male pupa dsx amplicon sequencing sample 6
PCRs were performed under non-saturating conditions to amplify a 286-bp fragment spanning the doublesex T1 target site, using the primers dsx_poolamp_F and dsx_poolamp_R, which contain partial Illumina adaptors. The reactions were then PCR purified using the Monarch PCR & DNA Cleanup Kit (NEB). Next, the DNA concentration of each sample was quantified using a NanoDrop spectrophotometer and normalised to 20ng/L before being submitted for the Amplicon-EZ sequencing service (Genewiz).