ILB205 with pre growth in n-pentadecane and the inoculated in Minimal Medium supplemented with P. guildnii epicuticle rep2
For each condition, fungi were incubated at 26 C in three biological replicates, and fungal mycelia were collected for RNA extraction after 4 days post inoculation (dpi). Each fungal sample was ground with a RNAse-free mortar and pestle, which had been pre-cooled with liquid nitrogen. Total RNA was extracted using RNAeasy Plant isolation kit (Qiagen, Germany). Polyadenylated mRNA was strained from the total RNA and cDNA libraries were prepared using the Truseq RNA Library Prep mRNA kit (Illumina, San Diego, USA) following the manufacturers instructions. Constructed libraries were sequenced at the 150 bp PE with Illumina Novaseq6000 (Illumina), performed at Macrogen Inc, Korea.