replicate-2 of PBS control
Mice were anesthetized, and their backs were shaved, followed by application of depilatory cream to remove any remaining fur. Each day for 14 days total, 5% IMQ cream (62.5 mg, Patterson Veterinary Supply, cat. num. 07-893-7787) was applied to the backs of the mice. On the 3rd day of IMQ application mice were subcutaneously injected with sterile saline as a control spread evenly throughout the back (n = 12 per group). Skin was harvested at the endpoint of day 14. This is replicate-2 of PBS control.