16S MGx-Seq from mine tailings
The total DNA was extracted from 5g of soil using method adapted from Verma et al. 2017. The DNA was stored at -20 C. Amplicon metagenomics performed by Macrogen Europe (Amsterdam, Netherlands). Briefly, PCR of the V3-V4 region was performed using broad range primers (341F and 805R). Libraries were pooled according to Illumina's protocol and sequenced using MiSeq platform at 2×300 paired-end format with an output of at least 100,000 tags for each sample.