Promoter capture of Triticum turgidum cv. Kronos. EMS-induced mutations
The promoter capture probes were ordered from Roche (SeqCap EZ Prime Developer Probes 96). For the capture, 24 DNA libraries were pooled and mixed with blocking oligos, denatured at 95 C and hybridized to the probes at 48 C. The captured DNA was amplified using KAPA HiFi HotStart ReadyMix and purified with Agencourt AMPure beads. Two captures were sequenced each time in one lane of Illumina NovaSeq S4 (PE150).