic DNA was isolated from 1ml of culture using the Qiagen DNeasy Blood & Tissue kit (Qiagen, Canada). DNA was then quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing library was constructed from 400 ng of gDNA using a Rapid Barcoding Sequencing Kit (SQK-RBK004, Oxford Nanopore Technologies, UK ) according to manufacturers instructions. Nanopore sequencing was performed using a FLO-MIN106D (R9.4.1) flow cell on a MinION Mk1B device (Oxford Nanopore Technologies).