RNA-Seq of Homo sapiens:adipose
Total RNA was extracted (TaKaRa, 9108), rRNA was removed using probe hybridization (MGI, 1000005953), and the RNA was purified. RNA library construction was performed using an RNA library preparation kit (MGI, 1000006383). The samples were sequenced using a gene sequencer (MGISEQ-2000) and an MGISEQ-2000RS high-throughput sequencing reagent kit (FCL PE150) (MGI, 1000012555) to determine the transcriptomic gene expression profiles of H1 cells, H1-iMSCs, and bone marrow-, adipose-, and umbilical cord-derived MSCs.