Viral metagenome from Menes Caldera methane oxidizing enrichment
Spent enrichment medium was filtered to remove cells. The viruses in the filtrate were concentrated and purified on an Optiprep density gradient. Purified viruses were treated with  RNase-free DNaseI (New England Biolabs M0303L) to remove free DNA. Viral DNA was extracted using Promega Wizard PCR Preps DNA Purification Resin (Cat. A7181). Sequencing libraries were prepared using the Illumina DNA Prep library kit (Cat 20018705) with IDT for Illumina DNA/RNA UD Indexes Set C (Cat. 20042666). Paired-end sequencing was performed at the University of Southern California Keck School of Medicine using an S1 flow cell for 300 cycles on the Illumina NovaSeq6000 sequencing platform (v1.5 reagents).